LOCUS       DBNGZE010000001        11355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_625199, whole genome shotgun sequence.
ACCESSION   DBNGZE010000001 DBNGZE010000000
VERSION     DBNGZE010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_625199"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="groL"
                     /locus_tag="AC16FV_00005"
     CDS             complement(<1..1373)
                     /gene="groL"
                     /locus_tag="AC16FV_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801298.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR2848476.1"
                     /translation="MAHKQILFRSAAREKILRGATALVDAVRVTLGPKSKSVLIQRKW
                     GTPLVCNDGVTIAKETELADPEEDLGARMLRQAAERTCEAVGDGTSTSTILAHAILAD
                     GIRNVVAGASAIDLKRGLDRGLKTTIDSLRTLSRPVQTRLEKAQVATNSAHNDPAIGE
                     LVAEAMEKVGGEGVITVEESKTTETVLEVVEGMQFDRGFISPYFITNAEKMEAVLEDA
                     YMLLSDRKVGNLKDLLPLLEQVAKVGRPILFVAEDVEGEALATLIVNQIRAVLRSCAV
                     KAPGFGDRRKEMLQDMAVLTGGQVIAEELGLKLEDVQLEQLGRAKRIVVDKDHTTIIG
                     GAGDKVAIQARVGQIRKQIDKATSDYDKEKLEERLAKLAGGVAVIRVGAPSEAEMKSR
                     KEALDDAIAATKAAVAEGIVPGGGLALLRCTEAIEREEGKGEGDERTGLQILRRALEV
                     PARQIAE"
     gene            complement(1586..1894)
                     /locus_tag="AC16FV_00010"
     CDS             complement(1586..1894)
                     /locus_tag="AC16FV_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR2848477.1"
                     /translation="MATFVMQTRISSDAARSPQALELLEKRAMEQIRAECPEVKWVHS
                     YALLGPYDYLDIFTAPDIETATKVSALIRTYGRAHSEIWAATDWSAFKEMIHGMPHAA
                     "
     gene            complement(2005..2358)
                     /locus_tag="AC16FV_00015"
     CDS             complement(2005..2358)
                     /locus_tag="AC16FV_00015"
                     /inference="COORDINATES: protein motif:HMM:NF042799.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HIR2848478.1"
                     /translation="MTTTLEPQVSQWYRHLDKGQQFYVTAIDDEEGTVEIQHFDGDVE
                     EMDLDDWRELDLEAIEPPEDWTGPVDDVERDDLGYTETDMKPEDWSEPLEEKSKRREG
                     TEEEEVTGEEESSEE"
     gene            2504..3490
                     /locus_tag="AC16FV_00020"
     CDS             2504..3490
                     /locus_tag="AC16FV_00020"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR2848479.1"
                     /translation="MNQDAPVLFALQASRAFGEDVARHLGIALSAHEEREFEDGEHKS
                     RPRVNVRDKDVFVIQSLYGDAQQSVNDKLVRLLFFIGAVKDASAARITAVVPYLAYAR
                     KDAKTQTRDPVTTRYVAQLFEAAGTDRVVTLDVHNLAAFQNAFRIRTDHLEATKLFVA
                     HVTPLLQSEPHITVVSPDIGGIKRAERFRQALARALGRELNLAFMEKARAKGVMRAGR
                     LAGEVVGTAIIIDDLISTGGTLLQAAIACRERGATKVYAAATHGLFIGDAGYLLSGNE
                     LDKIVITDTVPPFRLNRQLIAKKLAVVSAAPLFAEAIRRIHTGGSLVELLEA"
     gene            complement(3621..4649)
                     /locus_tag="AC16FV_00025"
     CDS             complement(3621..4649)
                     /locus_tag="AC16FV_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848480.1"
                     /translation="MKLMSRQDTVPDQKARIAFLRQPRAYPEKPAHVETIETHMSWVF
                     LTPRHAFKLKKPVHYDYLDFSTVAARKMNCEEELRLNQRLAPNVYLDVVPLALDSDGE
                     MRLDGQGEILDWLVMMRRLPAERMLDYLIRKRVLEPMDTHAVAEVLARFYRESAALEI
                     SGEEYRRQYEHKVQINHDALSDPAYGLSAALVTAVHETQIEFLHAAPELFERRAADGR
                     IIEGHGDLRPEHVCVESLPVIFDRLEFNRSFRLIDPADELAFLAMECERLGAPQVEPE
                     LFGAYRRFTGDYPPPQLVYFYKAYRTCLRARLAIWHVHDLSQSAWPRWRGVAGEYLRL
                     AESYSGKF"
     gene            complement(4646..5308)
                     /locus_tag="AC16FV_00030"
     CDS             complement(4646..5308)
                     /locus_tag="AC16FV_00030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR2848481.1"
                     /translation="MATREREQTVSIPMDSAIHQGALAIPPAAKGLVLFAHGSGSGRL
                     SPRNNYVAGVLRESGVATLLFDLLTEKEDRIYENRFNIDLLTRRLLLATQWAGQQTAT
                     RGLALGYFGASTGAAAALMAAAALGPEVRAVVSRGGRPDLALDDLPRVQAPTLLIVGG
                     DDTVVIGLNRVAYHELRGEKELKIIPGATHLFEEPGTLEQVATAAARWFERHLTGKGM
                     ES"
     gene            complement(5319..5957)
                     /locus_tag="AC16FV_00035"
     CDS             complement(5319..5957)
                     /locus_tag="AC16FV_00035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR2848482.1"
                     /translation="MHFRDRVDAGKQLAQALKKYQKRHGVVYALPRGGVVLGVEVARA
                     LRMPLDLLIPRKIGHPRQPEYAICAVVENGEMVCNQQEVARVDPRWFQQEVEAERQEA
                     RRRRELYLGKREPSPVEGKTAIIVDDGIATGLTMEVSIRDARRRKPAHLVVAVPVAPP
                     DTVERLSREVDEFVVLDPSPYYLGAVGAYYDHFLQVTDEEVIALLRSMTPAG"
     gene            complement(6052..6621)
                     /gene="amrA"
                     /locus_tag="AC16FV_00040"
     CDS             complement(6052..6621)
                     /gene="amrA"
                     /locus_tag="AC16FV_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR2848483.1"
                     /translation="MNNISSTLAQPHKQQLLQLAGESIKKGLCGEALSVRASDYPESL
                     RVLRATFVTLHLEAQLRGCIGTLEARRTLVEDVVSNAWSAAFRDPRFSALTWSEYERL
                     DIHISVLSLPEPLQFSSEDELLAQLRPRVDGLIIEEGYNRGTFLPSVWEQLPDPREFL
                     RQLKHKAGLPPDYWSSRIRVQRYTTESIP"
     gene            complement(6614..7399)
                     /gene="amrB"
                     /locus_tag="AC16FV_00045"
     CDS             complement(6614..7399)
                     /gene="amrB"
                     /locus_tag="AC16FV_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR2848484.1"
                     /translation="MTSVRNPAVAGLFYPDDPRELQAMVTGFLGAVAAAGAVPKAIIA
                     PHAGYIYSGPIAASAYARIRPARGRITRVVLLGPAHRVGFHGLALSSADCFQTPLGRI
                     TVDQEAVKKISRLPQVQVMDAAHAQEHSLEVHLPFLQEALGEFSLVPLVVGDAEPGEV
                     AEVLDTLWGGPETLIVISSDLSHYHDYKTAQRLDRATSQAIEQLRPEAIEYDHACGRN
                     PVNGLLQVARKRGLKARTIDLRNSGDTAGSHDRVVGYGAYVFE"
     gene            7561..8664
                     /gene="amrS"
                     /locus_tag="AC16FV_00050"
     CDS             7561..8664
                     /gene="amrS"
                     /locus_tag="AC16FV_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR2848485.1"
                     /translation="METEILDSAAVVPTRYWHKLDDGRVQCDVCPRYCKLHEGQRGLC
                     FVRGNHNGQIVLTTYGRSSGYCVDPIEKKPLNHFLPGTPILSFGTAGCNLACKFCQNW
                     DISKSRELDTLMDQASPETIARAAKALECRSVAYTYNDPVIFLEYAIDVAKACREVGV
                     KSVAVTAGYMCEEPRREFYQYMDAANVDLKAFTEEFYWKVTGAHLLPVLDTLKYLKHE
                     TKVWFELTTLLIPGLNDSEKEIEEMTQWVVENLGPDVPMHFTAFHPDWKMRDIPATPV
                     ATLIRSRKIAMKNGVRYAYTGNVHNEEGDSTYCHHCGTKLIGRDWYNMTAWNLDAQGK
                     CKACGTACAGVFEAEPGNWGARRLPVRLANFAA"
     gene            8751..9458
                     /locus_tag="AC16FV_00055"
     CDS             8751..9458
                     /locus_tag="AC16FV_00055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR2848486.1"
                     /translation="MPDAANRVFDLPEMRNRGRVFRDRTQAGAVLAGMLGEFRGSDTL
                     ILAIPAGGVPVAAEIAKQLGLTLDLAVVSKITLPWNTESGYGAVAFDGTVRLNQKLIA
                     ALGLAETAVQEGVAQTKEKVARRVKLLCRDRRLLGLTGRSVILVDDGLASSFTMHTAI
                     DALHRQAADKIIVAVPTGHAEAVARIAASVDSLYCPNIRQGMSFAVADAYEQWTDVSE
                     ATVAAIMQSFSGTQHAV"
     gene            9455..9877
                     /locus_tag="AC16FV_00060"
     CDS             9455..9877
                     /locus_tag="AC16FV_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR2848487.1"
                     /translation="MSAMRWEHFPHEADMGIRGIGHTRDEAFEQAALAMTAVITDPAQ
                     VKAAEMVEITRQAPDDELLLVDWLNALVYEMATRKLLFSRFEVHVKDHVLTARAFGEP
                     MDTSKHHMAVEIKGATYTALRVAQESNGEWVAQCIVDV"
     gene            9892..11322
                     /locus_tag="AC16FV_00065"
     CDS             9892..11322
                     /locus_tag="AC16FV_00065"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR2848488.1"
                     /translation="MDIALFSRHTDYEWEIAPHGAMRVPAVIYADEALIRAMDHKVYE
                     QVCNVATLPGIVKASYAMPDAHWGYGFPIGGVAAFDPDEGGVVSAGGVGFDISCGVRC
                     LHTGLNSEDVMAVQKSLADTLYREIPAGLGSTGTIRLNQSEMDAMLAGGAAWAIKQGY
                     GSAADLDHIEERGQMRGARPDCVSDHAKKRQRDEMGTLGSGNHYLEVQKVAQVFDTNT
                     ANVFGIRENDVVVSIHCGSRGLGHQIGTEFLKEMVIAAPRYGITLPDRELACAPINSP
                     IGEDYLGAMRAGINCALANRQILTHLVREAFSQVIPKARLPLLYDVSHNTCKVEEHVV
                     DGKRRKLYVHRKGATRAFGPGHPDSPDMLRPVGQPVLIGGSMGTSSYILVGTKKGEAL
                     SFSSACHGAGRAMSRHQAYKTWRGRQVIDELAGRGILIRTRSYRGVAEEAPGAYKDVS
                     AVVEATHAAKLARKVAKLVPLVCVKG"
BASE COUNT         2163 a         3552 c         3419 g         2221 t
ORIGIN      
        1 ttttcggcga tctgccgcgc cgggacctcc agggcgcggc ggagaatctg caggcccgtg
       61 cgctcgtcgc cctcgccctt gccctcctcg cgctcgatgg cctcggtgca gcggagcagc
      121 gccagcccgc cgcccggcac gatgccttcg gcgaccgccg ccttggtggc ggcgatggcg
      181 tcgtcgagcg cctctttcct ggacttcatc tcggcctcgg agggcgcgcc gacgcggatc
      241 acggccacgc cgccggcgag cttggccaga cgctcttcga gcttttcctt gtcgtaatcg
      301 ctcgtggcct tgtcgatctg cttgcggatc tgacccacgc gtgcctggat cgcgaccttg
      361 tcgccggcgc caccgatgat cgtggtgtga tccttgtcca ccacgatgcg cttggcgcgg
      421 ccgagctgtt cgagctgcac atcctcgagc ttgaggccga gttcctcggc gatcacctgg
      481 ccgccggtga gcaccgccat gtcctgcagc atctccttgc ggcgatcgcc gaagccgggc
      541 gccttgaccg cacaagagcg cagcaccgcg cggatctggt tgacgatcag ggtggcgagc
      601 gcctcgccct cgacgtcttc ggccacgaac aggatcggcc tgccaacctt ggccacctgc
      661 tcgagcagcg gcagcagatc cttgaggttg ccgaccttgc ggtcgctcag gagcatgtag
      721 gcatcctcca gcaccgcctc catcttctcg gcgttggtga tgaagtaggg tgagataaag
      781 ccgcgatcga actgcatgcc ctcgaccacc tcgagcacgg tctcggtggt cttggactcc
      841 tcgaccgtga tcacgccctc gccgccgacc ttctccatgg cctcggccac caactcgccg
      901 atcgccgggt cgttgtgcgc ggagttggtg gcgacctgcg ccttctcgag ccgggtctgc
      961 accggccgcg agagcgtgcg caacgagtcg atggtggttt tcaggccgcg gtcgagcccg
     1021 cgtttcaaat cgatggcact cgctccggcc acgacattgc ggatgccgtc ggcgaggatc
     1081 gcgtgcgcga ggatggtcga ggtcgaggtg ccgtcgccga ccgcctcgca ggtcctctcc
     1141 gccgcctggc gcagcatgcg cgcgccgagg tcttcctcgg gatcggccag ctcggtctcc
     1201 ttggcgatgg tcacaccgtc gttgcacacc agcggcgtgc cccattttct ctggatcaat
     1261 accgacttgg atttggggcc gagcgtcacg cgcacggcgt caaccagcgc tgttgcgcct
     1321 ctcaggattt tttcccgcgc cgctgaacgg aagagaattt gtttgtgggc cacaaccatt
     1381 ctcctttgcg gaaaccgcaa ctggcgatta gtgtcccaat ctctctcagg aaattctggc
     1441 tattccccgt acatgaacga attcacttat accccggcaa acagccggag cattgattat
     1501 tattatcaaa atccaggcac gaagcgagct ttaatctgcg ccgcgactga gtgctatccg
     1561 cgcgacagcc tcacgtttgt cccgttcagg ccgcgtgcgg catgccgtgt atcatctcct
     1621 tgaaggcgct ccagtccgtg gccgcccaga tttccgaatg cgccctcccg taagtgcgaa
     1681 tgagcgcgga taccttggtg gcggtttcga tgtccggcgc ggtgaagata tcgagataat
     1741 cgtagggacc gagaagcgcg taactgtgaa cccactttac ttcggggcat tcggcgcgaa
     1801 tctgctccat ggcgcgtttc tccagcaact ccagggcttg cggggaacgc gcggcatcgg
     1861 acgatatgcg cgtctgcatg acgaatgtag ccatgacacc ctcctttcga cttggcatct
     1921 gaccgggctg atcccgggct tcctgttcgg cagcggcggt cttcatcgcc atgccgaaat
     1981 aaattctcca cggcctgttc ggcgtcattc ttcgctgctt tcttcttccc ctgtcacttc
     2041 ctcctcctct gttccctccc tccgcttcga tttctcctcc agaggttcag accagtcttc
     2101 cggcttcatg tccgtttcgg tatagccgag gtcgtcacgc tcgacatcat ccaccggacc
     2161 ggtccagtcc tcgggcggct caatggcttc gagatcaagc tcgcgccagt catcgagatc
     2221 catttcttcc acgtcgccgt cgaaatgctg gatttcaacc gtaccttcct cgtcgtcgat
     2281 ggcggtcaca tagaactgct gccccttgtc cagatgccgg taccattgcg atacctgggg
     2341 ttcaagcgtt gtggtcatga aacacctcct gctttgccgc cggccttccg tgtggataat
     2401 ttagtttcat tcaggtatag ccggcattga cccctgtcaa agatacggac aaaaaacacg
     2461 atgtagagtg attccagcaa ggaattcagc ggggacgaga gcgatgaacc aggatgcgcc
     2521 agtccttttt gcccttcagg ccagccgtgc attcggcgaa gacgtggcgc ggcatctcgg
     2581 catcgcatta agcgcgcacg aggaaaggga attcgaggac ggcgaacaca aatcccgccc
     2641 gcgggtcaat gtccgtgaca aggacgtttt tgtcattcaa tcgctttatg gggatgcgca
     2701 gcagagcgtg aatgacaaac tggtgcggct gctgtttttc atcggcgcgg ttaaagatgc
     2761 ttctgcggcg cgtataaccg ccgtggttcc ttatctcgcc tacgcccgga aagacgcaaa
     2821 aacccagacg cgcgatccgg tcaccacgcg ctatgtggcg cagttgttcg aggcggcggg
     2881 caccgaccgc gtggtcacct tggacgtgca caatctcgcg gcctttcaga atgccttccg
     2941 catccgcacc gaccatctgg aggcgacgaa attgttcgtg gcgcacgtga cgccgctgtt
     3001 gcagagcgaa ccccacatca ccgtggtgtc accggatatc ggcggtataa aaagggccga
     3061 gcgtttccgt caggcgctgg cgcgtgcctt gggccgggaa ctgaatctgg ccttcatgga
     3121 gaaggcgcgg gcgaaaggcg tgatgcgcgc cggccggctg gcgggagagg ttgtgggcac
     3181 cgcgatcatc atcgacgacc tcatcagcac cggcggaacg ctgctgcaag ccgcgatcgc
     3241 ctgccgcgaa cgtggcgcga ccaaagtcta tgccgccgcc acgcacggcc ttttcatcgg
     3301 ggacgcaggc tacctcttgt ccggaaacga gctcgacaag atagtcatca ccgacacggt
     3361 cccgccgttt cgtctgaacc gccagctgat cgccaagaaa ctcgctgtcg tttccgccgc
     3421 gccgctgttc gcggaagcga tccggcgcat ccacaccggt ggttcgttgg tggagttgct
     3481 ggaggcataa ccctttcgta ggttgggctg agcaacgagc caatggggtc agattcgatt
     3541 gagcaagtaa accaacgaat ttcaaacctc atgattcatt caatcgagtc tgaccccatt
     3601 ggtccttttg ttaggggccc ctaaaatttc ccgctgtaac tttcggcgag ccgcagatat
     3661 tccccggcca ccccgcgcca gcgtggccag gcgctttgcg aaagatcgtg cacatgccag
     3721 atggcgagcc gggcacgcag gcaggtgcga tacgctttgt aaaaatacac aagttgcggc
     3781 ggcggataat cgccggtgaa acgacggtaa gcgccgaaca gctccggctc cacctgcggc
     3841 gcgcccagac gctcacattc catggcgaga aacgccagtt catccgcggg atcgatgagg
     3901 cggaaagagc ggttgaattc caggcgatcg aaaatcaccg gcagcgattc cacgcagaca
     3961 tgctccggtc gcagatcgcc gtgcccttcg ataatcctgc cgtcagcggc gcggcgttcg
     4021 aacagttctg gcgctgcatg cagaaattca atctgggttt catgcacggc cgtgaccagc
     4081 gcagcggaca ggccatacgc cggatcggac agcgcatcgt gattgatctg caccttgtgc
     4141 tcgtattgcc ggcgatattc ctcaccgctg atttccagcg cggcgctttc gcggtaaaag
     4201 cgggccagca cctccgccac cgcgtgcgta tccatgggct ctagcacccg cttccggatc
     4261 aggtaatcca gcatccgttc cgccggcagg cgacgcatca tcacgagcca gtcaaggatc
     4321 tcgccctgcc cgtcgaggcg catctctccg tcactgtcca gcgccagcgg aacgacgtcg
     4381 agatagacgt tgggcgcgag gcgctggttc agccgcagtt cttcttcgca gttcattttt
     4441 cgcgccgcca ctgtgctgaa atccagatag tcgtaatgca ccggtttctt gagcttgaag
     4501 gcatgccgcg gcgtcagaaa aacccacgac atgtgggttt cgatggtttc gacgtgcgcc
     4561 ggcttttcgg gataggcccg cggctgccgc agaaacgcga tcctggcttt ctgatccgga
     4621 acggtatcct gccgcgacat cagtttcatg attccatgcc ctttccggtc aggtggcgct
     4681 cgaaccagcg ggccgcggcg gtggcgacct gctcgagcgt tccgggttct tcgaacaaat
     4741 gcgtggcgcc gggaatgatt tttaattcct tctcgccacg cagttcatgg tatgccactc
     4801 gattgagtcc aatcacgacc gtgtcgtctc cacccacgat cagcagggtc ggcgcctgca
     4861 cgcgcgggag atcgtcgagc gctaggtcgg gacggccgcc gcgtgaaacc acggcgcgga
     4921 cttccggccc caaagcggcc gctgccatga gtgctgccgc cgcgccggtg ctggcaccga
     4981 aataaccgag cgccaagccg cgcgtggccg tctgttgtcc ggcccattgc gtggcaagca
     5041 gcaggcgccg tgtgagcaga tcgatattaa aacgattctc gtagatccgg tcttcctttt
     5101 ccgttagcag atcgaacaac aacgtcgcca cgccgctctc ccgcagcacg ccggcgacat
     5161 agttgttgcg cggactcagg cggccgctgc cgctgccatg cgcgaacagc acgaggcctt
     5221 tcgcggctgg cggtatggcg agcgcgccct gatggatggc ggaatccatc ggaatactga
     5281 cggtctgctc gcgctcacgt gtggccatgt ttcctcgctt accccgccgg agtcatcgat
     5341 cgcagcagcg cgatcacttc ttcatcggtt acctggagga aatgatcgta ataggcgccg
     5401 actgcgccga gataataagg agaaggatcc agcaccacga actcgtccac ctcgcgtgac
     5461 aaccgctcca cggtatcagg tggcgcgacc ggtaccgcga ccaccagatg cgccggctta
     5521 cggcgccggg catcgcgaat ggaaacctcc atggtaaggc ccgtggcaat gccgtcgtcc
     5581 acgatgatcg ccgtcttgcc ttccaccggc gatggctcgc gtttgcccag gtacagctcg
     5641 cgacggcggc gcgcctcctg gcgctcggcc tcgacttcct gctgaaacca ccggggatcc
     5701 acgcgcgcga cttcctgctg gttgcacacc atctcgccgt tctccacgac ggcacagatg
     5761 gcgtattcgg gttgccgggg gtgaccgatt ttccggggga taaggagatc gagcggcatg
     5821 cgtaacgcgc gcgccacttc cacgcccagc accacgccgc cgcgtggcag ggcgtacacc
     5881 acgccatggc gtttctggta ttttttcagc gcctgcgcga gctgcttgcc cgcatcgacg
     5941 cggtcccgga agtgcatggc aaagctcctc gctctgccgt gaatgagaaa tcagattatt
     6001 cctttccggc gagccgtcct tgataagaat caatatcgcg cggcggcgcg cctaggggat
     6061 ggattcggtg gtgtaacgct gcactcggat gcggctcgac caatagtcgg gaggcaggcc
     6121 ggccttgtgc ttgagctggc gcaggaattc gcgcggatcg ggcagctgtt cccacaccga
     6181 gggcagaaac gtgccgcgat tataaccctc ctcgatgatc aagccatcca cgcgcggacg
     6241 caactgcgcc agcagttcat cttccgagga aaactgcaac ggctcgggca gactcagcac
     6301 ggaaatgtgt atatcgaggc gctcgtactc cgaccaggtg agcgctgaaa aacgcgggtc
     6361 gcggaacgcc gcactccagg cgttgctcac cacgtcttca acgagtgtcc ggcgcgcctc
     6421 gagcgtgccg atgcagccac gcaactgcgc ctccaggtgc agcgtaacaa acgtggcgcg
     6481 cagaacacgc aaggattccg gatagtcgct cgcccggaca ctgagggcct cgccgcacag
     6541 gcctttcttg atggattcac cggccagctg caataactgc tgcttgtgcg gctgtgcgag
     6601 cgtggaagaa atgttattca aagacgtaag cgccataacc tactacccgg tcatgcgaac
     6661 cggcggtgtc accggaattg cgcagatcaa tggtcctggc cttgaggccc cgtttgcgcg
     6721 ccacctgcag caggccgttc accgggttgc gcccgcaggc atgatcgtat tcgatggcct
     6781 ccggccgcag ttgctcgatc gcctgcgagg ttgcgcgatc cagcctctgc gcggttttgt
     6841 agtcgtggta gtggctcagg tccgagctga tcacgatcag cgtttccggc ccgccccaga
     6901 gcgtatcgag cacctcggcc acctctccgg gttcggcatc gcccaccacc agcggcacca
     6961 ggctgaactc gcccaacgct tcctgaagaa acggcagatg cacctcaagg ctgtgctcct
     7021 gcgcgtgagc ggcgtccatt acttgaacct gcgggagacg ggaaattttt ttgaccgcct
     7081 cctggtccac ggtaatgcgt cccagcgggg tctgaaagca atccgcgctg gagagcgcga
     7141 gcccatgaaa gccgacgcga tgcgcggggc cgagcagcac cacgcgtgtg atacgcccac
     7201 gcgccggccg gatgcgcgca taagccgagg ccgcgatggg gccggaataa atataacccg
     7261 catggggcgc gataatggcc ttgggcacgg cgccggccgc ggccacggcg ccgagaaaac
     7321 ccgtgaccat tgcttgcaat tcacgcggat catcgggata aaacaggccg gccactgccg
     7381 ggtttcgtac gctcgtcatg acgtttcctg ccagaaagta gctataacaa tgtgcccgcc
     7441 cggtgcgatt ttcaagtaaa accgcgttgg cgtagtatga atattgattt gacggccctg
     7501 tggccgcatt tctactctca agttcgtaac tgcgccatcc ttggcgcact tctaccagcc
     7561 atggaaacgg aaattctcga ttcggcggcg gtggtcccga cccgctactg gcacaagctc
     7621 gacgacgggc gcgtgcagtg tgacgtgtgc ccgcgctact gcaagctgca cgagggccag
     7681 cgtggcctgt gcttcgtgcg cggcaaccat aacggccaga ttgtgctgac cacttatggc
     7741 cggtcgagcg gttattgcgt cgaccccatc gagaagaaac cgctcaatca tttcctgccc
     7801 ggcacgccga tcctgtcctt cggcaccgcc ggctgcaatc tcgcctgcaa gttctgccag
     7861 aactgggaca tcagcaaatc gcgcgaactc gacacgctga tggatcaggc ctcgcccgag
     7921 accatcgccc gcgccgcgaa ggcgctcgaa tgccgcagcg tggcctacac ctacaacgac
     7981 ccggtgatct tcctggaata cgccattgac gtggccaagg cctgtcgcga ggtcggcgtc
     8041 aaatccgtag cggtcacggc aggttacatg tgcgaggagc cgcgacgcga gttctatcag
     8101 tacatggatg cggccaacgt ggacctgaag gccttcaccg aagaatttta ctggaaggtg
     8161 accggcgcgc atctgctacc cgtgctcgac accctcaaat acctgaagca tgaaaccaag
     8221 gtgtggtttg agctcaccac cctgctcatt cccggcctca acgactcgga gaaagagatc
     8281 gaggaaatga cgcagtgggt ggtggaaaat ctcgggcccg acgtgcccat gcatttcacc
     8341 gcgtttcatc cggactggaa aatgcgcgac atcccggcca cgcccgtggc gacactcatc
     8401 cgttcgcgca aaatcgccat gaaaaatggc gtgcgctatg cctacaccgg taacgtgcac
     8461 aacgaagaag gcgattcgac gtattgtcat cattgcggca ccaaactcat cgggcgcgac
     8521 tggtacaaca tgaccgcgtg gaatctcgat gcccaaggca agtgcaaggc ctgcggcact
     8581 gcctgcgccg gcgtgttcga ggccgagccc ggcaattggg gtgcacgacg tctgccggta
     8641 cgcctggcca atttcgctgc ctgatttaat tcctgccgtc ttattcaccc gaattcccgg
     8701 tttgacggct gtcatgtttc cgcggggata acggtgttat acatattttc atgccggacg
     8761 ccgcaaacag ggtttttgat cttcctgaaa tgcgcaaccg ggggcgggtg ttccgtgacc
     8821 ggacgcaggc tggagcagtg ctggccggca tgcttggaga gttccgcggg tcagacacgc
     8881 tgatactcgc aataccggct ggcggcgtgc cggtagcggc ggagattgcg aagcaactcg
     8941 gcttgacgct cgatctggca gtggtcagca aaatcacact gccgtggaac acggaatcgg
     9001 gatacggcgc cgtggccttc gatggcacgg tgcgactcaa ccagaaactg atcgcagcat
     9061 tgggcctggc ggaaacggca gtgcaggaag gcgtggcgca aacgaaggaa aaggtggcgc
     9121 gacgcgtgaa gctattgtgc agagaccgcc gccttctcgg gctgacggga cgcagcgtca
     9181 ttctggttga tgatggactg gcttccagct ttacgatgca caccgccatc gatgccttgc
     9241 atcgacaggc ggcggacaag atcatcgtcg ccgtaccgac cgggcacgcc gaagccgtgg
     9301 cacgcattgc tgcctcggtc gattcactgt actgtcccaa catccgccag ggaatgagtt
     9361 ttgccgtggc ggatgcatac gaacagtgga cagatgtgag cgaggcaacg gtcgcggcga
     9421 tcatgcaatc attttccggc acgcaacacg cggtatgagc gccatgcgct gggaacattt
     9481 tccacacgag gccgacatgg gcatacgcgg catcggccac acccgcgacg aggccttcga
     9541 gcaggcggcg ctggccatga ccgcggtcat caccgacccg gcgcaggtca aggccgcgga
     9601 aatggtggaa atcacgcggc aggcgccgga cgacgaattg ctgctggtgg actggctcaa
     9661 tgccctggtt tatgagatgg caacgcgcaa gctgcttttc agccgcttcg aggtccacgt
     9721 gaaggatcat gtgctcactg cccgcgcctt cggtgaaccg atggacacct caaaacatca
     9781 tatggcggtc gagatcaagg gcgcgaccta cacggcactg cgggtcgcgc aggagagcaa
     9841 cggcgaatgg gttgcccaat gtatcgttga cgtctaaccg ggaatgtcac gatggatatt
     9901 gcactctttt cccgtcacac cgattacgag tgggaaatcg ctccgcacgg cgccatgcgc
     9961 gtgcctgcgg taatttatgc cgacgaagcg ctgatccgtg ccatggatca caaggtgtac
    10021 gaacaggtct gcaatgtcgc caccctgccc ggcatcgtga aggcgtccta cgccatgccc
    10081 gatgcgcatt ggggttacgg ctttcccatc ggcggcgtgg ccgccttcga tcccgacgaa
    10141 ggcggcgtgg tgtccgcggg tggcgtgggc ttcgacatct cctgcggcgt gcgctgcctt
    10201 cacaccggac tgaacagcga ggatgtcatg gcggtccaga aatccctggc cgatacctta
    10261 tatagggaaa ttccggcggg tctgggcagc accggcacca tccgcctgaa tcagtccgaa
    10321 atggacgcga tgctggcggg cggcgcggct tgggccatca aacagggcta cggcagcgcc
    10381 gccgatctcg atcacatcga agagcgcggc cagatgcgcg gcgcgcgacc ggattgcgtg
    10441 tccgaccacg cgaagaaacg ccagcgtgac gagatgggca cgctcggctc gggcaatcat
    10501 taccttgagg tacagaaagt cgcgcaggtt ttcgacacaa acacagcaaa tgtgtttggc
    10561 atccgcgaaa acgacgtagt ggtcagcatt cactgcgggt cgcgcgggct cgggcatcag
    10621 atcggcaccg aattcctgaa agaaatggtg atcgccgcgc cgcgctatgg catcaccctg
    10681 cccgaccgcg agcttgcctg cgcgcccatc aactccccca ttggtgagga ttatctgggc
    10741 gccatgcgtg ccgggatcaa ctgcgcgctc gccaaccgcc agattctcac tcatctcgtg
    10801 cgcgaagcct tctcgcaagt catcccgaag gctcgtctac cgctgcttta cgacgtttct
    10861 cacaatacct gcaaggtgga agaacatgtc gtggacggca aacggcgcaa gctttacgtg
    10921 caccgcaagg gcgccacgcg cgccttcggt ccgggccatc ctgatagtcc cgatatgctg
    10981 cgcccagtcg gtcagccggt attgatcggc ggttcgatgg gcaccagctc ctatattctc
    11041 gtcggcacga aaaaaggcga ggcgctgtcg ttcagctccg cctgccacgg cgccggtcgc
    11101 gccatgagcc ggcatcaggc ctacaaaacc tggaggggcc ggcaggtgat cgacgagctg
    11161 gccggccgcg gaatcctgat ccgtacgcgt tcgtaccggg gcgttgccga agaagcaccg
    11221 ggcgcgtaca aggatgtcag cgcggtggtt gaagccacgc acgccgcgaa gcttgcgcga
    11281 aaggtggcaa aactggtacc gctggtctgc gtcaaagggt gattttccaa ggcttggaaa
    11341 atcgggtact atttt
//
LOCUS       DBNGZE010000002         2959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1319873, whole genome shotgun sequence.
ACCESSION   DBNGZE010000002 DBNGZE010000000
VERSION     DBNGZE010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1319873"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..602
                     /locus_tag="AC16FV_00070"
     CDS             162..602
                     /locus_tag="AC16FV_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2848489.1"
                     /translation="MAGRSPVKASDEQRSALEVLAVSRNRGEADRARAILLTLDGWTS
                     ARIAEVFRVREDTVRFWRGDFARGGVEAIKASVYTGLPPVKTEAALRVCAPLLAAPVA
                     NRANWTIPRLIAEIKKRELVDIGPSQLSKALRKKTSGGGGRGAR"
     gene            614..1219
                     /locus_tag="AC16FV_00075"
     CDS             614..1219
                     /locus_tag="AC16FV_00075"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR2848490.1"
                     /translation="MAAEIERVGLRLQLRKAQAEAGDIVLLYGDESEALTHPYLARAW
                     AERGADLRVPAPGQAKKAAMMGSLDHVTRRLIVHTSRTKRSADFLAHLEQLDRLFGPK
                     PGRATRPVVLVEDNGPIHVSKITLAALDARKHWLTIEWLPKYTPELNDIEVVWRDLKA
                     HYLAHQTFTDAKALDREIHRAVEALNNERNVDSLVNWRIYA"
     gene            1293..2675
                     /locus_tag="AC16FV_00080"
     CDS             1293..2675
                     /locus_tag="AC16FV_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR2848491.1"
                     /translation="MLTDCNTSPLGFEGLGSRKVTASFDGGAITSNAGALLLRQTDRV
                     IGLSRQAAACFRDARSQEDIEHTLETLVAQRVHGIALGYEDLNDHDTLRRDPVLGLIS
                     GKLEARRSNCAVLAGKSTLNRLEHAPKADGDRYRKISVDGEAMKELFVSLFIQAKKTA
                     PRRLILDLDATDDPVHGNQEDRFFHGYYKCYCYLPLYIFCGRELLLAKLRPANIDASA
                     GSKEEIAWIVARLRQKWPDLEIWLRADSGFCREDLMAWCEANGVHYVFGLARNARLEA
                     EIEGEQAEAEQRAAKSGKAERIFKEFQYKTRQSWSCERRVVAKAEHLAKGPNPRFIVT
                     SLTSKMAAARELYEKIYCARGEMENRIKECQLDLMADRTSTATLRANQLRLWFSSLAY
                     VLVTALRRLALEGTELENATAGTIRLKLLKLGALVTVSVRRIKIAIASACPLRHVFAT
                     AQARLYAAAA"
BASE COUNT          629 a          886 c          906 g          538 t
ORIGIN      
        1 cgcgaataaa agaagggcat agatgccggg cttcaacatt gtttcctccc tcaaagaatg
       61 atgcgcgaca ggcggcatct ccatactaag cagactcctg gaacttgacc aacgaatctc
      121 gactgtgatt ccgtcttcgt attgattcgc ggaggcggga catggcgggc agatctcctg
      181 ttaaggcgag tgacgagcag cggtcggcgc tcgaagtttt ggcggtttcg cgcaatcgcg
      241 gcgaggcgga tagggcgcgc gccatattgc tgacgctcga tggctggacc agcgcgcgga
      301 tcgccgaggt gttccgcgtg cgcgaggata cagttcgttt ttggcgcggc gatttcgcgc
      361 gcggcggcgt cgaggccatc aaggcgagtg tgtatacagg cctgccgcca gtgaagaccg
      421 aggctgcgct gcgggtttgc gcgccgttgc tcgcagcgcc cgtcgcgaac cgtgcgaact
      481 ggaccatccc gcgcctcatc gccgagatca aaaagcgcga actcgtcgat atcgggccat
      541 cgcaactctc caaggcgctg cgaaaaaaaa cttccggtgg cggcggccgc ggcgcacgct
      601 gaaggggcgg caaatcgcgg ctgagatcga gcgcgtcggg ctacggctcc aactgcgcaa
      661 ggcccaggcc gaggccggcg atatcgttct cctctatggc gacgagagcg aggcgctgac
      721 gcatccttat ctggcgcgtg cgtgggccga acgcggcgcc gatctgcgcg tgccggcgcc
      781 gggtcaagcc aagaaagcag ccatgatggg ctcgcttgat cacgtcaccc gccggctcat
      841 cgtgcatacc agccggacca agcgcagcgc cgatttcctt gcgcatctgg agcaactcga
      901 tcgcctcttt ggtcccaagc ccgggcgcgc gacaagaccc gtcgtgctgg tcgaggataa
      961 tgggccgatc catgtcagca agatcaccct ggcggcgctc gatgcgcgca agcactggct
     1021 caccatcgag tggctgccca aatacacgcc tgagctcaac gacatcgagg ttgtctggcg
     1081 cgatctcaag gcgcattatc tggcacatca gacatttacc gacgccaaag ccctcgaccg
     1141 cgaaatccac agggcggtgg aagccttgaa caacgagaga aatgtcgatt cgttggtcaa
     1201 ctggcgaatc tatgcttagg tacatcggct gagccgaatc agagaaagtg gtcttgcaac
     1261 agagacttac cctgattccg gaggcgcagc cgatgctaac agactgtaat accagcccgc
     1321 tgggatttga aggcttggga agccgcaaag tcacagcgtc gttcgatggc ggagcgatca
     1381 cctcgaatgc gggtgcgctg ctgttgcggc aaacggaccg cgtcatcggc ctgtcccggc
     1441 aggctgcggc ctgtttccgc gacgcccgca gccaggagga tatcgagcac acgctcgaaa
     1501 cacttgtggc ccagcgtgtc cacggcattg cgcttggcta tgaggacctg aacgatcacg
     1561 atacgctgcg ccgcgatccg gtgctgggcc tcatctccgg taagctcgaa gcccgccgct
     1621 ccaattgcgc cgtgctcgcg ggcaagtcca cgctcaaccg gctggagcat gcgccaaagg
     1681 cggacgggga tcgctaccgt aaaatcagtg tggacgggga ggccatgaaa gagctgttcg
     1741 tcagcctttt cattcaggcc aaaaagacag cgccccgaag gctgatcctc gatctcgatg
     1801 ccacggacga tccggtccac ggcaaccagg aggaccgctt cttccacggt tactataagt
     1861 gctactgcta tctgccgctt tacattttct gcggccgcga gttgctgctc gccaagctcc
     1921 ggccagcaaa catcgatgct tcggccggct ccaaggagga aatcgcctgg atcgtagccc
     1981 ggctcaggca gaaatggccg gatcttgaaa tctggctcag ggcggatagc ggcttctgcc
     2041 gggaagactt gatggcttgg tgcgaggcga atggggtgca ttacgtcttc ggcttggcgc
     2101 gcaatgcgag gcttgaggct gagatcgagg gcgaacaggc agaggctgag caaagggctg
     2161 caaaaagcgg caaggccgag cggattttca aggagttcca atacaaaacc cgccaaagct
     2221 ggagctgcga acgccgcgtt gtggccaagg cggagcacct ggccaagggg cccaatccgc
     2281 gctttatcgt gacctcactg acaagcaaga tggctgctgc gcgagagctt tacgagaaaa
     2341 tctattgcgc ccgcggcgag atggaaaacc gcatcaagga atgccagctc gatctcatgg
     2401 cggaccgcac ctccaccgca acgctcaggg caaatcagct ccggctgtgg ttctcctcgc
     2461 ttgcctatgt gctggtaaca gcactgcgcc gccttgccct tgaggggaca gaacttgaga
     2521 acgccactgc cggaaccatc cggctgaagc tcctgaagct tggcgcgctg gttacggtca
     2581 gcgtaaggcg catcaagatc gccatcgcct cagcctgtcc gctgcggcat gtctttgcaa
     2641 cggcacaggc ccgattatac gcagccgccg cctgatgcgg ccggcagccc ctacgccagc
     2701 aacaacaaac atcgctggac tgaaccaaca ctgctatatg cggtgcataa tcgccatgcc
     2761 tgctgagcgc gacccagcac ctaaaataga tagatctaac ctaacgccgc actgtcgggc
     2821 attccatgag aaatgcgggt tagcgatggc aaccgccggc tcatcagcgt caccgaaatc
     2881 tccggaatgg agagtgatgt catccagatt caggagctgt tccgttttgt ccggcatggc
     2941 attgcccccg atggacaga
//
LOCUS       DBNGZE010000003         3872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1181031, whole genome shotgun sequence.
ACCESSION   DBNGZE010000003 DBNGZE010000000
VERSION     DBNGZE010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1181031"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..645)
                     /locus_tag="AC16FV_00085"
     CDS             complement(88..645)
                     /locus_tag="AC16FV_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR2848492.1"
                     /translation="MNTFFTEASVLIVEVVFGLYILAVLLRFLFQLVRADFYNPISQF
                     LVALTNPLLKPLRRVIPGLFGIDIASLVLLLTLQLLELYVLSWLKDLPVTTLPLVLAS
                     LVSLVRLTINVYFYAILLRVILSWFMPYGMHQNPAGNLLASLTEPLMRPARRLIPAIG
                     GLDLSPIIVLVGLQLLQLALTHLLR"
     gene            complement(642..1493)
                     /gene="proC"
                     /locus_tag="AC16FV_00090"
     CDS             complement(642..1493)
                     /gene="proC"
                     /locus_tag="AC16FV_00090"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR2848493.1"
                     /translation="MVTTSAHGAHPLIGFIGAGNMARSLASGLLKNGWGKNQIILSDP
                     ESTQRQAIESVLGVKTFTDNHVVATQADILVLAVKPQVLAEVAKTLTAAVQKKQPLVI
                     SIAAGVRLEDLDRWLGGGLAIVRAMPNTAALIGSGASGLYANERVNESMRNQAESILR
                     AVGVTVWLEDEYLMDVVTALSGSGPAYFFLVMEALEQAAIESGLDPKQARLLTLETAF
                     GAAKMALEGHEEPSQLRRRVTSPGGTTEQAVKVLEQGDIRHLFKKAVQAAVNRGREIA
                     DMFGKKA"
     gene            1693..2769
                     /locus_tag="AC16FV_00095"
     CDS             1693..2769
                     /locus_tag="AC16FV_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR2848494.1"
                     /translation="MDIAELLAFAFKQNASDLHLSAGLPPLIRVDGDIKRINVDPLDD
                     RTVHNMVYDIMSDKQQKDYEEFLETDFSFELPNIARFRVNAFNQNRGPAAVFRTIPSK
                     VLTLEQLNCPPVFKQIADQPRGIVLVTGPTGSGKSTTLAAMVNHINETEHGHILTIED
                     PIEFVHTSKNCLINQREIGKDTLGFNNALRSALREDPDVILVGEMRDLETIRLALTGA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAAEKDMVRAMLSESLRAVISQTLLKKIGGSR
                     VAAHEIMIGTPAIRNLIRENKVAQMYSSIQTGQNVGMQTLDQCLQDLVRTRQIHVDDA
                     RTVAANKDLFNSASAALAAGGAKK"
     gene            2766..>3872
                     /locus_tag="AC16FV_00100"
     CDS             2766..>3872
                     /locus_tag="AC16FV_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR2848495.1"
                     /translation="MNFIDLLKLMKHKKASDLFVTAGVPPSIKVDGKLMPVTKQALTP
                     EQSRAFAYGIMNEDQRRQFEANNECNFAIAPQEIGRFRVNVFMQQQRVGMVLRTINTD
                     IPRFDDLHLPKVLADLALTKRGLILFVGGTGSGKSTSLAAMIGYRNEYSYGHILTIED
                     PVEYVHDHKNCIVNQREVGVDTASYEVALKNAMRQAPDVILIGEIRARETMDLAIQFS
                     ETGHLCLSTLHANNANQALDRIINFFPEDKRNQLLMDLGLNLKAIVSQRLIPMKSGKG
                     RVPAVEVMINSPLIADLIMEGKIPEIKDLMGKSTEAGMQTFDQSLFNLYEADLISYDD
                     ALRNADSVNDVRLRIKLEGKDSKSRELGESMRNVT"
BASE COUNT          829 a         1176 c         1176 g          691 t
ORIGIN      
        1 acgccggcaa tggaatccgc gctggcgcgt ggctggaccc gtatctggag aatgaggtcg
       61 gaaccgtccc ggcggaacca gcccacgcta gcgcagcaga tgggtgagcg cgagctgcag
      121 cagttgcagg cccacaagca cgatgatcgg cgaaaggtcg agtccgccga tggccgggat
      181 caggcggcgc gccgggcgca tcagcggttc cgtaagactt gccagcaagt tgcccgcggg
      241 attctggtgc atgccgtagg gcatgaacca gctcaggatc acgcgcaaca ggatagcgta
      301 gaaatagacg ttgatggtga gtcgcacgag agagaccagg gaggccagca ccagcggcag
      361 cgtggtgaca ggaagatctt tcagccacga caacacatag agctccagta gttggagcgt
      421 caggagcaat accagcgagg cgatatcgat gccgaacagg ccgggaatga cgcgtcgcaa
      481 cggtttgagc agcgggttgg tgagcgccac cagaaattgg gaaatggggt tgtagaaatc
      541 ggcgcgtacc agctggaaca gaaaacgcag cagcactgcc aggatgtaca gtccgaagac
      601 gacctcgacg atgaggacgc tcgcctcggt gaaaaacgta ttcatgcctt tttcccgaac
      661 atgtcggcaa tttcacggcc gcggttgacc gcagcttgca cggccttctt gaacagatga
      721 cggatatcgc cctgctccag aaccttcacc gcctgttccg tcgtgccgcc gggcgaggtc
      781 acgcgtcgac gcaattgtga tggctcctcg tgaccttcga gcgccatctt ggccgcgccg
      841 aaggccgtct cgagcgtgag caggcgcgcc tgttttggat ccagcccgga ttcgatcgcg
      901 gcctgttcga gtgcttccat gacaaggaag aaatacgccg gtccgctgcc ggacaacgcg
      961 gtcaccacgt ccatgagata ttcgtcctcg agccataccg tcacgcccac ggcgcgcagg
     1021 atggattcgg cctgattgcg catggattcg ttgacacgtt cattggcgta cagtcccgag
     1081 gcgccgctgc cgatgagcgc ggcggtgttg ggcatggcgc gcacaattgc gagtccaccc
     1141 cccagccagc ggtcgagatc ctcaaggcgc acgcccgcgg cgatggaaat gacgagaggt
     1201 tgtttcttct gaacggcagc agtcagagtc ttggccactt ctgccagaac ctgtggcttg
     1261 accgccagca ccaggatatc ggcctgggtg gcgacgacgt gattgtcggt aaaggttttt
     1321 acacccagaa cggattcgat ggcctgtctt tgtgtcgact cagggtcgga aaggatgatc
     1381 tggtttttgc cccagccgtt tttcagcagt ccggaggcca ggctgcgcgc catgttgccg
     1441 gcgccgatga atccaatcag agggtgtgcg ccgtgtgcgg aggtggtgac catggctgcc
     1501 aaagtttaaa acgccgtccc gtggaacgga agccgaatcg tgggcaccag cccgtggaaa
     1561 gtctggcatt ttcgcggatt tcaccgaccg gcggcgatgg tatccattgc aggccggccg
     1621 ggaatggact agaaattatg gcaagggccg aatttataac gcttttgcca tccacggccc
     1681 acggagaatc tgatggatat cgccgaactg ctggcgtttg ccttcaaaca gaacgcctcg
     1741 gacctgcatt tgtcggccgg cctgccgccg ttgatccgcg tggacgggga catcaagcgc
     1801 atcaacgtgg atccgctgga cgaccgcacc gtccacaaca tggtctacga catcatgtcg
     1861 gacaagcagc agaaggacta cgaggaattc ctcgagaccg acttctcctt cgagttgccc
     1921 aatatcgcgc gtttccgcgt caacgctttc aaccagaacc gcggtccggc ggcggtgttc
     1981 cggaccattc cctccaaggt cttgacgctt gagcagctca attgcccgcc ggtgttcaag
     2041 cagatcgccg accagccgcg tgggatcgtg ctcgtgaccg gtccgaccgg ttccggcaag
     2101 tccacgaccc tggccgccat ggtcaatcac atcaacgaaa ccgaacacgg gcacatcctc
     2161 accatcgagg atccgatcga attcgtgcac accagcaaga actgcctcat caaccagcgc
     2221 gagatcggca aggacacgct cggattcaac aatgccctgc gctccgccct gcgcgaagat
     2281 ccggacgtga ttctggtggg cgaaatgcgc gacctggaaa caatccgtct ggcgctgacc
     2341 ggcgccgaga ccggacacct ggtgttcgga accctgcaca cgagttccgc ggccaagacc
     2401 atcgaccgca tcatcgacgt gttcccggcg gcggagaagg acatggtgcg cgcgatgctt
     2461 tccgagtccc tgcgcgcggt tatctcgcag accctgctca agaagatcgg cggcagccgc
     2521 gtggcggcgc acgagatcat gatcggcacg ccggcgatcc gcaacctgat ccgcgaaaat
     2581 aaagtcgcgc agatgtattc gtccattcag accggccaga acgtcggcat gcagacgctc
     2641 gaccagtgcc tgcaggatct ggtgcgtacg cgccagatac acgtggatga cgctcgtact
     2701 gtggcggcca ataaggacct gttcaattcc gcctcggcgg cgctggcggc aggtggggcc
     2761 aagaaatgaa tttcatcgac ctcctcaagc tcatgaagca caagaaggcc tcggacctgt
     2821 tcgtcacggc cggggtaccg ccctccatca aggtggacgg caagctcatg ccggtcacga
     2881 aacaggcgtt gacgcccgag cagtcgcgcg ccttcgccta cggcatcatg aacgaggatc
     2941 aacgccggca gttcgaggcg aacaacgaat gcaactttgc gatcgctccg caggagatcg
     3001 ggcgcttccg cgtgaacgtc ttcatgcagc aacagcgcgt gggcatggtg ctgcgcacga
     3061 tcaacaccga catcccgcgc ttcgacgacc tgcacttgcc caaggtactg gcggacctgg
     3121 cgctgaccaa gcgcggattg atcctgttcg tcggcggcac cggctccggc aaatccacct
     3181 cgctcgcggc catgatcggc taccgcaacg agtacagcta tgggcatatc ctcaccatcg
     3241 aggacccggt ggaatacgtg catgaccaca aaaactgcat cgtgaaccag cgcgaggtcg
     3301 gcgtggatac ggcgtcctac gaggtagcgc tcaagaacgc catgcgccag gcgccggatg
     3361 tgatcctgat cggtgaaatc cgcgcgcgcg agaccatgga cctggcgatc cagttttccg
     3421 aaaccgggca cctgtgtttg tccacgttgc atgccaacaa cgccaaccag gcgctcgatc
     3481 gcatcatcaa cttcttcccc gaggacaagc gcaaccagct gctgatggac ttggggctca
     3541 acctgaaggc catcgtttcg cagcgcctga ttccgatgaa gagtggcaag gggcgtgtcc
     3601 ccgccgtgga ggtcatgatc aactcgccgc tcatcgccga cctcatcatg gaaggcaaga
     3661 ttccggagat caaggacctc atgggcaaat cgacggaagc cggcatgcag accttcgacc
     3721 agtcgctgtt caacctgtac gaggccgatc tcatttccta cgatgacgcg ctgcgcaacg
     3781 ccgactcggt caacgacgtg cgcctgcgca tcaagctcga gggcaaggat tcgaagagcc
     3841 gcgagttggg cgagtccatg cgcaacgtga cc
//
LOCUS       DBNGZE010000004         3920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_291, whole genome shotgun sequence.
ACCESSION   DBNGZE010000004 DBNGZE010000000
VERSION     DBNGZE010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_291"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..657)
                     /locus_tag="AC16FV_00105"
     CDS             complement(250..657)
                     /locus_tag="AC16FV_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR2848496.1"
                     /translation="MVATELHDSAMRLDKWLWAARFFKTRTLAAEAVAGGKVKLNGER
                     VKAAKTVRLGDALNIHIGPYEYVVRVLALSARRGPATQAALLYEESESSQAVRKVLAT
                     RLAAERQHAIHTHGRPSKKQRRQIIQFKKSRIG"
     gene            931..1329
                     /locus_tag="AC16FV_00110"
     CDS             931..1329
                     /locus_tag="AC16FV_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848497.1"
                     /translation="MEGISDIRIAGIDERRPPRIRREPYIDLCFRLTHKAPVDWCQDF
                     VDHLSKEGFPTIIDAKECLYIETWVRLPDEIIGRLQVLKKAVADCNARYIAKIEASER
                     DKDGDIDALAREAGPQGQLNRIIAALDFGD"
     gene            complement(1417..1617)
                     /locus_tag="AC16FV_00115"
     CDS             complement(1417..1617)
                     /locus_tag="AC16FV_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR2848498.1"
                     /translation="MKMHYDEKSDALYLRLDESKIIESEEVQPGIVLDYNDNKQVVGV
                     EILRVKERVPLADLKHLDFKVA"
     gene            complement(1614..1877)
                     /locus_tag="AC16FV_00120"
     CDS             complement(1614..1877)
                     /locus_tag="AC16FV_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HIR2848499.1"
                     /translation="MPEKIHFELTAHAVTVMAERQIPVVWVERVLMGPERIEPDRVDP
                     DLRHALGRIPERDDRILRVVYNKSIKPWRVVTAYFDRSMRDKL"
     gene            complement(2083..2259)
                     /locus_tag="AC16FV_00125"
     CDS             complement(2083..2259)
                     /locus_tag="AC16FV_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848500.1"
                     /translation="MLKAVIQRLLRIRRTNRLKSDLRRIQGYWNLKAKEKGVSVAKDV
                     ERYLMEISRSKKTG"
     gene            complement(2286..2717)
                     /locus_tag="AC16FV_00130"
     CDS             complement(2286..2717)
                     /locus_tag="AC16FV_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848501.1"
                     /translation="MVKLKINTLRGGRWHDKNKILLHAAFQLLVDFVEQEQPAKLIDW
                     SADARHRKAWKEIMSLYRWWKKERPARKSVLDWKNVKWPPYKTEKIPGKDLWKWIEPD
                     RKKYAAYYRLLKQSWRQEAEWAAEDQKNLHRLVDIRDHLWT"
     gene            complement(3051..3779)
                     /locus_tag="AC16FV_00135"
     CDS             complement(3051..3779)
                     /locus_tag="AC16FV_00135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_203571596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR2848502.1"
                     /translation="MTTDTRRITVGGIAVEVVRKNIKNLQLGVYPPHGRVRVAAPLVV
                     SDEAVRLAVVDKLAWIKRQKTRFGEQPRQSQREMVNGECHYFLGKRYRLRVHEQNGPT
                     RVSVRGVAMLDLFVRRSMDAEQREAALLRWYREQLKALIPPLLERWQPKLGVKVSAWG
                     IKKMKTKWGSCNAKTARLWFNLELAKKPPQCLEYIVVHELMHLLERSHNERFTAMMDK
                     FMPGWRSHRVALNRAPLAHETWGY"
BASE COUNT          815 a         1124 c         1006 g          975 t
ORIGIN      
        1 tggttagcgc tgtttttgcg ttcatcccat tcgaagcgct ggtcactcat acctagagta
       61 taactacact gtagggtatt acaaagcatc cctcactttg ggtcttaggc tcaaacccca
      121 ccccctacgc cgggcgatca gatccttctt gagcgtggta atcgcggacc gggcctcgtg
      181 agacaagaca gcggaaagat ctgaaacctt agtggcaaga ccgggatcat tccggccggt
      241 gactttcgct tatccaatcc tcgatttctt gaattgaatg atctgacgcc gctgtttctt
      301 gctggggcgt ccgtgggtat ggatggcatg ctgccgctcg gcggcaaggc gggtcgccag
      361 gactttgcgc accgcctggc tcgattcgct ctcttcataa agcaacgcgg cttgcgtcgc
      421 cgggccgcgc cgggcggaca aggccagcac gcgcacgacg tattcatagg ggccgatatg
      481 gatgttcaag gcatcgccca ggcgaacggt cttggcggct ttcacgcgct cgccattcag
      541 cttgaccttg ccgcccgcca ccgcctccgc ggccaaggtc cgggtcttga agaatctcgc
      601 cgcccaaagc catttgtcga ggcgcatggc gctgtcgtgc aattctgtcg caaccatttc
      661 cagtcttctc aaggcttcct gcaattcaaa caaggctccg gagacagctc ccggtggttc
      721 cttatgttag cgagaactgt gttcggaccc taatttacgc gcgaactgat attttctctg
      781 aatcccgccc cgcaccagtt ttgcgcgtat agtgagatag cgtcctaccc acgggccagc
      841 acgcgcaaca cttcattccc ctcccccccc ttgacggggt gagggttagg gtgggggtga
      901 cacttcactc gtgaccgagg aaccgtattt atggaaggca tcagcgatat cagaatcgcg
      961 ggcatcgacg aaaggcgccc gccgcgaatc cgcagggaac cgtacattga cctctgcttc
     1021 cgactcacgc acaaggcgcc ggttgactgg tgtcaggatt ttgtcgatca cctgtccaaa
     1081 gaaggctttc cgacaataat tgatgccaag gagtgtctgt acatcgaaac ctgggtccgg
     1141 ttgcccgacg agatcatcgg gcgcctgcaa gtcctcaaga aagccgtggc ggactgcaac
     1201 gcgcgctaca ttgccaaaat cgaggcgagc gagcgcgaca aggacggcga cattgatgcg
     1261 ctggccagag aggccggccc gcagggacaa ctgaaccgca tcatcgctgc gctggatttc
     1321 ggcgactgag gccgcacaaa tctagaaaaa caaggggctc cataagagcc cctgcaccct
     1381 gggccgaatc cacaggggga gtgattaatt tctgtttcaa gcgaccttga agtcgagatg
     1441 tttcaaatcc gctaacggca cccgctcttt cacccgcaga atttccacac ccactacctg
     1501 tttattgtcg ttatagtcga gcacaatccc cggctgtact tcttcggatt caatgatctt
     1561 tgattcatca aggcgcaggt agagcgcgtc agatttttcg tcatagtgca ttttcataac
     1621 ttgtctctca tagaccggtc aaaataagcc gtcactaccc gccatggttt gatgctctta
     1681 ttgtagacca ctcgcagtat acggtcatca cgttcgggaa tacgtccaag tgcatggcgc
     1741 aaatcaggat caaccctgtc tggttcgatc cgttccggcc ccatcaatac gcgctcaacc
     1801 caaaccacgg gtatttgccg ttcggccatt acggtaaccg catgggcggt taactcgaaa
     1861 tgaatttttt ctggcatttt tgttaccgtc gcttacgaaa tagactgttt actccacggc
     1921 ctgggccggg cagctcgtcg attctgcgcg agctgcatta tccagtcgtg aacatcctcg
     1981 gtctgatata ccggagcacc cttctcgatc gcggcatccg cggcgagtgc ctcttgcatg
     2041 aattcccgca ttcgttcttc tgttgtgcgt ttcacctcgg aatcaacctg ttttcttgct
     2101 tcttgaaatt tccattagat agcgctctac gtctttcgcg acagagaccc ccttttcttt
     2161 tgctttgagg ttccagtatc cctgtatacg gcgcagatcg gatttgagac gatttgtacg
     2221 cctgatgcga agcaagcgct ggatcacggc ttttagcatg ttatcctacg ttctggctga
     2281 cacgtctatg tccagaggtg atcgcggata tcgaccaggc gatggagatt tttctggtct
     2341 tcggccgccc actcagcctc ctgccgccag gactgcttca acagccgata gtatgccgca
     2401 tatttctttc ggtccggttc gatccatttc cacagatctt tgccgggaat tttttctgtc
     2461 ttgtaaggag gccatttcac attcttccaa tccagcacgc ttttgcgcgc gggtcgctct
     2521 ttcttccacc agcgatacag cgacatgatt tccttccagg cttttcggtg ccgggcatct
     2581 gcgctccagt caatgagctt cgccggctgc tcctgttcta cgaagtcaac cagcaattga
     2641 aatgccgcat gcaacaggat tttattcttg tcgtgccagc gaccccctcg cagggtgttg
     2701 atcttgagtt ttaccattcc ttgacccgtg gttaaggcta actataccgc gatacatgcc
     2761 gcggtcagcc ctcgatataa aggttggcag taaggtgggg cgacggcgtg agacgtgggc
     2821 gggaaaggag atggaagctg gacccgtggg tctcggggcc aacagccgaa agcatccggg
     2881 gcggcgccct gataggtagt atgaattaat catacaggct gtgtattaac tgtcaataca
     2941 cgttactccc gcgatggcgc gcgggaagca agctattccc gaggggcgga gttggttgga
     3001 acgatttgga aaattgagcc tggtcacttt aattcttctg attctccgcg ctagtatccc
     3061 caagtctcgt gtgctagcgg tgcgcgattc aatgcgactc ggtgcgatcg ccatcctggc
     3121 atgaatttgt ccatcatcgc cgtgaatcgt tcgttatggc ttcgctctaa tagatgcata
     3181 agctcatgca ccacgatgta ttccagacac tgcgggggct tcttggccag ttccaggttg
     3241 aaccacagcc gcgcggtttt agcattgcag ctaccccatt ttgtcttcat ctttttgata
     3301 ccccaagcgc tgaccttaac accaagcttc ggttgccacc tttccaacag cggcgggatc
     3361 aaagccttta actgttcccg ataccatcgc agtagagctg cttcgcgctg ctcggcatcc
     3421 atactacgac gaacgaatag atcaagcatg gcaacaccac gcacggaaac ccgggttgga
     3481 ccattctgtt cgtgcaccct gagacgatat cgcttgccga ggaaatagtg acattcgcca
     3541 ttcaccatct cgcgctgtga ctggcgcggc tgttcgccga acctggtttt ctgtctcttg
     3601 atccaggcga gcttgtcgac cactgccaag cgcacggctt cgtcgctcac taccagcggt
     3661 gctgcaaccc gcacgcgccc atggggcgga tagacaccta actgcaggtt cttaatgttc
     3721 ttacgcacca cctccacggc aatgccgccg acggtgatgc ggcgggtatc agtagtcatt
     3781 ctggttcttt gccaattcaa ggatgcgttt ggtgtccaat tctacttcat gatcgtcgct
     3841 aatgtgcaaa gaaccaccat gtttatctct ttgcttggca atgaacgcct cttcaataac
     3901 cgatcggata gcaagccgca
//
LOCUS       DBNGZE010000005         8148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1528412, whole genome shotgun sequence.
ACCESSION   DBNGZE010000005 DBNGZE010000000
VERSION     DBNGZE010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1528412"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC16FV_00140"
     CDS             complement(<1..258)
                     /locus_tag="AC16FV_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR2848503.1"
                     /translation="MKRSPESLLFIGTNGHVRAVDKRTGKDVWEVSLPDTGFEIVSLL
                     YESGVLYAASSGYVFALNPNDGSVLWKNELEGLGLEDVVLAT"
     gene            complement(328..2073)
                     /locus_tag="AC16FV_00145"
     CDS             complement(328..2073)
                     /locus_tag="AC16FV_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848504.1"
                     /translation="MPGQEVTVTGLALSLPTKRVSAWMAETDPKYAQAWLASLPLADS
                     AETAREIYQALYTLNRQELDPARRFELMELYTAPVATVTATLESYFTRAALPLTPKKR
                     QLAEFIRQLHMEMAYGYKGCLQDLEKQRMRWGKKLLRAQALMRALHYLGEVLLHSYQV
                     YMPYPPEVWREIHAIYQYAAGHDLAREVLDTPAPVAAKTTLSHEYIRILLLGLSNPYQ
                     LPQNECRQVQRFLHQWGAKAVLKENIEAAPSAGYFLIDPATDSPPIPFPRNVAFQPGQ
                     GLCLLDAVELLRSIQFFIQRLQKGDSARTLTLGIDCLDSVCLDMLQRMMRSWGQSPRR
                     QYSRIQRSGPAFVCAGIPALHFFASGQKPFVSPATTRKETSDDRVVLPAHIEEDIARE
                     VKADEDFIALDKPVVGPMPLTEAREISATLGEAFRVDRWQIKDAAPKGLQLLRHGSSH
                     TYVRVGDVVGIQQMDEFGRWSAGVVRWMKSPEADSLEMGIELLAFGVKPVAVTPTRGA
                     KAQDYQPALLLPAIEALKRPATLLLPRGVFVPGSDLLLAEENARTVRLLQRLEYTNVF
                     ELLVFADVVPPTPQG"
     gene            2272..2595
                     /gene="fdxA"
                     /locus_tag="AC16FV_00150"
     CDS             2272..2595
                     /gene="fdxA"
                     /locus_tag="AC16FV_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR2848505.1"
                     /translation="MTYVVTENCIRCKYTDCVEVCPVDCFHEGPNFLVIDPDECIDCT
                     LCVPECPVNAIFAEDDLPEDQKQFTAVNAELAKKWPVLTEKKPSPPDAAEWENKPDKL
                     KYLER"
     gene            2585..5488
                     /locus_tag="AC16FV_00155"
     CDS             2585..5488
                     /locus_tag="AC16FV_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR2848506.1"
                     /translation="MSADAPVILVPYGDDPLRHLATLLLEQHKDRLPDLSRQVVLFPH
                     GSAVPRFRRLLREQAALKNFPALLPPVTGTLISWAVRFAGGDKHRLSATAREILLLDL
                     LHDYPQWHREYGDWPLADSLLDLFDDLALHECRLSENPAGLIRQIAKTDELSPFSDEA
                     QLVHVLWNAWRERLGKNNLQDQTLQYTDGLSRSVDQLGSETKVYLAGFVDFSRAEINW
                     IRTLLAHQRLTLLVHGSGNKDPENPGMQLVNSLQAKAHVIPARDAYTDFLDRVFATED
                     GNIRQRAQKQTTSSPTSPAHGRLVLQQAADAESEARAIDIQVRRWLTQGLRDIGIVTN
                     DRKLARRVRALLERARIGLVDAGGWTLSTTSAATALARWLECVEQNFAHAPLLDLLKS
                     PFLQPGIGQPQLDRLVSLFEQSVIRSNNITAGLEHYRQGIERSQTHWREEPAETGAAL
                     IQLIDRLEGAASSLFPLTTARPRALLEFLEALHKSLENLGLSAGFEKDDAGRELLHVL
                     DEMRAATKHSALRLSWAGFHQWLRRGMEQRRFHPPMRGRGVELMSFVESRLYRFDALV
                     IAGAVREHLPGQIGAPPYFNDSARTELGLPSLARRYATLFQDFRRLLEAAPRVLVTLR
                     REHEGERLVPSPWVERIRAFHELAYGASLHDPELEWLVQQPAIVIVNRDAPLPIPVAP
                     PAAHLPPAMLPVAFTATDYQRLINCPYQFFAARGLGLAPEEEVREEIEKVDYGIHVHR
                     ILQAFHTGVLGLPGPWRGKLDDATRPDAEAMLREISQKVFARDLRRRFLARGWLYRWE
                     KCIPAYLDWEIKRAAHWRTEATEVKKERTYQEGDAQITLIGRIDRLDRGKNGYGIIDY
                     KTGSVPARDQVLLGESIQLPFYALLLEQENIAEATFLSLEDENVKEKTSLDGESLALL
                     RTAIRERLILLKRRLDAGAPLPAWGDMETCRLCEMEGLCRREMWAEPNATTSE"
     gene            5763..>8148
                     /locus_tag="AC16FV_00160"
     CDS             5763..>8148
                     /locus_tag="AC16FV_00160"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR2848507.1"
                     /translation="MIDISRATAPENNVVVHAAAGTGKTWLLTSRIVRLLLEGTEPGA
                     ILAITFTRKAAGEIHERVTERLFALAASDEDMLAKQLREIGAGTDQASRDIARGLYEK
                     HLGAIHALRTTTFHAFCQEILRRFPLEADVPPGFDLIETTAELEQAAWLALDRKASRA
                     KEGLLPEALDTLFFECGGVTGTRTALDDFLAHRSDWWAYTENEPDPVKFASAKLRHML
                     GITDDTDTLTDFVQTPAMREQIARYAELLAGHPTATNQSHIETLMRALAEPTPSQHTF
                     DRIRGVLLTDADEPRKMKRSDTLEKKLGARHADELVSLHQEIASRFLDAIAGHKRQWT
                     SRVSQAWYTCGMALLEEYQRLKMERGVLDFADLEWKTYRLLNRSRHAEWVQYKLDQRI
                     DHLLVDEFQDTNPTQWRLLLPLLQEMMAGDPERRRSVFLVGDEKQSVYRFRRADPRLF
                     HAARHWLQQHAQAHTLTQHLSWRSSPAVIRFVNLVFHRPGDADTETEGDYLLHDFQEH
                     DTHHRQLWGRAELLPLIRRQERTVEKADQAWRNPLEQPRQTDEDRRHRQEGDLIAEKI
                     RQLLGTPIADRDKIRPMTGDDIMILLRDRTHARFYEDALRRAGIPYIGTGRGAFMQSL
                     EVRDLMHLLRNLIEPYNDLALASALRSPMFAASEEDLLRLAQNPPGSWRERLERLATT
                     EAPEGDLFRAQQLLSQWKSYIGHLPVHDLLDRIYCEGNVVARYLAAAVPHLRSRVEAN
                     LNRFLELALEIDSGRYPRLSHFLAYLERQTENDDKAPAEPAWNREPRVRVLTIHA"
BASE COUNT         1703 a         2527 c         2399 g         1519 t
ORIGIN      
        1 cgtagccaga acaacgtcct caagacctag accttccaat tcgttcttcc aaagcacaga
       61 gccatcgttg gggtttaacg caaatacata gccgcttgat gcagcatata gaactccaga
      121 ctcgtaaagc aggctgacaa tttcgaagcc cgtgtcgggg agactcactt cccacacgtc
      181 cttgcccgta cgcttgtcca cggcacgaac atgaccattc gtgccaatga acaggaggga
      241 ttccggagac ctcttcatat cgctcgcatg agcgcccaat gtaatttaga tgtcactttc
      301 tggcgcattt acttgacccg gtgatatcta accttgtggg gtcggaggaa cgacgtcggc
      361 aaacaccagc agctcaaaaa cattcgtgta ttccagccgc tgcaacaggc gcacggtgcg
      421 cgcgttttct tccgccagca gcagatcgga accgggcacg aaaacaccgc gcggcagcag
      481 cagcgtggcc gggcgcttca gcgcttcgat ggccgggagc aatagcgcgg gctgataatc
      541 ctgcgccttc gcgccgcgtg tcggggtgac cgccaccggc ttgacaccga aggcaaggag
      601 ttcgataccc atttcaaggc tgtcggcctc ggggcttttc atccagcgca ccacgccggc
      661 gctccagcga ccgaactcgt ccatctgctg gatgcccacg acatcgccga cgcgcacgta
      721 ggtatgcgaa ctgccatgcc gcagcaactg caagcccttg ggcgcggcgt ccttgatctg
      781 ccagcggtcc acacggaagg cttcgccgag cgtcgctgaa atttccctgg cttccgtcaa
      841 cggcatcggg cccaccaccg gcttgtcgag cgcaataaag tcctcgtcag ctttcacctc
      901 acgcgcaatg tcttcctcaa tgtgcgccgg cagaacgacg cgatcgtccg atgtctcttt
      961 gcgcgtcgtc gccggtgaca caaaaggctt ctggccgctg gcgaagaaat gcagggcggg
     1021 aatgccggcg cacacgaagg ccgggccgct gcgctggatg cgcgaatatt gccggcgcgg
     1081 cgactggccc caggagcgca tcatgcgttg cagcatgtcg agacagacgg aatcgaggca
     1141 atcgataccg agcgtcaacg tgcgcgcgct gtcgcctttt tgcaggcgct ggatgaaaaa
     1201 ctggatcgaa cgcagcagct ccacggcgtc cagcaggcac aatccctgcc ccggctggaa
     1261 cgcgacgttg cgcggaaatg gaatcggcgg cgaatcggtc gcggggtcga tgagaaaata
     1321 tccggccgat ggcgcggcct cgatattttc cttcaacacc gccttcgcgc cccattgatg
     1381 caggaagcgt tgcacctgcc ggcattcgtt ctgcggcaac tgatagggat tgctcaaacc
     1441 cagaagcagg atgcggatat attcgtgact gagcgtggtc ttggcggcca cgggtgccgg
     1501 cgtgtcgagc acctcgcgcg ccagatcatg cccagcggca tattgataga tcgcatgtat
     1561 ctcgcgccac acttccggcg gatacggcat atatacctga tatgaatgca gcaacacctc
     1621 gccgagataa tgcagggcgc gcatcaacgc ctgcgcccgc agcaacttct tgccccagcg
     1681 catgcgttgc ttttccaggt cctgcaggca gcccttgtag ccataggcca tttccatgtg
     1741 caactggcgg atgaactccg ccaactggcg cttcttcggc gtcagcggca gcgcggcgcg
     1801 ggtgaaatag gattccagcg tggcggtaac ggtggcgacg ggagcggtat aaagctccat
     1861 cagctcgaag cggcgcgcgg gatccagttc ctggcgattg agggtataga gcgcctgata
     1921 aatttcgcgt gcggtttcgg cgctgtcggc gagcggcaag gaagcgagcc acgcttgcgc
     1981 gtacttggga tcggtctcgg ccatccaggc tgagacgcgc ttcgtcggca atgaaagggc
     2041 gagtccggtc accgtgactt cctgtccagg caccaaagta tttccgaata ctatcaagtg
     2101 attatatagc acaccccggg tgcgacgagc cggaacgatt cgttgcggcg cgacgaaatc
     2161 ccgcgctttt tatcgattta tatcgaatat aaacaagggt ttcgcttatt ccacgcaccg
     2221 acgcgcgcgt gtatcattcg gcgcgtttta aaaattcttt ggagagcgtc aatgacatac
     2281 gtagtcacgg aaaactgcat ccgctgtaaa tacacggact gcgtggaagt ctgcccggtg
     2341 gattgtttcc atgaaggccc gaatttcctg gtcattgacc ccgatgaatg catcgattgc
     2401 acgctgtgcg tgcccgagtg cccggtgaac gccattttcg cggaagacga cctgcccgag
     2461 gaccagaagc aattcaccgc cgtcaacgcc gaactggcca aaaaatggcc ggtcctgacc
     2521 gagaaaaagc cctcgccccc ggacgccgcc gagtgggaaa acaagcccga caaactcaag
     2581 taccttgagc gctgacgcac cggtcatact ggtcccttac ggagacgatc cgttacgtca
     2641 cctcgccacg ctgctgctgg agcagcataa agacaggctt ccggatttaa gccgccaggt
     2701 tgttctgttt ccgcacggta gcgcggtgcc gcgcttccgc cgcctgctgc gggaacaggc
     2761 ggcattgaaa aattttcccg ccctgctccc gcccgtcacc ggcacgctga tctcctgggc
     2821 tgtgcgcttc gccggcggcg acaaacaccg gctgagcgcc acggcgcgcg aaatcttgct
     2881 gctcgatctg ctgcacgatt acccgcagtg gcaccgtgaa tatggcgact ggccgctggc
     2941 ggacagtctg ctggatctgt tcgacgatct ggcgctccat gaatgccggc tatccgaaaa
     3001 ccccgccggc ctgatccggc aaatagcgaa gacggacgag ctttcgccat tcagcgacga
     3061 ggcgcaactg gtgcatgtgc tgtggaacgc gtggcgcgaa cgtctcggga aaaataatct
     3121 ccaggatcag acgctgcaat acacggacgg tctgtcgcga agcgtcgacc aattggggag
     3181 cgaaaccaaa gtctatctgg ccggctttgt ggatttctcg cgcgcggaaa taaactggat
     3241 cagaacgctt cttgcccacc agcgcctgac cttgctggtt catggttcag gcaacaagga
     3301 cccggaaaat ccaggcatgc aactcgtaaa ctcattgcag gcaaaagcgc atgtcatccc
     3361 cgcacgcgat gcatacacgg attttctcga ccgcgtcttc gccacggaag atggcaatat
     3421 tcggcaacgc gcgcaaaagc agacgacatc ttcaccaacc agtcccgcgc acggccgtct
     3481 ggtcctgcag caggcggcgg acgcggaatc cgaggcgcgc gccatcgata ttcaagtacg
     3541 gcgctggctc acgcagggcc tgcgtgacat cggcattgtc accaacgacc gcaagctggc
     3601 gcgccgcgtg cgcgcgcttc tggaacgcgc ccgcatcgga ctggtggacg cgggcggctg
     3661 gacactctcg actaccagtg ccgcgacggc gctggcgcgc tggcttgagt gcgtggagca
     3721 gaatttcgcc cacgctccgc tgctggattt gctgaagtca cccttcctcc aacccggtat
     3781 cgggcaaccg caactcgacc gccttgtctc cctgttcgaa cagagtgtta ttcgctcgaa
     3841 caacatcacc gccggccttg agcattaccg tcaggggatt gaacgctccc aaacccattg
     3901 gcgcgaagaa cccgccgaga ccggcgcggc gctgattcaa ctgattgacc ggctggaagg
     3961 cgcggcgtca tccttgttcc ccctgacaac agcacgaccg cgggccttgt tggaattcct
     4021 ggaagcgctg cacaaaagcc ttgaaaacct gggtttgtcg gccggctttg aaaaggacga
     4081 cgctggccgc gagctgctgc acgtgctcga tgaaatgcgc gcggcaacca agcacagcgc
     4141 cctgcgcctg tcgtgggcgg gttttcacca atggctgcgg cgcggcatgg aacagcgccg
     4201 tttccatccg ccgatgcggg gacgcggcgt ggagctgatg agcttcgtgg aatcccggct
     4261 ctaccgcttc gatgcgctcg tcatcgccgg cgcggtgcgc gaacatctgc cgggacagat
     4321 cggcgcgccg ccgtatttca atgacagcgc gcgcaccgag ctgggcctgc cctcgctcgc
     4381 gcgacgctac gccacgctgt tccaggattt ccgccgtctg ctcgaagccg cgccgcgcgt
     4441 gctggtgacc ctgcgccgtg aacacgaagg cgaacgattg gtcccgagcc cgtgggtcga
     4501 acgcatacgc gcctttcatg aacttgccta tggcgcttca ctgcacgacc ccgaactgga
     4561 atggctcgtg caacaacccg ctatcgtcat cgtcaaccgc gatgcgccgc tgccaatacc
     4621 cgtggcacca ccggccgcgc acctgccacc cgccatgctt ccggtggcgt tcacggccac
     4681 ggactaccaa cgcctgataa attgccccta tcagttcttc gccgcccgtg gactgggcct
     4741 ggcgccggaa gaggaagtgc gcgaggaaat agaaaaggtg gattacggaa tccatgtgca
     4801 ccgcatcttg caggccttcc ataccggcgt gcttggcctt cccggtcctt ggcgcggcaa
     4861 actcgatgac gcgacccgac ccgatgcgga agccatgctg cgcgagattt cgcaaaaggt
     4921 attcgcgcga gatctgcgcc ggcgtttcct ggcgcgcggt tggctctatc gttgggagaa
     4981 atgcattccg gcctatctcg actgggaaat aaaacgcgcg gcgcactggc ggaccgaggc
     5041 aacggaagtc aaaaaagaac gcacctatca ggaaggcgac gcgcaaatca ccctgatcgg
     5101 gcgcatcgat cgcctcgacc gtggcaagaa tggctatggc atcatcgatt acaaaaccgg
     5161 ctccgtaccg gcacgcgacc aggtgctttt gggagagagt attcagctgc cgttttatgc
     5221 cttgctgctg gaacaggaaa atatcgcaga ggcgacattt ctgtcactcg aagatgagaa
     5281 cgtgaaggag aagacaagtc tcgatggcga gtcgctggcg ctgctgcgga ccgccatccg
     5341 ggaacgcctg atcctgctca agcgccgcct cgatgccggc gcgccattgc ctgcgtgggg
     5401 cgacatggaa acatgcaggc tttgcgagat ggaggggctg tgtcgccggg aaatgtgggc
     5461 cgaaccaaac gcgacgacgt cagaatgaaa gataaaaaaa gaggcggcca ttcatggccg
     5521 cctccggtat atctactgat cacttgcttc cggaatgcat ccttgcatcc cttgccacat
     5581 ccctgcttac ttccaatgtc gtcagctgcg atatgtccct atccggcatc cctgcctcat
     5641 ttcaatatag gccggggaaa caaagatgca aggaaggcga gcctttcaat gcctgaaaaa
     5701 ttccttatgt ccttcatggg gttagccggg aaagccccga taaaacccgg gcgccgggcg
     5761 ccatgatcga tatttcgcgc gccaccgccc cggaaaacaa cgtcgtcgtt catgccgccg
     5821 ccggcacggg caagacctgg ctgttgacca gccgtatcgt gcgtctgctg ctcgaaggga
     5881 ccgaaccggg cgccattctt gccatcacct tcacgcgcaa ggccgccggt gaaattcacg
     5941 agcgtgtcac tgaacgcctg tttgccctcg ccgcaagtga cgaggatatg ctcgccaaac
     6001 agctcagaga gataggcgct ggcaccgatc aggcaagccg cgacatcgcg cgcggacttt
     6061 atgagaaaca tctgggcgcg attcatgccc ttcgcaccac cacgtttcac gccttctgcc
     6121 aggaaatact gcggcgcttc ccgctcgaag ccgatgtgcc gccgggtttc gatctcatcg
     6181 aaaccacggc cgaactggaa caggccgcct ggctggcgct cgacaggaag gcctcgcgtg
     6241 cgaaagaagg cctgctgccc gaggcactgg atacgttgtt ttttgaatgt ggcggcgtca
     6301 caggcacgcg cacggcactg gatgactttc tggcgcatcg cagtgactgg tgggcttaca
     6361 ccgaaaatga accagatccg gtgaaattcg cttcagcaaa gctcagacac atgctgggca
     6421 tcacggatga taccgatacg ctcacggatt ttgtacagac tcccgccatg cgtgagcaga
     6481 tcgcgcgcta cgccgagctg ctggccggtc atcctacggc aacgaatcag agtcatattg
     6541 aaacactgat gcgcgcgctg gcggaaccaa cgccgtcgca gcacacattc gaccggattc
     6601 gtggcgtact gctgaccgac gcggatgaac cgcgcaagat gaaacgttcc gatacgctgg
     6661 aaaaaaaact cggggccagg cacgccgatg agctggtcag cctgcatcag gaaatcgcct
     6721 cacgttttct ggatgcgatt gccgggcaca agcgtcaatg gaccagccgg gtttcacagg
     6781 catggtacac atgcggtatg gcgctgcttg aggaatacca gcgactcaaa atggagcgcg
     6841 gcgtgctcga cttcgccgac ctggaatgga aaacctatcg cctgctgaac cgcagccgtc
     6901 atgccgagtg ggtgcaatac aagctggacc agcgcatcga tcatttgctg gtggatgaat
     6961 tccaggacac caatccgacc cagtggcgct tgctgctgcc gctgttgcag gaaatgatgg
     7021 ccggcgatcc ggaacgtcgc cgcagcgtgt ttctcgtcgg cgacgaaaag cagtcggtct
     7081 accgtttccg ccgcgccgac ccgcgcctgt tccacgccgc gcgtcattgg ctgcaacaac
     7141 atgcgcaggc gcacaccctg acgcagcatc tgtcgtggcg ctcctcgccc gctgtcatcc
     7201 gtttcgtgaa cctcgtgttt caccgccctg gcgatgccga taccgaaact gaaggtgact
     7261 atctgctgca cgacttccag gaacatgaca cgcaccatcg gcaattgtgg ggacgggccg
     7321 agctgttgcc gctcatccgg cgccaggaac gaaccgtgga aaaggccgat caggcctggc
     7381 gaaatccgct ggaacaaccg cgccagacgg acgaagaccg gcggcaccgt caggagggcg
     7441 atctgatcgc cgaaaaaatt cgtcagcttc tggggacccc gatcgccgac agggacaaga
     7501 tacgacccat gaccggcgac gacatcatga tcctgctgcg cgatcgcacc catgcgcggt
     7561 tttacgagga tgccctgcgg cgggccggca taccttatat aggaaccggc cgcggggcct
     7621 tcatgcagtc actggaggtg cgcgatctca tgcacctgct gcgcaatctg atcgagccct
     7681 acaacgacct ggcgctggcc tcggcgctgc gctcgccgat gttcgccgcc agcgaagaag
     7741 acctgctgcg gcttgcgcaa aacccgccgg gttcctggcg cgaacgcctg gaacgattgg
     7801 cgacgaccga ggcgccggaa ggcgacctgt tccgggcgca acagttgttg tcgcaatgga
     7861 aaagctatat tggccacctg ccggtgcacg acctgctgga tcggatctac tgcgaaggca
     7921 acgtggtggc gcgttacctc gccgcggcgg tgccgcactt gcgctcgcgc gtggaagcca
     7981 atctcaaccg tttcctcgaa ctggcgctgg agatcgacag cgggcgttac ccacgcctgt
     8041 cgcattttct cgcctatctc gaaagacaga ccgagaacga cgacaaggcg ccggcggaac
     8101 cggcctggaa tcgcgagccg cgcgtgcgtg tgctgaccat ccatgccg
//
LOCUS       DBNGZE010000006         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1875807, whole genome shotgun sequence.
ACCESSION   DBNGZE010000006 DBNGZE010000000
VERSION     DBNGZE010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1875807"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="AC16FV_00165"
     CDS             <1..1953
                     /locus_tag="AC16FV_00165"
                     /inference="COORDINATES: protein motif:HMM:NF051510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lnb N-terminal periplasmic domain-containing
                     protein"
                     /protein_id="HIR2848508.1"
                     /translation="CRFYISRDYHSMPGRWILLLILFWSPLAVFAADNAYLEELVQQA
                     RQARLSQRAEWLNLLHYKPYPYWPGSRSLADDPEFFNAPDGKTDASAELEATLAAFFS
                     TTEETDKTQNPQCRFIARYQWLKQELKFDSTRLPPQPCKRFYQWRASLDPHEITLVFP
                     AAYLNNPASMYGHTLLRIDARDQDERTRLLAYSISYAAGTDETSGLIFAVRGLFGGYP
                     GVFSITPYYLKVREYSDIENRDVWEYQLTLTPEEIDRLLMHVWELGPTHFDYYFFDEN
                     CAYHLLSLLEVARPGLQLTDRFRWWAIPSDTVRAVTDQAGLLKRVVYRPASVTIMQQR
                     LASMSADQRLLARDLGKQQISAKDSRVRELPALDQAKLLELGYEYAAYETTTGRDKIP
                     NAVTHMRELLLARSRLDIPPQKPEVPVPSVRPDEGHKTSRIGIGAGSRDGRRFGEIHL
                     RPTYHDLLDQDAGYTRGAQIQFFDMRWRHYEDNDGLRLEEFKPLDITSLSPRNDFFNP
                     VSWKVNIGWVRKRFPGDAEPLVFRLNGGPGLAYSAPGLFGGNAVYYGFIEASLDVNKH
                     FEQDYALGAGPAVGLLADITGRWGVNLQARSLRYGLGDDHWSREFTLEQRYALTRQSA
                     LRLNLSRKQEFRDYWNSANLSLYLYF"
     gene            1953..2801
                     /locus_tag="AC16FV_00170"
     CDS             1953..2801
                     /locus_tag="AC16FV_00170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2848509.1"
                     /translation="MRRLLVIGLLLLSLPGCGNVFFYPYPAHVQTPKQLGLAYEDVYF
                     NASDGTRLHGWFLPAQGRALGTILFLHGNAENISTHIMSVRWLPARGFNVFLLDYRGY
                     GSSEGKPTFAGVQDDAESALKMLIARPDVDASRIVVFGQSLGGAIAVYRVARTAYREN
                     IRALVVESAFSSYRRITREKLADFWLTWPFQYPLSWTVSDEYSPSRAVADISPIPLLI
                     IHGDQDPIVPLHHGQRLFELAREPKQLWIVQGGNHIQAFQSQRYRDRFVAYLTEILSK
                     APADHK"
     gene            2862..>2977
                     /locus_tag="AC16FV_00175"
     CDS             2862..>2977
                     /locus_tag="AC16FV_00175"
                     /inference="COORDINATES: protein motif:HMM:NF025116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR2848510.1"
                     /translation="MLTVVGRDQPGIVSGLTEALYKAGANLGEASMARLGGN"
BASE COUNT          577 a          884 c          866 g          650 t
ORIGIN      
        1 tgccgttttt atatctcccg tgattaccac tccatgccag ggcgctggat tttattgctc
       61 attctgtttt ggtcaccgct cgcggtattc gcggccgaca atgcctatct cgaagaactg
      121 gttcaacagg cccggcaggc caggctgtcg cagcgcgccg agtggctcaa cctgctgcac
      181 tacaagcctt atccttattg gcccggcagc cgcagtcttg ccgacgatcc cgaatttttc
      241 aacgcgcctg acggcaagac cgacgcatcc gccgaactgg aagcgactct cgctgctttt
      301 ttttccacta cggaggagac cgacaagaca cagaatccac agtgccggtt tatcgcgcgt
      361 taccagtggc tgaagcagga attgaaattc gactcaacgc gcctgccgcc gcagccctgc
      421 aagcgttttt accagtggcg cgccagcctc gacccgcatg aaatcacgtt ggtctttccc
      481 gccgcttacc tgaacaaccc ggcctcgatg tatggccata cattgctgcg catcgatgcc
      541 agggatcagg atgaaagaac acgattgctt gcttattcca tcagctacgc cgccggcacg
      601 gatgaaacca gcggtctgat cttcgcggtg cgcggattgt tcggcggtta tcccggtgtt
      661 ttttccatca cgccgtatta cctcaaggtc agggaataca gtgacattga aaaccgcgac
      721 gtgtgggaat atcagctgac gctcacgccg gaggaaatcg atcgcctgtt gatgcacgtg
      781 tgggaactgg gcccgacgca cttcgattat tattttttcg acgagaactg cgcctaccat
      841 ctgttgtcgc tgctggaagt ggcccgcccc ggtttgcagc tgaccgatcg cttccgctgg
      901 tgggcgattc cctccgatac cgtgcgcgcc gtgaccgacc aggcgggcct gctcaagcgc
      961 gtggtttacc gtcccgccag cgtcacgatc atgcagcagc ggctggcatc catgtcggcg
     1021 gatcagcgtc tgctggccag ggatctgggc aagcagcaaa tcagcgcgaa ggacagtcgt
     1081 gtgcgtgaat tgccagcgtt ggatcaggcc aagctgctgg agcttggtta tgaatacgcc
     1141 gcctatgaaa caaccaccgg tcgcgacaag atacccaacg ccgtgaccca tatgcgcgag
     1201 ctgttgctcg cgcgcagccg gctcgatatc ccgccgcaaa agccggaagt gccggtgcct
     1261 tcggttcggc cggacgaggg gcacaagacc tcgcgcatcg gcatcggggc aggaagccgg
     1321 gacggccgcc gtttcgggga aattcatctg cgtccgacct atcacgatct gctggatcag
     1381 gacgccggtt acacccgcgg cgcgcagata cagttttttg acatgcgctg gcgccattat
     1441 gaggacaatg acggcctgcg tctggaggaa ttcaagccgc tcgatatcac ctcactctcg
     1501 ccacgcaacg attttttcaa tccggtgtca tggaaggtca atatcggctg ggtgcgcaag
     1561 cgttttcccg gcgatgcgga accattggta ttccggctca atggcggacc cggactggcc
     1621 tacagcgcac cgggactgtt cggtggaaac gccgtttact acggttttat cgaggcgtcg
     1681 ctggacgtca acaaacattt tgaacaggat tatgccttgg gggctggccc tgccgtcggc
     1741 ctgctggccg atatcacggg acgctggggg gtcaatctgc aggcacgctc gttgcgctac
     1801 gggctcggcg acgatcactg gtcgcgcgag ttcaccctgg aacagcgtta tgcgctgaca
     1861 cggcaaagcg cgctgcgtct gaatctgtcg cgcaaacagg aattccgcga ctactggaac
     1921 agcgcgaatc tctcgctgta tctttatttc tgatgcgacg tctactggtc atcggcttgc
     1981 tcttgctcag ccttcccggt tgcggcaatg tttttttcta tccctatccg gcgcatgtgc
     2041 agacgcccaa gcaactgggg ctggcgtatg aagacgtgta cttcaacgcg agcgacggca
     2101 cgcgcctgca cggctggttt ctgcccgcgc agggcagggc gctcgggacg attctttttt
     2161 tgcacggcaa tgccgaaaac atcagcaccc acatcatgtc ggtgcgctgg ttgccggccc
     2221 gtgggtttaa cgtgttcctg ctggattacc gtggttacgg ttcctccgaa gggaagccca
     2281 cattcgccgg tgtgcaggat gacgccgaga gcgcgctgaa gatgctgatc gcacggcccg
     2341 atgtggatgc cagccgcatc gtggttttcg ggcaaagcct tggcggcgcc attgccgtgt
     2401 atcgcgtggc gcgtaccgcc taccgcgaga atattcgcgc gctggtggta gaaagcgcgt
     2461 tttccagtta ccgtcgcatc acgcgcgaaa aactcgccga cttctggctg acctggccgt
     2521 tccagtatcc gctctcgtgg acggtatcgg atgaatacag tccgtcccgg gccgtggctg
     2581 acatcagccc catcccgctg ctgatcatcc acggggatca ggacccgatc gtaccgctgc
     2641 atcacgggca acgtttgttc gaactcgcgc gtgaaccgaa acagttatgg atcgtgcagg
     2701 gcggtaatca catccaggcg tttcagagcc agcgctaccg cgaccgtttt gtcgcttatc
     2761 tgacggaaat cctgtccaaa gcaccggcgg atcacaaata aacgtaaccg ctgcttgcac
     2821 cgattatctt tcaagcgagg cacgatatga aacaatggtt catgctgacg gtcgtcgggc
     2881 gggatcaacc cggtattgtc tcgggcctga ccgaggcctt gtataaagca ggcgccaatc
     2941 tgggcgaggc ttccatggcg cggcttggcg gcaattt
//
LOCUS       DBNGZE010000007         3991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_70174, whole genome shotgun sequence.
ACCESSION   DBNGZE010000007 DBNGZE010000000
VERSION     DBNGZE010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_70174"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="AC16FV_00180"
     CDS             complement(<1..456)
                     /locus_tag="AC16FV_00180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR2848511.1"
                     /translation="MTHAEWIASFLGQLAGKGVTVEELAPAELIVVRTDDWGRLAEEA
                     HASGCRWVAGWGEDIGEEILINACFEKGGAYLIARTAVKRATPIVPSHTPYFPGADRP
                     ERHTQDMLGVAFIDHPDARQWTRHEAWKDHDYPLRREFPAAGNPPAQTPT"
     gene            complement(453..1928)
                     /locus_tag="AC16FV_00185"
     CDS             complement(453..1928)
                     /locus_tag="AC16FV_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HIR2848512.1"
                     /translation="MMLTLLEITLLTPLVGSLLLVFTGHRPIGGWINVLTGGVTFVAS
                     LMMALDVFAGGPTLSGNRMFYIDAFNVYLVTLTAFVGLTTAIFSRPYMRHEHERGRLT
                     GGRMRLYHAMYQGFLFAMLLALSTNNLGILWVAMEAATLTTVLLVSLYRTPESIEAAW
                     KYFVLCGVGIAQALFGTVLIYFAAEQKLGAGRDDTLLWSVLHAQASSLDPTVLSLACV
                     FLLVGYGTKVGLVPLHNWLPDAHSEGPTPMSAVLSGLLLNVALYALVRLKMLVDASLQ
                     NQLAGYLMMGFGLLSFMVAGLFLHRQHDIKRMFSYSSIEHMGLMTYAFGLGGALATFG
                     ALLHMTVHSLTKSAIFVTVGHASHIAGSQRIERIRGLIRTQPAVGWGLLIGTVAIAGF
                     PPFGVFASEFLLLMATMKSWPWLAVPLLVGFGIAIAGLFRHLHPMVYGEPPDGQKPVQ
                     ANMLPVAIHLALVLWLGLSIPGFLARWFEQATVLISGSSPL"
     gene            complement(1925..2650)
                     /locus_tag="AC16FV_00190"
     CDS             complement(1925..2650)
                     /locus_tag="AC16FV_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HIR2848513.1"
                     /translation="MILTQLTLYQQAIIVFAALVLFTSFIMLAQARIMPLIFAFAWQG
                     LLLSVVTALVAYVSDHPHLYISAAMTVVLKVIVIPYLLYRLARRLNLHYDAEQIKHPS
                     LLLLAGAGLVIFSYYVALPIVHLSGLATRNTIAVSIATVLIGMLLMIARKKAVAQVIG
                     FMSIENGLFFSAVVSTYGMPLIVELGVAFDVLVAAILFGVFFFHMRESFDTLDVDRLT
                     RLSETHEPPPLDAAEDTPPGGQP"
     gene            complement(2714..3097)
                     /locus_tag="AC16FV_00195"
     CDS             complement(2714..3097)
                     /locus_tag="AC16FV_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR2848514.1"
                     /translation="MDTDTNRLNRVTEAVIGCAYRVSSSLGCGFFEKVYENALVHELH
                     KTGLQVQQQKRFNVTYDGIQVGEYIADLVVESEVIVETKAVKVLEKIHLAQCLNYLKA
                     TGLKLGLVLNFGTPRVSVKRVVNRL"
     gene            complement(3136..>3991)
                     /locus_tag="AC16FV_00200"
     CDS             complement(3136..>3991)
                     /locus_tag="AC16FV_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR2848515.1"
                     /translation="MQNRAAPSLFQPYRDLAKLLRKHLVLAENASWLFRATPYIVFGA
                     MLISAAAVPLVALHLPTGAIADAIVIVGFFALARFFTALAGLDIGTAFGGMGSSREMT
                     IAALAEPAMLMAVFTLSMTASSTNLFAAVEQVMQAGLVLRPSFLFALLALVMVAVAET
                     GRVPVDNPATHLELTMIHEAMILEYSGRHLALIEWANQIKLMIYAVLIANLFWPWGIA
                     REFTPEALAVGTVAITAKLAGLAVALVIWETVLAKMRIFRVPQFLGFAFLLSLLGMLT
                     HVVLETGG"
BASE COUNT          848 a         1244 c         1255 g          644 t
ORIGIN      
        1 ggtcggcgtc tgcgccgggg gattcccggc cgccgggaac tcgcgccgca gcgggtagtc
       61 gtggtccttc cacgcctcat gccgcgtcca ctgccgtgcg tccggatgat cgatgaaggc
      121 cacgcccagc atgtcctggg tatggcgctc gggacggtcg gcgcccggaa aatacggcgt
      181 gtgcgagggc acaatgggcg tggcgcgttt cacggcggtg cgcgcgatca ggtaggcacc
      241 gcccttttca aaacaagcgt tgatgagaat ttcttcgccg atatcttcgc cccatccggc
      301 cacccagcgg cagccgctgg catgggcctc ttctgcgaga cgtccccagt cgtcggtgcg
      361 gacaacgata agttctgccg gcgccagctc ctcgaccgtt acccccttgc cggccaactg
      421 gccgagaaaa gacgcgatcc attcggcgtg cgtcatagcg gcgagctccc ggagatgagc
      481 acggtcgcct gctcgaacca gcgcgccagg aaacccggaa tggaaagccc gagccacagc
      541 accaaagcga gatggatcgc caccggcagc atgttggcct gcacgggttt ctgcccgtcg
      601 ggcggctcgc catagaccat cggatgcagg tggcgaaaca agcccgcgat ggcgatgccg
      661 aagccgacga gcagcggcac cgcgagccac ggccaggatt tcatggtcgc catgagcaac
      721 agaaactcgc tcgcgaacac gccgaacggc gggaagcccg cgatcgccac cgtgccgatc
      781 agcaaacccc aacccacggc gggttgggtg cggatgagac cgcggatgcg ttcgatgcgc
      841 tggcttccgg cgatgtgcga cgcgtgcccc acggtgacga agatcgcgct cttggtgagc
      901 gagtgcacgg tcatgtgcag cagcgcgccg aaggtcgcga gcgccccgcc gagcccgaag
      961 gcataggtca tgagccccat gtgctcgatc gaggaataac tgaacatgcg cttgatgtcg
     1021 tgctggcgat gcaggaacag gccagcgacc atgaacgaca gcaaaccgaa acccatcatg
     1081 aggtaaccgg cgagctgatt ttgcagcgat gcatccacca acatcttgag gcgcaccagg
     1141 gcatagagcg ccacgttgag cagcaaccct gagagcaccg ccgacatcgg cgtcgggccc
     1201 tcggagtgcg cgtccggcag ccagttgtgc agcggcacca accccacctt ggtgccgtag
     1261 cccacgagca ggaacacgca ggcgagcgac agcaccgtgg gatcgaggct cgacgcctgg
     1321 gcgtgcagca cgctccacag cagcgtgtcg tcgcgcccgg cgccgagctt ctgttcggcg
     1381 gcgaagtaga tgagcaccgt gccgaacagg gcctgggcga tgcccacgcc gcacagcacg
     1441 aaatacttcc acgccgcctc gatggactcg ggcgtgcggt agagcgacac cagcagcacg
     1501 gtggtcagcg tcgcggcctc catggccacc cacaggatgc cgaggttgtt ggtcgagagc
     1561 gcgagcagca tcgcgaacag gaagccctga tacatcgcgt ggtacaggcg catgcgcccc
     1621 ccggtcaggc ggccccgttc gtgttcgtgg cgcatgtagg ggcgcgagaa gatcgcggtc
     1681 gtgaggccga cgaaggccgt gagcgtgacg agatagacgt tgaaggcgtc gatgtagaac
     1741 atgcggttgc ccgaaagcgt cggaccgccc gcgaacacgt cgagcgccat catcagcgag
     1801 gcgacgaacg tgacgccgcc cgtcagcacg ttgatccagc cgccgatcgg acggtggccg
     1861 gtgaagacga gcagcaggct gccgacgagc ggcgtgagca gcgtgatctc cagcagcgtc
     1921 agcatcatgg ctgacctccc ggcggcgtgt cctcggccgc atcgagcggc ggcggctcat
     1981 gcgtctcgga caggcgcgtt agccggtcca cgtcgagggt gtcgaacgac tcgcgcatgt
     2041 ggaagaagaa cacgccgaac aggatcgccg ccaccagcac gtcgaaggcc acgcccagct
     2101 ccacgatcag cggcatgccg taggtcgaaa ccaccgccga gaagaacagg ccgttctcga
     2161 tggacatgaa gccgatcacc tgcgccaccg ccttcttgcg cgcgatcatg agcagcatgc
     2221 cgatcagcac ggtggcgatg ctgaccgcga tggtgttgcg cgtggcgagc cccgagagat
     2281 gcacgatcgg cagcgcgacg taatagctga aaatgacgag cccggcgccg gccagcagca
     2341 gcaacgaggg atgcttgatc tgctcggcgt cgtaatgcag gttgagccgc cgtgccaggc
     2401 ggtacagcag ataaggaatg acgatcacct tgagcaccac ggtcatggcc gcggagatat
     2461 acaggtgcgg gtgatccgac acgtaggcga ccagggccgt caccaccgac agcagcagtc
     2521 cctgccaggc gaaggcgaag atgagcggca tgatgcgcgc ctgcgccagc atgatgaagg
     2581 aggtgaacag caccagcgcg gcgaacacga tgatcgcctg ctggtaaagc gtgagctgcg
     2641 ttaatatcat tttatctctg taacgcagat ggacgcagat tcataaagaa gatgaacgca
     2701 gatagtttca tggttataag cggttcacga cacgttttac cgaaacgcgg ggcgttccaa
     2761 aattcaatac taagccaagc ttcaagcccg ttgctttaag ataattgaga cattgggcca
     2821 aatgtatttt ttcaagaacc ttaaccgctt ttgtttccac gatcacctca ctctcgacga
     2881 caagatctgc aatatattct ccgacctgaa tcccatcata tgtgacgttg aaacgtttct
     2941 gttgttgtac ctgcaatccg gttttgtgca attcatgaac cagtgcattt tcatacactt
     3001 tctcaaagaa tccgcaacca aggctgctgg atacgcgata agcacatccg atgaccgctt
     3061 ctgttaccct attcaacctg tttgtatctg tgtccatctg cggtttcgtc agcgttcatc
     3121 tgcggttcca aattattatc cgcccgtctc cagcaccacg tgcgtcagca tcccgagcag
     3181 cgacaacagg aaggcgaagc cgaggaactg cggcacgcga aagatccgca tcttggccag
     3241 caccgtttcc catatcacca gcgccacggc cagtcccgcg agcttggcgg tgatcgccac
     3301 cgttcccaca gccagtgcct cgggcgtgaa ctcgcgcgcg atcccccagg gccagaagag
     3361 gttggcgatc agcaccgcgt agatcatgag cttgatctgg ttggcccatt cgatcagcgc
     3421 cagatgccgc cccgagtatt cgaggatcat cgcctcatgg atcatggtga gttccaggtg
     3481 cgtggccgga ttgtccaccg gaacgcgccc ggtctcggcg accgccacca tgacgagcgc
     3541 cagcagcgcg aacagaaagg acggccgcag caccaggccg gcctgcatta cctgctccac
     3601 ggcggcaaac aggttagtcg aggaggcggt catcgagagc gtgaacaccg ccatcagcat
     3661 cgccggctcg gccagcgccg cgatcgtcat ttcgcgcgag gaccccatgc cgccgaaggc
     3721 ggtgccgatg tcgagaccgg cgagcgcggt gaaaaagcgc gccagcgcga aaaagccgac
     3781 gatcacgatg gcgtcggcga tcgcgccggt cggcagatgc agggccacga gcggcacggc
     3841 cgcggccgag atcagcatcg cgccgaacac aatataagga gtagcgcgga acagccacga
     3901 ggcgttctcg gccagcacca gatgcttgcg cagcagcttg gcaagatcgc ggtacggctg
     3961 gaacagcgac ggcgccgcgc ggttctgcat g
//
LOCUS       DBNGZE010000008         3638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_347972, whole genome shotgun sequence.
ACCESSION   DBNGZE010000008 DBNGZE010000000
VERSION     DBNGZE010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_347972"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1490)
                     /locus_tag="AC16FV_00205"
     CDS             complement(207..1490)
                     /locus_tag="AC16FV_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR2848516.1"
                     /translation="MIIALMLIGMLVLALINVPIAVALAFVAMLAIFFVQGPDMLPNL
                     ALVMLSGASSFPLLAIPLFILSGAIMNASSISRRLIAFASSLVGFIRGGLSMVSIGTS
                     VFFAEISGSAVADVAALGTILIPAMKNRGYSKEVAAATMSSAASLAIIIPPSIPMILY
                     AVMAQTSVVKLFVAGIIPGLLGALCMAGAAYYHAIKFNFPSEQSFRLAEVWRTFKEAL
                     WAFILPIAILGSIFGGIVTATEGAGMAVVAAIVVGGLIYRDLDWRELRQACIDGGVQT
                     AVVMLLVASSALIGTFLTEAQLPQALGNAITGVTSNKYIVLLLLNIAFLILGMFLHSA
                     AAIILVIPIVMPMVNAVGIDPIHFGLVVTLNLAIGQQTPPVASVLMTACAIAKSDIWE
                     TTKVNVPYIMALLFVLFLCTYVPWISLVLVNLFYG"
     gene            complement(1487..2029)
                     /locus_tag="AC16FV_00210"
     CDS             complement(1487..2029)
                     /locus_tag="AC16FV_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR2848517.1"
                     /translation="MRTAFERLLETIVIILVATLTLLVIAGFFFRYIGYSLAWYDETA
                     SVGLVWLTYYGSALAALKGAHIGVPGFVNAMPPHVRVWVTLFAEACVFLFFAVLAWTG
                     VQVLVVLAGDGMVSLPWVPLQLTDSVIPIGAVLFIVAEALRFPKVMRDARDIGFTDTE
                     TLEAISHGAPDAHASERKKR"
     gene            complement(2112..3095)
                     /locus_tag="AC16FV_00215"
     CDS             complement(2112..3095)
                     /locus_tag="AC16FV_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR2848518.1"
                     /translation="MPLTKTTLLTFAVASMLSIGTAAAQTEIKFGHVGEPGSLFDLSA
                     QEFAKRANAKLGDKAKVTIYGSSQLGGDAELMKKLKLGTVDLALPSTVMSSQVPLFGL
                     FEMPYLVKDRNHMAKIRDQVVVPSMEPAAEKAGYHIIAVWENGFRQITNSKKPIVKPE
                     DLQGLKLRVPQGGWRVKMFQAYGANPSPLALSEVFVALQTGVMDAQENPLAQIYPSRF
                     YEVQKYLSMTGHVYTQAYVTAGVSWSKLPNDVQKALADTAKEMEPVVYKIAADLDNDL
                     LEKLKKAGMQVNEADKNAFIKASAKIYEEFSNEVPGGKELIDKALALGKTS"
     gene            complement(3221..>3638)
                     /locus_tag="AC16FV_00220"
     CDS             complement(3221..>3638)
                     /locus_tag="AC16FV_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2848519.1"
                     /translation="GILGLYLFGLNASVSAAVGFISLFGVSAMDGILLISYIRRRLDE
                     GFGKEDAIIGAAQARMRQIFMTGFSACIGLVPAAISTGIGSQVQQPLACVIVGGMLLS
                     PICSLLVIPTLARLAMPTVRRTAARSGRVAETGPAE"
BASE COUNT          857 a         1044 c         1048 g          689 t
ORIGIN      
        1 ttgtggtctg aaatatctgc cgcgagagat cgcctacgga aaaatttgcg ccatggtcga
       61 aggcgcgagg atcgtacgcg ccgaattttc gaaatgaacg gcaagggcgg accgcgcacg
      121 ttgcggcatg aaaatcgcga cgtggcactc gggaaaatgc aggcgatggc cgagggcgcg
      181 gcgatcgtgc gcgccgagat gggccgctag ccgtagaaca agttcaccag caccagcgag
      241 atccacggca cgtaggtgca caggaacaac acgaacaaca gcgccatgat atagggaaca
      301 ttgaccttgg tcgtttccca aatgtctgac ttggcgatcg cgcaagccgt catcagcacg
      361 ctagccaccg gcggcgtttg ttggccgatc gccagattga gcgtgactac cagcccgaaa
      421 tgaatagggt cgataccgac cgcatttacc atgggcataa cgatcgggat gaccaggata
      481 atcgccgcgg ccgagtgcag gaacatgccc aggataagga aggcaatgtt gagcaggagc
      541 agcacgatat atttgttgct cgtgacgccg gtgatggcat tgccgagcgc ctgaggaagc
      601 tgggcttcgg tcaaaaacgt gccgatgagc gcagagctgg cgaccagcag catcaccaca
      661 gcagtctgta cgccgccatc gatacaagcc tgccgcaatt cgcgccaatc caggtcgcga
      721 taaatgaggc cgccgaccac gatggctgca accaccgcca ttcccgcgcc ctcggttgcg
      781 gtgacgatac cgccgaaaat gctgccgagg atcgcaattg gaagaatgaa tgcccacagc
      841 gcctccttaa aggttctcca tacctcggct agccggaaac tttgctcgct gggaaaatta
      901 aatttgatcg cgtggtagta cgctgcgccg gccatgcaaa gtgcacccaa caggccaggg
      961 ataatgccgg caacgaaaag cttgacgact gaagtctgtg ccatcacagc gtagagaatc
     1021 atcggaatcg acggcgggat gatgatggca aggctggcgg cggacgacat cgtggctgcg
     1081 gcgacttcct tggaatagcc acggttcttc atcgcaggaa taaggatagt accaagtgcc
     1141 gctacgtcgg ccacggccga accggaaatt tcggcaaaaa acaccgacgt gccgatggaa
     1201 accatcgaca gaccgccacg gataaagccg acgagcgatg atgcaaaggc gatcaggcgc
     1261 cgcgagattg acgaagcgtt catgatcgcg ccggaaagga tgaacagtgg gattgccagc
     1321 aacgggaaac ttgatgcgcc gctgagcatc accagtgcga gattgggcag catgtccggc
     1381 ccttgcacaa aaaaaatggc cagcatggca acgaaggcaa gcgcgactgc aatcggaacg
     1441 ttgatgaggg caagcaccag catgccgatg agcatcaggg cgatgatcac cgcttttttc
     1501 tctcgctagc atgcgcatcc ggtgcgccat gtgagatggc ttcaagcgtc tcggtgtccg
     1561 tgaaaccaat gtcgcgcgcg tcgcgcatca ctttgggaaa gcgcagcgcc tcagccacga
     1621 tgaaaagcac agcgccaatg gggattacgg aatcggtcag ctgcagcggc acccacggca
     1681 ggctgaccat tccatcgccg gcgagaacga ccaacacctg cacgccagtc caggccagaa
     1741 ccgcaaagaa cagaaagaca caagcctccg cgaaaagtgt cacccaaacc cgcacgtgcg
     1801 gtggcattgc gttgacaaag cccggcacgc cgatatgtgc gccctttagg gccgcgagcg
     1861 ctgagccata ataggtgagc cacacgagcc cgaccgaggc ggtttcgtca taccaggcca
     1921 atgaatatcc gatgtagcgg aagaaaaatc cggcgatcac cagtagcgtg agcgtggcga
     1981 ctaatatgat gacgatggtt tcgagcaggc gctcaaaggc tgtccgcaag gttgcaacag
     2041 tcacatcaca ccccggactt ttctcataag ataaagggcc cgccgactag gccggcccat
     2101 gctaaaccac gtcaggacgt cttgccgagc gccagcgcct tgtcgatcaa ctccttgccg
     2161 ccaggaacct cgttggagaa ctcctcgtag attttggcac tggccttgat gaaagcattc
     2221 ttgtcggctt cgttcacctg cataccggcc ttcttcagct tttcaagtaa gtcgttgtcg
     2281 agatcggccg cgatcttata gacgacgggc tccatttctt tcgcggtatc ggcgagcgcc
     2341 ttctgcacgt cgttgggcag cttcgaccag ctcacgccag ccgtaacata ggcctgcgta
     2401 tagacgtggc cagtcatcga cagatatttt tgcacctcgt agaagcgcga tggataaatc
     2461 tgtgccagcg gattttcctg cgcatccatc acgccggtct ggagcgcgac aaaaacttcc
     2521 gacaacgcga gcgggctagg attggcgcca taggcctgga acattttcac gcgccagccg
     2581 ccttgcggca cgcggagctt gaggccctgc aggtcttcag gcttcacgat cggcttcttg
     2641 ctgttcgtta tctggcggaa accgttctcc cataccgcga taatatgata gccagctttc
     2701 tcggccgccg gctccatgct tgggaccacg acctgatcgc ggattttggc catgtgattg
     2761 cgatctttga ccaagtacgg catctcgaac agaccgaata gcggcacctg cgacgacatc
     2821 accgtagagg gcaacgccag atcgacggtc ccgagcttga gctttttcat cagctcagca
     2881 tcgccgccga gctggctcga gccgtagatc gtcaccttgg ccttgtcgcc aagtttggca
     2941 ttggcgcgct tggcgaattc ctgcgccgac aaatcgaata gcgagccggg ttcgccaacg
     3001 tgaccaaatt tgatttcagt ttgcgcggct gccgttccaa ttgacagcat ggacgccacg
     3061 gcgaatgtga gcagagtagt ctttgtgagc ggcatgattt tcctcctaaa aattttatat
     3121 tttccgctgg cgggcccttt ttcaggtttc ccgccgcggt tgttatggtt ttgattatca
     3181 atacaaccct gcgaaatttc cacaagatag ccgattggcg ctattcagca ggccctgttt
     3241 cggcgacgcg cccactccgt gctgccgtcc ttcggacggt tggcatggcc agccgcgcca
     3301 gggtcgggat tacgagcaga ctgcagatcg gcgacagcaa catgccaccg acgatcacgc
     3361 aggcgagcgg ttgctgcacc tgcgagccga tgccggtgga aatcgctgcg ggcaccaggc
     3421 caatgcaagc ggaaaatccg gtcatgaaaa tctgccgcat acgggcttgc gcggctccga
     3481 tgatcgcatc ttccttgcca aatccttcgt ccaggcgccg gcggatgtag gagatgagca
     3541 ggatgccgtc catagccgac acgccgaaca gggaaatgaa gccaacagcg gccgacacgc
     3601 tggcgttcag accaaacaag tagagcccga gaattcca
//
LOCUS       DBNGZE010000009         1554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1806763, whole genome shotgun sequence.
ACCESSION   DBNGZE010000009 DBNGZE010000000
VERSION     DBNGZE010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1806763"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC16FV_00225"
     CDS             complement(<1..457)
                     /locus_tag="AC16FV_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR2848520.1"
                     /translation="MSRLILFNKPYGVLTQFTDTQGRETLATYIKIPGVYPAGRLDRD
                     SEGLLLLTDDGKLQQRLSDPRYKQPKTYWVQVEGEPDEPALERLRRGVILDGKRTQPA
                     EVRRMAEPALWPRNPPIRFRQSIPTSWLEITLTEGRNRQVRRMTAAVGFP"
     gene            542..>1554
                     /gene="icd"
                     /locus_tag="AC16FV_00230"
     CDS             542..>1554
                     /gene="icd"
                     /locus_tag="AC16FV_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR2848521.1"
                     /translation="MAFTHIKVPSGGKISVNEDYSLNVPANPVIAFIEGDGIGRDITP
                     VMRKVVDAAVAKAYGGKRSIAWMEVYAGEKAVATYGNNTWLPAETFQAVKEYVVSIKG
                     PLTTPVGGGIRSLNVSLRQELDLYVCLRPVRYFRGVPSPVKQPEKTDMVIFRENSEDI
                     YAGIEWPSGSPEAQKVIAFLQREMGVKKIRFPETSAIGIKPVSREGTERLVRRAIQYA
                     IANDRPSVTLVHKGNIMKFTEGGFKSWGYELAQREYGAQLIEQGPWMRLNNPKSGKEI
                     VIKDVIADAFLQQILLRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDTVA
                     MFEA"
BASE COUNT          305 a          445 c          493 g          311 t
ORIGIN      
        1 tcggaaatcc cacggctgcg gtcatgcgcc gcacctggcg attgcggcct tcggtgagcg
       61 tgatttccag ccatgatgtc ggtatcgatt ggcgaaaccg tatcggtgga ttacgcggcc
      121 acagcgccgg ttccgccatg cgccggactt ctgccggttg tgtgcgcttg ccgtcgagaa
      181 tgacgccgcg ccgcagccgc tccagcgccg gctcatccgg ttcgccctcg acctgcaccc
      241 agtaagtttt gggctgtttg taacgcggat cggacaggcg ttgctgaagc ttgccgtcat
      301 cggtgagcag cagcagtcct tcgctgtccc ggtccagccg gccggcgggg tagacgccgg
      361 gaattttgat gtaggtggcc agcgtttcgc gtccctgagt gtcggtgaac tgcgtcagga
      421 cgccataggg tttgttgaaa agaatcagcc gggacatggc gcgagtgtaa tgcgttagcg
      481 tggtccgcct cgccggcggg ttctggtgat agaatgcgcg gctattttat cggagcagat
      541 catggctttc acacatatca aggtcccttc cgggggaaaa atctcggtca acgaggatta
      601 ttcgctcaat gtccccgcca atccggtgat tgccttcatc gagggcgacg gcatcgggcg
      661 cgatatcacc ccggtcatgc gcaaggttgt ggacgcggcc gtcgccaaag cctatggcgg
      721 caagcgttca atcgcctgga tggaagtgta cgcgggtgaa aaagcagtgg cgacctacgg
      781 caataatacc tggctgccgg cggaaacttt ccaggcggtc aaggaatatg tcgtctcgat
      841 caaaggaccg ttgacgacgc cggtgggcgg cggcatccgc tcgctcaacg tgtcgttgcg
      901 tcaggaactc gatctttatg tctgtctgcg tccggtgcgt tacttcaggg gcgtgccgag
      961 tcccgtgaag cagcccgaga aaaccgacat ggtgatcttc cgggaaaact cggaagacat
     1021 ctacgccggc atcgagtggc catccggttc gcccgaggcg cagaaggtga tcgctttcct
     1081 gcagcgcgaa atgggcgtga agaaaatacg ctttccggaa acctccgcca tcggcatcaa
     1141 gccggtctcg cgcgagggca cggaacggct ggtgcgtcgc gccatccagt acgccatcgc
     1201 caacgaccgc ccctctgtga ccctggtgca caagggcaac atcatgaaat tcaccgaagg
     1261 cggattcaag agctggggct atgaactggc gcagcgcgag tacggcgcgc aactcatcga
     1321 gcaaggaccg tggatgcgtc tgaacaatcc gaagtccggg aaagagatcg tgatcaagga
     1381 tgtgatcgcc gatgccttct tgcaacagat cctgttgcga ccggcggaat acagcgtgat
     1441 cgccacactc aatctcaacg gcgattacat ctctgatgcg ctcgcggcgc aggtcggcgg
     1501 cattggcatt gcgccgggcg cgaacctgtc tgacaccgtg gcgatgttcg aggc
//
LOCUS       DBNGZE010000010         3408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1320703, whole genome shotgun sequence.
ACCESSION   DBNGZE010000010 DBNGZE010000000
VERSION     DBNGZE010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1320703"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC16FV_00235"
     CDS             complement(<1..292)
                     /locus_tag="AC16FV_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848522.1"
                     /translation="MTSQKPEASQPSTPAPLSLPGALALSPFRRDRLLKEMQSRVPGL
                     TSVTALYWHFAALDSELDARGRALLERLLTYGPAADAAHANPSTVPLLVIPRL"
     gene            complement(432..1259)
                     /locus_tag="AC16FV_00240"
     CDS             complement(432..1259)
                     /locus_tag="AC16FV_00240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2848523.1"
                     /translation="MRLQNTGKRARADGAGLRFAILGSGSRGNAALIECGRTALLLDC
                     GFSARETSARLARHDRHHGKLSAILITHEHSDHCRGVASCARQFGVSVWMTAGTYSAL
                     KGQFDADTDIRLINPHEAFAIGDIEVQPFPVPHDAREPCQFTFSNGDTRFGFLTDIGS
                     ITAHVVEMLSDCDALVLECNHDARMLRDGPYPPSLKARIAGEHGHLENKVAAELLSQV
                     MSPRLRYVAAAHLSETNNTPALAQAALARAMGCEPEWIDVAEQEEGLAFHHVAEVWV"
     gene            complement(1265..2377)
                     /gene="bamC"
                     /locus_tag="AC16FV_00245"
     CDS             complement(1265..2377)
                     /gene="bamC"
                     /locus_tag="AC16FV_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR2848524.1"
                     /translation="MTIRFARLLLCLLAAVSVGACQTVAEKRKIDYKSTRTLPPLDIP
                     PDLAAPEGAKDAKEGAPGAATYSEFASGEAQKKAPVGSDVLPAYDGIRLERAGQIRWL
                     VVKLPAEQLWPRVREFILDRGLIIDQENPQTGVLETDWAENRANVGTGMQKILAKSLG
                     TLYSTGTRDKYRIRLERGKEPGTTEIYLSHRGMVETIVGNDTSGVGETRWQPRPSDPD
                     LEAEMLRLLMASLGVKEEKAKDMVASAAATPAPERAKLNRQENGVFLSLQEDFERAWR
                     RVGLVLDRTSFTVEDRDRSKGIYYVRYIDPDAPKKKGWFRRLFSKKVPEPTNDYQIVL
                     KGNETATRVDVLTREGAPEQSKTSERILTLLYDQLK"
     gene            complement(2408..3286)
                     /gene="dapA"
                     /locus_tag="AC16FV_00250"
     CDS             complement(2408..3286)
                     /gene="dapA"
                     /locus_tag="AC16FV_00250"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR2848525.1"
                     /translation="MFHGSMVALVTPMNEDGSLDLPALRRLVDFHVENGTDAIVAVGT
                     TGESPTLDMDEHCEVIRVTVEQARDRVPVIAGTGANSTREAIELTRCAEKSGADACLL
                     VTPYYNKPTQEGLYLHHKAIAEAVPVPQILYNVPGRTACDMLPETVERLAAIPNIVGI
                     KEATGNLDRARDILRRCGDRIDLYSGDDATALDCILLGAKGDISVTANVAPKLMHEMC
                     KLGLERKEKEARAINDRLMGLHKNLFIEANPIPVKWALNQMRLIKGGIRLPLTPLSAR
                     QHETVRQAMRTAGITF"
BASE COUNT          564 a         1071 c         1098 g          675 t
ORIGIN      
        1 ccaaacgcgg gatgaccagc agcggcaccg tggagggatt cgcgtgggcg gcgtcggccg
       61 cggggccgta ggtcagcagg cgctccagca gcgcgcgtcc gcgcgcgtcg agctcgctat
      121 cgagcgcggc gaaatgccag tagagcgcag tcacactagt gagtcctggc acgcgggact
      181 gcatttcctt cagcagccgg tcgcgccgga acggcgacaa ggccagcgcg ccgggcaggg
      241 acagcggtgc cggtgtggac ggctgcgagg cttcgggttt ttggctcgtc atatcaccca
      301 gcttttgcta agaaacctct aagtcggttc gtgctgagct tgtcgaagca cgaaccgatc
      361 atcactgtta atttcttgcg ccccgttcac ccttcgacaa gctcagggcg aacgggattt
      421 aatcaggact gctacaccca aacctcggcc acatggtgaa acgccagccc ttcttcctgc
      481 tcggcgacgt cgatccattc cggctcgcag cccatggcac gcgccagcgc cgcctgtgcc
      541 agcgccgggg tgttgttggt ctcggacaga tgcgccgccg ccacgtagcg caggcgcggt
      601 gacatgacct gcgacagcag ttccgccgcc accttgttct ccaaatggcc atgttcgccg
      661 gcgatgcgcg ccttgagcga tgggggatag ggcccgtcgc gcagcatgcg cgcgtcgtga
      721 ttgcattcca gcaccagtgc gtcgcaatcg ctcagcatct ccaccacatg cgcggtaatg
      781 gagccgatgt cggtgagaaa accaaagcgc gtatcgccgt tggaaaaggt gaactggcaa
      841 ggttcgcgtg catcgtgcgg caccgggaac ggctgcacct cgatatcgcc gatggcgaag
      901 gcctcgtgcg ggttgatcag gcggatgtcg gtgtccgcgt cgaactggcc tttgagggcc
      961 gaatatgtcc cggccgtcat ccacaccgac acgccgaact ggcgcgcgca actggccacg
     1021 ccgcggcaat gatcgctgtg ttcatgggta atcaggatcg cggagagctt gccgtgatga
     1081 cggtcgtgcc gcgccaggcg cgcgctggtt tcacgcgcgg aaaatccgca gtcgagcagg
     1141 agcgcggtgc ggccgcactc gatcagcgcg gcgttgccgc ggctgccgct gccgaggatc
     1201 gcaaagcgca atccggcccc gtcagcccgc gcccttttgc ccgtgttctg cagtcgcaac
     1261 ccgcctattt cagttgatcg taaagcagcg tcaggatgcg ctcgctggtc ttggactgct
     1321 ccggcgcacc ctccctggtc agcacgtcca cccgggtggc agtctcattt cccttcagga
     1381 cgatctgata gtcgttcgtc ggctctggca ccttcttgct gaacaggcgc cggaaccagc
     1441 cctttttctt cggcgcgtcc ggatcgatgt agcgcacgta atagatgccc ttcgagcggt
     1501 cgcggtcctc gacggtgaag ctggtccggt ccagcaccag gcccacgcgc cgccacgcgc
     1561 gctcgaaatc ctcctgaaga ctgaggaaca cgccattttc ctggcgattg agcttggcgc
     1621 gctcgggcgc gggtgtcgcc gcggccgaag caaccatgtc cttcgccttt tcctctttca
     1681 cgcccaggct cgccatcagc aggcgcaaca tttccgcttc caggtccggg tccgaggggc
     1741 gcggttgcca gcgcgtctca cccacgccgg aggtgtcgtt gccgacgatg gtttcgacca
     1801 tgccgcgatg gctgagataa atttccgtgg tgcccggctc cttgccgcgt tcgagacgga
     1861 tgcggtattt gtcgcgcgtg ccggtggaat acagcgtgcc gagcgatttc gcgaggattt
     1921 tctgcatgcc ggtgccgacg ttcgcgcgat tctcggccca atcggtttca agcacgccgg
     1981 tttgcgggtt ttcctgatcg atgatgaggc cgcggtcgag gatgaattcg cgcacccgcg
     2041 gccacagctg ctccgccggc agcttgacca ccagccagcg aatctggccg gcgcgttcga
     2101 gtctgatccc gtcgtaggcc ggcaggacgt cgctgccaac gggagccttc ttctgcgcct
     2161 cgccgctggc gaattccgag taggtggcgg cgccgggcgc gccttccttc gcgtctttgg
     2221 ctccctcggg cgcggccaga tcgggaggaa tatcgagcgg cggcaacgtg cgcgtggatt
     2281 tgtagtcgat cttgcgtttc tcggcgacgg tctggcaagc cccgacggat acggccgcca
     2341 gcaggcaaag caggagtctg gcgaatcgta tcgtcatgga tcctcggcta gtgaataaaa
     2401 tcatcggtca gaatgtaatg cccgcggtgc gcatggcctg acgcacggtt tcatgctgcc
     2461 tggccgacag cggcgtcaac ggcaggcgga tgcccccttt gatcagtcgc atctggttta
     2521 gtgcccactt cacgggtatt ggattggcct cgatgaaaag gttcttgtgc agccccatga
     2581 ggcggtcgtt gatggcgcgc gcctcttttt ccttgcgttc cagcccgagc ttgcacatct
     2641 cgtgcatcag ctttggcgcg acgttggccg tgaccgagat atcccccttg gccccgagca
     2701 ggatgcagtc gagcgcggtg gcgtcgtcgc cggaatagag gtcgatcctg tcgccgcagc
     2761 ggcgcaggat atcgcgcgcg cggtcgaggt tgccggtggc ttccttgatg cccactatat
     2821 taggtatggc ggccagacgt tcgacggttt ccgggagcat gtcgcaggcg gtgcgcccgg
     2881 gcacattata tagtatctgc ggcaccggca cggcttcggc gatggccttg tgatggagat
     2941 acaaaccttc ctgcgtcggc ttgttgtaat aaggcgtgac cagaaggcag gcatcggcgc
     3001 cggacttttc ggcgcaacgg gtgagctcga tggcctcgcg cgtggaattg gcgccggtcc
     3061 cggcgatcac cggcaccctg tcgcgggcct gttccaccgt cacgcggatc acctcgcagt
     3121 gttcgtccat gtcgagcgtc ggcgactcgc cggtggtgcc gaccgcgacg atggcgtcgg
     3181 tgccgttctc aacgtggaaa tcgaccaggc ggcgcagcgc gggcaagtcc agcgacccgt
     3241 cctcgttcat gggcgtgacc aaggccacca tactgccgtg aaacataagg cgcaaacctg
     3301 aaaaattgaa tcggggaaag gcgcatggta cttgcgccct ccgccgatga caagccgcta
     3361 tccgcgcgtt aagcgccctg ttttatggcc cgatcagtgc attgtcat
//
LOCUS       DBNGZE010000011         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_209338, whole genome shotgun sequence.
ACCESSION   DBNGZE010000011 DBNGZE010000000
VERSION     DBNGZE010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_209338"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="AC16FV_00255"
     CDS             complement(<1..175)
                     /locus_tag="AC16FV_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HIR2848526.1"
                     /translation="MNPKGYLALVLHAHLPFVRHPEHERFLEEDWFFEGMTETYLPLL
                     NVFEGLARDGVHFR"
     gene            complement(184..1086)
                     /locus_tag="AC16FV_00260"
     CDS             complement(184..1086)
                     /locus_tag="AC16FV_00260"
                     /inference="COORDINATES: protein motif:HMM:NF027583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HIR2848527.1"
                     /translation="MPTKNLKELRAQARSRGLQGYSKLSRGDLEKLLSRHAGQKAAVV
                     EKTGKSAPKKPAASAKSKSAATRTKKKHAVPTARATAPATPAPALTPKWEWATDVQRQ
                     AADEEQVESAKYAIVPPGAAVPRDETADLNEDIDSLPPVTEPTLCLLPQKPGVLHGYW
                     ITPPNSLAHSRLLKLRLGRIAGDTFEIIEEIALPHERGHWYFHVDEAADTGAVYLQLG
                     YYEPDGRFVTAIRRGVARIPSLHASDRTDRLWWVSEEQFRTMYRRAGGYVHGPRLGWA
                     ASISSPGGAPSSDRFTRPGGVSNR"
     gene            1342..1548
                     /locus_tag="AC16FV_00265"
     CDS             1342..1548
                     /locus_tag="AC16FV_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HIR2848528.1"
                     /translation="MKIQKIRDIARGQGLQPGKAEKVELIRAIQRQEGNFDCFATAYD
                     GVCDQSGCLWREDCFATARSRKDS"
     gene            1582..>1793
                     /locus_tag="AC16FV_00270"
     CDS             1582..>1793
                     /locus_tag="AC16FV_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR2848529.1"
                     /translation="MFLRHVSSGDIVEVMDLEALFDPFRNEIKGRFHAGEEMPDPAGF
                     RKSDMMFLSGERLPRCWVDPHYKERK"
BASE COUNT          344 a          498 c          582 g          369 t
ORIGIN      
        1 cgcgaaagtg cacgccgtcg cgcgccaggc cttcgaagac attgagcaga ggcaggtagg
       61 tttcggtcat gccctcgaag aaccagtctt cctcgaggaa gcgctcatgc tccggatggc
      121 gcacgaacgg caggtgcgcg tgcagaacga gcgcgaggta acctttggga ttcaccgcgg
      181 gatttatcgg ttgctgacgc cgcccggccg ggtgaaacgg tcagaggagg gtgcgccgcc
      241 ggggctgctg atcgaggccg cccagcccag gcgcggtcca tgtacgtagc cacccgcgcg
      301 ccggtacatg gtgcggaact gttcctcgct cacccaccac aggcggtcgg tgcggtcgga
      361 ggcatgcagg ctcgggatgc gcgcgacgcc gcggcggatg gcggtgacga aacgcccgtc
      421 cggttcgtag tatccgagct gcaaatacac cgcgccggta tcggcggctt cgtccacatg
      481 aaaataccag tggccgcgct catgcggcag ggcgatttcc tcgataattt cgaaggtgtc
      541 gccggcaatg cgcccgagcc ggagcttgag taaccgggaa tgcgcaagtg agttcggcgg
      601 cgtgatccaa tagccgtgca gcaccccggg tttctgcggc aacagacaaa gcgtcggctc
      661 ggtgaccggt ggcaggctgt cgatatcttc attgagatcc gccgtttcgt cgcgcggcac
      721 ggcggcgccg ggcggaacga tggcgtattt cgcgctttca acctgttctt catccgcggc
      781 ttggcgctgt acgtccgttg cccattccca cttcggcgtt aacgccggcg ccggcgtggc
      841 aggcgccgtg gcacgcgcgg tcgggacggc atgcttcttt ttggtgcgtg tcgcggcgga
      901 ttttgacttg gcgctggcgg cgggtttttt gggcgcggat tttccggttt tttccaccac
      961 cgctgctttc tgtccggcgt ggcgcgacag aagcttttcc agatcaccgc gcgacagttt
     1021 ggagtacccc tgcagaccgc gggatcgggc ctgggcgcgc agttccttga ggtttttggt
     1081 cggcatggct cgggctgttg gcggaaaata cggcaaaaaa ttcacgaccg tctaatatcc
     1141 gacatacttc cactccgcgc cggaattgta aaggcccgtc accacgaagt ggaattaaac
     1201 gcttggcaca tggtgttgtc ggaccaaata tgatttattg atgtccgtca atgtcgagtc
     1261 acgtccgccg cgccatgctt ttgtcacatg gcaggcgcat ctttggaact ttcgaaacat
     1321 ctcaacaagg aggcgaggtc aatgaaaatc cagaagatac gcgatattgc ccgtggtcag
     1381 gggctgcaac cgggcaaggc cgaaaaagtc gagttaatca gggcgatcca gcgccaagaa
     1441 ggcaatttcg actgtttcgc cacggcctac gatggcgtgt gcgatcaaag cggttgcctg
     1501 tggcgcgaag attgtttcgc cacggccagg agccggaaag attcctgaag ccggcggcat
     1561 tagccaggta aaggaggtgc catgtttcta aggcatgttt cgagcggtga tattgtggaa
     1621 gtgatggatc tcgaagccct ctttgatcct ttcaggaacg aaatcaaagg gaggtttcac
     1681 gcgggtgagg aaatgccgga cccggccggg tttcgcaagt ccgacatgat gtttctatcc
     1741 ggcgagcgcc tgccgcgctg ctgggtggac ccacattaca aggagcggaa aat
//
LOCUS       DBNGZE010000012         1717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_695685, whole genome shotgun sequence.
ACCESSION   DBNGZE010000012 DBNGZE010000000
VERSION     DBNGZE010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_695685"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="AC16FV_00275"
     CDS             complement(<1..128)
                     /locus_tag="AC16FV_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR2848530.1"
                     /translation="MKKPIYLDYSATTPVDPRVAEKMCSYLTLDGEFGNPASRSHP"
     gene            complement(161..304)
                     /locus_tag="AC16FV_00280"
     CDS             complement(161..304)
                     /locus_tag="AC16FV_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848531.1"
                     /translation="MPVYLDHNATSPPEPRVVDAMLLFLLNHHGNISNVHGMVCATGL
                     AEV"
     gene            complement(328..783)
                     /gene="iscR"
                     /locus_tag="AC16FV_00285"
     CDS             complement(328..783)
                     /gene="iscR"
                     /locus_tag="AC16FV_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HIR2848532.1"
                     /translation="MKLTTKGRYAVTAMLDLALRYDKGAVTLADIAKRQGISLSYLEQ
                     LFAKLRRSGLVDSVRGPGGGYTLAMEPNKISVAEIIVAINENIDATRCGGEKNCHGDE
                     TCLTHQLWEDLSTRIHEFLSGITLGDLVTKPHVQEVASRQEGRMQVAVR"
     gene            complement(843..1637)
                     /gene="cysE"
                     /locus_tag="AC16FV_00290"
     CDS             complement(843..1637)
                     /gene="cysE"
                     /locus_tag="AC16FV_00290"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR2848533.1"
                     /translation="MFKNLREYLNSIFQRDPAARSVLEVLTAYPGVHALMWHRLSHRL
                     WRWRLRWLARMFSQLGRWLTGIEIHPGAKIGKRFFIDHGMGVVIGETAEIGDDVTLYH
                     GVTLGGTTWRKEKRHPTLGNNVVVGAGAKILGPITVGDNARVGSNSVVVKDVPAGATV
                     VGIPGRVIGPKKSDAKYQQREAMAKKIGFDAYGQSPDMPDPVAHAIDLILDHMHLVDQ
                     KMKIISSALEKTGVKVNLDMPELDVSNLEDPSPAIYPQRDKPKENS"
BASE COUNT          371 a          492 c          450 g          404 t
ORIGIN      
        1 aagggatgcg aacgcgaggc cgggttgccg aattcgccat cgagcgtcag atagctgcac
       61 atcttttccg ccacgcgcgg atcgaccggc gtggtggcgg agtaatcgag ataaattggc
      121 ttcttcatga tcttttcgtc gatatcattc cgtcagtatt tcaaacttca gccaagccgg
      181 tagcgcagac catgccatgc acattcgaaa tattgccgtg atggttcaga agaaacagca
      241 gcatcgcatc caccacccgc ggctcgggcg gcgaggtcgc gttatggtcc agataaactg
      301 gcatcgatga tcccgaagcg ctcaggacta gcgtaccgcg acctgcatcc tgccttcctg
      361 acgcgaggcc acttcctgca catgcggctt ggtgaccaga tcgcccagcg tgatcccgct
      421 gaggaactca tgaatgcgag tgctcaggtc ttcccacagc tgatgcgtca gacaggtttc
      481 atcgccatgg cagtttttct cgccaccgca acgcgtcgca tcgatgttct cgttgattgc
      541 cacaatgatc tcggcgaccg agattttatt gggctccatg gcgagggtgt aaccgccacc
      601 gggaccgcgg acgctgtcca ccagtccgct gcgacgcagc ttggcgaaca attgctcaag
      661 gtaagatagc gagatgccct ggcgtttggc gatgtcggcc aaggtgacgg cgcctttgtc
      721 atagcgcagc gccagatcca gcatggctgt cacggcataa cgacctttgg tagtcagttt
      781 cataggatat attcccacac ggtttatata cccttgcaat tctacttact tgatagtttt
      841 agtcaactat tctctttggg cttatctctt tgcggatata tggcaggtga tgggtcttca
      901 aggttactga catcaagctc gggcatgtcc aagttgacct taactcccgt tttttccagt
      961 gcgctcgaaa taattttcat cttctgatca acaagatgca tgtgatccaa gatgagatcg
     1021 atggcatgtg ccacggggtc cggcatatcc gggctctggc cataggcatc gaagccgatt
     1081 ttcttcgcca tggcctcacg ctgctggtat ttggcgtcgc tctttttagg accaatgact
     1141 cgccccggaa tgcccacgac cgtggctccg gccgggacat ccttcactac cacggaattc
     1201 gaccctacgc gggcattatc gcccacggta atcgggccca gaatcttggc gcctgccccc
     1261 accacgacgt tattacccaa cgtcgggtgg cgtttttcct tgcgccaggt ggtcccgccg
     1321 agggtcacgc catgatacag ggtgacgtca tcaccaatct cggcggtctc accgatgaca
     1381 acacccatgc cgtgatcgat gaaaaagcgc ttgccgattt tggccccggg gtggatttca
     1441 atgcccgtca gccagcgccc cagctgggaa aacatccgtg ccaaccagcg caggcgccag
     1501 cgccacagac ggtgcgacag gcgatgccac ataagcgcat gcacacccgg ataggccgta
     1561 agcacttcaa gcaccgagcg cgccgccgga tcgcgctgga aaatgctgtt caaatattca
     1621 cgcagatttt taaacatggc aggtaatatc ctgatttaaa tattataagt attcttgact
     1681 gttttaatca atttttagag cggccctgct ggatggc
//
LOCUS       DBNGZE010000013         3386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_487492, whole genome shotgun sequence.
ACCESSION   DBNGZE010000013 DBNGZE010000000
VERSION     DBNGZE010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_487492"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /locus_tag="AC16FV_00295"
     CDS             complement(<1..1801)
                     /locus_tag="AC16FV_00295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF039586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2848534.1"
                     /translation="MESRALKSETETPRPRTEGRQRTVTRYVTGFTPLAVLSVLLIAA
                     FIMMNAAAQNSALFGRLYSVLLLVNILGVVLLIALILLNLFHFVEQYRARVVGTRLTL
                     RLLVMFVVLAVVPVSVVFYFSIQALNRGIDNWFDVRIERALDDALLLGRTALDAQRQD
                     LVKTAQEIAVELETLPVRQKIGAVRSELSMLNALREQYDITELTLFTQDRQILASSSE
                     ANLEASTLIPDRPSDAVMVQVRQGQTYSNLDAISKTGLRLRVVVPVQAREVGAPIRVL
                     QVLQALPPRYAKLGESVQSAFAEYEKLVYLRGPLKFGFTLTLSLIALLTLLIAVWAAI
                     FFARRLATPIRELAEATHAVAQGDYQKQIPVTSHDEIGILVESFNDMTHRIHRAQVQS
                     KRSQQEAEIQRAYLETVLGHLSSGVLTFNQRLLLRTHNPAAGQILGADLRAGERKPLA
                     WLAENYPELRPLIEAVEQAAAAGQDEWHSQIEVSVNNHRRTLMLRGTSLPSWREKRGG
                     YVVVFDDITTLIQAQREAAWGEVARRMAHEIKNPLTPIQLSAERIRHKFLRQVVEEER
                     DTLERATRTIIEQVDSLKTMVNDFSEYARSAQMQ"
     gene            complement(1783..2310)
                     /locus_tag="AC16FV_00300"
     CDS             complement(1783..2310)
                     /locus_tag="AC16FV_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR2848535.1"
                     /translation="MLVLACSGNALADFKVTEIQPKFVEQSLVLTGNLELGLNTKVEE
                     ALAKGIPLEVNIDVHLQRERRFLWDRKIASWTLHRRIQYHALSGQYLISTAEPEPESG
                     ESLLTLQEALKQLGSLNGVALKLDEPVAPDATHSVDVRVSLDIEALPTPLRPVAYTSF
                     TWHLNSGWSTWKVAP"
     gene            complement(2333..>3386)
                     /gene="rsmB"
                     /locus_tag="AC16FV_00305"
     CDS             complement(2333..>3386)
                     /gene="rsmB"
                     /locus_tag="AC16FV_00305"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR2848536.1"
                     /translation="RYQLQFMRGAKHAAVKETVEAAAALKKPWAKNLLNACLRTTLRE
                     EARVQAAIAASPEAAFSHPAWLLKEIKHHWPENWAAILVANNERPPLVLRVNRLHQSR
                     EQYLARLAQAGLATSAHPFSENAVVLESPVAVSELPGFAQGDVSVQDAAAQLAALLLD
                     VQAGERVLDACAAPGGKTGHLLEQAALAELVALDREPARVKLIEENLARLGLTTKTIT
                     GDATNPASWWDGQAFDRILADVPCSATGVIRRHPDIKLRRRPEDLPRLVATQEQILDG
                     LWPLLKPGGKLLYATCSVLPAENENQMTAFLRRHPEATEEVLPTNAGRARTIGRQILP
                     GEAGMDGFYYARLRKN"
BASE COUNT          559 a         1086 c         1123 g          618 t
ORIGIN      
        1 actgcatctg ggctgagcgc gcgtattcgg agaaatcatt gaccatggtc ttgagcgaat
       61 ccacctgctc gatgatcgtg cgcgtggcgc gctcgagcgt gtcgcgttcc tcctccacga
      121 cctggcgcag gaacttgtgg cggatgcgct cggcggaaag ctggatgggc gtgagcgggt
      181 tcttgatctc gtgtgccatg cgccgcgcca cttcgcccca ggcggcctcg cgctgcgcct
      241 ggatcagcgt ggtgatgtca tcgaacacca cgacgtaacc gccgcgtttc tcgcgccagg
      301 aaggcagcga ggtgccgcgc agcatcaggg tgcggcggtg attgttgacc gagacctcga
      361 tctgggagtg ccattcgtcc tgcccggccg ccgcagcctg ttccaccgcc tcgatcagcg
      421 gccgcagttc cgggtagttt tccgccagcc aggcgagcgg tttgcgctcg ccggcgcgca
      481 ggtcggcgcc gagaatctgg cccgccgccg gattgtgcgt gcgcagcaac aggcgctggt
      541 tgaaggtgag cacgccggag gaaaggtggc ccagcacggt ttcgaggtag gcgcgctgga
      601 tctcggcctc ctgctggctg cgcttggact gcacctgcgc gcgatggatg cggtgcgtca
      661 tgtcgttgaa ggattcgacc aggatgccga tctcgtcgtg gctggtgacg ggaatctgct
      721 tctggtaatc gccctgcgcc accgcgtgcg tggcctcggc cagctcgcgg atgggggtgg
      781 cgagccggcg cgcgaagaaa atcgccgccc acaccgcgat cagcagcgtc agcagcgcga
      841 tcagcgacag cgtcagcgtg aagccgaact tgagcggccc gcgcaggtac accagctttt
      901 cgtattcggc gaaggccgac tgcaccgact cgcccagctt ggcgtagcgc ggcggcagcg
      961 cctgcagcac ctgcaagacg cggatcggcg cgcccacctc gcgcgcctgc actggcacca
     1021 ccacgcgcag tcgcaggccg gtcttgctga tggcgtcgag gttggaatag gtctgcccct
     1081 ggcgcacctg gaccatgacg gcgtcgctcg gcctgtcggg aatcagggta ctggcctcga
     1141 gattggcctc gctgctggag gcgagaatct ggcggtcctg ggtgaacaac gtcagttcgg
     1201 tgatgtcgta ttgctcgcgc agggcgttca gcatcgacag ctccgaacgc accgccccga
     1261 tcttctgccg caccggcagg gtttccagtt ccaccgcgat ctcttgcgcg gttttcacca
     1321 ggtcctggcg ctgcgcgtcg agcgcggtgc gcccgagcag cagcgcgtca tcgagggcgc
     1381 gctcgatgcg cacgtcgaac cagttatcga tgccgcgatt gagcgcctgg atggagaagt
     1441 aaaacaccac cgataccggc accaccgcca gcaccacgaa catcaccagc aggcgcaggg
     1501 tcaggcgcgt gccgaccacg cgcgcgcggt actgttccac gaaatgaaac aggttgagca
     1561 ggatcagcgc gatcagcagc accacgccga ggatgttgac cagcagcagc accgaataca
     1621 ggcgcccgaa cagggccgag ttctgggccg ccgcgttcat catgatgaac gcggcgatca
     1681 gcagcaccga cagcaccgcc agcggcgtga agccggtgac gtaacgcgtg acggtacgtt
     1741 gccgtccttc ggtccgtgga cggggcgttt cggtttcgct tttcagggcg cgactttcca
     1801 cgtgctccat ccgctgttga gatgccaggt aaacgaggta taggccaccg gccgcagggg
     1861 cgtgggcagg gcctcgatgt cgaggctgac gcgcacgtcc acggaatgag tggcgtccgg
     1921 tgccacgggc tcatccagtt tcagcgccac gccgttgagc gagccgagct gcttcagcgc
     1981 ctcctgcaag gtcagcaggc tttcgcccga ctcgggttcg ggctccgcgg tgctgatgag
     2041 atactggccg gacagcgcgt gatattgaat gcggcggtgc aacgtccagg aggcgatctt
     2101 gcgatcccac agaaagcggc gctcgcgttg caggtgcacg tcgatgttga cctcgagcgg
     2161 aatgcccttg gccagcgcct cttccacctt ggtgttcagc cccagttcga gattaccggt
     2221 gagcaccagc gactgttcca cgaacttcgg ttgtatctcg gtgaccttga agtccgccag
     2281 cgcattgccg gagcaggcca agacgagcac ggccgtggcc cagcagcgcc agttaattct
     2341 tgcggagtct cgcataataa aatccgtcca tgccggcctc gcccgggaga atctgcctgc
     2401 cgatggtccg cgcccgtccc gcgttcgttg gcaggacctc ctccgttgcc tcgggatgac
     2461 gccgcagaaa ggccgtcatc tgattttcgt tttcagccgg aaggaccgag caggttgcgt
     2521 acagaagctt acctccgggt ttgagcaagg gccacaggcc gtcgagaatc tgctcctgcg
     2581 tggcgaccag ccgcggcagg tcctcgggcc ggcgacgcag tttgatatcg ggatggcggc
     2641 gaatcacgcc ggtggcggaa cagggcacgt cggcgagtat ccggtcgaat gcctgtccgt
     2701 cccaccacga tgccggattc gtggcgtctc cagtaatggt tttcgtcgtc aggcccaacc
     2761 gcgcgagatt ttcctcgatc agtttcaccc gcgccggttc ccggtcgagc gccaccagct
     2821 ccgccagcgc ggcctgttcc agcaggtgcc cggtcttgcc gccgggcgcg gcacaggcat
     2881 cgagcacgcg ttcaccggcc tggacatcga gcagcagcgc ggccaactgc gccgcggcat
     2941 cctgcaccga gacatcgcct tgcgcgaatc cgggtagttc gctcacggcc acgggcgatt
     3001 ccagcaccac ggcgttctcg ctgaaaggat gggcgctggt ggcgaggccg gcctgtgcca
     3061 gacgcgccag atattgttcc cgcgattggt gcaagcggtt cacgcgcagc accagcggcg
     3121 ggcgctcgtt gttggccacc agaatcgcgg cccagttctc gggccagtga tgttttattt
     3181 ccttgagcag ccaggccgga tgactgaagg cggcttccgg gctcgcggcg atcgctgcct
     3241 gcacgcgcgc ttcctcgcgc agcgtggtgc gcagacaggc gttgagcaga tttttggccc
     3301 aaggtttttt gagagcggcc gcggcctcca cggtttcctt caccgcggcg tgtttggccc
     3361 cgcgcatgaa ctgtaattga taccgc
//
LOCUS       DBNGZE010000014         4860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_973878, whole genome shotgun sequence.
ACCESSION   DBNGZE010000014 DBNGZE010000000
VERSION     DBNGZE010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_973878"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16FV_00310"
     CDS             complement(<1..417)
                     /locus_tag="AC16FV_00310"
                     /inference="COORDINATES: protein motif:HMM:NF048427.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7666 domain-containing protein"
                     /protein_id="HIR2848537.1"
                     /translation="MTIKAWHFLREDKRLQFPPHTKVRTGQVLKVTGNPVLCSHGLHA
                     SIRPIDALGFAPGPIICRVEVGGIVVRGSDKLAGTERKVLWMADATETLRAFSRWCAL
                     EVIHLWNAPAVVVEYLKTGDESKREAAREAAWAATRE"
     gene            complement(457..717)
                     /locus_tag="AC16FV_00315"
     CDS             complement(457..717)
                     /locus_tag="AC16FV_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848538.1"
                     /translation="MSNATPSRESFFALYWDHVRSLPRLALMVMAKIPKDRMLDFESR
                     SWDELLECEREPIREAILFLIECHDATRQEAVRRRELQGKVA"
     gene            complement(808..1038)
                     /locus_tag="AC16FV_00320"
     CDS             complement(808..1038)
                     /locus_tag="AC16FV_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848539.1"
                     /translation="MSCACFFFVLGLGMHASNFDNDGRSLLSLDNPLGRIEAGYEFGN
                     GIEVKIEHTSQVQRIDTGLNLLLVQKRWEWKP"
     gene            complement(1035..1259)
                     /locus_tag="AC16FV_00325"
     CDS             complement(1035..1259)
                     /locus_tag="AC16FV_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848540.1"
                     /translation="MSAHRRHLITRKSDGAFWCGMFGFQSEPRDALTFDSLRSAYIYL
                     REICSDDSLTVEPADWYLPDHVLKPLECSP"
     gene            complement(1256..1477)
                     /locus_tag="AC16FV_00330"
     CDS             complement(1256..1477)
                     /locus_tag="AC16FV_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848541.1"
                     /translation="MTDNRKKDISWTLKTNSDGSTPSSNAHLAVLMDIRDEMKRLNDA
                     IYCVNFQRIPKTLKAIQRNTAKKKKKVRS"
     gene            complement(1486..1719)
                     /locus_tag="AC16FV_00335"
     CDS             complement(1486..1719)
                     /locus_tag="AC16FV_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848542.1"
                     /translation="MNAILHLNGVRVQGSTDKNFLMCSTPEGSFGISRERVAALRELL
                     GVMLTVTDPRTFEELIDGPDNIVPIMARPQAGG"
     gene            complement(1716..1958)
                     /locus_tag="AC16FV_00340"
     CDS             complement(1716..1958)
                     /locus_tag="AC16FV_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848543.1"
                     /translation="MDETAQAVIQLMQTVMAEIAKDNFHRERIKAAAHITAAILQNPK
                     VDPFENGVVYRAALYAGGAVNAIEQGIEVKGRGGAS"
     gene            2169..2660
                     /locus_tag="AC16FV_00345"
     CDS             2169..2660
                     /locus_tag="AC16FV_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848544.1"
                     /translation="MKPHTTIQFLDALKARHDLPSDYAARKILDVSKATISNYRVGKT
                     FFDDDVALKVAALLELDPGYVLACIHEERTKRPQVKEAWHKVAQALSPLFAAGIIGGT
                     ALLTPPPSPASELAGNIHYTHRRRGRFLPPRKPQDRRSLFSRPISHRQRPLPISGVLT
                     FCQ"
     gene            2673..3074
                     /locus_tag="AC16FV_00350"
     CDS             2673..3074
                     /locus_tag="AC16FV_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848545.1"
                     /translation="MLNTAEMADREILRFLPGRHQAVGVSRRTWRRWMAGETRIPRAV
                     VHLARILHGELGEISPAWSGWSLVGGQLVDPENVVHTPGSVQAWHWTAQQLSALRGEE
                     NQRGKIQRIRTGRDGQVVTLELHRRLTQSTP"
     gene            complement(3059..3451)
                     /locus_tag="AC16FV_00355"
     CDS             complement(3059..3451)
                     /locus_tag="AC16FV_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848546.1"
                     /translation="MSAPDKIAKLGGGVKDLAIGIGIVAAVAGIVYVAVKGKKAVDDA
                     TDAVKKGIGYVFGIDSLNPNASIGTDLFNATHAEPSSFDEPQAIKSCQAILDRNGRVI
                     GPICQQLLRQGKLVKKIPKDFVQGYGVD"
     gene            complement(3448..3612)
                     /locus_tag="AC16FV_00360"
     CDS             complement(3448..3612)
                     /locus_tag="AC16FV_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848547.1"
                     /translation="MKHEMQFDAEKHPNSSAVLKLAQEKFGPVQLVNIDWSTATATVE
                     TAEKKTAEKK"
     gene            complement(3634..3837)
                     /locus_tag="AC16FV_00365"
     CDS             complement(3634..3837)
                     /locus_tag="AC16FV_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848548.1"
                     /translation="MMELIVSILTSILAGAVAGGIAWGGMRAEVKGMRNDVQRLYQGN
                     EKAHTRIDDLNTRTARLEGRRRG"
     gene            complement(3834..4103)
                     /locus_tag="AC16FV_00370"
     CDS             complement(3834..4103)
                     /locus_tag="AC16FV_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848549.1"
                     /translation="MPLIQKILSLLIGLITMRPAQEGGATGAAGTTNSIAFYGALAGF
                     GAWLIGPGRDLEITLRGWEIWAVLLVGAILGALFLHIKPPQGPGP"
     gene            complement(4118..4672)
                     /locus_tag="AC16FV_00375"
     CDS             complement(4118..4672)
                     /locus_tag="AC16FV_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR2848550.1"
                     /translation="MMLEIKETGRIRSRDILIDAMRAKKFWIIGGVLLGLLLAGGGAA
                     VIYARTVESRQNEEKYRAAIAAAEFKYSLPDGLLHRLIFQESHFRTDIISGKTVSGAG
                     AVGIAQIVPKWHPGVDPLDPFASIDYAASYLKQLRKQTGSWSEAIAAYNWGLGNLKRS
                     GLENAPAETREYVASITADVPGLA"
     gene            complement(4617..>4860)
                     /locus_tag="AC16FV_00380"
     CDS             complement(4617..>4860)
                     /locus_tag="AC16FV_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848551.1"
                     /translation="AGVLRTTQVSGYVDTSCAGTAKVITRAAAYEHTAVASNTELASD
                     QALAVLHLTGTNFYLQFSDDAGNQGDGSYSIEGYFD"
BASE COUNT          955 a         1462 c         1454 g          989 t
ORIGIN      
        1 ctcccgcgta gcggcccacg cagcctcccg cgcagcctcc cgcttcgatt catctccggt
       61 ttttaaatat tcaacgacaa ccgcaggcgc attccataga tggatgactt ccagcgcgca
      121 ccagcgcgag aaagcgcgca gcgtttcggt agcatccgcc atccagagga cttttctttc
      181 agtgccggca agtttgtcgc tgccgcgcac gacgatgccg ccgacttcca cgcggcaaat
      241 gatcggtcct ggcgcgaaac ccagcgcatc gatcggcctg atcgatgcgt gcagaccgtg
      301 cgagcacaac accggatttc cagtcacttt tagcacctgg ccggtgcgca cttttgtgtg
      361 cggtggaaac tggagcctct tgtcttcacg aagaaaatgc catgccttga tcgtcatacc
      421 tttacctccc atatctggca ttaagttaaa aaatcgttac gccacttttc cctgcaactc
      481 gcgccggcgg acggcctcct ggcgcgtggc gtcgtggcac tcgatgagaa aaagaattgc
      541 ctcgcggatc ggttcgcgtt cgcactccag gagctcatcc caggagcgcg actcaaaatc
      601 gagcatgcgg tctttcggga tcttcgccat gaccatcagc gccaggcgcg ggagggagcg
      661 gacgtgatcc cagtagagag cgaagaagga ttcgcgggag ggagtggcgt tactcatcgt
      721 cttgctcgtc ttgaaaatcg agccacttcg cgagcgcggc cagggccacg agcgccagga
      781 gaatgatgcc agtaccgacg agccagatca cggcttccat tcccaacgct tttgcacgag
      841 cagcagattc aggccggtat cgatgcgctg cacctgcgag gtgtgctcga tcttgacctc
      901 gatgccgttg ccgaactcgt aaccggcttc gatgcggccg agcgggttat ctaagctgag
      961 cagcgagcga ccatcgttgt cgaagttcga cgcgtgcata ccgagaccga gaacgaagaa
     1021 aaaacaagcg cagctcatgg tgaacactcc aagggtttga gaacgtgatc ggggagatac
     1081 caatcggcgg gctcgacggt gagtgaatcg tcggaacaga tctcgcgcag atagatatag
     1141 gcgctcctga gcgaatcgaa cgtgagcgca tcgcgcggct ccgattgaaa gccgaacatg
     1201 ccgcaccaga aggcgccgtc ggacttgcgg gtgatgaggt ggcggcggtg ggcgctcatg
     1261 agcgcacctt cttttttttc ttcgcggtgt ttcgctgaat tgcttttagt gtttttggta
     1321 tccgctgaaa atttacgcaa tagatcgcat cgttaaggcg cttcatttcg tcacgaatgt
     1381 ccatgagcac agcgagatgc gcgttgctcg atggcgtgct tccatctgaa tttgttttca
     1441 gcgtccatga aatatctttt tttctgttgt ccgtcataac acggcttacc cgcccgcctg
     1501 cggccgcgcc atgatcggca cgatgttgtc cgggccatcg atgagctcct cgaacgtgcg
     1561 cggatccgtg acggtcagca tcacgcccag gagctcgcgc aacgccgcca cccgctcgcg
     1621 cgagatcccg aaagagcctt ccggcgtgct gcacatgaga aagtttttgt cggtggagcc
     1681 ctgaacccgg accccgttga gatgaagaat cgcgttcatg acgcaccccc ccggcccttg
     1741 acctcgatgc cctgctcgat cgcgttgacc gcgccgccgg cgtagagcgc cgcgcggtag
     1801 acgacgccgt tctcgaaggg atcgacttta gggttttgca gaatcgcggc ggtgatgtgt
     1861 gcggcggctt tgatgcgctc gcggtggaag ttgtctttgg cgatctccgc catgaccgtc
     1921 tgcatcagct ggataactgc ctgggctgtt tcgtccataa cgctctcctt gctgaaaggg
     1981 gcgtgacggc tagctgcgaa aggagacccg gaaacggttg ggaggatcca ggaggaacgc
     2041 gaccaaccgc cacgcttggg agcgtatata gtccaatgac cttgagaggt caagaggcat
     2101 tgaaccgttg accgtccaat gttcaaggac tagagtctgg agcctgcact cctgggagga
     2161 tcctgctgat gaaaccccac accacgattc aatttctcga cgcgctgaag gcgcgccatg
     2221 accttccgtc agattatgcg gcgcgaaaaa ttctcgacgt cagcaaggcg acgatcagca
     2281 attaccgggt aggaaaaact ttttttgacg acgacgtggc actcaaggtc gctgccctac
     2341 tcgagctcga tcccggctac gtcctcgcgt gcatccacga ggagcggaca aaacgccccc
     2401 aggtgaaaga ggcctggcac aaggtcgccc aggcgctctc ccccctgttt gccgcgggaa
     2461 tcatcggggg aacggcgctg ctgaccccgc ccccgtctcc ggcgtcagaa ctggcaggga
     2521 atatacatta cacgcaccgg cggcgaggcc gtttcctgcc gcccaggaag ccccaggatc
     2581 gacgatctct tttctcccgg cccatctccc accgccagcg cccgttgccg atttccggcg
     2641 ttttgacttt ttgccagtaa ccggcacggt ccttgctcaa taccgccgag atggcggata
     2701 gggaaatcct cagattttta ccagggagac atcaggccgt cggcgtgagc cggcgcacct
     2761 ggcggcgttg gatggccggc gagacgcgga tcccgcgcgc ggtcgtgcac ctggcgcgga
     2821 tcctccatgg cgagctcggc gagatctcgc cggcctggtc gggctggagc ctggtgggcg
     2881 gccagctggt ggatccggag aacgtggtgc acacgccggg gagcgtacag gcctggcact
     2941 ggaccgcgca gcagttgtcc gcgttacgcg gggaagaaaa tcagcgagga aaaattcagc
     3001 ggatccgcac cggccgcgat ggtcaggtgg tgacgctgga actgcaccgg cgactcactc
     3061 agtcgacgcc gtagccctgg acaaaatcct tcggaatctt tttgacgagt ttgccctggc
     3121 gcagcagctg ctggcagatc gggccgatga cgcggccgtt gcgatcgaga atcgcctggc
     3181 aggatttaat cgcctgcggt tcatcgaagg acgacggctc ggcgtgcgtg gcgttgaaca
     3241 gatccgtgcc gatgctcgcg ttgggattca gcgagtcgat gccgaagaca taaccgatgc
     3301 ccttcttcac ggcgtcggtg gcgtcatcga cggccttctt gcccttcacc gcgacataga
     3361 cgatgccagc cacggcggcg acgatgccga tgccgatggc gaggtccttg accccgccac
     3421 cgagcttggc aattttatcc ggcgcgctca cttcttctcg gcggtcttct tctcggcggt
     3481 ctcgaccgtg gcggtcgcgg tcgaccagtc gatgttcacc agctgcaccg ggccgaattt
     3541 ttcctgcgcg agcttcagga ccgcgctgct gttgggatgc ttctccgcgt cgaactgcat
     3601 ttcgtgtttc atgactacct ccgttggttg agattaaccg cgccggcgcc cttcgaggcg
     3661 cgcggtgcgg gtgttgagat catcgatgcg cgtgtgcgct ttttcgttgc cctgataaag
     3721 tcgttgcacg tcgttgcgca tgcccttcac ctccgcgcgc atgccgcccc aagcgatgcc
     3781 gccggcgacg gcaccggcga ggatggaagt caaaatcgag acgatgagct ccatcacggc
     3841 cccggcccct gcggcggttt gatgtgcagg aacaatgcac ccagaatcgc gccgaccagg
     3901 agcacggccc agatttccca gccacgcagc gtgatctcta aatcacgtcc ggggccgatc
     3961 agccaggcgc cgaagccggc gagcgcgccg taaaaggcga tgctattcgt ggtaccggcg
     4021 gcaccggtcg cgccgccctc ttgcgccggg cgcatggtga ttaatccgat gaggagagag
     4081 agaattttct ggatcagggg catgatagat ctccgtgtta agccagaccg ggcacgtcgg
     4141 cggtaattga tgcgacatat tcacgtgtct cagccggtgc gttttcgaga ccgctgcgct
     4201 tcagattgcc gaggccccaa ttataggcgg cgatggcttc actccaggaa cccgtctgct
     4261 ttcgcaactg cttgagatag ctcgcggcgt agtcgatcga ggcgaacgga tccaggggat
     4321 ccacgcccgg atgccacttg ggcacgatct gcgcgatgcc gaccgcgccg gcaccagaaa
     4381 cagtcttgcc gctgatgatg tcagtgcgaa agtgcgactc ctggaagatc aggcgatgca
     4441 gcaatccgtc gggcagcgaa tatttaaact cggcggcggc gatggcggcg cggtattttt
     4501 cctcgttctg ccgtgactcg acggtgcggg cgtaaatcac ggccgcacct ccgccggcaa
     4561 gcaacaggcc gagcaacacg ccgccgatga tccagaattt ttttgcgcgc atggcatcaa
     4621 tcaaaatatc cctcgatcga atacgacccg tctccttgat ttccagcatc atcgctgaac
     4681 tgcaaataaa aattcgtgcc ggtcagatgc aaaacagcca gcgcctgatc gctcgccagt
     4741 tctgtattag aggccacggc agtatgctca taggcggcgg cgcgcgtgat aacttttgcg
     4801 gtaccggcac agctcgtatc cacatatcct gacacttgag tcgtcctcaa aactccggcg
//
LOCUS       DBNGZE010000015         6640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1529633, whole genome shotgun sequence.
ACCESSION   DBNGZE010000015 DBNGZE010000000
VERSION     DBNGZE010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1529633"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC16FV_00385"
     CDS             complement(<1..374)
                     /locus_tag="AC16FV_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR2848552.1"
                     /translation="MASGRQEQDAQQTGELILQIVRRLAAELQPTRTPAPAATLDSLL
                     DRELGFDSLGRVELLARIERALDISLPERVLVTAETPRDLLRAVQQAAGPAGPGPTLP
                     AVQPATRDEASETPATADTLLEV"
     gene            complement(387..1850)
                     /gene="glgA"
                     /locus_tag="AC16FV_00390"
     CDS             complement(387..1850)
                     /gene="glgA"
                     /locus_tag="AC16FV_00390"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR2848553.1"
                     /translation="MPKILFVASEAHPLVKTGGLGDVAGSLPVALQSLRADVRLLLPA
                     YRDAMARAGKLKPVTTLSIPGLNAPVRIMEGKLPETSLITWLVDFPPAYDRPGNPYLD
                     LHGQPWPDNAMRFALLAHAATALALGRTQLKWRPDVVHCHDWQTGLVPVLLASEKSRP
                     ATVFTIHILSYQGLFPPKTFKALGLAPALWSPEALEFHGRMSFIKGGLVFADQLTTVS
                     PTYAREIQTPEFGDGLDGLLRHRAARLTGILNGIDDAQWDPVRDRFLVKHYSSRQLRN
                     KAANKLALQQELGLPPETDTPLISMVGRLVQQKGIDLIIDTLPGLMHRPLQLAVLGSG
                     ETGYEETLRKQAVRHAGRLALRIGYDEGLAHHIEAGSDMFLMPSRFEPCGLNQLYSLR
                     YGTVPIVRRAGGLADTVVDATEENIKSGKATGIVFQDARAAALLGAVDRALALRRDAV
                     RWKQIMLAGMRQDFSWDRSAEEYLRLYRQALRIKHGS"
     gene            1985..3253
                     /gene="glgC"
                     /locus_tag="AC16FV_00395"
     CDS             1985..3253
                     /gene="glgC"
                     /locus_tag="AC16FV_00395"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR2848554.1"
                     /translation="MNPAQSSQHITRLTRDTLALILAGGRGSRLKHLTLWRAKPSVPF
                     GGKFRIIDFPLSNCMNSGIRRVGVLTQYKAHSLIKHIQRGWGFLRGELGEFVELLPAQ
                     QRIETSWYTGTADAIYQNLDIIRNHDPKYVLILAGDHVYKMDYGSMLLHHIDNRADVT
                     VACVEVPVARAREFGVMTMNEENRIVRFQEKPENPEPLPGRPDTALASMGIYLFNTKF
                     LYEQLIRDADTAYSSHDFGKDVIPSIIDSYRIMAYPFRDPVTGRQPYWRDVGTLDAFW
                     EANIELVGVTPELNLYDAKWPIWTYQEQLPPAKFVFDDDDRRGMAVDSMVSGGCVISG
                     SRIRHSLLFSDVKLRSYSEIVDAVVLPDVIINRHCRLKRVVIDKGCDIPEGTVIGENF
                     QEDAKRFYVSPGGVVLVTPEMLGQELHHAR"
     gene            3243..4949
                     /locus_tag="AC16FV_00400"
     CDS             3243..4949
                     /locus_tag="AC16FV_00400"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR2848555.1"
                     /translation="MPANIRLKVVLCWHMHQPQYRDLVSGTYHLPWTYLHAIKDYTDM
                     AAHLEAVPAARAVVNFAPLLLEQIEDYARQVEGFLTNSLAIRDPVLAALISPALPPSN
                     EERIGLIKACARANEMRMIGRFPAYHRLAEMAAWFMAHPQSLRYLSNQYLADLLVWYH
                     LAWLGETVRRGDTRIKYLIEKESGYTLHDRRELLTVIGELLSSVIPRYKKLAARGQVE
                     LSVTPYAHPILPLLLDFSCAREAMPQAPLPLLPQYPGGEDQARWHVREGIETFRRHFG
                     FAPVGCWPSEGAVSTPSLKLLAEAGFRWAASGEGVLHNSLARSGHADHKMKEAWLYRH
                     YAVADSGMSCFFRDDGLSDLIGFTYATWHADDAVNNLVHHLENIAHCCRAHPEHVVSI
                     IMDGENAWEHYPENAFYFLSALYRRLSEHPGIELTTFSDCLKTVPAKDLRQLVTGSWV
                     YGTLSTWIGEPDKNRGWDMLGDAKRAFDAAAKRLGAEQLERARRQLAVCEGSDWFWWF
                     GGDNPAATVADFEQLYRQHLTNLYQLIGAEPPEYLAQVFTRGGGAPVHGGTMRTGQLP
                     VG"
     gene            4946..6421
                     /gene="malQ"
                     /locus_tag="AC16FV_00405"
     CDS             4946..6421
                     /gene="malQ"
                     /locus_tag="AC16FV_00405"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR2848556.1"
                     /translation="MIPAVLEQRRAGVLLHPTSLPGGIGNGDLGVDAHRFVDFLAQSG
                     FSVWQMLPLGPTHRDGSPYHSLSLHAGNPMLISLEQLAEWGWLENRDPAEAENPVTYR
                     LDRLAQAHRIFAQRASAAERDALAAFVATEAHWLHDYALYRALRREFSGQAWFQWPTP
                     LRDREPQALATARMRLTQDIVQVYFEQFVFARQWQALRAHAHERDLLLFGDMPIFVAH
                     DSVDVWAQRDYFMLDADGQPTVVAGVPPDYFSAQGQRWGNPLYHWKRMQTDGFRWWIA
                     RMTTEFRRFDLLRVDHFRGFEACWEIPAADETAVNGRWVKAVGEALFEVLRSHFGTLP
                     LVAEDLGLITPEVLALRDKFELPGMEILQFAFDSDADNPYLPHNHRKRSVVYTGTHDN
                     DTTLAWFENLPAERQLRVVEYLGYPHEPMPWPLVRAALASVSQLAIVPMQDILGLGRG
                     QRMNTPGTTEGNWCWRFAWEQLPPDLPERLRRMARMYGRTD"
BASE COUNT         1180 a         2006 c         2096 g         1358 t
ORIGIN      
        1 acctcgagta gcgtatcggc ggttgccggc gtctcactgg cctcgtcccg ggtcgcgggc
       61 tgcaccgccg gcagtgtcgg gcccggcccg gccggcccag cggcttgctg taccgcccgc
      121 agcagatcac gcggggtttc tgcggtcacc agcacgcgtt ccggcaaact tatgtcgagt
      181 gcacgttcaa tacgggcgag caattccacc cttcccaggc tgtcaaatcc cagttcacgg
      241 tccagcagac tgtcgagtgt tgccgctggg gcgggggttc tggttggctg cagttcggcg
      301 gcgagacgcc gtacgatctg aaggattagc tcaccagtct gttgcgcgtc ttgttcctgc
      361 cgtccgctgg ccatgatcgt ctctttttat gatccatgtt tgatccgcaa ggcttgcctg
      421 tagagccgca ggtactcctc tgcgctgcgg tcccaggaga agtcttggcg catgccggcg
      481 agcatgattt gtttccagcg aacagcatct cgccgcagcg ccagcgcgcg gtccacggcc
      541 ccgagcaggg cagcggcgcg ggcatcctga aacacgatgc cggtcgcctt gccgcttttg
      601 atgttttctt ccgtggcatc caccacggtg tcggccagcc cgccggcacg gcgaacgatg
      661 ggaacggttc cgtaacgcag gctgtagagc tggttcaagc cgcagggctc gaaacgcgac
      721 ggcatcagga acatgtccga tccggcctcg atgtgatgcg ccagcccctc gtcatagccg
      781 atgcgcagcg ccagacgtcc ggcatgacgc accgcctgct tgcgcagcgt ttcttcgtat
      841 ccggtttcac cgctgcccag caccgcgagt tgcagcggtc ggtgcatgag gccgggcagc
      901 gtgtcgataa taaggtcgat acctttctgc tgcacgagtc ttccgaccat gctgatcaac
      961 ggcgtgtcgg tttctggtgg cagacccaac tcctgttgca atgccagctt gttggcggct
     1021 ttgttgcgca gttgacgtga ggagtaatgc ttgacgagaa atcggtcgcg taccgggtcc
     1081 cattgcgcat cgtcgatgcc gttcaggatg ccggtcagcc gcgcggcgcg atgacgcaac
     1141 aggccgtcca gcccgtcacc gaattccggc gtctggattt cccgcgcgta ggtggggctg
     1201 acggtggtca actggtcggc gaatacgagc ccgcccttga tgaaggacat ccgtccatga
     1261 aactcgagcg cttccggcga ccagagcgcg ggcgcaagac cgagagcctt gaaggtcttg
     1321 ggcgggaaaa gaccctggta cgacaggatg tgaatggtga agaccgtggc cggacgtgat
     1381 ttttccgacg ccagcagcac cggtaccaag cccgtctgcc agtcgtggca atgcaccacg
     1441 tcaggacgcc acttgagctg ggtgcggccg agggccaacg ccgttgccgc atgcgccagc
     1501 agcgcaaagc gcatggcatt gtccggccat ggttgaccgt ggagatcaag atagggattg
     1561 ccgggtcggt cgtaagccgg cgggaaatcg accagccagg tgatgagcga cgtttcggga
     1621 agtttgcctt ccatgattct caccggcgcg ttgagaccgg gaatacttaa tgttgttacc
     1681 ggtttgagct taccggcacg tgccatggcg tcgcggtagg cgggcagcag caggcgcacg
     1741 tcggcgcgca gggattgcag cgcgaccggc aggctgccgg ccacgtcacc caggccgccg
     1801 gtcttgacca gcggatgggc ctcgcttgcg acgaacagga ttttcggcac gtttatttcc
     1861 ggacttggaa aaccgattcg ggatggctag tgtatttgaa ttcatggggt tgtggtaaat
     1921 tcagaattca tcgccgtata gggataagta cgcgggacag ccgataccag caggtggtgc
     1981 gaccatgaat ccggcccaat cttcgcaaca catcaccagg ctgacacgcg ataccctggc
     2041 gctgatcctc gccggcggac gcggatcgcg actgaaacac ctcacgctgt ggcgcgccaa
     2101 accctcggtg ccattcggcg gcaagttccg cattattgat tttccgcttt cgaactgcat
     2161 gaactccggc atccggcgcg tcggcgtgtt gacgcagtac aaggcgcatt cgctcatcaa
     2221 gcacatccag cgcggctggg gattcctccg cggcgaactc ggcgaattcg tggagctgtt
     2281 gccggcccag caacgcatcg aaacctcgtg gtacaccggt accgcggacg cgatttacca
     2341 gaatctcgac atcattcgca atcacgatcc gaaatacgtc ctgatcctcg cgggcgatca
     2401 cgtctacaag atggactatg gttcgatgct tttacatcac atcgataaca gggcggatgt
     2461 cacggttgcc tgcgtcgagg tgccggtggc gcgggcgcgc gaattcggcg tgatgaccat
     2521 gaacgaggag aatcgcatcg ttcgttttca ggagaagccg gaaaatcccg aaccgcttcc
     2581 gggtcgcccc gataccgcct tggcctccat gggaatttat ctgttcaaca ccaaattcct
     2641 gtacgagcaa cttattcgcg acgccgacac ggcctattca tcgcacgact tcggcaagga
     2701 cgtcattccc tcgatcatcg actcctatcg catcatggcc tatccgttcc gcgatccggt
     2761 tacgggacgg cagccgtact ggcgcgatgt tgggacgctg gacgcctttt gggaggcaaa
     2821 tatcgagctg gtcggtgtca cccccgaact caatctctac gatgccaagt ggccgatctg
     2881 gacctatcag gaacaactcc cgccggcgaa gttcgtgttc gacgacgacg atcgtcgcgg
     2941 catggcggtg gattcgatgg tgtcgggcgg ctgcgtcatc tccggttcgc gcatccgcca
     3001 ttcgctgctg ttttccgacg tgaagctgcg ctcctacagc gagatcgtgg acgccgtggt
     3061 gctgcccgac gtcatcatca atcgtcattg ccggctgaag cgggtggtga tcgacaaagg
     3121 ttgcgacata ccggagggta cggtgatcgg cgagaatttt caggaggatg ccaaacgctt
     3181 ctatgtgagt cccgggggcg tggtgctggt aacgccggag atgctgggcc aggaattgca
     3241 tcatgcccgc taacatacgg ttaaaagtgg tgctgtgctg gcatatgcac cagccgcaat
     3301 accgcgatct cgtaagcggc acttatcact tgccttggac ctatctgcac gccatcaagg
     3361 actacaccga catggcggcg cacctggagg ccgtgcccgc tgcgcgcgcc gtggtcaatt
     3421 tcgccccgct cctgctcgaa cagatcgagg attacgccag gcaggtcgaa ggctttctca
     3481 ccaacagcct cgccatacgc gatccggtgc tggcggctct aatcagtccc gcgctgccac
     3541 catcgaacga ggaacgcatt ggactcatca aggcctgtgc gcgcgccaat gagatgcgta
     3601 tgatcggccg ttttcccgcc tatcatcgtc tggccgagat ggccgcctgg ttcatggcgc
     3661 atccccaatc gctgcgctac ctgagcaacc agtatctcgc cgatctgctg gtgtggtatc
     3721 acctggcctg gctcggtgag accgtgcgcc gcggcgacac gcgcatcaaa tatctgatcg
     3781 agaaggaatc cggctacact ttgcacgacc ggcgcgaatt gctcaccgtc attggcgaat
     3841 tgttgtcgag cgtgataccg cgctacaaga aactggccgc gcgcgggcag gtggagcttt
     3901 ccgtgacgcc gtatgcccat ccgatcctgc cgttgctgct cgatttttcc tgcgcgcgcg
     3961 aggccatgcc gcaggcgccg ctaccgctgt tgccgcagta tcccggcgga gaggatcagg
     4021 cgcgctggca tgtccgcgaa ggcatcgaga cattccgccg gcatttcggt ttcgcgccag
     4081 tgggttgctg gccgtcggaa ggcgcagtaa gcacaccgtc cctgaaactg ctggcggaag
     4141 ccggcttccg ctgggcggcg agcggcgagg gtgttttgca caacagcttg gcccgatcgg
     4201 gtcacgcgga tcataagatg aaagaggcct ggctttaccg gcactatgcc gtcgcggaca
     4261 gcggcatgag ctgtttcttc cgcgatgacg gtttgtcgga tctcatcggt ttcacctacg
     4321 ccacctggca cgccgacgac gccgtgaaca acctcgtgca ccatctggaa aacatcgcgc
     4381 actgttgccg cgcgcatccc gagcacgtgg tatcgatcat catggacggt gagaacgcct
     4441 gggagcacta cccggagaac gccttttatt tcctgagcgc gctataccgc cggctgtcgg
     4501 agcatcccgg catcgaactg accacttttt ccgattgcct gaaaaccgtg cccgcgaaag
     4561 atttgcgcca gctcgtgacc ggatcgtggg tgtacggcac gctgtcgacc tggatcggcg
     4621 agccggacaa gaaccgcggc tgggatatgc tcggcgatgc caagcgcgcc tttgacgccg
     4681 ccgccaaacg gcttggcgcg gagcagctcg agcgcgcgcg gcgccagctc gccgtgtgcg
     4741 aaggctcgga ctggttctgg tggttcggcg gtgacaatcc ggccgcgacg gtggccgact
     4801 tcgagcagct ttaccgccag cacctgacca atctttatca gctgatcggt gcggaaccgc
     4861 cggaatatct ggcgcaggtc ttcacccgcg gcggcggtgc tcccgtgcac ggcggcacga
     4921 tgcgcaccgg ccagcttccc gtcggatgat tcccgccgtc ctcgaacagc ggcgtgccgg
     4981 ggtgctgttg catcccacct cgctgccggg cggtatcggc aacggtgacc tcggcgtgga
     5041 cgcgcaccgc ttcgtggatt tcctggcaca gtccggcttc agcgtctggc agatgctgcc
     5101 gcttggcccc acgcatcgcg acggctcgcc ataccattcg ttgtcattgc acgccggcaa
     5161 ccccatgctt atcagtctgg agcagctggc ggaatggggc tggctcgaga atcgcgatcc
     5221 tgccgaggcg gagaatccgg tgacgtatcg cctcgatcgt ctcgcgcagg cgcaccggat
     5281 tttcgcgcaa cgcgcttccg cggccgagcg cgatgcgctc gcggcgtttg tcgccaccga
     5341 ggcccactgg ctgcacgact atgcgcttta ccgggcactg cgtcgggaat tttccggtca
     5401 ggcctggttc cagtggccga cgccgctgcg cgatcgtgag ccgcaagcgc tggcgacggc
     5461 gcgcatgcgc ctgacccagg atatcgtcca ggtgtatttc gagcagttcg tgtttgcgcg
     5521 ccagtggcag gcgctgcggg cgcatgccca cgagcgcgat ttgctgctgt tcggggatat
     5581 gcctattttt gtggcacatg acagcgtgga tgtctgggcc cagcgcgact acttcatgct
     5641 cgatgccgac ggccagccga cggtggtggc gggcgtgccg ccggattact tttcagcaca
     5701 gggtcaacgc tggggcaatc cgctgtatca ctggaagcgc atgcagacgg atggttttcg
     5761 ctggtggatt gcgcgcatga ccactgaatt caggcgcttc gatttgctgc gcgtggatca
     5821 ttttcgcgga ttcgaggcct gctgggaaat accggctgcg gatgaaaccg cggtgaatgg
     5881 acgctgggtg aaggcggtgg gcgaggcgtt gtttgaagtc ttgcggtcgc atttcggcac
     5941 gctcccgctg gtggcggagg atcttggcct gatcacgccc gaagtgctgg cgttgcgcga
     6001 caagtttgaa ctgcccggca tggagattct gcaattcgcg tttgacagcg acgcggacaa
     6061 tccctatctg ccgcacaacc atcgcaagag gagcgtggtt tacaccggca cgcacgacaa
     6121 cgacaccacg ctcgcgtggt tcgagaatct gccggcggaa cggcaattgc gcgtggtgga
     6181 atatctcggc tatccgcacg aaccgatgcc gtggccgctg gtgcgtgccg cgctcgcctc
     6241 cgtgtcgcaa ctggcgatcg ttccgatgca ggatattctg gggctcgggc gcgggcagcg
     6301 catgaacacg ccgggcacga ccgagggaaa ctggtgttgg cgtttcgcct gggagcaact
     6361 gccgcctgac ttgccggaac gactgcggcg catggcgcgg atgtacggac gcacagatta
     6421 aatgactggg cgctattgtt tattgtttgc cgcttttttc ctgaggtgaa actgccgctt
     6481 ccgggtttgt aacacggctg acgacgtcta ttgagtctta agccgctctc gtggcgggtg
     6541 catctcctca agggggtgcg cccgattttt tatgtgtcag ttcagcgaat atccccgcgg
     6601 ttccaccggc gcactggaag gggcaggttg ttcatggatg
//
LOCUS       DBNGZE010000016         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1807653, whole genome shotgun sequence.
ACCESSION   DBNGZE010000016 DBNGZE010000000
VERSION     DBNGZE010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1807653"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..289)
                     /gene="tatA"
                     /locus_tag="AC16FV_00410"
     CDS             complement(38..289)
                     /gene="tatA"
                     /locus_tag="AC16FV_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HIR2848557.1"
                     /translation="MGTFSIWHWLVVLIIVLLIFGTKKLRNLGSDLGGAIKSFKQAMK
                     EDGEPGAPASGEGDEAKKIKNDSGRVIDAEVTSKQKHKT"
     gene            complement(360..683)
                     /locus_tag="AC16FV_00415"
     CDS             complement(360..683)
                     /locus_tag="AC16FV_00415"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR2848558.1"
                     /translation="MSDVLKKLAAVLEARKGADPQSSYVAGLYAKGLDAILKKIGEEA
                     TETVLAAKTGERAKIIHETADLWFHTLIMLAQTGLKPDDILAELDRRFGVSGLDEKAS
                     RQGRQ"
     gene            complement(685..1077)
                     /gene="hisI"
                     /locus_tag="AC16FV_00420"
     CDS             complement(685..1077)
                     /gene="hisI"
                     /locus_tag="AC16FV_00420"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR2848559.1"
                     /translation="MTESWLDAVKWNAEGLVPAIAQEAGSGKVLTLAWMNREALAATA
                     REKRAVYWSRSRRKLWRKGEESGHVQAVKDIRLDCDNDAVLLIVEQKGGIACHTGRER
                     CFYQQLQDGRWVTVEPVIKSEQEIYGRK"
     gene            complement(1133..1894)
                     /gene="hisF"
                     /locus_tag="AC16FV_00425"
     CDS             complement(1133..1894)
                     /gene="hisF"
                     /locus_tag="AC16FV_00425"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR2848560.1"
                     /translation="MGLAKRIIPCLDVDNGRVVKGVRFVEIRDAGDPVEIARRYDEQR
                     ADEITFLDLTASSDNRETLVHVVEAMASQVFIPLTVGGGIRKVEDVRRMLNAGADKVA
                     INTAAVSRPEFVKEASDHFGSQCIVVAIDAKQVGPDKWEVFTHGGRKATGLDAVAWAR
                     KMTEYGAGEILLTSMDRDGTKDGFDLKLTRAVCDAVSIPVIASGGVGNLDHLVEGITR
                     GHADAVLAASIFHFGEYTVAHAKQRMAEAGIEVRL"
     gene            complement(1993..>2411)
                     /locus_tag="AC16FV_00430"
     CDS             complement(1993..>2411)
                     /locus_tag="AC16FV_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR2848561.1"
                     /translation="NAPHFVSDVCTEFPGHIIVGLDARDGKVAIDGWSKLSGHDVVDL
                     AKKFQDDGVEAIIYTDIGRDGMMTGVNIEATIKLANAISIPVIASGGITDIEDIRKVC
                     EAGEAGIVGAITGRAIYEGTLDFAAAQKLADELGKK"
BASE COUNT          432 a          749 c          702 g          528 t
ORIGIN      
        1 ccgatgtcga acatcgtgcg tgatcgtggc gggaagttta ggtcttgtgt ttctgcttgg
       61 aagtgacttc cgcgtcaatc acgcggccgg aatcgttctt gatcttcttg gcctcgtcgc
      121 cctcaccgga cgccggcgcg ccaggttcgc cgtcttcctt catcgcctgc ttgaagctct
      181 tgatcgcgcc gcccaagtca ctgccaagat tgcgcaactt cttggtgccg aaaatcagca
      241 gaacgatgat cagtacgact agccagtgcc aaatgctgaa agtacccatg ctgtttctcc
      301 agaaaattcc gttcgtgtct tgccaatacc tgtacggcgg gtgcgtaatc gccgccgctc
      361 tactgacgtc cctggcgcga ggccttctcg tccagaccgg acacaccaaa acgccgatcc
      421 agttcagcta ggatatcatc cggcttcagt cccgtttgcg caagcataat cagcgtgtgg
      481 aaccacaggt cggcggtctc gtgaatgatt ttcgcgcgct cgccggtctt ggccgcaagc
      541 acggtttcgg ttgcctcttc cccgattttc ttgagaatcg cgtcgagccc cttggcgtat
      601 agtcccgcga cataggagct ctgcggatca gcgcccttgc gcgcttccaa aaccgcggcc
      661 agtttcttca ggacgtcgga catgtcattt cctgccgtag atttcctgtt cgcttttgat
      721 cacgggctcg accgtcaccc agcgcccatc ctgcaattgc tgataaaaac agcgctcgcg
      781 tccggtgtga caggcgatgc cgcccttttg ttcgacgatc agcagcacgg catcgttgtc
      841 gcagtcgagg cggatatcct tcaccgcctg cacgtgtccg gattcctcgc ccttgcgcca
      901 gagtttgcgg cgcgaacgcg accagtacac cgcgcgcttc tcccgcgccg tcgccgccag
      961 cgcctcgcga ttcatccacg cgagcgtcag caccttgccg ctgccggcct cctgcgcgat
     1021 cgcggggacc agaccttcgg cattccactt taccgcgtcg agccatgatt cagtcatggc
     1081 ggaataataa caggaattag gtgcgccgat gcgcggaggg ggttatggcc tactacagcc
     1141 ttacttcaat gccggcttca gccatgcgct gcttggcatg cgcaacggtg tattcaccga
     1201 agtggaaaat gctggccgcc agcacggcat cggcgtgacc ccgggtgatg ccctcgacca
     1261 ggtgatcgag attgccgaca ccaccggagg cgatcacggg aatgctgacg gcatcgcaca
     1321 cggcgcgtgt cagcttgagg tcgaacccgt ccttggtacc atcacggtcc atgctggtca
     1381 gcaggatttc gccagcgccg tattccgtca tttttcgggc ccaagccacg gcgtcgaggc
     1441 cggtggcctt gcgcccgcca tgcgtgaaca cctcccattt gtccggcccg acctgcttgg
     1501 cgtcgatggc gaccacgatg cactgcgatc cgaaatgatc cgaagcttcc ttgacgaact
     1561 cgggacgtga cacggcggcg gtgttgatcg cgaccttgtc ggcgccggca ttgagcatgc
     1621 ggcgtacatc ttcaaccttg cggatgccgc cgccgacggt gagtggaata aaaacctggc
     1681 tggccatggc ctcgaccacg tgcaccaggg tttcgcggtt gtcggaactg gcggtgaggt
     1741 cgaggaaggt gatctcgtcc gcgcgctgct cgtcgtagcg gcgcgcgatc tcgaccggat
     1801 cgccggcatc gcggatttcc acgaagcgca cgcccttgac gacgcgaccg ttgtccacgt
     1861 cgagacaggg aatgatgcgc tttgctaggc ccataagata tgagtatcac cgccagggcg
     1921 ccaggatcgc cagggaagcg aaaattagaa aacgtttttc ctggcgttct tggcgtcctg
     1981 gcggttcaga tattattttt taccgagctc atccgcaagc ttctgcgcgg cggcgaagtc
     2041 gagcgtacct tcatagatgg cgcggccggt gatggcaccg acgatgccgg cctcgccagc
     2101 ctcgcacacc ttgcggatgt cttcgatgtc ggtgatgcca ccagaagcga taaccgggat
     2161 ggaaatggcg ttcgccagtt ttatcgtggc ctcgatgttc acgccggtca tcatgccgtc
     2221 gcggccgata tcggtgtaga tgatggcttc gacgccgtca tcctggaatt tctttgccag
     2281 atcgacgacg tcgtgccccg agagttttga ccagccgtcg atagcgacct tgccgtccct
     2341 ggcgtcgagt ccgacgatga tgtggccggg gaattccgtg cacacgtcgc tcacgaaatg
     2401 cggggcgttc a
//
LOCUS       DBNGZE010000017         2479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1980, whole genome shotgun sequence.
ACCESSION   DBNGZE010000017 DBNGZE010000000
VERSION     DBNGZE010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1980"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..820
                     /gene="fnr"
                     /locus_tag="AC16FV_00435"
     CDS             59..820
                     /gene="fnr"
                     /locus_tag="AC16FV_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HIR2848562.1"
                     /translation="MQKREAAAIINISSIKAACKDCSLQELCLPLGLNEVDITALDKV
                     IKRRRTLKKGEMLYRFGDPLRALYAIRSGALKTTGLIEDGRAQVTGFYLPGELLGIDA
                     INSDKHPCSAEALESSEICEIPYPALEDLATKIPGLQHQLLRIMSREIVRDEEMLMML
                     GRMSADERLASCLMSFSRRLARLGAEPSEFKLSMSRQDLGDYLGLALETVSRLLSRFH
                     DEGLISVHGRQITLRNIGRLRSMAAGNGREHSASA"
     gene            853..1878
                     /gene="bioB"
                     /locus_tag="AC16FV_00440"
     CDS             853..1878
                     /gene="bioB"
                     /locus_tag="AC16FV_00440"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR2848563.1"
                     /translation="MTSRPACPSVPAFPGILPMRPETSEIADLFELPFNDLLYRAQSV
                     HRRHFDPNAVQLSTLLSIKTGGCSEDCGYCSQSARFHTDVPSQPLMSKDEVVAAARTA
                     KARGATRFCMGAAWRGPKEKELVQVTEMIRAVKNLGMETCATLGLLREGQAEQLAQAG
                     LDYYNHNLDTSRAHYDNIVHTHTFDSRLDTLKKVRSAGLKVCCGGIMGMGESRTDRVQ
                     FIAELAALDPPPESVPINLLVHIPGTPLSEKPTLDPIEFVRTIAVARIAMPTSFVRLS
                     AGRSAMPDHLQALCFLAGANSIFYGDRLLTTPNPESDADLRLFEKLGIHPLSAETPAP
                     QRKIAEA"
     gene            complement(1906..2466)
                     /locus_tag="AC16FV_00445"
     CDS             complement(1906..2466)
                     /locus_tag="AC16FV_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2848564.1"
                     /translation="MKICIVSDSHDHRDHLAAAVTEAKSLGVQAVIHCGDLVAPSTLH
                     AIIPLGLPVHLVHGNNMGDLFHLSKLAHKPENRVHYYGQDGSFTLAGKRIFLVHYPHY
                     AKAMAVTGDYDLVCNGHEHHAVIERIKNIKGNETLRIDPGTVGGVSAPATWVLGNLEK
                     MEFEIRAVRLPEPASGKQMDSKIARA"
BASE COUNT          474 a          790 c          720 g          495 t
ORIGIN      
        1 tggacgtacc ccggcaattt tgttacaaca agctcccaag gcataaggaa aaagccccgt
       61 gcaaaaacga gaagctgctg cgatcatcaa tatctccagc atcaaggcag cctgcaagga
      121 ttgctcgctc caggaattat gcctgcccct gggtctcaat gaggtcgata tcaccgccct
      181 cgacaaggtc atcaagcgcc ggcgtaccct caaaaagggc gagatgcttt accgttttgg
      241 cgatcccctg cgcgcgcttt acgcgatacg cagcggcgcg ctcaagacca ccggcctgat
      301 cgaggacggg cgcgcccagg tcaccggttt ttatctcccc ggcgaattgc tcggcatcga
      361 cgccatcaac tccgacaagc atccgtgcag cgccgaggcg cttgagagct cggagatttg
      421 cgagattccc tatcccgcgc tggaagactt ggcgaccaaa atccccggcc tgcaacatca
      481 attattgcgc attatgagtc gcgagatcgt gcgcgacgaa gaaatgctca tgatgctggg
      541 gcgtatgagc gcggatgaac gcctggcctc gtgcctcatg agtttttccc gccgcctggc
      601 gcggctcggc gccgaaccgt cggaattcaa gctcagcatg tcgcgccagg acctgggcga
      661 ttatctcggt ctcgcacttg agacggtgag ccgtctgttg tcgcgcttcc atgacgaagg
      721 tttgatcagc gtgcatggcc ggcagatcac gctgcgcaac atcggtcgcc tgcgttccat
      781 ggccgccggc aacggtcgcg agcacagcgc ctcggcctga ggtcttccgt caacccgcgt
      841 ttcgttacag ggttgacaag cagaccggct tgccctagcg tgccggcttt ccccggaata
      901 ttgccgatgc gtcccgagac ctccgaaatc gccgatctgt ttgagttgcc gttcaacgat
      961 cttctctatc gtgcccagtc cgtgcaccgc cggcatttcg atccgaacgc agtgcagctt
     1021 tccacgctgc tgtccatcaa gaccggcggt tgctccgagg attgtggcta ttgctcgcag
     1081 tcggcgcgct tccacaccga cgtgccaagc cagccgctga tgtcgaagga tgaagtggtc
     1141 gccgcggcgc gcacagccaa ggcgcgtggc gccacccgct tctgcatggg tgcggcctgg
     1201 cgcgggccga aggaaaaaga actggtgcag gtcacggaga tgatccgcgc ggtcaagaac
     1261 ctcggcatgg aaacctgtgc cacgctcgga ttgctgcgcg aaggccaggc cgaacagctg
     1321 gcgcaggcgg gactcgatta ttacaaccac aacctcgaca cctcgcgcgc ccattacgac
     1381 aacatcgtgc acacccatac cttcgacagc cgcctggata ccctgaagaa ggtgcgctcc
     1441 gccggactca aggtctgctg cggcggcatc atgggcatgg gggagtcgcg cacggatcgt
     1501 gttcagttca tcgccgaact ggctgcgctc gacccgccgc cggaatcggt gccgatcaac
     1561 ctgctggtgc acatccccgg aacgccgtta tccgaaaaac ccacgctcga ccccatcgaa
     1621 ttcgtgcgta ccatcgcggt cgcgcgcatc gccatgccga cgtcgttcgt gcggctgtcc
     1681 gccggccgca gcgcgatgcc ggaccacctc caggccctgt gttttctggc aggcgccaat
     1741 tccattttct acggcgaccg gctgctgacc acgcccaacc cggaatccga cgccgatctc
     1801 aggctgttcg aaaaactggg aattcacccc ttgtccgccg agacaccggc gccgcaacgc
     1861 aagatcgccg aggcctgaca gcttttcgct ccgggttacc ggggttcagg cgcgcgcgat
     1921 tttcgagtcc atctgtttgc ccgaggccgg ttccggcagg cgcacggcgc ggatttcaaa
     1981 ttccattttt tccagattgc caagcaccca ggtcgcgggc gcgctcacgc cgccgaccgt
     2041 gccgggatcg atgcgcagtg tttcattacc tttaatattt ttaatccgct caatgacggc
     2101 gtgatgttcg tggccgttgc acaccaggtc gtaatcgccg gtgactgcca tggccttggc
     2161 gtagtgcggg tagtgcacaa gaaatattcg ttttcctgcc agcgtgaatg acccgtcctg
     2221 accgtagtaa tgcacccggt tttccggctt gtgcgcgagt tttgaaagat gaaacaggtc
     2281 gcccatgttg ttgccgtgca ccagatgtac cggcagcccc agcgggatga tggcgtgcag
     2341 cgtggacggc gccaccagat cgccgcaatg aatcaccgcc tgcacgccca gcgatttggc
     2401 ctcggtcacg gccgcagcca gatggtcgcg atgatcgtgg ctgtcggaga cgatgcagat
     2461 tttcatgacg ctattgtat
//
LOCUS       DBNGZE010000018         1566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1391188, whole genome shotgun sequence.
ACCESSION   DBNGZE010000018 DBNGZE010000000
VERSION     DBNGZE010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1391188"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1440
                     /locus_tag="AC16FV_00450"
     CDS             211..1440
                     /locus_tag="AC16FV_00450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR2848565.1"
                     /translation="MILGFAGLLSLVGTDRAQAEAQLLIEASTGKVLHAENATYPWYP
                     ASVTKLMTAYTTLRAIKEGRISLNTLLTMSRNAAAQQPTKMGFSIGTTVTVDNALKML
                     MVKSANDIAVAIAEGVGGSIGGFADLMNANARRLGMSQSNFVNPNGLPAENHVTSARD
                     LAILARALIREFPEYDSFWHISSIRYGNRVMRNYNSLIDRYPGADGMKTGFICASGYN
                     VVASATRNGRRLIAVVLGSWSGAARAQKAAQLLERGFNSGGLSWLTPSLGTVDALAPI
                     DAQPPNLREEMCGGHRRKPPSEENDEEPEEAATATAASGESSNGQAFMLSNLKPATGK
                     FVLGPPVETTPPVVVFTGSADHPDPVAQTASAVPKKKKKVAAKKEVAEKPANTAKTAK
                     ATKPAKHTKPKVSSATQ"
     gene            1437..>1566
                     /locus_tag="AC16FV_00455"
     CDS             1437..>1566
                     /locus_tag="AC16FV_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR2848566.1"
                     /translation="MSESARTDRRAPPTPIPMTVLTGFLGAGKTTLLNRLLKDTALD"
BASE COUNT          344 a          501 c          433 g          288 t
ORIGIN      
        1 ggtcatcgat gcctcctttg ccgaactttg gtcgctttcc tcagcgatga gccgaatgtc
       61 cccatatact aagccgcggt tcgccgactt tgcctaccat ccaaagctta gatagcctag
      121 cgcggccaag catggaactt tcccgttttt gaggcttagg accctgaatc gcgaacgatg
      181 attgcttttt caagccaccg ctctcgcgcc atgattctcg gctttgccgg cttgctgtcg
      241 cttgtcggca cggaccgcgc acaagccgaa gcacaactgc tgatcgaggc ctctaccggc
      301 aaggttctgc atgcggaaaa tgccacgtat ccgtggtacc ccgcttcggt caccaaattg
      361 atgaccgctt acacgacgct ccgtgcgatc aaagaaggcc gcatctcgct caacacattg
      421 ctcactatgt cgcgcaacgc agcagcgcag cagccgacca aaatgggctt cagcatcggc
      481 accacggtga ccgtcgataa tgcgctcaag atgctaatgg taaaatccgc caatgacata
      541 gcggttgcaa tcgctgaggg cgtcggcggt tcgatcggag gcttcgcgga cctgatgaat
      601 gcgaacgccc ggcggcttgg catgtcgcaa agcaatttcg tcaacccgaa cggattgccg
      661 gcggaaaatc acgttacgtc cgcgcgtgat ctcgcaatat tggcgcgcgc gctcattcgc
      721 gagtttccgg aatacgacag tttttggcat atctcgtcga tccgttacgg caatcgcgtg
      781 atgcgcaatt acaactcgct gatcgatcgt tatcccggtg ccgacggcat gaagaccgga
      841 ttcatttgcg cctctggcta caacgtcgtt gcctccgcca cgcgcaacgg gcgccggctc
      901 atcgcggtcg tgcttggctc ctggtcaggt gccgcacgcg cgcaaaaggc ggcccagctg
      961 ctcgaacgcg gtttcaacag cggcggcttg tcctggctca caccctcgtt gggcaccgtc
     1021 gatgcgttgg cgccaattga tgcacagccg cccaacttgc gcgaggaaat gtgcggaggc
     1081 catcgccgca agccgccctc agaagaaaac gacgaggaac ctgaagaagc ggccacggcc
     1141 accgccgcca gcggcgaatc gagcaatggc caggccttca tgctctccaa cctgaagccg
     1201 gccaccggca agtttgtgct cgggccgccg gtcgagacca caccgccggt cgttgtattt
     1261 actggatcag ccgaccatcc cgatccagtc gcgcagactg cgagcgccgt gccgaaaaag
     1321 aaaaagaaag tcgccgccaa gaaggaagtg gccgaaaagc cggcgaatac cgccaagaca
     1381 gctaaggcca ccaagccggc aaagcacacc aaaccgaaag tcagctcggc gacgcaatga
     1441 gcgagtccgc tcgcactgac cgaagagccc cgccgacgcc aatcccaatg acggtgctca
     1501 ccggcttcct cggcgccggc aaaactacat tgctcaatcg gctgttgaag gacaccgcgc
     1561 tcgacg
//
LOCUS       DBNGZE010000019        17530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1877602, whole genome shotgun sequence.
ACCESSION   DBNGZE010000019 DBNGZE010000000
VERSION     DBNGZE010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 17530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17530
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1877602"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16FV_00460"
     CDS             complement(<1..418)
                     /locus_tag="AC16FV_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR2848567.1"
                     /translation="MIQVSQTAESVASGSMELSSAAEQLSSSAQEQASSLEETAASME
                     EMTSTVKQNAYNALRADKVALDAREAANEGVVMSSSVKRSMEAINSSSTKIADIIGVI
                     DEIAFQTNLLALNAAVEAARAGEQGRGFAVVASEVRN"
     gene            complement(485..865)
                     /locus_tag="AC16FV_00465"
                     /pseudo
     CDS             complement(485..865)
                     /locus_tag="AC16FV_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            complement(898..1410)
                     /locus_tag="AC16FV_00470"
     CDS             complement(898..1410)
                     /locus_tag="AC16FV_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR2848568.1"
                     /translation="MQTAHAMEQNGMRTGDEQFLTFRLHGQDYGITIHKIQEIKGWDK
                     MTPIPNSPAYIKGVLNLRGVIVPVIDLRLRFGLPEAERDVFTVIIVANVNGRLAGIVV
                     DAVSDVINLGAEQLCDAPEYEGQHNREFIKGLAQVDNKLLVLLDIDRLVTPDSLDNSA
                     AASAPATAAR"
     gene            complement(1421..3448)
                     /locus_tag="AC16FV_00475"
     CDS             complement(1421..3448)
                     /locus_tag="AC16FV_00475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HIR2848569.1"
                     /translation="MAIDMRQFHQTFFEESLEGLAGMETELLHLEKAAANNGQATVAA
                     DPEILNTIFRAAHSIKGGSSTFGFEDVAAFAHVLESRLDALRDGRSRPDRRVISLLLL
                     SVDCLRSLIISARTGKEADINAIDSVRVQLECMESESSASVAPTMAISQTRTSATAHW
                     HIVFRPYRRLFHTGNDPLRILRELSDLGEIRVQADLAELPAWEQMDPEECYLAWNIEL
                     IGPVSREAIAEVFSWVMDDCVLELNEKAVQSLAPRSIAEAVASMDAHADTLRVSVPKV
                     DALINTVGELVITQTMLSQLTTNFSFDNLSRLFAGIAQLERNTRELQENVMHIRMLPL
                     SFAFNRLPRLVHDMGQKLEKKVELIIHGEQTEIDKTVIDRLIDPLLHLVRNSLDHGIE
                     RPAERVTAGKPEIGRIELKAYQKGGNVVIEIHDDGRGLSYDRIFSKAVERGLIKASAK
                     PSPEEIIEMIFMPGFSTAETVSDVSGRGVGMDVVRNNIRSLGGTVEVFSQKGHGTCFT
                     VRLPLTLAILDGLSVQVGAHTYILPLVSIVESVRINPEQVTRPAGGAELYGLRKEYLP
                     LLRLYELFHIEPRSADLAQGLLVIVEADGKKAGLYVDDLLGQQQVVIKSLATHYRKVE
                     GISAATILGDGTVAMILDVTGLTRLAHAGAGRASIPAGDASMTTAITPTLN"
     gene            complement(3459..3821)
                     /locus_tag="AC16FV_00480"
     CDS             complement(3459..3821)
                     /locus_tag="AC16FV_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2848570.1"
                     /translation="MATILTVDDTASMRQMISFTLNSVGHEVIQASDGKEALKLLEGK
                     KVDLVIADVNMPNMDGITLVKSLREQADYKFTPILMLTTESQESKRQQGKVAGATGWI
                     VKPFNPEQLLNVVKKVLG"
     gene            complement(3834..5213)
                     /locus_tag="AC16FV_00485"
     CDS             complement(3834..5213)
                     /locus_tag="AC16FV_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR2848571.1"
                     /translation="MNGSFSFKLSASGKTGAWSRWWLWAAVGMTLSLMWTVAFVPWWA
                     ALGSGLSAVLTLTVLAFYARSQDEATPLQGEEVLEGHDAKHVLEQVDDLMSSSKDIEQ
                     IPQVDEVLQTMAADNQRRRLDDLMRRWVDVGQRYAEAALVLRREIQAVIKQTEQATAT
                     IGSSFEAVINKATIQARQAMELLEGTQGAASDGVPQSLKDFIQVSDERLNKMADEVVR
                     VADLSVNMVRELDGVQNRTQAIDGFLLDVEKLADQTSLLALNADIEAARVGEHGRGFS
                     VVASEVRRLSKRSHEFSDRIRVHLKEVRTGLSKTYGDMRTLSAADMEHALKIKDDVIT
                     LTRSLEGKNREVAETVSRINEISKEIAQDVQNVVVSLQFQDITSQKLSGMLEPMDDLR
                     RTLYHLMQETVNLDKTLLKNLPGNENWMSRMRDVPAAEAEVKLTKNPEQDLRKVHGAM
                     GNGPAVELF"
     gene            complement(5210..5527)
                     /locus_tag="AC16FV_00490"
     CDS             complement(5210..5527)
                     /locus_tag="AC16FV_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR2848572.1"
                     /translation="MGNSMDPVHIEHTADTTHVVLEAALGIRDARVLHEKLGTVLAAA
                     TAVVLDGSRVERLDAAAMQVLAGFTRAAREQGLALTWQSPSPVLQQAARVLGFESILE
                     MAS"
     gene            complement(5811..6893)
                     /gene="thrC"
                     /locus_tag="AC16FV_00495"
     CDS             complement(5811..6893)
                     /gene="thrC"
                     /locus_tag="AC16FV_00495"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR2848573.1"
                     /translation="MNIGNRYTGLIEKYRDRLPVSQNTRIISLGEGATPLIQLRNIPR
                     ELGRQVDIYVKFEGLNPTGSFKDRGMTMAVTKAVEEGSRAIICASTGNTSAAAAAYAA
                     RAGITCFVVIPDGKIALGKLAQAMIHGAVVIQIQGNFDAGMELVKQVAHEAPVTIVNS
                     INPYRLQGQKTAAFEIVEELGSAPDYHCLPVGNAGNITANWMGYCEYADAGICRTRPV
                     MVGYQAAGAAPFLLGKMVDNPETIATAIRIGHPQSWDKAWQVQKESKGWFDKFTDEEI
                     LAAQKLLSQKEGVFCEPASATSVAGAMRDIKSGKIPEGSRVVCTLTGHGLKDPDTAIK
                     QSSGPLHKVKAELGAVKKTILDNLPR"
     gene            complement(7037..8359)
                     /locus_tag="AC16FV_00500"
     CDS             complement(7037..8359)
                     /locus_tag="AC16FV_00500"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR2848574.1"
                     /translation="MKPVKIGILGLGTVGCGTVSVLARNAQEISRRAGREIRIAQAAA
                     RDLGKKRSCPMNGIQLVQDPYEVVRNPEIEVVVELIGGYEPAHELVLAAINNGKHVVT
                     ANKALIAMHGNEIFAQAQKKGVMVAFEAAVAGGIPIIKAVREGLAGNRIEWMAGIING
                     TCNFILTEMRDKGADFADVLKEAQRLGYAEADPTFDVEGIDAAHKLTILAAIAFGIPL
                     KFKQAYTEGITKLTRMDVQYAEKLGYRLKLLGIARRTNGGVELRVHPTLIPERRLIAN
                     VDGAMNAVLVKGDAVGPTLFYGAGAGAEPTASAVVADLVDVVRTLTADPNNRVPHLAF
                     QPDALVDLPILPIEEVETSYYLRMHALDKPGVLAEVTRILADLGISIESILQKEPQTG
                     EETVPVIILTQRIKEKNMNEAIARIEKLASIKGKVARIRLEHLNPDWK"
     gene            complement(8487..9704)
                     /gene="alaC"
                     /locus_tag="AC16FV_00505"
     CDS             complement(8487..9704)
                     /gene="alaC"
                     /locus_tag="AC16FV_00505"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR2848575.1"
                     /translation="MSQNDEFPRIKRLPPYVFNIVNELKAQARRRGEDIIDFGMGNPD
                     GATPKHIVDKLVEAAQREDTHRYSVSRGIPRLRRAICQWYKRRYDVDLDMDSEAIVTI
                     GSKEGLSHLALATVDKGDSVLVPNPSYPIHAYGFVIAGADIRHVPLVPGVDFFAELEK
                     SIKNAWPKPKMLVLNFPANPTTQCVELDFFEKVIAIAKEHGIWVVHDLAYADIVFDGY
                     RAPSILQVPGAKDVAVEFFTLSKSYNMPGWRIGFMCGNQKLVAALSRLKSYFDYGMFT
                     PIQIAAILALEGPQDCLEEIRQTYQNRRDVLCNGLFGAGWLVEKPKATMFVWARIPEA
                     YRAMGSIEFSKKLLTEAKVAVSPGIGFGEYGDDHVRFSLIENEHRTRQAIRSIREMFR
                     KDKVSFDVRQVKN"
     gene            9823..10215
                     /locus_tag="AC16FV_00510"
     CDS             9823..10215
                     /locus_tag="AC16FV_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR2848576.1"
                     /translation="MKIHLESGTGQNLIRAYAPGSITINDVAYTRSLIVTPEQIIDWP
                     PASFAEILAVHFDVLAAMRPEVVILGTGAKLQFPSPVLTRALVDENIGLEVMDTGAAC
                     RTYNILMSDGRRVAAALLMIENLKQGID"
     gene            complement(10195..11166)
                     /gene="rpoS"
                     /locus_tag="AC16FV_00515"
     CDS             complement(10195..11166)
                     /gene="rpoS"
                     /locus_tag="AC16FV_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HIR2848577.1"
                     /translation="MEDETLVAAEDSEDEETETAEPAAEVASKEDEPEAEASESDKRD
                     AYYSHADATRIYLKEIGFSPLLSAEEEVFFARKAQKGDGKARKRMIESNLRLVVKIAR
                     RYMNRGLSLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERGIMNQTRTI
                     RLPVHVLKEINIYQRAARYLAQKLDHEPSPEEVANLLDKPVEDVKGMLGLNERVASVD
                     APLDDDPDRSLLDAIADERTPDPEKVLQRDDLQQLIQQWLNELNDKQREVVERRFGLN
                     HREISTLEEVGADIGVTRERVRQIQVEALKRLRVILEKAGYSVDSLF"
     gene            complement(11268..12029)
                     /locus_tag="AC16FV_00520"
     CDS             complement(11268..12029)
                     /locus_tag="AC16FV_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR2848578.1"
                     /translation="MNSCLKICGIIMLAIGISACGSFPLAPVSEQGRELRQPEAGYRV
                     VQPGDTLYSIAWESGRDYQDVARWNQIPPPYLIKPGQRLRLYPPTGVPKARRPEPSAS
                     SKPEIHKKQAPDKPEPTAPVRHAPVAAAKQLSWAWPAQGELLERYSANGPNKGIDIAG
                     KKGQPILAAEAGQVVYQGSGLRGYGQLIIVKHNADFLSAYAHCDKIYVKEGNVIKRGQ
                     KIADMGSSGTDRTKLHFEVRYRGAPVDPQDYLPRK"
     gene            complement(12061..12714)
                     /locus_tag="AC16FV_00525"
     CDS             complement(12061..12714)
                     /locus_tag="AC16FV_00525"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR2848579.1"
                     /translation="MDVHGIGMTSRRTRDRLIQRLREEGIRDERVLEAIRNVPRHVFV
                     DEALASRAYEDTALPIGHGQTISQPYIVALMTQTLLAGGRLKKVLEVGTGSGYQTAVL
                     AGLVDEIYSVERLEPLMKLARKRLREIGCRNVHIKLSDGSWGWKEHAPYDGIIVTAAP
                     AEVPPALLEQLAPGGRLIIPVGGPTMQELLLVQHTDKGLIQERLELVNFVPLVRDSL"
     gene            complement(12748..13494)
                     /gene="surE"
                     /locus_tag="AC16FV_00530"
     CDS             complement(12748..13494)
                     /gene="surE"
                     /locus_tag="AC16FV_00530"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR2848580.1"
                     /translation="MRILLSNDDGYLAPGLACLAKALEQVAQVDVVAPERDRSGASNS
                     LTLTHPLRVQKAGNGFYFVDGTPTDCVHLAITGLLEREPDIVIAGINRGANLGDDVIY
                     SGTVAAAMEGRFLGLPAIAVSLAGKVGAHFETAAHVTLRLLEKLQRESLPADTILNVN
                     VPDVPLEQLAGFEATRLGHRHKAEPVIRTSDPHGNPVYWVGAPGAESDAGPGTDFHAI
                     RNQFVSITPLHVDLTRYTALDQVSAWLRKI"
     gene            complement(13888..13964)
                     /locus_tag="AC16FV_00535"
     tRNA            complement(13888..13964)
                     /locus_tag="AC16FV_00535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13928..13930),aa:Pro,seq:ggg)
     gene            complement(14055..14414)
                     /locus_tag="AC16FV_00540"
     CDS             complement(14055..14414)
                     /locus_tag="AC16FV_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR2848581.1"
                     /translation="MLDPGENHELPTIPGKRYFTIGEVSDLCAVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRHDVILIRQIRSLLYVEGFTISGARNKLELDNPAPPDANRQQAI
                     KTLINELEDVLDMLKKL"
     gene            complement(14407..14739)
                     /locus_tag="AC16FV_00545"
     CDS             complement(14407..14739)
                     /locus_tag="AC16FV_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR2848582.1"
                     /translation="MALTKADLAENLFNELGLNKREAKEFVEIFFERIRGALSEGEQV
                     KLSGFGNFSLRQKNSRPGRNPKTGEEIPITARRVVTFRASHKLKERVEQNASANREKF
                     KQQAELNA"
     gene            complement(14783..17161)
                     /gene="pheT"
                     /locus_tag="AC16FV_00550"
     CDS             complement(14783..17161)
                     /gene="pheT"
                     /locus_tag="AC16FV_00550"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR2848583.1"
                     /translation="MKVSEQWLREWVSPRLDTRALAHRLTMAGLEVGAVEPVAPKLDK
                     VIVGKIISIAPHPSADKLRLCRVDIGKDKILDIVCGAANATVGMRAPVALEGAKLPNG
                     ILIKRTEIRGAASSGMLCSAQELGLAESAEGLLALDADAKPGTSLTEYLCLDDVSLEV
                     DLTPNRGDCLSVAGLARELAAITSVKMKSPAIKPVKPKHRQRVAVKLDAQQDCPHYIG
                     RVVTGINPHAVTPIWMKEKLRRSGIRSIHPVVDITNYVMIELGQPMHAFDLSKIRGSI
                     HVRHAAKNESLVLLDGNRLTPKPGSLMIADDAQPLALAGIMGGMDSAVSDATRDIFLE
                     SAWFRPETISIRSREHGLQTESSFRFERGVNPALQRLTMERATALLLSVVGGRPGPLV
                     EQTVKRHLPRVSPVTLRLERVSRLLGLDIPVKEVEDILKRLGMKLRKAGNKWAITPPS
                     HRFDISREVDLIEEIARIHGYDRLPSRRPRMDMAASPVPESDIGESRLRAGLIDRGYQ
                     EVVTYSFVDPGIQTLIDPQLSPARLANPISAEMAVMRTSLWPGLLQTILYNQNRQQTR
                     ARFFEIGRTFLPQGQDFSQEPMLAGAACGEAMAEQWGLPRRAVDFHDVKADVEALLVL
                     AGIGQGVRFQPGQHPALHPGQTAEIRLDDNQIGLVGVLHPATQSRLGLDRPVVLFEIK
                     VGALRSGKIPNFREFSRFPSIRRDLAILVKDSTPAQAVLDCVQQAAGGLLVNLELFDE
                     YRGKGIDSGRKSLALGLTLQDSSRTLNEDDVERVMTQVMSALKSGLGAQPRQ"
     gene            complement(17259..>17530)
                     /locus_tag="AC16FV_00555"
     CDS             complement(17259..>17530)
                     /locus_tag="AC16FV_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA ligase subunit PheS family protein"
                     /protein_id="HIR2848584.1"
                     /translation="FTEPSAEVDISCVICGAKGCRVCKNTGWLEVLGCGMVHPEVFRH
                     VGIDSERWTGFAFGLGVERLAMLRYGVNDLRLFYENDLRFLQQFR"
BASE COUNT         3299 a         5212 c         5151 g         3868 t
ORIGIN      
        1 ggttacgcac ctcggacgcc accacggcaa aaccgcgccc ctgctcgccg gcgcgcgcgg
       61 cttcgaccgc cgcattcagt gccagcaggt tggtctgaaa cgcaatctcg tcgatcacgc
      121 cgatgatgtc ggcgatcttg gtgctgctgg agttgatggc ttccatcgag cgcttgaccg
      181 aactcgacat caccacgcct tcgtttgccg cctcgcgtgc atccagcgcg accttgtcgg
      241 cacgcagggc gttgtaggcg ttctgcttca cggtactggt catttcctcc atggaagcgg
      301 cggtttcttc gagcgagcta gcctgttcct gcgccgagga ggacagctgt tcggccgccg
      361 agctcagttc catggatccg ctggccacag actcggctgt ctgcgaaacc tggatcatgc
      421 tttcgtggaa ttcatccacc agcacgttaa gcgcgccgat ggcctgaccg acctcgtccc
      481 ttctgtcggt atagagcgtg gcgctaagat cgccctccgc tacccgctgc atggtgccaa
      541 cgaggcgatt caacggatcg gaaaccgtgc gcacaaaata gatcgcaatg gcgatgggga
      601 cgatgatcgc cgccaggatg ccgccgatgg tgatgatctg gtattgcctg tataccttct
      661 gtgtttgctc atggtgagcc tgcaaggttt tagcaagcgc ggaattgtca ttggcgacag
      721 tctgtgcctc ctgaatggag tccatcacct gtgacagact tcgcaatcca agtccaccga
      781 cggcggccag catgagtgtc atgatcagcg cggcgccaat cattttgatt cggaggttaa
      841 ggccctttgc atagtacatt tccatgacat gctcctgttg cgcgaaaatt ttccgtatta
      901 ccgtgccgct gtggcgggtg ccgaggccgc tgctgaattg tccaatgaat ccggggtgac
      961 caggcggtcg atgtccagca ggacgagcag cttgttgtca acctgcgcca gccccttgat
     1021 gaactcacgg ttgtgctgac cctcgtattc gggcgcatcg cacaattgct cggccccgag
     1081 attgatcacg tcactcacgg cgtcgacgac gatgcccgcc agacggccgt tgacattggc
     1141 cacgatgatg accgtgaaga catcgcgctc ggcctccggg aggccgaatc gcaggcgcaa
     1201 atcgatcacc ggtacgatca cgccgcgcag gttaagcacg cccttgatgt aggccgggct
     1261 gttcgggatc ggcgtcatct tgtcccagcc cttgatttcc tggatcttgt ggatggtgat
     1321 gccgtaatcc tgaccgtgca gacgaaaggt caggaactgt tcgtcgccgg ttcgcatccc
     1381 gttttgttcc attgcgtgcg ccgtttgcat gtctctttcc tcagttcaaa gttggtgtaa
     1441 tcgccgtggt catggacgcg tcaccggccg gaattgacgc gcgcccggcg ccggcatgcg
     1501 ccaggcgagt caggccggtc acgtccagaa tcatcgccac ggtgccgtct cccagaatcg
     1561 ttgccgcgga aatgccttcg accttgcgat agtgcgtcgc cagacttttg ataacgacct
     1621 gttgctggcc gagcagatcg tccacgtaca gccccgcctt cttgccgtcc gcctccacga
     1681 tcaccagcaa gccctgggca agatcggccg aacgcggctc gatatgaaac agctcataaa
     1741 gccgcagcag cggcaagtat tccttgcgca gcccgtacag ttcggcgcca cccgcgggtc
     1801 gggtgacctg ctccgggttg atgcgcactg attcgacaat gctgacgagt ggcagaatgt
     1861 aggtatgggc gcccacctgc acgctcaagc catccagaat ggccagggtc aagggcaggc
     1921 gcacggtgaa gcaggtaccg tgtccttttt gcgagaagac ctcgacggtg cccccgaggc
     1981 tgcggatgtt gttgcgcacc acgtccatgc ccacgccacg gccggaaaca tcgctgacgg
     2041 tttccgccgt cgaaaatccc ggcatgaata tcatttcaat gatttcctcc ggtgagggtt
     2101 tcgccgacgc cttaatcagg ccgcgttcca cggccttgct gaatatgcgg tcgtacgaca
     2161 ggccgcggcc atcgtcatgg atttcgatga cgacattgcc gcccttctgg taggccttca
     2221 gttcgatgcg ccctatctcg ggtttgccgg cggtgacgcg ctccgccggg cgctcgatgc
     2281 catggtccag gctgttgcgc accagatgca acagcggatc aatcagacgg tcgatcacgg
     2341 tcttgtcgat ttccgtctgc tcgccgtgaa tgatcagctc cactttcttt tccagcttct
     2401 gccccatgtc gtgcaccagc cgcggcaaac ggttaaacgc gaagctgagt ggcagcatgc
     2461 ggatatgcat gacgttttcc tgcaattcac gggtgttgcg ttccagttgc gcgatgccgg
     2521 cgaaaagccg ggataggttg tcgaaggaga aattggtcgt gagctggctc aacatggttt
     2581 gcgtgatcac cagctctccg acggtattga tcagggcgtc aaccttgggc acgctcacgc
     2641 gcagcgtatc ggcgtgtgca tccatggagg cgaccgcctc ggcgatggag cgaggggcca
     2701 gggactgcac cgccttctcg ttcagttcaa gaacgcaatc gtccatgacc cagctgaata
     2761 cctcggcgat ggcctcgcgc gagaccggcc cgatcagctc aatattccag gcaagataac
     2821 attcctcggg atccatttgc tcccaggccg gtagctcggc cagatcggcc tgcaccctga
     2881 tctcgcccag atcgctcaat tcacgcagga tccgcaacgg atcgttcccg gtatgaaaca
     2941 ggcgccggta aggacggaac acaatatgcc aatgagctgt ggctgaagtc cgggtttggg
     3001 atattgccat cgttggtgcg acggaagcgc tgctttcgct ttccatgcat tccagctgga
     3061 cgcgcacact gtcaatggca tttatgtcgg cttcctttcc ggttctggcg gagatgatca
     3121 ggctgcgcag acaatcgacc gagagcagca ggaggctgat cacgcgccgg tccgggcgcg
     3181 aacgcccgtc gcgcagcgcg tcgagccgtg actcgagcac gtgcgcgaag gcagccacgt
     3241 cctcgaaacc gaaggtgctg ctgccgccct tgatggaatg ggccgcgcgg aagatggtat
     3301 tgaggatttc cggatcggcc gccacggtag cctggccgtt gttggccgcg gccttttcca
     3361 gatgcagcaa ctccgtttcc atgcctgcca gaccttcgag gctctcctcg aagaaggtct
     3421 ggtggaattg gcgcatatcg atggccatgg ccctatcctt atcccaggac ttttttgacc
     3481 acgttcagca gctgttccgg attgaaaggt ttgacgatcc agccggtggc gccggcgacc
     3541 tttccctgtt gccgcttgga ctcctgcgac tccgtggtga gcatcaggat tggagtaaat
     3601 ttgtaatctg cttgttcgcg caatgacttg acgagcgtga tgccgtccat gttaggcatg
     3661 ttgacgtccg ctatcaccag atccactttc ttgccctcga gaagcttgag cgcctctttg
     3721 ccgtctgaag cctggatgac ctcgtgcccc acgctgttca gcgtgaaact gatcatctgg
     3781 cgcatggaag cggtatcgtc aacggtcagt atggttgcca taacagtcct ccttcagaac
     3841 agttccacgg cgggtccgtt acccatggcc ccgtgaacct tgcgcaggtc ctgttccggg
     3901 tttttcgtca gcttgacctc cgcttcagcg gcggggacat cgcgcatgcg cgacatccag
     3961 ttctcgttgc ccggcaggtt tttgagcagg gtcttgtcca gattgacggt ttcctgcatc
     4021 aggtggtaca gcgtgcggcg caaatcatcc atcggctcca gcattccgct cagtttctgg
     4081 ctggtgatat cctgaaactg gagggagacc acgacgttct gcacgtcctg ggcgatttcc
     4141 ttgctgatct cgttgatgcg gctcacggtt tccgccactt cgcggttctt gccctcgagc
     4201 gagcgcgtca gcgtgatcac gtcatctttt atcttgaggg cgtgttccat gtcggcggcc
     4261 gacagtgtgc gcatgtcacc ataggtcttg ctcagaccgg ttcttacttc cttcaggtgc
     4321 acgcggatac ggtcgctgaa ttcgtgtgag cgtttgctga gtcgacgcac ttccgaggct
     4381 accaccgaga aaccacgccc atgttcgccg acgcgcgcgg cttcgatgtc ggcgttcagt
     4441 gccagcagac tggtctggtc cgccagcttt tccacgtcga gcaggaagcc gtcgatcgcc
     4501 tgcgtgcggt tctggacgcc atcaagttcg cgcaccatgt tgacggaaag gtctgcaacg
     4561 cgcaccactt catccgccat cttgttgagg cgttcgtccg aaacctggat gaagtctttc
     4621 aaggattggg gcacgccatc gctcgcggcg ccctgcgtgc cttcgagcag ctccatggcc
     4681 tgccgcgcct ggatggtggc cttgttgata accgcctcga aactgctgcc gatggtggcg
     4741 gtcgcctgtt ccgtttgctt gatgacggct tgtatttcgc gacgcaatac cagagcagct
     4801 tccgcgtaac gctggcccac atcgacccag cgacgcatca gatcgtcgag gcgacggcgt
     4861 tggttgtccg ccgccatggt ttgcaggact tcatccacct gtggaatctg ttcgatgtcc
     4921 ttggacgatg acatcaagtc atcaacctgt tcgagcacat gtttggcgtc gtgcccttcc
     4981 agaacttcct cgccctgcag cggtgtggct tcgtcctgac tgcgtgcgta gaacgccagc
     5041 acggtgagcg tgagcacggc gctcagcccg ctgcccagcg ccgcccacca tggcacaaac
     5101 gctaccgtcc acatcagcga gagagtcatg ccgacggcgg cccacaacca ccagcgtgac
     5161 caagcgccgg tttttcccga ggcggacagt ttgaatgaga aggatccgtt catgaggcca
     5221 tctcgagaat ggattcaaag ccgagcacgc gcgcggcctg ttgtaatacg ggggacgggc
     5281 tttgccaggt gagtgcgagc ccctgttccc gggcggcgcg ggtgaatcca gccagcactt
     5341 gcatggcggc agcatcgaga cgttcgacac ggcttccgtc gagcaccact gcggtggcag
     5401 cggccagcac ggtgcccagt ttttcgtgca gcacgcgggc gtccctgatg ccgagcgccg
     5461 cctccagcac cacgtgcgtg gtgtcggcgg tgtgctcgat atgtaccgga tccatgctgt
     5521 ttcccatttc gcccctcctt gccggggcct gcatccgcgt cggtttccat ggcccggggg
     5581 tcaccgggct tgctccttat aggtatagca atacctgtgc cagccttggg taaaaagcgt
     5641 ggaaatcaac gggaaacagg ccggggaagg caaaattggc ctgactgggc cgtaatcagg
     5701 gcatacccgt gcccggccct gctttcagag cagagcacgg aaacgcttgg ggacaccggt
     5761 ctggaatgcc ctgtctgggc aggatttaca gggaacgtca gttagcgggg ttagcgcggc
     5821 aaattgtcca ggatagtctt tttgacggcc cccaattccg ctttaacctt gtgcagcggg
     5881 ccgctgcttt gcttgatggc ggtatcgggg tccttgaggc cgtggccggt cagagtacag
     5941 acgacgcggc tgccttcggg aatcttgccg gacttgatgt cgcgcatggc accggcgacc
     6001 gatgtggcgg aagccggttc gcagaaaacg ccttctttct gcgacagcag tttttgcgcc
     6061 gcgagaattt cctcgtcggt gaatttgtcg aaccagccct tggattcctt ctgcacctgc
     6121 caggccttgt cccaagactg tgggtggccg atgcggattg cggtggcgat ggtctcgggg
     6181 ttgtccacca tcttgccgag caggaatggt gccgcaccgg ccgcctgata gccgaccatc
     6241 accggacggg tgcggcagat gccggcatcg gcatattcgc agtagcccat ccagttggcg
     6301 gtgatgttgc cggcgttgcc gaccgggagg caatgataat ccggcgcgct gcccagctcc
     6361 tcgacgatct cgaacgccgc ggttttctgc ccctgcaagc ggtaggggtt gatggagttg
     6421 acgatcgtga ccggcgcctc gtgggctacc tgtttgacga gttccatgcc ggcgtcgaaa
     6481 ttaccctgaa tctggattac gacggcgccg tgaatcatcg cctgcgccag tttgcccagt
     6541 gcgatcttgc cgtccgggat gaccacgaaa caggtgatgc cggcgcgcgc agcataggct
     6601 gccgccgcgg ccgaggtgtt gccagtggaa gcacagataa tggcacggct accctcttcc
     6661 accgccttgg tcacggccat ggtcatgccg cgatccttga aggaacccgt cggattaagt
     6721 ccttcgaact tgacgtagat atccacctgc ctgcccagct cgcgcggaat gtttctgagc
     6781 tgaattaaag gcgttgcacc ctcgcccaga ctgatgatgc gggtgttctg cgagaccggc
     6841 aggcggtcgc ggtatttttc gatgaggccg gtatagcggt tgccgatatt catgtgaatt
     6901 ttctataaga ttttggaacg cagattaacg cagatgatta aagatgatga acgcagataa
     6961 ttccgtggaa ttaagtcatc atttaaccaa tttgcgttta tctgcgcctt tatccgcgtt
     7021 catctgcgga taaaaattat ttccagtcag ggttcaggtg ctcaaggcgg atgcgcgcaa
     7081 ccttgccctt gatcgaggcg agtttctcaa tgcgggcaat tgcctcgttc atgttctttt
     7141 ccttgatgcg ctgcgtcagg atgatgacag gcaccgtttc ctcgcctgtc tgcggttcct
     7201 tttgcaggat ggactcgata ctgatgccaa gatctgccag gatgcgcgtg acctcggcaa
     7261 gcacgccggg tttgtccagc gcgtgcatgc gcaggtaata cgaggtctct acctcttcga
     7321 tcggcaggat cggcagatcg accagcgcat ccggctgaaa tgccaggtgt ggcacgcggt
     7381 tgttcgggtc ggcggtcagg gtgcgtacca cgtcgaccag atcggcgacc acggccgaag
     7441 ccgtgggctc ggcacccgcg ccggcaccgt aaaacagggt cggccccacg gcatcaccct
     7501 tgaccagtac agcgttcatg gccccatcca cgttggcgat cagccggcgc tccgggatga
     7561 gcgtggggtg cactcgcagc tccactccgc cgttggtacg gcgggcaatg cccagaagct
     7621 tcaggcgata gcccagcttt tccgcgtact gtacatccat gcgcgtgagt ttggtgatgc
     7681 cttcggtata ggcctgcttg aacttgagcg gaatgccgaa ggcgatggcg gccagaatgg
     7741 taagcttgtg cgccgcatca atgccttcga cgtcgaacgt cggatcggcc tcggcataac
     7801 ccaggcgctg cgcctccttg agcacatcgg caaagtccgc gcccttgtcg cgcatctccg
     7861 tcaggatgaa attgcaggtg ccgttgatga tgccggccat ccactcgatg cggttgcccg
     7921 cgagcccttc gcgcaccgcc ttgatgatgg ggatgccgcc ggcgaccgcg gcctcgaagg
     7981 ccaccatgac gccttttttc tgggcctgcg cgaagatttc gttgccgtgc atggcaataa
     8041 gcgccttgtt cgcggtgacc acatgcttgc cattattgat cgcggccaac accagttcgt
     8101 gcgccggttc ataaccaccg atcagctcta ccaccacctc gatttcggga ttgcgtacca
     8161 cctcatacgg gtcctgaacc agctgaatcc cattcatcgg gcaggagcgc tttttcccga
     8221 ggtcgcgtgc cgcggcttgg gcgatccgga tttcgcgtcc cgcacgacgg gagatttcct
     8281 gtgcattgcg cgcgagcaca ctgaccgtgc cacagcccac agtgccgaga ccgagaatgc
     8341 cgattttgac tggtttcata ttagtaatat ttttggaacc gcagattaac gcagatgaac
     8401 gcggattgaa aaagcaaatt tcacagttct ggtttttatc tgcgtttatc tgcgttcatc
     8461 agcggtttca ttaattatta aaattatcag tttttcacct ggcgtacgtc gaatgagacc
     8521 ttgtccttgc ggaacatttc acgaatggag cggatagcct gacgcgtgcg gtgttcgttt
     8581 tcgatgagcg agaaacgcac atgatcgtcg ccgtactcgc cgaagccgat gccaggcgaa
     8641 accgccacct tcgcttccgt cagcagtttc ttggagaatt cgatcgagcc catggcccgg
     8701 taagcctcgg ggatgcgtgc ccacacgaac atggtggcct tgggcttttc caccagccag
     8761 ccggcgccga acaggccgtt gcacaatacg tcgcgccggt tctggtaggt ctgccggatt
     8821 tcctcgaggc agtcttgcgg cccttcgagc gcgaggatgg ccgcgatctg gatcggcgtg
     8881 aacatgccgt agtcaaaata tgacttgagc cgggacagcg ccgcgaccag tttctggttg
     8941 ccgcacatga accctatgcg ccaaccaggc atgttgtagc ttttggagag cgtgaagaat
     9001 tccaccgcga cgtccttggc accgggcact tgcagaatag atggtgcgcg gtagccgtcg
     9061 aacacgatgt cggcgtaggc cagatcgtgc accacccaga tgccgtgctc cttcgcgatg
     9121 gcaatcactt tctcgaagaa atcgagttcg acgcattgcg tcgtggggtt ggccgggaaa
     9181 ttcagcacca gcatcttggg cttgggccag gcgttcttga tggatttttc cagctcggca
     9241 aaaaagtcga cgcccggcac cagcgggaca tgccggatat cggcgcccgc tatcacgaaa
     9301 ccgtaggcat ggatcgggta gctcgggttc ggcaccagca ccgagtcgcc cttgtccacg
     9361 gtcgccagtg ccagatgcga caggccttcc ttggagccga tggtcacgat cgcctcgctg
     9421 tccatgtcga gatcgacgtc gtaacggcgc ttgtaccatt ggcagatcgc gcgccgcagg
     9481 cgcgggatgc cgcgcgagac cgaataacga tgcgtgtctt cgcgttgggc cgcctccacg
     9541 agcttgtcca cgatgtgttt gggcgtggcg ccatcgggat tgcccatccc gaagtcgatg
     9601 atgtcttcgc cgcgccggcg cgcctgcgcc ttgagctcgt tgacgatgtt gaagacgtaa
     9661 ggcggcagcc gctttatgcg gggaaattcg tcgttttgtg acatgcgttt cgtcgggctg
     9721 ttatagtgtc gagcgactgc caagatattg aatacgcgtg cattataggg ctttcgtcat
     9781 agcgatggaa caaccggcca cggacagagc ttcagctttg cgatgaaaat tcaccttgaa
     9841 tcgggtactg ggcagaacct gatccgcgcc tacgcacccg gcagcatcac catcaacgat
     9901 gtggcttata ctcgcagcct gatcgtgacg cctgagcaga taatagactg gccaccagcc
     9961 agcttcgccg agatcttggc agtgcatttc gatgtgcttg ctgcgatgcg gccggaggtc
    10021 gtgatcctgg ggacaggcgc gaaattgcag ttcccatccc cagtcctcac ccgggcgctg
    10081 gtggatgaga atattggact tgaagtgatg gataccggcg cggcctgccg gacctacaac
    10141 atactcatga gcgatggccg gcgtgtcgct gcggccctgc tgatgattga gaacctaaaa
    10201 cagggaatcg actgagtagc cggctttttc cagaatcacg cgcagccgct tcagcgcttc
    10261 gacctggatc tggcgcacgc gctcacgggt aacaccgata tcggcaccga cttcttccag
    10321 cgtagagatc tcgcggtgat tcagcccgaa gcgacgctcg accacctcgc gctgcttgtc
    10381 attgagctcg ttcagccatt gctggatcaa ctgctgcaaa tcgtcgcgct gcaacacctt
    10441 ttccggatcc ggtgtgcgtt catcggctat ggcgtccagc aatgagcggt ccgggtcgtc
    10501 atccagtggc gcgtccacgg aagcgacgcg ctcgttgaga cccagcatac ccttcacgtc
    10561 ctccaccggc ttatcgagca gattggcgac ttcctccggg gacggctcgt ggtccagttt
    10621 ctgcgccaga taacgtgcgg cgcgctgata gatgttgatt tccttgagca cgtgcaccgg
    10681 caggcggata gtgcgcgtct ggttcatgat gccgcgctcg atggtttgcc gtatccacca
    10741 ggtcgcgtag gtggagaaac ggaatccgcg ctcggggtcg aatttctcca ccgcgcgaat
    10801 aaggccgagg ttgccttcct cgataaggtc gagcaatgaa aggccgcgat tcatatagcg
    10861 ccgcgcgatc ttgaccacca ggcgcaggtt gctttcgatc atgcgcttgc gcgccttgcc
    10921 gtcgcccttc tgcgccttgc gtgcaaaaaa tacctcttcc tcggcggaca ggagcggcga
    10981 gaagccgatt tccttcagat aaatgcgggt tgcgtcggcg tgtgaataat aggcgtcgcg
    11041 tttgtcgctt tcgctggcct cggcctcagg ttcgtcctct ttgctggcaa cttcggcggc
    11101 aggttccgca gtttcggttt cttcgtcctc ggagtcctcg gcggcaacga gggtttcatc
    11161 ctccatctct tcggactcgg gagatgcctt ggtcggctcg ggcttggcct tggattttcc
    11221 cttgcctttt ttgacgggcg ggtgactcgg tttgcgtggt ggcatggtta ctttctggga
    11281 agataatcct gcggatccac cggagcgccg cgatatcgca cttcaaaatg cagcttggtt
    11341 ctgtcggtgc cgctgctgcc catgtcggcg attttctgac cgcgttttat cacattccct
    11401 tccttgacgt aaatcttgtc gcagtgcgca taagcgctca gaaaatcggc gttatgtttg
    11461 acgatgataa gctgaccata accgcgaagt ccacttcctt gatacacgac ttgccctgct
    11521 tccgccgcga ggatcggttg gcccttcttg ccggcaatgt cgattccttt attcggtccg
    11581 ttggcggaat agcgctccag caattcgccc tgcgccggcc acgcccagga taactgcttc
    11641 gccgccgcga cgggcgcgtg ccgcaccggc gcggttggct ctggtttatc cggcgcctgt
    11701 tttttgtgaa tctccggctt ggaggaagcg ctgggttccg gacgacgtgc cttcggcact
    11761 cccgttggcg ggtagagtcg cagtctctgc ccgggcttga tgagataggg aggaggaatc
    11821 tggttccaac gggcgacatc ctgataatcg cgtccggatt cccaggcaat gctgtagagc
    11881 gtgtcgcccg gctgcaccac gcggtatccg gcctccggct gccgcaattc cctgccctgt
    11941 tcgctgaccg gagccaacgg aaagctgcca caggcgctta tgccgatcgc gagcataatg
    12001 atgccgcaga ttttcaggca ggagttcatc cgttccatcc cgaccgggga gcttgcgcct
    12061 tcagagagaa tctctcacca gcgggacgaa gttcaccagt tcgagtcgtt cctggatcag
    12121 gcccttgtcc gtatgttgca ccagcagcag ttcttgcatc gtcggcccgc caaccgggat
    12181 gatcaaccgg ccgccgggcg ccagctgctc gagcaaggcc gggggcactt ccgccggagc
    12241 ggctgtgacg atgatcccgt catagggtgc atgttccttc cagccccaac tgccgtctga
    12301 aagcttgatg tggacgttgc ggcaaccgat ctcgcgcagg cgtttgcgcg ccagcttcat
    12361 taacggttca agacgttcga cggagtagat ctcatccacc aggccggcca gcaccgccgt
    12421 ctggtagccg gagccggtac cgacttccag cacctttttc agacgcccgc cggcgagcag
    12481 cgtctgggtc atgagcgcca ctatataagg ttgagaaatg gtctggccgt gaccgatggg
    12541 cagggccgtg tcttcatagg cgcggctggc cagggcctca tcgacgaata catggcgcgg
    12601 cacattgcgg atggcctcca gaacgcgctc gtcgcgaata ccctcctcgc gcaatcgctg
    12661 gataagccgg tcgcgggtgc gccgcgaggt catgccgatg ccgtggacgt ccattctaac
    12721 cggtgttcgt tttctatgtt gatctcgcta gatttttctg agccaggcag aaacctgatc
    12781 cagcgcagta taacgggtca aatccacatg caaaggggtg atggaaacga actgattccg
    12841 gatggcatga aaatccgtcc ccggtccggc gtcggattcc gcgcccggcg cccccaccca
    12901 gtaaaccgga ttgccatgcg ggtcgctggt gcgaatcacc ggctctgcct tgtgacgatg
    12961 tccgaggcgc gtggcctcga aaccggccag ctgttccagt ggcacatccg gtacgttcac
    13021 gttcaatatg gtatccgccg gcaatgactc gcgttgcagc ttctccagca gacgcagtgt
    13081 gacatgcgct gcggtttcaa aatgcgctcc gacttttcca gccagcgata cggcgatcgc
    13141 cggaaggcca aggaaacgcc cttccatcgc cgccgccacc gtgccggaat agatcacgtc
    13201 gtcgccaaga ttcgcgccgc gattgatgcc ggcgatgacg atatcaggct cccgttccag
    13261 cagcccggtg atggcgagat gcacgcaatc ggtcggcgtg ccgtcaacga aataaaaacc
    13321 gttaccggcc ttctgcacgc gcaaggggtg agtcaatgta agcgagttac tggcgccgct
    13381 gcggtcgcgt tccggcgcta ccacgtccac ttgcgccacc tgctcgagcg ccttggccag
    13441 gcaggcaagg ccgggagcga ggtaaccgtc gtcattgctg aggagaattc gcattagcgc
    13501 tacttatcgt tttgtatgtg tgttgaaaac atctccgcgt accagcctga ggtggatagc
    13561 tcacctcccc gtagcgcgcg ccgagtacca cagccccacc gggagctttt aagcgagacc
    13621 gtgttcgagc ccaaggcgcg tttcccagcg cccggcgagt tggtcgagcg cccggtgggg
    13681 cgaggaacgg aggaagggtt cgcgcgctcc ggggtgtgtt tcttttggtt acttttcttt
    13741 gcacaagcaa agaaaagtaa cctgccgtgg gtcagccacc cacaagtaaa tacgcggccg
    13801 caggccgctc gacaaaacat tgatgcaaga aatctcggct tggcgaaatg ctcttgaggg
    13861 aacactgctg aaataaacac acgatcttgg tcggggcgag aggatttgaa cctccgacca
    13921 ccaccccccc acggtggtgc gctaccaggc tgcgctacgc cccgtttgtg tatttgcgtg
    13981 gaagggtccc gcatgatgta ccgcagagtt gcggtcgcca tcataccgga gaatcggctt
    14041 gaaacaagaa taatttagag tttcttcagc atgtccagga catcttccag ctcgttgatg
    14101 agcgtcttga tggcctgctg acggttggca tccggcggcg cgggattgtc cagttcgagt
    14161 ttgttgcgtg caccgctgat ggtgaaccct tcaacgtaaa gaagactgcg aatctgccgg
    14221 atcaggatca cgtcatgccg ctgatagtag cgacggttgc cgcgacgctt gaccggtttg
    14281 agctggggaa attcctgctc ccaataccgc agcacatgcg gcttgaccgc gcacaggtcg
    14341 ctgacttcac cgatggtgaa gtagcgcttg ccgggtatgg taggaagctc gtgattctcg
    14401 ccggggtcaa gcatttagtt ccgcctgctg tttgaacttt tcacggttcg cactggcgtt
    14461 ctgctcgact cgttccttga gtttatggct ggcgcgaaag gtaaccacgc gccgggcggt
    14521 aatcggaatc tcctcgcccg ttttcgggtt ccggccggga cgggagtttt tctgccgcag
    14581 ggagaaattg ccgaaaccgg agagttttac ctgctcccct tcggataagg ccccccgtat
    14641 cctttcaaag aaaatctcga caaattcctt tgcctcccgc ttgttcagac ccagctcgtt
    14701 aaacaggttt tccgcaagat ccgctttggt caatgccata ggtcgatacc ttcttttcct
    14761 tattccagag gtactttgta cattattgac ggggctgagc tcccaacccg gacttcaggg
    14821 cggacattac ctgtgtcatc acccgctcca cgtcatcctc attaagagtg cgcgaagagt
    14881 cctgcaaggt caagcccaaa gccaaacttt ttcttccgga atcaatgcct ttcccgcgat
    14941 attcgtcaaa tagctcaagg ttgaccagca accccccggc cgcttgctgc acgcaatcca
    15001 gcacagcctg ggctggcgtg gagtctttta caaggatagc aagatcgcgc cggatggaag
    15061 gaaatctcga aaattcacgg aaattaggga ttttcccgct ccgcagcgca cccactttaa
    15121 tctcgaacag taccaccgga cggtccaatc ccaatcggga ttgcgtggcg ggatgtaata
    15181 ccccaaccag gccaatctgg ttgtcatcga gccggatttc ggcggtttgt ccagggtgca
    15241 gggccgggtg ctgccccggc tgaaaacgga caccctgccc gatgccagcc aaaaccagca
    15301 aggcttcgac atcggccttc acgtcatgaa agtcgacggc gcgtcgcggt agcccccatt
    15361 gctctgccat ggcttcgcca caggcggcac cggccagcat gggttcctga ctgaaatcct
    15421 gtccttgcgg caggaaggtt cgcccgatct cgaagaagcg cgcacgggtc tgctggcggt
    15481 tctggttgta caagatggtc tgcaacagac cgggccagag cgaggtccgc atcaccgcca
    15541 tttccgcact gataggatta gccagcctgg ctggacttaa ctgcgggtcg atcaaggtct
    15601 ggattccggg atcgacaaaa ctgtaggtga cgacctcctg gtaaccgcgg tcaatcaagc
    15661 ctgcacgcag acgcgattcg ccaatatccg attccggcac cgggctggcg gccatgtcca
    15721 tgcgcggacg ccgactgggg agcctgtcat aaccgtgaat gcgcgcgatc tcttcgatca
    15781 ggtccacttc acggctgata tcgaaacgat gggacggcgg cgtgatcgcc cacttgttcc
    15841 ctgccttcct aagtttcatg ccgaggcgtt tcagaatgtc ttcgacctct ttcacaggta
    15901 tatccaggcc cagaagtcgg ctgacgcgct ccaggcgcag agtaaccggg gagacgcgcg
    15961 gaagatgacg cttcacggtc tgctcgacca agggtcccgg cctgccgccg accacagaca
    16021 gtaacagcgc ggtcgcccgc tccatggtca accgctgcaa ggcggggttc acaccgcgct
    16081 cgaaacggaa ggatgattct gtttgcaagc cgtgctcgcg tgagcgaatc gaaatggttt
    16141 ccggacggaa ccaggcgctc tccaggaata tgtcgcgcgt agcgtcgctc acggcggaat
    16201 ccatcccgcc catgatgccg gccagagcca acggttgcgc gtcatcggcg atcatcaggc
    16261 tgcccggctt cggtgtcagg cgattgccgt ccagcagaac cagcgattca ttcttcgcgg
    16321 cgtggcgcac atggatggaa ccgcggattt tcgaaagatc gaaggcatgc atgggctggc
    16381 ccagctcgat catcacgtaa ttggtgatgt ccaccaccgg atgaatgctg cgaataccgg
    16441 agcggcgaag tttttctttc atccatatcg gcgtgacggc gtgtggattt atcccggtca
    16501 ctacgcgccc gatgtaatgg ggacaatcct gctgtgcatc gagtttgacg gcgacgcgtt
    16561 gccggtgctt cggtttcacc ggcttgatgg cgggggattt cattttcacg ctggtgatgg
    16621 ccgcgagctc gcgcgccaga ccggccacgc tcaggcagtc gccgcggttc ggcgtcagat
    16681 cgacttccag cgaaacatca tccaggcaga gatattcggt taacgatgtg ccaggttttg
    16741 cgtcagcatc caaggccaat aagccttcgg cggattctgc cagacccaat tcctgcgctg
    16801 aacagagcat gccggaggaa gccgcgccgc ggatttccgt acgtttgatc aatatgccgt
    16861 tcggcagctt tgcgccctcc agtgccacgg gggctcgcat gccaaccgtg gcattcgccg
    16921 cgccgcaaac gatgtccagt attttgtctt tgccgatatc gacgcggcaa agcctgagtt
    16981 tatcggcgga tggatgcggc gcgatcgaaa tgattttgcc aacaatgacc ttgtccaatt
    17041 tcggcgccac gggttccacg gcgccaactt ccagcccggc catggtcaac cggtgcgcca
    17101 gcgcccgggt gtcgagcctg ggcgagaccc attcacgcag ccattgttcg ctaactttca
    17161 taatatgttt aaccgccaag gcgccaaggc gccaagaaaa acctggctga tcattttcaa
    17221 aatcttggcg ttcttggcgt cttggcggtt caatttattt acctgaactg ttgcaagaaa
    17281 cgtaaatcgt tttcgtagaa caaccgcaga tcgttcacgc cgtagcgcag catggccagg
    17341 cgttccacgc ccagaccaaa ggcaaaaccg gtccagcgct cgctgtcgat gccgacgtgc
    17401 cggaacacct ccggatgcac catgccgcaa ccgagcacct cgagccagcc ggtgttcttg
    17461 cagacgcggc aacccttggc cccgcagata acgcaactga tgtccacttc cgcggacggt
    17521 tcggtgaacg
//
LOCUS       DBNGZE010000020         3088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_419389, whole genome shotgun sequence.
ACCESSION   DBNGZE010000020 DBNGZE010000000
VERSION     DBNGZE010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_419389"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="AC16FV_00560"
     CDS             <1..860
                     /locus_tag="AC16FV_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR2848585.1"
                     /translation="GHDVVILTRHAARQRAILVLPTVRLAEGDVYDAEFLRKQFEGRD
                     SVINLVGILNEAGRSGKEFTRTHAELPEKIVLACRETGVQRLLHMSALHATRMAPSQY
                     LRTKALGEEAVHRADGQDFHVTSFRPSVIFGPGDGFLNRFAGLLRLAPGVFPLACPGA
                     RFQPVYVEDVVRAFAEALDNHKTFGQRYDLCGPRVYTLREIVEYVAKLIGKRVCVIGL
                     PDSLSYLQAATLEFFPGKPFSLDNYRSLQIDSVCEKGFPGVFGITPTSLEEIAPGYLK
                     KWNGQDSQD"
     gene            complement(863..1906)
                     /locus_tag="AC16FV_00565"
     CDS             complement(863..1906)
                     /locus_tag="AC16FV_00565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR2848586.1"
                     /translation="MKYCHRLLALLLLIVVIGGCASPGYYLHAVSGQVELLNKRRPLE
                     EVLNDPATTPEIRQQLELVRRLRDFASRELGLPDNRSYRTYADLERPFVVWNVFATPE
                     LSLEPKQWCFVFAGCVSYRGYFARDRAEQFAADLKQDGYDVYVGGVPAYSTLGWFDDP
                     LLNTFIHRSEADLAGLLFHELAHQIIYVGGDSAFNESFATVVELEGVKRWFERHGTPQ
                     QAEAYRRKIQRREQFTTLVLKHKASLAGIYATNSSDAEKRTAKTRVFEELRSDYAALK
                     IKWDGNADYDRWFAQDLNNSHLVAIGMYSQYVSAFQKLLKQQDTNLTAFYRAVKEMAS
                     QPKNERDITLQKF"
     gene            1953..>3088
                     /locus_tag="AC16FV_00570"
     CDS             1953..>3088
                     /locus_tag="AC16FV_00570"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HIR2848587.1"
                     /translation="MKTYLVGGAVRDKLLGLPVQDRDYVVVGSTPEEMVAKGFKPVGA
                     DFPVFLHPETKEEYALARTERKSGHGYKGFKVYAAPDVTLEDDLRRRDLTINAMAEDE
                     QGQLIDPFKGAEDLRNGVLRHVSPAFAEDPVRILRVARFAARYGRWDFHVAHSTNKLM
                     REMVESGEVDHLVAERVWTELERALGEDRPSRFFETLRGCAALARLFPEIDALFGVPQ
                     PEKHHPEVDTGVHIMLVLDAAAKLSPDTRVRFAALMHDLGKGNTPKVEWPQHIGHEER
                     GVELVKNFCQRFRVPNEHRDLAVLTARYHAHCHKIAELRAATVVDTLEALDGFRRPER
                     IGQFVLACEADFRGRTGSEDKPYPQAELFRRTFEAARAVDTAALA"
BASE COUNT          596 a          929 c          959 g          604 t
ORIGIN      
        1 ctgggcacga cgtcgtcatc ctcacgcgtc acgcggcgcg gcagcgcgct attctggtgc
       61 tgccaacggt gcggctggcg gagggcgacg tgtacgacgc ggaatttctg cgaaaacaat
      121 ttgaaggccg ggatagcgtc atcaacctgg tcggcatcct gaacgaagcg ggccgctcgg
      181 gaaaagaatt cacacggacg cacgccgaac tgccggaaaa aatcgtgctg gcttgccgcg
      241 aaaccggcgt gcagcgcctg ttgcacatga gcgcgctgca tgccacgcgc atggccccaa
      301 gccaatatct gcgcaccaag gcactgggcg aggaggcggt gcaccgcgcc gatggccagg
      361 actttcacgt caccagcttt cgcccctcgg tcatcttcgg tcccggcgac ggtttcctga
      421 accgcttcgc cggcctgctg cgcctggccc cgggcgtgtt cccgctcgcc tgtcccggcg
      481 cgcgtttcca gccggtgtac gtcgaggacg tggtacgcgc cttcgcggaa gcgctcgaca
      541 atcacaagac cttcgggcag cgctacgacc tctgcggccc ccgggtttat acgttacgcg
      601 agattgtgga atatgtggcg aaactgatcg gtaaacgtgt gtgcgtcatc ggcctgcccg
      661 atagcctgtc ttatctgcaa gcggcgacac tggaattttt tccgggcaaa ccgttctcgc
      721 tcgacaatta tcgctcgctg caaatcgata gcgtgtgcga gaagggcttt cccggagtat
      781 tcggcatcac gccgacaagc ctggaagaaa ttgcgcctgg ttatcttaaa aaatggaatg
      841 gacaagattc acaggattaa aatcaaaatt tctgtaaagt tatgtcgcgc tcatttttcg
      901 gctggctcgc catttccttt actgcgcgat agaaagccgt aaggttagtg tcctgctgct
      961 tcagcagttt ctgaaatgcc gaaacgtact ggctatacat gccaatcgca acgagatggg
     1021 aattgttgag gtcctgagca aaccacctgt catagtcagc attaccatcc cactttattt
     1081 tcaatgctgc gtaatcactc cgtagttcct cgaatacccg tgttttggca gtgcgttttt
     1141 ccgcgtcgct tgaattcgtg gcgtaaatcc cggccagact tgccttgtgt tttagcacca
     1201 gcgtagtgaa ttgttcgcgc cgctggattt tccgccgata ggcctcggcc tgttgtggcg
     1261 tgccgtggcg ctcgaaccag cgcttcacgc cttccagttc cacgacggtg gcgaaggatt
     1321 cgttgaaggc gctgtcgccg ccgacgtaga taatctgatg cgccaactcg tggaacagca
     1381 agccggcgag atcggcctcg ctgcggtgga tgaaggtgtt gagcagcggg tcgtcgaacc
     1441 agccgagcgt ggagtaggcc ggaacgccgc cgacatagac atcataaccg tcctgtttga
     1501 gatcagcggc aaactgctcc gccctgtcgc gcgcaaagta gccgcgatag gacacgcagc
     1561 cggcaaacac gaagcaccat tgcttcggct ccagcgacaa ttccggcgtg gcgaacacgt
     1621 tccacaccac aaacggccgt tccagatcgg cataagtccg gtagctgcga ttgtccggca
     1681 ggcccagctc gcggctggcg aagtcgcgca ggcgtcgcac cagctcaagt tgttgccgga
     1741 tttccggggt cgtggccggg tcgttcagca cctcttccag cggccggcgc ttgttgagca
     1801 gttcgacctg ccctgaaacc gcgtgcaggt aataacccgg actggcgcag ccgccgatca
     1861 cgacgatcaa gagcaacagg gcgagcaggc gatggcaata tttcatgaat cccggctttg
     1921 cgtcagtagc agccacacat tagcatggca gtatgaaaac ttatctcgtc ggcggcgcgg
     1981 tgcgcgacaa gctcttgggc ctgccggtcc aggaccgcga ttacgtcgtg gtcggcagca
     2041 ccccggagga gatggtggcc aagggcttca agcccgtggg cgccgatttc cccgtgttcc
     2101 tgcatccgga aaccaaggag gaatatgcgc tcgcgcgtac cgagcgcaaa tcgggccacg
     2161 gctacaaggg cttcaaggtg tacgccgcgc cggacgtgac gctggaagac gacctgcgcc
     2221 gccgcgacct caccatcaac gccatggccg aagacgagca gggccagctg atcgatccct
     2281 tcaagggcgc ggaggatttg cgcaatggcg tgctgcgcca tgtctcgccg gcattcgccg
     2341 aggacccggt gcgcatcctg cgcgtggcgc gtttcgccgc gcgctacgga agatgggatt
     2401 tccatgtggc gcattccacc aacaaactca tgcgcgagat ggtcgaatcc ggcgaggtgg
     2461 atcatctggt ggcggagcgt gtgtggacgg agctggagcg cgcactggga gaagacaggc
     2521 cctcgcgctt tttcgagacg ttgcgcggtt gcgcggcgct ggcgcggctg tttccggaga
     2581 tcgatgccct cttcggcgtg ccgcaaccgg agaagcatca cccggaagta gacacgggcg
     2641 tgcatattat gctggtgcta gatgcagcgg cgaagctctc gcccgacacg cgcgtgcgct
     2701 tcgccgcgct tatgcatgat ctcggcaagg gcaacacgcc gaaagtggag tggccacagc
     2761 atatcgggca cgaggagcgc ggcgtggagc tggtgaaaaa tttctgccag cgttttcgcg
     2821 tgccgaacga gcatcgcgac ctggccgtgc tcacggcacg ttaccacgcc cattgccaca
     2881 agatcgctga actccgggcg gccacggtgg tcgatacgct ggaggcgttg gatggcttcc
     2941 gccgtccgga gcggatcggc cagttcgtgc tggcctgcga ggcggatttc cgcggccgca
     3001 ccggcagcga agacaagccc tatcctcagg cggagctgtt tcgccgcacc ttcgaggccg
     3061 cccgcgcggt cgataccgcc gcgctggc
//
LOCUS       DBNGZE010000021         4201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1461620, whole genome shotgun sequence.
ACCESSION   DBNGZE010000021 DBNGZE010000000
VERSION     DBNGZE010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1461620"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="AC16FV_00575"
     CDS             complement(<1..163)
                     /locus_tag="AC16FV_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2848588.1"
                     /translation="MKPYTPAPDLLTSRRILITGAGDGIGRAVALACARHGASVILLG
                     RTAKKLEKIY"
     gene            325..540
                     /locus_tag="AC16FV_00580"
     CDS             325..540
                     /locus_tag="AC16FV_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848589.1"
                     /translation="MKKFFIGLALGLIVAFPLGINFGKDKPLLSNPFAAKPDITEKVK
                     ERTGEMLKETKEAIHDATKPMKDKLEK"
     gene            complement(558..1529)
                     /locus_tag="AC16FV_00585"
     CDS             complement(558..1529)
                     /locus_tag="AC16FV_00585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR2848590.1"
                     /translation="MKPEQAKKTADPLPLLLLVEDSQTTTALLSKYLGHGYRLLHAKD
                     GALAWDMLEQNPEIALVITDIHMPNMTGHQLLVKIRKSEDPRYKNLPVIVMTTTEDNV
                     DRNLAFLNGANDFVTKPIDEMEMVARVNVHYRLAKTIRELEASRYALSEQATTDALTR
                     LKNRRAFFENGAKAVVMARRYVSDLSVILLDIDHFKKINDTHGHSAGDEALVMVANIL
                     LELSRTEDTVARIGGEEFALLLPDTNRLGTAVLAERIRSAIEREQFIVGDKIVPMTAS
                     IGIASFGVDPAESIDQLLGVADNRLYLAKNAGRNRICVNDEGKTTFA"
     gene            complement(1608..2831)
                     /locus_tag="AC16FV_00590"
     CDS             complement(1608..2831)
                     /locus_tag="AC16FV_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR2848591.1"
                     /translation="MSNAVTLIIVAIVILLAGGLGWFVAQLRTQKRLAELATTLELER
                     KAAQEKLAGLEQNFIALSQRALKENNAAFLQLAQESLKQFHVQAKGDLELKEKAVENL
                     IKPVREALEKTEQQIRLMENERKEAYGSLSKHLETMAQTQQLLQGETRNLVQALRRPE
                     VRGQWGELTLKRLAELAGMVEHCDFYEQEHTNTEEGRMRPDMIVRMPGGREIVVDVKT
                     PLDAYLSAVEATDDETRRKHLEHHARKVRERIRELSSKAYWTQFKSAPDFVILFIPGE
                     QFLTAALDMDRELIEEALKQKVILTTPTSFVALLRAVAYGWRQESLAANAEKIRDVGE
                     ELYGRLMTFSEHLMKLGRSLNGTVADYNKTVGSFEAKLLPGARKFSEMGVGGADKALA
                     EPERIDKAVRDVQQG"
     gene            complement(2824..3489)
                     /locus_tag="AC16FV_00595"
     CDS             complement(2824..3489)
                     /locus_tag="AC16FV_00595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2848592.1"
                     /translation="MTKIRTVLFDLDGTLADTAPDLAHALNVLLVEEGKKALSYETIR
                     PEVSHGASALVTLGFGKKPGDPDFDRLRSRFLALYTENLCRHTRLFEGIPSLLQTLKK
                     QGLNWGIVTNKPAFLTDPLVAQLRPEPAPACVVSGDTVANRKPHPEPMLHACTAAGSR
                     PEQCLYVGDAERDILAGRHAGMKTLVALFGYINSHETPERWGADGMIRAPGDILDWLK
                     KDE"
     gene            complement(3482..4192)
                     /gene="ubiG"
                     /locus_tag="AC16FV_00600"
     CDS             complement(3482..4192)
                     /gene="ubiG"
                     /locus_tag="AC16FV_00600"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR2848593.1"
                     /translation="MTHTAQNVDPEEIAKFESLAARWWDPHSEFKPLHDINPLRLNYI
                     NDRVGLKDKTVLDIGCGGGILAESMAAHGATVTGIDLGDAPLAVAKLHLKESGQKVEY
                     RKISAEDMARERPESFDVVTCMEMLEHVPEPASTIEACAKLVKPGGQVFFSTINRNPK
                     SWLFAIVGAEYVLNLLPKGTHEYMKFIKPSELERWARHAGLTLRELIGMHYNPLTRQY
                     RLGRGVDVNYIAYITRGD"
BASE COUNT          697 a         1266 c         1353 g          885 t
ORIGIN      
        1 catagatctt ttccagtttt ttggcggtgc gtccaagcag gatcacgctg gccccgtggc
       61 gcgcgcaggc gagcgccacg gcgcgcccga tgccgtcgcc ggcgccggtg atgaggatgc
      121 ggcgcgaagt cagcagatcg ggggcggggg tgtagggttt catgtttgtt tcaggcggca
      181 ggaccagcgt gaattgtagc gcaacaggac tggccgcgca gtggccaagc cccgcgcgga
      241 tgtactatta tggcaagacg aacgcagcga agcggagtag ggctactgct ctcatgtgac
      301 gcggttcaag acagagagga cgtcatgaag aaattcttta ttggtttagc gctgggtctg
      361 atcgtcgcct ttccgctggg aatcaatttc ggcaaggaca agccgctgct cagcaatccg
      421 ttcgccgcca agccggacat taccgagaag gtcaaggagc gcaccggcga aatgctcaaa
      481 gagaccaaag aggcgattca cgacgccacc aagccgatga aagataaact ggaaaaataa
      541 tctcccgtcg ggcggcgtca ggcgaaagtg gtcttgccct cgtcgttgac gcaaatgcgg
      601 ttgcgcccgg cattcttggc gagatacaga cggttgtccg ccacccccag cagctgatcg
      661 atgctctccg cggggtccac cccgaaggag gcgatgccga tgctggcggt catcggcacg
      721 atcttgtcgc cgacgatgaa ttgctcgcgc tcgatggcgg agcggatgcg ttccgccagc
      781 actgccgtcc cgaggcggtt ggtgtccggc aacagcaatg cgaattcctc gccgccgatg
      841 cgcgccaccg tgtcctcggt gcgcgataat tccagcagaa tattcgccac catcaccagc
      901 gcctcgtcgc ccgccgagtg gccgtgagtg tcgttgattt tcttgaagtg gtcgatgtcg
      961 agcaggatca ccgacaggtc cgagacatag cgccgcgcca tcaccactgc cttggcgccg
     1021 ttctcgaaga aggcgcggcg gttcttgagt ctggtcaacg cgtccgtggt ggcctgttcc
     1081 gacaatgcat accggctcgc ctccagttcg cgaatggtct tggccagccg gtaatgcacg
     1141 ttcacgcgcg ccaccatttc catctcgtcg atcggcttgg tcacgaaatc attggcgccg
     1201 ttgagaaagg ccaggttgcg gtcgacgttg tcctcggtgg tggtcatgac gatgaccggc
     1261 aggtttttgt agcgggggtc ttcgctcttg cggatcttca ccagcaactg gtgcccggtc
     1321 atgttcggca tgtggatgtc ggtgatgacg agcgcgatct ccgggttttg ttcgagcatg
     1381 tcccaggcca gcgcgccgtc cttcgcgtgc agcaggcgat aaccgtggcc aagatatttg
     1441 gacagcaggg cggtggtcgt ctgggagtcc tccaccagca ggaggagggg cagcgggtcg
     1501 gcggtcttct tggcctgttc aggcttcacg ttgcgtcacg tcagcaggct caaaatgagc
     1561 cgggtctgct gaaaatatag cacgccgctg cccggccatt cggccggtca gccctgctga
     1621 acgtcgcgga ctgccttatc gattcgctca ggttccgcca gagccttgtc cgccccgccc
     1681 acgcccattt ccgagaactt gcgtgccccc ggcagcagct tggcctcgaa ggagccgacc
     1741 gtcttgttgt aatcggccac ggtgccgttc aggctgcggc cgagtttcat gaggtgttcg
     1801 ctgaaggtca tgagacggcc gtacagttcc tcgccaacgt cgcggatttt ctcggcgttg
     1861 gccgcgaggc tttcctggcg ccagccgtag gccacggcgc gcagcagggc gacgaagctg
     1921 gtcggagtgg tgagaatcac tttctgcttt aacgcctctt cgatcagttc gcggtccatg
     1981 tccagtgcgg cggtcaggaa ctgctcgccg ggaatgaaca ggatcacgaa atccggcgcg
     2041 ctcttgaact gtgtccagta ggccttgctc gacagctcgc ggatgcgctc gcgcaccttg
     2101 cgcgcgtgat gttccagatg cttgcggcgc gtctcgtcgt cggtcgcctc gaccgcgctc
     2161 aggtaggcgt cgagcggcgt cttcacgtcc accacgattt cgcgcccgcc gggcatgcgc
     2221 acgatcatgt cggggcgcat gcggccttcc tcggtattcg tatgttcctg ttcgtagaag
     2281 tcgcagtgct cgaccatgcc ggccagctcg gcgagtcgct tcagcgtcag ctcgccccac
     2341 tggccgcgca cctccggccg gcgcagcgcc tgcaccaggt tgcgcgtctc gccttgcagc
     2401 agttgctggg tctgggccat ggtttccaga tgcttggaca gcgagccgta ggcctccttg
     2461 cgctcgttct ccatcaggcg gatctgttgt tcggtttttt ccagtgcctc gcgcaccggc
     2521 ttgatcaggt tctcgaccgc cttttccttc agctccagat cgcccttggc ctgcacgtgg
     2581 aattgcttga gcgattcctg cgccagttgc aggaacgccg cgttgttttc cttgagcgcg
     2641 cgctgggaca gggcaatgaa gttctgttcg aggccggcaa gtttttcctg tgccgccttg
     2701 cgttccagtt ccagcgtggt ggcgagttcg gcgaggcgct tctgggtgcg cagttgcgcg
     2761 acgaaccagc ccagccctcc cgccagcagg ataacgatcg cgacaataat aagtgtgacg
     2821 gcgttactca tcctttttca gccaatcgag aatgtcgccc ggcgcgcgga tcatgccgtc
     2881 cgcgccccag cgttccgggg tttcgtggct gttaatgtac ccgaacagcg ccacgagcgt
     2941 cttcatgccg gcgtggcggc cggccaggat gtcgcgctcg gcgtcgccga catacaggca
     3001 ttgctccggg cggctgccgg ccgcggtgca ggcgtgtagc atcggttccg gatgcggttt
     3061 gcggttggcc acggtgtcgc cgctgaccac gcaggccggc gcgggctcag gccggagctg
     3121 cgccaccagc ggatcggtga ggaaggccgg cttgttggtg acgatgcccc agttgagccc
     3181 ctgtttcttg agcgtttgca gcaaggacgg gatgccctcg aacaggcgcg tgtggcggca
     3241 gagattttcg gtatacagcg cgaggaagcg gctccgcagg cggtcgaagt ccggatcgcc
     3301 tggtttcttg ccgaaaccga gtgtcaccaa tgccgatgcg ccgtgtgaca cctcggggcg
     3361 gatggtttcg taggacagag cttttttgcc ttcctcgacc agcagcacat tcagcgcatg
     3421 cgccaggtcc ggcgcggtgt cggccagcgt gccgtcgagg tcgaacagaa ccgtgcggat
     3481 cttagtcacc gcgcgtgatg taggcgatgt agttgacgtc caccccgcgt ccgaggcggt
     3541 actggcgcgt cagcgggttg taatgcatgc cgatcagctc gcgcagcgtc aggcccgcgt
     3601 gccgtgccca gcgttcgagt tcggacggct tgatgaattt catgtattcg tgcgtgccct
     3661 tcgggagcag attcaaaaca tattccgctc cgacaatcgc gaacagccac gacttcgggt
     3721 tgcggttgat ggtggagaag aacacctgac cgccgggctt gaccagcttg gcgcaggctt
     3781 caatcgtgga ggcgggctcg ggcacatgct ccagcatttc catgcaggtc acgacgtcga
     3841 aggattcggg acgctcgcgc gccatgtcct cggcgctgat cttgcggtat tcgaccttct
     3901 gcccggattc cttcagatgc agcttggcga ccgcgagcgg ggcgtcgccc agatcgatgc
     3961 ccgtgacggt ggcgccatgc gccgccatgc tctcagcgag aatgccgccg ccgcagccga
     4021 tgtcgagcac ggtcttgtcc ttcagcccga cgcgatcatt tatatagttg aggcgcagcg
     4081 ggttgatgtc gtgcagcggc ttgaactcgc tgtgcgggtc ccaccaacgc gccgccaggg
     4141 attcgaactt ggcgatttcc tcggggtcaa cgttttgggc agtatgggtc atataaattc
     4201 a
//
LOCUS       DBNGZE010000022         9388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_489319, whole genome shotgun sequence.
ACCESSION   DBNGZE010000022 DBNGZE010000000
VERSION     DBNGZE010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_489319"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="AC16FV_00605"
     CDS             complement(<1..971)
                     /locus_tag="AC16FV_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018140251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR2848594.1"
                     /translation="MTDFLSEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGSV
                     KGRKAARHATSDWMELEKQRGISVTSSVMQFPYQSHIINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDGAKGVEERTIKLMEVCRLRHTPIMTFINKLDRESRDPVELMDEVETVL
                     KIRCAPVTWPIGSGKRFKGVYHLAEDSVHFFSPQHGGKIQRGVVIRGLHNPELDDILG
                     EQAAELRAEIELVKGASHAFDPQQYLRGEQSPVFFGSAINNFGIRELLDDFVQHAPPP
                     LPRATLTRTVAPDEPALTGFVFKIQANMDPKHRDRIAFLRITSGKYV"
     gene            complement(1144..1350)
                     /locus_tag="AC16FV_00610"
     CDS             complement(1144..1350)
                     /locus_tag="AC16FV_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018137804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2848595.1"
                     /translation="MRTGTVKWFNDSKGFGFIKPQDGGEDVFVHHSVIQGSGFKSLAE
                     GQSVSFESQKGPKGMQATKVVVNA"
     gene            complement(1532..1789)
                     /locus_tag="AC16FV_00615"
     CDS             complement(1532..1789)
                     /locus_tag="AC16FV_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848596.1"
                     /translation="MLPVYPTPRDIGLKIPPYLRETRFNAGFEHALKGGHLTEVEYFR
                     RSFRLGFRAAKLYLREVRRHRGILDVPMRARFRLRALWRGV"
     gene            1888..2751
                     /locus_tag="AC16FV_00620"
     CDS             1888..2751
                     /locus_tag="AC16FV_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2848597.1"
                     /translation="MADSPYVFDATAENFHALVLENSDKGPVLVNYWSPRAGPCLMLM
                     PRLVRLATEFGGRFLLVMLNTDELGRLAREHGVNSLPTMKLFRRGKVVDTLHGAESET
                     ALREFISKHLARESGGKYASALQAYQRGDLDLAVTQAAEAALAEPDNPRLAIDLVKLL
                     MLQRRYSQADALLKSLPDEVRDHPEIRNLSAHVGFIRAAQDAPPVERLEHAIGNNPDD
                     LEARYQLGALKMAQDRYEEAMQQLLEIVRRDNAFRHQAGRDGLLAIFSLLGESDERVQ
                     RYRSLLNQALH"
     gene            2919..3446
                     /locus_tag="AC16FV_00625"
     CDS             2919..3446
                     /locus_tag="AC16FV_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2848598.1"
                     /translation="MSARSTCFFLLLFLLTPAMPAHSAGLDDADERLFKVQLAMAEKG
                     DTRAQYYLGEMHEQGLGTKQDVDEAFKWYTKAAERGDPMAKRKLAHRQEIISEIKNEQ
                     AVEKLKSSATADIPAKPADALSRSTPTAVAQSDQARQQERDRIKATERDKKRAMVRAM
                     ILERMRHPVGAPFGE"
     gene            complement(3468..4655)
                     /locus_tag="AC16FV_00630"
     CDS             complement(3468..4655)
                     /locus_tag="AC16FV_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR2848599.1"
                     /translation="MATIKTFSGLTGFYVLTSDGELGAYDPDGKFVVDAKLTKAFADY
                     NRRFLMTYLQSPEKGNFRLAIIHRTQEKRPWGSETLLLEKSIVRRLYPMTGTTELDLT
                     AFQKKNREKSIYRGMEIFLEYKDASTPDLPVYCPVLYDRDTTLDDYAANLDRPVTSAD
                     RETPVVEILNLLNGIPVGRRYTPEITILKEKIYQGIVKKGMRKAAEFTLIEDIRRNAV
                     VAKGEPTDDPYADVDKRSERQVTLDSKTGDQAGNHASDSAFIRGISSSLVGRPEPAEP
                     DILKAFTRFRNLGHEKLAVLAERCLVYKVPPGTQLLERGTNDSWNLYLVDGIVQLVAA
                     DGVSKNIEGGTSTASNPISCLKPRMYTVSAVTRVAFLWIDDKLVDEIVKSAAAPRRLG
                     GAA"
     gene            5178..6146
                     /locus_tag="AC16FV_00635"
     CDS             5178..6146
                     /locus_tag="AC16FV_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HIR2848600.1"
                     /translation="MSFIQQAVLAKKAALAEVVSEPMARLGNRCAEVWPNADALDRLL
                     REAIGSIPHCHLLYAWDLNGIEISSMVQASGADPSWRGRDLSQRPYLKNHLPFKGVML
                     SSVYMSQYVPRQCITALQAVSRDNQLLGFIAADFALDDLLHDSHLTAPDMHWQQFRGD
                     PAVRGTVFMQERVPSLLDQHIDEVHDLIHRLMSHHGVFHSKIHFSSGRCSLWLFNDPY
                     NYRLHRVDEIINPDFCLAYPVCPYPDNAKVSPDQIRHVLDEFKALRFADETIYLRSSS
                     LNIMNGLVGLTFSCDGSHYMPVEEFMEKDLSFWLGSLNIKPEAAAV"
     gene            6327..6551
                     /locus_tag="AC16FV_00640"
     CDS             6327..6551
                     /locus_tag="AC16FV_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848601.1"
                     /translation="MKTVNLQLITIGLENGQQGVFVGIPLITEENSDLDSQVEEIWFS
                     NIQEIPDDLSVKKLMHLIASQLCRCRASLQ"
     gene            complement(6624..7139)
                     /locus_tag="AC16FV_00645"
     CDS             complement(6624..7139)
                     /locus_tag="AC16FV_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR2848602.1"
                     /translation="MSDSKTVIYRGKIIDFFLERVTLPNGAMAELEIVRHPGGAAVVA
                     LDSENRVCLLRQYRHAAGGWLWELPAGKLDPGEPPLVTAQRELQEEAGLRAARWEPLG
                     KIISSPGVFTEVVHLFLARDLRGTPANAEAHEVFEVHWVPFAEALMQARAGEITDAKT
                     LAGLFRADGLC"
     gene            7293..8072
                     /locus_tag="AC16FV_00650"
     CDS             7293..8072
                     /locus_tag="AC16FV_00650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR2848603.1"
                     /translation="MRRPIFMSLFGATLAGMFLGSCATVPETGRSQLLIVSPAQETQL
                     GFQEFEKLKKSLPISKDAAANAQLQRVGSRIAAIAPLPNARWEFVLFDKPDTPNAFCL
                     PGGKVGVFSGILPITKDDAGLATVIGHEVAHAVARHGAERMSQDTVFQILEELALGRS
                     SQASRSVIQQAYGLGFALPHSRQQELEADHLGVLYMARAGYQPREALAFWKRFAEYKN
                     KNGGGKQVEFLSTHPLDEQRIRQIEGLIPQAEMEYRKPDVR"
     gene            8247..8756
                     /locus_tag="AC16FV_00655"
     CDS             8247..8756
                     /locus_tag="AC16FV_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848604.1"
                     /translation="MFHRRLYFIVPDEAQAAQIVTDLEAAGVGRRHIHAIGGKGVTLE
                     KLPAANERQQHDTVWRLQQILWLGNLGLFVLGAIGLIVSVIQDSLTGAVLAFAAMALT
                     LTAGALFAIRVPDTHLDEFRGVLAHHEVLLMVDVPKQRVAEIEELIYRRHPEATAGGT
                     GWTIEALQI"
     gene            8889..9113
                     /locus_tag="AC16FV_00660"
     CDS             8889..9113
                     /locus_tag="AC16FV_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848605.1"
                     /translation="MFNIVCRTDPISGKDLFNTAGLPYVTHRSDETSLTIYFESENNR
                     HIYLEMPVEHPTSELSVNLDNPTDEMIDEG"
     gene            complement(9177..>9388)
                     /locus_tag="AC16FV_00665"
     CDS             complement(9177..>9388)
                     /locus_tag="AC16FV_00665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2848606.1"
                     /translation="GMVLFLLDIARQNKARVALLEVRMSNTAAYRLYTKLGFDEVGLR
                     KSYYPARHGREDAIILARDLTVGNY"
BASE COUNT         2042 a         2744 c         2685 g         1917 t
ORIGIN      
        1 tttacatatt tgccggaggt gatgcgcaaa aaggcgatgc gatcgcgatg cttggggtcc
       61 atgttagcct ggattttgaa cacgaaaccg gtcagcgccg gctcgtccgg cgcgaccgtg
      121 cgcgtcaggg tcgcgcgcgg gagtggcggc ggcgcgtgct gtacgaagtc gtcgagcagc
      181 tcgcgtatgc cgaaattgtt gatggccgag ccgaaaaaaa ctggcgactg ctcgccgcgc
      241 agatactgct gcggatcgaa tgcatgactg gcacccttga ccagctcgat ctccgcgcgc
      301 agttccgcgg cctgctcgcc gagaatatcg tccagctccg gattatgcag accgcggatc
      361 accacgcccc gctgaatctt gccgccgtgt tgcgggctga agaaatgcac gctgtcctcg
      421 gccaggtgat acacgccctt gaagcgcttg ccgctgccaa tgggccaggt caccggcgcg
      481 caacggatct tgagcacggt ttcgacctcg tccatgagct cgaccgggtc gcgcgactcg
      541 cgatcgagct tgttgataaa ggtcatgatc ggcgtgtgcc gcaggcggca aacttccatc
      601 agcttgatgg tgcgttcttc gacgcccttg gccccgtcga tcaccatcag cgccgagtcc
      661 accgccgtca gggtgcgata ggtgtcctcg gaaaaatcct cgtggccggg cgtgtcgagc
      721 agattgatga tgtggctctg atacgggaat tgcatcaccg aggaggtcac ggagataccg
      781 cgttgtttct ccagctccat ccagtcggat gtggcgtgcc gcgccgcctt gcgccccttg
      841 accgaaccgg cgagctggat cgcgccgccg aacagcaaca gcttttcagt gagcgtggtc
      901 ttgcccgcgt cgggatggga gatgatggcg aaggtgcggc gccgtgcgat ctcggacaga
      961 aaatcggtca tgcttgatgc aataaaatca attgggcgat tctatacaat ttcgccggga
     1021 gcgaaattgg acagccgaag gctggcccga gtcgctcgcg gcgagggtga gccccaagga
     1081 cggggcgaac aaaaaagaaa ccccgccgta gcggggtttc caaacagacg gcttatgtca
     1141 gtcttaggcg ttgacgacga ccttggtcgc ctgcatgcct ttcggaccct tctgggactc
     1201 gaagctcacg ctctgcccct cagccagtga cttgaaaccg ctgccctgga tgacggagtg
     1261 atgcacaaac acatcttcac cgccgtcctg cggcttaatg aagccaaagc cctttgaatc
     1321 gttgaaccat tttactgtac cagtacgcat taataacctc gaaatgaaag taagtttttt
     1381 tgttgcagag cgtgaaagga atttgcggga gcgatcttgt agcggatcca ccgaaatact
     1441 gacaacaaac tgcagcattg acactatagc cgatcacccg gagaaatgcc acccggggct
     1501 tggaagttcc gccaccggcc gtggtcgggc gtcaaacgcc gcgccacagg gcacgcagcc
     1561 ggaaccttgc ccgcatgggg acatccagta tcccgcggtg ccggcgaacc tcgcgcagat
     1621 agagcttggc ggcccggaat ccgagccgga atgagcgccg gaaatactcg acctccgtca
     1681 ggtgaccacc cttcaaggca tgttcgaaac cggcattgaa gcgggtttcc cgcaaatagg
     1741 gtggaatttt cagacctatg tcacgcggcg tcggatagac aggcagcatc gtaacctcca
     1801 cggcaagggg cctaattcca atatagaccc ttccggggcc gtatgtttcc ttgcgctccg
     1861 ggcaggtctt ttaatatcgt gcaaccgatg gccgattccc cttacgtttt cgacgccacg
     1921 gcggagaatt tccacgcgct ggtgctggaa aattccgaca aagggccggt gctggtgaac
     1981 tactggtcgc cgcgcgccgg gccctgcctg atgctgatgc cgcgtctcgt gcgtctcgcc
     2041 accgagttcg gcggacgctt tctgctggtg atgctcaaca ccgacgagct cgggcgtctg
     2101 gcgcgcgaac acggtgtcaa cagccttccc acgatgaagt tgttccggcg cggcaaggtg
     2161 gtggatacgt tgcacggcgc cgagtcggaa acggcgttgc gcgagttcat ttcgaaacat
     2221 ctggcgcgcg aatccggcgg caaatacgcc tccgcgctgc aggcttatca gcgcggcgac
     2281 ctggacctgg ccgtgacgca ggcggcggaa gcggcgctgg ccgaaccgga taatccgcgc
     2341 ctcgccatcg atctggtgaa attgctcatg ctgcaacggc gctactccca agctgacgcg
     2401 cttctgaaat ccctgccgga tgaagtccgc gatcacccgg aaatacgcaa cctgtccgca
     2461 cacgtgggtt tcatacgcgc ggcacaggat gccccgcccg tcgaacggct ggagcatgcc
     2521 atcggcaaca accccgacga tctcgaggcg cgttatcaat tgggcgcgct gaaaatggcg
     2581 caggatcgat atgaagaagc catgcaacag ttgctggaga tcgtccggcg cgacaatgcc
     2641 tttcgccatc aggccgggcg tgacggcctg ctcgcaatct tcagcctgct gggcgagagc
     2701 gacgaacgtg tccaacgcta ccggtccctg ctgaaccagg ccttgcactg aattggcgcg
     2761 aaggatagtg aggggagttc cccttgaaat accggtatta atccttgtct caacgggcgg
     2821 aatgagccgc ttgtcggtcg caaggcatat cccgaggcca tcatcgtcta tatacaggtg
     2881 atagacggca gcaaggagtc gttctcgggg ggggttccat gtcagccagg tccacatgtt
     2941 ttttcctgct tctttttctg ctcacgccgg ccatgccggc gcacagcgcc ggtctcgacg
     3001 atgccgatga gcgcctgttc aaggtgcagc tggcgatggc cgagaaaggc gacacccggg
     3061 cgcaatatta tctcggcgag atgcatgagc agggactggg aaccaagcag gatgtcgacg
     3121 aggccttcaa atggtacacc aaggccgcgg aacggggcga ccccatggcc aaacgtaaac
     3181 tcgctcaccg ccaggaaatc atcagcgaga taaagaatga acaggcggtc gagaagttga
     3241 aatcttctgc cacagcggac atacccgcga aaccggccga tgcattgagc cgctccacac
     3301 cgactgccgt agcgcagtcg gatcaggccc ggcagcagga gcgagacagg attaaagcga
     3361 ccgaaagaga caaaaaacgc gccatggtgc gcgccatgat tctcgaacgc atgcgccacc
     3421 ctgtgggcgc tccgtttggg gaataataca gctcgtcggg ccggttatta cgccgcaccg
     3481 ccgagacgtc tgggcgcggc cgcgcttttg acgatttcat ccaccagctt gtcgtcgatc
     3541 cacaaaaatg ccactcgcgt caccgcggaa acggtataca tgcgcggttt gagacaggag
     3601 atcgggttac tggccgtgga tgtaccaccc tcgatgtttt tcgacacgcc gtcagccgcc
     3661 actaattgca cgatgccatc gacgagatag agattccatg aatcgtttgt cccccgctcc
     3721 agtagctggg ttccgggcgg caccttatat accaagcatc gttcagcaag cacggcgagc
     3781 ttttcatggc cgagattgcg gaagcgggtg aatgccttga gtatatccgg ttcggctggc
     3841 tcaggacgac caaccagcga cgagctgatg cctctgatga aagcgctgtc gctggcatga
     3901 tttcccgcct gatctccggt tttcgagtcc agcgtaacct gacgctccga gcgcttgtcg
     3961 acgtcggcgt aagggtcatc tgtcggctcg cctttcgcga ccacggcgtt gcgacgaata
     4021 tcctcgatca aagtgaattc cgcggccttg cgcatgcctt tcttgacgat gccctgataa
     4081 atcttttcct tgagaatggt aatctcgggt gtataacgcc tgccgaccgg tatgccgttc
     4141 agcagattga gaatttccac caccggtgtc tcccggtcgg ccgacgttac cggccgatcg
     4201 aggttcgccg cataatcgtc cagcgtcgta tctcggtcgt aaagcacggg acaataaacc
     4261 ggcagatcgg gagtagaggc atccttgtat tcaaggaaaa tctccatgcc gcgataaatg
     4321 cttttttccc gattcttctt ctggaaggcg gtcagatcca gttccgtcgt tccggtcatg
     4381 ggataaagcc ggcgtacaat gcttttttca agcaacagcg tctcggaacc ccatggccgt
     4441 ttctcctgcg tccggtgaat gatggccaga cggaaatttc ccttctccgg actctgtaaa
     4501 taagtcatca ggaaacgccg gttataatcg gcaaacgcct tggtgagttt ggcgtccacg
     4561 acaaatttcc cgtccgggtc ataagcgcca agctcaccgt cggatgtcag cacatagaac
     4621 ccggtcaatc cggaaaatgt tttaatcgtt gccatttttc atttttaccc aaggacagag
     4681 atttcgccgg tcatcaacct gaaacacaaa atccgcaaac tgccggcagc ggcttcgcga
     4741 tactgatccg gcccgttctc caccgtttca atcacctcaa tgcttaagct taggtaaaag
     4801 caaggaatat gccaggaaaa ccatgatttg ccgtcaattt cccccaagag gcataaccgg
     4861 aaaggcccgg ataactaaag gaaattcctg gaaggcaacg agcaagggta cgtgatgctc
     4921 aggcgaaaat gccaatctgt ctccaaggcg cccaaacggg gcatggtcac caaaagacac
     4981 atgagcttga gagtggatgg gacgaaacta gatattccga tgtcgtcgct gtggagttta
     5041 tggatcttgg tggctcagat catgaaatgc atgcgtatat gccagacaaa caaagcccgc
     5101 ccaaagattg ccctgtataa tccattggaa tccttcatcc agaccccata aaaagttttt
     5161 aatcgtacaa ggaaatcatg tctttcattc aacaggccgt cctggccaaa aaagcagcgc
     5221 tcgccgaggt tgtcagtgaa ccgatggcgc ggttgggtaa ccgttgtgcc gaagtctggc
     5281 ccaacgcgga cgcgcttgat cgtctcctgc gcgaagcgat tgggtccatc ccccactgtc
     5341 atctgctcta tgcctgggat ctcaacggca ttgaaatatc gtccatggtc caggccagcg
     5401 gcgccgaccc ttcctggcgc ggccgcgatt tgtcgcaacg gccctatctg aaaaaccacc
     5461 tgccattcaa gggcgtgatg ctgtcatcgg tatacatgag ccagtacgtg ccgagacagt
     5521 gcatcaccgc gttgcaagcc gtctcgcgcg ataatcagct gctcggtttc atcgccgcgg
     5581 atttcgcgct ggacgacctg ctgcacgact cgcacctgac ggcgcccgac atgcactggc
     5641 agcagttccg cggcgacccg gcggtgcgcg gcacggtgtt catgcaggag cgcgtaccca
     5701 gcctgctgga tcagcatatc gacgaggtgc acgatctcat tcatcgcctc atgagccacc
     5761 acggggtgtt ccacagcaag attcattttt ccagcgggcg ctgctcgctg tggctgttta
     5821 acgaccccta taattaccgg ttgcaccggg tagacgagat catcaatccg gacttctgtc
     5881 tggcctaccc ggtctgcccc tacccggaca acgccaaggt ctcgccggac cagatccgtc
     5941 atgtgctgga cgagttcaag gccctgcgtt tcgccgatga aaccatttac ctgcgctcca
     6001 gctctctcaa tatcatgaac ggcctggtgg gcctcacctt ctcctgcgac ggctcgcatt
     6061 acatgccggt cgaggaattc atggagaagg acctgtcctt ctggctcggc tcgctcaata
     6121 tcaagccgga agccgccgcc gtttgagcca cctccgcagc gtccgggcgc gtcgcctgcc
     6181 ggacacctgc cgtggcgcgg tgtacgtctg gcggcttcct tcccggattt gtctatactt
     6241 gaaattgggt gtttgcaaaa aacacccgct tgagtctacg gatgtccggt acctcgaaca
     6301 tgcgtcaacc gaactggagg tagacgatga agacggtaaa cttgcagctg atcaccattg
     6361 gtctggagaa tgggcaacag ggggttttcg ttggcatccc cctgatcacg gaggaaaata
     6421 gcgacttgga cagtcaggtg gaggaaatct ggttttccaa tattcaggaa attccggacg
     6481 atctcagcgt caagaaactc atgcatctga tcgcgagcca gctctgccgg tgtagagcct
     6541 cgctgcagta acggcggttc gagtttttcc tgtttttccg ggccgggcgt gccggtctga
     6601 agtgtctttt tccctcacat ccctcagcac aagccgtcag cacggaacaa gcctgccaat
     6661 gttttggcat ccgtgatctc gcccgctcgc gcctgcatca gtgcctccgc aaatggaacc
     6721 caatgcacct cgaaaacctc atgcgcctcg gcgttggccg gcgtgccacg gagatcgcgc
     6781 gccagaaaca gatgcacgac ctcggtgaaa acaccgggcg agctgatgat tttacccagg
     6841 ggttcccagc gagcggcgcg caaaccggcc tcttcctgca gctcacgctg cgcggtcacg
     6901 agtggcggct caccggggtc gagcttgccg gcgggcaatt cccacaacca tccgcccgct
     6961 gcatgccggt actggcgcaa cagacatacc cggttctcag aatcgagcgc cacgacagcc
     7021 gccccaccgg gatgacgcac gatctcgagc tcggccatgg cgccattggg gagcgtgacg
     7081 cgctccagaa aaaaatcgat gatttttccg cggtatatga ccgtcttgct gtctgacaca
     7141 tttcctcccg ttggcgtgaa accgaatgtc ataatggcat tctaacggca aaaccggttc
     7201 atggccgcct cacatggcct ggcgccagaa agcgccgcgg gtaaaatcgg caagccgtct
     7261 tcaaccggga cataaacgga accaaggaaa tcatgcgccg ccctatattc atgtcgcttt
     7321 tcggcgccac gctggccggg atgtttctcg gaagctgcgc cacggtgcca gaaactggac
     7381 gttcacaact gttgatcgtc agtccggcgc aggaaacaca gctcggtttc caggaattcg
     7441 aaaaactcaa gaagtccctg cccatctcca aggacgcggc agccaatgcg caattgcagc
     7501 gcgtcggaag ccgcatcgcc gccatagcac cgctgcccaa tgcgcgctgg gagttcgtgc
     7561 tgttcgacaa acccgatacg cccaacgcgt tctgcctgcc tggcggcaag gtcggcgtct
     7621 tcagcggcat tttgcccatc accaaggacg acgccggact cgcgactgtg atcggacacg
     7681 aagtggcgca cgcggtggca cgtcatggtg cggagcgcat gtcgcaagat accgtatttc
     7741 agatacttga agagctcgcg ctgggacgct catcgcaggc aagccgttcg gtcatacaac
     7801 aggcctatgg cctcggattc gccctcccgc attcgcgcca acaggaactg gaggctgacc
     7861 atctcggtgt gctgtacatg gcgcgcgccg gctatcagcc gcgcgaagcg ctcgccttct
     7921 ggaaacgctt cgcggaatac aagaacaaga acggcggcgg aaaacaggtt gaatttctca
     7981 gcacgcatcc gcttgatgaa cagcgcatcc ggcagatcga agggctgatt ccacaagccg
     8041 agatggaata ccgcaaaccg gatgtacgat aaccatgggt tttctgtcat tattccggat
     8101 cgaattctga tattttttat cccgttgaaa atcctcctgc ctgcctcatt atagaaacct
     8161 gattttgaca gcacgtgcca gcgacacgcc gacagggatc aataatgctt tacctcggcg
     8221 gccattgcgg gcgcgaagga ggcaccatgt ttcatcgcag attgtatttc atcgttcccg
     8281 acgaagccca ggccgcgcaa atcgtgactg atctagaagc agcgggcgtg gggcgcaggc
     8341 acattcacgc gattggtggc aagggcgtga cactcgaaaa attgcctgcc gccaacgagc
     8401 gccagcaaca cgataccgtc tggcgcctgc aacagatcct gtggctggga aatcttggat
     8461 tgtttgtgct gggcgcgatc ggtctgatcg tttccgtcat acaagactcc ctgaccgggg
     8521 ctgttctggc gttcgccgcg atggcgctca cgctcacggc aggtgccctg ttcgctatac
     8581 gcgtgccgga cacgcatctc gatgagtttc gcggcgtgct ggcgcaccac gaagtgctgc
     8641 tcatggtgga cgtgccgaaa caaagagtgg cggagatcga ggagctgata tatcggcgtc
     8701 atccggaggc aacggcaggg ggcacaggct ggaccatcga ggcgctacag atataaatcg
     8761 ggcttatata tgcatcggat gtgcggtcat ggtcgtggaa aggcacatga aaaatagcgt
     8821 tgtgcccatg acagcatgag cgtatagctg taaatcagat caatgctttg tctcaggagg
     8881 agaccgtcat gttcaatatc gtttgcagaa cagaccctat cagcggcaag gatcttttca
     8941 ataccgccgg cttgccatac gtcacgcacc gcagcgacga aaccagcctt acgatttatt
     9001 tcgagtcgga gaacaaccgg cacatctatc tggaaatgcc ggtggagcac ccgaccagcg
     9061 agctgtccgt gaacctggac aatcccacgg acgaaatgat tgatgaaggt tgaacgaatc
     9121 ggggccagag gaaggcttgc agaggtcgcg ctcgcttcaa cgacgtccgt ggcggatcag
     9181 taattgccga cggtaagatc gcgcgccagg atgatcgcat cctcgcggcc atggcgcgcg
     9241 gggtaatagc ttttgcgcag gccgacctca tcgaaaccga gcttggtgta aagccggtaa
     9301 gcggcggtgt tggacatgcg cacttcgagc agcgccacac gcgccttgtt ctggcgggcg
     9361 atatccagca ggaacagcac catgccct
//
LOCUS       DBNGZE010000023         2061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1531493, whole genome shotgun sequence.
ACCESSION   DBNGZE010000023 DBNGZE010000000
VERSION     DBNGZE010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1531493"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..1238)
                     /locus_tag="AC16FV_00670"
     CDS             complement(414..1238)
                     /locus_tag="AC16FV_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2848607.1"
                     /translation="MSSRESLSPIRRILIALDASASGLAALETAVTLASRLEAELLGL
                     FVEDVNLLRFAALPFAREVSFFSARTRRLDSGEMERALRAQASRAEAALIEAAERQRV
                     RCSFRVVRGEVTTQLLAAAHDIDLVALGMTRRQFRRISSTVRGIVNAAQSSVLLLPPD
                     AQIRTPIVVVYDGSPASVRALSVARYLSQMQDSILTVLTTGKTGDPKVEVAEQLEGSG
                     LTVRYRSLPDVGIAGVVQSVQQEAIGTLVLCASLYLKEGTLEELLERVDCTVLLVR"
     gene            complement(1235..>2061)
                     /locus_tag="AC16FV_00675"
     CDS             complement(1235..>2061)
                     /locus_tag="AC16FV_00675"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR2848608.1"
                     /translation="DVQRALDARIYRADRLRERLQDEIHRGTLLIDTQGEHVGQVNGL
                     SVVALDDFIFGHPSRITASVRLGKGEVLDIQREIELGGPLHSKGVLILSGFLGARYAS
                     NRPLSLSASLVFEQTYGEVEGDSASSAELYALLSALAETPIKQSLAVTGSVNQHGEIQ
                     AIGGVNEKIEGFFDVCHRRGLTGEQGVLIPAANVKHLMLRRDVVETVAQEKFHIYPVS
                     TVDQGIELLTGLPAGVRDENGLFPDGSINQRVEVRLLILAEQARAYAAPLEVGKET"
BASE COUNT          463 a          625 c          569 g          404 t
ORIGIN      
        1 cccaccaagg tggtgcgcat cgcgcttgaa aacgccgtat cggtggcgag tgtgctgttg
       61 ctcaccgagg ccaccatgac cgagatcccg gaaaaggaaa aaccggcagg cagcgccgtg
      121 ccggaaatgt aggagtgaac catggccatc atcggtattg atctcggcac ttccaactcg
      181 gcggcggcgg tgctgcgcgg gaggggccct tcgcgccggt ggggacgacg taaatgaaaa
      241 tagccggacc agtcgtaacg ataagtctta tgactcctaa caaaaaatca gacaggattt
      301 acaggattag aaacacgatt aacaggatgg cttgtgtcta aaatctttaa tcctgttaat
      361 cctgtctata cactgtttaa gacggtattt tatgatcaag ctggaatttg ttctcagcgg
      421 acaagaagca ctgtgcaatc gacgcgttcc aggagctctt ccagtgttcc ttccttcaga
      481 tagaggcttg cgcataagac cagcgtgccg attgcctctt gctgcacact ctgtaccacg
      541 cctgcgatgc cgacatcagg caatgaacga taacgcacgg tgagtccgct tccttcaagc
      601 tgttccgcga cttccacttt cgggtcgcct gtctttcctg tagtcaggac agtcaggata
      661 ctatcctgca tttgcgagag gtaccgcgct accgacaatg cccttaccga ggccggcgaa
      721 ccatcataaa caaccacaat tggcgtgcgg atctgagcgt ccgggggcag aagcaacacc
      781 gaactctgcg ccgcgttgac gattccgcga acggtcgagc tgatgcgacg aaactgccgg
      841 cgtgtcatgc ccaaagcgac cagatcaata tcgtgcgccg cggcaagcaa ttgcgtcgtc
      901 acctctccgc gaacaacacg aaaagaacag cgtacgcgct gccgctcggc cgcctcgatc
      961 aacgcggcct ccgcccggga cgcctgcgcg cgcaacgcgc gctccatctc gccgctgtca
     1021 agacggcgtg ttctggcaga gaaaaagctc acttcacgcg caaagggcag cgcggcaaac
     1081 cgcagcaggt tgacgtcttc gacgaataac ccgagcagtt ctgcctccag ccgggaggcc
     1141 agcgttaccg ccgtctccag tgccgccagt ccggaagccg aagcatccag ggcgatcaat
     1201 atgcgccgga taggcgagag gctttcacgg ctgctcatgt ttccttcccg acttccagcg
     1261 gtgctgcgta agcacgcgcc tgttcggcca gaatcagcag ccttacttcc acgcgctggt
     1321 ttatcgagcc gtcaggaaag agaccgtttt catcgcgcac ccccgcagga aggccggtca
     1381 gcaattcgat gccctggtcg acagtgctta ccgggtaaat atggaatttt tcctgcgcca
     1441 ccgtctctac tacatcacgg cgcagcatca ggtgtttgac gttggccgcc gggataagca
     1501 caccctgctc accggtcagg ccccgacgat ggcaaacgtc gaagaaccct tcgatctttt
     1561 cattcactcc gccgatggcc tggatctcac catgctggtt gacggaaccg gtgacggcca
     1621 gtgactgctt gatgggcgtt tcggccagcg ctgacaacaa cgcatacagt tccgccgagg
     1681 aagctgaatc gccttcaact tcgccatagg tttgctcgaa cacgagactg gcggaaagcg
     1741 acagcggccg gttggacgca taacgtgctc cgaggaaccc cgagagtatc agcacgccct
     1801 tggaatggag tgggccgccg agttcgatct cgcgctgaat gtcgagcacc tcacccttgc
     1861 cgaggcgcac actggcggta atgcggctcg gatgaccgaa gataaaatcg tccagagcca
     1921 ccaccgacaa cccgttgacc tggccgacat gctcaccctg cgtatcaatg agcagcgtgc
     1981 cacggtggat ttcgtcctgc agtcgctcac gcaaacgatc ggcgcgataa atcctggcgt
     2041 cgagcgcacg ctgtacatca t
//
LOCUS       DBNGZE010000024         1907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1809543, whole genome shotgun sequence.
ACCESSION   DBNGZE010000024 DBNGZE010000000
VERSION     DBNGZE010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1809543"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC16FV_00680"
     CDS             <1..258
                     /locus_tag="AC16FV_00680"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR2848609.1"
                     /translation="RVRSALNRDDVQAQLIARGVDPAQVQARIDSLTDQEVQSLSGKI
                     NQLPAGGDSFLGALVFIFIVLLITDILGLTNVYPFVKHPKR"
     gene            326..733
                     /locus_tag="AC16FV_00685"
     CDS             326..733
                     /locus_tag="AC16FV_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR2848610.1"
                     /translation="METLRRLTKPVAHLVVLGMLALSLHLPAANAAMVGTETVVNAAQ
                     AQQSRERVLGTLNRNDVKAELMARGVDPAQVQARLDSLTDEEVQTLAAKMDQLPAGGD
                     GLGLLVFIFIVLLITDILGFTNVYPFVKHPQRQ"
     gene            755..1711
                     /locus_tag="AC16FV_00690"
     CDS             755..1711
                     /locus_tag="AC16FV_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HIR2848611.1"
                     /translation="MSRSRTARFLTGGFFVITALLTSGCASLHTDRILADADAFPRPV
                     ELTAVPFFPQEEYQCGPAALATVLNWSGVNISPVELAPQVYLPERQGSLQLELIGAAR
                     RHGRIPYVLQPQMESLLAEVSSGNPVLILQNLSLPWYPKWHYAVVVGFDLKRDYLVLR
                     SGREERHEVPFKVFERTWRRSDYWAMVVLPPDRLPFTAEEIPYVQAVAPLERLSRWPE
                     TAVAYATALTRWPKSLAARMGLGNSRYALGDVRGAEEAFRQATRDHPKSAAAYNNLAQ
                     TLLDQKRLPEAQAAAQRAVELGGPQSETFRETLKQIEAKLNP"
     gene            complement(1725..>1907)
                     /locus_tag="AC16FV_00695"
     CDS             complement(1725..>1907)
                     /locus_tag="AC16FV_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIR2848612.1"
                     /translation="QRLAGDAGEIIRDITRISEADLDASLADEEEARALTEIVEYLRV
                     GVQLVFEELQPPASKH"
BASE COUNT          358 a          617 c          573 g          359 t
ORIGIN      
        1 cgcgtgcgca gcgcattgaa ccgcgatgac gtgcaggccc aactcatcgc ccgcggcgtc
       61 gatccggcgc aggtgcaggc gcgcatcgac agcctgaccg atcaggaagt gcagtctctg
      121 tccggcaaaa tcaatcagct gccggccggt ggcgacagct tcctcggcgc gctggtgttc
      181 attttcatcg tgttgctgat caccgacatt ctgggtctca ccaacgttta tccattcgtc
      241 aaacatccaa agcgttaaac gtcagcatgc gttgctaccg tttgattcac gtttttactt
      301 aaactcgacc ttcaggaggt caaccatgga aaccctacgt cgcctcacca aacccgtcgc
      361 ccatctggtc gtcctcggca tgctggcgct cagcctgcac ctgcccgccg cgaacgccgc
      421 catggtcggc accgaaacgg tggtcaacgc ggcccaggcg caacagagcc gcgagcgcgt
      481 gctcggcact ctgaaccgca atgacgtgaa ggccgagctc atggcccgtg gcgtcgatcc
      541 agcacaggtg caagcgcgtc tcgacagcct gacggacgag gaagtccaga ccttggccgc
      601 caagatggat cagttgccgg ccggcggtga cggcctcggc ctgctggtgt tcatctttat
      661 cgtattgctg atcacggaca ttctcggatt caccaacgtc tatcccttcg tcaaacatcc
      721 gcagcgccag taatcgtcgt tggcgattgt ccgcatgagt cgttcccgaa ccgcccggtt
      781 tctcacgggc ggtttttttg ttatcacggc cctgctcacg agcggctgcg cctcgctgca
      841 cacggatcgc atactcgccg acgccgatgc ctttccgcga ccggtggagc tgacggccgt
      901 gccatttttc ccgcaggagg aatatcagtg cgggccggcg gcgctggcga cggtgctcaa
      961 ctggagcggc gtcaacatca gccccgtgga actggcgcca caggtttatc tgcccgagcg
     1021 tcagggcagt ctgcaactgg aactgatcgg cgccgcgcga cgtcacgggc gcatcccgta
     1081 tgtattgcaa ccgcagatgg aatcgttgct ggccgaagtc agctccggca atccggtgct
     1141 gatcctgcaa aacctgagtc tcccttggta cccgaaatgg cattacgccg tggtcgtggg
     1201 attcgatctg aagcgtgact acctcgtgct gcgttcgggc agggaggaac gacacgaggt
     1261 gccgttcaag gttttcgagc gcacctggcg ccgctcggat tactgggcca tggtggtcct
     1321 gccgcctgac cgtctgccgt tcaccgccga ggagattcct tacgtgcagg cggtcgcgcc
     1381 gctcgaacgc ctgtcacgct ggccggaaac ggcggtggcc tatgccacgg cgctcacacg
     1441 ctggccgaag agtctcgccg cgcgcatggg actgggcaac agccgctatg cgctgggcga
     1501 cgtgcgcggc gcggaagagg ccttccggca ggcgacgcgg gatcatccca agtccgccgc
     1561 agcgtacaac aatctcgcgc aaacgcttct ggatcagaag cgtttgccgg aggcacaggc
     1621 cgcggcacag cgcgcggtgg aactcggcgg accgcaaagc gaaaccttcc gcgaaactct
     1681 gaagcagatc gaggcgaagc tgaatccgta agaatgaaag acggttaatg cttcgaggcc
     1741 ggcggctgca attcctcgaa caccagctgc acacccaccc gcaggtattc cacgatctcc
     1801 gtcaacgccc gcgcctcttc ttcatcggcc agcgaggcgt ccaggtcggc ctcggaaata
     1861 cgcgtgatat cacggatgat ctcgccggca tcgccggcaa ggcgctg
//
LOCUS       DBNGZE010000025         2627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1253733, whole genome shotgun sequence.
ACCESSION   DBNGZE010000025 DBNGZE010000000
VERSION     DBNGZE010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1253733"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="AC16FV_00700"
     CDS             complement(<1..960)
                     /locus_tag="AC16FV_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2848613.1"
                     /translation="MAKSKNNNELNIAGCIARYFIDSKLTLLIMAFAFLAGVFAFLVT
                     PREENPSIIVPAANVIVQKPGASPTEIEQLIVKPLEAILQGLAGIDHTYGVAMNSQGV
                     VTVQFEVGQNKEDSLVKLYDRIMSNLDRMPLGTLQPLVKPADVDDVPILTISLSCTTR
                     DACELRRIANNVLEELRRVEGAAFSFVHGGEPRKINVYLDLEHLRAYNVTLADIRRVV
                     EATNIDIPSGAFVGGNRVASVQAGGALRSASDVGDLVVGLSQHRPVYLKQVARIVDGP
                     GEVERLHRIGYGPAFSGKRVADYETPAVTVAIAKRAGTNAVKVA"
     gene            complement(1148..1528)
                     /locus_tag="AC16FV_00705"
     CDS             complement(1148..1528)
                     /locus_tag="AC16FV_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848614.1"
                     /translation="MSIPQHVALAMLVALTFAGCDKKPPEVPPPPPRMERPALSLNIQ
                     PGELLKIKIPRAALVERGGIPGVFVLNEAGEARFRLVRPGRIGDAQVEILSGLHGNET
                     LLLGDLTAVHDGSPIIVLNADKRR"
     gene            complement(1525..>2627)
                     /locus_tag="AC16FV_00710"
     CDS             complement(1525..>2627)
                     /locus_tag="AC16FV_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2848615.1"
                     /translation="GGKLNTRNVTTQDFDPARLNHPDTSDLHTTQLALRLPVYAGGRL
                     SATLTSAEEMEKNARLQYERAREITAFQTRRAWLNVHSAQEGLAIADDAVKAAQEHAR
                     TTAQLSREGRIVVSDKLTAEVNLAAVQSQREQAATRLESARNQLKLIMGLPLETEITL
                     TPASFGVASDATNLTELEARALTNRQDLAGVRAMHQAAKARVQAARAAHKPSVDFIAT
                     SDWYDDNPGFDTHSSSIMGVVSLDLYAGGRHQGEIAAALAEEREAQWHVQSLEQAVRH
                     EVREAWENLREARVRHANAADNAERARENVRLVKQRYGQGRTILIDLLQAERAYTDAR
                     SEELVSRVGLEVGQAALQLAEGALPLPAEEAQ"
BASE COUNT          421 a          815 c          844 g          547 t
ORIGIN      
        1 ggcgaccttc acggcgttgg tgccggcgcg cttggcgatg gcaaccgtca ccgccggtgt
       61 ctcgtaatcg gcgacgcgct tgcccgaaaa tgccgggccg tagccgatgc ggtgcaggcg
      121 ctcgacctcg ccggggccgt cgacgatgcg cgcgacctgc ttcaggtaca ccggccggtg
      181 ctgggacagc cccactacca ggtcgccgac gtcggaggcc gagcgcaagg ccccgccggc
      241 ctggacgctg gcgacgcggt tgccgcccac gaacgcgccg gacggaatgt cgatgttggt
      301 ggcttcgacc acgcgccgga tatcggcgag cgtgacgttg taggcgcgca ggtgttcgag
      361 gtcgaggtag acgttgatct tgcgtggctc gccgccgtgc acgaacgaaa acgcggcgcc
      421 ctcgacgcgg cgcagttcct ccaatacgtt gttggcgatg cggcgcagtt cacaggcatc
      481 acgcgtggtg cacgaaaggg agatggtgag gatcggcacg tcgtccacgt cggccggctt
      541 gaccagtggt tgcagcgtgc caagcggcat gcggtcgagg ttgctcatga tgcggtcgta
      601 caacttgacc agcgagtctt ccttgttctg cccgacctcg aattgcaccg tgaccacgcc
      661 ctgcgagttc atggcgacgc cataggtgtg gtcgatgccc gccaggccct ggaggatggc
      721 ttccagcggc ttgacgatga gctgttcgat ctcagtcggc gaagcgccgg gcttctgcac
      781 gatgacgttg gccgccggca cgatgatcga ggggttctcc tcgcgcggcg tcaccaggaa
      841 tgcgaacacg ccggcgagaa aggcgaacgc catgatcagc agcgtcagct tgctgtcgat
      901 gaaatagcgt gcgatgcacc cggcgatgtt caactcgttg ttgtttttgc ttttagccat
      961 atgatctctg gaacgcagat gcacgcagat ttatatgtaa tgatgaacgc ggatgaatcc
     1021 ggtcaatttc gcaatatgct cgtgccatcc ctggcactcg ccctcgctca cgctcgggcc
     1081 agtgtcgctg tgctccactg tccaatttat tgctcccagc aaaattgtct gcgttcttcc
     1141 ataaaaatca tctgcgttta tctgcgttaa gaactattat cgggctgccg tcgtgcacgg
     1201 cggtgagatc gccgagcagc agggtttcat tgccatgcag cccgctcaga atttcaacct
     1261 gtgcgtcgcc gattctgccg gggcgcacca gccggaaacg cgcctcgccg gcctcgttca
     1321 gcacaaacac gcccggaatg ccgccgcgtt ccaccagcgc cgcgcgcggt atttttattt
     1381 tgagcaactc cccaggttgt atatttaatg acagcgccgg ccgttccatt cgcggcgggg
     1441 gcggtggcac ttctggtggt tttttatcgc aaccggcgaa cgtcagagca accagcatgg
     1501 ccagggcaac atgttgcggg atgctcattg agcttcctcc gccggcaagg gcaatgcccc
     1561 ttcggcgagt tgcaacgcgg cctgtcccac ctccagaccc acgcgcgaga ccagttcctc
     1621 gctacgcgcg tcggtgtagg cgcgttccgc ttgcagcaga tcgatgagta tcgtgcgtcc
     1681 ctgaccgtag cgctgtttga cgaggcgcac gttctcgcgc gcgcgttcgg cgttgtcggc
     1741 agcgttcgcg tgccgcaccc gtgcctcacg caggttttcc caggcttctc gcacctcgtg
     1801 acgcaccgcc tgttccagcg actgcacgtg ccactgcgct tctctttcct cggccagggc
     1861 cgcggcaatt tccccttgat gccgtccgcc ggcgtagaga tcgaggctga ccacgcccat
     1921 gatgctcgat gaatgggtat cgaaacccgg gttgtcgtca taccagtcac tggtagcgat
     1981 gaagtccacg ctgggtttgt gcgccgcgcg tgcggcctgc acccgtgcct tggcggcctg
     2041 gtgcatggcg cgcacccctg ccaggtcctg gcggttcgtc aacgcacgtg cttcgagttc
     2101 cgtgaggttg gttgcgtcgg acgccacgcc gaagctggcg ggggtcagcg tgatctcggt
     2161 ttcaagcggc agacccatga tgagtttcag ctgattgcgc gccgactcga ggcgggtcgc
     2221 agcctgctcg cgctgtgatt gcacggcagc gagattgact tcggcggtga gtttgtcgga
     2281 gaccacgatg cggccctcgc gtgaaagctg cgccgtggtg cgcgcgtgtt cctgcgcggc
     2341 cttgaccgcg tcgtcggcga tggcaagccc ttcctgggcg gagtggacat tcaaccatgc
     2401 acgccgggtc tggaaggcgg tgatctcgcg cgcgcgttca tattgcagcc gggcgttttt
     2461 ttccatctct tcggcactcg tcagcgtggc ggaaagccgc cccccggcgt acaccggcag
     2521 gcgcagcgcc aattgcgttg tatgcaggtc gctggtgtcc ggatgattca ggcgcgcggg
     2581 atcgaaatcc tgcgtggtga cattgcgcgt gttgagcttg ccgccga
//
LOCUS       DBNGZE010000026         9332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1809688, whole genome shotgun sequence.
ACCESSION   DBNGZE010000026 DBNGZE010000000
VERSION     DBNGZE010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1809688"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16FV_00715"
     CDS             <1..399
                     /locus_tag="AC16FV_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR2848616.1"
                     /translation="VEQLFDETLVLVSSRPDDKAPGEDYIYVEWGPGFYAQHAQSYPD
                     LEGPAQIVDIGWLGIQLALTNDGSCFLPARMAASFIAAGRLFRVAGSPEYVLPAYVVY
                     PRSSDSPVLQQALQGLRELAGLERERAARG"
     gene            481..897
                     /locus_tag="AC16FV_00720"
     CDS             481..897
                     /locus_tag="AC16FV_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR2848617.1"
                     /translation="MTQSILRGGCLCGSVQYEISGDPQRFYHCHCARCRKATGTGHAS
                     NLLIKPGSLKWIKGEELIRCFKVPEAKRFTNCFCSTCGGRVPRYVKETDMILIPAGSL
                     DSEPPIHPQARIFWGSRASWSCGGDELPVYPEYPIS"
     gene            1034..1834
                     /locus_tag="AC16FV_00725"
     CDS             1034..1834
                     /locus_tag="AC16FV_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR2848618.1"
                     /translation="MQNIKSLIRIPVVLALAAFSLAAQADHSWNGYHWARTTSYFTLR
                     VVDSVTTDWDGQLSESLTRWSYSSVLAANVTSADDSTKTRKICRMVQGQMRVCNAAYG
                     FNGWLGLATIGLDPNGHIDRGTAKMNDSYSSYWTVEEKNHVMCQEIGHVLGLGHTSED
                     GSSQQTCMDYSSDPLSQWPNQHDYDQLLAIYGHTDSYNSYDDGSGGGGGACTAPPGKG
                     CNKNGVVDVPPMGVRVVRGPHHEIWVAPRGDGGLWIHHIRLAPQELRN"
     gene            2006..2893
                     /locus_tag="AC16FV_00730"
     CDS             2006..2893
                     /locus_tag="AC16FV_00730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2848619.1"
                     /translation="MRLGFIGLGNMGSEMARHLLAAGHTLSTYTRGERSRTHASRLGL
                     SLATSPAAVAQASEVVFTMVTAGRDVEAVALGQDGIVHGASPGLIHVDMSTISPAIAR
                     DVAKCLSERRIAMLDAPVSGGVAGAKTASLTFFVGGDQTVFERARPLLECMGKTIFHM
                     GHLGTGQVTKLANQIAQLACLQGAAEALLFAREQGADAGKVREAVMTGLGASRMLDTL
                     GKKMVERDFAAGIVAALHHKDIGVALELAHDAGLPMPITAQVMQQLNALMGSGLGEQD
                     TSSLLLVLEQMRGQKLQKS"
     gene            3095..4156
                     /gene="nadA"
                     /locus_tag="AC16FV_00735"
     CDS             3095..4156
                     /gene="nadA"
                     /locus_tag="AC16FV_00735"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR2848620.1"
                     /translation="MNQRVEIPAPVAVKLPATPPPPPLSDEERMRLRERVKRLLQERD
                     AVLVAHYYVDEDLQRLAEETGGCVADSLEMARFGHDHPARTLVVAGVRFMGETAKILN
                     PEKRVLMPTLQAECSLDLGCPASLFIPFCDAHPDRQVVVYSNTSAEVKARADWVVTSS
                     IAVKLIEHLKSRGEKILWAPDRHLGKYLQSVTGADMLLWNATCVVHDEFKGEALAALK
                     RQHPDAAIIAHPESPTAVLAQAEVIGSTSQLIAAAKRLPNKRLIVATDRGIFYKMRQA
                     VPDKILMVAPTAGEGATCESCAHCPWMAMNALTNLAEVLESGANEVHVEEAVRVKALR
                     ATQRMLDFAASIRQKNHGD"
     gene            complement(4399..5052)
                     /locus_tag="AC16FV_00740"
     CDS             complement(4399..5052)
                     /locus_tag="AC16FV_00740"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR2848621.1"
                     /translation="MSHRYPISIPAVSTALKSKLKALAADALKPRCAWCGADPLYVRY
                     HDEEWGVPVHDERKHFEFLILEGAQAGLSWITILRKRENYREAFAGFNPGRVARFGKR
                     EVAHLLKNPGIVRNRLKIESAVKNARAFLAVQDEFGGFDNYVWRFVGGHPIRNRWTDI
                     KQIPARTAESDALSKDLKKRGFTFVGSTIIYAHMQAVGMVNDHVVSCFRYKQPARRR"
     gene            complement(5153..6967)
                     /locus_tag="AC16FV_00745"
     CDS             complement(5153..6967)
                     /locus_tag="AC16FV_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HIR2848622.1"
                     /translation="MALRPKAMRWFEVYVPRYETVRALGVLARTGKVELDTNPKVNAS
                     LEIGEINNALQEFARIASRYRAYWPKGEYCPVELPLSPLDTAKQTLEQLRAWGREVDA
                     NIDRIRKLEREKHNLEHLQVLFEQPGEEHLDLSRVAAPMQLLYIGLGECPIDEDVSML
                     PDGALVKNIPANDRRFVMAAGLPEHAGEIESAMAAHGCQPLTSPPWLTADHEENLRRV
                     RARLVAIDHEQMGMIEKLDKLAKQRGLVEALGNIGVLKWYVEHAPEVEMGTKFCHVTG
                     WTAEPDESRLNEAFRLADIHAMIRFPPEAATEQPTGVENPWWVQPFEFFTQMVGTPGR
                     AEVDPSGLLAIIVPLLFGYMFPDVGHGLILILTGIVFARHWPPITFLIPCGVASIGFG
                     FVFGEFFSFDTVISALWIKPLDNPMAVLAVPLIGGVVLMLLGILLASIEARWRGELGT
                     WLAVDAAVMLMYAAALIALFYPVVWPGVAIGAAWYFIGGFYVRRKRPFQRLLELFGRL
                     LESVFQLFINTISFARVGAFALGHVALSSVLMTLAERIENPGTYAVVIVLGNLFIVAV
                     EGLMVFVQTTRLILFEFFSRFLRAEGRIFKPLRRPTSA"
     gene            7074..7313
                     /locus_tag="AC16FV_00750"
     CDS             7074..7313
                     /locus_tag="AC16FV_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848623.1"
                     /translation="MKKSNCFGSVYYLRTLWIAGFLAVAGLAAGSTLAADDTSLDDSA
                     KKVGNNFGELLKGMGKELKKAGGSLTESGKKDGKK"
     gene            7412..8422
                     /gene="ahr"
                     /locus_tag="AC16FV_00755"
     CDS             7412..8422
                     /gene="ahr"
                     /locus_tag="AC16FV_00755"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008106 - alcohol dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HIR2848624.1"
                     /translation="MIQAYAVQKAKGRLKPIEYDPGALGADQVEISISACGLCHSDLS
                     MIDNEWGDSEYPLVPGHEIVGKIAQVGTNVKHLKIGDTVGLGWFSGSCLTCHACLSGD
                     HNLCADAEQTIVGRHGGFADKVRCQATWATPLPAGLDVIKAGPLFCAGITVFNPLVQF
                     DVRPTQRVGVIGIGGLGHLALQFLNKWGCEVTAFTSTDAKREEALRMGAHHIVNSRHA
                     AQLMKIAGSLDFILSTVAVDLDWDAYLDALAPKGRLHIVGVIPSPMEVTADVLIGGQH
                     SLSGSPTGTPAMLRTMLDFCARHRIEPVTEIFPMSRVNEAISHLRAGKARYRVVLEND
                     FG"
     gene            8587..8820
                     /locus_tag="AC16FV_00760"
     CDS             8587..8820
                     /locus_tag="AC16FV_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848625.1"
                     /translation="MSMLKKLSILSVGLMIAVGLVACEQKGPAEKVGEKIDQTVEKAK
                     EKVEDVTKPEGPMEKAGKKIDQAVEDAKEAIKK"
     gene            complement(8972..>9332)
                     /locus_tag="AC16FV_00765"
     CDS             complement(8972..>9332)
                     /locus_tag="AC16FV_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HIR2848626.1"
                     /translation="HGQWRWGAAAPGAAQHLCPACQRIHDRVPAGELTLSGKFFAEHR
                     EEILHLVRHAESKARAEHPLERIMDIKDEADHTVVTFTDSHLVHGIGEALRHAYHGEL
                     ESQYTDEGDLLRVTWSR"
BASE COUNT         1875 a         2860 c         2879 g         1718 t
ORIGIN      
        1 gtcgaacagc tcttcgacga aacgctggtg ctggtcagct cgcgcccgga cgacaaggcc
       61 ccgggcgagg actacatcta tgtcgaatgg gggccgggct tttatgccca gcatgcccag
      121 agctatcccg atctcgaagg accggcgcag atcgtcgaca tcggctggct tggcatccag
      181 ctcgcgctca ccaatgacgg cagctgcttt ctgcccgcgc gcatggccgc gtccttcatc
      241 gccgccgggc gtttgtttcg tgtcgcagga tcgccggagt atgtcctgcc cgcctatgtg
      301 gtttacccgc gaagcagcga cagcccggtg ctgcaacagg cgctgcaagg gctgcgggaa
      361 ttggcggggc tggaacgcga gcgcgccgcg cgcgggtgat cgaacggaac ggaagggtcg
      421 ttttaccggc ggtccgcatg agataacctg tttccacata caacattcgg cggaaagtcc
      481 atgacacagt ccatcttacg cggcggttgc ctctgcggtt ccgtgcaata tgaaatcagc
      541 ggggacccgc aacggtttta tcactgtcat tgcgcgcgct gtcgcaaggc cacgggcacg
      601 gggcatgcct ccaacctgct catcaagccg gggtcgctca aatggatcaa gggcgaggaa
      661 ctgatccgtt gtttcaaagt ccccgaggcg aagcgcttca ccaactgttt ttgctcgacc
      721 tgcggcggcc gagtgccacg ctacgtgaag gagacggaca tgatcctcat cccggccggc
      781 tcgctcgaca gcgagccgcc gattcaccca caggcgcgca tcttctgggg ttcgcgcgcg
      841 agctggtcct gcggcggtga cgagctgccg gtttatccgg aatacccgat cagctaaata
      901 caggtgggcc caacgcactg ttttccaagc tgaaaccatg cgccgaggct gttctccgcc
      961 ggtcgttttt tgacttcgcg tgaccatacg ccatacgatg ttgctgaaga acaatatcca
     1021 ctgggaggct tgtatgcaga acatcaaatc gctgattcgc attccggttg ttttggccct
     1081 cgcggcgttc tcgctcgcgg cgcaggccga tcactcatgg aacggttacc attgggcgcg
     1141 tacgacgagt tacttcacgc tgcgggtcgt ggacagcgtg acgacggatt gggatgggca
     1201 gttgagcgaa tcgctcaccc ggtggtccta ctcgagcgtt ctggccgcca acgtaacctc
     1261 ggcggatgat tcgaccaaga cgcgcaagat atgccgcatg gtgcaaggcc agatgcgcgt
     1321 ttgcaacgcc gcctatggtt ttaacggatg gctggggctt gccacgatcg ggctcgaccc
     1381 gaacggtcac atcgaccggg gcacggccaa aatgaacgac agctactctt cctactggac
     1441 ggttgaggaa aagaatcacg tcatgtgcca ggaaatcggc catgtcctgg gcctcggtca
     1501 cacgagcgaa gacggcagca gccagcagac ctgcatggac tattcgtcgg atccgctcag
     1561 ccagtggccg aaccagcacg actacgatca actcctcgcc atttacggcc acaccgattc
     1621 ctataacagc tatgacgatg gatcgggcgg tggcggaggc gcatgcaccg cgccgccggg
     1681 caagggctgc aacaagaacg gcgtggtgga tgtcccgccc atgggcgtgc gtgtcgtgcg
     1741 cggcccgcat catgaaattt gggtggcgcc ccggggcgac ggcggcttat ggatacacca
     1801 catccgactc gctccccagg aattgagaaa ttaacaggga tcggagcgtt tgattttcag
     1861 caaccgccgc acatataaat acagcgacaa ccaaggcccg gcccatgccg ggcctttttg
     1921 ttttccttca cgccccgaac cgcttcacgc gatcccgggt aaataaggta aggttcgctt
     1981 tctccgtcag cagaaggtga aacccatgcg actcggattt atcggcctcg gcaacatggg
     2041 cagtgagatg gcgcggcatc tgctcgccgc cggccatacg ctcagcactt acacgcgcgg
     2101 cgaacgctcg cgcacccacg cgagccgctt gggattgagc ttggcgacct cgcccgccgc
     2161 ggtcgcgcaa gcgagtgagg tggtgttcac catggtcacc gccgggcgcg atgtcgaggc
     2221 ggtcgcgctc ggacaagatg ggattgttca cggcgcatcg ccgggattga tccacgtgga
     2281 catgagcacg atctcgcccg cgattgcacg ggatgttgcc aagtgcctga gcgaacgccg
     2341 cattgccatg ctggatgcgc cggtttcggg cggtgtggcg ggtgccaaga cggcatcgct
     2401 caccttcttc gtcggcgggg atcagaccgt cttcgaacgc gcgcggccgc tgctggaatg
     2461 catgggcaag acgatctttc acatgggtca cttgggaacc ggacaggtga ccaagctcgc
     2521 caaccagatc gcccagctcg cctgcctgca gggcgccgcc gaggcgctgt tgttcgcgcg
     2581 cgagcagggc gcggacgcgg gcaaggtgcg cgaggcagtc atgaccgggc tgggcgccag
     2641 ccgcatgctc gatacgctgg gaaaaaagat ggtcgagcgc gatttcgccg ccggcatcgt
     2701 cgccgctttg catcacaagg atatcggtgt cgcgctcgaa ctcgcgcacg acgccggttt
     2761 gccaatgccc atcaccgcgc aggtgatgca gcagctcaac gccctcatgg gtagtggcct
     2821 aggcgaacag gacacgagct cgctgttgct ggtgctggag cagatgcggg gacaaaaatt
     2881 acagaagtcg tgatttgctt taagtctgcc gccgcagagg cacggataca cacaccattt
     2941 tgatggctaa agcgggcgca caccttgccg acgattgaac acaattaccg ccgggtgatt
     3001 taataacgac gaagtgagtt gaatttcagc gcttcgcccg caaaacaaaa agaaatacag
     3061 gctcccgact gggactggga gccggaggta gaacgtgaac cagcgagtcg agattcccgc
     3121 gccggtggcg gtcaagctgc cggcaacgcc gccacccccg ccgctgtcgg acgaggaacg
     3181 gatgcgcctg cgcgagcgtg tcaagcgcct gctccaggag cgcgacgcgg tgctggtggc
     3241 gcactactac gtggatgagg acctgcaacg tctggccgag gaaaccggcg gttgtgtggc
     3301 ggactcgctc gagatggcgc gcttcggcca tgaccaccca gcgcgtacgc tggttgtcgc
     3361 cggggtgcgt ttcatgggcg agaccgcgaa aatcctcaac cccgaaaagc gcgtgctcat
     3421 gccgacgcta caggcggagt gctcgctcga cctcggttgc ccggcgagtc tgttcattcc
     3481 cttttgcgac gcccaccccg accgtcaggt ggtcgtgtat tcgaatacca gcgccgaggt
     3541 caaagcgcgc gccgactggg tagtaacctc gagcatcgcg gtcaagctca tcgagcattt
     3601 gaaatcccgt ggcgagaaaa tcctgtgggc cccagacagg cacctcggca aataccttca
     3661 aagtgtcacc ggtgcggaca tgctgctctg gaacgccacc tgcgtggtgc acgacgagtt
     3721 caagggagag gcgctcgcgg cgctcaagcg ccagcatccc gacgccgcga tcatcgcgca
     3781 tcccgaatcg ccgacggcgg tgcttgcgca ggccgaggtt attggctcga ccagccagct
     3841 gatcgcggcg gccaaacgcc tgccgaacaa gcgtctgatt gtcgccaccg atcgcggaat
     3901 tttctacaag atgcggcagg cggtgccgga caagatcctc atggtcgccc ccaccgcggg
     3961 cgagggcgcc acctgcgaga gttgcgcgca ctgcccgtgg atggcgatga acgcgctcac
     4021 caatctggcc gaagtgctgg agagcggcgc gaacgaggtg cacgtggagg aggcggtgcg
     4081 ggtgaaggcg ctgcgcgcca cgcagcgcat gctcgacttc gcggcctcga tccggcagaa
     4141 aaatcatggc gattgacggc accgtttcga gggttgggct ggtttatccc cgatcgatta
     4201 tttccgctac gtcccccact gcaagcctgc ccccgctgac aacgcgagga tgccgcggcg
     4261 gcactaggga acctctgaac aagtccgttc gtggtgagcc tgtcgaacca taaacggacg
     4321 aatcgcccgt tatttacggt atttcccgtt cacccttcga cgagctcagg gcgaacggga
     4381 gacttaatca gaggttccct agcgccgccg cgccggttgc ttgtagcgga agcagctaac
     4441 cacatggtcg ttgaccatgc cgaccgcctg catgtgggcg tagatgatcg tcgagccgac
     4501 gaaagtgaaa ccgcgtttct tgagatcctt gcttaacgcg tccgattccg ccgtgcgagc
     4561 cggtatttgc tttatgtcgg tccagcggtt ccggatcggg tggccgccga cgaagcgcca
     4621 gacgtaattg tcgaaaccgc cgaactcgtc ctgcaccgcc aggaaagcgc gcgcgttctt
     4681 caccgcgctt tctatcttca ggcggttgcg cacgatgccg gggttcttca gcaggtgcgc
     4741 cacctcgcgc ttgccgaagc gcgcaacgcg cccggggttg aaacccgcga atgcctcgcg
     4801 gtagttctcg cgcttgcgca ggatggtgat ccaggacagt ccggcctgcg cgccttccag
     4861 gatcaggaac tcgaagtgct tgcgctcgtc gtgcaccggc acgccccatt cctcatcgtg
     4921 gtagcgcaca tacagggggt cggcgccgca ccatgcgcag cgcggtttga gggcatccgc
     4981 cgcgagcgcc ttgagcttgc tcttcaaggc ggtgctgacg gccggaatcg aaatcgggta
     5041 tcggtgcgac atggctggtc tccagccggc gaagatagcc gatcgggcat cgggtgggaa
     5101 tatcggcaaa acaaccgtca aagcggcgcg cctgagggtg cgcacgcccg gctcaggcgg
     5161 acgtcggccg gcgcaacggt ttgaagatcc ggccctcggc gcgcaggaaa cggctgaaga
     5221 actcgaacag gatcaggcgc gtggtctgca cgaacaccat caggccttca acggcaacga
     5281 tgaacaggtt gcccagcacg atcacgacag cataggtccc ggggttttcg atgcgctcgg
     5341 ccagcgtcat cagcacgctc gacaacgcca cgtggccgag ggcgaacgcg ccgacgcgcg
     5401 cgaacgagat ggtgttgatg aacaactgga acacgctttc cagcaggcgg ccgaacagtt
     5461 ccagcaggcg ctgaaacggc cttttgcggc gcacataaaa accgccgatg aaataccacg
     5521 cggcgccgat tgcgacaccc ggccagacga cgggatagaa cagcgctatc aacgcggcgg
     5581 cgtacatgag catgacggcg gcgtccaccg ccagccaggt ccccaactca ccgcgccagc
     5641 gcgcctcgat ggaggccagc agaatcccga gcagcatcag taccacgccg ccgatcagtg
     5701 gcacggccag caccgccatt ggattgtcca gcggcttgat ccatagcgca gagatgacag
     5761 tgtcaaagct gaagaattcg ccgaacacga atccgaatcc gatcgacgcg acaccgcagg
     5821 gaatcaggaa tgtgatcggc ggccagtgcc gggcgaacac gatgccggtg aggatcaaaa
     5881 tcaggccgtg cccgacgtcg gggaacatgt agccgaacag cagtggcacg atgatggcca
     5941 gcaggccgct cgggtcgacc tcggcccttc ccggcgtgcc gaccatctgc gtaaaaaatt
     6001 cgaaaggctg gacccaccag ggattctcca cgccagtcgg ttgctccgtc gccgcctccg
     6061 gcgggaagcg gatcatggcg tggatgtccg ccagccggaa ggcctcgttc agccgcgatt
     6121 cgtcgggttc cgcggtccag cccgtgacgt gacagaactt cgtccccatc tccacttccg
     6181 gcgcgtgctc gacgtaccac ttcagcacgc cgatgtttcc cagcgcctcg accagaccgc
     6241 gctgcttcgc gagcttgtcg agtttctcga tcatgcccat ttgttcgtga tcgattgcca
     6301 cgaggcgcgc gcgaacccgc ctcagattct cctcgtgatc cgccgtgagc cacggcggac
     6361 tggtcaacgg ctggcagccg tgcgcggcca tggcggattc gatctcgccg gcgtgttccg
     6421 gcaggccggc tgccatcacg aagcgacggt cgttggcagg gatgttcttc accaatgcgc
     6481 catccggcag catcgatacg tcttcgtcga ttgggcattc accgagaccg atatagagca
     6541 gttgcatcgg cgccgcgacc cgtgacaggt ccagatgttc ttcgcccggc tgctcgaaca
     6601 gcacctgcaa atgttcgagg ttatgcttct cccgctccag cttccggatg cgatcgatgt
     6661 tcgcatccac ctcccggccc cacgcgcgca gctgttccag cgtctgcttc gccgtgtcca
     6721 gcggcgacag tggcagttcg acgggacagt attcgccctt tggccagtac gcgcggtagc
     6781 gcgacgcgat cctggcgaac tcctgcaacg cgttgttgat ttcgccgatc tcgagcgagg
     6841 cgttcacctt ggggttggtg tccagttcca ccttgccggt gcgcgccagc acgcccaatg
     6901 cgcgcacggt ctcgtagcgt gggacgtaaa cctcgaacca gcgcatcgct ttaggtcgca
     6961 gggccacgct caggctccgg aaagaaaggc ttgacccatc atagcaccac aggattcacc
     7021 gcccgatgca atatatggaa taattggatc gtcgggtcac ctgatcgaat ctcatgaaaa
     7081 aatcgaattg ctttggtagc gtgtactatt tgcggacgct gtggatcgcg ggcttcctcg
     7141 ccgttgccgg tctcgcggcc ggttccactc tggcggctga tgacaccagt ctggatgaca
     7201 gcgccaaaaa ggttggaaat aatttcggcg agttgctcaa gggcatgggc aaggaattga
     7261 aaaaagccgg tggatcgctc accgagtcgg gaaaaaagga cggaaagaag taaaagaagc
     7321 gcccggagca tgaaccggat aaggacaagg acagcgccaa gtaatcctcc ggccacgacg
     7381 cgcttgatgg tcgcccggta aggagagcct catgatccag gcctacgcgg tccagaaagc
     7441 caaaggccgc ctgaaaccca tcgagtacga ccctggcgcg ctcggcgccg atcaggtcga
     7501 gatcagcatc agcgcctgcg gcctgtgtca ttccgatctg tccatgatcg acaatgaatg
     7561 gggcgatagc gagtatccgc tcgtccccgg ccatgagatt gttggcaaga tcgcgcaagt
     7621 tggtaccaac gtaaaacatc tcaagattgg cgacacggtt gggctcggct ggttcagcgg
     7681 cagttgcctg acctgccatg cgtgcctgag tggggatcac aatttgtgcg cggatgccga
     7741 acagaccatc gtcggccgcc acggcggctt cgccgacaag gtgcgctgcc aggcgacgtg
     7801 ggccacgccg ctgcccgcag ggctcgacgt cataaaggcg gggccgctgt tctgcgccgg
     7861 catcaccgtg ttcaatcctc tcgtacagtt cgacgtgcgg ccgacgcagc gcgttggcgt
     7921 gatcggcatc ggcgggctcg gtcacctggc cttgcagttc ctcaacaagt ggggttgcga
     7981 ggtaacggcg ttcacatcca ccgacgccaa gcgcgaggaa gccctgcgga tgggtgcgca
     8041 tcacattgtc aactcacgcc acgcggcgca actgatgaag atcgccggtt cgctcgattt
     8101 cattctttcc acggtcgcgg tggaccttga ctgggacgct tatctcgacg cgctcgcgcc
     8161 caagggccga ctgcatatcg tgggagtcat tccctcaccc atggaagtga ccgctgatgt
     8221 attgatcggc ggtcagcata gtctctccgg ctcgccgacc ggtacgcccg caatgctgcg
     8281 caccatgctc gatttctgcg cgcgtcaccg catcgagccc gtgaccgaaa tcttccccat
     8341 gagccgtgtc aacgaagcta tcagccacct tcgcgccggc aaggcacgct accgtgtcgt
     8401 gttggagaac gattttggtt aacaggtaaa ttgaatattt tcgcggaaca cagcggttga
     8461 taataatgac cgggcggaat acgatcgccg ccgaagcagc aagcttgtcc tcttggcaag
     8521 tccccgaaat cgaaggtctc agaacaagat ctttctcaat caatctcgtt gcaaaggagt
     8581 atgcgtatgt ccatgttgaa aaagctgtcc attttgtccg tcggcctgat gatcgcggtg
     8641 ggacttgtcg cctgtgaaca aaaaggcccg gccgagaagg tcggcgaaaa gatcgaccag
     8701 actgtcgaaa aggccaagga aaaggttgag gatgtgacca agcccgaagg accgatggaa
     8761 aaggccggca agaagatcga ccaggccgtc gaggatgcca aagaggcaat caagaaataa
     8821 gctggcaagc gttaaataaa aaaggcgggt tcagaatgaa cccgcctttt ttacgacatc
     8881 accacaacag ttcccgttcc gaccgaaact ccctgtctgt acctgcgacg ggtccatgga
     8941 atcgaatcct cttgaccacg ggtcgtctcg gctagcggct ccatgtgacg cgcagcagat
     9001 cgccctcgtc tgtgtactgc gactccagct cgccatgata ggcgtggcgc agcgcctcgc
     9061 cgatcccgtg taccaggtgc gaatccgtaa aagtgaccac ggtgtgatcc gcttcatcct
     9121 tgatgtccat gatgcgttcg agcggatgct cggcgcgcgc tttcgactcg gcatggcgga
     9181 cgaggtgcag gatctcctcg cggtgctcgg cgaaaaactt cccgctcagt gtcagctcac
     9241 cggctggcac ccggtcatgt attcgctggc acgcgggaca gagatgctgc gcggccccgg
     9301 gcgccgccgc gccccaccgc cattgcccgt ga
//
LOCUS       DBNGZE010000027         1851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1184591, whole genome shotgun sequence.
ACCESSION   DBNGZE010000027 DBNGZE010000000
VERSION     DBNGZE010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1184591"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /gene="rsmG"
                     /locus_tag="AC16FV_00770"
     CDS             complement(<1..397)
                     /gene="rsmG"
                     /locus_tag="AC16FV_00770"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR2848627.1"
                     /translation="MKLEKRLQQGLREMGVDLPPAAVEKLLNFLQLLEKWNKTYNLTA
                     VRDPEQMVSRHLLDSLSVLPYLQGPRVLDIGTGAGLPGIPLALARPDLEFTLLDSNAK
                     KTRFAAQALHELGLKNVVVVQERVEKFHPA"
     gene            535..1011
                     /locus_tag="AC16FV_00775"
     CDS             535..1011
                     /locus_tag="AC16FV_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR2848628.1"
                     /translation="MKTFFLLFTAAILGATLPARAADEQAQADPSAKPAVLIPGVVTR
                     ALFTRDIENREPVDTVSTLTNNLTSIVYFTEIHDMAGQTVTHRWEYNGKIILEVPIKV
                     GTSRWRAYSTKTLNPSWVGEWKVSVVDAAGGTLSVNTFSYLKHPETPTSVATNPLP"
     gene            complement(1038..1673)
                     /locus_tag="AC16FV_00780"
     CDS             complement(1038..1673)
                     /locus_tag="AC16FV_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848629.1"
                     /translation="MQITPDSMLYYLVVLLIFGNVFALVIGVLMLAAPQQLSALFKFS
                     NRWISTRKLTKSLEKPRPTEPSMLRYPRVLGAILLASAALILIKGTIFITGMSAADGG
                     RLLARLYGDANISSSAWESLWLSLIAFIALGAVMAIAVGLMSLFKPGQLKHWGNSINR
                     WVSTRQLTKPLDTPHYHLDKLVVAQPRAWGGVITVLALFSAVVLWWFVLGA"
     gene            complement(1747..>1851)
                     /locus_tag="AC16FV_00785"
     CDS             complement(1747..>1851)
                     /locus_tag="AC16FV_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848630.1"
                     /translation="AAHRPATLGQAGRLSGMTPAAISLLLIHLRRRRA"
BASE COUNT          369 a          566 c          558 g          358 t
ORIGIN      
        1 ccgccggatg gaatttctcc acgcgctcct gcaccacgac tacatttttc aggcccagtt
       61 cgtgcagcgc ctgcgcggcg aagcgggttt tcttggcgtt gctgtcgagc agggtgaatt
      121 caagatcggg gcgcgccagc gccagcggaa tgccgggcag cccggcgccg gtgccaatat
      181 caagcacgcg cggcccttgc aggtatggca acaccgaaag actgtcgagc aggtgtcgcg
      241 acaccatctg ctcggggtcg cgcacggcgg tgaggttata ggtcttgttc catttctcga
      301 ggagttgcag gaaattcagg agcttctcca ccgccgcagg cggcagatcg actcccatct
      361 cgcgcaggcc ctgttgcagg cgtttttcga gtttcatggc cctgccgcat tccctttagt
      421 tgtttttgct cgcatttatc atgcaattta agcccttcgt ctcttttgcg ctatccaccc
      481 tccgggcctg tcctaagctc tttaaataca ggcctcaaat ggactttgta atgtatgaag
      541 acattttttc tgctttttac cgcggccatc cttggcgcaa ctctgccggc acgggctgcg
      601 gacgaacagg cgcaggctga tccgtcggcg aagccggcgg tgctgatccc cggcgtcgtg
      661 acgcgcgcgc tgtttacccg cgacatcgaa aaccgtgagc cggtcgatac ggtatcgacg
      721 ctcacgaata atctgaccag cattgtttac ttcacggaaa tccacgacat ggccgggcag
      781 acggtcacgc accggtggga atacaacggc aagatcatcc tggaagtgcc gatcaaggtg
      841 ggaacctcgc gctggcgcgc ctactcgacc aaaaccctca atccttcctg ggtcggtgaa
      901 tggaaggtct cggtggtgga tgccgccggc ggcaccctga gcgtcaatac cttcagctac
      961 ctgaaacatc ccgaaacacc cacttccgtg gcaacgaatc cgcttccctg agcgaaaaac
     1021 gaggtgcggc agatggctca agcgccgagc acgaaccacc acagcaccac ggcggaaaac
     1081 agcgccaata cggtgatcac gccaccccat gcgcggggct gggccaccac cagtttgtcc
     1141 agatgataat gcggggtgtc gagcggcttg gtcagctgac gcgtggacac ccagcggttg
     1201 atggagttgc cccagtgctt gagctggccg ggcttgaaca gcgacatcag ccccaccgcg
     1261 atcgccatca ccgcgcccaa cgcaatgaat gcgatcaagc tgagccacag gctctcccac
     1321 gcgctgcttg aaatattggc gtcaccgtag agacgcgcca acagcctgcc gccgtcggcg
     1381 gcactcatgc cggtgatgaa aatcgttccc ttgatcagga tcagcgccgc gctcgccagc
     1441 aggatcgcgc ccagcacgcg cgggtagcgc aacatgctgg gttcggtcgg gcgcggtttt
     1501 tccagcgact tggtcagctt gcgcgtggaa atccagcggt tggaaaattt gaaaagcgcg
     1561 ctcagttgct gcggcgccgc cagcatcagc acgccgatga cgagtgcgaa gacattgccg
     1621 aagatcagca gcacaaccag gtaatacaac atgctgtccg gggtgatttg catcgtgtcg
     1681 cctccagtgc gcggggttca cttgggtcaa cgtcccaatt gtagacgctg aaaatcgggg
     1741 gtgccgtcag gcgcggcggc ggcgcaaatg aatcagcagc agcgagatcg cggccggggt
     1801 catgccggag aggcgcccgg cctggccgag tgtggcgggc cggtgtgccg c
//
LOCUS       DBNGZE010000028         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_768175, whole genome shotgun sequence.
ACCESSION   DBNGZE010000028 DBNGZE010000000
VERSION     DBNGZE010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_768175"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="AC16FV_00790"
     CDS             complement(<1..381)
                     /locus_tag="AC16FV_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HIR2848631.1"
                     /translation="MTIDIQAVRDYLLNLQERICAALEKEDGKEKFSEDSWERPEGGG
                     GRSRVLAEGEVFEQAGVNFSHVHGKNLPPSATAHRPELAGRSFQALGVSLVIHPRNPY
                     VPTSHCNVRFFMAEKAGAEPVWWFG"
     gene            complement(492..1031)
                     /gene="tsaC"
                     /locus_tag="AC16FV_00795"
     CDS             complement(492..1031)
                     /gene="tsaC"
                     /locus_tag="AC16FV_00795"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HIR2848632.1"
                     /translation="MSGHLAHAARVIQQGGVIAYATEYCFGLGCDPMNRAAVLRLLRI
                     KRRPAQKGLILIAASVDQLAPYVDDIPPAVLATWPGPHTWLLQPRDGVPGWLTGDHPR
                     IAFRVTAHAQAAALCKAVGMAIVSTSANRAGGEPTRSFRDTVRRFQGEVDCVLPGRVG
                     DAPAPTPIRDAITGELVRA"
     gene            complement(1031..>1927)
                     /gene="purD"
                     /locus_tag="AC16FV_00800"
     CDS             complement(1031..>1927)
                     /gene="purD"
                     /locus_tag="AC16FV_00800"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014233273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR2848633.1"
                     /translation="EAYIRKVGAPIVVKADGLAAGKGVIIAQTVDEAITAVRDMLAGN
                     AFGEAGHRVVVEEFLTGEEASFIVMVDGAHILPLATSQDHKRVGDGDTGPNTGGMGAY
                     SPAPVVTPDIHARIMHEVIEPTVHGMKAEGHPYTGFLYAGVMIGADGTPKVLEYNCRF
                     GDPETQPVMLRLKSDFIELIEAAIDGKLDRIQAQWDSRAALGVVMAAGGYPGAYNKGD
                     VITGLPSSPAVDCMVFHAGTTLKGKDVITSGGRVLCVTALGATVSAAQKRAYEVASRI
                     RWSQAQYRHDIGYRAIARERAP"
BASE COUNT          347 a          620 c          599 g          361 t
ORIGIN      
        1 gccgaaccac cacaccggtt cagccccagc tttttccgcc atgaagaagc gtacgttgca
       61 atgcgaggtc ggcacataag gattgcgcgg gtggatgacg agcgaaacac ccagggcctg
      121 aaacgagcgc ccggccagtt ccggccggtg cgccgtggcc gagggcggca ggtttttccc
      181 gtgcacatgc gagaaattca cccccgcctg ttcgaacacc tcgccttcag cgagcaccct
      241 ggaacgcccg ccgccaccct ccggccgctc ccagctgtcc tcactaaatt tttccttccc
      301 atcctccttt tccaacgcag cgcaaattcg ctcctgcaag ttgagcagat agtcgcggac
      361 agcctggata tcgatggtca tgtaaaaatc gaatggacag gatttacagg attttaaaag
      421 gataaaactg gtttgttctt gcttttgttt taatcatgta aatcctgtta atcctgtcta
      481 ttgatgtttt gtcacgctcg cacgagttcg ccggtaatag cgtcgcggat cggcgtcggc
      541 gccggggcat cgcccactcg acccggtaaa acgcaatcca cctcgccctg gaagcggcgc
      601 acggtatcgc gaaaactgcg cgtcggttcg ccgccggcgc gattggcgct ggtggagacg
      661 atggccatgc caaccgcttt gcacaaggct gcggcctgcg cgtgcgcggt gacccggaag
      721 gcgatacgcg ggtgatcgcc ggtgagccag cccggcacgc catcgcgcgg ttgcagcagc
      781 caggtgtgcg gaccgggcca ggtggcgagc accgcgggcg ggatatcatc cacgtagggg
      841 gccagctggt ctacgctggc ggcgatgagt atcagacctt tctgcgccgg ccggcgcttg
      901 atgcgcagca gccgcagcac cgcggcgcgg ttcatggggt cgcagccgag gccgaaacaa
      961 tattcggtgg cgtaggcgat cacgccaccc tgctggatga cgcgcgccgc atgggccaag
     1021 tgaccggaca tcacggcgcc cgctcccgtg ctatggcgcg gtagccgatg tcatggcgat
     1081 actgcgcttg ggaccatcga atccggctgg caacctcata ggcgcgtttc tgcgccgcgc
     1141 tcacggtcgc accgagtgcg gtgacgcaca acacacgccc gccactggtg atcacgtcct
     1201 tgcccttcag tgtggtgccg gcgtgaaaaa ccatgcagtc gaccgccgga cttgagggca
     1261 ggccggtgat gacgtcaccc ttgttgtacg cgccggggta gccgccggcg gccatgacca
     1321 cgccgagcgc agcgcggctg tcccattgcg cctgaatccg atcgagtttg ccatcgatgg
     1381 cggcctcgat gagttcgatg aaatccgact tcaagcgcaa catcactggc tgggtttcgg
     1441 ggtcgccgaa gcgacagttg tattcgagca ccttgggcgt gccgtcggca ccgatcataa
     1501 cgccggcgta cagaaagccg gtgtaaggat gaccttcggc tttcatgccg tgcacggtcg
     1561 gctcgatcac ttcgtgcata atgcgcgcgt gaatgtcggg tgtgaccacc ggcgccggcg
     1621 aatacgcgcc catgccgccg gtattggggc cggtgtcacc gtcgccgaca cgcttgtggt
     1681 cctgcgaggt cgcgagcggc aagatgtgcg cgccgtccac catgacgatg aagctcgcct
     1741 cctcgccggt gagaaattcc tccaccacca cgcgatggcc ggcctcgccg aaggcgttgc
     1801 ccgccagcat gtcgcgcacg gcggtaatgg cttcgtccac cgtctgcgcg atgatcacgc
     1861 ccttgcccgc ggcgaggccg tcggccttga ccacgatcgg cgcgccaacc ttcctgatat
     1921 aagcctc
//
LOCUS       DBNGZE010000029         3941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1115415, whole genome shotgun sequence.
ACCESSION   DBNGZE010000029 DBNGZE010000000
VERSION     DBNGZE010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1115415"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="AC16FV_00805"
     CDS             complement(<1..691)
                     /locus_tag="AC16FV_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR2848634.1"
                     /translation="MTAHRKQIAVWLLIVAAMIFIMVVLGGTTRLTRSGLSIVEWRLF
                     QGTLPPLTEAHWRELFELYQQTPEYQKINVGMDLMGFKEIFWLEYLHRLWGRLIFFVA
                     LAPLLWFLWRGRIEKKLIPRMVAVPLLVALNGVLGWFMVASGLVDIPRVSPYRLTAHL
                     GLAVAIYAYILWMALNLVSERAPTSPPPGLRRFGHIVTVLVFLMILSGGFVAGTKAGF
                     AFNTFPLMNGRF"
     gene            complement(706..1704)
                     /locus_tag="AC16FV_00810"
     CDS             complement(706..1704)
                     /locus_tag="AC16FV_00810"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR2848635.1"
                     /translation="MTEHASNRQTSRNASGLYTLLVFVSILLVLIVVILSAWLRLSGS
                     GLGCAEWPSCYALIKTEISGVESLASAHLIPPWATLTHRLVASALGVLVLGIAVAAWR
                     RRKQPGQSVLIPFAVLGLTIFLSVLGYKTPSPLLPWVTLSNLLGGMAMLALLWWLGQR
                     SVSTQTEIATRHELRPWALAGIFVICFQIALGGWVSANFAALSCTDLPGCGGYPWRDG
                     PWRESFNLLRVLPATDAGAVITGDTGKIIHIVHRAGAALTLLYIGWLGWRAIASGSRY
                     RGTGITMLVFLVLQIFLGIGAVAFSLPLGLVTVHNAIAALLLLSVVNLYHLLTPRI"
     gene            2023..2190
                     /locus_tag="AC16FV_00815"
     CDS             2023..2190
                     /locus_tag="AC16FV_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848636.1"
                     /translation="MSAAKNMDSGRDEKVPAMQRVLDNPFLLLFLGVTMPTVFYILWG
                     IMEIVTLPLAK"
     gene            2209..2745
                     /locus_tag="AC16FV_00820"
     CDS             2209..2745
                     /locus_tag="AC16FV_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HIR2848637.1"
                     /translation="MALTPPAERLWWKEPIEAVELFWVAIALVWAIVMFVMMPYWHVY
                     GKQNLSNEAYRTTPEMYTQKVQAMVDQYTIRKETARDMPVVRPPAGADVYMLARLWEW
                     YPALELEKGQTYRLHLSSLDWLHGFSLQPENINMMVIPGYEMVLKITPNKAGEYSVIC
                     NEYCGIGHHQMIGRIYVK"
     gene            2758..>3941
                     /locus_tag="AC16FV_00825"
     CDS             2758..>3941
                     /locus_tag="AC16FV_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR2848638.1"
                     /translation="MANTAEFRTCPDTGLLFHKPAETLMKLNAVAGIVFLLIGGVLGL
                     LMGLTRWPTVHLLQADSFYMLLTAHGLSVLIFWIIFFEIAVLYFTSSTLLRSRLAGPR
                     WAWLGFVLMVVGSIMNMVAVFQGRSSVMMTSYVPMSAEPHFYLGLILFAVGALIGCFV
                     FLGTLVIARAERTYGEGSIPLVTFGALTACIIAIFTIASGAIILIPTFLWKVGYINHI
                     DPLMYRIVWWGMGHSSQQINVSAHIAVWYAVMGVVFGARPLSEKVSRGAFLLYILFLQ
                     LACAHHLLVDPAVGSEWKIFNTSYAMYLAVLASMVHGLTVPGAMEAAQRKRGFNKGLF
                     EWLRKAPWGNPTFAGVFLSILIFGFLGGISGVVMGTEQINLIIHNTLYVPGHFHATVV
                     GGT"
BASE COUNT          821 a         1151 c         1173 g          796 t
ORIGIN      
        1 aaaaccggcc attcatcagc gggaaagtgt tgaaggcaaa ccccgccttg gtgcccgcga
       61 cgaaaccgcc cgagaggatc atgagaaaca ccagcacagt gacgatgtga ccgaaacgcc
      121 gcaaccccgg cggcgggctg gtcggtgcgc gttcggagac aaggttaagc gccatccaca
      181 gaatgtatgc gtagatcgcc accgccagcc ccaggtgcgc cgtcaggcgg taggggctta
      241 cgcgcgggat gtcgacaaga ccgctggcca ccatgaacca gccgagcacg ccattcagcg
      301 ccaccagcag cggcaccgcc accatgcgcg ggatcagttt cttctcgatg cggccgcgcc
      361 acaggaacca tagcaacggc gccagcgcga cgaagaaaat cagccggccc cagagacggt
      421 gcaggtattc gagccagaat atttccttga agcccatgag atccatgccg acgttgattt
      481 tttgatattc gggcgtttgc tggtacagct cgaacagctc ccgccagtgc gcctcggtca
      541 gcggaggtaa cgtgccttgg aacagccgcc attcgactat ggacagcccc gagcgtgtca
      601 gccgcgtggt cccgcccagc accaccatga tgaaaatcat ggctgcgacg atcagcagcc
      661 agacagcgat ctgttttcga tgtgcagtca tataagcaga acttcctaaa ttcgtggcgt
      721 caggaggtga tacaggttca cgacggacag caataatagt gcggcaatcg cattatgcac
      781 cgtgaccaga ccgaggggca ggctgaaggc gacggcgccg atgcccagga atatttgcag
      841 tacgagaaaa accagcatcg tgatgcccgt gccgcgatag cggctgcccg atgcgatggc
      901 gcgccatccc agccatccta tatataagag tgttaacgct gcccccgcgc gatgcacgat
      961 atggatgatc ttgccggtat cacccgtgat aacggcgccg gcatccgtgg cgggcaatac
     1021 gcgcaacagg ttgaagctct cgcgccaggg accgtcgcgc cagggataac cgccgcaacc
     1081 tggcagatcg gtgcaggaca gcgccgcgaa attggcgctg acccatccgc cgagtgcaat
     1141 ctgaaagcag ataacgaaaa ttccagcgag cgcccatggc cgcaattcgt gtcttgttgc
     1201 gatttctgtt tgtgttgata cggaacgctg ccccagccac cacagcaagg ccagcatcgc
     1261 catgccgccg agcagattgc tgagcgtgac ccacggcagc aacggcgaag gcgttttata
     1321 acccagcacc gaaagaaata tggtcagtcc cagcaccgcg aatggaatca ggacggattg
     1381 tcccggctgt ttacgacgac gccaggccgc gaccgcgatc cctaatacca acacccccag
     1441 cgcggaggcg accaggcggt gtgtcagggt ggcccacggc ggtatcagat gcgcagaggc
     1501 aagcgattcc acgccgctta tttccgtttt gatcagcgcg tagcacgacg gccattcggc
     1561 acaacccaga ccgctgcccg agagacgcag ccaggcgctc agtatcacca caatcagcac
     1621 cagcaagatg gagacaaaga ccagcagcgt ataaaggccg ctcgcgtttc tggatgtctg
     1681 acggttggag gcgtgttcgg tcatggaccg atcaggttag gcgaggccgg gaatggctgc
     1741 aagcaccgat tatttttttg tcgcttgatg ttgatcatgg cgaaatagca ggcgcctgct
     1801 actttttttg gcgacgcaat cgcatgcgcg tttgatcggc gtcaaagttt aaaacaattt
     1861 cggacaaatt gatctaaatc aagtttctac acgccccaaa cggttttaaa ttcgtagcgg
     1921 cattccgtcg cagcatggaa attgaattaa tggagccggc gttgcgctca gcggcccggc
     1981 aaaaacggcc aacggtattt cgatcctatc tggaggtgtg acatgtcagc agccaaaaac
     2041 atggattcag gccgggatga aaaggttccg gcgatgcagc gggtgctcga taacccgttt
     2101 ctgctgttgt tcctcggcgt gaccatgccc acagtgtttt acatcctgtg gggcatcatg
     2161 gaaatcgtca ctttaccctt agccaagtaa ctttcgggag acgctcacat ggccctaacc
     2221 cctcctgccg aacgcctgtg gtggaaagaa ccgatcgaag ctgtcgagct gttctgggtc
     2281 gccatcgcgc ttgtctgggc gatcgtgatg ttcgtcatga tgccctactg gcacgtttac
     2341 ggtaagcaga acctttccaa cgaggcctat cgcaccacgc ccgagatgta cacccagaaa
     2401 gtccaggcga tggtggacca atacacgatc cgcaaggaaa ccgcgcgtga catgccggtg
     2461 gtgcgtccgc ccgcgggagc cgatgtctac atgctggcgc gcctgtggga atggtatccg
     2521 gcgctggagc tcgaaaaggg ccagacctac cgtctgcacc tgtcttcgct ggactggctg
     2581 catgggttct cgctgcaacc ggagaacatc aacatgatgg tgatcccggg ttacgaaatg
     2641 gtgctgaaga tcacgccgaa caaagcggga gagtactcgg tgatctgcaa cgaatactgc
     2701 ggcatcggtc atcaccagat gatcggcaga atctacgtga agtaggagga gaggcccatg
     2761 gcgaatactg cggaattccg tacctgtccc gacaccggtc tgctgttcca caagccggcc
     2821 gagacgctga tgaaactgaa cgccgttgcc ggcatcgtgt tcctgttgat tggcggcgtg
     2881 ctcggcctgc tgatgggctt gacgcgctgg cccaccgtgc acctgctgca ggccgattcg
     2941 ttctacatgc tgctgaccgc gcacggcctc agcgtgctga tcttctggat catcttcttc
     3001 gagattgcgg tgctgtactt cacctccagc accctgctgc gctcccgtct cgcgggaccg
     3061 cgctgggctt ggctcggctt cgtgctcatg gtggtgggct cgatcatgaa catggttgcc
     3121 gtcttccagg ggcgttcgag cgtgatgatg acctcgtatg tgccgatgtc ggcggaaccg
     3181 catttctacc tgggcctgat cctgttcgcg gtcggcgccc tgatcggctg ttttgtgttc
     3241 ctgggtacac tggtgatcgc gcgcgcggag cgcacctatg gcgagggttc gattccgctg
     3301 gtgaccttcg gcgcgctgac ggcgtgcatc attgccatct tcaccattgc ctcgggcgcc
     3361 atcatcctga ttcccacctt cctgtggaag gtcggttata tcaatcatat cgatccgctg
     3421 atgtaccgca tcgtctggtg gggcatgggc cattcctcgc aacagatcaa cgtttcggcg
     3481 catatcgcgg tgtggtacgc cgtgatgggc gtggtgttcg gcgcgcggcc gctgtcggag
     3541 aaggtctcgc gcggggcgtt cctgctgtac atcctgttcc tgcaactggc ctgcgcacac
     3601 cacctgctgg tcgacccggc cgtcggttcg gaatggaaga tcttcaatac cagctacgcc
     3661 atgtacctgg cggtgctggc gagcatggtc cacggcctga cggtgccggg tgcgatggaa
     3721 gcagcgcagc gcaagcgcgg tttcaacaag ggcctgttcg aatggctgcg caaggcgccg
     3781 tggggcaacc cgacgttcgc cggtgtattt ttgtcgatcc tgatattcgg tttcctcggc
     3841 ggaatttccg gcgtggttat gggcacggag cagatcaacc tgatcatcca caacaccctg
     3901 tacgtgccgg gacatttcca cgcgaccgtg gtgggcggca c
//
LOCUS       DBNGZE010000030         2120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1324147, whole genome shotgun sequence.
ACCESSION   DBNGZE010000030 DBNGZE010000000
VERSION     DBNGZE010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1324147"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16FV_00830"
     CDS             complement(<1..448)
                     /locus_tag="AC16FV_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848639.1"
                     /translation="MDKKLWTPVLLPVLLTAYPACADIFQDQITKSFRGFVIMDYSEF
                     DPDIRKNLDANPAFITGDFNYDKFEDFAALIRDTKKKHYIAGESSYDYYETKLVACHG
                     LGNRKYNCTALFSGVTVLPEFHYLVKHPPGKTKCRSPQGDSVEAETE"
     gene            441..689
                     /locus_tag="AC16FV_00835"
     CDS             441..689
                     /locus_tag="AC16FV_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848640.1"
                     /translation="MSIITSPCGHDRDSQNTPAVDVTHVIAWRHFHNTWEFVRQEAEK
                     TELPYRITWYFLTPANPRSYLKNVASGRQPGEGEAEIS"
     gene            complement(810..1619)
                     /gene="tatC"
                     /locus_tag="AC16FV_00840"
     CDS             complement(810..1619)
                     /gene="tatC"
                     /locus_tag="AC16FV_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR2848641.1"
                     /translation="MAEPEKPAAAEGSLISHLLELRDRLLYAVIAIAVVFFALIPFAG
                     DVYTLVAKPLMSVMPPGATLVATEVASPVLTPSKLTLAVAIVITIPVLLYQLWAFVAP
                     GLYKHERRLVFPLLLSSTILFYGGMAFAYFVVFPMAFGFFVHALPPGVTMMTDIRSYM
                     DFVFSMFFAFGIAFEVPIAVILLARMGVINPETLAQKRPYVILWAFIVAAILTPPDIF
                     SQFFLAVPMIILFEIGLFVARLMKRAADNAVNGGRRDLTEDEIEAEFKKND"
     gene            complement(1588..2025)
                     /gene="tatB"
                     /locus_tag="AC16FV_00845"
     CDS             complement(1588..2025)
                     /gene="tatB"
                     /locus_tag="AC16FV_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR2848642.1"
                     /translation="MFDIGFSELVAIGLIALIVLGPKRLPEVARTAGRWVGQLRRFIA
                     DVKQDIDREMHTEDLAELRKLRQELNDTRRLTENSSGELLKGFAEMTPESATPTIHSP
                     EPPQLPSGDSATTSTPAWRKRAKSRNKSRPGKKHGGTRKARRR"
BASE COUNT          445 a          602 c          639 g          434 t
ORIGIN      
        1 attcggtctc ggcttcgaca gaatcgccct ggggtgaacg gcatttcgtc tttcccggtg
       61 gatgctttac caggtaatga aattccggca gtaccgtgac accactgaat aatgccgtgc
      121 agttgtattt cctgttgccc aggccgtgac acgccaccag cttggtttcg taataatcgt
      181 aggagctttc gccggcgata tagtgctttt ttttggtatc gcgtattaac gcagcaaagt
      241 cttcgaattt gtcgtaattg aaatctccgg tgataaaggc gggattggca tccagatttt
      301 tccggatgtc cggatcgaac tcgctataat ccatgatcac gaaaccgcga aaggatttgg
      361 tgatctggtc ctggaaaata tccgcgcagg ctgggtaagc cgtcagcaag accggcagca
      421 aaaccggcgt ccacaatttc ttgtccatta ttacttctcc ctgcggacac gaccgtgatt
      481 cccagaatac acctgctgtg gatgtcacgc atgtcattgc ctggcggcat ttccacaaca
      541 catgggaatt cgtcaggcag gaggcagaaa aaacggaatt gccttaccgc attacgtggt
      601 attttttaac gccggcaaat cccagaagct atctcaaaaa cgtcgcgagc ggccgtcagc
      661 cgggtgaggg tgaggcggag atttcatgaa cgtccgatgg acgttcatgc cgccgaacgg
      721 gccgccaagg atggcggccc cggactttcc cgcggagccg gatagcgcag cgggaaagga
      781 gcgcagcgca gcaggttttg agatagcttc tagtcgttct tcttgaactc ggcctcgatc
      841 tcatcctcgg tcaggtcgcg ccgtccaccg ttcacggcgt tgtcggcggc gcgtttcatc
      901 aagcgcgcga cgaaaagccc gatctcgaac aatatgatca tcggtacggc gaggaaaaac
      961 tgcgaaaaaa tatccggtgg cgtgaggatg gcggcgacaa tgaaggccca caggatcacg
     1021 tacggccgtt tctgggccag cgtctcggga ttgatcacgc ccatgcgcgc cagcaggatc
     1081 accgcgatcg gcacctcgaa ggcgatgccg aaggcgaaga acatggaaaa cacgaagtcc
     1141 atgtaggaac ggatgtccgt catcatggtc acgcccggcg gcagggcgtg cacgaaaaat
     1201 ccgaaggcca tcgggaacac gacgaaatag gcgaatgcca tgccgccgta aaacagaatg
     1261 gtgctcgaca acagcagcgg aaacaccagc cgccgctcat gcttgtacag gcccggcgcc
     1321 acgaaggccc acagctggta tagcagaacc ggaatcgtga tcacgatcgc caccgccagt
     1381 gtcagcttgc tgggcgtgag gaccggcgag gccacctcgg tcgcgaccag ggtcgcgccg
     1441 ggcggcatca cgctcatcag cggtttcgcc accaatgtat aaacgtcccc cgcgaaggga
     1501 atcagcgcga agaacaccac ggcgatggcg atgaccgcgt agagcaggcg atcgcgcagc
     1561 tccagcaggt gggaaatcag gctgccttca gcggcggcgg gcttttcggg ttccgccatg
     1621 tttttttccg ggccgggatt tatttctgga tttggcgcgc ttgcgccacg ccggcgtgga
     1681 cgttgtcgcg ctgtctccgg aggggagttg aggcggctcc ggcgaatgaa tcgtgggcgt
     1741 ggcggattcg ggcgtcattt ccgcgaaccc cttgaggagt tcgcccgagc tgttttccgt
     1801 caggcggcgg gtgtcgttca gctcctgcct gagcttgcgc agctccgcca ggtcctcggt
     1861 atgcatctcg cggtcgatgt cctgcttcac gtccgcgatg aagcgccgca actgtcctac
     1921 ccagcggccg gccgtgcgcg ccacttccgg caggcgtttc ggacccagca cgatcagcgc
     1981 aatcaggccg atcgccacca gttccgaaaa gccgatgtcg aacatcgcgc gtggccgtga
     2041 cgggagacgg gcttaggtct tgtgtttctg cttggaggtg acttccgcgt cgattacacg
     2101 gccggaatcg ttcttgatct
//
LOCUS       DBNGZE010000031         2818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_73943, whole genome shotgun sequence.
ACCESSION   DBNGZE010000031 DBNGZE010000000
VERSION     DBNGZE010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_73943"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="AC16FV_00850"
     CDS             complement(<1..1747)
                     /locus_tag="AC16FV_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848643.1"
                     /translation="METSDPKYKHKPSEKHTLEEVLKSLQDLIRNDLAEGAAAAGKQE
                     PAPAEPRQHEDTARGMTEAKPRHENTAGLREDFAPVSPGSGPVNLDAVMRSLKDLIGN
                     ELNVGDEPKPPESKSVVHEEYLAAEEKIEEYIPDELGQLEDELNIGEPAAPEQAASES
                     PHDEPFAPEGIIEEITPERFTPLDEELTLEELAEVAPLTPDAAEPTPPRDESPAHEEI
                     VEASLPEEFVPLDEELTFEKPPEIASPAPVLPETPELPGEISLELLGEPETTPPAVTE
                     TPPAMKEDIAPGTQHEIFFEEPPTPSGEPQAAPTDSVSASEPELAPQAIQEPVADVSQ
                     SPAEETALPTIEVEESFDTGNYFDGAALSAENSPAALEEIILQDMEISNAPGTTPPAV
                     ETSPAAPTDVVPAPGSPPAPVEKKVTLEIVEEPTSDGSPKASVDFDALGLEPSQPEQA
                     PPPVVADIPAPEAESPVTTAKPDATPAPKPKPPKAVEKTPTVETKAETAPRPAQPPAP
                     AEPGTTKTPPTLNLDDIPVLNEVVAPPAGSAPVAKPDAPPSTLPAPDRARDIVVRAVA
                     KLNVEMRKTGGAGLDT"
     gene            complement(1751..2200)
                     /locus_tag="AC16FV_00855"
     CDS             complement(1751..2200)
                     /locus_tag="AC16FV_00855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR2848644.1"
                     /translation="MTRVDFYLIDNATDGSQDAAVCKLTHKAFRLGHRVYILTPDFSH
                     AQRLDRLLWIFSAGSFIPHGLSSETVDTDMPVLIGYDEPPAAYEDVLIQLAPQVPECF
                     SRFQRVAEVVSGADEDKARARERFRFYRDRGYALQTHNISAGDFVEA"
     gene            complement(2197..>2818)
                     /locus_tag="AC16FV_00860"
     CDS             complement(2197..>2818)
                     /locus_tag="AC16FV_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR2848645.1"
                     /translation="LGAIKAAAELKLPLNIVGIVPISENLPDGDANKPGDIVTSMSGQ
                     TVEVLNTDAEGRLILSDALTYAERYKPAVVIDIATLTGACVIALGAHASGLLANNDQL
                     ARDILSAGKYTYDRAWQLPLWDEYQKQLDSNFADMANIGGREAGTITGACFLARYAKN
                     FKWAHLDIAGTAWKTGKEKGATGRPVPLLVQFLLNRAHKGDAETAA"
BASE COUNT          424 a          816 c          926 g          652 t
ORIGIN      
        1 tggtgtcgag gccggctccg ccggttttgc gcatctcgac attgagcttg gctaccgcac
       61 gcacgacgat gtcacgggcg cggtccggcg ctggcagcgt ggagggcggc gcgtcgggct
      121 ttgccaccgg tgcgcttcct gcgggcggcg ccacgacttc attcaatacc gggatgtcat
      181 cgagattgag cgtgggcggc gttttcgttg tgccgggttc cgccggagcc ggtggctgtg
      241 ctggccgagg cgccgtttcg gccttggttt caacggtggg cgtcttttcc acggcttttg
      301 gaggtttcgg cttcggggcc ggggttgcgt caggtttggc ggttgtcacc ggcgattcag
      361 cttccggcgc cgggatgtca gcgacaactg gaggtggagc ttgttccggc tgtgaaggtt
      421 ccaaaccgag cgcgtcgaag tccaccgatg ctttcgggga gccatcgctc gtcggttctt
      481 cgacaatttc gagggtaacc tttttctcta ctggcgcagg tggcgaccct ggtgcgggaa
      541 cgacatccgt cggtgccgct ggagatgtct cgacggctgg gggagtcgtt cccggcgcgt
      601 tggagatttc catatcctga agaatgatct cttccagcgc tgcgggagaa ttttccgccg
      661 acagcgccgc cccgtcgaaa taattgcccg tatcgaagct ttcttcgact tcgatggttg
      721 gcagcgcggt ttcttcagca ggagattgtg acacatccgc gaccggctcc tgtattgctt
      781 gcggcgccag ttcaggctca gacgcggaga ctgaatctgt tggcgcagct tgaggttccc
      841 cggaaggcgt cggcggttct tcgaagaaga tctcatgttg cgtgcccggt gcgatgtctt
      901 ctttcatcgc gggtggagtt tcggtaacag ccggtggtgt ggtttccggt tcgccgagca
      961 gctccagtga aatctcgccg gggagttccg gcgtttctgg caggaccggc gcgggcgatg
     1021 cgatctccgg cggtttttcg aacgtcaact cttcatcgag cggaacaaat tcctcgggca
     1081 ggctcgcttc aacaatctct tcatgtgcgg gcgattcatc gcgcggtggc gtgggttcag
     1141 ctgcgtccgg cgtgagcggc gcaacttcag ccaattcttc aagtgttaat tcttcatcca
     1201 gcggcgtgaa tctttccggg gtaatttctt caataatccc ttcaggtgca aacggctcgt
     1261 catgcggtga ttcagacgcg gcttgttccg gcgcggctgg ctcgccaata ttgagttcat
     1321 cctcaagctg gcccagctcg tccggaatat actcctcgat tttttcctcc gcggcgagat
     1381 attcctcgtg caccaccgat tttgattccg gcggttttgg ttcgtcgccg acgttgagct
     1441 cattgccaat gaggtccttc aaggatcgca ttacggcatc gagattgact ggccccgagc
     1501 caggcgacac cggcgcaaaa tcttcccgca ggccggctgt attctcgtgg cggggcttgg
     1561 cttcggtcat tccccgggcc gtgtcctcgt gctggcgcgg ttctgccggt gccggttcct
     1621 gtttgccggc cgccgcggca ccttcagcaa ggtcgttgcg gatcaggtcc tgcagcgatt
     1681 tcagcacctc ttcaagcgtg tgcttctccg agggcttgtg cttgtatttg ggatcggacg
     1741 tctccatgac tcaggcctcg acaaaatcgc cggcggagat gttgtgcgtt tgcagcgcgt
     1801 agccgcggtc gcggtaaaaa cggaaacgct cgcgcgcccg cgccttgtcc tcgtcggcgc
     1861 cgctgaccac ctcggctacg cgctggaagc ggctgaaaca ctccggtacc tgcggcgcca
     1921 gctgaatcag cacatcctcg taggctgccg gcggttcatc atagccgatc aggaccggca
     1981 tgtcggtatc cacggtttcc gagcttaatc cgtgggggat gaaactgccg gcgctgaaaa
     2041 tccacagcag ccggtcgagc cgctgtgcgt ggctgaaatc gggggtcagg atataaacac
     2101 gatggccgag gcgaaaggcc ttgtgggtca gtttgcagac agcggcgtcc tggctgccgt
     2161 cggtggcatt gtcgataaga tagaaatcga cgcgcgtcat gccgcggttt ccgcatcgcc
     2221 cttgtgcgcg cggttcagca ggaattgcac cagcagcggc actggccggc cggtcgcgcc
     2281 cttttccttg ccggttttcc aggcggtgcc ggcgatgtcg aggtgcgccc atttgaagtt
     2341 tttcgcgtaa cgcgcgagga agcacgcgcc ggtgatggtg ccggcctcgc gtccgccgat
     2401 gttggccatg tcggcgaaat tgctgtcgag ctgcttctgg tattcgtccc acagcggcag
     2461 ttgccaggcg cggtcgtagg tgtacttgcc ggcgctgagg atatcgcgtg ccagctgatc
     2521 gttgttggcc agcagcccgc tggcatgcgc gccgagcgcg atcacgcagg cgccggtgag
     2581 cgtggcgatg tcgatcacca ccgccggctt gtagcgttcg gcgtaggtga gcgcgtcgga
     2641 caggatcagc cggccctcgg cgtcggtgtt cagcacttcc accgtctgac cggacatgct
     2701 ggtgacgatg tcgccgggtt tgttggcgtc gccgtcggga agattctccg agatcgggac
     2761 gatgccgacg atattgagcg gcagtttcag ttcggccgcg gccttgatcg cgccgagc
//
LOCUS       DBNGZE010000032         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1741113, whole genome shotgun sequence.
ACCESSION   DBNGZE010000032 DBNGZE010000000
VERSION     DBNGZE010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1741113"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="AC16FV_00865"
     CDS             <1..121
                     /locus_tag="AC16FV_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase delta/epsilon chain alpha-helix
                     domain-containing protein"
                     /protein_id="HIR2848646.1"
                     /translation="EEALKSRQANIDYAKAQAELAEAIAQLRAIQKLRERTRK"
     gene            169..654
                     /locus_tag="AC16FV_00870"
     CDS             169..654
                     /locus_tag="AC16FV_00870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR2848647.1"
                     /translation="MTAVVAKNKVVSLSYVLRNQKGDIFEIRDLPVSYVHGSGTDLFP
                     KIEQALEGHAVGERVSVQLTPADAFGDHDPKLTFTDDIENAPPELRRVGAEFEAQNAK
                     GESIVLTVTRIEGDKITVDANHPLAGQTISFEVTVQDIRDATPEEIRNGRPAGDLPTL
                     Q"
     gene            693..2060
                     /gene="glmU"
                     /locus_tag="AC16FV_00875"
     CDS             693..2060
                     /gene="glmU"
                     /locus_tag="AC16FV_00875"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR2848648.1"
                     /translation="MPAPLEIVILAAGQGKRMYSDLPKVLHGLAGKPLLGHVLDTARA
                     LRPSAIHVVYGHGGEQVRAAFPHSELIWVHQAEQKGTGHAVAQAMPRVADQATVLVLY
                     GDVPLIRAQTLQELLTAAGNGLALLTAELDNPAGYGRILRDPAGQVARIVESKDASPE
                     EARVREVNTGFLAAPAGKLRKWLASLKNHNVQGEYYLTDIISMAVAEKLAIATRAPRD
                     IAEILGVNSKPELAQLERMYQKQQAEKLMQRGVTLRDPARLDVRGELTCGRDVVIDVN
                     VIFEGKVTLGDRVQIGPNNVIRDSTVGSGTIIQPNCVIEQADIGADCRIGPFARLRPE
                     TKLAAHVHVGNFVEVKKSEVGEGSKMNHLSYIGDTTIGSGVNIGAGTITCNYDGANKH
                     RTIIGDHVFVGSNTALVAPVTVGTGATIGAGSVITKEAPAGELTLTRAPQVTKPGWTR
                     PVKKK"
BASE COUNT          471 a          734 c          685 g          380 t
ORIGIN      
        1 cgaggaggcg ctcaagagcc gccaggcgaa tatcgactac gccaaggccc aggccgagct
       61 ggccgaggcc atcgcgcagc tgcgcgccat ccagaaactg cgcgaacgca cccgcaagta
      121 agcggcgtcc gtttatctca ttttcattta ttttttcatt ttccgttcat gaccgccgtc
      181 gtcgccaaaa acaaagtcgt ttccctcagc tacgtgttgc gcaaccagaa gggagacatc
      241 ttcgagatcc gcgacctgcc ggtgtcctac gtgcacggct ccggcaccga tctgtttccc
      301 aagatcgaac aggccctgga agggcacgcg gtgggcgagc gcgtctcggt gcagctgacc
      361 ccggccgacg ccttcggcga ccacgacccc aagctcacct tcaccgacga catcgagaac
      421 gcgccgcccg agcttaggcg cgtcggcgcc gagttcgagg cgcagaacgc caagggcgag
      481 agcatcgtgt tgacggtgac gcgcatcgag ggcgacaaga tcacggtcga cgccaaccac
      541 ccgctggccg gccagaccat cagcttcgag gtgacggtgc aggacatccg cgacgccacc
      601 cccgaggaaa tccgcaacgg ccgccccgcc ggtgatctgc cgacgctgca ataaatcgtc
      661 ccgccgggcg gttttttcgt atagttaaca gcatgcccgc ccccctcgaa atcgtcatcc
      721 tggccgccgg ccagggcaag cgcatgtact ccgacctgcc caaggtgttg cacggcctgg
      781 ccggcaaacc gctgctgggg catgtgctcg acactgcgcg cgccctgagg ccttccgcga
      841 ttcacgtcgt ctacggccac ggcggcgaac aggtgcgcgc ggcttttcca cattctgagc
      901 tcatctgggt gcatcaggcg gaacagaaag gcaccggcca tgccgtggcc caggccatgc
      961 ccagggtcgc cgatcaggcc accgtgctgg tgctctacgg tgatgtcccc ctgatccgcg
     1021 cccagacgct ccaggaattg ctcacggccg cgggtaacgg cctggccctg ctcacggcgg
     1081 aattggacaa ccccgcgggc tatggccgca tcctgcgcga cccggccggc caggtggccc
     1141 ggatcgtgga aagcaaggac gcgagccccg aagaggccag ggtgcgcgag gtcaataccg
     1201 gttttctcgc cgcccccgcg ggaaagctca ggaaatggct ggccagcctc aagaatcaca
     1261 acgtccaggg cgagtattat ctgactgata taatttccat ggcggtggcg gaaaagctgg
     1321 cgatcgccac ccgcgccccc cgcgatatcg ccgaaatcct cggggtcaac agcaaaccgg
     1381 agctcgcgca gttggagcgc atgtaccaga aacagcaggc ggaaaagctc atgcagcggg
     1441 gtgtaaccct gcgcgatccc gcgcgcctcg acgtgcgcgg cgagctgacc tgcgggcgcg
     1501 acgtggtgat cgacgtgaat gtcattttcg aaggcaaggt cacgctcggt gaccgcgtgc
     1561 agatcggccc gaacaacgtc atccgcgaca gcaccgtggg cagcggcacc atcatacagc
     1621 cgaactgcgt cattgagcag gccgacatcg gcgccgactg ccgcatcggc ccctttgccc
     1681 gattgcgccc ggaaacgaaa ctcgcggcgc atgtgcatgt cggcaatttc gtcgaggtga
     1741 aaaaatccga ggtcggcgaa ggcagcaaga tgaatcacct gagctatatc ggcgacacca
     1801 ccatcggcag cggcgtcaac attggcgccg gcaccatcac ctgtaattac gacggcgcca
     1861 acaaacaccg taccatcatc ggagaccacg tcttcgtcgg atcgaacacg gcgctggtgg
     1921 ctccggtgac cgtgggcacg ggcgccacca tcggcgccgg ttcggtgatc acgaaagagg
     1981 cgccggccgg cgagctgacc ctgacgcgcg cgccgcaggt aaccaagccc ggctggacac
     2041 ggccggtgaa gaaaaaatag cgtcaagtgt tgagtggtga gtgttaagtt ttcgacttgc
     2101 caaccttgcc tatcagcact aaatagttaa tttattactt aatacttatc actcaacact
     2161 aaaaactgat ttcaatatgt gtggcattgt cggcgcggtc gcgcagcgta acgtggttcc
     2221 cattctggta gaggggctga agcgcctcga ataccggggc tatgactccg
//
LOCUS       DBNGZE010000033         1999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_144371, whole genome shotgun sequence.
ACCESSION   DBNGZE010000033 DBNGZE010000000
VERSION     DBNGZE010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_144371"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16FV_00880"
     CDS             <1..375
                     /locus_tag="AC16FV_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2848649.1"
                     /translation="VFLFSVFPVLYNTVEGARSIKPELIEVAKSYRSSEWSLWREVML
                     PYTLPYMMTGVRQAIGRALVGMIAAEFFLSSTGLGQLIMGASQDFDTAGVFASILVIG
                     LIGVGLTRLGLKIEQHFARWRQ"
     gene            379..1212
                     /locus_tag="AC16FV_00885"
     CDS             379..1212
                     /locus_tag="AC16FV_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2848650.1"
                     /translation="MTETTIGETSAAISGTSASSRPVALLQGSFLPRFVTGLVIILSW
                     EFVVRALAPAYVAKPTTVVLAIPHVITDPAFLQALGATLAAVAEGLAITIVVGTAIGL
                     LMGRSNTADQMIRVYINGFNAMPMIIVLPLFSLWFGYSAAARIATIIFAAIFAITMNV
                     ADGARSVPREYLEVARSFRSGRLRMLTEIVLPASMPYLLAGFRLAAGRALIGAVVAEF
                     FLSVGGLGYYILYNSRSYHHNEAFVGVLLLAAFGVGFELLVVWATRRFMPWYRRDEKA
                     S"
     gene            1249..>1999
                     /locus_tag="AC16FV_00890"
     CDS             1249..>1999
                     /locus_tag="AC16FV_00890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR2848651.1"
                     /translation="MSSDPLLKAAITHWGPRFVANGVVLTDFEEVTGSISSYNDWCSA
                     WSARAAHHEQLGREALTKKHFLTAGECLQRAGVYYHFASFLFVHDQAQMKAAHKKQIE
                     CRQAALPHLRPPGERVEIPYQGKTLAGILRKPGGIERPPVVIMSVGLDSTKEEGDAYE
                     APFLARGMATLIFEGPGQGEAQYDFAIRGDYEVPVKAVIDFVEKRSDLDAKRVGRWGV
                     SLGGYYAPRAAAFDKRIKASIALGGPFNWGAA"
BASE COUNT          343 a          595 c          616 g          445 t
ORIGIN      
        1 gtctttctat tctccgtgtt tccggtgctt tacaacacgg tggaaggcgc gcgcagcatc
       61 aagcccgagt tgatcgaggt ggcgaaatcc taccgctcca gcgaatggtc gttgtggcgc
      121 gaggtgatgc taccttacac cctgccttac atgatgaccg gcgttcgcca ggcgatcggg
      181 cgcgcattgg tcggcatgat cgcggcggag ttcttcctgt cctcgaccgg cctcggtcaa
      241 ctcattatgg gcgcctcaca ggatttcgat actgcgggcg tgttcgcttc catcctagtg
      301 atcggcttga tcggcgtcgg attgacgcga ctcggcttga agatcgagca gcatttcgcg
      361 cgctggagac agtaggtgat gaccgagacg accatcggtg aaacatccgc cgcgatctcc
      421 gggacgtcgg catcgtcacg tcctgtcgcg ttgctgcagg gaagtttcct accgcggttc
      481 gtcacggggc tggtgatcat cttgagttgg gagttcgtag tccgcgctct cgctcctgct
      541 tacgtggcaa agccgacgac cgtcgtgctc gctattccac acgtaataac agacccggca
      601 tttctgcagg cgctcggcgc tacgcttgcg gcggttgccg aagggttggc gattacgatc
      661 gttgtcggta ccgccattgg gctgctgatg ggacgcagca acaccgccga tcagatgatc
      721 cgcgtctaca tcaatggttt taacgccatg ccaatgatca tcgtgttgcc gctgttctcg
      781 ttgtggttcg gctattcagc ggctgcacgc attgccacca ttatcttcgc tgcaatattc
      841 gcgatcacga tgaatgtcgc cgatggcgcc cgctcagtgc cacgcgaata cctggaagtt
      901 gcacgctcgt tccgttccgg ccgtctgcgg atgttgaccg aaatcgtctt gcccgcgtcg
      961 atgccttatc tcctcgcagg ctttcggctg gctgccggcc gcgcattgat cggcgcagtg
     1021 gtggccgaat ttttcctctc ggtcggcggg ctcggttatt acatcctcta caactcgcgc
     1081 tcttatcatc ataacgaagc cttcgtcggc gtgctgctgc tcgcggcatt cggagtcggt
     1141 ttcgaattgc tcgtggtttg ggcgacgagg cgtttcatgc cgtggtaccg ccgcgacgaa
     1201 aaggcgagct agcgtgcgcg tcctgcgact atcataagga aatctcgtat gtcgtctgac
     1261 cccctgctca aggccgcgat cacgcattgg ggcccgcgct tcgttgccaa tggtgtggtg
     1321 ctaaccgact tcgaggaggt caccggctcg atctcttcat ataatgactg gtgcagcgcc
     1381 tggtccgcgc gcgcggcgca ccacgagcag ctcgggcggg aggccctcac aaagaagcat
     1441 ttcctcacgg ctggcgaatg cctgcaacgt gccggtgtct actatcactt cgcgtcgttc
     1501 ctgttcgtgc atgaccaggc gcagatgaag gctgcacaca agaagcagat cgagtgccgg
     1561 caggctgcgt tgccgcatct ccggccgccc ggcgagcgcg tcgagattcc gtaccagggc
     1621 aaaacgttgg cgggcatcct gcgcaagccg ggaggcatcg agcggccgcc tgtcgtaatc
     1681 atgtcggtcg gactcgattc gaccaaggag gaaggtgacg cctacgaagc gccgttcctg
     1741 gcgcgtggca tggcaacgct gatcttcgaa ggcccgggcc agggcgaggc gcaatatgac
     1801 tttgccattc gcggtgacta cgaagtacca gtgaaggccg taattgattt cgtggaaaag
     1861 cgctccgatc tcgatgccaa gcgcgtcggc aggtggggcg tgagcctggg cggctattac
     1921 gcgccgcgcg ccgcagcctt cgacaagcgc atcaaggcct ccattgcgct cggcggaccg
     1981 ttcaactggg gcgcggcct
//
LOCUS       DBNGZE010000034         4245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_422502, whole genome shotgun sequence.
ACCESSION   DBNGZE010000034 DBNGZE010000000
VERSION     DBNGZE010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_422502"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="AC16FV_00895"
     CDS             complement(<1..164)
                     /locus_tag="AC16FV_00895"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR2848652.1"
                     /translation="MRIGHGYDVHPLVAGRDLILGGVKIPHSKGLQGHSDADVLIHAI
                     CDACLGAAGLG"
     gene            complement(164..883)
                     /gene="ispD"
                     /locus_tag="AC16FV_00900"
     CDS             complement(164..883)
                     /gene="ispD"
                     /locus_tag="AC16FV_00900"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR2848653.1"
                     /translation="MSTADAIWAIVPAGGQGRRMQAAFPKQYLPLLGRPVILHTLTRL
                     CAHPRVRGVLAGVPNQDMHWQKIAAQAARLPKFLGSYPGGDTRAQTVMNGLKELAKHA
                     QDRDWVLVHDAVRPCVRLGDIDALIAAVGAEPEGGLLALPVSDTVKRVDKEGHVTETV
                     KRDGLWRAATPQMFRIDVLTPALEQALRNGMDVTDEASAIEAAGGHPRVVACHSDNIK
                     ITLPEDLMLAELYLKQQQVRA"
     gene            complement(905..1414)
                     /locus_tag="AC16FV_00905"
     CDS             complement(905..1414)
                     /locus_tag="AC16FV_00905"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR2848654.1"
                     /translation="MATKRPRFKVGETVFYPSAGVGVILAVEDIYLSGQVESCFVIRI
                     QDTQMTVKVPKSNLEKSGIRPLLDGKKLKELFKILSAKGKRRVTGGNWAERCKDIERR
                     INSGSCLELGEVVRDLTRWKKQSGLSFEESMLLETANSYLAREVAAVQGIAPDAARDR
                     ILSYIEERA"
     gene            1702..>4245
                     /locus_tag="AC16FV_00910"
     CDS             1702..>4245
                     /locus_tag="AC16FV_00910"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000716 - transcription-coupled
                     nucleotide-excision repair, DNA damage recognition
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR2848655.1"
                     /translation="MAIASAAQGHSGPLLVITASSFSAQRLEDEIRFYAPELPILLFP
                     DWECLPYDVFSPHQDIISQRLETLHRLPDLTRGVVLASMATLLHRLAPRDFISGHSFL
                     LQGGDRLDIQRLREQLTRANYHSVSQVMEAGEFAVRGGLVDLFPTGSHHPYRLDLFGD
                     QLESIREFDAATQRSGKSIDAIRLLPAREYPLTEASIQHFRQSFRARFEGDPQKVPLY
                     RDISKGLAPSGAEYYLPLFFPEMASIFDYLPESALFLMEAGVTEIAENFRRETEQRYG
                     EMRHDRERPLLEPGEIFIATNEFFNRVEAWPCVEMVDSDVAQAGEETQPDSKVVAYGT
                     RPPASLPVDYKSTSPYGTLFNYLRDFTGRILLVAESTGRRETLRGLLQEHGLPPEDIS
                     GWRAFLDSNAKLALAVADLEKGLLLKEPEIAVITEAQLYGERAAQRRRRNRAEREPEA
                     IIRSLAELKIGDPVVHEDHGVGRYLGLQTLDIGDGPTEFLALEYADGDKLYIPVLALH
                     LISRYTGTDPENAPLHKLGGEAWEKAKRRALEKAHDAAAELLEICALRAAREGRAFPP
                     PDEHYQSFASTFPFEETPDQERAIQEVLKDMQTAKPMDRLVCGDVGFGKTEVAMRAAF
                     VAAMGKAQVAVLVPTTLLAQQHYQNFQDRFAGLPFRIELLSRVRSQSEQRGILAALAD
                     GSVDIVIGTHRLLQDDVRFKHLGLVILDEEHRFGVRQKERLKKLRSEVDILTMTATPI
                     PRTLNLSLSGLRDISLITTAPEGRLSVKTFISEKNRSVIREACLREIRRGGQVYYLYN
                     EVRTIDRAARELQELVPEADIRIAHGQMPEHDLERIMLDFYHQRFNVLVC"
BASE COUNT          850 a         1320 c         1234 g          841 t
ORIGIN      
        1 ccgagaccgg cggcgccgag gcaggcatca cagatagcat gaatcagaac atcggcgtcg
       61 gaatggccct gcaaaccctt gctatgcgga attttcacgc cgccgagaat cagatcgcgt
      121 ccggcaacca gcgggtgcac gtcataaccg tgaccgatcc gcatcatgcc cggacctgct
      181 gttgtttcag atacagctcc gccagcatga gatcttccgg cagcgtgatc ttgatgttat
      241 ccgaatgaca ggccaccacg cgcggatgtc cgcccgcggc ctcgatcgcg gaggcttcgt
      301 cggtgacatc catgccattt cgcagcgcct gctccagggc gggcgtcaac acgtcaatcc
      361 gaaacatctg tggtgtcgcc gcgcgccaca gtccatcgcg tttcaccgtt tcggtcacat
      421 ggccttcctt atccacgcgc ttgaccgtat cggaaaccgg caatgccagc aagccgcctt
      481 cgggttccgc gccgactgcc gcaatcaagg cgtcgatgtc acccagtcgc acgcacgggc
      541 gtactgcatc gtgcaccagc acccaatcac ggtcctgggc gtgcttggcc aattccttga
      601 ggccgttcat caccgtctgc gctcgagtat cgccgccagg gtagctgccg agaaatttgg
      661 gaagacgtgc cgcctgtgcg gcgatctttt gccagtgcat atcctgattc ggaacgccgg
      721 ccagcacgcc acgcacgcgc gggtgagcgc acaggcgcgt caaggtgtgc aggatgacgg
      781 gacgaccgag aagcgggaga tactgtttag gaaatgccgc ctgcattcgg cggccctggc
      841 cacccgcggg aacgatggcc cagatggcgt cagcggtgct caagcggaat gacgcctgaa
      901 cctctcaggc gcgctcttcg atgtaactca ggatgcgatc cctggcagcg tccggagcaa
      961 tgccctgcac cgcggcgact tcacgcgcga ggtagctgtt ggcggtttcc agcagcatgg
     1021 attcctcgaa cgacaagccg gattgcttct tccagcgcgt caggtcgcgc accacctctc
     1081 ccagctccag gcaggagccg gaattgatgc ggcgctcgat gtccttgcag cgctcggccc
     1141 agttaccgcc ggtaacgcgg cgttttccct tggcggaaag aatcttgaac agttccttga
     1201 gttttttgcc atccagcaag ggacggatgc cgcttttctc cagattagat ttcggcacct
     1261 tgacggtcat ctgggtgtcc tggatgcgga tgacaaaaca ggactcgacc tggccggata
     1321 aataaatgtc ctcgacggcc aaaataacac ctacacctgc ggaaggataa aaaaccgttt
     1381 caccaacctt gaagcgtggg cgttttgtcg ccattcagcc atcctcctgg gttgaactgc
     1441 tatcgatact taggggcatc tattgtcaat ctggattaaa ttatgcccgt aacccccaac
     1501 tctgacaaga cttttgagga ttcgacccac ggtcatcccg agcgcttctt gaggtagaat
     1561 tgccgacctt gcggccctgt ccgggttgcc cgtttgccca tttttaatag acatcatttg
     1621 gaatcgcccc tgaaacctaa gttccccgcc ggacgtggcg ccagactctc gtggcgcggt
     1681 ctggccggca gcgggcgtgg tttggctatc gcctcagctg cccaaggcca tagcggcccg
     1741 ctgctggtaa ttactgccag cagcttctcg gcccagcggc tggaagacga aatccgtttt
     1801 tacgcccccg aattgcccat cctgttgttc ccagactggg aatgtctgcc ttacgacgta
     1861 ttttccccgc accaggacat catttcgcaa cggctcgaaa cgctgcaccg gctgccggac
     1921 ctgacgcggg gcgtggtgct ggcttccatg gccacgctgc tgcaccggct tgcaccgcgc
     1981 gactttatca gcggacacag tttcctgctc cagggcggcg accggctcga tattcagcgc
     2041 ttgcgtgaac agctgacgcg cgccaactac cacagcgtgt cgcaggtcat ggaggccggc
     2101 gagtttgccg tgcgcggcgg actggtggac ctgttcccga ccgggagtca ccacccctat
     2161 cgcctggacc tgttcggcga tcagctggaa tccatccgcg agttcgatgc cgccacacaa
     2221 cgctcgggaa aatccatcga cgccatccgg cttctgccgg cgcgcgaata cccgctgacg
     2281 gaagcatcga tccagcattt ccggcagtcc ttccgcgcgc gtttcgaagg cgatccgcag
     2341 aaggtaccat tgtaccgcga tatcagcaaa ggcctcgccc cctcgggcgc ggaatattac
     2401 ctgccgctct tctttccgga gatggcaagc atttttgatt atctaccgga atccgcgttg
     2461 ttcctgatgg aggccggtgt gactgagatt gctgaaaact tccggcgtga aaccgaacaa
     2521 cgctatggcg aaatgcgcca tgaccgggaa aggcccttgc tggaaccggg cgagatattt
     2581 atcgccacaa atgaattttt caacagagta gaggcgtggc cgtgcgttga aatggtggat
     2641 tcagatgttg cgcaagccgg agaggagaca cagcctgatt ccaaagtagt ggcctatggc
     2701 acgcgcccac ccgccagtct tccggtggat tacaaatcca cctctcctta cgggaccttg
     2761 ttcaattatc tgcgcgactt tacggggcga atcctgctgg tcgcggaatc caccgggcgg
     2821 cgcgaaacgc tgagggggct gctccaggag catggtctgc caccggaaga tatttccgga
     2881 tggcgtgcat tcctcgatag caacgcgaag cttgcgctcg ccgtggccga tctggaaaag
     2941 ggtctgttgc tcaaggagcc ggaaatcgcc gtcattaccg aggcacaact atacggcgaa
     3001 cgtgccgcgc agcgacgccg acgcaatcgc gccgagcgcg agcccgaggc aatcattcgc
     3061 agtctggcgg agctgaaaat cggcgacccc gtggtgcatg aagatcatgg cgtcgggaga
     3121 tatctcggat tgcaaacgct cgacatcggc gatggaccga cggagtttct ggcgctcgaa
     3181 tatgccgacg gcgacaagct ctacatcccg gtcctcgcgc tgcacctgat cagccgttac
     3241 accggaaccg atccggaaaa cgcgccgctg cataaactcg gcggcgaggc ctgggaaaag
     3301 gccaagcgtc gtgccctcga aaaggcgcat gatgcagccg cggaattgct ggaaatctgt
     3361 gccctgcgcg cggcacgcga aggccgggcc ttcccgccgc cggatgagca ttaccagtct
     3421 tttgccagca ccttcccctt cgaggaaaca cccgaccagg aacgcgctat ccaggaagtg
     3481 ctgaaggaca tgcaaaccgc caagcccatg gaccggctgg tctgcggcga cgtcggcttc
     3541 ggtaagaccg aagtcgccat gcgcgccgcc tttgtagccg cgatgggcaa ggcgcaggtg
     3601 gcggtgctgg taccgaccac cctgctggcg cagcagcatt atcagaattt ccaggaccgc
     3661 tttgctggtc tgccgtttcg catcgagttg ctgtcacggg ttcgcagtca gagtgaacag
     3721 cgcgggattc ttgccgcgct cgcggacggg tccgtggaca tcgtcatcgg cacccatcgt
     3781 ttactgcaag acgatgtgcg tttcaagcac ctggggctgg tcatcctcga tgaggagcat
     3841 cgtttcggcg tgcgccagaa ggaacgtctg aagaaactgc gcagcgaggt cgatatcctg
     3901 accatgacag ccacacccat cccccgcacc ttgaacctgt cgctctcggg cctgcgcgat
     3961 atctcactga tcaccaccgc tccggaaggc cgcctgtcgg tcaagacctt catcagcgag
     4021 aagaaccgct cggtcatccg cgaggcctgc ctgcgtgaaa tccggcgcgg cggccaagtg
     4081 tattacctgt ataacgaagt ccggactatc gacagggcgg cgcgcgagct tcaggagttg
     4141 gtgcccgagg ctgacatccg catcgcccat ggtcaaatgc cggaacatga cctcgaacgc
     4201 atcatgctcg acttctacca ccagcgcttc aacgtgctgg tgtgc
//
LOCUS       DBNGZE010000035         3641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1325873, whole genome shotgun sequence.
ACCESSION   DBNGZE010000035 DBNGZE010000000
VERSION     DBNGZE010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1325873"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="AC16FV_00915"
     CDS             <1..1256
                     /locus_tag="AC16FV_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR2848656.1"
                     /translation="STLQRREVPPTRRPNAPRALVIAPTRELAVQIHKDAEVLGKHTG
                     FKLGLVFGGTGYDTQRKMLEEGVDVLIGTPGRIIDYFKQHVFDLNAVQVMVLDEADRM
                     FDLGFIKDIRFLLRRMPPPAKRLSMLFSATLSYRVTELAYEHMNNPQFVSIEPDKRTC
                     DKVTQVLYHTALEEKLPLLVHLLRQKDPTRSLVFTNTKDMAENIAATLKGNGLNAAVL
                     SGDVPQNQRLRILLDFTEGRLPVLVATDVAARGLHIPDVSHVFNYDLPQNAEDYVHRI
                     GRTARAGASGDAVSFACERFVFGLPEIEAYIGHKIPTAPVMPEMLPTLQPPVKREYHP
                     RPGGDRGRGGRPERGRGRDREGRRPGRERQREPEVRRPQAEPRAERPTEVQPSAPAPA
                     KPLPSAKRLIRPALRRSGGEKPVIG"
     gene            1280..1837
                     /locus_tag="AC16FV_00920"
     CDS             1280..1837
                     /locus_tag="AC16FV_00920"
                     /EC_number="1.13.11.53"
                     /EC_number="1.13.11.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0010308 - acireductone dioxygenase
                     (Ni2+-requiring) activity [Evidence IEA];
                     GO_function: GO:0010309 - acireductone dioxygenase
                     [iron(II)-requiring] activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-dihydroxy-3-keto-5-methylthiopentene
                     dioxygenase"
                     /protein_id="HIR2848657.1"
                     /translation="MARIVFPDGKELEDLSAVQSRLARLGITLRNWPAPADARAKELM
                     EKKSLNDAEKEELLGFVQNRFEELKREKGYQTRDMVVIHEDIPGLADMLAKFDKIHTH
                     ADDEVRYILAGSGYFGFVEPDGGQFLLEVSGGDYINVPANAEHWFEMRGCTRCKAVRY
                     FIDTSGWTPNYTGRTCEFAEASATA"
     gene            complement(1916..>3641)
                     /locus_tag="AC16FV_00925"
     CDS             complement(1916..>3641)
                     /locus_tag="AC16FV_00925"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HIR2848658.1"
                     /translation="PRFDPYTVTTTLTGIGTTTQTIAGSATAQQYHLKTERDAMTLGF
                     NKEISDALGVQVSFRTEEKDGERLFGQGTFGTWRFLTDPIDQTTRQVDATFSYTTEAL
                     QLTGGYYGTSFENRNNLLNVVGATLFTGSNEIALPPDNESHQLHLAGGYNFTKTTRGT
                     FKLAYGRITQNETYATIPVAGAPDNLDGRIDTTLVQAGLTARPMPKLSLRADVRYEDR
                     DDKTPVFVYFPSNFATPTTATSDGTNEPRDIKTTSGKFEASYQLPMALRLTGGVDYVE
                     KTRNSPPVRSVSFRETTDETSLRIELRRSISETLTGALSYIQSKRGGSEWLVNTFVDS
                     SVTATSSNQVAPLHLADRDRDMWRLVLNWMPTDPLSLNLRLDQARDEYRGRGFSVVDQ
                     AVREGRAENYSLDAAYSFSDDVQGTVWYSVNDTSLESAQCRSNNGDVCTPGTQQVWGS
                     DVRNLADSFGLGLRAKLNPKLELSADVYQSKVRDEIELNSVAGAAVTPIDDVINTKVT
                     SVKLSAKYALERNAGVRVMYIYDRYRTNDWTWANWNYSPADGGTTVLQEPEQKVNFVG
                     VAYYYRWR"
BASE COUNT          653 a         1141 c         1169 g          678 t
ORIGIN      
        1 attcgacgct gcagcgtcgc gaagtcccgc cgacccggcg cccgaacgcg ccgcgcgcac
       61 tcgtgatcgc gccgacgcgc gaactcgcgg tgcagatcca caaggacgcc gaggtgctgg
      121 gcaagcacac gggcttcaag ctcggtcttg tgttcggcgg caccggttac gacacccagc
      181 gcaagatgct ggaggagggc gtagatgtgc tgatcggcac gcccgggcgc atcatcgatt
      241 atttcaagca gcacgtgttc gatctcaatg ccgtgcaggt catggtgctc gacgaggccg
      301 accgcatgtt cgacctcggc ttcatcaagg acatccgttt tctgctgcgg cgcatgccgc
      361 cgccggccaa gcgcctgagc atgctgttct cggccacgct ctcctaccgc gtcaccgagc
      421 tggcctacga gcacatgaac aacccgcagt tcgtgagcat cgagccggac aaacgcacct
      481 gcgacaaggt cacgcaggtg ctgtaccaca ccgctctcga ggaaaaactg ccgctgctgg
      541 tacacctgct gcggcaaaag gacccgacgc gcagcctggt gttcaccaac accaaggaca
      601 tggcggagaa catcgccgcc acgctcaagg gcaacggcct caacgccgcg gtgctttccg
      661 gcgacgtgcc gcagaatcag cgcctgcgca tcctgctcga cttcaccgag ggccggctgc
      721 cggtgctggt ggcgacggat gtggcggcac gcgggttgca catcccggat gtgtcgcacg
      781 tgttcaacta cgacctcccg cagaacgccg aggattatgt gcaccgcatc ggccgcaccg
      841 cgcgcgccgg cgccagcggt gacgccgtga gttttgcctg cgagcgtttt gttttcgggt
      901 tgcccgaaat cgaagcctat atcggccaca agattccgac cgcaccggtt atgccggaaa
      961 tgttgccgac gttgcagccg ccggtaaaac gtgaatacca tcctcgtccc ggtggggatc
     1021 gcggacgcgg tggccgcccc gagcggggac gtggccggga tcgcgagggc cggcgtccgg
     1081 ggcgtgaacg tcagcgcgaa ccggaggtcc gacgtccgca agcggagccg cgcgcggagc
     1141 ggccgacgga agtacagcca tccgccccgg caccggccaa gccgctacca agcgcgaagc
     1201 gtctgatcag accggcgctg cgccgctccg gcggcgagaa accggttatc ggttaatttt
     1261 ttactggagg ataaagatca tggcccggat cgtttttccc gatggaaaag aactggaaga
     1321 cctctccgcc gttcagagcc ggttggcgcg tctgggcatc acgctgcgca actggcccgc
     1381 gcccgcggat gcgcgcgcga aagagctgat ggaaaagaaa agcctgaacg acgccgagaa
     1441 ggaagagttg cttggttttg tgcagaaccg cttcgaggaa ctgaagcgcg agaagggcta
     1501 ccagacgcgc gacatggtgg tgatccacga ggacatcccg ggactcgccg acatgctcgc
     1561 caagttcgac aagattcaca cccacgcgga tgacgaggtg cgctacatcc tcgccggctc
     1621 cggctacttc ggtttcgtcg agccggatgg cgggcagttc ctgctcgagg tgagcggtgg
     1681 cgactacatc aacgtcccgg ccaacgccga gcactggttc gagatgcgcg gctgcacccg
     1741 ctgcaaggcg gtgcgctatt tcattgacac ctccggctgg acgccgaatt acaccggccg
     1801 cacgtgcgag ttcgcggagg cgtcggcgac ggcctagcac atcaaccgcg gccgtttttc
     1861 ctctcgcaaa aaaacggccc gccgaagcgg gccggttggg gtcagttggt tcgctctacc
     1921 gccaccggta ataatacgcc acaccgacga agtttacctt ctgttccggc tcctgcagga
     1981 cagtggtgcc gccatcggca gggctataat tccagttggc ccaggtccag tcgttggtcc
     2041 ggtaacgatc atagatgtac atcacccgca cgcccgcatt gcgctccagt gcatacttcg
     2101 ccgaaagctt tacagacgtg accttggtgt tgatgacgtc atcaatcggg gttactgccg
     2161 cgccggcgac agagttcagc tctatctcgt cgcgcacctt cgattggtac acatccgcgc
     2221 tcagctcgag cttcgggtta agcttggcgc gtagcccaag gccgaaggag tccgccaggt
     2281 tgcgcacgtc ggaaccccat acctgctggg tccccggagt gcagacatcc ccgttgttgc
     2341 tgcggcactg cgcgctttcc aggctggtgt cattgaccga ataccagaca gtcccctgca
     2401 cgtcgtcgga gaagctgtaa gccgcgtcca gcgagtagtt ctccgccctg ccctcgcgca
     2461 ccgcctggtc gacgacgctg aagcctcgcc cgcgatactc atcacgcgcc tggtcgagac
     2521 gcaggttgag cgacagggga tcggtcggca tccagttcaa taccagtcgc cacatgtcgc
     2581 ggtcgcgatc ggcaaggtgc agcggcgcga cctggttgct ggaggtggcg gtcaccgagc
     2641 tgtcgacgaa agtattaacg agccattcgg agccgccgcg tttactctga atgtacgaca
     2701 acgcgccggt cagggtctcg gagatcgagc ggcgcagctc gatgcgcaat gacgtctcgt
     2761 cggtcgtttc gcggaagctg accgagcgta ccggcgggct gttgcgcgtc ttctccacgt
     2821 agtccacgcc accagtcagg cgcagggcca tcggcagctg atagctcgct tcaaacttac
     2881 ccgaggtggt cttgatgtcg cgcggttcat tggtgccgtc gctcgtggcg gtcgtcggcg
     2941 tggcgaagtt cgaggggaaa tacacgaata ccggggtctt gtcgtcgcgg tcctcgtaac
     3001 gcacatccgc gcgcagcgac agcttcggca tcgggcgtgc ggtcagaccg gcctgcacga
     3061 gggtcgtgtc gatgcgcccg tcgaggttgt ccggggcgcc ggcgacggga atcgtagcgt
     3121 acgtctcgtt ctgcgtgatc ctgccgtagg cgagcttgaa ggttccgcgg gtcgtcttgg
     3181 tgaagttgta gccgccggcc agatggagct ggtgcgactc gttatccggt ggcaaggcaa
     3241 tttcattgct gcccgtgaag agcgtggcac ccactacgtt gagcaggttg ttccggtttt
     3301 cgaacgatgt cccgtagtaa ccacccgtga gctgcaaggc ctcggttgtg tagctgaacg
     3361 tggcgtcgac ctgccgcgtc gtctggtcga tcgggtcggt caagaagcgc caggttccga
     3421 aggtgccttg gccaaagagt cgctccccgt ccttttcctc ggtgcggaag ctcacctgca
     3481 cgcccaaggc gtccgagatc tccttgttga atccgagcgt catggcatcg cgctcggtct
     3541 tgaggtggta ttgctgcgcc gtcgcgctgc ccgcgatagt ctgcgtggtg gtgccgatgc
     3601 cggtgagcgt agtcgtcacg gtgtacgggt cgaaccgcgg c
//
LOCUS       DBNGZE010000036         3955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1326022, whole genome shotgun sequence.
ACCESSION   DBNGZE010000036 DBNGZE010000000
VERSION     DBNGZE010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1326022"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1497
                     /locus_tag="AC16FV_00930"
     CDS             61..1497
                     /locus_tag="AC16FV_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2848659.1"
                     /translation="MNNRTILVVDDQSTHRDLSRSALEDAGFTVQTAASGPEALERLN
                     NTAFALVVTDQIMPDMSGLELLREIRGQWARLPVVIVTAHGTVETAVDAMKAGASDFI
                     TKPFTPEELLLVVRRVLEHQDLVEEVRTLRERVGDLYRFDRIVSKSPKMTEIFEMVRN
                     LADLDTTVLITGETGTGKELVAQAIHHNSHRRDQRFVRINCGALTETLLESELFGHER
                     GAFSGAVQARRGKFEYASGGTLLLDEIGDISPAMQLKLLRVLQEKEIQRVGGNETITV
                     DVRILATTNKDLEEAMAAGVFRSDLYYRLNVARIHLPPLRERMEDVPLLAEHFLRSFC
                     DKTGRPLRGMERDAMNAMMDYDWPGNVRELANVIERAAVMVPGDRITAVELPARPARP
                     PTGEPVCFNTDLPLKEGRQRVIADYEKTYLVQCLRRYRGNISQSAQHCGVDTKTFYRK
                     MQEYGLDKRDFKKTEETVEHDSQHQLIP"
     gene            1786..3894
                     /locus_tag="AC16FV_00935"
     CDS             1786..3894
                     /locus_tag="AC16FV_00935"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR2848660.1"
                     /translation="MKISRLRKTTLALGITALASVVSVPTVFAGSGKVTLIQTGDIHG
                     HLLPRPNLRSDATGSSMEGGVARMYTVIKEMRKDATKCTTVNGVKTCTDYSLLLNTGD
                     TLQGSGEALFSRGQAMIDVLNNFGFVAHAPGNWDFLYGPARFEETFKGTATTPPLANW
                     NAMASNLYYTNQFDDAAVCGVTATDPATGTVRPRKRVLPTYMIKQVGNLKVGILGFTT
                     ARAIAAVGTSVTKNYMFTDGKTEVPCFVDRLRNVEGVDLVVMMSEMEMSRDIQIAETL
                     APGPDVILNSDMHERTVAPIVAVNPDGHQTLIVEEGQDGTVIGKMELEVVNGKITEWE
                     FKQRVITQDIKEDKTIAAAVAKARAPFVTGTFVPGQTVTVGGNTTMLMRPVDEVIAYT
                     QVGLHRSNFVDEDMPGVVEGTSHDLIVDAMAAIGQAGSASIRGFRYGTHVPPGGGITM
                     EDIYHFIPIAAKLGRTDKACGADLKFAIENSIGGTFHPDPGQWTGGWMFGYSGLTMDV
                     DGCSGFMGATPVNPSPLTFANRYRPWPTARGTNIKVNGVAVDDLEIYDNRATIKDPVT
                     GLDVPNPTFQKCLSGDGSNTPRDGYMVTGYWYADDPTTINNCNPCRGRVVQVVKTDGS
                     IAQVSGPGASGTLPNSLEVMDITEAVVKYLRENLGGVVTADNLPTHRLTVKRLPTINP
                     YSFRQIQPLKGASSATCPAL"
BASE COUNT          845 a         1226 c         1147 g          737 t
ORIGIN      
        1 tgacagcgcc gaaggccggg gaaccacttt tacttttgtc ctgccggaga cgccgacccg
       61 atgaacaatc gcaccatcct ggtcgtcgat gaccagagca ctcaccgtga tctcagccgt
      121 tcggcgctgg aagacgccgg tttcaccgtg caaacggccg cttccggccc cgaggcgctg
      181 gaacggctga acaacaccgc atttgcgctc gtggtcaccg accagatcat gccggatatg
      241 tcagggctcg aactgttgcg ggaaatccgt ggtcaatggg cgcggcttcc cgtggtgatc
      301 gtaacagcgc acgggaccgt tgagaccgcc gtggatgcaa tgaaggccgg tgccagtgat
      361 ttcattacga aacccttcac gccggaagaa ttgctgctcg tggtacggcg tgtcctggaa
      421 caccaggacc tggtggaaga agtgcgcacg ctgcgcgagc gcgtcggcga tctataccgt
      481 ttcgaccgca ttgtcagcaa aagtccaaag atgaccgaaa tcttcgagat ggtccgcaac
      541 cttgccgacc tcgataccac ggtattgatt accggcgaaa ccgggaccgg aaaggaactg
      601 gtcgcacagg caattcacca caacagtcac cgacgggatc agcgttttgt ccgtatcaat
      661 tgtggcgcat tgacggagac tctgctggaa agcgagctat tcgggcacga acgtggcgcg
      721 ttttccggcg ctgtacaggc tcgacgcgga aagttcgaat acgcttcagg cggtaccctg
      781 ctgctggatg aaatcggcga catctctccg gccatgcagt taaagctatt acgcgtcctg
      841 caggaaaagg agatccagcg cgtgggaggt aacgagacca tcaccgtcga tgtgcgtatc
      901 cttgcgacga cgaacaaaga cctggaagag gcgatggcgg caggagtatt ccgttcggat
      961 ctgtactacc ggctgaacgt ggcgcgcatt catctaccgc cgttgcgcga gcgcatggag
     1021 gatgtacccc tgctcgctga acattttctg cgctccttct gcgataagac cggccgtccg
     1081 ttgcgcggca tggagcgtga tgcaatgaat gcaatgatgg attatgactg gcccggcaac
     1141 gtgcgtgagc tggcgaatgt gatagagcgt gcggcagtca tggtcccggg tgaccggata
     1201 acagcggtgg agttgcccgc gcgccctgcg cgcccaccga ccggcgaacc agtctgtttc
     1261 aatacggatc ttccgctcaa ggaaggcaga cagcgtgtta tcgcagatta tgaaaaaacc
     1321 tatctggtcc agtgcctgcg ccgttaccgg ggcaacatca gccagagcgc acagcactgc
     1381 ggcgtcgaca ccaaaacgtt ttaccgcaaa atgcaggaat acgggctgga caagcgcgac
     1441 ttcaaaaaaa ccgaagaaac tgtcgagcac gattcgcaac accagctgat tccataattt
     1501 cagccgtcgg catcacaacc ctctgcttgc ccggcaaatc gggcatggcc ggtataatcg
     1561 ggtgttcctg cccgcccgtt catgcccgaa cttcatcgca gcacaaacta accaccgaca
     1621 tgggcatgct aacgcgctaa ttgcgcaatg aatatttctt cggtggcaat actgatcgtg
     1681 ccactacgta cggcacaact cttgctcctg atggcgtcat ccatgccccc gcgacggggc
     1741 atggttgaac cagaaacact ctcataacct ggaggagcat tactcatgaa aatttcccgt
     1801 ttgaggaaga cgacgctggc gcttggcata accgccctgg cctccgtcgt atccgtgccc
     1861 acggtattcg ccggctcggg caaagtgacc ttgattcaga ccggtgacat ccatggccac
     1921 ctgttaccgc ggcccaacct gcgcagcgac gcaacaggca gcagcatgga aggcggcgtg
     1981 gcacgcatgt acacggtcat caaggagatg cgcaaagacg cgaccaagtg caccacggtc
     2041 aatggcgtca agacatgcac agactattcc ctgctgctca acaccggcga tacgttgcag
     2101 ggatcgggag aggcgctgtt ttcccgcggc caggccatga tcgacgtgct taataacttc
     2161 ggatttgtgg cccacgcccc cggcaactgg gacttcctct acgggcccgc ccgtttcgag
     2221 gagaccttca agggcaccgc gaccaccccg cccctggcca actggaatgc gatggcgtcg
     2281 aatctttatt acaccaacca gttcgacgac gcggccgtgt gcggcgtgac ggcaacggat
     2341 cccgccaccg gcacggtgcg tccgcgcaag cgtgtcctgc cgacctatat gatcaagcaa
     2401 gttggcaacc tcaaggtcgg cattcttggc tttactaccg cgcgcgccat tgccgcggtc
     2461 ggcaccagcg tgaccaagaa ctacatgttc accgacggca agaccgaagt gccctgcttc
     2521 gtcgacaggc tgcgtaacgt cgagggcgtc gatctggtgg tgatgatgtc cgaaatggaa
     2581 atgtcccgcg acatccagat agccgagacc ctcgcccccg gaccggacgt gatcctcaac
     2641 tccgacatgc acgagcgcac cgtcgccccc atcgtcgcgg tgaaccccga cggtcaccag
     2701 accctgatcg tcgaagaggg ccaggacggc accgtgatcg gcaagatgga actcgaggtg
     2761 gtgaacggca agatcaccga gtgggaattc aagcagcgcg tgattaccca ggacatcaag
     2821 gaagacaaaa ccatcgccgc cgcggttgcc aaggcgcgcg cgcccttcgt gaccggcact
     2881 ttcgtgcccg gccagacggt gacagtgggt ggcaacacca ccatgctgat gcgcccggtg
     2941 gacgaggtga tcgcctatac ccaggtgggc ctgcaccgct ccaacttcgt ggatgaggac
     3001 atgcccggcg tggtcgaagg cacgtcgcat gatctgatcg tcgacgccat ggctgccatt
     3061 ggccaggccg gatcggcgtc catccgcggc ttccgctatg gcacccacgt gcccccgggc
     3121 ggcggcatca ccatggagga catctaccac ttcatcccca ttgccgccaa gctgggtcgc
     3181 accgacaagg cctgcggcgc cgacctgaag ttcgccatcg agaattcgat cggcggcact
     3241 ttccatcccg atccgggcca gtggaccggc ggctggatgt tcggctacag cggcctgacc
     3301 atggacgtgg atggctgcag cggcttcatg ggcgccacgc cggtgaatcc gtcgcccctg
     3361 acctttgcca atcgctaccg gccctggccg actgcccgcg gcaccaacat caaggtcaat
     3421 ggcgttgcgg tcgacgacct cgaaatctat gacaaccgcg ccaccatcaa ggatccggtc
     3481 accggcttgg acgtccccaa cccgaccttc cagaagtgcc tgtcgggcga tggcagcaac
     3541 acgccccgcg atggctacat ggtcaccggc tattggtatg cggatgatcc gaccaccatc
     3601 aacaactgca acccctgccg tggtcgcgtg gtccaggtcg tgaagacgga tggcagcatt
     3661 gctcaggtct ccggaccggg tgcatccggt acgctgccca acagtctgga agtaatggac
     3721 atcaccgagg ctgtggtcaa gtacctgcgc gagaacctgg gtggcgtggt gacggctgac
     3781 aatctgccga cccatcgcct gacggtgaag cgtttgccca ccatcaaccc atacagcttc
     3841 aggcaaatcc agccgctcaa gggtgcctct tcggccacct gtccggctct gtaattttct
     3901 aaactggatt catccagtga cggcccggca tctgccgggc cgtttttttt tgcgt
//
LOCUS       DBNGZE010000037         3930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1464894, whole genome shotgun sequence.
ACCESSION   DBNGZE010000037 DBNGZE010000000
VERSION     DBNGZE010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1464894"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1398
                     /gene="hslU"
                     /locus_tag="AC16FV_00940"
     CDS             67..1398
                     /gene="hslU"
                     /locus_tag="AC16FV_00940"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR2848661.1"
                     /translation="MTPREIVQELDKHIVGQAAAKRAVAIALRNRWRRAQVADESLRA
                     EITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDSIIRDLV
                     DMAVKMTRVEEMAKVRTRAEDAAEDRILDILVPPAREVGFAVGEASRPSDGAARQMFR
                     KKLREGSMDDQEIEIELSTPAMGVEIITPPGMEEMTSQLQGLFQNMGGRRSRPRKVKI
                     REARKLLTEEEAGRLVNEDDIKGRALQRVEQHGIVFIDEIDKIVGRSETRGPDVSREG
                     VQRDLLPLVEGCTVSTKYGMVKTDHILFVASGAFHLSRPSDLIPEMQGRLPIRVELDP
                     LNADDFERILTEPDASLTEQYSALLATEGHTLEYTPDGVRRIAEVAWQVNERTENIGA
                     RRLHTVMERLLETVSFEAADRGHEKTVVNAAYVDQHLGSLVGNEDLARYIL"
     gene            1578..2039
                     /locus_tag="AC16FV_00945"
     CDS             1578..2039
                     /locus_tag="AC16FV_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2848662.1"
                     /translation="MTRLMRRGDDRAPLARWGLWDNDIDRMFQGFLSPMRWVEEAAGD
                     LVPAMDVKEREGEYVVKTEMPGVNKDDINITMENGVLTISGETRSETEEKEGERLIRQ
                     ERRYGKYTRSLRLGTQVDEKGIKANYKDGVLELILPKAEEVKPKKITVDVA"
     gene            2261..3154
                     /gene="argB"
                     /locus_tag="AC16FV_00950"
     CDS             2261..3154
                     /gene="argB"
                     /locus_tag="AC16FV_00950"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR2848663.1"
                     /translation="MTLTPQSAADRARILTEALPYIQRFQGKTIVIKYGGNAMESDNL
                     KRGFARDVVLMKLVGMNPVVVHGGGPQIGKLLERIGKKSEFIQGMRVTDRETMDVVEM
                     VLGGLVNKEIVNLISQQGGKAVGLSGKDGGLIRAKKMVLQKTNKENLPSEIIDIGHVG
                     EVSSIDPRLVALLDTGDFIPVIAPIGVGEDGQTYNINADVVAGKLAITLKAEKLMLLT
                     NTTGVLDKEGRLLTGLSINQVKDLISDGTIHGGMLPKVNYALDAVGSGVKTAHIIDGR
                     VEHAVLLEVFTNEGVGTLIKS"
     gene            3160..3744
                     /gene="slmA"
                     /locus_tag="AC16FV_00955"
     CDS             3160..3744
                     /gene="slmA"
                     /locus_tag="AC16FV_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR2848664.1"
                     /translation="MARPRRGERRQIILETLAHMLEKNQGEHITTAQLARAVGVSEAA
                     LYRHFASKAKMFEGLLEFIEETLFTRINKILAEESRSEPRVQNILFLLLGFADKNPGM
                     ARLLYGDVLVGETERLRQRVVQIYERIETQLKQILREGEAGENLRVPIADTAALLLAV
                     VEGSITRYVRSEFHSSPVAGWDKQWDLLRKGVFG"
BASE COUNT          870 a         1151 c         1199 g          710 t
ORIGIN      
        1 cgctgataat tttttttacc ccgtgacccc cgtggttaat aaagattagc taaaactatg
       61 tcggaaatga ccccgcggga aatcgttcag gaactggaca aacacatcgt cggccaggcg
      121 gccgccaagc gcgccgtggc tattgcgttg cgtaaccgct ggcgccgggc gcaggtcgcg
      181 gatgaaagcc tgcgtgccga gatcacgcca aaaaatattt taatgatcgg cccgaccggt
      241 gtcggcaaga ccgaaatcgc gcgccggctc gcccggctcg ccaacgcgcc ctttatcaag
      301 gtcgaggcga ccaaattcac cgaagtgggc tacgtcggac gcgacgtgga ttcgatcatc
      361 cgcgacctcg tggacatggc ggtcaagatg acgcgcgtcg aggaaatggc caaggtccgc
      421 acccgcgccg aagacgccgc cgaggaccgg attctggata tcctggtccc accggcgcgc
      481 gaggtgggtt tcgccgtggg cgaggcctcg cgcccgagcg acggcgcggc gcgccagatg
      541 ttccgcaaga aattgcgcga aggctcgatg gacgatcagg aaatcgaaat cgagctgtcc
      601 acgcctgcca tgggcgtgga gatcatcacc ccgcccggca tggaggaaat gaccagccaa
      661 ttgcagggcc tgtttcagaa catgggcggg cgacgttccc gcccgcgcaa ggtaaaaatc
      721 cgcgaggcgc gcaaactcct gaccgaagag gaggcgggcc ggctcgtcaa cgaagacgac
      781 atcaagggcc gggccttgca gcgcgtcgaa cagcacggca tcgtgttcat cgacgagatc
      841 gacaagatcg tcggccgctc cgaaacccgt ggcccggatg tctcgcgtga aggtgtgcag
      901 cgcgatctgt tgccgctggt ggagggctgc acggtctcga ccaagtacgg catggtcaag
      961 accgaccaca tccttttcgt tgcctcgggc gcgttccatc tttccaggcc ctcggatctc
     1021 atacctgaaa tgcaaggccg gttaccgatc cgggtcgagc tcgatccgct gaacgccgac
     1081 gatttcgaac gcatcctgac cgaacccgat gcctcgctca ccgagcagta cagcgccctg
     1141 ctggcgaccg aaggccacac ccttgaatac acacctgacg gcgtgcgccg catcgccgag
     1201 gtggcgtggc aggtgaacga gcgcaccgag aacatcggtg cgcgccgcct gcataccgtc
     1261 atggagcgcc tgctcgaaac ggtttccttc gaggccgccg accgcggcca tgaaaaaact
     1321 gtggtgaatg ctgcttatgt ggatcaacat ctgggcagtc tcgtcgggaa cgaagatctc
     1381 gcgcgctata tcctctgacg gctgccccgg ccgttttcaa ttcgtctgat tttccgcgat
     1441 ttttttcgcg cttgaaattt cccccgttgt ccccattaaa acatcgcgag acggcataac
     1501 gaacttgagg attgcgaggg caggttcaat gcaggccgta aatccaatta tttaaccgat
     1561 ttatctggag gtgttttatg acccgactga tgcgacgtgg agatgaccgt gccccgctgg
     1621 cccgctgggg actgtgggac aacgatattg accggatgtt ccagggcttc ttgagcccga
     1681 tgcgctgggt ggaagaggcc gcaggcgatc tggttccggc gatggacgta aaagagcgcg
     1741 aaggcgagta tgtcgtcaag acagaaatgc cgggcgtcaa caaggatgac atcaacatca
     1801 ccatggagaa tggcgttctg accattagcg gcgaaaccag gagcgagacg gaagagaagg
     1861 aaggcgagcg tctcattcgt caggagcggc gttacgggaa atacacccgc agcctgcgcc
     1921 tgggtacgca agttgacgag aagggcatca aggccaatta caaggacggg gtactggaat
     1981 tgatcctgcc caaggccgag gaagtgaagc ccaagaagat tacagtggac gtagcttaac
     2041 tcctccgctg ttgccgggac ggaccccgga gtgagaaagg ccgcgcaagc ggcctttctt
     2101 ttttatttca gtattctggc ctgctgactt taatgtgaat atgcgctaac atttcgatca
     2161 gaatgacggt tttccctcta actccccctc tcccgctaac gcgggagagg gtaaggggag
     2221 agggtgcaaa taaagtcaca agcaacaaaa tggaacaaca atgacgctca ccccccaatc
     2281 cgccgccgac cgggcgcgca tcctgaccga agcgttgcct tacatccagc gattccaggg
     2341 caagaccatc gtcatcaaat atggcggcaa cgccatggaa agcgacaacc tcaagcgcgg
     2401 tttcgcgcgc gacgtggtgc tcatgaagct ggtcggcatg aatccggtgg tggtgcacgg
     2461 tggtgggccg cagatcggca agctgctgga aagaatcggc aagaagagcg aattcatcca
     2521 gggcatgcgc gtcaccgacc gcgagaccat ggacgtggtt gaaatggtgc tcggtggctt
     2581 ggtcaataag gagatcgtca atctcatcag ccagcagggc ggcaaggccg tgggcctgtc
     2641 cggcaaggac ggcggattga ttcgcgccaa aaaaatggtg ctgcagaaga ccaataagga
     2701 aaatcttccg agcgagatca tcgacatcgg ccacgtgggc gaggtcagca gcatcgaccc
     2761 gcgcctggta gcgctgctcg acaccggcga tttcatcccg gtgatcgcgc ccatcggcgt
     2821 gggcgaggac ggccagacct acaacatcaa cgccgacgtg gtggccggca agctcgccat
     2881 cacgctcaag gccgagaagc tcatgctgct caccaacacc accggcgtgc tggacaagga
     2941 aggcaggttg ctcacgggcc tctccatcaa ccaggtcaag gacttgattt ccgacggcac
     3001 catccacggc ggcatgctgc ccaaggtgaa ttacgcgctc gacgccgtcg gcagcggcgt
     3061 caagacggcg cacatcatcg acgggcgtgt ggagcatgcg gtgttgctgg aagtattcac
     3121 caacgaaggc gttggcaccc tgatcaaatc ctgatcgcca tggcccgacc gcgccgcggc
     3181 gaacgccgcc agatcattct cgaaacgctg gcgcacatgc tcgagaaaaa ccagggcgag
     3241 cacatcacca cggcccagct cgcccgcgcc gtcggcgttt ccgaggccgc gctgtaccgc
     3301 catttcgcca gcaaggcgaa aatgttcgag ggcctgctgg aattcatcga agaaacgctg
     3361 ttcacccgca tcaacaagat cctcgcggaa gaatcccgct ccgaaccgcg cgtgcagaat
     3421 atcctgttcc tgctgctcgg cttcgccgac aagaaccccg gcatggcgcg tctgttgtat
     3481 ggcgacgtgc tggtgggcga aactgaacgc ctgcgccagc gcgtggtgca gatttacgaa
     3541 cgcatcgaaa cccaattgaa gcaaatcctg cgcgaaggcg aagccggcga gaatttgcgc
     3601 gtgccgatag ctgatacggc ggcgttgctg ctggccgtag tcgaaggctc gatcacgcgg
     3661 tatgtccgga gtgaattcca ctcatcgcca gtggcgggat gggataagca gtgggatttg
     3721 ttgcggaagg gtgtttttgg ttgagcgggc ttcgcccgcg catttgaatc gggttctcgc
     3781 acccgagggc gacgtccttt cttgtcgcga caagaaagga cgcaaagaaa cgccgtcccc
     3841 gagtctgcgc cggcttcgcc ggtttcactc ggcacatccc tgtgcctcgc ccttcgggca
     3901 gcccacaaac cggctgtgca aatcggctgt
//
LOCUS       DBNGZE010000038         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_6180, whole genome shotgun sequence.
ACCESSION   DBNGZE010000038 DBNGZE010000000
VERSION     DBNGZE010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_6180"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16FV_00960"
     CDS             <1..431
                     /locus_tag="AC16FV_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848665.1"
                     /translation="GVYAEMARTRPEAGENHTRAVTGLEYAFANTLTIGAELYYNGAG
                     TTDRARYDFARLFRGEIQNVARRYVGARLKYEFTPLLRTENYLIINRDDDSRFFAPSL
                     VYSLTQNWDWAVGAQFFGGVADSEYGRFYDVYYTSLQWFF"
     gene            701..1216
                     /locus_tag="AC16FV_00965"
     CDS             701..1216
                     /locus_tag="AC16FV_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848666.1"
                     /translation="MKPDYHRYRPMPLWGLVLIASLAGGMAEIIWVSLYASFAPLTAA
                     TVAREVTASLLPALAAGTTGAWLGILIHMLLAVALGYAFAYALWKPLARPHGMVATLA
                     ISVLTLATVWTMNFFVVLPVLNPVFITLMPYPVTFASKMLFALAMAVTLVWPELRRLA
                     REHTQTTRFAG"
     gene            1404..1619
                     /locus_tag="AC16FV_00970"
     CDS             1404..1619
                     /locus_tag="AC16FV_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR2848667.1"
                     /translation="MGINQTRFSVKGMKCGGCVAKATEVVSKLPGYETCEFDLKSASA
                     VVKGDVDPDAVAKALTQAGYPAQVDEG"
     gene            1651..>2586
                     /locus_tag="AC16FV_00975"
     CDS             1651..>2586
                     /locus_tag="AC16FV_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR2848668.1"
                     /translation="MTATVPEAATTRLSISGMSCAGCVATVENALKNVPGVTEAAVNF
                     AEHTATVHGRTDARTLVAAVTASGYGAAELRGPDDETEKEAAEFAHYRALLRKFAVAA
                     AVGFPLFVADPLDWLPHLATPPGYVFWSMVGLATLFVLAYAGGHFFRGAWKSFRAHNA
                     NMDTLIALGTGAAWVYSMLVVLFPDLVPSLARHAYFEAAAIIIALINLGQALEMRARG
                     KTSDAIKRLIGLQPKTARVIRDGREMDIPISEVGLDETLRVRPGEKIPVDGVIIEGHS
                     SVDESMLTGEPMPVEKKTGDEVVGGTFNKSGTFLFQ"
BASE COUNT          446 a          772 c          818 g          550 t
ORIGIN      
        1 ccggcgtcta tgccgaaatg gcccgcacgc ggcccgaggc cggtgaaaac catacgcgcg
       61 cggtcacggg cttggagtac gccttcgcaa acacgctcac cattggcgcc gagctctact
      121 acaacggcgc gggcacgacc gatcgcgcgc gctacgattt cgcgcgtctc ttcaggggcg
      181 agatccagaa cgtcgcgcgc cgctacgtcg gcgcgcgcct caagtacgag ttcacgccgc
      241 tcctgcgtac tgagaattat ctgatcatta accgcgacga tgacagccga tttttcgcgc
      301 cgagcctcgt gtattcgctc acccagaact gggactgggc ggtaggggcg cagttcttcg
      361 gtggcgtcgc cgacagcgag tacggtcggt tttacgacgt ctattacact tccctgcagt
      421 ggtttttctg atgcctgagg agtgatgccg atggtccacg atccggactg cctgatgaca
      481 ctcgacttgg gacgtgctca tgtgatgtgg ataatgatgg gcgggacggc cgtcgtgaaa
      541 aattcctcgc atacccaggc gttttctcaa caagcttccg aaatttcggc tcattccgtt
      601 ttgatatccg tcaaagtatg actattaggg ttcccctagg ctcgatgcgc ttttggtaaa
      661 ttaaaatcta cctgtcgccc atcgatcgaa ggagcacatc atgaagcctg attatcatcg
      721 ctaccgtccg atgccgcttt ggggcctggt tctgatcgcc tcgttggcgg gcggcatggc
      781 ggaaatcatc tgggtcagcc tctatgcttc gtttgcgccg ctgaccgccg cgacggtggc
      841 gcgcgaggtc accgcgagcc tgctgcccgc attagccgcc ggtaccaccg gtgcatggct
      901 cggtatcttg attcatatgc tgctcgcggt cgcgctcggc tatgccttcg cttatgcgct
      961 gtggaagccg ctcgcgcgtc cgcatggtat ggtggcgacg ctggcgattt cggtgctgac
     1021 gctggcgaca gtgtggacca tgaatttctt cgtggtgttg ccggtcctga atcccgtgtt
     1081 catcaccttg atgccctacc ccgtgacctt tgcctcgaag atgctgttcg cgctggccat
     1141 ggcggtgacg ctggtctggc ccgaactgcg ccggctggca cgggaacata cgcagaccac
     1201 gcgtttcgct ggttgagcca tttgtcgtaa ccgggtggac gccaggctga tgccggcgcc
     1261 cgctttcgcc gcgcgcagta aaatattgcc cggtgtatta accccctgtt cccgcaagga
     1321 agtttcaggg acgccgcgaa gcgcttgacc ttcccatggt tggaaggtct aaggtagttt
     1381 ttgtgttcat caaggagatt gtcatgggaa tcaaccaaac ccgtttttcc gtcaaaggca
     1441 tgaagtgcgg cggctgcgtg gcgaaggcga ccgaggtggt gtcgaagctg cccggttacg
     1501 agacgtgcga gttcgatctc aagagcgctt ccgccgtggt gaaaggcgac gtcgacccgg
     1561 atgccgtggc gaaggccctg acgcaggcgg gctaccccgc gcaagtcgat gaaggctgac
     1621 gcgacggtga cggtgccccg gagtccggtc atgaccgcga ccgttcccga agcggccacc
     1681 acgcgtctgt ccatctccgg catgagttgc gccggctgcg tagccacggt cgagaacgcg
     1741 ctgaaaaacg ttccgggtgt gaccgaggcc gcggtcaatt ttgccgagca cacggccacg
     1801 gtgcatggcc gcaccgatgc caggacactt gtggctgcgg tcacggcgtc gggttatggc
     1861 gcggccgagc tgcgcgggcc tgacgacgag acggagaagg aagcggcgga attcgcgcat
     1921 taccgcgcgc tgctgcgcaa gttcgcggtg gccgcggcgg tgggatttcc tctgttcgtg
     1981 gccgatccgc tggactggct gccgcatctg gcgacgccgc caggttatgt gttctggagt
     2041 atggtgggtc tggcgacctt gtttgtgctt gcctatgctg gcggacattt cttccgcggc
     2101 gcgtggaaat ccttccgcgc gcataacgcc aacatggaca cgcttatcgc gctcggcacc
     2161 ggcgcggctt gggtgtattc catgctggta gtgctcttcc ccgatctcgt ccccagcctt
     2221 gcgcgtcacg cctatttcga ggccgcggcc atcatcattg cgctgatcaa tctcgggcag
     2281 gcgctcgaga tgcgcgcccg cggcaaaacc tcggatgcga tcaagcgcct gatcggcttg
     2341 caaccgaaaa cggcgcgcgt tatccgggac ggccgggaga tggacatccc gatctccgag
     2401 gtcggcctcg atgaaactct gcgcgtgcgc ccgggtgaga aaatcccggt ggatggcgtg
     2461 atcatcgagg gccattcgag cgtggacgag tccatgctca ccggcgagcc gatgcctgtg
     2521 gaaaaaaaga ccggcgatga agtcgtcggc ggcacgttca acaagtccgg caccttcctg
     2581 ttccag
//
LOCUS       DBNGZE010000039         9263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_75947, whole genome shotgun sequence.
ACCESSION   DBNGZE010000039 DBNGZE010000000
VERSION     DBNGZE010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_75947"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      283..530
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            557..2602
                     /locus_tag="AC16FV_00980"
     CDS             557..2602
                     /locus_tag="AC16FV_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2848669.1"
                     /translation="MASPHSAFPSFAHCLSTTARVRAVCGGKVKMFYRRCIVAFVAVY
                     SLAMSVHAEPTSQDAIIVTATRNESPGIPIPAGISVISRGEIEASGARSLAELLRTRA
                     GVQVSDLYGDGSSATIDMRGFGATAVSNTLVLIDGRPLNNASDIGVLDLSLVDLNNVE
                     RVEIVQGSAGILYGNQAVGGLINIVTRRVPRRFEAGISAGYGSYDEQRLTAQVGQRFD
                     NGFAFSLGGRNRDSDNYRDHNHTKLDQARLRLEQTLSGGLVFLEYESSRENAQLPGSL
                     FKDELEANRRQSVSAYANDYNTTRTQTTRLGLAHALSENWQFEGEATHRDNDREFITG
                     FRFSPGTLSTQQRKISTFNPRLHGVSSCGADQCQFTLGLDMEQTKYRLLTSFGPQNVD
                     QMIDGLYGQAIIPLGNTLSATVGARYAKVTNDIFTGATTPLDDSLSVGSAGLSWRPHA
                     DWRLFARADQNFRFAKVDEHTNPVFGQPVGLNNQTGVSYETGAEFSQGARSAKAVVYE
                     LKLDDEIAFDATGFSNVNLPQTHRRGLMLEGALPLAADWSLQGAYTWTDGKITAGANE
                     GKRIPLVPRSQARLAADWRIAAEWSLRTEGQWTGSQVNGSDFANAFPQLPSYTVANLM
                     LRHEYKDLELGMQVNNLFNHEYSETGAIGLDATFTSRDAYFPAPERNLRLTLGYVWR"
     gene            2593..3198
                     /locus_tag="AC16FV_00985"
     CDS             2593..3198
                     /locus_tag="AC16FV_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR2848670.1"
                     /translation="MALTRFLTISIALHLCLLAGFGHPLLSAPNARLGQSILNIQLQN
                     YERLPAKDQSARPATDVSVAKSSDNYAAVPTETAARSSPADSATGIWAQPSAPTTGTK
                     AGLHNQLLGELRTHLSRYLTYPPLARSHGWEGTVLLSLRVESDGQLDKIRLERSSGYA
                     VLDNSALNSLKRIGNLVEARVWLEGQSIDMQLPVIYKLIDN"
     gene            3205..3756
                     /locus_tag="AC16FV_00990"
     CDS             3205..3756
                     /locus_tag="AC16FV_00990"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR2848671.1"
                     /translation="MGHRLSRITTRTGDDGTTGLGDGSRVKKTSTRIEAIGGVDELNS
                     VIGVLLADEIPQNLQTELTNIQHDLFDLGGELSIPGRSAITDMQVGRLETVLEGLNTK
                     LPPLKEFILPGGSPAAAICHLARTVCRRAERCVIHLGETETINNTSVRYLNRLSDLLF
                     VVARTLNSHAGRADVLWQPGKNR"
     gene            complement(3753..4670)
                     /locus_tag="AC16FV_00995"
     CDS             complement(3753..4670)
                     /locus_tag="AC16FV_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR2848672.1"
                     /translation="MKRLRAIFRCGLLLCLGSVISSPLAVAEIAVVDDAGNTLILLAP
                     ARRVISLAPHVTELLYAAGAGDYVVGAVEYSDYPEPAKRVTRVGSGFGLDLEKIAGLQ
                     PDLVVAWQSGNPSWQIRRLKDLGFPVFETEPHRIGDIAALIERFGKLAATETYARKTV
                     ERFLHDQKNLRDHYANRPSVTVFYQVLDSSLLTINRQHLISDVIQLCGGTNVFADLPG
                     LTSRVDIESVLRKNPEVILASGYEQLWLEWRERWAAWPALSATLHSNLFFIPPDLIHR
                     HTPRILQGAEQICIALEEARAARKTSNVR"
     gene            complement(4702..5313)
                     /locus_tag="AC16FV_01000"
     CDS             complement(4702..5313)
                     /locus_tag="AC16FV_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR2848673.1"
                     /translation="MPYKLTTTLDLMRHGEPVGGRKYRGQLDDPLSEKGWKQMRQAVG
                     ENCPWQVIVTSPLSRCAGFARELAKRHGLPLEADARLIELGFGTWEGCTAEELTAKDP
                     EILARFRSNPLAYTPPGAEPLAVFQKRILEAWNLLHERHSGKHLLIVAHAGVIRVMMA
                     HVLGTPLQHLFRIQVSNAGITRIRVEKIGENLFPQLLFHNGSL"
     gene            complement(5399..6316)
                     /locus_tag="AC16FV_01005"
     CDS             complement(5399..6316)
                     /locus_tag="AC16FV_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobD/CbiB"
                     /protein_id="HIR2848674.1"
                     /translation="MIVILLAILLDHLIPDRHGFKPFVWYRDWAESIEERFNGGKRTH
                     GIGAVMLATLPIVAGVLLIHYILGELGWILRIAFDVVVLYLCLDVHRLGKTANAISNA
                     LESGELGEADEKLRELTGKGATERTEANVARAAVEGVLKQGNSLIVSPIFWFILLGPF
                     GAVLQRLSCILDSLWGHRYERFAEFGWAAARFDDLMGWIPARITALSYALMGSFEDAL
                     HCWRRRVGVWSDINSGPLLASGFGAMHMQICEATEDNEYEERTSDLTVIPDAGHVQRA
                     VALVWRVLLLWLVIGILMTGAHLVGFISG"
     gene            complement(6393..6941)
                     /gene="ampD"
                     /locus_tag="AC16FV_01010"
     CDS             complement(6393..6941)
                     /gene="ampD"
                     /locus_tag="AC16FV_01010"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR2848675.1"
                     /translation="MRIDSGSGLIQGVRFIASPNCDERPAGSPVDTLIIHSISLPPGI
                     FNGPGVEQLFCNCLDPDEHPYYREIKDLKVSAHFLIRRNGELVQFVPLHQRAWHAGQS
                     YCEGRTRINDFSIGVELEGTDNLPFEDAQYASLAKLTDAIRSQYPAITKERIYGHGDI
                     APGRKTDPGPMFDWKRYLGACT"
     gene            complement(7010..7085)
                     /locus_tag="AC16FV_01015"
     tRNA            complement(7010..7085)
                     /locus_tag="AC16FV_01015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7050..7052),aa:Thr,seq:cgt)
     gene            complement(7165..7578)
                     /locus_tag="AC16FV_01020"
     CDS             complement(7165..7578)
                     /locus_tag="AC16FV_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR2848676.1"
                     /translation="MQPKSDTEIFDLLRSHKIHPTSQRVIITRLLFEKCTHLSAEDVF
                     RLVNTDNRHVSKATVYNTLGLLAEKGVVREVIADPARIFYDPNTQPHHHFFDVASGEL
                     TDISVDQIHVSGLPPLPTGARLEGVDVIVRLRPEK"
     gene            7923..8594
                     /locus_tag="AC16FV_01025"
     CDS             7923..8594
                     /locus_tag="AC16FV_01025"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR2848677.1"
                     /translation="MHYHNLKAGSALQNGSPFICRLTPFVGKNNPMMYPFVCLNRPFV
                     RNSGLTLVELIIVLTIVGVLAAFAAPGMQRFVANNRLAAQINDLMADVNLARSEAIKR
                     NTKTTATTTTSTGVCTTATGTSCTAGGNWANGWLVYYNDNGAVTVLKVHETLSGSNTL
                     TATRTDVNTNATTSVDTVSYTNNGALSSMTYTYRFTVCDPNQKQTRMIDITVVGQTSI
                     SSGTC"
     gene            8605..9087
                     /gene="pilV"
                     /locus_tag="AC16FV_01030"
     CDS             8605..9087
                     /gene="pilV"
                     /locus_tag="AC16FV_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HIR2848678.1"
                     /translation="MLSAEKINIGSWNQGFSLIEVLVALLVLSIGLLGLAALQTTSLQ
                     YNTGSYNRTQATFLAYDIIDRMRANSDAVADADGTGYDQPVSANVSSSTNCDTTSCSS
                     PQLALYDVNRWYNRIVATLPDAATTPPTIGIDASKKVTITIRWMERDIRLTQTWTAQL
                     "
     gene            9084..>9263
                     /locus_tag="AC16FV_01035"
     CDS             9084..>9263
                     /locus_tag="AC16FV_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR2848679.1"
                     /translation="MKPYRQHTMGIRGFSLVELMVALTIGLIILAAVSSLFVSSKQTY
                     TTQDRLARLQENARFA"
BASE COUNT         2245 a         2618 c         2405 g         1995 t
ORIGIN      
        1 cctgcgacgg atgcggcaaa acctgctgat gcgacagctc ctgctgcgcc agcagccccg
       61 gcggctcctg ctgctccgga aaagaaataa ggctgaaatt ttttagctgc ctgtttcatg
      121 tttccaagcc ggccttcggg ccggcttttt tattttggtg cgctgaaaaa ataaagattc
      181 acatcgcttt ccttcttttg ttgttattcg tttcgatgtt atccgtcaat cactgaaagt
      241 cattgacacg ttccatcccg cttcatatat tgcgccggag tttgcagttc atcagcttca
      301 ggtgtttccc gaaactgtcg ttacgggaaa tgaaacggga agccggtgcg ttcgtcgaga
      361 ttcaacgacg aatcatcccg gcgctgcccc cgcaacggta aacgagtaag gcgaatcaac
      421 cgccactgtc ccaccttcga ttgcacaggc aatcggcggt gaacgggaag gcgattcgcc
      481 agcatcatcc actcgtgagc ccggagaccg gcctgaagca acccacagga tgtcgcggag
      541 ggcggcccgg gcggtaatgg cctcaccgca ttccgctttc ccttccttcg cacattgctt
      601 atcaaccacc gcgcgggtgc gcgcggtttg cggaggaaaa gtgaaaatgt tttatcgtcg
      661 ttgcatcgtc gcctttgtgg cagtgtattc tcttgccatg tctgttcatg ctgaacccac
      721 ctcccaggat gccatcatag taaccgccac gcgcaatgaa agccccggta tccctatccc
      781 ggccggcatc agcgtgataa gccgtggcga aatcgaagcc agcggcgcgc gctcgctggc
      841 ggaattgttg cgtacacgcg cgggcgtaca ggtctctgat ctgtacgggg acggctccag
      901 cgccaccatt gacatgcgcg gtttcggagc aacggcagtc tccaataccc tggtcttaat
      961 agatggacgc cccctcaaca atgccagtga tatcggcgtc ctggatctat cgctggtgga
     1021 tctgaacaac gtcgagcgag tggagatcgt gcagggcagc gccggtatcc tgtatggaaa
     1081 ccaagcggtg ggtgggctga ttaacatcgt gacacgtcgt gtgccacgcc ggttcgaggc
     1141 agggatcagt gccggttatg gtagctacga tgagcagaga ttgacggcgc aagtgggtca
     1201 gcgtttcgac aatggcttcg ctttcagcct tggcggacgc aaccgcgaca gcgacaatta
     1261 ccgcgatcac aaccacacca agctggatca ggccaggttg cgcctggaac aaactttaag
     1321 cggcggattg gtctttctgg aatacgaaag ctcgagggaa aacgcacaat tgcccggctc
     1381 gctgttcaag gatgagctgg aggcgaatcg ccggcaatct gtcagcgcgt acgccaacga
     1441 ctacaacacg acgcgcaccc aaaccacccg gctcggactt gcacatgcct tgtccgaaaa
     1501 ctggcaattt gagggcgaag caacccatcg ggacaatgac cgtgagttca tcaccggctt
     1561 tcgcttctct cccggtactc tatccaccca acagcgcaag atttccacat tcaatccacg
     1621 cctgcatggc gtatcctcct gcggagcgga ccaatgccag ttcacgctgg gtttggacat
     1681 ggaacaaaca aaatatcgcc tgctaaccag ttttgggccg caaaacgtgg accaaatgat
     1741 cgacggtctg tatggacagg cgatcatccc gctgggaaat acactttcag ctactgtcgg
     1801 cgcacgttac gccaaagtca ccaacgacat ttttaccggc gctacgactc cgctcgacga
     1861 tagtctaagt gtaggttcag cgggactgag ctggcgacct cacgccgatt ggcgactgtt
     1921 cgcgcgtgcg gatcagaatt ttcgcttcgc caaggtggat gaacacacca atcccgtatt
     1981 cggccaaccc gtcggtctca acaaccagac cggcgtttcc tacgagaccg gcgcggaatt
     2041 ttcacaggga gcacggtccg ccaaagcggt ggtgtacgaa ttgaaactgg acgatgaaat
     2101 cgccttcgat gccaccggat tttccaacgt caatctgccg caaacgcacc gccgaggtct
     2161 gatgctggag ggcgccctgc cgctcgcggc cgactggtcg ctgcaaggag cctatacctg
     2221 gactgacggc aagatcaccg ccggagccaa cgaaggaaaa cgcattccgc tggtgccacg
     2281 gtcacaggcc aggctcgcgg cagactggcg catcgccgcg gagtggtcct tgcgcacgga
     2341 aggccagtgg acaggctcgc aggtcaatgg cagcgacttt gcaaacgctt tcccgcaatt
     2401 gccatcctat acggtggcta atctcatgct gagacatgaa tataaagacc tggaactggg
     2461 gatgcaggta aataacctgt tcaatcacga atacagtgag accggcgcca tcgggttgga
     2521 cgcgacgttt acttcacgcg atgcctactt ccctgcgccc gaacgcaatc tgcggttgac
     2581 tctgggctac gtatggcgtt aacacggttc ctgaccatat cgattgcatt gcacctgtgc
     2641 ctgttggcgg gattcggaca tccgctgctg agtgcgccaa atgcccgcct ggggcaatcc
     2701 atactgaata tccaactgca aaattacgag cgcttaccgg caaaggatca gtctgcgagg
     2761 ccagcgacag acgtttcagt tgccaagtca tctgacaatt atgccgcagt gccgaccgaa
     2821 actgccgctc gttcatctcc ggcagattcc gccacaggca tctgggcaca accgtcagca
     2881 ccaacgactg gaacaaaggc gggcttgcac aaccagttgc tgggagaatt aagaacccat
     2941 ctttcacgtt acctgaccta cccgcctctg gcgcgcagcc atgggtggga aggcacggta
     3001 ttactgagcc tgcgcgtgga atccgacggg caactcgaca aaatccgcct ggagcgcagt
     3061 tccggttatg ccgtcctgga caattcggcg ttaaactccc tgaaacggat tggtaatctg
     3121 gttgaagcgc gtgtctggct ggaaggccaa agtatcgaca tgcaattgcc ggtgatttac
     3181 aaattgatcg acaactaaat aaatatgggc caccgtcttt ccaggataac tacccgcacg
     3241 ggcgatgacg gcaccaccgg cctgggcgac ggttcgcgcg tgaaaaaaac gagcacccgc
     3301 atcgaggcca ttggcggggt ggatgaactt aacagtgtca tcggtgtgct gctcgccgat
     3361 gaaattccgc aaaacctcca aacagaactg acaaacatcc agcatgatct tttcgatctc
     3421 ggcggcgagc tcagcatccc gggtcgtagc gctattaccg acatgcaggt cgggcgcctg
     3481 gaaaccgttc tggaagggct gaacacgaaa ctgccgccgc tcaaggaatt cattcttccc
     3541 ggaggatcgc cggcggctgc gatttgtcat ctggcgcgca ccgtgtgccg tcgcgcggaa
     3601 cgttgcgtga ttcaccttgg tgagactgaa acaatcaaca acaccagcgt cagatatctc
     3661 aaccgccttt ctgatctttt attcgtcgtt gcccgcacat taaacagcca tgccggccgg
     3721 gcggatgtcc tgtggcaacc cgggaaaaac cgttagcgaa cgtttgaagt tttacgtgcc
     3781 gcgcgcgcct cttcgagcgc aatgcatatc tgttcagcgc cttgcagaat cctgggagtg
     3841 tggcgatgaa tcaggtcagg aggaatgaaa aacagattac tgtgcagagt cgcggagagc
     3901 gccggccaag cggcccagcg ctcacgccat tccagccata actgctcata accgctggca
     3961 agtatgacct ccggattttt ccgtaacaca gactcgatat ccactcgtga agttagcccc
     4021 ggcaagtcag cgaacacatt ggtcccgcca cataattgaa tgacatcgct gatgagatgt
     4081 tgccggttga tggtcagtaa agaactgtcc agaacctgat aaaacaccgt gactgatggg
     4141 cgattcgcat aatgatcgcg caaatttttc tggtcgtgga gaaatcgttc aactgttttc
     4201 ctggcgtacg tttcggtggc ggccagtttt ccaaatcgtt caatcagagc cgcgatatca
     4261 ccgatacgat gtggttccgt ttcaaaaaca ggaaatccga gatccttcag acgccgtatt
     4321 tgccaggagg gattaccgct ttgccaggcg acaacaagat cgggttgcaa tccggcgatc
     4381 ttttcaagat caagaccgaa cccgctgccc actcttgtca cgcgcttggc cggctcgggg
     4441 taatcgctgt attcaacggc gccgacgaca taatcgcccg ctcctgccgc gtacagcaat
     4501 tccgtaacat gtggtgcaag actgatgaca cgccgcgcgg gggcaaggag aatcaacgtg
     4561 tttcccgcat cgtcgactac ggcaatttcc gccacggcaa gcggagacga tatgacactg
     4621 ccaagacata acagcagtcc acagcggaaa atggcgcgaa gtcttttcat gcgacgcgag
     4681 gacgatcata cctcaacggc gttacaggct tccgttatga aacagtaact gcggaaaaag
     4741 attttcaccg attttctcaa cacgaatacg ggtgataccg gcattcgaca cctggatccg
     4801 gaacaaatgc tgtagcggcg ttcccagcac atgcgccatc atcacgcgga taacgccggc
     4861 gtgcgcaact atcagcagat gctttccgct gtgacgctca tgcaacaagt tccatgcctc
     4921 gagaatgcgc ttctgaaata ccgcaagggg ctcggcgccg ggcggtgtat atgccagcgg
     4981 gttgctgcga aatcgcgcga gtatttccgg gtctttcgcg gtcagttctt ccgccgtaca
     5041 accctcccat gttccaaagc cgagctcgat cagcctggca tctgcctcca gtggcaaccc
     5101 gtggcgcttg gccagctcgc gggcaaaacc ggcgcagcgt gaaagtggtg aagtgacaat
     5161 gacctgccat ggacagtttt ctccaaccgc ctggcgcatt tgcttccagc ccttctcgct
     5221 cagcggatca tccagctgcc cgcgatattt gcgtccaccc acgggttcac cgtgacgcat
     5281 caggtccagc gttgtggtca gtttgtatgg catgaagaag cttgagacaa ggacgttctt
     5341 gttccggcgc gcgattcagt gccctatgtg cgtgcttaac cggagaagac tagatggatc
     5401 agcccgaaat aaagccgacc aaatgggcgc cggtcatcag gatgccgatt accagccaca
     5461 acaacagcac ccgccagacc agcgccacgg cgcgttgcac atgaccggca tccgggataa
     5521 cggtgagatc ggacgttctc tcttcgtact cattgtcctc cgtcgcctca cagatctgca
     5581 tatgcatggc gccaaaaccg gaagccagca aaggaccgct gttgatgtcc gaccacacac
     5641 cgacgcgcct gcgccagcaa tgcagcgcgt cttcaaagct gcccatcagc gcataggaca
     5701 atgccgtgat gcgcgccggt atccacccca ttaagtcatc gaaacgggcg gccgcccaac
     5761 cgaactcggc aaaacgctcg taacgatgtc cccagagcga atcgagaatg caactcagcc
     5821 gttgcaatac ggcgccgaag gggcccagaa gaataaacca gaagatgggc gacacaatca
     5881 gcgaatttcc ctgtttaagg acaccctcaa cggccgcgcg cgcgacattg gcttcagtcc
     5941 tttctgtcgc gcctttcccg gtcaattccc gcagtttctc gtccgcctcg cccaattcac
     6001 cggactccag ggcgtttgat atcgcattcg cggttttacc cagacggtgc acatccagac
     6061 acaaataaag caccacgaca tcaaaagcaa tgcgcaatat ccaaccaagt tcaccgagaa
     6121 tgtaatgaat caacagaaca cccgcgacga ttggaagcgt cgccagcatg acggcaccaa
     6181 taccatgcgt gcgcttcccg ccattgaaac gctcctcgat ggattccgcc caatcccgat
     6241 accagacgaa aggcttgaac ccatgacgat cgggaatcag atgatccagc agaatcgcca
     6301 ggagaattac gatcaatatt tcactcatgc caactgttct tcatcctaat ttgtatataa
     6361 atgtaatggt catagctggc gcaaggcaag ccctaggtgc aggctcccag atatcgtttc
     6421 cagtcgaaca tcggtccagg atcggtcttg cgtccgggcg caatatctcc atgtccgtat
     6481 atacgctctt tcgtgattgc tggatattgc gaccgaattg catcggtaag ctttgccagc
     6541 gatgcatact gggcatcctc aaagggaaga ttgtccgtgc cttcaagttc cacgccgatg
     6601 gagaaatcgt tgatacgcgt gcgcccctcg caatatgact gtccggcatg ccaggcgcgc
     6661 tgatgcagcg gcacaaactg aaccagttcg ccgttccgac gaatgagaaa atgggcggaa
     6721 accttcagat ccttgatttc gcgataatag ggatgttcgt cgggatcaag gcaattgcag
     6781 aacagttgct cgacaccagg tccattgaat atgcccggtg gcaaactgat cgagtgaata
     6841 atcagggtat ccactggaga cccggccgga cgctcatcgc aattgggaga cgcgataaat
     6901 cggactccct gtatcagacc gctgccagaa tcaatccgca tgatcccgcg gtatccctct
     6961 gccgttcgag aaaactccat ggagaaaaga atagctgaca gcccgaaaat ggtgccggtg
     7021 accggaatcg aaccgatgac ctactgatta cgaatcagtt gctctaccaa ctgagctaca
     7081 ccggccttgc gcgatgacag cgggccggaa gtataagctg ttcctctgga tgaaccaacc
     7141 gtagtacggc caggttgctt gagattattt ctcggggcgc aagcgcacga tgacatccac
     7201 cccttcgagt ctggcgcccg tcggaagcgg aggtaaaccg ctcacgtgga tctgatccac
     7261 gctgatgtcg gtcagttcgc ccgaagcgac atcgaagaaa tggtgatgcg gctgtgtatt
     7321 ggggtcataa aagattcgcg cgggatccgc gatcacttcc cttaccacgc ctttctccgc
     7381 cagcaggccg agcgtgttgt ataccgtcgc cttggagaca tgacggttat ccgtatttac
     7441 cagacggaaa acatcctcag cggacaggtg agtgcatttc tcgaacagga ggcgcgtgat
     7501 aattaccctt tgagatgtag ggtgaatctt gtggctgcgc agaagatcaa aaatttctgt
     7561 gtctgattta ggctgcatga tgatacctat ttagaacaat tataaataaa ggtattagcg
     7621 atgtctaaca tacttacaga atgccgaata agaggcatca atactgtttt cagaaaatta
     7681 aatcatcaat atttcagagc agtctcccag acattcaatc ggagattaat accgctcatc
     7741 ggtattaatc taccattcat cccccttatc tctcatgcat ccaagtctgg aaagagattg
     7801 aaaatgggct ttactgacaa ttcgcggcaa ttgcagagtg aaaacccgcg attagactta
     7861 ggtaattgag atcacctcaa tttctgacaa gctctatttt caagcaatat tcgcactgcc
     7921 tcatgcatta ccataatctc aaagcaggaa gcgccctcca gaatggatct cctttcatct
     7981 gccgattaac gccgtttgtc ggtaaaaata atccgatgat gtacccgttt gtctgcctga
     8041 atagaccgtt tgtcagaaat agcggtttaa cattggttga attaataatt gttctaacga
     8101 ttgtaggggt tttggccgca tttgcggcac cgggcatgca gagatttgta gccaacaacc
     8161 ggcttgccgc ccaaatcaat gatctgatgg cggacgtaaa tctggcaaga agtgaggcaa
     8221 ttaaacgcaa taccaagacc acggcaacca caacaaccag tacgggtgtt tgtacaaccg
     8281 ccaccggtac cagctgcaca gccggcggga attgggcaaa cgggtggctt gtttattaca
     8341 acgacaacgg agctgtgacc gttttaaagg tgcatgagac cttgtccggc agtaatacgt
     8401 tgaccgccac acggacggat gtaaacacca acgcgacaac atccgttgac actgtttctt
     8461 acacaaacaa cggtgccctt tcctctatga catacaccta cagatttacc gtctgtgatc
     8521 caaaccagaa gcagactcgg atgatcgaca ttacggtcgt cggccagacg tccatttcca
     8581 gcggaacttg ctgaggatct ttccatgttg tcagcagaaa aaatcaacat cggttcgtgg
     8641 aatcaaggct tctccctgat cgaggttctt gtcgcattgc tcgtgctgtc catcggcctg
     8701 cttggcctag cggcgctgca aacaacctct ctgcaataca acacgggttc gtacaatcgc
     8761 acccaagcca ccttcctggc ttacgacatc atcgatcgca tgcgcgccaa cagcgacgct
     8821 gttgcggacg cggatggcac tggttatgac caaccagttt ccgccaatgt aagcagcagc
     8881 accaactgcg acactaccag ctgctcaagc ccccagttgg cgctctatga cgtcaacagg
     8941 tggtacaaca ggatcgttgc cacgcttcct gacgccgcca ctactccacc gaccatcggc
     9001 atagatgcca gcaagaaagt gacgatcacg attcgctgga tggagcgcga tatacggctg
     9061 actcaaactt ggacagcgca actatgaaac catatcgcca gcacacgatg ggaatccgcg
     9121 ggttcagtct ggtggaactg atggtggcgc tgaccatcgg gcttattatt ctggctgctg
     9181 tttcatcctt gtttgtcagc agcaaacaga cctataccac gcaggatcgt ctggcacggt
     9241 tgcaggaaaa tgcgcgtttt gcc
//
LOCUS       DBNGZE010000040         2493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1534812, whole genome shotgun sequence.
ACCESSION   DBNGZE010000040 DBNGZE010000000
VERSION     DBNGZE010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1534812"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="AC16FV_01040"
     CDS             complement(<1..1219)
                     /locus_tag="AC16FV_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIR2848680.1"
                     /translation="MTDVVATNETILVVGGGISGMTAALEAAECGKDVVLVEKNPSLG
                     GRVSQLYKYFPKLCHPTCGLEINLKRLKANKRIRVLTLAEVKAITGKPGDYTATIKIS
                     PRFVNENCTACGACAEAVTAEFDNEFNYGMGKRKGAYLTSAMAYPQRYVIDPRIIGTP
                     DAEKAKAACKYNAVDLGMKEETINFKVGAVVWATGWKPYDANKIQPYGYDRYPNVITS
                     VEFERLMDPFGPTGGKLIRPSDGKEAKNVAFIQCAGSRDRNHLKHCSRICCMASLKQS
                     TYVREKYADDANVSIYYIDIRAIDRFEDFYKKIQADPKVKFIKSKVANITADKDGNPI
                     LHGVDTEGYHRYDNPHDLVVLAIGMEPSVDASKIMPGVAADASGFIDINATTEAVFGA
                     GCASNALDVNRSVQ"
     gene            1544..2377
                     /locus_tag="AC16FV_01045"
     CDS             1544..2377
                     /locus_tag="AC16FV_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ domain-containing protein"
                     /protein_id="HIR2848681.1"
                     /translation="MSVPKNAASSRVTQVEEYLIKDEPYYEPVGKEIAVFEAAFQQQL
                     PVLLKGPTGCGKTRFMQHMAWRLKRPLITVSCHDDLTATDLVGRYLITANETVWVDGP
                     MARAVRTNAMLYLDEIVEARKDTTVVIHPLADDRRVLPIEKTGELLTAGPEFCLAISY
                     NPGYQSVLKDLKQSTRQRFVAVEFNYPSAALEKKIVRRETGVDEAMVERLVKFAGMTR
                     NLKGSGLDEGASTRLLVHAGKLMSSGIDPRTACRTAIAEALTDDPEMIAAVNELSASL
                     F"
BASE COUNT          528 a          728 c          712 g          525 t
ORIGIN      
        1 tttgcaccga gcggttgacg tcgagcgcgt tggacgcgca accggcaccg aacaccgcct
       61 cggtggtcgc attgatgtca ataaagccgc tggcatccgc cgccacgccc ggcatgatct
      121 tggacgcatc gaccgacggc tccatgccga tcgcgagcac caccaggtca tgcgggttgt
      181 cgtagcggtg atagccttcg gtgtccacgc cgtgcaggat cgggttgccg tccttgtcag
      241 cggtgatgtt ggccaccttg gacttgatga atttcacctt cgggtccgcc tggattttct
      301 tgtagaaatc ctcgaagcgg tcgatggcgc ggatgtcgat gtaataaatc gagacattgg
      361 cgtcgtcggc atatttctcg cgcacataag ttgactgctt gagcgaggcc atgcagcaga
      421 tgcgcgaaca gtgtttcagg tgattgcggt cacgcgagcc ggcgcactgg atgaaggcaa
      481 cgttcttggc ttctttccca tcggagggac gaatcaactt gccgccggtg ggaccgaacg
      541 ggtccatgag gcgctcgaac tcgacgctgg tgatgacatt cggatagcga tcgtagccgt
      601 agggctggat cttgttggcg tcgtacggct tccagccggt ggcccatacc acggcgccga
      661 ccttgaagtt gatggtttct tccttcatgc cgagatcgac cgcgttgtac ttgcatgcgg
      721 ccttggcttt ttcggcatcg ggcgtgccga tgatacgcgg gtcaatcaca tagcgctgcg
      781 gataggccat cgcggaagtg aggtacgcac ccttgcgctt gcccatacca taattgaatt
      841 cgttgtcgaa ttcagccgtg accgcttcag cgcaggcgcc gcaggcggtg cagttctcgt
      901 ttacgaaacg cggggaaatt tttatcgtgg cggtgtaatc gccgggcttg ccggtgatag
      961 ccttgacctc ggccagcgtg agaacgcgga tgcgtttgtt ggccttgagg cgtttgagat
     1021 tgatttccag gccgcaggtg ggatggcaca acttggggaa atatttgtag agctggctca
     1081 cccggccgcc aaggctcggg ttcttttcca ccagcaccac gtctttgccg cattcagcgg
     1141 cttcgagggc cgcggtcatg ccgctgatgc ccccaccgac cactaatata gtctcgttcg
     1201 tcgcgactac atccgtcatt gacatgcctc caccgcggaa ttgatcagaa aataagcacc
     1261 gccggaatta cgggacggca acgatacccc gcaaatgggc ggcgtgctac cggtaaaacc
     1321 gtatcaatag gattgaaatt tcgtatgtgc ggatagaaca cttatataga atgcggcgac
     1381 ccacacacga aacaggcgta caacgcctgc attctcggta agaccgccat tagcgtcaat
     1441 atgtttttct tcctgtaacc gattttggca tactggcccc gtcaacgatt aaccaatatc
     1501 catgaaaacc ttatgacagg acgaacgcag tagggctacc gatatgtcag taccaaaaaa
     1561 tgccgcctcc tcgcgcgtca cccaggtcga ggaatacctc ataaaagatg agccttacta
     1621 tgagcccgtt ggcaaggaga ttgccgtttt cgaagcggcg ttccagcagc aattaccggt
     1681 gctgctgaaa ggtccgaccg gctgcggcaa gacgcgcttc atgcagcaca tggcctggcg
     1741 cctcaagcgc ccgcttatca ccgtgtcgtg tcacgacgat ctcaccgcga ccgatctcgt
     1801 cggccgctat ctcatcaccg ccaacgaaac cgtgtgggtc gatggaccga tggcgcgcgc
     1861 ggtgcgcacc aacgccatgc tctacctcga cgagatcgtc gaggcacgca aggacaccac
     1921 cgtggtgatc catccgctcg ccgacgaccg gcgcgtgctg ccgatcgaga agaccggcga
     1981 gctgctcacc gccggccccg aattctgtct cgccatctcc tacaacccgg gctaccagag
     2041 cgtactgaag gacctgaaac agagtacgcg ccagcgtttc gtcgcggttg aattcaatta
     2101 cccctcggcg gcgctcgaga aaaaaatcgt ccggcgcgaa accggcgtgg acgaggccat
     2161 ggtcgagcgg ctggtgaaat tcgccggcat gacacgtaac ctgaaaggca gcggcctcga
     2221 cgaaggcgcg agtacgcgcc tgctggtgca cgccggcaag ctcatgagtt cgggcatcga
     2281 cccgcgcacc gcctgccgca ccgccatcgc ggaagctcta accgacgacc ccgagatgat
     2341 cgcggcggtc aacgaactca gcgcatcgtt attttaataa ttttcgccgc agagacgcag
     2401 agaacgcaga gttaagaaaa gaacaactcg aagttcataa aacttaattc ttcctggttt
     2461 tttctctgcc ctctgcgtct ctgcggcaag tga
//
LOCUS       DBNGZE010000041         3147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_701123, whole genome shotgun sequence.
ACCESSION   DBNGZE010000041 DBNGZE010000000
VERSION     DBNGZE010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_701123"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16FV_01050"
     CDS             complement(<1..233)
                     /locus_tag="AC16FV_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848682.1"
                     /translation="MSIKVVTGNHAAGHALSAAGEANRHARGVVCGIYPITPQTEIVE
                     HVANFHFTKGHVVAVESEHSAMGVCIGAALNGAR"
     gene            complement(239..2446)
                     /locus_tag="AC16FV_01055"
     CDS             complement(239..2446)
                     /locus_tag="AC16FV_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR2848683.1"
                     /translation="MTEIRIHGRGGQGGVTLAKIVAQARHIEGKSVQAFGLYAAERSG
                     APLQAFLRYDDRPIDNRNLIYEPDHIIVLDPTLVSPTIVAGLKPQAFILINSPEPPQT
                     FGRQEALSPYRIATVDATSIARAHKLGTRSLPIVNSALAGAAERLLEMPIDSLLEALR
                     EMGFSGQNAKAAREAYDSVRVLEQKAAPSRNQKVTVPTTLIPGIIAGNTGAPPTIRTG
                     QWASQQPVRQDSVPPCNFSCPAGNNVQGFLDALAKDDIDRALSVLLQTTPFPSVCGRV
                     CPGFCMQVCNRASFDEPVNVRALERFAGDHGQVTLTCQDERPQAVAVVGSGPAGLSGA
                     YHLALLGYRVTVFESDSELGGLLRTGIPSYRLPREALDRDIGRILALGVQVVTNHPVD
                     RAKLEQLRKSYDAVLVATGLQKLTNVDLAQDDGTVIQALDFLNKARKGEIDTLDGQVV
                     VVGGGNTAIDSARSAWRLGAKSVTVVYRRTRAEMPAISEEVDEAIDEGISFEFLLSPM
                     KLQLRNGSHVLVCQRMELGPPDPDGRRKPVPVPGEVIEMACDRVLLALGQYADLSLLP
                     DGTRLAGEHGPAPVGHGIVPAGDLLTNEGTVTAAIGCGREMALRLHGEWTGQPIRPPK
                     ASPEEVIRADRIRFHLFTHSMKNQEHATPVDERRHTFTEVHRGLDSFEEARRCLSCGV
                     CNACDLCLSACPDGVVLRVNGELLFDYDYCKGCGVCVSECPRAVIYMKTVGQE"
BASE COUNT          720 a          879 c          836 g          712 t
ORIGIN      
        1 cgcgctccgt tcagcgcggc gccaatgcag acgcccatgg cactgtgttc actctcgacc
       61 gcgacaacat gccccttggt aaagtggaaa ttcgccacgt gttcaacgat ttcagtttgc
      121 ggtgtaatcg ggtagattcc acaaacgact ccccgtgcgt ggcggttggc ttcgccggcg
      181 gcgcttaggg catggccggc cgcatgattc cccgtgacta cttttatcga catccgactt
      241 actcctgtcc aaccgttttc atgtagatga cggcgcgcgg acattcggac acgcacacgc
      301 cgcatccttt gcagtagtca taatcaaaca gcagttctcc attcactcgt agcaccaccc
      361 catccggaca agcactaaga cagaggtcgc aggcattgca aactccacag ctcagacagc
      421 gacgcgcttc ttcgaagctg tccaggcccc ggtgcacttc cgtaaaggtg tgccgacgtt
      481 catccacggg agttgcatgt tcttgatttt tcatggaatg agtgaaaagg tggaatcgga
      541 tccggtcggc ccggatgact tcttccgggc tcgctttggg cggccggatc ggttgacccg
      601 tccattctcc gtgcaagcgg agcgccatct cgcggccgca gcctatggcc gcggttacgg
      661 ttccttcgtt ggtcagtaga tcgccggcgg gaacaatgcc gtggccaacc ggtgcaggcc
      721 cgtgctcgcc ggccagtctg gttccgtcag gcagaagtga gagatcggca tattgaccca
      781 atgccaggag tacccgatcg cacgccatct cgatcacttc gccaggcaca ggcaccggtt
      841 ttcgacgccc gtctggatcg ggcggtccca attccatccg ctggcaaacc agtacatgtg
      901 aaccgttgcg caattgaagc ttcatcgggg aaagtaagaa ttcaaatgag attccctcgt
      961 cgatagcttc atccacttct tcagagatag cgggcatttc agctcgcgtg cgccgataaa
     1021 caacagtcac gctcttggct ccgagacgcc aggccgaacg ggccgagtcg atggcggtat
     1081 tgccgccacc cacaacaacc acctgtccat ccaaggtatc aatttcaccc ttgcgagctt
     1141 tgttcaagaa atccaatgcc tgtattactg ttccgtcgtc ttgggccaga tcgacattcg
     1201 tcagcttctg caaccccgtc gccaccagaa ctgcatcata tgattttcgc aattgctcga
     1261 gctttgcccg atcgaccgga tgattggtga cgacttgaac gcccagagca agaatgcgac
     1321 cgatgtctct atccaaggct tctcggggaa gtcgatacga gggtattcct gttcgcaaaa
     1381 gaccgccgag ttcagaatcg ctttcaaaca ctgtcacccg gtagcccaaa agagccagat
     1441 gataggcacc cgacagaccg gccggaccgg accccaccac cgccaccgct tgaggccgct
     1501 cgtcctgaca tgtgagggta acctggccat gatctcctgc aaaccgctcc agcgcgcgca
     1561 cattaacagg ttcatcgaag ctggcgcggt tacatacctg catgcaaaaa ccggggcaaa
     1621 cccgcccgca caccgacggg aaaggggtcg tctgcaacag aaccgataag gcgcggtcaa
     1681 tgtcatcttt ggctaaggca tcaaggaatc cctggacgtt gttcccagcc gggcaactga
     1741 aattgcaggg cggtacggaa tcttgtcgca caggttgctg gctggcccac tggccggttc
     1801 ggatagtcgg aggagcaccg gtattcccag caataatacc cggaatcaat gtagtgggca
     1861 ctgtgacttt ctgattgcga gagggagcgg ccttctgttc caacactcga acagagtcat
     1921 aagcttcgcg cgcggcctta gcattctgcc cgctgaaccc catctcgcga agagcttcaa
     1981 gaagagagtc aataggcatt tccagcaacc gctccgccgc accggcaagg gcagagttga
     2041 cgataggcag actgcgagtc cccagtttat gggcgcgggc aattgacgtg gcgtcaacgg
     2101 ttgcgatccg gtatggagaa agtgcttctt gccggccgaa tgtctgaggc ggttccggcg
     2161 aattgatcag gataaacgcc tggggtttga gtccggcgac tatcgtgggc gaaacaagcg
     2221 tcggatccag gacaataata tgatcgggtt cgtaaatgag gtttcgattg tctatggggc
     2281 ggtcatcgta tcggaggaaa gcttgaagcg gggcgccgga tcgctcggct gcatacagac
     2341 cgaaggcctg caccgatttc ccctcaatgt ggcgcgcttg tgccacaatt tttgccagcg
     2401 ttacccctcc ctggccgcct ctgccatgta ttctgatttc agtcatcagc gacatctcca
     2461 gttaagcgaa gcagattcgg acggtggcgc acacccgcca tctaaaacaa tttcggtcgc
     2521 ggatggagcc ataatttcca tctctcaagg tcttcctaac ggaccggctt atatgattta
     2581 atatatcact tttttttgta aatctgccac attcaaagac atatcatttg tcggcagata
     2641 gattacaggc gcgaggaacc ccgtatatca gcccaatgtc ctttcgattt gccggtaact
     2701 attctaagta catcagcgaa taatgctcta cctttgcgga cgctaagtcg cgggggaaat
     2761 cgataccctg tgctgatttt aatgatgaat tgagatgcac ttggtgaaag atcaacatgt
     2821 ttatgagaaa aatgccgtgc cccacttgag atttggacag gtccaattgt gtaaggagga
     2881 ttaatgaaga acgcttcatc agggggattt aggcaggtat caataccctc aaattttaag
     2941 cactttttcg atgaattgga cggaggggtt cacaaatcgt ttggtcgggc atccgctatg
     3001 gacaaatttc gcaccggatg gttcgcacat cgtttaactc caactacccc gctatgaaca
     3061 ggacgacaac cagtaactga ttgtcgccct tcgattttgg tagcgggggt tggatttgaa
     3121 ccaacgacct tcgggttatg agcccga
//
LOCUS       DBNGZE010000042         5570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_493070, whole genome shotgun sequence.
ACCESSION   DBNGZE010000042 DBNGZE010000000
VERSION     DBNGZE010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_493070"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..636)
                     /locus_tag="AC16FV_01060"
     CDS             complement(100..636)
                     /locus_tag="AC16FV_01060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR2848684.1"
                     /translation="MAEKQTLARPYAEAVFELAQARSALKLWSEMLRLIAAIAADENM
                     QRLAADPRADRARFRELFLDVCGKNLDDAGANFVRLLVENRRLSLLPEIVAQYETLKA
                     EAEARVEATVTSAFALEPEQMKSLGEALKRKLGREVNLTAQVDKALMGGIVIRAGDLV
                     IDGSVRGRLADLATHMST"
     gene            complement(649..1119)
                     /locus_tag="AC16FV_01065"
     CDS             complement(649..1119)
                     /locus_tag="AC16FV_01065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR2848685.1"
                     /translation="MNINATLIGQSIAFFLFVWFCMKFIWPPLTRALDERKKTIADGL
                     AAAEKGKRELELGEKKALETIKQAKQDASEVIALAEKRAAEIADEAKENAKAEAERIV
                     AAARADIEQEVNRAKESLRAAVSELAVVGAARILEKEVDAKAHARLLEAVVKQL"
     gene            complement(1156..1392)
                     /gene="atpE"
                     /locus_tag="AC16FV_01070"
     CDS             complement(1156..1392)
                     /gene="atpE"
                     /locus_tag="AC16FV_01070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR2848686.1"
                     /translation="MEQSLLYIAGALMMGLGALGAAVGIGILGGRFLEGVARQPELIP
                     TLRTQFFIVMGLVDAVPMIAVGISMYILFAVVGG"
     gene            complement(1435..2277)
                     /gene="atpB"
                     /locus_tag="AC16FV_01075"
     CDS             complement(1435..2277)
                     /gene="atpB"
                     /locus_tag="AC16FV_01075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR2848687.1"
                     /translation="MTGETLTSSEYIKHHLQNLTFGRLPDGTWGLAHSAEQAKEMGFW
                     AMHLDTMAFSLGLGILFLWLFRKAAKRATSGVPGGLQNFVEWIVEFIDGTVRGSFSGR
                     NPMVAPLALTIFIWVFLMNFMDLLSIDHLPQVAHATGVPFLKAVPTTDPNATFGMSLA
                     VFALILYYSIKVKGIGGFLGELTLQPFGKWGLPVNLFLEGVNLIAKPVSLALRLFGNM
                     YAGEMIFILIAVMYGASWLLGSFAGLLQLGWAIFHILIITLQAFIFMTLTIVYLDMAH
                     QEHH"
     gene            complement(2329..2679)
                     /locus_tag="AC16FV_01080"
     CDS             complement(2329..2679)
                     /locus_tag="AC16FV_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR2848688.1"
                     /translation="MSFAAEKLRKGVRRVLLFQALLTLLVAAAFGVARGRYVFLSALY
                     GGAVAVLLSGWLGRGVLRSTGLGSLYANAITRYGAAMLFLGLGMGVLKLAPLPLIVAF
                     AVAQFGFLAEMRST"
     gene            complement(2861..3301)
                     /locus_tag="AC16FV_01085"
     CDS             complement(2861..3301)
                     /locus_tag="AC16FV_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848689.1"
                     /translation="MKPAIILLILGLTAPLLVGAQDLSSLEQMRVAAEKGSADAQLEM
                     GILYEFGYNMPKNHVTALAWYLRAAEQGNTLAAKRRDLIQSGMKPDEIEAAQKLSQEL
                     AAQKSQAAAEPPAAEPAPAPAESAPAPEAPLAGPPTVDTPASKP"
     gene            complement(3411..3487)
                     /locus_tag="AC16FV_01090"
     ncRNA           complement(3411..3487)
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_01090"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            complement(3548..4402)
                     /locus_tag="AC16FV_01095"
     CDS             complement(3548..4402)
                     /locus_tag="AC16FV_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR2848690.1"
                     /translation="MTTKKPQRLGRGLDALLSAYEAPVEKDELRQIPIDQLQRGKYQP
                     RTHMNPEALEELANSIKAQGVVQPIVARPLSAGSYEIIAGERRWRAAQLAGLETIPAV
                     VRKIPDEAAIAIALIENIQRENLNPVEEANAFTRLIDEFHMTHQQVAEAVGRSRAAVS
                     NLLRLLTLQADVRELLETGKMDMGHARALLALEGGAQSKAAHQVVDRGLSVRETENLV
                     RRLLAGPAGHKSSGHRMDPDTRALAQRLSEKLGAKVRFQHSAKGKGRLLIEYNTLEEL
                     DGILAHIR"
     gene            complement(4419..5195)
                     /locus_tag="AC16FV_01100"
     CDS             complement(4419..5195)
                     /locus_tag="AC16FV_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR2848691.1"
                     /translation="MGKVIAITNQKGGVGKTTTSINLAASLAKTRRRVMLIDIDPQGN
                     ATMGSGVNKQANRVTTYEVLIGLEPIEAARVTVEGGYDLVPANSELSGAEVELIEIER
                     RERRLRDAIEKVRAQYDYILIDCPPALNLLTVNALVAAQAVIIPMQCEYYALEGLTAL
                     LNTIRKIRDTLNPQLEIEGLLRTMYDPRNNLDNEVSAQLKQHFGDKLYRTVIPRNIRL
                     AEATSHGKPVIAYDMQSKGAQAYLALAGEMLRREEEVVAA"
     gene            complement(5217..>5570)
                     /locus_tag="AC16FV_01105"
     CDS             complement(5217..>5570)
                     /locus_tag="AC16FV_01105"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR2848692.1"
                     /translation="DLEFTLLDSNAKKTRFAAQALHELGLKNVVVVQERVEKFHPAEK
                     FDTLIARAFASIPDMLAASRHLCTPGGRFLVMKGVFPQEELAAVTDGYRAEVKALTVP
                     GLDAARHMVILAPVN"
BASE COUNT         1029 a         1747 c         1706 g         1088 t
ORIGIN      
        1 agttttcgcc gcagagacgc gaaggacgca aagaaaagca ttttaaattt taaaatcttt
       61 gcgctctttg cgcctttgcg gcagataatc ataaattcat caggtactca tgtgggtggc
      121 gaggtcggcg agccgtccgc gcaccgagcc gtcgatcacg aggtcgccgg cgcggatcac
      181 gatcccgccc atcagcgcct tgtccacctg cgcggtgaga ttcacttcac gtcccagctt
      241 gcgcttgagc gcttcgccga gagatttcat ttgttccggt tccagcgcaa acgcggaggt
      301 cacggtcgcc tccacgcgcg cctcggcctc ggccttgagc gtttcatact gcgccacgat
      361 ctcgggcagc aaactcaggc ggcggttctc caccagcagg cggacaaagt tcgcgcccgc
      421 gtcatccaga tttttcccgc acacgtccag aaacagttcg cgaaaacgcg cgcgatcggc
      481 gcgcggatcg gccgcgaggc gctgcatgtt ttcatcggcc gcgatcgcgg cgatgaggcg
      541 caacatctcg gaccatagtt tcagcgcgct gcgtgcctgc gccaattcga agacggcttc
      601 ggcgtagggg cgggcgagtg tctgtttttc agccattttt gcgggccatt acagctgctt
      661 gaccacagcc tcgagcagcc tggcgtgggc cttggcgtcc acttccttct cgaggatgcg
      721 cgcggcgccg accacggcca attcggatac cgcggcccgc agcgattcct tggcgcggtt
      781 cacttcctgc tcgatgtcgg cgcgcgccgc ggcgacgatg cgctcggcct cggccttggc
      841 gttttccttg gcctcgtctg cgatctcggc ggcgcgtttc tcggccagcg cgatgacttc
      901 ggacgcgtcc tgcttggcct gcttgatggt ctcgagcgcc ttcttctcac ccagctccag
      961 ttcgcgcttg cccttctcgg cggcggccag gccgtcggcg atggtcttct tgcgctcgtc
     1021 cagcgcacgc gtcagcggcg gccagatgaa cttcatgcag aaccacacga acagaaagaa
     1081 cgcgatggac tgcccgatca aggtggcatt gatattcatg tcctgtcccc cgaaatcaga
     1141 aaatatgcgt caggcttagc cgccgacgac tgcgaacagg atgtacatgg agataccgac
     1201 agcgatcatc ggcaccgcgt ccaccagacc catgacgatg aagaactggg tgcgcagcgt
     1261 cggaatcagt tccggctggc gcgccacgcc ttcgagaaag cggccaccca ggatgccgat
     1321 gccgacggcg gcgccaagcg cgccgagacc catcatcagg gcgccggcga tatacagcag
     1381 ggattgttcc attgttttct cctatcgaaa tttcagaaat gttgtgaaat cggttcagtg
     1441 atgttcctga tgcgccatgt cgagatagac gatggtgagc gtcatgaaga tgaacgcttg
     1501 cagcgtaatg atcaggatgt gaaagatggc ccagccgagc tgcagcaggc ccgcgaacga
     1561 gccgagcagc cagctcgcgc cgtacatgac ggcaatcaat atgaagatca tttcaccggc
     1621 gtacatgttg ccgaacagtc gcagtgccag cgataccggc ttggcgatga ggttcacgcc
     1681 ctcgagaaac aggttgaccg gcaggcccca cttgccgaac ggctgcaacg tgagctcgcc
     1741 gagaaaaccg ccgatgccct tgaccttgat gctgtagtag agaatcagcg cgaacaccgc
     1801 gagcgacatg ccgaaggtgg cattggggtc ggtggtcggc acggctttca ggaacggcac
     1861 gcccgtagcg tgggcgacct ggggcaagtg atcgatggac aacagatcca taaagttcat
     1921 caggaaaacc cagatgaaga tggtgagcgc cagcggcgcg accatgggat tgcgtccgct
     1981 gaaggaacca cgcacggtgc cgtcgatgaa ttccacgatc cactcaacga aattctgcaa
     2041 gccgcccggg acgcccgatg tggcgcgctt cgccgccttg cggaacagcc acaggaacag
     2101 aataccgagg ccaagggaga aagccatggt atcgaggtgc atcgcccaga agcccatttc
     2161 cttcgcctgc tcggcgctgt gggcgagtcc ccacgtgcca tcgggcagac ggccgaaggt
     2221 cagattctgg aggtgatgct tgatgtattc tgacgaggtc agcgtttcgc ccgtcatggt
     2281 ttagtcagtc ccgccaatca aaacttgttc atccggtttt caggcaactc aggtgctgcg
     2341 catctccgcc agaaacccga actgggccac ggcaaaggcc acgatcagtg gcaacggcgc
     2401 cagcttgagc acgcccatcc ccagacccag aaacaacatc gccgcgccat agcgcgtgat
     2461 cgcattcgcg tacagcgagc cgagaccagt ggagcgcaag acgccgcgac ccagccatcc
     2521 gctcaacagc accgcgaccg cgccgccata caacgccgat aaaaaaacgt accggccgcg
     2581 agcaacgccg aaggcggcgg caacgagcag cgtcaacaag gcttggaaca ataagacccg
     2641 gcgcacccct ttgcgcagtt tttctgccgc aaagctcatg tcagcgccac ggattgcgtg
     2701 ttttaacctt gaaaacggtg gaaaaacggc tttttcaaag gcggcgaagt ataaagacag
     2761 ccccccggag ggtcaatata gcccgataga ttccggctat tgccggaatg ccacgggaaa
     2821 tcaaaatgag gccaaccgta actgttgcga gccggtcagc tcagggcttg gaggcgggtg
     2881 tgtctacggt cggagggccg gccagcggtg cttccggtgc cggcgccgat tcggctggcg
     2941 cgggcgccgg ctcagccgcg ggaggctcag ccgcggcttg tgatttttgc gcggccagct
     3001 cctgcgacag cttctgcgcc gcttcaatct cgtccggctt catgccggac tggatcaggt
     3061 cgcggcgctt ggccgccagc gtgttgccct gttccgcggc acgcagatac cacgccagcg
     3121 cggtgacatg attcttcggc atgttgtagc cgaactcgta gaggatgccc atttccagct
     3181 gggcgtcggc gctgcctttc tccgccgcga cgcgcatctg ctcgagcgat gacaaatcct
     3241 gtgctcccac gagcaggggt gcggtcaagc cgaggatcaa aaggatgatt gccggtttca
     3301 tcaggcctcc gttgccttcc tgaaaacttt ctctgttcta gttctagtta tagacggcga
     3361 ttcaagggga tagcaagtgg cgctggccgg ggttccgacg actagaagcc atctcaaaac
     3421 cgtcgcgagc ggccgtcagc cgggtgagga cggagcgcag gaaccggagt gtacacggta
     3481 agtacatgag gattccgagc accgcccgat cccggatcac ggacgcgcag caggttttga
     3541 gatggcttca gcggatatgg gccagaatcc cgtccaattc ttccaaggtg ttgtactcga
     3601 tcagcagccg gcccttgccc ttggcgctgt gctggaaacg caccttggcg cccagctttt
     3661 ccgaaagtcg ctgcgccagt gcgcgggtgt cggggtccat gcggtggccc gaggatttgt
     3721 gtccggcggg acccgccagc aggcggcgca cgagattttc cgtttcgcgc accgacaacc
     3781 ccctgtccac cacttggtgc gcggccttgc tctgggcgcc gccctcgagc gcgagcaggg
     3841 cgcgcgcgtg gcccatgtcc atctttccgg tttcgagcag ctcgcgcacg tcggcttgca
     3901 gcgtgagcag acgcagcagg ttgctcaccg ccgcgcgcga acggcccacg gcctcggcca
     3961 cctgctggtg cgtcatgtgg aattcatcga tcaggcgcgt gaaggcgttg gcctcctcga
     4021 ccgggttcag gttctcgcgc tggatgttct cgatcagcgc gatcgcaatc gcggcctcgt
     4081 ccggaatctt gcgcaccacc gccgggatag tttccaggcc ggccaactgc gccgcacgcc
     4141 agcgacgttc gccggcgatg atctcgtagc tgccggcgga cagcgggcgc gccacgatcg
     4201 gctgcaccac gccctgggcc ttgatggaat tggcgagctc ttccagcgcc tcgggattca
     4261 tgtgcgtgcg cggctggtac ttgccgcgct gcaactggtc gatcgggatc tggcgcaact
     4321 cgtccttttc gaccggcgcc tcgtaggcgc tcaacagggc gtcgagcccg cgcccgaggc
     4381 gctggggttt cttggtggtc atcacgctca tcgcctcatt atgctgccac aacctcttcc
     4441 tcgcggcgca acatctcgcc cgccagcgcc agataggcct gcgcgccctt ggactgcatg
     4501 tcgtaggcga tcaccggctt gccgtgcgac gtcgcctcgg cgagccggat gttgcgcgga
     4561 atgacggtgc ggtaaagctt gtcgccgaag tgctgtttca attgcgccga aacttcgttg
     4621 tcgagattgt tacgcgggtc gtacatggta cgcagcagac cttcgatctc cagttgcggg
     4681 ttcagcgtgt cgcgtatctt gcggatggtg ttgagcagcg cggtgagccc ttcgagcgcg
     4741 tagtactcgc actgcatcgg gatgatcacc gcttgtgccg ccaccagcgc attgaccgtc
     4801 agcaggttca gcgctggcgg acaatcgatc aggatgtaat catattgcgc gcgcaccttc
     4861 tcgatcgcgt cgcgcaaacg ccgctcgcgc cgctcgatct cgatcagctc cacttccgcg
     4921 ccggacagtt cgctgttggc gggcaccaga tcgtaaccgc cttccaccgt cacgcgcgcg
     4981 gcctcgatcg gttccaggcc gatcaggact tcatacgtcg tgacgcgatt ggcctgcttg
     5041 ttgacgccgc tgcccatggt ggcgttgccc tgcgggtcga tgtcgatcag catcacgcgc
     5101 cggcgcgtct tggccagcga ggcggcgaga ttgatgctgg tcgtggtctt gccgaccccg
     5161 cctttctgat tggtgatggc tatgaccttg cccatcgggc agctgctcct tccgcgtcaa
     5221 ttcactggcg ccaggatgac catgtgtcgc gcggcatcca ggcccgggac tgtcagcgcc
     5281 ttcacttcgg cccggtatcc gtccgtgacc gcggccagct cttcctgggg aaacacaccc
     5341 ttcatgacca ggaaccggcc gcccggcgtg cacaggtggc gactcgcggc cagcatatca
     5401 ggaatggacg cgaacgcgcg agcaatcagc gtatcgaatt tttccgccgg atggaatttc
     5461 tccacgcgct cctgcaccac gactacattt ttcaggccca gttcgtgcag cgcctgcgcg
     5521 gcgaagcggg ttttcttggc gttgctgtcg agcagggtga attcaagatc
//
LOCUS       DBNGZE010000043         4141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1187549, whole genome shotgun sequence.
ACCESSION   DBNGZE010000043 DBNGZE010000000
VERSION     DBNGZE010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1187549"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..455)
                     /locus_tag="AC16FV_01110"
     CDS             complement(201..455)
                     /locus_tag="AC16FV_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR2848693.1"
                     /translation="MSLLITDECINCDVCEPECPNGAITQGEEIYQINPDLCTECVGH
                     YETSQCVDVCPVDCIIPDPNHAETRDQLLLKYQALILKTA"
     gene            complement(494..973)
                     /gene="coaD"
                     /locus_tag="AC16FV_01115"
     CDS             complement(494..973)
                     /gene="coaD"
                     /locus_tag="AC16FV_01115"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR2848694.1"
                     /translation="MKVVALYPGTFDPITNGHTDLVRRAARLFDHVIIAVAANPQKKP
                     LFNLTERVELARKVLADVKGVSVTGFDNLLVDCVRQHKANVILRGLRAASDFEYEFQL
                     AGMNRRLAPEVETVFMTPSEQEMFISASLVKEIALLGGDVAQFVDARVAAAMAAKIR"
     gene            complement(1050..1694)
                     /gene="rsmD"
                     /locus_tag="AC16FV_01120"
     CDS             complement(1050..1694)
                     /gene="rsmD"
                     /locus_tag="AC16FV_01120"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR2848695.1"
                     /translation="MARAERKRARRSPAAPVSAPKSSSAKPKSSYPHKLRIIGGRWRG
                     RRLDVAESEGLRPTPDRVRETLFNWLQPYIAGASCLDLFAGTGALCLESLSRGAADVV
                     MVEKMPSVAQRLRQHVERLEATGAEVVLADAVDFLQRTPRAFDIVFLDPPFKSNLIAD
                     CAALVEARGWIKPGGLIYVEVPSQMTELALPATWELIRSKKAGQVGYHLARKTA"
     gene            complement(1681..2985)
                     /locus_tag="AC16FV_01125"
     CDS             complement(1681..2985)
                     /locus_tag="AC16FV_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2848696.1"
                     /translation="MFARILLLIGLLLASFNALAGPKIQHWTLSNGARVYFVESRELP
                     MLQVRVVFDAGSSRDPAEKAGVANLTVTMLDEGADGLSTDEIASRFEGVGAEFSVGVD
                     RDMATVNLRSLSDSALLDPALELFARILAKPAFPVENLERQRAQALVALQKDAQSPGA
                     IAEKAFYRELYGQHPYASDPLGNETSLKAITREDLLEFHRRHYVGANAWVVIVGDASK
                     RQARNIAERIIGKLPAGKAPSPLPPVRTLDSGWMKQINFPATQTHINMGQPGVRRGDP
                     DYFPLYVGNYILGGGGLVSRLSVEVREKHGLSYSVYSYFYPLRMPGPLFIGLQTKNTQ
                     RDEALKLVRQVLTDFVAKGPTDQELEAAKKHITGSFPLRLDSNAKIAENLAVIGFYGL
                     PLTYLDDFIPRVEAVTVEQIRNAFRRRVHPDQMVTVTVGGAR"
     gene            complement(2975..>4141)
                     /locus_tag="AC16FV_01130"
     CDS             complement(2975..>4141)
                     /locus_tag="AC16FV_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2848697.1"
                     /translation="MFKGTAKHKPNEFSRIIAENGGRENAFTSYDYTAYFQQLEKSRL
                     PIAFELEADRMQNLVLTEEEFGKEIKVVMEERRLRTEDQPEALVSEKFMSTAYEVHPY
                     KHPVIGWMADLQSMTVKDVRDWYGRYYTPTNALLVVTGDVEPKEVFALARKYFGPVPA
                     RSPVTTAAAAEPPQKSEHRTRVALPAEVPYLLLGYHTPVHASAGRNEIAEWEPYALSI
                     LGGVLDGGQAARFERELVREKKIASSIDLDYSAVSRSPSMLLIEATPANGHTTAELEK
                     AVRDQIGRIQNEPVSAEELKRVKAQVVAGNVYSRDSVFYQAMQIGRLAAVGLDWLLLD
                     DYVKNLNAVTAEQVQTVARKYLVDTNLTVAVLDPQPMAKGKPRSAPRLGGGHVR"
BASE COUNT          813 a         1329 c         1201 g          798 t
ORIGIN      
        1 ccgtggcagg cgggcgcggt tccactgccc agcctgccac aggcaacatc gccagcaaga
       61 tgagtaacaa gcgcaaatat cgatgcatgt ctgacaagat aatggaatcg gagtggcgag
      121 gcaaaaactg ttggagtttc gtattgcgaa taaaaaaggc ggctcatggc cgcctttttt
      181 tcaaaccgga aaatttccga tcatgccgtc ttcaggatca aggcctgata tttcaatagc
      241 aactgatctc tggtttccgc gtgattcgga tcggggatga tgcagtccac cgggcagaca
      301 tccacgcact gggaggtctc gtagtgaccg acacattcgg tgcagagatc gggattgatc
      361 tgataaatct cctcgccctg cgtgatcgcg ccgttagggc actcgggctc gcacacgtcg
      421 cagttgatgc actcgtcggt aatcagtaaa gacataaaat acctgcaaaa tatccggtta
      481 aagcgttgtc atcttaacgt attttggccg ccatcgcagc cgccacgcgc gcgtccacga
      541 attgcgcgac gtcgccgccc aacagggcga tctccttcac cagcgaagcc gagatgaaca
      601 tctcctgctc cgacggcgtc atgaacaccg tttccacttc cggcgccaag cgccggttca
      661 tgcccgcgag ctggaactcg tattcgaaat ccgacgccgc ccgcaaaccg cgcaggatca
      721 cattggcctt gtgctgccgc acgcaatcga ccagcagatt gtcgaagccg gtgacgctca
      781 cgcccttgac gtcggccagc accttgcgcg ccagttccac gcgttccgtc agattgaaca
      841 gcggcttctt ctgcggattc gccgccaccg cgatgatcac gtggtcgaac aggcgcgcgg
      901 cgcggcgcac cagatccgtg tggccgttgg tgatcgggtc aaacgtgccg ggatacagcg
      961 cgacaacttt cattcagccc ctcggaacgg aacaaagcct atcattgcca atagccgcct
     1021 cattgacgga aatcaagaac accctgcgct catgcggtct ttcgcgccag atgatacccc
     1081 acctgcccgg cctttttgct gcgaatcaac tcccacgtcg ccggcagcgc cagttcagtc
     1141 atctggctcg gcacctcgac ataaatcagc ccgccgggct tgatccaccc gcgcgcctcc
     1201 accagtgcgg cacagtccgc gatgagattg cttttgaagg gcggatcgag aaagacaata
     1261 tcgaacgccc gtggtgtgcg ttgcagaaaa tccacggcat cggccagcac cacctcggct
     1321 cccgtggcct ccagccgctc cacatgctgg cgcagccgct gcgccacgga cggcatcttt
     1381 tcgaccatca ccacgtccgc cgcgccgcgc gagagcgact ccaggcacag cgcgccggtg
     1441 ccggcgaaca gatcgagaca actcgctccg gcgatataag gttgcaacca gttgaacaac
     1501 gtttcgcgca cacgatcggg cgtggggcgc agaccttccg attccgcaac gtccagacgc
     1561 cggccgcgcc agcgtccacc gatgatgcgc agcttatgtg gataggatga ttttggtttc
     1621 gcggaagatg atttcggcgc ggatacgggc gcagccggcg agcggcgcgc gcgtttccgc
     1681 tcagcgcgcg ccaccgacgg tcaccgttac catctggtcg ggatgcacgc gccgccggaa
     1741 ggcattccgg atttgttcga cggtcacagc ctcgacgcgc ggaatgaagt catcgagata
     1801 agtgagcggc aatccataga aaccgatcac cgccaggttc tcggcgatct tggcgttgct
     1861 gtcgagtcgc agggggaagc tgccggtgat gtgtttcttc gcggcttcca gctcctgatc
     1921 cgtcggaccc ttggcgacaa aatccgtcag cacctggcgc accagcttga gcgcctcatc
     1981 acgctgtgtg tttttcgttt gcaggccgat gaacagcggt ccgggcatgc gcagcggata
     2041 aaaataactg taaacgctgt aggacaggcc gtgcttctcg cgtacctcca ccgacaggcg
     2101 cgacaccagc ccgccgccgc cgagaatata gttgcccacg tagagcggga aataatccgg
     2161 atcgccgcgc cgcacccccg gctgtcccat gttgatgtgt gtctgggtgg cgggaaaatt
     2221 tatctgcttc atccagcccg agtcgagcgt gcgcacgggc ggcaaaggag atggcgcctt
     2281 gccggcaggc agcttgccga tgatgcgttc agcgatattc cgcgcctgac gcttgctggc
     2341 gtcgccgacg atcaccaccc aggcgttggc gccgacataa tggcgccggt ggaactccag
     2401 caggtcctcg cgcgtgatcg ccttcaggct cgtctcattc ccgagcggat cgctggcata
     2461 agggtgctgc ccatacagtt cacgataaaa cgccttttcg gcgatcgccc ccggcgattg
     2521 cgcgtccttt tgcagggcca ccagggcctg cgcccgctgg cgttccaagt tctccacggg
     2581 gaacgcgggc ttggccagaa tccgggcgaa cagttcgagt gccggatcca gcagggcgga
     2641 atccgacagg ctgcgcagat ttacggtcgc catatcgcga tcgacaccga cactgaattc
     2701 ggcacccacg ccctcaaaac ggctggcgat ctcgtccgtg ctcaggccgt ccgcgccttc
     2761 gtccagcatg gtgaccgtca gattggccac gcccgccttt tccgcgggat cgcggctgga
     2821 accggcgtcg aacaccacgc gcacctgcag catgggaagt tcgcggctct cgacgaaata
     2881 gacgcgcgcg ccgttggaca aggtccagtg ctggatcttg gggcctgcca gggcattgaa
     2941 cgacgccagt aaaagaccga tcaacagcaa aattctagcg aacatggccg cctcccaggc
     3001 gcggggcgga acgcggcttg cccttcgcca tcggctgcgg gtcgagcacg gccaccgtga
     3061 gattcgtatc gaccagatat ttgcgcgcaa ccgtctgcac ctgttccgcg gtcacggcat
     3121 tgagattttt cacgtagtca tcaagcagaa gccagtcgag gccgaccgcc gccaggcgcc
     3181 cgatctgcat cgcctgatag aacaccgaat cgcgcgaata gacattgccc gccaccacct
     3241 gcgccttcac gcgtttcagc tcctcggccg acaccggctc gttctggatg cgtccgatct
     3301 ggtcgcggac ggccttttcc agctccgcgg tcgtgtggcc attggccggt gtggcctcga
     3361 tgagcagcat ggatggagaa cgcgaaaccg ctgaataatc caaatcgatg ctggaagcga
     3421 ttttcttctc gcgcaccagc tcgcgctcga agcgcgccgc ctgcccgccg tcgagcacac
     3481 cgccgagaat gctcagcgca tatggctccc actcggcgat ttcattacgg ccggccgacg
     3541 catgcaccgg cgtgtgatag cccagcaaca gatacggcac ctccgccggc aacgcgacgc
     3601 gtgtccggtg ctcgcttttt tgcggcggtt ccgcggctgc ggcagtcgtc accggtgagc
     3661 gcgcgggaac cggcccgaaa tatttccggg ccaacgcgaa aacttccttg ggctccacat
     3721 cccccgtcac gaccagaagg gcattagtgg gcgtgtaata gcggccgtac cagtcgcgca
     3781 catccttgac cgtcatggat tgcaggtccg ccatccagcc gatgaccgga tgcttgtacg
     3841 gatgcacttc ataggcggtg ctcatgaact tttcgctgac cagcgcctcg ggctgatcct
     3901 cggtgcgcaa ccggcgctcc tccatcacca ccttgatctc cttgccgaac tcctcctcgg
     3961 tcagcaccag attctgcatg cgatcggcct cgagctcgaa ggcgatgggg agacgcgatt
     4021 tctccagttg ctggaaatac gcggtgtagt cgtaactggt gaaggcgttt tcacgcccgc
     4081 cattctcggc gatgatgcgt gaaaactcgt tgggtttgtg cttggccgtg cccttgaaca
     4141 t
//
LOCUS       DBNGZE010000044         2125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1257038, whole genome shotgun sequence.
ACCESSION   DBNGZE010000044 DBNGZE010000000
VERSION     DBNGZE010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1257038"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="AC16FV_01135"
     CDS             complement(<1..742)
                     /locus_tag="AC16FV_01135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR2848698.1"
                     /translation="MLQTLLQNETAIRLTAFFGVLALVAGAEFIFPRRRLTVSKLRRW
                     ASNLGIVFLNSALVRVLLPITAAGFAVLAASRGWGLFNNFPVPVWFAVLASVVILDFA
                     IYLQHVMFHAVPALWRLHRMHHADLDFDVTTGARFHPIEIILSMLIKFAAIAVIGAPA
                     AAVIIFEVLLNATSMFNHGNLRLPGVLDRVLRLFIVTPDMHRVHHSIEDHETNSNFGF
                     NLPLWDRLFGTYKDQPDAGHEAMTIGIRD"
     gene            852..1541
                     /locus_tag="AC16FV_01140"
     CDS             852..1541
                     /locus_tag="AC16FV_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2848699.1"
                     /translation="MSRQNLKQQIYSQVARIGKVVCHGHRLELLEYLAQGSRTVEALA
                     KLTGLSVANTSQHLRVMRHSGLVQARKDGLYVYYSLADDEVVRLLSSMRKLAESHLAD
                     VDRLVNAYLTVKDELEPIPRRELLERARKGIVTVLDVRPPEEDAAGHVPGAVNVPLKD
                     LAKRLKELPRGQEIVAYCRGPHCVLAFEAVAQLREKGFQARRMEDGFPEWREAGLPVE
                     KSTKATADERR"
     gene            complement(1646..>2125)
                     /locus_tag="AC16FV_01145"
     CDS             complement(1646..>2125)
                     /locus_tag="AC16FV_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR2848700.1"
                     /translation="VAPTTNSYKRLVPGYEAPVNLAYSSRNRSAAVRIPMYTPSPKAK
                     RIEVRFPDPSCNPYLGFAAMLMAGLDGIQNKIDPGEPLDKNIYDLPPEELAKVPSCPA
                     SLEAALDALDRDRAFLMQGDVFTADVIDTWLDYKRKHECDAIRMRPHPYEFMLYYDA"
BASE COUNT          428 a          608 c          698 g          391 t
ORIGIN      
        1 agtcgcggat gccgatggtc atggcttcgt gtccggcgtc gggttgatcc ttgtaagttc
       61 caaacaggcg atcccagagc gggaggttga agccgaagtt gctgttggtc tcgtggtcct
      121 cgatcgaatg gtgcacgcga tgcatgtcgg gtgtgacgat gaacagccgc aacacgcgat
      181 cgagcacgcc cggcaggcgc aggttgccgt gattgaacat ggaagtggcg ttgagcaata
      241 cctcgaaaat gatcaccgcc gccgccggcg cgccgataac ggcgatggcc gcgaacttga
      301 tgagcatgga caggatgatt tcgattggat ggaagcgcgc gccggtggtg acatcgaaat
      361 cgagatcggc gtggtgcatg cggtgcagcc gccacagcgc cggtaccgcg tggaacatca
      421 cgtgctgcag gtagatggcg aaatcgagaa tgacgaccga ggcgaggacg gcgaaccaga
      481 ccggaacagg aaaattgttg aacaggcccc agccgcggct cgccgccagc accgcgaagc
      541 ccgccgccgt tatgggcagc agcacgcgca ccagcgcgct gttcaggaac acgatgccga
      601 gattactcgc ccagcgacgc agtttggaaa cggtcaaccg gcgcctgggg aatatgaact
      661 cggccccggc cacgagggcc agcaccccga agaaagccgt cagacgtatc gccgtttcgt
      721 tttgcagcag tgtttgtagc atggggtccc cgcgtatccg ttggttgtca gtcttataaa
      781 ttgcttgaat taataggcgc gtgtgctagt attcaattac ttaattgaat actagattat
      841 agaactcgga catgtcaaga cagaacctga aacagcagat ctacagccag gtggcgcgca
      901 tcggaaaggt ggtctgtcac ggccaccggc tcgaattgct ggagtacctg gcgcagggca
      961 gccgcacggt cgaggcgctg gccaagctca ccggcctgtc ggtggcgaac acctcgcagc
     1021 acctgcgggt catgcgccac agcgggctgg tgcaggcgcg caaggacggc ctgtatgtct
     1081 attacagtct ggccgacgac gaagtcgtcc ggttgctctc cagcatgcgc aagctcgccg
     1141 aatcgcacct ggccgacgtg gaccgtctcg tgaacgccta cctcaccgtc aaggatgaac
     1201 tggaaccgat cccgcgacgc gagctgctcg aacgcgcgcg caagggaatc gtcacggtgc
     1261 tcgatgtccg cccgccggag gaagatgccg ctgggcacgt tcccggcgcc gtgaacgttc
     1321 cgctgaaaga tctggcgaag cggctcaagg aattgccgcg gggccaggag atcgtcgcct
     1381 actgtcgcgg accgcactgc gtgctcgcct tcgaggccgt ggcacagttg cgcgaaaagg
     1441 gattccaggc gcgccggatg gaggatggtt ttccggagtg gcgcgaggcc ggcctgccgg
     1501 tcgagaagag cactaaagca accgcagatg aacgcagatg agtcaaaaat gatgaacgca
     1561 gattagatgg agacgtttca actcatgttg atctgcgtcc atccgcgctt ctatccgcgt
     1621 ccgtctgcgg tccgctcttt tttcgctacg cgtcgtagta cagcatgaac tcatacgggt
     1681 gcggacgcat gcgtatcgcg tcgcactcgt gtttgcgctt gtaatccagc caagtgtcga
     1741 tcacgtcagc ggtgaacacg tcgccctgca tcaggaatgc gcggtcgcgg tcgagcgcat
     1801 cgagtgctgc ctcgagcgag gctgggcagc tcggcacctt cgccagctcc tcgggcggca
     1861 ggtcatagat gttcttgtcg agcggctcgc ccgggtcgat cttgttctgg atgccgtcga
     1921 gcccggccat gagcatggcg gcgaagccga gatagggatt gcacgacgga tcggggaaac
     1981 gcacctcgat gcgcttggcc ttgggggatg gcgtgtacat cgggatgcgc accgcggccg
     2041 agcggttgcg cgacgagtag gccagattca ccggcgcttc gtaccccggc accaggcgct
     2101 tgtacgagtt ggtcgtgggc gccac
//
LOCUS       DBNGZE010000045         4250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1465814, whole genome shotgun sequence.
ACCESSION   DBNGZE010000045 DBNGZE010000000
VERSION     DBNGZE010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1465814"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="AC16FV_01150"
     CDS             complement(<1..798)
                     /locus_tag="AC16FV_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR2848701.1"
                     /translation="MPSAAPDPLYLCLDQGGHASRALVFDRFGVLQASVLREVAVREP
                     QTGWVEQDPEELVASIQAVITKTVVSLGARAGQIVSAGIATQRASMVCWDRHTGEALS
                     PIISWQDRRASAWLDQFSAHTHEVHQHTGLMLSPHYGASKMRWCLDHLPAVAAARREA
                     RLAMGPLASFLLFRLLEEKPFFADPANAARTLLWNLRALDWDPWLLELFGIPPETLPR
                     CVPTHHAFGTLRANEHRLPLTIATGDQSAALFALGMPPADIGYVNIGT"
     gene            complement(805..1614)
                     /gene="cysQ"
                     /locus_tag="AC16FV_01155"
     CDS             complement(805..1614)
                     /gene="cysQ"
                     /locus_tag="AC16FV_01155"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR2848702.1"
                     /translation="MNDFNPRDQLNGVCDIARDAGRVILDVYERGFTVEQKEDRSPLT
                     EADRAAHEIICARLEALTPGVPVLSEESAKVDPETRAGWKRFWLVDPLDGTKEFINRN
                     GEFTVNIALIDGHRPVLGVVYVPVTGLTYFAAAGHGAYKQKGECEVQVIKTRRFNGGK
                     PIVVASRSHAGPETEAFLKNLGAHDVVSMGSAIKLCLVAEGTADVYPRLGPTMEWDTA
                     AAQCVVEAAGGRVTDLNRQPLAYNKFSLLNPSFMAIGAGDYDWYRHLPSLK"
     gene            complement(1621..2169)
                     /gene="nudE"
                     /locus_tag="AC16FV_01160"
     CDS             complement(1621..2169)
                     /gene="nudE"
                     /locus_tag="AC16FV_01160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018937093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HIR2848703.1"
                     /translation="MSRKPVITRTETIARTRLFRIEQVGLRFANGCEVNYERLRSASG
                     GAVLVVPMLDDSTVLLIREYAAGVERYELALPKGLVEEGESPFEAANREIMEEVGYGA
                     RKLTHLKSVTLAPGYFSHATHLILAQELYPERRPGDEPEAIEVVPWSLDRLTELFEHE
                     ECTEARSIAALLLVRELLAGRR"
     gene            2301..2648
                     /locus_tag="AC16FV_01165"
     CDS             2301..2648
                     /locus_tag="AC16FV_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIAA family protein"
                     /protein_id="HIR2848704.1"
                     /translation="MIVIEEQNDLLKATVYAELSLADFREFETAVQHGLKQTPKIKLL
                     LDLTKMSGYTVDVAWEDIRFTRAHAHDFRRIAVVTGSMWVTWLGWLPSTFTDAEIRHF
                     EDAAAANAWLQGR"
     gene            2668..3318
                     /gene="yrfG"
                     /locus_tag="AC16FV_01170"
     CDS             2668..3318
                     /gene="yrfG"
                     /locus_tag="AC16FV_01170"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HIR2848705.1"
                     /translation="MIAWNQLDTLLLDLDGTLLDLHYDNHFWLEHVPRRYAEVHGLPP
                     EAAREEVLARYKRAEGTLNWYCVDYWSRELRLDIPQLKEEVNHLIAVHPHVLDFLEAV
                     KALKKRVVLVTNAHGKTLAINFQRTALHGHFDAVVCAHDLGLPKEHADFWDKMQQVQP
                     FEKRTALLIDDSLPVLRSASQYGIGQLLAVYKPDSKLPEKDVGEFRAIRSFREIMP"
     gene            3334..3900
                     /locus_tag="AC16FV_01175"
     CDS             3334..3900
                     /locus_tag="AC16FV_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848706.1"
                     /translation="MKPAVIPLLAVLLVACQTSAYEGNENSPFYSVPAGSTLTLTRSL
                     TVPANQVAVFLQGGEVVASGAINQYYPHCKFELQHRRDMAQTVRADSFQITKVVQEIG
                     HSVALDGLQLAGVSVGIGINIGSRGDGASLQTWSTRMTLRSTRQPEVFRLSCGQAALP
                     HEGQHVSINEMRKALGNVFTLDLTKKNR"
     gene            3923..>4250
                     /locus_tag="AC16FV_01180"
     CDS             3923..>4250
                     /locus_tag="AC16FV_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase family protein"
                     /protein_id="HIR2848707.1"
                     /translation="MNTEFLGAALESPFKMLMLLVIANGAPILGKKIFGTAFSHRLDG
                     GIKLRDGHPLFGPSKTFRGLVLSIVATALVAIPLGYDWIDGALISVLSMLGDLTSSFV
                     KRRLGRP"
BASE COUNT          829 a         1354 c         1283 g          784 t
ORIGIN      
        1 ggtcccgatg ttgacgtagc caatatcggc tggcggcatg cccagcgcga acaacgccgc
       61 ggattgatcg ccggtggcga tggtcagcgg caggcgatgt tcattcgcgc gcagcgtgcc
      121 gaaagcatga tgcgtgggca cacaacgcgg cagcgtctcc ggcggtatcc cgaaaagttc
      181 cagtaaccac gggtcccaat ccagcgcgcg aaggttccac agcagcgtgc gcgcggcatt
      241 ggcaggatcg gcaaaaaacg gtttttcctc aagcagacgg aacagcagaa agctcgctag
      301 cgggcccatg gcgaggcgcg cctcgcgccg ggcagcagcc acggccggca agtggtcgag
      361 acaccagcgc attttggaag cgccgtaatg cggtgaaagc atcagcccgg tgtgctgatg
      421 aacttcgtga gtgtgtgcgg agaattggtc cagccatgcc gacgcgcgcc ggtcctgcca
      481 gctgatgatg ggtgagagcg cctcacccgt atgacggtcc cagcagacca tgctcgctcg
      541 ttgcgtggcg ataccggcgc tgacaatttg cccggcgcga gcacccagcg agacaacagt
      601 tttggtaatt accgcttgaa ttgaagcgac cagctcctcc ggatcctgct ccacccagcc
      661 cgtctgcggt tcacgcaccg ctacttcgcg caggacgctg gcctgcaaga cgccaaaacg
      721 gtcgaacacc agcgcgcgac tggcgtgacc gccctgatcg agacagagat agagcggatc
      781 aggcgcggcg gatggcaaac gcgtttattt caagctgggg agatgacgat accagtcgta
      841 atcaccggca ccgatcgcca tgaacgaggg attcagcagc gagaatttgt tgtacgccag
      901 cggttggcga ttgaggtcgg tcacgcgccc gccggcggcc tcgaccacgc actgcgccgc
      961 cgccgtgtcc cactccatgg tcggaccgag gcgcggatag acgtcggccg tgccctccgc
     1021 caccagacac agtttgatgg cgctgcccat ggacacgaca tcgtgcgcac cgaggttctt
     1081 gagaaaggcc tcggtttccg ggccggcgtg ggaacggctc gcgaccacga tcggcttgcc
     1141 gccattgaaa cggcgcgtct tgatgacctg cacctcgcac tcaccctttt gcttgtacgc
     1201 accgtgaccg gcggcggcga aataggtaag ccccgttacc ggcacataca ccacacccag
     1261 caccggccgg tgaccatcga tcagggcgat gttcacggtg aattcgccgt tgcggttgat
     1321 gaattccttg gtgccgtcca gcgggtccac cagccagaat cgcttccagc ccgcgcgcgt
     1381 ttctggatcg accttggcgg attcttcgga gagcacgggc actccggggg tgagcgcctc
     1441 cagtctggcg cagatgattt catgcgcggc gcggtccgcc tcggtgagcg gggagcggtc
     1501 ttccttctgt tccacggtga atccgcgctc gtagacatcc agaatgacgc gaccggcgtc
     1561 gcgcgcaatg tcgcaaactc cattcagttg atcacgggga ttgaaatcat tcattgacgg
     1621 tcagcgcctc ccggccagta gttcgcgcac cagcagcagc gccgcgatcg agcgcgcctc
     1681 ggtgcattct tcatgttcaa aaagttccgt caggcggtcc agcgaccacg gcacgacctc
     1741 gatcgcttcc ggctcgtcgc cgggccttcg ttcggggtaa agctcctgcg ccagaatcag
     1801 atgggtggca tgactgaaat aaccgggcgc gagcgtgacc gacttgaggt gagtcagctt
     1861 gcgcgccccg taaccgacct cctccatgat ctcgcggttg gcggcctcga acggcgattc
     1921 gccttcctcg acgagcccct tgggcagcgc cagctcgtaa cgctcgacgc ccgcggcgta
     1981 ctcgcggatc agcagcaccg tgctgtcgtc cagcatcggc accaccagca cggcgccgcc
     2041 ggacgcgctg cgcagacgct cgtagttcac ctcgcacccg ttggcgaagc gcagccccac
     2101 ctgctcgatc cggaacaggc gcgtgcgcgc gatggtttca gtgcgggtga taacgggctt
     2161 ccggctcatg ccaactccgt gagacatatt tccaaggtta atcctttttg attggcgaaa
     2221 caaaattcat tcattccctc tgcccccgca acaggtatat gcttgtgccg atacattttt
     2281 aatacgtccg gagccaaccc atgatcgtaa tcgaggaaca gaacgacctg ctcaaagcga
     2341 cggtctacgc cgaactgtcg ctcgcggatt tccgggaatt tgaaaccgcc gtccagcatg
     2401 ggctcaagca gacgccgaaa atcaaactgc tgctcgacct gacgaagatg tcgggctaca
     2461 ccgtcgacgt cgcctgggag gacatccgct tcacccgcgc ccacgcccac gatttccgcc
     2521 gcatcgcggt ggttaccggc agcatgtggg tgacctggct cggttggttg ccctccacct
     2581 ttaccgacgc cgagatcaga catttcgagg acgccgcggc ggcgaatgcc tggctgcaag
     2641 gccgttgata gctgtgttct cccacccatg atcgcctgga accagctcga caccctgctg
     2701 ctcgatctcg acggcacgtt gctcgacctg cattacgaca atcacttctg gctggagcac
     2761 gtgccgcgcc gctacgcgga ggtgcacgga ctgcctcccg aggccgcccg ggaggaagtg
     2821 ctggcgcgct acaagcgcgc cgagggcacg ctcaactggt actgcgtgga ctactggagc
     2881 cgggaattgc ggctcgacat cccgcagctc aaggaagagg tgaatcacct gatcgcggtg
     2941 catccgcatg tgctcgattt cctcgaagcg gtaaaggcct tgaaaaaacg cgtggtgctg
     3001 gtcaccaacg cccacggcaa gacgctggcc atcaacttcc agcgcaccgc cctgcacggc
     3061 catttcgacg ccgtggtgtg cgcgcacgac ctagggctgc cgaaggagca cgcggatttc
     3121 tgggacaaga tgcagcaggt gcagccgttc gagaagcgca ccgcgctgct catcgacgac
     3181 agcctgccgg tgttgcgctc ggccagccag tacggcatcg gtcagctgct tgcggtgtac
     3241 aaaccggatt cgaaactgcc ggaaaaggac gtgggggaat tccgcgccat tcgcagtttc
     3301 cgggagatca tgccctgagg caaaggaggc aacatgaaac cggccgtcat tccgctactt
     3361 gcggtgctgc tcgtggcctg tcagacatcg gcctatgaag gcaatgagaa ctcgccgttt
     3421 tattccgttc ccgccggctc aacgcttacg ctcacacgca gtctgaccgt tccggcgaat
     3481 caggtggcgg tcttcctgca aggcggcgag gtcgtggcgt cgggggcaat caaccagtat
     3541 tacccgcatt gcaaattcga gctgcaacac cgtcgcgaca tggcgcagac cgtgcgggcg
     3601 gacagtttcc agatcaccaa agtcgtgcag gaaatcggac acagcgtcgc gctcgacggc
     3661 ttgcaactcg caggcgtatc ggtcggcatc ggcatcaaca tcggttcgcg cggcgacggc
     3721 gccagcctcc aaacctggag cacacggatg acgttgcgct ccacgcgcca gccggaggtg
     3781 ttccgcctgt cctgcgggca ggcggcgctg ccacacgagg gtcagcacgt cagcatcaac
     3841 gagatgcgca aggcgctggg gaatgtattt acgctcgact tgacgaagaa aaacaggtag
     3901 ctctcgcaat tccaaggact gaatgaacac cgagttcctc ggcgccgccc tcgaatcgcc
     3961 gtttaaaatg ctgatgctgt tggttatcgc caacggtgcg ccaatcctgg gcaaaaaaat
     4021 atttggtacg gcattcagcc accggctcga tggcggcatc aaactccggg atggccatcc
     4081 gctgttcggc ccttccaaga ccttccgggg tctcgtcttg tccatcgtcg ccacggcact
     4141 cgtcgctatc ccgctcggtt acgactggat tgacggtgcc ctgatttcgg tcctgtctat
     4201 gttgggggac ctcacctcaa gcttcgtcaa acgccgcctc ggccggccgc
//
LOCUS       DBNGZE010000046         4852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_76852, whole genome shotgun sequence.
ACCESSION   DBNGZE010000046 DBNGZE010000000
VERSION     DBNGZE010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_76852"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="AC16FV_01185"
     CDS             complement(<1..642)
                     /locus_tag="AC16FV_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2848708.1"
                     /translation="MPPAAARPSARYRSVIVVLILAFILLGFFALGRLPIDLPPSGDA
                     LRLNIRASAPGLTAPVIEAMLTRRLETLLTGVPGVVVMESVTTTGNVSVDLLLNHRRD
                     IDAAQREVTSRLERDKASWPASIDPPSMTLTDTASASVEFNLTSRTLDALALRDWVEA
                     EFARRLRELSGIAAVDVQGGSVREILVMPDQRRLAGYGLSFEDLLQAIRKNPEV"
     gene            complement(656..1144)
                     /locus_tag="AC16FV_01190"
     CDS             complement(656..1144)
                     /locus_tag="AC16FV_01190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR2848709.1"
                     /translation="MIDERGYRPNVGIVLCNRMNQVFWARRCGQDGWQFPQGGIRPKE
                     PVEQAMYRELREEVGLSPVHIEVLGRTRDWLHYDIPDHYRRRHPHSAFRGKKQIWYLL
                     RLIGQDRDVRLDACDRPEFDEWRWIEYWSALEHIIGFKRQVYQQALTELEPLLSSVKT
                     LG"
     gene            1191..1556
                     /locus_tag="AC16FV_01195"
     CDS             1191..1556
                     /locus_tag="AC16FV_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848710.1"
                     /translation="MIFPRSRDGGNAALDRWRPVGIALTLVGLALTGCASTPSIPANV
                     ILRDVHNNGYALAFDNASKTFASGGSEGRIRLWTLPEGKEAAGWTAHNGSLQGLHFLD
                     HEREILSAGYDGALARWTR"
     gene            1563..2213
                     /locus_tag="AC16FV_01200"
     CDS             1563..2213
                     /locus_tag="AC16FV_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR2848711.1"
                     /translation="MLKRLDTPAPITDMDADETTALVVTGHRDGHVRLWRLTDFALLH
                     DLPLHRGAVRAVTYHPATRQLASSGTDGRVFYWRLGEQPHPLPSPPTDAQTLAFAPDG
                     GILMGSGWFNLFRWRLTDASLTVLPTEHHGIVKSISFEHDGRRLVSISRQTDSSVYLL
                     DAETGKVVRRFQPHELCGTAVRLSPDSRYLASTSDDANIHLWDLRHLLPEQTFYSK"
     gene            2384..3037
                     /locus_tag="AC16FV_01205"
     CDS             2384..3037
                     /locus_tag="AC16FV_01205"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR2848712.1"
                     /translation="MSLAIFDLDNTLLRGDSDHAWGRFLIENHIVDGEEYERENERYY
                     AQYQAGTLDIMEFLAFALRPLALHDRATLDGWHRQYMQTKVLPMITPAARALVNLHRA
                     HGDTLVIITATNRFVTAPIADEFDIPHLIATEPEEHNGRFTGKVAGTPCYREGKVTRL
                     QSWIQEHHQTLQDSWFYSDSHNDLPLLSIVDHPVAVNPDEILDREARKRGWQILPLR"
     gene            3050..4021
                     /locus_tag="AC16FV_01210"
     CDS             3050..4021
                     /locus_tag="AC16FV_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR2848713.1"
                     /translation="MNRRLLTMLILLALAPLSLWFALSQGSLNIVAPELWQALRGNSD
                     NATTYLVVHELRLPRALAAFAVGGLLALSGALLQVLLRNPLADPYVLGVSGGAAVAAL
                     LSMLGGLGIGWVRGNAFLGALISMFIVFGLSRLGAVWTQNRLLLTGVVIAAGWGALIS
                     LVLALAPMAQVQGMLYWLIGDLSYATRPAVALLALLLGLVVSLWFARALNLLMRGENT
                     AAALGENPARLRFIIYLIASLLTAMAVTLAGSVGFVGLVIPHILRLLAGSDHRFLLPA
                     CVLLGGGFLTIADTLARSLAAPVQLPVGVITALLGIPVFLYLLIRSR"
     gene            4021..4782
                     /locus_tag="AC16FV_01215"
     CDS             4021..4782
                     /locus_tag="AC16FV_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848714.1"
                     /translation="MLRARNLSLTIAGRTLCADLNVNLQAGENWVILGANGSGKTTLL
                     HALAGLRAPNAGGVSLDNREIGAIPPRARARRLGVLFQETESEFPATVIETVLTGRYP
                     HLDRWQWEGAQDYHMAEAALAAVGLDGLAWRPLATLSGGERRRVEIAALLAQDAPICL
                     LDEPTNHLDLRHQVQVLNLLAGRAQQPGYLNVFVLHDVNLAARFGTHGILLFDNGECR
                     HGPLSDLLDRPHLERLYHCRLREIRDGGSVWFLPE"
BASE COUNT          900 a         1527 c         1437 g          988 t
ORIGIN      
        1 cacctcggga tttttccgga ttgcctgcaa cagatcctcg aacgaaagac catatccggc
       61 cagccgtcgc tgatccggca tgaccaggat ttcgcgcacg ctgccgccct gaacatccac
      121 cgcggcgatg ccggacaatt cccgcagccg tctggcaaac tcggcctcga cccagtcgcg
      181 cagcgcgagc gcatcgagtg ttctggatgt cagattgaat tccacgctgg ctgacgcggt
      241 atccgtcagc gtcatcgatg gcggatcgat ggaggccggc caggaggcct tgtcccgttc
      301 cagacgcgac gtcacctcgc gttgcgcggc gtcgatgtcg cgccggtgat tcagcagcag
      361 atcgacgctg acattccccg tggtggtaac cgactccatg acgaccacgc ccggcacgcc
      421 ggtgagcagg gtttccagac gccgtgtcag cattgcttca atcaccggcg cggtcagccc
      481 cggcgcggag gcgcggatat tcagacgcaa tgcatcgcct gatggcggaa gatcgatggg
      541 caggcggccc aacgcaaaaa aacccagcaa tatgaatgcc aggatgagaa cgacgataac
      601 ggaacgataa cgggcggagg gacgtgctgc ggctggtggc atgcgccgcg cgtcttcagc
      661 cgagggtttt tacggatgac aacaacggtt cgagctcggt cagggcctgc tgatagacct
      721 ggcgcttgaa accgatgata tgttcgagcg cgctccagta ttcgatccag cgccattcat
      781 cgaattcggg ccggtcgcac gcatcgagac gcacgtcccg atcctgtccg atcaggcgca
      841 gcaaatacca gatctgcttc ttgccgcgga aggcactgtg cggatggcgg cggcggtaat
      901 gatcggggat gtcgtaatgc agccagtcgc gggtgcggcc cagcacttcg atatgcacgg
      961 gtgacaggcc cacttcctcg cgcagttcac ggtacatggc ctgttcgacc ggttctttgg
     1021 ggcggatacc gccctgcgga aactgccagc catcctgacc gcaacgccgc gcccagaaaa
     1081 cctggttcat gcggttgcac agaacaatac ccacgttggg gcggtagccg cgttcgtcta
     1141 tcattacaat cccgccaacc gttcaagaac aagcgtatcg gaacaataac atgatttttc
     1201 cacgaagccg cgatggcggc aatgcggcat tggaccggtg gcggcctgtc ggtatcgcct
     1261 tgacgctggt cgggctggcg ctgaccggat gcgcgtccac gcccagcata cccgccaatg
     1321 tcatcctgcg ggatgtccac aacaacggct atgcgctggc cttcgacaat gcgtcgaaga
     1381 cattcgcctc gggcggctcg gagggacgca tacgcctgtg gaccttgccg gaaggcaagg
     1441 aagccgccgg ctggacggcg cacaacggct cgcttcaagg actgcacttc ctcgatcacg
     1501 aacgcgaaat attgtccgcc ggatacgacg gcgcgctcgc gcgctggaca cgctaagggg
     1561 ctttgctcaa acgtctcgac acacccgcgc ccatcaccga catggacgcg gacgaaacca
     1621 cggcgctcgt ggtcacgggg caccgtgacg ggcatgtacg cctgtggcgc ctgaccgatt
     1681 tcgctctcct gcacgatttg ccgcttcatc gcggcgcggt gcgcgccgta acttatcacc
     1741 ccgctacccg gcaactggct tcaagcggta cggatgggcg cgtcttttat tggcgtctgg
     1801 gggagcaacc gcatccgctc ccctctcctc cgacggacgc acaaactctg gcctttgcgc
     1861 ccgacggcgg tattctcatg ggcagtggct ggttcaatct attccgctgg cggctaacgg
     1921 atgcatcgct caccgttctg ccaacggaac atcacggcat cgtcaaatcc atcagcttcg
     1981 aacatgacgg tcgccgcctc gtcagcatca gccgacaaac tgacagctcg gtgtatttgc
     2041 tggatgcaga aaccggaaag gtggtcaggc gctttcagcc gcatgaactg tgcggaactg
     2101 ccgtgcgtct ttcacccgac agccgttatc ttgcctcgac ttccgacgac gcgaatatcc
     2161 atctgtggga cttgcggcat ctcttgccgg aacagacttt ctattcaaaa taaaatatat
     2221 ttcaccgcca agacgccaag aaaacggcat taacaggatt tacaggatta aaacaggatt
     2281 aacaggattt ttcttaaaaa ttctgattct ttctggaatc tttaatcctg taaatcctga
     2341 gcaatcctgt taatcctgtc tatttaattt gggactttca accttgagcc ttgctatctt
     2401 cgacctcgac aacacgctgc tgcgcggcga cagcgaccat gcctggggtc gctttctcat
     2461 cgaaaatcac atcgtggacg gcgaggaata cgaacgcgaa aacgaacgtt attacgcgca
     2521 gtatcaggct ggcacgctcg acatcatgga gtttctggcc ttcgccctgc gcccgctcgc
     2581 gcttcacgac cgcgccactc tcgacggctg gcaccggcaa tacatgcaaa ccaaggtttt
     2641 gcccatgatt acacccgccg cacgtgcgct cgtgaacttg catcgcgcgc acggcgacac
     2701 gctggtcatc atcactgcga ccaaccgctt cgtcaccgca ccgattgccg atgagtttga
     2761 catcccacac ttgatcgcga ccgagccgga agaacataac ggccgcttca cgggaaaggt
     2821 cgcgggaaca ccgtgctatc gtgaaggcaa agtcacacgc ctccagagct ggatacagga
     2881 gcaccatcag acactgcaag acagctggtt ttacagcgat tcgcacaacg atctgccgct
     2941 gctttccatc gtcgatcatc cggtggcggt caatcccgac gagattctgg accgcgaagc
     3001 gcgcaaacgc ggctggcaga ttctgccact gcgctgaaca agactcgcca tgaaccgccg
     3061 cctgctgacg atgctgattt tgctggcgct cgcgcctcta agtctgtggt tcgccctgtc
     3121 gcaaggcagc ctgaatatcg tcgcgccgga actgtggcag gcgttgcgcg gcaattctga
     3181 caacgccacg acatatctgg tcgtccacga attgcgcctg ccgcgcgcgc tggcggcttt
     3241 tgccgtcggc ggtctgctcg cgctctccgg cgcgcttctg caagtgctgt tgcgcaaccc
     3301 gctggcggac ccttacgtgc tcggcgtctc gggcggcgcg gcggtagcag cgttgctttc
     3361 catgctcggc ggtctcggca tcggctgggt gcgcggtaac gcctttcttg gcgcgctcat
     3421 ttccatgttc atcgtctttg ggttgagccg ccttggcgcc gtgtggacac agaatcgttt
     3481 gttgctcacc ggtgtggtaa tcgcggcggg ttggggtgcg ctgatcagtc tggtgctggc
     3541 gctggcgccc atggcacaag tacagggcat gctttactgg ctgatcggcg atctgagtta
     3601 tgccacgcgg ccggcggtgg cgctgctggc gctgttgctt gggcttgtcg tctcgctgtg
     3661 gttcgcgcgg gcgctcaatc ttctgatgcg cggcgaaaac accgccgccg cgctgggaga
     3721 aaatcccgcg cggctgcgct tcattattta cctcatcgct tccctgctga ccgccatggc
     3781 ggtcacgctc gccggcagcg tgggcttcgt gggtctggtg attccccata tattaaggtt
     3841 gcttgccggc tcggatcatc gctttctgct gcccgcgtgc gtgctgctcg gcggcggttt
     3901 tctgaccatt gccgataccc tcgcgcgcag tctggcagcg ccggtacaac tgcccgtggg
     3961 agtgatcacc gccttgctgg gcattccggt gtttctctat ctgctgatcc gctcacgctg
     4021 atgctgcgcg cccggaatct ctcgctcacg atcgccggcc gcaccctgtg cgccgatctg
     4081 aatgtaaatc tgcaagccgg cgagaactgg gtgattctgg gcgccaatgg cagcggcaaa
     4141 accacactgc tgcacgcgct ggctggtttg cgcgcgccga acgcgggcgg cgtcagcctc
     4201 gataacaggg aaatcggtgc gatcccgccg cgcgcccgcg cccggcgcct gggcgtgttg
     4261 tttcaggaga cggagtcgga atttcccgcg acagtcatcg aaacggtatt gaccggccgc
     4321 tacccccatc tcgaccgctg gcaatgggaa ggcgcgcagg actatcacat ggccgaggcg
     4381 gcattggcgg cggtcggcct ggatggtctg gcctggcgcc cgctcgccac gctctccggc
     4441 ggggaacggc ggcgggtgga aatcgccgcc ctgctggcgc aggacgcgcc gatctgcctg
     4501 ctggatgagc cgaccaacca tctcgacctg cgccatcagg tacaggttct gaacctgctg
     4561 gccggtcgcg cgcaacagcc gggatatctc aacgttttcg tgctgcacga cgtcaacctg
     4621 gcagcacgct tcggcacaca tggcatcctg ctgttcgaca atggcgaatg ccggcatggc
     4681 ccgctgtccg acctgctgga tcgtccccac ctcgaacggt tgtaccactg ccgtttgcgc
     4741 gaaatccgcg atggcggcag cgtctggttt ttgccggagt gacgccgccc cgggcggtgt
     4801 ggattaatca aggaccgacc gttatacttt ccgccactcg cagcggcact ta
//
LOCUS       DBNGZE010000047         5935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1743167, whole genome shotgun sequence.
ACCESSION   DBNGZE010000047 DBNGZE010000000
VERSION     DBNGZE010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1743167"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="AC16FV_01220"
     CDS             <1..880
                     /locus_tag="AC16FV_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2848715.1"
                     /translation="HADNLRRGALLMLASGLLFSTMGALVKYMSAHLPNEMVVFFRSA
                     MGLLALLPWVLHRGVGHLKTKNLRGHLWRGLAGVSAMYCYFYAIAHLPLAVATLINYS
                     KPLFVTFIDAAWLGEKISRKLWSAIITGFVGIVLILKPGLDLFTPVSLIGVASGLFAA
                     LAMVSIRRLTHTEPTLRIVFYFSAVCTAVSAVPLAWRWQTPDPGLWTLLILMGALASL
                     AQLLLTRAYSHAPAAQVGPFTYSTVVFAATLGWMFWGEIPDLLSFGGTMLVCLAGIMT
                     IRFAGQRLLSEARLAK"
     gene            1023..1553
                     /locus_tag="AC16FV_01225"
     CDS             1023..1553
                     /locus_tag="AC16FV_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848716.1"
                     /translation="MDSLDRAIDLVSRWKADRNKQRAEVLTRLESVIKDCQAGIKVWQ
                     DFLDKPGAPGDQWTIVSWIGPSRAKQLHEINLRAKASVEEVCRMAGPAAGRFVVLDED
                     VIEMAYRQLKPDESGIDAAKAAVVNTQKTMDYLRGLIERARSAKAPAAAGKSPAKKAK
                     KAVAKPAKKKAAPRKK"
     gene            complement(1655..2011)
                     /locus_tag="AC16FV_01230"
     CDS             complement(1655..2011)
                     /locus_tag="AC16FV_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848717.1"
                     /translation="MNGTAIALGDGNDGIDSFGPSDIQHVVFTLSKEPYDMKKSLISL
                     FALAFAMMFGMVHVAGAAPEATADAAAAEAKKEEKKEVKKEKKMKKKAAKKEEKKEEM
                     KGATPATPATPAAPAK"
     gene            2117..3868
                     /locus_tag="AC16FV_01235"
     CDS             2117..3868
                     /locus_tag="AC16FV_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2848718.1"
                     /translation="MSFRFFRLGRLQRKVLLVISVIVIVPMLVAGWLAAEWVSISFEK
                     RLQQWIVDAARSNQNWLQAYQNDAVMLGRVLADDPAYVSKIERDPEEAMPLPMQRISQ
                     ELSINLIQIYTPEKKLMYSSMPVEVNALWERGQTEAVLKVVSKRKTMLAAVGITPIPR
                     QGKPRYYLVLGSLLGQDFTDELAQLTGLKARLYYREGKNYYDLFSTPGKTIALKHLPT
                     PALARLEKDKKPFYSLEAENGQFRGLYTPIVDPTGRVEAIMFSGLERRGVQEVLTNRV
                     TLFLSISLLGVIVGGLAGLLLSRLVVRPLEYLRNGVMQLAGQNFNANVPISSDDELGD
                     LAKAFNAMAARLREARDEQTQRFQKDKLTAMGELSAALAHEIRNPIGIINASAALLDK
                     PDYAPEKKAELTRMIREESLRVANLVQDFLQLSRHRQPSFAVIDPALPLERALELALA
                     GAPAVTVHRDMQHQDAKIQADAGLLQQAWGNIFTNALQAMSGKQAVLRLRSGVEDGQV
                     YLSVEDNGPGVPADIMPRLFEPFFTTKDQGTGLGLTIAFTLTEANGGRLEALPPEGSG
                     ARFVMRFPVYEAMEVAS"
     gene            4099..5490
                     /locus_tag="AC16FV_01240"
     CDS             4099..5490
                     /locus_tag="AC16FV_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2848719.1"
                     /translation="MKRTVLVVDDEANMQKVMRMVLESAGHNVLLAESGETALAQVQN
                     PNLDVVLTDLRMPGMGGEEFVVRCRKERPDVPVIIVTAHGTIRSAVKSIHDGAADYLA
                     KPFEPEQLEIAVHNAIKLRDIMRENARLKAEVSESRGTKRLAGESRVAQQLLEEIRRV
                     APYKTGVLITGESGTGKELVARTIHELSPRRERPWVALNCSAIPRDLMESELFGYVKG
                     AFTGATHNRPGRLEQAQGGTLFLDEIADLDAALQAKLLRVLQEREFSPVGSNQVRAVD
                     VRFIAATNRDLKELVRDGRFREDLLYRLDVYSIVVPPLRSRREDIPLLAQTFLHELAA
                     ETDKKITAFTPAALEALERYVWPGNIRELRNTVERSLLSCEGKTIDIGDLPATVATPS
                     GRAADEKFTLDKMGRKNLDHWLEEVERGVILEALAQTKGVQAHAAKRLGISERSLWHR
                     IKKLNIQINRVIH"
     gene            5496..>5935
                     /locus_tag="AC16FV_01245"
     CDS             5496..>5935
                     /locus_tag="AC16FV_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR2848720.1"
                     /translation="MTTGAYPPAWHQLPAEEIAQHLHTDPRAGLSEDEARARRGQYGA
                     NEIREGRTRGPVAMFLGQFTDFMILVLIAAAIVSGLIGDVVDTLVIVAIVVLNAIIGF
                     VQEYRAERAIAALKQMAALTAHVMRDGQSRMVPASELVPGDVVL"
BASE COUNT         1171 a         1768 c         1842 g         1154 t
ORIGIN      
        1 gcacgcggac aacctccggc gcggcgcgct gctgatgctc gcctcggggc ttttgttttc
       61 caccatgggc gcgctggtca agtacatgtc agcgcatctg ccaaacgaga tggtggtgtt
      121 tttccgcagc gccatgggtt tgctggcgct gctgccgtgg gtgttgcacc gtggcgtcgg
      181 ccacttgaaa acaaaaaacc tgcgcgggca tctgtggcgc gggctcgccg gggtgtcggc
      241 gatgtattgt tatttctacg ccatcgcgca cctgccgctc gccgtggcca cgctgatcaa
      301 ttattccaag ccgctgttcg tgacgttcat tgacgccgcc tggctgggcg agaaaatctc
      361 gcgcaaatta tggtccgcca tcattaccgg cttcgtcggc atcgtgctga tcctgaagcc
      421 gggcctcgat ctgttcacgc cggtttcgct catcggcgtg gcctcgggct tgttcgcggc
      481 gctggcaatg gtgagcatcc ggcgtctcac gcacacggag ccgaccttgc gcatcgtgtt
      541 ttacttcagc gccgtgtgca ccgctgtgtc cgccgtgccg ctcgcctggc gctggcagac
      601 gcccgacccc ggtttgtgga ccctgctcat attaatgggc gcgctggcca gtctggcaca
      661 gctgctgctg acgcgagcct actctcacgc gccggccgcc caggtgggtc ccttcaccta
      721 ttccaccgtg gtattcgccg ccaccctcgg ctggatgttc tggggcgaga ttccggattt
      781 gctctccttt ggcggcacca tgctggtgtg cctggcgggg atcatgacca tccgtttcgc
      841 cggtcagcgg ctgctttccg aagccaggct ggccaaatag ccggacacct ggttttggcg
      901 agtaaaacca gcggattatt tgtttgtgct taccttgtca gtccccctgc cttgtcccaa
      961 aataaataaa caacctgcag gttgtgccac acccttcaag atcaaggaaa acggagataa
     1021 tcatggatag cctggaccga gcgattgatc tggtatcgcg ctggaaagcc gatcgcaaca
     1081 agcagcgtgc cgaagtgctg acgcgccttg aaagcgtcat caaggattgt caggcaggca
     1141 tcaaggtctg gcaggatttc ctcgacaagc ccggtgcgcc gggtgaccag tggaccattg
     1201 tgagctggat tggcccaagc cgcgccaagc agttacacga aatcaatcta cgggcaaagg
     1261 caagcgtgga ggaagtatgc cgcatggccg gtccggcggc gggacgtttc gtggtgctgg
     1321 atgaggatgt tatcgaaatg gcctaccgcc agctcaagcc ggatgaatcc ggcatcgatg
     1381 ccgccaaagc cgcggtggtg aatacccaga agaccatgga ctatctgcgc gggttgatcg
     1441 agcgcgcgcg cagcgccaag gccccggcgg cagcggggaa aagcccggcg aaaaaagcga
     1501 agaaggccgt cgccaaaccc gcgaagaaaa aagccgcgcc caggaaaaaa taattgccga
     1561 aggaaagttg ccggtttgta acaggcaccg atatccataa aaaacaaagc gccccgaaag
     1621 gggcgctttg gtgcagctta aaaactaaac gctattactt ggcgggagcg gccggggtcg
     1681 cgggagtggc cggggtcgcg cccttcatct cttctttctt ctcttccttc ttggccgctt
     1741 tcttcttcat cttcttttct ttcttcactt ctttcttctc ttccttcttg gcttccgccg
     1801 cggcagcgtc ggccgtggct tcaggcgccg cgccggcgac gtgcaccatg ccgaacatca
     1861 tggcgaatgc cagggcaaac aggctgatca gtgacttttt catatcgtaa ggctccttgg
     1921 aaagagtaaa aacaacgtgt tggatatctg atggcccgaa cgaatcaatg ccatcattgc
     1981 catcccctag agcaatcgct gtgccattca tttaatttat aaaaatcaat tagttatata
     2041 tactgcctcc actgcgttaa gcttatattt tgtagcaagc ttcaaaattt gtagttaggg
     2101 gatttcaaga aaaggtatgt cgtttcgttt cttcaggctt ggccgcctcc agcgcaaggt
     2161 gctgctggtg atatccgtca tcgtgatcgt gccgatgctg gtagccggct ggctggccgc
     2221 cgagtgggtt tcgatcagtt tcgagaaacg tttgcaacaa tggatcgtcg atgccgcgcg
     2281 ttccaaccag aactggctgc aggcctacca gaatgacgcg gtcatgctgg ggcgcgtgct
     2341 ggcggacgac ccggcatacg tttccaaaat cgaacgtgat cccgaagaag cgatgccgtt
     2401 gccgatgcag cgcatctccc aggaattgag catcaacctc atacagatat acacgccgga
     2461 aaaaaaactg atgtattcct ccatgccggt ggaggtcaat gccctgtggg aacgcggcca
     2521 gacggaagcc gtgctcaagg tggtgagcaa gcgcaagacc atgctggctg ccgttggtat
     2581 cacgcccatc ccgcgccagg gcaaaccgcg ctactacctc gtgctgggta gcctgctcgg
     2641 acaggatttt accgatgaac tggcgcagct caccggcctg aaggcacgtc tttattatcg
     2701 cgagggcaag aattactacg atcttttttc cacgcccggc aaaaccatcg cgcttaaaca
     2761 tctgcctacg ccagcgctcg cgcggctgga aaaggacaag aaaccctttt acagcctgga
     2821 ggccgagaac ggtcagttcc gcggcctgta tacgcccatc gtggatccca cggggcgggt
     2881 cgaggcgatc atgttcagcg ggctggagcg ccgcggggtg caggaagtgc tcaccaaccg
     2941 cgtcacgctg ttcctgtcga tttcgttgct cggcgtgatc gtcggcggct tggccggcct
     3001 gctgctttcg cgcctggtgg tgcggccgct cgagtatctg cgcaacggcg tcatgcagct
     3061 cgccggccag aatttcaacg ccaacgtgcc cattagttcg gacgacgaat tgggcgatct
     3121 ggccaaggcg ttcaacgcca tggccgcgcg cctgcgcgag gcgcgcgacg agcagacgca
     3181 gcgcttccag aaggacaagc tgaccgccat gggcgagttg tcggcggcac tggcgcacga
     3241 gatccgcaat cccatcggca tcatcaacgc ctcggcggcg ctgctggaca agccggatta
     3301 tgccccggaa aagaaggccg aattgacgcg catgatccgc gaggaaagcc tgcgcgtcgc
     3361 caacttggtg caggatttcc tgcagctgtc gcgccaccgc cagccttcgt tcgcggtcat
     3421 cgacccggcc ctgccgctgg aacgcgccct tgagctagcc ctggctggtg ctcccgcggt
     3481 cacggtgcat agggacatgc agcatcagga tgcgaagatc caggccgacg ccggcctgtt
     3541 gcagcaggct tggggcaata tcttcaccaa tgcgttgcag gccatgagtg gaaaacaagc
     3601 ggtgctcagg ctccggtccg gtgtcgagga cggacaggta tatctgtcgg tcgaggacaa
     3661 tggtcctggc gtgccagcgg atatcatgcc gcgcctgttt gagccgtttt tcacgaccaa
     3721 ggaccagggc accgggctcg ggctcaccat cgccttcacc ctgaccgaag ccaacggcgg
     3781 acgcctcgaa gccctgccgc cagagggcag cggcgcccgg tttgtcatgc ggtttccggt
     3841 atacgaagca atggaggtag cgtcatgagc cgcggagcag taggtgcgac gcgaccgcgc
     3901 tgcgtaggaa taggagccct tgtggagagg agactgctgc gtcggatgcc agcgccgccc
     3961 acgcggtcgc tggcatccgc tccgctgccc gatcccgctt agcgggtccc tcgggcaacg
     4021 ctccgcggcg cccgaattga cgcaggctac cgacaacgtg agtccaagtt atggtgagaa
     4081 attaatggag gtagcgccat gaaacgcact gtgctggtgg ttgacgacga agcaaatatg
     4141 caaaaggtga tgcgcatggt gctggaaagc gccggccaca atgtattgct ggccgagagc
     4201 ggcgagacgg cgctggcgca ggtgcagaac ccgaatctcg atgtcgtgct caccgatctg
     4261 cgcatgcctg gcatgggcgg cgaggaattc gtcgtgcgtt gccgcaagga gcgcccggac
     4321 gtgccggtga tcatcgtcac ggcgcacggc acgatccgtt ccgcggtcaa gagcattcac
     4381 gacggcgcgg cggattatct cgccaagccg ttcgagccgg agcagctcga gatcgcggta
     4441 cacaacgcca tcaagctgcg cgacatcatg cgcgaaaacg cccggctcaa ggccgaggtc
     4501 agtgagtcgc gcggtaccaa acgactggcc ggggaaagcc gggtcgcaca gcaattgctc
     4561 gaagaaatcc ggcgcgtggc gccgtacaag accggcgtgc tgatcaccgg cgaatccggc
     4621 accggcaagg aactggtggc gcgtaccatt cacgagctgt cgccgcggcg cgaacggccg
     4681 tgggtcgcgc tcaactgctc cgccatcccg cgcgatctca tggagtccga gttgttcggt
     4741 tacgtcaagg gtgcgttcac cggcgcgacg cacaatcgcc cgggccggct ggagcaggca
     4801 cagggcggta cgctgttcct ggacgagatc gccgatctcg atgccgcgtt gcaagccaag
     4861 ttgctgcgcg tgttgcagga gcgcgagttt tccccggtcg gctcgaatca ggtgcgggcc
     4921 gtggatgtgc gtttcatcgc cgccaccaac cgcgatttaa aggagctggt gcgcgacggg
     4981 cggttccgcg aggatcttct gtatcgtctg gacgtataca gcattgtggt accgccgctg
     5041 cgcagccggc gcgaggatat tccgctgctg gcccagacct tcctgcacga actcgccgcc
     5101 gagaccgaca agaagatcac ggccttcaca ccggcggcgc tggaagcact ggaacgctat
     5161 gtttggcccg gtaacatccg cgaactgcgc aacaccgtgg aacgctcgct gctgtcatgc
     5221 gaggggaaaa ccatcgacat cggggatctg ccggctaccg tcgcgacgcc gtccggaagg
     5281 gccgccgatg agaaattcac gctcgacaaa atgggcagga aaaatctgga ccactggctg
     5341 gaagaagtcg aacgcggcgt gattctcgaa gcgctggcgc aaaccaaggg tgtacaggcg
     5401 cacgccgcca agaggctggg tatctctgag cgcagcctgt ggcaccgcat caagaaactt
     5461 aacatccaga tcaaccgcgt tatccactga acccaatgac gaccggtgcc tatccgcctg
     5521 cttggcatca gctgccggcc gaggaaatcg cccaacatct ccacaccgac ccgcgcgctg
     5581 gtctgagcga ggacgaggcg cgggcgcgtc gcggacaata cggcgcgaac gagattcgcg
     5641 agggccggac caggggaccg gtcgcgatgt tcctcgggca gttcaccgat ttcatgatcc
     5701 tggtgttgat cgccgccgcg atcgtgtcgg gcctgatcgg cgacgtggtg gacacgctgg
     5761 tgatcgtggc catcgtggtg ctaaacgcca tcattggctt tgtgcaggaa taccgcgccg
     5821 agcgcgccat tgccgcgctc aagcaaatgg cggcgctcac cgcgcacgtt atgcgtgacg
     5881 ggcaatcgcg gatggtgcca gcgagcgagc tcgtgcccgg tgacgtggtg ctgat
//
LOCUS       DBNGZE010000048         2677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_215746, whole genome shotgun sequence.
ACCESSION   DBNGZE010000048 DBNGZE010000000
VERSION     DBNGZE010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_215746"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /gene="nuoG"
                     /locus_tag="AC16FV_01250"
     CDS             <1..1972
                     /gene="nuoG"
                     /locus_tag="AC16FV_01250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR2848721.1"
                     /translation="LDGMVVHTRSEKARTAQKGTMEFLLINHPLDCPICDQGGECELQ
                     DLAVGYGKGDSRYTEMKRIVKDKDIGPLIATEMTRCIHCTRCVRFGEEIAGVMEFGGL
                     GRGEHMEIRTFLDRSVDSELSGNVIDLCPVGALTSKPFRYTARTWELEDHASISPHDC
                     VGANITVQTRRGHVMRVLPRTNEAVNECWLSDRDRFSYEALNSEDRLRVPMIRRGTQW
                     EETDWPTALEFTVAGLRKTLEAHGPESLGALASPISTSEEFYLLQKLVRALGSGNVDH
                     RLRQMDFSDDAMAPPFPALGRPIAELESLDCALLVGANPRKDQPLINLRLRKSALKGA
                     KIFAINPLDYDFNYKLAGGIVCTPADMLRALAEVAGAMAGLKNISLPASLKTRFGNIT
                     PKAAEQAMAQALHQGKRVSILLGTFALGHPQAATLRALAQFIAELSGAKFGQLPEANA
                     AGAWLAGCVPHRGVAGKPATKGRHALDMLRNPLKAWMLFGVEPEFDCLDGAAAAAIRT
                     ADFVVMMTTFKPSPYRTRAVEYADVWLPLAPFTETDGTFVNAECRSQTFAAATTPLGE
                     SRPGWKILRMLGHVMGLSGFEQAGVEEIRHEMNQPVAATAVSGFTLPPEDPAMSPATG
                     QLMRLAEVPMYAVDPIVRRAPALQKNADNPPP"
     gene            2039..2176
                     /locus_tag="AC16FV_01255"
     CDS             2039..2176
                     /locus_tag="AC16FV_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848722.1"
                     /translation="MSQGVARLDLVLDERVPDGCVLVPAGCPETSMLGAHGPATVKGA
                     S"
     gene            2185..>2677
                     /locus_tag="AC16FV_01260"
     CDS             2185..>2677
                     /locus_tag="AC16FV_01260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR2848723.1"
                     /translation="MIEFWNWFPDWSQIALINLGKIVLILVPLLLSVAYLTYAERKII
                     GYMQIRIGPNRVGPKGWLQPIADAVKLLLKEIIVPTKSSSFLFIFAPILAFVPALAAW
                     AVIPFTGTLWLTNIDAGLLYILALTSMGVYGVIIAGWASNSKYAFLGAMRSAAQIVAY
                     EIAM"
BASE COUNT          495 a          800 c          856 g          526 t
ORIGIN      
        1 gttggatggc atggtggtgc acacccgctc cgaaaaggcg cggacagcac agaaaggcac
       61 aatggaattt ctgctgatca atcatccgct cgattgcccg atctgtgatc agggcggcga
      121 gtgcgagttg caggatctgg cggtgggtta cggcaaaggc gactcacgct acaccgaaat
      181 gaaacgcatc gtgaaggaca aggacatcgg accactgatt gccaccgaaa tgacgcgctg
      241 catccactgc acccgctgcg tgcgtttcgg tgaagagatt gccggcgtca tggagttcgg
      301 cggcctgggg cgcggcgagc acatggagat tcgcaccttc ctcgaccgca gcgtggattc
      361 cgaactctcc ggcaatgtca tcgatctctg cccggtcggc gcgctgacgt ccaagccgtt
      421 ccgctacacc gcgcgcacct gggagcttga ggaccatgcc tcgatcagtc cgcatgattg
      481 tgtcggcgcg aatatcaccg tgcagacacg ccgtggacac gtgatgcgtg tgctgccacg
      541 caccaacgag gcggtcaatg aatgctggct gtcggatcgc gaccgcttca gttacgaggc
      601 cctgaacagc gaagatcgcc tgcgggttcc gatgatccgg cgtggtaccc agtgggaaga
      661 aaccgactgg cctacggccc ttgaattcac cgtggcgggg ctcaggaaga cgctggaagc
      721 gcatgggcca gagagtctgg gtgcgctggc gtcgcccatt tccacatcgg aagaattcta
      781 tttactgcag aaactggtgc gcgcgctggg cagtggtaac gtggatcacc gcctgcgcca
      841 gatggatttc agcgatgacg ccatggcgcc gccgttccct gcgctcgggc ggccgatcgc
      901 ggaactggaa agtctcgatt gcgcgctcct ggtcggtgcc aatccgcgca aggaccagcc
      961 gctgatcaac ctgcggctgc gcaagagcgc gctcaagggc gcgaaaattt tcgccatcaa
     1021 tccgctcgat tacgacttca actataaact cgcgggcggg attgtctgca cgcccgccga
     1081 catgctgcgc gcgctcgccg aggtcgccgg cgccatggct ggattgaaga atatttcctt
     1141 gccggcgtca ttgaagacac gttttggcaa tatcacgccc aaagcggcgg aacaggccat
     1201 ggcccaggcc ctgcatcagg gcaagcgtgt ctcgatcctg ctcggaacgt ttgcgctcgg
     1261 ccatccccag gcggcgacgt tgcgcgcgct ggcgcagttc attgccgaat tgtccggcgc
     1321 gaaattcggt cagctgccag aggccaatgc cgccggcgcc tggctcgccg gctgtgtgcc
     1381 gcatcgtggc gtggcaggca aaccggcgac gaagggtcgt catgcgttgg atatgctgcg
     1441 caatccgctc aaggcctgga tgttgtttgg cgtcgaaccg gagttcgatt gtctggatgg
     1501 tgcggctgcc gcggctatac gcacggcgga ctttgtcgtg atgatgacga cgttcaagcc
     1561 ctcgccatat cgcacgcgcg cggtggaata tgcggatgta tggctgccgc ttgctccctt
     1621 caccgaaacc gacggcactt tcgtcaatgc cgaatgccgg tcgcagacat tcgcggcggc
     1681 gaccacgccg ctcggcgagt cacggccggg ctggaaaatc ctgcgcatgc tgggacatgt
     1741 gatgggcctt tccggattcg agcaagcggg agtcgaggaa atacggcatg agatgaatca
     1801 gccagttgct gcaactgcgg tttctggttt tactttgccg ccggaagatc cggcgatgtc
     1861 acccgcaacc ggccagctta tgcgtctggc ggaagtgccc atgtacgcag tcgatcccat
     1921 tgtgagacgc gcgcccgcgc tgcaaaaaaa cgccgacaat ccaccccctt aggcgcgcga
     1981 taacgcagct cagtccggca agctcggtta caagggtggt gagaacgtgc aggttgcgat
     2041 gtcgcaggga gtggcgcgcc tcgatctcgt gctggatgaa cgtgtgccgg acggttgcgt
     2101 gctggtgccc gccggctgtc cggagacgtc catgctcggc gcgcacggcc cggcgaccgt
     2161 gaagggagcg tcatgagcac gctgctgatc gagttctgga actggttccc cgactggtcg
     2221 cagatagcgc tgatcaatct cggcaagatc gttcttattc tcgtgccgct gctgttgtcc
     2281 gtggcatacc tgacttatgc cgaacgcaag atcatcggtt acatgcagat acgcatcggc
     2341 ccgaaccgcg tcggccccaa gggctggctc cagcccatcg ccgatgcggt gaagctgctg
     2401 ctcaaggaaa tcatcgtccc caccaagtcg agcagctttc tgttcatctt cgcgccgatc
     2461 ctggcgtttg tgccggcgct ggcggcgtgg gcggtgattc cgttcaccgg cacgctgtgg
     2521 ctcacgaaca ttgacgccgg tctgctgtat atcctcgcgc tcacgtccat gggggtatac
     2581 ggggtcatca tcgccggctg ggcgtcgaat tcgaagtatg cgttcctcgg ggcgatgcgc
     2641 tcggcggcgc agatcgtcgc ctacgagatc gcgatgg
//
LOCUS       DBNGZE010000049         1882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_215751, whole genome shotgun sequence.
ACCESSION   DBNGZE010000049 DBNGZE010000000
VERSION     DBNGZE010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_215751"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1512
                     /locus_tag="AC16FV_01265"
     CDS             157..1512
                     /locus_tag="AC16FV_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR2848724.1"
                     /translation="MRQQHVQPSVQAKPNLQAQPQLKLDTRQQQRAERALRQSEDREL
                     RRVPRSQRAQRREQIQNARQQRALSRQQNVPPNVLQAQPNARTLRANRRDGTARVTQD
                     AARHGRFAWRFAQRAGGVNARDRFAHVAAHRAWRQGHRASFVARYGPLFWPYAYSDIF
                     DYTFWPYGYDDGYWAYAYDDFFDGVFWGEAGPPQDYAYAAPSTAPLAPSPSYVGVQEL
                     CRQPGSGITAWPFAEIERKVGLNADQKQLLGDVRKASQDAAAVFKASCPPENAFPLTP
                     PGRLDSMTARLDATLQTVQTVRPALERFYDSLSDEQKERFNELGPKNTAANAEASEAS
                     QAAETCKQPKQGLANLPIEKIEDALNPTDAQEAELNKLQDATSKAVSIMQAACPDETP
                     ITPPGRLDAMEKRLQAMIDAAKTVKPALEGFYSSLTSEQKARFNRIGRSLAETTGQSG
                     N"
     gene            1702..>1882
                     /locus_tag="AC16FV_01270"
     CDS             1702..>1882
                     /locus_tag="AC16FV_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2848725.1"
                     /translation="MTKTAHGTAACIAAIIFALAAATAEAKDKVKVGFIGPLTGGVSV
                     NGLGGRNSADLAVRLR"
BASE COUNT          423 a          589 c          555 g          315 t
ORIGIN      
        1 caggtgccaa cggcacgcat cgcgccgcgg caagggccgc aacacgcggt caccccgcga
       61 gttcgggctc cacaacgcac cgtcgcgccg gtccaacggc aggctccgac cgtcaatcgc
      121 actgtacagc agcatcaact gcgccaggag cgtgcattgc gacagcagca tgtgcaaccg
      181 agcgtgcaag caaagccgaa cctacaggca cagccacaac ttaaactcga tacacgtcaa
      241 cagcagcgtg ccgagcgcgc cctgcggcag agcgaggatc gtgaactgcg ccgcgttccc
      301 agatcgcagc gggcacagag gcgtgagcaa attcaaaatg cgcgtcaaca gcgtgcgctt
      361 agccggcaac aaaacgtgcc gcccaatgtg ttgcaggcgc agccgaatgc gcggacctta
      421 cgcgcgaacc ggcgcgacgg taccgcacgt gtgacgcagg acgccgcacg gcatggccgt
      481 ttcgcctggc gctttgcaca gcgggccggt ggcgtcaacg cgcgcgaccg tttcgcgcat
      541 gtggcggcgc atcgtgcctg gcgacaaggc catcgtgcaa gcttcgtcgc ccggtatggc
      601 ccgttgttct ggccctacgc ctattccgat atttttgact acacattctg gccgtacggc
      661 tatgacgacg gctactgggc ttacgcctat gacgacttct tcgacggtgt attctggggc
      721 gaggccggcc cgccgcagga ttacgcttat gccgcaccat ctaccgcgcc gttagcgccg
      781 agcccgagct atgtcggcgt gcaggaactg tgcagacagc ccggcagcgg aattacggca
      841 tggccgtttg ccgagatcga acgcaaggtc ggtctgaatg ccgatcagaa gcaattgctt
      901 ggcgatgtgc gcaaggcgag ccaggatgcc gccgcagtgt tcaaagcgtc atgtccgccg
      961 gaaaatgcgt tcccactcac gcctccgggc cgcttggact caatgactgc tcgccttgat
     1021 gcgaccctac aaacggtgca aacggtgcgc ccggcgctgg agaggttcta cgattcgctc
     1081 agcgacgagc aaaaggaacg cttcaacgag ctcggcccaa agaacaccgc cgccaatgcc
     1141 gaggcgagcg aagcctcgca agcagccgaa acctgcaagc agccaaaaca aggccttgcc
     1201 aacctgccaa tcgagaaaat cgaagacgcg cttaacccca ccgatgcgca agaggccgag
     1261 ctgaacaagc tgcaggatgc gaccagcaaa gcggtgtcga tcatgcaagc cgcttgtccg
     1321 gatgagacgc cgatcacgcc gccagggcgg ctggatgcga tggaaaagcg cctgcaagca
     1381 atgatcgacg ccgccaagac agtgaagccg gcgctagagg gcttttacag ctcgctcact
     1441 agcgagcaaa aggcgcgctt taatcgcatc ggccgctcgc tggcggagac gaccggacag
     1501 tcgggcaact gattgcggcc actgtgatga aatgaaaaag ggtgcccctg tagccgccct
     1561 tttctatccg catctgacca cctacgacat cctgctctgt tttttacaac aaaaatacgg
     1621 gcttgccgta agtcctctcc cgcgcaacta tgcccggaac tgccaaaacc attttgggat
     1681 aaaacgacgg agggaggacg tatgaccaaa acagcgcacg gcacggcggc ttgtattgct
     1741 gcaattatat ttgctctcgc agcagcgacc gcggaagcca aagacaaagt taaggtcggc
     1801 ttcataggtc cactcaccgg cggtgtttct gtgaacggtc ttggtggtcg aaattcggcc
     1861 gatctcgccg ttcgcctgcg ca
//
LOCUS       DBNGZE010000050         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_701909, whole genome shotgun sequence.
ACCESSION   DBNGZE010000050 DBNGZE010000000
VERSION     DBNGZE010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_701909"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..462)
                     /locus_tag="AC16FV_01275"
                     /pseudo
     CDS             complement(112..462)
                     /locus_tag="AC16FV_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(426..1751)
                     /locus_tag="AC16FV_01280"
     CDS             complement(426..1751)
                     /locus_tag="AC16FV_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2848726.1"
                     /translation="MRRLMQEILEDEHYKVAAVENAAAAREAVKKQRPDLVLLDIWMP
                     GTDGISLLREWSQAGPLEMPVIMISGHGTVETAVEATRLGAYDFIEKPVSMGKLLVTI
                     ERALENDKLRRENLRLRRQAEPAATLTGKSALMQQLRENIERIAATDAWVLITGEAGS
                     GKAVAARYLHARSARKDSPFVEVSLTAITPSHAAAQLFGQENKGAVTPGSFEQAAGGT
                     LLLNEIGGLDLAMQARLLDALEQKRFTRVGGHEAIPMDARVIAASDQDLRKAVEEKRF
                     REDLYYRLNVVPLRVPSLREHHEDVPELINFYVNWMADNEHLPYRKFTTGALNLLRNY
                     SWPGNVRELRNVVQRLLILHRGEEVTETEVEQTLGSPAPVSPESYPPTLMEMPLRDAR
                     DQFEKAYLEHHIKRTGGNVAEVARLSGMERTHLYRKLKQLGVNPKPPRE"
BASE COUNT          259 a          546 c          561 g          386 t
ORIGIN      
        1 cgcgctccgg gggagcgctt ctttgcgtcc tttcttgtcg cgacaagaaa ggacgtcgcc
       61 ttcgggtgcg agaacccgat ttaaatacgc cgccggaggc ggctcgacaa attatttctg
      121 caaacgctta agaaaaaccg tcatctcttt cgccgcctgt ttgtcgcctt tttcttccgc
      181 gactttaatt ccatcttcgt aggctttgat cgcctcttct ttatgtcccg cgtcggtcag
      241 tgccttgccg agcagtttcc acgcggcgga atatttaggg tctttcgcca cggcttggcg
      301 cagatgttcc gcggccttgc cgggttggtt gagtttcaga tactcgacgc cgaggctgta
      361 gcgcagcagc gccgagtcct ggccgcgcgc gagcatggct tccaggttgt cgacgatcgc
      421 cattgttatt ccctgggcgg cttgggattg acgccgagct gcttgagctt gcggtagaga
      481 tgcgtgcgtt ccatgccgga gaggcgcgcg acctcggcga cgttgccgcc ggtgcgtttg
      541 atatggtgtt caagataggc cttctcgaac tggtcgcgcg cgtcgcgcag cggcatttcc
      601 atcagtgtcg gcgggtagga ttcgggcgag accggcgccg gactgccgag ggtctgttcg
      661 acctcggtct cggtcacttc ttccccgcga tgcaggatca ataatcgttg caccacgttg
      721 cgcagctcgc gcacgttgcc cggccaggaa taattgcgca gcaggttgag cgcgccggtg
      781 gtgaacttgc ggtacggcag gtgttcgttg tccgccatcc agttgacgta gaaattgatc
      841 agctccggca cgtcctcgtg gtgttcacgc aggctcggca cgcgcagcgg cacgacgttg
      901 agacggtaat aaaggtcctc gcgaaaacgc ttttcctcca ccgccttgcg caggtcctga
      961 tcgctggcgg cgatgacgcg cgcgtccatg gggatggctt catgaccgcc gacgcgggtg
     1021 aagcgtttct gttcgagtgc atcgagcagg cgcgcttgca tggccaagtc gaggccgccg
     1081 atttcgttga gcagcagcgt gccgccggcg gcctgctcga agctgccggg cgtgaccgcg
     1141 cccttgttct cctgtccgaa caactgtgcc gctgcgtgcg atggtgtgat cgcggtcagg
     1201 ctcacctcca cgaaagggct gtccttgcgc gcgctgcggg cgtgcaggta acgcgccgcc
     1261 acagccttgc cgctgccggc ctcgccggtg attagcaccc aggcgtcggt ggcggcgatg
     1321 cgctcgatgt tctcgcgcag ctgctgcatc agcgcgctct tgccggtgag cgtagcggcg
     1381 ggctcagctt ggcgccgcag gcgcaggttt tcgcggcgca gcttgtcgtt ctccagagcg
     1441 cgctcgatgg tcaccagcaa cttgcccatg gataccggct tttcgatgaa gtcataggcg
     1501 ccgaggcgcg tggcctcgac cgcggtctcg accgtgccat ggcccgagat catgatcacc
     1561 ggcatctcga gcgggccggc ctgcgaccac tcgcgcagca gactgatgcc gtcggtgccc
     1621 ggcatccaga tgtcgagcag caccaggtcc ggccgttgtt ttttcaccgc ctcgcgcgcc
     1681 gccgcggcgt tctcgaccgc cgccaccttg taatgttcgt cctcgaggat ttcctgcatc
     1741 aaccggcgga tg
//
LOCUS       DBNGZE010000051        11966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1188085, whole genome shotgun sequence.
ACCESSION   DBNGZE010000051 DBNGZE010000000
VERSION     DBNGZE010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1188085"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1724)
                     /locus_tag="AC16FV_01285"
     CDS             complement(273..1724)
                     /locus_tag="AC16FV_01285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HIR2848727.1"
                     /translation="MLRTIARAVLAASLLYPTGQLPAYAGNTNLPDLGDESIAVISPA
                     QERKLGEDLMRQSRRTLAFVNDPEINEYIQSLGQRLVSHSDNPQQDFRFFVIDNSTIN
                     AFAVPGGFIGVHTGLILATQNEAELASVLAHETAHITQRHIPRMIAESQRTSLPALAA
                     VLAGILLASSGHQGGEAAIALTSAAVAQKGINYTRSFEEEADRIGMQILAQSGFDPRA
                     MPAFFEQMQNLNRLNDTSLPEFLRTHPVTTNRIADSRNRAEQYRARQSPDSSDFQHVR
                     AKIRALVPGNTEEIVRGFRENLAQRKYPDANAERYGYVVALLRARQLATARTEIQKLL
                     EQQPNRTSYRILQAETEMTAGNYKQALAIYTDAYAKTPTSAPLMQYYVSALLKTEKYR
                     QARDLLKTALQRRPEDPELYRMMAQAAGGNGAKYEAHKAMAEHYYLSGNPHAAIEQLQ
                     LAGRFAGDNFYLQSGLEARIAAIKEEIALYQKK"
     gene            2162..2386
                     /locus_tag="AC16FV_01290"
     CDS             2162..2386
                     /locus_tag="AC16FV_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR2848728.1"
                     /translation="MADQDLDARGLNCPLPILRAKKALNTLTSGQTLRIVATDPGSVK
                     DFQAFAKQTGNQLMESNEANGEYTFILKKA"
     gene            2671..3108
                     /locus_tag="AC16FV_01295"
     CDS             2671..3108
                     /locus_tag="AC16FV_01295"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR2848729.1"
                     /translation="MGAVRGCNLPDDLMYNVESNVWVKREADGNVTVGLTAYACSLAG
                     EIVSYTPKKVGKAVEKDKSCATVESGKWVGPVKTPVAGEVIAVNDAVAAKPGTINQDP
                     YAQGWVVKVKPANWDADTASLKTGAAAMAAFEAKMQADGFKGC"
     gene            3157..4305
                     /locus_tag="AC16FV_01300"
     CDS             3157..4305
                     /locus_tag="AC16FV_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR2848730.1"
                     /translation="MAKWRQITESIETLEGLALRDDLVAELEQRRARSPSAKSGIHFY
                     TPTFKSYQTSEINGCGRNAWPAVSITGSDCKLQCDHCKAKILEPLIAARTPEQLWTTV
                     EDLVAGGAQGMLLTGGSNHRNEVEYDPYYPVVRRIKDAWPSFKIAVHTALVDAAQAQR
                     MEDAGIDVAMMDVIGAQETVTQVYHLKRPVEDFERTLETLVATKMKVVPHIVIGLHYG
                     RLLGEWKALDMIRRHKPDALVLVVVMPMYATPKRPFVTPDPHEVGRFFLEVREALPDI
                     PLLLGCARPPGAAKTLIDTYAVMAGLNGMAHPSDGMVELAARLERPVRVTPACCSIAV
                     GDEVMALESEDQSLTLDIQTILQQEQQRRRLKGIPVISKAAAGGCRGT"
     gene            4302..5414
                     /locus_tag="AC16FV_01305"
     CDS             4302..5414
                     /locus_tag="AC16FV_01305"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR2848731.1"
                     /translation="MIPSSREPWRLIDTGLRPAAENIAINRAMLEAHQEGLIPHTLRF
                     LRFQPSALVGFHQSVEQELRADYCAAHGITIQRRITGGGAIYFDETQIGWELYLHRRV
                     LGTADMQEISRRICEMAAVGIRCLGVDAQFRPRNDIEVNGRKISGTGGAFEGDSIMYQ
                     GTLLIDFDIEKMLRVLRIPAEKLSDKAIASARERVANLKDLLGSLPDMETVKQSLTQS
                     FAEGFNVEFEPVAALSAPEQEKFHQALAEIGHPDWINLVSRPLTDAPMLEGLHKADGG
                     LLRVALTVDRPANRIKQAWFTGDFFVNPRRMVLDLEAALRDSPLADYRHTVNEFFRHY
                     PVEMLRLRPEDFSHTIENALAAFVADSVPVAGGVTP"
     gene            5411..6475
                     /locus_tag="AC16FV_01310"
     CDS             5411..6475
                     /locus_tag="AC16FV_01310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2848732.1"
                     /translation="MTRTVDVLVVGAGPGGSAAAWRAAKAGCRVLVVEKKKRIGEPVQ
                     CAEFIPGPMVDYVRDDGVLAQHIEGMKTYLPSGTVSPSPFPGLMVDRAAFDRALAARA
                     ESAGAEIMANTVLVALDASQRVAHLRHDDRTSDIHYQILVAADGPHSTAARLLGLMPL
                     PVIQTRQYTVPLKAAYADTDIWLSDEFPGGYGWLFPKGALANLGLGIDRCFEPDLKTP
                     LDHLHEELIRQGRVGSEIVQRTGGAIPVGGLRERLSYKNTLYVGDAGGFTHPITGAGI
                     AAAVQSGERAGLAAMEYMNGGVRALENYDEDMHEQYGPTLTRALARRAELAHYWRGAR
                     ANDDPVQRRGWIAFNEYFAS"
     gene            6492..7628
                     /locus_tag="AC16FV_01315"
     CDS             6492..7628
                     /locus_tag="AC16FV_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019612068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR2848733.1"
                     /translation="MSAAADTDSIPVTFYRPNYHIKTPAMRSPEYVQLSTAAAITLGL
                     IDGHMHRTACTHCLNLLVTYPEGCRANCAYCGLARHREETRDYADRNFIRVDWPAVQY
                     DEVIARVKKGEDKDQFERMCISMISHPDSNDDTFILLEKWVREVPQIPVSILSNPTTL
                     DYDDLVRMRDLGADIFTVALDAVTPEIFDRTRGKGVDSPHKWEKYWRTLEWAAEIYGP
                     EKFGAHLICGMGETEQEILDVAQKIRDMGGHNHMFAFFPERGSLMEDWEPVPRDQWRR
                     VQLARYLIDYADGHVSRMRFDASGRLVDYGFDKDALEAVMQSGKPFQTSGCPGRQNDI
                     SACNRPYGDSMPADIRSFPFALDARDIERVRRQMLGENICTYEE"
     gene            7972..9309
                     /locus_tag="AC16FV_01320"
     CDS             7972..9309
                     /locus_tag="AC16FV_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2848734.1"
                     /translation="MSGMKRNLVLSLAVAAAVAAPTAFATNGYFQHGYGMKSSGMGGV
                     STAMTRDAFGGANNPATMVWVGNRMDIGVTWFNPERSASRSGALDTTMNGSADSDSTD
                     FLIPEFGYNHMLNPNMSFGVTVYGNGGMNTDYPGGTLPLTGGTCQGFNPAGAGGAPYN
                     LLCGSGSLGVDLMQLIIAPTWSYKFNQNSSVGVSPLLGYQRFKAEGIQSFSGFSTDAT
                     KLTNNGYDSATGVGLRLGWYGRMSDAVSLGAAYSTKIAMSKFDKYAGLFAEQGDFDIP
                     SNYNLGATFKVGPTVLLGIDYQKIKYTDVKSVSNPSDYGLGGAGIANSLGGSGGRGFG
                     WGDIDVWKIGVEYQHSKDLVLRAGVDKSDNPIKSSDVTFNILAPGVVENHYSVGFTLA
                     MDKKSELTGAFTYVAENKVSGSSLFNNFTGGTSGTEEIKMHQNVLGIAWSNKW"
     gene            9443..10315
                     /locus_tag="AC16FV_01325"
     CDS             9443..10315
                     /locus_tag="AC16FV_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848735.1"
                     /translation="MTSQAAAEALKPFILGDALPGDVKQVSELVKTKLTAQGFEVVGS
                     YSPFPGAMVICVTNNDLKTAAAKAFNGGFGAAQRVAVTDVKGKIQVSYVNPAYIGTAY
                     GLGKLEGVSAKMKTALGAVKPFGSANGIEEERLVPGQYRYAMFMPYFKDVDILKEHPD
                     YKTAVETVEKNLAAGLGGTKKVYRIDIPGKQVSVFGVAIPKGDGPDKGNKDTDKEIID
                     IVDYGDLKHTAYLPYELMVDGNRVIAMRARYRIALNFPDTKMAGEHGFTKIMTAPWGI
                     QVALEAVAGFKREL"
     gene            10502..11662
                     /locus_tag="AC16FV_01330"
     CDS             10502..11662
                     /locus_tag="AC16FV_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR2848736.1"
                     /translation="MSESANTNIGANPAESAGATATISSTTDPLKLLSQVLAHAVRVN
                     ASDIHLRTRNHPILRVDGNLRALREIPPLPPEFMERLSRTMMSARLLAIFEKSHQVDL
                     SIGFKDIGRVRANIFFQRGSIGMVLRVISTNIPTPEELFLPDIVMALTKLERGLVLLT
                     GATGSGKSTTLASLINSINLSRSEHIITIEDPVEFLFSEKRSIITQREVGIDTNTFAE
                     AMRAALREDPDVILLGEMRDPETIETAMTAAETGHLVFSTVHSPAAAETISRIVTSFP
                     PEAQVGVRAKLAQNLRGVVSQRLLPRKNNQGRIVACEVLTVSALAREYILDPLKIKEL
                     GDLIRKGTMAEGMLSFDTSLLNLVKQGHIDDETALFYATSPTDLKLKLEGFA"
BASE COUNT         2510 a         3337 c         3563 g         2556 t
ORIGIN      
        1 accatgactc cacgatcagg acaggggttc tcaggtatag ggaatcaaac aagccttcaa
       61 ccacccttaa cgtgccggga aaaacaggat acctttctca ccgggaatga caagtcctct
      121 tataaaaata tacggcagtt tacattatcg taaataagca cctgttaatg tagcctggct
      181 tcgatggatt cggagtcggg cattcaatgt ggccattatt gggttctaga cgcacagtca
      241 ccggaaaata ttcctcactt tcaccgtgtg cgtcattttt tctggtaaag ggctatttct
      301 tccttaatgg cggcgattct ggcctcgagg ccggattgca gataaaaatt gtctcccgcg
      361 aaacgacccg ccagctgaag ctgttcgatt gcggcatggg gattgccgct cagataatag
      421 tgttcggcca tggccttgtg ggcctcgtat ttggcgccgt tgccgcccgc ggcctgcgcc
      481 atcatgcggt agagttcagg atcctccggc cgccgttgca gcgcggtttt gagcaggtcc
      541 ctggcctgac ggtatttttc ggttttcagc agcgcactga cgtaatactg catcagcggc
      601 gcactcgtgg gcgtcttggc gtaggcgtcg gtgtatatgg caagtgcctg tttgtaattc
      661 cccgccgtca tctcggtctc cgcctggagt atgcggtagg aagtgcggtt tggctgctgt
      721 tcaagaagtt tctgaatttc tgtgcgtgcc gtcgcgagct gccgcgcacg cagcaacgcc
      781 acgacatagc catagcgttc ggcattggcg tcgggatatt ttctctgcgc cagattttcc
      841 ctgaatccgc gcacgatctc ttccgtattg ccggggacga gagcgcggat tttcgcgcgc
      901 acatgctgga agtcgctgct gtcagggctc tggcgtgcgc ggtattgttc ggcgcggttg
      961 cgtgagtcgg cgatgcggtt ggtggtgacc ggatgggtgc gcagaaattc cggaaggctg
     1021 gtgtcgttca accggttgag attctgcatc tgctcgaaaa acgccggcat cgcacgcggg
     1081 tcgaagccgg attgcgcgag gatctgcatg ccgatgcggt ctgcttcctc ttcgaagctg
     1141 cgcgtgtaat tgattccttt ctgggcgacc gcggcgctgg tgagcgcgat cgcggcctcg
     1201 ccgccctgat gaccggaact ggccagcagg atgcccgcca gcaccgcggc cagagccggc
     1261 aggctggtgc gttgcgattc cgcgatcatt cgcgggatgt gtcgctgcgt gatatgtgcg
     1321 gtttcatgcg ccagcaccga agcaagctcg gcctcatttt gagtggccag aatcaagccg
     1381 gtgtgcacgc cgatgaatcc gccgggcacg gcgaaagcgt tgatggtcga attgtcgatg
     1441 acgaagaacc ggaagtcctg ttgcgggttg tcgctgtgcg acaccagccg ctgacccagc
     1501 gactggatgt attcgttaat ttccggatcg ttcacgaacg cgagggtgcg gcgcgactgg
     1561 cgcatcaggt cttcgcccag cttgcgttcc tgcgcgggcg atatcaccgc gatggattca
     1621 tcgcccaggt cgggaagatt ggtgttgccg gcataggcgg ggagctgacc ggtcggatac
     1681 aacagactgg ccgccagtac cgcccgcgct attgtgcgaa gcatgttttt cattgtatgg
     1741 ctgttatgca aaaacatcga tccggagaca gcccgtgaaa cgtcgatatt cctttaccga
     1801 ttgccgctct caggaacatg cggccacgga tactgcccgc agtttgaagc gatttaattt
     1861 ccaacagcga ccgctgtgat gggttcaggt ttacctctcc agggtcgtgt tgcgtgcaga
     1921 ctccgcgatt ccagtctgga tggcatctaa atacctatat ttcaataggt taccctggtt
     1981 actgagagcg cagaaatcgc gatttgttcc ccgtgccgtg ccacggaagc ggcgattctc
     2041 agggagaatg agtgggtcag gagttttgag cggccagttt gatactaaat tagaaatcta
     2101 taatatatcg ccctctgact attatcattt ctagaaattt cgtttttttt gaggaggaaa
     2161 aatggctgat caagatctcg atgcacgcgg gctgaattgt ccgcttccca ttctgcgcgc
     2221 caagaaggcg ctgaacacgc tgaccagcgg ccagacgctg cgcatcgtgg cgaccgatcc
     2281 gggttcggtg aaggattttc aggcgtttgc caagcagacg ggcaatcagt tgatggaatc
     2341 caacgaagcc aacggcgaat acaccttcat tctgaaaaag gcataaataa aataatccgt
     2401 tacaggaaaa gacaggtcgg attctggaca aggggcgttg agccccggga gagggagtaa
     2461 gtacgcgcgg cgatgacgga caaagcgcca atctcccaag atgagttgga acaataaagg
     2521 ggcgcctatg gcgccccttt attgtttatg cggctgcatc atgggaagaa tttgaggcac
     2581 gtcaatactt tgaggcaggg acgtgcctat tcttctccgt ggcatcaacg agcaggcaac
     2641 atccgttcaa cacacagcag gaggccggaa atgggcgcag tgagaggatg caatcttccc
     2701 gatgacctga tgtacaacgt ggaaagcaat gtctgggtaa agcgcgaagc ggatggcaat
     2761 gtcactgtcg gcttgaccgc ctatgcctgc tcgctcgcgg gtgaaatcgt ttcctacaca
     2821 ccgaagaagg tcggcaaggc cgtcgagaag gacaaatcct gcgccacggt ggaatccggc
     2881 aaatgggtcg gtccggtgaa gacgccggtg gcgggtgagg tgatcgcggt gaatgacgcg
     2941 gtggccgcca agcccggtac catcaaccag gatccttatg ctcagggctg ggtggtgaag
     3001 gtgaagcccg ccaactggga tgcggatacc gccagcctca agaccggcgc ggcggcgatg
     3061 gcggctttcg aggccaaaat gcaggccgac ggtttcaagg gctgttaaag gcgctaaaac
     3121 caaattcctt taaccacggc ttgcacgagg cggtgcatgg cgaagtggcg acaaatcacg
     3181 gagagcatcg agacgctgga aggcctggcc ttgcgggacg atcttgttgc cgagcttgaa
     3241 cagcgccggg cacgatcacc ttctgcgaag tccggcattc atttttatac gcccaccttc
     3301 aaatcgtatc agacctcgga aatcaatggc tgcggtcgca acgcctggcc ggcggtttcc
     3361 attaccggtt ccgattgcaa gctccagtgc gatcactgca aggcgaaaat cctcgagccg
     3421 ttgatcgcgg cgcgcacgcc ggagcaattg tggacgacag tggaagacct cgtcgccggc
     3481 ggcgcgcagg gcatgctgct cacgggtggc tccaatcatc gtaacgaagt cgagtacgat
     3541 ccctattatc ccgttgtccg ccgcatcaag gatgcctggc cgtcgttcaa aatcgccgtg
     3601 catacggcgt tggtggatgc cgcgcaagcg caacgaatgg aggatgccgg catcgacgtg
     3661 gccatgatgg acgtgatcgg cgcgcaggag acggtcacgc aggtctatca tctcaagcgc
     3721 ccggtcgagg acttcgagcg cacgctcgaa acgctggtgg ccacgaagat gaaagtggtg
     3781 ccgcatatcg tcatcggcct gcattacggc cgcctgcttg gcgaatggaa ggcgctcgat
     3841 atgatccggc gtcacaagcc cgatgcgctg gtgctggtgg tggtcatgcc catgtatgcc
     3901 acacccaagc ggccttttgt cacgcccgat ccgcacgagg tcggccggtt tttcctggag
     3961 gtgcgcgagg cactgccgga catcccgttg ctgctcggct gtgcccggcc gccgggggca
     4021 gccaagaccc tcatcgacac ctacgcggtc atggccggcc tcaacggcat ggcccatccc
     4081 tccgatggca tggtcgagct ggcggcgcgg ctggaacgcc cggtgcgcgt gacgccggcc
     4141 tgctgctcca tcgccgtggg cgatgaggtc atggcgctcg aatcggagga ccagagtctc
     4201 acactcgata tccagaccat cctgcagcag gagcagcagc ggcgtcgcct caaaggaatc
     4261 ccggtgatca gtaaagccgc cgccggaggc tgccgcggca catgattcct tcttcccgcg
     4321 agccttggcg cctgatcgac accggcctgc ggccggcggc ggagaatatc gcgatcaacc
     4381 gcgccatgct cgaagcgcat caggaaggtc tgatcccgca cacgctgcgt ttcctgcgct
     4441 tccagccgag cgcgctcgtc ggttttcatc aaagcgtcga gcaggaactg cgcgcggatt
     4501 attgcgccgc gcacgggatc accatccagc ggcgcatcac cggcggcggc gcgatctatt
     4561 tcgatgagac ccagatcggc tgggagttgt atctgcaccg gcgcgtgctg ggcacggccg
     4621 atatgcagga aatctcccgg cgcatctgcg agatggcggc cgtggggatt cgctgtctgg
     4681 gcgtagacgc gcagttccgc ccgcgtaacg acatcgaggt caacggccgg aaaatctccg
     4741 gcaccggcgg cgccttcgag ggcgattcga tcatgtatca gggaacgctg ctgattgatt
     4801 tcgacatcga gaagatgctg cgcgtgttgc gcatccctgc ggagaaattg tccgacaagg
     4861 ccatcgcctc ggcgcgcgag cgcgtggcca atctgaagga tctgctcggt agcctgcctg
     4921 atatggaaac ggtcaagcag tctctgaccc agtcctttgc cgagggcttc aatgtcgagt
     4981 tcgagccggt tgctgccctg agcgcacccg agcaggagaa attccatcag gcgctggcgg
     5041 agatcggtca tcccgactgg atcaatctgg tgagccggcc attaaccgat gcgccgatgc
     5101 tcgaaggtct gcacaaagcc gatggcggtc tgctgcgcgt ggcgctcacc gtggacaggc
     5161 cggccaaccg gatcaagcag gcctggttca cgggcgattt cttcgtcaac cccaggcgca
     5221 tggtcctgga cctcgaggct gcgttgcgcg attcgccgct ggcagactat cgccataccg
     5281 tgaatgaatt tttcagacat tatccggttg agatgctgcg gctacgcccg gaagattttt
     5341 cccacacgat cgaaaacgcg ctggctgcgt ttgtcgcaga ctccgttccc gttgctggag
     5401 gcgtgacgcc atgacccgga cggtggatgt gctggtagtg ggcgccggtc cgggtggctc
     5461 ggcggcggcg tggcgcgccg cgaaagcggg atgccgggtg ctggttgtcg agaaaaagaa
     5521 acgtatcggc gagccggtgc aatgcgcgga attcataccc ggcccgatgg tggattacgt
     5581 gcgcgacgat ggtgtcctgg cccagcatat cgagggcatg aaaacctatc tcccttcagg
     5641 gaccgtgtcg ccgtcgcctt ttcccggcct catggtggat cgcgccgcct tcgatcgcgc
     5701 cttggccgcg cgcgccgagt cagcgggagc ggaaatcatg gccaacaccg tgctggtggc
     5761 cctggatgcc tcgcagcggg tggcgcacct ccgccatgat gaccggacca gcgatattca
     5821 ttatcaaatt ctggtcgccg ctgatggtcc ccattccaca gcggcgcgcc tgctgggatt
     5881 gatgcccttg cccgtgatcc agacccgcca atacaccgtg ccgctgaaag cggcctatgc
     5941 cgataccgat atctggctgt ccgatgaatt tcccggcggc tatggctggc tgttccccaa
     6001 gggagcgctg gccaatctgg ggctcggcat tgaccgctgc ttcgagccgg acctgaaaac
     6061 accattggat catctgcatg aagaactcat caggcaaggc agggtgggat ccgagatcgt
     6121 tcagcgtacc ggcggcgcca ttccggtggg tgggctgcgc gagcggcttt cgtacaaaaa
     6181 taccctttat gtcggggacg ctggcggatt tacgcatccc attaccggcg ctggtattgc
     6241 cgcggccgtg caatccggtg agcgcgccgg actggccgcg atggaatata tgaatggtgg
     6301 cgtccgggcg ctggaaaact atgacgaaga catgcatgaa cagtatgggc ccacgctgac
     6361 acgggcgctg gcacggcgcg cggaactcgc acattactgg cgtggcgcgc gggccaatga
     6421 tgatccggtc cagcgccgtg gctggatcgc cttcaatgaa tattttgcct catgagaaac
     6481 aaggagatac gatgagcgcc gctgccgata ccgattcgat acccgttacg ttctatcgtc
     6541 cgaattacca tatcaaaacg ccggcgatgc gctcaccgga atacgtgcag ctatccacgg
     6601 ccgcggccat caccctgggc ctgatcgacg ggcatatgca ccgcacggct tgcacgcatt
     6661 gcctgaatct cctcgtaact tatccggaag gttgtcgcgc caattgcgcc tattgtggat
     6721 tggcacgtca ccgggaagag acgcgtgatt atgccgaccg caattttatc cgtgtcgact
     6781 ggccggcggt gcaatacgat gaggttattg cgcgcgtcaa aaagggcgag gataaggacc
     6841 agttcgagcg catgtgtatt tccatgatca gccatcccga ttccaatgac gatactttta
     6901 tacttctgga gaagtgggtg cgggaggtgc cgcaaatccc ggtttcgatc ctttccaatc
     6961 ccacgacgct ggattatgac gatctggtgc gcatgcgcga cctgggcgcg gatattttta
     7021 cggtggcgct ggatgcggtg acgccggaga tcttcgaccg cacccgtgga aagggcgtgg
     7081 acagcccgca caaatgggaa aaatactggc gcacccttga atgggccgct gaaatctacg
     7141 gcccggagaa atttggcgca caccttatat gtggcatggg cgagaccgaa caggaaatcc
     7201 tcgatgtggc gcagaagatt cgcgacatgg gcggacacaa tcatatgttc gcgtttttcc
     7261 cggaacgcgg ttcgctgatg gaggactggg agccggtgcc gcgcgaccaa tggcggcggg
     7321 tgcagctggc gcgatacctt atagattacg ctgacgggca cgtcagtcgc atgcggttcg
     7381 atgcttccgg acgcttggtg gattacggat tcgataagga tgccctggaa gcggttatgc
     7441 aatccggcaa accgttccag acttcgggtt gccccggtcg ccagaacgat atctcggcct
     7501 gcaaccgtcc ttacggcgat tccatgccgg cggatattcg atcgttcccc ttcgcgctgg
     7561 atgcgcgcga cattgaaagg gtgcgacgcc agatgttagg cgagaatatc tgcacttatg
     7621 aagaatgagc gggatatgtc tcagcattac agtctttaac tctcccttga cccactatta
     7681 gaaatcacta atattcatcg agttacccct atttcttact gtttacatta agtatttgac
     7741 atttctccgg ggtcaacttt acttcgacca taagtgtgga tgttgcactt cagggacgtt
     7801 agttgcggta acaacaatga ttttgccttc gcaaccaacg ggactcttaa gcgtacctgg
     7861 acaggaatat cggacgagcg aaaaagctcg cccgcccgga ttcagattca ggaacaaggt
     7921 taagcagtct ttcggggcga gcagctctca actttttcgg aggaagatga catgtcgggc
     7981 atgaaacgga atttggtatt gtctttggcc gtcgccgccg ccgtggcggc gccaactgcg
     8041 tttgcgacaa acggctattt tcagcatggt tatggcatga agagctccgg catgggcggc
     8101 gtttccaccg ccatgacccg cgacgccttc ggcggcgcca acaatccggc caccatggtc
     8161 tgggtcggta accgtatgga catcggcgtg acctggttca accctgaacg ctcagcatct
     8221 cgcagtggag cgttggatac gacgatgaat ggatcagccg atagcgatag cactgatttt
     8281 ctgatcccgg aatttggtta taaccacatg ttgaatccga acatgtcatt tggcgttact
     8341 gtttatggta acggcggtat gaatactgat tatccgggtg gtacacttcc gctgactggt
     8401 ggtacatgcc aaggtttcaa cccggcaggt gcgggtggtg cgccttacaa cctgctttgc
     8461 ggttcgggaa gtctcggtgt tgatctgatg cagcttatca ttgcgccgac ctggtcatac
     8521 aagttcaacc agaacagctc cgtgggcgta tcaccactgt tgggttatca gcgcttcaag
     8581 gcagagggta tccagtcttt ctctggtttc tcgactgatg ctaccaagct aaccaataat
     8641 ggctatgaca gcgcgaccgg tgtcggtttg cgtctcggct ggtatggccg gatgagcgat
     8701 gcggtgtcgc tcggcgcggc ctactcgacc aagatcgcca tgagcaagtt cgacaagtac
     8761 gccggtctgt tcgcggaaca gggcgatttt gacattccat cgaactacaa tctgggcgct
     8821 accttcaagg tcggcccaac tgtgttgctc ggtattgatt atcagaaaat caagtacacc
     8881 gacgtcaaat cggtttccaa tccgagcgac tatggccttg ggggtgctgg tattgctaac
     8941 tcactcggtg gtagtggtgg ccgtggtttc ggttggggtg atattgacgt ttggaagatc
     9001 ggtgtggaat accagcacag taaggacctt gtcctgcgtg caggcgtaga caagtctgac
     9061 aatcccatta aatcaagcga cgtgaccttc aatatcctcg cacccggtgt cgtggagaac
     9121 cactattccg tggggttcac cctggcaatg gataagaaga gcgagctgac cggtgcgttt
     9181 acctatgtgg ccgaaaacaa ggtcagcgga tcgagcctct tcaataactt tactggcggc
     9241 acttccggca ccgaagaaat caagatgcac cagaacgtac ttggcattgc ctggagcaat
     9301 aagtggtaag tgaagtaagt atttaagtcc gtaattgaag ggctggaagc aattccagcc
     9361 cttttgttta aaaccggagg tgagcgtcat gtggaaaaat tacataatca aatctcttgt
     9421 acttggtgtc actctcttat gggtgacatc gcaggcagcg gcagaagcgc tcaaaccttt
     9481 tatattgggc gatgcgcttc ccggcgatgt gaaacaggtg tcggagctgg tcaagacgaa
     9541 actgacggcg cagggttttg aggttgtcgg gtcctattcg ccttttcccg gagccatggt
     9601 catctgtgtc accaataacg atctgaaaac ggctgcggcc aaggcattca acggcggatt
     9661 cggcgcggcc cagcgtgtcg cggtgaccga tgtaaaggga aaaatccagg tgtcatatgt
     9721 caatccggcc tatatcggca cggcctacgg attgggcaaa ctcgaaggcg tttccgccaa
     9781 gatgaaaacc gccttgggtg ccgtgaagcc gttcggttcg gcaaacggca ttgaagaaga
     9841 aagactagta ccgggccagt accgttatgc catgttcatg ccctatttca aggatgtgga
     9901 tattctgaag gaacacccgg attacaaaac tgcggtggaa accgtggaaa agaatctggc
     9961 cgccggtctt ggcggaacca agaaggttta tcggatagac atcccgggca agcaggtcag
    10021 cgtattcggc gtggcgattc ccaagggtga cggccctgac aagggcaaca aggataccga
    10081 caaggaaatc atcgacatcg ttgactatgg cgacctgaag cacacggcgt atctgccgta
    10141 tgagttgatg gtcgacggca accgtgtgat cgcgatgcgc gcccgttatc gcattgcgct
    10201 gaatttcccc gacaccaaaa tggccggcga acacggcttc accaagatca tgaccgcgcc
    10261 atggggaatc caggtcgccc tcgaagctgt cgcgggattc aagcgcgaac tctaacagcc
    10321 tttctaggcc tcccggctga atttgccggg aggccagttg gcctcaaaaa aagacacgcc
    10381 tcggcgtgtc tttttttgta tggcaatacc cgggattcag cgtccacttg atcagatggt
    10441 ttggtcgcgt aaagggcaga atgatctaaa ctagacacag aagacataca tcaaggtaat
    10501 catgtccgaa tccgctaata cgaacatcgg cgccaatccg gcagaaagcg ccggcgcgac
    10561 cgccacgatc agcagcacaa ccgacccgct gaagctgctc agccaggtgc tggcgcatgc
    10621 ggtgcgcgtc aatgcttcgg atattcattt gcgcacgcgt aatcatccga ttctgcgcgt
    10681 ggacggcaat ctgcgcgccc tgcgcgagat tccgccgctg ccgcccgaat tcatggaacg
    10741 gctgtcgcgc accatgatgt cggcccgctt gctggccata ttcgagaaat cccatcaggt
    10801 ggacctctcc atcggtttca aggacatcgg ccgtgtgcgc gccaacatct tcttccagcg
    10861 cggctcgatc ggcatggtgt tgcgcgtcat cagcaccaac attcccacgc ccgaggaatt
    10921 gtttcttccc gacatcgtga tggcccttac gaaactggaa cgtggactgg tattgctcac
    10981 cggcgccacc ggttccggca aatccacgac actggcctcg ctcatcaaca gcatcaatct
    11041 ttcgcgcagc gaacatatca tcaccatcga agacccggtc gaattcctgt tctccgagaa
    11101 gcgctcgatc atcacccagc gtgaagttgg catcgacacc aacacctttg ccgaagccat
    11161 gcgcgctgcc ctgcgtgaag acccggacgt gattctgctg ggcgaaatgc gcgacccgga
    11221 aaccattgaa accgccatga cggcggcgga aaccggacac ctggtgttct caacggtgca
    11281 ctcgccggcg gcggcggaaa cgatctcgcg catcgtcacc agctttccgc cggaggcgca
    11341 ggtcggcgtc cgcgccaagc tcgcccagaa cctgcgtggc gtggtaagtc agcggttgtt
    11401 gccgcgcaag aacaaccagg gccgtatcgt ggcctgcgag gtcttgacgg tttcggcgct
    11461 cgcgcgcgaa tatatcctcg acccgctcaa gatcaaggaa ttgggcgacc tcatccgcaa
    11521 gggcaccatg gccgagggca tgctgagttt cgacacctcg ctgcttaatc tggtaaagca
    11581 gggccacatc gacgatgaaa ccgccctgtt ctatgccacc agcccgactg accttaagct
    11641 caaactggaa gggttcgcct agcagctatc tcaaaacctg ctgcgcgctg cgctcctttc
    11701 ccgctgcgcc atccggctcc gcgggaaagt ccggggccgc cgtccctggc ggcccgttcg
    11761 gtggcccgaa cgtccactgg acgttccggg aaagctccgc ctcaccctca cccggctgac
    11821 ggccgctcgc gacgtttttg agatagcttc tagactcgcc aaccggcacg ccctcgcggg
    11881 ataaatccac tgccagccgg accggctttg agttaagcgc gctgtcctac ataatccacg
    11941 tttccaagat gaataacggc ctcccg
//
LOCUS       DBNGZE010000052         2389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1813480, whole genome shotgun sequence.
ACCESSION   DBNGZE010000052 DBNGZE010000000
VERSION     DBNGZE010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1813480"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="dxr"
                     /locus_tag="AC16FV_01335"
     CDS             <1..857
                     /gene="dxr"
                     /locus_tag="AC16FV_01335"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR2848737.1"
                     /translation="GKRVLLANKEPLVMSGHVFIEEAQRANAELLPVDSEHNAIFQCM
                     PDGFRAGVPAPAVRRIFLTCSGGPFRATPAESLAAVTPEQACAHPNWVMGRKISVDSA
                     TLMNKGLELIEACWLFGVTPDQVEIVIHPQSVIHSLVEYVDGSVLAQLGNPDMRTPIA
                     HALAWPERIESGVKSLNLLEVARLDFSAPDQERFPCLRLAYEAARGGGTMPAILNAAN
                     EVAVQAFLERRVGFTDIPRVIEHTLSSLPASHETHLDAVLSHDRRARDVAGSFIAARE
                     PRIGQGVS"
     gene            854..2215
                     /gene="rseP"
                     /locus_tag="AC16FV_01340"
     CDS             854..2215
                     /gene="rseP"
                     /locus_tag="AC16FV_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR2848738.1"
                     /translation="MMDLLYYAGAFVVALGILITVHEFGHFWVARRLGVKVLRFSVGF
                     GKPLWSRRVGKDNMELAIGAFPLGGYVKMLDETEGEVPAVDAHRAFNRQRVWKRMAIV
                     IAGPLFNFIFAILAYWAVYMIGVDGIQPVVGKVVEGSIAQQAGFNAGDTILSIDGKPV
                     QSWDQRRLYVFQRALDRARVRVEVRDAQGQVKRLELDLSSLPVQEVNSSLFERGIGLM
                     GYFPEALPVIGAIEAGPAMRAGMEAGDRLVEVNSQPVGTWEELVKLVSGNPGKSIHIV
                     VDLNGKRLDFDVTPDVVEQGDKTIGRINIRPQFSEIPDEMRVRVRYDSLAALTEGIGN
                     TWRMSALTLEMLYRMLKLEVSTRNISGPITIAQYAGYSAKVGAEQFVLFLAVISISLG
                     VLNLLPIPVLDGGHLMYYIIEAVKGSPLPESVLVWGQQIGVALLVGLMVLAFYNDLTR
                     IFL"
     gene            2256..>2389
                     /locus_tag="AC16FV_01345"
     CDS             2256..>2389
                     /locus_tag="AC16FV_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR2848739.1"
                     /translation="MKRFFAALLLGLVCMSAGQAMESFVIKDIRVEGLQRISAGTVFN
                     "
BASE COUNT          428 a          651 c          780 g          530 t
ORIGIN      
        1 cgggcaagcg cgtgctgctg gcgaacaagg aaccgctggt gatgtccggc catgtcttta
       61 tcgaagaggc gcaacgcgcc aacgccgagc tgttgccggt ggacagtgag cacaacgcca
      121 ttttccagtg catgccggac ggtttccgcg ccggtgtccc ggcacctgcc gtgcggcgta
      181 tttttctgac ttgttcgggc gggcctttcc gcgctacgcc ggcggagtcg cttgccgcgg
      241 tgacgccgga gcaggcctgc gcccatccca attgggtcat gggccgcaag atttcggtgg
      301 attccgcgac attaatgaac aaaggtctgg agctgatcga ggcctgctgg ctgttcggtg
      361 tcacgcccga tcaggtggag atcgtcatac atccgcagag cgtgattcat tcactggtcg
      421 agtatgtcga cggttcggtg ctggcccagc tgggcaatcc ggacatgcgc acgccgatcg
      481 cgcatgcgct ggcttggccg gagcgtattg agtccggcgt gaagagtctc aacctgcttg
      541 aggtagcacg cctggatttc agtgcgccgg atcaggagcg gtttccgtgc ctgcggctgg
      601 cgtatgaagc ggcgcgcggg ggcggcacca tgccggcgat cctgaacgcg gccaacgaag
      661 tggcggtgca ggcctttctc gagcggcgcg tcggatttac cgacatcccg cgcgtgatcg
      721 agcatacgct ctcaagcctg ccggccagtc atgaaactca tctcgatgcc gtgttgtctc
      781 atgacagacg tgcccgcgac gtggcgggca gcttcattgc ggcgcgggaa ccgcgcatag
      841 ggcaaggtgt ttcatgatgg atctgcttta ttatgccggc gctttcgtgg tggccctggg
      901 catcctgatt acggtgcacg aattcggcca tttctgggtg gcgcgtcgtt tgggggtcaa
      961 ggtgttgcgg ttttccgtcg gtttcgggaa acccctgtgg tcgcgtcgcg tgggcaagga
     1021 caacatggaa cttgccatcg gggcgtttcc gctcggcggt tacgtgaaga tgctggatga
     1081 gaccgagggc gaggtgccgg cagtggatgc gcatcgggcc ttcaaccgtc agcgtgtctg
     1141 gaagcgcatg gccatcgtga tcgcgggtcc gttgttcaat tttattttcg ccatcctggc
     1201 gtactgggcg gtttacatga tcggcgtgga cggcatccag ccggtagttg gcaaggttgt
     1261 cgaaggttcg atcgcgcagc aggctggctt caacgccggt gacaccatcc tgagcattga
     1321 cggcaagcca gtgcagagct gggaccagcg gcgcctgtat gtatttcagc gcgcgctcga
     1381 tcgcgcgcgc gtgcgggtgg aagtacgcga cgcccaaggc caggtgaaac gtttggaact
     1441 ggacctgtcg agccttccgg tgcaggaggt caattcatct ttattcgaac gcggtatcgg
     1501 tttgatgggg tattttcccg aggccttgcc ggtcataggc gcgatcgaag caggacccgc
     1561 catgcgcgcg ggaatggaag ccggcgatcg cctggtggaa gtcaattccc aacccgtggg
     1621 cacctgggaa gaactggtca agctggtcag cggcaacccg ggaaaatcca tccacattgt
     1681 tgtggatctt aacgggaagc gactcgattt tgacgtcacg cccgatgtcg tcgagcaggg
     1741 agacaagacg attgggcgca tcaatatccg tcctcaattc agcgaaatac ccgatgagat
     1801 gcgggtgcgg gtgcgctatg actcgctcgc ggcattgacg gaagggattg gcaatacctg
     1861 gagaatgtcc gcgctcacgc tcgagatgct gtaccggatg ctcaagcttg aagtgtccac
     1921 gcgcaacatc agcggcccga tcaccatcgc gcagtacgcc ggctattccg ccaaggtcgg
     1981 tgccgagcag tttgtcctgt tcctggcggt catcagcatc agcctgggtg tgttgaatct
     2041 cctgccaatt ccggtgctcg atggcggaca tttaatgtat tacattatcg aagccgtgaa
     2101 gggcagtccg cttcccgaga gcgtgctggt atggggccag cagatcgggg tggcgctgct
     2161 ggtcggcctg atggtgctgg ctttttacaa tgatttgacc cggattttcc tctaaacgat
     2221 cgccgctcgc ccgaatccag aaaaattcct gacgaatgaa acgattcttc gcggcacttc
     2281 tccttggact tgtctgtatg tccgccggtc aggcgatgga gtcctttgtt atcaaggaca
     2341 ttcgcgtgga gggtttgcag cgcatttcgg cgggcaccgt gttcaatta
//
LOCUS       DBNGZE010000053         2418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1813501, whole genome shotgun sequence.
ACCESSION   DBNGZE010000053 DBNGZE010000000
VERSION     DBNGZE010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1813501"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="AC16FV_01350"
     CDS             <1..1665
                     /locus_tag="AC16FV_01350"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR2848740.1"
                     /translation="DGRDVLGIRLNCHKRYITLGPVATVLGLAFKLRDPDHLLGTNED
                     IGITVALVPTHLPGVDIGRRHFPALNVFQNGPTWGRDVFLPMDHVIGGVEQVGKGWKM
                     LMSALAAGRGISLPSLSAAGAVYTAHTTGAYARIREQFHISIGKFEAILERLGRLAAT
                     AYLLDAARRMTCAALDAGHTPAVVTAIMKSQATERLRVSVNDAMDVHGGKAIMEGPQN
                     YLGSLYRAVPIGITVEGANIVTRSLIQFGQGSIRSHPYILREMLALEDDDHARGLDAF
                     DRAFWAHVGHAIANAFRAWGRSWSGGLFAPAPNAGKTTQFYRQLSRYASAFALSVDMT
                     LLTLGGDLKRKEMISARFGDLLSELYLLSAVLKRWQDEGRQDADLPLVEWCMHSGMLT
                     IATRFDEILANFPNRLVAGLLRLIIQPLGPRRRGPSDSLTRACATLILEPSATRDRLT
                     VDLFHPKDDSGLARLEIAFKRTAAAQSLRDRINKAHVHDIDQALKQNLIDASEAAQLK
                     ETAQAVALAIAVDDFTAEELTHHDADHAQGDKPSPAQKPPRPAAAE"
     gene            1662..>2418
                     /locus_tag="AC16FV_01355"
     CDS             1662..>2418
                     /locus_tag="AC16FV_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR2848741.1"
                     /translation="MNDRPVYIIDGSRTPFLKARGVGPFTPVDLAVQCGRPLLLRQPF
                     APDAFDKVILGCVNVIADEMNPARVAALRLGTGEAMTACTVQINCGSGMQSIETGYRD
                     IQDGVSDLILAGGTEALSYAPLVFRRSAVEWYMKLFSARNVWSRLKAFASIRPSFFKP
                     IIGLERGLTDPIVELNMGQTAEVVAHLFKVTRRNADQYAAESQQRLTRAQKDKIFDGE
                     MEPAFGHDGKVYDFDDGVRPESTVDSLAKLKPVF"
BASE COUNT          486 a          771 c          685 g          476 t
ORIGIN      
        1 gatggccgtg acgtgttggg aattcgcctg aactgccata agcgatacat cacgctcgga
       61 ccggtcgcga ccgtacttgg cctcgccttc aaactgcgcg atcccgatca tctgcttggt
      121 acgaacgaag acattggcat tacagtcgcg ctggtgccga cgcatcttcc cggcgtggat
      181 atcgggcgcc gacattttcc cgcgctcaac gtcttccaga atggcccaac ctggggacgc
      241 gatgtcttcc taccaatgga tcacgtcatc ggcggcgtcg aacaagtcgg taaaggctgg
      301 aagatgctga tgagcgcgct ggccgccggg cgcggaattt cactgccgtc attgtcggct
      361 gctggcgccg tctacaccgc ccacaccact ggcgcctatg cgcgcatccg cgaacaattc
      421 cacatttcga tcggaaaatt tgaagccatc ctggagcggc tcggacggct agccgccaca
      481 gcctatctgc tcgacgccgc gcggcgcatg acctgcgccg cactcgacgc cggccacacg
      541 ccggccgtcg tcacggcaat catgaaatcg caagcgaccg aacgtttgcg cgtttcggtc
      601 aatgacgcca tggacgtgca cggcggcaag gcaatcatgg agggacctca gaattatctc
      661 ggcagtctct accgcgccgt gccgatcggc atcaccgttg aaggcgccaa tatagtcaca
      721 cgcagcctga tccagtttgg ccagggctcg attcgcagcc atccctacat tctcagggag
      781 atgcttgcgc tggaagacga cgatcatgcc cgcggtctcg atgctttcga ccgcgcgttc
      841 tgggcgcatg tcggtcacgc tatcgccaat gcttttcgcg cctggggacg cagttggagc
      901 ggcgggctgt tcgcgcccgc gcccaatgcc ggaaagacca cccaattcta ccggcagcta
      961 agccgttatg cctcggcctt tgctctatcc gtggatatga ccctgctgac gctaggcggc
     1021 gacctcaagc gcaaggaaat gatttcggca cgcttcggtg atctcctgtc cgagctttat
     1081 ctgttatcgg ccgtgctcaa gcgctggcag gacgaaggcc ggcaggatgc cgacctgccg
     1141 ctggtcgagt ggtgcatgca cagtggaatg ctaacaatcg ccacgcgctt cgatgagatt
     1201 ttagcgaatt tcccaaaccg tctggtcgcg gggctgttgc gccttatcat tcaaccacta
     1261 gggccacgcc gccgtggtcc ctctgacagc ctgacgcgcg cttgcgccac acttatcctc
     1321 gaaccgtctg cgacgcggga taggctcacc gtcgatctct tccatcccaa agatgacagc
     1381 ggtcttgcac gcctagaaat cgctttcaag cgcaccgcag cggcgcagtc gttgcgcgac
     1441 cgaattaaca aagcgcacgt tcacgacatc gatcaagcgc tcaagcaaaa tctcattgat
     1501 gccagcgaag ccgcgcagct taaagaaacc gcccaagcgg tcgccctcgc cattgcggtt
     1561 gatgatttta ccgccgaaga attgacgcac cacgacgccg accatgcaca aggagacaag
     1621 ccatcgccag cccagaagcc accccgacca gcagccgccg aatgaacgat cgcccagtct
     1681 atatcatcga cggcagccgt acgccgtttc tcaaggcgcg cggtgtagga ccatttacgc
     1741 cagtcgatct ggccgtccag tgtggccgac ccttgctgct gcgccaacca ttcgcgcccg
     1801 atgcgttcga caaggtcatt ctcggctgcg tcaacgtcat cgccgacgag atgaaccctg
     1861 ctcgcgtcgc cgcgctgcgg cttggtacgg gcgaggcaat gaccgcttgt accgtacaga
     1921 tcaattgcgg ttccggcatg cagtcgatcg aaaccggcta tcgtgacatt caggatggcg
     1981 tctcggacct gatcctcgcc ggcggcaccg aggctttgag ctacgcgccg ctcgttttcc
     2041 gtcgtagcgc agtcgagtgg tatatgaagt tgttttcagc tcgcaatgtt tggtcgcgcc
     2101 tgaaagcctt cgccagcatc cggcctagct ttttcaaacc catcatcggc ctggagcgcg
     2161 gcctcaccga tccgatcgtt gaactcaaca tggggcagac cgccgaggtg gtggcgcacc
     2221 ttttcaaggt gacgcggcgc aatgctgatc aatatgccgc cgaaagccag cagcggctca
     2281 cccgcgcgca aaaggacaaa atcttcgacg gagaaatgga gcccgcgttc ggccacgacg
     2341 gcaaggttta cgactttgac gatggcgtgc ggcccgagag caccgtggat agcttggcca
     2401 agctcaagcc ggtatttg
//
LOCUS       DBNGZE010000054         5027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_7740, whole genome shotgun sequence.
ACCESSION   DBNGZE010000054 DBNGZE010000000
VERSION     DBNGZE010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_7740"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="AC16FV_01360"
     CDS             <1..913
                     /locus_tag="AC16FV_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2848742.1"
                     /translation="LGMVNGMGRDRAGQLVLEQAMLPATATSEYRTRVFAWYNVLQDI
                     GHALGSLAAALPALLQAAAAVPVVASFQATLGLYALLMLACVPLYLGLSAAVELDGRH
                     ASAAVTPQSRRIVWRISGLFAIDSLAGGFLGTALLSYFFFERFGAGEAAIATLFFLAR
                     GANAVSHLGAAWLARRIGLINTMVFTHIPSSLMLVAVALVPQFWMAATLFLVRELLVE
                     MDVPTRQSYVMAVVRPEERTWASGITGLVRLGGWAVAPFAAGFLMQGLALATPLLIGA
                     GMKIGYDLLLWKSFRDLHPPEERDAPG"
     gene            951..1643
                     /locus_tag="AC16FV_01365"
     CDS             951..1643
                     /locus_tag="AC16FV_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HIR2848743.1"
                     /translation="MPIRSKRSKPRASLRKIYDLLYAANGRQHWWPGDGAFEIMVGAV
                     LTQNTAWTNVEKAIANLKKAKVLSPEAIAKAHPRRLATWLKPSGYFNIKAKRLKALCR
                     WLMEQGSVGHLAKLPTSELRHALLNVHGVGPETADDILLYAFNRPVFVIDAYTRRIFT
                     RLGLITGNEHYETLRGLFENELGFSAPLFNEYHALIVAHGKDICKKRPLCGSCSLAEK
                     CPAVMSHKSARD"
     gene            complement(1767..2720)
                     /locus_tag="AC16FV_01370"
     CDS             complement(1767..2720)
                     /locus_tag="AC16FV_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR2848744.1"
                     /translation="MDGCFQPAWWCRGPHAQTLWARLVRRAPKISFWRERLELPDGDF
                     IDLDWSESGNGPIVIVLHGLEGSSDSPYARGIMRAIEQRGWRGVVMHFRGCSGEPNRL
                     ARSYHSGDTGDLSYFVNTLRRREPRTPLATIGFSLGGNVLLKWLGKAGGAAPLRAAVA
                     VSVPYVLRNAADRLNHGFSRLYQWQLLRSLRNSVAEKRNRIKLPLKIRDLSTLSSFRD
                     FDEHVTAPLHGFDSADHYYTVSSSRQYLRGITVPTLLLHARDDPFMSEKAIPGQNELS
                     ESATLEVSVHGGHVGFVAGAYPWHARYWLDERIPTYLGQYL"
     gene            complement(2757..3191)
                     /locus_tag="AC16FV_01375"
     CDS             complement(2757..3191)
                     /locus_tag="AC16FV_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848745.1"
                     /translation="MNSSDVFYKAFRGNFSSLLSWQQLDEFWEVVRGKADAGWYIYAI
                     GTPVPTLASSTSDVLKFIHEMNALLRKEHYEDYCGIVYTDNKQDPALIKIFDPDNLGV
                     SCGFSNNPPLPGWVLSLIPPQPLETKRPLPAGRLRWWETLWT"
     gene            complement(3221..4402)
                     /locus_tag="AC16FV_01380"
     CDS             complement(3221..4402)
                     /locus_tag="AC16FV_01380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR2848746.1"
                     /translation="MEMKAEKTEIVAPVAEKVRVLVADDSRVIRKAISKILSNEFDLV
                     ESEDGETAWEQLLKDDSIEVLVSDIEMPKLDGYSLICRIRAADSERVRNVPIIVITGA
                     DDELTRERAFACGATDFIIKPIDGVQLLARTRAHAKLDQTTRKLEETATALEEQTAVD
                     PLTQLHSRRYFIQRGVQDLAYAKRHNSDLSIIRIDIDNFRTIYKKHGDQASDQILVWL
                     AKILVATCRTEDTVARIGGGEFAIIAPSSGRMEAAVLCERVRTAVAAEPFKHENLSLP
                     LTVSLGLSTLGRDPGETIEELLALAEQRMTLAKAAGGNRLGVSYQEEAPKPEEAVIEQ
                     PDLETALKMLGANEGGKLVPFLPDLINRCIPLLEFCNKKLDLGLGFAIESLKDKLSDM
                     R"
     gene            complement(4582..>5027)
                     /locus_tag="AC16FV_01385"
     CDS             complement(4582..>5027)
                     /locus_tag="AC16FV_01385"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR2848747.1"
                     /translation="GGMDIIWIIIVLVSAAICGDNTNYWIGQHLGPRVFRRHDSRFLN
                     PRHLERAQEFYARHGAKAVIIGRFLPIIRTFVPFVAGIGRMPYLRFLSFSILGTFLWV
                     LSFVLAGYFFGNIPAVKRNLTFVVFAIIVISILPGAVQYWRSRRA"
BASE COUNT          981 a         1456 c         1563 g         1027 t
ORIGIN      
        1 cctcggtatg gtcaacggca tggggcgcga ccgcgccggc cagctcgtgc tcgagcaggc
       61 catgttgccg gcgacggcta ctagcgagta ccgcacccgg gtattcgcct ggtataacgt
      121 gctccaggat atcggccacg cgcttggcag tctggcggcc gcgctgcccg cgttgctgca
      181 ggccgcggcg gcagttcccg tggtcgcaag tttccaggcc acgctcgggt tgtacgcgct
      241 gctgatgttg gcgtgcgtgc cgctctacct cgggttgtcc gcggcggtcg agcttgacgg
      301 ccgccacgca agcgcggccg tcacgccgca gagccggcgg atcgtgtggc gcatctcggg
      361 gctgttcgcg atcgacagtc tcgccggcgg ttttctcggc accgcgttgc tgtcgtactt
      421 cttcttcgag cgcttcgggg cgggggaggc ggcgatcgcc acgctgttct tccttgcgcg
      481 cggcgccaat gccgtctcgc acctcggcgc cgcttggctt gcgcgccgca tcggactcat
      541 caataccatg gtgttcaccc acatcccctc gagcctgatg ctcgtggccg tggcgttggt
      601 gccgcaattc tggatggcgg cgacgctgtt cctcgtgcgc gagttgctgg tcgagatgga
      661 cgtaccgacg cgccagtcct acgtcatggc cgtggtgcgg cccgaggagc gcacctgggc
      721 ctcgggcatc accggtctgg tgcgtctggg cggctgggcg gtggcgccgt tcgcggccgg
      781 gtttctcatg caggggttgg cgctcgcgac gccgcttttg atcggcgccg gcatgaagat
      841 cggttacgac ctgctgctgt ggaaatcctt ccgcgacctg catccgccgg aggagcgcga
      901 tgcgcccggc tgatgtcgta gaatatggcg aacttcggaa tcttgttgac atgccgatac
      961 gctccaaacg atcgaagccg cgcgccagcc tcagaaaaat ctacgatctt ctctacgccg
     1021 ccaacggccg gcagcactgg tggccgggcg acggcgcgtt tgaaatcatg gtcggggcgg
     1081 tactgacaca aaacaccgct tggactaacg tggaaaaggc gatcgccaat ctgaaaaagg
     1141 ccaaagtgct gtcgcccgaa gcaattgcaa aagcgcatcc ccggcgtctc gctacctggc
     1201 tcaaaccctc cggctatttc aatatcaagg ccaaacgcct gaaagccttg tgccgctggc
     1261 tgatggaaca gggcagtgtc ggccacctcg cgaaacttcc cacctctgag ctgcgccatg
     1321 cgcttttgaa cgttcatggc gtcggcccgg aaacggccga tgacatcctg ctctacgcct
     1381 tcaaccgacc ggtgtttgtg attgatgcgt acacgcggcg tatttttacg cgcctcgggc
     1441 tgattaccgg aaatgagcat tacgaaacct tgcgcggcct gttcgagaat gaactcggat
     1501 tttcggcgcc gctgttcaac gaataccatg ccctcatcgt tgctcatggc aaggatatat
     1561 gcaaaaaacg gccattgtgc ggaagctgtt ccttggctga aaaatgcccc gcggttatgt
     1621 cgcataaatc tgcaagagat tgacaccacc cttgttcaga caattgcgct gatggccgtg
     1681 gtggtcgttc tttgctggtg gggtttcgcg ctctacacct ggcggcggcg cgattgtttt
     1741 gaaagataac aggtatccgc tgctcgttac agatactggc caaggtaggt tggaatgcgt
     1801 tcatccagcc aataacgcgc gtgccacgga tatgcacctg ccacaaagcc cacatgcccg
     1861 ccatgtaccg aaacttccaa agttgcggat tcggaaagtt cattttgccc ggggatggcc
     1921 ttttcgctca tgaaaggatc gtcacgcgcg tgcaacagga gcgtgggaac agtgattccc
     1981 ctgagatatt ggcgcgaact cgacacggtg tagtaatgat cggcgctgtc aaaaccgtgc
     2041 aggggagcgg tgacatgctc gtcgaaatcg cgaaaactgc tcagcgttga cagatcccgg
     2101 attttgagcg gcagcttgat acgattgcgc ttctcggcaa cgctattgcg cagggaacgt
     2161 aacagctgcc attgatacag gcgcgagaaa ccgtgattca gtcggtcggc ggcattgcgc
     2221 aacacatatg gcaccgacac cgctactgcc gcgcgcaacg gcgctgcacc accggccttg
     2281 cccagccatt tgagcagaac gttcccgccc agcgagaatc cgatcgtggc cagcggcgtg
     2341 cgcggctcgc ggcggcggag cgtgtttaca aaatacgaca gatcaccggt gtcgcccgaa
     2401 tgatagctgc gcgccagccg gttgggttcg ccgctgcagc cacggaagtg catgacgacg
     2461 ccgcgccaac cgcgctgctc gatggcgcgc atgatgccgc gggcataggg cgagtcggac
     2521 gagccctcca gaccgtgtag aacgatcacg atgggtccgt tgccgctctc cgaccagtcg
     2581 agatcgatga agtccccgtc ggggagttcc aggcgttcac gccaaaagct gattttgggc
     2641 gcccgccgca ccaggcgcgc ccacaaggtc tgcgcgtgtg ggccgcggca ccaccatgcg
     2701 ggttgaaagc atccatccat ggaagagaat ttgttcttat ctgtgtggag ttggcatcag
     2761 gtccagagcg tctcccacca gcgcagccgg ccggcgggca aggggcgttt ggtctccagt
     2821 ggttgcggcg gtatcaggct cagcacccag ccgggcagcg gcgggttgtt gctgaaaccg
     2881 caggagacgc cgagattgtc gggatcgaag attttgatca gcgcggggtc ctgtttgttg
     2941 tcggtgtata caatcccgca gtaatcctcg taatgttcct tgcgcaggag tgcattcatt
     3001 tcatgaatga atttcaggac atcgcttgtt gatgaggcca gcgtgggaac cggcgtgcca
     3061 atggcataga tgtaccagcc ggcatcggcc ttgccgcgca ccacttccca gaactcgtcg
     3121 agctgttgcc agctcagcag gctgctgaaa tttccacgga aggccttgta aaacacgtca
     3181 ctgctgttca tggtctctac cggtcacacg gatagagcca ttatcgcata tcggagagct
     3241 tgtccttaag cgattcgatg gcgaagccca gacccaggtc gagcttcttg ttgcagaatt
     3301 ccagcagggg gatacagcgg ttgatcaggt caggcagaaa gggcaccagt ttgccgcctt
     3361 cgttggcgcc gagcatcttg agcgcggttt cgaggtcagg ttgttcgatg acagcctctt
     3421 cgggcttcgg ggcctcttcc tgatagctga caccgaggcg gttgccacca gccgccttgg
     3481 ccagcgtcat gcgctgttcg gcgagcgcca gcaattcctc gatggtttcg ccagggtcgc
     3541 ggccgagcgt ggacagaccg aggctgaccg tcagcggcag ggacaggttt tcgtgcttga
     3601 agggctcggc ggcgacggca gtgcgcaccc gttcgcagag cacggcggcc tccatgcgtc
     3661 ccgaggaagg ggcgataatg gcgaactcgc caccgccaat gcgtgccacg gtgtcttccg
     3721 tgcggcaggt ggccacaagg attttggcga gccagaccag aatctgatcg ctggcctgat
     3781 cgccgtgctt cttgtagatg gtacggaaat tgtcgatatc aatacggata atcgacaggt
     3841 cgctgttgtg acgcttggca taggccagat cctgcacgcc gcgctggatg aaataacgcc
     3901 ggctgtgcag ttgcgtgagc gggtcgaccg ctgtctgctc ctcgagcgcg gtggcggttt
     3961 cttccagttt gcgcgtggtc tggtcgagct tggcatgcgc gcgggtacgc gcgagcagtt
     4021 gcacgccatc aatgggcttg atgatgaaat cggtggcgcc acaggcaaag gcgcgctcac
     4081 gcgtcagctc gtcgtcggcg ccggtgatga caatgatggg gacattgcgc acgcgctcgg
     4141 aatcggcggc gcgaatgcgg cagatgagcg agtagccatc gagcttgggc atctcgatgt
     4201 cggagaccag cacctcgatg gaatcatcct tcagcagctg ttcccacgcg gtttcgccgt
     4261 cctcggattc gacgagatcg aactcgttgc tcagaatctt gctgatggcc ttgcggataa
     4321 cgcgcgagtc atcggctacc agcacccgca ccttttcagc cacgggcgcg acgatttccg
     4381 ttttttccgc tttcatttcc atatcttggt ttattcaaga aatgggccaa atccagagtt
     4441 tgaccatggt tgcctgcatt tccgggccca ctaaataacc atagcgcagc catgggacac
     4501 attcaaagtc ttgaattacc ttgaatttca gcccgaatga ggcaggggca gcagttgcgc
     4561 cggcaaaacc ggcgcaaaaa actaggcgcg gcggctgcgc cagtattgaa cggcgccggg
     4621 gagtatcgaa atcacgataa tggcgaagac gacaaaggtc aggttgcgct tcaccgccgg
     4681 aatgttgccg aagaaataac cggcaagcac gaacgacaga acccacaaaa acgtgccgag
     4741 gatgctgaaa gaaagaaacc ggagatacgg catgcgaccg atgcccgcca cgaacggcac
     4801 gaacgtgcgg atgatgggga gaaatcgccc gattatcacg gccttggcgc cgtggcgtgc
     4861 gtaaaattcc tgtgcacgtt ccaggtgccg gggattgagg aatcgtgaat catgacgacg
     4921 gaatacccgg ggacccagat gctgaccgat ccagtaattg gtgttgtcgc cgcagatcgc
     4981 cgcactgaca aggacgatga tgatccatat gatgtccatg cctccgg
//
LOCUS       DBNGZE010000055         1516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1466532, whole genome shotgun sequence.
ACCESSION   DBNGZE010000055 DBNGZE010000000
VERSION     DBNGZE010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1466532"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="gcvPB"
                     /locus_tag="AC16FV_01390"
     CDS             <1..1281
                     /gene="gcvPB"
                     /locus_tag="AC16FV_01390"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR2848748.1"
                     /translation="NFGVDTGFYPLGSCTMKFNPKINEQIARLAGFAESHPLQEPDTV
                     QGNLALMSELQQWLAEIGGFAGVSLQPTAGAQGEFTGLLMMRAYHRERGDLKRTRILI
                     PDSAHGTNPASTTMAGFTAVELPSDARGNVDLEAVRVACDDTIAGIMITNPNTLGLFE
                     EHIGEVVRLVHASGGLVYGDGANMNALAGIARPGDLGFDVMHYNLHKTFSTPHGGGGP
                     GSGPVGVSAQLKEFLPGPVVEYARDVDGARYNLVMPAKSIGRVSTFFGNFGMLVRAYA
                     YIRQHGAEGLRENSEHAVLNANYLRVKLRDTFAVPFDRTNMHEFVCRGAIEGTSVRAL
                     DVSKRLLDYGFHPPTNYFPLIVPEALMIEPTETEAKPTLDAFVVAMQAIEKEMRHDPA
                     LVKGAPHETPVKRLDEVKAARELILSDQANVAND"
     gene            1300..>1516
                     /gene="gcvT"
                     /locus_tag="AC16FV_01395"
     CDS             1300..>1516
                     /gene="gcvT"
                     /locus_tag="AC16FV_01395"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336922.1"
                     /note="catalyzes the transfer of a methylene carbon from
                     the methylamine-loaded GcvH protein to tetrahydrofolate,
                     causing the release of ammonia and the generation of
                     reduced GcvH protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR2848749.1"
                     /translation="MPELQAPTELSRTPLHGLHVRLGARMVAFAGYDMPVQYPSGIIE
                     EHLHTRVAAGLFDVSHMGQITLRPRSGR"
BASE COUNT          295 a          466 c          455 g          300 t
ORIGIN      
        1 aatttcggcg tcgatactgg gttctatccg ctcggctcct gcaccatgaa gttcaacccg
       61 aagatcaatg agcagatcgc gcgcttggcc ggcttcgcgg agtcgcatcc gttgcaggag
      121 ccagacacgg tgcagggcaa cctcgcgctg atgtccgagc tgcagcagtg gctggccgag
      181 atcggcggct ttgccggcgt atcgctgcaa cctacggccg gtgcgcaagg tgaattcact
      241 gggcttttga tgatgcgcgc ctatcatcgc gagcgcggtg atttaaagcg gacgcgcatt
      301 ctcatccccg actccgccca tggcaccaac ccggcttcga ccaccatggc gggctttacc
      361 gcagtggaac tgccgtccga cgcacggggt aatgtcgacc ttgaggcggt acgcgtcgcc
      421 tgtgacgaca ccattgctgg catcatgatc accaatccca acacgcttgg cctgttcgag
      481 gagcacatcg gagaggtagt gcggctggtg catgcgtccg gcggtctcgt ttatggcgat
      541 ggcgccaaca tgaatgcgct cgccggcatt gcgcgtccgg gtgatctagg tttcgacgtc
      601 atgcactaca atttgcataa gaccttctcc acaccgcacg gcggcggcgg ccccggttcc
      661 ggtccggttg gagtgtcggc gcagctcaag gaattcctcc cagggccagt ggtcgaatac
      721 gcgcgcgatg tggatggtgc acgctataac ttggtgatgc cagccaagtc gatcggccga
      781 gtctccacct tcttcggcaa ttttggcatg ttggtgcgcg cttacgccta catccgccag
      841 catggcgcgg agggactgcg tgagaattcc gagcacgcgg tgctcaatgc taattacctg
      901 cgcgtcaagc tgcgtgatac atttgccgtg ccgttcgatc gcaccaacat gcacgaattc
      961 gtttgccgcg gcgcaatcga gggaacaagc gttcgcgcgc tcgacgtgag caagcggctg
     1021 ctcgactacg gctttcatcc gccaaccaat tattttccgc tgattgtgcc agaggctctg
     1081 atgatcgagc cgaccgagac ggaagccaag ccgaccctcg atgccttcgt cgtagcaatg
     1141 caagccatcg aaaaagagat gcgacacgat cccgcgctgg tgaaaggagc cccacatgag
     1201 acgccagtca aacggctcga cgaggtcaag gccgcgcgcg aactgatact ctctgatcaa
     1261 gccaacgttg ccaacgactg acgcgaggca aggcagcaaa tgccggagct acaggcccca
     1321 accgagttga gccgcacgcc gctccatggc ctgcacgtgc ggctcggcgc gcgcatggta
     1381 gcgttcgcag gctatgacat gccggtgcag tatccttccg gaatcatcga agagcacttg
     1441 cacacccgcg tcgccgctgg gttgttcgat gtttctcata tgggtcagat cacgctccgg
     1501 ccgcgctccg gccgtg
//
LOCUS       DBNGZE010000056         4581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1049419, whole genome shotgun sequence.
ACCESSION   DBNGZE010000056 DBNGZE010000000
VERSION     DBNGZE010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1049419"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16FV_01400"
     CDS             <1..186
                     /locus_tag="AC16FV_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR2848750.1"
                     /translation="DRARRAAHLLLGWLAVQVTLGIATLVYVVPVSLGAAHQAGALIL
                     FSLALYALHTLRVPRSV"
     gene            complement(343..3720)
                     /locus_tag="AC16FV_01405"
     CDS             complement(343..3720)
                     /locus_tag="AC16FV_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR2848751.1"
                     /translation="MAAKPDIKRRRQRRRDAALPAGRRKPVSNLLRSIATNLPALFGE
                     RYFHSLTRYLTKTLGMEYAFVCELARDRKETANVVSACFAGKILDNFSYSLVGTPCQH
                     IISHGPSVYPRQVWKLFPQDRYLADLEVECYVGVPLFGSSGQPLGLISVMGRRPLRDR
                     TTVESALQIVAARTATEIERQHLQEDLGESHRTLTTLITNLPGMVYRCRNDRDWTLEF
                     VSEGCLALTGYTADDFVGRRRITYGQLIHPDDRESVWNEVQTALKENRSFQLVYRITA
                     ADGKLKWVWEQGRGVFTSQGELLSLEGFITEITERRRAEEALRDSETRLNEAQRIAHV
                     GSWELDLAADRLTWSEEIFRIFEIDPHRFDASYEAFLDAIHPDDRAAVNRAYTESLKN
                     RTPYDITHRLRMPDGRIKYVHERCMTYYGESGAPLRSLGTVQDITERVLAELALRQSE
                     ERLRVIYDASPVVISVSRLEDGRILEVNPAFLRTGGWTREEVIGRSSFDLDLWVDPRD
                     REKIVEGVRAHGTVRDLELAFRVKSGEQRLLLCSIELIQIEPGDCLLFVGQDITERKK
                     AQVQMQKLSRALEQTADPVLITDRNGVIEYVNPAFERVAGYTSAEALGRKPSLLKSGR
                     QGPGFYENLWKTILAGEVFNDVLINRRKDGSLYYDEETITPIKDADGRITHFVATGKD
                     ITERMQTQERLAYMAQHDPLTALPNRTLLLDRLKQSLAGARWRERRAAVLFVDLDRFK
                     TINDTLGHEVGDRLLQQLAERFQRSVRDGDTVARFGGDEFVILLDDVASEDDVSGVAQ
                     KVLQALAPPFQVDGQTLYVTASIGVSLFPNDGEDAGALLKNADIAMYRAKELGKNTYQ
                     FYSADMSSRAFERLTLESKLRRALEQGDFRLYYQPQVDVTSGAIVGVEALLRWQHPEF
                     GLVMPNDFIPLLEETGLIVPTGEWVLDTACAQLAAWHAQGWPRLRLAVNLSPRQFQTQ
                     NLTAVVKQALDRLEGEPGRLELEITEGMLLRHVPATVETIEALHALGVRMAIDDFGTG
                     YSSLSYLRRLPIDTLKIDRSFVHDIPQDADDSAITVAIIALAQSLKLEMIAEGVENTA
                     QRDFLRARGCNVMQGFLFSRPLPAEEITRLLRSGKKL"
     gene            4039..>4581
                     /locus_tag="AC16FV_01410"
     CDS             4039..>4581
                     /locus_tag="AC16FV_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HIR2848752.1"
                     /translation="MSPINTPSALESSRWRVFSAAPHRMMFLPGALQTVLVMLFWLAE
                     LLGRAGWLPALSLALSSIWAHMFLMLFGLFPFFIFGFLLTVYPRWMGGPVIPVSRYVA
                     VFVFLVAGMVLFYAGLFTSRIIVALSLVLQLMGWAVALYALFAVFRQAPKRGPHERLL
                     NLALAAGAIGILCFLVGVVTQ"
BASE COUNT          799 a         1445 c         1367 g          970 t
ORIGIN      
        1 gaccgcgcgc ggcgggcggc gcacctgttg ctcggctggc tcgcagtgca agtcacgctt
       61 ggcatcgcca cgctcgtcta tgtcgtgcca gtttcgctgg gcgcggcgca tcaggccggc
      121 gcgctgattc tgttcagcct cgcgctgtac gctctgcaca ccctgcgcgt tccgcgcagc
      181 gtttagaacc accaaataaa aaagaaaaaa tggacaggat tcataggatt tttcaggatt
      241 tacaggatta agaatttcta gaatttaatc ctgttaatcc tgtttttaat cctgtaaatc
      301 ctgtgaaatt ttttctctta gaaatttcta cagaaaaccc ttctacagct tcttcccgga
      361 tcgcagcagg cgggtgattt cctccgccgg cagcgggcgg ctgaagagaa agccctgcat
      421 cacattgcat cctcgtgcac gcagaaaatc gcgttgcgcg gtgttttcca caccctcggc
      481 gatcatctcc agtttcaggc tctgcgccag cgcgatgatc gcgaccgtga tcgcgctgtc
      541 gtccgcgtcc tgcggaatat cgtgcacgaa cgagcggtcg atcttgagtg tgtcgatcgg
      601 cagacgccgc aggtatgaaa gcgacgaata gccagtgccg aagtcgtcaa tcgccatgcg
      661 cacgccgagc gcgtgcaagg cctcgattgt ctcgacggtg gcgggcacgt gccgcagcag
      721 catcccttcg gtgatttcca gctccagtct tcccggttcg ccttcgagcc tgtcgagggc
      781 ctgtttgacc actgccgtga ggttctgcgt ctgaaactgg cgcggcgaga gattgaccgc
      841 cagccgcaac cgcggccagc cttgtgcgtg ccaagcggcg agctgggcgc aggcggtgtc
      901 caggacccat tcgccggtcg gcacgatcag gccggtctcc tcgagcagcg gaatgaaatc
      961 gttcggcatg accaggccga attccgggtg ctgccagcgc aacaacgcct cgacaccgac
     1021 aatagcgccc gacgtcacgt cgacctgcgg ttgataatag agacggaaat cgccctgttc
     1081 gagcgcgcgg cgcagcttcg attcgagcgt caggcgctcg aaggcgcgcg agctcatgtc
     1141 cgccgagtag aactgataag tgttcttgcc cagctccttg gcgcggtaca tcgcgatgtc
     1201 ggcgttcttg agcagcgcgc ccgcgtcctc gccgtcgttc ggaaacaggc tcacgccgat
     1261 gctggcggtg acatagagcg tctggccatc gacctgaaac ggcggggcca gcgcctgcag
     1321 caccttctgc gccacgccgg acacgtcgtc ctcggaggcg acgtcatcga gcagaatcac
     1381 gaactcgtcg cccccgaagc gcgccacggt gtcgccgtcg cgcacgctgc gctggaaacg
     1441 ctcggcgagt tgctgcaaca ggcgatcgcc cacctcgtgg ccgagggtgt cgttgatggt
     1501 cttgaagcgg tccaagtcca cgaacagcac cgccgcgcgc cgctcgcgcc agcgcgcgcc
     1561 cgccagcgac tgcttcaggc gatcgagcag caaggtacgg tttggcagcg cggtgagcgg
     1621 gtcgtgctgc gccatatagg ccaagcgctc ctgtgtctgc atccgctccg tgatgtcctt
     1681 gccggtggcg acgaagtgcg tgatacgtcc gtcggcatcc ttgatcggcg taatggtttc
     1741 ttcatcgtaa tacagagaac cgtccttgcg gcggttgatg agcacgtcat tgaagacttc
     1801 cccggccagg atggttttcc acaaattttc gtaaaatccc gggccctgcc tgccagactt
     1861 gagcaggctg ggcttgcgcc ccagcgcctc ggcggacgta tagccggcta cccgctcgaa
     1921 cgccggattg acgtattcga tcacgccgtt gcggtcggtg atcagcacgg ggtcggcggt
     1981 ctgctcgagc gcgcgagaga gtttctgcat ctgcacctgg gctttcttgc gctcggtaat
     2041 gtcctgcccg acgaacagca ggcaatcgcc cggctcgatc tggatcagct cgatgctgca
     2101 cagcaacagg cgctgctcgc ccgacttcac gcggaaggca agttccaggt cgcgcaccgt
     2161 gccgtgcgcg cgcacgcctt cgacgatctt ttcccggtcg cggggatcga cccagagatc
     2221 gagatcgaag gaactgcggc cgatcacctc ttcccgcgtc cagccgccgg tgcgcaggaa
     2281 cgccgggttc acttccagaa tccgcccgtc ctcgagccgg gaaacgctga tgaccacggg
     2341 gctggcgtcg tagatgacgc gcagccgctc ctccgactgg cgcaacgcca gctccgcgag
     2401 cacgcgctca gtgatgtcct gcaccgtgcc cagcgaacgc aacggcgcgc ccgattcgcc
     2461 gtaataagtc atgcaacgct cgtgcacgta tttgatgcgc ccatccggca tgcgcaggcg
     2521 gtgagtgata tcgtagggcg ttcggttctt cagcgattcg gtgtaggcgc gattgacggc
     2581 ggcgcggtcg tccggatgta tcgcgtcgag aaacgcctcg taggacgcgt cgaagcgatg
     2641 cggatcgatc tcgaaaatgc ggaaaatctc ctccgaccac gtcaggcgat cagccgccag
     2701 atcgagctcc cagctgccga catgcgcgat gcgttgcgcc tcgttgagcc gcgtctcgct
     2761 gtcgcgcaac gcctcctcgg cgcgccggcg ctcggtgatc tccgtgatga agccttccag
     2821 cgagagcagc tcgccctggg aggtgaacac gccgcggcct tgttcccata cccatttcaa
     2881 tttgccgtcg gccgccgtga tgcgatagac aagctgaaac gaccggtttt ccttcagcgc
     2941 ggtctggact tcgttccaaa cggattcccg gtcatcggga tggatcagtt gtccataggt
     3001 aatcctgcgc cggccgacaa aatcgtcggc ggtatagccg gtaagggcga gacacccctc
     3061 gctcacaaac tccagtgtcc agtccctgtc attgcggcag cggtacacca tgccaggcag
     3121 gttcgttatc aatgtcgtca gggtacggtg gctttcaccc aagtcctctt gcaggtgctg
     3181 acgttcaatt tcagtggcgg tgcgcgccgc cacaatctgt aatgccgact ccactgttgt
     3241 ccgatcgcgc agcggccggc ggcccatgac cgagatcaga cccagcggct gaccggacga
     3301 tccgaacaag ggcacgccga cgtagcactc cacctccaga tccgcgaggt agcggtcttg
     3361 cggaaaaagt ttccatactt ggcggggata gacgctggga ccatgactga tgatgtgttg
     3421 acagggcgtg ccgacgagac tgtaactgaa gttgtcgagg atcttgccgg caaagcaggc
     3481 gctgacgaca ttcgccgttt ccttccggtc gcgcgccagc tcgcacacga aggcatattc
     3541 catgcccaag gttttcgtca ggtaccgcgt gagtgaatgg aaatagcgct ccccgaagag
     3601 cgccggcagg tttgtggcga tactgcgcaa gagattgctg acgggcttgc gccgtccagc
     3661 cggcaacgcc gcgtcgcgtc gtcgttgacg tctccgcttg atatcaggtt ttgccgccat
     3721 gcaccccgtc ttctgcaact tatgtaccgt tggacgccga tccggcacgg cgccatcgcg
     3781 gttttgccgt ccgcgccacc ggcgtgacga ttttccacct tgtcccctgt atttcagggg
     3841 gaaaaccgga acgcgataac ccgcgttatc tgtctttaat ttatagatca acatgcatgt
     3901 tatttccctg aaaccgggtt tttaggcgac atgcgcggcc ctcggctgaa aacgccctgt
     3961 tgcccttgat ttccatcaat gtcgccggcc gggggcgctg ctacaacgcc agcggttcac
     4021 gtcccgatga tgaccggcat gtccccgata aacactccat ccgccctgga atcgtcccgc
     4081 tggcgggtat tctccgccgc gccgcaccgc atgatgttcc ttcccggcgc gcttcagacc
     4141 gtgctggtca tgctgttctg gctcgcggaa ttgctcggac gcgccggatg gctgcccgcc
     4201 ctgtcgctgg cgctgtcctc catctgggcg catatgtttc tcatgctgtt cggcctgttc
     4261 ccgtttttca tttttggatt tttgctgacg gtttacccgc gctggatggg cggtccggtc
     4321 ataccggtct cgcgctacgt cgccgttttc gtttttctcg tcgcggggat ggttttgttt
     4381 tacgccggcc tgttcacttc acgcatcatc gtggcgctgt cactggtcct gcaactgatg
     4441 ggatgggcgg tggcgctcta cgccttgttc gccgtctttc gccaagcgcc caaacgcggg
     4501 ccgcatgaac gcctgcttaa tctcgcgctc gccgccggcg cgatcggcat cttgtgtttc
     4561 ctcgtgggtg tcgtgaccca a
//
LOCUS       DBNGZE010000057         4772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_702639, whole genome shotgun sequence.
ACCESSION   DBNGZE010000057 DBNGZE010000000
VERSION     DBNGZE010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_702639"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC16FV_01415"
     CDS             complement(<1..444)
                     /locus_tag="AC16FV_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR2848753.1"
                     /translation="MAQIFDNLPPRAALAEIARDFHARGWMAGTAGNLSMREDDHHFW
                     ITASGMPKGRLEETDFLLVRIKDGEVVERVRPDNKPSAETSIHRAIYALFPGARACLH
                     GHIVQAVIAADRAKKGVKGLRLPAIEMIKGFDIWQQNPKVDLPLFE"
     gene            619..1065
                     /locus_tag="AC16FV_01420"
     CDS             619..1065
                     /locus_tag="AC16FV_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR2848754.1"
                     /translation="MLSRQTSPRSTNADLGQKSVPPSSADGMRALVDRLWPRGLSKAS
                     ARIDLWARELAPSNELRRWYAHDAKKWPGFKRRYFAELKHRMQAVDSLSREIRKQKVT
                     LLFGSKEPRLNNAFALKQFLESRGRARQRPSRARSTAGRSRSGGQR"
     gene            1125..1376
                     /locus_tag="AC16FV_01425"
     CDS             1125..1376
                     /locus_tag="AC16FV_01425"
                     /inference="COORDINATES: protein motif:HMM:NF039800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HIR2848755.1"
                     /translation="MATGEPGLPREFTWRGRTIKVAAVRRSWRETGRCHHGSPELYVR
                     KHWYEVATGPHETMKIYFERQPRGARRGERWWLFSVSDS"
     gene            complement(1415..2311)
                     /locus_tag="AC16FV_01430"
     CDS             complement(1415..2311)
                     /locus_tag="AC16FV_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2848756.1"
                     /translation="MKREKRGGDADAGAVSSCAQCQPAGKAQINSCSRKNSTCQFCEA
                     KLYSGLSRKQVNEIRGRLSHCRSGPRQMLFRAGDSSTQLYVIREGQVKLTRTDIDGHD
                     HLLNLAGPGYFLGFDTIGNPSYSYSAETLTPTVLCRIRHSDIVRLLKKEPRVSLNVLL
                     AVNEQLAHARNLMRALGQKTAVQKVAALLLNLYPPEADGETDKAMALHLSRQEMAKIL
                     GLTVETVSRIMAALRRKGIIDAPPGRIVISARARLQALAGDRPRAKPSRRKSPARPPR
                     RVVTRTLPRRRERNNGGLLRLP"
     gene            complement(2430..4244)
                     /locus_tag="AC16FV_01435"
     CDS             complement(2430..4244)
                     /locus_tag="AC16FV_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR2848757.1"
                     /translation="MLRAIHKDKLAKGVHGEMTCVACHQDIADNKAPHKKIDAPKANC
                     VTCHEGLWKAIKKQDLAVEKARMGVVVKNIEAYKHSFHARPKSETSVKAACDDCHDTH
                     YFNVPPRGTSKRTAWHLTVPNVCGAKCHTEVLEEYVESVHGKEVMEMQNPKAAVCTDC
                     HTSHDIAKTTIDATMLLIVENCGGCHKENLVTYRDTYHGQVGKLGYTYTAKCFDCHGS
                     HGILRVDDPESTVHPNNRLKTCQQCHDGKERDEATLGFVTFSPHANDHDFERYPQMWM
                     ASKFMYGLLLFVFAYFWAHSLLWWYREYRHRKDRKSQTLVRVDELPPEETKQVRRFGL
                     IWRIAHLCFALSVMMLILTGMGLFYSYTAWAPVVMNAFGGPQVTGIIHRTSAAIMLGI
                     FFLHLIGVAVNIFRHRKTFRWFGPDSLVPNWKDVKDAWGMFVWFVGKGPRPVFDRWTY
                     WEKFDYWAVFWGMAIIGSSGMMLAFPHVTASIFPGWVFNVATLVHGEEAFLAAVFLFT
                     VHFFNNHFRPDKLPPPDIVMFTGTQSLEEFKRDHALQYQRLVDSGQLENYLVQAPSQP
                     MTLGSKILGIALITCGLILLILVTVGFFGGHAPHSFTL"
BASE COUNT          962 a         1426 c         1345 g         1039 t
ORIGIN      
        1 ctcgaacagc ggcaagtcca cttttggatt ctgctgccag atatcgaaac ccttgatcat
       61 ctcgatggcc ggcaggcgca ggcctttcac gcccttcttc gcgcgatccg cggcaatgac
      121 agcctgcacg atatggccat gcaggcaggc gcgcgcaccc gggaacagcg catagatcgc
      181 acgatgaatg gaggtctcgg ccgaaggctt gttgtccggc cgcacgcgct cgaccacctc
      241 gccgtccttt atccgcacca ataaaaaatc agtctcctcg agccggccct tgggcatgcc
      301 gctggccgtg atccagaaat gatggtcgtc ctcgcgcatg gaaaggttgc ccgccgtgcc
      361 cgccatccag ccgcgcgcgt ggaagtcgcg cgcgatttcc gccagtgccg cgcgcggcgg
      421 gagattgtcg aagatttggg ccataatcaa tcagcggctt gtgttgaagc gggatcgggc
      481 gccatcatta aagaagcctg ctccgttcgt cttgtcgata atgtagccct gctcgctgcg
      541 ctcgctcgtc ttgccatatg attagcatgt taagaggcgt tattttcaat gtccacttgc
      601 ctgttcctgc gtacgatcgt gttatcaagg cagacatcac caaggagcac gaatgccgat
      661 ctcggtcaaa agagtgtccc accatcctcg gcggacggca tgagggcgct ggtcgatcgg
      721 ctttggccgc gcggtttgtc aaaggcatcc gccagaatcg acctctgggc ccgggaactg
      781 gcaccgtcga atgaactgcg ccggtggtac gcgcacgacg cgaagaaatg gcccgggttc
      841 aaacggagat attttgccga actcaagcac cggatgcagg cggtggattc actgtcgcgc
      901 gagataagaa agcagaaagt cactctgctg ttcgggtcga aggagccgcg cctgaacaat
      961 gcatttgctc tcaagcagtt cctcgagtcg cgcggccgcg cacgccaacg cccgtcacgg
     1021 gcacgttcca ccgccgggcg ttcccggtcc ggaggacagc gatgacggag cgcttcatat
     1081 ccgaatccat ccgtcctgtg accgccacgt aggacaccgc gcgcatggcg accggggagc
     1141 cgggactgcc gcgcgagttc acctggcgcg gccggaccat caaggtggcc gccgtgcggc
     1201 gcagctggcg cgaaaccggc agatgccatc acggcagccc tgaactgtac gtgcgcaaac
     1261 actggtacga ggtcgccacc ggtccgcacg aaacgatgaa aatctatttc gagcgccagc
     1321 cacgcggcgc acgccgcggc gaacggtggt ggttgttcag cgttagcgat tcgtgatccc
     1381 ggcgccgaca gcggcgggcg ctgccggact gaagctacgg caaccgcaaa agcccaccgt
     1441 tgttccgttc acgtcgccgt ggcagcgttc gggtgacgac acgccttggt gggcgcgccg
     1501 gagatttcct cctcgaaggc ttggcgcgcg gtcgatcgcc cgccagcgcc tggagtcgcg
     1561 cgcgggcgga gataacaata cgcccgggcg gtgcatcgat aatccctttg cgccgcagcg
     1621 ccgccatgat gcggctgacc gtctccaccg tgagtcccag aatcttcgcc atctcctggc
     1681 gcgacaaatg cagtgccatg gctttgtcgg tttcgccgtc agcctccggc ggatacaggt
     1741 tcaacagcag cgctgccacc ttctggaccg ccgttttctg ccccagcgca cgcatcaggt
     1801 tgcgcgcgtg cgccaattgc tcattgaccg ccaggagcac attcagtgac acccggggtt
     1861 ctttcttgag caggcggacg atgtcgctgt gtctgatccg gcagagtacc gtcggcgtga
     1921 gcgtctccgc gctgtagctg taggaagggt taccgatggt atcgaatcca agaaaatagc
     1981 cgggacccgc caggttaagg agatgatcat ggccgtctat gtccgtgcgt gtgagtttga
     2041 cctgcccttc gcggatcacg tatagctgtg ttgatgagtc gccggcgcga aacaacatct
     2101 ggcgcggacc gctgcggcaa tgactgagac ggccgcggat ttcattgacc tgtttcctgg
     2161 agaggcccga gtacagtttc gcctcgcaga actggcaggt actatttttg cgcgaacagg
     2221 aatttatctg cgccttaccg gccggttggc attgcgcaca cgaggagaca gcgcctgcgt
     2281 ctgcgtcacc accacgcttt tcgcgtttca aagttgccct gcctcgtttc atcgtttcgt
     2341 ctctataact gcaatccaat ctgacacgaa cggccgcaag actcattgat catcgtcaac
     2401 ataaatcccc ggccgcttcg tcagcccggc tataaagtaa agctgtgtgg cgcgtggccg
     2461 ccgaagaagc cgaccgtcac cagaatcagc agtatcaggc cacaagtgat caaggcgatg
     2521 cccaatatct tcgatcccag cgtcatgggt tgagacggag cctgcaccag atagttttcc
     2581 agttgcccac tgtcgaccag gcgctggtac tgcagcgcat gatcacgctt gaactcttcc
     2641 agcgactggg tgccggtgaa catcacaata tccgggggcg gcagcttgtc cggccggaag
     2701 tgattgttga agaagtgcac cgtgaacagg aacaccgccg ccaggaacgc ctcttcgccg
     2761 tgcaccaatg tggccacgtt gaacacccaa cccgggaaga tcgacgcggt cacgtgtggg
     2821 aaggccaaca tcatgccgct gctgccgatg atggccatgc cccagaacac cgcccagtag
     2881 tcgaacttct cccaataggt ccagcggtcg aacaccggcc gcgggccctt gccgacgaac
     2941 cacacgaaca tgccccaggc gtccttgacg tccttccagt tgggtaccag cgaatcggga
     3001 ccgaaccagc ggaaggtttt acggtggcgg aaaatattta ccgcgacacc gatcagatgc
     3061 aggaagaaga tccccagcat gatcgcggcg ctggtgcgat ggataatccc cgtcacctgc
     3121 gggccgccaa acgcattcat gaccaccggc gcccaggcgg tatacgagta aaacaacccc
     3181 atccccgtca ggatcagcat catgacactc agggcaaagc aaaggtgggc gatacgccag
     3241 atgagaccga accgtcggac ctgtttggtt tcctccggcg gcagttcgtc cacgcgaacg
     3301 agcgtctggc ttttgcgatc cttgcgatgc ctgtattcgc gataccacca cagcagtgaa
     3361 tgcgcccaga agtaggcgaa cacgaacagc aagagcccgt acatgaattt cgaggccatc
     3421 cacatctggg gataacgttc gaagtcatgg tcattggcat gcgggctgaa ggtcacgaat
     3481 cccagcgtcg cctcatctct ttctttcccg tcatggcact gttggcaggt cttcagacga
     3541 ttattggggt gtacggtcga ttccggatca tccaccctca ggatgccatg actgccgtga
     3601 cagtcgaaac acttggccgt gtacgtgtag cccagtttgc cgacttggcc gtgataggtg
     3661 tcgcgatagg tcacgaggtt ctccttatgg cagcctccac aattctccac gatcaggagc
     3721 atggtggcgt caatcgtcgt cttggcgatg tcatggctgg tgtggcaatc cgtgcacacg
     3781 gccgccttcg gattttgcat ctccatgact tccttgccat gcactgattc cacatactct
     3841 tccagtacct cggtatggca cttcgcgccg caaacgtttg gcacggtcag gtgccatgcc
     3901 gtgcgtttgg aagtgccccg tggcgggaca ttgaaataat gggtatcgtg gcagtcgtcg
     3961 caggcggcct tgacgcttgt ctcgctcttg ggccgggcat ggaaggaatg cttgtacgcc
     4021 tcgatatttt ttactaccac tcccatcctt gctttttcta cggccaagtc ctgcttcttg
     4081 atggccttcc acaaaccttc gtggcaggtc acgcaattgg ccttcggtgc gtcaattttt
     4141 ttgtgcggcg ctttgttgtc ggcaatatcc tgatgacacg ccacgcatgt catctcgcca
     4201 tgcacacctt ttgcgagctt gtccttatgt atcgcccgca gcaagcgcgt ttttccgtcc
     4261 gcgccccgca cttttaattc ttgctttttt tcaccgtgac aggccaggca tgtggcatcg
     4321 tcaatttttt tggcggcttg tccaacgcca tttttcatgg gagttgcggc ggctgtcgct
     4381 tccgtctgcc aaacagcctg ggcggctgcc aaacagacag cccccgccat caaccacgtt
     4441 acaagttttg gtacttgcat ggcattggcc cctactcgaa attgcgccgc cgtttcgtcc
     4501 gatctcaaac aggacaaact tatcgtgcgg gttttcaaca gtcagggtag ataatcgttc
     4561 atcgtttaat cgtggcctcc accttcaagc ggcatgccgt tatttatcct actattcatc
     4621 gggttcgacc ctgattgata tatatcaatg tttgagttgc gacggatgtt attttggata
     4681 gcatgaaagc ggttgtggga aaactaccgt atgttgatct atatcaaagt cctcattatt
     4741 tccagcaaca tgataatcat ggatagaagt aa
//
LOCUS       DBNGZE010000058         5124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_494625, whole genome shotgun sequence.
ACCESSION   DBNGZE010000058 DBNGZE010000000
VERSION     DBNGZE010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_494625"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="AC16FV_01440"
     CDS             complement(<1..831)
                     /locus_tag="AC16FV_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HIR2848758.1"
                     /translation="MTTTRPFFATCPKGIEPLLADELRQLGAEMVKETRAGVSFEGTL
                     ATGYRVCLWSRLASRILLPLTSFPAPTPEALYAGVQTIAWKEHLAAEGTLAVDLTTSQ
                     SAITHSHFGALKVKDAIVDQLRDEFGVRPSIDTAQPDIRVNVYLLRDQATVSLDLSGE
                     SLHRRGYRTQTVQAPLKENLAAAILLRANWPAIAKQGGMLVDLMCGSGTLPIEAAMIA
                     ADIAPGLAREYFGFTRWKQHDAATWTGLLDEARLRRETGLAHLPPIHGYDHDPLAIRS
                     A"
     gene            922..1200
                     /locus_tag="AC16FV_01445"
     CDS             922..1200
                     /locus_tag="AC16FV_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848759.1"
                     /translation="MADSGYSLMARIGLDPEGKVVNEVFALIYAPGRSRDRFPENCVT
                     VMESEAAVLEGANPEKNLYPARVIGPSRSSEGFRLYYLVCWLDEEERP"
     gene            complement(1279..2133)
                     /gene="rpoH"
                     /locus_tag="AC16FV_01450"
     CDS             complement(1279..2133)
                     /gene="rpoH"
                     /locus_tag="AC16FV_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR2848760.1"
                     /translation="MTQALTLPISLYPEKGLSAYLRAVNALPVLSVSEERKLARRYRK
                     HNDLDAARQLVLSNLRYVARIARGFSGYGLPQADLIQEGNIGLMKAVKHYDPARKVRL
                     MSFAVHWIKAEIYDYILRNWRIVKVATTKAQRKLFFNLRKSRDRLGWMTRGEVDALAE
                     NLDVSPETVQQMEGRMTHADVAFDADNDADDDNFHAAPAGYLQDMRYNPEVLATQSDQ
                     EDSRHTQLQQALSTLDARSRDIIQRRWLDEDKPTLHELAAEYGISAERIRQIESKALD
                     VMKGTLEA"
     gene            complement(2287..3204)
                     /gene="ftsX"
                     /locus_tag="AC16FV_01455"
     CDS             complement(2287..3204)
                     /gene="ftsX"
                     /locus_tag="AC16FV_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR2848761.1"
                     /translation="MIKNYFLRHAQVFFYTLGQLSRAPVATFLTVAVIGITLALPAGL
                     YVAIENVQRVASGWEDNGQISLFLKQDLSEPAIEKLADKIRRLPSVSWVDYVSREAAL
                     AEFRKLSGFGEALGSLDRNPLPPVLVVHPAAKHAQPEALQSLLNDLRRYNEVELAQLD
                     LEWVRRLFAMLDLARQGVLILTAGLALAVLLIVGNTIRLAVLNRRDEIEIIKLIGGTN
                     AFIRRPFLYSGTIQGLLGGILAWLLVGLGLLLLSGPVHRLASLYGSLFTVQNMGFLAT
                     LALVGTGGLLGWLGSRLAVGRHLRAIEPH"
     gene            complement(3201..3872)
                     /gene="ftsE"
                     /locus_tag="AC16FV_01460"
     CDS             complement(3201..3872)
                     /gene="ftsE"
                     /locus_tag="AC16FV_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR2848762.1"
                     /translation="MIEFAHVFKRYPGGFDALRDINLRLEAGEMAFITGHSGAGKSTL
                     LKLITAIERPTRGEAAVNGKNLTKLPRRRIPYFRREIGVVFQDHKLLFDRTVYDNVAL
                     PLIVTGADQHEIPRRVRAALEMVGLLDKERMLPVTLSGGEQQRVGIARAVVNKPPLLL
                     ADEPTGNLDSKLSDEILDLFLDFHHHGVTVLIATHYLHLIDRGRQRVIELKHGELARD
                     TGAGS"
     gene            complement(3884..4846)
                     /gene="ftsY"
                     /locus_tag="AC16FV_01465"
     CDS             complement(3884..4846)
                     /gene="ftsY"
                     /locus_tag="AC16FV_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR2848763.1"
                     /translation="MSPSYPSGQTEKNRSTGLLGRLRDKLAATQRVFTRGLSDLLLGN
                     RALDDALFEELETLLLSADVGVETTQHLIKNITEQVARKQLNDTRAVYGALRQGMLSI
                     LEPCDRRLAITPERPFVIMVVGVNGVGKTTTIAKLANYLKNDGHTVTLAAADTFRAAA
                     IEQLKTWAGRLDIPVIAQHTGADAAAVVHDALNAAVAREADVLIVDTAGRQHTHEGLM
                     DELRKIRRVIGKTRPNAPHEVLLVLDAGTGQNALSQLKHFREAVGVTGLALTKLDGTA
                     KGGILLAMARQTGLPIRFIGVGEGMDDLREFSAEEYVDALLPTA"
     gene            4931..>5124
                     /locus_tag="AC16FV_01470"
     CDS             4931..>5124
                     /locus_tag="AC16FV_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR2848764.1"
                     /translation="MSRKFSWLLLASLWLTACGQTHETTLDNGLRVIVHEDHRSPVVV
                     SQIRYKVGSHDQPEGLTGIS"
BASE COUNT          919 a         1511 c         1682 g         1012 t
ORIGIN      
        1 cgcggaccgg atcgccagtg gatcgtggtc gtagccgtgg atcggtggca gatgcgcgag
       61 ccccgtttcg cgccgcagac gcgcttcatc gagcagcccc gtccatgtgg cggcatcatg
      121 ctgtttccag cgcgtaaagc cgaagtactc gcgcgccagt ccgggcgcga tatcggcagc
      181 gatcatcgct gcttcgattg gcagcgtgcc cgagccgcac atgaggtcca ccagcatgcc
      241 tccctgtttg gcgatggcgg gccagttggc gcgcagcagg atcgcggcgg cgaggttttc
      301 cttgagcggt gcctgcaccg tctgcgtgcg gtaaccgcgc cggtgcaggg attcgcccga
      361 gagatcgagg ctgaccgtgg cctggtcacg cagcagatag acattcaccc ggatatccgg
      421 ctgggccgtg tctatcgaag ggcgcacgcc gaattcatcg cgcagctgat caacgatcgc
      481 gtcttttacc ttgagtgcgc cgaagtgcga gtgggtgatg gccgattgcg aagtggtgag
      541 gtctacggcg agtgttccct cggcggcgag gtgttctttc caagcgatgg tttgcacgcc
      601 cgcgtacagg gcttccggcg tgggcgccgg gaaactcgtc agcggcagca gaatgcggct
      661 ggccaagcgc gaccacagac agacgcggta gccggtggcg agcgtaccct cgaacgacac
      721 cccggcgcgc gtttccttga ccatctcggc accgagctgg cgcaactcat cggccaagag
      781 cggttcaatg cccttggggc aggtggcgaa gaatgggcgt gtggtggtca tgggatgggg
      841 gcgcatgtta cgtgccccgg tgtttccggt aaatcaatat ggttgttggt cccggtaccg
      901 ggtgtgataa tccgttaatc aatggctgat tctgggtatt cgctcatggc gcgtatcggt
      961 ctcgatccag aaggcaaagt agtcaatgaa gttttcgcgc tgatctacgc gccgggccgc
     1021 agccgtgacc ggtttccgga gaattgcgtc acggtgatgg aatccgaagc cgccgtgctc
     1081 gaaggcgcca atcccgaaaa gaacctttat cccgcccggg tcatcgggcc gtcccgctct
     1141 tccgagggct tccgtctcta ctatctggtg tgctggctgg acgaggaaga aaggccgtag
     1201 ttcaatttct ggcgagatta ttccaaacaa aaagggcttc caacaggaag ccctttgttt
     1261 tgctcatcga accaaaactt acgcttcgag cgtgcccttc attacatcaa gcgccttcga
     1321 ttcgatctga cgtatgcgct cggccgagat gccgtattcc gccgccagct catgcagcgt
     1381 gggcttgtcc tcatccagcc agcgacgctg gatgatgtca cgcgagcgcg catcgagcgt
     1441 ggacagcgct tgctggagct gcgtgtggcg cgaatcttcc tggtcggact gcgtggccag
     1501 cacctcgggg ttatagcgca tgtcctgcag gtagccggcc ggcgcggcat ggaaattgtc
     1561 gtcatcggcg tcgttgtcgg catcgaacgc gacgtcggcg tgggtcatgc ggccttccat
     1621 ttgctgcacc gtttccggcg acacgtccag attttccgcc agcgcgtcga cttcgccgcg
     1681 cgtcatccag cccagccggt cgcgcgactt gcgcagattg aagaacagct tgcgctgcgc
     1741 cttggtggtc gcgaccttga cgatgcgcca gttgcgcagg atgtagtcgt agatttcggc
     1801 cttgatccag tgcacggcga aggacatcag gcgcaccttg cgcgccgggt catagtgctt
     1861 gaccgccttc atcaggccga tgttgccttc ctggatcagg tccgcctgcg gcaggccata
     1921 gccggagaag ccgcgcgcga tacgcgcgac gtaacgcaga ttggacagca ccagctggcg
     1981 ggcggcgtcg agatcattgt gtttgcggta gcgccgcgcg agcttgcgtt cctcgctcac
     2041 gctgaggacc ggcagggcat tgacggcccg caaataggcc gacaacccct tttcggggta
     2101 gagactgatg ggcaatgtca gtgcctgtgt catggcatgt gtcatgtttg ggaacctcct
     2161 gctgctcatc cttccgtctt ctcttgggta aagaatttta gcactcgaaa cctgtgagtg
     2221 ccaaacggag gaaaagttcg ttgagttacc ggttgtatgg ggcgcgaagc ggggatttca
     2281 aggatttcag tggggttcga tggcccgcag atgtcggccg accgccagtc gcgagcccag
     2341 ccagcccagc agccccccgg tgccgacaag ggccagggtc gccagaaaac ccatgttctg
     2401 gaccgtgaac aggctgccat agaggctggc aaggcggtgg accgggccgg acaggagcag
     2461 cagaccgagg ccgaccagca gccaggccag gataccgccc agcagaccct gaatcgtgcc
     2521 ggaatagagg aaggggcggc ggatgaaggc attggtcccg ccgatcagct tgatgatctc
     2581 gatttcatcg cggcggttga ggaccgcgag acggatggtg ttgccgacga tgagcagcac
     2641 cgccaacgcc aggcccgcgg tcaggatcag cacgccctgt cgggccaggt cgagcatggc
     2701 gaacagccgg cgcacccatt cgaggtcgag ttgcgccagt tcgacctcgt tgtagcggcg
     2761 caggtcgttc aatagcgatt gcagtgcctc gggttgggca tgcttggctg ccgggtgcac
     2821 caccagcacc ggcggcagcg ggttgcggtc gagcgaaccc agcgcctcac cgaaacccga
     2881 gagtttcctg aactcggcca gtgcggcctc gcgcgagacg taatccaccc acgagacgct
     2941 gggaagccgg cgtatcttgt cggcgagttt ttcgatggcc ggttcggaga ggtcctgttt
     3001 caggaacagc gagatctgac cgttgtcttc ccagccgctg gccacccgct gcacgttttc
     3061 gatggcgaca taaaggccgg ccggcagcgc cagcgtgatg ccgatcaccg cgacggtgag
     3121 gaaggtagcc accggggcgc gcgagagttg tccgagggtg taaaagaaaa cctgcgcatg
     3181 acgcaggaaa taattcttga tcacgatccg gcccccgtgt cgcgcgcgag ctcgccatgc
     3241 ttcagttcaa tgacacgctg acgcccgcgg tcgataagat gcagatagtg ggtcgcgatc
     3301 agcaccgtca cgccgtgatg atgaaaatcg agaaacaggt cgaggatttc atccgagagt
     3361 ttgctgtcga gattgccggt cggttcgtcc gcgagcaata acggcggctt gttgacgacg
     3421 gcgcgcgcga tgccgacgcg ctgctgttcg ccgccggaga gcgtcaccgg gagcatgcgc
     3481 tccttgtcga gcaggccgac catttcgagt gcggcgcgca cgcgccgcgg aatctcgtgc
     3541 tgatcggcgc cggtgacgat caggggtagg gccacgttgt cgtacacggt gcgatcgaac
     3601 agcagtttgt ggtcctggaa taccacgccg atctcgcgcc ggaaataagg gatgcgccgg
     3661 cgcgggagtt tcgtcaggtt tttgccgttg acggcggcct cgccgcgcgt cggccgttcg
     3721 atggcggtga tgagcttgag cagcgtgctt ttgcccgcgc cggaatgccc ggtgatgaag
     3781 gccatttcgc cggcttcgag ccgcaggttg atgtcgcgca gggcatcgaa gccgccggga
     3841 tagcgcttga aaacgtgggc aaactcaatc atgcggctgc atctcaggcg gttggcaaca
     3901 gcgcatcgac atactcctcc gcgctgaact cacgcaaatc gtccatgcct tcgcccacgc
     3961 cgatgaagcg gatgggcagg ccggtctggc gcgccatggc gagcaggatg ccgcccttgg
     4021 cggtgccgtc gagcttggtc aacgccaggc cggtgacccc cacggcctcg cgaaaatgct
     4081 tgagttgcga cagcgcgttc tgtccggtgc cggcgtccag taccagcaac acctcgtgcg
     4141 gggcattggg acgggttttg ccgatcacgc gccggatttt ccggagttca tccatcagcc
     4201 cttcgtgcgt gtgttgccgg cccgcggtgt cgacgatgag gacatcggct tcgcgcgcga
     4261 ccgcggcgtt gagcgcatcg tgcaccaccg ccgccgcgtc ggccccggtg tgctgcgcga
     4321 tgacggggat atcgagccgt ccggcccagg tcttgagttg ttcaatcgcc gcggcgcgga
     4381 aagtgtcggc cgccgccagc gtcaccgtgt gtccgtcatt tttgagatag ttggcgagct
     4441 tggcgatggt ggtggttttg ccgacgccgt tgactcccac gaccatgatg acaaaaggcc
     4501 tttccggcgt tatggccagc cgccggtcgc agggttcgag gatcgaaagc atgccctgcc
     4561 gcagtgcgcc gtacacggcg cgcgtatcgt ttaactgctt gcgcgcgacc tgctctgtaa
     4621 tgttctttat caggtgttgg gtggtttcca cccccacgtc ggcggacagc aacagcgttt
     4681 ccagctcctc gaacagcgcg tcgtcgagcg cgcggtttcc gagcagcagg tcggaaagcc
     4741 cgcgggtgaa gacgcgttgg gtggcggcca gcttgtcgcg cagacggccc agcaggccgg
     4801 tcgaacggtt tttttctgtc tggcctgatg ggtaagacgg actcaagaga tgatcctgaa
     4861 tggaaacttt ttcggttggg cggctctaag ctatggcaca atattataag agaataatcc
     4921 gggaaacccc atgtcgcgta aattttcgtg gctcctgctt gcgagcctgt ggctgacagc
     4981 gtgcggtcaa acgcatgaaa ccaccctcga caacggcctg cgtgtcatcg tgcacgagga
     5041 tcaccgctcc ccggtcgtgg tttcgcaaat caggtacaag gtcggcagcc atgaccagcc
     5101 cgaaggcctc accggtattt cgca
//
LOCUS       DBNGZE010000059         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1605728, whole genome shotgun sequence.
ACCESSION   DBNGZE010000059 DBNGZE010000000
VERSION     DBNGZE010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1605728"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="dapC"
                     /locus_tag="AC16FV_01475"
     CDS             <1..968
                     /gene="dapC"
                     /locus_tag="AC16FV_01475"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR2848765.1"
                     /translation="FHLNKIALAQERHVLPVAGTREALFSIAQAVIDRRVNSQRPSVV
                     MPNPFYQIYEGAALLAGARPYFLNATEDNGFRTDFADVPEEIWAKVPLVYVCSPGNPT
                     GAVLRRDDFARLIGLAHKHNFLIASDECYSEIYFDEAQPPLGLLQVAHELGIQDYKHC
                     LVFHSLSKRSSLPGLRSGFVAGDGEVMEQYHQYRTYHGCALPPPTQAASTAAWNDEAH
                     VRANRDQYRAKFDAVLKILKQVLDVDKPEAGFYLWPKLPRDDETFTRELYARENVLVL
                     PGTYLSRDFEGVNPGQNRVRMALVAPLAECAEAAQRIRNYVESLK"
     gene            1085..1906
                     /gene="dapD"
                     /locus_tag="AC16FV_01480"
     CDS             1085..1906
                     /gene="dapD"
                     /locus_tag="AC16FV_01480"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR2848766.1"
                     /translation="MSDIQSIINEAFEHRGAITPRNVDTLVKDAVHEAINLLDSGRAR
                     VAEKKNGQWVVNEWLKKAVLLYFRIEDNQFIKGGYTNYWDKVPSKFGDYNSKAFREGG
                     FRVVPPAAIRKGAYIAPSVVLMPSYVNIGGYVDEGSMVDTWATVGSCAQIGRNVHLSG
                     GVGIGGVLEPVQAAPTIIEDNCFIGARSEIVEGVIVEEGAVISMGVYIGKSTKIYNRL
                     TGEVTYGRVPKGAVVVSGNLPGADGKYSLYCAVIVKQVDEKTRSKVGINELLRDI"
     gene            1962..>2344
                     /locus_tag="AC16FV_01485"
     CDS             1962..>2344
                     /locus_tag="AC16FV_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR2848767.1"
                     /translation="MDKTLELAKELIARPSQTPRDEGCQEIMIRRLAPLGFKAERLPF
                     GDVENLWAVRGSAKPLVVFAGHTDVVPSGPRDAWKSDPYKPEVRDGHLYGRGAADMKS
                     SLAAFVTAIEDFLVKHPQHKGTIGLL"
BASE COUNT          485 a          701 c          742 g          416 t
ORIGIN      
        1 ggtttcatct gaacaagata gcactcgctc aggagcggca tgtgctgccc gtcgccggta
       61 cgcgcgaggc gctgttctcg atcgcgcagg ccgtcatcga ccggcgcgtg aattcgcagc
      121 gcccgtccgt ggtcatgccc aatccgtttt accagatcta cgaaggcgcg gcgctgctgg
      181 ccggggcgcg gccgtatttc ctgaacgcca cggaggacaa cggcttccgc accgatttcg
      241 ccgacgtgcc ggaggaaatc tgggccaagg tgccgctggt gtatgtgtgc tctcccggca
      301 acccgaccgg cgcggtgctg cggcgcgatg atttcgcgcg gctcatcggg ctggcgcaca
      361 agcacaattt cctcatcgcc tcggacgagt gctattccga aatctatttc gacgaggccc
      421 aaccgccgct ggggttattg caggtggcgc acgaactcgg cattcaggat tacaaacact
      481 gtctggtgtt ccacagcctg tcgaaacgct ccagcctgcc gggtctgcgt tctggtttcg
      541 tcgccggcga tggtgaagtc atggagcaat atcatcagta ccgcacctat catggctgcg
      601 ccctgccgcc gccgacgcag gcagcgagca cggcggcgtg gaacgacgag gcacatgtgc
      661 gcgccaaccg cgaccagtac cgcgccaagt tcgacgccgt gctgaaaatc ctgaaacagg
      721 tgctggacgt ggacaaaccc gaggccggtt tttacctgtg gccgaaactg cccagggacg
      781 acgaaacttt cacgcgcgag ctgtatgcgc gtgaaaacgt gctggtgctg ccgggaacct
      841 atctgtcgcg cgacttcgag ggcgtgaatc cgggccaaaa ccgcgtgcgc atggcgctgg
      901 tggcgccgct ggccgaatgc gccgaggccg cacagcgcat ccgaaattat gtagaatcgt
      961 tgaaataata aattgaaccg ccaagacgcc agaagtgcgc cagggatggc gcgcttacgg
     1021 agccgaggac gcaagaacgc caagatatag atagtaacgg cacttcaaac gaggaatttc
     1081 aggcatgagc gacatacagt ccatcatcaa cgaggccttt gagcaccgcg gcgcgatcac
     1141 cccgcgcaac gttgacacgc tggtcaagga cgcggtgcac gaggcgatca acctgctgga
     1201 cagcggaaga gcgcgcgtag ccgagaaaaa gaacggccag tgggtcgtga acgaatggct
     1261 gaaaaaggcg gtgttgctct atttccgcat cgaggacaac cagttcatca agggcggcta
     1321 caccaattat tgggacaagg tgccgtccaa gttcggcgac tacaactcca aggccttccg
     1381 tgagggcggt ttccgcgtgg tgccgccggc cgccatccgc aagggcgcct atatcgcgcc
     1441 ctcggtggta cttatgccct cttatgtgaa catcggcggc tacgtggacg agggcagcat
     1501 ggtggatacc tgggccacgg tcggttcctg cgcccagatt ggcagaaacg tgcacctctc
     1561 tgggggggtc ggcatcggcg gcgtgctgga gccggtgcag gccgcgccca ccatcatcga
     1621 ggacaactgc ttcatcgggg cgcgctccga gatcgtcgag ggggtgatcg tggaggaagg
     1681 cgcggtgatc tccatggggg tgtacatcgg caagagcacc aagatctaca accgcctgac
     1741 cggcgaggtg acctacggcc gggtgcccaa gggcgccgtg gtagtctcgg gcaacctgcc
     1801 cggcgccgat ggcaagtaca gcctctattg cgccgtgatc gtgaagcagg tggacgagaa
     1861 gacacgttcc aaggtcggca tcaatgagct gttaagagac atttgatcca accgaatcaa
     1921 tcttaacttt tatccgtctt tataaatagt tataagaact catggataaa acactggaac
     1981 tggcgaagga gctgattgcg cgtccgtcgc agacgccgcg cgacgaaggc tgccaggaaa
     2041 tcatgatccg gcgcctcgcg ccgctcggct tcaaggccga gcgcctgccc ttcggcgacg
     2101 tcgagaacct gtgggccgtg cgcggcagcg ccaagccgct ggtggtgttt gccgggcata
     2161 ccgacgtggt cccctccggc ccgcgcgacg cctggaagag tgacccctac aaacctgagg
     2221 tacgcgacgg ccacctgtac ggccgcggcg cggccgacat gaaatcctcg ctggcggcgt
     2281 tcgtcacggc gattgaagac ttcctggtca agcacccgca acacaagggc actatcggcc
     2341 tgct
//
LOCUS       DBNGZE010000060         2877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1537297, whole genome shotgun sequence.
ACCESSION   DBNGZE010000060 DBNGZE010000000
VERSION     DBNGZE010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1537297"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..469
                     /gene="iscU"
                     /locus_tag="AC16FV_01490"
     CDS             65..469
                     /gene="iscU"
                     /locus_tag="AC16FV_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIR2848768.1"
                     /translation="MAYSDKVLDHYENPRNVGSFDKNDAQVGTGMVGAPACGDVMKLQ
                     IKVNEQGIIEDAKFKTYGCGSAIASSSLLTEWVKGKSIEEAGKIKNTQIAEELALPPV
                     KIHCSVLAEDAIKAAIADYQTKTGKTSKADAA"
     gene            499..822
                     /gene="iscA"
                     /locus_tag="AC16FV_01495"
     CDS             499..822
                     /gene="iscA"
                     /locus_tag="AC16FV_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HIR2848769.1"
                     /translation="MAITMTPAAAERVRKFIEKRGKGEGLRLGVRTSGCSGKAYVIEY
                     ADKIEPDDLVYESHGAKVIVDPKSLIYLDGTELDYTREGLNEGFKFSNPNVKDSCGCG
                     ESFST"
     gene            843..1382
                     /gene="hscB"
                     /locus_tag="AC16FV_01500"
     CDS             843..1382
                     /gene="hscB"
                     /locus_tag="AC16FV_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HIR2848770.1"
                     /translation="MHAAPGKNHFELFGLPAAFDIDADDLASRYRELQRRVHPDKFAN
                     ASDQERRLSLQMTAQVNDAFQTLKDPVRRGRYLLSLRGVDIDEETDTAMDPAFLMEQM
                     EWRENLDEIRQADNPHKQLVELANRIEQGMQDKIGQFRQAIGKDSPEATGKARNIVRE
                     MQFLEKMRREIDNLEEELI"
     gene            1505..>2877
                     /gene="hscA"
                     /locus_tag="AC16FV_01505"
     CDS             1505..>2877
                     /gene="hscA"
                     /locus_tag="AC16FV_01505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR2848771.1"
                     /translation="MALFQISEPGQTPAPHERRYAVGIDLGTTNSLVACVRSGVAETL
                     PDEQGRHLLPSVVRYLAQGEPVVGHEAMAVAHEDPLNTIASVKRLMGRGIEDVRKLGK
                     SLPYEFSRGDSAMPRLRTVAGERSPVEISADILRSLKQRAMATLGTEIGDAVITVPAY
                     FDDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDMNPQGVYAVYDLGGGTFDISL
                     LRLNRGVFEVMATAGDSALGGDDMDHAIAEWIMRAAGIQDDAGHHQMRRLLRDARAAK
                     EALTLKDRVALRIDLDQGKNWQGELTREQLNNLIDPLIDRSLAPCKRALRDAGLRASE
                     IDGVVLVGGATRIPRVRGKVSQFFGREPLADIDPDKVVAIGAALQADVLIGNKRDEEM
                     LLLDVIPLSLGLEIMGGMVEKVIPRNTTIPVAKAQDFTTYKDGQTALLIHVVQGEREL
                     VSDCRSLA"
BASE COUNT          637 a          820 c          893 g          527 t
ORIGIN      
        1 tgcagtgggc cgcgcattaa tcaggtaaac tattattatt ttattatttc cgggggtaca
       61 aaccgtggca tacagtgaca aggtactgga tcattacgaa aacccgcgca acgtgggctc
      121 gttcgacaag aacgacgcgc aggtgggtac cggcatggtc ggcgcgcccg cctgcggcga
      181 cgtcatgaaa ctgcagatca aggtgaacga gcagggcatt atcgaggacg ccaagttcaa
      241 gacctacggc tgcggcagcg ccatcgcgtc gagctcgctc ctgaccgaat gggtcaaggg
      301 caagagcatc gaagaggcgg gcaagatcaa gaacacccaa atcgccgaag agctggcgtt
      361 gccgccggtg aagattcact gttcggtgct ggcggaagac gctatcaagg cggcgatcgc
      421 agattaccag acgaaaaccg gcaaaacctc gaaagcggac gccgcctgaa tctgaccggg
      481 ttacggagcg aaactgatat ggcaattacc atgacgccgg ccgccgcgga acgcgtgcgg
      541 aaattcatcg agaagcgcgg caagggcgag ggcctgcgcc tgggcgtgcg cacgtccggc
      601 tgctcgggga aggcgtatgt catcgagtac gccgacaaaa tcgagcctga cgatcttgtt
      661 tacgagagcc acggcgctaa ggtgatcgtg gatcccaaga gcctgattta tcttgacggc
      721 accgagctcg attacacccg cgagggcctg aacgagggtt tcaagttcag taaccccaac
      781 gtcaaggact cctgcggctg cggggaaagc ttcagtacct gaaacggcga gatgccaccg
      841 gcatgcacgc cgcaccggga aaaaatcatt tcgaactctt cggtctgccg gcggcattcg
      901 atatcgatgc cgacgacctg gcgtcgcgct accgcgaact tcagcgtcgc gttcatcccg
      961 acaagtttgc caacgcctcc gatcaggagc gccgcctgtc tctgcaaatg acagcgcagg
     1021 tcaacgacgc gttccagacg ctcaaggatc cggtgcgacg cggacgttat ctcctgagcc
     1081 tgcgtggtgt tgatatcgat gaggaaaccg acacggcgat ggacccggcg tttctgatgg
     1141 agcaaatgga gtggcgcgaa aatctcgatg aaattcggca ggcggataat ccgcacaaac
     1201 agctggtaga gctggccaac cgcatcgagc aaggcatgca ggacaagatc ggacagttcc
     1261 ggcaggctat cggcaaggat tcgccggagg caaccggcaa ggcgcgcaac atcgtgcgcg
     1321 agatgcaatt cctggagaag atgcggcgcg agatcgacaa tctcgaagaa gaactgattt
     1381 aaggaaaatc tgattaattg atttttgaac cgccaagacg ccaaggccgc caagaaagta
     1441 taagaattat ccagaaactt ggcgttcttg gcgtcttggc ggtttaataa tcgaataaat
     1501 taccatggca ctttttcaga tcagcgaacc gggacaaacc cccgcgcccc acgagcgccg
     1561 ttatgccgtg ggcatcgatc tcggcaccac caattcgctc gtcgcctgcg tgcgcagtgg
     1621 cgtggcggag acgttgccgg acgaacaggg gcggcatctg ctgccctcgg tcgtgcgtta
     1681 cctggcgcag ggagaaccag tcgtgggtca cgaggctatg gccgtggcgc atgaggaccc
     1741 gctcaacacc attgcctcgg tgaagcgtct catggggcgc ggtatcgagg atgtacgcaa
     1801 gctcggcaaa tcactgccct atgaattttc ccgtggcgat tcggcgatgc cgcgcctgcg
     1861 cactgttgcc ggtgagcgca gtccggtgga aatttccgcc gatatcctga gatcgctcaa
     1921 gcagcgcgcc atggcaacgc tgggcacgga gatcggtgac gcggtcatca ccgtgcctgc
     1981 ctattttgac gatgcccagc gccaagcgac caaggacgcc gcacgcctcg cgggcctgaa
     2041 tgtgctgcgc ctgctgaacg aacccacagc ggcggcgatt gcctacggtc tcgacatgaa
     2101 cccgcagggt gtatatgccg tctacgactt gggcggcggt acgttcgata tttccttgct
     2161 gcgcctgaac cggggcgtgt ttgaggtcat ggccacggca ggcgattccg cgcttggcgg
     2221 cgacgatatg gatcacgcca tcgccgaatg gatcatgcgc gcggccggga tccaggacga
     2281 tgccggacac catcagatgc gccgcctgtt gcgcgatgcg cgcgccgcca aggaagcgct
     2341 gacgctgaaa gatcgtgttg ccttgcgtat cgacctcgat cagggaaaaa actggcaagg
     2401 cgagctgaca cgcgagcaac tgaataacct gatcgatcct ttgatcgatc gcagtctggc
     2461 cccctgcaag cgtgcactgc gcgacgccgg cctccgtgcc agtgaaatcg acggtgtggt
     2521 actggtgggt ggcgccacgc gcatcccgcg cgtgcgcggg aaggtatcgc aattcttcgg
     2581 gcgtgagccg ctggccgaca tcgatccgga caaggtggtt gccatcggcg ccgcgctgca
     2641 ggccgacgtc ctcattggca acaagcgcga cgaggaaatg ctgctgttgg atgttatccc
     2701 cttgtccctc ggtctggaga tcatgggcgg catggttgaa aaggtcatcc cgcgcaatac
     2761 cacgatccca gttgccaagg cgcaggactt caccacttat aaagacggac agaccgcctt
     2821 gctgattcat gtggtgcaag gcgagcgcga gctggtttcc gactgccgtt cgctggc
//
LOCUS       DBNGZE010000061         2913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_633404, whole genome shotgun sequence.
ACCESSION   DBNGZE010000061 DBNGZE010000000
VERSION     DBNGZE010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_633404"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16FV_01510"
     CDS             complement(<1..310)
                     /locus_tag="AC16FV_01510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR2848772.1"
                     /translation="MTATVHVDIVSAEKEIYSGTAEMVFAPLVTGEVGILPRHAPLLA
                     RMKPGEVRVRSGNEDLSFYVSGGLLEVQPHVVTVLADTALRARDIDEAAALKAKERAE
                     E"
     gene            complement(411..1787)
                     /gene="atpD"
                     /locus_tag="AC16FV_01515"
     CDS             complement(411..1787)
                     /gene="atpD"
                     /locus_tag="AC16FV_01515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR2848773.1"
                     /translation="MANGKIVQIIGAVVDVEFPRESLPKVYDALKQPKSDLTLEVQQQ
                     LGDGVVRCIAMGTTDGLQRGLDVTNTGAPISVPVGTKTLGRIMDVLGNPVDEKGPVGE
                     EKRMPIHRQAPSYEEQAPSAELLETGIKVIDLICPFAKGGKIGLFGGAGVGKTVNMME
                     LIRNIAVEHSGYSVFAGVGERTREGNDFYHEMKEGGVIDKVALVYGQMNEPPGNRLRV
                     ALTGLTIAENFRDEGRDVLLFIDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAYEMGI
                     LQERITSTKKGSITSVQAVYVPADDLTDPSPATTFSHLDATVVLSRQVAELGIYPAVD
                     PLDSTSRQVDPHIIGEEHYRTTRDVQATLQRYKELQDIIAILGMDELSEEDKLTVSRA
                     RKIQRFLSQPFFVAEVFTGTPGKFVSLKDTIRGFRGIVDGEYDAYPEQAFYMVGSIDE
                     VAEKAKTL"
     gene            complement(1816..2682)
                     /gene="atpG"
                     /locus_tag="AC16FV_01520"
     CDS             complement(1816..2682)
                     /gene="atpG"
                     /locus_tag="AC16FV_01520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR2848774.1"
                     /translation="MAVGKEIRKQIKSIQNTQKITRAMEMVAASKMRRAQDRMQAARP
                     YAFKIRNVITHLHYAHPEYKHPFVIEREARRVGFIVITSDRGLCGGLNTNLLRTTLAT
                     MKEWKQKNAAIQICTIGGKGFGFMKRFGASIVSHTSHLGDTPRLEDLIGTVKVMLDAY
                     TKGDLDRLYLIYNHFVNTMSQKAEVEQLLPLPPSELEGDKLAHHWDYIYEPDSKEVLD
                     DLLRRYIESLVYHGVIENLACEQSARMVAMKSASDNAGGLIDELKLRYNKARQAAITQ
                     EIAEIVGGAAAV"
     gene            complement(2724..>2913)
                     /locus_tag="AC16FV_01525"
     CDS             complement(2724..>2913)
                     /locus_tag="AC16FV_01525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959383.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR2848775.1"
                     /translation="NEGFLDDVDVKKVRAFEDALQSYMKSQHGALMDSINQKPEYSDE
                     MAGQLRKVIVDFKAHHTW"
BASE COUNT          498 a          893 c          890 g          632 t
ORIGIN      
        1 cctcctcggc gcgttccttg gccttgagcg cggcagcctc gtcgatgtcc ctggcgcgca
       61 gcgcggtgtc ggccagcacc gtgaccacgt gtggctgcac ttccagcagg ccgccggaga
      121 cgtagaacga caggtcttcg ttgccggaac gcacgcgcac ctcgcccggc ttcatgcgcg
      181 ccagcagcgg cgcgtgccgc ggcaggatgc caacctcgcc ggtgacgagc ggagcgaaca
      241 ccatctccgc cgtgccggag tagatctcct tctcggcgga aacgatatcg acgtgtacgg
      301 ttgcggtcat atcaataaaa ccagtgttga gtgttaagtt gtgagtgttt agtaaaggag
      361 tgcgctccgc gcgcatcaaa tactaaacac ttagcactga gcacttatca ctatagtgtt
      421 ttcgccttct cagccacttc gtcaatggaa ccgaccatgt agaaggcctg ctccggatag
      481 gcgtcgtatt cgccgtcgac gatgccgcga aagccgcgga tagtgtcctt gagcgacacg
      541 aacttgcccg gcgtgccagt gaacacctcg gccacgaaga acggctgcga caggaagcgc
      601 tggatcttgc gcgcgcgcga taccgtcagc ttgtcttctt cggagagttc gtccatgccc
      661 aggatcgcga taatgtcctg caactcttta tatcgttgca gcgtggcttg gacgtcgcgc
      721 gtggtgcggt aatgttcctc gccgatgatg tgcgggtcca cctggcgcga ggtcgagtcg
      781 agcgggtcca cggccggata aataccaagt tccgcgacct ggcgcgacaa caccacggtc
      841 gcgtccagat gcgaaaacgt ggtcgccggc gacgggtcgg tcaggtcgtc ggcgggcacg
      901 tacaccgcct gcaccgaggt gatcgagccc tttttggtgg aggtgatgcg ctcctgcagg
      961 atgcccattt cgtaggccag cgtcggctgg taacccacgg ccgagggcat gcggcccagc
     1021 agcgcggaga cttcggtacc ggccagcgtg tagcggtaaa tgttgtcgat gaacagcagc
     1081 acgtcgcggc cttcgtcgcg gaagttctcg gcgatggtca ggccggtgag cgccacgcgc
     1141 aggcggttgc ccggcggctc gttcatctgg ccatacacga gcgccacctt gtcgatgacg
     1201 ccgccttcct tcatttcgtg atagaagtcg ttgccttcac gggtacgctc gccgacgccc
     1261 gcgaacacgg aataacccga gtgctcgacg gcgatgttgc ggatcagctc catcatgttc
     1321 acggtcttgc ccacgccggc gccgccaaac aggccaatct tgccgccctt ggcgaatggg
     1381 cagatgaggt cgatgacctt gatgccggtc tccaacagct cggcgctcgg tgcctgttct
     1441 tcataggaag gcgcttggcg gtgaatcggc atacgttttt cctcgccgac ggggcctttc
     1501 tcgtccaccg gattgccgag cacgtccatg atgcggccca aggtcttggt gccgaccggc
     1561 accgagatcg gcgcgccggt attggtcacg tcgagcccgc gttgcaggcc gtcggtcgtg
     1621 cccatggcga tgcagcgcac cacgccgtcg ccgagctgct gctgcacctc cagcgtcagg
     1681 tcggacttgg gctgtttcag ggcgtcatag accttcggga gcgattcgcg cggaaattcc
     1741 acgtccacga ccgcgccgat gatttgtacg atttttccgt tagccatctt tcaatgcctc
     1801 aaacttgtga attcgttata ccgccgcggc gccgccgacg atctcggcga tctcctgcgt
     1861 gatcgccgcc tggcgcgcct tgttgtagcg cagtttcaat tcgtcgatca ggccgccggc
     1921 attgtcgctg gcggatttca tcgccaccat gcgcgccgac tgctcgcacg cgagattctc
     1981 gatcacgccg tgatacacca gcgattcgat gtagcgccgc aacaggtcgt cgagcacttc
     2041 cttcgaatcg ggttcgtaaa tgtagtccca gtgatgcgcg agcttgtcgc cttccagctc
     2101 cgagggtggc agcggcagca gctgttccac ctcggctttc tgcgacatgg tgttcacgaa
     2161 atggttgtag atcaggtaca gccggtcgag atcgcccttg gtataggcgt cgagcatgac
     2221 cttgaccgtg ccgatcaggt cctccagccg cggcgtgtcg ccgaggtgcg aggtgtgcga
     2281 aacgatgctg gcgccgaaac gcttcatgaa gccgaaaccc ttgccgccga tggtgcagat
     2341 ctggatcgcg gcgtttttct gcttccactc cttcatggtc gcgagcgtgg tgcgcagcag
     2401 gttggtgttg agcccgccgc acagtccgcg gtccgaggtg atgacgatga aaccgacgcg
     2461 cctggcctcg cgctcgatca cgaacgggtg cttgtactcg ggatgcgcgt agtgcaggtg
     2521 cgtgatgacg ttgcggatct tgaatgcata cgggcgcgcc gcctgcatgc ggtcttgggc
     2581 gcggcgcatt ttgctggccg cgaccatctc catggcacgc gtaatcttct gcgtgttctg
     2641 gatcgacttg atctgcttgc gtatctcttt gccgacggcc attaatgtaa ctcgctcgtc
     2701 tcgttaatga actttaagaa tttttaccag gtgtgatgcg ccttgaaatc gacgatgacc
     2761 ttgcgcagct gtcccgccat ctcgtccgag tactccggct tctggttgat gctgtccatc
     2821 agcgcgccgt gctgcgattt catgtacgac tgcagcgcgt cctcgaaggc acgcaccttc
     2881 ttcacgtcca cgtcgtcgag gaaaccctcg ttc
//
LOCUS       DBNGZE010000062         3634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1328869, whole genome shotgun sequence.
ACCESSION   DBNGZE010000062 DBNGZE010000000
VERSION     DBNGZE010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1328869"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC16FV_01530"
     CDS             <1..425
                     /locus_tag="AC16FV_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848776.1"
                     /translation="QSVELVPPVTTAPGTGTIGYWKNHPSEWPASCNLVLPGSDSNPA
                     VFAMSVLEAPVKGDKTVSLSKQLIAAKLNVCAGNATTCTIDSVSYDINQTITSAMTWL
                     TNYGPVFNKQKSWFGGGDVYQEILDDYNNGKLCAPHRP"
     gene            680..2743
                     /locus_tag="AC16FV_01535"
     CDS             680..2743
                     /locus_tag="AC16FV_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2848777.1"
                     /translation="MFQATVFCCLTGILSVGAAMFAVAAPVSGGAGYSHAIQQRMDQA
                     RQSPAGAAAVVTVIVESDSSGEIATRVAAQGGTLRYRWNRLHEVSIPTGKLSSLIRAL
                     PEAAVVRMPYPHEPAAVTGQGVALTGAGDMQALGQAGAGVTIGVIDLQFSNYTNSQAS
                     GDLPGSLVITDYTGTGTGGGTHGTNVAEIVHEMAPGAALRLAKIGSETQLSQAVDDMI
                     AAGTDVIVHSAAWFNVAFYDGTGLLCDIANRAETAGVQWVNAAGNYRTAHYLGTFTDT
                     NADLRHEFAANQNYNTIYLTAGAQVSIYLNWNAYPTTDVDYDLYLYNGNPDAGGTVVG
                     SSTNSQSGKGASRYPYPDEDISAFVPATTGTYYIVVRKKTSSVANLRFSLFSTGPSLG
                     VITTASSITQPADCLNVVGVGATNLSDVPEGYSSEGPTTDGRAKPEVSGPDGVKTSLT
                     SAFYGTSGSTPHVGAAVALLRAQNPGLTLPQIRWLLTSTAKDVNTSGFDYRTGNGRMS
                     LDADGDGYNHDTDNCPLVSNVNQLDTDGDKLGNACDADDDNDGLSDIFESSIGTNPLV
                     TDTDGDGKSDYAEVCYDGDCTKYTPGKDLSPLSMDTDGDSIPDGSDPAPLVFNPAGDI
                     APLGIPDGVVDAADYSIALRIVLGQLPATSLELSRGDLYPAGAPDGVIDLSDMALILQ
                     KVRQY"
     gene            2804..>3634
                     /locus_tag="AC16FV_01540"
     CDS             2804..>3634
                     /locus_tag="AC16FV_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848778.1"
                     /translation="MLKPHTRFLIITFCISLLGACAGARTFHDLARAGDTVAVAAGRM
                     KNFSRNNITVTITPASGSPIVYAPNDPAIRAVINLYPDPVSSLSVSMETGQDLTPSAT
                     TYGSFMLNYIPQREKDWWQTSVFIDLPASLPVGTASIEISDTSGGYAYSELEIVAGAG
                     RPNPFYASVNGPLSRNQLATMERVPHFTVDFNGSAIPFAIQIDLSHGPDVDHGGSGRA
                     HVVNPRGDLKNVTWSDTGTSMRVVILPAKAQALSNIHDYKFYVAGGVTNLTVLNIKAV
                     D"
BASE COUNT          806 a          976 c         1044 g          808 t
ORIGIN      
        1 gccaaagcgt tgaattggtg ccgccggtca cgacagcacc gggcacgggt acgatcggtt
       61 actggaagaa ccacccgagc gaatggcccg cgagctgcaa cctggtgctg ccgggttccg
      121 acagcaatcc cgccgtgttc gccatgagcg ttctggaggc gccggtcaag ggcgacaaaa
      181 ccgtatcgct gagcaagcaa ttgattgccg ccaagctcaa tgtgtgcgcg ggtaacgcca
      241 ccacatgtac catcgacagc gtgagctacg atatcaatca aaccatcact agtgcgatga
      301 cttggctgac caactacggg ccggtcttca acaaacagaa gagctggttc ggcggcggtg
      361 acgtttacca ggaaatactc gacgactaca acaacggcaa actctgcgct ccgcatcgtc
      421 cttgattgcc aaggcaatga gtgccaacag gtaaaaagga agctctatat ctgaaaatta
      481 aagtacagga acagagggta atggctggct aaacggggcg ctgttgattc agcgccccgt
      541 ttttttctga tcatgaaaat tgtttatgat gtaacatggt tttggtgtgt tagcgggccc
      601 tgattcaatt ttcacattta cgagggtctg tgggtggagg agaggcagat tggcgttact
      661 tagcatcagg ctccgtttta tgttccaagc cacggtgttc tgctgcctga ctggtatttt
      721 gtcggtcggc gcggcgatgt tcgccgtggc ggctcccgtg agcggcggag ccggatattc
      781 gcatgccatc cagcaacgca tggatcaggc tcgccagagc ccggccggcg cggctgcagt
      841 agtgacggtg atcgtcgaat cggactcctc cggcgaaatc gcgacgcgtg tagccgcgca
      901 gggcggcacg ctgcgttatc gctggaaccg tttgcacgaa gtttccattc ccaccggcaa
      961 gctgtcttcc ctgattcgtg ccttgccaga ggcagcggtg gtgcgcatgc cgtatccgca
     1021 cgaacccgcc gccgtgaccg ggcagggtgt tgcgcttacc ggcgcggggg acatgcaggc
     1081 tcttggacag gcgggcgccg gagtgacaat tggtgtcatt gacctccagt tctccaacta
     1141 caccaattcc caggcctccg gcgacctgcc gggcagtctc gtcatcacgg actacaccgg
     1201 caccggcacc ggtggtggaa cgcacggcac caatgtcgcg gagatcgttc atgaaatggc
     1261 gccaggggcg gcattgcgcc tggccaagat aggatccgag acccagcttt cgcaggccgt
     1321 ggatgacatg attgccgccg ggacggacgt catcgttcat tcggcggcat ggttcaacgt
     1381 tgctttttac gatggaaccg gtcttctgtg tgatatcgcc aaccgggccg agaccgcggg
     1441 cgttcagtgg gtcaatgccg caggaaatta tcgaactgcg cattacttgg gcacgttcac
     1501 ggacaccaat gctgatctgc gacatgagtt tgccgctaac cagaattaca acaccatcta
     1561 ccttaccgcc ggggcgcaag tttcgatcta cctaaactgg aatgcatatc cgacgacgga
     1621 tgttgattat gacctgtatt tgtacaatgg caatccggat gcggggggaa ccgtagtggg
     1681 ctcctcgaca aacagccaat caggcaaggg cgcgagtcgt tacccgtatc ctgacgagga
     1741 catctcggca tttgtaccag ctactaccgg cacttattat atcgttgtcc gcaagaagac
     1801 cagttcggtc gccaatctgc gtttttcgct gttttcgaca ggcccctctt tgggagtgat
     1861 aaccaccgcc agcagtatta cgcagccggc ggattgtctg aatgtggtgg gcgtgggcgc
     1921 gacgaatctc agcgacgttc ccgagggtta cagttcggaa ggcccaacaa cagatggccg
     1981 ggccaagcca gaagtgtccg gaccggatgg tgtgaaaacg tcgctgactt cggcgtttta
     2041 cggaacttct ggctccacgc cgcacgtggg cgctgccgtg gcattgctgc gggcacagaa
     2101 cccgggtctt acgcttccgc agataagatg gctcctgacc agtacggcca aggatgtcaa
     2161 tacgtctggt ttcgattatc gcacaggcaa tgggcgcatg agcctggatg ctgatggtga
     2221 tggctataac catgatactg acaactgtcc gttggtatcc aacgtcaatc agcttgacac
     2281 cgatggcgac aagctcggaa atgcctgtga cgctgatgat gataatgatg gtctgtcaga
     2341 catattcgaa tcatcgatcg ggaccaaccc attagtgacg gacacagatg gcgatggcaa
     2401 gagcgattat gctgaagtgt gttatgacgg agattgcacc aaatacaccc cgggaaaaga
     2461 cctcagcccg ttatcgatgg acacggatgg tgacagcatt ccggatggca gcgatccagc
     2521 acccctcgtt ttcaatcctg ctggtgatat agcgccgttg ggaattccag acggcgtggt
     2581 cgatgccgcg gattattcca ttgcgctgag gattgttctg ggacagcttc cggccacgag
     2641 cctcgagttg tcgcgcggcg atctttatcc cgctggggcg ccggatggag tgatcgatct
     2701 ctcggacatg gcgctgattc tgcaaaaagt tcgacagtat tgattaattg ttttaagacg
     2761 gattcatggt cgaaagaaaa tagatttgaa cggagtgtta gtcatgctaa aaccacacac
     2821 caggttcctc atcatcacat tttgtatatc actgcttgga gcatgcgccg gcgccaggac
     2881 ctttcatgac cttgcccgcg ccggggacac cgtggcggtg gcggcagggc gcatgaagaa
     2941 cttctcgaga aataacatta ccgtcaccat cacgcccgcc agtggcagcc cgatcgtcta
     3001 tgcgccgaat gatcccgcca taagagccgt tatcaacctc tatccggatc ccgtttccag
     3061 tctgtctgtt tcgatggaaa ctggtcagga tctgacgccg tctgcgacta cgtacggaag
     3121 tttcatgctt aactacatcc cccagcgtga aaaagattgg tggcagacat cggtttttat
     3181 cgacctgccg gcgagtctgc cggtaggaac cgcctctatc gaaatttctg acacctccgg
     3241 tggatacgcg tattcggagc tggaaatcgt ggcgggtgcg ggcaggccca atccatttta
     3301 tgcgagtgtt aacggaccgc ttagtcgcaa tcagcttgcc acgatggaac gcgtcccgca
     3361 ctttaccgta gattttaatg gaagtgcgat cccattcgct attcagattg acttgtctca
     3421 tggtcccgac gtggatcatg gaggatcggg ccgggcccat gtggtaaatc cccgcggcga
     3481 tctgaaaaac gtgacttggt cggatacggg aactagcatg cgagtcgtta ttctgccagc
     3541 caaagcgcag gcattaagca atatccacga ttacaaattc tacgtggccg gcggtgtaac
     3601 caaccttacc gtgctgaaca tcaaagccgt ggat
//
LOCUS       DBNGZE010000063         3752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_703653, whole genome shotgun sequence.
ACCESSION   DBNGZE010000063 DBNGZE010000000
VERSION     DBNGZE010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_703653"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..964)
                     /locus_tag="AC16FV_01545"
     CDS             complement(152..964)
                     /locus_tag="AC16FV_01545"
                     /inference="COORDINATES: protein motif:HMM:NF037870.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein P2"
                     /protein_id="HIR2848779.1"
                     /translation="MRITRKLTNIQNALAGKTCQIICPVKFTYDEIILAYSGTAVTRA
                     MLKNITVEANGEEIQKFKDGDRLDQYNDYYGRSDTAGYLTLWFNRREMKTLQDQRITG
                     LGTADLNTLVVKIDIDATAPADFALLATAKLSDPQPLGLFTRIKTVNYNSAVAGQVDV
                     DKIPVGPRVLAIHQFKADITNTEVYLDEVKVYEALKTVAEVDQKNYGRTPQTAVATHI
                     DFGLEGDIAQAMVTAAPQANRNTMDLLVKPTLGTSGAVDFLVEYLDALKKAA"
     gene            complement(1247..1891)
                     /locus_tag="AC16FV_01550"
     CDS             complement(1247..1891)
                     /locus_tag="AC16FV_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848780.1"
                     /translation="MQPRSIDVTLGSRITAVVGMSRSGKSAYVKRQVKTRRRLLVWDP
                     DGEFVDAGICQPLTARQQLLDVVSRQAGGRYSYIAPVRRDEFNFWSLAVLHWRNCAAV
                     AEETSDVTSPGKAPEQWGQLIRKGNKRGIELYAVTQAPSESDKTVMRQARFVVCFMLG
                     RDVDRAYMAAELDLPKERLQQLRTVEREFAEGLVVDKHCFNGFRVKPLRLTFKK"
     gene            complement(1872..2375)
                     /locus_tag="AC16FV_01555"
     CDS             complement(1872..2375)
                     /locus_tag="AC16FV_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848781.1"
                     /translation="MSDPDQTVDQGTPAGDLDPTAAQERLQLEVLAAETAAPAAGADL
                     ETEQPAAPVINPETTARYKKILVASLKPLTSRWTRCEPLNDDEIDGLSEAWGQYLAYK
                     FPDSGPATPLGQALAVSLMVLYPRAMQLLESRKKQTAPDALVAPEKKTGSTMLDDLKD
                     FDAAPKH"
     gene            complement(2368..2769)
                     /locus_tag="AC16FV_01560"
     CDS             complement(2368..2769)
                     /locus_tag="AC16FV_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848782.1"
                     /translation="MAREDIGEVTCPFCGSVAKVRKSSPKKGEGQGKLYYSCPNDGLV
                     MPLMPDFQNWMLEHATMWGADRAERQAAAAIEKTNAPSPTAASAAAPAPAASVAGDKE
                     SGKPAAAPAVKARKKKIIGVGMYRQEVEVDE"
     gene            complement(2759..2989)
                     /locus_tag="AC16FV_01565"
     CDS             complement(2759..2989)
                     /locus_tag="AC16FV_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848783.1"
                     /translation="MIEKLNRLKKRLAQVKAGPAAIALKSAGLGDVLTDVEGLVEDLV
                     NQVIDQRDKLELLANVVDELVMEQGAGVVDGA"
     gene            complement(2986..>3752)
                     /locus_tag="AC16FV_01570"
     CDS             complement(2986..>3752)
                     /locus_tag="AC16FV_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848784.1"
                     /translation="IKTLEVTHGDNGFHPHTHSIWISEKQLTKGEAEQLEQQLFDLWL
                     AACLKRGLPAPSREHGVDVRIARHDIAEYVSKWGFAAELSGTVSKRGKNGSRNAWQLL
                     LDADQDDSNAARLWQIFAQAFFGKRQLFWSKRKTGKKITRPIYKKGPLTGKRVKVGER
                     VADEWISLREELGLAPELTEQELLDLDDADNREHQVLVLDLDTWFVVRKAGAQEAILH
                     HAVHDIRGLYALLNHLRATVPMEDGRPPGIKLEWEL"
BASE COUNT          633 a         1140 c         1174 g          805 t
ORIGIN      
        1 ctgcgcgtcg acgatgcggt tgctgacctc ggcgctgttc ttctcacggt agccgctgac
       61 agcggcatcc aggagatccc gggaaccgcc gagcaacacg tcctgcaatg tttcacctgc
      121 cataaaaaga cctccgttgc agacgcggcg attaggcggc cttcttcagc gcgtcgaggt
      181 actccacgag aaaatccacc gcgccgctgg tgccgagcgt gggtttgacc agcaggtcca
      241 tggtgttgcg attggcctgc ggcgcggccg tgaccatcgc ctgggcgatg tcgccttcga
      301 gtccgaagtc gatgtgcgtg gcgacggcgg tctgcggcgt gcggccgtag ttcttctggt
      361 cgacctcggc gacggtcttg agcgcctcgt atactttcac ctcgtcgaga taaacctcgg
      421 tgttggtgat gtcggccttg aactggtgga tcgccagcac gcgcggcccg accgggatct
      481 tgtccacgtc cacctggccc gccacggcgc tgttgtaatt gacggtcttg atgcgggtaa
      541 acagaccgag cggctgcgga tcggagagct tggcggtcgc gaggagtgcg aagtcggccg
      601 gcgcggtggc gtcgatgtca atcttgacga ccagagtgtt gaggtcggcg gtgccgaggc
      661 cggtgatgcg ctgatcctga agcgttttca tctcgcgccg attgaaccag agcgtgagat
      721 agccggcggt atcgctgcgg ccgtaataat cgttgtactg gtcgaggcgg tctccatcct
      781 tgaacttctg gatctcctcg ccattggcct cgacggtgat gttcttcagc atggcgcggg
      841 tgacggcggt gccgctatag gccaggatga tttcatcgta ggtgaatttg accgggcaga
      901 tgatctggca agtcttgcca gcgagggcgt tctggatgtt ggtcagcttg cgcgtgatac
      961 gcatttaaaa acctccggtg aggaccacac cgcctcccac cggtgccgtc gtgctggtta
     1021 agtttgttcg gcgatcagag cgggtgtgtg atcttcttcg tcatcggcca ctgggactca
     1081 gccacgttga ccaggatgat catcagcacc gcgatgccgg cagtctgcaa aaaagctttt
     1141 gtcgctgcat tcatgttgag acgctccaag gttagagaga gatacgcgac gaaataaata
     1201 cgaggattta attaggcgca aggcgctgcc taattataag gcagggctat tttttgaagg
     1261 tgagacgcag cggcttgacg cggaatccgt tgaaacaatg cttgtccacc accaggccct
     1321 cggcgaactc gcgctcgacg gtgcgcagct gctggaggcg ttctttcgga agatccaact
     1381 cggcggccat gtaagcgcgg tcgacgtcgc ggccgagcat gaagcacacc acgaaccgcg
     1441 cctggcgcat cacggtctta tcgctctcgc tcggagcctg ggtgacggcg tagagctcga
     1501 ttccgcgctt gttgcccttg cggatcagct gaccccattg ttccggcgcc ttgcctggtg
     1561 atgtgacgtc ggaggtttcc tcggccacgg cggcgcaatt gcgccagtgc agcaccgcga
     1621 gcgaccagaa attgaactcg tcgcggcgca ccggcgcgat gtagctatag cggccgccgg
     1681 cctggcgact cacgacatcg agcagctgct ggcgcgcggt gagcggctga cagatcccgg
     1741 cgtcaacgaa ctcgccatcg ggatcccaca ccaggagccg gcggcgtgtc ttcacctggc
     1801 gtttgacgta ggcgctcttg ccggagcgcg acatgccgac tacggcggtg atgcggctgc
     1861 ccagggtgac gtcaatgctt cggggctgca tcgaagtcct tgaggtcgtc gagcatggtg
     1921 gatccggttt ttttctctgg cgcgacgagc gcgtctggcg cggtctgttt cttgcgcgac
     1981 tccaggagct gcatggcgcg cggatagagc accatcaacg aaaccgcgag cgcctgaccg
     2041 agcggcgtgg ccggtccgga gtcggggaat ttataagcga ggtattggcc ccatgcctcc
     2101 gacagtccgt cgatttcatc atcgtttaag ggctcgcagc gggtccagcg cgaggtgagc
     2161 ggctttaaag aggcgacgag gattttttta taacgcgcgg tcgtttcggg gttgatgacc
     2221 ggcgcggccg gctgctcggt ttcgagatcc gcaccagccg cgggcgcggc ggtctcggcc
     2281 gcgagcacct ccagctggag ccgctcctga gcggcggtcg gatcgagatc gcccgcgggc
     2341 gttccctggt cgacggtctg gtcgggatca ctcatcgact tcgacctcct ggcggtacat
     2401 gccgacgccg atgatttttt tcttcctggc cttcacagct ggagcggcag ccggcttgcc
     2461 gctttccttg tcgcccgcaa cagacgccgc cggcgcgggg gccgccgcac tggcggcggt
     2521 cgggctgggt gcattcgttt tttcgatcgc cgcggctgcc tgacgctcgg cgcgatccgc
     2581 gccccacatg gtcgcgtgct ccagcatcca gttttgaaag tccggcatga gcggcatgac
     2641 caggccatca ttggggcagg agtaatacaa cttcccctgg ccctcgcctt ttttcgggga
     2701 gctcttgcgg accttggcga cggatccaca gaacgggcag gtgacttcgc cgatatcttc
     2761 acgcgccatc taccaccccc gcgccctgct ccatcacgag ctcatcgacg acgttcgcca
     2821 acaactccag tttgtctctc tggtcgatga cctggttcac cagatcctcg accaggccct
     2881 cgacgtcggt tagcacgtcg cccaggccgg cggacttcaa tgcgatcgcg gccggacccg
     2941 ccttcacttg cgcgaggcgc tttttcagtc gattcaattt ttcgatcata attcccactc
     3001 cagttttatc cccggaggtc ggccgtcttc cattggcacg gtcgcccgga gatggtttaa
     3061 caacgcatac agccctcgaa tgtcatgtac tgcatgatgc agtatcgctt cctgcgcccc
     3121 ggccttacgc accacgaacc aggtatcgag atcgagcacg agcacctggt gctcgcggtt
     3181 gtcggcatcg tcgaggtcta gcaattcctg ctcggtcaat tccggcgcca gtcccaattc
     3241 ttcccgcagc gagatccact cgtccgcgac gcgctcgccg accttcacgc gcttgccggt
     3301 gagcgggcct tttttataaa tcggccgggt tatttttttc ccggtcttgc gcttcgacca
     3361 gaacaattga cgtttaccga aaaaagcctg ggcaaaaatc tgccagaggc gcgcggcgtt
     3421 cgagtcgtcc tggtcggcgt cgagtagcag ttgccaggca ttgcggctgc cgtttttacc
     3481 gcgcttcgag acggttccgg aaagctcggc ggcgaagccc cacttcgaga cgtactcagc
     3541 gatgtcgtgg cgcgcgatcc gcacatcgac gccatgctcg cgcgacggcg ccggcaggcc
     3601 gcgcttgaga caggccgcga gccagagatc gaacagctgc tgctccagct gctcagcctc
     3661 tcctttcgtc agctgctttt ccgagatcca gatggagtgc gtgtgcgggt gaaacccgtt
     3721 atcgccgtgc gtgacctcta gggtcttgat ct
//
LOCUS       DBNGZE010000064         9394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_148194, whole genome shotgun sequence.
ACCESSION   DBNGZE010000064 DBNGZE010000000
VERSION     DBNGZE010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_148194"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..437)
                     /locus_tag="AC16FV_01575"
     CDS             complement(60..437)
                     /locus_tag="AC16FV_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR2848785.1"
                     /translation="MDDNSITGEIIGAAIEVHRHLGPGLLESVYRDCLWHELGLRGLA
                     VEREVAFPVRYKETEISSGYRVDLLVQRSVIVELKAVETLLPIFTSQLLSYLRMSGLK
                     LGLLINFNVAQLKDGVRRVVNNF"
     gene            complement(477..1955)
                     /gene="trpE"
                     /locus_tag="AC16FV_01580"
     CDS             complement(477..1955)
                     /gene="trpE"
                     /locus_tag="AC16FV_01580"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR2848786.1"
                     /translation="MSLSKKEFDNHAAQGFNRIPLMREVLADLDTPLSIYLKLARGPY
                     SYLFESVQGGEKWGRYSIIGLPARTLLRISGNEVTVEKDGKVIQRQTVTDPLETVRRF
                     RAQYTVPEIHGLPRFTGGLVGYFGYETIRYVEPRLGQMTKPDAIGAPDILLMVSDEVV
                     VFDNLQGRLYVVIHVNPAIADAYAKANARLDELVTGLSKSIPRAVAPESNKAVAEADF
                     ISGFTQQGFEKAVERSLEYIRAGDIMQVVLSQRLAIPFAAAPLDLYRALRTLNPSPYM
                     YFMDLKDFHIVGSSPEILVRLEDGLVTVRPIAGTRPRGATEPEDQALEKELLADPKER
                     AEHIMLVDLGRNDVGRVAKVGSVRVSDKMVIERYSHVMHIVSNVTAELKPGLDAIDVL
                     RATFPAGTVSGAPKVRAMEIIEELEPTRRGIYSGAVGYIGWNGNMDTAIAIRTAVIKD
                     KTLYIQAGAGIVADSVPRNEWNETMNKARAIFRAVAMVSKGL"
     gene            complement(2068..2778)
                     /locus_tag="AC16FV_01585"
     CDS             complement(2068..2778)
                     /locus_tag="AC16FV_01585"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR2848787.1"
                     /translation="MTPTTARKIASFPLAVKMVMIDLDGTLIDTVPDLAVAANRMLAD
                     LGRPAWGIEHYRAWIGNGVPRFVKRALTGEMQAEPEAELNERALALFRRHYGEAVSDL
                     SRPYPGVVEALEQLTAQGYSLAGLTNKAEAFTLPLLKNLELHKYFKLVLSGDSLPKQK
                     PDPLPLQHACRHFGIDTSHGVLVGDSSNDVHAARSAGMPVICVTYGYNHGHDIRQSLP
                     DAVVDSLTEVPQYLRLYT"
     gene            complement(2775..3467)
                     /gene="rpe"
                     /locus_tag="AC16FV_01590"
     CDS             complement(2775..3467)
                     /gene="rpe"
                     /locus_tag="AC16FV_01590"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR2848788.1"
                     /translation="MRDYKIAPSILSADFARLGEEVTNVLASGADIVHFDVMDNHYVP
                     NLTIGPLVCEALRKHGVKADIDVHLMVKPVDRIIPDFAKAGATYITFHPEGSEHIDRS
                     LQLVRDSGCKSGLVFNPATPLDYLKYVMDKVDMILLMSVNPGFGGQSFIKSSLDKLRE
                     ARKMIDASGRQIRLEIDGGVKVDNIREIAEAGADTFVAGSAIFNAGKAGDPNRYNSVV
                     KAMRDELAKAKA"
     gene            complement(3693..4445)
                     /locus_tag="AC16FV_01595"
     CDS             complement(3693..4445)
                     /locus_tag="AC16FV_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR2848789.1"
                     /translation="MRLTIYGSRPTRPICYHSRMPALENLGLEDLRDHAVKTFRQGGG
                     SRPDVLLIRFGSEEAVLKDYGRADPWFRRMVGPLSARREARALEQLKGARGVPRLLRR
                     VGPDALLVEYIPGTSAREVPHGGLTPQFFERFYRLVDRLHEYGVAHCDLRSQGNILVG
                     ADGEPHVVDFAAHFRRGRSWNPASRWMYAKFCEADRVAVARLKKSHAPELLTETEMQA
                     LARDRKTWLERGARIIGKSVRNISRWLLTKRA"
     gene            4501..4959
                     /locus_tag="AC16FV_01600"
     CDS             4501..4959
                     /locus_tag="AC16FV_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR2848790.1"
                     /translation="MPVAITLHILSAVVWVGGMFFAWMALRPVAASLLEPPLRLPLWS
                     QTFVRFFPWVWAAVVLLPASGYWMIFEVFKGMHNAGLYIHVMQVIGIIMITIFLHLFF
                     VPYRRMNKAIAASDFPAAGKHLAAIRKLIGINLVLGLITVTVASGGAYLG"
     gene            4964..5440
                     /locus_tag="AC16FV_01605"
     CDS             4964..5440
                     /locus_tag="AC16FV_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2848791.1"
                     /translation="MKKLVLPFVSFAWLAFLHGCAPLRSNPDMIPVESDPSGAEVYVM
                     GKKVGITPLEVRQIDVFPLTYSPEQQDLYGKIVLKKEGCTEHRQPVSTKVYAKGAKIK
                     LDCGGAAPEAAKATRPAAPETSSVENRLRQLKDLREKGLITEEEEKETRQRILKDL"
     gene            complement(5540..6796)
                     /locus_tag="AC16FV_01610"
     CDS             complement(5540..6796)
                     /locus_tag="AC16FV_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848792.1"
                     /translation="MKTLKYVVSAVLLTWSFVWMQAAQANPGFARQTGMTCNACHFQH
                     YPAVNAFGRAFKQEAYTFKGKQETIEDENLSLPVDLNIGLVTKLRYVKTNGEVKTSAT
                     NTGDIQFPDEAALLIGGRAGEHIGFLVEASLIDTASNYNSFRIHFNKKAEGVNYGIVP
                     FLTDAGGAPYGMELLNTGAQRFIRIAENRTATAAQQFVGSSGGNRPGGSEATGFAFVA
                     SAPQWYANYTLWHPTFGTSIAHEKFANYFRGAYMPNIGGWDTAFGIQLFGGTSEIMNT
                     TTSTVDKTDTKASFVDAQTQGTMGGKPVGLYFTYGKADATPAGGTTNFFNSRANDQKA
                     WSLLGEAGVIPNRVTVYLGYLDGDNGSATRNEDKRTTLGLTWMLAQNVEFQFWNTWAS
                     GNRYDSGAGAPATGDQEMGLMLFAGF"
     gene            7156..7542
                     /locus_tag="AC16FV_01615"
     CDS             7156..7542
                     /locus_tag="AC16FV_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848793.1"
                     /translation="MLKSKLAVLGALLALFVIPAVHADDPVRPDPEIRADKKEIVQDR
                     REIRGDKREIRQDVRERNQDRRELRREVREGDKEGAREERRELRQDNAELRGDHRELR
                     QDKRELRQDRRELHRDRKHAQRAKRS"
     gene            complement(7595..7957)
                     /locus_tag="AC16FV_01620"
     CDS             complement(7595..7957)
                     /locus_tag="AC16FV_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR2848794.1"
                     /translation="MNAWVINLVGLAVITLIVWWFWLSRPQARRASGAAPIEIIVNSG
                     VYTPARIEVPAGKPVTLRFVRKDPSPCAEKALFDSLGVNADLPLGKPLDVVVTPPQPG
                     EYEFTCQMRMYRGMLVAR"
     gene            complement(7954..>9394)
                     /locus_tag="AC16FV_01625"
     CDS             complement(7954..>9394)
                     /locus_tag="AC16FV_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR2848795.1"
                     /translation="IIEGHSSVDESMLTGEPMPVEKKTGDEVVGGTFNKSGTFLFQAK
                     RIGADTALARIIEMVRRAQSTKPAIGRLADKISAVFVPSVLIIAVITFLAWFNLGPEP
                     KLSYMLVATMTVLIIACPCALGLATPISIMVGVGKAAEYGILIRNGEALQQAGKLTTI
                     VLDKTGTVTEGRPAVTALVPMPGWDESKLLTVAASIETGSEHPLAEAIVSAARIRQLK
                     TSPANDFQAFTGHGVSAIVDGKKILFGNARLMAQHNIDLGNLAVRAEELAGRAQTPMF
                     LAVDGRAAGIVAVADPIKPDSKAAIARLHAIGLKVAMITGDNRATADAVAREVGVDEV
                     MAEVLPGDKADKVSVLQQRGEIVGMVGDGINDAPALAQADVGYAIGTGTDVAIESADV
                     TLMRGSLHGVADAIAISRATVRNIKQNLFGAFIYNVSGIPVAAGVLYPFTGLLLNPII
                     AGAAMAMSSVTVVSNANRLRWFKSGRLQQ"
BASE COUNT         1981 a         2823 c         2689 g         1901 t
ORIGIN      
        1 tcgatcatta aaagcatata tgaatactcg ccgcagagac gcagagaacg cagagtaaat
       61 cagaaattgt taaccacacg ccgaacacca tctttgagct gtgcaacatt gaaattgatc
      121 agcagaccga gcttcagccc gctcatccgc agatatgaca ataattggga ggtgaatata
      181 ggcaataatg tctcaactgc tttgagttcc acaatcacac tccgctgaac caagagatca
      241 acgcgataac ccgaggagat ctctgtctct ttgtatcgaa cggggaatgc aacctccctc
      301 tccacggcaa gcccgcgtaa accaagctca tgccacaaac aatcccgata aacagactcg
      361 aggagtccgg gaccgagatg tctgtgcacc tcaatggccg ccccgattat ctcgcccgtg
      421 atagagttgt catccatgaa ttctctgcgt cctctgcgtc tctgcggcag ataatcttat
      481 aagcctttcg acaccatggc cacggcgcgg aaaatcgcgc gcgccttgtt catggtctcg
      541 ttccactcgt tgcgcgggac cgagtcggcg acgatgccgg cgccggcttg tatgtaaagc
      601 gtcttgtctt tgatcacggc ggtgcgaatg gcgatggccg tgtccatgtt gccgttccag
      661 ccgatataac caaccgcccc ggaataaatt ccacggcgcg tgggctcgag ctcctcgatg
      721 atttccatgg ctcgcacctt gggcgccccg ctcacggtgc cggccggaaa ggtggcgcgc
      781 aaaacgtcga tcgcgtccag gccgggcttc aactcggccg ttacgttgga gacgatatgc
      841 atgacatgcg aataacgctc gatgaccatt ttatccgata cccgcacgct ccctaccttg
      901 gccacccgtc cgacatcgtt gcggcccagg tccaccagca tgatgtgctc ggcgcgttcc
      961 ttgggatcgg ccagcagttc cttttccagc gcctgatctt ccggttccgt cgcgccgcgc
     1021 gggcgcgtgc cggcgatcgg gcgcacggtc accagcccgt cttccaggcg cacgagaatt
     1081 tccggcgagg agccgacgat atgaaaatcc ttcaggtcca tgaagtacat gtacggcgag
     1141 ggattgagcg tgcgcaacgc gcggtagaga tcgagcggcg cggcggcaaa aggaatcgcc
     1201 aggcgctggg acaacaccac ctgcatgatg tcaccggcgc ggatgtattc gagcgaacgt
     1261 tccaccgcct tctcgaatcc ctgctgcgtg aaacccgaaa taaaatcggc ttccgccacg
     1321 gccttgttgc tctccggcgc cacggcccgc ggaatggatt tgctcaaacc ggtcaccagt
     1381 tcgtccaggc gcgcgttggc cttggcgtag gcatcggcga tggccggatt cacatggatc
     1441 acgacataga gccggccctg caggttgtcg aacacgacga cttcgtccga aaccatgagc
     1501 agtatgtccg gcgcgccgat ggcgtcaggc ttggtcatct ggcccaggcg cggttccaca
     1561 tagcggatgg tctcgtagcc gaagtagccg accagtccac cggtaaagcg cggcagcccg
     1621 tgaatttcag ggacagtgta ttgcgcccgg aaccggcgca cggtttcgag cgggtcggta
     1681 acggtttggc gctggatgac cttgccgtcc ttttcgacgg ttacttcgtt accggaaatg
     1741 cgcaacaggg tgcgcgcggg gagtccgatg atggagtaac ggccccattt ctcgccgccc
     1801 tgaaccgatt cgaacaaata cgaatacggc ccgcgcgcga gcttgaggta aatgcttaag
     1861 ggggtgtcga ggtcggcaag gacctcgcgc atcagcggga tgcggttgaa accttgagcg
     1921 gcgtggttat cgaattcttt tttgcttaac gacatgaatc accatgagat caccagttaa
     1981 acggtcagac agcaacacga gcgatagcca agggccacca ccagcgcaga tccgaccatt
     2041 tacgtattct gtgtctcatg ggtgtcgtta agtataaagc cgcagatact gcggcacctc
     2101 ggtcagcgaa tccaccacgg catcgggcag agactgacgg atatcgtgcc cgtgattata
     2161 gccataagtc acacaaatga caggcatccc cgcggaacgc gcggcgtgca cgtcgttgct
     2221 cgaatccccg accagcaccc cgtgcgaggt atcgatgccg aaatgccggc aggcgtgttg
     2281 cagcggcagg ggatcgggct tctgtttcgg caggctgtca ccggacagca ccagtttgaa
     2341 atacttatgc agctcaagat ttttcaataa agggagcgtg aaggcctcgg ccttgttggt
     2401 gagaccggcc agcgaatagc cctgggcggt gagctgttcg agagcctcga ccacgccggg
     2461 atagggacgg ctcagatcgg acacggcctc gccgtagtgg cggcgaaaca gcgcgagcgc
     2521 gcgttcgttg agttcagcct caggttcggc ctgcatctcg ccggtgagcg cgcgtttcac
     2581 gaaacgcggc acgccgttgc cgatccaggc acgataatgc tcaatgcccc aggcgggacg
     2641 gccaagatca gcgagcatgc gattggccgc cacggccaag tccggcaccg tatcgatcag
     2701 ggtgccatcg aggtcgatca tgaccatctt cacggccaac gggaaactcg ctatcttgcg
     2761 ggccgtcgtt ggtgtcatgc cttggccttg gcaagctcgt cgcgcatcgc cttcacgacc
     2821 gagttgtagc ggttcgggtc gccggccttt ccggcattga agatggcgga accggccacg
     2881 aaagtgtcgg caccggcttc ggcgatctcg cgtatgttgt ccaccttcac cccaccgtcg
     2941 atctcgaggc ggatctggcg cccgctggcg tcgatcattt ttctcgcctc gcgcagcttg
     3001 tcgagcgacg acttgatgaa gctctggccg ccgaatcccg ggttcaccga catgagcaga
     3061 atcatgtcca ccttgtccat cacatacttc agataatcca gcggcgtggc cgggttgaac
     3121 accaggcctg atttgcaacc tgaatcgcgc accagttgca ggctgcgatc gatatgctcg
     3181 ctgccttccg gatgaaacgt aatgtaagtt gcaccagcct tggcgaaatc cgggatgatg
     3241 cgatccaccg gttttaccat caggtgcacg tcgatgtcgg ccttcacgcc atgcttgcgc
     3301 agcgcctcgc ataccagcgg gccgatggtc aggttgggca cataatggtt atccatcaca
     3361 tcgaagtgga cgatgtcggc accggaagcc agcacattcg tgacttcctc acccaggcgg
     3421 gcaaagtcgg ccgacaggat ggatggagca attttgtaat cacgcatggt gttgacctta
     3481 tataaataat cttcgtgaga aggcgcgcac tttacctgaa ccgggtgaag ttttcagccg
     3541 tgccgcaccg gttgcatcag gcactttgtg gcggtttgcg tctttatcga cagactcaat
     3601 tggctgatct tatactttgc cgaagcccct acaatcccaa atgaatttac accctcataa
     3661 aaaaatcggg gccatgcttc ggataaaggt aattaggctc ttttggtcaa caaccagcgg
     3721 ctgatattac gcacgctttt gccgatgata cgcgcacctc tctcaagcca ggttttgcga
     3781 tcgcgggcaa gggcctgcat ttctgtttcg gtgagcaatt ccggcgcgtg acttttcttg
     3841 aggcgcgcga cagccacgcg atcggcctcg cagaatttcg catacatcca ccgactcgcc
     3901 gggttccagg aacgaccgcg gcggaaatgc gcggcaaaat ccaccacatg cggttcgccg
     3961 tcggcgccga ccagaatatt gccctgactg cgcagatcgc aatgcgccac gccgtactcg
     4021 tgcaggcggt ccacgagacg gtaaaaccgt tcgaagaatt gcggagtcaa tccaccgtgc
     4081 ggtacctcgc gtgcgctggt gccgggaatg tattcgacca aaagcgcatc gggccccact
     4141 cgacgcagca agcgtggaac gccgcgcgcg cctttcagct gttcgagggc acgtgcttca
     4201 cgccgcgccg acagcgggcc caccatgcgc cggaaccagg gatcggccct gccataatcc
     4261 ttcagcaccg cctcctcgct gccgaatctg atcaacaaca cgtccgggcg tgaaccgccc
     4321 ccctgccgga aggttttcac cgcatggtcg cgcaaatcct caagaccgag attttcgagc
     4381 gcaggcatgc gtgaatgata gcaaatcggg cgcgtgggac gtgaaccgta aatcgtgagt
     4441 cgcatgacgc gatctgctac gatgcccgac tcacggttca cgacttacga ttgaagatcc
     4501 atgcccgttg ccatcacgct tcacatcctt tccgcggtag tgtgggtcgg cgggatgttt
     4561 ttcgcctgga tggcgctgcg cccggtcgcg gcgagtctgc tggagccgcc gctgcgcctg
     4621 ccattgtggt cgcaaacctt cgtgcgcttc ttcccgtggg tctgggcggc ggtggtactg
     4681 ctgccggcga gcggctactg gatgatcttc gaggtgttca aaggcatgca caatgcaggg
     4741 ctctatattc atgtcatgca ggtcatcggc atcatcatga ttacgatctt cctgcatctg
     4801 ttcttcgtcc cgtaccggcg catgaacaaa gcgatcgcgg ccagcgattt ccccgccgcg
     4861 ggaaaacatc tggctgcgat acgaaaactc atcggcatca atctcgtgct tggcctgatc
     4921 accgtcactg tcgcctccgg cggcgcttac ctgggctagt gaaatgaaaa aacttgttct
     4981 tccgttcgtc tcattcgcct ggctggcttt tctccatggc tgtgctccgc tccgatccaa
     5041 tcccgacatg atccccgtcg aatccgaccc gtccggcgcg gaggtttacg tcatggggaa
     5101 gaaagtcggc atcacaccgc ttgaggtcag gcagatcgat gtctttcctc tgacgtacag
     5161 cccggagcag caggatcttt atggaaaaat tgtattgaaa aaggagggct gcacggaaca
     5221 ccgccagccc gtcagcacaa aggtctatgc gaagggagca aaaataaaac tggactgcgg
     5281 cggcgccgct cccgaagccg ccaaagcaac ccgccctgcc gctcccgaaa caagttctgt
     5341 cgaaaaccgc ctgcgccagt taaaagacct tcgtgagaag ggtctgatta ccgaagaaga
     5401 agaaaaggaa acccgccagc gaatcttgaa agacctctga cgcttcgtcc cgagacgatg
     5461 cctctttcgg gcaggcgccg caaccgcaca aagcctctcc gcgtcatttg acgcggtgag
     5521 gccagaatac tcccgcggcc tagaatcccg cgaacagcat caaccccatc tcctggtcgc
     5581 cggtagccgg tgccccggcc ccgctgtcat aacgattgcc gctcgcccac gtgttccaaa
     5641 attggaattc cacattctgc gccagcatcc aggtcaaccc gagggttgtc cgcttgtctt
     5701 cgttgcgcgt cgcggaaccg ttgtcaccgt caaggtaacc caaatagact gtgacccggt
     5761 tcgggattac ccccgcctca cccagcaggg accacgcctt ctggtcgttc gcgcggctgt
     5821 tgaagaagtt cgtcgtaccg ccggcaggcg tggcatcggc cttgccgtag gtgaaataaa
     5881 ggccaacggg cttcccaccc atcgttccct gcgtctgggc atcgacaaac gaggccttgg
     5941 tgtcggtctt gtcaaccgtg ctggtagtgg tgttcattat ttccgaggtg ccgccgaaga
     6001 gctggatccc gaaggccgta tcccagccgc cgatgttggg catgtaggcg ccgcgaaagt
     6061 aattggcgaa cttttcgtgc gcgatgctgg taccaaaggt gggatgccac agggtgtaat
     6121 tcgcgtacca ttggggggcc gaggcgacaa aagcgaaacc tgtggcctcc gacccccccg
     6181 gccgattgcc gccgctgctg ccgacgaact gctgcgcggc cgtggccgtg cggttctcgg
     6241 cgatacggat aaaccgctgc gcgccggtat tcagcagttc catgccataa ggggcgccgc
     6301 cagcatcggt cagaaacggc acgatgccgt aattgacccc ttccgctttc ttgttgaagt
     6361 gaatacggaa ggaattgtaa ttgctggcgg tgtctatcag cgatgcctcg accaggaatc
     6421 ctatatgttc cccggcgcga ccgccgatca gcagcgccgc ctcatccgga aactggatat
     6481 cgccggtatt ggtggccgac gtttttacct cgccattggt tttcacgtac cgcagcttgg
     6541 tgaccaatcc gatattcagg tccaccggta gagacaggtt ttcgtcctcg atcgtctcct
     6601 gcttgccttt gaacgtgtag gcctcctgct tgaaggcacg gccgaaggcg ttcacggcgg
     6661 gataatgctg gaagtggcag gcattgcagg tcatccctgt ttgccgcgca aagcccggat
     6721 tggcctgcgc cgcctgcatc cacacgaaac tccatgtcag gagcactgct gaaaccacgt
     6781 atttaagtgt tttcatgacg ctctcctttc ccttatttaa cttaatgagg aaccccgagg
     6841 caccgccaat caaactggat ccacagtagg tactgcgaca attcgtcgca ttgatatgaa
     6901 tcaattggca ggtatttgca gtcaaagcag gtcaaacatc cgataagaac cacggggcaa
     6961 ggaaccctaa aagcagatgt tagccattga ttcaaaaaaa acttcttccg gactgtcaac
     7021 gcccgagtac acaaggcgtt agagatcaga gcatgtagaa ggatgagatt acaggtttat
     7081 ctacaatttc tgtcatgtgg acttgctgtg atgggctgta aaaatctttt catctcataa
     7141 ctggagaaat gcatcatgtt gaaatcgaaa cttgccgttc tgggggcgtt actggcgctg
     7201 ttcgtgattc ctgctgtaca tgccgacgat ccagtcagac ccgatccgga aatccgggcg
     7261 gacaaaaaag agatcgtgca ggatcgccgg gaaattcgcg gcgacaagcg tgaaatccgt
     7321 caggatgtcc gcgagcgtaa ccaggaccga cgcgaactgc gccgggaggt ccgcgagggc
     7381 gacaaggaag gtgcgcgcga ggagcggcgt gaactccggc aggacaatgc cgaactgcgc
     7441 ggcgaccacc gggaattgcg ccaggataaa cgcgagctgc gccaggatcg tcgcgaactc
     7501 catcgtgatc gcaaacacgc acaacgcgcc aagcgctcat aaaaccagcg acataaaaaa
     7561 cggggcctga ggccccgttt tttatgtccg gtttttatcg tgccaccagc atcccgcggt
     7621 acatgcgcat ctggcaggtg aattcgtatt cgcccggttg gggcggtgtc acgaccacat
     7681 ccagcggttt gcccaacggg agatcggcgt tcacgccgag gctgtcgaac aaggccttct
     7741 ccgcacaggg gctggggtcc ttgcgcacga agcgcagcgt caccggcttt cccgccggta
     7801 cttcaatgcg cgcgggggtg tagacaccac tgttgacaat aatctcgatc ggtgcggcgc
     7861 ctgatgcgcg tcttgcctgc ggccgcgata gccagaacca ccagacgatc agcgtgatga
     7921 cagccaggcc gacaagattg atcacccagg cattcattgc tgcagccttc ctgacttaaa
     7981 ccatctcaga cggttggcat tgctcaccac cgtcaccgag gacatcgcca tggcagcgcc
     8041 ggcgatgatc ggattgagca gcaggcccgt gaagggataa agcacgccgg cagcgaccgg
     8101 gatacccgac acgttgtaga tgaaagcacc gaacaggttc tgcttgatgt tgcgcacggt
     8161 ggcgcgcgaa atcgcgatgg catcggcgac gccgtgcaag gaaccgcgca tgagcgtcac
     8221 gtccgcggat tcgatggcga catcggtccc ggtgccgatg gcataaccca cgtcggcctg
     8281 cgccagcgcc ggcgcgtcgt tgatgccgtc gcccaccatg ccgacgatct cgccgcgttg
     8341 ttgcaagacc gataccttgt cagccttgtc acccggcaag acttcggcca tgacttcatc
     8401 cacgcccact tcacgggcga ccgcgtcggc ggtggcgcgg ttatcgccgg taatcatcgc
     8461 caccttgagc ccgatggcgt gcaggcgcgc gatggcagcc ttcgaatccg gcttgatcgg
     8521 atcagccacg gccacgatcc cggcggcacg accgtccacg gcgagaaaca tcggagtctg
     8581 tgcgcgcccc gcgagctcct cggcgcgaac ggccagattt ccaagatcga tattgtgctg
     8641 ggccatcagg cgcgcgttgc caaacaagat cttcttccca tccacgatcg ctgaaacacc
     8701 atgaccggta aacgcctgaa aatcattggc cggcgaagtt ttcagctgcc tgatccttgc
     8761 cgccgataca atcgcctcgg ccaacggatg ctccgagccg gtctcgatgc tcgccgccac
     8821 cgtcagcagt ttcgactcat cccagcccgg catgggcacc agcgcggtaa cggccggccg
     8881 gccctcggtg acggtgccgg tcttgtcgag cacgatggtc gtgagcttgc ccgcctgctg
     8941 caaggcctcg ccattgcgga tcaggatgcc gtactcggcg gccttgccca cgcccaccat
     9001 gatcgagatc ggcgtggcca atccgagcgc gcaggggcaa gcgattatga gcaccgtcat
     9061 ggtcgcgacc agcatataag agagcttcgg ttcgggtccg aggttgaacc aggcaagaaa
     9121 agtaatcacc gcaataataa gaaccgaagg cacgaataca gcggagattt tatcagccag
     9181 ccgcccgatc gcgggcttgg tgctttgcgc gcggcgcacc atttcaataa tccgcgcgag
     9241 cgcggtatcg gcgccgatgc gtttggcctg gaacaggaag gtgccggact tgttgaacgt
     9301 gccgccgacg acttcatcgc cggtcttttt ttccacaggc atcggctcgc cggtgagcat
     9361 ggactcgtcc acgctcgaat ggccctcgat gatc
//
LOCUS       DBNGZE010000065         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1676490, whole genome shotgun sequence.
ACCESSION   DBNGZE010000065 DBNGZE010000000
VERSION     DBNGZE010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1676490"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="AC16FV_01630"
     CDS             complement(<1..336)
                     /locus_tag="AC16FV_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2848796.1"
                     /translation="MRRLHLPLFLSLLSLLTACAGTVKPEPEADAASPPSAKTDETLP
                     RVALEPQILYDLLLGEIAGQRGQIGAAAATLGKVAQQTRDPRIAERATLASLYAKRYD
                     EALQSARLWT"
     gene            532..2220
                     /locus_tag="AC16FV_01635"
     CDS             532..2220
                     /locus_tag="AC16FV_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR2848797.1"
                     /translation="MNSLFLLLAAAVAFVFGYRFYSRLLAFGVFRLENNYSIPAPRPT
                     EDESRTAKRHLIFGHHLASLAASAAVTGSMVSLIWGWVPAFLWAVVATVTAAGTYGLG
                     SLWLSLRYPGLNPAEIAARLLGAPAHTLLALFAFLPLLLMNAACATLAAQLLSGFPQA
                     VLPFWTIVVLAWFLGGYLRGRKDFEIISASLVALALCLMAVWLLGEFPLALTGALNFE
                     SKASYLAFDANMAWVLILFVYGYHATRQPMWKLIRPRGFLTALLLGVMLFICYTAVVI
                     DHPVLVAPEFHAGPDTPATLPWLFVTLTSGAVAGFHLLIANGITARQIKREADARYIG
                     YGGALALGLLALSAVVIGATGFANTQEWSQYYASWETLRDLHKVLDLYINGFARLAGG
                     IGLDAAFARTLGAVVIVSLLITTLEAGLRVQKQLLTGLTERFPSLLPGNEKTQIAAVV
                     ILSAALALHDGRGLGGLAGWPLFGLADQLLAVLGFLLLALALRRNGRPVIWVVVPLIF
                     MAAVVHWAFVTLALQWWSAGQWLLFGLGMLLLAMELALALLALNTLKPSAPPPA"
     gene            2474..>2843
                     /locus_tag="AC16FV_01640"
     CDS             2474..>2843
                     /locus_tag="AC16FV_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR2848798.1"
                     /translation="MHLLAFGINHHTAPVDLREKAAFAPEKLIGALHEVTDNGGASEA
                     AIVSTCNRTELYCGLDPAHEDHVIDWFCHYHHLNRSDIHPYLYRHPDQDAVKHAFRVA
                     AGLDSMILGEPQILGQMKEAF"
BASE COUNT          490 a          880 c          861 g          612 t
ORIGIN      
        1 ggtccacaac cgcgccgact ggagcgcctc gtcgtagcgc ttggcgtaca acgacgccag
       61 cgtggcgcgt tcggcgatgc gcggatcgcg cgtctgctgg gcgaccttgc ccaaggtggc
      121 cgcggccgca ccgatctggc cgcgctggcc ggcgatttcg cccagcagca ggtcgtaaag
      181 aatctgcggt tccagcgcga cccggggcaa ggtttcgtcg gttttggccg acgggggaga
      241 cgcggcatcg gcctccggtt cgggtttaac cgtgccggca caggctgtca gcaacgacag
      301 caaggacaga aacaggggca ggtgcaaacg acgcatacgg gaagaaatca tatatatagt
      361 aagtccaggt ccgtcagggt gacacgaata accgtatgac cgtcgctgcg ggcggatatt
      421 cgcctaccat ttaaaatagt cattacagcc tctaacatcc agaaaacgaa gcaaccggga
      481 atgggacgat atcggaatga tctttggcat ggcaaacagc gggaataaat cgtgaattcg
      541 ctatttctgc tgctggcagc agcggtggcc tttgtcttcg gctatcggtt ctattccagg
      601 cttctggcgt tcggcgtttt ccgccttgaa aataactata gtatacccgc cccccgcccc
      661 actgaagatg agtcccgcac cgccaaacgt cacctgatat tcggtcatca tctcgcctcg
      721 ttggcggcca gcgcggccgt cacgggaagc atggtttcct taatatgggg atgggtaccg
      781 gcatttctat gggccgtggt ggccaccgta accgccgccg gcacctacgg gctcggctcg
      841 ctgtggttgt ccctgcgcta tccgggcctg aacccggcgg aaatcgcggc gcgcctgctc
      901 ggtgccccgg cgcatacgct gctggccctg ttcgccttcc ttccattgct actcatgaat
      961 gccgcgtgcg ccacgctggc cgcgcaactg ctgtccggtt tcccacaagc ggttctgcct
     1021 ttctggacga tagtcgtgct ggcgtggttt ctcggcggct acctgcgcgg ccggaaggat
     1081 ttcgaaatca tctccgcctc gctggtcgcc ctcgccttgt gcctgatggc tgtgtggctg
     1141 ctgggagaat tcccgctggc gcttaccggc gcgcttaatt ttgaaagcaa ggcaagctac
     1201 ctggcgtttg acgccaacat ggcctgggtc ctgatcctgt tcgtctacgg ttaccatgcc
     1261 acgcggcaac cgatgtggaa actgatacgt ccgcgcggtt tcctcacggc cctgttgctc
     1321 ggcgtcatgc tcttcatatg ttacaccgcg gtggtgatcg atcatccggt cctggtcgcg
     1381 ccggaattcc acgccggacc ggataccccc gcgacgctcc cgtggctgtt cgtcacgctc
     1441 acctcgggcg cggtcgcggg atttcacctg ttgatcgcca acggcatcac cgcgcgccag
     1501 atcaagcgcg aggccgatgc gcgctatatc ggttacggcg gcgcgctcgc gctcggactg
     1561 ctggcgctga gcgccgtcgt catcggcgcc accggcttcg ccaacacgca ggaatggagc
     1621 cagtattacg cctcgtggga aaccttgcgc gacttgcaca aggttctcga cctttatatc
     1681 aacggcttcg cgcgcctggc cggcggcatc ggactggatg cggcattcgc ccgcacgctg
     1741 ggcgcggtgg ttattgtgag tcttctgatc accacgctgg aagccggcct gcgcgtgcaa
     1801 aaacaattgc tcaccggact gacggaacgc tttccgtcgc tgctgcccgg aaatgaaaaa
     1861 acccagatag ctgccgtcgt catcctgtcg gcggcgctcg cactgcacga cggccgcggt
     1921 ctcggcgggc tggcgggctg gccgctgttc ggcctggccg atcaacttct ggcggtgctc
     1981 ggctttttat tgctggcgtt ggcgctgcgc cgcaatgggc ggccggtgat ctgggtcgtc
     2041 gtgccgctga ttttcatggc ggcagtcgtt cattgggcct tcgtcacgct ggcattgcag
     2101 tggtggtcgg ccggtcagtg gctgctgttc ggtctcggca tgctgctact ggcgatggaa
     2161 ctcgcgctgg ccctgctggc gctgaacaca ttgaaaccgt ccgcgccgcc cccagcttga
     2221 caccggaccc cgatgggtca aaatccccgg catcgagggc catttcccgg ttattgatcc
     2281 ggcacgatgg ttacggaagc gctgggagtc cgtggtgatt gagcgggtgg agttttttga
     2341 gtgccggatc aataacaaaa cctcttaaga ttgccaatta ttgcggcagc aaggcgtctt
     2401 gggttcgggg gtcacaatcc ttgtttaccg gggtttatat tttaaatcgt ttcatgccgc
     2461 cgcaataatc tgcatgcatc tgctcgcctt tggcatcaat caccataccg cgccggtcga
     2521 tctccgcgag aaggcggcgt tcgcgccgga gaaactgatc ggcgcgctgc atgaggtcac
     2581 cgacaacggc ggcgccagcg aggccgccat cgtctccacc tgcaatcgca ccgaactcta
     2641 ctgcggtctc gatccggcgc acgaggatca tgttatcgac tggttctgcc actatcacca
     2701 cctgaaccgc tccgatatcc atccttatct ctaccggcat ccggaccagg atgcggtcaa
     2761 gcacgcgttt cgagtcgcgg ccggcctcga ctcgatgatc ctgggcgaac cgcagatcct
     2821 cggacagatg aaagaggcct tcg
//
LOCUS       DBNGZE010000066         4609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1120629, whole genome shotgun sequence.
ACCESSION   DBNGZE010000066 DBNGZE010000000
VERSION     DBNGZE010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1120629"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1180
                     /locus_tag="AC16FV_01645"
     CDS             206..1180
                     /locus_tag="AC16FV_01645"
                     /inference="COORDINATES: protein motif:HMM:NF022825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HIR2848799.1"
                     /translation="MTAMSVSLSWAETGSRTVRPLYSFNQSPLIQIYGLPALGESRVL
                     SPDESTLAVHLQIANHFTGANTSSENLSLDGETRRLMLNWRQGLAGNREWGFELPYVT
                     HNGGFLDMSIEEFHDIFGLPQNGRTDLPRNRIDFRYSHNGVNIVNLDHSVSGTGDVRL
                     FAGKPVVLETDSSGYTAVWRASLKLPTGDDAELLGSGSTDFATWLSAATTRPPDKWNL
                     YGGGGLLLMSEGDVMPAQQRHAVIFGTFGLSQKFFPQVTINAQLDVHSPFYNDSGLRQ
                     LGEYAMLGLLGMDWEFEARKFVTFSMSEDLAVGASPDVAFNLSLTLPF"
     gene            complement(1193..2116)
                     /locus_tag="AC16FV_01650"
     CDS             complement(1193..2116)
                     /locus_tag="AC16FV_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010213664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2848800.1"
                     /translation="MKLLVTGGAGFIGSHLVERLLATGDRVRVVDDLSTGKRENLVGH
                     EALEFIEGDIRDAGLIDRCTKGVDAVAHLAAVASVQASMDDPVRTHQVNFDGTLNLLE
                     ASRRHGVKRFIYACSAAVYGDTVTIPVAEETMLKPMSPYAVDKLSGEHYLLHYHRTYG
                     LGATSFRFFNIYGPRQDPSSPYSGVISIFVERLKRSQPVTVYGDGTQTRDFVYVADLA
                     DLLARAARGMEGDGGVFNVGTGTRHSLLQLLANLEKISGKKIERRHEPARPGDIQHSC
                     ADVTRLRKTFGNAPATPFEHGLKKLLESLTV"
     gene            2503..4443
                     /locus_tag="AC16FV_01655"
     CDS             2503..4443
                     /locus_tag="AC16FV_01655"
                     /inference="COORDINATES: protein motif:HMM:NF020700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2848801.1"
                     /translation="MEIISGPRLAFGNSVAFPPLVILLLFFFAIFLTACGGGASDSGN
                     TPTASSTSTTTSGSSGGSSSGGSGDTTRPSVAITSPVNSSSYTSLNSTLSLGGTAADN
                     VGVTQVTWSNSLGGSGSASGTGSWSVGSIALQNGTNTITVTARDAAGNTGIDTLIVTY
                     SSGATSRTTYYLSPTGNDSSDGRSPSSPWKSFAKAFGSMVAGDELILLDGTYSATAGT
                     GYINYQGTGSAQPPSGTNLNNVTYVHALNPGNVTIQGGLFIGRSTRKDSFIKVQGITF
                     EGGGQLYNTSYVTVKDCGFHGSFAIGTNDHDNGNDYDLVEDVWIWTSGARIIAINYRS
                     HHNVWRRVVVRGDGCGTSACSGPGNPNVGITVYNSMDVSLQNVMVVDRILAATDEPYG
                     DFAVAQHAPGPDFGRAEWLGTLSLNSPDTGYYMEPDVGGTVDPTIRISNAVAWDAAGW
                     GFNIARSGTNNLLENLTAYARAGDGVRIAPELTSGTLRNVVSVNAGRYGINSAYQPSY
                     ANIYNARSGAYSNTSCSTGCYISNPLADGVTASLKYIVRVESGSLLKTLGLGGTAIGA
                     NVFNRYGSDGTRFGDAGYNTLTSTALWPWPNEDRIKREMCANTTRGFCSTGQRLGNLG
                     PVTLTTYIWEYLGSPIPTGVYP"
BASE COUNT          976 a         1427 c         1274 g          932 t
ORIGIN      
        1 ccgatacctc ttgcgcgcca gacagagtga tcaggccaat atggatttcc caatcatgaa
       61 tttttgggga tgtacaaaaa taaatggctg gaaaaacaca cggaatatgg cagcattggc
      121 agccaaatat tgcacagatc aataatcaaa agtccccaca atcttgcgct ccaccaatcc
      181 ttcctgtgcc gcccttctgc tgtgcgtgac ggccatgtct gtcagtttgt cctgggccga
      241 aacaggttcc cggacagtaa gaccgctcta cagctttaac cagagccccc tgatacagat
      301 atacggcctg cctgcgctgg gtgaatcgcg cgtgctgagc ccggatgagt ccaccttggc
      361 ggttcacctg caaattgcca atcatttcac cggcgccaac accagctctg aaaacctgag
      421 cctggacgga gaaacccggc gtctgatgct gaactggcgc caaggactgg ccgggaacag
      481 ggaatggggc ttcgaattgc cgtatgtgac gcacaacggc gggtttctgg acatgtccat
      541 cgaggaattc cacgatattt tcggcctgcc ccagaatggt cgcaccgacc tgccacgcaa
      601 ccggatcgat ttccgctaca gccataacgg cgtaaacatc gtcaatcttg accactccgt
      661 cagtggtacg ggggatgtgc gtttattcgc cgggaaaccg gtagttctgg agacagattc
      721 cagcggttat accgctgtct ggcgcgcgag cctcaagctg ccgacgggcg acgacgccga
      781 gttgctgggc agcggaagca cggatttcgc gacctggctg agcgccgcca cgacgcgccc
      841 gccggacaaa tggaacctgt acggcggagg cggcctactg ctgatgagcg agggagacgt
      901 catgccggca caacaacgtc atgcggtcat cttcggaact tttgggctca gccagaaatt
      961 tttcccgcaa gtgacaatca acgcgcaact ggacgtgcac agccccttct acaacgacag
     1021 cggactccgg caattgggcg aatatgcgat gttgggatta ctgggcatgg actgggagtt
     1081 cgaggcgagg aaattcgtaa cattctcaat gtcggaagat ctggcggtcg gcgcctcgcc
     1141 cgacgtggcc tttaacctgt ccctcaccct gcctttctga cagggaatta tttcagaccg
     1201 tcagggactc aagaagcttt ttcaggccgt gctcgaaagg cgtcgccggc gcgttgccga
     1261 aagttttcct gaggcgcgtt acatcggcgc aggaatgctg gatatcgccg ggccgtgcgg
     1321 gctcatgacg ccgctcgatt tttttccctg aaattttttc cagattagcc agcaattgca
     1381 gcaacgaatg gcgtgtcccg gtgccgacgt tgaacacgcc gccatcgccc tccatgccgc
     1441 gcgcggcacg cgccaacaga tcggccaaat cggcgacgta tacaaagtcg cgtgtctgtg
     1501 tcccatctcc atataccgta accggctggc tgcgtttcag gcgttccacg aaaatgctga
     1561 tgacgcccga atagggcgag gatggatcct gtcgcggtcc gtagatattg aaaaaccgga
     1621 aggacgtggc gcccaaacca taggtccgat ggtagtgcag caggtagtgc tcacccgaca
     1681 gcttgtccac ggcatagggc gacatcggtt taagcatcgt ctcttccgca accggaatgg
     1741 tcaccgtatc gccgtagacc gccgcggaac aggcgtatat gaaccgcttc acaccatgcc
     1801 ggcgcgacgc ctccagcagg ttcagggttc catcgaaatt gacctgatgc gtgcggaccg
     1861 gatcgtccat gctcgcctgc accgaggcca cggcggcgag atgcgccacg gcatccaccc
     1921 ccttggtgca acgatcgatt aatccggcgt cacggatatc gccctcgatg aattcaagcg
     1981 cctcatgccc gacaagattt tcacgcttgc cggtggaaag atcgtccacc acccgtacac
     2041 ggtcccccgt tgcgagcagc ctttccacca gatgcgagcc gataaatccg gcgccgccgg
     2101 taactaacaa cttcattttg ttattcctgc tggtatcgat atgtttcgac gggcgcttcg
     2161 cgcgtgccgg tttgagaatt taccatagct gagatcaacc gccagttgac gttgcagatc
     2221 cgtcccggac gtcatttcgc attcgtaaaa tcccttcagc atcggcaaac cggttaccgt
     2281 cgggtaccct gttttttgct ttcatcggga cagttgccat tcatccgtca taactatccg
     2341 taaatcagtt ttcattcctt ttctgccgta tccagctgtt atatttgcga aaagtgcctg
     2401 acaaagcgaa cagcagattc gcgcttgcgg ggtagtgaaa gtcgtcgtgt gtcgttaaac
     2461 gatctcgcgc gcgaggtcta gggaaaggaa aagtaaaact aaatggaaat tatcagcggg
     2521 ccacgcctcg catttggcaa cagcgtagca tttccgcctt tagtcatcct ccttctattt
     2581 ttctttgcca tttttctgac cgcctgcggc ggcggagcga gtgatagcgg caacacgcca
     2641 actgccagca gcaccagcac aaccaccagc ggttcgagcg gcggctcatc gagtggtggt
     2701 tctggcgata cgacgcgtcc cagcgtcgcg atcacttcgc cggtaaatag ctccagttat
     2761 acctctctca acagcaccct cagcctcggc ggcaccgccg cggacaacgt tggcgtcacg
     2821 caggtcacct ggtcgaactc gctgggcggc agcggcagcg cgagcggtac cggttcgtgg
     2881 agcgttggca gcatcgcgct gcaaaacggg accaacacca tcaccgtgac ggcgcgcgac
     2941 gcagccggca atacgggaat cgataccctg atcgtcacgt acagcagtgg cgctaccagc
     3001 cgcacgacct attatctttc gcccacgggc aatgacagca gcgacggccg ctcgccttcc
     3061 agtccgtgga aatccttcgc caaggccttc ggcagcatgg tggcgggcga tgaactgatc
     3121 ctgctcgacg gaacctattc cgcgacggcc gggacgggct atatcaacta tcagggaacc
     3181 ggctcggccc agccgccctc cgggaccaac cttaataatg tgacctacgt gcatgccctg
     3241 aatccgggca acgtcaccat tcagggcggc ctgtttatcg gccgttccac gcgcaaggac
     3301 agtttcatca aagtccaggg gatcactttc gaaggcggtg gtcagctcta caacacgagc
     3361 tacgtcacgg tcaaagactg cggattccac ggttccttcg cgatcggcac caacgatcac
     3421 gacaacggca acgattacga cttggtggaa gacgtctgga tatggacttc gggcgcgcgc
     3481 atcatcgcga tcaattaccg ctcgcaccat aacgtctggc gccgcgtggt cgtgcgcggt
     3541 gacggctgcg gcacgtccgc ttgcagcggc cccggcaatc ccaacgtcgg catcaccgtg
     3601 tacaactcca tggacgtgtc gctgcagaac gtcatggtgg tcgatcgtat tctcgcggcc
     3661 acggacgagc cttacggcga cttcgccgtg gcccagcacg cccccggccc cgatttcggc
     3721 cgcgccgaat ggctcggcac cctctcgctc aattcgcccg acaccggcta ctacatggaa
     3781 ccggacgtgg gcgggaccgt ggacccgacc atccggattt ccaacgccgt ggcctgggat
     3841 gcggccggtt ggggcttcaa catcgcccgc tccggcacca ataatctgct ggagaacctc
     3901 acggcttatg cccgggccgg ggatggcgtg cggatagccc cggaattgac cagcggcacc
     3961 ctgcgtaacg tcgtctccgt gaatgccggc cgctatggca tcaattccgc gtaccagccc
     4021 agctatgcga acatctacaa cgcccgctcc ggcgcctaca gcaacaccag ctgctcgacc
     4081 ggttgctaca tctccaaccc gctggctgac ggggtcacgg cttcgctgaa atatatcgtc
     4141 cgcgtcgagt ccgggtcgct cctgaaaacc ctggggctcg gcggcacggc catcggtgcc
     4201 aacgtgttca atcgctacgg cagcgacggc acacgcttcg gcgacgcggg ctacaacacg
     4261 cttacatcca cggccctgtg gccgtggccc aatgaagacc gcatcaagcg cgaaatgtgc
     4321 gccaacacca cgcgcggttt ctgttccacc ggccaacgtc tcggcaatct ggggccggta
     4381 actttaacaa cctacatctg ggaatacctt ggtagtccga taccaaccgg agtgtatccg
     4441 taatgtcccg tgggggtgac atgaaggatg actctacctg caatctggag aaatattatg
     4501 cacaaggcta catacctcct cgtcctttcg ttgctcgctg ttctcgtcac tgcctgcggt
     4561 ggcggcgctg acagcaacac cggcgcgacc agcaccacca acaattcgc
//
LOCUS       DBNGZE010000067         4563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1606926, whole genome shotgun sequence.
ACCESSION   DBNGZE010000067 DBNGZE010000000
VERSION     DBNGZE010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1606926"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="AC16FV_01660"
     CDS             complement(<1..409)
                     /locus_tag="AC16FV_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848802.1"
                     /translation="MPHPRKDADPLLIGYLDAIVALTCQREAQGLTASLIDSLCRHVR
                     AERFRLLAILNLNKETEFDDSNMQDAVVRDWLDPLVAPVPIGDDKDLLVCARTKARVS
                     RPLPGGAGRRLILPIFGAAHVTAMLAIEGLREVG"
     gene            640..713
                     /locus_tag="AC16FV_01665"
     tRNA            640..713
                     /locus_tag="AC16FV_01665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:672..674,aa:Gly,seq:ccc)
     gene            732..2447
                     /locus_tag="AC16FV_01670"
     CDS             732..2447
                     /locus_tag="AC16FV_01670"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR2848803.1"
                     /translation="MKKTLKILAILFGGVLALFIVLAVTLTLLFDPNNYKDDIIKVVK
                     EKTGRELRIEGKLGWSFFPWIGFETGRLELANAPGFGPEPFARLDGAGAKVELLPLLR
                     LRVSVDTIFLDGLKLNLAKNAAGKTNWDDLLAPADKEKPAEKPAEKPAAGKKVDITGI
                     SVNKIDIRKADVTWKDQASKTQYAVRNLNLQTGKIVVGEPVDMHLAFDLESGQPSMSK
                     HLDLKSRINLDLKTQKLEVTNLALAFDESRLSGTLGIKNFDKPAYRVDLALDQIDLDR
                     YMPTPAPAAAGAKPGAAAPAQPVEIPLSLLRSLDVQGKLGIQKLKAMNLHSNDVVIQV
                     SAKDGLITLGPNQAKLYNGKYAGRTSLDVRGKTPLLTIDESVSGIELAPALKDALQFD
                     KFTGSANLSAKATAQGLDARQIKQTLNGSASFGVQNGAIKGMDLKKMIDAIEAAKRDK
                     NYQKLAELSPQPGDETSFSQLSGTAQIKNGVVQNNDLKIQSPKLLNISGKGSADLPRE
                     TLDYIVTVGAYPIIIDGPFAKPRFRPDWNAILKGKVEEKKTEIKEKVEQKLEQKLKDK
                     LKLFK"
     gene            2460..3014
                     /locus_tag="AC16FV_01675"
     CDS             2460..3014
                     /locus_tag="AC16FV_01675"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2848804.1"
                     /translation="MGLRATALLALGLWAATSLAATIEVLETTHDNGRYTVSFEVVLD
                     AARDKVWQIMTDYERLPRVSKIITESRVLKREDANRHRVGVTLEACVLIFCKTVKRVV
                     DIEAWPQKEIVVTDDPAFSNFSYAVERWRVAAEGSRTRLNYTAEVVPNFFIPPLIGPP
                     VVKYFLRREIRITALEVEALAKHE"
     gene            3007..4059
                     /gene="mutY"
                     /locus_tag="AC16FV_01680"
     CDS             3007..4059
                     /gene="mutY"
                     /locus_tag="AC16FV_01680"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR2848805.1"
                     /translation="MSKDSLSRRLLAWYDRHGRKSLPWKRKRDAYRIWVSEIMLQQTQ
                     VATVIPYFQRFIARFPDVLSLARAELDEVLHLWTGLGYYARARNLHKAALRIVKEHGG
                     KFPRAFEAVADLPGIGRSTAGAILTLAFDQRHPILDGNVKRVLARYHAIDMPVSKRET
                     EQRLWQLAEKHTPRKRIADYTQAIMDLGATVCTRTKPQCAACPLHKTCRAFQLGTPQD
                     FPVRVAARKTPVKATQMLMIRDARGRVLLQRRPPAGLWGGLWGFPECADGNARQWCHK
                     MLGINIQTGSPWPTLRHSFSHFHLDITPIPARLVGGTDQAMENAETVWYNVRRPDRRG
                     FSAPVKYLLDELLRKR"
     gene            4059..4331
                     /locus_tag="AC16FV_01685"
     CDS             4059..4331
                     /locus_tag="AC16FV_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR2848806.1"
                     /translation="MARMVKCVKLGKEAEGLDYPTYPGELGKRIWENVSKEAWKMWQN
                     HQTMLMNEYRLNPLDPKARKFIEQEMEKFLFGTGSKPPEGYVAPSK"
     gene            4415..4487
                     /locus_tag="AC16FV_01690"
     tRNA            4415..4487
                     /locus_tag="AC16FV_01690"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4448..4450,aa:Phe,seq:gaa)
BASE COUNT         1052 a         1368 c         1310 g          833 t
ORIGIN      
        1 cgccaacctc gcgcaacccc tcgatcgcca gcatggcggt gacatgggcc gcgccgaata
       61 tcggcagaat cagccggcgc ccggcgccgc cgggcagagg gcgactcacc cgagccttgg
      121 tgcgggcaca gacaagcaga tccttgtcat ccccgatcgg aaccggggcg accaaggggt
      181 cgagccaatc gcgcacgacg gcgtcctgca tattgctgtc gtcgaattcg gtctctttgt
      241 tgagatttaa aatcgccaac aggcggaatc gctccgcccg cacatggcgg cacaaggaat
      301 cgatcagcga cgcggtgagt ccttgcgcct cgcgctggca agtcagcgcc acaatcgcat
      361 cgagataacc gatcaacaat ggatcggcat ccttccgcgg gtgcggcatt atgtattggg
      421 ctgtcaagac ggaattcact ccctgcgagc tggcctcagc ttgcactttt tcactacatc
      481 aatgcaaaag ctgggccatt tgcggcagat tacttagccc cgtagaatca gctacctagg
      541 cgggtatagt tcatagttca atggcggagc ctggcttccc aggctactaa cacggtttcg
      601 accagatttg cgggggtgag gcggaaccgg cataagtagg cgggtgtagt tcaatggcag
      661 aactgtagct tcccaagcta ctaacgtggg ttcgattccc atcacccgct ccatctccag
      721 gaggaaatac cttgaaaaaa accctgaaaa ttctggccat cctgttcggc ggcgtgctgg
      781 ccttgttcat cgtcctcgcc gtcaccctga cgctgctatt tgaccccaac aattacaagg
      841 atgacatcat caaggtagtg aaggaaaaaa ccggtcgcga gttgcgtatc gagggcaaac
      901 tcggctggtc gttcttcccc tggatcggct tcgaaaccgg ccgcctcgag ctggccaacg
      961 ctcccggctt cgggcctgaa ccgttcgcgc gtctggatgg cgctggcgcc aaagtcgagc
     1021 tgctgccgtt gctgcgcctg cgtgtgagcg tggacactat ttttctcgac ggcctcaagc
     1081 tcaatctcgc caaaaacgcc gcgggcaaaa ccaattggga cgatctgtta gctcccgccg
     1141 acaaggaaaa gcctgccgag aaaccagccg agaagcccgc agcgggcaaa aaagtcgaca
     1201 tcaccggcat cagcgtcaac aagatcgaca tccgcaaggc ggacgtgacc tggaaagatc
     1261 aggccagcaa gacgcaatac gccgtacgca acctcaatct tcagactgga aaaatcgtcg
     1321 tcggcgaacc ggtggacatg catctcgcat tcgatctgga gagtggacaa ccatcgatga
     1381 gcaaacatct ggatctgaaa tcccggatca acctcgacct caagacccag aagctggagg
     1441 tcacaaatct ggcgctggct tttgatgaaa gtcgtttgag cggaacactc ggcatcaaga
     1501 atttcgacaa gccggcctac cgcgtcgact tggcgctgga ccagatcgac ctcgaccgtt
     1561 acatgccgac gcccgccccg gccgcggccg gtgcgaaacc cggtgcggcg gcaccggccc
     1621 agccggtgga gataccgttg agcctgctgc gctcgctcga cgtccaaggc aagctgggca
     1681 ttcagaaact caaggcaatg aacctgcact ccaacgacgt cgtgatccag gtcagtgcca
     1741 aagatggttt gatcacgctc ggtcccaacc aggccaagct ttacaacggc aagtacgccg
     1801 gccgcacctc gctcgacgtg cgcggcaaga cgccgttgct gaccattgac gaatccgtca
     1861 gcggcatcga gctggcgccg gcgcttaaag atgccctcca attcgacaaa ttcaccggct
     1921 ccgccaacct gagcgccaag gccacggccc agggcctgga tgcccggcag atcaaacaaa
     1981 ccctgaacgg ttccgcatcg ttcggcgtgc agaacggcgc gatcaagggc atggacctga
     2041 aaaaaatgat cgatgccatc gaagccgcga agcgcgacaa gaactatcag aaactggccg
     2101 agctttcgcc acagcccggc gatgaaacca gcttttccca gttgagcgga acggcgcaga
     2161 tcaagaacgg cgtggttcag aacaacgatc tgaaaatcca gagtccgaag ctattgaata
     2221 tcagcggcaa aggctcggcc gatttgccca gggaaaccct cgattacatc gtcaccgtgg
     2281 gcgcttaccc catcatcatc gacggaccct ttgcgaaacc gagattcaga ccagactgga
     2341 atgctattct caaaggcaag gtcgaggaaa agaaaaccga gatcaaggaa aaggtcgaac
     2401 agaaactgga gcaaaagctg aaagacaagc tcaagctgtt caaatagcgg caacgaaaca
     2461 tggggcttcg tgcaacagcc ttactggctc tgggtctgtg ggccgcaacc tcgctggccg
     2521 ccacgatcga ggtgctcgaa accacgcacg acaacggccg ctacaccgtg tccttcgagg
     2581 tcgtgctcga cgccgcgcgc gacaaggtct ggcaaatcat gacggattac gaacgcctgc
     2641 cgcgggtgtc gaagatcatc accgagagcc gggttctgaa acgggaggac gccaaccggc
     2701 atcgcgtcgg cgtcacgctc gaggcctgtg tcctgatctt ctgcaagacc gttaaaaggg
     2761 tcgtggatat tgaggcttgg ccacaaaagg aaatcgtcgt gaccgacgat ccggccttca
     2821 gcaacttcag ctatgccgtc gagcgctggc gagtggcggc cgagggctcc agaactagat
     2881 tgaattacac cgccgaagtg gtgcctaatt tttttatccc gcccttgatc ggcccgccgg
     2941 tggtgaaata cttcctgcgc cgggaaatcc ggatcaccgc cctcgaggtg gaggcattgg
     3001 ccaagcatga gtaaagattc actaagccgt cggttgcttg cttggtacga ccgtcatggc
     3061 cgtaagagcc tgccgtggaa acgcaagcgc gatgcgtacc gcatctgggt ctcggagatc
     3121 atgctgcaac agacgcaggt cgcgacggtc attccgtatt tccagcgttt catcgcgcgc
     3181 tttcccgatg tcctgtcgct ggcgcgcgcc gagctcgatg aggtcctgca tctctggacc
     3241 ggcctcggct attacgcgcg ggcacgcaat ctgcataaag cggcgctgcg tatcgtgaag
     3301 gagcacggcg gcaaatttcc acgcgccttc gaggcggtgg cagatttacc cggtatcggc
     3361 cgttccactg caggtgcgat cctgacgctg gccttcgatc aacgtcatcc gatactcgat
     3421 ggcaacgtca agcgcgtgct ggcgcgttat cacgccattg atatgccagt aagcaaacgc
     3481 gagacggaac agcgcctgtg gcagcttgcg gaaaagcaca caccgcgcaa acgcatcgcc
     3541 gactacacgc aggcgatcat ggatttgggc gcaaccgtgt gcacgcgcac gaaacctcag
     3601 tgcgctgcat gtccgctaca caagacttgc cgcgcgtttc agctgggcac gccgcaggat
     3661 tttccggtgc gcgtggcggc gcgcaagacg ccggtcaagg ccacgcaaat gctcatgatc
     3721 cgcgatgcgc gcgggcgcgt gctgctgcaa cgccgcccgc cagcgggact gtggggcggt
     3781 ctgtggggct ttcccgagtg cgccgatggc aatgcacggc aatggtgtca caagatgctg
     3841 ggcatcaaca ttcaaaccgg atcgccatgg ccgacactgc gccacagctt cagtcacttt
     3901 catctcgaca tcacgccgat acccgcgcgg ctggtggggg gaacggatca ggcgatggag
     3961 aatgccgaaa cagtctggta taacgtgcgc cgaccggatc ggcgcggatt cagcgcgccg
     4021 gtgaaatatc tgctcgatga attattgagg aaacgttaat ggcccgcatg gtgaagtgcg
     4081 tcaagctcgg caaagaggcc gaaggactgg attacccgac ctatcccggt gagctcggca
     4141 agcgcatctg ggagaacgtc tcgaaggaag cctggaagat gtggcagaac caccagacca
     4201 tgctgatgaa cgaataccgc ctcaatcccc tcgaccccaa ggcgcgcaaa ttcatcgagc
     4261 aggaaatgga aaaattcctc ttcggcaccg gctccaagcc gcccgaaggt tacgtcgccc
     4321 cgagcaaata atttactcca atcggctgaa atcagggcat tcccgctttt gaattaagcc
     4381 cgggggctcc cctataatgc ccctcccttt caccggccgg gtagctcagt tggtagagca
     4441 gcggactgaa aatccgcgtg tcggcagttc gattctgccc ctggccattg ctatggaacg
     4501 agttagcggc gtcgagcgac gccgcctttt cgccagtgta ccccaagtgt agcccaagcg
     4561 aag
//
LOCUS       DBNGZE010000068         5078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_565395, whole genome shotgun sequence.
ACCESSION   DBNGZE010000068 DBNGZE010000000
VERSION     DBNGZE010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_565395"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="AC16FV_01695"
     CDS             <1..1418
                     /locus_tag="AC16FV_01695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2848807.1"
                     /translation="LPLLHRLSSQPQGARRVVRALILTPTRELAAQVEESVRTYGKHV
                     PLKSTVIFGGVGIHPQIDTLRRGVDILVATPGRLLDHVGQRTVDLSHVEILVLDEADR
                     MLDMGFIRDIRKILALLPRKRQNLLFSATFSDEIKQLADGLLNAPTLIEVARQSVAAD
                     LVSQVYHPVDRIRKRELLAHLIHSQGWKQVLVFTRTKHGANRLADQLEDDGISSSAIH
                     GNKSQSARTKALADFKKGEVRVLVATDIAARGLDIDQLPHVVNYELPNVPEDYVHRIG
                     RTGRAGKEGQAVSLVCVDEHEYLRDIQRLLKRDIPKVVIDGFEPNPSIRAEPISHGRG
                     QRGGGRPQGQRQGDGHRRHQQQPQRHAQGRTDDQRARPHSRNERTHSDAQPRSNGQRS
                     HSNPHAHHADQRQHAPHHTRHTSAPHANSHAPRGEVDGNRRLPDDTSGHGQVRSSFSK
                     TDKPSRRSRPTAGKGTVRRSW"
     gene            1593..3221
                     /locus_tag="AC16FV_01700"
     CDS             1593..3221
                     /locus_tag="AC16FV_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HIR2848808.1"
                     /translation="MITTANITMQFGAKPLFENISVKFGNGNRYGLIGANGCGKSTFM
                     KILGGDLEPTSGNVSVDANERLGKLRQDQFAFENYTVLDTVIMGHAELWRVKQERDRI
                     YGLPEMSEADGMKVADLEHKFAELDGYTAEARAGELLLGVGIPLEQHTGPMSAVAPGW
                     KLRVLLAQALFADPDILLLDEPTNHLDINTIRWLEDVLNARNSTMIIISHDRHFLNGV
                     CTHMADLDYGELRVYPGNYDEYMTAATLVRNQLLADNAKKKAQIAELQTFVSRFSANA
                     SKAKQATSRARQIEKIELAEVKPSSRQNPFLRFDQAKRLYRLALEAKKLSQGYGVTPL
                     FKNLNLSVEVGERVAVIGPNGIGKTTLLRTLVGELPLESGTIKWSENAMLGYYAQDHA
                     ADFATEMSLLDWMNQWKKPGDDEQAIRAVLGRLLFSQDEIKKSTKARSGGEQGRMLFG
                     KLMLQQPNVLIMDEPTNHLDMESIESLNNALEKYPGTLIFVSHDREFVSSLATRIIEM
                     TPRGVENFGGSYDDYLRSQEERAGQIPGRMQKAR"
     gene            complement(3336..3554)
                     /locus_tag="AC16FV_01705"
     CDS             complement(3336..3554)
                     /locus_tag="AC16FV_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848809.1"
                     /translation="MGFSADMSGKCDRHSASYMLLCGGTILLNIQKGKTRRSYITTGH
                     REDEQFIFFGRETRQHNAPLHHKIVFYF"
     gene            3631..4023
                     /locus_tag="AC16FV_01710"
     CDS             3631..4023
                     /locus_tag="AC16FV_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2848810.1"
                     /translation="MFSFLDDFKPYGHWLLRIALGSVFVIHGIGNFMNVASFAATLGV
                     PHVAGLLLVVAEIAGGILVLTGGFMGDKMTRLGALMLIPVLLIVMYTMYWKDMSFTLS
                     KVHVVGGMEYLLVLFLISLYMLLKGNKT"
     gene            complement(4218..4565)
                     /locus_tag="AC16FV_01715"
     CDS             complement(4218..4565)
                     /locus_tag="AC16FV_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848811.1"
                     /translation="MRLLPFILTAAVAIGAEAFAVEPAASTLARPYTVKCVASINVWI
                     EVDSASLPTQKPISTESRKRVQFGLIGSTPYRGDSVLCNYATRRRDVATSYSVRCLQP
                     RKEHGYKHSYFCQ"
     gene            4688..4957
                     /locus_tag="AC16FV_01720"
                     /pseudo
     CDS             4688..4957
                     /locus_tag="AC16FV_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
BASE COUNT         1061 a         1582 c         1480 g          955 t
ORIGIN      
        1 cgctgccgct gctgcaccgc ctcagctccc agccacaagg cgcgcgccgt gtggtgcgcg
       61 ccctgatcct gacgccgacg cgtgaactcg ccgcgcaggt ggaggagagc gtgcgcacct
      121 acggcaaaca tgtgccgctc aagtccaccg tcatcttcgg cggcgtcggc atccatcccc
      181 agatcgacac cctgcgccgc ggcgtggaca tcctagtggc gacaccgggc cgcctgctcg
      241 accatgtcgg ccagcggacc gtggatctgt cgcatgtcga aatcctggtg ctggacgagg
      301 ccgaccgcat gctcgacatg ggcttcatcc gcgacatccg caagatcctc gcgttgctgc
      361 ccaggaagcg ccagaacctg ttgttctccg ccaccttctc cgacgagatc aagcagctcg
      421 ccgatggcct gctgaatgcg ccgacgctga tcgaagtagc gcgccaaagc gtcgccgccg
      481 acctcgtgtc gcaggtatac catccggttg accgcatacg taaacgtgaa ttactggcac
      541 acctgatcca ctcgcaaggc tggaagcagg tgctggtgtt cacccgcacc aagcatggcg
      601 ccaaccggct cgccgatcag ctggaggacg acggcatcag ttcctccgcc attcacggca
      661 acaagagcca aagcgcgcgc accaaggcgc tggcggattt taaaaagggc gaagtgcgag
      721 tgctagtagc caccgacatc gccgcgcgtg gactggacat cgaccagctg ccgcatgtcg
      781 tgaattacga actgcccaac gtgccggagg attacgtgca tcgcatcggc cgcaccggcc
      841 gcgccggcaa ggaagggcag gcggtctcgc tggtgtgcgt ggacgagcac gaatacctgc
      901 gcgacatcca gcgcctgctc aagcgcgata tcccaaaggt cgtgattgat ggcttcgaac
      961 cgaatccgtc gatcagggct gaaccgatca gtcatggacg tggccagcgc ggtggcggca
     1021 ggccccaagg tcagcgtcag ggcgatggac atcgccgcca ccaacaacag ccacaacgtc
     1081 acgcgcaggg tcgcaccgat gatcagcgtg cgcgcccgca ttcccgtaac gagcgcaccc
     1141 attcggacgc acagccacgt tccaacggtc agcgttcaca ttcgaatccg catgcccacc
     1201 acgccgatca gcgtcagcat gcgccgcatc acacccgaca cacgtccgca ccacatgcca
     1261 acagccatgc cccgcgcgga gaggtagacg gcaaccgtcg ccttccggat gacacaagcg
     1321 gccacggaca ggtgcgcagc tcgttcagca agactgacaa gccctcgcgc cgttctcgcc
     1381 cgacggcggg caaggggacc gtgcgccgct cctggtgaga tgcaataaat gtggaaatct
     1441 acatttaaaa cctggatgcc cgcctacgcg ggcatgacgg caataaatct gtcattcccg
     1501 cggaagcggg aatccaggtt ttgcatgtca tccctgcgca gcagggattc tacagcagag
     1561 cacaaaatta aattcttgag tggaattgag ccttgatcac caccgccaac atcaccatgc
     1621 agttcggggc caagcctctg ttcgagaaca tctcggtcaa attcggcaac ggcaaccgct
     1681 acggcctgat cggcgccaac ggctgcggca agtcgacgtt catgaaaatc cttggcggcg
     1741 acctggaacc gacctcgggc aacgtttccg tggatgccaa tgagcgcctc ggcaagctgc
     1801 gtcaggacca gttcgcgttc gagaattaca ccgtgctcga caccgtgatc atgggccacg
     1861 ccgaattgtg gcgcgtgaaa caggagcgcg accgcattta cggccttccg gaaatgagcg
     1921 aggccgatgg catgaaggtg gccgatctgg aacacaagtt cgccgagctc gacggctaca
     1981 ccgccgaggc gcgcgccggc gaattgctgc ttggcgtcgg tatcccgctg gaacagcata
     2041 ccggcccgat gagcgccgtg gccccgggct ggaagcttcg cgtattgctg gcacaggccc
     2101 tgttcgcgga cccggacatc ctgctgctgg atgagcccac caaccatctc gacatcaaca
     2161 ccatccgctg gctggaagac gtgctcaacg cgcgcaacag caccatgatc atcatctccc
     2221 acgaccggca ctttttgaac ggcgtgtgca cgcacatggc ggatcttgac tacggtgagc
     2281 tgcgcgttta ccccggcaac tacgacgaat acatgaccgc cgccaccttg gtgcgcaatc
     2341 aattgttggc cgacaacgcc aagaagaaag cgcagatcgc ggagctgcag accttcgtca
     2401 gccgcttttc cgccaacgcc tccaaggcca agcaggcgac ctcgcgcgcg cggcagatcg
     2461 aaaagatcga gctggccgag gtcaagccgt cgagccggca gaatcctttt ttacgcttcg
     2521 atcaggccaa gcggctttat cgcctcgcac tggaggcaaa aaaactgagt cagggctatg
     2581 gcgtgacgcc gttgttcaaa aatctcaacc tgtcagtcga ggtcggtgaa cgcgtggccg
     2641 taatcggccc caacggcatc ggcaagacca cgctgctgcg caccctggtc ggcgaactcc
     2701 cgctggagag cggcacgatc aaatggtcgg agaatgccat gctgggttac tacgcacagg
     2761 accacgccgc tgactttgcg acggagatgt ccttgctcga ttggatgaac caatggaaaa
     2821 aacccggcga cgacgagcag gcgatccgcg ccgtcctcgg ccggctgctg ttctcccagg
     2881 acgagatcaa aaaatcgacc aaggcccggt caggcggcga gcaggggcgc atgctgttcg
     2941 gcaaattgat gctgcaacag cccaacgtgc tgatcatgga cgaacccacc aaccatctcg
     3001 atatggaatc catcgaatcg ctcaacaacg cgctggaaaa atatccgggc acgctgattt
     3061 tcgtcagcca cgaccgtgaa tttgtttcat cgctggcgac gcgcatcatc gaaatgacgc
     3121 cgcggggcgt cgagaatttc ggcggcagct acgacgacta tctccgcagc caggaagagc
     3181 gggcgggaca gataccgggc cggatgcaga aggctcgata atcccggttc ccgctttgcg
     3241 ttaggatttc ggcaacgaaa ctcctttcat ccctgtgagc acgaccagcg ccggcaacag
     3301 tgaccccaca gccgcgaaaa ccagtccgcg tggactcaaa aataaaagac gatcttgtgg
     3361 tggagggggg cattatgctg gcgggtctcg cggccaaaaa atataaactg ctcatcttca
     3421 cgatggccag tcgtgatgta gctcctccgt gttttccctt tttgaatatt caaaagtata
     3481 gtcccgccgc acaaaagcat ataacttgct gaatgcctgt cgcatttgcc gctcatatcg
     3541 gcggaaaaac ccataaaaaa attttctggc agggcgccaa agcgtggcta ttgtttagcc
     3601 acatcatcaa gtactcgtaa ggaggtactt atgttcagtt tcctcgatga cttcaaaccc
     3661 tatgggcatt ggctgctgcg catcgccttg ggcagcgtgt tcgtgattca cggcatcggc
     3721 aacttcatga atgtcgccag ctttgccgcg actctcggcg tgccgcacgt ggcgggcctg
     3781 ctgctcgtgg tggcggagat tgccggcggt atcctggtgc tcacgggcgg gttcatgggc
     3841 gacaagatga cccgtctcgg cgcgctgatg ctgatcccgg tcctgctcat cgtgatgtac
     3901 acgatgtact ggaaggacat gagctttacg ctctccaaag tccatgtcgt gggcgggatg
     3961 gaatatctgc tggtgctgtt cctgatatct ctctacatgt tgctgaaggg aaacaagaca
     4021 taacggccgc gctttagcag gctgtttttt tcggctcccg cccgcgaggg cgggggcttt
     4081 ttttgcgtcc ctcggcaccg caaacggcac cttgcatcct tagctccgta aacggtgcat
     4141 ggcgtcctcg gctccgtaaa cggtacttcc ctataccact cctcatgcac cacttctcct
     4201 gtgccgcggt tttcgcctca ctgacagaaa taggagtgtt tgtaaccgtg ctccttgcgc
     4261 ggctggaggc agcgaacgga ataggaggtg gccacgtcgc gccggcgggt ggcgtagtta
     4321 cacagcaccg aatcgccgcg atagggcgtg gagccgatca gaccgaattg cacgcgcttt
     4381 ctggattcgg tgctgatggg tttttgcgtc gggaggctgg cgctgtcgac ctcgatccag
     4441 acgttgatcg aggccacgca cttcacggtg tagggacggg caagcgtcga cgccgcgggc
     4501 tcgaccgcga aagcttcggc cccaatggcg acggccgcag tcaagatgaa tggaagcaga
     4561 cgcataaaac ctccctgtgt cgtttattga gcctagccga atggcccgtc ccggcaaggc
     4621 cgttggtcag gatattgcgc gcgccggcgt ggtcgtgaaa cgacattcat tagctcataa
     4681 tggctgcatg cgcgaaatag acggctccta cggtgagggt ggtggacagc ttttgcgcac
     4741 ggcctgcgcg ctttccgcca tcaccggcga acctgtccgg ctgcacgaca tccgcgcgcg
     4801 ccgttctcca ccggggctcg cgccacaaca cctcaccgcg gtcaaaaccg tcgcggccct
     4861 gtgtgacgcc catgtcgacg gcctcgagtt gcgatcctcc gagctgatct tccgccctgg
     4921 ctgcttgcgt ggcggcgaat tccgtttcga cgtcggggcc tgaatgtagc aatcacgctc
     4981 tccgtcgttg ccttgctcac tatcctggca cggatcacct acctggacgc gctgttcgtg
     5041 tcggagttca gaaataggct cagcgaggac cagccctg
//
LOCUS       DBNGZE010000069         4004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_287764, whole genome shotgun sequence.
ACCESSION   DBNGZE010000069 DBNGZE010000000
VERSION     DBNGZE010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_287764"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..631)
                     /locus_tag="AC16FV_01725"
     CDS             complement(8..631)
                     /locus_tag="AC16FV_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR2848812.1"
                     /translation="MAQRLKIPPTKSALLSLKRQVSFLEQGHAMLERKRELLTRLVYE
                     RLDQYRKLRAEVRDGLKEAYRWLALAHMRMGSQMLRQSALGFRPAIRLNILPRSSIGV
                     EYPAVEAALLPLQPVGLMWTDASYDEARARLAQTAVGLARLGEAETALWRLLDEQRKT
                     QKRVNALKYNVIPRYRATIRFIQSALEEEERNTLFQIKVLREKGMGA"
     gene            complement(651..2048)
                     /locus_tag="AC16FV_01730"
     CDS             complement(651..2048)
                     /locus_tag="AC16FV_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR2848813.1"
                     /translation="MKEKEYRMSSAARGGLLFLRGVPGVALGDRVVVRDHRGRKRNGQ
                     VIRTSDETVLVQVFEGTDDLDLERTWVRFLEEPFEIPLSRDLLGRIFNGIGAPRDGRP
                     PLVSSLKRNVNGAPMNPAARAYPREFIQTGVSTIDGLNSLVRGQKLPIFSGSGLPHNR
                     LAAQIVRQAKLIGEESRFAVVFAAMGVSYADARFFEEDFESSGVLGNVVMFINLADDP
                     PIERLILPRTALTAAEYLAYDLDLHVLVVITDMTNYAEALREVATAKGDVPSRKGYPG
                     YLYSDLAEIYERAGRVKDRRGSITMVPVLSMPSDDITHPIPDLTGYITEGQIVLSREL
                     HMQGVYPPVNVPPSLSRLMKDGIGKDDTREDHPRVASQLYAAYARALEVRNLASIIGA
                     EELGETDRQYLEFARAFEERFVGQHEDEDRSIIETLTIAWQLLSLLPPEALTRVREAD
                     LAKYHHYEKTAAKVA"
     gene            complement(2045..3877)
                     /locus_tag="AC16FV_01735"
     CDS             complement(2045..3877)
                     /locus_tag="AC16FV_01735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR2848814.1"
                     /translation="MDKRAAGKIVEINGPIVTVSLPRVLNGEQVRIGKLGLVGEVIGR
                     DGEQAVVQVYESTESVRPGEDAEPLGHPLAVELGPGLLGNIFDGVQRPLAAIREQSGD
                     QVARGLNIAPLDRTRTWAFEPSKELARGKPVAGGSVLGTVQETKTIQHRVLVPPDVAG
                     ELVELVAAGEYNIEQVVARVKTADGTTRELKLYHRWPVRQARPYRRRDHAAEPLITGQ
                     RILDTFFPLLKGGKAAVPGPFGAGKTVVQQQIARWSNAEIVIYVGCGERGNELVEILE
                     TFPELTDPYTGRLLMERTMLVANTSNMPVVAREASIYVGVTLAEYYRAQGYDVVLVAD
                     STSRWAEALREVAGRLGQMPVEEGYPAYLASRLAAFYERAGRVETFSGKHGSVSLIGA
                     VSPPGGDFSEPVTSHTKEIVQTFWALSKQLADARHYPSVDWTDSFSGYVNASAKWWHA
                     NVDPRWSERRNEALGLLAQADELARIVNLVGPEALSPAQRWALEGASLVKEGVLQQSA
                     LDEIDSFCSPQKQFLLLDLVLDIYHQGQELLGIGVPVQQLLGLPILARARRVKSSYTS
                     QQLEELKAVRREIQDAFAGLQTEYGKPGEKPKPGEKLKPEEHPK"
BASE COUNT          711 a         1326 c         1220 g          747 t
ORIGIN      
        1 cgccgcctca ggcacccata cccttctccc gcaacacctt gatctgaaac agcgtgttgc
       61 gctcctcttc ctcgagcgcg gactggatga aacggatcgt ggcacggtaa cgtggaatga
      121 cgttgtactt gagggcattg acgcgtttct gggtcttgcg ctgttcgtcg agcaggcgcc
      181 acagcgccgt ctcggcctcg cccagacgcg caagccccac cgccgtctgc gccagtctcg
      241 cccgcgcctc atcgtagctg gcatcggtcc acatcagacc caccggttgc aacggcaaca
      301 gtgcagcctc gaccgcggga tattccacgc cgatgctcga tcgcgggagg atgttcagcc
      361 ggatggcggg acgaaatccc agtgctgact ggcgcagcat ctggctgccc atgcgcatgt
      421 gcgccagcgc cagccagcga taagcctcct tgagcccgtc acgcacttcg gcgcgcagct
      481 tacgatattg atcgagacgt tcgtaaacca ggcgcgtcaa caactcgcgc ttgcgttcga
      541 gcatggcgtg gccctgttcg aggaacgata cctgccgctt gagcgacaac agggcgctct
      601 tcgtgggcgg gatttttaat ctctgggcca tcgggaattt atcggcgttt tcatgccact
      661 ttcgccgccg tcttttcgta atggtgatat ttcgcgagat cggcctcgcg cacgcgcgtg
      721 agcgcttccg gcggcagcag cgacagcaac tgccaggcga tggtaagcgt ctcgatgatg
      781 gaacggtcct cgtcctcgtg ctgaccgacg aaacgttcct cgaaggccct ggcgaactcg
      841 agatactgac gatcggtttc tcccaactcc tcggcgccga tgatcgaggc caggttgcgc
      901 acctcgagcg cgcgggcata ggcagcgtaa agctggctcg ccacgcgcgg atgatcctcg
      961 cgcgtgtcgt ccttgccgat gccgtccttc atcaggcgcg acagcgacgg cggcacgttc
     1021 actgggggat aaaccccctg catgtgcaac tcgcgcgaga gcacgatctg gccttcggtg
     1081 atgtaaccgg tcaggtccgg aatcggatgc gtgatgtcgt cgctcggcat ggacagcacc
     1141 ggcaccatgg tgatggaacc gcgccgatcc ttgacgcgcc cggcgcgctc gtaaatctcg
     1201 gcgagatcgg aatagagata acccggataa cccttgcgcg acggcacgtc acccttggcg
     1261 gtggcaacct cgcgcaacgc ctcggcatag ttggtcatgt cggtgatcac caccagcaca
     1321 tgcaggtcga gatcgtaagc caggtactcg gctgccgtca gcgcggtgcg cggcaggatc
     1381 aggcgctcga tcggcgggtc gtcggcgaga ttgatgaaca tcaccacgtt gccgagcacg
     1441 ccgctcgact cgaaatcctc ctcgaagaaa cgcgcatcgg cataggacac gcccatggcg
     1501 gcgaatacca cggcgaagcg ggattcctcg ccgatcagtt tcgcctggcg cacgatctgt
     1561 gccgcgagcc ggttgtgcgg caggcccgaa ccggaaaaga tcggcaattt ctgaccacgc
     1621 accagcgaat tgagtccgtc gatggtggaa acgccggtct gaatgaactc gcgcggatag
     1681 gcgcgcgccg ccggattcat gggcgcgccg ttgacgttgc gcttgagcga agacaccagc
     1741 ggcgggcgac cgtcgcgcgg cgcaccgatg ccgttgaaaa tgcgtcccaa cagatcgcgc
     1801 gacaagggta tttcgaacgg ttcttcgagg aaccgcaccc aggtacgttc gaggtcgaga
     1861 tcatcagtgc cctcgaacac ctgcaccagc acggtttcgt ccgaggtacg aatcacctga
     1921 ccgttgcgtt tgcgcccgcg gtgatcgcgc accaccacgc ggtcgcccag cgccacgccc
     1981 ggcacgccgc gcaggaacaa caggccgcca cgcgcggcgg agctcatgcg atattctttc
     2041 tctttcattt ggggtgttcc tccggtttga gtttctcccc cggtttcggt ttctcccccg
     2101 gcttgccata ctcggtttgc agcccagcga aagcgtcctg aatctcgcgc cgcacagcct
     2161 tgagttcctc gagctgctgg ctggtgtaag acgatttgac gcgacgggcg cgcgcgagga
     2221 tcggcagccc cagcagttgt tgcaccggca cgccgatgcc gagcaattcc tggccctggt
     2281 gatagatatc caataccagg tccagcagca ggaattgttt ctgcggcgag cagaaactgt
     2341 cgatctcgtc gagcgcgctc tgttgcagca cgccttcctt caccagcgac gccccctcga
     2401 gcgcccagcg ttgcgccggc gacaatgcct ccgggcccac caggttgacg atacgcgcca
     2461 gctcatcggc ctgcgccagc aaccccaggg cctcgttgcg gcgttcgctc cagcgcggat
     2521 cgacgttcgc atgccaccat ttggcggagg cattgacgta accggaaaaa ctgtcggtcc
     2581 agtccaccga cggataatgg cgcgcatccg ccagctgctt ggacagcgcc cagaaggtct
     2641 gcacgatctc cttggtgtgg ctggtgaccg gctcggagaa atcgccgccc ggcggcgata
     2701 ccgcgccgat caggctgacc gagccgtgtt tgccggagaa ggtttcgaca cgtccggcgc
     2761 gttcgtagaa cgcggcgagg cgagaggcga gataggccgg atagccttct tcgaccggca
     2821 tctgaccgag acggccggca acctcgcgca aggcctcggc ccagcggctg gtggagtccg
     2881 ccaccagcac cacgtcgtag ccctgggcgc ggtagtactc ggccagcgtc acgcctacat
     2941 atatagatgc ttcgcgcgcc accaccggca tgttcgaagt atttgccacc agcatggtgc
     3001 gctccatgag cagtcgcccg gtgtaggggt cggtcagctc cgggaaggtc tcgaggattt
     3061 ccacaagctc gttgccacgc tcgccgcaac cgacatagat cacgatctcg gcgtttgacc
     3121 agcgcgcaat ctgctgctgc accacggtct tgccggcgcc gaacgggccc ggcaccgcgg
     3181 ccttgccgcc tttcagcagc gggaaaaacg tatcgagaat acgctggccg gtgataagcg
     3241 gttcggcggc gtgatcgcgc cgccggtaag ggcgcgcctg gcgtactggc cagcggtgat
     3301 acagcttgag ctcgcgcgtt gtaccgtccg cggttttcac ccgcgccacc acttgctcga
     3361 tgttatattc acccgcggcc acgagttcca ccagctcacc tgcgacatcc ggcggcacca
     3421 gcacgcgatg ctgaatggtc ttggtctcct gtaccgtacc gagtacgctg cctccggcaa
     3481 ccggcttgcc gcgcgcaagt tcttttgatg gctcgaaggc ccaggtgcgt gtacgatcaa
     3541 gcggcgcgat gttgagcccg cgcgccacct gatcgccgct ctgttcgcgg atagccgcca
     3601 acggccgctg cacgccgtcg aaaatgttgc cgagcaatcc cggcccgagc tcgaccgcga
     3661 gcgggtggcc gagcggttcg gcgtcctcgc ccggacgcac cgactcggtc gattcgtaga
     3721 cctgcaccac cgcctgctcg ccgtcgcgtc cgatgacctc gccgaccagt ccgagcttgc
     3781 cgatgcgcac ctgctcgccg ttcaacaccc gcggcagact cacggtgacg atcggtccgt
     3841 tgatttcgac aatttttccc gccgcgcgct tatccaccga tgattgctcc tatggctgcg
     3901 gttgaggcga acagacgctc catcactacc tgatgcaacg cctcctgcaa gcgctcgagg
     3961 cgcccctcga atgtgttatc cacgcggatg ctgtcggcgg cgtt
//
LOCUS       DBNGZE010000070         2459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_704396, whole genome shotgun sequence.
ACCESSION   DBNGZE010000070 DBNGZE010000000
VERSION     DBNGZE010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_704396"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1095)
                     /gene="ilvC"
                     /locus_tag="AC16FV_01740"
     CDS             complement(79..1095)
                     /gene="ilvC"
                     /locus_tag="AC16FV_01740"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR2848815.1"
                     /translation="MKVFYDKDADLSLIRGKKVAIVGYGSQGHAHALNLKESGVDVRV
                     ALREGSNSVQKAKNAGLTVKNIPDAVKEADLVMILAPDEQHKKLYQESVEPNLKKGAT
                     LAVAHGFSIHFGFIQPRADLDVIMIAPKGPGHLVRSTFTQGGGVPCLIAVKQNASGKA
                     REVALSYASAIGGGRAGVIETTFKDECETDLFGEQAVLCGGATHLVQAGYETLVEAGY
                     PPEMAYFECLHELKLIVDLMYEGGIANMRYSISNTAEYGDYTRGPRVVNEQTKAEMKK
                     ILKEIQSGQFAKEWMAENETGGKRFPEMRAQAAKHPIEEVGARLREMMPWIKAHRIVD
                     KTKN"
     gene            complement(1233..1724)
                     /gene="ilvN"
                     /locus_tag="AC16FV_01745"
     CDS             complement(1233..1724)
                     /gene="ilvN"
                     /locus_tag="AC16FV_01745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR2848816.1"
                     /translation="MRHTISMLLENESGALSRVAGLFSARGYNIESLSVAPTEDETLS
                     RLTLVTSGSEDVIEQITKQLNKLVDVVKLVDLTEGQHIEREMLLVKVKAGGATRNKIG
                     QLVKDYQGRIIDETETTYTIELTDTSSRLDAFVKAVDHEGIIEVVRSGVSGIARGEKS
                     LRV"
     gene            complement(1767..>2459)
                     /locus_tag="AC16FV_01750"
     CDS             complement(1767..>2459)
                     /locus_tag="AC16FV_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR2848817.1"
                     /translation="WKQIDQWRAMNCLDYDHDSKLIKPQYVLEKLYELTKGDAYITSD
                     VGQHQMWAAQFYRFDKPRRWINSGGLGTMGFGLPAAMGVKMAFPKETVCCVTGEASIQ
                     MCIQELSTCKQYDLPIKIVNLNNRYMGMVRQWQEFFYDRRYSHSYMDALPDFVKLTEA
                     YGHVGLKIEKPGDVDGALQEALKMKDRLVFLDFITDQTENVYPMIAAGKGQNEMHLKP
                     CKAVVPTDRELA"
BASE COUNT          469 a          757 c          662 g          571 t
ORIGIN      
        1 caagacgcca agaacgccaa gtttttcctt gatatccaaa actcttcttt cttggcgacc
       61 ttggcggtta atgaatattt agttcttggt cttgtcgacg atccggtgag ccttgatcca
      121 cggcatcatc tcgcgcaacc tggcgccaac ctcttcgatc ggatgcttgg ccgcctgcgc
      181 gcgcatttcg gggaagcgct tgccgccggt ttcgttctcg gccatccact ccttggcgaa
      241 ctggccggac tggatttcct tgagaatctt tttcatctcg gccttggtct gctcgttgac
      301 gacgcgcggc ccgcgcgtgt agtcgccata ctcggcggtg ttggaaatcg agtagcgcat
      361 gttggcgata ccaccctcgt acatgagatc gacgatgagc ttcaattcat gcaggcactc
      421 gaaatacgcc atctccggcg gatagcctgc ctccaccagt gtttcgtatc cggcctgcac
      481 cagatgcgta gcgccaccgc acagcaccgc ctgttcgccg aacagatcgg tctcgcactc
      541 atccttgaag gtggtctcga tgacgccggc gcgtccgcca ccaatggccg aggcatagga
      601 cagcgcgact tcgcgcgcct tgccgctggc attctgcttg actgcgatca ggcacggcac
      661 gccgccgccc tgggtgaagg ttgaacgcac cagatgcccc ggacccttgg gcgcgatcat
      721 gatgacatcc agatcggcgc gcggctggat gaaaccgaag tggatggaaa aaccgtgggc
      781 cacggccagc gttgcgccct tcttcaggtt cggctccacg ctttcctgat acagcttctt
      841 gtgctgctcg tccggcgcca ggatcatgac cagatcggct tctttcaccg cgtcgggaat
      901 gtttttgacg gtcagacccg cattcttcgc cttctgcacc gaattcgatc cctcgcgcag
      961 cgccacgcgc acgtccacgc cgctctcctt caggttcaag gcatgcgcat gaccctggga
     1021 gccgtaaccc acgatggcga ctttcttgcc gcggatgagg gaaaggtcgg cgtctttgtc
     1081 gtagaaaact ttcatgtgaa ctcctgtaat aaaatagaac gcagataaac gcagatttta
     1141 attatgatga gccggattga atggaattaa ccaaaataca tcagtgttta tctttaaaaa
     1201 gatctgcgtg catctgcgtt tgaaaataat atttacaccc gcaggctctt ctcgccgcgg
     1261 gcgatgccgg aaacgccgga acgcaccacc tcgatgatgc cctcgtggtc caccgccttg
     1321 acgaaggcat ccagccggct gctggtatcg gtcagctcga tggtgtaggt ggtttcggtt
     1381 tcatcgatga tccgaccctg atagtccttc accagctggc cgatcttgtt gcgcgtcgcg
     1441 ccgccggcct tgaccttgac cagcagcatt tcacgctcga tatgctggcc ttccgtgagg
     1501 tccaccagct tgaccacgtc caccagcttg ttgagctgct tggtgatctg ctcgatgaca
     1561 tcttccgagc cgctggtcac gagcgtgagg cgcgacaggg tttcatcttc cgtcggcgcc
     1621 acgctgagcg attcgatatt gtaaccgcgc gcggaaaaca accctgccac acgcgacagc
     1681 gcgcccgatt cgttttccag caacatggat atagtatgtc gcattaatgt ctccctgctc
     1741 cgcttcgctc cgttcgtccg accatattaa gcaagctccc gatccgtcgg caccaccgcc
     1801 ttgcagggct tcaaatgcat ttcgttctga cccttgccag cggcaatcat gggatagacg
     1861 ttctcggtct ggtcggtgat gaagtccagg aacaccaggc gatccttcat cttgagggct
     1921 tcctggagcg cgccgtccac gtcgccgggc ttctcgatct tgagtcccac atgcccgtag
     1981 gcctcagtca gcttcacgaa atccggcagc gcgtccatat acgaatgcga gtagcgccgg
     2041 tcataaaaga attcctgcca ctggcgcacc atgcccatgt agcggttgtt gaggttcacg
     2101 atcttgatcg gcaggtcgta ctgcttgcag gtcgagagct cctggatgca catctgaatc
     2161 gacgcctcgc cggtcacgca gcacaccgtc tccttgggga acgccatctt tacgcccatc
     2221 gccgccggca ggccgaaccc catcgtgccg agaccgccgg agttgatcca gcgccgcggc
     2281 ttgtcgaaac ggtagaactg cgccgcccac atctgatgct ggcccacatc ggaagtgata
     2341 taggcgtcgc ccttggtgag ctcgtaaagc ttctcaagca cgtactgcgg cttgatcagt
     2401 ttgctgtcgt ggtcatagtc gaggcagttc atggcgcgcc attggtcgat ctgtttcca
//
LOCUS       DBNGZE010000071         2530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1329859, whole genome shotgun sequence.
ACCESSION   DBNGZE010000071 DBNGZE010000000
VERSION     DBNGZE010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1329859"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16FV_01755"
     CDS             complement(<1..303)
                     /locus_tag="AC16FV_01755"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR2848818.1"
                     /translation="MRVITLNVNGIRSAAKKGLFEWLPRQQADVVCLQEVKAQEPDLS
                     EPMRAPKGFHGFFNCAEKKGYAGVALYTRRAPDKVRVGFGSKEHDDEGRYLQADFGK"
     gene            359..1000
                     /gene="pyrE"
                     /locus_tag="AC16FV_01760"
     CDS             359..1000
                     /gene="pyrE"
                     /locus_tag="AC16FV_01760"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR2848819.1"
                     /translation="MKDYQREFLDFAIECQALRFGEFTLKSGRISPYFFNSGLFNTGR
                     KLARLGRFYAQAIADSGIAFDMLYGPAYKGVPLACATAMAIAENGERDMPYAFNRKEV
                     KKYGEGGNIIGHPLQGKVLIVDDVISAGTSVRESVQIIKTANATPAGVTIALDRQERG
                     EGPVSAVQEVETTLGLRVISIVKLETLLKYLDGKDGGRRYLDAITAYRRAYGT"
     gene            1035..1577
                     /locus_tag="AC16FV_01765"
     CDS             1035..1577
                     /locus_tag="AC16FV_01765"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2848820.1"
                     /translation="MIAVSRNIFIEAPVERVFALMADPAARSALSPHAKPIQVEIEGG
                     ESLRVGSVCHFRLQMDNRIVDYHTRVCEFMPNRLIVSISDTAVPFRIRLETRPEGDGT
                     RLAHTEQFEPTEEMLRQALPPTLTNTVMEKIYWLLPFLDPDYAARVQRGREDNLTLKL
                     GANLEQWLAAIKQHLESSRD"
     gene            complement(1581..2021)
                     /locus_tag="AC16FV_01770"
     CDS             complement(1581..2021)
                     /locus_tag="AC16FV_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR2848821.1"
                     /translation="MKIEEILKSFGRKQRRRTLDHVEDFTTMLGPDSVYTGVFQGKDN
                     HIIYGEVQGECELEGILVLGEGSRWKGNIRAGSVVIAGRVDGDIEAKDKLELARTAYV
                     RGKITSPVVAIARGAVHEGSIRMTKQTQITRFVERREAPDKDRD"
     gene            complement(2101..>2530)
                     /locus_tag="AC16FV_01775"
     CDS             complement(2101..>2530)
                     /locus_tag="AC16FV_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2848822.1"
                     /translation="TIGLGLYSFFNKQLGLTHAPIHRTRHGYALGGLGLFVVGVLNGS
                     LTSGTGLFCTMWLVRWFGLDYRRAVAYTLVLVGLFWNGAGAVTLGILGEIKWSWLPAL
                     LVGSLLGGYLGAHWSIIKGNLWIKRTFEIITLLVGLKLIF"
BASE COUNT          510 a          820 c          751 g          449 t
ORIGIN      
        1 cttgccgaaa tcggcctgaa gataacgccc ttcgtcatcg tgctccttcg agccaaagcc
       61 gacacgcacc ttgtccggcg cgcgccgcgt atacagcgcc acgccggcgt agcccttctt
      121 ctcggcacaa ttgaaaaaac cgtgaaatcc tttaggtgcg cgcatcggtt cggagaggtc
      181 gggctcctgc gccttgacct cctgcagaca cacaacatcc gcctgctggc gcggcagcca
      241 ttcgaatagt ccctttttgg ccgcggaacg gatgccgttg acgttgagcg tgatgacgcg
      301 catgacgccg gatgataccc gactttcccg cgcctgaggc ggcgattaca atccgcgcat
      361 gaaagactac cagcgcgaat tcctcgactt cgccatcgag tgtcaggccc tgcgcttcgg
      421 cgagttcacc ctgaaatcag gacgaatcag tccctatttt ttcaacagcg gcctgttcaa
      481 caccggccgc aagctggcgc ggctggggcg tttctacgca caggccatcg ccgacagcgg
      541 catcgccttc gacatgctct acggccccgc ctacaagggc gtgccgctgg cctgcgccac
      601 ggccatggcc atcgccgaaa acggcgaacg cgacatgccg tatgccttca accgcaagga
      661 agtgaaaaaa tatggcgagg gcggcaacat catcggccat ccgttgcagg gcaaggtgct
      721 gatcgtggac gacgtgatct cggccggcac ctccgtacgc gaatcggtgc agatcatcaa
      781 aaccgccaat gccacgccgg cgggcgtgac catcgcgctc gaccggcagg aacgcggcga
      841 ggggccggtg tccgcggtgc aggaagtgga gaccaccctg ggactgcgcg tgatcagcat
      901 cgtcaagctt gagacgttgc tgaaatacct ggacggaaaa gacggcggac gacgctacct
      961 cgacgcgatc accgcttacc gccgcgccta cggcacctga acacctgacc actgtctgaa
     1021 acggggggat caggatgatc gccgtttccc gtaatatctt catcgaggca ccggtcgagc
     1081 gcgtgttcgc gctcatggcc gacccggcgg cgcgcagcgc gctctcgcct cacgcgaaac
     1141 ccattcaggt ggagatcgaa ggcggcgaat ccctgcgcgt cggcagcgtg tgtcacttcc
     1201 ggctgcaaat ggacaatcgt atcgtcgatt atcacacccg cgtgtgcgag ttcatgccga
     1261 accgcctgat tgtttccatc tccgacaccg ccgtgccatt tcggattcgc ctcgaaaccc
     1321 ggccggaggg cgatggcacg cgactggccc ataccgaaca gttcgaaccg accgaggaga
     1381 tgttgcgcca ggccttgccg cccacgctga cgaacacggt gatggagaaa atatactggc
     1441 tgctgccgtt tctcgatccg gactatgccg cgcgcgtgca gcgcgggcgg gaggacaacc
     1501 tgacgctgaa actcggcgcg aacctggagc agtggctcgc ggccatcaag caacatctcg
     1561 aatccagccg cgactgaggg ctaatccctg tctttgtccg gcgcctcgcg gcgctcgaca
     1621 aaccgcgtga tctgggtctg cttggtcatg cggatactgc cctcgtgcac cgcgccgcgg
     1681 gcgatagcca ccacgggact ggtgatcttg ccgcgcacat aggcggtgcg cgccagttcc
     1741 agtttgtctt ttgcctcaat gtcgccgtcc acccgtcccg cgatcacaac gctgccggcg
     1801 cggatgtttc ccttccagcg actgccctcg cccagcacga gaatgccctc gagctcgcat
     1861 tcgccctgca cctcgccata aataatatgg ttgtccttgc cctggaacac gccggtgtac
     1921 accgaatccg gcccgagcat ggtggtgaaa tcctcgacgt ggtcgagggt gcggcggcgc
     1981 tgtttgcggc cgaacgactt caggatctct tcgattttca aggctgttcc cggtagtgtg
     2041 ttttgtcggg tgagttgtgg acctctccaa atactagcag gcaaaaaata cgtcgcgagg
     2101 tcagaagatg agcttgagcc ccaccagcag ggtgatgatc tcgaaggtgc gcttgatcca
     2161 gagattgccc ttgataatgg accaatgggc gccgagatac ccgcccagaa gcgagccgac
     2221 cagcagcgcc ggcagccatg accacttgat ctcgcccaga atgcccaggg tgacggcgcc
     2281 agcgccgttc cagaacagcc cgaccagcac cagcgtgtag gccaccgccc ggcgataatc
     2341 gagaccgaac cagcgcacca gccacatcgt gcagaacaga cccgtgccgg aggtgagcga
     2401 gccgttgagc acgccaacca cgaacaggcc gagcccgccg agggcatagc catgccgcgt
     2461 gcgatggatg ggcgcatgcg tcagcccgag ctgcttgttg aagaacgaat aaagcccgag
     2521 gccgatcgtg
//
LOCUS       DBNGZE010000072         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1399442, whole genome shotgun sequence.
ACCESSION   DBNGZE010000072 DBNGZE010000000
VERSION     DBNGZE010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1399442"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..951)
                     /locus_tag="AC16FV_01780"
     CDS             complement(229..951)
                     /locus_tag="AC16FV_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848823.1"
                     /translation="MATSALSLSHIDAFYGDSHVLGDVSFELGEARMLGLLGRNGAGK
                     STCMNVTMGLLPPRRGEVAVFGQQVSGLSPEMIAARGVALVPQGRRIFKSLTVRENLV
                     VAARKPDAGSKHAPWTTDTVFGMFPRLAERGNQIAAHLSGGEQQMLAIGRALMGNPRV
                     LLMDEPSEGLAPQIVAEVMATIRKLKESGLSIVLVEQNPKLVFDVADDIVILNTGRVA
                     VVSTPAQLEKDGVDLRQHLGVF"
     gene            complement(941..1678)
                     /locus_tag="AC16FV_01785"
     CDS             complement(941..1678)
                     /locus_tag="AC16FV_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848824.1"
                     /translation="MSALTVTDLCKSFVGLHVTNDVNLNVEPGERRLIIGPNGAGKTT
                     LFNLITGELTPDSGSIHLFGQDITQVPSRRRAHLGMARTYQIITLFARDTIIHNVTLA
                     LLGLSPARWNPLIDLKRQGHFVERAHAALERVGLAHIANRPLAQTSYGERRRVEIAMA
                     LTQNPKVLLLDEPFAGLSIDERRDVKQLLLAIPRDVTIVMIEHNMDVALEFAERITLL
                     HFGEVIVEGNRAEVVADPRTREVYLGH"
     gene            complement(1675..>2481)
                     /locus_tag="AC16FV_01790"
     CDS             complement(1675..>2481)
                     /locus_tag="AC16FV_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2848825.1"
                     /translation="GLFGVTAYAAGYMLQLGFGHPVAILVALVIGVASMAIYAALSLR
                     STGIGFIMITLALGEILWGLAYRWISLTGGDNGLSVKTRPEPFGYSLSDANTFYYATL
                     VVFLLSLAAMTVFVRSPLGAALMGTRDQPRRMNALGYHVWAIRFWACLISGLLTAVSA
                     ILFVYYTQFISPQTLALTSSAEVLLMVISGGAGTLLGPIVGSGLVVVVKTVVSGFIER
                     WNFLLGAIFVAIVILMPEGLVPGTVRLWRLAWRSREDSGGGIKGSAEAKS"
BASE COUNT          504 a          763 c          760 g          454 t
ORIGIN      
        1 cgtcgagatg tggcttgatg aactccgcgg cgcgcggaac ggcaatatgc gtgtgggcgt
       61 cgatggtcgt gctctttggc cgcacgtcgc gtccaggctc gccacgcttg cgcgcggcgg
      121 tgtccgcata tttattccag atgtcagcca tttgcagcct ctcgtttgtc atccgcaaat
      181 acggatgatc cagtagtctc agcgcttccg aatttcaacg cgaaggactc aaaaaactcc
      241 aaggtgttgg cgcaaatcga cgccgtcctt ttccaattgc gctggcgtcg aaaccactgc
      301 gacgcggccg gtattgagaa taacgatgtc gtcagcgacg tcgaatacca gcttgggatt
      361 ctgttcgacc aatacgatcg acagcccact ttccttcagc ttgcggatgg tggccatcac
      421 ctcggcaaca atctgtggcg ccaacccttc cgagggttca tccatcaaca acacgcgggg
      481 atttcccatc agcgcgcgac caatggccag catttgctgt tcgccacccg ataggtgcgc
      541 tgcgatctga ttgcctcgtt cggccaggcg cggaaacatc ccgaacacgg tgtccgtggt
      601 ccagggcgcg tgcttgctgc cggcatcggg tttgcgggcg gcgacgacca gattctcacg
      661 gactgtcagg ctcttgaaga tgcggcggcc ctgcggcacc agcgcgacgc cccgggccgc
      721 gatcatttcc ggcgacaggc cgctcacctg ttgcccgaag acggccacct cgcctcgccg
      781 cgggggcagc aggcccatgg tgacattcat gcaagtcgat ttaccggccc cgttgcggcc
      841 gagcagaccg agcatgcgtg cttcgccgag ttcgaacgac acatcgccga ggacatggct
      901 gtcaccataa aacgcgtcga tgtggctaag gctgagcgcg ctagtggcca aggtagacct
      961 cccgcgtgcg cggatccgca accacttcgg cacggttgcc ctcgacgatc acctcgccga
     1021 aatgcaggag cgtgatgcgc tcggcgaatt ccagtgccac gtccatgttg tgctcgatca
     1081 tcacgatggt gacgtcgcgc gggatagcca gcagcagttg ctttacgtca cggcgctcgt
     1141 cgatcgacaa gccagcgaac ggctcgtcga gcagcagcac tttcggattc tgcgtcaacg
     1201 ccatcgcaat ctcgacgcgg cggcgttcgc cataggatgt ctgcgccaac ggccgattcg
     1261 cgatatgtgc gagaccaacc cgttccagcg ccgcgtgcgc acgttcgaca aagtgaccct
     1321 gccgtttaag atcgatcagc gggttccagc gtgccggtga caagccgagc agcgccagcg
     1381 ttacattgtg gatgatggtg tcgcgcgcga acaacgtgat gatctgatag gtgcgcgcca
     1441 tgccgagatg ggcacggcgg cggcttggca cctgagtgat gtcttgtccg aagagatgga
     1501 ttgagccgga gtcgggggtc agctctcccg tgatcagatt gaacagggtg gtcttgccag
     1561 cgccgttcgg gccaatgatc agccgccgct cgcccggctc aacattgaga ttgacgtcgt
     1621 tggtcacgtg caggccgacg aacgatttgc agaggtccgt gacggtgagc gcgctcatga
     1681 cttcgcctct gcgctgccct tgatcccgcc cccgctgtct tcgcggctgc gccacgcaag
     1741 cctccacagc cgtaccgtgc caggaaccaa gccttcaggc atcaagatga cgatggcgac
     1801 gaagatggca ccgagcagaa aattccagcg ctcgatgaag ccgctcacca cggtcttgac
     1861 caccaccacg agtccagagc ccacgatcgg cccgagcagc gtgcctgcac cgccggagat
     1921 caccataagt agcacctcgg ccgaggacgt gagggctaat gtctgcggac tgataaattg
     1981 cgtgtaatag acgaacagga tggcggacac cgcggtcagg agcccggaga tcaagcaggc
     2041 ccagaagcgg atcgcccaaa cgtgatatcc gagcgcgttc atgcggcgcg gctggtcgcg
     2101 ggtgcccatg agtgcggcac cgagcggtga gcgtacaaat accgtcatgg ctgcgagcga
     2161 caacaggaag acgaccaacg tcgcgtagta gaacgtattg gcatccgaca gcgaatagcc
     2221 gaacggctcg gggcgggtct ttacgctcag tccgttatcg ccgccggtca ggctgatcca
     2281 gcgataggcg aggccccaga gaatctcgcc gagtgccagc gtgatcataa tgaagccgat
     2341 gccggttgag cgtagcgaga gcgctgcgta gatcgccatc gaagccacgc cgattaccag
     2401 cgcgactaga attgccaccg gatggccgaa gccgagttgc aacatgtaac cggccgcgta
     2461 agcggtcacg ccaaaaaggc c
//
LOCUS       DBNGZE010000073        10567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_704584, whole genome shotgun sequence.
ACCESSION   DBNGZE010000073 DBNGZE010000000
VERSION     DBNGZE010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_704584"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="AC16FV_01795"
     CDS             <1..861
                     /locus_tag="AC16FV_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HIR2848826.1"
                     /translation="CSTIIESGIDVPSANTIIIERADKFGLAQLHQLRGRVGRSHHRA
                     YAYLLVPDRRALTEDARKRLEAIESLEELGAGFTLASHDLEIRGAGELLGETQSGMID
                     EVGFTLYSELLNRAVRSLQAGRLPETDTSLHSGTEINLHAPALFPESYLPDVHMRLVL
                     YKRLSTLKDLGALQELKEEIIDRFGVLPEPGQLLFRATEIKIRATPLGIRKIDAGPRG
                     ARIEFIEKTDVDPGSILRLLQSAPRRYRLDGPNRLRILGDMPDAAARITAVSRFLESL
                     VPETQTAGTG"
     gene            complement(863..1381)
                     /gene="def"
                     /locus_tag="AC16FV_01800"
     CDS             complement(863..1381)
                     /gene="def"
                     /locus_tag="AC16FV_01800"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR2848827.1"
                     /translation="MTVRRVLKMGDPLLYRKAEPVQQFNTPELDKLIADMFDTMAELS
                     GAGLAAPQIGVSQRVVIFGVEANPRYPHVEPVPTTVLVNPVVTPIGHDMEDGWEGCLS
                     VPGLRGLVSRHLNLRYTGFDQHGNPIDRTVSGFHARVVQHECDHLDGILYPMRLRDIR
                     LLGFEDALFPDL"
     gene            1448..2035
                     /locus_tag="AC16FV_01805"
     CDS             1448..2035
                     /locus_tag="AC16FV_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HIR2848828.1"
                     /translation="MKGLLLAIFLIAAPVAALHAAPVATNMYDVEVVVFENRLSDLEN
                     GELWARDPEKSAAAGKDESVSAGENPPADSALSAAAAALEKSGRHHVLARLRWQQSAE
                     AKSVSKPVKISNTAAGLDGSLRFYLSRFLILELNLALREAQSGGMLSGAAEKGAAVYR
                     LNEPRRIKVSETHYFDHPKFGALVRVSPAKAGGQR"
     gene            complement(2026..2778)
                     /locus_tag="AC16FV_01810"
     CDS             complement(2026..2778)
                     /locus_tag="AC16FV_01810"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR2848829.1"
                     /translation="MTAVAIIGGSGLTNLKNLRITRREVIRTPYGEPSAPMVFGQLGG
                     QEAVFLPRHGAGHTIPPHEVNYRANIWAIKQTGVDKVIAVNAVGGISSDYLASGTLVI
                     PDQVIDYTYGRAHTFFGSEHKKVTHVDFTYPYCEELRQTVIQAAARAKMKVLGRGTYG
                     ATQGPRFESAAEIRRMERDGADIVGMTGMPEAGLARELELCYVSIALVVNPAAGKAKD
                     TISIKEIEKNLETGMASVRSLLEQVIPLIVQR"
     gene            complement(2823..3407)
                     /locus_tag="AC16FV_01815"
     CDS             complement(2823..3407)
                     /locus_tag="AC16FV_01815"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIR2848830.1"
                     /translation="MNPISAQQAWEVLQRADCLHSPERVEAALADMATRINQTLAGTD
                     PLVVCVMTGGVVPFGQLLPRFRFPLQIDYVHATRYGKKLHGGQLQWISGPHQDPRGRV
                     VLLIDDILDEGTTLAGIEARYRAEGARELYKAVLVVKNRPRQHDVKVEFAGLEVPDRY
                     VFGCGMDYKGYLRNIAGIYAEAEARLGKTNERSE"
     gene            complement(3433..3801)
                     /locus_tag="AC16FV_01820"
     CDS             complement(3433..3801)
                     /locus_tag="AC16FV_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR2848831.1"
                     /translation="MADKLMIVMVNTDPSDPSELGAPFFQATVAAAMEYEVEVILTAR
                     AGELAIKGVAEKLRVQEGSPKSVYDFIKDAHEAGVKFKVCTPTLELWGNELIPEIEET
                     VGGAYVISQAMDEDVVTFTY"
     gene            complement(3945..4814)
                     /gene="galU"
                     /locus_tag="AC16FV_01825"
     CDS             complement(3945..4814)
                     /gene="galU"
                     /locus_tag="AC16FV_01825"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR2848832.1"
                     /translation="MKKVTKAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYAAEE
                     AIAAGIDELIFVTGRNKRSIEDHFDKAYELEAELEADGKHKLLATVQNVLPGKASCVY
                     IRQTEALGLGHAVLCAKQVVGDDPFAVILADDLISSEPSALRQMADLYESCQCSVIGV
                     QNISRDETQSYGIVKAKALGPRIHTVEGIVEKPSPEKAPSTLAVVGRYILTPRIFGLL
                     ENVTPGAGGEIQLTDGIAALLSHEKVMAYEFIGKRYDCGSKLGYLQATVEYALEHSEL
                     KEKFRAYLKGLNF"
     gene            complement(4846..6177)
                     /locus_tag="AC16FV_01830"
     CDS             complement(4846..6177)
                     /locus_tag="AC16FV_01830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR2848833.1"
                     /translation="MKLTIIGTGYVGLVTGACFAEMGNTVTCVDIDEMKVDGLKKGIL
                     PIYEPGLESVVINNYKNGRLQFCTSLSDPAAESGVYFIAVGTPPGEDGSADLQYVLAV
                     AREIGRHINSYTIVVDKSTVPVGTADKVNAAIRDELTKRGVEVAYDVVSNPEFLKEGA
                     AVEDFMRPDRVIIGTDSEQVRKIMRDLYLPFMRAHERLLFMGVRDAEMSKYAGNAMLA
                     TKISFMNEIANLCERLGVDVENVRQGIGSDSRIGYSFIYPGAGYGGSCFPKDVKALIR
                     MAKDQKFEPKVLNAVEARNEAQKHVLFEKITRRFGAKLKGLTFGLWGLSFKPGTDDMR
                     EASSVVLLHELIGAGATVKAYDPVAMTAARRELPGEWFKTDQLKLVDEQYSALDGADA
                     MVLMTEWKPFRHPDFGRIRAVMKHPVIFDGRNQYDPALLREAGFEYSGIGR"
     gene            complement(6226..8097)
                     /locus_tag="AC16FV_01835"
     CDS             complement(6226..8097)
                     /locus_tag="AC16FV_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR2848834.1"
                     /translation="MVPLAWFGAYWLRFNLDSIPEGFFNQALMLLPIIVVAQGGMFWY
                     FGLYRGIWRFASIPDLVRILKAVTAGVVVAAAASFILTRLQSVPRSVFVLDGFLLVLL
                     LGGPRFIYRLFKDHSLYQWIQDRSLTANAERKNALIVGAGKAGETLARDLLRDPSGAY
                     LPVAFADDDRGKAGKEIHGIPVVGSCYQIPQIVTKHGIDLIIIALPAATSRQIRRIVE
                     ICETTGLPFRILPQMQDLVSGKASLKDLRDVRIEDLLGREPVELDWQAITEATRGKTV
                     LVTGGGGSIGAELCRQLARLKPSQLIILDRSEFNLYSIDIELRQGMPELSLVSLLVDI
                     NDVVQMEKILRAYHPSVIYHAAAYKHVPILEDQARAAVVNNVFGTRVVANLADKFGCE
                     SFVMVSTDKAVNPANVMGASKRVAEMYCQGMNAKSKTRFITVRFGNVLGSSGSVIPLF
                     QQQIAQGGPVTVTHPDIQRYFMTIPEASQLILQAGVIGKGGEIYVLDMGEPVKISYLA
                     EQLIRLSGKTPGEDVEIVYTGLRPGEKLYEELFHDAEKLAETSHPKILLAQCRMMDKD
                     ILENSFDAMQQACEKGDNSALRGMLAELVPEHTGLVASPSVTEKSAVVVSLKQTKSP"
     gene            complement(8299..9327)
                     /locus_tag="AC16FV_01840"
     CDS             complement(8299..9327)
                     /locus_tag="AC16FV_01840"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR2848835.1"
                     /translation="MPMLIFLVPAIAALGLSAWLSWRFCNPSSLFHILDNPNERSLHS
                     NPTPRSGGIAILAGLLAAGALIWILQGVALYMWFLAALVPVAIVSYLDDRAGVAVLLR
                     LLAHVVGAGLLVWSGSFSSENGLLPGMELPWFGLLVILAYVVWMINLYNFMDGMDGFA
                     AGMAVIGFGFLAITGLLTENLQFAQTCLVVTGGAAGFLFFNFPPARIFMGDVGSSSLG
                     LLAAAFSLWGARDGVFPFWVAILIFSPFIADASVTLVKRMLCGEKIWQAHKTHYYQKL
                     VQAGLGHRKTVLIEYAIMIGCGITALLSLRATVVIQAVMLAGWVLFYIIFFTWVSWYA
                     SGRHHDSV"
     gene            complement(9346..10326)
                     /locus_tag="AC16FV_01845"
     CDS             complement(9346..10326)
                     /locus_tag="AC16FV_01845"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase family protein"
                     /protein_id="HIR2848836.1"
                     /translation="MTFVTGANGFVGKALIAELLHRGVEVRAAVRRESDIILSNSSEP
                     LSVHIVGDIGPKTDWGTSLDGIQAVVHLAARVHVMKEQALDPLMEFRRVNTLGTLRYA
                     SMAAQAGVRRFVYVSTIKVNGEATTSMPFREDDRPRPSDPYSVSKWEAEQGLRRIASE
                     TGMEIVIIRPPLVYGPGVGGNFLTILKWIDRGIPLPLASVRNQRSFVGLSNLVSLIIR
                     CLSHPDAAGEVFLVSDGEDLSTPDLFQRVAHALGKRSRLFPLPVSLLRTMARVLGKDS
                     VCERLCGSLKVDSGKVRQLLGWVPPLSVDGELQATGEWYRRNISKDIALL"
BASE COUNT         2484 a         3130 c         2712 g         2241 t
ORIGIN      
        1 tgctcgacga tcatcgaatc cggcatcgac gtgcccagcg ccaataccat catcatcgaa
       61 cgggcggaca agttcggcct ggcacagctg caccagctgc gcggtcgcgt cggacgctcg
      121 caccatcgcg cctacgccta cctgctggta ccggaccggc gcgcgctaac cgaagacgcg
      181 cgcaagcgcc tcgaggccat cgagtcgctg gaggaactgg gagcggggtt tacgctcgcc
      241 tcgcacgatc tggaaatacg cggcgcgggc gagctgctgg gggagaccca gagcggaatg
      301 attgacgagg tggggtttac gctttattcg gaactgctga accgggcggt gagatcactg
      361 caggccggca gactgccgga aacggataca tcattgcaca gtggaaccga aatcaatctg
      421 catgcaccgg cgcttttccc cgagagctac ctgccggacg tgcacatgcg gctggtgctc
      481 tacaaacgcc tgtctacctt gaaggatctt ggcgcacttc aggagctgaa agaggaaatc
      541 atcgaccgtt tcggggtgct gccggagccc ggccagctgc tgtttcgcgc cactgagatc
      601 aagatacgtg ccacgccgct gggcatccgc aagatcgatg ccggtccgcg tggcgcgcgc
      661 atcgagttca tcgaaaaaac ggacgtcgat cccgggtcga ttctgcgcct gctccagtcc
      721 gcgccgcgcc ggtaccggct cgacggcccc aaccgcttac gcatactggg agacatgccg
      781 gatgccgcgg cacgcatcac ggccgtgagc cgttttctcg aatcactggt tcccgagacg
      841 cagacggccg gcacgggatg actcaaaggt cgggaaacag ggcgtcttca aaacccagca
      901 ggcgaatgtc gcgcagccgc atgggataca aaataccgtc gaggtgatcg cattcgtgtt
      961 gcaccacgcg cgcgtgaaaa ccggaaaccg tgcgatcgat cgggttccca tgctgatcga
     1021 agccggtgta acgcagatta agatgccggc tgaccaggcc gcgcaggccc gggacactga
     1081 gacagccctc ccagccgtcc tccatgtcat ggccgatggg tgtcaccacc ggatttacca
     1141 gaacggttgt cggcaccggt tccacgtggg ggtaacgcgg gttggcctcg acgccaaaaa
     1201 tcaccacacg ctgcgagacg ccgatctgtg gcgccgcgag acccgcgcca ctcaactcgg
     1261 ccatggtgtc aaacatgtcg gcaatcagtt tatcgagttc aggcgtatta aattgttgca
     1321 ccggctccgc tttccggtac aacaacggat cgcccatctt gagtacgcgc ctgaccgtca
     1381 tttcacacgc tcgaaattgt gcggggaata agggatacta taaataattc aacgcaagga
     1441 aaacaccgtg aaaggactgt tgctagcgat ttttctgatt gctgcgccgg ttgcggcgct
     1501 tcatgcggcg ccggttgcta ccaatatgta tgatgtcgag gtggtcgtgt tcgagaaccg
     1561 gctctccgat ctcgaaaacg gcgagctttg ggcgcgcgat ccggaaaaat ccgcagccgc
     1621 cggaaaggat gaatcggtga gcgccggtga aaatcctccc gcggattcgg ccttgtcagc
     1681 cgcggcagcg gcgctggaaa aatccggccg gcatcatgtg ctggcccgtc tacgctggca
     1741 acagagcgcc gaagccaaat ccgtgtcaaa accggtcaag atcagcaata cggcggcggg
     1801 gctggatgga tcgctgcggt tttatctgag ccgcttcctg attctggaac tgaatctcgc
     1861 cctgcgtgaa gcgcaaagcg gaggcatgct cagcggtgcg gcggaaaaag gcgctgcggt
     1921 gtaccgtctg aacgagcccc ggcgcatcaa ggtatcggaa acccattatt tcgaccaccc
     1981 gaaattcggt gcattggtgc gcgtttcacc cgccaaggcc ggcggtcagc gctgaacaat
     2041 cagggggatc acctgctcca ggagcgagcg caccgacgcc attcccgtct ccagattctt
     2101 ttcgatttcc ttgatgctga tggtgtcttt ggccttgccg gccgccggat tcaccaccaa
     2161 ggcaatcgag acgtagcaga gttccagttc gcgtgcgaga ccggcctcgg gcatgccggt
     2221 catgccgacg atatccgccc catcacgttc catgcgtcgt atttccgcgg cgctttcgaa
     2281 gcgcggaccc tgggttgcac cataggtacc acgccccaac accttcattt tggcgcgcgc
     2341 cgccgcttgt atcacggtct ggcgcagttc ctcgcaatac ggataggtga aatcgacgtg
     2401 ggtcaccttt ttgtgctcgc tcccgaaaaa cgtatgcgcg cgcccgtagg tgtaatcaat
     2461 gacctgatca ggaatcacca gcgtgcctga ggccagataa tcggacgaaa taccgcccac
     2521 cgcgttaacg gcaataacct tgtcgacacc ggtctgtttt atggcccaga tgttagcgcg
     2581 gtaattgacc tcatggggcg gaatcgtgtg tcccgcccca tgacggggta aaaacacggc
     2641 ttcctgtcca ccgagctgcc cgaaaaccat gggcgccgat ggctcgccgt aaggcgtgcg
     2701 aatgacctcg cggcgcgtga tgcgcagatt tttgagattg gtcagaccgc tgccgccaat
     2761 aattgccact gctgtcatta atgtagccct acttcgttcg tcctgatata agagtagtag
     2821 ccctactcgc tccgctcgtt cgtcttgcct aatctcgcct ccgcctcggc gtaaattccc
     2881 gcgatattgc ggaggtaacc cttgtagtcc atgccgcaac caaacacgta ccgatccggc
     2941 acctcgagcc cggcaaattc cactttcaca tcatgctggc gcggacggtt ctttaccacc
     3001 agcaccgcct tgtatagttc gcgcgcgccc tcggcccgat agcgagcctc gatacctgcc
     3061 agtgtcgtgc cctcatccaa aatgtcatcg atcagcaaaa ccacccggcc acgtgggtcc
     3121 tgatgcggac cggatatcca ttgcaattga ccgccgtgca attttttccc gtaacgggtc
     3181 gcatgaacat aatcaatctg cagtggaaac cgaaaccggg gcaaaagctg gccgaacggc
     3241 accacgccgc cggtcatgac gcagaccacg aggggatcgg tgccagcgag ggtctggttg
     3301 atgcgcgtgg ccatgtccgc gagcgccgct tccactctct ccggagaatg taaacagtcc
     3361 gcccgctgca gcacttccca tgcctgctgc gcgctgatcg ggttcaacgt tggatatttt
     3421 tattctggat gattaatagg tgaaggtaac aacgtcttca tccatcgcct gactgatgac
     3481 ataggcgccg cccacggtct cctcgatctc gggaatcagc tcattgcccc acagctcgag
     3541 ggtcggtgtg cataccttga atttcacacc ggcttcatgg gcatccttga tgaaatcata
     3601 aacggacttc gggctgcctt cctgcacgcg cagtttctcc gccacgccct tgatggcgag
     3661 ctcgccagcg cgcgcggtca ggatcacctc tacctcgtat tccatcgccg cggccaccgt
     3721 tgcctgaaaa aacggtgccc ccagttcgga cgggtccgaa gggtccgtgt tcaccatcac
     3781 gatcatcagt ttgtcagcca tgcgattctc ctgaatactg cgaaatgccg gtatcttgtt
     3841 tcaggcggat tatatctgcc ccttgcttcc caaggctacc gtaacagcca gaaccggtag
     3901 caaacagaac ctacaccagg acaaggatac agtcttgatc aatatcaaaa gttgaggcct
     3961 ttcagatagg cgcggaactt ctctttgagc tcggagtgtt ccagggcata ttcaacggtc
     4021 gcctgcaggt aacccaattt gctgccgcaa tcgtaacgtt ttccgatgaa ctcataagcc
     4081 atcacttttt cgtgggataa caatgcggca atgccatcgg ttaactggat ctcgccgcca
     4141 gcgcctggcg tgacgttctc aagcaaaccg aaaatccgtg gtgtcagaat ataccggccg
     4201 acaactgcca gagtggatgg ggccttctcc ggcgacggtt tttccacaat cccttccacg
     4261 gtatgaatac gcggccccag agctttcgcc tttacgattc cgtaactttg cgtctcgtcg
     4321 cgtgagatat tctgcactcc aataacggag cattggcatg actcatacaa atccgccatt
     4381 tgtcgtaggg cactcggctc gctgcttatg aggtcatcgg caagtatgac ggcaaacggg
     4441 tcgtcaccca ccacttgctt ggcgcacaac accgcatgtc cgagcccgag ggcctcggtc
     4501 tgacgaatat aaacgcagga agccttgccc ggcaggacat tttgcacagt ggccagcagt
     4561 ttatgtttgc catccgcttc cagttcggct tccagctcgt aggccttgtc aaaatggtcc
     4621 tcgatcgaac gtttgttgcg gcccgtgacg aatatcaact catcgatacc ggcagcgatc
     4681 gcctcttccg ccgcatattg aatcaatggt ttgtcgacaa tcggcagcat ttccttgggg
     4741 cttgccttcg tggccggcaa aaaccgcgtg cccaagccgg cgaccgggaa tacagccttg
     4801 gtaactttct tcattcttct gcctgtgaat atattctgtt aacggtcaac gtccgatgcc
     4861 gctgtactca aatccggctt cgcgcagcaa cgccggatca tattgattgc gaccatcgaa
     4921 tataacgggg tgcttcatga ctgccctgat gcggccgaaa tcaggatgac ggaagggttt
     4981 ccattccgtc ataagcacca tggcatcggc cccgtcgagc gcgctgtatt gttcatccac
     5041 cagtttcagt tgatctgtct taaaccattc gcccggaagt tcgcgtctgg cggccgtcat
     5101 ggccaccgga tcgtaagcct ttaccgtggc tccggcacca ataagttcgt gcaacaacac
     5161 taccgaggat gcctcgcgca tatcatccgt gccgggtttg aatgacaggc cccagagacc
     5221 gaacgtcagg cccttgagct tggcgccaaa acgcctggta atcttttcaa acagtacatg
     5281 tttctgggcc tcgtttcttg cctccacggc gttaagcacc ttgggctcaa atttctgatc
     5341 cttggccatg cgaatcagcg ctttgacgtc tttcggaaaa caggaaccgc cataaccggc
     5401 tcctggataa ataaacgaat acccaatccg cgaatcagag ccaattccct ggcgaacgtt
     5461 ctcaacatcg acgcccagac gttcgcacag attggctatc tcattcatga atgagatctt
     5521 ggtggcaagc atggcattac cggcatactt gctcatctcg gcatcccgca cgcccatgaa
     5581 cagcaggcgc tcatgagcgc gcatgaacgg aagataaaga tcgcgcatga tcttgcggac
     5641 ctgctcgctg tcggtaccga taatgacgcg atccggacgc atgaaatctt cgaccgccgc
     5701 gccttccttc aaaaattcgg gattactgac aacatcataa gcaacctcga cgcctcgctt
     5761 ggttaattca tcgcggatag cggcgttgac tttatccgcc gtacccaccg gaacagtcga
     5821 tttgtccacg acaatggtgt aactattgat atgccggcca atctcgcgag ccaccgccag
     5881 aacatattgc aggtccgccg aaccatcctc gccgggcgga gtgcccacgg cgatgaaata
     5941 cacaccggat tcggcggcgg ggtccgacag tgacgtacag aattgcagtc ggccattctt
     6001 gtaattgttt atgacgacgg actccaggcc cggttcgtat atcggcaaaa tgcctttctt
     6061 gagaccgtcg accttcattt catcgatatc aacacaggta acggtgttac ccatttccgc
     6121 aaaacacgcg cccgtcacaa ggccgacata gcccgtaccg ataatcgtca acttcatgga
     6181 tggtatacct gcaaggaata attaattatg ttgttttaag ctctctcagg gagattttgt
     6241 ctgcttcaat gaaactacca cggcactttt ttccgtgacg gatggcgaag ccaccagacc
     6301 ggtatgctcg ggaaccagct ccgccaacat acccctcagt gcgctgttat ctcctttctc
     6361 gcaagcctgc tgcatggcat cgaaagaatt ctcaagtata tccttgtcca tcatgcggca
     6421 ctgcgccagc agaattttgg ggtggctggt ttccgccagt ttctccgcgt catggaacag
     6481 ctcttcgtaa agtttctcgc caggacgcag cccggtataa acgatttcga catcctcccc
     6541 cggggttttg ccggacaaac gtatgagctg ttcggcgagg taggaaatct taaccggttc
     6601 acccatgtcc agcacgtata tttcccctcc cttcccgata acgcctgcct gcaggatgag
     6661 ctggcttgcc tccgggatgg tcataaagta gcgttgaata tccggatggg tcactgtcac
     6721 cggaccgccc tgcgcaatct gctgctgaaa caggggaata acgcttcccg aggaacccag
     6781 tacgttgccg aagcggactg tgatgaaacg ggtctttgat ttggcattca tgccctggca
     6841 atacatctcc gccacgcgct tgctggcacc catgacattg gcggggttca ccgccttgtc
     6901 cgtcgagacc atgacaaacg actcgcaacc gaatttgtcc gccagattcg ccactacacg
     6961 tgtaccaaaa acgttattga ccacggcggc acgcgcctga tcctccagga tcggcacgtg
     7021 cttgtaggcg gcggcgtgat aaatgacgga aggatgataa gcacgcaata tcttttccat
     7081 ctgcaccacg tcattgatat ccaccaataa gctaaccaac gaaagctctg gcatgccctg
     7141 ccgaagctca atgtcaatac tgtataaatt gaactcgctg cgatcgagga taatcagttg
     7201 gctcggtttg agtcgtgcaa gctgacgaca aagctcggcg ccgatggatc cacccccacc
     7261 ggtcacgaga accgtctttc cgcgcgtcgc ctcggtgatg gcctgccaat caagctccac
     7321 cggctcgcgg cctagcagat cttcaatcct gacgtcacgc aaatccttca ggcttgcctt
     7381 tccgctgacc agatcctgca tttgcggcag gatacggaag ggcaatcccg tggtttcaca
     7441 gatttcaacg atacgccgaa tctggcgcga ggtggcggcc ggcaatgcaa taatgatcaa
     7501 atcaataccg tgttttgtaa cgatttgcgg tatctggtaa caggaaccca caacaggaat
     7561 tccatgaatt tcctttccgg ccttgccacg atcatcgtcg gcgaatgcca cgggaagata
     7621 cgcgccggag ggatctctca gcaggtcccg cgccagggtt tcaccggcct tgccggcgcc
     7681 gacgatcagc gcgtttttcc tttctgcatt cgcagtcagg ctgcgatcct gaatccattg
     7741 ataaagactg tgatccttga aaaggcgata gataaatcgt ggaccgccca gcagcaacac
     7801 caacaggaaa ccgtcaagca caaaaaccga gcgcggcacg ctttgtagcc gggtaagtat
     7861 gaaacttgct gccgcggcaa cgacaacgcc cgccgtaact gccttgagaa tgcgcacgag
     7921 atcgggaata gaagcaaacc gccagatacc gcgatacagg ccgaaatacc agaacattcc
     7981 gccctgcgcg acaacgatga ttggcagcaa cattagagcc tgattaaaaa aaccttccgg
     8041 gatcgaatcc agattgaatc gcagccagta cgccccgaac caggcgagcg gcaccatcag
     8101 aagatcgtgc gcaaaaaccg ctgtgcgtga tcgtaaatat aaaatgattg ccttcatctg
     8161 ctcatattcg tcgtatgttt gtatatttgt ttattacgct cgctcagagg ctaattgaat
     8221 ctaaattatt atagctaaat aaccccatga aatctcaggc tacccaataa atggggcatg
     8281 aagaccatat cttacggttc agacagaatc atgatgccgt ccggacgcat accacgaaac
     8341 ccaggtaaaa aaaataatat aaaaaagaac ccagcctgcg agcataaccg cctgtatcac
     8401 aactgttgcg cgcaggctta acaaggctgt tatcccgcag ccaatcatga tggcatattc
     8461 aattaacacc gttttgcggt gtcccaatcc tgcctgtacc agtttttgat agtaatgtgt
     8521 cttatgtgct tgccagattt tctcgccaca taacatgcgt ttaactaatg tcactgaagc
     8581 gtccgcgata aatggagaaa aaatcagtat tgcaacccaa aaaggaaaca cgccgtcacg
     8641 agcgccccat aacgaaaacg ccgccgccaa caaacccagc gatgatgaac caacatcccc
     8701 catgaaaatc cgcgcgggtg ggaaattaaa gaacaaaaac cccgccgctc cacccgtaac
     8761 cacaagacag gtttgtgcaa actgtaaatt ttcggtcaac agccccgtga ttgcgagaaa
     8821 tccgaaaccg ataacagcca tgcctgcggc aaacccatcc ataccatcca tgaaattgta
     8881 caggttgatc atccacacca cgtaagcaag gataaccagc aagccgaacc acggcagctc
     8941 cattcccggc aacaatccat tctcagagct aaaacttcca ctccatacca gcagcccggc
     9001 tccgacaacg tgtgccagca ggcgcaataa gaccgccaca ccggcacggt cgtccaggta
     9061 ggacacgatc gccaccggca caagtgctgc gagaaaccac atgtataaag cgaccccctg
     9121 gagaatccag attaacgcac cggcagccag cagaccggcc agaattgcta tccccccact
     9181 gcgcggcgtg ggattggagt gcagcgaccg ttcattcgga ttatcgagaa tatgaaacaa
     9241 cgaagatggg ttacagaaac gccacgacag ccaggcagac agaccgagcg ccgctatcgc
     9301 ggggacaaga aaaatcagca ttggcatgct agatcgtgag catggctaca aaagtgcaat
     9361 gtcttttgag atgttacggc gataccattc tccggtagcc tggagctcgc catccacaga
     9421 taacggcgga acccatccga gcaattgtcg caccttgccg gaatcaactt tcagtgatcc
     9481 gcatagccgt tcacagacac tgtcctttcc cagaacgcgc gccatcgtac gtaatagtga
     9541 aaccggaagc ggaaacaatc ttgatctttt ccccagcgca tgtgcgactc gttgaaataa
     9601 atccggtgta gacaggtctt cgccatccga taccaggaag acctctcctg cggcgtccgg
     9661 atgagaaagg catctgatga taaggcttac gaggttgctg aggccaacaa aactgcgctg
     9721 attgcgcaca ctcgccaacg gcagcggaat gcctcgatcg atccatttca ggatggtcag
     9781 aaaattacca ccgacacctg gcccgtacac gagtggtggc ctgataatga caatttccat
     9841 acccgtttcg gatgcgatac ggcgtaatcc ctgctctgcc tcccatttgg ataccgagta
     9901 tggatctgac gggcgcggtc gatcgtcttc gcggaatggc atgctggtcg ttgcttcacc
     9961 atttaccttg atcgtgctta cataaacaaa gcgccgcact ccggcctgcg ctgccatgga
    10021 tgcataccta agcgtgccta gagtgtttac acgacggaat tccatcaagg gatccaatgc
    10081 ttgctctttc atgacatgaa cgcgcgcggc gagatgcacc acggcttgaa tcccgtccag
    10141 gctggttccc cagtcagtct tcgggccgat atccccgaca atatgcacgg agagaggctc
    10201 actggaatta gatagaataa tgtcactttc gcggcgaaca gcggcgcgta cctcaacacc
    10261 acgatgtagc agttctgcaa tcagtgcctt accgacaaac ccgttcgcac cggtgacgaa
    10321 tgtcattcta gccggcatgc ctgtctctac gcggaccatc caacgccggt caggcgacgc
    10381 cacgcgatgt cgaaatacgc tttcgaggtg aaaaaccgga gagcgctctt cacatgccac
    10441 ctgaagtacc tccaatctcg gtggctctgc cgctggcttg catgcaccac acgagccgct
    10501 gggcacaatc gcacaccata acccgcgagc cgcaatcgcg cacagaaatc gacatcctca
    10561 taataaa
//
LOCUS       DBNGZE010000074         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_774539, whole genome shotgun sequence.
ACCESSION   DBNGZE010000074 DBNGZE010000000
VERSION     DBNGZE010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_774539"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="AC16FV_01850"
     CDS             complement(<1..1085)
                     /locus_tag="AC16FV_01850"
                     /inference="COORDINATES: protein motif:HMM:NF028500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2848837.1"
                     /translation="MDVSSLPVTALKGVGPKLADKLKRLGLQTVQDVLFHLPLRYQDR
                     TRLLRIGSLRPGMEAAVEGEIELADVVYRGRRSLICRVSDGSGHLHLRFFYFNAIQQQ
                     NLARGRRLRLFGEVRFGPVGLEMIHPEYEFIDGAQAPKAEENLTPVYPATTGVHQLSL
                     RKLARQALEKHLEKIHEWLPPAVLQELQLPTLTEALALVHQPPPHTPVELLAEGKHPA
                     VLRLSFEELLAHHLSLRRLRARVQMEQAPPITGAGELMRRLLAHLPFQLTRAQQRVLG
                     EILQDLQQRHPMQRLVQGDVGSGKTIVAACAGLGAIESGLQVAVMAPTELLADQHLRN
                     FSGWLEPLNLTVVGLSGKLNGRARQDAL"
     gene            complement(1173..1607)
                     /locus_tag="AC16FV_01855"
     CDS             complement(1173..1607)
                     /locus_tag="AC16FV_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR2848838.1"
                     /translation="MTRAVIKTDKAPQAIGTYSQAVKVGDTVYLAGQIPLVPETMALV
                     DGDMRMQIRRVFENLAAVAKASGGGLQDVVKLNVYLTDLGHFPLVNEVMAQYFREPYP
                     ARAAVSVAALPKGAAVEMDAVMVLGSARSPKKAKKKAKRNRR"
     gene            complement(1624..>3315)
                     /locus_tag="AC16FV_01860"
     CDS             complement(1624..>3315)
                     /locus_tag="AC16FV_01860"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR2848839.1"
                     /translation="KPERRRAIARETLDIYAPIANRLGVRQWAAELSDLGFSALYPLR
                     YRIIDNAVRKRHGNRKAIVEKIRIAIISQLEREGLSAEVTGREKSLYGVYRKMEQKHL
                     SFDQVYDIYGFRVIVDKADTCYRALGVIHNLYKPIPGRFKDYIAIPKANGYQSLHTVV
                     FGPFGVSLEVQIRTHEMNRVAEAGVASHWLYKNGDHGTDPTQQRALQWLKDLLDTQQK
                     AGNPSEFLEHLKVDLFPDEVYVFTPNGEIKKLPRDATVIDFAYDVHTDVGNRCVGAKV
                     NHQLVPLRTTLRNGDHVEIITASFGRPSPAWLNYVVTSKARAHIRNFLKNQKLDESAK
                     LGERLLVTALEDIGADLQKLSDETRQALLKNLKLKTWNDLLGEIGLGNRLASVVARQL
                     VPQTKEDESRFLRLFRRRGPKPKEPALAIHGTEGVVVNYARCCRPIPGDPILGFLTAG
                     RGIVVHTEDCPNVVRYRKHPEQWIDVQWEKDIKGVYPVYIRVEVKNQRGVLASVAAAI
                     SEEDANIDTVSFDDRDGQYTSMDFTVEVRDRVHLAHIMRRIRALESVVRINRKKG"
BASE COUNT          558 a         1027 c         1061 g          669 t
ORIGIN      
        1 agcgcgtcct ggcgcgcacg gccgttgagc ttgccggaca gcccgaccac ggtgaggttg
       61 agcggctcga gccagccgga aaaattgcgc aggtgctggt ccgcgagcaa ctccgtcggt
      121 gccatgacgg ccacctgcaa gccgctttcg atggcgccga gaccggcaca agccgcgacg
      181 atagtcttgc ccgagccgac gtcgccttgc accagtcgct gcatgggatg gcgctgttgc
      241 aggtcttgca gaatttctcc gagcacgcgc tgctgggcgc gcgtcagctg gaacggcagg
      301 tgcgcgagca agcgtcgcat cagttcgccc gcgcctgtga tagggggcgc ctgttccatc
      361 tgcacccgcg cgcgcaggcg tcgcaggctc aggtgatgcg ccagcagttc ctcgaaggac
      421 aggcgcagca cggcgggatg tttgccttcc gccagcagtt ccaccggcgt atgcggcggc
      481 ggttgatgca ccagcgccag cgcctccgtg agcgtcggca gttgcagttc ctgcaacacg
      541 gcgggcggca gccactcgtg gattttttcg aggtgttttt ccagcgcttg acgtgccagc
      601 ttgcgcaggc tcagctggtg cacacccgtg gtcgccggat acaccggcgt cagattttcc
      661 tcggccttcg gggcctgtgc gccgtcgatg aattcgtact cgggatggat catctcgagc
      721 ccgactggac cgaagcgcac ctcgccgaac agccgcaggc gtcggccgcg cgcgaggttc
      781 tgttgctgga tggcgttgaa atagaaaaac cgcagatgca ggtgaccgga gccgtcgctc
      841 acgcggcaaa tcaggctgcg tcggccacga tagactacgt ccgccagctc gatctcgccc
      901 tcgactgcgg cctccatgcc gggccgcaga cttccgatgc gcagcagacg tgtgcggtcc
      961 tggtagcgca gcggaagatg gaacaggaca tcctgcacgg tttgcaggcc gagtcgcttg
     1021 agcttatccg caagtttcgg accgacccct ttgagggccg ttaccggcag cgaggaaaca
     1081 tccatatgat ttttgaaacc gcggatgaac gcagattaac gcggataaca aatcttgcag
     1141 ctttttgcct ttcgatctgc gtccatctgc gtttatctgc ggttacgttt tgcttttttc
     1201 ttcgcctttt tcggtgagcg tgcggacccc agcaccatca ccgcgtccat ttcgaccgcc
     1261 gcgcccttgg gcaaggcggc gacgctcacg gccgcgcgcg ccgggtaggg ctcgcggaaa
     1321 tactgcgcca tcacttcgtt caccagcggg aagtggccga ggtcggtgag atacacgttg
     1381 agcttcacca cgtcttgcag gccgccaccg gatgccttgg ccaccgccgc cagattttcg
     1441 aagacgcgcc ggatctgcat gcgcatatcg ccgtccacca gcgccatggt ttccggcacc
     1501 agcggaatct ggccagcgag ataaaccgtg tcaccgactt tgaccgcctg ggagtaagtg
     1561 ccaatggcct gtggagcttt atctgtcttg attacggcgc gggtcatttc tgttcctcgt
     1621 tattcagcct ttcttgcgat taatcctgac gaccgattcc agcgcgcgga tgcgacgcat
     1681 gatatgcgcg aggtgcacgc ggtcgcgcac ctcgacggtg aaatccatgg aggtgtactg
     1741 gccgtcacga tcgtcgaagg acaccgtgtc gatgttggcg tcttcctcgg agatggcggc
     1801 ggccacggac gcgagcacac cgcgctggtt cttcacctcg acccggatat acaccgggta
     1861 cacgcccttg atgtccttct cccactgcac gtcgatccat tgctccggat gcttgcggta
     1921 acgcaccacg ttcggacaat cctcggtgtg caccacgatg ccgcgcccgg cggtgagaaa
     1981 gccgagaatg gggtcgcccg ggatcggccg gcagcagcgc gcatagttga ccaccacgcc
     2041 ctccgttccg tgaatcgcca gcgccggttc cttcggcttc ggaccgcggc ggcggaacag
     2101 ccgcaggaaa cggctttcgt cctccttggt ctgcggcacc agctggcgcg ccaccacgga
     2161 agccagacgg ttgccgaggc cgatctcgcc cagcagatcg ttccaggttt tgagcttgag
     2221 gtttttgagc agggcctgac gggtttcatc ggaaagtttc tgcaggtcgg cgccgatgtc
     2281 ttcgagcgcc gtcaccagca ggcgctcacc gagtttggcc gactcgtcga gcttctggtt
     2341 cttgaggaag ttgcggatat gcgcgcgcgc cttgctggtc accacgtaat tgagccaggc
     2401 cgggctgggg cggccgaagg acgcggtgat gatctccacg tgatcgccat tacgcagcgt
     2461 ggtgcgcaac ggcacgagct ggtgattgac cttggcgccg acgcagcggt tgccgacgtc
     2521 ggtgtggacg tcgtaagcga aatcgatgac ggtggcgtcg cgcggcagtt tcttgatctc
     2581 gccgttgggc gtgaacacgt agacctcgtc cgggaacagg tccaccttga gatgctccag
     2641 gaattcgctt gggttgcccg ccttctgctg ggtatccagc aggtccttga gccactgcag
     2701 cgcgcgctgc tgcgtcggat cagtcccgtg gtcgccgttc ttgtacagcc agtgcgaggc
     2761 gacgccggcc tcggccacgc ggttcatttc gtgcgtgcgg atctgcacct cgagcgaaac
     2821 gccgaacggg ccgaacacca cggtgtgcag cgactggtaa ccgttggcct tggggatggc
     2881 gatgtaatcc ttgaagcggc cggggatggg cttgtacaga ttgtggatca cgccgagcgc
     2941 gcgatagcag gtgtcggcct tgtccacgat cacgcggaaa ccgtagatgt cgtacacctg
     3001 atcgaacgac agatgttttt gttccatctt gcggtacacg ccgtacaggc ttttttcgcg
     3061 cccggtcacc tcggcggaca agccctcgcg ctccagctga ctgatgatgg caatgcggat
     3121 tttctcgacg atcgccttgc gattgccgtg gcgcttgcgc accgcattgt cgatgatgcg
     3181 atagcgcagc gggtaaagcg cggaaaaccc gaggtcgctc agttccgccg cccactggcg
     3241 cacgcccagg cggttagcga tcggcgcgta gatgtcgagc gtctcgcgcg cgatggcgcg
     3301 gcggcgctcg ggctt
//
LOCUS       DBNGZE010000075         3089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_913209, whole genome shotgun sequence.
ACCESSION   DBNGZE010000075 DBNGZE010000000
VERSION     DBNGZE010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_913209"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..307)
                     /locus_tag="AC16FV_01865"
     CDS             complement(17..307)
                     /locus_tag="AC16FV_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848840.1"
                     /translation="MTDTTAATAKANRPSMPRPFKVARVEKTDAPDGGQGDWYRYVLE
                     SGRSTITGQRCGSRKDVLAYATQCAEQLNTRGLTGQSIWSPRGRKPAASSST"
     gene            complement(726..1370)
                     /locus_tag="AC16FV_01870"
     CDS             complement(726..1370)
                     /locus_tag="AC16FV_01870"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR2848841.1"
                     /translation="MKLILLGAPGAGKGTVAKQLTQLDGSVQISTGDILRGAVQAGSD
                     LGKQAKAYMDRGDLVPDELIMGIMEKRLQEPDCKKGFLLDGFPRTIPQAEDLKKLLVK
                     LGIKLDMAVNIDVPRNVILDRLTTRRTCSNPKCQAIYNVKSMPPKKEGICDKCGSPVV
                     QREDETEAAISHRLDTYNKKTAPLIGFYDKEGMLLTVSATSSEAVIAAIKQKLG"
     gene            1743..2780
                     /locus_tag="AC16FV_01875"
     CDS             1743..2780
                     /locus_tag="AC16FV_01875"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR2848842.1"
                     /translation="MPGKVATTLISRILLFGVVPSMMALPVHAQWLTGEEAIMGTAIR
                     VELWADDKAAGEAAIAAVMAEMHRIDREMSPYKPESELSRINREAANHPVPISAEMFD
                     ILSRSIEFSKLSGGAFDITFSSVGYMYDYRRHIKPTDKEIEQALPGINYRHILLDAKR
                     RTVRFARPGVRIDLGGIGKGYAVDNSIALLKQRGITQAIVTAGGDSRVLGDRGGRPWM
                     VGIRDPRRKDNVVAVIPLVNSAISTSGDYERYFEADGVRYHHILNPKTGRSATGVHSV
                     SVIGPDATTTDGLTKPVFILGPEKGLRLIESIPGIDAVIIDADGKMFYSSGLQNRKRE
                     SSAPAKSTPLP"
     regulatory      2966..3059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          658 a          868 c          914 g          649 t
ORIGIN      
        1 cagaatttat tcttgattat gtggacgatg acgccgcggg cttgcggccg cgcggactcc
       61 agatggattg ccccgtcagt ccacgggtgt tcagctgctc ggcgcactgc gtggcatagg
      121 cgagcacgtc cttgcgcgat ccacagcgct gtccggtaat ggtggagcgg ccgctttcga
      181 gcacataacg gtaccaatct ccctgacctc cgtccggggc gtcggttttt tcgacgcgcg
      241 ccaccttgaa cgggcgcggc atggaagggc gattggcttt ggcagtagca gcagttgtgt
      301 cagtcattta gtagaaccaa gagcgtattc cgcagttata accgcggaag ggttagttaa
      361 agacgtttca aacagggaaa atgcagcaaa caaggcggct cgaacaaccg agacagcctc
      421 aaaaaaaagc attctaccac aatgagttcc ccgatgggac cccttcccaa gacatatttt
      481 gcctggctgg cggtcaaata ccggggtttc cgacggtttg cgccggcaat ggagcacgcg
      541 caggatagga agcacttggg catatcaccc ggcgcgcgcc gaaataacct gccagtctgg
      601 ccgacaatat tccgacgagt agcgcgggcg gcaagctgca acggccactt ggggcggcat
      661 tcaagcagtc aggcgggaaa atgaaaaggc cggtagcggc gggagccggc cttttcttcc
      721 gggttttatc cgagtttttg tttgatcgcc gcgatgactg cttcactcga tgtggcgctg
      781 accgtgagca gcatgccttc cttgtcatag aagccgatga gcggagcggt tttcttatta
      841 taagtgtcga gccggtggct gatggcggct tcggtctcgt cctcgcgctg taccacgggg
      901 ctgccgcatt tgtcgcagat gccttccttc ttcggcggca tgcttttgac gttgtaaatc
      961 gcctggcact tgggattgga gcaggtgcgg cgggtggtga gacggtcaag gatcacgttg
     1021 cgcggcacgt cgatgttcac cgccatgtcg agcttgatgc cgagttttac cagcagcttt
     1081 ttcagatcct cggcctgggg aatggtgcgc ggaaaaccgt cgagcaggaa gccctttttg
     1141 caatccggtt cctgcaggcg cttttccatg atgcccatga tcagctcgtc cggcaccagg
     1201 tcgccacggt ccatgtaggc cttggcctgt ttgccgagat cggagccggc ctgcaccgcg
     1261 ccgcgcagga tgtcgccggt ggaaatctgc accgaaccgt ccagctgtgt cagctgcttc
     1321 gcgaccgtgc ccttgcccgc gcccggggcg cccaacagaa tcaacttcat ggattttttc
     1381 ctctcttaga tttgaaccag tacgaaacct gaaatcaggc tgcggccccg cctgttggcc
     1441 gcgaaggggc gcattttgcc cttggtctgc cgccaagatc aacaggaaag tagggaaatc
     1501 tccgtgccct atgccggaat ccccgccgcc acgcccggat tacccatgcg agagctccca
     1561 taccgcaatg aaaatccgga tgtccggaat ccctgatatt caggctggat agtccgataa
     1621 tcgtctgagg tatgcagaga gatgccgggc tcaaatactc tacaatcggc aaatgccggg
     1681 ttgtccggca tcggattact cattcagacc ttggggaccg aaatggagat ggatggtcga
     1741 ccatgcctgg taaggtagct accactctca tatcaagaat cctgctattt ggcgttgttc
     1801 catccatgat ggcgctgccg gtgcatgcgc aatggctgac cggcgaggaa gcaatcatgg
     1861 ggaccgctat acgcgttgag ctatgggcgg atgacaaagc agcgggcgag gcggcgatag
     1921 cggcggtgat ggcggagatg caccgcatcg acagggaaat gagcccgtac aaaccggaga
     1981 gcgaactgtc gcgcatcaac cgcgaagccg ccaatcatcc tgtgccgatc agcgcggaga
     2041 tgttcgacat tctttcgcgc tcgatcgagt tctcgaaact cagcggtggt gcgtttgaca
     2101 tcactttttc cagcgtcgga tatatgtacg attatcgtcg tcacatcaag ccgacggaca
     2161 aggagatcga gcaggccctg cccggcatca actatcgtca tatcttgctt gacgcgaagc
     2221 gacggacagt cagattcgcg cgacccggcg tacgcatcga tctgggcggg atcggcaagg
     2281 gttacgccgt ggacaacagc atcgcgctgc tcaagcagcg cggcatcaca caggccatcg
     2341 tcaccgccgg cggcgacagc cgcgtactgg gagatcgggg agggcggccg tggatggtgg
     2401 gtattcgcga cccgcggcgg aaggataatg tggttgccgt gatacccttg gtcaattcgg
     2461 cgatctcaac ctcgggtgac tatgaacgtt acttcgaggc tgatggcgtt cgctatcacc
     2521 acattctcaa tcccaaaacc ggtcgctctg ctaccggcgt gcacagcgta tcggtcattg
     2581 gtcccgacgc aaccaccacg gatggtctca ccaagccggt gtttattctg ggaccggaaa
     2641 aaggattgcg cctgatcgaa tccatacccg ggatcgatgc ggtgatcatc gacgccgacg
     2701 gcaagatgtt ctactcctcg ggtttgcaga acagaaaacg ggaatcttcc gcacccgcca
     2761 aatccacccc gctgccatag tgtgtcacac cgtccatcgg gcgctttctt ccgcacggaa
     2821 gcattgctgc cgataacgct tttattctga atacggctct ggaaacatcc gtctgagatt
     2881 accgatacgc gcgaggatag ccgcgacgaa gggcggcatg cgcctaaacc tgagtacata
     2941 gaataagtat cgttgttgat aacagctttg ttcagggcat agccaccgaa aggtggtgac
     3001 gcaaagcccc gaggctaacg gtgagcagca tcgtcaccca tgccggtcgg gccgccgaag
     3061 cgacattaga cgcaaataga ttcccggta
//
LOCUS       DBNGZE010000076         3666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_566211, whole genome shotgun sequence.
ACCESSION   DBNGZE010000076 DBNGZE010000000
VERSION     DBNGZE010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_566211"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16FV_01880"
     CDS             complement(<1..317)
                     /locus_tag="AC16FV_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR2848843.1"
                     /translation="MAIIATKGTLDWAYPPFILASTGSALGWDVSIFFTFYGLLLLKK
                     DLGAEVSPLGNPAMPMKMPFGPKWFQNFVWPMPNLIMAGIPGFEKVATVLMKKTFKNK
                     GVA"
     gene            1014..2096
                     /gene="rfbB"
                     /locus_tag="AC16FV_01885"
     CDS             1014..2096
                     /gene="rfbB"
                     /locus_tag="AC16FV_01885"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR2848844.1"
                     /translation="MTDKPILVTGGAGFIGSNFVLKWIGQEGSPVINLDKLTYAGNIG
                     NLAKLKNDPRHIFVKGDITDQALVRELLNRHRPCAVVNFAAESHVDRSIHGPEDFIQT
                     NVNGTFHLLQESRAYWEKLAGNEKTVFRFLHISTDEVYGSLGPGDAPFTESTPYAPNS
                     PYSASKAASDHLVRAFHHTYGFPTLTSNCSNNYGPYQFPEKLIPLVILNALNRKPLPV
                     YGDGLNVRDWLYVEDHCQALRNILTKGRPGEVYNIGGCNEKTNIDVVRNICALLDELN
                     PASDRKPYASLITFIKDRPGHDRRYAIDARKIERELGWKPAESFETGIRKTVKWYLAN
                     MAWVKSITSGEYQKWLDTNYSNRGMA"
     gene            2093..2968
                     /gene="rfbA"
                     /locus_tag="AC16FV_01890"
     CDS             2093..2968
                     /gene="rfbA"
                     /locus_tag="AC16FV_01890"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR2848845.1"
                     /translation="MKGIILAGGSGTRLHPVTQAVSKQLLPVYDKPMIYYPLSTLMLT
                     GIRDFLVISTPQDVPRFEQLLKDGAEWGLNISYAVQPKPEGIAQAFIIGEQFIGNDSC
                     ALVLGDNIFYGHELTTDLQNAARKQTGATVFAYPVQDPERYGVVEFDSKKRALSIEEK
                     PKTPKSRYAVTGLYFYDNQVVRIAKSLKPSGRGELEITDVNIRYLERGQLDVMVMGRG
                     IAWLDTGTHESLLEASLFIETIEKRQGLKIACPEEIAYRMGYIAAGQLEKLAGRMSKN
                     GYGRYLLGVLQEQVF"
     gene            2968..3510
                     /gene="rfbC"
                     /locus_tag="AC16FV_01895"
     CDS             2968..3510
                     /gene="rfbC"
                     /locus_tag="AC16FV_01895"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR2848846.1"
                     /translation="MNVIQTAILDVLIVEPKVFMDTRGFFYESFNKKAMQTAGIAGDF
                     VQDNHSRSARNVLRGLHYQITQPQGKLVRAVAGEIFDVAVDIRRRSPTFGQWVSMNLS
                     AQNRRMAWVPPGFAHGFLVLSEFAEVLYKTTDYWAPQSERTIVWNDHDLAIAWPIGEE
                     PQLSAKDKAGTAFSEAVVFE"
     gene            3507..>3666
                     /locus_tag="AC16FV_01900"
     CDS             3507..>3666
                     /locus_tag="AC16FV_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HIR2848847.1"
                     /translation="MRKILLTGENGQVGWELQRTLASLGDIVTVGHQEMDLADPDSIR
                     TVIRHVRPD"
BASE COUNT          863 a          928 c         1018 g          857 t
ORIGIN      
        1 ctggcgacgc ccttgttctt gaaggtcttc ttcatcaaca cggtggcgac tttttcgaat
       61 cccggaatgc cggccataat gagattcggc atgggccaga cgaaattctg gaaccacttc
      121 ggaccgaacg gcattttcat cggcatggcc gggttgccga gcgggctgac ttccgcgccc
      181 aaatcctttt tcaggagcaa caggccgtag aaggtgaaga aaatcgatac atcccatccc
      241 agggcggagc cggtggaggc gaggatgaac gggggataag cccaatccag ggtgcccttg
      301 gtagcgatga tcgccatgct cggcgtcttg ttggcctcga tttcttccag tttcttggga
      361 agaaccttat cgaggtgctt gctgatcatc tgttcgagat ttgcttcttg catcgtggtt
      421 gacatttacc ttcctccaat acgtttaagt atgcgcggcg ttgtgtggtt gatatgtggt
      481 tacgcccgct ctgggaacga gtgcgtcacc cctcgtagcc ttaactgctt gatggctatc
      541 tgatttttca cagttcagac aaacagagag cgctaatatg ctgaggccga tgctattagt
      601 caagcttgga atatggccca agctccctgg ttggacagat aggcacagat tgatccctgt
      661 tctcacgcaa ctcaggttat ccggctatcc tattgattga tatatggatt tatattttct
      721 aactgttgga gtggccgttt tctgggcgac gtatcaggca tgcatcggct ggtccgattg
      781 cgaacatata ctgatattat tcgtcacttc ggcggcgctg atagagtccg ctaagcatgg
      841 atcatcgaat cggattttag cagcacaaaa attaaagatc tttttcgccc cctgccatca
      901 gacatgtaaa tacaattctg tagaaattcc ctccgatacc ggcttttacc gattgccgaa
      961 ggtcgagagt ttctaatcaa tcgagtccgg caagctattg caagtggtta agaatgactg
     1021 acaaaccgat actggtcacc ggcggtgccg gattcattgg ttctaatttc gtcttgaagt
     1081 ggatcgggca ggaaggctcg ccggtgatca atctggacaa gctgacttac gccggaaata
     1141 ttgggaacct ggctaaactg aaaaacgatc cgagacacat attcgtaaag ggggatatca
     1201 ccgatcaggc attggtccgg gaacttttaa accgtcatcg accgtgcgcc gttgtaaact
     1261 ttgcggcgga aagccatgtc gaccgttcca tccacgggcc ggaggatttc attcagacca
     1321 atgtgaacgg cacttttcat ttgctgcagg aatcccgggc ttactgggag aagctcgcag
     1381 gaaatgaaaa aaccgtcttc cggtttctgc acatatcaac agatgaggtg tacggttcac
     1441 tgggtcctgg ggatgccccc tttacggaat ccactcctta cgcgccgaac agcccgtatt
     1501 ccgcctcgaa ggcggcttcg gatcatctgg ttcgcgcctt tcaccacact tacggttttc
     1561 ccacgctgac gtccaattgc tcgaacaatt acggccctta tcagtttccg gaaaaattga
     1621 ttcccctggt aattctgaac gcgctgaacc gtaaaccctt gcctgtctac ggagacggtc
     1681 tcaacgtgcg tgactggctg tatgtggagg accattgcca ggcgctgcgg aacattctga
     1741 caaaaggaag gcccggagag gtttataata ttggtggctg taatgaaaaa acaaacattg
     1801 atgtcgtgcg caacatttgc gcattactgg atgaactgaa tccggcttct gacagaaagc
     1861 catacgcctc gctcatcaca ttcataaaag accggccggg acacgatcgg cgttatgcca
     1921 tcgatgcgcg caagatcgaa cgagagctcg ggtggaaacc agcggaaagc ttcgaaaccg
     1981 gaatacgaaa aaccgtgaaa tggtatctcg ccaacatggc ctgggtaaaa agtataacca
     2041 gcggtgaata ccagaaatgg ctggacacga attacagtaa ccgggggatg gcatgaaggg
     2101 cattattctg gcgggagggt cggggacccg gctgcatccg gtaacacagg ccgtttccaa
     2161 gcaattgctg ccggtttacg acaaacccat gatttattat cccttgtcca cgctgatgtt
     2221 gacgggaatc agggattttc tggttatctc taccccccaa gacgttcccc gatttgagca
     2281 attgctcaag gatggcgcgg aatggggact gaatatttcc tacgcggtgc agcccaagcc
     2341 cgagggcatc gcgcaggcgt tcattatcgg cgagcaattc atcggcaacg attcgtgcgc
     2401 attggtgttg ggtgacaaca tcttttatgg gcacgaattg accaccgacc tgcaaaacgc
     2461 cgcgcggaaa cagacgggcg caaccgtctt tgcctatccg gtgcaggacc cggagcgcta
     2521 tggcgtggtg gagtttgact ccaaaaaaag ggcattgtcg attgaagaaa agcccaagac
     2581 gcccaaatcc cgttatgccg ttaccggcct gtatttttat gacaatcagg tcgtgcggat
     2641 tgcaaaatct ctcaaaccat cgggccgtgg cgagctggaa atcaccgacg tcaacatacg
     2701 ctatcttgag cgcgggcaac tcgatgtcat ggtcatgggg cgcggcatcg cctggctcga
     2761 tacgggtact catgaatccc tgctggaggc gtcgctcttc atcgaaacca ttgagaagcg
     2821 gcaggggctc aagatcgcct gtccggaaga aatcgcctat cgcatgggtt atatcgctgc
     2881 cgggcagctt gaaaaactgg ccggccgcat gagcaagaac ggttatggcc gatatctgct
     2941 cggggtgctg caagaacagg tgttctgatg aatgtcatcc agaccgccat cctcgacgtg
     3001 ctgatcgtgg agccgaaagt cttcatggac acgcgcgggt ttttctatga gagcttcaac
     3061 aaaaaggcca tgcagacagc gggtatcgcg ggagatttcg tgcaagacaa tcattcgcgc
     3121 tcggcgcgga atgtgctgcg cggcctgcat tatcagatca cgcaaccgca gggcaagctg
     3181 gtgcgggcgg tcgccggcga gatattcgac gtcgccgtgg atatccgccg acgctcgccg
     3241 acgttcggcc agtgggtgag catgaatctg tcggcacaaa acaggcgcat ggcgtgggtc
     3301 ccgccaggtt ttgcgcacgg ttttctggtt ctgtccgaat tcgccgaggt tctgtacaag
     3361 actaccgatt actgggcgcc gcaatccgag cgtaccattg tgtggaacga ccacgatttg
     3421 gcgattgcct ggccgattgg agaggagccg caattatctg ccaaggacaa ggccggcacg
     3481 gcattcagcg aggcggtggt gttcgagtga gaaaaattct tctcaccggg gaaaacggcc
     3541 aggttggatg ggaattgcaa cgtacgttgg cctcgttggg tgatattgtc accgtcggcc
     3601 atcaggaaat ggatctggcc gacccggatt ccatcagaac ggtaatcagg catgtccggc
     3661 cagatc
//
LOCUS       DBNGZE010000077         3287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1469266, whole genome shotgun sequence.
ACCESSION   DBNGZE010000077 DBNGZE010000000
VERSION     DBNGZE010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1469266"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..787
                     /gene="thiE"
                     /locus_tag="AC16FV_01905"
     CDS             146..787
                     /gene="thiE"
                     /locus_tag="AC16FV_01905"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR2848848.1"
                     /translation="MVNKSRIAGLYAIADTRYLDDARLLPAVSEAITGGARIIQYRDK
                     SHDAQARLTQAKKITALCRQHDALFIVNDDVALAKETQANGVHLGREDTSLTQAREQL
                     GPLAIIGVSCYNELARAETAQAQGADYIAFGRFFPSRTKPRAVPANLDLLREAKKKLR
                     IPIVAIGGITPENGASLIAAGADALAVIEGVFGQVDVRAAAERYTSLFKNKQP"
     gene            784..2064
                     /gene="hemL"
                     /locus_tag="AC16FV_01910"
     CDS             784..2064
                     /gene="hemL"
                     /locus_tag="AC16FV_01910"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR2848849.1"
                     /translation="MTTSSDLFKRAQKCIPGGVNSPVRAFQGVGGDPIFFARGSGAYV
                     FDEDGKRYIDYVGSWGPMILGHAHPGVMAAVKETMEYGLGFGAPTRIEVEMAELICRL
                     VPSIKKVRMVSSGTEATMSAIRLARGFTGRDKIVKFEGCYHGHADSLLVKAGSGALTL
                     GVPTSPGVPADLASHTLTLEYNNSQQVRELFGKLGKEIACVIVEPVAGNMNCVPPVKG
                     FLETLRELCTQHEAVLIFDEVMTGFRVALGGAQAHYGIPPDLTTLGKVIGGGLPVGAF
                     GGRADIMGRLAPEGPVYQAGTLSGNPVSLAAGMATLKEISQPGFFEALTAKTTMLARG
                     LAERAKRAGVPLTVQHVGGMFGIFFTQEKSVTSFAQVMACDVERFRKFFHGMLREGVY
                     LAPSAFEAGFVSAAHTESDIDETLDKAEKVFKTL"
     gene            complement(2239..2475)
                     /locus_tag="AC16FV_01915"
     CDS             complement(2239..2475)
                     /locus_tag="AC16FV_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848850.1"
                     /translation="MLSHIVNLVNDYEVVHGARPNVVYMNETHYGYLREELPGVRDHD
                     NLVAILGVDIALSDEAIRPQVATVRFASENILVS"
     gene            complement(2752..>3287)
                     /locus_tag="AC16FV_01920"
     CDS             complement(2752..>3287)
                     /locus_tag="AC16FV_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2848851.1"
                     /translation="VAAGQAKAAVCAGCHGADGNGGADPLWPKLAGQDPDYIAKQLAD
                     FKSGARKDPIMAGMAAPLSSADMRNIGAYFASLKPKPGAARDAQMAKLGERIYRGGNA
                     KMAVSACMSCHGPSGHGIPPRFPKVSGQSTPYTNKQLLAFKAGSRTNDGDTMTRIAFR
                     MSEQEIKAVSEYMAGLH"
BASE COUNT          619 a          996 c         1036 g          636 t
ORIGIN      
        1 cgccgtgggc gaggccctgc gctacacctg ggagaccttg aagcacggct tccgccccgg
       61 catgggccaa ccgctgccga accggctgta ttgggcctgg gagatggatg aaccggagag
      121 cggcagcgac gattaactcc acataatggt taataaatct cgcattgccg gactctacgc
      181 catcgccgat acccggtatc tcgacgatgc gcggttgctg ccggcggtga gcgaggccat
      241 tacgggtggc gcgcgcatca tccagtatcg agataaatct catgacgcgc aggctcgttt
      301 aacgcaggca aaaaaaatca ctgcgttatg ccggcaacat gatgcgctgt tcatcgtcaa
      361 cgacgacgtg gcgctggcga aagaaacaca ggccaacggc gtgcatctgg ggcgggaaga
      421 cacctcgctg acacaggcgc gcgaacagct cggcccgctg gccatcatcg gcgtgtcctg
      481 ctacaacgag ctggcgcgcg ccgagacagc tcaggcgcag ggcgcggatt acatcgcctt
      541 cggtcgtttt ttcccttcgc gcaccaaacc gcgggcggtg ccggcaaacc tcgatctgct
      601 gcgcgaggcc aagaagaaac tgcgcattcc catcgttgcc atcggcggca ttaccccgga
      661 aaatggcgcc tcgctgatcg cggccggggc cgatgcgctg gcggtgatcg agggcgtgtt
      721 cggccaagtc gatgtccgcg ccgcggcaga acgatacaca agcctgttca agaacaaaca
      781 gccatgacta cttcctccga tctcttcaaa cgtgcgcaga aatgcatccc cggcggggtc
      841 aactcgccgg tgcgcgcgtt ccagggcgtg ggcggcgatc cgattttctt cgcgcgcggg
      901 agcggcgctt acgtcttcga cgaggatgga aagcgctaca tcgactacgt cggttcctgg
      961 ggaccgatga tcctcggcca tgcgcatccc ggggtgatgg cggcggtgaa ggaaaccatg
     1021 gagtacgggc tgggcttcgg cgcgcccacg cgcatcgagg ttgagatggc ggaactgatc
     1081 tgccggctcg taccttctat caaaaaggtg cgcatggtca gctccggcac cgaggccacc
     1141 atgagcgcga tccgcctcgc gcgcggcttc accgggcgcg acaagatcgt caaattcgag
     1201 ggctgctatc acggccatgc cgactcgctg ctagtgaagg ccggttccgg cgcgttgacg
     1261 ctcggcgtgc ccacttcgcc cggcgtgcca gcggatctcg ccagccacac actcacgctt
     1321 gaatacaaca acagccagca agtgcgcgaa ctgttcggca agctgggcaa ggagatcgcc
     1381 tgcgtcatcg tcgagccggt ggccggcaac atgaactgcg tgccgccggt gaaaggcttt
     1441 ctggaaacgc tgcgcgagct gtgcacgcag catgaggcgg tgctgatttt cgacgaggtc
     1501 atgaccggct ttcgcgtggc cctgggagga gcacaggcac attacggcat cccgcccgat
     1561 ctcaccacgc tcggtaaggt aatcggcggc ggtctaccgg tcggggcctt cggcgggcgc
     1621 gccgacatca tgggccgcct tgccccggaa ggaccggtgt atcaggccgg aacgctgtcg
     1681 ggcaacccag tgtcgctcgc ggccgggatg gccacgctca aggaaatttc acagccgggg
     1741 tttttcgagg cgctcacggc caagaccacc atgctcgcgc ggggcctcgc cgagcgcgcc
     1801 aaacgcgcgg gtgtgccgtt gaccgtgcag catgtcggcg ggatgttcgg aattttcttc
     1861 actcaggaaa aatcggtgac aagcttcgcg caggtcatgg cttgcgacgt cgagcgcttc
     1921 cggaaatttt tccacgggat gttgcgtgaa ggcgtgtatc tggcgccctc ggccttcgag
     1981 gccggcttcg tctcggcggc gcacaccgag tccgatatcg acgaaaccct ggacaaggcc
     2041 gagaaggtat ttaaaacact ttaagaattt ttgccgcaaa ggcgccaaga cgcaaagaaa
     2101 agactttttc gattcaaaaa acctttgcgt cctttgcgct cttcgcgtct ttgcgttgaa
     2161 aattattatc agccgttttc taaaataaaa cgggccggtg gtccggcccg tgtgtgcacc
     2221 ccgcacaaca gttcttgtct agctgaccag tatattctcg gaagcaaacc ggacggtggc
     2281 gacctgcggg cggatggctt cgtcgctcag ggcgatgtcc acaccgagga tggcgaccag
     2341 gttgtcgtgg tcgcgcacgc ccgggagttc ctcgcgcagg tacccgtaat gggtttcatt
     2401 catgtacacg acgttcggcc gcgcgccgtg cactacttcg taatcattca ccaggttcac
     2461 aatgtggctt aacatggttc gctccttttt gccccgtttg ggccgtcccg gaatcccgct
     2521 ttacccctgc cgtgattccg cgcttctcac tacttgaaag agcaatactc atgccatgcc
     2581 ggtcaatgac ctaacctatt gaaacagagt ggtatttatc agcagcgtcg ttgacgcgtt
     2641 gcggcagggg gcttggcagc ggccggacaa aggccggcaa ctgccgtcgc acggcagaaa
     2701 cattctgccg tgccagtatt gcagcgggat gaaaaggaga gctttagcgg gtcagtgcaa
     2761 accggccatg tattccgaca ccgccttgat ttcctgctcc gacatgcgga aggcgatacg
     2821 ggtcatggtg tccccgtcgt tggtccggct gccggccttg aaggccagca gttgtttgtt
     2881 ggtatacggg gtgctctggc cggatacctt ggggaagcgc ggcggaatgc cgtggccgga
     2941 ggggccgtga cagctcatgc aggcggatac ggccatcttg gcgttgccgc cgcggtaaat
     3001 cctctcaccc agcttggcca tttgcgcgtc ccgagccgcg cccggcttgg gcttgaggct
     3061 ggcgaagtag gcgccgatgt tcctcatgtc agcggagctg aggggggcgg ccatgccggc
     3121 catgataggg tccttgcgcg cgccggattt gaaatcagcc agttgcttgg cgatgtaatc
     3181 cggatcctgg cctgccagct tgggccacag cgggtcggcc ccgccgttgc catcggcccc
     3241 gtgacagccc gcgcataccg ccgccttggc ctggccggcc gcgacat
//
LOCUS       DBNGZE010000078        16210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_80060, whole genome shotgun sequence.
ACCESSION   DBNGZE010000078 DBNGZE010000000
VERSION     DBNGZE010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 16210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16210
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_80060"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="AC16FV_01925"
     CDS             complement(<1..1741)
                     /locus_tag="AC16FV_01925"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR2848852.1"
                     /translation="MAVRNSLQRVARFFLLPLSSLSDRLGNWFYVGLAVVVAAAATFA
                     IATGSTAGMKNKAYDLIMKTRFQHPAADADIVIVDIDESTLAAMAPEYGRWPWPRNIM
                     GELVEGIAAQQPKAIVFDIAFSDPDVFNVEGDKYFRSAIAATPNTFFPMIRLNEENDS
                     RSELRVMQLPGVSKLDLAASETATIAAILPFFFDALTDHRLGTNNIYTDEDGMVRNYE
                     VYANEYGWRVGSLPANVVSALGVSLPDQTDVLLNWRGKPPSYRRVSFHEIYFDLLKSK
                     KTRPADEFAGKIVIIGSTAPSLFDLKTTPVAKAHPGVEILASAIDNLKKGDFLTELPP
                     WIYILVTLVSVVLLTIAFVYNIDQRVVNLTFTVLQSGFLAVSYLTLNFSTIFVDLTAP
                     FTFSLAYFAVARFNSMFSGFRRSGQPFFSTALDEGNACRVVLVQCHVHLKNHRARLAL
                     GASLKKQVGLSRYGLVTSPFFKGMPLLHAFFRDATVFYWLVPEEKTSEALQDVVSVME
                     RSLPAIRKSARRASKDGPVVTWLVHGFSFAVDAEETWRLKGEEGMARLFSLADKVESA
                     RGGGAVRVRATKEF"
     gene            2017..2511
                     /locus_tag="AC16FV_01930"
     CDS             2017..2511
                     /locus_tag="AC16FV_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR2848853.1"
                     /translation="MELTIVRWLHIFAGITWIGLLYYFNFVQAVALPRAKADNTAAGI
                     TKHVAPQALLWFRWAAVATWILGAYYLERSGVGLGNAFLLKGSAAAIGVGAWLGTIML
                     FNVWVLIWPNQKKVLGLVQADDAAKAKAARVAFLTSRTNTMLSLPMLFFMVAGSVGHG
                     LPHM"
     gene            2655..3836
                     /locus_tag="AC16FV_01935"
     CDS             2655..3836
                     /locus_tag="AC16FV_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR2848854.1"
                     /translation="MSSYQMPTFAPLPVAFTHGEGAWLWDTKGKKYLDALAGIAVCGL
                     GHAHPAVKAALCDQAGKLLHTSNWYEIPLQEQLAERLCKLAGMDNAFFCNSGAEANET
                     AIKLARLYGHQRDILTPGIIVTEGSFHGRTLATLSATGNRKIQAGFEPLVQGFYRVPY
                     NDLAAVQQVGERNKDVVAVLVEPITGEGGVSIPDTGYLKGLRKICDERGWLLMLDEIQ
                     TGMCRTGKWFACQHEGVVPDVMTLAKGLGNGVPIGACLARGKAAQVFKPGSHGSTFSG
                     NPLVCRVALAVIDELEKGKFAERAAKLGERLLARLKEILGKVAGVKEIRGRGLMLAIE
                     LDRPCKELLQRALESGILINVTAENVIRLLPPLIFTDNETDMLVDKLDEVVRAFLRGP
                     T"
     gene            3946..4860
                     /gene="argF"
                     /locus_tag="AC16FV_01940"
     CDS             3946..4860
                     /gene="argF"
                     /locus_tag="AC16FV_01940"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR2848855.1"
                     /translation="MKPRHFLTLPDLTPAELKRVLARAVEMKAMLRRGERHMPLAGKT
                     MAMIFEKSSTRTRVSFQVGMAQLGGTSLFLSPRDTHLDRGEPVEDTARVLSRMVDIIV
                     IRANSHTMVERFAAHSQVPVINALTDLDHPCQLLADVQTWIEKRGDIAGRKVAWIGDG
                     NNVCHSWISAARLLGFHLDVATPKGYEPKSAIVVASKNHVTLHHDPKQAARGADIVVT
                     DTWASMGQEHEKAERIKAFAGFMVDGALMKLANKDAVFMHCLPAYRGMEVAAEVIDGP
                     QSVVWDEAENRLHAQKALLEFLLAGKQE"
     gene            4962..6044
                     /locus_tag="AC16FV_01945"
     CDS             4962..6044
                     /locus_tag="AC16FV_01945"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848856.1"
                     /translation="MNEILALNDIECRYDGRLAVCGLSMRVSAGSIVCLLGPSGCGKT
                     TVLRAIAGFHSLSAGDIWINGSAVSQPGFTLPPEKRHLGMVFQDHALFPHLDVAGNIG
                     FGLRGISDNQKLAIINEMLEVTGLNGMGERYPHELSGGQQQRVALARALAPKPDLILL
                     DEPFSNLDIELRERLSGEVREILKQRGTTGVLVTHDQHEAFALGDAVGVMHEGKLLQW
                     DTPYNLYHEPSNRFVADFIGQGVFLRGTLLTPDTMETEIGVHKGNRAYDWGRGAQIEL
                     LLRPDDIVPDPEGPLKAKVVQKAFRGADILYTLRLPSGGRLLALFPSHADHAIGELVG
                     IRVQADHLVAFPLAAGHSAGTTHATN"
     gene            complement(6086..7948)
                     /locus_tag="AC16FV_01950"
     CDS             complement(6086..7948)
                     /locus_tag="AC16FV_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HIR2848857.1"
                     /translation="MSGAHRLGTSRELNQPYGGTLINLLVSPERAAELKAVSRDLVSL
                     DLSPRHLADLEMLATGVFSPLTSFMGQADYEAVCAGMRLADGRLWPVPVTLDASSKFA
                     EQISPGLAIALRDGEGTLMAVITVSEIWQPDRGAESQCLYGTRSFMHAAANQLLNANG
                     DVCITGKLEVVELPVHHDFSPLRQTPAQVRAQLARRNVTRVVGYHPHHLMHRAHVEFT
                     KRTAYLKDAVLLIQAVVGRKGLNDADHFSRARALCTVLDHYSSQVAQLNLLELSPRHS
                     GVRSAMWHAILHKNFGCSHFIVEHDYDDQGEGATGEPLYGQYHGLESVAWHAQEIGIE
                     VVPFRNLVLEEDQPEKLLSPRGASRRMKGQIRETSHERNAEIAHWFSYPAVLEEWRKT
                     LRHGFTLFFTGLSGAGKSTLAQVLYARLKEHEARPVTLLDGDIVRKNISSELGFSREH
                     RDINVLRQGYVASLITRHGGIAICAPIAPYAKTRAQVRAMVEEDGDFIEIYVATPLAT
                     CETRDRKGLYARARAGLIKQFTGVSDPYETPANPEIVIDTSLQSANEAADKILAYLAA
                     HGYLAREAKAETGQNQMRHDPITLWTSTSGSVLPLTQSPDAAAPTLGSGKSFHK"
     gene            8351..10585
                     /locus_tag="AC16FV_01955"
     CDS             8351..10585
                     /locus_tag="AC16FV_01955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2848858.1"
                     /translation="MEKKILRLLIVDDSPDDAELAVASLRKAGFMLKNQRVQDLASMQ
                     VALDKGTWDVVLSEYTLPHFGAQLALDLVKRAHLDIPFLVVTRNIRDPDLVKIMHNGA
                     HDVVLKSQSARLAPAVERELHVAEERRKYQKMSLALNEVENKNRAIIEGSREAICYCQ
                     DGMHIDANKAYLEMFGYQDLSELEGVPVLNLVVKSDHVRFKDYLRKINSPSPPEPQEF
                     LAAKKDGQHFHAELAISPIALNGESCTQFVVIDVSKRKAVENRLQYLNQHDPLTGLYN
                     RHHFTQELTKALEKVKQDGGQSALLYLDLHELKEINDTLGHAAGDRLLLKIARLFREK
                     LGESTILARFSGDEFTALLHGMSEAQIKEAVAALLSALKETTFSEGGKTFRCDGSIGV
                     VVIDKNSENAQALLNKAYQTCLGNRPSKPAPVAAPAPAPETAPVSRSAPAAEAPAVAE
                     VPALAPIPIPTVTEIRRPAPAVSTVGAHDWEARLKTALEKDGFQLAYQPTVNLHGDPE
                     EYFEVLLRLAGRNGELIHAGEFMSAAEQSGLMGAIDHWVIRQSVESLAALHREGRKAS
                     FFVNLCPASLKDPELLSMLRKQVVAARIKPQYLVLEADEPAVMAHPSDAAMFMQAVKK
                     IGCRFAIDNFGNNLSTLNRLRDMPVDFLKISGSLIRNLSTDVLTYTSLKAIIDLAKSM
                     NKQTIAKFVEKADDLGVLWNLGFDYVQGNYFQQADAHTDYTFADETTLATDTSTPTWA
                     NSRR"
     gene            10694..12370
                     /gene="ettA"
                     /locus_tag="AC16FV_01960"
                     /pseudo
     CDS             10694..12370
                     /gene="ettA"
                     /locus_tag="AC16FV_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
     gene            12660..12938
                     /gene="bufA2"
                     /locus_tag="AC16FV_01965"
     CDS             12660..12938
                     /gene="bufA2"
                     /locus_tag="AC16FV_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BufA2 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR2848859.1"
                     /translation="MNAKKLTGLALATAAAGLFATAGIPAANAAKHEGEAKVHCDGVN
                     SCKGTSACQTASNACKGQNSCKGKGWLYMTKAECDAAQAKLKSEMHKK"
     gene            13001..13870
                     /gene="bufB"
                     /locus_tag="AC16FV_01970"
     CDS             13001..13870
                     /gene="bufB"
                     /locus_tag="AC16FV_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MNIO family bufferin maturase"
                     /protein_id="HIR2848860.1"
                     /translation="MTSAPALPERPWLGFGLGLRPAHYETVLNESLAVDWFEIISENY
                     IVPGGKPLHYLDRIRERYPMVMHGVSLSIGSQDPLNREYLKQLRALAKRIEPQWISDH
                     LCWTGAHGLNAHDLLPLPYTEEALKHVVRRVSEVQDFLGRRILLETVSSYVSYPESEM
                     SEWEFLREIATRADCLILLDINNIYVSSINHEFNPHHYLDAIPVERVWQFHLAGHRNL
                     GDYIIDTHDEPVIDPVWELYARAVRRFGRVSTMIERDDNIPPLPELLAELDHARHIAA
                     SVRQATAATAASA"
     gene            13867..14742
                     /locus_tag="AC16FV_01975"
     CDS             13867..14742
                     /locus_tag="AC16FV_01975"
                     /inference="COORDINATES: protein motif:HMM:NF021364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC/BufC N-terminal domain-containing protein"
                     /protein_id="HIR2848861.1"
                     /translation="MSTLAHLQSEFQSFLLDGNERMLNRVAGTAKVSAEQRLAIYYDA
                     YRLRLLEALTSNYPVLRAWIGDDEFEKAGIAYLAAHPSRHFSIRWFGHHLPEFLGATE
                     TWRDSPFLAEMASLEWTLSEIFDAEDSAVMSVEDMAHIPVKAWPDMRLHFHPAVRRLD
                     LRWNVPLIWKAINQNLDVERNTVSCRGETSATPSAGNARHAEAQHDIPAPEAGEHLQA
                     WLVWRQDLKTWFRSLSVDEAWALDAARGGESFAAICEGLCEWIDARNVALHAAGLMKQ
                     WITDGLVSEIDSSKQ"
     gene            14782..15273
                     /locus_tag="AC16FV_01980"
     CDS             14782..15273
                     /locus_tag="AC16FV_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR2848862.1"
                     /translation="MSQRMIFFGGFLICAGLMAAALYFQYGMKLEPCPLCIFQRLFVI
                     GVGLILLAGALHNPDKLGRRIYGGLTALMAALGAGVAGRHVWIENLPFDQQPGCGYSL
                     NDMLETFPLLKTLKLVLAGSGGCGQITWSFLGLSMAGWTLIFFIGFTVLGLMLVFTSL
                     GKR"
     gene            complement(15286..>16210)
                     /locus_tag="AC16FV_01985"
     CDS             complement(15286..>16210)
                     /locus_tag="AC16FV_01985"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2848863.1"
                     /translation="FEIPAWFKSSFLDLPDDVAEAARNGKRLMLYFGQEGCPYCAALF
                     NTNFSQPHIVEYTQKHFDAIELNIWGDREVTDFSGNTLPEKEFAAKLKVRFTPTLLFF
                     NEKGEQVLRINGYYPPHQFMAALRYVAEKQEGRMTFREYLAKQVPAPAKGVLQSQPFF
                     AKPPHDLSKISSGKPVAVFFEQKDCAGCDRMHAEMAGNAATLELLKRFHVVQLDRWSA
                     TTLVTPAGKKISARQWADELGIAYVPTAVLYDAGKEVIRIEAFMKGFHVQSVLDYVAS
                     GAYKKQPELQRFIRERADHLREQGVVVDIWK"
BASE COUNT         3324 a         5005 c         4803 g         3078 t
ORIGIN      
        1 caaattcctt cgtcgcccgg acgcgaacag cgccgccgcc cctggcggac tccaccttgt
       61 ccgccagcga gaacaatctc gccataccct cctcgccttt caggcgccag gtctcttccg
      121 cgtccacggc gaaggaaaac ccgtgcacca gccaggtgac gacgggcccg tccttcgatg
      181 cgcggcgcgc ggacttccgg atcgccggca gggaacgctc catcacggac accacgtcct
      241 gcagcgcctc ggaggtcttt tcctccggca ccagccagta gaacacagtg gcgtcgcgaa
      301 agaaggcatg caacagcggc atgcccttga aaaacggcga ggtgacgagg ccgtagcgcg
      361 acaggccgac ctgtttcttg agcgaggcac caagtgcaag acgggcgcga tggttcttga
      421 ggtggacatg gcactgcacc agcaccacgc ggcaggcatt gccttcatcg agcgccgtgg
      481 aaaagaacgg ctggccgctg cgacggaaac cggaaaacat gctgttgaaa cgcgccacgg
      541 cgaagtacgc cagactgaag gtgaacggcg cggtgagatc gacgaagatc gtggaaaagt
      601 tgagcgtgag atacgacact gcaaggaatc cggattgcag gacggtgaag gtcaggttca
      661 ccacgcgctg gtcgatgttg tacacgaatg cgatggtcag cagcacgacc gataccagcg
      721 tgaccagaat ataaatccac ggcggcagtt cggtgaggaa gtcgcccttt ttcaggttgt
      781 cgatggcgga cgccaggatt tccacgcctg ggtgcgcctt cgctaccggg gtagtcttca
      841 ggtcgaacag tgacggcgcc gtggaaccga taatgacgat cttgccggcg aactcgtccg
      901 cggggcgtgt ctttttgctc ttcagcagat cgaagtaaat ctcgtggaag gacacgcgcc
      961 ggtaggacgg cggcttgccg cgccagttaa gcagcacatc ggtctggtcc ggtaacgaca
     1021 ctcccagagc cgataccacg ttggccggca acgaacccac gcgccagccg tactcgttgg
     1081 catacacctc atagttccgg accatgccgt cttcatcggt gtagatgttg ttagtgccga
     1141 ggcgatggtc ggtcagcgca tcgaaaaaga atggcaggat cgctgcgatg gtggcggttt
     1201 cggatgccgc cagatcgagt ttcgagacgc cgggcagctg catcacgcgt aattcgctcc
     1261 gtgaatcatt ttcttcgttc aagcggatca tcgggaaaaa cgtgttgggc gtggcggcaa
     1321 tggcgctacg aaaatattta tcgccctcga cgttgaatac gtcgggatcg ctgaaggcga
     1381 tatcgaacac gatggctttc ggctgttgcg ccgcgatgcc ttccaccagc tcgcccatga
     1441 tgttgcgcgg ccatggccag cggccgtatt ccggcgccat ggccgcgagc gtggactcgt
     1501 cgatgtccac gatcacgatg tccgcatcag ccgcgggatg ctggaagcgc gtcttcatga
     1561 tcaggtcgta ggccttgttc ttcatgccgg cggtcgatcc ggtggcaatc gcgaatgtcg
     1621 ccgccgcggc gaccacgacg gcgaggccga cgtaaaacca gtttcccaac cggtcggaaa
     1681 gcgaggacag cggcagcagg aagaaacgcg cgacgcgctg gagagaattg cgaacagcca
     1741 tgcggcacct tccttagttt tcgggattat cttacgaata ccggcttgaa atcattctaa
     1801 acctgcttta ccctagcgcc ccacgaaagc agtcaacaat tttaagccca ttcagaaaga
     1861 gaggaagagc ccatgaaaac tccgctggat tctattatcg gcacactcat cagcggtctg
     1921 atcctgaccg tgattttgta tttcttcgtc gtcaatttcc tggtcaagac cgtcgtctga
     1981 cctgatcacc aaacgaacaa ctcacgggag aacaacatgg aactgactat cgtccgctgg
     2041 ttgcacatct tcgccggtat tacctggatc ggccttcttt attatttcaa tttcgtccaa
     2101 gccgtggccc tgcccagggc caaggccgat aacaccgccg ccggcatcac caagcacgtc
     2161 gcgccgcagg cactgctgtg gtttcgttgg gctgctgtgg cgacctggat cctcggcgcc
     2221 tattacctcg agcgctccgg cgtcggcctc ggcaacgcct tcctgctcaa gggttcggcc
     2281 gccgccatcg gcgtcggcgc ctggctcggc acgattatgc tcttcaacgt ctgggtgctg
     2341 atctggccca accagaagaa ggtgctgggc ctggtacagg ctgatgacgc cgccaaggcc
     2401 aaggccgcgc gcgtcgcctt cctgacctcg cgcaccaata ccatgctgtc cctcccgatg
     2461 ctgttcttca tggtcgcggg cagcgtgggc cacggcttgc cgcacatgta aaaaaacagc
     2521 gccatcgctg accaaggccc ggccatgccg ggccttgtgc tttctggctc ttgcgttatt
     2581 gtccgcaggg cgtagaattt ttgctttaag tggcgcccgc aaggcgcctt tgtcttttta
     2641 gagcgcacaa aaaaatgtct tcctatcaga tgccgacctt cgccccgctg cctgtggcct
     2701 tcacgcatgg tgagggcgct tggctgtggg acacaaaagg caaaaaatat ctcgatgcgc
     2761 tcgccggcat cgccgtgtgc gggctcgggc acgcgcaccc ggccgtcaag gccgcgctgt
     2821 gcgatcaggc cggcaaactg ctgcacacct cgaactggta tgaaatcccc ttgcaggaac
     2881 aattggccga acgcctgtgc aaactggcgg gcatggacaa cgctttcttc tgcaattcgg
     2941 gcgcagaagc taacgagacc gcgatcaaac tggcgcgact ctacggccat cagcgcgaca
     3001 tcctgacgcc cggcatcatc gtcaccgagg gcagcttcca cgggcgcacg ctcgcgacgc
     3061 tttccgccac cggcaaccgc aagatccagg ccgggttcga gccgttggtg cagggtttct
     3121 atcgcgtgcc ttacaacgac ctcgccgccg tgcagcaggt cggtgagcgt aacaaagacg
     3181 tcgtcgcggt gctggtcgag cccatcaccg gcgaaggcgg cgtcagcatt ccggacacgg
     3241 gttatctcaa gggcttgcgc aaaatctgcg acgagcgcgg ctggctgctg atgctggatg
     3301 aaatccagac cggcatgtgc cgcaccggca aatggttcgc ctgccagcac gagggcgtgg
     3361 taccggatgt catgacgctg gccaaggggc ttggcaatgg cgtgccgatc ggcgcctgtc
     3421 tcgcgcgcgg caaggcggcc caagtgttca agcccggcag tcacggttcg accttcagcg
     3481 gcaacccgct ggtgtgccgc gtggcgctcg ccgtcatcga cgagctggag aaaggcaaat
     3541 tcgccgagcg cgccgccaaa ctgggtgaac gcctgctcgc cagactcaag gaaatactgg
     3601 gcaaggtcgc gggcgtcaag gaaatccgcg ggcgcgggct tatgctcgcc atcgaactcg
     3661 accggccgtg caaggaactc ttgcaacggg cgctggaaag cgggattctg atcaacgtca
     3721 cggcggaaaa cgtcatacgc ctgctcccgc cgctcatctt caccgacaac gaaaccgata
     3781 tgctggtcga caaactcgac gaggtagtgc gcgcctttct cagagggccg acatgagcag
     3841 ggagccggga ccccgcgcga agcgcgggga tgcgaccggc cgctcatggc gccagaggcc
     3901 gacaacgggc gtaaccggcc tgatgtcgat ttccggaggc cgagaatgaa accgcgtcat
     3961 ttcctgacat tgccggacct gactcccgcg gaactcaagc gggtgctggc gcgcgccgtg
     4021 gagatgaagg ccatgctgag gcgcggcgag cgacacatgc ccctcgccgg aaaaaccatg
     4081 gcgatgatct tcgaaaaatc ctccacgcgc acgcgcgtgt cgttccaggt cggcatggcg
     4141 caattgggcg gcacgagcct gtttctctcg ccgcgcgaca cccatctcga ccgcggcgag
     4201 ccggtggagg acaccgcgcg cgtgctgtcg cgcatggtgg acatcatcgt gatccgcgcc
     4261 aattcgcata ccatggtcga acgtttcgcg gcgcactcgc aggtgccggt catcaatgcc
     4321 ttgaccgatc tcgaccaccc gtgtcagctg ctcgccgatg tccagacctg gatcgaaaaa
     4381 cggggcgaca tcgccggcag gaaggtcgcc tggatcggcg acggcaacaa tgtctgtcat
     4441 tcctggatca gcgccgcgcg tctgctcggt ttccatctcg atgtcgccac gccgaaaggc
     4501 tacgaaccga aatctgcgat cgtcgtggcc agcaaaaacc atgtcacgtt gcaccacgac
     4561 ccgaaacagg ccgcgcgcgg cgcggatatc gtcgtcaccg atacctgggc cagcatgggg
     4621 caggaacatg aaaaggcgga gcgcatcaag gccttcgccg gcttcatggt ggatggtgcg
     4681 ctcatgaaac tggcgaacaa ggacgccgtg ttcatgcact gcctcccggc ctatcgcggc
     4741 atggaagtgg ccgccgaggt catcgacgga ccgcagagcg tcgtttggga cgaggccgaa
     4801 aaccggctgc atgcccagaa ggccctgctc gaattcctgc tcgctggaaa acaggaataa
     4861 caggatttac aggattcaaa acagggttaa catgattgtt ctagaattta aaaaatcctg
     4921 tcaatcctga gaaatcctgt taatcctgtc tattttttgc cgtgaacgaa atactcgcgc
     4981 tcaacgacat cgaatgccgc tacgacgggc gcctcgcggt gtgcggtttg tcgatgcgcg
     5041 tcagcgcggg cagcatcgtc tgcctgctgg gaccgagcgg atgcggtaaa accacggtac
     5101 tgcgcgccat cgccggtttt cattccctga gcgcgggcga catctggatc aacggcagcg
     5161 ccgtttccca gccgggcttc accctgccgc cggaaaaacg ccatctcggc atggtatttc
     5221 aggaccacgc cctgtttccg catctcgacg ttgccggcaa catcggcttc ggcctgcgcg
     5281 gcatttcaga caaccagaaa ctggcgatca tcaatgaaat gctggaggtc accggcctga
     5341 acggcatggg cgaacgttac ccgcatgagc tttccggcgg gcagcagcaa cgcgtggcac
     5401 tggcgcgcgc gctggcgccg aagcccgatc tcatcctgct cgacgagccc ttctccaatc
     5461 tcgatattga actgcgcgag cgtctgtcag gcgaggtgcg cgagattctc aagcagcgcg
     5521 gcaccaccgg tgtgctggtg acgcacgatc agcacgaggc cttcgcgctc ggcgatgccg
     5581 tcggcgtgat gcacgagggc aagctgttgc agtgggacac gccctacaac ctgtaccacg
     5641 agccgagcaa ccgtttcgtc gccgacttca tcggccaggg tgtgttcctg cgcggcacgc
     5701 tgctcacacc cgacacgatg gaaaccgaaa tcggggtgca caagggcaat cgcgcgtatg
     5761 actggggacg gggagcgcag attgaactgc tgttgcggcc ggatgacatc gtgccggacc
     5821 cggaaggccc gctcaaggca aaggtggtcc aaaaggcctt ccgcggcgcg gacatacttt
     5881 ataccttgcg cctgcccagc ggcggtcgtc tgctcgcgct gtttccgagt cacgccgatc
     5941 acgccatcgg cgaactcgtc ggcatccgcg tgcaggccga tcacctcgtg gccttcccgc
     6001 tcgcggcggg gcattcagct ggcaccactc acgccacgaa ctagtcgcgt ctgatccgcc
     6061 tctcccgcgc ggacaggacc tccgactact tgtgaaacga tttcccgcta ccgagcgttg
     6121 gcgcggcagc atcgggcgac tgcgtcagcg gcagcacact ccctgaagtc gaggtccaga
     6181 gcgtgatcgg gtcatgacgc atctggttct gtcccgtctc agccttggcc tcgcgagcca
     6241 gataaccgtg ggccgcgaga tacgccagga tcttgtccgc cgcttcgttg gccgactgca
     6301 acgaggtatc gatgacgatc tcgggattgg ccggcgtctc gtagggatcg gacacaccgg
     6361 tgaattgctt gatcagcccg gcgcgcgccc gcgcgtacag tcccttgcgg tcgcgtgttt
     6421 cgcaggtagc gagcggcgtg gccacgtaaa tctcgatgaa atcgccatcc tcctcgacca
     6481 tcgcgcgcac ctgcgcgcgc gtcttggcgt agggcgcgat cggcgcgcag atggcgatgc
     6541 cgccgtggcg cgtgataagg ctcgccacgt aaccctgacg caacacgttg atgtcgcgat
     6601 gctcgcgcga gaagccaagc tcggaggaga tgttcttgcg cacgatgtcg ccgtcgagca
     6661 gcgtgaccgg acgcgcctcg tgttccttga gccgcgcata cagcacctgc gccagcgtcg
     6721 atttgcccgc gcctgacagg ccggtgaaga acagcgtgaa gccgtgacgc agggttttgc
     6781 gccactcctc cagcaccgcc ggataggaaa accagtgggc gatttcggca ttgcgttcgt
     6841 gcgaggtctc gcgaatctgc cctttcatgc gccggctggc gccacgcggc gaaagcaatt
     6901 tctccggctg gtcttcctcc agcaccagat tgcggaacgg caccacctcg atgccgatct
     6961 cctgcgcatg ccacgccacg gactcaagcc catggtattg cccgtacagg ggctcgccgg
     7021 tggcgccctc gccctgatcg tcgtaatcat gctccacgat gaaatgcgag caaccgaaat
     7081 tcttgtgcag aatcgcatgc cacatggcgg agcgcacgcc gctatgacgc ggcgacagtt
     7141 ccagcagatt cagctgcgcg acctgcgatg aataatgatc gagcacggtg cacagggcgc
     7201 gcgcgcgaga gaagtgatcg gcgtcgttca accccttgcg cccgacgacc gcctgaatca
     7261 gcaacacggc gtccttcaga taagcggtgc gcttggtgaa ttccacgtgc gcgcggtgca
     7321 tcagatgatg cgggtgatag ccgaccacgc gtgtgacgtt gcggcgcgcc agctgcgcgc
     7381 gcacttgtgc cggcgtctgg cgcagcgggg aaaaatcatg atgtactggc agctccacga
     7441 cttcgagctt gccggtgatg cagacatcgc cgttggcgtt gagcagctgg ttcgccgccg
     7501 cgtgcatgaa cgagcgcgta ccatagaggc attgcgattc cgcaccccgg tccggttgcc
     7561 agatttccga caccgtgatc acagccatga gcgttccctc gccgtcacgc aaagcaatcg
     7621 ccagacccgg tgaaatttgt tcggcgaatt tcgacgacgc atcgagtgtc accgggacgg
     7681 gccagagcct gccgtcggca agacgcatgc ccgcgcaaac ggcctcgtaa tccgcctgac
     7741 ccatgaagga cgtgagcggt gagaacacac ccgtcgccag catttcaaga tcggcaagat
     7801 gtcgtggaga aagatcgagc gaaaccagat cgcgcgagac cgccttgagc tcggcagccc
     7861 gctcgggact gaccaacaga ttgatcagtg ttccgccgta gggttgattc agttcccttg
     7921 aggttcccaa acgatgcgca ccgctcatgg tatgctcctt gtcgcttccc gcccttccac
     7981 acactaaaaa cggcacgccg gcgaacagac gtaccttcct gttgcagggc gattgcggca
     8041 tccatgcagc cctggacggt gaaaccaaac cctggttgga gccggttatc cggcgattac
     8101 atgcgcaacc ccgtcggagg agaagcctat acagggtttt ctgaatgaca atcaggcggt
     8161 tagtcccggc gatggaagga cggttttgag tgaccgggag caaacggcct tgcccgtaag
     8221 atagttacat cgggaatgat ttttgctgcc atactcaaat ctcggcttgt ctgcatttcc
     8281 catgttttta cagcctgaaa acgggtttac aggggtgttt tgctaaaata gtcttgagaa
     8341 agagggattt gtggaaaaaa agatattacg cctgctgatt gttgacgatt ctccggatga
     8401 tgccgaactt gccgtggcct cgctgcgcaa ggccggtttc atgctcaaga atcagcgcgt
     8461 acaggacctc gcctccatgc aggtggcgct cgacaaggga acctgggacg tggtgctgtc
     8521 ggaatacacg ctcccgcatt tcggcgcgca actggcgctg gatctggtca agcgcgcgca
     8581 cctcgacatt cccttcctcg tcgtcacccg caacatccgt gacccggacc tggtcaagat
     8641 catgcacaac ggcgcgcacg acgtagtgct gaaaagccag tcggcgcggc tggcgcccgc
     8701 cgtcgagcgt gagttgcacg tggccgagga acgccgcaag tatcaaaaga tgtccttggc
     8761 attgaacgag gtcgagaaca agaaccgcgc catcatcgaa ggctcgcgcg aggccatctg
     8821 ttactgccag gacggcatgc acatcgacgc caacaaggcc tacctcgaaa tgttcggcta
     8881 ccaggacctg agcgaactcg agggcgtgcc cgtgctcaat ctcgtcgtca aatcggatca
     8941 cgtccggttc aaggattatc tgcgcaagat caattccccg agtcctcccg agccccagga
     9001 attcctggcg gcgaaaaagg acggacagca ttttcacgcg gaactggcga tttcgcccat
     9061 cgccctcaac ggtgaaagct gcacgcaatt cgtcgtcatc gacgtttcga aacgcaaggc
     9121 cgtcgagaat cgcctgcaat acctgaatca gcacgacccg ctcaccggac tgtacaaccg
     9181 tcatcacttt acccaggagc tgaccaaggc gctggagaag gtcaagcagg acggcggcca
     9241 gagcgccctg ctttatctcg atctccacga gctcaaggaa atcaacgaca cactcggtca
     9301 cgccgccggc gaccggctgc tgctcaagat cgcgcgcctg ttccgcgaaa aacttggtga
     9361 atcgacaatc ctcgcgcgtt tcagcggcga cgaattcacc gcgctgctgc acggcatgag
     9421 cgaggcccag atcaaggagg ccgtggccgc gttgctctca gcactcaagg aaaccacctt
     9481 cagcgagggc ggcaaaactt tccgctgcga cggcagcatt ggcgtggtgg tgatcgacaa
     9541 gaacagcgaa aatgcgcagg cgctgttgaa caaggcctac cagacctgcc tgggtaaccg
     9601 gccgtccaaa ccggcgcccg tggccgcgcc tgctccggcg ccggaaaccg ccccggtttc
     9661 aagatcggcg cccgcggccg aggctcccgc cgtggcggag gtgccggcac tggccccgat
     9721 accgatacct acggtgacgg aaatccgtcg tcccgcgcca gccgtctcga cggtcggtgc
     9781 gcacgattgg gaggcgcggc tcaaaaccgc gttggaaaaa gatggtttcc agctcgccta
     9841 ccagcccacg gttaatcttc acggcgaccc cgaggaatat ttcgaggtgt tgctgcggct
     9901 tgccggccgc aacggcgaac tgatccacgc cggtgaattc atgtccgccg cggaacaaag
     9961 cggtctgatg ggcgcgatcg accactgggt catccgccag tcggttgaat cgctcgccgc
    10021 gctgcatcgc gagggcagaa aggccagctt cttcgtcaac ctgtgcccgg cgtcgttgaa
    10081 agaccctgag ttgctgtcga tgctccggaa gcaggttgtc gctgcgcgca tcaagccgca
    10141 gtacctcgtg ctcgaggccg acgagcccgc cgtcatggcg cacccgtccg acgccgccat
    10201 gttcatgcaa gccgtgaaga aaatcggctg ccgcttcgcg atcgacaatt tcggcaacaa
    10261 cctgagcacg ctaaaccgcc tgcgcgacat gcccgtcgat ttcctcaaga tatcgggctc
    10321 gctgattcgc aatctctcga ccgacgtact cacctacacg tcgctcaagg ccatcatcga
    10381 tctggccaaa tccatgaaca agcagaccat cgccaaattc gtcgagaaag ccgacgatct
    10441 cggggtgctc tggaatctcg gtttcgatta cgtgcagggc aactactttc agcaggcgga
    10501 cgcccacacc gattacactt tcgcggacga gaccacgctc gccaccgaca ccagcacgcc
    10561 cacttgggcg aattcccgcc gctgagttct ccgtcgtatt tccgtgtcgg cccgggtcag
    10621 ggcgcgattc gccgttgcct ccgtccgtat caaccctata ataagcgccc tttgatccac
    10681 atcaggtagc tccatgtccg gccagttcat ttatacgatg catcgtgtca gcaagatcgt
    10741 gccgccgaat cgcgcgattc tgaaggatat ttcgctgtcc ttctttcccg gcgccaagat
    10801 cggggtgctg ggcctgaacg gttcgggcaa atccacgctg ctgcgcatta tggccggcga
    10861 gctccaggag tacgacggtg aggcgcgtcc gcaaccgggc atccatgtcg gttttctgcc
    10921 tcaggaaccg cggctcgatc cgaaaaaaga cgtgcgcggc aacgtcgaag aggcggtggc
    10981 cgggaccaag aaactgctcg acgatttcaa cgaaatctcc atgaaattcg ccgaacccat
    11041 gagcgatgac gagatgaaca aactcatgga aaaacaggcc aagcttcagg aaaaaatcga
    11101 cgccgccggc gcctgggacc tggaccgcaa gctcgaaatc gcggccgatg ccctgcgcct
    11161 gccgccgtgg gacgcggacg tgaccaaact atcgggcggc gaacgccggc gcgtggcgct
    11221 gtgccgcctg ctgctgtcgc aaccggacat gctgctgcta gacgagccca ccaaccatct
    11281 cgacgccgaa tccgtcgcct ggctcgaacg cttcctgcac gattaccccg gcaccgtcgt
    11341 ggccgtgact catgaccgct attttctcga caacgtcgcc ggctggattc tcgaactcga
    11401 tcgcggcgag ggcattccct ggaaagggaa ttattcctcg tggctggagc agaaggaaaa
    11461 acgtcttgaa gtcgaggaga agcaggaatc cgcgcgccag cgcagcttga aacaggaact
    11521 cgaatgggtg cgcaccaacc ccaagggacg gcatgccaag agcaaggcgc gcctcgcggc
    11581 ctacgaggaa ctcgcctcgc aggaattcca gaaacgcaac gagaccaagg aaattttcat
    11641 cccgcccggc ccgcgtctgg gtgaagtcgt ggtgcaggcg gaaaatctga gcaagggctt
    11701 tggtgaccgg ctgctgatcg acaagctgag tttcaacctg ccgcgcggcg gcatcgtcgg
    11761 catcatcggt ccgaacggcg ccggcaagac cacgctgttc cgcatgatca ccggccagga
    11821 gaaacccgac ggcggcaagt tgcgcgttgg cgaaaccgtg cagcttgctt acgtggacca
    11881 gagccgcgat acgctcgacg ggaaaaagac cgtgtgggag gaaatctccg gcggccatga
    11941 catcatgatc gtcggcaaga gcgaggttca atcgcgcgcc tactgcggtc gcttcaattt
    12001 tcgcggcgcg gaccagcagt aactggtgaa ggacctgtcc ggcggtgagc gcaaccgcgt
    12061 gcacctggcg aaactcctga aaagcggcgg caacctgctg ctgctcgacg aaccgacgaa
    12121 cgatctggac gtggacacgt tacgcgcgct cgaagaagcg ctgctcgact tcgccggctg
    12181 cgcggtgatc atctcgcacg atcgctggtt cctcgaccgc atcgccacgc atattcttgc
    12241 attcgaaggc gagagcaacg ccgtatggtt cgagggcaac tacgccgatt acatggcgga
    12301 caatgaacgc cgcctgggcg cggaagccgt gcaaccgcac cgcatcaagt acaagaaact
    12361 catccactga cctgccctcc ggggtccccg gccatcgccg gtttccggcg gcttctcgcg
    12421 gtttcggccg gaatcagggt ctaaatgggc cgattggtcg gaccggtggc tgggcggttg
    12481 cccccggcat ctgttagatt gttcccgccc tgaatgcatc aggaacttgc acagggaagc
    12541 tgtgtcatac acgttcggtt gatccttggt cgaggcattg aacaacgttg ccctgctgag
    12601 gctccttcca aacgggatta accgaacgca acaaacctta aacaacggag acaatcatga
    12661 tgaacgccaa gaaactcacc ggccttgctc tcgcaacggc cgccgccggc ctgttcgcga
    12721 ctgccgggat tcccgccgcg aacgccgcca aacatgaagg agaagccaag gtccattgcg
    12781 acggcgtgaa ctcctgcaag ggcacatcgg cctgccagac cgccagcaat gcctgcaagg
    12841 gccaaaacag ctgcaagggc aagggctggc tgtacatgac caaggcggaa tgcgatgcgg
    12901 cccaggccaa gctgaagagt gagatgcata aaaagtaagc tgaaatcctg gcacaggtaa
    12961 aaccgggtgc cgagaggcgc ccggtgtttt tcctccgccg atgacaagcg cccctgctct
    13021 tcctgaacgt ccttggctcg gcttcgggct gggcctgcgc cccgcgcatt atgaaaccgt
    13081 tctgaacgaa tcgctggccg tcgactggtt cgagatcatt tccgagaact atatcgtccc
    13141 gggcggcaag ccgctgcact acctcgaccg catccgcgag cgatacccca tggtcatgca
    13201 tggggtatcg ctttcaatcg gctcgcagga tccgctcaac cgcgaatatc tcaagcagtt
    13261 gcgcgccctc gctaagcgca tcgagcccca atggatttcc gatcatctat gctggaccgg
    13321 cgcgcatggc ctcaatgccc atgacctgtt gcccttgcct tataccgagg aagcgctgaa
    13381 acatgtggtg agacgcgtta gcgaggtaca ggattttctc ggtcgccgga tactgctgga
    13441 gaccgtctcc agctacgtca gttatccgga atcggaaatg agcgaatggg aattcctgcg
    13501 cgagattgcc acgcgcgccg attgcttgat cctgctcgac atcaacaata tctacgtcag
    13561 cagcatcaat cacgaattca acccgcacca ttatcttgac gccattcccg tggaacgggt
    13621 atggcagttc catctcgccg gtcatcgcaa tctcggcgat tacatcatcg acacccacga
    13681 tgagccggtg atcgatccgg tgtgggagct gtatgcacgg gccgtgcgcc gtttcgggcg
    13741 tgtctcaacc atgatcgagc gcgacgacaa tatcccgcca ctgccggaat tgctggcgga
    13801 gctggatcat gcgcgtcata tcgcggcctc ggtccggcaa gcgaccgccg ccacggccgc
    13861 ctcagcatga gcacgcttgc ccacctgcaa tccgagtttc agtcgttcct gcttgacggc
    13921 aatgaacgca tgcttaaccg cgtggccggc accgccaaag tcagcgccga acaacgcctc
    13981 gccatttatt acgacgccta ccggctgcga ctgctggagg cgcttacatc caattacccc
    14041 gtcttgcgcg cctggatcgg cgacgatgaa ttcgaaaagg ccgggattgc ctatctcgcg
    14101 gcccatcctt ccaggcattt ttccattcgt tggttcgggc accacctgcc ggaatttctc
    14161 ggcgccacgg aaacctggcg cgacagtccc tttctcgctg aaatggcttc gctggaatgg
    14221 accctgtccg aaatcttcga cgcggaggac agcgcggtca tgagtgtcga ggatatggca
    14281 cacattccag tgaaggcttg gccagacatg cgcctccatt tccatccggc cgtgcgccgc
    14341 ctggacctgc gctggaacgt gccactgatc tggaaggcca tcaaccaaaa tctggatgtc
    14401 gaaagaaata ccgtctcttg tcggggcgag accagtgcca cgccttctgc gggaaatgca
    14461 cgacatgccg aagcgcagca cgacattccg gcgccggaag caggcgaaca tctgcaagct
    14521 tggcttgtct ggcgccagga tctcaaaaca tggtttcgtt ccctgtccgt ggacgaggct
    14581 tgggcgctcg atgctgcccg cggaggtgaa agcttcgccg caatttgcga aggactgtgt
    14641 gagtggatcg acgcccggaa cgtggcgctg cacgccgccg ggctgatgaa acaatggatc
    14701 accgacggcc tcgtcagtga aattgattcc agtaaacaat aaaatacccg tcttggcata
    14761 acagacagcg acaataacaa catgtcacag cgaatgattt tcttcggggg cttcctgatc
    14821 tgtgccggtc tgatggcggc ggcgctgtac tttcagtacg gcatgaaact cgagccctgc
    14881 ccactctgca ttttccagcg gctgtttgtg attggcgtgg ggctgatact gctggccggc
    14941 gcgttgcaca atccggacaa gctcgggcgt cgcatctacg gcggactcac ggcgctgatg
    15001 gcggccctgg gcgccggtgt cgccggccgt catgtctgga tcgagaatct gccgttcgat
    15061 caacaacccg gctgcggcta cagtctcaac gacatgctgg aaacctttcc gctgctcaag
    15121 accctcaagc tcgtgctggc cggcagcggc ggttgcggtc agatcacctg gtcctttctg
    15181 ggactcagca tggcaggctg gacgctgatt ttctttatcg gcttcaccgt gctcgggctg
    15241 atgctggtat ttacttccct cggcaaacgc tgaaaccgtt ttaaatcatt tccagatatc
    15301 caccaccacg ccctgctcgc gcagatggtc ggcgcgctcg cggatgaagc gttgcagctc
    15361 gggctgtttt ttatacgcgc cggaggcgac gtaatccagc accgactgga cgtgaaagcc
    15421 tttcatgaag gcctcgatcc gtatgacttc cttgccggcg tcgtacaaca ccgccgtggg
    15481 cacataggcg atgcccagtt catcggccca ttggcgcgcg ctgatttttt tgccggcggg
    15541 agtcacgagt gtcgtcgcgc tccagcggtc cagttggacc acatggaatc ttttgagcag
    15601 ctccagcgtg gcggcattgc ccgccatctc cgcgtgcatg cggtcacagc cggcgcagtc
    15661 cttttgctcg aaaaacacgg cgaccggttt tcccgaagaa atcttgctca ggtcatgcgg
    15721 cggcttggcg aaaaacggct gtgattgcaa cacacccttt gccggtgctg gcacctgttt
    15781 cgcgagatat tcacgaaaag tcatgcgacc ttcctgcttt tcagcgacgt atcgcagcgc
    15841 cgccatgaac tggtgcggcg ggtaatagcc gttgatgcgc aacacctgtt cgcccttttc
    15901 attgaagaac agcagcgtcg gcgtgaaccg gaccttgagc ttcgcggcga attccttctc
    15961 cggcagggtg ttgccggaaa aatcggtgac ctcgcgatcg ccccagatat tgagttcgat
    16021 ggcatcgaag tgcttttgcg tgtattcgac gatgtgcggc tggctgaagt tggtattgaa
    16081 gagcgcggcg caatacgggc agccttcctg cccgaaataa agcatcaggc gcttgccatt
    16141 tctggcggcc tcggcgacgt catccggcag atcgagaaag gagctcttga accaggcggg
    16201 aatctcgaac
//
LOCUS       DBNGZE010000079         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1538544, whole genome shotgun sequence.
ACCESSION   DBNGZE010000079 DBNGZE010000000
VERSION     DBNGZE010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1538544"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="AC16FV_01990"
     CDS             complement(<1..400)
                     /locus_tag="AC16FV_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR2848864.1"
                     /translation="MPELPEVETTRRGIEPHVVGRTVTRLMVRNPRLRWRVTPKLVRE
                     FSGQTIQSVARRGKYLLLTTTAGTAILHLGMSGSLRVVPCAQAADAHDHVDIVLDNGD
                     CLRLRDPRRFGALLWTRDDPQQHKLLRDLGP"
     gene            complement(639..1544)
                     /locus_tag="AC16FV_01995"
     CDS             complement(639..1544)
                     /locus_tag="AC16FV_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR2848865.1"
                     /translation="MPDTEKPRETAQDQPPPAGLRQRWLVNAALLVLIGLLAWLAIYR
                     AGQEKDVAGPPLTTLATDTISQLRIERPEHPAITLEKTGTEWALTAPVRARANPFNVE
                     SLLRILAAPGETRFAAVAQDLAKFGLDKPQSRVRYNNEEIAFGSLHPLNSRIYVLYKN
                     EVVLIPGHYLASAIYPYNNFIDSRLFEEQRKLTAINLPDFTLTQENGTWQKHPPDSKL
                     TSDRLNDFAAEWQNARALGVEKYSGKEILDRIEVTSIRDGKTEKLTLGILAYKPDFVL
                     YRQDENLEYHFTEETGKRLLNISQQ"
     gene            complement(1557..>1947)
                     /locus_tag="AC16FV_02000"
     CDS             complement(1557..>1947)
                     /locus_tag="AC16FV_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR2848866.1"
                     /translation="PSSWSETGPLNGPIRFDKGRDIRGPLNLAVALTRTLDNKREQRV
                     IVVGDGDFLANTYIPNGGNLELGMSLTNWLSQDDAFVNIPIKTARDRSLDLSRGGQVA
                     IVAVFLALLPVTLVSSGVWIWLRRRKR"
BASE COUNT          368 a          545 c          607 g          427 t
ORIGIN      
        1 ctggtccgag atcgcgcagc agtttgtgtt gttgcgggtc atcgcgcgtc cacagcagcg
       61 cgccgaagcg acgcgggtcg cgcaggcgca ggcagtcgcc gttgtcgagc acgatgtcca
      121 cgtggtcatg ggcatccgcc gcttgcgcac aaggcacgac gcgcagactg ccggacatgc
      181 cgagatgcag aatcgccgtg ccggcggtgg tggtgagtaa caaatatttt ccgcggcgcg
      241 cgaccgactg gatcgtctgt cccgaaaatt cccgcaccag tttcggcgtc acgcgccagc
      301 gcaggcgtgg gttgcgtacc atcagccgcg ttacggtacg gccgaccacg tgcggctcga
      361 tgccacggcg ggtggtttcg acttcgggca gttccggcat gatttaatga aaacccatat
      421 caaaatgaaa ttaaccgcca agacgccaag agcgccagga aaggcaaaat tggacaggat
      481 tcacgggatt aaaaacagga ttagcagggt ttaatatctt ccaaaatctt taatcctgta
      541 aatcctgaat aatcctgtta atcctgtcta tttttccgtt gggggctgtc agaccagtat
      601 tttatttttc tatccgcgtt tatctgcggt gacaaatatt attgctgaga tatgttcagc
      661 aggcgtttgc cggtttcttc agtgaagtgg tactccagat tttcatcctg ccggtacagc
      721 acaaaatccg gtttataggc gagtatgcca agcgtcagtt tttcggtttt gccgtcgcgt
      781 atcgaagtga cttcgatccg gtcgagaatc tccttgcccg aatatttctc gacgccgagg
      841 gcgcgcgcgt tctgccattc cgcggcgaaa tcgttgagcc ggtccgaggt cagcttggag
      901 tccggtggat gtttctgcca ggtgccgttt tcctgcgtga gcgtaaagtc gggcagattg
      961 atcgccgtga gcttgcgctg ctcttcgaac agccggctgt cgatgaaatt gttgtaagga
     1021 taaatcgcgc tggcgagata atggcccgga atcagcacca cctcgttctt gtacagaaca
     1081 taaatacggc tgttgagcgg gtgcagcgaa ccaaaggcga tttcctcgtt gttgtagcgc
     1141 acgcgcgact gcggtttgtc cagaccgaac ttggcgaggt cttgcgcgac ggcggcaaag
     1201 cgggtctcgc ccggcgccgc gaggatgcgc agcaggcttt cgacattaaa cgggttggcg
     1261 cgcgcccgca ccggcgccgt gagcgcccat tccgtgccgg ttttttccag cgtaatggcc
     1321 ggatgctcgg gccgttcgat gcgcagctgt gaaatcgtgt cggttgccag cgttgtcagc
     1381 ggcggtccgg cgacgtcttt ttcctggccg gcgcgataga ttgcaagcca cgcgagcagt
     1441 ccgatcagca cgagcagggc ggcgttgacc aaccagcgct gccgcaggcc cgcgggaggc
     1501 ggctgatcct gtgcggtttc gcggggtttt tcggtgtcgg gcatccgtgc ctgcctttag
     1561 cgtttgcgtc gccgcagcca gatccagacg ccgctgctga ccagcgtgac cggcaacagc
     1621 gccagaaaca ccgcaacgat ggcgacctgg ccgccgcgtg acagatcgag cgagcggtcg
     1681 cgcgcggtct tgatcggaat attcacgaag gcatcgtctt gcgacagcca gttggtcagg
     1741 ctcatgccga gctcgagatt gccgccgttg ggaatatagg tgttcgccag aaagtccccg
     1801 tcaccgacga cgatgacgcg ctgttcgcgc ttattatcga gtgtgcgcgt cagcgccacc
     1861 gccaggttga gcgggccgcg gatgtcccgg cccttgtcga accggatcgg cccgttgagc
     1921 ggaccggttt cggaccagct cgaaggg
//
LOCUS       DBNGZE010000080         2291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1330484, whole genome shotgun sequence.
ACCESSION   DBNGZE010000080 DBNGZE010000000
VERSION     DBNGZE010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1330484"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16FV_02005"
     CDS             <1..380
                     /locus_tag="AC16FV_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI/CycH"
                     /protein_id="HIR2848867.1"
                     /translation="TALKVTVSISPKLAKKAAPNDTVFIFARATNGPRMPLAIVRKQV
                     KDLPATVVLDDSQGMNPQMTLSSVPEVTVIARVSKSGMADARDGDLEGVSTPVTSGTK
                     TISISIDKVLTGQKAPMAPHGYR"
     gene            389..1081
                     /locus_tag="AC16FV_02010"
     CDS             389..1081
                     /locus_tag="AC16FV_02010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR2848868.1"
                     /translation="MFEYQLRESARARHVRLRVTPQSGLEVVVPRGYNTAHIPGMLEQ
                     KQAWIRAALERARLSEPETIWKLPDAIEFPALNQAWQVAAQEADMPWAAVRETGPGRL
                     LIHGRITDEQSSRAALARWLTRQACTHLVPRLEELSRQLGLAFRHVYIKRQRTRWGSC
                     SHHKAISLNAKLLFLDPMLVRYVMVHELCHLAEMNHSKRFWSLVQKHHADFRAHDREL
                     RKGWQSVPRWAG"
     gene            1214..1972
                     /locus_tag="AC16FV_02015"
     CDS             1214..1972
                     /locus_tag="AC16FV_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR2848869.1"
                     /translation="MLRALLAAPWRAQLIIIAIVAVALWAAVNWTYQVVRKPAELFFP
                     VSDTLFKTPFETWQQYEPIFQAHSTAVITPEFLAALAQIEGSGNPVARTYWRWQFTWD
                     LFELYRPASSAVGMYQITDSTFAEARRYCIHDHVVVEGGPWHELRSCWFNSLYTRVVP
                     SHAAELTSAYLDRHVAGTLERYRIASATLQQKQDLAAVIHLCGAGAGETYAKRGLRLT
                     NGQHCGDHDVRRYLARINAMKRVFSRLAASTRDE"
BASE COUNT          460 a          695 c          713 g          423 t
ORIGIN      
        1 cgacagccct gaaggtaacg gtatcgataa gcccgaagct ggccaagaag gcggcgccga
       61 atgacacggt atttatcttc gcgcgcgcca ccaacggacc gcgcatgccg ctggcgatcg
      121 tgcgcaagca ggtcaaagac ctgcccgcca ccgttgtcct ggacgacagt cagggtatga
      181 atccgcagat gacattgtcg agcgtgcccg aagtgacggt gattgcgcgc gtttcgaagt
      241 ccggcatggc cgatgcgcgc gacggcgatt tggaaggcgt gagcacgccc gtgacgagcg
      301 gcaccaagac catatcgatc agcatcgaca aggtgctgac cggtcaaaag gcgcccatgg
      361 cgccgcacgg ttaccgctga gcaaagcctt gttcgaatat cagttgcggg agagcgcgcg
      421 ggcgcggcac gtgcgtctgc gcgtcacgcc acagagcggt ctcgaagtgg tcgtgccccg
      481 gggttacaac acggcacaca tacccggcat gctggagcag aagcaggcct ggattcgcgc
      541 ggcgctggag cgcgccagac tgagcgaacc tgagactatc tggaaattgc cggacgcgat
      601 cgagttcccg gcgttgaacc aggcatggca agtcgccgcg caggaagcag acatgccctg
      661 ggccgcggtg cgtgaaaccg gtccgggtcg cctgttgatt cacggccgca tcacggacga
      721 acaatccagc cgcgccgcgc tcgcgcgctg gctcacgcgt caagcctgca cacatctggt
      781 accgcgcctc gaggaattga gccggcaact gggcctggcg ttccggcatg tctacatcaa
      841 gcgccaacgt acccgctggg ggagttgctc gcaccacaag gcgatcagtc tgaacgccaa
      901 gcttttgttt ctcgatccca tgttggttcg ttacgtaatg gttcacgagc tctgccacct
      961 ggcggaaatg aatcactcga aacgcttctg gtcactggtg cagaagcacc acgccgattt
     1021 ccgcgcgcac gacagggagt tgcggaaagg gtggcaaagc gtgccgcgct gggccggctg
     1081 accggcgaaa agagttggcg cctttatggc acgctcacgc accgcccgaa ccagttccgg
     1141 gaagaaaaga agaaagcgcc ggcgtcgttc acgcctcgcg tttttttggc caggcgcacg
     1201 actgcgccgg cgcatgcttc gggcgttgct ggccgcgcca tggagagccc agcttattat
     1261 tattgccatt gtcgctgtgg cgctctgggc cgcggtcaac tggacgtatc aggttgtccg
     1321 caaaccagcc gagctgttct ttcccgtgag cgacacactt ttcaaaacgc cgttcgagac
     1381 atggcagcaa tacgagccga tcttccaggc gcattccacc gccgtcatca cccccgagtt
     1441 tctggccgcg ctcgcccaga tcgagggctc gggcaatccg gtggctcgaa cgtattggcg
     1501 ctggcagttt acgtgggatc ttttcgagct gtaccggccg gcatcgagcg cggtcggcat
     1561 gtaccagatc actgactcga ccttcgccga ggcgcggcgc tactgcattc atgaccatgt
     1621 cgtggtcgag ggcggaccat ggcatgaact gagatcatgc tggttcaaca gtctttacac
     1681 gcgcgtcgtg ccgagccacg ctgccgagct gacgtcggct tatctggatc gccacgtggc
     1741 gggcacgctg gagcgctacc ggatcgccag cgctacgctc cagcagaagc aggacttggc
     1801 cgccgtgatc catttgtgtg gcgccggggc gggcgagact tacgccaagc gcggcctgcg
     1861 gttgaccaac gggcagcact gcggcgatca cgacgtacgt cgctatctcg cgcggatcaa
     1921 cgcgatgaag cgcgtgttca gccggctggc ggcttcgaca agagacgaat gaattaaagt
     1981 ttcttaaagt ctctaaggaa cctctgaaca agtccgttcg tggtgagccc ttcgacccgt
     2041 tcgtactgag cctgtcgaag tacgaacggg ctcaggacag gcctgtcgaa ccacgaacgg
     2101 acgaatcacc cgtcatttat ggcatttctc gttcaccctt cgacaagctc agggtgaacg
     2161 agagacttaa tcagaggctc cctaacaaaa tgacctccgc tccgcgcttg accctcaccc
     2221 caaccctgga agggggaggg agagggaggg ggcgcggcgt tcgtcatttt gttaggcgct
     2281 cttgatcaac c
//
LOCUS       DBNGZE010000081         1802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_913722, whole genome shotgun sequence.
ACCESSION   DBNGZE010000081 DBNGZE010000000
VERSION     DBNGZE010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_913722"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /gene="soxY"
                     /locus_tag="AC16FV_02020"
     CDS             <1..453
                     /gene="soxY"
                     /locus_tag="AC16FV_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR2848870.1"
                     /translation="RLFLKHTLATAVLVGVGASLFRPGWALAAEWPKDAFSAKKLDEA
                     ISSLFGASPVSPSPAIKLKVPTQAENSAQVQIQVLAEMPNVEAIAVFVEKNPSPLVAQ
                     AAFSGAEGYFSARMKMAQTSDVVAVVKAGGKLYSAKQNVKVTVGGCGG"
     gene            649..972
                     /gene="soxZ"
                     /locus_tag="AC16FV_02025"
     CDS             649..972
                     /gene="soxZ"
                     /locus_tag="AC16FV_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR2848871.1"
                     /translation="MADRKMKMRTRTQDGSVELQILIAHPMETGLRTDKNTGKKIPPH
                     FIQRVTLEHNGKLVATLNTGTGISEDPLIGFRLKGAKHGDKLKVFWTDNMGESGTLEG
                     TVDLS"
     gene            complement(1198..>1802)
                     /locus_tag="AC16FV_02030"
     CDS             complement(1198..>1802)
                     /locus_tag="AC16FV_02030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2848872.1"
                     /translation="RRLVVLFVLVLAVSAIKVSEDVLGGESGPIDEAILIFIRGHVPD
                     TLTGFFEAITFSGSSKVLFPLASVATIALLCAKRRFEALLLAASVIIGATVVYVVKAA
                     VGRARPALWETEWYWGSSFPSGHTLVVAAFATAAVVCVSRIWPAAREFALSIALLWIF
                     LVGFSRLVVGVHWPTDVLAAACIGAFLPLAMSVALELRHA"
BASE COUNT          423 a          543 c          530 g          306 t
ORIGIN      
        1 cgtctcttcc tcaagcacac gctcgccacc gccgtgctgg tcggtgtcgg cgccagcctg
       61 ttccgccccg gctgggccct ggccgccgaa tggcccaagg acgccttcag cgccaagaag
      121 ctcgacgagg ccattagctc gctgtttggc gcgtccccgg tcagcccgtc tcccgccatc
      181 aagctgaagg tcccgaccca ggccgaaaac agcgcccagg tccagatcca ggtcctggcc
      241 gagatgccca acgtcgaggc catcgccgtc ttcgtcgaga agaacccttc ccccctcgtg
      301 gcccaggccg ccttttccgg cgccgagggc tacttctcgg cccgcatgaa gatggcccag
      361 acctcggacg tggtcgccgt ggtcaaggcc gggggcaagc tctactccgc caagcagaac
      421 gtgaaggtga cggtgggcgg gtgcgggggt tgatgaatgc tgatgaaaac gcggacaatt
      481 ttgccaggag cggaaattgg acagcggagc gcagcgacgc tggcccgagc gtaggcgagg
      541 gcgagagcca gggacggcgc gagcatgaac gcagattttt tgaacgtttc ttatatatca
      601 gcgttaatct gcgttccaga gatcatattt ttaatgagga atgattaaat ggctgatcgc
      661 aaaatgaaga tgcgcacccg cacgcaagac ggaagcgtcg agctgcagat actgatcgcg
      721 cacccgatgg aaaccggcct gcgcaccgac aagaacaccg gcaagaaaat cccgccgcat
      781 ttcattcagc gggtgacgct ggaacacaac ggcaagctcg tggccacgtt gaataccggc
      841 accggcattt ccgaggaccc attgatcggc ttccggctca agggcgccaa gcacggtgac
      901 aagctcaagg tcttctggac cgacaacatg ggcgagtccg gtacgctgga aggcaccgtg
      961 gatttgtcct gaaattcact tgtttcaaat aaaaaaaccg cccgatgggc ggtttttttt
     1021 atagacatcc agaatccaat gacgaagcca gctaagtacc gggaggaggg actggtatcg
     1081 gccaagagcg gccagtagtt ttttcaaata gtagacgctc agcgttttgg tgaacggcgg
     1141 gccggcacgg cggaaaatga tggcacggcg cttacggccc gtccgttcca cctgaagtta
     1201 ggcgtgacgt aactcaagcg caacgctcat tgccagtggc agaaatgctc cgatgcacgc
     1261 ggcggccaat acgtccgttg gccagtgcac gccaaccacc aaacgtgaaa agccaacgag
     1321 aaagatccat aagagcgcta tcgacagcgc gaattcgcgc gccgcgggcc agatccggct
     1381 aacgcagacg acagcagcgg tggcgaatgc cgcgacaacc aaagtatggc cactgggaaa
     1441 gctggagccc caataccact ctgtttccca aagtgctggc cgagctcggc ctaccgccgc
     1501 cttgacgacg taaaccacgg tcgcaccgat gatcactgaa gctgcgagaa gcagcgcctc
     1561 gaaacggcgc ttcgcgcaca gcagcgcaat cgtcgcaacg ctcgcgagcg gaaatagaac
     1621 ctttgacgac ccgctaaacg tgattgcttc gaaaaagcca gtcagtgtat ccgggacgtg
     1681 accgcggatg aatatcagga tcgcctcgtc gatcggcccg gattctccac cgagtacatc
     1741 ttcgctcacc ttgatcgcgg acaccgcaag gacgagcacg aacagaacga ccaggcggcg
     1801 ct
//
LOCUS       DBNGZE010000082        14753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1608349, whole genome shotgun sequence.
ACCESSION   DBNGZE010000082 DBNGZE010000000
VERSION     DBNGZE010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 14753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14753
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1608349"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1145)
                     /gene="pstS"
                     /locus_tag="AC16FV_02035"
     CDS             complement(60..1145)
                     /gene="pstS"
                     /locus_tag="AC16FV_02035"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIR2848873.1"
                     /translation="MTIMRIFPTYLKHTVAAAAMVLGMAAGLVQAAETINGAGATFPY
                     PIYAKWAEAYKAKTGTSMNYQSIGSGGGIKQITARTVDFGASDAPLTVEKLSEIGLMQ
                     FPMVMGGVVPVVNLDGVKAGELKLTGTVLADIYLGKITNWDDAALKKLNPGVNLPNSK
                     ITVVSRADGSGTTFIFTHYLSKVSAGFKEKVGNNTSVAWPTGVSGKGNEGVASYVQRL
                     KGAIGYVEYAYALQNKMTFAQMQNKSGQFVKPDSETFQAAAAGADWKGTPGFNLMLTD
                     QQGAKSWPITGATFILVYKKQEKTDTAHQVLKFFAWAYHHGDKLAEQLDYVPMPASVV
                     KLVEETWKTEIKDANGKAVWTDSMVQK"
     gene            complement(1268..2347)
                     /gene="arsB"
                     /locus_tag="AC16FV_02040"
     CDS             complement(1268..2347)
                     /gene="arsB"
                     /locus_tag="AC16FV_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIR2848874.1"
                     /translation="MGLFERYLTLWVFLCIGAGILLGHLFPELFHAVGRLELARVNLP
                     VALLIWLMIIPMLLKVDFGALHHLRDHWRGIGVTLSINWLVKPFSMALLGWIFIRHVF
                     APWLPADQIDAYIAGLILLAAAPCTAMVFVWSNLSRGHANFTLTQVALNDTIMIFAFA
                     PIVGLLLGLSAITVPWNTLLLSVGLYIVIPVVLSQLWRGWLLRRGGQAALQSTLDRLH
                     PVSLIALLTTLVLLFGFQGEQILRAPLIIALLAVPILIQVYFNSGFAYLLNRRFRVAH
                     CVAGPSALIGASNFFELAVAAAISLFGFESGAALATVVGVLIEVPVMLSVVKIVNRSS
                     AWYERKPGVPTYAECCPAPVISSTY"
     gene            complement(2392..2850)
                     /gene="arsC"
                     /locus_tag="AC16FV_02045"
     CDS             complement(2392..2850)
                     /gene="arsC"
                     /locus_tag="AC16FV_02045"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HIR2848875.1"
                     /translation="MYKRKVKVLFLCTGNSCRSQMAEGWANHLGHEWLEAYSAGIEAH
                     GKNPRAIAVMREAGVDISHQESTRVTPEMLASADLVVTVCGHADEHCPVLPPGVQKKH
                     WPLEDPAKATGTEEQIMTRFRATRDEIKDRVEDLIGGLKMMARSDSKEEA"
     gene            complement(2870..3319)
                     /locus_tag="AC16FV_02050"
     CDS             complement(2870..3319)
                     /locus_tag="AC16FV_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848876.1"
                     /translation="MKAACCTRIMFVSMLVVLAPTTSQAAPDTRVAIELPAPMRAHML
                     ANMRDHLAALHEIQAALARDENQRAAEIAEQRLGMSSLDAHGAGHMAPYMPKPMQDIG
                     TAMHRAASRFARVAEEAGVRRGLRPALQALSDVTQQCAACHAAYRLK"
     gene            complement(3316..4662)
                     /locus_tag="AC16FV_02055"
     CDS             complement(3316..4662)
                     /locus_tag="AC16FV_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848877.1"
                     /translation="MTRTLPQAIPDLALQLNQTCHCITLDRVSLTQNIAVRGDDSATL
                     HASLEARPHLFSSTTVFVTPTQIAAMQEIIRAVENVVALPAWRDQVLSRAPEIAHIDP
                     GARGVFLGYDFHLGSDGAQLIEINTNAGGGLLNVLLSHAQLDCCQPLRKQTELPACQP
                     DLERVYLEMFLAEWRRSRGAQTLTRIAIVDDAPTEQYLYPEFQLFRALFERHNLEAVI
                     ADARELVWRNGALWHEEQRIDLVYNRLTDFYLAAPEHAALRAAYEASAVVLTPHPRAH
                     ALYADKRNLARLSDPDFLRTLDLPAATMETLARGIPRTVAVDAAHAEALWATRRRYFF
                     KPAAGYGSKAAYRGDKLTKRVWSEILGADYVAQETVPPSERTLHVNGDTQDLKLDVRV
                     YVYDGAIQTLAARLYQGQTTNFRTQGGGFAPVVMVPEDPPVHAARAQAATDCPEGA"
     gene            complement(4679..5029)
                     /locus_tag="AC16FV_02060"
     CDS             complement(4679..5029)
                     /locus_tag="AC16FV_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HIR2848878.1"
                     /translation="MITADEFFPALADATRLRCLLLLAGEAELCVCELTHALDESQPK
                     ISRHLATLREAGIVSDRREGLWIHYRINPELPAWAREILNTTSRANATAKPFAQDRKR
                     LRGAPNRPPVRCCD"
     gene            5115..5567
                     /locus_tag="AC16FV_02065"
     CDS             5115..5567
                     /locus_tag="AC16FV_02065"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR2848879.1"
                     /translation="MRYPIRVLFLCTGNSVRSQMAEGLLRSVGGGDFEVHSAGTDPKE
                     LHPLAVEAMRETGIDISGHENKSLERFLGQHFDYIITVCDRARDSCPTFPGDNQRIHW
                     RFEDPAAVTGSRAEQLAVFRRVRNEISERLRVWVTVQRKLLKEIGHPA"
     gene            complement(5581..6042)
                     /locus_tag="AC16FV_02070"
     CDS             complement(5581..6042)
                     /locus_tag="AC16FV_02070"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR2848880.1"
                     /translation="MPTARALVTNDNDLWLQIVDHFYPVLVRSLTGLAIVALCAWMGA
                     GVIVLVLDLRQVLTSGWASVAEHAIIDTLIMLALLEVIRTLQSYLKLGRVRVTFILDT
                     ALVVLIGELMGLWFREYAPEKVLLGLGVIMALVVLRIVTAHFSPERGGEPA"
     gene            complement(6327..7838)
                     /locus_tag="AC16FV_02075"
     CDS             complement(6327..7838)
                     /locus_tag="AC16FV_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR2848881.1"
                     /translation="MKHVPQLKPCVFAVAMTTIFTTFAAQADDDTDFGLKIEQLLKAQ
                     SEKLFGVEKPLEASASPTVGPYRTPLQTAYDQALVAHGLKVEYLTRDAANATDMMAFY
                     PLQNPTHLITCVEGGRQFLIDGGTPNVYDAGDKLNPSVQRINLNTGAVETILRGMTSC
                     DGIRTTAWGTTLATEETSNGGVYEIFNPLGVTEQTVIDRASGMVTDPANIAKRTALPT
                     IAWEGIAVLSNGVVIAGDELRPGTGGADRDGGAIFKFVPAMPRLVSGPITNLSESPLG
                     AGSVYAMQVSCVNNTQQYGQGCEIGNAAWVAVNATTARSDANAAGATRYYRPEDLHQD
                     PMYQDGLNPEAVRFCWTNTGNENGSNYAEVVCGVDRVPLTASNIQRTVVVNRFIEGDA
                     DFNSFDNLEFQPKTGNVYVIEDHDNGDIFACLPDGMDRDIKTDGCVKIMSVKDSSAEP
                     TGFLFSADGGTAYVSIQHSDDANMSTVDGYPTDDVLKITGFKLRGKKDGWQWW"
     gene            complement(7937..9259)
                     /gene="phoR"
                     /locus_tag="AC16FV_02080"
     CDS             complement(7937..9259)
                     /gene="phoR"
                     /locus_tag="AC16FV_02080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HIR2848882.1"
                     /translation="MPQTIWREIWILAGIAIASLVLGLITGRPFLIAAIGFGLYIFFT
                     MRHLQRLHYWLLNRQKAEIPDAEGLWGDVFNEIRQLMKQTEHREDQLNDMLTRFQSAA
                     AAMPDAMVILSQDGDIEWANPSAERLFGIRYPRDVGTRLFNLLRDPDFVQYLQRGDYS
                     ELLEINSPENSEIHVSIQVTPFGSLQKLMIGRDVTRLARLEQMRRHFVANVSHELRTP
                     LTVLGGFVETLGHMDQIRMEELKKHLATMQEQTLRMQRLVDDLLMLSKLETAPPRTHD
                     EKVDVISLLAGLKEQAELLSGEQHHVITIEADRNLRLLGSRDELLSAFSNLINNAVRY
                     TPAGGRIALRWGMAPDGGARFTVSDSGVGIAPEHVPHLTERFYRVDTARSRASGGTGL
                     GLSIVKHVLLRHDARLEIESEPGVGSSFTCYFPSTRTRHSDSSASGAA"
     gene            complement(9263..9952)
                     /gene="phoB"
                     /locus_tag="AC16FV_02085"
     CDS             complement(9263..9952)
                     /gene="phoB"
                     /locus_tag="AC16FV_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HIR2848883.1"
                     /translation="MRGKILIVDDEPAIRQMVCLALSQANYDCLEATDTTEAQTKILA
                     DKPDLILLDWMLPKVSGMDYARQLRREKLTEDIPVIMLTARTEEEDKVRGLDSGVDDY
                     ITKPFSTRELLARIRALLRRTSPHAAEVAVETGGLSLDPVTHRVTAADKTLSLGPTEF
                     RLLHFFMTHPERVHSRERLLNDVWGNNVYVEERTVDVHIRRLRKILTPTGHDRLIQTV
                     RGTGYRFSAQG"
     gene            10119..10499
                     /locus_tag="AC16FV_02090"
     CDS             10119..10499
                     /locus_tag="AC16FV_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR2848884.1"
                     /translation="MSQITVRPTDIKLHQKSRILEVAFDDGSHFRLPCEYLRVYSPSA
                     EVRGHSPGQEVLQVGKENVNITHIEPVGTYAICLYFDDGHSTGIYSWDWLHQLGKNQD
                     SLWKQYLERLAKAGHQRKPAKAAE"
     gene            10506..11267
                     /gene="ubiE"
                     /locus_tag="AC16FV_02095"
     CDS             10506..11267
                     /gene="ubiE"
                     /locus_tag="AC16FV_02095"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012551397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR2848885.1"
                     /translation="MSQPGHNERKTHFGFREVPESQKDGLVADVFHSVAGKYDLMNDL
                     MSLGAHRLWKQFATEASGVRGGFRVLDVAGGTGDMAAKFAPRVGRRGQVVIADINSSM
                     LEVGRGRLTDQGFVGNVDFVQANAENLPFPEGYFDRVSIAFGLRNVTHIDRALSSMFR
                     VLKPGGALLVLEFSRPAVPRLNRLYDAYSFNVLPWLGRVVAQDEGSYRYLAESIRKHP
                     DQETLKGMMERAGFERVQYFNLSGGIVALHKGYKF"
     gene            11379..11969
                     /locus_tag="AC16FV_02100"
     CDS             11379..11969
                     /locus_tag="AC16FV_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiJ"
                     /protein_id="HIR2848886.1"
                     /translation="MLLEEAINRALRLDPATLARLGELEGKVIRLRAAGDRPFEIFIL
                     PDESGLWLRGEYDREPDVTLTGDIPVIFRFALRRIIPDVVAAGEVQISGDIDLGQRFQ
                     RLLEQADIDWEEQAARVLGDVPAHQLGNALRDLGGWARRTLHTLKQDLSEYLQEESRL
                     LPVRPRAGAFRHSVETLEHDVESLEKRLTRLREMAR"
     gene            11966..13624
                     /gene="ubiB"
                     /locus_tag="AC16FV_02105"
     CDS             11966..13624
                     /gene="ubiB"
                     /locus_tag="AC16FV_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR2848887.1"
                     /translation="MKRLRQLGRLLRITQIFVRHDLDEFVTAIHLFRPYRLLLRLAPW
                     RLFARRNIPRGVRLREALEELGPIFVKFGQMLSTRPDLLPEDIASELARLQDRVPPFS
                     GDESVRLIEQALGGRLETWFSEFSREPIASASVAQVHIARLHDGAEVAVKVLRPGIES
                     VIERDLELLYLLARLAIRYAPEARRFRPVEVVDEFSKTIHDELDLRVESANASRLRAN
                     FEDSKLLHVPRVYWDLTRRTVMVLERIHGIPISNIEGLKAAGIDMRQLAHNGVEIFFT
                     QAFRDGFFHADMHPGNIFVSPEGQYRAVDFGIMGTLGEKDKRYLAENFLAFFNRDYHA
                     VADSHLRAGWVAPGTNLVEFEAAIRTVCEPVFARPIKEISFGRFLLHLFQTARRFNME
                     VQPQLVLLQKTLFNIEGLGRRLYPDLDLWETAKPYLERWMSEHMGPRAFLKSLRREFP
                     KWWAMLPEIPSLVHETLRRAGEADAAQESRAQELEQRLRQMRYSQRRLYFAIASSGLL
                     IAGAVLLGIDTVASEAWEWQRVTGWVLAASGVFMLLRGWPGRNP"
     gene            13694..14581
                     /locus_tag="AC16FV_02110"
     CDS             13694..14581
                     /locus_tag="AC16FV_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848888.1"
                     /translation="MKALNKAVLASAALLVAAGPALSAPTGKLSWFNDTPFAGVGALG
                     VPEARLLDQSDYTSFGINLYNSRYNSRQNAVEEGYPVAREITLDANTVRLYGEVESSR
                     SGAGSLSPVEDFPLGRVNAFGVSWQHRLNAMNSFAVSAEYGEGTAVYPLPVDTLDTRA
                     AFSWTSVLPSAVKPSITGSVFLGDEMAREEIYRHLGRRYYGFTVGGSLTLFQSHTPYV
                     SFKMQRSLYDTAEETLLISPRSGDRSLLSAGWKWQVQRDLSLQAEASYGLGEVPSSSL
                     DPYSLERSRILFGTRFGFK"
BASE COUNT         2793 a         4296 c         4612 g         3052 t
ORIGIN      
        1 taaagagaaa gcggggtcac gaaggacccc gcaggtttac agcttgtctg acggcaatct
       61 tacttctgga ccatgctgtc ggtccagaca gctttgccgt tcgcgtcctt gatctcggtt
      121 ttccatgttt cttcaaccag ttttacgacg ctggccggca tcggcacata atccagctgc
      181 tcggcgagct tgtcaccgtg gtgataagcc caggcgaaaa atttcaacac ttgatgggct
      241 gtatccgttt tctcctgttt cttgtaaacg aggatgaagg tggcgccggt gatcggccag
      301 cttttcgcgc cctgctgatc ggtgagcatc aggttgaagc ctggcgtgcc tttccagtcg
      361 gcgcccgcgg ccgcggcctg gaacgtttcg ctgtccggct tcacgaactg accggatttg
      421 ttctgcattt gcgcgaacgt catcttgttc tgcagcgcgt aagcatactc tacgtagccg
      481 atcgcgccct tcaggcgttg cacgtaggaa gccacgccct cgttgccctt gccgctgacg
      541 ccggtgggcc aggcgaccga ggtgttgttg ccgacctttt ccttgaagcc cgcgctgacc
      601 ttagacagat agtgcgtgaa gatgaaggtg gtgccggaac cgtcggcgcg cgacacgacg
      661 gtgattttgc tgttcggcag attcacgccc ggattgagtt tcttgagcgc cgcgtcatcc
      721 cagttcgtga tcttgccgag atagatgtcg gccagcaccg taccggtgag cttcagttcg
      781 cccgccttca cgccgtccag attcacgacc ggtaccacgc cgcccatgac catgggaaac
      841 tgcatcagcc cgatctcgct gagtttttcc accgtgagcg gcgcgtcgga ggcgccgaag
      901 tccaccgtcc tggccgtgat ctgcttgatg ccaccgcccg agccgatgga ctggtagttc
      961 atgctcgtgc cggtcttggc cttataggcc tcggcccatt tggcgtagat cggataagga
     1021 aaggtcgcgc ccgcgccatt aatggtttcg gcagcttgca cgagaccggc ggccatgccg
     1081 agcaccatcg ctgcagccgc cactgtgtgt ttcagatatg tggggaagat acgcattatt
     1141 gtcatgtctc cgtaatgtcg atagtcgttg aatgcggggc gcatgatgcc aaggcaatgt
     1201 gacagatgtg tgacaccgtt ttgccaggaa aaaccttggg ccaggtggtg tcaggagcgt
     1261 gctggtgtca atatgtggac gatatgaccg gtgccgggca acattccgcg taagtgggga
     1321 cacccggctt gcgttcgtac caggcgctgc tgcggttaac gatcttcacc accgagagca
     1381 tgaccggcac ttcgatcagc acgccgacga cggtggcgag tgccgcgccc gattcaaagc
     1441 cgaacaggct gatggcggca gccacggcga gctcgaagaa gttgctggcg ccgatcaggg
     1501 ccgagggccc ggcaacgcag tgtgcgaccc tgaaccggcg gttgagcagg taggcgaaac
     1561 cggaattgaa atacacctgg atcagaatcg gcaccgcgag caaagcgatg atcaacggcg
     1621 cgcgcagaat ctgctcaccc tgaaaaccga atagcaggac cagcgtggtc agtagcgcga
     1681 tcagagaaac cggatgcagc cggtcgaggg tactttgtaa tgccgcctgg ccgccccggc
     1741 gcagcaacca gccacgccac agctgactga gcaccaccgg gataacaatg tacagtccca
     1801 ccgacagcag cagcgtattc cacggcaccg tgatcgccga aagtccgagc agcagaccca
     1861 cgatcggcgc gaaggcgaag atcatgatgg tgtcgttgag ggcgacctgc gtgagggtga
     1921 agttggcgtg gccgcgactc aggttgctcc agacgaacac catggccgtg cacggcgccg
     1981 ccgccagcag gatcagtccc gcgatgtagg catcgatctg atcggccggc agccagggtg
     2041 caaacacgtg gcggatgaaa atccacccga gcagggccat ggaaaacggc ttgaccagcc
     2101 agtttatgga cagcgtcacg ccgatgccgc gccagtggtc gcgcagatgg tgcagcgcgc
     2161 caaagtccac cttgagcaac atcggaatga tcatcagcca gatcagcagc gcgaccggca
     2221 gattgacgcg cgccagttcg agccgcccga ccgcgtgaaa cagttccgga aacagatgcc
     2281 ccagcaggat tccggcgccg atgcacagaa acacccacaa tgttagataa cgctcgaaca
     2341 gccccatggg attacccgcg gcgcgtttgg cggtggcttc acattgcgcg ctcatgcttc
     2401 ctccttgctg tcgctgcgcg ccatcatttt caacccgccg atcaagtctt cgacgcgatc
     2461 cttgatctca tcgcgcgtgg cgcggaatct ggtcatgatc tgttcttccg ttccggtggc
     2521 cttggccgga tcttccagtg gccagtgttt tttctgtacg ccgggcggca gcaccggaca
     2581 gtgctcatcg gcatggccgc acacggtaac gacgagatcg gccgacgcca gcatttccgg
     2641 tgttacgcgc gtggattcct gatgcgagat atccacgccg gcctcgcgca tgacggcgat
     2701 ggcgcgcgga tttttcccgt gcgcttcaat gccggcggaa tacgcttcaa gccattcgtg
     2761 tcccaggtga tttgcccaac cctcggccat ttgcgaacgg caggaattgc cggtgcacag
     2821 gaagagaact tttactttgc gtttgtacat gcaatcccca tgaatgacat tacttcaacc
     2881 ggtaagcggc gtggcaggcg gcgcactgtt gcgtcacgtc cgacagggct tgcaacgccg
     2941 gacgcaggcc acggcgtacg cccgcctctt cggccacgcg cgcgaaacgg cttgcggcgc
     3001 ggtgcatcgc tgtaccgatg tcctgcatcg gcttcggcat gtagggggcc atatgtccgg
     3061 cgccatgcgc atcaagcgaa ctcatgccaa gccgttgttc cgcgatttcg gccgcgcgct
     3121 ggttctcgtc acgcgcgagc gcggcctgga tctcgtgcag tgccgcgaga tggtcgcgca
     3181 tgttcgcgag catatgcgcg cgcatcggcg ccggcagctc gatggccacg cgcgtatccg
     3241 gcgccgcctg agaggtggtt ggcgcaagca cgaccagcat ggaaacgaac attattcttg
     3301 tgcagcaagc tgctttcatg cgccctccgg gcagtctgtc gcagcctgtg cccgggctgc
     3361 gtggacaggc gggtcttcag gtaccatcac taccggcgcg aagccgccgc cctgggtacg
     3421 aaagttcgtg gtctggccct ggtacaggcg cgccgcgagc gtctggatcg cgccatcgta
     3481 gacataaacg cgtacgtcga gcttgagatc ctgcgtgtcg ccattgacat gcagcgtgcg
     3541 ctcgctcggc ggtacggttt cctgggccac atagtccgcg ccgagaatct ccgaccacac
     3601 gcgcttggtg agcttatcgc cgcggtaggc ggccttgctg ccgtaacccg ccgccggctt
     3661 gaagaaatag cgacggcgcg tggcccacag cgcttcggcg tgcgcggcat ccacggccac
     3721 agtacggggt atgccccgtg ccagcgtttc catcgtggcc gcgggcagat cgagcgtgcg
     3781 caggaagtcc gggtccgaga ggcgcgcgag gttacgcttg tcggcataca acgcatgcgc
     3841 gcgcggatgc ggcgtcagca ccaccgcact ggcttcatag gccgcgcgca aggcggcgtg
     3901 ttccggtgcc gcgaggtaaa aatccgtcag gcggttgtag accagatcga tgcgttgttc
     3961 ctcatgccac agagcgccat tgcgccagac gagttcacgc gcatctgcaa tcacggcttc
     4021 gaggttgtgg cgctcgaata acgcgcggaa cagctggaac tccggataca gatactgctc
     4081 tgtcggtgcg tcgtccacga tggcaatgcg cgtgagcgtc tgcgcgccgc ggctgcggcg
     4141 ccactcggcg agaaacatct ccagataaac ccgttccagg tccggctgac aggctggcag
     4201 ttccgtctgt ttacgcaatg gctggcagca atccagctgc gcatggctga gcaggacgtt
     4261 gagcaggccg ccaccggcat tggtgttgat ttcgatcagt tgtgcgccgt cggaaccgag
     4321 atgaaagtca taaccgagga acacgccgcg cgcgccggga tcgatgtgcg cgatctccgg
     4381 cgcacgcgaa agcacttgat cgcgccaggc gggcagggcc accacgtttt ccaccgcccg
     4441 gataatttcc tgcatggcag caatctgggt gggggtgacg aataccgtcg tagacgagaa
     4501 cagatgcggg cgtgcttcca gggacgcgtg caacgttgct gagtcatcac cccgtaccgc
     4561 gatattttgc gttaacgaca cgcgatcgag cgtgatgcaa tggcacgtct gattgagttg
     4621 caaggccaga tcagggatgg cttgaggcaa tgtgcgtgtc atgtgcccac accctgtttc
     4681 aatcacaaca acggaccggc gggcggttcg gcgcgccgcg cagacgcttg cggtcctggg
     4741 cgaacggttt ggccgtggcg tttgcccggc tcgtggtatt cagaatttcg cgcgcccagg
     4801 ccggtaactc gggattgatg cgatagtgga tccacagccc ctcgcgccgg tcgctgacga
     4861 tcccggcctc gcgcagcgtc gccagatggc gcgagatctt gggctgcgac tcatccaacg
     4921 cgtgagtgag ttcgcagacg cacaattcgg cttcgcccgc gagaagcaac aggcagcgca
     4981 agcgtgtcgc atcggcaagg gcggggaaga attcatcagc ggtaatcatg tattaaatat
     5041 acgtctggac atatatatcg tcaagagaat atattgatga cctcggtttt taccctggta
     5101 gattaggagt tgatatgcgt tacccgatcc gcgtcctgtt tctctgcacc ggcaattccg
     5161 tccgctcgca gatggccgaa ggtctgttgc gttccgtcgg tggcggagac ttcgaggttc
     5221 acagcgctgg caccgacccg aaggaactcc atccgctggc cgtcgaggcg atgcgggaga
     5281 ccggcatcga catctccggc catgagaaca agtcgctcga gcgtttcctc ggacaacact
     5341 ttgactacat catcacggtt tgcgatcgcg cgcgcgacag ttgcccgacc ttcccaggcg
     5401 acaaccagcg cattcactgg agattcgagg atccggcggc cgtgaccgga agccgggccg
     5461 agcagctcgc ggtatttcgg cgcgtgcgca acgagatttc cgagcgcctg cgcgtgtggg
     5521 tgacggtgca gcgcaagcta ctgaaggaga tcggccaccc ggcctgaggc ggcgtagccg
     5581 tcaggcaggt tcgccgccac gctcgggcga gaagtgcgcc gttacgatgc gcagcacgac
     5641 gagcgccatg atgacaccga ggccgagcaa caccttctcg ggcgcgtact cgcggaacca
     5701 cagtcccatg agctcgccga tcaggaccac gagtgcggtg tcgaggatga aggtgacgcg
     5761 tacgcgcccg agcttgaggt aggactgcaa ggtacggatg acctcgagca gtgccagcat
     5821 gatcagcgtg tcgatgatgg cgtgctcggc gacgctggcc cagccgcttg tcagtacctg
     5881 acgcaggtcg aggacgagca cgatcacgcc cgcgcccatc caggcgcata gcgcgacgat
     5941 ggcgagcccc gtgagcgaac gtacgagcac gggatagaag tggtcgacaa tctgaagcca
     6001 gagatcatta tcattagtta ccaatgcgcg tgcagttggc atgtcagatg tttccttgac
     6061 actattgccg tccggccctg atggagattt gacctggggc cgcatgattt tttgtgccag
     6121 gttgttcatg gatggaatat tagccgcggc ttatgaatgc cagatgaaat gtcgatgaat
     6181 tttttggttt gaaacggaga aatgaaatgc cggctcaaaa aagaaaggcc gccaaggcgg
     6241 cctttctgtc aggagctccg gggaggaggt cgccggttat gcatccatgc gcgcgcggct
     6301 cccggcgcgc tcgttgtatt ggcttcttac caccactgcc agccgtcctt cttgccgcgc
     6361 agtttgaagc cggtgatctt cagcacatcg tcagtgggat aaccgtcgac cgtcgacatg
     6421 ttggcatcgt cgctgtgctg gatcgataca tacgcggttc cgccatcggc gctgaatagg
     6481 aagccggtcg gttcagccga actgtctttg accgacataa ttttcacgca gccatccgtc
     6541 ttgatgtcgc gatccatgcc gtccggcagg caggcaaaga tgtcgccgtt gtcatggtct
     6601 tcaattacgt acacgttgcc agttttcggc tggaattcga ggttgtcgaa ggaattgaaa
     6661 tcagcatcgc cctcgatgaa gcggttcact accaccgtgc gctgtatatt gctggcggtg
     6721 agcgggacac ggtcaacgcc gcagacgacc tcggcgtagt tcgagccgtt ctcgttaccg
     6781 gtgttggtcc agcagaaacg caccgcttcc gggttaagcc cgtcctggta catcggatcc
     6841 tggtgcaggt cctccggccg gtagtaacgg gtggcaccgg cggcgttggc atcactacgc
     6901 gcggtggtcg cattgaccgc cacccaggcg gcattgccga tttcacagcc ttgaccgtat
     6961 tgctgggtgt tgttcacgca ggatacttgc atggcgtaca ccgatccggc cccgagcggc
     7021 gattcggaga ggttggtaat gggtcccgag acgaggcgtg gcatggctgg cacgaacttg
     7081 aagatcgcgc cgccgtcgcg gtcggcgccg cccgtgccgg gacgcaactc atcgccggcg
     7141 attacgacac cgttcgacaa caccgcgatc ccttcccagg ctatggttgg cagcgccgtg
     7201 cgtttggcga tgttggccgg gtcggtaacc atgccgctgg cgcggtcgat cacggtctgt
     7261 tcggtcacgc ccagcggatt gaatatttca tagacgccgc cgttcgaggt ttcctcggtg
     7321 gcgagggtag taccccaagc cgtggtgcgg atgccgtcgc agctggtcat cccgcgcaga
     7381 atggtttcga ccgcgccggt attgagatta atgcgctgta ccgacgggtt gagcttgtcg
     7441 ccggcgtcgt acacgttcgg tgtcccgccg tcgatcagga actggcgtcc accctcgacg
     7501 caggtgatga gatgagtcgg attctgcaac ggatagaacg ccatcatgtc ggtggcgttg
     7561 gcggcatcgc gcgtcaggta ttccaccttc aggccatgag cgaccagtgc ctggtcgtag
     7621 gctgtctgaa gcggtgtgcg gtacggtccg accgtgggcg aggccgaggc ttcgagcggt
     7681 ttttcgactc cgaacagttt ctcggactgc gccttgagga gttgctcgat cttgagaccg
     7741 aagtcagtgt catcgtcggc ctgagccgca aacgtggtga agatcgtggt catggccacg
     7801 gcgaatacgc aaggttttaa ttgaggtaca tgtttcatga tgtgactcct tgacgggttg
     7861 aatgattccg cgcaggagtc tagcgacgcg tcatgacgtg tgcgtgataa ttacgcgaca
     7921 aatgggtgac accacctcag gcggcgccgg aggcggagct atcagagtgt cgtgtgcggg
     7981 tggagggaaa gtaacaggtg aagctgctgc cgacgccggg ttcgctctcg atctcaagac
     8041 gtgcgtcgtg acgcagcagg acatgtttga cgatggacag accgaggccg gtgccgcccg
     8101 aggcgcgcga gcgcgccgtg tccacgcgat agaaacgctc cgtcagatgc gggacgtgtt
     8161 ccggggcaat gccaacgccg ctgtcgctga cggtgaagcg ggcgccgccg tccggggcca
     8221 tgccccagcg caatgcaata cggccgccag ccggcgtgta gcgcacggca ttgttgatca
     8281 ggttggaaaa ggcgctgagc agttcatcgc ggcttccgag cagccgcagg ttccggtccg
     8341 cctcgatcgt gatgacatga tgctgttcgc cgctcagcag ctccgcctgt tccttgagcc
     8401 cggccagcag cgatatgacg tccactttct cgtcgtgggt gcgcggcggc gcggtttcca
     8461 gtttcgacaa catgagcaga tcgtccacca ggcgctgcat gcgcaacgtc tgctcctgca
     8521 tggttgcgag gtgttttttc agctcctcca tgcgtatctg gtccatgtgg cccaaggtct
     8581 cgacgaaacc gccgagcacg gtgagcggcg tgcgcaactc gtgcgagacg ttggcgacga
     8641 aatggcggcg catctgttcg aggcgcgcga ggcgcgtgac gtcgcgcccg atcatgagct
     8701 tttgcaggct gccgaacggg gtgacctgga tggaaacgtg gatttcgctg ttttccgggg
     8761 aattgatttc cagcagctcg gaatagtcgc cgcgttgcag atattgaacg aaatcgggat
     8821 cgcgcaacag attgaacagg cgtgtaccga catcgcgcgg ataacggatg ccgaacaggc
     8881 gctcggcgga agggttggcc cactcgatgt cgccatcctg ggacaggatg accatcgcat
     8941 ccggcatggc cgcggcagcg ctctggaacc gggtcagcat gtcgttcagc tgatcttctc
     9001 tatgttccgt ttgcttcatg agctggcgga tttcattgaa cacgtcaccc cacaggcctt
     9061 cagcgtcggg gatttccgcc ttctggcggt tcaacagcca ataatgcagc cgctgcaggt
     9121 ggcgcatggt aaaaaagatg taaaggccga agccgatggc ggcgatgaga aaaggacgcc
     9181 cggtgatgag accgagcaca aggctggcaa tggcgatgcc cgccaggatc cagatttcac
     9241 gccagatggt ttgcggcacg ggtcaaccct gcgccgaaaa acgatagccg gtaccgcgca
     9301 cggtctggat cagccggtcg tggccggtgg gtgtcagtat tttgcgcagg cggcgtatgt
     9361 gcacgtccac agtgcgttcc tctacgtaca cgttgttccc ccagacgtcg ttcaacaggc
     9421 gctcgcgact gtgcacgcgc tcgggatggg tcatgaagaa gtgcaacagg cgaaattcgg
     9481 tcgggcccag gctgagggtt ttgtccgccg ccgtgacccg atgcgtcacc gggtcaagcg
     9541 acaacccgcc ggtttccacg gccacctccg ccgcgtgcgg cgaggtgcgg cgcaacaacg
     9601 cccggatgcg cgccagcagc tcgcgcgtgg aaaagggttt ggtgatgtaa tcgtccacgc
     9661 cactatcgag ccccctgacc ttgtcctcct cctcggtgcg cgcggtcagc atgatgacgg
     9721 ggatatcctc ggtcagcttc tcgcgccgca gctgccgggc gtaatccatg ccgctgacct
     9781 tcggcagcat ccagtcgagc aggatcaggt cgggtttgtc ggccaggatt tttgtctggg
     9841 cctcggtggt gtcggtcgcc tcgaggcagt cataatttgc ctgcgataaa gccagacaaa
     9901 ccatctgccg gatggcgggt tcgtcgtcga ctatcaggat tttgccgcgc atgatgtaaa
     9961 tagtcgggtc ttatgcccct ctgggtggcg ccatttcata ccataaatat tgcaatttgg
    10021 ttacccttac cattctatga cctttcgatg accgtggctg aaattccatt cagagtcagt
    10081 atagtagtga cgactttcat cccccgagca ggaaaagcat gagccagatt accgtcagac
    10141 cgaccgatat caaattgcac cagaaatcgc gcatccttga ggtggcgttc gatgacggca
    10201 gccatttcag gctgccttgc gaatatttgc gggtatattc gccctcggcc gaggtccgtg
    10261 gccattcgcc gggacaggaa gtgctccagg tcggcaagga gaacgtgaac atcacccaca
    10321 tcgagccggt cggtacttac gccatctgcc tgtatttcga cgatggtcac agcaccggga
    10381 tctattcctg ggactggctg catcagttgg gcaagaacca ggacagcctg tggaagcaat
    10441 acctggagcg gctcgccaag gccggccatc agcgcaagcc tgccaaagcg gctgagtaat
    10501 accatatgtc gcaaccaggc cataacgaac gcaagacgca cttcggtttt cgcgaggtgc
    10561 cggagtccca aaaggatggc cttgttgccg acgtgtttca ttcggtcgcc ggcaaatacg
    10621 acctgatgaa cgatctcatg tcgctcggcg cccatcgcct gtggaaacag ttcgcgacgg
    10681 aggcgagcgg ggtgcgcggt ggtttccgcg tcctcgatgt tgccggtggc accggcgaca
    10741 tggcggcgaa gtttgccccg cgtgttggaa ggcgtggaca ggtagtgatc gccgacatca
    10801 acagctccat gctggaagtt ggacgcggac gtttgacgga tcagggcttt gtgggcaatg
    10861 tcgattttgt gcaggccaat gccgagaacc tcccgtttcc cgagggctat ttcgaccgcg
    10921 tgagcatcgc cttcggcttg cgcaatgtga cgcacatcga ccgcgccctg tcctccatgt
    10981 tccgggtgtt aaagccgggc ggggcgctgc tggtgctcga attttcccgt cccgccgtgc
    11041 caaggctgaa ccgcctctac gacgcctatt cctttaatgt gctgccatgg ctcggacgcg
    11101 tggtggcgca ggacgaaggc agttatcgtt atctcgcgga gtcaatccgc aagcacccgg
    11161 atcaggaaac gctgaagggg atgatggagc gggctggctt cgagcgcgtg caatatttca
    11221 atttgagtgg cggcatcgtc gccctgcata aaggatataa gttttaatat ctctcaccgc
    11281 aaagacgcgg ggaatgcaaa gaaaagcgcg aagattttcc agtaataacc tttgcgcctt
    11341 tgcggtgaaa atttcttatt atgtcttcat cattgacaat gctgctcgaa gaggcgatca
    11401 atcgcgcgct gcggctcgat cccgccacgc tcgcgcgact cggcgagctc gaaggcaagg
    11461 tgattcgctt gcgggccgct ggcgaccgcc cttttgaaat tttcattctg ccggacgagt
    11521 ccggactgtg gttgcgcggc gaatatgacc gtgaaccgga cgtgacgctc actggagata
    11581 ttcccgtgat ctttcgtttt gcgctgcgcc ggatcattcc ggatgtcgtg gctgcgggtg
    11641 aagtgcagat cagcggtgac attgatctcg gccagcgttt ccagcggctg ctggagcagg
    11701 ccgatatcga ttgggaagag caggccgcgc gcgtgttggg agatgtacct gcacatcagc
    11761 tgggcaacgc cctacgcgat ctgggcggct gggccagacg gacactgcac acgctgaaac
    11821 aggatttgag tgaatacctt caggaagaaa gtcgcctgct gcccgtacgc ccgcgcgcgg
    11881 gtgcgttccg ccactccgtg gaaaccctgg agcacgacgt ggagagtctg gaaaaacgcc
    11941 tgacacgttt gcgcgagatg gcgcgatgaa acgtctgcgc cagttgggcc gcctgcttcg
    12001 catcacgcag attttcgtgc ggcacgatct cgacgagttc gtcaccgcca tccatctgtt
    12061 tcgtccctat cgcctgttgc tgcggctggc gccgtggcgc ctgttcgcgc gacgcaacat
    12121 tccgcgcggc gtgcgtctgc gcgaggcgct ggaggaactg ggaccgatct tcgtcaaatt
    12181 cggtcaaatg ctgtccaccc gtcccgatct gctgccggaa gacatcgcga gtgaactggc
    12241 acgactgcag gatcgcgtgc cgccgttttc gggcgacgaa tcggtgcggc tgatcgagca
    12301 ggccctcggg ggcaggctcg aaacctggtt cagcgagttc agccgcgaac ccatcgcctc
    12361 ggcctcggtg gcgcaggtgc acatcgcgcg cctgcacgac ggcgccgagg tggcggtcaa
    12421 ggtgctgcgt cctggcatcg aatcggtcat cgagcgcgat ctggagttgc tgtatttgct
    12481 ggcgcgacta gctatccgtt acgcgccgga ggcgcgccgt tttcgtccgg tcgaggtggt
    12541 ggatgaattc agcaagacca tccatgatga gctcgacctg cgggtggaat cggccaacgc
    12601 cagccgcctg cgtgccaact tcgaggactc gaagctgttg cacgttccca gggtttactg
    12661 ggacctgacg cgccgcacgg tgatggtgct ggagcggatt cacggcattc ccatcagcaa
    12721 cattgaaggt ctcaaggctg ccggcatcga catgcgccag ctggcgcaca acggcgtgga
    12781 gattttcttc acgcaggcgt tccgcgacgg tttttttcac gccgacatgc atccgggcaa
    12841 tatcttcgtt tcgcccgagg gccagtaccg ggccgtggat ttcggcatca tgggcacgct
    12901 cggcgagaag gacaaacgtt atctggcgga gaatttcctg gcgttcttca accgtgacta
    12961 ccatgccgtg gccgattccc atctgcgcgc cggctgggta gctccgggca ccaatcttgt
    13021 ggagttcgag gccgcgatcc gcaccgtctg cgagccggtg tttgcccggc ccatcaagga
    13081 aatttccttc ggccggttcc tgttgcacct gttccagacc gcgcgccgtt tcaacatgga
    13141 agtccagcca caactggtgt tgttgcaaaa gacgcttttc aatatcgaag gtctgggccg
    13201 ccggctttat cccgatctcg atctgtggga gaccgccaag ccttatctcg agcgctggat
    13261 gagtgagcac atgggcccgc gcgcctttct gaagagcctg cgcagggaat ttccaaaatg
    13321 gtgggcaatg ttgccggaaa tcccgtcgct ggtgcatgaa actctgcggc gcgcgggcga
    13381 agccgatgcg gcgcaggaat cgcgggcaca ggagcttgag cagcggctgc gccagatgcg
    13441 ttacagccag cgccggcttt atttcgccat tgccagcagc ggcttgctga tcgccggtgc
    13501 tgtattgctg ggaatcgata ccgtagcctc cgaggcgtgg gaatggcagc gggtgacggg
    13561 ctgggttctc gcagcttcag gggtttttat gctgctgcgc ggctggccgg gcagaaatcc
    13621 atgatcggcg gtatgtcggc cgttgtcgtg tttatgctaa actttgacca aattgcaaca
    13681 aatccatggt ccaatgaagg cgctcaacaa agctgttctc gcgagcgcgg ccctgctggt
    13741 ggccgccggt ccggctttgt ctgcgccgac cggcaagctt tcctggttta acgacacccc
    13801 tttcgcgggt gttggcgcct tgggtgtccc cgaagcgcgt ctgctcgacc agtcggacta
    13861 cacctctttc ggcatcaacc tttataactc ccggtataac tcccggcaga acgccgtgga
    13921 ggaaggctat ccggtggccc gtgaaatcac tctggatgcc aataccgtcc gcctttacgg
    13981 cgaggtcgaa tcgtcccgtt ccggggccgg cagtctgtct ccggtcgagg atttcccgct
    14041 cgggcgcgtc aacgcatttg gcgtgagctg gcaacatcgt ctgaatgcca tgaacagttt
    14101 cgcggtgtcg gcggaatacg gcgagggcac ggctgtttat ccgctgccgg tggatacgct
    14161 cgatacccgc gcggcgtttt cctggaccag cgttttgccg agcgccgtca agccgagcat
    14221 cacgggcagc gtttttctcg gcgatgaaat ggcgcgcgag gaaatctacc ggcatctcgg
    14281 acgccgttat tacggtttca ccgtcggcgg cagcctgacg ctgttccagt cgcacacgcc
    14341 ctatgtgtcc ttcaagatgc agcggagcct gtacgatacg gccgaagaaa ccttgctgat
    14401 ttctccgcgc agcggtgatc gttcgttgct gtcggcgggc tggaagtggc aggtgcagcg
    14461 cgatctcagt ctgcaggccg aggcgagtta cgggctgggc gaggtaccca gcagcagtct
    14521 ggatccctac agcctggagc gcagccgcat cctgttcggc acgcgcttcg gtttcaaata
    14581 aaaaaattca caggattcac aggattaaca agattaaaga tttaatagaa ttgatcctgt
    14641 aaatcctgcg aaatcctgtt aatcctgtct attcatttta tttttctctt tgtagtgcgg
    14701 cctcgacttg gccgcgcttt tccttcccgg ccagcgatcc aatcagctcc agc
//
LOCUS       DBNGZE010000083         4185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_913851, whole genome shotgun sequence.
ACCESSION   DBNGZE010000083 DBNGZE010000000
VERSION     DBNGZE010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_913851"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="AC16FV_02115"
     CDS             complement(<1..1011)
                     /locus_tag="AC16FV_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR2848889.1"
                     /translation="MPKSDPAAVAAASALPPEIFKAYDIRGIVGKTLTPAIVELVGQA
                     LGSEALAQNQKRFVIGRDGRLSGPELSAALARGIARSGCDVVDIGMVPTPVVYFAIQH
                     LGAGSGVAVTGSHNPPDYNGLKMVIGGVTLSGDLIQGLRARIESENLTTGQGKISQAD
                     VRTAYLERVAGDVKLARSMRVAMDCGNGVAGEMAPQLLKRLGCQVTELFCQIDGTFPN
                     HHPDPSKPENLEDLVAEIKKGGYDVGLAFDGDGDRLGVIAPDGHVIWADRQMILYARD
                     VLSRQPGGEIIYDVKCSRTLDAEIRKAGGKPTMWKTGHSFIKAKLRESGALLAGEMSG
                     HIF"
     gene            complement(1111..2631)
                     /locus_tag="AC16FV_02120"
     CDS             complement(1111..2631)
                     /locus_tag="AC16FV_02120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR2848890.1"
                     /translation="MAQSLTRVHFQVFLILLLCFAAIGYVGFLALRDTQQSSYREWAS
                     QVAGETTNRISALLTQQQQVLEKIAHQPAVSAVLQRGSSLERSRKEDEIKSQLSGAVS
                     VHLLSSHNAGVLSPTGPLDPVCLEYVRRMAGANTPASPEFHAVGTPAEHYDMVVPVRD
                     ENRNPSGYLLAGFARAPLQAILEQSLPRNAYMELQQPATGEDMQAVLTAGEISTARVP
                     TVASTLGIAHWTLIYQPAKAPPPILAGNRIYYLAAILVVLGVITVVLFRLYRRTITAV
                     RHDIHSLIRMFRDLREGSVRVDYPMALREFSEIFDYLRDRGQKLMQEKEKLKDMGLMD
                     HLSQLGNRRHFEARLKELFDASKANGPSSVLIIDMDYFKQVNDKHGHDAGDALIVGFA
                     DALRKVVRQTDVLARLGGDEFCIIYAYASLDKAKALAERLRRQLPREIHLPKGVIHQL
                     RWTGGLSAMHDKDAKPDDVLWRADQALFQAKEAGRNVTKVYDPVAGLAAKKQIMSS"
     gene            complement(2837..3295)
                     /gene="dut"
                     /locus_tag="AC16FV_02125"
     CDS             complement(2837..3295)
                     /gene="dut"
                     /locus_tag="AC16FV_02125"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR2848891.1"
                     /translation="MMHKVEVKILDKRIGAEFPLPKYATHGSAGMDLYACLDEHLHIS
                     PGETHLIPSGIAIHIGEADLAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFISVW
                     NRGREPFTIEPGDRIAQMVFVPVVQAQFDVVEEFEESRRGAGGFGHSGRK"
     gene            complement(3292..3951)
                     /gene="coaBC"
                     /locus_tag="AC16FV_02130"
     CDS             complement(3292..3951)
                     /gene="coaBC"
                     /locus_tag="AC16FV_02130"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF016051.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR2848892.1"
                     /translation="MVVTAGPTWDAIDPVRGLTNRSSGKMGYAIAEAAVEAGARVILV
                     SGPTSLSDPERIQTIRVESAQEMYDAVHAHIADADIFIGVAAVADYRPAQAAAGKIKK
                     MQETMTLELVRNPDILASVTALKPAPFTVGFAAETENLEQHARQKLETKKLDLVAANR
                     VGADLGFGADANSLLLIEHGSVTELPVQPKAKLARALIHHIAARYRAKSAAKVHTLRR
                     P"
     gene            complement(3958..>4185)
                     /locus_tag="AC16FV_02135"
     CDS             complement(3958..>4185)
                     /locus_tag="AC16FV_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR2848893.1"
                     /translation="LATESPVAVAPAMNQQMWSNTATQTNLATLKKNGVRIFGPAEGS
                     QACGEVGPGRMLEPADLVVMISELFASGELD"
BASE COUNT          752 a         1252 c         1336 g          845 t
ORIGIN      
        1 aaagatgtgc ccgctcattt cgcccgccag cagcgcgccg gattccctga gcttggcctt
       61 gatgaacgag tgcccggtct tccacatggt cggcttgcca ccggccttgc ggatttcggc
      121 gtcgagcgtg cgtgaacatt tgacgtcata aatgatttcg ccgccgggtt gacgcgacag
      181 cacgtcgcgc gcgtacagga tcatctggcg atcggcccag atcacgtggc cgtcgggtgc
      241 aatcacgccg aggcggtcgc catcgccatc gaaggccagg cccacgtcgt aaccgccctt
      301 cttgatttcg gcgaccaggt cttcgagatt ttccggcttg ctcggatcgg ggtgatgatt
      361 gggaaaggtg ccgtcgatct gacagaacag ttcggtgacc tgacagccga ggcgcttgag
      421 caattgcggc gccatttcgc ccgccacgcc attgccgcaa tccatggcca cgcgcatgga
      481 acgcgccagt ttgacgtcgc cagccacgcg ctcgagatag gccgtgcgca cgtcggcttg
      541 tgagatcttt ccctgtccgg tagtgaggtt ctcgctctca atccgcgcgc gcaggccctg
      601 gatcaagtcg cccgaaagcg tgacgccgcc gatgaccatc ttgaggccgt tgtagtcggg
      661 cgggttgtgg ctgccggtga ccgccacgcc cgagccggcg ccgagatgct ggatcgcgaa
      721 atacaccacc ggtgttggca ccatgccgat gtccaccacg tcgcagccgc tgcgggcgat
      781 gccgcgcgcc agcgccgcgg acagttccgg gccggacagg cgcccgtcgc ggccgataac
      841 aaaacgtttc tggttttgcg ccaatgcctc gctgcccagt gcttggccga caagctccac
      901 gatcgccggc gtcagcgttt tgccgacgat gccgcggatg tcgtaggcct tgaagatttc
      961 cgggggaagg gcagaggccg ccgcgacggc agcgggatcg gattttggca tggtttggtt
     1021 ttctggattg gatgcttctg ccgggcagtt taccgcatcc atatgtttca ggcgaggcga
     1081 acgaccccac gttgaaacac aacgccgatg tcagctggac atgatctgtt ttttcgcggc
     1141 caggccggcg acggggtcgt agaccttggt gacgttgcga ccggcctcct tggcctggaa
     1201 cagcgcctgg tcggcgcgcc acaggacatc gtccggtttg gcgtccttgt catgcatggc
     1261 gctcaggccg ccggtccagc gcaactgatg gatcacaccc ttgggcaggt ggatttcacg
     1321 cggcaattgc cggcgcaggc gctcggccag ggccttggcc ttgtccagcg aggcataggc
     1381 atagatgatg cagaattcat caccgccgag gcgcgcgagc acgtcggttt ggcgcaccac
     1441 cttgcgcagc gcatcggcga agccgacgat cagggcgtcg cccgcgtcat ggccgtgctt
     1501 gtcgttcacc tgcttgaagt aatccatgtc gatgatcagc acggacgagg ggccattggc
     1561 tttggaagcg tcgaacagct ccttgagccg cgcctcgaag tgacgccggt tgccgagttg
     1621 cgagaggtga tccatcagcc ccatgtcctt gagcttttcc ttttcctgca tgagtttctg
     1681 tccgcggtcg cgcaggtagt cgaaaatttc ggaaaattcc cgcaatgcca tgggatagtc
     1741 aacgcgcacg ctgccctcac gcagatcgcg gaacatgcgg atcagggaat ggatgtcgtg
     1801 acgtaccgcc gtgatcgtgc ggcggtagag gcggaacagc acaacggtga taacaccaag
     1861 aacgacaagg attgcagcaa gataataaat cctgttgccg gccaggatcg gcggcggcgc
     1921 cttcgccggc tggtatatca gggtccagtg agcgatgcca agtgtgctgg ccacggtggg
     1981 gacacgggcg gtggagattt caccggccgt gagcacggct tgcatgtctt ctcccgtcgc
     2041 cggctgttgc agttccatgt aggcgttccg cggcaacgat tgttccagga tggcctgcaa
     2101 cggcgcgcgc gcaaatcccg ccagcaagta tccgctcgga ttccggttct catccctgac
     2161 cggaaccacc atgtcgtagt gctcggcggg tgtaccgacg gcatggaatt ccggagacgc
     2221 gggtgtgttc gcgccggcca tgcgccggac gtactcgagg caaaccggat cgagcggtcc
     2281 ggtgggagac agaacaccgg cattgtggct tgacaacaga tgcactgaga cggcgccgct
     2341 cagctgggat ttgatttcgt cttccttgcg cgaccgctcc agggaggagc cgcgctgcag
     2401 caccgccgag acggcgggtt ggtgcgcgat tttttccagc acctgttgct gttgcgtgag
     2461 cagcgcgctg atccggttgg tggtttcacc cgccacctgc gacgcccatt cgcggtagga
     2521 cgattgctgc gtgtcgcgca gagcgaggaa gccgacgtag ccgatggccg cgaagcatag
     2581 cagcagaatc aggaagacct gaaagtgaac gcgtgtcagc gactgggcca tgggcgttaa
     2641 gagattccca ttgccggcct atgacaaagc gataaaggaa aacattcctt gttcatactc
     2701 taaagatagg ggacgccggt gtcggcagcc agtcgggcgc tccggcttcc gacccgtggc
     2761 gataaaccgc ggatacgcgt ggtaaatatt atgtcaagac gagcgagcgc agcgagcagg
     2821 gctacgttaa acgggactac ttccggccgg agtggccgaa accgcccgcg ccacggcggc
     2881 tttcctcgaa ttcctcgacg acgtcgaatt gcgcctgcac caccggcacg aacaccatct
     2941 gggcgatgcg gtcgccgggc tcgatggtga acggttcgcg cccgcggttc cagaccgaga
     3001 tgaatatctg gccctggtaa tccgagtcga tgaggcccac gaggttgccg agcacgatgc
     3061 cgtgtttgtg cccgagaccc gaacgcggca gcaataccgc ggcgagatcg gcctcgccga
     3121 tatgaatggc gatgccagaa ggaatgaggt gtgtttcacc ggggctgata tgcaggtgct
     3181 catctagaca tgcgtacaaa tccatacccg cggagccgtg cgtggcgtat ttcggcagcg
     3241 ggaattccgc gccaatgcgt ttatcgagga ttttcacttc cactttgtgc atcagggtct
     3301 ccgtagcgtg tggaccttgg ccgcgctctt cgcgcggtat cttgccgcga tatggtgaat
     3361 cagcgcgcgt gccagtttcg ccttcggctg gactggcaac tcggtgacgg atccatgctc
     3421 gatcagcagc aggctgtttg cgtccgcgcc gaagccgaga tccgcgccca cccgattggc
     3481 ggcgaccaga tcgagctttt tggtttccag tttctggcgc gcatgctgtt cgaggttttc
     3541 ggtttcggct gcgaagccga cagtaaacgg cgccggtttg agcgctgtca cgctcgccag
     3601 gatatcgggg ttgcgcacca gctccagcgt cattgtttcc tgcatcttct ttatttttcc
     3661 ggccgctgcc tgtgccgggc ggtaatcggc gaccgccgcc acgccgatga aaatgtcagc
     3721 gtccgcgata tgggcatgca cggcatcgta catttcctgc gcgctctcga cgcggatggt
     3781 ttggatgcgt tcgggatcag atagtgacgt tggaccggaa accaaaataa cccgtgcccc
     3841 ggcttcgacg gcggcttcgg cgatggcata acccatcttg ccggaactgc ggttggtcag
     3901 cccgcgcacc gggtcgatcg cgtcccaggt gggaccggcc gtgacaacca cggtaagtca
     3961 atcgagttcg ccgctggcga acaactcgga aatcattact accagatcgg ccggttccag
     4021 catgcggccg ggtccgactt caccgcaggc ctgcgatcct tccgccggcc cgaaaatcct
     4081 gacgccgttt ttcttgagtg tcgccaggtt ggtctgggtc gcggtgttgc tccacatctg
     4141 ctgattcatg gcaggcgcga cggccacggg cgattcggtc gcgag
//
LOCUS       DBNGZE010000084         5423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1260921, whole genome shotgun sequence.
ACCESSION   DBNGZE010000084 DBNGZE010000000
VERSION     DBNGZE010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1260921"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..520
                     /locus_tag="AC16FV_02140"
     CDS             173..520
                     /locus_tag="AC16FV_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR2848894.1"
                     /translation="MPVAARPGVLSLTIKDKNALYAAYMPFVKGGGIFVPSNRPYKLG
                     EEVFMLLTLMDSKEKIPVAGHVVWVTPPGAQGSRTAGIGIQFSEKDSGIARNKIEGIL
                     AGALNSDRLTHTM"
     gene            528..1307
                     /locus_tag="AC16FV_02145"
     CDS             528..1307
                     /locus_tag="AC16FV_02145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR2848895.1"
                     /translation="MLVDSHCHLNFEPLNAGLDDVLQRARDNGVGHMLCVSVTLETFP
                     EIRALAHDHSNLSASVGVHPNEREGREPSVEDLIGLANDARVVAIGETGLDYYRSQGD
                     MTWQQERFRRHIRAAKKSGKPLIIHTRDAATDTLRIMREEGAGDIGGVMHCFTESWEV
                     ARAALDLNFYISFSGIVTFRNAEALREVAKQVPQDRLLIETDAPYLAPVPFRGKTNEP
                     AFVKYVAEYLAMLRATNFEDIAGYTTRNFFTLFKQANSPAQ"
     gene            complement(1333..1797)
                     /locus_tag="AC16FV_02150"
     CDS             complement(1333..1797)
                     /locus_tag="AC16FV_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2848896.1"
                     /translation="MYKRILVPVDGSTTSMNGLEKAMRLAKTSKARLRILHVVDGIAF
                     SRDHSMFTATAEKFRESGRKLIRDVMARVKKQQVQADSRMVENLTGRAADTIVKQANK
                     WKADVIVMGTHGRRGFNRLMLGSDAELVARTASAPVLLVQPSRKIQRGGKKK"
     gene            1988..3124
                     /gene="tal"
                     /locus_tag="AC16FV_02155"
     CDS             1988..3124
                     /gene="tal"
                     /locus_tag="AC16FV_02155"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR2848897.1"
                     /translation="MHNPLLVIKSLGQSIWLDYIERGMFASGELAHLIRRDGLAGITS
                     NPAIFEKAITGHEDYEADIAALARHARNATEIYEPVVLDDIGHAADLFLPVYEQTQGR
                     DGFVSLEVSPHLAYDTDKTVKEAIRLWERLHRPNIMIKVPGTVQCLPAARQLIAKGIN
                     VNVTLLFGLGRYREVLQTWLAGLEDRVSAGLSLESVASVASFFLSRIDVLVDKHLDAL
                     AQSGDKSASSLRGTAAIAYARLAYQIYREMQASVRWCGLAARSAQPQRLLWASTGTKD
                     PAYSDVKYVEPLIGSDTVNTLPPETLNAYRKYGDPALRLDQDLAQAAEVPSKLAKLGI
                     DLEAVAQKLEEEGVKKFIEPFDKLLASLEQRRQKLTPVSKDISL"
     gene            3317..3655
                     /locus_tag="AC16FV_02160"
     CDS             3317..3655
                     /locus_tag="AC16FV_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848898.1"
                     /translation="MFEHRSDTLLPYPIFIRRVIQHVGFAGVILVAALGIGTLGYHSL
                     ENLSWVDALLNASMILGGMGPVDTLHTTVGKLFASAYALFSGIVFLATSGVLLAPFLH
                     RLIHRFHLEK"
     gene            complement(3678..5045)
                     /locus_tag="AC16FV_02165"
     CDS             complement(3678..5045)
                     /locus_tag="AC16FV_02165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HIR2848899.1"
                     /translation="MTDHYAVVWARTAAEPRKMGNLVATGREMRFSYAPEFLEQGDIA
                     GLSLLLPPSLWRERPFVHQSTEILPLLPRLMALIPGRNANNIQRRLYTSLLAKRSNPP
                     AQGFETEWELLMLTGHNGIGHIDVFPDDRAAQATYADKDMPKKRVGSRSEFWKFLKEG
                     TSQDFSDETLDIMRLIGPTPSVGGQIPKLLVAIPDKDSWDGSLAEPGTHAINDVPYVD
                     VVMKVEDPQYRGLAALEALCLDVHRELGFEVPRQWRATIDGLSVLAVERFDRAPSGHP
                     IAMESFFSVYATGQQQVRTMTDAEVEGVGLMLLKLAEIADIDPAAARLEVYRRFVMAL
                     LTGNGDLHLENLAFLGGGPEKIRVSPVFDPTPMRAWDRHNLISALPFYIDEKQSLGYS
                     VARVGESFGLTRAVAADILYELLKTTRDYPERVLALKDVPEVNRKNLANRVKSLRSRL
                     KGVHP"
     gene            complement(5042..5245)
                     /locus_tag="AC16FV_02170"
     CDS             complement(5042..5245)
                     /locus_tag="AC16FV_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2848900.1"
                     /translation="MAETRKLKEKELAKLAGLRAETLSRMKSRGTADFTLIDRLARVV
                     GLKLAVVPDDDQLARIQQGRFFT"
BASE COUNT         1150 a         1555 c         1521 g         1197 t
ORIGIN      
        1 cttgagaatt accggcagct caacagcccg ctcgacgaat ttttgatgct cgaggacatt
       61 ttgatacgct gggttcgatt gagccgacta caatgaaatt gaactgtagt ttcccatgaa
      121 cgacaaaacc accaccccac ataccgccgg cgctgccaag gcggccaaac ccatgccggt
      181 cgcggcgcgt cccggcgttc tgtccctgac catcaaggac aaaaacgccc tgtacgcggc
      241 ctacatgccg ttcgtgaagg gtggcggcat cttcgtcccg agcaaccgcc cgtacaagct
      301 tggcgaagag gtgttcatgc tgctgacgct gatggacagc aaggaaaaga ttccggtcgc
      361 cggccacgtg gtgtgggtca cgcctccagg cgcgcagggc agccgcaccg ccggcatcgg
      421 catccagttc agcgagaagg attcgggcat cgcgcgaaac aaaatcgagg gcatcctcgc
      481 cggcgcgctc aattccgacc ggctcacgca cacgatgtaa cgtgacgctg ctcgttgact
      541 cccactgtca tctcaatttt gaacctctaa acgccggcct ggatgatgtc ctccagcgcg
      601 cccgggataa cggggtcgga cacatgctgt gcgtgtcggt cacgctggaa acctttcctg
      661 agatacgtgc gctcgcgcac gaccactcaa acctgtctgc ttcggtgggc gtgcatccca
      721 atgaacgaga agggcgcgaa ccttccgtgg aagatctgat cggcctcgcc aatgacgcgc
      781 gcgtggttgc catcggcgag accggactcg actattaccg cagccagggt gatatgactt
      841 ggcagcagga gcgcttccgc cgccatatcc gcgcagcgaa gaaatcgggt aagcctttaa
      901 ttattcacac ccgcgatgcc gccactgaca ccttgcgcat catgcgcgag gaaggggcgg
      961 gcgatatcgg cggcgtgatg cactgcttca cggagagctg ggaggtggcg cgcgcggcgc
     1021 tcgatctcaa tttctatatt tctttttccg gtatcgtgac ctttcgtaac gcggaagcct
     1081 tgcgcgaggt ggcgaaacag gttccgcagg atcgtttgtt gatcgaaact gacgcacctt
     1141 atctggcgcc ggtgccattt cgcggaaaga ccaacgagcc ggcgttcgtg aaatatgtcg
     1201 cggaatacct ggccatgctg cgcgccacga actttgaaga tatcgccggg tacacgaccc
     1261 ggaatttctt cacgcttttc aaacaggcaa attctcccgc gcaatgaaat cccctggctg
     1321 aatcgtgccg ggttattttt tctttcctcc gcgctggatt tttctggaag gctgaaccag
     1381 cagaaccggg gccgaagcgg tgcgcgcgac gagttcggca tcgcttccga gcatcagccg
     1441 gttgaacccg cggcgtccat gcgtgcccat cacgatcacg tcggccttcc acttgttggc
     1501 ttgtttcacg atcgtgtccg cggcgcggcc cgtgagattt tccaccatgc gcgaatcggc
     1561 ctgcacctgt tgtttcttga cgcgcgccat gacatccctg atcagttttc tgcctgactc
     1621 gcgaaatttc tcggcggttg cggtgaacat gctgtgatcg cgggaaaagg cgatgccatc
     1681 caccacatgc agaatccgca gcctggcttt gctggtcttg gccaggcgca tggccttttc
     1741 caggccattc atggaggtcg tgctgccatc cacgggaacg agtatgcgtt tgtacatgtc
     1801 tccaccttcc tttttatgaa ttatcggctt ttggcgcggt agacattgtt gattctcgtc
     1861 aaatcaaggc cataacggaa tgccatcgga acagcgatat acgtcatttc ttttgaaata
     1921 tttatctcgc cacgagacac aggtagactt ggtcaccgtt tggtccttca gggaaaggag
     1981 gggcaggatg cacaatccct tgcttgtcat aaagtctctg ggccaaagca tctggctcga
     2041 ctatatcgag cgcggcatgt tcgccagcgg cgaactcgcc caccttatca ggcgcgacgg
     2101 cctcgcgggc atcacatcga acccggccat attcgaaaag gccatcaccg gccacgaaga
     2161 ttacgaggcg gatatcgccg cgctggcgcg tcatgctcgc aatgccaccg agatatatga
     2221 acctgttgtg ctggacgaca tcggccatgc cgccgatctg tttctgccgg tgtacgaaca
     2281 gactcagggg cgcgacggct tcgtcagtct cgaagtatca ccgcatctgg cctatgacac
     2341 ggacaagaca gtaaaggagg ccatccggct gtgggaaagg ctgcaccgcc cgaacatcat
     2401 gatcaaggtt ccgggcaccg ttcagtgcct gcccgccgcg cggcagctca tcgccaaggg
     2461 catcaacgtc aacgtgaccc tgctgttcgg tcttggccgt tatcgcgagg tgttgcagac
     2521 atggttggcc ggtctcgagg accgtgtcag cgccggtttg agcctggagt cggtggcctc
     2581 ggtcgcgagc ttttttctta gccgcatcga tgtgctggtg gacaagcacc tcgatgcact
     2641 ggctcagtct ggcgataaat cagcttcctc cttacgcggg acggcggcca tcgcctatgc
     2701 acgtctggcc tatcagatat accgggagat gcaggcctct gtgcgctggt gcgggctggc
     2761 tgcccgcagc gcccagcccc agcgcctgtt atgggccagc accgggacca aggacccggc
     2821 ctatagcgac gtgaaatatg tcgaacccct gatcggatcg gacacggtca acaccctgcc
     2881 gccggaaacg ctgaatgctt accgcaagta cggcgacccg gccctgcggc tggatcagga
     2941 tctggcgcag gctgccgagg tgccatccaa gttggccaaa ctggggatcg atctggaggc
     3001 tgttgcccaa aaactggagg aagagggagt aaaaaaattc attgaaccgt tcgacaagct
     3061 gcttgcttca cttgaacaac gccgtcagaa gctcacacca gtatcaaaag atatttcttt
     3121 ataaacaatt aatttcatat tttctttcat gttatggagg aaataaaaac taattcagga
     3181 acgagatgga atgttcccgg aggagcatta gcagagacag gaatatgtag ccattgggct
     3241 tgtttctcag cttggtaaat gctgatattc gtataatgta tattatgtaa aattatgaaa
     3301 agagctcaat aacatcatgt tcgagcaccg cagcgatacc ctgctcccgt accccatctt
     3361 cattcgccgt gtcattcagc acgtcggttt cgccggcgtg attctcgtcg cggcattggg
     3421 tatcgggacg ctcggctatc actcactgga gaatctgtca tgggtggatg cgctgctcaa
     3481 tgcctccatg atcctgggcg gcatgggccc ggtggacacg ctgcatacga ctgtcgggaa
     3541 actcttcgct tcggcttacg cactgttcag tgggattgta tttctggcca cgtccggggt
     3601 tctgctggcg ccgttcctgc atcgcctgat ccaccggttt catcttgaga agtaatttgc
     3661 caggttcctt gggcagatca tgggtgaacg ccttttaatc tcgaacgaag cgacttcacc
     3721 cggtttgcca gatttttccg gttaacttcc ggtacgtcct tgagcgccag cacccgttcg
     3781 gggtaatccc tggtcgtctt cagcagctca tacaggatat ccgccgccac ggcacgggta
     3841 aggccgaagg attcacccac gcgcgcgacg ctgtagccaa gggactgttt ctcgtcgatg
     3901 taaaacggca gcgccgagat gagattatgc cgatcccagg cgcgcatggg cgtgggatcg
     3961 aacaccggtg atacgcgaat cttttccgga ccacctccca ggaaagccag attttccaaa
     4021 tgcaggtcgc cattgccagt gagcagtgcc atcacaaagc gccggtagac ctcgagacgc
     4081 gccgcggcgg ggtcgatatc ggcgatttca gccagtttca gcaacatcag gcccacgcct
     4141 tcgacctcgg catcggtcat ggtgcgcacc tgctgctggc cagtggcata caccgagaaa
     4201 aaactttcca tggcgatagg atggccggat ggcgcgcgat cgaaacgttc cacggccaga
     4261 accgacaggc cgtcgatggt ggcgcgccat tggcgcggta cttcaaagcc cagctcgcgg
     4321 tgcacatcga gacacagggc ctccaatgcc gccagaccgc ggtactgcgg gtcttcgacc
     4381 ttcatcacca cgtccacata cggaacgtca ttgatggcgt gggtcccggg ttcggccagg
     4441 ctgccatccc aagagtcctt gtccggaatc gctaccagca gcttggggat ctgccccccc
     4501 accgagggcg tggggccgat cagccgcatg atatcgaggg tttcatcgct gaaatcctga
     4561 ctcgtgccct ccttcaggaa tttccagaat tccgagcgcg aaccgacccg tttcttgggc
     4621 atgtccttgt ccgcgtaggt tgcctgcgcc gcgcgatcgt ccgggaacac gtcgatatgt
     4681 ccgatgccat tgtgcccggt gagcatcaac aattcccatt cggtttcaaa tccttgtgcg
     4741 ggagggttcg agcgttttgc aagcaagctg gtatacagcc ggcgctgaat attgttggca
     4801 ttgcgaccgg gaatgagcgc catcagccgt ggcagcaaag gcaagatctc ggtggattga
     4861 tgcacgaagg gccgctcgcg ccatagcgat ggcggcaaca agagcgacaa gccagcgata
     4921 tcaccctgct ccaggaattc cggggcgtaa gaaaaacgca tctcgcggcc ggtcgccacg
     4981 agattgccca tcttgcgagg ctcagcggcg gtgcgggccc acaccactgc gtaatggtcg
     5041 gtcatgtgaa gaagcggccc tgctggatgc gcgcgagttg gtcgtcatcc ggcaccacgg
     5101 cgagcttcag tcccacgacc cgcgccaggc gatcgatgag cgtgaaatcg gcggtgccac
     5161 ggcttttcat gcggctcagg gtttcagcac gcagccccgc gagcttcgcc agttcctttt
     5221 ctttgagctt tctggtttcg gccaccgcca ggatggcgtt tagggttgac cgtaccgggt
     5281 cagaattgcc atgagattta atattgatca taaaatcaat ttaaatcata acttgattta
     5341 tatgtcaata tttatattta aatgactata tagtcaataa tataaattat ataaaaatat
     5401 agaaatttat atatttaatg tca
//
LOCUS       DBNGZE010000085         4025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_844560, whole genome shotgun sequence.
ACCESSION   DBNGZE010000085 DBNGZE010000000
VERSION     DBNGZE010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_844560"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..791)
                     /locus_tag="AC16FV_02175"
     CDS             complement(123..791)
                     /locus_tag="AC16FV_02175"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR2848901.1"
                     /translation="MSRESWKIFQIMAEFVAGFEHLSQIQPSVSIFGSARTPPEHPYY
                     ALTEEVARKLSDAGFSVVSGGGPGLMEAANKGAYAGKSPSVGLNIRLPHEQHANRYQN
                     ITLTFQHFFARTVMFVKYAKAYVVIPGGFGTLDEVIEALTLIQTGKSTRMPIILVHAP
                     FWSGLLDWFRGTLIREGVIDENDMSLLSVVNSADEVLDVIFRYYEECGFEASEAEQEI
                     KLNL"
     gene            984..3740
                     /gene="polA"
                     /locus_tag="AC16FV_02180"
     CDS             984..3740
                     /gene="polA"
                     /locus_tag="AC16FV_02180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR2848902.1"
                     /translation="MSPKATVRKKKAATEVPAGGKKDGPLVLVDGSSYLYRAFHAMPA
                     LANSRGEPTGAVYGVTNMLRRLLSEYDTEYLAVVFDARGKTFRDDIYPEYKAHRPPMP
                     DDLSVQIEPIHAIVRALGLPLIQIEGVEADDVIGTLARAATAEGRATVISTGDKDMAQ
                     LVDGHVRLVDTMKDAEYDRDAVVAKFGVTPERIVDYLALVGDTSDNIPGVPGVGPKTA
                     AKWLQEHGSLDAVMAHAADVPGKAGESLRASLGHLPMARQLATIKCDVDLKLAPNELK
                     RTPPDEARLRELFSRLEFKSWLAELGGANAPTAASAPTAMNAKYQMITDMKVFDQWLA
                     RLADSKPFAFDTETTSLDAMQAEIVGVSFTDRAGEGAYVPLAHEYPGAPAQLGRDAVL
                     AKLKPLLEDEAHSKIGQNFKYDMSVLANYGIALAGMRYDTMLESYVLDSTASRHDMDS
                     LALKYLGYKTVKYEEVAGKGKHQINFREVPLDQATAYAAEDADVTLRLHEHLWPRLNQ
                     TPTLVKLFDEIEMPLVPVLARMERHGVLIDVAMLRAQSNELGKRLIEIEAESHKLAGQ
                     PFNMGSPKQIQEVLYDKHKLPVLKKTPTGQPSTAEEVLQELALDYPLPQLILEFRSLA
                     KLKSTYTDKLPEMIDPKTGRVHTSYHQAVAATGRLSSTDPNLQNIPVRTAEGRRIRQA
                     FIAAPGYRIMSADYSQIELRIMAHLSGDKGLIHAFANNMDVHRATAAEVFGVAPDKVS
                     DDMRRAAKAINFGLIYGMSAFGLAKQLRIERVAAQEYVERYFARYPGVREFMERTRET
                     ARRQGYVETLFGRRLHTSEINAANMNRRQAAERAAINAPMQGTAADLIKRAMIAVDRW
                     IPKSVLDIRMVMQVHDELVFEVAEDAVDAAREHVRNAMSGVATLSVPLVVDIGVGGNW
                     DEAH"
BASE COUNT          785 a         1249 c         1261 g          730 t
ORIGIN      
        1 gggcacgctg gcggccggtg ccttgctttt ggcgccgttc gacgccggtt cggccgccat
       61 cgccggcgca atcagggcag caagaatgag caggaatgtg atgcgcatgt tcgtccgtgt
      121 attcaaaggt tcagcttgat ctcttgttcc gcttccgagg cctcgaagcc gcattcctca
      181 taataccgga agatcacgtc gagtacctcg tcggcgctgt tcaccacgct cagcaggctc
      241 atgtcgttct cgtcaatgac gccttcgcgg atgagcgtac cccggaacca gtcaagcagg
      301 ccggaccaga acggcgcgtg caccaggatg atgggcatgc gcgtgctctt gccggtctgg
      361 atcagcgtga gcgcctcgat cacttcgtcg agcgtgccga agccgccggg tatgaccacg
      421 taggctttgg cgtatttcac gaacatcacc gtgcgcgcga agaagtgctg gaacgtgagc
      481 gtgatgttct ggtagcgatt ggcatgctgc tcgtgcggca ggcggatgtt cagcccgacg
      541 ctcggcgact tgccggcgta cgcgcctttg ttggcggcct ccatcagccc cgggccgccg
      601 ccgctgacca cggagaatcc ggcgtcggaa agtttgcgcg ccacctcctc ggtcagggca
      661 taatacggat gctcgggcgg cgtgcgcgcg gaaccgaaaa tgctgaccga gggctggatc
      721 tgcgacaggt gctcgaagcc cgcaacaaac tcggccatga tctggaagat tttccaggat
      781 tcgcgcgaca tggtgcccga gggcgcgatc tcgacgcgga actgggagtc cactccgttg
      841 ggtttggggt acggcggttc tttatctagg ggatttccgt cattcatagc tgtagcccta
      901 cttcgctcgt cttatcataa aatacatttt tagcacaaat aattagaccc cgggtgaccc
      961 ggcgaattca tgtaccccga atcatgagcc ccaaagccac agtccggaag aaaaaagccg
     1021 ccacggaagt gccggcgggt gggaagaaag acgggccact cgttctcgtg gacggttcat
     1081 cctatctcta ccgcgccttc catgccatgc cggcgctcgc caattcgcgc ggcgagccca
     1141 ccggcgcggt ctacggcgtg accaacatgc tgcgccgcct gctttcggaa tatgacaccg
     1201 aatatctggc ggtggtgttc gacgccagag gcaagacctt ccgcgacgac atctaccccg
     1261 agtacaaggc gcaccgcccg ccgatgccgg acgatctctc ggtccagatc gaaccgatcc
     1321 acgccatcgt gcgtgccctc ggcctgccgc tgatccagat cgaaggcgtg gaggccgacg
     1381 acgtgatcgg cacgctggcg cgcgccgcca cagccgaagg gcgcgcgacc gtgatctcga
     1441 ccggcgacaa ggacatggca cagctcgtag acgggcatgt ccggctggtg gacaccatga
     1501 aggacgccga gtacgaccgc gacgcggtgg tggcgaagtt cggcgtgacg ccggaacgga
     1561 tcgtggatta cctcgcgctc gtgggcgaca cctcggacaa cattccgggt gtacccggcg
     1621 tcggtcccaa aaccgcggcc aaatggttgc aggaacatgg ctcgctcgac gccgtcatgg
     1681 cgcatgcggc tgacgtgccc ggcaaggccg gcgagagtct gcgcgcgtcg ctgggtcatc
     1741 tgccgatggc gcgccagttg gccactatca agtgtgatgt ggatttgaaa ctcgcgccca
     1801 acgaactcaa acgcacgcca ccggacgagg cgcgcctgcg tgagctgttc tcgcgtctcg
     1861 aattcaagtc gtggctggcg gaactgggcg gcgccaacgc cccgacggct gcgtccgcgc
     1921 cgacggccat gaatgcgaag taccagatga tcaccgacat gaaggtgttc gatcagtggc
     1981 tggcgcggct cgccgactcg aaaccgttcg ccttcgatac cgagaccacc agtctcgacg
     2041 cgatgcaggc cgagatcgtc ggcgtgtcgt tcaccgatcg tgccggggag ggcgcctacg
     2101 tgccgctggc gcacgaatat cccggcgcgc cggcgcagct cggccgtgat gcggtgcttg
     2161 caaagctgaa gccactgctg gaagacgaag cccattccaa gatcgggcag aacttcaaat
     2221 acgacatgag cgtgttggcc aattatggta ttgcgttggc cggcatgcgc tacgacacca
     2281 tgctcgaatc ctacgtgctg gactcgaccg cctcgcgcca cgacatggat tcgctcgcgc
     2341 tcaagtatct cggctacaag acagtcaaat acgaagaggt tgccggtaag ggcaagcacc
     2401 agatcaattt ccgcgaggtg ccgctggacc aggccacggc ctacgccgcc gaggacgccg
     2461 acgtcacctt gcgcctgcat gaacatctgt ggccgcgcct gaaccaaacg cccacgctcg
     2521 taaagctttt cgatgagatc gaaatgccgc tggtgccggt gctggcgcgc atggagcgcc
     2581 acggcgtgct catcgacgtc gccatgctgc gggcgcaaag caacgagctg ggcaagcgtc
     2641 tgatcgagat tgaagccgag tcgcacaagc ttgcgggcca gccgttcaac atgggctcgc
     2701 ccaagcagat ccaggaagtg ctgtacgaca agcacaaatt gccggtactc aagaaaacgc
     2761 ccaccggcca gccgtccacg gcggaagagg tgttgcagga actggcgctg gactatccgc
     2821 tgccgcagct cattctcgag ttccgcagcc tcgccaagct caagtccact tataccgaca
     2881 agctgccgga gatgatcgac ccgaaaaccg ggcgcgtgca tacctcgtat catcaggcgg
     2941 tggcggcgac cgggcggctg tcctcgaccg atcccaacct gcaaaacatc ccggtgcgca
     3001 cggccgaagg gcggcgcatc cgccaggcgt tcattgccgc gcccggctac cgcatcatgt
     3061 cggcggatta ttcgcagatc gagctgcgca tcatggcgca tctctcgggg gacaaaggac
     3121 tgattcatgc gttcgccaat aacatggacg tgcatcgcgc caccgccgcg gaggtgttcg
     3181 gcgtcgcgcc ggacaaggtg agcgatgaca tgcgccgcgc cgccaaggcg atcaattttg
     3241 gattgatcta cggcatgtcg gccttcgggt tggccaagca gttgcgcatc gagcgcgtgg
     3301 cggcgcagga atatgtcgag cgctatttcg cgcgctatcc cggcgtgcgc gagttcatgg
     3361 agcgcacgcg cgagaccgcg cgccggcagg gttatgtcga gacgctgttc ggccggcggc
     3421 tgcatacctc cgaaatcaac gccgccaaca tgaaccggcg ccaggccgcc gagcgcgccg
     3481 cgatcaacgc gccgatgcag ggtacggcgg cggatctcat caagcgagcg atgatcgcgg
     3541 tggatcgctg gattccaaaa tccgtcctcg acatccgcat ggtcatgcag gtgcatgatg
     3601 aactggtgtt cgaggtggcg gaggacgccg tggacgccgc gcgcgaacat gtgcgcaatg
     3661 ccatgagtgg cgtggcgaca ttatccgtac cgctggtggt ggatatcggc gtgggcggca
     3721 actgggacga ggcacattag cgcgccgcca ttcgcgattt gcgatcggag gcggacaaac
     3781 acacatgaaa cttacgtgaa gcggcaggcc caagttttac catccgcaac gaaaggcttg
     3841 aactattcca gtggtagtgc gtctataatg cgcagacgat ttggatatcg tcttgccccg
     3901 ctccacctcc cttagcgggg tacgttccgg atgcacatca tccgggacat gttcggcccc
     3961 ggacactctc cccttgtctc cggggctttt tttgtgagcc acatccctgt ggctcaccct
     4021 cgccg
//
LOCUS       DBNGZE010000086         2224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1400552, whole genome shotgun sequence.
ACCESSION   DBNGZE010000086 DBNGZE010000000
VERSION     DBNGZE010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1400552"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="AC16FV_02185"
     CDS             <1..574
                     /locus_tag="AC16FV_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848903.1"
                     /translation="AGQVLLFGQDVTTWPSHRRTALGMARTFQVTSLFPKLTVLDNVL
                     LAIKGLRPSKFVMWRFLSSYTEVYDKAHHLLEQANFLDRKDTEVRNLSHGEQRQLEIV
                     LGLASDPKILLLDEPAAGLSSGESTEMSEFLMKLDPTLALLLIEHDMDVVFDVADEIS
                     VLHFGEILETGSPESIKKSRRVQEIYLGSD"
     gene            579..1280
                     /locus_tag="AC16FV_02190"
     CDS             579..1280
                     /locus_tag="AC16FV_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848904.1"
                     /translation="MAILEVQDIHTYYGDAYVLQGLSLKLEQGQILGLLGRNGVGKTT
                     LVNSIVGFNPPRRGKVVFKGDDITYQASFETVRGGMGLVPQGRRVFPTLGVEENLLVA
                     ERSPDRHKWNLDRVYKLFPRLHERRYQRAKTLSGGEQQMLAIGRALMTNPDCLIMDEP
                     SEGLAPIIIQGLWEAIGTLKKEGLSILLVEQNAHLALKLVDYVHVMSKGQVVYSAKPE
                     ELRANDEIKSSYLGI"
     gene            1557..>2224
                     /locus_tag="AC16FV_02195"
     CDS             1557..>2224
                     /locus_tag="AC16FV_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2848905.1"
                     /translation="MAGTAAGAVAATFKFPAPAIAQAAPFKLGLLTVKTGPLAQGGIQ
                     MEQGIVTYLKEKNYTFAGRKVEFISADTGGNPAGTKTKAQELIERDKVDTIVGPLAAF
                     ELLAITDYIAQQKTPLLSLAAAEDMTQRRPNPYFLRASATSAQAMHPMAEFAAKELKL
                     KRMITISEDFAFGYEQMGGFQQTFEENGGKILNKLWPPIVTPDYTPYLAQIQDCDGVC
                     QGFAG"
BASE COUNT          494 a          652 c          645 g          433 t
ORIGIN      
        1 cgccggacag gtgctgctgt tcggccagga cgtgacgacg tggccgagtc accgtcgcac
       61 cgcgctcggc atggcacgca cgttccaggt cacgagtttg tttccgaaac tgaccgtgct
      121 cgacaacgta ctactggcga tcaagggcct gcggccgtcg aaattcgtga tgtggcgctt
      181 cttgtcctca tacacagaag tctatgacaa ggcgcatcac ctgctcgaac aagcgaattt
      241 cctcgaccgc aaggataccg aggttcgcaa cctgtcgcac ggcgagcagc gccagctcga
      301 gatcgtgttg ggtctcgcca gcgatccgaa aattctttta cttgatgaac cggctgccgg
      361 cctgtcgtca ggcgaatcta cggagatgtc agaattcctg atgaagctcg atcccacgct
      421 cgcactcctc ctaattgaac atgacatgga cgtggtattc gacgtcgctg acgagatctc
      481 ggttctgcat ttcggcgaaa tactggaaac tggatcaccc gagagcatta agaaaagccg
      541 tcgcgtacag gaaatttatc tggggtccga ctgacgccat ggctattctc gaagtccagg
      601 acatccatac ctattacggc gacgcctacg tcctgcaggg cttatcgcta aagctcgagc
      661 aaggtcagat cctcggtttg ctcggccgca acggcgtcgg aaaaaccacg ctggtgaact
      721 cgattgtcgg ctttaatccg ccgcgtcgcg gcaaagttgt attcaagggc gacgacatca
      781 cgtaccaggc gtcatttgaa accgtgcgag gcggcatggg tctggtgccg cagggacgcc
      841 gtgtttttcc gacacttggg gttgaagaga acctgctggt ggccgagcgc agtccggatc
      901 ggcacaagtg gaatctggac cgtgtctata aactatttcc ccgcctgcac gagcgtcgct
      961 accagcgcgc caagactttg tcgggcggtg aacaacaaat gttagcgatc ggacgcgcgc
     1021 tgatgacaaa cccggattgc ctaatcatgg atgagccgtc cgaaggactc gcgccgatca
     1081 tcatccaggg cctttgggaa gcgatcggca cgctgaagaa agagggcctg tccattttgc
     1141 tggtcgaaca aaatgcccac ctggctctca aactagtcga ctatgtgcac gtgatgagca
     1201 aaggccaggt ggtctactcc gccaagcccg aggaattgcg cgccaacgac gagatcaagt
     1261 ccagttacct cggcatctag cggctcatcg tcacacgctg gaatcgtgca attagactac
     1321 ccatcacagc aagtgggcac actgggcgcc gacaccacga ccgggcttta catcatagaa
     1381 cgccttgcgg atgatggacg taacttggtc caccgcagtg gggcacggcg gtcggtggtg
     1441 cgttacttgg gcctgacggc ctgcctaaaa tgcgactaag gccgtttaaa atagagcccc
     1501 ggcaggggcg tagagggagg aacgcaatgt ctcgcaaggt cacccgtcgt aaatttatgg
     1561 ccggtacagc cgctggcgcg gttgcggcaa ctttcaaatt cccggccccg gccattgcgc
     1621 aggcggcgcc gttcaagctc ggcctgctca cagtaaagac cggcccgctc gcgcagggcg
     1681 gcatccagat ggaacagggc atcgttacct acctgaagga gaagaactac accttcgccg
     1741 gccgcaaggt tgaattcatc tccgccgata ccggcggtaa cccggcgggc accaagacca
     1801 aggcgcagga gctgatcgag cgcgacaagg tggacaccat tgttgggccg ctagctgcat
     1861 tcgagctgct cgccatcacc gattacatcg cgcagcagaa gacgccgctg ctcagccttg
     1921 ccgccgccga ggatatgacg caacgccgcc cgaatccgta tttcctgcgc gcatcggcga
     1981 cctccgctca agcgatgcat ccgatggcgg agttcgccgc caaggaattg aagctcaagc
     2041 gtatgatcac gatctcggag gacttcgcct tcggctatga acagatgggc ggcttccagc
     2101 aaactttcga ggaaaatggc ggcaagatcc tgaacaagtt gtggccgccg atcgtcactc
     2161 ccgactacac gccctatctg gcgcaaattc aggattgcga cggcgtgtgc cagggattcg
     2221 ccgg
//
LOCUS       DBNGZE010000087         3776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1122576, whole genome shotgun sequence.
ACCESSION   DBNGZE010000087 DBNGZE010000000
VERSION     DBNGZE010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1122576"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..680)
                     /locus_tag="AC16FV_02200"
     CDS             complement(102..680)
                     /locus_tag="AC16FV_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR2848906.1"
                     /translation="MRARLLVFTVILGGFALIQACAPVIVAGGATAAVATTDRRSVGT
                     QLDDKNIELTARRKHNDDKRLGDDVHVNITCFNGTMLLTGEATTAEQRDIVVSLVRTL
                     QGVKRVINEISVTEPTVFASRTNDGWITGRVKTKLLADEKIPGRQVKVVTENGVVYLL
                     GLVTQKEGELAAEATRTVDGIKRVVKLFEYTD"
     gene            complement(682..1308)
                     /locus_tag="AC16FV_02205"
     CDS             complement(682..1308)
                     /locus_tag="AC16FV_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HIR2848907.1"
                     /translation="MAKSNLQTLPDSTTLVTRVKNNFQESILTTRNCMEDLAPIIAQA
                     ATHMIHALLNDRKILICGNGGSAADAQHFSAELLNRFERERPGLPAIALTTDTSTITA
                     IGNDYHFDDIFSKQVRALGQAGDVLLGISTSGNSRNVIRAIEAAHERGIVCVALNGRE
                     GGEITGLLAGHDVNICVPGSSTARIQEVHGIVIHCLCDLIDYQLLGQG"
     gene            complement(1321..1686)
                     /locus_tag="AC16FV_02210"
     CDS             complement(1321..1686)
                     /locus_tag="AC16FV_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR2848908.1"
                     /translation="MPNSRTNLNRGREAEERACRHLQQQGLTLTQRNYRSPFGEIDLV
                     MQERDTLVFVEVRYRARGDFGSAAETVDARKQARLRATAEHYRQNTPRASKKACRFDI
                     VAITGDGEDADFRWLHDVF"
     gene            complement(1689..3629)
                     /locus_tag="AC16FV_02215"
     CDS             complement(1689..3629)
                     /locus_tag="AC16FV_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR2848909.1"
                     /translation="MKPIRWLAIPIICALILAGCETVPVAPTALPPVSTDTAEKAERD
                     GEYLLAAREYTRLAETAESPRKQDFELRAVAALLKAGQLREARSRVELINVTSLDTSF
                     AARKRILQARALIIESAHEKGIRQLDEASRLRNLSPALLSEISEVRAQAELALDNPVG
                     AVRNLIAREQYIVGKEAVAENQQQLWKILVSMPRARLATELNMARDRVLSGWLELALI
                     AVDNVGQSLRMNAAVEQWKKAHPTHPAGEAVLGALISQAPSMIGRVDRIALLLPLSSA
                     YATAAQAVRDGFLAMDAANTDPDKPKIKIYDTGPDPLKTPDIYEQAINDGAQFIVGPL
                     GREAAETIMRQASIRAPTLMLSHTDDEPGSTSKYLFQFGLPPEQEARQVAERAWLDGH
                     RQAAVLYPKTPWGERMMTAWSSHWQRLGGVVVSSQSYSEEDVDFSEPIKILLNITSSE
                     IRKEHIEKTIGQKLRFEARARQDIDYIFLAADAKHGRLIKPQLNFYHAARVPVYSTSH
                     IFTGTGDAMRDIDLDGIQFGDMPWMLVGDGKIQELRQALQRDWPYVHTDLDRLYALGV
                     DSYAVIPHLNRISMDTGARFNGVTSGLSVDHNGRLQRQLLWARFSRGVPRLVDTFLKH
                     QDQFKIQSNDETNERGARVGPP"
     gene            3679..>3776
                     /locus_tag="AC16FV_02220"
     CDS             3679..>3776
                     /locus_tag="AC16FV_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR2848910.1"
                     /translation="MSNAGTLYVVATPIGNLEDMAPRAVRILSEVDL"
BASE COUNT          629 a         1132 c         1238 g          777 t
ORIGIN      
        1 gatatcgaag cagccgttgg tgaagacaat ggggcgggcg agcagcgcca gccgtgccgt
       61 gagttgcgcg gtgtcacgaa cgatcttggc ttcgctgccg gtcaatccgt gtactcgaac
      121 agcttgacga cgcgcttgat gccgtcgacg gtgcgcgtgg cctcggccgc gagttcgcct
      181 tccttctgcg ttaccagtcc caggagatag accaccccgt tttccgtgac caccttcacc
      241 tggcgccccg ggattttttc atcggcgaga agtttggttt tgacccggcc ggtgatccag
      301 ccatcgttgg tgcggctggc gaagaccgtg ggttcggtga cgctgatctc gttgatgacg
      361 cgcttcacgc cctggagggt acgcaccagc gacaccacga tgtcacgctg ctcggccgtg
      421 gtggcctcgc ccgtcagcag catggtgccg ttgaaacaag tgatgttgac gtggacgtcg
      481 tcgccgagcc gcttgtcgtc gttgtgcttc ctgcgcgccg tgagctcgat gtttttatcg
      541 tcgagctggg taccgacgct gcgccggtcg gtcgtagcca ccgccgcggt ggcgccaccg
      601 gccacgatga ccggcgcgca ggcctgtatg agcgcgaagc cgccgagaat gacagtgaag
      661 acgagaagcc gggcgcgcat ctcagccctg tccgagcagt tgatagtcga tcaggtcgca
      721 caggcagtgg atgacgatgc cgtgcacttc ctggatgcgc gccgtggacg aacccggcac
      781 gcagatattg acgtcgtgac cggcgagcag gccggtgatc tcaccgccct cgcgtccgtt
      841 cagcgcgaca cacacgatgc cgcgctcatg tgccgcctcg atggcgcgga tgacgttgcg
      901 tgaattgccc gaggtcgaga tgccgagcag tacgtcgccg gcctggccga gcgcgcgcac
      961 ctgcttggag aagatgtcgt cgaagtggta gtcgttgccg atggcagtga tggtcgaggt
     1021 gtcggtggta agtgcaatcg ccggcagtcc cgggcgttcg cgctcgaagc ggttcagcag
     1081 ttcagcggaa aagtgctgtg catccgccgc cgagccgccg ttgccgcaga tgagaatttt
     1141 tcgatcgttt aacaatgcat gaatcatgtg cgtggcggcc tgcgcgatga tcggcgccag
     1201 gtcttccatg caattgcgcg tggtcaggat gctttcctga aaattgttct tcacgcgcgt
     1261 gacgagcgtg gtggaatcgg ggagtgtctg caaattgctc ttggccatgg gcatcctggg
     1321 tcagaatacg tcgtgtaacc agcgaaagtc agcgtcctcg ccgtcgccgg tgatcgctac
     1381 gatatcaaat cggcaggctt tcttggaggc ccgcggcgtg ttttgccggt aatgttcggc
     1441 ggtggcgcgc aggcgcgcct gcttgcgcgc gtccaccgtt tcggcggcgg agccgaaatc
     1501 gccgcgggca cggtatcgga cctcgacaaa taccagggta tcgcgctcct gcatgacgag
     1561 atcgatctcc ccaaagggac tgcggtaatt gcgctgcgtg agcgtcagtc cttgttgctg
     1621 caggtggcgg caggcgcgct cctccgcctc acgaccgcgg ttgagatttg tccgggaatt
     1681 cggcatcact aaggaggccc gactcgcgca ccgcgctcgt tcgtctcgtc attagattgg
     1741 atcttgaact ggtcctgatg cttgaggaag gtatcgacca gccgcggcac gccgcggctg
     1801 aaacgcgccc acagcagttg ccgctgcagg cggccgttgt gatcgaccga gagtccgctg
     1861 gtgacaccgt tgaagcgggc gccggtgtcc atgctgatgc ggttcaggtg cggaatcacg
     1921 gcatagctgt ccacgccgag ggcgtacagg cggtcgaggt cggtatggac gtagggccag
     1981 tcacgttgca gcgcctgtcg caattcctgg atttttccgt cgccgaccag catccacggc
     2041 atgtcaccga actggatgcc gtcgaggtcg atgtcgcgca tggcatcccc tgtgccggtg
     2101 aagatgtgtg aggtcgagta taccggcacg cgcgcggcat gatagaaatt aagctgcggc
     2161 ttgatcagcc gtccgtgctt ggcgtcggcg gcgagaaaaa tgtaatcgat gtcctgccgc
     2221 gcgcgcgcct cgaagcgcag tttctgcccg atggtcttct cgatatgttc cttgcgtatt
     2281 tcgctgctcg tgatgttgag cagaatcttg atcggctcgg aaaagtcgac gtcttcctcg
     2341 ctgtaggact ggctggagac caccacgccg ccgagccgtt gccagtgact cgaccacgcc
     2401 gtcatcatgc gctcgcccca cggcgttttc ggataaagca ccgccgcctg ccgatgcccg
     2461 tcgagccagg cgcgctcggc cacctgccgt gcctcctgtt ccggcggcag gccgaactgg
     2521 aaaagatatt tcgatgtcga gcccggctcg tcgtcggtat ggctcagcat cagggtcggc
     2581 gcccggatgc tggcctggcg catgatggtt tccgcggcct cgcgcccgag ggggccgaca
     2641 ataaactgtg caccatcgtt gatggcctgc tcgtagatgt caggcgtctt cagcgggtcc
     2701 ggtccggtat cgtagatttt gatcttgggt ttgtccggat cggtattggc ggcgtccatg
     2761 gccaggaatc cgtcgcgcac cgcctgcgcc gcggtggcgt aagcggaaga caacggcagc
     2821 agcaaggcaa tgcggtcgac gcggccgatc atgctgggtg cctggctgat cagcgcgccg
     2881 agcaccgctt ctcccgcagg gtgagtcgga tgagcttttt tccattgttc cacggcggcg
     2941 ttcatgcgca acgactggcc gacgttgtcc accgcgatca gggccagttc cagccagccg
     3001 gacaacaccc ggtcgcgcgc catgttgagt tcagtggcga gccgcgcgcg cggcatcgat
     3061 accaggattt tccacaattg ctgttgattc tcggcgacgg cctccttgcc gacgatgtat
     3121 tgctcgcggg cgatgagatt gcgcaccgcg cccacgggat tgtcgagcgc gagttccgcc
     3181 tgtgcgcgca cctcgctgat ctcggaaagc agcgccgggc tcaggttgcg caggcgactg
     3241 gcctcgtcca gctggcgtat gcctttttca tgcgcgcttt cgataatcag cgcgcgtgcc
     3301 tgcaaaatcc gtttgcgcgc agcgaacgag gtatccagcg acgtgacgtt gatcagctcg
     3361 acgcgcgaac gcgcctcccg cagttgtccg gccttgagca gagcggccac ggcgcgcagt
     3421 tcaaagtcct gcttccgggg cgattcggcg gtttcggcca ggcgcgtgta ttcgcgcgcg
     3481 gccagcaggt attcaccatc acgctcggcc ttttccgcgg tgtccgtgct gaccggcggc
     3541 aaggccgtgg gcgccaccgg cacggtctcg caaccggcga ggatcagcgc acatattata
     3601 ggtatggcca gccagcggat gggtttcatt cagtttcctg tttagaatgc gcgaaagtat
     3661 cgaatcgggg aaaaattagt gtcaaacgcc ggcacgctct acgttgtggc cacgccgatc
     3721 ggcaacctcg aagacatggc gccgcgcgcg gtgcggatcc tgtcggaggt cgatct
//
LOCUS       DBNGZE010000088         6595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_845238, whole genome shotgun sequence.
ACCESSION   DBNGZE010000088 DBNGZE010000000
VERSION     DBNGZE010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_845238"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..179
                     /locus_tag="AC16FV_02225"
     CDS             6..179
                     /locus_tag="AC16FV_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HIR2848911.1"
                     /translation="MARGFTPVVTRSAHQLMHPKFSRTIADYLARERLDVDSHVNELN
                     EHVPFKKQSTAGK"
     gene            184..918
                     /gene="aat"
                     /locus_tag="AC16FV_02230"
     CDS             184..918
                     /gene="aat"
                     /locus_tag="AC16FV_02230"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR2848912.1"
                     /translation="MFVLKPGSADLRFPPVELASPEGLLAVGGDLRAERLLEAYRHGI
                     FPWYNPGQPILWWSPDPRAVLFPAKLRVSRSLGKTLRRKKFEVRLDTAFRDVMQRCAE
                     PRDNRPGHGTWITPEMIEAYCVLHERGLSHSVESWSDGKLVGGLYGVALGRAFFGESM
                     FSRETDASKVAFVQLVRQLELWGFAFVDCQLPSAHLFSLGAEEIRRRDFLDRLERALQ
                     QPGRPGRWRFEADPAILTGSHPPARP"
     gene            915..1649
                     /locus_tag="AC16FV_02235"
     CDS             915..1649
                     /locus_tag="AC16FV_02235"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR2848913.1"
                     /translation="MTSSHKPPEIFLSMPHACSYLPGRTATSLFLDPRQPLDSRQYAG
                     FMRLGFRRSGDLIYRPHCRACNACIPVRIPVNRFRADRGQRRIWKRNQDLSVIACPPA
                     FEEEHFELYQRYQSARHPGGGMDDPDPQKYLNFLGSRHIHTVFFEFRLKRRLLGVAVT
                     DILPDGLSAVYTFFDPDEKRRALGVHAVLWQIERARGQQLPWLYLGYWIRECAKMSYK
                     GHYRPLEAFLQGHWVQLDNPDTHTSP"
     gene            1726..1980
                     /locus_tag="AC16FV_02240"
     CDS             1726..1980
                     /locus_tag="AC16FV_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848914.1"
                     /translation="MLSFLYRLVEHYHREHGLHPNLLYLNQWHYQKLLECLPEMEGQE
                     EVSRFLMMQIVITPEAVHPYVAWLPSSVGAGRARQQERAL"
     gene            2084..2302
                     /gene="infA"
                     /locus_tag="AC16FV_02245"
     CDS             2084..2302
                     /gene="infA"
                     /locus_tag="AC16FV_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR2848915.1"
                     /translation="MAKEEHIEMEGTITETLPNTLFRVKLDNGHVVTAHISGKMRKNY
                     IRILTGDRVTVQLTPYDLTKGRITFRAR"
     gene            complement(2371..4635)
                     /gene="clpA"
                     /locus_tag="AC16FV_02250"
     CDS             complement(2371..4635)
                     /gene="clpA"
                     /locus_tag="AC16FV_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR2848916.1"
                     /translation="MLSQELEFSLNSAFQSAREKRHEFITVEHLLAALLDNPSAARVL
                     RACGGNIEELRRSLSAFLEENVPKLAAGSKIDTQPTIGFQRVIQRAVLHVQGVGKKEV
                     TGANVMIAIFGEKESHAVYFLHKQNISRFDVVNYVSHGISKVPGENQNELPATEENNE
                     EGAASEKSPLDVFCINLNEQARVGKIDPLIGRSKEVERTIQVLCRRRKNNPLYVGEAG
                     VGKTAIAEGLAKKIVDGEVPDVLMHSTIYALDMGSLLAGTKYRGDFEKRLKAVLVQLK
                     KHENAVLFIDEIHTIIGAGAASGGAMDASNLLKPVLASGDLKCIGSTTYQEYRNIFEK
                     DRALSRRFQKIDVTEPSVEEAVEILRGLKNRFENHHGVRYTQQALRSAVELSHRYIND
                     RHLPDKAIDVIDEAGASQHLLPPSRRKKTIGVHDIEDIVAKIARIPPKTVSTSDREAL
                     KTLERDLKMVVFGQDDAIEALATAIKMSRSGLGHPEKPVGSYLFAGPTGVGKTEVTRQ
                     LARILGLELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAIVKHPHSVLLMDE
                     IEKAHPDVFNLLLQVMDHGTLTDNNGRKADFRNVILVMTTNAGAERLSRSSIGFTKQD
                     HAGDELQEIRRMFSPEFRNRLDATIQFKPLDETTISHVVDKFVLELEAQLADKHVTVE
                     LDNAARAWLAKHGHDPAMGARPMARLIQEKVKKPLANELLFGKLSEGGHVKVSADDKG
                     LVFDFIPATDKTPA"
     gene            complement(4695..5012)
                     /gene="clpS"
                     /locus_tag="AC16FV_02255"
     CDS             complement(4695..5012)
                     /gene="clpS"
                     /locus_tag="AC16FV_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR2848917.1"
                     /translation="MSDKPQHYLDGPVVQEAKPRLQKPQLYKVILLNDDYTPMEFVVI
                     ILERFFAKNREDATRIMLHVHQKGIGICGVYIREIAETKVRQVLAFAQEHQHPLQCTM
                     EPE"
     gene            5311..5520
                     /locus_tag="AC16FV_02260"
     CDS             5311..5520
                     /locus_tag="AC16FV_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2848918.1"
                     /translation="MPLGTVKWFNASKGYGFITPTDGGDDVFAHYSAIEMEGYKTLKE
                     GQQVEFEVQEGPKGPQASHIRSLSK"
     gene            complement(5575..>6595)
                     /gene="icd"
                     /locus_tag="AC16FV_02265"
     CDS             complement(5575..>6595)
                     /gene="icd"
                     /locus_tag="AC16FV_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR2848919.1"
                     /translation="YGNNTWLPAETFQAVKEYVVSIKGPLTTPVGGGIRSLNVAMRQE
                     LDLYVCLRPVQYFKGVPSPVKEPEKTDMVIFRENSEDIYAGIEWPSGSPEAQKLIAFL
                     QREMGVKKIRFPETSAIGIKPVSREGTERLVRRAIQYAIANDCPSVTLVHKGNIMNFT
                     EGGFKSWGYELAQREFGAQLIDQGPWMHMNNPKSGKEVIIKDVIADAFLQQILLRPAE
                     YSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDTVAMFEATHGTAPKYAGKDMVNP
                     GSLILSAEMMLRHLGWVEAADLILKGMEGAISARTVTYDFARLMPGATQVSCSGFGDA
                     IIRCM"
BASE COUNT         1228 a         2059 c         1931 g         1377 t
ORIGIN      
        1 acaagctggc gcgcggtttc acgccggtgg tgacgcgctc ggcgcaccag ctgatgcatc
       61 cgaaattcag ccgtaccatc gcggattatc tggcgcggga acgacttgac gtggatagtc
      121 acgtcaatga actgaacgag cacgtgccgt tcaaaaagca atcgacggca ggaaaatagc
      181 gcaatgttcg tcctcaaacc cggttcggct gacctgcgat ttcctccggt ggagctggct
      241 tcgcccgagg gcctgctggc cgtgggcggc gacctgcgcg ccgagcggtt gctggaagcc
      301 taccgtcacg gcattttccc ctggtataac ccgggacaac cgatcctgtg gtggtcgccc
      361 gacccacgcg ctgtgctgtt cccggcgaaa ctgcgcgtct cgcgcagcct cggcaaaacc
      421 ctgcgccgga aaaaattcga ggtgagactg gacaccgcct tccgcgacgt catgcaacgc
      481 tgcgccgagc cacgggataa tcgccccggc cacggcacct ggatcacgcc ggagatgatc
      541 gaggcctatt gcgtactgca tgaacgcgga ttgtcgcatt ccgtggaatc ctggtcggac
      601 ggaaaactcg tcggtgggct ttatggcgtc gcgcttggcc gcgccttttt cggcgagtcc
      661 atgttctcgc gcgaaaccga cgcctccaag gtggcgttcg tgcaactcgt gcggcaactc
      721 gaactctggg gctttgcctt tgtcgactgc cagctgcctt cggcgcatct cttcagtctc
      781 ggcgcggagg aaatccgccg ccgcgatttc ctggatcggc tggaacgggc gctgcaacaa
      841 ccaggtcgcc cgggacgctg gcgctttgag gcggatccgg caatacttac ggggagtcat
      901 ccaccggcca ggccatgacc agctcgcaca aaccgccgga aatttttctc tccatgccgc
      961 atgcgtgcag ctatctgccc gggcgcaccg cgacctcgct gtttctcgat ccgcgtcaac
     1021 cgctcgacag tcgccagtac gccggcttca tgcgcctcgg tttccggcgc agcggcgatc
     1081 tcatctaccg gccgcattgc cgcgcgtgca acgcctgcat tccggtgcgc atcccggtga
     1141 atcgtttccg ggccgatcgc ggacagcggc gcatctggaa acgcaatcag gacctgtcgg
     1201 tgatcgcgtg cccgccggcc ttcgaggaag aacatttcga gctctaccag cgttatcagt
     1261 ccgcgcgtca tccgggtggc ggcatggacg atcccgatcc gcagaaatac ctgaacttcc
     1321 tcggctcgcg tcacatccat accgtcttct ttgaatttcg gctaaagcga agacttctgg
     1381 gcgtcgccgt gaccgatatc cttccagacg ggctgtcggc ggtgtatacc tttttcgatc
     1441 cggacgaaaa acgccgcgcc ctcggcgtcc atgccgtgct gtggcagatc gaacgcgccc
     1501 gcgggcaaca gttgccgtgg ctctacctgg gttactggat cagggaatgc gcgaaaatgt
     1561 cctataaggg ccattaccgc ccgctggaag cctttcttca gggccactgg gttcagctcg
     1621 acaatcccga cacgcataca agcccgtaaa tcgccgccct cggcacgtct attgcatctc
     1681 tttcggcata ggccgggctc cccacccggc cggggtgcga aaaccatgct cagctttctc
     1741 tatcgtctcg tcgagcacta tcaccgcgaa cacggtcttc atccgaacct gctctacctg
     1801 aaccagtggc attaccagaa actgctggaa tgccttccgg aaatggaggg acaggaggaa
     1861 gtctcccggt ttctgatgat gcagatcgtc atcacccccg aggccgtgca tccctacgtg
     1921 gcctggttgc cgagcagcgt cggcgccggc cgcgcgcgcc agcaggaacg cgcgctctga
     1981 ccttcccttc ggcggcgatc tggccggatc gccgcccaaa accgctttac gcccacacgc
     2041 gactgcggca tcatacgccg cgaattcaac cttgggttaa cgcgtggcga aagaagagca
     2101 tatcgaaatg gaagggacca tcaccgaaac ccttcccaac accctgttcc gggtcaagct
     2161 ggacaacgga cacgtggtaa cggcgcatat ctccggcaag atgcgcaaaa attatatccg
     2221 catcctcacc ggcgaccgcg tcaccgtgca acttaccccc tacgatctca ccaagggccg
     2281 gatcaccttc cgcgcccgtt agctgccgat tcctgaataa aaaacgccgg cgcatttgca
     2341 tgcgccggcg ttgttgttac gactggagaa tcaggccggg gtcttgtcgg tggcggggat
     2401 gaagtcgaac accaggccct tgtcgtccgc gctcaccttg acgtgcccgc cctccgaaag
     2461 cttgccgaag agcagctcgt tggcgagcgg cttcttgacc ttctcctgga tcagccgcgc
     2521 catcgggcgc gcgcccatgg ccggatcgtg cccatgtttg gcgagccagg cacgcgcagc
     2581 attgtccagt tcgaccgtga catgcttgtc agccaactgc gcctccagct cgagaacgaa
     2641 cttgtccacc acatgcgaga tggtcgtctc gtccagcggc ttgaattgaa tggtcgcatc
     2701 caggcgattg cggaactcgg ggctgaacat gcgccgaatc tcctgcaatt cgtcaccggc
     2761 atggtcctgc ttggtgaaac cgatgctcga ccggctcagg cgctcggcgc cggcgttggt
     2821 cgtcatcacc aggatgacat tgcggaaatc cgccttgcgt ccgttgttgt ccgtgagcgt
     2881 gccgtggtcc atgacctgca acagcaggtt gaacacatcc gggtgcgcct tctcgatctc
     2941 gtccatgagc agcaccgaat gcggatgctt gacgatggcc tcggtcagca ggccgccctg
     3001 atcgaaaccg acgtagcccg gcggcgcgcc gatgaggcgc gacacggtat gccgctccat
     3061 gtattccgac atgtcgaaac gaatgagttc gagcccgagg atgcgcgcca gctgccgcgt
     3121 gacctcggtc ttgccgacgc ccgtgggtcc ggcgaacaga taggagccga ccggcttttc
     3181 cggatgaccc aggccggagc gcgacatctt aatcgcggtc gcgagcgcct cgatggcgtc
     3241 gtcctgcccg aacaccacca tcttgaggtc gcgttcgagc gtcttgaggg cttcgcgatc
     3301 cgacgtggaa accgtcttgg gcggaatgcg cgcgatcttg gcgacgatgt cctcgatgtc
     3361 gtgcacgccg atggttttct tgcggcggct cggcggcagc agatgctgac tggcgccggc
     3421 ctcgtcgatc acgtcgatgg ccttgtccgg caggtgccga tcgttgatat agcggtgcga
     3481 cagctccacc gcgctgcgca gcgcctgctg cgtgtaacgc acgccatggt gattctcaaa
     3541 gcggttcttc aggccgcgca ggatttccac cgcctcctcg accgacggtt cggtgacgtc
     3601 gatcttctgg aaacggcgcg acagcgcacg gtctttctcg aagatgttgc ggtattcctg
     3661 ataggtggtc gaaccgatgc acttgagatc gcccgaggcc agtaccggct tcagcagatt
     3721 cgaggcatcc atggcgccgc ccgacgccgc gcccgcaccg atgatggtgt ggatctcgtc
     3781 gatgaacagc accgcgtttt catgtttctt gagttgcacc agcaccgcct tcaggcgctt
     3841 ctcgaaatcg ccgcggtact tcgtgcctgc cagcagcgaa cccatgtcga gtgcgtagat
     3901 ggtgctgtgc atcagcacgt ccgggacttc gccgtccacg atcttcttgg ccagcccttc
     3961 ggcgatggcg gtcttgccca cgccagcctc gcccacgtac agcggattgt tcttgcgccg
     4021 gcggcacaac acctgaatgg tgcgctccac ttccttgctg cgtccgatca gcggatcgat
     4081 cttgcccacc cgcgcctgtt cgttgaggtt gatgcaaaac acgtcgagtg ggctcttttc
     4141 cgaagcggcg ccctcctcgt tgttttcctc ggtggcggga agttcgttct ggttctcgcc
     4201 cggcaccttg gaaatgccgt gcgacacata gttgaccacg tcgaagcggc tgatgttctg
     4261 cttgtgcagg aaataaacgg cgtgcgactc cttctcgccg aaaatcgcga tcatgacatt
     4321 ggcgccagtg acttccttct tgcccacgcc ctggacgtgc agcaccgcgc gctggatcac
     4381 gcgctgaaaa ccgatggtcg gctgggtgtc gatcttgctg cccgcggcga gcttggggac
     4441 gttttcctcg aggaaggcgc tcaggctacg gcgcagctcc tcgatgttgc caccacaggc
     4501 acgcagcacg cgcgcggcgg agggattgtc gagtagcgcc gccagcagat gctccacggt
     4561 gatgaattcg tggcgctttt cacgtgcgct ctggaacgcg ctgttcagtg agaattcaag
     4621 ttcttgcgac aacatggcac cgcctccggg cactggcccc tcaacacaaa tattcccttt
     4681 tgaaagtctt ccccttattc cggttccatg gtgcactgca acgggtgctg gtgttcctgc
     4741 gcaaatgcca gcacctgacg caccttggtt tccgcaatct cacggatgta aaccccgcat
     4801 attcctatac ccttctgatg cacatgcagc atgatgcggg tcgcgtcttc cctgttcttg
     4861 gcgaaaaacc gttccagaat aattacgacg aactccattg gagtatagtc gtcgttcagc
     4921 aggatgactt tgtacagctg cggcttctgg agccggggct tcgcctcctg gacgaccgga
     4981 ccgtcgagat agtgttgagg cttgtcgctc atatataaaa ggttacacca attgctcgcg
     5041 gaacccgcgt cccgtctggc gaattcaagt acattgtgcg acccgtgccc gaaatttcaa
     5101 cccctgaaaa tatggcatga aatttgaggc tgttccggcg tttcgggaac cgccggccgg
     5161 cgtgacaacg ccacggaggg gtttgcttga cttgcctgca aaaaaagaag gatgatggct
     5221 gggcgcgcct ttctcaggca cttgcaggag gcgggtgcga tatcagcaac ctgaaggata
     5281 gcggcgtttg ccgcagcagg agggggatgt atgccactag gtactgtgaa gtggtttaac
     5341 gccagcaagg gttatggatt catcacaccc acggatggcg gcgatgatgt cttcgcgcat
     5401 tattccgcaa tagaaatgga agggtacaag actctcaaag agggtcagca agtcgagttt
     5461 gaagttcagg aaggtcccaa gggcccccag gcttcccaca tacgttccct atccaagtaa
     5521 tcggcgttac taccgtttga agtttacgac ccggccaagc gccgggttta ttttttacat
     5581 acaacgaatg atggcatccc cgaagccaga gcaactcacc tgggtagcac cgggcatgag
     5641 tcgcgcgaaa tcgtaagtca ccgtcctggc ggaaatcgcc ccttccattc ccttgagaat
     5701 cagatcggcg gcttccaccc agcccaggtg acgcaacatc atctcggccg acaggatcag
     5761 agagccggga ttgaccatgt ccttgccggc gtacttcggt gcggtgccgt gcgtcgcctc
     5821 gaacatcgcc acggtgtcag acaggttcgc gcccggcgca atgccaatgc cgccgacctg
     5881 cgccgcgagc gcatcggaga tgtaatcgcc gttgagattg agcgtagcga tcacgctgta
     5941 ttccgccggg cgcaacagga tctgttgcag aaaggcgtcg gcgatcacat ccttgataat
     6001 gacttccttt ccggatttcg ggttgttcat gtgcatccat ggcccctggt cgataagctg
     6061 cgcgccgaat tcgcgctgcg ccagttcata gccccagctc ttgaatccgc cttcggtgaa
     6121 gttcatgatg ttgcccttgt gcaccagggt cacggagggg cagtcgttgg cgatggcgta
     6181 ctggatggcg cggcgcacca gccgttccgt gccctcgcgc gagaccggct tgatgccgat
     6241 ggcggaggtt tccggaaagc gtattttctt cacgcccatt tcgcgctgca ggaaggcgat
     6301 cagcttctgc gcctcgggcg aaccggacgg ccactcgatg ccggcgtaga tgtcttccga
     6361 gttttcccgg aagatcacca tgtcggtttt ctcgggttct ttcacgggac tcgggacgcc
     6421 cttgaagtac tgcaccgggc gcaggcagac gtacaggtcc agctcctggc gcatcgcgac
     6481 gttcagcgag cggatgccgc cgcccaccgg cgtcgtcagc ggtcccttga tcgagacgac
     6541 atattccttg accgcctgaa aagtttccgc cggcagccag gtattattgc cgtag
//
LOCUS       DBNGZE010000089         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1401268, whole genome shotgun sequence.
ACCESSION   DBNGZE010000089 DBNGZE010000000
VERSION     DBNGZE010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1401268"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16FV_02270"
     CDS             <1..368
                     /locus_tag="AC16FV_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848920.1"
                     /translation="PVMLLYKLLGNRPSFFVYVGMVAGFLGGFAALWAYVVVPIQYYY
                     QIWGWLGIVGGIIAVILVPAQLVLFLVVAFLKGGAAVYLGNFFAGICFGLAGLLLFNS
                     AFSMSIWSYFLKKKRSEAV"
     gene            complement(426..1196)
                     /locus_tag="AC16FV_02275"
     CDS             complement(426..1196)
                     /locus_tag="AC16FV_02275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR2848921.1"
                     /translation="MILPHSPTPFSPANCPRTDLVALRSLLFNVVMLVSVAIYAPLML
                     PTALLPYPLRYRLVSQWARFQVFMLKTLCGLDYRVEGREHLPQGAAIVMSKHQSAWET
                     IVFQQIFPPQTWVLKHELMWIPLFGWALALMRPIAIDRGAGRKAVEQVIAQGRERLQS
                     GIWVVVFPEGTRVAPGTRKRYAMGGAVLAAETGYPVVPVAHNAGSFWLRRGFMKKPGT
                     VRVVIGPMIDPRGQKAEEIIRQTEEWIESRMVELERVV"
     gene            complement(1141..1692)
                     /gene="gmhB"
                     /locus_tag="AC16FV_02280"
     CDS             complement(1141..1692)
                     /gene="gmhB"
                     /locus_tag="AC16FV_02280"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR2848922.1"
                     /translation="MKLVILDRDGVINHDSDDYIKSPEEWVPIPGSLEAIARLHREGY
                     KVIVATNQSGVGRGLFDMETLGRIHARMLEAVRAKGGEIDAIFFCPHKPEDNCSCRKP
                     QPGLFHEIAERLKVNLNGVYAVGDSERDVAAARLVSARPVLVRTGKGKRTLKKSKALA
                     DVPVFDDLAAFTDAFLTGKLPAN"
     gene            complement(1828..2157)
                     /locus_tag="AC16FV_02285"
     CDS             complement(1828..2157)
                     /locus_tag="AC16FV_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HIR2848923.1"
                     /translation="MQKDSYQDMLATVQAFHDKHRFKETGGEELAYRVALMTEELGEI
                     SACVTKGKSKEHLAEECADLFILLLGTAIAADFDLRQAFWDKMQKVQSRESRMINGRI
                     RVSEFKE"
     gene            complement(2167..>2950)
                     /locus_tag="AC16FV_02290"
     CDS             complement(2167..>2950)
                     /locus_tag="AC16FV_02290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR2848924.1"
                     /translation="EQHYWPRTAGGELPKQPVAVCVALADKLDTLVGIYGIGLVPTGE
                     KDPFGLRRAALGVVRILVEKSLALDVKDLLASARSQFPNGVIADSVAQDLHGFMLERL
                     KSYLKEKGFEPDEIDAVVSLNPARLDQVLPRLKALKEFRALPEGQALAAANKRIRNIL
                     RQAGGTPSDKVDAGRLTEAAERKLSESVQTLEAQVAPLFRVGNYAEALKHLAGLRPAV
                     DEFFDKVMVMVDDTAVRDNRLALLNRLGNLFLNVADISRLQS"
BASE COUNT          543 a          933 c          854 g          620 t
ORIGIN      
        1 tgccagtaat gctgttatac aagttgctcg ggaaccgtcc atcattcttt gtatatgttg
       61 gaatggtcgc gggttttttg ggcggtttcg ctgccctatg ggcgtatgtt gtcgttccaa
      121 ttcagtatta ctatcaaatc tggggatggc ttggaattgt aggcggcatc atcgccgtga
      181 tattggtgcc tgcccaattg gttctgttcc ttgtagtcgc atttcttaag ggtggcgcag
      241 ccgtatactt gggcaatttc tttgcaggta tctgttttgg tttagctggg ttgctcctct
      301 ttaatagcgc gttctccatg tcaatttgga gctatttcct gaaaaagaaa cggagtgagg
      361 cggtataaca cgtcgctcaa cctgactcgc ggtgctgacg caccgcgagt caggttagct
      421 ctgcgttaaa caacccgctc caactccacc atcctgcttt cgatccactc ctccgtctgc
      481 cttatgattt cctcggcctt ctggccacgc gggtcaatca tggggccgat gaccacgcgc
      541 acggtgccgg gttttttcat gaaaccgcgc cgcaaccaga acgagccggc gttgtgcgcc
      601 accggcacca ccgggtaacc ggtctcggcc gccaacaccg cgccgcccat ggcgtagcgc
      661 ttgcgcgtcc ccggcgccac gcgcgtacct tccgggaaca ccaccaccca gataccggat
      721 tgcaggcgct cgcgcccctg cgcgatgacc tgctccaccg ccttgcgccc ggcgccgcgg
      781 tcaatggcga tcgggcgcat cagcgccagc gcccagccga acagcggaat ccacatcagc
      841 tcgtgcttga gcacccaggt ctgcggcggg aatatctgct gaaacacgat ggtctcccac
      901 gccgactggt gcttggacat gacgatcgcc gcgccctggg gcaggtgctc gcgcccttcc
      961 acgcggtaat cgaggccgca cagggttttc agcatgaaca cctgaaaccg cgcccactga
     1021 ctgacgagac gatagcgcag cgggtacggt agcagtgcgg tcggcagcat gagcggcgcg
     1081 tagatcgcca ccgacaccag catcacgaca ttgaacagga gggagcggag cgcgaccaag
     1141 tcagttcgcg ggcagtttgc cggtgagaaa ggcgtcggtg aatgcggcaa gatcatcgaa
     1201 taccggcacg tccgcgagcg ccttgctctt cttgagggtg cgtttgccct tgccggtgcg
     1261 caccagcacc ggccgcgccg agaccaggcg cgccgccgcc acgtcgcgct cgctgtcgcc
     1321 aacggcataa acaccgttca ggttcacctt gaggcgttcg gcgatctcat ggaacaggcc
     1381 gggctgtggc ttgcggcaac tgcaattatc ctcgggcttg tgcgggcaga aaaaaatcgc
     1441 gtcgatctcg ccgcccttgg cgcgcaccgc ctcgagcatc ctggcgtgga tgcgtccgag
     1501 cgtttccatg tcgaacagcc cccggccgac gccggactgg ttggtggcga cgatgacctt
     1561 gtaaccctcg cgatgcaggc gcgcgatggc ctcgaggctg cccgggatcg gcacccactc
     1621 ctcgggcgat tttatgtagt cgtccgaatc gtggttgatc acgccgtcgc ggtcgaggat
     1681 gacgagcttc atatgatctt ggaacgcaga taaacgcgga ttattaacgc agataaacgc
     1741 agatgattag aaattgattc gccatctacc cgaatcaatc cgcgttcatc ttcatacata
     1801 tctgcgttca tctgcggttc cagaatatta ttccttgaac tccgacacgc ggatgcggcc
     1861 gttgatcatg cgcgattcgc ggctctgtac cttctgcatc ttgtcccaga acgcctgcct
     1921 gagatcgaaa tccgccgcga tggcggtgcc gagcagcagg atgaacaggt cagcgcattc
     1981 ctcggccaga tgttctttcg actttccctt ggtcacgcag gcggaaatct cgcccagttc
     2041 ttccgtcatg agcgccacgc ggtaggccag ctcctcgccg ccggtctctt tgaagcggtg
     2101 tttgtcgtgg aaggcctgca ccgtcgcgag catgtcctgg taggaatcct tctgcatact
     2161 tcctcctcag ctttgcagcc gcgaaatatc cgcgacgttc agaaacagat tgcccaagcg
     2221 gttgagcagc gccagacgat tatcacgcac ggcggtatcg tccaccatga ccatgacctt
     2281 gtcgaaaaac tcatccaccg ccggacgcag cccagccagg tgcttgagcg cctcggcata
     2341 attgcccacc cggaacaacg gcgccacttg cgcctccagc gtttgtaccg attctgaaag
     2401 cttgcgttcc gctgcctcgg tcagccgtcc cgcgtccacc ttgtcggagg gcgtgccgcc
     2461 ggcctggcgc agaatgttgc ggatgcgctt gttggccgcg gccagcgcct gcccctcggg
     2521 caacgcccgg aattccttga gcgccttcag gcgcggcagc acttggtcga gtcgcgccgg
     2581 gttgagcgac accaccgcgt cgatctcgtc cggctcgaag cccttttcct tcaaataaga
     2641 cttcaggcgt tcgagcatga agccatgcaa atcctgcgcg acggaatcgg caataacgcc
     2701 gttgggaaac tggctgcgcg cgctggcgag aagatctttc acatcgagtg ccagggattt
     2761 ttccaccagg atacgcacca cgcccagcgc cgcgcgccgc aggccgaagg ggtctttctc
     2821 gccggtcggc accagcccga tgccgtaaat gccaactagg gtgtcgagct tgtccgccag
     2881 cgccacgcac actgcgaccg gctgcttcgg cagctcgccg ccggcggtgc gcggccagta
     2941 atgctgctcg
//
LOCUS       DBNGZE010000090         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1817964, whole genome shotgun sequence.
ACCESSION   DBNGZE010000090 DBNGZE010000000
VERSION     DBNGZE010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1817964"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="AC16FV_02295"
     CDS             <1..257
                     /locus_tag="AC16FV_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR2848925.1"
                     /translation="HGERRRAFEQMTREALLARFAERILPVSIEVASSWGAFSGHHKQ
                     TLPVIDGLIAATARVHGLTVVTRNVTDFRRFDVPVFDPWK"
     gene            364..1719
                     /gene="mgtE"
                     /locus_tag="AC16FV_02300"
     CDS             364..1719
                     /gene="mgtE"
                     /locus_tag="AC16FV_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR2848926.1"
                     /translation="MPRARKPKKPDHLKRVLAALELGNTAGVRAILADLHPADIARVL
                     EGLPPEPRATVWAQVDIQHMGEVLLELPEAVRSDLVKLMDGVALVAAARALDTDDIAD
                     LIPDLSDEVIAEILFALDKQDRQRLDAVMSYAEDTAGGLMNVDAITVRENITLEVVLR
                     YLRRRDELPEHTNQLFVVDRDDRVVGILSVAKLLTSDAWMRVSQVMEREVIKFPVLTP
                     DKEVAAAFGNYNLISAPVVDENNHLLGRITVDDVVDVMREQAERERLDRAGLREAEDM
                     FASVWQTVRNRWTWVAINLVTAIIASRVIGAFEGSIEKLVALASLMPIVAGIGGNTGN
                     QTTTMIVRALALGQLSETNMRALFAKEIRVSLVNGLVWGGLLGIMAFALYRNIALGVV
                     MTAAMTLNLMLAASAGVLIPLTRLKLGRDPARGSSVLITAITDSGGFFIFLGLATLFL
                     V"
     gene            1839..2243
                     /locus_tag="AC16FV_02305"
     CDS             1839..2243
                     /locus_tag="AC16FV_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HIR2848927.1"
                     /translation="MLPNTIQLISQAIKEKRCIAIRYHDQRQIRVVEPHAVYTDERGE
                     LVMDGYQTRGHSASGRPPPFWRPFRMNKITAVSVLKETFQPRIAEGFSANRLKYRSGL
                     VAIVQDSQPAFAYVYPAQTTEIGPQLPEGMRR"
     gene            complement(2280..>2909)
                     /locus_tag="AC16FV_02310"
     CDS             complement(2280..>2909)
                     /locus_tag="AC16FV_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR2848928.1"
                     /translation="CLHDTSLFRPQLRAILRASAFGKVRRMIPMLSGQEELFQVLDLI
                     EETKSDLKREHIKFNPKIPIGGMIEVPAAAVSADLFAPHLDFFSIGTNDLIQYTLAID
                     RVDDAVNYLYDPLHPSVLRLISITIQAGRKARIPVAMCGEMAGDPRYTRLLLGLGLSE
                     FSMHPATLLEVKKIVREANLGQLTRFAKDLMKLGDPRELRARVEKQSRH"
BASE COUNT          549 a          911 c          917 g          532 t
ORIGIN      
        1 cgcacggcga gcggcggcgt gccttcgagc agatgacgcg tgaggcgttg ctggcacgtt
       61 tcgccgagcg cattctgccg gtgagcattg aggtggcatc ttcgtggggt gcattctctg
      121 gccatcataa gcagacgcta cccgtgatag atggtctcat cgccgccacg gcccgtgtac
      181 atggcctgac agtggtcacg cgcaatgtaa cggatttccg gcgttttgac gtgccggtat
      241 tcgatccctg gaaatagaca atattcagca acggatcaat caacttctgc ttcacgttgt
      301 gtaattcccc tttgcgtcta tgcgggagtc atctacactc tggccgcgaa aaaaggaatc
      361 cccatgccca gggcgcgcaa acccaagaaa ccggatcatc tgaagcgggt tttggccgcg
      421 cttgagctcg gcaacacggc cggtgtgcgc gcgatcctgg ccgatctgca cccggccgat
      481 atcgcccgcg tcctcgaagg cctgcccccg gagccgcgcg ccaccgtctg ggcgcaggtg
      541 gacatccagc acatgggcga ggtgctgctg gaactgcccg aggcggtgcg cagcgacctg
      601 gtcaagctga tggacggggt ggcgctggtg gcggcggcgc gcgccctcga caccgacgac
      661 atcgccgatc tcatccccga cctgtcggac gaggtgatcg cggaaatcct gttcgcgctg
      721 gataaacagg accgccagcg cctcgacgcg gtaatgtcct acgccgagga cacggccggc
      781 ggcctgatga acgtggatgc gatcacggtg cgcgagaaca tcacgctcga agtcgtgttg
      841 cgctacctgc gccggcgcga cgagctgccg gagcatacca accagctgtt cgtggtggac
      901 cgcgacgatc gcgtggtcgg gatactgtcg gtggccaaac tgctgacctc cgatgcctgg
      961 atgcgtgtct cgcaggtgat ggagcgcgag gtgatcaaat tcccggtgtt gaccccggac
     1021 aaggaggtgg cggcggcgtt cggaaactac aacctgatct cggcgccggt ggtggatgaa
     1081 aacaaccatc tgctcgggcg catcaccgtg gacgacgtcg tggacgtgat gcgcgagcag
     1141 gccgaacgcg aacgcctcga ccgcgcgggt ctgcgcgagg ccgaggacat gttcgccagc
     1201 gtgtggcaga cggtgcgcaa ccgctggacc tgggtcgcca tcaatctggt cactgccatc
     1261 atcgcgtcgc gcgtcatcgg cgccttcgag ggctcgatcg aaaaactggt ggcgctcgct
     1321 tcgctcatgc ccatcgtcgc cggcatcggc ggcaacaccg gcaaccagac caccaccatg
     1381 atcgtgcgcg cgctcgcgct cggccagctc agcgaaacca acatgcgcgc gctgttcgcc
     1441 aaggagatcc gcgtgagcct cgtcaacggc ctggtctggg gcggactgct cggcatcatg
     1501 gcctttgcgc tttaccgcaa catcgccctc ggcgtcgtca tgaccgccgc catgaccctg
     1561 aatctcatgc tggccgccag cgcgggggta ttgatcccgc tgacgcgtct caagctgggg
     1621 cgcgatccgg cgcgcggttc gagcgtgctc atcaccgcca tcaccgacag cggcgggttt
     1681 ttcatcttcc tcggtctcgc caccctgttc ctcgtctgaa accgccccaa tccaggggca
     1741 atccggccgc acaggccgtc aaatcagcca gttatccggg caacgttgca ggggcgcggg
     1801 cctgtgccat atttcttagt agagagagga atccagaaat gctgcccaac accatccagc
     1861 tcatcagtca ggcgatcaag gaaaagcgct gcatcgccat ccgttatcac gatcagcgcc
     1921 agattcgcgt ggtggaaccg cacgcggtgt acacggacga acgcggcgag ttggtgatgg
     1981 acggttacca gacacgcggc cattccgctt ccggacgccc gccgccgttc tggcggccgt
     2041 tccgcatgaa caagatcacc gccgtgtcgg tgctgaagga gaccttccag ccgcgcatcg
     2101 cggaagggtt cagcgccaac cgtctcaagt accgcagcgg cttggtggcg atcgtgcagg
     2161 acagccagcc ggctttcgcc tacgtgtatc cggcccagac cacggaaatc ggcccgcaac
     2221 tgccggaagg catgcgccgt tgatcgattc agggaattac tgctgcaaga caagccggct
     2281 caatgccggc tttgtttttc cacgcgtgcg cgcagctcac gcggatcacc cagcttcatt
     2341 aaatccttgg cgaagcgtgt gagctgaccg agattggctt cacgcacgat cttcttgacc
     2401 tccagcaggg tggcggggtg catgctgaac tcggagagac cgagccccag cagcagccgc
     2461 gtgtagcgcg gatcacccgc catttcgccg cacatcgcca ccggaatgcg cgccttcctg
     2521 ccggcctgaa tcgtgatgga aatcaggcgc agcaccgagg gatgcagcgg gtcgtaaaga
     2581 tagttcacgg catcatccac gcggtcgatg gcgagtgtgt attgaatgag gtcgttggtg
     2641 ccgatggaga agaaatcgag atgcggcgcg aacaggtccg ccgagaccgc cgccgccggc
     2701 acctcgatca tgccgccgat cggaattttc gggttgaact tgatgtgctc gcgcttgaga
     2761 tcgctcttgg tctcctctat caggtcgagc acctgaaaca gttcttcctg gccggacagc
     2821 atgggaatca tcctgcgcac tttgccgaag gccgaggcgc gcaggatggc gcgcaactgt
     2881 gggcgaaaaa gagacgtgtc gtgcaggca
//
LOCUS       DBNGZE010000091         4931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1609563, whole genome shotgun sequence.
ACCESSION   DBNGZE010000091 DBNGZE010000000
VERSION     DBNGZE010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1609563"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="AC16FV_02315"
     CDS             <1..1425
                     /locus_tag="AC16FV_02315"
                     /inference="COORDINATES: protein motif:HMM:NF037276.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HzsA-related protein"
                     /protein_id="HIR2848929.1"
                     /translation="ENRVARFFRITKAVPTPRGLSRETVGETEFEMQQIVGYGTIEPD
                     GSIRTRVPSDTPITITALDKEGRAFTPHTNWIQAREGERRFCKGCHSSRLSTTNSSDG
                     LALNDPVQVGIHPSGDATSTMARTRSAVLDPSALDLKRDLVSQDVWTPLYNSRDKTNL
                     AAQSTISITYSGLTTSAPSIKGPASCAVTWSAKDCAIVINYEDHVQPIWNAKCVSCHQ
                     GANPAAGLDLSNVRSGEFGRFLSYQELLVGDPLIDPATGLPQITIDADGDVRIAREAA
                     AVESGASRASRLIERLFEQPLKATALTGSLRAVCRAGGASCINGATYQSHASLLNVSE
                     RRLVTEWIDIGAQYYNDAFDNGGNVRSVAAQLSETVFTSCIQPILQQNCASCHQPFGG
                     TGASGGTVNTQFTANRFILTGNAAADFSVTAAMVNNLAVPDNSYLLTRPSNPPHPTGV
                     LSATNRNIIRAWIADTLNATTCSP"
     gene            1439..1954
                     /locus_tag="AC16FV_02320"
     CDS             1439..1954
                     /locus_tag="AC16FV_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848930.1"
                     /translation="MGGGVRMLWMGACLCALVSCGDQVPVAPATQDADLCLNDFQNCV
                     MPVLSGQIRRRGGAVVSCMDGNCHVAGGNGGRFTLGADVSANFEAVKSNFVDFASTHD
                     SLILVEPTQDDVSPSATAAFHGGGEIFPSRSDACYSAIHNWISNQVVNQSSAACGLCA
                     PIADTFASCGY"
     gene            1957..2745
                     /locus_tag="AC16FV_02325"
     CDS             1957..2745
                     /locus_tag="AC16FV_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR2848931.1"
                     /translation="MRITVARQGNERRRSLGRIKTAFLLCLLLFGAMAARADEEVSRV
                     LVADPYLELHTGPGRGYPVFYVAERGEWIEILKRRTDWFKVRTARDKEGWVTRSQMEN
                     TLTDAGTRKTFRDVLLDDYLRRRLEFGFSFGHFENDPVLTARAGYRLHDNLIAELAIS
                     QVTGDFSSTSLFYAAIVSQPFPEWRASPFFTIGLGRFKNTPKATLVSAVETDADLANA
                     GLGLHYYITRRFIFRADYKQHIALIGSDRTDSYKEWSMGISFFF"
     gene            2764..3402
                     /locus_tag="AC16FV_02330"
     CDS             2764..3402
                     /locus_tag="AC16FV_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HIR2848932.1"
                     /translation="MKNPITLLLLRQIALGVCLLLAGQAFAGQPEQVIQPELDRREIH
                     VPKIDAEDVEMGIYGGTLSVQDFGARSVTGARLAYHVTEDIFVEGVMARSTVSDESFY
                     RFGIPIFREREVDLTFYNLSVGVNLFPGEVFIGRNWAMTSAVYLIGGVGNVNFNEEDH
                     NAFNFGIGIRLLPIDWLAVRAEIRDHLFESDVLGKNEIKQNFELSLGLSVYF"
     gene            3422..3943
                     /locus_tag="AC16FV_02335"
     CDS             3422..3943
                     /locus_tag="AC16FV_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2848933.1"
                     /translation="MTRPARSIFAFVFICMTLGGMASARTAIAALPAVGVRAPDFALN
                     SSTGRNMRLSESRGEVVLINFWATWCGPCRQEMPQLGRLYEQYRVAGFTLLGVNIDDS
                     RANAEAMVKKLGVRFPTLFDSDKRVARLYDVDTMPATLLIDRDGRVRYVHRGYRDGYE
                     RKYQEQIRELLKE"
     gene            3940..4170
                     /locus_tag="AC16FV_02340"
     CDS             3940..4170
                     /locus_tag="AC16FV_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HIR2848934.1"
                     /translation="MMRRFVKIAGVVTLVAQIAGCTTGPWVKPYERESLADPIMSFSR
                     DPLSDMYLQHVYDVREAARGAGVSQGGGCGCN"
     gene            4157..>4931
                     /locus_tag="AC16FV_02345"
     CDS             4157..>4931
                     /locus_tag="AC16FV_02345"
                     /inference="COORDINATES: protein motif:HMM:NF023516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HIR2848935.1"
                     /translation="MDVIRRAVPTLLFAVSLTQAAVLPEDRADAMYHYYDGGGTKVDG
                     PALLVRKGVADTASVYASYYADSISGASIDVMTTASPYKETRQERGVGVDYLHRNTTM
                     NLSYTASEERDYLANTLGVNVAHEVFDGLSTITLGYTVGRDEVGKTGTAFKEDINRYQ
                     YRLGLSQVFTRTFLMNFEYEGILEDGYLNSPYRAARLQGLLVPERYPGARDSYAFAVR
                     ALKGLMSADDKLESSVQLGYRYFWDTWDIRASTLEIGYQR"
BASE COUNT          973 a         1465 c         1524 g          969 t
ORIGIN      
        1 gaaaaccggg tggcgcgttt cttccgcatc accaaggcgg tgccgacacc gcggggcctg
       61 tcgcgcgaga cggtcggcga aaccgaattc gagatgcagc aaattgtcgg ctacggtacg
      121 attgaacccg atggctcgat ccgcacccgt gtgccgtccg atacgccaat cacgatcacg
      181 gcgctcgaca aggaaggacg ggcgttcacg cctcatacca actggatcca ggcacgcgag
      241 ggcgagcgcc ggttctgcaa gggctgtcat tcctcgcgcc tcagcaccac caacagcagc
      301 gatggactcg cgctgaatga tccggtccag gtcggcatcc atccctcggg tgatgcgacc
      361 agcaccatgg cgcgcacgcg cagcgccgtg cttgatccgt ccgcgctcga cctgaagcgc
      421 gatctggtgt cgcaggacgt ttggacgccg ctttacaaca gccgcgacaa gacaaatctc
      481 gcggcgcaaa gcaccatctc catcacatac agcggactga cgacgtcggc gccttcgatc
      541 aaaggccccg cttcctgcgc cgtcacatgg tcggccaagg actgtgccat cgtgatcaat
      601 tatgaagatc acgtccaacc gatctggaat gccaagtgcg tcagttgcca ccagggcgcg
      661 aaccccgccg ccgggcttga tctcagcaac gtccgctcgg gcgaattcgg acgcttcctg
      721 tcgtatcagg agttgctcgt gggagatcca ttgatcgatc ccgccaccgg cctgccgcag
      781 atcacgatcg atgcggacgg cgacgtcagg atcgcgcgcg aagccgccgc cgtcgagagc
      841 ggtgcttccc gtgccagccg gctgattgaa cgtctgttcg aacagccgct caaggccacc
      901 gcgctcaccg gcagcctgcg tgccgtgtgc cgtgccggag gcgcgtcgtg tattaacggc
      961 gccacctatc agagccacgc cagcctgctg aacgtctcgg agcgacggct ggtcacggaa
     1021 tggatagata tcggggcaca gtactacaac gacgccttcg acaacggcgg caacgtgcgt
     1081 tcggtcgccg cccagctgag cgaaaccgtc tttaccagct gtatccagcc gatcctgcag
     1141 cagaattgcg cgagctgtca ccagccgttt ggcggtacag gcgcgagcgg aggcaccgtg
     1201 aatacccagt tcacggcgaa ccgcttcatt ctgaccggca acgccgcggc tgacttcagc
     1261 gtcaccgccg cgatggtgaa taacctcgcg gtgccggaca acagttacct gttgacgcgg
     1321 cccagcaacc cgccgcaccc caccggcgtg ctgagcgcga ccaatcgcaa catcattcgg
     1381 gcatggatag ccgatacact gaatgcgacg acgtgcagtc catgaggtcg agataacaat
     1441 ggggggcggc gtgaggatgc tgtggatggg cgcctgcctg tgcgcactgg tgtcctgcgg
     1501 cgatcaggtg ccggtcgcgc cggcgacgca ggacgcggat ctgtgcctga acgacttcca
     1561 gaactgcgtg atgccggtgt tgtcaggcca gatacgccgg cgcggaggcg ccgtcgtgag
     1621 ctgcatggac ggtaactgtc acgtggccgg cggcaacggc ggacgtttca cgctcggagc
     1681 ggatgtgtcc gccaacttcg aggccgtcaa aagcaacttt gtcgattttg ccagcacgca
     1741 cgacagcctg atcctggtcg agcccaccca ggacgatgta tcgccatcgg ccaccgcggc
     1801 gtttcatggc ggaggagaga tcttcccgag ccgcagcgac gcgtgttatt cggcgattca
     1861 taactggatc agcaatcagg tcgtcaacca atcgagcgct gcctgcggtt tgtgcgcccc
     1921 gatcgccgat accttcgcga gctgtggcta ttgaacgtgc gcattaccgt ggcgaggcag
     1981 gggaatgagc gccgacgttc cctcggccgg atcaagacag cgttcctgtt atgcctgctg
     2041 ttgttcgggg caatggccgc gcgcgccgac gaggaggtca gcagggtatt ggtcgcggac
     2101 ccctatctcg agcttcatac cggtccgggg cgcggctatc cggtgttcta cgtggccgag
     2161 cgcggcgaat ggattgagat actcaaacgg cgtactgact ggttcaaggt acgcacggca
     2221 cgggataaag aaggttgggt cacgcgcagc cagatggaaa acacacttac cgacgccggg
     2281 accaggaaga cttttcgcga tgtgctgctt gatgattatc tgcggcggcg gctggagttc
     2341 ggattctcct tcggccactt cgagaacgat cccgtgctga cggcgcgggc aggttaccgg
     2401 ctgcatgaca atcttatcgc tgaactggcg atttcgcagg ttacgggcga tttctccagt
     2461 acttcgctct tctatgcggc gatcgtgtct cagccgtttc ctgaatggcg tgcttcgcct
     2521 tttttcacta tcgggctggg acgcttcaaa aacaccccga aggcgacgct cgtgagcgcg
     2581 gtcgagacgg acgcggacct ggccaacgca ggcctgggac tgcactatta catcacgcgg
     2641 cgttttattt tccgggcaga ttataaacag catatcgcgc tcatcggctc cgatcgcacc
     2701 gattcgtaca aggagtggtc gatgggcatc tcatttttct tttagaccag gcggtcccaa
     2761 atggtgaaaa accccataac cctgttattg cttcgccaga ttgcgctcgg cgtttgcctg
     2821 ctgcttgcgg gacaggcatt tgccgggcaa ccggagcaag tgattcagcc ggagctcgac
     2881 cgccgcgaaa ttcatgtccc taaaatcgac gcggaagacg tcgaaatggg aatctacggc
     2941 ggtacgctga gtgtacagga cttcggcgcc cggtccgtta ccggtgcacg tcttgcttat
     3001 cacgtgaccg aggacatttt cgtcgaaggt gttatggcgc gttccaccgt ttcggatgaa
     3061 tcgttctatc gtttcggtat tcctattttc agggaacgcg aggtggacct gacgttttat
     3121 aacctgtcgg taggcgtcaa cctgtttcca ggagaggttt tcatcggcag gaactgggcc
     3181 atgacatcgg cggtgtatct catcggggga gtcggcaatg tgaacttcaa cgaggaagat
     3241 cacaacgcct tcaacttcgg catcggtatc cgcctcctgc ccatcgactg gttggcggtc
     3301 cgggccgaaa tacgcgacca ccttttcgaa tctgacgtct taggcaagaa cgagatcaag
     3361 cagaatttcg agctcagtct gggattgtcg gtgtatttct gatgaaacaa agaggccaac
     3421 catgacaaga ccggcacgat cgatcttcgc gtttgtgttc atctgcatga ccctcggcgg
     3481 catggcgtcc gcgcggacgg ccatcgcggc cctgcccgcg gttggcgtgc gcgccccgga
     3541 ttttgccctt aacagcagca cgggccgcaa catgcggctg agtgaatcgc gcggcgaagt
     3601 ggtgctgatc aacttctggg ctacttggtg cgggccgtgt cgccaggaaa tgccgcagct
     3661 cggccggttg tacgagcaat accgcgtggc ggggttcacg ctgctgggag tcaacatcga
     3721 tgacagccgc gccaacgccg aggccatggt gaaaaaactc ggcgtgcgtt tcccgaccct
     3781 attcgacagt gacaagcgcg tggcccggct gtatgacgtg gacacgatgc cggcgacgtt
     3841 gttgatcgac cgcgacggcc gggtgcgcta cgtgcatcgc gggtaccgcg acggttacga
     3901 gcggaaatac caggaacaga tccgggagtt gctcaaggaa tgatgcgccg ctttgtcaaa
     3961 atcgccggag tcgtgacact tgtcgcgcag attgcaggct gcaccaccgg gccgtgggtc
     4021 aagccgtacg aacgcgaatc gctcgccgat ccgatcatgt cattttcgcg cgacccgctc
     4081 tcggatatgt acttgcagca tgtttatgac gtgcgcgaag cggctcgcgg cgccggtgtc
     4141 agccagggcg gcggatgtgg atgtaattag acgggcagtg ccgacgttgt tgttcgccgt
     4201 gtccctgacg caggccgcgg tgcttcccga agaccgggcg gatgccatgt atcactatta
     4261 cgacggcggt ggaacgaagg tggacggtcc ggcgctgctg gtgcgcaaag gcgttgcgga
     4321 tacggcctcg gtgtacgcgt cgtactatgc cgacagcatc agcggcgcct cgatcgacgt
     4381 gatgacgacg gcgagtccgt acaaggaaac gcgccaggag cgcggcgtgg gagtcgacta
     4441 cctgcatcgc aatacgacca tgaacctgtc atacacggca agcgaggaac gcgactacct
     4501 tgcgaacacg ctgggcgtca atgtcgcgca tgaggtgttc gatggcctga gcaccattac
     4561 cctcggatac accgtgggcc gggacgaggt cggcaagacc ggcacggcct tcaaggaaga
     4621 tatcaaccgc tatcagtatc ggctgggatt gtcacaggtg tttaccagga cattcctcat
     4681 gaatttcgaa tatgagggta ttctcgaaga cggttatctc aacagcccct accgcgccgc
     4741 ccggctccag ggattgctgg tgccggaacg ttatccgggc gcacgcgaca gttacgcctt
     4801 cgccgtgcgc gccctgaaag gactgatgtc cgccgacgac aagctcgagt catccgtgca
     4861 acttggctat cgctatttct gggacacctg ggacattcgt gcctccaccc tcgagatcgg
     4921 ctatcagcga a
//
LOCUS       DBNGZE010000092         3935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1262110, whole genome shotgun sequence.
ACCESSION   DBNGZE010000092 DBNGZE010000000
VERSION     DBNGZE010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1262110"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..760
                     /locus_tag="AC16FV_02350"
     CDS             269..760
                     /locus_tag="AC16FV_02350"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR2848936.1"
                     /translation="MKKAVAGKPVPDAEMQTTGGKTLRLHDLKGKYTVLYFYPKDDTP
                     GCTLEGQDFRDRHDQLKKLKAGIYGISRDSLALHEKFKAKFKFPFELVSDPDEKLCRL
                     FDVIREKSLYGKKYMGVDRSTFILDKDGVLRKEFRGVKVKGHVDEVIEEIRKLQTPSA
                     KRK"
     gene            757..2157
                     /locus_tag="AC16FV_02355"
     CDS             757..2157
                     /locus_tag="AC16FV_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR2848937.1"
                     /translation="MTAKRKIFVVDTNVLIHDPTSILRFQEHDVVIPIVVLEELDNIK
                     VGMSEIARNVRQVSRLLDELVEKANGDISHGIKLPSVSKDIETGHLYFHMEEARSPLP
                     FGLSGRSSDNALLGITLDLSKSHPDRQTILVSKDINLRLKARALGIRAEDYTNDQVLD
                     DANLLFTGSEKLAADFWETHSKNMESWKEEGRTFYRLRGPKARVWLPNQFLYINGSNT
                     EKGFEAVVRRLENDSVIIEVVKDYASERNKVWGISARNREQNFAFNLLMDPEVDFVSL
                     LGQAGTGKTLLTLASALMQTLESKRYTEIIMTRMTVPVGEDIGFLPGTEEEKMGPWMG
                     ALEDNLDVLQETATQEHGAWARAATHDLLRNRIRIKSLNFMRGRTFLKKFLIIDEAQN
                     LSPHQIKTLVTRAGPGSKIVCLGNIAQIDTPYLTETTSGLTYVVDRFKQWEHSGHITL
                     QRGERSRLADYASEIL"
     gene            complement(2177..2563)
                     /locus_tag="AC16FV_02360"
     CDS             complement(2177..2563)
                     /locus_tag="AC16FV_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2848938.1"
                     /translation="MTPVAGATEKRLNEILQRLPAAQAEALLEFAEFLLVRHGAVVEE
                     GKPSGAVEVPAPVDIPRPAEETVVKAVKRLRATYPMLDARKLLNQTSELMTQHIVQGR
                     ETIEVIEELEILFRSHYEKLVVNNKD"
     gene            complement(2560..3477)
                     /locus_tag="AC16FV_02365"
     CDS             complement(2560..3477)
                     /locus_tag="AC16FV_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HIR2848939.1"
                     /translation="MADRRLQVFHTVARLLSFTKAAETLHMTQPAVTFQIRQLEEYFN
                     TRLFDRTHNRISLTAAGERVFEYAERIIALYSEMDNRVRELTGDVSGILIIGASTTIA
                     EYMLPSLLGEFKEKHPGINIRLKVSNSVGIVHMVEDNSVDVGIVESPIANKNLVVEVC
                     WHDKLVFICPPQHPLAKKPKITVEDFQGLPFVVREEGSGTREFINEYLAKNNMQLHDL
                     NVNVEVGNPEAVKSAVEAGLGVSIVSQATVVKELKLGTLAARPLEPQLERPFSIVFQR
                     QKFRLRAMDEFMDFAHEHCEKRSANNSPK"
BASE COUNT          869 a         1177 c         1119 g          770 t
ORIGIN      
        1 cggagaaggt tgagcaggcg atgaatgaga gaaagaaaca ttggaaaatg ccgaagttcc
       61 ctcgacgatc gggggtctta aaacgatatc tccaatccgc tgccccagct tataaagggg
      121 cttatacgga atattgatct tttcaccccc accctgaccc tcccccgtca agggggaggg
      181 aatttaattt tgagatactt ctgcaagccg tccctgttct ccgaggtcaa cgacctatac
      241 aattcaccga caggagttgg aacacaccat gaaaaaagca gttgcaggga agcccgttcc
      301 cgacgcggaa atgcagacca ccggcggcaa gaccctccgc ctgcatgacc tcaagggcaa
      361 atacaccgtg ctgtatttct accccaagga cgacacccct ggctgcacct tggaaggcca
      421 ggatttccgc gacagacacg accagctcaa gaagctcaag gccgggatct acggcatatc
      481 gcgcgacagt ctcgccttgc acgaaaaatt caaggccaag ttcaaattcc ccttcgaact
      541 cgtctccgac ccggacgaga aactctgccg actgttcgac gtcatccgtg aaaaaagcct
      601 ttatggaaaa aaatacatgg gcgtggaccg cagcaccttt atactcgaca aggacggcgt
      661 gttgcgcaag gaattccgcg gcgtcaaggt aaaggggcac gtcgacgaag tcatcgagga
      721 aatccggaaa ctgcaaaccc catcagcaaa gcgtaaatga ccgccaaaag aaagattttc
      781 gttgttgaca ccaacgtcct cattcacgat ccgacttcga ttttgcgttt tcaggagcac
      841 gatgtggtca ttcccatcgt ggtgctggaa gagctcgaca acatcaaggt cggtatgtcc
      901 gaaatcgcgc gcaacgtgcg ccaggtcagc cgcctgctgg acgaactggt ggaaaaggcc
      961 aatggcgaca tcagccatgg catcaaactg ccatctgttt ccaaggacat cgaaaccggg
     1021 catctctact ttcacatgga agaagcacgc agcccgctgc cgttcggcct ctccggccgc
     1081 agctcggaca atgccctgct cggcatcacg ctcgacctga gcaaaagtca tcccgatcgt
     1141 cagacaattc tggtttcgaa ggacatcaac ctgcgcctca aggcgcgcgc gcttggtatc
     1201 cgtgccgagg attacaccaa cgaccaggtt ctggatgacg ccaacctgct tttcaccggt
     1261 tccgagaaac tggccgcgga cttttgggaa acgcacagca aaaatatgga gtcatggaag
     1321 gaagaaggcc gcacgttcta ccgcctgcgc ggacccaagg cgcgcgtctg gttgcccaac
     1381 cagtttctgt acatcaacgg atcgaacacc gaaaaaggct tcgaggcagt ggtgcgccgc
     1441 cttgagaacg attccgtcat catcgaggtc gtcaaggatt atgcgtcgga gcgcaacaag
     1501 gtctggggca tctcggcgcg taaccgcgag cagaatttcg ccttcaacct gctcatggat
     1561 ccggaggtcg atttcgtcag tctgctcggt caggccggca ccggcaagac cctgctgacg
     1621 ctcgcctcgg ctctgatgca gacgctggaa tccaagcgtt acacggaaat catcatgacg
     1681 cgcatgacgg taccggtcgg cgaggacatc ggctttctgc ccggcaccga agaggaaaag
     1741 atgggcccct ggatgggcgc gctcgaggac aatctcgacg tattgcagga aaccgccacg
     1801 caggaacacg gcgcctgggc gcgcgccgcc acgcacgacc tgctgcgcaa ccgcatccgc
     1861 atcaagtcgc tcaatttcat gcgcgggcgc acgttcctga agaaattcct gatcatcgac
     1921 gaggcgcaga acctgagtcc acaccagatc aaaactctcg tcacccgcgc cggccccggc
     1981 agcaagatcg tctgcctcgg caatatcgcc cagatcgaca cgccctacct gacggaaacg
     2041 acttccggcc tgacctacgt cgtggatcgt ttcaaacagt gggaacacag cggacacatc
     2101 acattgcagc gcggcgaacg ctcgcgcttg gcggattacg cgtcggaaat cctctgaaaa
     2161 aaactacaaa agagaatcaa tctttgttgt ttacaaccag tttttcgtag tgcgatcgaa
     2221 acaggatctc cagctcctcg atcacctcga tcgtctccct gccctgcacg atatgctgcg
     2281 tcatcagctc tgaggtctgg ttgagcagtt tccgcgcatc cagcatgggg taagtcgcgc
     2341 gcaaacgttt gacggccttg acgacggttt cttcggcggg gcggggaata tcgaccggag
     2401 cgggaacctc gacggcaccg gacggctttc cttcctctac aacagctcca tgacgcacaa
     2461 gcaggaactc ggcgaactcc agcaaggcct cggcctgcgc ggcgggaagc ctttgcagaa
     2521 tctcgttcag gcgtttttca gttgcgccgg ccacgggcgt catttcgggg agttgttggc
     2581 ggatcgcttt tcgcagtgtt catgcgcgaa atccatgaat tcatccatgg cgcgcaaacg
     2641 gaatttctgg cgctggaaaa cgatggagaa aggacgttcg agctgaggct cgagcgggcg
     2701 cgccgccagc gtgccgagct tcagttcctt caccaccgtc gcctgtgaga cgatggaaac
     2761 tcccaagcca gcctcgaccg cgctcttgac tgcctcgggg ttgcccacct caacattgac
     2821 gttgaggtcg tgcaactgca tgttgttctt ggcgagatat tcattgatga actcgcgcgt
     2881 gccggagccc tcctcgcgca ccacgaacgg cagcccctgg aagtcctcga ccgtgatctt
     2941 gggtttcttc gccaacggat gttgcggcgg gcagatgaac acgagcttgt cgtgccagca
     3001 aacctcgacc acgagattct tgttcgcgat cggcgactcg acaatgccga cgtccaccga
     3061 attgtcctcg accatgtgca cgatgcccac ggaattcgac accttgaggc ggatgttgat
     3121 gccgggatgc ttttccttga attcaccgag caggctgggg agcatgtatt ccgcgatggt
     3181 ggtgctggcg ccgatgatca ggatgccgct gacgtcgccc gtcagttcgc gcactcggtt
     3241 gtccatctcg gaatagagcg cgatgatccg ttccgcgtat tcgaaaacgc gctcgccggc
     3301 ggccgtgagg ctgatgcggt tgtgggtgcg gtcgaacagc cgcgtgttga aatactcctc
     3361 cagctgccgg atctggaacg tcaccgccgg ctgcgtcatg tgcagcgttt ccgctgcctt
     3421 ggtgaagctc agcagccgcg ccacggtgtg aaaaacctgc agtcttcgat ccgccatcga
     3481 attatctcgt acgccccggg aggccgttat tcatcttgga aacgtggatt atgtaggaca
     3541 gcgcgcttaa ctcaaagccg gtccggctag cagtggattt atcccgcgag ggcgtgccgg
     3601 ttggcgagtc tagaagctat ctcaaaaacg tcgcgagcgg ccgtcagccg ggtgagggta
     3661 aggcggggat ttgctcgccc catcccctgg ggctcgcccc ttcgcgcttc gcgctccggg
     3721 gccggcgtcg cttggctccg ccgtccaatt ctgctcccag cagaattgtc acgaacgtcc
     3781 ggtgggcgag cggcgggctt aggagccgcg agcgccaagc caaggatggc ttggctggct
     3841 ttctgcgcga gcagggacgc gaagcgcaga aattcgggcc accgaacggg ccgccaggga
     3901 cggcggcccc ggactttccc gcgcagccgg atggc
//
LOCUS       DBNGZE010000093         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_776631, whole genome shotgun sequence.
ACCESSION   DBNGZE010000093 DBNGZE010000000
VERSION     DBNGZE010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_776631"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16FV_02370"
     CDS             <1..380
                     /locus_tag="AC16FV_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848940.1"
                     /translation="RTRSAGKTSALRFPASREVQPGHPWPGVRGAYSRRAPTGLFAIP
                     RLTPKEVGDVYTADPIQLIDFPYMSVELTHSAGLNFLLETTPCLVSARECANVPGITA
                     VFGPITLLMFGGARFSEFIGPNF"
     gene            578..1567
                     /locus_tag="AC16FV_02375"
     CDS             578..1567
                     /locus_tag="AC16FV_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HIR2848941.1"
                     /translation="MKSSFKKTAIASVIAGTFAAAAIPAAEANVLNVSWRGAFTMLNP
                     AGAPVANSPSDYAAGYWANGNGVSGVGGAPYYPGNVTPGSDCTTFAPACVTTPAWYGN
                     RTPVSGTMSFDTSTGAGVATVNDFFFFGDTPGSGSGTSVARALGVTFQVIDTVGTMVG
                     AMLFSWNGLGHSVSIVLDASGMFGSLPAGPTTTISGVGALPATNGMDFDATKNVKTMP
                     LGPAPAATKTTNTGAGCDGLTLATQVNAYTINTNMANLGTCTTGMSDDGIGADPMASA
                     AFSGFNANFDILSVHVDSFVPTPVSAVPVPAAVWLFGSGLLGLVGIARRKKKA"
     gene            1947..2753
                     /locus_tag="AC16FV_02380"
     CDS             1947..2753
                     /locus_tag="AC16FV_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848942.1"
                     /translation="MPMTSLAMVAKRIAAGAAFAFCVVCLPAQAASVTVDGNLSDLES
                     IPWYIGWNDTSAVNVYRGIDPLGSTDSPTSESNNGFDISNVYAYYNWNDDTFYFGMKF
                     YGTVGDSQPLGSTATNEGIANTGTGSNVRAQFDSNETYGIQLYLGTSTADPQLLLYNV
                     KGANDSTDTVAAIYNPYNLTITHGVSESFNGVEFGVTGLRLTGAAPEPHPNMFTIRFS
                     AGSADTNSTSTAAEDFHLVTMSAIPVPAAVWLFGSGLAGLLAVARKRRRK"
     gene            2881..>3400
                     /locus_tag="AC16FV_02385"
     CDS             2881..>3400
                     /locus_tag="AC16FV_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848943.1"
                     /translation="MANAATNTYTMTDPQEITAFNSGYDVSRVVFTLIDSETLQIDVI
                     PYGVPGDADGDGNPNASSNPTIADGPGVSGLETIYMGMICNYTGGPCEPNLNITYADN
                     VLSATTSSGNDVSAHLSFQILDAIDNDGKLDTYRLTINNLSTVRTAVLGYVPTSVTFG
                     SFTFSAHFDDAQP"
BASE COUNT          778 a          948 c          901 g          773 t
ORIGIN      
        1 tgcgcacccg ttcggcgggc aaaacttcag cgctgcgctt tcctgcttcg cgcgaagtcc
       61 agcccggcca tccctggccg ggcgttcgcg gcgcgtattc tcgccgcgca cccaccggac
      121 tttttgcaat accccgcctc accccgaaag aggtgggaga tgtttatacg gctgatccca
      181 ttcaattgat tgattttcca tatatgtcgg tggaattgac acattcagcc ggcctgaatt
      241 tcttgcttga aacgactccg tgcttagtct cggctcgtga atgtgcaaat gttccgggaa
      301 taacagcagt ttttgggcct ataactttgc tcatgtttgg cggagcacgg ttttcagaat
      361 ttatcggtcc gaatttttag gtattcatca tgatttattt gtcggaataa tttacaacct
      421 gaaaaatatc gactccattt ttttatataa ctaattgttt atataaataa taatcattgt
      481 ggcaaggaat ttgcttttgt gaccgacagc agagaccgcg attaggcggc tgcaaggttg
      541 tttcatacaa atcaatcaag atttaaggag gagcttcatg aagtcttctt tcaagaaaac
      601 agcaatagcc tccgttattg ccggaacgtt cgctgcagcg gcgatcccgg cggcggaagc
      661 gaacgtgctt aacgtctctt ggcgtggcgc gttcaccatg ctcaacccgg ccggcgcgcc
      721 ggtggcaaac tcgccgtcgg actacgccgc gggctactgg gccaacggta atggtgtttc
      781 tggcgttggt ggtgccccgt actatccggg caatgtaacc ccgggttctg actgcacgac
      841 ctttgccccc gcctgcgtga cgacgcctgc ttggtacggt aaccgcacac cggtgtccgg
      901 caccatgagc ttcgacacct cgaccggtgc cggtgtcgcg acggtgaacg acttcttctt
      961 cttcggcgat actccgggat ccggttcggg caccagcgtg gcccgtgcgc tcggcgtgac
     1021 cttccaggtc atcgatacgg ttggcaccat ggtcggcgcc atgctgttct cctggaacgg
     1081 cttgggtcac tccgtgtcca tcgtgctcga tgcctccggc atgttcggca gcctgcccgc
     1141 gggaccgacc acgaccatct ccggcgttgg cgccctgccg gcgacgaatg gcatggactt
     1201 cgatgcgacc aaaaacgtta agaccatgcc actcggacca gctccggccg ccaccaagac
     1261 gaccaacact ggcgcgggtt gcgacggctt gacgctggcg acgcaggtga acgcctacac
     1321 catcaacacc aacatggcca acctcggtac ctgtaccacg ggcatgtcgg atgacggtat
     1381 cggcgccgac ccgatggcga gtgccgcgtt ctcgggcttc aacgcgaact tcgacatctt
     1441 gtcggtccac gtcgacagct tcgtgccgac accggtatca gccgttcccg tgccagccgc
     1501 agtctggctg ttcggctcgg gtctgttggg tctggttggt attgcccggc gtaagaaaaa
     1561 ggcctaacgc agaaattgtc ctgacaggtc ataacagcaa acaaactgtc ggaacaagta
     1621 ctgcaaatgg gcttttggaa taaaaataat cggtaaaagt gaagggtgcg gcccgaaagg
     1681 accgcaccct gacacttgtc cgaaacgtat tcggagttcg gaggagaaat taataacaag
     1741 taattttggg gaaactttta aatgacaggg ggtatgcaca tactctctgc cgttttttgt
     1801 tcagtcttgg gacgttagta ggtttatcca agaatgaaga aaaaaaggta aaaataaaaa
     1861 acccccagcc aggaaaacct ggagaatgca ggaagcactg gcgcatataa cccgaaagac
     1921 atgtttgcca caggagaaga ggggctatgc cgatgacaag cttggctatg gttgcaaaac
     1981 gcatagctgc tggcgcggcc tttgcgtttt gtgtggtttg cctccccgct caggcagcca
     2041 gcgtaactgt ggatggaaat ttatctgatc tggagtcgat tccctggtat atcggttgga
     2101 acgatacatc tgccgtaaac gtttatcggg gcatcgatcc tctgggcagc actgactcgc
     2161 cgacgtctga gtccaataac ggcttcgata tcagcaatgt ctatgcctac tacaattgga
     2221 acgacgatac gttctatttc ggcatgaaat tttacgggac cgtgggggat tcccaaccgt
     2281 tgggttcaac tgcgaccaat gaaggaatag cgaataccgg cactggcagc aacgtcagag
     2341 cgcaattcga ctccaatgaa acctacggca tacagcttta tctcggcacc tctacagcag
     2401 acccccagct tctgctgtat aacgtcaagg gcgctaatga cagtacggat accgttgccg
     2461 ccatatacaa cccgtacaac cttacgatca ctcatggggt aagcgaatca ttcaatggcg
     2521 ttgaatttgg cgtcacggga ttgcgactga caggcgcagc acccgaacct catccgaaca
     2581 tgtttaccat tcgcttcagc gcgggatcgg cagacacaaa ctccacctca acggcagcag
     2641 aagatttcca tctggtgacc atgtcggcta ttcccgtgcc agccgcagtg tggctgttcg
     2701 gcagcggatt agcggggctt ctcgccgtgg cgcgtaagcg ccgcaggaaa tagcataata
     2761 aatatacgaa ataacgaaca caggtgattt cggagagcgt cactaatgaa taacgtatta
     2821 ccgaagaaga ggtatttccc ccggataatt cttgccgcga gttgtggcct cgctgttgcg
     2881 atggcaaacg ccgctaccaa cacctacacg atgaccgacc cgcaggagat caccgccttc
     2941 aacagcggtt atgacgtttc ccgcgtggta ttcaccttga tcgacagcga gacgttgcag
     3001 atcgacgtga ttccctacgg cgtgccgggc gacgcggacg gtgacggcaa tcccaacgcc
     3061 agctcgaatc ccacgatcgc cgatggcccg ggggtcagcg gtctggaaac catctacatg
     3121 ggcatgatct gtaactacac gggcggtccg tgcgagccca atctgaacat cacctatgcc
     3181 gacaatgtgc tttctgccac gacgagctcg ggcaacgatg tatccgcgca cctgtcattt
     3241 caaatccttg atgccatcga caatgacggc aagctggata cctaccggct gaccatcaac
     3301 aacctgtcca ccgtcagaac cgcggtgctg ggctatgttc ccacgtcggt cactttcggc
     3361 tccttcacct tctcggctca tttcgacgat gcccagcctg
//
LOCUS       DBNGZE010000094         2357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1818292, whole genome shotgun sequence.
ACCESSION   DBNGZE010000094 DBNGZE010000000
VERSION     DBNGZE010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1818292"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC16FV_02390"
     CDS             complement(<1..187)
                     /locus_tag="AC16FV_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848944.1"
                     /translation="MRTLKLLLIFSVMFSTVPAHAADKVTPENSEVQKKESPPERAER
                     PQREAVKMSPSVRQSAPR"
     gene            complement(354..887)
                     /gene="hslV"
                     /locus_tag="AC16FV_02395"
     CDS             complement(354..887)
                     /gene="hslV"
                     /locus_tag="AC16FV_02395"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR2848945.1"
                     /translation="MLHGTTILSVRRNGHVVIGGDGQVTMGNTVMKANARKVRRLYKD
                     QVIAGFAGGTADAFTLFERFEGKLEKHQGHLTRAAVELAKDWRTDRILRRLEALLAVA
                     DKTASLVITGQGDVIEPEGGLIAIGSGGPYAQAAARALMENTELNARVIVEQALNIAA
                     DICIYTNRSLTIEELTS"
     gene            complement(968..1852)
                     /locus_tag="AC16FV_02400"
     CDS             complement(968..1852)
                     /locus_tag="AC16FV_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR2848946.1"
                     /translation="MDIDLLKTFLEVYRTRHFGRTAENLYLTQSAVSARIRLLEETLG
                     APLFTRTRNDIQLTPAGTRMLKYAEAILNAWNRARQDAALGEEDKVSLAIGASYSLWD
                     ILLQDWMHRLYDAIPRVALQAEAHGPELLIRKLLDRALDVAFMFEPPQMAELEAREVA
                     SIKLIMVADRPQLTAREAVGNGYIMADWGTSFAIAHARHFPDMPPPAVRLGLGRMALA
                     FLLHNGGATYLAEQTAGEHLASGRLHRVDDAPIIDRQVYVVHPLASERKPLLEQALGL
                     FAPRQPVNTSVRTTAPAL"
     gene            complement(1975..>2357)
                     /locus_tag="AC16FV_02405"
     CDS             complement(1975..>2357)
                     /locus_tag="AC16FV_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR2848947.1"
                     /translation="FAFFYVCLHFLSWAWLDQYFVLADIVKDVAKRPYITVGFASFLM
                     LIPLAATSTNAMIRRLGAKRWQRLHRLVYLIGIGGVVHFLWLVKSDIREPLLYGAILA
                     LLLGFRLWDRTRRAPGVVASGAVR"
BASE COUNT          468 a          709 c          714 g          466 t
ORIGIN      
        1 gacgcggcgc cgactggcgc acgctcggcg acattttcac tgcctcccgc tgtggccgtt
       61 cggcgcgttc gggcgggctt tcttttttct gcacttcaga attttctggt gtcaccttgt
      121 cggcggcgtg tgcggggacg gtactgaaca ttacggagaa aattagaagg agcttcaatg
      181 tgcgcataga ccgtctccca taaagcaata aacagaatta acaggatttc tcaggattta
      241 caggattaaa gattttagag atttagaaaa tcctgttaat cgtgcttttt aatcctgtga
      301 atcctgtcca attttgtttt tcttggcggt caagttcatt ttgcaatatc ttttcacgaa
      361 gttaattctt caatagtcaa gctgcgattg gtatagatgc agatgtcagc ggcgatattc
      421 aaggcctgct cgacgataac gcgcgcattg agttccgtgt tttccatcag ggcgcgcgcc
      481 gccgcctgcg cgtagggtcc gccggaaccg atggcgatga gcccgccttc gggttcgatc
      541 acgtcgccct ggccggtgat gaccagcgag gcggtcttgt ccgctactgc cagcagcgcc
      601 tccaagcggc gcaaaatacg atcggtgcgc cagtccttgg ccagttccac ggcggcgcgc
      661 gtcaggtgtc cctgatgctt ttccagcttg ccctcgaaac gttcaaacag cgtgaaggca
      721 tcggccgtgc cgccggcaaa accggcgatc acctgatcct tgtacaggcg gcgcaccttg
      781 cgcgcgttcg ccttcatgac ggtgttgccc atcgtcacct gtccgtctcc gccgatgacc
      841 acgtggccgt tgcggcgtac cgataagatc gtcgtcccat gaagcagcgg ttgcaagaca
      901 ggctcctttc aataaagcgc gggatggttg tcgtaaaaat ccggcggatg attatgcggc
      961 ggccggctta aagcgccggc gcggtcgtgc gaacggatgt attgaccggc tggcggggcg
     1021 cgaacaggcc cagcgcctgt tccagcagcg gcttgcgctc gctcgcaagc gggtgaacga
     1081 cataaacctg acgatcgatg atgggggcat cgtccacgcg gtgcagacgc cccgaggcga
     1141 gatgctcgcc ggccgtctgt tccgccagat acgtggcgcc gccgttgtgc agcagaaacg
     1201 ccagcgccat gcgcccgagc cccaggcgca ccgcgggcgg cggcatgtcg ggaaaatggc
     1261 gcgcgtgcgc gatggcgaac gacgtgcccc agtcggccat gatgtagccg ttgcccacgg
     1321 cctcgcgcgc cgtcagctgc ggacggtccg ccaccatgat caatttgatc gaagccactt
     1381 cccgcgcctc gagttccgcc atctgcggcg gctcgaacat gaaggccacg tcgagcgcgc
     1441 ggtcgagcag cttgcgaatg agcaattccg gtccatgcgc ctcggcctgc agggccacgc
     1501 gcgggatcgc gtcatacagc cggtgcatcc agtcctgcag cagaatgtcc cacagactgt
     1561 aggaggcgcc gatggcgagc gaaaccttgt cttcttcgcc aagcgccgcg tcctgacgcg
     1621 cgcggttcca cgcgttcagg atggcctcgg cgtatttcag catgcgcgtg ccggccggcg
     1681 tcagctggat gtcattgcgc gtgcgggtaa acagcggcgc gccgagcgtc tcttcgagca
     1741 ggcgaatgcg cgcgctcacc gccgattggg tcaggtagag gttttccgcg gtccggccga
     1801 aatgtcgggt gcgatagacc tcgagaaagg tcttgagaag gtcgatatcc atctcttttc
     1861 agaaattctt ttatcagggc aaatcatttc actttgccct gaggctggca agcctgtccg
     1921 cgcctggtcg cgcggctcaa ctgtctattt ctgaaagccc atgcccggaa ggaatcaacg
     1981 gacggcgccg gaggccacca cgcccggcgc ccggcgcgtc ctgtcccaca accggaaccc
     2041 cagcaacaag gccaggatgg caccgtacag caacggctcg cggatatcgg atttcaccag
     2101 ccacaggaaa tgcaccacgc caccgatgcc gatgagatac accaagcggt gcagtcgttg
     2161 ccagcgcttc gcgcccagac gccggatcat ggcattggtg gaagtcgcag ccagcggaat
     2221 cagcatgaga aagctggcaa aacccaccgt gatatacggc cgcttggcca cgtccttgac
     2281 gatgtccgcg agcacgaaat actggtcgag ccaggcccag gacaggaaat ggaggcagac
     2341 gtagaaaaag gcgaaca
//
LOCUS       DBNGZE010000095         9257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_985416, whole genome shotgun sequence.
ACCESSION   DBNGZE010000095 DBNGZE010000000
VERSION     DBNGZE010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_985416"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="AC16FV_02410"
     CDS             <1..638
                     /locus_tag="AC16FV_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2848948.1"
                     /translation="LQAQLARWVGEAAYQPLPAELPPLPAVPETFDPTRHPDWLAAQY
                     GLEQARLEVDMARQEYKPGWLLDLSYGFRRPMPNGMERPDMVTAMVTLDMPIFRDKRQ
                     DRRLAEKQAMESGMRYEIEDKRRELEVMYRSMRAEYDALVEREKIFAQELLPATRRES
                     QVTVAGFARDQAEYREALMKTLDTELEYQRLRADRFKAQAELLYLTGEPQT"
     gene            635..1528
                     /locus_tag="AC16FV_02415"
     CDS             635..1528
                     /locus_tag="AC16FV_02415"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR2848949.1"
                     /translation="MNRKMKLTAIIGAVLVLGVAAGWYFLYSDKGPAGAGGDTKAERQ
                     VLYWTDPMVPGYRSDKPGKSPYMDMELVPVYADGTGDGGAPVVTIRPEFIQNFGVRTH
                     KVTLGGAARTLEAHGYLFRESGRLLVLVDTFERDTWMRAGLPAEIRVADFPGRQWSGT
                     VESVKSDVDIGTRSLKARIRITNPDAALSPNMYADVIISDTAQRNGKIMVPREALIRT
                     GARTAVVLALGSGRFQPVEVVPGEESGDWVEIRQGIKQGDTVVTSGQFLIDSEASVRA
                     SFQRMESAPVDPHAGHKEKKP"
     gene            1525..4662
                     /locus_tag="AC16FV_02420"
     CDS             1525..4662
                     /locus_tag="AC16FV_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2848950.1"
                     /translation="MIAHIIRWSIRNRFLVLLLTALVVAWGIFAVSRTPLDAIPDLSD
                     VQVIIQTSYPGQAPQVVEDQVTYPLTTAMLSVPGATTVRGFSQFGESFVYVLFKDGTD
                     LYWARSRVLEYLSQVLPRLPRGARPALGPDATGVGWVYEYALVDRSGKHDLAQLRTLQ
                     DWFLKYELRTVPGVAEVATVGGMVKQYQVVVDPVRLRAFRLTLMDLQRAIEKANQETG
                     GSVVEMAEAEYLVRVRGYLQTKSELRAVPLGVTSNGTPIRLENVADIRLGPEMRRGIA
                     DLNGEGEVAGGIIVMRHGENALATINAVKARIATLKNSLPEGVEIIPTYDRSEVIKNA
                     VHNLTGTLVEEFIVVAIVCMVFLFHLRSSLVVIVTLPIGILVSFIIMKYQGINANIMS
                     LGGIAIAIGAMVDAAIVMIENVHKRMEKQEVTDENRWAIVRSAAEELGPPLFYSLLII
                     ALSFIPVFALEAQEGRLFSPLAFTKTYAMAAAAALSITLVPVLMGYFIRGHIRGEQEN
                     PLNRGLIRAYRPMLDWVLARPKQVLWGALALVVLGAIPIMQLGSEFMPDMDEGDILYM
                     PTTLPSLASGKAQQLLQQTNRILKTFPEVESVFGKSGRAETATDPAPLSMFETTIQLK
                     PRSEWRPGMTTAKLIKEMNAATRFPGLSNAFVMPIKTRIDMLTTGIRTPVGVKVSGPS
                     LATLDSIGREIETALHDVPGTVSVYSERVTAARYVDVRIDRHRAARFGLNIADIQETV
                     ATAVGGMNITDTIEGRERYPVNIRYPQEIRDSLARLQALPLVTMSGLPIALSEVADVR
                     VVDGPDMIRSENARLSGWTYVDIRDRDIGSYVADAQRAVMEKVKLPPGYSLHWTGQFE
                     YMQRAVERLEVVIPLTLVIILLLLYLNFRNGMEVLIVIGTLPLALVGGFWLLYFLGYN
                     LSVAVAVGFIALGGVAVEIGVVMLAYLDQALKRRETERTAKDQRLTTDDVREAVIEGA
                     LLRVRPVTMTKVAIIAALLPILWSHGTGSEAMQRIAAPMVGGMLSVAILTLAVIPAAY
                     FLWQAWRVGKSVKKN"
     gene            complement(4659..4898)
                     /locus_tag="AC16FV_02425"
     CDS             complement(4659..4898)
                     /locus_tag="AC16FV_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR2848951.1"
                     /translation="MSQNEPDRTVDTSGECCPGPLREAHRAIKAMQPGEVLELISTDI
                     GSRMDIPAWCKRTGHELLRVEDDGKIFRYYVRKRS"
     gene            4974..5489
                     /gene="greB"
                     /locus_tag="AC16FV_02430"
     CDS             4974..5489
                     /gene="greB"
                     /locus_tag="AC16FV_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR2848952.1"
                     /translation="MGRNRLPPAKSSAYITPQGAQRLREELDHLWRVKRPQVTQAVSE
                     AAAQGDRSENAEYIYGKKQLREIDRRIRFLQKRLDVLKIVDRPPTDTSRVFFGAWVRL
                     ESDTGDIAEYRIVGPDESDINKGLISIDSPLARALLKKAADDEVTVSLPRGQTVYIVL
                     EVRYTPFDNTG"
     gene            complement(5515..7581)
                     /locus_tag="AC16FV_02435"
     CDS             complement(5515..7581)
                     /locus_tag="AC16FV_02435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2848953.1"
                     /translation="MFRLTRTYSIASFLGIVLVAVALGVFYRSIAVRSLIDQQTQANV
                     FLTQSLSNSLWPKYVDFIVHAAGIPVRELASQPEVASLREDVLQKIRGLHVVKVKIYN
                     MSGLTVYSTEAKQIGENKAGNSGFQKAKAGHTDSDLVFRDRFSATEQVIENRNLLSSY
                     TPVRRTPGGPVEGVFEIYTDVTPLVADIERTQYTVFGGVIFLLLLLYLFLLMIVRRAD
                     RIIRTHENAERRAQAEQMHYMAYHDALTGLPNRALFKDRFQHAVTVANRAQKSLGVMF
                     IDVDRFKVINDSLGHEGGDAVLIETAKRIRTCLRASDTACRIGGDEFTVILERLPSNE
                     HAAQVANRLIQKFAEPMKVGNREIIVTASIGIAIYPGATQDIQRLLKDADAAMHAAKE
                     TGRNCYVFYTQEMEARAQESFEYELGLRQALQNREFLIYYQPRVNTATGTVTGAEALL
                     RWQHPSRGLVMPDNFIPLLEDTGLVIPVGEWVLQQACLQGRRWQDAGHKSLNVSVNLS
                     MKQFRAGTLLNSVRQALDESGLPPRSLELEITETVLVDDAEQALDLMHQLKKIGVTLS
                     IDDFGTGYSSLNYLRRFPIDLLKIDGSFIRDVTRNRGDAAITTTIAVMAKSLHLGILA
                     EGVETREQARFLKTIGCHDMQGFLFGEPLPVENFGSRLGEMDVLKLAGFDQASGSDGA
                     GRPAMA"
     gene            complement(7622..7819)
                     /locus_tag="AC16FV_02440"
     CDS             complement(7622..7819)
                     /locus_tag="AC16FV_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848954.1"
                     /translation="METRTGKQRKARRLKVLAGGRKKAGSVDILSQADRDIVCHYGVA
                     VQCTSIEGMELVEFEFLPDAA"
     gene            complement(8058..8777)
                     /locus_tag="AC16FV_02445"
     CDS             complement(8058..8777)
                     /locus_tag="AC16FV_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2848955.1"
                     /translation="MTEPLLTVRDLEVSYGAVQVLFGVNIEVHAGEIVSIIGPNGAGK
                     STVIKAVIGLEPVQKGSVVFDGRPITNSPAHVIPALGITYVPQGRIVFAQMTVSDNLE
                     MGAFLQSDSRKRREAVQAVFDQFPRLYERRHQPAGKLSGGEQQMLAIGRALMMNPRLV
                     ILDEPSLGLSPKYVDIIFEQLLTLKKQGRTLLMVEQNAARALELSDRGYALELGHNRF
                     ADTGAKLLANEDVRRMYLGGA"
     gene            complement(8774..>9257)
                     /locus_tag="AC16FV_02450"
     CDS             complement(8774..>9257)
                     /locus_tag="AC16FV_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2848956.1"
                     /translation="ARTFQLSRVFPELTVLENLVVAGSRHDRSVRERALGLLERVHLR
                     EYAAVLGKNLSYGQQKLVEFLRVLMSDPALILLDEPAAGVNPTLRQTLWDMVRHLNGL
                     GTTFLVIEHNMEVIADLCSNVYVLSEGEIIAHGDFAAIRNNEQVLEAYFGKKRKSATA
                     "
BASE COUNT         1817 a         2878 c         2822 g         1740 t
ORIGIN      
        1 gcctgcaggc gcagctggcg cgctgggtgg gcgaggccgc ctatcagccg ctgcccgccg
       61 agctgcctcc cctgccggcc gtgcctgaaa ctttcgatcc cacccgccac cccgactggc
      121 tcgcggccca atacgggctg gaacaggcgc gcctcgaggt cgacatggcg cgccaggaat
      181 acaaaccggg atggctgctg gatctttcct acggtttccg ccgcccgatg ccgaatggca
      241 tggagcgacc ggacatggtg accgccatgg tgacgctcga tatgcccatc ttccgcgata
      301 agcgccagga ccggcgactg gcggaaaaac aggccatgga atccggcatg cgctacgaaa
      361 tcgaggacaa gcgccgcgag ctcgaggtca tgtaccgctc catgcgcgcg gagtatgacg
      421 cgctcgtcga gcgcgaaaaa atcttcgcgc aggaactgct gcccgccact cgccgcgagt
      481 cgcaggtcac cgtggccggg tttgcacgcg atcaggccga gtaccgcgag gcactcatga
      541 agacgcttga caccgaactg gaataccagc gcctgcgcgc cgatcgcttc aaggcgcagg
      601 cggaacttct gtatctcacc ggagaaccac agacatgaac cggaaaatga aactcaccgc
      661 gataatcggc gctgtcctgg tactgggcgt cgccgctggt tggtactttc tctattccga
      721 caagggccct gccggcgcgg gaggcgacac gaaagcggaa cgtcaggtac tttactggac
      781 cgaccccatg gtaccgggct atcgcagcga caagcccggc aagtctccgt acatggacat
      841 ggagctggtg ccagtctacg cggacgggac cggcgacggc ggcgccccgg tggtcacgat
      901 ccgtcctgag ttcatccaga atttcggggt gcgcacccac aaggtaacgc tgggcggtgc
      961 ggcacgcacg ctcgaggcgc atggctatct ttttcgcgaa agcggaagac tgctggtgct
     1021 ggtggatacc ttcgagcgtg acacctggat gcgcgcggga ctgcccgcgg aaatacgcgt
     1081 ggcggatttt ccgggacgcc aatggagcgg cacggtggaa agcgtgaaat ccgacgtgga
     1141 tatcggcacg cgctcgctca aggcgcgcat tcgcatcacc aacccggacg ccgcgctcag
     1201 ccccaacatg tacgctgatg tgatcatcag cgacaccgct cagcgcaatg ggaaaatcat
     1261 ggtgccgcgc gaggcgctga tccgtaccgg cgcccgcacc gccgtggtac tggcgctcgg
     1321 ttccggccgg ttccagccgg tggaagtcgt gccgggtgag gaatccggcg actgggttga
     1381 aatccgccag ggcataaagc aaggcgacac ggtcgtcacc tcgggccagt tcctgatcga
     1441 ctccgaggcc agcgtgcgcg ccagcttcca gcgcatggaa tccgcgcctg tcgatccgca
     1501 cgcgggacac aaggagaaaa aaccgtgatc gcccatatca tccgctggtc catccgcaac
     1561 cgcttcctgg tgctgttgct gaccgcgctt gtggtggcct gggggatttt tgccgtcagc
     1621 cgcacgccgc tcgatgcgat tcccgacctg tcggacgtgc aggtcattat ccagaccagc
     1681 tatcccggtc aggcgccgca ggtggtggag gatcaggtga cttatccgct cacgacggcc
     1741 atgctctcgg tgccgggcgc cactactgtg cgcggcttct cccagttcgg cgagtctttc
     1801 gtctacgtgc tgttcaagga cggcaccgat ctctattggg cgcgctcgcg cgtgctcgag
     1861 tacctgagcc aggtgttgcc gcgactaccg cgcggggcgc gaccggcgct cgggccggac
     1921 gccaccgggg tcggctgggt gtacgaatac gcgctggtcg accgcagcgg caagcacgat
     1981 ctggcgcaat tgcgcacgct gcaagactgg tttctcaaat acgagctgcg caccgtgccg
     2041 ggcgtggccg aggtcgcgac tgtcggtggc atggtcaagc agtatcaggt ggttgtggac
     2101 cccgtgaggc tgcgcgcctt ccggctcaca ctcatggatt tgcagcgcgc cattgaaaaa
     2161 gccaatcagg aaaccggcgg atcggtcgtt gaaatggccg aggccgagta cctggtgcgc
     2221 gtgcgcggat atcttcaaac taaaagcgaa ttgcgcgccg tgccgctggg cgtgacatca
     2281 aacggcacgc ccatccggct cgaaaacgtg gccgacatcc gcctcgggcc cgagatgcgc
     2341 cgcggcatcg ccgatctcaa cggcgaaggc gaagtggccg gcggcatcat cgtcatgcgt
     2401 catggcgaaa acgcgctggc cacgatcaac gcggtcaagg ccaggattgc aactctcaag
     2461 aacagtctac ccgagggcgt ggagatcata ccgacttatg accgctcaga ggtcatcaag
     2521 aacgctgtcc ataatctcac tgggacactg gtcgaggaat tcatcgtggt ggcgatcgta
     2581 tgcatggtgt ttttgtttca cctgcgctcc tcgctcgtgg tcatcgtcac gctgcccatc
     2641 ggcattctcg tcagcttcat catcatgaaa taccagggca tcaacgccaa catcatgtcg
     2701 ctcggcggca tcgccatcgc catcggcgcc atggtggacg ccgccatcgt catgatcgag
     2761 aacgtacaca agcgcatgga aaagcaagag gtcaccgatg aaaaccgctg ggccatcgtc
     2821 cgttccgcgg ccgaggaact cggaccaccg ctgttctatt cgctgctgat catcgcccta
     2881 agcttcattc cggtgttcgc gctcgaagcg caggaaggac ggctgttctc gccgctggcc
     2941 ttcaccaaga cctacgccat ggccgcggct gcggcgcttt cgatcacgct cgtgcccgtt
     3001 ctcatgggct atttcatccg cggacatatc cgcggtgaac aggaaaatcc cctcaatcgc
     3061 ggtctgatcc gcgcctaccg gccgatgctg gattgggtac tggcgcggcc caagcaggtg
     3121 ttgtggggcg cgcttgcgct ggtcgtattg ggcgctattc ccatcatgca actgggttcc
     3181 gagttcatgc cagacatgga cgaaggcgat attctgtaca tgcccaccac cctgccgagc
     3241 ctggcctctg gcaaggcgca gcaactcttg cagcagacaa accggatact caagaccttt
     3301 cccgaagtgg aaagcgtgtt cggcaaaagc gggcgggcgg aaacggcgac cgacccggcg
     3361 ccactgtcca tgtttgaaac cacgatacaa ctcaaaccgc gctccgagtg gcggccgggc
     3421 atgaccacgg ccaaactcat caaggagatg aacgcggcca ccaggtttcc gggcctgtcg
     3481 aacgccttcg tcatgccgat caagacacgc atcgacatgc tgaccaccgg cattcgcacc
     3541 cccgtgggcg tcaaggtttc cgggccgtcg ctcgccacgc tcgacagcat cggccgggag
     3601 atcgaaacag cattgcatga cgtgcccggc acggtgtcgg tttactccga gcgcgtgacg
     3661 gcggcacgct acgtggacgt acgcatcgac cggcatcgcg ccgcgcgctt cggcctcaac
     3721 atcgcggaca tccaggaaac cgtggccacc gccgtgggtg gcatgaacat caccgacacc
     3781 atcgaaggcc gcgaacgcta tccggtgaac atccgctacc cgcaggagat tcgcgactcg
     3841 ctcgcgcgcc tccaggcgct gccgctggta accatgagcg gtctgccgat cgcgctgtcg
     3901 gaagtcgccg acgtgcgcgt ggtggacggt cccgacatga tccgcagcga gaacgcgcgc
     3961 ctgtcgggct ggacttacgt tgacattcgc gaccgcgata tcggttccta cgtggccgac
     4021 gcgcaacgcg ccgttatgga aaaagtgaag ctgccgccgg gctattcgct ccactggacc
     4081 ggccagttcg agtacatgca gcgtgccgtc gaacgcctcg aggtcgtcat cccgctcacg
     4141 ctggtcatca tcctgctgct gctctatctc aattttcgca atggcatgga agtattgatc
     4201 gtgatcggta ccctgccgct cgcgctggtg ggcggtttct ggctgctgta tttcctgggc
     4261 tacaacctgt cggtcgcggt cgctgtgggc ttcatcgcgc tgggcggcgt ggcggtggaa
     4321 atcggcgtgg tgatgctggc ctacctcgat caggcgctca agcggcgcga aaccgaacgt
     4381 accgccaagg atcagcggtt gacgacagac gatgtgcgcg aagcggtgat cgaaggcgcg
     4441 ctgctgcgtg tgcggcccgt gaccatgacc aaggtcgcga tcatcgcggc attgctgccg
     4501 atattatgga gtcatggaac gggttcggag gccatgcagc gcatcgcggc acccatggtg
     4561 ggcggcatgc tgagcgttgc cattctgaca ctggccgtaa ttccagcggc ctatttcctg
     4621 tggcaggcct ggcgtgtcgg gaagagcgta aagaaaaact aactgcgctt gcgcacgtag
     4681 tagcggaaga ttttgccgtc gtcctctacc cggaggagtt catgaccggt gcgcttgcac
     4741 caggcgggaa tatccatgcg cgagccgatg tcggtggaga tcagttccag cacctcgccc
     4801 ggctgcatcg ccttgatcgc gcgatgcgcc tcgcgcaacg gaccgggaca gcattcgccc
     4861 gaggtgtcca ccgtgcggtc aggttcgttc tggctcacaa cccttctcct tcgctaaacg
     4921 gtcatcgcta tattagctca tcccgtcgcc ggggccggat ataatcccgc gccatgggca
     4981 gaaaccgtct tccgccggcc aaaagctcgg cctacatcac gccgcaaggc gcgcaacggt
     5041 tgcgcgagga gctggaccat ctgtggcgcg tgaagcgacc acaggtgaca caggccgtat
     5101 cggaggccgc cgcccagggc gaccgctcgg aaaacgccga atacatctac ggcaaaaaac
     5161 aattgcgcga gatcgaccgg cgcatacgtt ttttgcaaaa acggctcgac gtgctgaaga
     5221 tcgtggatcg gccaccgacc gacacctcgc gcgtcttctt cggtgcttgg gtacgactgg
     5281 aaagcgatac cggcgacatc gcggaatacc gcatcgtagg tccggatgaa tccgacatca
     5341 acaaggggct gatcagcatc gactcgccgc tggcccgtgc cctgctcaag aaagccgcgg
     5401 atgatgaagt gaccgtgtcg ctgccgcgcg gtcaaacggt atacatcgtt ctggaggtac
     5461 gctacacacc ttttgacaac acgggctgac gggtgggaac tgctaagccc gcaatcaggc
     5521 catcgccggc ctgccggcac cgtcggaacc tgatgcttgg tcgaatccgg ccagcttgag
     5581 gacatccatc tcgccgagtc gcgacccgaa attctccacc ggcagcggct cgccgaaaag
     5641 aaatccctgc atatcgtggc agccgatggt cttgagaaag cgcgcctgtt cgcgggtttc
     5701 gacaccttcc gcgagaatcc caagatgcaa gctttttgcc atgacggcaa tcgtggtggt
     5761 gatggcggca tcgccgcgat tacgcgtgac atcgcggatg aaagagccgt cgatcttgag
     5821 cagatcgatg gggaaacgcc gcagatagtt gagcgaggaa taaccggttc caaaatcgtc
     5881 gatggaaagc gtcacgccga tcttcttgag ctggtgcatc aggtccagcg cctgttcggc
     5941 gtcgtccacc agtacggtct cggtaatctc cagttccagc gagcgcggcg gcagtccgct
     6001 ctcatcgagc gcctggcgca cgctgttcaa caacgttccg gcgcggaact gcttcatgga
     6061 caggttcacg gacacgttca gggacttgtg gccagcgtcc tgccagcgcc ggccctgcaa
     6121 acaggcctgc tgcaacaccc actccccgac aggaatcacc aggccggtgt cctccagtaa
     6181 cgggatgaaa ttatccggca tgaccagccc acggctggga tgctgccagc gcagcagcgc
     6241 ctccgcgccc gtaaccgttc cggtggcggt gttgacccgt ggctggtaat aaatgagaaa
     6301 ttccctgttc tgcagcgcct ggcgcagacc cagctcgtac tcgaaactct cctgggcgcg
     6361 cgcctccatc tcctgcgtat aaaacacgta gcagttacgc ccggtctcct tggccgcgtg
     6421 catcgcggca tcggcgtcct tgagcagtcg ttggatgtcc tgcgtggccc cgggatagat
     6481 ggcaataccg atgctcgcgg tgacgataat ttcccggttg ccgactttca tcggttcggc
     6541 aaatttctgg atcagccggt tggccacctg cgcggcgtgt tcgttcgagg gcaggcgctc
     6601 cagaatcacg gtaaattcat caccgccgat gcggcaggcc gtgtccgagg cacgcaagca
     6661 ggtacgaatg cgtttcgcgg tctcgatcag aaccgcgtca ccgccttcgt ggcccagact
     6721 gtcgttgatg accttgaagc ggtcaacatc aatgaacatg actcccagcg atttctgcgc
     6781 gcggttggcc acggtgacgg cgtgctgaaa ccggtccttg aacaacgcgc ggtttggcag
     6841 gccggtcagg gcatcatggt aggccatgta atgcatctgc tcggcctgcg cccggcgctc
     6901 cgcgttctcg tgcgtgcgga tgatgcgatc cgcgcggcgc acgatcatga gcaggaaaag
     6961 ataaagcaga agcagaagga agatcacgcc gccaaaaacc gtgtattgcg tgcgctctat
     7021 gtccgccacc agcggcgtca cgtcggtgta gatttcgaaa accccctcga ccggtccacc
     7081 cggcgtgcga cggacgggag tataggatga caatagattg cggttttcta tgacctgctc
     7141 ggtggcggaa aaacgatcgc ggaacacgag atcactatcc gtgtgtccgg ctttggcctt
     7201 ctgaaagccg gagttgccgg ccttgttctc gccgatctgt ttcgcttccg tcgagtagac
     7261 ggtcagaccc gacatattgt atatcttcac cttgaccaca tgcagaccgc gaatcttctg
     7321 gagcacatcc tcccgcaggc tggccacttc cggttgcgat gccagttcgc gcacgggaat
     7381 gccggcggcg tgcacgataa agtccacgta tttgggccac aaggagttgg aaagcgactg
     7441 ggtcaggaaa acgttggcct gtgtttgttg gtcgatcagg gaacgcaccg caatgctgcg
     7501 ataaaaaaca cccagcgcca ccgccaccag aacgatgccg agaaaactcg cgatggaata
     7561 ggtgcgtgtg agacggaaca ttagcctaac cgcggtttaa agccattcaa acaagttctg
     7621 ttcaagcagc gtcgggaaga aactcgaatt cgacgagttc cattccctcg atcgaggtgc
     7681 attgcaccgc cacaccgtag tggcagacga tgtccctgtc cgcctgggac aaaatgtcga
     7741 cgctccctgc ttttttcctg ccgccggcca gcaccttcag gcgccgtgcc ttgcgctgct
     7801 ttcctgtccg ggtttccatg gccttctcct tatatgcctg tctgcccaaa gggattgcat
     7861 aggttgtgcc aaaacgcggg cgaccccccc gatttcggcg gaaatgcacc aaaacgggtt
     7921 cggggctgcc cggccaaggg gccgattttc gctctaggcg agaagaagaa acctcgtgaa
     7981 ttagcagctt ttccggtatc cggcccgaag gcctgcgcca tcaggcaaca gccggccaag
     8041 tccggcgcaa cgcagggtca ggccccgccg aggtacatgc ggcgcacgtc ctcattggcc
     8101 aggagcttgg cgccggtgtc ggcgaagcgg ttgtggccga gttcgagcgc gtaaccgcgg
     8161 tcggaaagtt ccagcgcccg cgcggcgttc tgttccacca taagaagagt gcggccctgc
     8221 ttcttgagcg tgagcagctg ctcgaaaata atgtcgacat acttcgggga aagtccgagg
     8281 gagggctcgt cgaggatcac gagacgcgga ttcatcatga gtgcgcgccc gatggcgagc
     8341 atctgctgtt cgccaccgga gagttttccc gccggctggt ggcgccgctc gtacaggcgc
     8401 gggaactgat cgaacaccgc ctgcaccgcc tcgcggcgct tgcgactatc gctttgcaga
     8461 aacgcgccca tctcgagatt gtcggacacg gtcatctggg cgaacacgat gcgcccttgg
     8521 ggaacgtagg tgatgcccaa tgccgggatc acatgcgccg gggaattcgt gatgggacgg
     8581 ccgtcgaaaa caacgctccc cttctgcacc ggctccaggc cgatgactgc cttgatgacc
     8641 gtggacttgc cggcgccatt gggtccgatg atcgacacga tctcgccggc atgcacctcg
     8701 atattcacgc cgaacaggac ctgcaccgcc ccgtacgaga cttccagatc gcgcacggtc
     8761 agcagtggtt cagtcatgcc gtagccgact tccgtttttt gccaaaatac gcttccagaa
     8821 cctgttcatt attgcgaatc gcggcaaaat caccgtgcgc gatgatttcg ccttcgctca
     8881 atacgtacac attggaacag agatcggcga tgacctccat gttgtgctcg atgacgagaa
     8941 atgtcgtgcc cagtccattc aggtgccgca ccatgtccca cagcgtctgc cgcaacgtcg
     9001 ggttgacccc agccgccggc tcgtcgagca ggatcagcgc cggatcgctc atgagcacgc
     9061 gcaggaattc gaccagcttc tgctggccgt aggaaagatt ttttcccagc accgcggcgt
     9121 actcgcgcag atgcacgcgt tcgagcaatc cgagcgcgcg ctccctgacg ctgcggtcgt
     9181 ggcggctgcc ggccacgacg agattttcca gcaccgtcag ttccgggaag acacgcgaga
     9241 gctgaaaggt gcgcgcc
//
LOCUS       DBNGZE010000096         3310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_429456, whole genome shotgun sequence.
ACCESSION   DBNGZE010000096 DBNGZE010000000
VERSION     DBNGZE010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_429456"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1313
                     /gene="purT"
                     /locus_tag="AC16FV_02455"
     CDS             114..1313
                     /gene="purT"
                     /locus_tag="AC16FV_02455"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR2848957.1"
                     /translation="MKIGTPLSPSATRVMLLGAGELGKEVIIALQRFGVEVIAVDRYP
                     DAPGHQVAHRAHVINMADGTALRALVEKERPHIIVPEIEAIATDMLAEIERDKLAEVI
                     PVARATQLTMNREGIRRLAAETLELPTSRYAFATSMTELKEAIDGGIGYPCIIKPVMS
                     SSGKGQSTVNGPDDVAKAWEYAMSGARVNQTRVIVEEMIKFDYEITQLTVRAVGDSGK
                     VETFFCDPIGHVQVKGDYVESWQPQAMSEKALMRSREIAKKVTDNLGGRGIFGVELFV
                     KGDHVWFSEVSPRPHDTGMVTMISQIQNEFELHARAILGLPVSVAMRAPGASAVIYGG
                     MEAKGIAFEGVDEALRVPQSDLRLFGKPEAFTRRRMGVALANGANTDEARARAKLAAS
                     KVRPVKG"
     gene            1420..1836
                     /locus_tag="AC16FV_02460"
     CDS             1420..1836
                     /locus_tag="AC16FV_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR2848958.1"
                     /translation="METCPLCHPESETVLWQDNRCRVILVDDADYPGFCRVIWGGHVK
                     EMTDLTEPERAHLMAVVFATEGALRDVLRPDKINLASLGNQVPHLHWHAIPRFTDDAH
                     FPDPVWAVRKRTARTTSLDRSKLVGELKQRLGDSLD"
     gene            1934..2287
                     /locus_tag="AC16FV_02465"
     CDS             1934..2287
                     /locus_tag="AC16FV_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR2848959.1"
                     /translation="MSTMPDDSQRQVDLRSLKNIATAVYALQAVSFFLGITYFIAIIL
                     DYVKLDDARGTWIESHFRWQIRTFWFGLLWFVVGGLTFFFIIGGIIIFANTLWLIYRI
                     LKGWLNLNDGKPMPV"
     gene            2323..3006
                     /gene="tsaB"
                     /locus_tag="AC16FV_02470"
     CDS             2323..3006
                     /gene="tsaB"
                     /locus_tag="AC16FV_02470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR2848960.1"
                     /translation="MSLTLKILALDTSTEACSAAVWTDGAVVERFESGSQHSERILAM
                     VDDLLAETGFALTQFDAISFGRGPGSFTGLRIGAGVAQGLAFGADIPVTPISSLAALA
                     QGVDESRVLAAFDARMNQIYSGAYVRNAQGVVELVGAESVLVPSDIPLPEGSNWVGAG
                     NGWDQYHAALLQRLGNRVANWLKQVSPRARDVGRLGAVAFRAGKAIAAEQALPVYVRD
                     DVAMKQVKS"
     gene            complement(3021..>3310)
                     /locus_tag="AC16FV_02475"
     CDS             complement(3021..>3310)
                     /locus_tag="AC16FV_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HIR2848961.1"
                     /translation="TPDDFTDTTLPKPLLPHRAKLYGLTISPERLAKIRDERKPGSQY
                     SSFENCRDEVRAAEALLRQAHVPYLDTTTMSVEEIAISILHATGLIKRLKS"
BASE COUNT          676 a          888 c         1024 g          722 t
ORIGIN      
        1 gatgaacacg gataaacaca gataaaatct caacagtaat ttgtttattc tgttctccgt
       61 gttcatctgt gtttatctgc ggttccataa atattatttt taggaaacaa ataatgaaaa
      121 tcggcacacc actgagcccc agcgccacgc gtgtcatgct gctgggagcg ggagaattgg
      181 gaaaagaagt catcattgcc ctgcagcggt ttggcgttga agtcatcgcg gtggatcgct
      241 atcccgatgc gccggggcat caggtcgcgc accgcgcgca cgtgatcaac atggccgacg
      301 gcaccgcctt gcgtgcgctg gtggaaaaag aacggccgca tatcatcgtg cccgagatcg
      361 aggccatcgc cacggacatg ctggcggaaa tcgaacgcga caagctggcc gaggtgattc
      421 ccgtggcgcg tgcgacgcag ctcaccatga atcgcgaagg tatacgccgt ctcgcggccg
      481 agacgctgga attgccgacc tcgcgttatg cctttgccac cagcatgacc gaactcaagg
      541 aggccatcga tggtggcatt ggttatccct gcatcatcaa gccggtgatg tcatcttcgg
      601 ggaaggggca atcgacggtg aacgggccgg atgacgtggc caaggcctgg gaatatgcca
      661 tgagcggcgc gcgcgtgaac cagacacgag tgatcgtcga ggaaatgatc aagttcgatt
      721 atgaaatcac gcaactgacg gtgcgcgcgg tgggcgattc cggaaaagtg gaaacctttt
      781 tctgcgatcc catcgggcat gtgcaggtca agggcgatta tgtcgagtcc tggcagccgc
      841 aggcgatgag cgagaaggcg ttgatgcgct cgcgcgagat cgcgaagaaa gtcacggaca
      901 atctcggcgg tcgcggcatt ttcggcgtgg aactgttcgt caagggtgat cacgtctggt
      961 tcagtgaggt cagcccgcgc ccgcacgata ccggcatggt gaccatgatc agccagatcc
     1021 agaatgagtt tgaattgcat gcgcgcgcca ttttggggtt gcccgtgtca gtcgccatgc
     1081 gcgcgccagg cgcgagcgcg gtgatttatg gcggcatgga ggccaagggc attgccttcg
     1141 aaggtgtgga cgaggcgctg cgcgtaccgc aatccgatct gcggttgttc ggcaagcccg
     1201 aggcgttcac gcgccggcgc atgggtgtgg cgctggccaa cggcgccaat accgatgagg
     1261 cccgtgcccg tgccaagctc gcggcatcga aggtcaggcc agtgaaagga tgaaaattga
     1321 gatagatagg attaacagga ttgaaaaaat atatttcaat ttttcttgtc tttagtttta
     1381 atcctgtaaa tcctgttaat cctgtccatt acttctttta tggaaacttg tccgttatgt
     1441 catcccgaaa gcgaaaccgt cctgtggcag gacaaccgct gccgcgtgat cctggtcgat
     1501 gacgccgatt atcccggatt ctgccgggtg atctggggcg ggcacgtgaa ggagatgacc
     1561 gatctcacgg aaccggagcg ggcgcatctg atggcggtgg tattcgccac cgaaggggcg
     1621 ctgcgcgacg tattgcggcc ggacaagatc aatctggcct cgctgggaaa tcaggtgccg
     1681 catctgcact ggcacgccat cccgcgcttc accgacgacg cgcactttcc cgatcccgtc
     1741 tgggccgtgc gcaaacgtac cgcgcgcaca acgtcgctag acaggtcgaa gctggtcggg
     1801 gaactcaagc aacgcctcgg tgacagtctt gattaagcca gaccggtagg ggcaatcgtc
     1861 tgttttcgct gttattctat agccgggtgg aaaacctcga taacgcctcg acaggcaata
     1921 ggagccgttc cgtatgtcca cgatgccaga cgacagtcaa cgccaagtcg atctccggtc
     1981 gctgaaaaat attgccacgg ccgtgtatgc tctgcaggcg gtgagttttt tcctcggcat
     2041 cacctatttc atcgcgatca tactcgacta cgtcaagctc gatgacgcgc gcggtacctg
     2101 gatcgaatcg catttccgct ggcagatacg caccttctgg ttcggattgc tatggtttgt
     2161 ggtgggtggg ctgacatttt ttttcattat tggcgggatc atcatcttcg ccaacacact
     2221 ctggctaatt taccgcattc tcaagggctg gctgaatctt aatgacggca aaccgatgcc
     2281 ggtctgagac gtcggcgtaa cgtccgtcgt tcgtatccat ccgtgtctct aactttgaaa
     2341 attctcgcgc tcgacacctc taccgaagcc tgttccgccg cagtctggac ggatggcgcc
     2401 gtggtggaac gattcgaatc cggcagccag cattccgaac gcattctcgc catggtggat
     2461 gacttgctgg cggagacggg atttgccctg acgcagttcg acgcgatttc cttcggccgc
     2521 gggccgggat cgttcacggg cttgcgcatc ggtgcgggcg tggcgcaggg cctcgccttc
     2581 ggcgcggaca ttccagtaac accgatctct tcgctcgcgg cgctggcgca gggagtagac
     2641 gaatccaggg tattggcggc tttcgatgcg cgcatgaatc agatttacag cggcgcttat
     2701 gtcagaaacg ctcagggtgt tgtcgaattg gtaggggctg aaagtgtgct cgtgccttct
     2761 gatattccat tacccgaagg cagcaattgg gtcggggccg gtaatggttg ggaccagtat
     2821 cacgccgcac tgctgcaacg cctaggcaat cgtgttgcta actggctcaa acaggtctcg
     2881 ccgcgcgccc gggatgtcgg ccggctcggg gccgtggcat ttcgggcggg caaggcaatc
     2941 gccgcggaac aagcgctgcc ggtctatgtg cgtgacgacg tggcgatgaa gcaggtgaaa
     3001 tcctgaacgg gcgacgctgc ctagctttta aggcgcttga ttaaccccgt ggcgtgcaga
     3061 atcgagatgg cgatttcctc gaccgacata gtggtagtgt cgagataagg cacgtgcgct
     3121 tggcgcagca gggcctcggc ggcacggacc tcgtcgcggc agttctcgaa cgaggaatat
     3181 tggcttcccg gtttgcgttc gtcgcggatt ttcgccagcc gctcgggaga gattgtcagc
     3241 ccatacagct tggcacggtg cggtagcagc ggcttcggta gcgtggtgtc ggtgaaatcg
     3301 tcgggcgtca
//
LOCUS       DBNGZE010000097         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_152096, whole genome shotgun sequence.
ACCESSION   DBNGZE010000097 DBNGZE010000000
VERSION     DBNGZE010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_152096"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16FV_02480"
     CDS             complement(<1..254)
                     /locus_tag="AC16FV_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR2848962.1"
                     /translation="MEVTAMYFKRKATENLQNEKLQHALKKMQSKFVDKRAEALMELP
                     NFTEIRDAATDIRERVIRNLDGYLLRFEQEASARGAVVHWA"
     gene            complement(245..982)
                     /locus_tag="AC16FV_02485"
     CDS             complement(245..982)
                     /locus_tag="AC16FV_02485"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR2848963.1"
                     /translation="MRVGLFVTCLVDLMRPSIGFAALRLLEVAGCEVVVPTAQTCCGQ
                     PGYNSGDNASAKALALKLLKEFDGCDYVVAPSGSCAGMIRVHYPALFKDDPAVIESIK
                     RLSARTYELTDFLVNVVKIKNIPGRYSGTLTYHDSCSGLRELNVQTQPRSLLAKMPGV
                     TLTEMQNATTCCGFGGTFSVKFGDISTRMADKKCASITQTGAGAVVAGDLGCLLNIEG
                     RLRRNGDLQTRVLHIAEILAGEGDTWK"
     gene            complement(1039..2220)
                     /locus_tag="AC16FV_02490"
     CDS             complement(1039..2220)
                     /locus_tag="AC16FV_02490"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071269 - L-homocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HIR2848964.1"
                     /translation="MSDDFDQWGFDTRAVRAGQHRTSEGENSEPIFATSSYVFKSAAE
                     AAARFSGEKPGNIYSRFTNPTVRTFEERLAALEGGERCVATGSGMAAILSTCLALLKS
                     GDHIVSSRNIFGPTVLLFNNYLGKFGIETSYVSLSDYGAWEKAIRPNTKLLFVETPSN
                     PLTEIADIARLAKLAHAKGCLLVVDNCFCTPALQLPLKLGADVVIHSATKYLDGQGRC
                     IGGALVGNKELVGEKVYGFMRTCGPSLSPFNAWVFLKGLETLSLRMKAHCANALELAR
                     WLEKQKRVQRVYYPGLASHPQHKLAKKQQSDFGGIVSFELKGGKDAAWKLIDSARVLS
                     ITANLGDTKSTITHPATTTHGRLTPEQRQDAGISDGLIRISVGLEDVEDLKKDLARGL
                     S"
BASE COUNT          511 a          763 c          708 g          502 t
ORIGIN      
        1 gcccagtgca ccaccgcgcc gcgcgcgctc gcttcctgct cgaagcgcag caggtaacca
       61 tcgagattcc tgatgacgcg ctcgcggatg tcggtcgcgg catcgcggat ttccgtgaaa
      121 ttgggcagct ccatcagcgc ctcggcgcgc ttgtcgacga atttcgactg catcttcttc
      181 agggcgtgct gcaacttttc gttttgcagg ttttccgtcg ccttgcgctt gaaatacatc
      241 gcggttactt ccatgtgtcg ccctctcccg cgagaatttc ggcaatatgc agcacccggg
      301 tttgcaggtc gccgttgcga cgcagccggc cctcgatgtt taaaaggcaa ccgagatcac
      361 cggccaccac ggcgcccgcc ccggtctggg tgatactggc acatttcttg tcggccatgc
      421 gcgtggaaat atcgccgaac ttgacggaga aggttccgcc gaagccgcaa caggtggtgg
      481 cgttctgcat ttcagtcagg gtcacgcccg gcattttcgc cagcaaggag cgcggttgag
      541 tctgcacgtt gagctcgcgc aagcccgaac atgaatcgtg gtatgtcagc gtgccggaat
      601 aacggccggg gatatttttg attttgacga cattgaccag aaagtcggtc aactcataag
      661 tgcgggcgga caggcgcttg attgattcga tgacggccgg atcatccttg aataaggccg
      721 ggtaatgcac gcggatcatg ccggcgcagg aaccggaggg tgcgacgaca taatcgcagc
      781 catcgaattc cttgaggagt ttcagcgcca aagccttggc ggaggcgtta tcgcccgagt
      841 tgtagccggg ctggccgcag caagtttggg ccgttggtac tactacttca caaccggcca
      901 cttccagcaa gcgcaaagcc gcgaatccga tgcttggccg catcaggtcc acgagacagg
      961 tgacgaacaa accaacgcgc atgatggcgg cattctacat ggtgtgccgc cgttttgttc
     1021 agcgcgtgcc gattcgcatc atgaaagccc gcgcgccaaa tccttcttga ggtcctccac
     1081 gtcttccagc cccaccgaaa tccggatcag gccgtcgctg atgccggcat cctgacgctg
     1141 ctccggcgtg agacgtccgt gcgtggtcgt agccgggtgc gtgatggtgg atttggtatc
     1201 gcccagattg gccgtgatgg aaagcacgcg cgcgctgtcg atcagcttcc aggccgcgtc
     1261 ctttccgcct ttcagttcaa acgacacgat gccgccgaaa tccgactgct gctttttggc
     1321 cagcttgtgc tgcgggtgcg acgccaaacc cggataataa acccgctgca cgcgcttctg
     1381 cttctccagc cagcgcgcga gttccagcgc gttcgcgcag tgcgccttca tgcgcagcga
     1441 aagtgtttcc agtcccttca ggaacaccca ggcattgaac gggctcagcg aaggcccgca
     1501 ggtacgcata aagccataga ccttctcgcc caccagttcc ttgttgccga ccagggcacc
     1561 accgatacaa cggccctgac cgtcgagata cttggtcgcg gaatggatca cgacatccgc
     1621 gcccagcttt aatggcagtt gcagcgccgg tgtacagaaa caattgtcca ccaccagcag
     1681 acagcccttg gcatgcgcca gtttcgccag ccgcgcgatg tcggcgattt ccgtcaacgg
     1741 attggacggc gtttccacaa acaggagctt cgtattcggc ctgatcgcct tctcccacgc
     1801 gccgtaatcc gaaagcgaga cataactcgt ctcgatgccg aacttgccga ggtaattgtt
     1861 gaacaacagg accgtcggcc cgaagatgtt gcgcgacgag acgatatgat cgccgctctt
     1921 gagcagcgcg aggcaggtcg agaggatcgc cgccatgccg gaaccggtgg cgacacagcg
     1981 ctccccgcct tccagcgccg ccaggcgttc ctcgaaggtc cgcaccgtgg gattggtgaa
     2041 gcgcgagtag atgttccccg gcttttctcc ggaaaaccgc gccgccgcct ccgccgcgct
     2101 tttgaacacg tagctggacg tggcaaagat cggctcggaa ttctcgccct cgctggtacg
     2161 atgctgcccc gcccgcaccg cgcgggtatc gaagccccat tggtcgaaat cgtcagacat
     2221 ggcctgctgc tcctgttatt acagttacgg cgcaggcttg cgggtgagcc acagaaacga
     2281 aaacggccgc aaatgcggcc ggaagcggga actctcgctt tagcggcatt tgtatcgcgc
     2341 ccgcaagctg aagctcaaat cggcgccaag taatacaaat ctataacctc gctaggcatc
     2401 tgtcaacgcg gacatcgata attcatcacg ctaactaagg ccagaattta atgtaaaccc
     2461 cataaactaa gaaaccaagc gaga
//
LOCUS       DBNGZE010000098         4589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_82897, whole genome shotgun sequence.
ACCESSION   DBNGZE010000098 DBNGZE010000000
VERSION     DBNGZE010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_82897"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16FV_02495"
     CDS             complement(<1..544)
                     /locus_tag="AC16FV_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR2848965.1"
                     /translation="MPFIPHTDADVKAMLASIGVKSIDDLFDEIPEKLRAGKLTQVPE
                     GLSEIEVTRLMQARANQDGAYINFIGAGVYEHHIPAAVWEIVTRGEFYSAYTPYQAEA
                     SQGTLQLLYEYQTMMASLTGMDVSNASLYDGASGLAEAVLMAVRAHKKARKILMPSTV
                     HPIYRRVVHAIVANQNIELVE"
     gene            complement(664..1059)
                     /gene="gcvH"
                     /locus_tag="AC16FV_02500"
     CDS             complement(664..1059)
                     /gene="gcvH"
                     /locus_tag="AC16FV_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR2848966.1"
                     /translation="MSNIPKDLKYTKSHEWVKLNGDGSVTVGITHHAQDLMGDMVFVD
                     LPQAGTKVTSGKECGVVESVKAASDVYAPVSGEIVEANAALSDSPETVNKDPYGSGWM
                     FRLKPANKAELDGLLDAKAYEALVASEKH"
     gene            complement(1075..2169)
                     /gene="gcvT"
                     /locus_tag="AC16FV_02505"
     CDS             complement(1075..2169)
                     /gene="gcvT"
                     /locus_tag="AC16FV_02505"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR2848967.1"
                     /translation="MGHRTPLYENHLKMGAKIVDFGGWDMPLHYGSQIEEHHKVRQDC
                     GMFDVSHMNVVDVKGSKVRDFFRLLLANNVDKLKAKGKALYSCMLTAKGGVIDDLIVY
                     YMDDQWFRVVMNAATRDKDMAWVNAAAKEFGGLTVTPRNDMAMIAVQGPNAKEKIYQA
                     LGEALRVPAGTLKPFHAVTVGELFIATTGYTGEDGFEIILPAKAAPFTWQMLHEAGVP
                     PIGLGARDTLRLEAGMNLYGSDMDETTTPLESGLAWTVALEPADRKFIGREVLDKQKA
                     QGVPRQLVGLVLEGKGVLRNHQKVMCNGAGDGEITSGSFSPTLEQSVAFARVPAAVKI
                     GDRCTVDLRGKPATVRVVKYPFVRNGKSCI"
     regulatory      complement(2366..2463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(2547..3416)
                     /locus_tag="AC16FV_02510"
     CDS             complement(2547..3416)
                     /locus_tag="AC16FV_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848968.1"
                     /translation="MLLVFGLTVEGQAAASKSSAKPCADPAANIELALISKSGPTTGR
                     VRITGVVKNLGKTAWTVTSPSQRPQMVLARKDAGTPPEGEPVQPAIAIRQLLPGQQYR
                     IDYQTDWDTDKKGPRPRFIARFINPGQAGRRGASSRQDCRSDNNRKEITATDIDRLFQ
                     RPTPVKPLVAQSYRLLGGIGVNTVETDLAYKRSSGNAGKLTASVAAPYTGTSDDVPIS
                     GNSGTEKIRVHVPCDAKHTSASNPPPIAITYRLWNSLSLPGRSGWVAGSSIEQAIPYR
                     ELCDPAVTNKTNP"
     gene            complement(3768..>4589)
                     /locus_tag="AC16FV_02515"
     CDS             complement(3768..>4589)
                     /locus_tag="AC16FV_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2848969.1"
                     /translation="RHEREQRYKLIGARAWRASAACAAVLLLAFIVPVGQLLHWAWGV
                     ARADFDLRYLRFFFNTVSLGAAAAVVTTFCALLLAYTYRLKPDRLVRGAVHFATLGYA
                     LPGSVLAVGIMVSFVWLDRQFAQWLQSLFGIAAGPLLTGSLLALLLAYGVRFMAVAHG
                     PIDSSFERIKPSLWQAARSLGASNREILWRVSIPLLRSGLLSAALLVFVEVMKEMPAT
                     LLLRPFGWDTLATRIFEMTSEGQWERAALPALTLVLAGLIPVIMLVRRSSSSPGY"
BASE COUNT          873 a         1406 c         1370 g          940 t
ORIGIN      
        1 tttccacgag ctcgatgttc tggttggcga cgatggcgtg cacgacccgg cggtaaatcg
       61 gatgcaccgt ggacggcatc agaatcttgc gcgccttttt atgcgcgcgc accgccatca
      121 gcaccgcttc cgcgaggccc gaggcgccgt cgtagagcga ggcgttcgag acgtccatgc
      181 cggtgagcga ggccatcatg gtctggtatt cgtagagcaa ctgtagcgtg ccctggctcg
      241 cttcggcctg atacggcgtg tacgcggagt agaactcgcc gcgcgtgacg atctcccaca
      301 ccgccgccgg gatgtgatgc tcgtagacac cagcgccgat gaaattgatg tacgcgccat
      361 cctggttggc gcgcgcctgc atcaggcgcg taacttcaat ttcgctcagg ccttccggca
      421 cctgtgtcag tttgccggcg cggagctttt cggggatttc atcgaacaga tcgtcaatcg
      481 acttgacgcc gatgctcgcg agcatcgcct tcacgtcggc gtcggtgtga ggaataaacg
      541 gcattttgta tttcctaaaa aatcaattca caggattgac aggattaaca agattaaaac
      601 taaaaaactt tataaattaa tcctgtaaat cctgaaaaat cctgttaatc ctgtctattt
      661 ctttcagtgc ttttccgaag cgaccagcgc ttcgtaggcc ttggcgtcga gcaggccgtc
      721 gagctcggcc ttgttcgcgg gcttcagacg gaacatccag ccggatccat aaggatcctt
      781 gttcaccgtc tcgggcgagt cggacagcgc ggcattggcc tcgacgatct cgccgctgac
      841 cggcgcatac acatccgaag ccgccttcac cgactcaacc acgccgcatt ccttgccgct
      901 cgtgaccttg gtcccggcct gcggcaggtc cacgaacacc atgtcaccca tgaggtcctg
      961 ggcgtggtgc gtgatgccga cggtaacgct gccgtcgccg ttcagcttca cccattcgtg
     1021 cgatttcgtg tacttcaggt cttttggaat attgctcatg gttttatctc acatttagat
     1081 gcagcttttg ccattgcgaa caaacggata tttgacgacg cgcaccgtgg cgggcttgcc
     1141 gcgcaggtcc acggtgcagc gatcgccgat cttgacggcg gcgggtacgc gcgcgaaagc
     1201 gacggactgt tccagcgtcg gcgaaaaact cccgctggtg atctcgccgt cgccggcgcc
     1261 gttgcacatc accttctggt gattgcgcag cacgccttta ccttccagca ccagccccac
     1321 gagttggcgc ggcacgcctt gggccttctg cttgtccagc acctcgcggc cgatgaattt
     1381 gcggtctgcc ggttccaacg ccacagtcca ggccaagccc gattccagcg gcgtggtggt
     1441 ttcgtccata tcggagccgt agagattcat gccggcctcg agccttaagg tgtcgcgcgc
     1501 gcccaggcca atcggcggca cgccggcttc gtgcagcatc tgccaagtaa acggcgccgc
     1561 cttggccgga agaatgattt caaatccgtc ctcaccggta taaccggtgg tggcgataaa
     1621 gagctcgccc acggtcacgg cgtgaaacgg cttgagcgtg ccggcgggaa cgcgcagtgc
     1681 ctcgcccaaa gcctgataaa tcttctcttt tgcattcggt ccctgcaccg cgatcatcgc
     1741 catgtcgttg cgcggcgtga cggtgaggcc gccgaattct ttcgcggcgg cattgaccca
     1801 ggccatgtcc ttgtcgcgcg tggccgcgtt catgacgacg cggaaccact ggtcgtccat
     1861 gtaatagacg atgagatcgt cgatcacgcc gcccttggcc gtgagcatgc agctgtacag
     1921 cgccttgcct ttggctttta gcttgtcgac gttgttggcc aagagcaggc ggaaaaaatc
     1981 gcgaaccttg cttcccttca cgtcgaccac gttcatgtgc gagacgtcga acatgccgca
     2041 gtcttggcgc accttgtggt gctcctcgat ctgggagccg tagtgcaacg gcatgtccca
     2101 gccgccgaag tcaacgatct tggcgcccat tttgagatgg ttttcgtata acggtgtgcg
     2161 gtgacccata aagacctata tgaaatccaa ccacagagga cacagagttt caaaaatttt
     2221 catctctgtg tcctctgtgg ttaagcttct taagaaaaat aaaaaggggc gatggccgtc
     2281 aggccatcgc ccctctgtcc ggtaacctga gagcttgagc cccttcggtg gatggcggtg
     2341 cgccatctct ctccagagtc gacctgtcgt ctccgcggtc cttttgcctg agcgattcct
     2401 ggcgattgcg ccttcggcgc cccgagtcat tacatatttc tgggtctctc ccgcgggtat
     2461 gggtcgaggg gcggcactat agcgcagcgt ctttacagcg ccaagcaggg aatttcccgc
     2521 cgaaacacgt gccgggcggg tcggatttac gggttcgttt tattcgtgac ggcggggtcg
     2581 caaagctcgc gataaggtat cgcctgctcg atactggagc cggccaccca gcccgatctg
     2641 ccgggaagac tgagcgaatt ccagagccgg taggtgatgg cgatgggagg cgggttcgaa
     2701 gccgatgtgt gtttcgcatc gcatggaaca tgcacgcgga ttttttccgt gccgctgttc
     2761 ccgctgatcg gcacgtcatc ggatgtgccg gtatagggcg ccgccacgga agccgtcagc
     2821 ttgccggcgt tgcccgagct gcgcttgtag gccagatccg tttccaccgt gttgacgcca
     2881 atgcctccca gcaggcgata gctctgggcc acgagcggtt tcaccggcgt gggccgctga
     2941 aacaggcggt cgatatctgt tgcggtgatt tccttgcgat tgttgtcgga gcggcaatcc
     3001 tgacggctgg acgcgccccg gcggccagcc tgtcccgggt tgataaagcg cgcgatgaaa
     3061 cggggtcgcg gacctttttt gtccgtgtcc cagtctgtct ggtaatcgat ccggtattgc
     3121 tggccgggca gcagttgccg gatcgcaatc gcgggttgca ccggctcgcc ctccggcggc
     3181 gtcccggcat ccttgcgcgc gagcaccatt tgcgggcgtt gtgatggact ggtcacggtc
     3241 catgcagtct tgcccaggtt cttcaccaca ccggtgatgc gcacgcggcc ggtggtgggt
     3301 ccggatttcg atatcagcgc cagctcgatg ttcgccgccg ggtcggcgca gggtttcgcg
     3361 ctggacttgg acgcagcggc ctgcccttca accgtcaggc cgaagacaag cagcatcgcc
     3421 gtacagcacc ccgttatggt cagtgtatat ttcatttttc tggctttatt cccggcccgg
     3481 gaacgaacgg cgcttggacc accccggtgc aaacacggga gatattacac ggcccgcccg
     3541 gagacggaaa atccggaggg attggcggcc gattattgcg gcggcacgaa tctagttgaa
     3601 catgtctatg ggtgttattc cctccaatcc gggatcgaag tcgtcaagcc gccgcaataa
     3661 tcagaaatct taatgatttt tgttattgac ccggcgtgcc cgtgccatca cggataccca
     3721 gtaacgtcta tcatggcctt ccttcgttca agaacaatca tgccgggtca ataacccggc
     3781 gaagaagacg atcgccgcac cagcatgatg accgggatca ggcccgccag caccagcgtc
     3841 agcgccggca gcgcggcgcg ctcccactgg ccttcggagg tcatctcgaa gatgcgcgtg
     3901 gccagcgtgt cccacccgaa cgggcgcaac agcagggtcg cgggcatctc tttcatcacc
     3961 tcgacgaaca ccagcagcgc ggccgacagc aatccgctgc gcagcagcgg aatcgagaca
     4021 cgccagagga tttcgcggtt gctcgcgccc aagctgcgcg ccgcctgcca tagcgacggc
     4081 ttgatgcgct cgaaactgct gtcgatcggg ccgtgggcca ccgccatgaa gcgcacgccg
     4141 taggcgagca gcagcgccag cagactgccg gtcagcagcg gcccggcggc gatgccgaac
     4201 agtgactgaa gccactgggc gaactgccgg tcgagccaga cgaaggaaac catgatcccg
     4261 accgccagca ccgaaccggg cagggcatag cccagcgtgg cgaaatgcac cgcgccgcgc
     4321 accagtcggt cgggtttgag gcgataggta taggccagca gcagggcgca gaacgtggtc
     4381 acaaccgccg cggcggcgcc gagcgaaacc gtattgaaga agaaccgcaa ataacgcaga
     4441 tcgaaatccg cccgcgcgac accccaggcc caatgcaaca actgccccac cggcacgatg
     4501 aacgcgagca gcaataccgc ggcacaggcg gcgctagcgc gccaggcacg cgcgccgatg
     4561 agcttgtaac gttgctcgcg ttcatgacg
//
LOCUS       DBNGZE010000099         4021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_916368, whole genome shotgun sequence.
ACCESSION   DBNGZE010000099 DBNGZE010000000
VERSION     DBNGZE010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_916368"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="AC16FV_02520"
     CDS             <1..981
                     /locus_tag="AC16FV_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR2848970.1"
                     /translation="RPRYFHLAGAKKYIDDMSLALQTEQQWSARREEANLLYVALTRA
                     RQILYVSGCEPGQGDRGWYGFIEKRLRQAGDSAEAARTGLKLNTISLDDGKTIFNTCA
                     SLEFGAPPTSLLLEQRSRGPGFVIDPLLMRPLPVMTETGILNPSRSVPVEDDHPDEAA
                     EFPDTRAEVQRRGVVIHRMLERLTSGDTRAAVEKKLRQEFGGSLEEKDFNNCWREACA
                     IADHEDFREFFDPARYREARNEIPILYRAGERDVYGVIDRLIMRDDEIVLIDYKTHAR
                     ATRGNIAQLAEDFREQMRQYGEGARRLWPDKKLRLLLLFTACGGVVELSP"
     gene            complement(983..2068)
                     /locus_tag="AC16FV_02525"
     CDS             complement(983..2068)
                     /locus_tag="AC16FV_02525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2848971.1"
                     /translation="MDQESTTQEREWVRLRKQLRDNERIWSGFRQIEVRMIGSHSLPD
                     LINIITRGIPETFPGIDCVTIACTDPEYEMTRLIEAGVEPDSGARSFVAISRETLDGL
                     FTRPWRPRLGPVDERLHSLLFSNHPCASGSVAIAPLIRRGELIGSLNQGSHDLRHFVP
                     GIATDLLEHLAAVAAMCIDNAINHERLKLDGLTDPLTGISNRRFFERRLAEEVERWAR
                     QAGPLVCMLVDVDFFKQVNDQYGHQVGDRVLRQIASLLGQDLRGSDVLARYGGEEFVL
                     LLPGTTPAQGATIAERLRSNIEQSAFVIPEGVNLDVTVSIGLACLQPGVDSYGPDPAV
                     WLFQQVDAALYQAKESGRNRVIQAVLS"
     gene            complement(2107..2877)
                     /locus_tag="AC16FV_02530"
     CDS             complement(2107..2877)
                     /locus_tag="AC16FV_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR2848972.1"
                     /translation="MMAGAMPSGNFIPARNYLMLRFFIICLACFVVPFSATAGEQEVK
                     QIKAELERAFPELKSASVKPSPVSGMYEVEHDSKIFYTTSDGKYLFMGDVMDLRAQTN
                     LTEARRAAIRTRLLDAVGEQNMIVIGPDKPKRTLTVFTDVDCGYCAKFHLDVPALNKQ
                     GVKVRYLFYPRTGIGSESYKRAVAVWCADDRVKAIGIAKARGKLDMKTCANPVESHYQ
                     LGQRLDVGGTPTIFLDDGKLLPGYVPAPRLLAILGLKS"
     gene            complement(2936..>4021)
                     /locus_tag="AC16FV_02535"
     CDS             complement(2936..>4021)
                     /locus_tag="AC16FV_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008048427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR2848973.1"
                     /translation="IVGCGDATEVIRSGVKVTVSCAEGDTGQVYEGEQKFRIDRISLD
                     SAHRPRTKIMMNVGNPDQAFENSFLPNDGVGLARLEFIINHSIQAHPRALLEYGKLSR
                     DEQRHIDQLTAGYTDRRAYFIGRLAEGIGTIAAAFYPKPVIVRLSDFKSNEYATLFGG
                     AGFEPREENPMLGLRGASRYYSESFRDSFAMECAALRRVREDMGLDNVVVMIPFVRSV
                     DEAKNVLAVMRAHGLERGRAGLKVYLMCEIPANALLAEEFLQHLDGFSIGSNDLTQLT
                     LGVDRDSGLVTGFDERNPAVLKLMETAIQAARKMGKYVGICGQAPSDYPEITAWLVRQ
                     GIESISLNPDSVIPMTRVVQQAEQALK"
BASE COUNT          800 a         1226 c         1210 g          785 t
ORIGIN      
        1 cgtccgcgtt attttcatct cgcaggggcc aaaaaatata tcgacgacat gagcctggcg
       61 cttcagaccg agcagcaatg gtcggcgcgc cgcgaagagg ccaacctgct gtatgtggcg
      121 ctcacgcgcg cccgacagat actttatgtc tcgggctgcg aacccggaca aggtgaccgc
      181 ggctggtatg gttttattga aaaacgtctg cgtcaggccg gcgattccgc cgaagctgcg
      241 cgcaccgggc tcaaactcaa tacgatttca ctggatgatg gaaaaaccat tttcaatact
      301 tgtgcctcgc tggaattcgg tgcgccgcca acatcgttgt tgctggaaca acgctccagg
      361 ggccctggct tcgttatcga tccattgttg atgcggcctc tcccggtaat gacggaaacc
      421 gggatactca atcccagccg ttccgttccg gtggaagacg atcacccgga cgaggccgcg
      481 gaattcccgg atacacgggc cgaagtgcag cgccgtggcg tggtgattca tcgcatgctg
      541 gaacgtctga ccagcggcga tacgcgcgcg gcggtcgaaa aaaaattacg acaggaattc
      601 ggtggctcac tggaagaaaa ggatttcaac aactgctggc gggaagcctg tgccatagct
      661 gatcatgagg atttccgcga gtttttcgat cctgcccgtt atcgggaggc acggaacgaa
      721 atcccgattc tctatcgtgc tggcgaacgc gatgtctatg gcgtcatcga ccgtctcatc
      781 atgcgggacg acgagatcgt gctcatcgat tacaagaccc acgcgcgcgc cacccgggga
      841 aacatcgcgc aactggcaga ggacttcagg gaacagatgc gccaatacgg cgaaggggcg
      901 cggcggctct ggccggacaa gaaactgagg ctgcttctgc tcttcaccgc ctgcggcggc
      961 gtggtggaac tgtcgccgta aatcaggaca acacagcctg gatcacgcgg ttgcgccccg
     1021 attccttggc ctgataaagc gcggcgtcca cctgctggaa cagccagaca gctggatcgg
     1081 gtccgtagga atccacgccc ggttggagac acgccagacc gatgctcacg gtaacatcga
     1141 gattgacccc ttccggaatc acgaaggcac tctgctcgat gttggaacgc agacgctcgg
     1201 caatcgtcgc gccctgggcg ggcgtcgtgc ccggcaggag cagcacgaat tcctcgccgc
     1261 cgtagcgcgc cagcacgtca ctgccacgca gatcctgacc gagcaggctc gcgatttgcc
     1321 gtagcacgcg atccccgacc tggtgaccgt actggtcatt cacctgcttg aagaaatcca
     1381 cgtccaccag catgcacacc agcggcccgg cctggcgcgc ccagcgctcc acttcttccg
     1441 ccaagcgccg ctcgaaaaac cgccggttgg aaattccggt cagggggtcg gtaagaccgt
     1501 cgagtttgag tctttcatgg ttgatggcgt tatcgatgca catggcagcg accgccgcca
     1561 gatgctccag caggtccgtg gcaatacccg gcacaaaatg gcgcaagtcg tgactgccct
     1621 ggttgaggct tccgatcagt tcgccgcgcc ggatcagcgg cgcgatcgcc accgaacctg
     1681 aggcgcaggg atgattcgag aaaagcaggg aatgaagccg ttcatcaacc ggccccaggc
     1741 gtggacgcca cgggcgggtg aagagtccat ccagcgtctc gcgcgaaatg gcgacaaaac
     1801 tgcgcgcccc tgagtctggc tcgacgccgg cctcgatcag gcgcgtcatc tcgtattcgg
     1861 gatcggtgca ggcgatcgtg acgcagtcga tgccgggaaa ggtctcgggg atgcccctgg
     1921 tgatgatgtt gatcagatcg ggaagcgagt gcgaaccgat catgcgcact tcaatctggc
     1981 gaaaaccgga ccagatgcgc tcgttgtcgc gcaactgttt gcgaagcctg acccattccc
     2041 gctcctgcgt cgtgctttcc tggtccatgc ctccgtctcg ccgtcgctgt cccggtggcc
     2101 cggcgatcag ctcttgagac caagtatagc gagcaaccgc ggcgccggaa catagcccgg
     2161 caacaattta ccgtcatcga ggaagatggt gggagttccg ccgacatcca ggcgctggcc
     2221 cagttgataa tggctttcca ccggattggc gcaggttttc atgtccagct tgccgcgggc
     2281 cttggcgatg ccaatcgcct tgacgcgatc atcggcgcac cacaccgcca ccgcgcgctt
     2341 gtagctctcc gagccgatgc cggtgcgcgg atagaacagg taacgcacct tcacgccctg
     2401 tttgttgagc gcgggtacgt cgaggtgaaa cttggcgcag tagccgcaat ccacgtcggt
     2461 gaacaccgta agcgtgcgtt ttggtttatc gggcccgatc acgatcatgt tctgctcgcc
     2521 cactgcatcc agcaggcgcg tgcggatagc cgcgcgccgg gcctcggtca ggttggtctg
     2581 cgcgcgcaga tccatcacgt cgcccatgaa gagatatttg ccgtcgctcg tggtgtagaa
     2641 aatcttggag tcgtgctcga cctcgtacat cccggaaacg ggcgagggct tgacgctcgc
     2701 cgacttgagt tcggggaagg cgcgctcaag ttccgctttt atctgcttca cctcctgctc
     2761 gccagcggtg gcggaaaaag gaacgacgaa acaggcaaga cagattatga aaaatcgcaa
     2821 catgagataa tttctcgctg ggatgaagtt tcctgatggc attgcgccgg ccatcatact
     2881 ggccggatgc ggtccgcgcc agcattagac ggtgtcgggc acgttcggtt cgtggctatt
     2941 tcagcgcttg ttccgcctgc tgcaccacac gcgtcatggg gatgacgctg tcgggattga
     3001 gcgagatgga ctcgatgccc tgtcgcacca gccaagcggt gatctcgggg taatccgacg
     3061 gcgcctgccc gcaaatgcct acgtacttgc ccatcttgcg cgcggcctgg atggccgtct
     3121 ccatgagttt cagcaccgcc gggttgcgtt cgtcgaaacc ggtcacgagc cccgagtcgc
     3181 ggtccacgcc cagggtcagc tgggtcaggt cattggaacc gatagaaaaa ccgtcaaggt
     3241 gctgtaggaa ttcctcggct aaaagggcgt tcgcgggaat ctcgcacatg aggtacacct
     3301 tgagccccgc cctgccacgt tcgagcccat gcgcgcgcat gaccgccagc acatttttcg
     3361 cctcgtccac gctgcgcacg aacggaatca tcacgacgac attgtccagc cccatgtcct
     3421 cgcgcacccg cctgagcgcc gcgcactcca tggcgaagct gtcgcggaaa gattccgaat
     3481 aataacgcga ggccccgcgc agcccgagca tgggattttc ctccctgggc tcgaaaccgg
     3541 cgccgccgaa aagcgtggcg tattcgttcg atttgaaatc gctcaggcgc acgataaccg
     3601 gcttgggata aaacgcggcg gcgatggtgc cgataccttc ggcgaggcgg ccgataaagt
     3661 aggcgcgacg atcggtgtag ccggccgtga gctggtcgat gtgccgctgc tcgtcccggc
     3721 tgagtttgcc gtattccagc agcgcgcgcg gatgcgcctg gatgctgtga ttgatgatga
     3781 attccaggcg cgccaatccc acgccatcgt tcggcaggaa ggaattttca aaggcctggt
     3841 cgggattgcc gacattcatc atgattttcg tgcgtggccg gtgcgcggag tccaacgata
     3901 tccggtcgat gcggaatttc tgctcgccct cgtagacctg tccggtatca ccctcggcgc
     3961 aactcaccgt cactttcaca ccgctgcgga tgacttccgt ggcgtctcca caacccacga
     4021 t
//
LOCUS       DBNGZE010000100         2002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_847008, whole genome shotgun sequence.
ACCESSION   DBNGZE010000100 DBNGZE010000000
VERSION     DBNGZE010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_847008"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..879)
                     /gene="bamJ"
                     /locus_tag="AC16FV_02540"
     CDS             complement(160..879)
                     /gene="bamJ"
                     /locus_tag="AC16FV_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR2848974.1"
                     /translation="MKTSFVFGLLVLSLSFNAWGKEPAGGLKTDKQKFSYTAGYQIGQ
                     NLKRQNLDLDSKTFSQGVQDAMANAKPRLKPEEMQAAVQNQQKKDMEKQMAVAKKNQE
                     AGQVFLEANKKKDGVVTLPSGLQYKVLTEGKGKQPKSMDTVVAHYRGTLINGTEFDSS
                     YQRNEPATFPVGGVIKGWQEVLPLMKEGAKWQVYIPSDLAYGPRGAGGAIGPNEVLIF
                     DIELVSVKEAAAEAESKKEEK"
     gene            complement(926..>2002)
                     /locus_tag="AC16FV_02545"
     CDS             complement(926..>2002)
                     /locus_tag="AC16FV_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR2848975.1"
                     /translation="ATRKEFPDGIPAYGTDALRFTFASLATQGRDIKFDLGRIDGYRN
                     FCNKLWNAARYVLMNTEGQDCGISGKVELNAVDRWIVSRQQEVAAEVEAAFKEYRFDL
                     AAQAIYSFIWHEYCDWYLELSKVVLTDTHSRPEQLRGTRRTLVQVLEAALRMLHPIMP
                     FITEEVWQRVAKLAGKTGATLMREPYPRPEKNLIDAGASEEMRWAMAVITGVRNIRGE
                     MDISPGKPLPLMFQDGSPTDKKYLEANRPYLATLARAESITWLDKGREAPESATVLVG
                     HMKVLIPLGSLIDKKAELDRLGKEMDKIEKDLSKARTKLANPDFVARAPKNVVEQEQG
                     RVQQFETALVNLRNQRAKVEALPG"
BASE COUNT          341 a          611 c          589 g          461 t
ORIGIN      
        1 tctcccctcc ggggagaggg ttagggtgag gggcgcaacg ccgcgccatc gcacggcttc
       61 taccctcatc cccgccctct ccctcaaggg agagggagtt tacgtaagac ggcatgcgga
      121 cagaaacgcg ccatgcccac acgaaaccct gaagaacctc tatttttctt cctttttgga
      181 ttccgcttcg gcggcggctt ccttcacgga aaccagctcg atatcgaaga tcagcacttc
      241 gttcgggcct atcgcaccac cggcgccgcg cgggccgtag gcgagatcgg acggaatata
      301 aacctgccac ttggcgcctt ctttcattag cggcagcact tcctgccagc ccttgatgac
      361 gccgccgacc gggaaggttg cgggctcatt gcgctggtag gagctgtcga actcggtgcc
      421 gttgatgagc gtgccgcgat agtgagcgac gacggtatcc atgctttttg gctgcttgcc
      481 cttgccttcg gtgagaacct tgtattgcag tccgctgggc agcgttacca cgccgtcctt
      541 tttcttgttc gcttccagga agacttggcc ggcttcctgg ttctttttcg ccacggccat
      601 ctgcttttcc atgtctttct tctgctggtt ctggaccgcc gcctgcatct cctcgggctt
      661 gaggcgcggc ttggcgttgg ccatggcgtc ctgcacgccc tggctgaagg tcttggaatc
      721 caggtcgagg ttctgacgct tcaggttctg tccgatctgg taacccgcgg tgtagctgaa
      781 tttctgtttg tccgtcttga gaccgccagc cggttccttg ccccaggcgt tgaaggaaag
      841 ggagaggacg agcaggccga atacaaaact ggttttcatg acgactcctt gtggtgaata
      901 ttgggaacaa tgacgcgccg ccggctcagc ccggcaacgc ctcaaccttg gcgcgctggt
      961 tgcgcagatt gaccagcgcc gtttcaaatt gttgcacgcg gccctgctcc tgctcgacca
     1021 cgttcttcgg cgcgcgcgcg acgaaatccg ggttggccag cttggttctg gccttcgaca
     1081 ggtccttttc gattttatcc atttccttgc cgagacgatc caattccgct tttttatcga
     1141 tcagcgagcc gagcgggata agcaccttca tgtgaccgac caaaaccgtg gccgattcgg
     1201 gcgcctcccg ccccttgtcg agccaggtga tggattcggc ccgcgccaac gtcgcgagat
     1261 aaggacggtt ggcctcgaga tattttttgt cggtcggcga accgtcctga aacataagcg
     1321 gcagcggttt gccgggcgaa atgtccatct cgccgcggat attgcgcacg ccggtaatca
     1381 cggccatggc ccagcgcatt tcctcgctgg ccccagcgtc gatcaggttc ttttccggtc
     1441 gtggataagg ctcgcgcatg agggtggcgc cggttttgcc cgcgagcttc gccacgcgct
     1501 gccagacttc ctcagtgatg aagggcatga tcgggtgcag catgcgcagc gcggcttcca
     1561 gcacctgcac cagcgtccgg cgcgtgccgc gcagctgttc cggccggctg tgagtatccg
     1621 tcagcacgac cttggagagt tccaggtacc agtcgcagta ctcgtgccag atgaagctgt
     1681 agatcgcctg cgcggcaaga tcgaaacgat attccttgaa cgcggcttcc acctcggccg
     1741 cgacttcctg ctggcgcgaa acaatccagc ggtcgacggc attgagttcc accttgccac
     1801 ttatcccgca atcttggccc tcggtgttca tcagcacgta gcgcgcggcg ttccatagct
     1861 tgttgcagaa gttgcgatag ccgtcaatgc gaccgaggtc gaatttgatg tcgcgccctt
     1921 gtgtcgcaag gctggcgaag gtaaagcgca gcgcatcagt gccataggcg ggaatgccgt
     1981 cgggaaattc cttgcgcgtg gc
//
LOCUS       DBNGZE010000101         4262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1124844, whole genome shotgun sequence.
ACCESSION   DBNGZE010000101 DBNGZE010000000
VERSION     DBNGZE010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1124844"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC16FV_02550"
     CDS             complement(<1..260)
                     /locus_tag="AC16FV_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR2848976.1"
                     /translation="MKKLLILLVMVGLPAGAFAAGGHKSCGAVECDAVDIDLGDKASL
                     QRGARIFVNYCLSCHSAGYMRYNRMGRDLGISDELVKENLLFA"
     gene            complement(257..1510)
                     /locus_tag="AC16FV_02555"
     CDS             complement(257..1510)
                     /locus_tag="AC16FV_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR2848977.1"
                     /translation="MQKLLAWIDERFPLTKVWEEHLSKYYAPRNFNFWYYFGSLALLV
                     LVMQIVTGIFLTMHYKPDTELAFASVEYIMRDVDWGWLIRYIHSTGASFFFIVVYLHM
                     FRGLLYGSYRGPRELIWILGMVIFVALMAEAFMDYLLPWGNMSYWGAQVIISLFDAIP
                     LVGPTLSEWIRGDFVVSDATLNRFFAFHVIAIPLILVALVFVHIVALHKVGSNNPDGV
                     EIKKKKGPDGIPLDGIPFHPYYTVKDIVGVTVFLMLFAAVIFFAPELWGWFLEPDNFV
                     PANILQTPPEIVPLWYFTPFYSILRANVYNFLWIDAKIWGVIFMGLSIVLFFFLPWLD
                     RSPVKSIRYRGVLYKTALAIFVVSFIILGYLGTKSPEVISLFWFKNVYWVQAFTVLYF
                     AFFFLMPIYTKLDKTKPVPERVTWK"
     gene            complement(1510..2109)
                     /gene="petA"
                     /locus_tag="AC16FV_02560"
     CDS             complement(1510..2109)
                     /gene="petA"
                     /locus_tag="AC16FV_02560"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR2848978.1"
                     /translation="MSEEGVNPRRRRLLAITSGLGVIGTAFAVTPFVLSMLPSARAKA
                     AGAPVEVDISKLEAGQLLTVEWRGKPVWILRRTPAMLKSLTEVEPLLTDPKSGVTSQQ
                     PSYAQNEGRSIKPEILVLVGICTHLGCSPDKRLEAGVASGLGDAWKGGFFCPCHGSKF
                     DLAGRVFKNVPAPTNLVVPPHAYLSDTRIIIGADQKGAA"
     gene            2393..3562
                     /locus_tag="AC16FV_02565"
     CDS             2393..3562
                     /locus_tag="AC16FV_02565"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR2848979.1"
                     /translation="MISRHTLSYLIQAVILGLAIAFIVMYFWPQIANHVAPTVEIRQA
                     APETSQRPANGPYSYAQAVDRASPAVVNINTAKVVTVRPHPFFDDPVFRQFFGGGENL
                     ITPRKRIESSLGSGVIMSEQGYILTNHHVIHGADAIQVSLQDGRMVQAKVVGSDPETD
                     VAVLKIDLKRLPVMTLGHSDRLRVGDVVLAIGNPFGVGQTVTMGIVSATGRNKLGINT
                     FENFIQTDAAINPGNSGGALIDAEGNLVGINTAIFSRSGGNQGIGFAIPTSLAQNVME
                     EIIEHGRPLRGWLGIEAQVITPQIARALDLENTIGVVVVGVVRGGPAHRAGLQPGDVL
                     VAIDGKKITEAREALLTISGRKPGSRVKMEILREGKSMTLEATAIERPAQAAVTE"
     gene            complement(3616..4164)
                     /locus_tag="AC16FV_02570"
     CDS             complement(3616..4164)
                     /locus_tag="AC16FV_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848980.1"
                     /translation="MDKKLWTPVLLPVLLTAYPACADIFQDQITKSFRGFVIMDYGEF
                     DPDIRKNLDSNPAFITGDFNYDKYEDFAALIRDTVMKHYVAGERSYDYYETKLVACHG
                     LGNRKFNCTALFSGVTVLPEFHYLVKHSPGKTKCRSSQGDPVEAETEFIGWASTKGWA
                     TGSGETQYIYQLGGSYLQCGAN"
BASE COUNT          955 a         1280 c         1241 g          786 t
ORIGIN      
        1 gcgaacagca ggttctcctt caccagctcg tcgctgatgc cgagatcccg gcccatgcgg
       61 ttatagcgca tgtagcccgc gctgtggcag gacaggcagt agttgacgaa aatgcgcgcg
      121 ccgcgctgca acgaggcctt gtcgccgaga tcgatatcga cggcgtcgca ttcgaccgcg
      181 ccacaggatt tgtgtccgcc cgcggcgaac gcaccggccg gcaggccgac catgaccaac
      241 agtatcagga gctttttcat ttccaggtca ccctttccgg caccggcttg gtcttgtcta
      301 gtttggtgta aatcggcatc aggaagaaga aggcgaagta caggaccgtg aaagcctgga
      361 cccagtaaac gttcttgaac cagaacagac tgatgacttc cggactcttg gtgccgaggt
      421 aaccgaggat gatgaagctc accacgaaga tggccagcgc ggtcttgtac aacacgccgc
      481 ggtagcggat ggatttgacc ggggagcgat ccagccacgg gaggaagaag aacagcacga
      541 tcgacagtcc catgaatatc acgccccaga tcttggcgtc gatccagagg aagttgtaca
      601 cgttggcgcg caggatcgag tagaacgggg tgaaatacca cagcggcacg atctccggcg
      661 gcgtctgcag gatgttggcc ggcacgaagt tgtcgggttc gaggaaccag ccccacagct
      721 ccggcgcgaa gaatatcacg gccgcgaaaa gcatcaggaa caccgtcaca ccgacgatgt
      781 ccttcaccgt gtaatacgga tggaacggta tgccgtcgag cggaatgccg tccggcccct
      841 tctttttctt gatctcgacg ccatcggggt tgttcgagcc gaccttgtgc agggccacga
      901 tatgcacgaa caccagcgcc accaggatca gcgggatggc gatgacgtga aatgcgaaga
      961 agcggttgag cgtggcgtcg gacaccacga agtcgccgcg gatccattcg gacaacgtcg
     1021 ggcccaccag cggaatcgcg tcgaacagcg aaatgatcac ttgggcgccc cagtaggaca
     1081 tgttgcccca cggcaacaga tagtccatga aggcctcggc catgagcgcg acgaagatca
     1141 ccatgccgag aatccagatg agttcacgag gaccacggta ggagccgtag agcagaccac
     1201 ggaacatgtg caggtacacg acgatgaaga agaacgacgc gccggtcgaa tgtatgtaac
     1261 ggatcagcca gccccagtcc acgtcgcgca tgatgtactc gacggaggcg aaggccagct
     1321 cggtgtccgg cttgtaatgc atggtgagga aaatgccggt gacaatctgc atcaccagca
     1381 ccagcagcgc gagcgacccg aagtaatacc agaaattgaa attcctgggc gcgtagtatt
     1441 tcgaaagatg ttcctcccag accttggtca gggggaagcg ctcgtcaatc caggccagca
     1501 gcttttgcat caggcagctc ctttctgatc agcgccgata atgatgcgcg tatcggagag
     1561 ataggcatgt ggcggcacca ccaggttggt gggcgccgga acgttcttga acacgcggcc
     1621 ggccaggtca aacttggagc cgtggcaggg acagaagaac ccgcccttcc acgcatcacc
     1681 gagacccgag gcgacaccgg cctcgaggcg cttgtcgggc gaacaaccga ggtgcgtgca
     1741 gatgccgacc agcacgagga tttccggctt gatggagcgc ccttcattct gtgcatagga
     1801 aggctgttga ctggtcacgc cggatttggg atcggtcaga agcggttcaa cctcggtgag
     1861 cgacttcagc atcgcggggg tgcgacgcag gatccatacc ggcttgccgc gccattcaac
     1921 cgtaagcagc tgtccggctt cgagcttgct gatgtccacc tcgaccggcg caccggcagc
     1981 cttggcgcgg gcgctcggga gcatgctgag gacgaagggg gtgaccgcaa atgccgttcc
     2041 aatgactcct aatccgctgg tgatcgccag caagcgccgg cgacgcggat tgacaccctc
     2101 ttcactcatg aaaccccctc aattctcgtg gagcaaaaaa taaaaaaatc acgcacagag
     2161 gcgtaatttt atggaataaa tcagccgatg gagcgaataa aacccgtctg gccctgaaaa
     2221 acggcgcata ggattatata tcaaccctgt cggagtcaag cgcttaaatc aagtcgcgca
     2281 acattttttt acaaaaaaac ccgcggataa ccgccatatc gtcgtggtac ggcacgtgga
     2341 ccgggacttc ggataaaatc cctcgcccca tatcccgatt tgcgcatcga acatgatttc
     2401 gcgtcatacc ctgtcctatc tcatccaggc cgtcatcctc gggctggcca tcgccttcat
     2461 cgtgatgtat ttctggccgc aaatcgccaa tcacgtcgcg ccgaccgtcg aaatccgcca
     2521 ggccgcgccc gaaacctcgc agcgccctgc caacggcccc tactcctacg cgcaggcagt
     2581 ggaccgggcc tcgcccgcgg tcgtcaacat caacaccgcc aaggtcgtca ccgtccggcc
     2641 gcacccgttt ttcgacgatc cggtgttccg tcagttcttc ggtggcggcg agaacctgat
     2701 cacgccgcgc aagcgcatcg aatccagcct cggctccggc gtcatcatga gcgagcaggg
     2761 ttacatcctc accaaccatc acgtcatcca cggcgccgac gccatccagg tatccctgca
     2821 ggacgggcgc atggtgcagg ccaaggtggt gggcagcgac ccggaaacgg acgtcgcggt
     2881 gttgaaaatc gatctcaagc ggctgccggt catgacgctc ggtcactccg accgcttgcg
     2941 cgtcggcgac gtggtgctcg ccatcggcaa ccccttcggc gtgggccaga ccgtgaccat
     3001 gggcattgtc agcgccaccg ggcgcaacaa gctcggcatc aacaccttcg agaacttcat
     3061 ccagaccgac gccgccatca atcccggcaa ttccggcggc gcgctgatcg acgccgaggg
     3121 caacctggtc ggcatcaaca ccgccatctt ctcgcgcagc ggcggcaatc agggcatcgg
     3181 cttcgccatt cccaccagtc tcgcccagaa cgtgatggag gagatcatcg agcacggccg
     3241 cccgctgcgc ggctggctcg gcatcgaggc gcaggtcatt acgccgcaga tcgcgcgcgc
     3301 cctcgatctg gaaaatacga taggcgtggt ggtggtcggc gtggtgcgcg gcgggccggc
     3361 acatcgcgcg ggcctgcagc cgggcgacgt gctggtcgcc atcgacggca agaaaatcac
     3421 cgaggcacgc gaggccttgc tgaccatttc cggccgcaag cccggcagcc gcgtaaagat
     3481 ggaaatcctg cgcgagggaa aatcgatgac gctggaagcc accgccatcg aacgcccggc
     3541 gcaggcagcg gtgacggagt aagccgcggg atggtcagaa aagatacggg gctgaatacc
     3601 gcgcccgccg gattttcagt tggcgccgca ctgcaggtag gagccgccca actgataaat
     3661 gtattgggtt tctccgcttc ccgttgccca gcctttggtc gaagcccatc caatgaattc
     3721 ggtttcggcc tcgacaggat cgccttggct tgacctgcat ttcgtttttc ccggcgagtg
     3781 ctttaccagg taatgaaatt ccggcagtac cgtgacacca ctgaataatg ccgtgcagtt
     3841 aaatttcctg ttacccagac catggcacgc taccagcttg gtctcgtaat aatcgtagga
     3901 gcgttcaccg gcaacatagt gtttcatgac ggtatcgcgt attaacgccg cgaagtcctc
     3961 gtatttgtcg taattgaaat ctccggtgat aaaggcggga ttggaatcca gatttttccg
     4021 gatgtccgga tcgaactcgc cataatccat gatcacgaat ccgcggaagg atttggttat
     4081 ctgatcctga aaaatatctg cgcaggcagg gtaagccgtc agcaagaccg gcagcaaaac
     4141 cggcgtccac aatttcttgt ccattattac ttctccctgc ggacacgacc gtgatcccca
     4201 gaatacacct gctgtggatg tcacgcatgt cattgcctgg cggcatttcc acaacacatg
     4261 gg
//
LOCUS       DBNGZE010000102         4401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1819788, whole genome shotgun sequence.
ACCESSION   DBNGZE010000102 DBNGZE010000000
VERSION     DBNGZE010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1819788"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..874
                     /locus_tag="AC16FV_02575"
     CDS             188..874
                     /locus_tag="AC16FV_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR2848981.1"
                     /translation="MKRFGVYTIIAVFAIFSAGCATDEYGNRRPLTEAEKGAIIGATS
                     GAVIGALAKKDKRTKGALIGAVGGGLAGAAVGTYMDSQKKDLEKVLAPEVQAGAINVA
                     KVDQNNLLITMTAQTAFDFDSTEIKPGFHSTMDKIANVLVRYGKTHLTVVGHTDNVGT
                     QQYNQTLSERRSGAVADYLRVKGVIPQRLESVGRGENSPRATNATEEGRRLNRRVEIV
                     VEPVVAETQG"
     gene            complement(894..1964)
                     /gene="metA"
                     /locus_tag="AC16FV_02580"
     CDS             complement(894..1964)
                     /gene="metA"
                     /locus_tag="AC16FV_02580"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetA"
                     /protein_id="HIR2848982.1"
                     /translation="MPLVANSKLPTFERLRQEGENVLPGDVALHQDIRELHIGLLNMM
                     PDAALAATERQFFRLIGESNQIAQFYMHPFTLKELKRSAEAETYIGQYYESFDQIREQ
                     GLDALIVTGANVTQPDLALEPFWKPLNKVIDWAYDNVTSTLCSCLTTHAVLQFRYGKK
                     RRHLGFKRWGVYSHRVVDRKHPLVSDVNTRFDVPHSRFNQIDREQFEAAGLHILVESD
                     VAGVHLAASEDRFRLVFFQGHPEYDTVSLFKEYKREVNRFVRGEREDYPPFPENYFSL
                     LTEAMLDEHRDMLLAAKARGKPIPELPESIIVPNLDNTWHDTAEAVINNWIGKVYQVT
                     NNDRKKPFQDDVNPDDPLGLRR"
     gene            complement(1979..2818)
                     /locus_tag="AC16FV_02585"
     CDS             complement(1979..2818)
                     /locus_tag="AC16FV_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR2848983.1"
                     /translation="MKLSARDFRAWDNKCITLLGMSGVGKTRLAIKLRKRNWFHYSGD
                     YRIGTRYLDEAILDHIKQQAMQVPFLRELLRSDSIYIRNNITVDNLQPVSSFLGKLGN
                     PEKGGLGLKEFKHRQELHRQAEIATMRDVPEFIGKAREIYGYKHFVNDAGGSLCELDD
                     PATIKALADHTLILYIRATEKDEQELIHRAEEDPKPLYYREAFLDEQLGVYLREKKLS
                     YVAQIDPDDFVRWMFPRLFHSRVPRYQAIADQHGYTLSTDELKEVRDEAGFLRLVEKV
                     LER"
     gene            2966..3412
                     /locus_tag="AC16FV_02590"
     CDS             2966..3412
                     /locus_tag="AC16FV_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR2848984.1"
                     /translation="MTTIIPISLAGEVKITPENKCSYCKGATCCTYLTQPLDTPRSME
                     DFDLLLWQISHVNTQMYKDEDGWFLLVNNPCRHLQPGGRCGIYEHRPQICRDHSNDDC
                     EFEGPSGHEDFELFFPDYESLLDYCRRKFKNWDGRVLRKNAGQKKK"
     gene            3497..>4401
                     /locus_tag="AC16FV_02595"
     CDS             3497..>4401
                     /locus_tag="AC16FV_02595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR2848985.1"
                     /translation="MDQTIYDRLAKRLGRVHLRQRLGLETEYEAQVFHRNTHFFHLEN
                     WYFAHALIRGSLGLIGLHGRGRRNALKIRVVRNEVLFAALPAVFDGYTILHLSDLHLD
                     ISEGFVHALIERVRAVDYDLCVLTGDYRYRTRGPYEPALEGLARLRPHLKGPVYAVLG
                     NHDTIRMVPGMEDLGIQVLLNESVRLDRHGAAIHLAGIDDAHYYRVDNIHKAAHDIPH
                     EAVSILLSHTPETYRNAAHADFKLMLCGHTHGGQICLPGGIPLITDADCPRAFARGPW
                     RYQDLIGYTSVGAGSSIVDVRLNCLP"
BASE COUNT          849 a         1280 c         1316 g          956 t
ORIGIN      
        1 acgatggcga gctggtcatt tcgcgcagcc cgctcggtgg taccgcggtc gaagtgcggt
       61 tgtaactgaa ataaaaaaat tgacaggatt tacaggatta tttctaaaat tttaaaatcc
      121 ttaatcctgt aaatcctgaa aaatcctgtt aatcctgtct atttcgttaa aattggaggt
      181 tcgtatcatg aaacgttttg gcgtttatac gattattgcg gtgtttgcga tattttccgc
      241 cggttgtgcc accgacgagt acggcaatcg ccgccccctg accgaggcgg agaagggcgc
      301 cattatcggc gccacatcgg gcgcggtcat cggcgcgctg gcgaagaagg acaagcgcac
      361 caagggcgca ctgatcggcg cggtcggcgg tggccttgcc ggcgcggccg tgggcaccta
      421 catggattcg cagaaaaagg atctggaaaa ggtgctggct cccgaggtcc aggctggcgc
      481 tatcaatgtc gcgaaggtgg atcagaataa tctgctcatc accatgacgg cgcagaccgc
      541 gtttgatttc gactcgaccg agatcaagcc cggctttcac agcaccatgg acaagattgc
      601 caacgtgctg gtgcgctacg gcaagacaca tctgactgtc gtcggccaca ccgacaacgt
      661 cggcacgcaa cagtacaatc agaccctgtc ggagcgccgt tccggcgcgg tggcggatta
      721 cctgcgcgtc aagggcgtca tcccgcagcg gctcgaatcc gttggacggg gagagaactc
      781 gccgcgcgcc accaacgcca ccgaagaagg acgtcgcctc aatcggcgcg tcgaaatcgt
      841 ggtggagcct gtcgtcgccg aaactcaggg ttgagtggcg gatttaataa gcgtcaacgt
      901 cgcagaccga gcggatcgtc cgggttcacg tcgtcctgaa acggcttttt gcggtcgtta
      961 ttggtgacct ggtaaacctt gccgatccag ttattgatca cggcctcggc agtgtcgtgc
     1021 caggtgttgt cgagattggg cacgataatg gactccggca gctccggaat cggcttgccc
     1081 cgggccttgg ccgccagcag catgtcgcgg tgttcgtcga gcatcgcctc ggtcaggaga
     1141 ctgaagtagt tttccgggaa tggcgggtaa tcttcgcgct cacccctgac gaagcggttg
     1201 acctcgcgct tgtattcctt gaacaggctc accgtgtcgt attccgggtg tccctgaaaa
     1261 aaaaccaggc ggaagcgatc ctcgctcgcg gccaggtgca cgcccgcgac gtcgctttca
     1321 acgaggatat gcagccccgc ggcctcgaac tgttcgcgat cgatctggtt gaagcgcgag
     1381 tgcggcacgt cgaagcgcgt atttacatcg gacaccagcg gatgtttgcg atccaccacg
     1441 cggtgggaat agacccccca gcgcttgaat cccaggtgcc ggcgcttctt gccatagcgg
     1501 aactgcagta ccgcgtgcgt cgtcaggcac gagcacagcg tggacgtgac gttgtcgtag
     1561 gcccagtcga tcaccttatt gagaggtttc cagaacggct cgagcgccag gtccggctgg
     1621 gtgacgtttg cgccggtgac gatgagtgca tccagtccct gttccctgat ctggtcgaag
     1681 ctctcgtaat actggccgat ataggtttcc gcttcggcgc tgcgcttgag ttccttgagc
     1741 gtgaacgggt gcatgtagaa ctgcgcgatc tggttcgact cgccgatgag cctgaagaac
     1801 tgacgctcgg tcgcggccag cgccgcgtcc ggcatcatgt tgagcagccc gatgtgcaac
     1861 tcgcggatat cctgatgcag cgcgacgtcg cccggcagaa cgttctcacc ctcctgccgc
     1921 aggcgttcga atgtgggtag tttggaattg gcgaccagtg gcatggcggg accggggatt
     1981 atctttccag gactttttcc accagacgca ggaagccagc ctcgtcgcgc acttccttga
     2041 gttcgtcggt gctgagggtg taaccgtgtt gatcggcgat ggcctggtaa cgcggaacgc
     2101 gagaatgaaa cagacgcggg aacatccagc gcacgaaatc gtccggatcg atttgcgcca
     2161 cgtaggataa cttcttctcg cgcaggtaaa ccccaagttg ctcgtcgaga aatgcctcgc
     2221 ggtaataaag cggctttggg tcctcctcgg cgcggtgaat cagttcctgc tcgtcttttt
     2281 cggtcgcgcg gatatacagg atgagcgtat ggtcggccag cgccttgatg gtggcaggat
     2341 cgtcgagttc gcacaggctg ccgccagcgt cgttgacgaa atgcttgtaa ccataaatct
     2401 cgcgtgcctt gccgatgaat tccggcacgt cgcgcatggt cgcgatctcc gcctgccggt
     2461 gaagttcctg acggtgtttg aattccttga gaccgagccc gcccttttcc gggttgccga
     2521 gcttgccgag aaaactcgac accggctgga gattgtccac ggtgatgttg ttgcggatgt
     2581 agatcgagtc cgatcgcaac agctcgcgca ggaacggcac ctgcatcgcc tgctgcttga
     2641 tgtggtcgag gatggcctcg tcgagatagc gcgtgccaat gcgatagtcc ccggaataat
     2701 ggaaccagtt gcgcttgcgc agcttgatgg ccaggcgcgt cttgccgacc ccggacattc
     2761 cgaggagggt gatgcacttg ttgtcccagg ccctgaaatc cctggcgctc agtttcatgt
     2821 tggtgttctt cgccggtggt ctgcttattg tatgacagga atggtggaac cgcgcgccgg
     2881 cgcttgcggt tttcgcctgg ctcgtcatcc ccggaggtcg tcgctataat ccccgccctt
     2941 ccgggaccga cctgactgaa aacccatgac caccattatt ccgatctcgc tcgcgggcga
     3001 ggtgaaaatc acccccgaga acaaatgcag ctattgcaag ggcgcgacct gttgcaccta
     3061 cctgacgcag ccgctcgaca cgccgcgatc gatggaggat ttcgacctgc tactgtggca
     3121 gatctcgcat gtgaacaccc agatgtacaa ggatgaggac ggctggttcc tgctggtcaa
     3181 caatccctgc cggcatctgc agccgggcgg gcgctgcggc atctacgaac atcgtccgca
     3241 gatctgccgc gaccattcca acgacgattg cgagttcgag ggcccgtccg gccacgagga
     3301 cttcgagttg ttctttcccg attacgaatc gttgctggat tactgccgca ggaagttcaa
     3361 gaactgggac ggccgagtgc tccggaaaaa cgccggccag aaaaagaaat agggcgtttc
     3421 cgcgcgggac gtcccccgtt gaatctgcta accttgtaga cgtcggtatc ccttttccat
     3481 cgagtgtcac ccgcggatgg accagaccat ttacgatcgc ttagccaagc gcctcgggcg
     3541 cgtgcatttg cgacagcgcc tgggccttga gacggaatat gaagcgcagg tttttcatcg
     3601 gaacacccat ttctttcatc tcgagaactg gtactttgct catgccttga tccgcggcag
     3661 tctgggcctg atcggcctgc acggccgcgg ccgtcgcaac gcgcttaaaa tccgtgtcgt
     3721 ccgcaacgaa gtgttattcg cagccttgcc cgcggttttc gacggttaca ccatccttca
     3781 tttgagcgat ctgcacctcg atataagcga gggattcgta catgccctga tcgaacgcgt
     3841 ccgtgcggtg gattacgatc tctgcgtgct taccggtgac tatcgttacc gtacccgcgg
     3901 tccgtatgag ccggcactcg agggcctcgc gcgcctcagg ccgcatctca aggggccggt
     3961 gtatgccgtt ctcggcaatc acgacaccat tcgcatggtg ccgggcatgg aggatctggg
     4021 aatccaggtg ctcctcaacg aatcggtgcg gcttgatcgt cacggcgctg cgattcacct
     4081 cgcgggaatt gatgacgcgc actactatcg ggtggacaac atacacaagg ccgcgcacga
     4141 catcccgcac gaggcggtct cgatcctgct gtcgcacacg cccgagacct accgcaacgc
     4201 cgcgcacgcc gatttcaagc tcatgctctg cggccatacc cacggtggcc agatctgttt
     4261 gccgggcggt attccgctta ttaccgacgc cgactgcccg cgcgcctttg cgcgtgggcc
     4321 atggcgttac caggatctta ttggttatac ctcggtgggt gcgggcagct cgattgtgga
     4381 cgtgcggctc aactgtctgc c
//
LOCUS       DBNGZE010000103         5104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_430599, whole genome shotgun sequence.
ACCESSION   DBNGZE010000103 DBNGZE010000000
VERSION     DBNGZE010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_430599"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16FV_02600"
     CDS             <1..326
                     /locus_tag="AC16FV_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848986.1"
                     /translation="CYPSRPWGNEGKISSKLGSPCLAKNSGLAFHRLQSLLRSKAQGA
                     PSMAHPFAGTTIHRIVVYFRLTLGKTIRRFCYFAFFLNEVMFRKWSSPHPSQRHCAIS
                     TRFRG"
     gene            266..1624
                     /gene="xseA"
                     /locus_tag="AC16FV_02605"
     CDS             266..1624
                     /gene="xseA"
                     /locus_tag="AC16FV_02605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR2848987.1"
                     /translation="MEQPSPVTTPLRDIYTVSRLNREAKAVLEGGFPPIWIEGELSNL
                     SRPASGHVYFSLKDAQAQVRCAFFRQHQRLLGIALKDGLHVLARARVSLYEGRGDYQL
                     IIEYLEEAGEGALRRAFDVLKQRLLQEGLFDVAHKKPLPKLPRRLGIITSPSGAVLHD
                     ILTTLRRRFPGIAVLVYPVPVQGEGAAEKIAGAIRFAGKHGDCDALILARGGGSLEDL
                     WAFNEEVVARAIYACPIPIVSGVGHETDFTIADMIADVRAPTPTAAAEMLSPDQQEWL
                     AQFARLEARLITGVQGRLRSLQQHLDWLSARLVHPRNRIVLLQQSLKTLAQRLYLAQT
                     TSLRHARSELQAMIARLYQHTPRPRVQALMLDNRHLRSRLVIAIKRRLELAESRLRQH
                     MQSLHTLSPLGTLERGYAIVQQPDTGAVVTDAGKVRPGERVRARLARGRLDCLVEKTH
                     ED"
     gene            1614..2465
                     /locus_tag="AC16FV_02610"
     CDS             1614..2465
                     /locus_tag="AC16FV_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR2848988.1"
                     /translation="MKIRIWPLLAVLFSVPLQAQTLPRENPVPGGIVILPVAPDTESA
                     PVVHYDNQRVLVMPHAGKWQAVVGLPLSLTPGPQAVQVGGPENRRRDIAFIVRPKDYA
                     EQRLTIKEKRMVEPSAEDLARIERDQEVIRRAFTTWTDLPLKSLQFSLPARGRISSVF
                     GLRRFFNNEPRQPHSGIDIAGPAGTPVAAPLAGTVVEIGDYFFNGKTVFIDHGQGLVS
                     MFNHLSRVLVERGAHVNTGEKIGEIGMTGRVTGPHLHWTVSLNNSRVDPALFLPQEIL
                     DSKNSQD"
     gene            complement(2730..4892)
                     /locus_tag="AC16FV_02615"
     CDS             complement(2730..4892)
                     /locus_tag="AC16FV_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2848989.1"
                     /translation="MSAFHKSALRAGYRLAEYTIESVLGHGGFGITYLAHDTSLGAQV
                     AIKEYLPHEIANRENKTQVLPNPERDAMRDYQTGLKNFVKEARALAKFKHPNIVRVLR
                     FLEANGTAYMVMEYEEGQSLADYLKRNGPRLDEQTLLRVFIPILNGLHAVHEAEMLHL
                     DIKPENIYLRKDGSPILIDFGSARQGSVHMHKVALTHGYAPMEQYPDKGTPGPSTDVY
                     AIGASMYRCVTGKKPDDSLDRYEAVLKYQVDPLTPAVKAGGNSYQRNILECIDWALQV
                     YPRDRPRSAREFQDALMGKGGPSRSSSSSSNISQPATFKRSSSANAYRRPPVTTRKTQ
                     KDSSSAAVAWMAAGLMVASLAVAGFVYWADIKMHLDLFENPPADATTSPGANKPAAIS
                     EQPVEIAPTTAAPAVLPAEPAKPASKSVAHASTFPSVLTKTITGHQDWVQSVAFAPDG
                     KWFASAGLDKTIRLWVAATGTPLGTLRQSYAVNAIAISAGGKWLASAGDDGTVRLWEA
                     KSGTPRGALSGPGYAVYAVTFSPDGKLVAAAGKDRLVFVWDVNDGRRVQTLEGHTGDI
                     YSLAFSPDGKSLASAGADRTLIVWNVAHGQEAIRMTGHKDKILSLAWSPSGKWLASGD
                     AGQAIRLWDANTGTYIRTLSNNQAVLALAFSPDSRWLAAGLAGKTIQMFDAGDGAVMQ
                     ALAGHQDHVQSLAFSPDGSQLVSGSRDKTLRLWKAKNP"
BASE COUNT          962 a         1623 c         1490 g         1029 t
ORIGIN      
        1 cctgttatcc gtctcgccct tggggcaacg agggcaaaat cagttctaag ctcggctctc
       61 cctgcctcgc taagaactca ggtcttgcct ttcatcgctt gcaatccctg ctgcgctcga
      121 aagcccaggg tgcgccctcc atggcgcacc cgttcgccgg gacgacgatc caccggatcg
      181 tcgtctattt ccggctcacc cttggcaaga ccattcggcg attttgttat tttgcatttt
      241 tcctaaatga ggttatgttc agaaaatgga gcagccctca cccgtcacaa cgccattgcg
      301 cgatatctac acggtttcgc ggctgaatcg cgaggccaag gccgtgctgg aaggagggtt
      361 tccgccgatc tggatcgagg gcgagctttc caatctctcc cgtcccgcct cgggccatgt
      421 ctacttctcg ctcaaggacg cccaggcgca ggtgcgctgc gccttcttcc gccagcatca
      481 acgcctgctc ggtatcgcgc tcaaggacgg gctgcacgtg ctggcgcgcg cccgcgtcag
      541 tctgtacgaa ggacgcggcg actatcagct catcatcgaa tacctggaag aagccggcga
      601 aggcgccctg cggcgcgcct tcgatgtgct caagcagcgt ctgttacagg aagggctgtt
      661 cgatgtcgcg cacaagaaac ctctgcccaa gctcccgcgc cgtctcggca tcatcacctc
      721 tccgtccggt gcggtgctgc atgacatcct gacgacattg cgtcgccgct ttccggggat
      781 cgccgtgctg gtgtatccgg tgccggtgca aggcgaaggc gcggcggaaa aaattgccgg
      841 cgcgatccgg ttcgccggca agcatggaga ttgcgatgcg ctgatcctgg cgcgcggcgg
      901 cggttcgctc gaagacctgt gggccttcaa cgaagaggtc gtggcgcgcg ccatttatgc
      961 ctgcccgatt ccgatcgtga gcggcgtcgg acacgaaacc gacttcacca tcgccgacat
     1021 gatcgccgat gtgcgcgcgc caacgcccac ggctgcggcc gaaatgctga gccccgatca
     1081 gcaggagtgg ctggcgcagt ttgcgcgcct cgaagctcga ctgatcaccg gcgtgcaggg
     1141 acgcctgcgc agccttcaac agcatctcga ctggctcagt gcgcgcttgg tgcacccgcg
     1201 taaccggatc gtgctcttgc agcaaagtct caagaccctc gcccagcggc tttacctagc
     1261 acagaccacc agcctgcgcc acgcgcggtc ggagttgcag gccatgatcg cacgactata
     1321 ccagcacacg ccgcgcccac gcgtgcaggc gctgatgctc gacaaccgtc atttgcgttc
     1381 acgtctcgtc atcgccatca agcgccgttt ggagctggcc gagtcgcgcc tgcgacagca
     1441 catgcaaagc ctgcatactc tgagtccgct tggcacgctg gaacgcggtt acgccatcgt
     1501 gcaacaaccg gacaccggcg ctgtcgtaac cgatgccggc aaagtcaggc cgggcgaacg
     1561 cgtgcgtgca cgactggcgc gcggccggct ggattgccta gtggaaaaaa ctcatgaaga
     1621 ttaggatttg gcctttgctg gccgtgctgt tcagcgtacc gcttcaggct caaaccttgc
     1681 cgcgcgaaaa tccggtgcca ggcggcatcg tcatcctgcc ggtggcgccg gacactgagt
     1741 ccgcgcctgt ggtgcattac gacaatcagc gcgtgctggt catgccgcac gccggaaagt
     1801 ggcaggcggt ggtcggtttg cctttatcgc ttacgcccgg cccacaagcg gttcaggtcg
     1861 gcggcccgga gaaccgacgg cgcgatattg cctttatcgt gcgacccaag gattatgccg
     1921 agcagcgcct gaccatcaag gaaaaacgca tggtcgaacc ttcggcggaa gatttggcgc
     1981 gcatcgagcg cgatcaggaa gtcatccggc gcgcgttcac cacctggacg gacctgccac
     2041 tgaagtcgct gcaattctca ctgccggcgc gcggtcgcat cagcagcgtc ttcggattgc
     2101 ggcgtttctt caacaacgag ccgcgccagc cgcacagcgg catcgatatc gcgggaccgg
     2161 ccggcacgcc cgtggcggcg ccgctggcgg gaaccgtggt ggaaatcggt gactattttt
     2221 tcaacggcaa gaccgttttc atcgaccatg gccagggact tgtcagcatg ttcaaccacc
     2281 tgagccgcgt cctggtcgag cgcggcgcac atgtgaatac aggcgagaaa atcggtgaga
     2341 tcggcatgac cggacgcgtc accggcccgc atctgcactg gaccgtcagc ctcaacaaca
     2401 gccgcgtcga tccggcgctg ttcctgccac aagaaatttt ggacagcaaa aattcacagg
     2461 attaacaggg cgaagcggag tggtcgccga tgtacttaac gtacatcggc gccgctgagc
     2521 gcccgcacag ggatgtgcag gccggggttt atcggagcct ctgtcgcgac gccagggaag
     2581 gcatgccagc cgtccctggc gcaaccacac aggcttcgac atactcggcc ccgccatcct
     2641 tggcggggcg ctcgccggga cgaagacacg ttaagtgtct tcgtctattt ccggcttgcc
     2701 ctgtgaatcc tgttaattct tttttattat cagggattct tggccttcca cagccgcagg
     2761 gtcttgtcgc ggctaccgga aaccaactgc gacccgtccg gcgagaacgc cagagactga
     2821 acatgatcct gatgtccggc gagcgcctgc atcaccgcgc catcgccggc atcgaacatc
     2881 tggatggttt tgccggcgag gcccgcggcc agccagcggc tgtcgggcga aaaagccagc
     2941 gccaaaaccg cctggttgtt cgacaatgta cgaatatacg tgccagtgtt cgcatcccac
     3001 aggcgtatcg cctgtccggc gtcgccggag gccagccatt ttccgctcgg cgaccatgcc
     3061 agcgacaata tcttgtcctt gtggccggtc attctgatcg cttcctggcc atgggccaca
     3121 ttccatacga tcagggttct gtccgcgccg gcggaggcca gcgatttccc gtccggcgaa
     3181 aaggccagcg aataaatgtc tccggtgtgc ccttccagcg tctgcacgcg tcgtccgtcg
     3241 ttcacgtccc acacaaacac caaccggtcc ttgcccgccg cagcgacaag ttttccatcc
     3301 ggggaaaagg tcacggcata aaccgcgtaa cccgggccgc tcagcgcacc gcgcggtgta
     3361 cctgacttgg cttcccataa tctcacggtg ccatcatcgc cagcggaggc cagccatttt
     3421 ccgccggcag aaatggcgat ggcattcacg gcataactct gacgcagcgt gccgagcggt
     3481 gttcccgtgg cagcgaccca gagccggatg gtcttgtcga gaccggccga ggcaaaccat
     3541 ttgccatctg gcgcgaatgc gacggactgc acccaatcct gatgaccggt aattgtcttc
     3601 gtcagcacgg aaggaaaggt cgaggcatgg gccacggatt tcgacgccgg cttggcaggc
     3661 tcggccggga gtaccgcagg ggcggcggtc gttggcgcga tttccacagg ctgttctgat
     3721 attgcggcgg gtttattcgc gcccggcgat gtcgtcgcat cggccggtgg attttcaaac
     3781 agatccagat gcatcttgat atccgcccag tacacaaaac ccgccacggc caggctggcc
     3841 accatcaggc ccgccgccat ccaggccacg gctgccgagc tcgaatcctt ttgcgttttg
     3901 cgcgtggtaa ccggtgggcg ccggtaggca ttcgcggatg acgaccgttt gaaggtcgcg
     3961 ggctggctga tgttgcttga actcgagctt gagcgcgaag gaccgccttt ccccatgaga
     4021 gcatcctgga attcacgcgc cgaccggggc cggtcacggg gatagacctg caaggcccag
     4081 tcaatgcatt ccagaatatt acgttgatag gaatttcccc cggccttcac cgccggagtc
     4141 agcggatcta cctggtattt caacacggct tcgtaacgat ccagcgaatc gtccggtttc
     4201 ttgccggtga cacaacggta catcgaggcg ccgatcgcat aaacgtcggt agatggaccc
     4261 ggcgtgccct tgtccggata ctgctccatt ggcgcatagc catgggtcag agccacttta
     4321 tgcatgtgga ccgaaccctg gcgcgccgaa ccgaagtcaa tcagtatcgg gctgccgtcc
     4381 ttgcgcagat aaatattttc cggcttgatg tccagatgca gcatctcggc ctcatgtacc
     4441 gcatgcaggc cattcagaat cgggatgaaa acgcgcagca gcgtctgctc gtccaggcgc
     4501 gggccgttgc gcttgagata atccgcgagg ctctgcccct cctcgtactc catgaccatg
     4561 taagccgtgc cgttggcctc gaggaaacgc agcacgcgca cgatgttggg gtgcttgaat
     4621 ttcgccagcg cccgtgcctc cttcacgaaa tttttcaggc cggtctggta atcgcgcatc
     4681 gcatcgcgct cgggattcgg gagcacctgg gtcttgtttt cgcgattggc gatctcgtgc
     4741 ggcaggtatt ccttgatggc tacctgtgcg cccagcgacg tgtcatgcgc aagataagtg
     4801 attccgaagc cgccatggcc cagaaccgat tcgattgtgt attccgccaa ccggtaaccc
     4861 gcccggaggg cgctcttgtg aaacgcgctc acaccacggc cccgtcgtgt ccctgaccct
     4921 gaaactccat tttttccaac accatatata taagaatttt atttatcagt tctgaatcac
     4981 ttttcagtat agatcacgcg gatttcacga gcggcgccgc tttgtccggc gcccacccct
     5041 gccaccgccg gtttttttac cttgatacgg gttctcctcc tgacggcact cgatcagcac
     5101 cggc
//
LOCUS       DBNGZE010000104         5412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_917219, whole genome shotgun sequence.
ACCESSION   DBNGZE010000104 DBNGZE010000000
VERSION     DBNGZE010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_917219"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC16FV_02620"
     CDS             <1..463
                     /locus_tag="AC16FV_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2848990.1"
                     /translation="GRRVPLEDYLVGVVNDWLRTRRSVSVQRHFEGPRPSPEIIAEQT
                     LSQAIVNILNNAADSSPDNVEVSARWSEQELVLEIGDRGQGLTPETARAVGQPFFTTK
                     APGQGLGLGLFLAHATLRRLGGSVQMYNREGGGVCTQLVLPLVNLRVDGTS"
     gene            460..1011
                     /locus_tag="AC16FV_02625"
     CDS             460..1011
                     /locus_tag="AC16FV_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2848991.1"
                     /translation="MNTENAADKPSLLLVDDDETFTRVLSAALERRGFSVRIARDVAE
                     GFAAAESDLPEYAVVDLKMPGPSGLELVKKLKELDAHTRIVVLTGYASVATAVEAIKL
                     GAIHYLAKPADADEIVAAFQRDVGNTAVAVETKSLPLSRLEWEHIQKVLTESGGNISE
                     TARRLGMHRRTLQRKLAKRPVKA"
     gene            complement(1180..1827)
                     /gene="hflD"
                     /locus_tag="AC16FV_02630"
     CDS             complement(1180..1827)
                     /gene="hflD"
                     /locus_tag="AC16FV_02630"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HIR2848992.1"
                     /translation="MSTAYTDRIVALAGLFQAARLAQQLAREGRAEAPALAASVQSLL
                     IIDAPTTESVYGGAQGIKPGLELLRNKLAGGNTDASDVEIARYVIAMIHLEGQLRRRP
                     EMQDAVRRGIEAIREQMKFFETAENEETVHPRLVEKLAELYTQSISTLTPRIMVNGEH
                     GHLSNPSIAAKVRASLFAGIRSAFLWHQLGGSRWQLLFSRKKIADEAAKILEEKN"
     gene            complement(1935..2195)
                     /locus_tag="AC16FV_02635"
     CDS             complement(1935..2195)
                     /locus_tag="AC16FV_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR2848993.1"
                     /translation="MVARITEAMVYDALRKVIDPELKHNVVDLGFIQEMDIVDDYVHL
                     DIQLTTPYCPFAENIVAEIKRAVEGVQGVKRVEVERACMKED"
     gene            complement(2312..2752)
                     /locus_tag="AC16FV_02640"
     CDS             complement(2312..2752)
                     /locus_tag="AC16FV_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2848994.1"
                     /translation="MVTLGHVVFYVRDLEKSTAFYRDVVGLELAGRIFNGRAAMLTGG
                     STHHELLLIEIGAAPGPLQGRRIGLYHVGWKVGDNLDDLRRARDRVHAHGHDIEGMSD
                     HTVSQSLYLRDPDGNEIELFVDDPGVDWRHAHEWMEAPVKPLRL"
     gene            2882..4030
                     /locus_tag="AC16FV_02645"
     CDS             2882..4030
                     /locus_tag="AC16FV_02645"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR2848995.1"
                     /translation="MADKPRVGLILAGGGARAAYQVGVLKAIAEMLPRDCPNPFPILC
                     GTSAGAINATALAIYATRFHEGVRRLAYVWRNFNVRQVFRADTLGVLANGMRWFLATM
                     LGGLGRRNPRALLDRAPLRRLLQQTMPCDRIQASIDAGALHALSITASGYDSGQSVTF
                     FQGVSSLGTWRRARRVGSVANITVDHLMASSAIPFIFSAEKINREYFGDGSMRQIAPI
                     SPALHLGAERVLVVGVRQETPSPAVRTEDDEYPSLAQIAGHVLNSIFLDSLEADLERL
                     QRINKTISLISADQLREGGVTLRSVDVLVIAPSADLEKIAARHAHHLPGSIRFLLRGL
                     GAFNRKGSNLVSYLLFEKPFCRELIDLGYKDAMHRKEEILRFLEMPPA"
     gene            4027..4227
                     /locus_tag="AC16FV_02650"
     CDS             4027..4227
                     /locus_tag="AC16FV_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR2848996.1"
                     /translation="MMRILVTLGVILVVVGMAWPWLAKLGLGRLPGDIVIERENYRFY
                     FPITTMILISLVVSLIFWLFRK"
     gene            complement(4425..4805)
                     /locus_tag="AC16FV_02655"
     CDS             complement(4425..4805)
                     /locus_tag="AC16FV_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848997.1"
                     /translation="MRLWVEKAYPYIGGVIAGALLASSLTLGAILTGFLATSKTILIS
                     LHGSGVMEKLKSSTYIKELISYLAEAIWLSFGFSLLVLLGFFLSNSSNYFFDVAWIAA
                     GVMSAFAFIRVTNIILKIIRHNSA"
     gene            complement(4819..>5412)
                     /locus_tag="AC16FV_02660"
     CDS             complement(4819..>5412)
                     /locus_tag="AC16FV_02660"
                     /inference="COORDINATES: protein motif:HMM:NF042819.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6731 family protein"
                     /protein_id="HIR2848998.1"
                     /translation="LYDPKTQHMVIQYNHDGVRSGRIAEYLGQIDDNPDNQYDLEPRF
                     EPAIERKLMRKHIVRSFHFRIATRRLSKADKMAGQALTSTLDLGDHFGAETIDLVFRA
                     SRGKRSSSLKLNEFRHAWRWLRERIGVDEHSVDAVSVFGKDDADSKAEILNLLGHRLK
                     ETFTDLHVDADLRYPRDQRWNALLRARAGWDKLLKSD"
BASE COUNT         1029 a         1609 c         1671 g         1103 t
ORIGIN      
        1 cggccggcgc gtgccgctcg aggattatct cgtcggcgtc gtcaacgatt ggctgcggac
       61 gcgtcgcagt gtttccgtgc agcgccattt cgagggacct cggccgtcgc cggaaatcat
      121 cgcggaacag accctgagtc aggccatcgt gaacatcctg aacaatgccg cggattcttc
      181 gcccgacaac gtcgaggtca gcgcgcgctg gagcgagcag gaactggtgc tggaaatcgg
      241 cgatcgcggc caggggctga cgcccgagac cgcgcgcgcc gtcggccagc cgttcttcac
      301 caccaaggcc ccggggcagg ggctcggcct cgggctgttt ctggcgcatg ccaccctgcg
      361 tcgtttgggc ggatcggtgc agatgtataa tcgcgaaggc ggtggcgtat gcacgcaact
      421 ggttttgccc ctggtcaatc tgcgcgtgga cgggacctca tgaacaccga gaatgcggcg
      481 gacaaaccaa gcttgcttct ggtcgacgat gacgagacgt ttacccgggt gctgtcggcg
      541 gcgctcgaac ggcgcggctt ctcggtgcgt atcgcacgcg atgttgccga ggggttcgcg
      601 gcggcggaga gcgatttgcc cgagtatgcc gtggtcgatc tcaagatgcc cggaccgtct
      661 ggtctggagc tggtcaaaaa actgaaggag ctggatgcgc acacccgcat cgtcgtattg
      721 acgggttacg ccagcgtcgc caccgcggtc gaggccatca agctcggtgc cattcattat
      781 ctcgccaagc cggcggatgc cgatgagatc gtcgccgctt ttcagcgtga cgtcggcaat
      841 acggcggtgg cggtggaaac caaatcgctg ccgctgtcgc gtctcgaatg ggagcacatt
      901 cagaaggtgc tgacggagag tggcggcaac atctccgaaa ccgcgcggcg gctcggcatg
      961 caccggcgca cgctgcaacg caaactcgct aaaagaccgg taaaagcctg attggcagat
     1021 tttgaattcc gttaatcctg tctcttaggg agcctctgat taagtctccc gttcgccctg
     1081 agcttgtcga agggtgaacg ggatataccg taaataacgg atgattcgtc cgttcatggt
     1141 tcgacaggct caccatgaac ggacttgttc agaggttcct tagtttttct cttccagaat
     1201 tttcgccgct tcgtcggcga tctttttccg gctgaacagc aattgccagc ggctcccgcc
     1261 gagctgatgc cagagaaacg ccgagcggat gccggcgaac agcgacgcgc gcaccttggc
     1321 ggcgatcgac gggttgctca ggtggccatg ttcgccgttg accatgatgc gcggtgtgag
     1381 cgtgctgata gactgggtat agagctccgc cagtttttcc accaggcgcg gatgcaccgt
     1441 ttcctcgttc tcggccgtct cgaaaaattt catctgttcg cggatggcct cgatgccgcg
     1501 gcggacggcg tcctgcattt ccgggcgccg gcgcaactgg ccttcgaggt gaatcatggc
     1561 gatgacatag cgggcgatct cgacgtcgct ggcgtcggtg ttaccgccgg ccagcttgtt
     1621 gcgcagcagc tcaagcccgg gcttgatgcc ctgcgcccca ccgtagacgg attcggtagt
     1681 gggcgcatcg atgatcagca ggctctgaac gctggcagcc agggcgggtg cctcggcgcg
     1741 gccttcacgc gcgagttgct gcgccagccg cgcggcctgg aacaggcccg cgagggccac
     1801 tatgcggtcg gtgtaggccg tactcatctg tgtgcggtct gaaacagtcg atggggcgac
     1861 aagtttagac ggaagcggcg ggagcggcca gaaacgggat cgaccaaggc cgcggcgcgg
     1921 tggggatgac ggggtcagtc ttccttcatg caggcgcgtt cgacctcgac gcgcttcacg
     1981 ccttgcacgc cttcgacggc gcgcttgatt tccgccacga tgttctcggc gaagggacaa
     2041 tagggcgtgg tcaactggat gtcgagatgg acgtagtcat cgacgatgtc catttcctgg
     2101 atgaaaccga gatcgacgac gttgtgcttc agctccgggt cgatgacctt cctgagcgcg
     2161 tcgtaaacca tggcttcggt gattcgtgcg accatcggaa caacctctgt atcaatataa
     2221 tgaacccagt ctatcaaatc atgtcgtgac tgtaccgcat acccgatggc catgatcaac
     2281 ttaagggaaa ttctcccgat cccgggtcgg gttacagtcg cagcggcttc accggcgctt
     2341 ccatccattc atgcgcatga cgccagtcaa cgccgggatc gtccacgaag agttcgattt
     2401 cattgccgtc cggatcgcgc aggtacaggc tctggctcac ggtgtgatcg gacatgccct
     2461 cgatgtcgtg gccatgggcg tgcacgcggt cgcgcgcccg gcgcaggtcg tcgaggttgt
     2521 cgccgacctt ccagcccacg tgatacaggc cgatgcgccg gccttgcaac ggcccgggcg
     2581 cggcgccgat ttcgatcagc aacagctcgt gatgcgtgct gccgccggtg agcatggcgg
     2641 cgcggccgtt gaaaatccgg ccggcgagtt ccaaccccac gacgtcccgg tagaacgccg
     2701 tggatttttc cagatcgcgt acgtagaaaa ccacatgccc gagagtaacc atggttttat
     2761 ccttcgttga ctctaatagg gagcgacgcg ggccatcata aaccaacccg tgaaatcccc
     2821 gcgtcggtca tcgtcacgct tggcgcaaca gggactccgt gaaagcggaa acatgaagga
     2881 tatggcggat aaacccaggg tagggctgat actggccggt ggcggcgcgc gcgcggccta
     2941 ccaggtgggc gtgctgaagg ccattgccga gatgctcccg cgcgattgcc ccaatccgtt
     3001 tcccattctc tgcggcacgt cggccggcgc catcaacgcc accgcgctgg cgatttacgc
     3061 cacccgtttc catgaagggg tgcggcgcct cgcctacgta tggcgcaatt tcaacgtgcg
     3121 ccaggtattc cgcgccgaca cgctcggcgt cctcgcgaat gggatgcgct ggtttctggc
     3181 cacgatgctt ggtggattgg ggcgacgtaa cccgcgggcg ctgctcgatc gcgcgccgct
     3241 gcggcgcctg ctgcaacaga ccatgccctg cgacagaatc caggcgtcga tcgacgccgg
     3301 cgcgttgcat gcgctgagca tcacggcctc gggttacgat tcggggcaat cggtgacatt
     3361 ttttcagggc gtgtcttcat tgggcacgtg gcggcgcgcg cggcgtgtcg gcagcgtggc
     3421 caacatcacc gtcgatcatc tcatggcgtc ctcggccatt cccttcatct tctccgcgga
     3481 gaaaatcaac cgcgaatatt tcggcgacgg ttccatgcgc cagatcgcgc ccatcagtcc
     3541 cgcgctgcac ctgggcgcgg agcgcgtgct ggtggtcggc gtgcgccagg aaactccgtc
     3601 cccggcggtg cgcacggagg acgacgaata tccctcgctg gcgcagattg ccggccatgt
     3661 cctgaacagc atattcctgg acagcctcga ggccgatctc gaacgcctgc aacgcattaa
     3721 caaaaccatt agcctgattt ccgccgatca actgcgcgag ggcggggtga cgctgcgcag
     3781 cgtggacgtg ctggtgatcg cgccgagcgc ggacctggaa aagatcgccg cgcgccacgc
     3841 gcaccacctg ccgggctcga tacgcttcct gctgcgcggg ctcggcgcct tcaaccgcaa
     3901 gggctcgaat ctggtgagtt acctgttgtt cgagaaaccc ttctgccgcg agctgatcga
     3961 tctgggctac aaggatgcaa tgcaccgcaa ggaggagatc ctgcggttcc tggagatgcc
     4021 gcccgcatga tgcgcatcct cgtcacgctc ggcgtcattc tcgtcgttgt ggggatggct
     4081 tggccgtggc tcgccaagct cgggctcggg cgcctgccgg gggatatcgt gatcgagcgg
     4141 gagaattacc ggttttattt tccgatcacg acgatgattt tgataagttt ggtggtgtcg
     4201 ttgattttct ggttgtttcg gaaataggca gttcgtgcgc gcagcgcgca cgaactgtcg
     4261 tcaccccggc gaaggccatt cgctcgtcat tccggcggaa gccggaatct aggatttaaa
     4321 cggctcgcgc gctttgcgcg cgactcaaaa aagcatactt gtggatggct gacccacggc
     4381 agaactcgcc ggtagacttc ttgacgggat ttacgcgtgg cgcgctatgc gctgttgtgt
     4441 cggatgattt ttaggatgat gttggtgacg cggataaacg caaatgccga catcacgccg
     4501 gccgcgatcc acgccacgtc gaagaaatag ttcgacgagt tggatagaaa aaaacccagc
     4561 aacaccaaca gactgaaacc gaacgacagc cagattgcct cagccagata gctgataagt
     4621 tccttaatgt aagtgctcga ctttagtttc tccatcactc ccgagccatg aagactgatc
     4681 aggatagttt tggacgtcgc gaggaaacca gtcaatattg cgcccaaggt gagcgatgac
     4741 gcgagcagcg cgcccgcgat cacgccgccg atgtacggat aggccttttc aacccacaag
     4801 cgcatgcgcc cagtatcctc aatccgactt cagtagttta tcccagcctg ctcgcgcacg
     4861 aagaagcgca ttccaacgtt ggtcgcgagg ataacgcaaa tcggcatcga catggagatc
     4921 cgtgaacgtc tcctttaggc gatgaccgag cagattcaga atctcggcct tcgagtccgc
     4981 gtcatccttg ccgaaaaccg ataccgcatc cacagagtgc tcgtcaacgc ctattcgctc
     5041 tcgcaaccag cgccaagcgt gcctgaactc attgagtttc aggctgctcg aacgcttgcc
     5101 gcgcgacgcg cgaaatacca agtctatggt ctcggcgccg aaatgatcgc caagatcgag
     5161 cgtgctcgtc aatgcttggc ccgccatttt gtctgcttta gaaagacggc gtgttgcgat
     5221 gcggaagtga aacgagcgca ctatatgctt gcgcatcagc ttgcgctcga tcgccggctc
     5281 gaatcgcggc tcaaggtcat actggttgtc agggttatca tcgatctggc ccagatattc
     5341 ggcgatccgg cccgagcgga caccgtcgtg gttgtactgg atcaccatgt gctgcgtctt
     5401 cggatcgtaa ag
//
LOCUS       DBNGZE010000105         5067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1890319, whole genome shotgun sequence.
ACCESSION   DBNGZE010000105 DBNGZE010000000
VERSION     DBNGZE010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1890319"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..830
                     /locus_tag="AC16FV_02665"
     CDS             246..830
                     /locus_tag="AC16FV_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2848999.1"
                     /translation="MKKALTLLNTLIIGCAAIVWTGTGESSDPVLADREMPADIVSTC
                     ALTQAGEIGACDHPAFSRRWVAKTVRGDLFIVTNERCTGSECRAWLVEKTEAAATTLL
                     SFDATYRLREDMNGYPVIESYTGMSATEGAYSRYEWNGNAYTRTASRLVYNVDGAECG
                     TREECRHAANEAIAQKQVDRAVRIWENVGGVSWI"
     gene            996..2018
                     /locus_tag="AC16FV_02670"
     CDS             996..2018
                     /locus_tag="AC16FV_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HIR2849000.1"
                     /translation="MTFKIRNQSSGREFSVEENETVLAAALRQGVMLPYSCRNGACGT
                     CKGKVLAGQVDYGTYETKAMSETERQQGYALFCQTKPLSDLVIEAKEIAAARDIPIRI
                     MPARVVKMEKLADDVMRLSLKLAEGQRLQYLAGQYVDILLSGNKRRSFSLSTSPLSDE
                     LLQLHIRHVPGGFFTGHVFTKMQEKDLLRFQGPFGMFFLREESDHPAILVAGGTGFAP
                     IKGILEYAFAKGIARPLHLYWGVRAKRDLYLPDLPQAWAREHGNFRFTPVLSEPRPED
                     HWDGRQGWVHEAVAADYPDLSRHEVYASGPPPMIDAIKDMVKKHGLPEDRLYYDSFEF
                     AHASAP"
     gene            2029..2244
                     /locus_tag="AC16FV_02675"
     CDS             2029..2244
                     /locus_tag="AC16FV_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849001.1"
                     /translation="MRMILTVMFLAVTLSACGEKKEVKDTVFGSQVQMKDKARAVEDK
                     LREGAEKNREALKASEQDAGAEQQKGY"
     gene            2256..2651
                     /locus_tag="AC16FV_02680"
     CDS             2256..2651
                     /locus_tag="AC16FV_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849002.1"
                     /translation="MIIQLHDKPVRVELSAAAERALAKRAGPLYAEVQLIFGCMIAKR
                     VWFRDEAVENAVPVNSKLSVWFRPARYQKACSFDDIDNGAEASDYPVVGDRGRFVPDL
                     LSIDYRGGKWVGDFTYSMDVFRAQHPLPN"
     gene            complement(2678..3922)
                     /locus_tag="AC16FV_02685"
     CDS             complement(2678..3922)
                     /locus_tag="AC16FV_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR2849003.1"
                     /translation="MKTLIFIIVLLFAATLITFYALENPGYVLISRAPWSIEMTLTIF
                     IPLMIVAFFLFYLLLFMIVRLWRIPRDVGRWRAKRHSREARDALIQGLTNLAEGNWVE
                     AETQLLAGMRHGDTPLLNYLGAAMACEGQGNSEKRDEYLALAHKNAPQHDLAIGMTQA
                     YLQHQARQMEQSLATLNELRAETPKHQQVLKLLAQVHLELRDWTGLINLIPDLRQSYA
                     MTTKEIDELELQAHRELLRLSIPSGRQGVLEKAWNAVPKSLRRHPSMIAIYARQLIQQ
                     NEMTQAESALRAAIEDNWDSTLVELYGQAQGPNPADQLETAEGWLTPHRDDPKLLLTL
                     ARLAARSNEDSKARGYYETCLTQHGPADAYRELGNLLEKLGEKDKALNTYRRGLDMVA
                     TELRATPTRGLGSAISRFRAAR"
     gene            complement(3919..4998)
                     /locus_tag="AC16FV_02690"
     CDS             complement(3919..4998)
                     /locus_tag="AC16FV_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR2849004.1"
                     /translation="MTDTDAKNPPAEPAAASRRAKTAPKKSENESGHGRVRGGLAVIL
                     ATLALIASGYLWYVMFYESADLFSRDIVGALDRIESDYNQTLETSANTEKDIKTLKEN
                     QDAIKASLEKINSDLRRNRLEWALTETEQLMIIANNRLQLARDSHSALAALRAADRQL
                     EQLAMPKFLPVRRELAREISLLESHEKLDVSGITLRLATLADNVDQLPLALDIRPREI
                     GETKTPVKTGDTQVAAADGNWRQTARSLWQDVLSLVRIRNDMASQRPLLPPEQQYFLR
                     ENLRLMLNSAQQALLQGSVPTYQQNLKTAQRLLKDYFDVESQVVTAMQTELTRLQTMK
                     ILTDMPDISASLTTLRRLSGRGVEP"
BASE COUNT          927 a         1487 c         1631 g         1022 t
ORIGIN      
        1 ttcaagcatg ggccgcattt aagggagcag gggcgggaat gtcaaatacg caatatttga
       61 ctttgcgccc ggttttgccg cagagtgacg cggtccgtaa cggtggtttc gcttacggcc
      121 cacggccggc ctgggagtgg cccgccgtcc cccgatgtaa tccgctgtaa ataatggatt
      181 tttccaaggc tggacccact ggcacggaat atgcctctca taccggtaag agaaggtaat
      241 gatccatgaa aaaggccctg acactgttga atacgctgat catcggttgc gccgccatcg
      301 tctggaccgg caccggtgaa agttccgacc cggtgctcgc cgaccgcgag atgccggccg
      361 atattgtcag tacctgcgca ctgacccagg ctggcgaaat cggcgcctgc gatcatccgg
      421 ccttcagccg ccgctgggtg gccaagaccg tccggggcga cctcttcatc gtcaccaacg
      481 aacgttgcac gggcagcgaa tgccgcgcct ggctggtgga gaagaccgaa gccgccgcga
      541 ccacgctgct cagttttgac gccacctacc gcctgcgcga ggacatgaac ggttacccgg
      601 tgatcgagag ttataccgga atgtccgcca ccgagggcgc ctacagccgc tatgagtgga
      661 acggcaacgc ctacacccgc accgccagtc gtctggtgta taacgtggat ggcgcggaat
      721 gcggcacgcg tgaggaatgc cgccacgcgg ccaacgaagc gatcgcgcag aaacaggtcg
      781 atcgcgctgt gcgcatctgg gaaaacgtcg gcggcgtctc ctggatctga tccgcactcc
      841 agactttcct cccttccgac tcatcaccat gcccgcgtaa gcgggcatgt gctttcaact
      901 gtgccaaacc cggttttaat ccgtggtttt tctcctcgcc gttttgggga acaatagggt
      961 ttcgcagcga ataaaaacca tcgagccccg ttcccatgac cttcaagata cgcaaccaat
     1021 ccagtggccg tgaattcagc gtggaggaaa atgaaaccgt gctggcggcg gctctgcgtc
     1081 agggtgtgat gctgccctat agctgccgca acggcgcctg cggtacctgc aagggaaaag
     1141 ttctcgcagg ccaggtcgat tacggaactt acgagaccaa ggccatgagc gaaacggaaa
     1201 ggcagcaggg ctacgcgctt ttctgtcaga cgaagccgtt atctgacttg gtgattgaag
     1261 cgaaggaaat cgccgcggcc agggatatcc ctattcgcat catgccggcg cgcgtggtca
     1321 aaatggaaaa gctcgcggac gacgtgatgc gactctcgct caagctcgcc gagggccagc
     1381 gcctgcaata tctggccggg cagtacgtcg atatactttt gagcggcaac aagcggcgca
     1441 gcttttcgct ttcgacctcg ccgctctcgg atgaattgct gcaactgcac atccgccatg
     1501 tgccgggcgg cttcttcacc ggtcacgtgt tcaccaagat gcaggaaaaa gacctgctgc
     1561 gttttcaggg tccgttcggc atgtttttcc tgcgcgaaga atctgatcat ccggcgatcc
     1621 tggtcgcggg cggcaccggc ttcgcgccca tcaagggcat cctggaatat gctttcgcca
     1681 agggcatcgc gcgcccactg cacctgtact ggggcgtgcg cgccaagcgc gatctgtatc
     1741 tgcccgatct gccgcaggcc tgggccaggg aacacggcaa tttcagattc acgccggtgt
     1801 tgtccgagcc gcggccggag gatcactggg acgggcgcca ggggtgggtg cacgaggcgg
     1861 tggccgcgga ttacccggat ttgagccggc acgaggtgta tgccagcggc ccgccaccca
     1921 tgatcgacgc gatcaaggac atggtgaaaa aacatggtct gcccgaggac cgcctgtatt
     1981 atgattcctt tgagttcgcc cacgcgtcgg ccccgtaggg aggaaaccat gcgcatgatt
     2041 ctgacggtca tgttcctcgc tgtgacgttg tccgcctgcg gcgagaagaa agaagtcaaa
     2101 gacacggtgt tcggttctca agtgcagatg aaggacaagg cccgcgcggt ggaagacaaa
     2161 ctcagggaag gcgcggagaa aaaccgcgaa gcgctgaagg catccgagca ggatgccggc
     2221 gcggaacaac aaaaaggtta ttgaggcatt tcaaaatgat catccagttg catgacaagc
     2281 cggtgcgcgt ggaactgagc gcggccgccg agcgtgcact ggcgaagcgc gccgggccgc
     2341 tgtatgccga ggtgcagctg atcttcggct gcatgatcgc caaacgcgtg tggtttcggg
     2401 atgaagcggt tgagaacgcg gttccggtga actcgaaact gagtgtgtgg ttccggccgg
     2461 cgcgctacca gaaggcgtgc agtttcgatg acatcgataa cggcgccgag gccagcgact
     2521 acccggtggt cggcgaccgt ggccgtttcg tgccggacct gctttccatc gattaccgcg
     2581 gcggaaaatg ggttggcgat tttacctatt cgatggatgt gttccgggcg caacatccat
     2641 tgccgaatta aatcggcgga aagcaagaac ccgtggatca gcgcgccgcg cggaaacggg
     2701 agatggctga acccagtccg cgcgtagggg tggcgcggag ttcggtggcg accatatcga
     2761 ggccgcggcg gtaggtgttg agcgccttgt ccttctcgcc gagtttttcc agtaaattgc
     2821 ccagttcgcg gtaagcatcc gccgggccgt gctgcgtcag gcaggtttca taatagccgc
     2881 gcgccttgct gtcctcgttg ctgcgcgccg ccaggcgcgc cagtgtcagc agcaatttcg
     2941 gatcgtcacg atgcggagtc agccagccct cagcggtttc cagctgatcc gccggattcg
     3001 gtccctgcgc ctggccgtac agctccacca gcgtcgagtc ccagttgtcc tcgatggccg
     3061 cgcgcagcgc cgattcggcc tgtgtcatct cgttctgctg gatcaactgg cgcgcataga
     3121 tggcgatcat cgagggatga cggcgcaagg atttcggcac cgcgttccag gccttttcca
     3181 gcacgccctg ccggccggag gggatggaca gccgcagcag ttcacggtgc gcctgcaatt
     3241 ccagctcgtc gatttccttc gtggtcatgg cgtagctctg gcgcaggtcc gggatcagat
     3301 tgatgaggcc ggtccagtcg cgcaattcga gatgcacctg cgccagcagt ttcagaacct
     3361 gctgatgttt cggggtctcg gcgcgcagtt cgttcagggt tgccagtgac tgttccatct
     3421 ggcgcgcctg atgctgcaaa taggcctgcg tcatgccgat ggcgaggtcg tgttgcggcg
     3481 cgttcttgtg cgccagcgcc aggtattcgt cgcgtttttc gctgttgccc tggccctcgc
     3541 aggccatggc cgcgcccagg taattcagca gcggcgtgtc gccgtggcgc atgccggcga
     3601 gcagctgcgt ttccgcctcg acccagttgc cttccgccag attcgtcagc ccctggatga
     3661 gcgcgtcgcg cgcctcgcgg ctgtgccgtt tcgcgcgcca gcggccgacg tcgcgcggga
     3721 tgcgccacag gcgcacgatc atgaacagca gcaggtagaa caggaaaaac gccacgatca
     3781 tcagcgggat gaagatcgtc agcgtcatct cgatgctcca cggggcgcgc gaaatcagca
     3841 cgtagcccgg attctcgagg gcgtagaagg tgatcagcgt cgcggcgaac agcagaacga
     3901 tgatgaaaat cagcgtcttc acggttccac gccccggccg ctcaggcgcc gcagagtggt
     3961 cagcgacgcg ctgatgtccg gcatgtcggt gaggatcttc atggtttgca ggcgggtcag
     4021 ttcggtctgc atcgcggtga ccacctgcga ttcgacgtca aaataatcct tgagcaggcg
     4081 ctgcgccgtc ttgagattct gctgataggt gggaacgctg ccctgcaaca gtgcctgttg
     4141 cgcgctgttg agcatcaggc gcaggttctc gcgcaggaag tactgttgtt ccggcggcag
     4201 cagggggcgc tgcgaggcca tgtcattgcg gatgcgcacc agtgaaagaa cgtcctgcca
     4261 cagactgcgc gcggtctggc gccagttgcc gtccgcggcg gcaacctgtg tgtcgcccgt
     4321 ttttaccggt gtcttggtct ccccgatttc acgcggccgg atgtccagcg ccagcggcaa
     4381 ctggtccacg ttgtcggcca gggttgcgag acgcagggtg atgccgctga cgtccagttt
     4441 ttcgtgggat tccagcaggg aaatctcgcg cgcgagttcg cgccgcaccg gcaggaactt
     4501 cggcatcgcc agttgctcca gctgccggtc ggcggcgcgc agggcggcca gggcggagtg
     4561 ggagtcacgc gccagttgca ggcggttgtt ggcgatgatc atcagctgtt cggtttccgt
     4621 cagcgcccat tcgagccggt tgcgtcgcag gtcgctgttg attttttcca gcgaggcctt
     4681 gatcgcgtcc tggttttcct tgagggtctt gatgtccttt tcggtgttgg cgctggtttc
     4741 cagcgtctgg ttgtaatcgg actcgatgcg atcgagcgcg ccgacgatat cgcgcgagaa
     4801 caggtcggcg ctctcgtaga acatgacgta ccacaggtag ccgctggcga tgagcgcgag
     4861 cgtggcgaga atgaccgcga gtccaccgcg cacccgcccg tggcctgatt cattctctga
     4921 tttctttggc gcggttttgg cgcgccgaga ggcggcggcc ggttcggcag ggggattttt
     4981 ggcgtccgtg tcggtcatgg gatttccggc ctgtgtgcga agcaggatta taaggtgttt
     5041 tgcccgtccc gccacgcctg gacggcc
//
LOCUS       DBNGZE010000106         2728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_153758, whole genome shotgun sequence.
ACCESSION   DBNGZE010000106 DBNGZE010000000
VERSION     DBNGZE010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_153758"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="AC16FV_02695"
     CDS             <1..318
                     /locus_tag="AC16FV_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HIR2849005.1"
                     /translation="FSSHWLGGVHYRYYNQTAASFYGDNFLTPMNYMARDKELSTFKS
                     HVVGGKVSYTFRNHPSFVNKSTINIAYDQMRFEYDDFTDVRTQAPYAFNAGVVQLYLS
                     VWF"
     gene            354..923
                     /locus_tag="AC16FV_02700"
     CDS             354..923
                     /locus_tag="AC16FV_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR2849006.1"
                     /translation="MNDRNFLKQPGLFRTALAFFAIALAVPLTALAAETAPPAGKLKS
                     LDQQAQSLKQEVMELNRDLLALEEELLFPASTQVAVFLSMDIGSLFDLDAVEVRLDDK
                     VVAHYLYTEREIEALRRGGVHRLYLGNLRTGKHELAAFFTGKGPHNRDYRRGAALEFD
                     KPTGPKYIELQIKDVARNLQPEFRVKEWQ"
     gene            948..2528
                     /locus_tag="AC16FV_02705"
     CDS             948..2528
                     /locus_tag="AC16FV_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849007.1"
                     /translation="MNKRRFAAFVAALCLCACAVTSAHGSARRGESREIKDPHYGEVL
                     FYFYQQQYFSAITNLMTARGFQRVPHHDDEAELLLGGMYLSYGLHGQAGEIFQRLIDA
                     GAPPPVRDRAWFYLAKIRYQRGYRDEAEQAVSRIRDALPGELEEERQLLQAQLLMERQ
                     EYREAARVLARMRGESVWAQYGRYNLGVALIKAGEGDAGAALLEEVGRKAATGEELKS
                     LKDKANVALGYTYLRDGAPARAQARLEQVRLDGLLSNKALLGMGWAFSAQDQHERSLI
                     PWTELYKRNVIDATVQEALLAVPYALGRAGAYQQSLQHYENAIGVYTKEMTRIDDSIA
                     AIRSGRMIGGILQQDAENEMGWFWRMHQIPDAPESRYLAHLMATHDFQEMLKNYRDLR
                     FLTLNLDEWAGKIGVYDDMLATRRLAYAQRLPKVLQSERSRDFAGLSARRDRLAAELA
                     RAENDNDVMALANDRERQLLARLERVEQILKRLEGREDMAAARDKHRLLRGLLYADVA
                     SDYSPRLWQARKNLKELD"
BASE COUNT          543 a          777 c          879 g          529 t
ORIGIN      
        1 ttcagcagcc attggctcgg gggcgtgcat taccggtatt acaaccagac ggcggcatct
       61 ttttatggag acaatttcct gaccccgatg aattacatgg cgcgtgacaa ggagctgagc
      121 acgttcaaga gccacgttgt cggcggcaag gtgagctata cgttccggaa tcatccctca
      181 tttgtgaaca agtcgacgat caatattgcc tacgaccaga tgaggttcga gtacgacgat
      241 ttcaccgatg tccggaccca ggcgccttat gcgttcaatg ccggggtggt tcagctgtat
      301 ttatcggtat ggttttaaac gcctgtgtcc atgacatgtg agtgagccgg catatgaatg
      361 atagaaattt tctgaagcaa cccgggctat ttcgcacggc ccttgcgttt tttgcgatcg
      421 cgctggcggt gccgctgacg gcgctggcgg ccgaaactgc gccgcccgcc ggcaaactca
      481 agagcctcga ccagcaggcg caatcgctga aacaggaggt gatggaactg aatcgcgatc
      541 tgctggcact ggaggaggag ctgcttttcc ccgccagcac gcaggtggcg gtgttcctgt
      601 ccatggacat cggcagcctg ttcgacctcg atgctgtcga ggtcaggctg gatgacaagg
      661 tggtggcgca ttatctctac accgagcgcg agatcgaagc actgcggcgc ggcggcgtgc
      721 atcgccttta cctcggcaat ttgcgcacgg gcaagcacga actggcagcg tttttcaccg
      781 gcaagggtcc gcacaaccgc gattaccggc gcggcgccgc gcttgaattc gacaaaccga
      841 ccggaccgaa atacatcgag ctccagataa aagacgtggc gcgcaacctg cagccggaat
      901 ttcgcgtcaa ggaatggcag taacgtgtca tgtccggcgc cgcgctcgtg aataaaagac
      961 gtttcgccgc atttgtcgcg gcgttgtgtc tgtgcgcctg cgcggtgacg agcgcccacg
     1021 gcagcgcgcg gcgcggcgaa tcccgcgaaa tcaaggaccc gcattatggc gaggtgctgt
     1081 tttatttcta tcaacaacaa tatttttccg ctatcacgaa tctgatgacg gcgcgggggt
     1141 tccagcgtgt tcctcatcat gacgatgaag ccgagttgtt gctgggggga atgtatcttt
     1201 cctacggcct gcacggacag gccggagaaa tatttcagcg tttgattgat gccggcgccc
     1261 cgccgccggt tcgtgaccgc gcctggtttt atttggccaa gatccgctat caacgcggct
     1321 atcgggacga ggccgagcag gcggtatccc gcattcgcga tgcactgccg ggagagctgg
     1381 aggaagaacg tcagctgttg caggcgcagc tgctgatgga acggcaggaa tatcgcgagg
     1441 ccgcgcgggt gctggcgcgc atgcgcggcg aatcggtctg ggcacagtac ggccgataca
     1501 acctcggtgt ggccctgatc aaggccgggg agggcgatgc cggcgccgcg ctgctggagg
     1561 aggtcggtcg caaagcggca acgggagagg aactgaaatc cctcaaggac aaggccaacg
     1621 tggcgctggg ttatacctat ctgcgcgatg gcgcgccggc gcgcgcccag gcccggcttg
     1681 agcaggtgcg ccttgacggg ttgctgtcga acaaggccct gctcggcatg ggctgggcct
     1741 tctccgccca ggaccagcat gaacgatccc tgattccctg gaccgagctg tataagcgca
     1801 acgtcatcga cgccacggta caggaggcat tgctcgcggt gccctacgcc ctgggtcgcg
     1861 ccggcgccta tcagcaatca ctgcagcatt acgagaacgc catcggtgtg tacaccaagg
     1921 agatgacgcg tatcgatgat tcgatagcgg ccatccgctc gggaagaatg atcggcggta
     1981 tcttgcagca agatgccgaa aacgagatgg gatggttctg gcgcatgcac cagatcccgg
     2041 acgcgccgga atcgcgctat ctggcgcacc tcatggccac ccatgatttc caggaaatgc
     2101 tcaagaatta ccgcgacctg cgttttttga cgctcaatct cgatgaatgg gccggcaaga
     2161 tcggcgtcta tgacgacatg ctcgccacgc ggcggctggc ttatgcgcaa cggctgccga
     2221 aagtcctgca gagcgagcgt tcgcgggatt tcgccgggct gtcggcgcgg cgcgatcgcc
     2281 tggcggccga actcgcgcgc gccgagaatg acaacgacgt gatggcgctg gcaaatgacc
     2341 gggagcggca actgctggcg cgcctggaac gtgtggagca aatcctcaag cgcctggaag
     2401 gacgcgaaga catggccgcc gcgcgcgaca aacatcgtct gctgcgcggc ctgctatacg
     2461 ccgacgttgc ctccgattat tcgccgcgcc tgtggcaggc gaggaaaaac ctgaaagaac
     2521 tggattagcg tctggcggag accggtgcgc ggcgcgaggc gttgctgcgc gcgcaggtgg
     2581 aggcgccgcg ggcgtttgat gattttgccg cgcgcattgt ccggttgcgt gcgcggattt
     2641 cgcaattgca ggaccggatc cggacggtgt catccctgca tgcgcgttat ctcgaggatc
     2701 agtccgttgc cgaacttgcg gcccagaa
//
LOCUS       DBNGZE010000107         1744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_779175, whole genome shotgun sequence.
ACCESSION   DBNGZE010000107 DBNGZE010000000
VERSION     DBNGZE010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_779175"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="AC16FV_02710"
     CDS             <1..422
                     /locus_tag="AC16FV_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2849008.1"
                     /translation="AHIHEFVESLPDKYDTIVGERGLKLSGGEKQRVAIARTLLKNPP
                     ILVFDEATSALDSRTEKAIQAELQEIATNHTTLVIAHRLSTIIDADPILVMEHGRIIE
                     RGAHGELLAQNGAYAHMWALQQQEQKEEEEKLRAVSN"
     gene            559..1635
                     /locus_tag="AC16FV_02715"
     CDS             559..1635
                     /locus_tag="AC16FV_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2849009.1"
                     /translation="MRPALILAILEREFLSTRKGHHTPVMLWGPPGVGKSQMVAQVAR
                     KYGVPVIDIRLSQMEPTDLRGIPFRVGDVVEWAIPAMLPDAQRHGTEGILFLDEITSA
                     APTVSAAAYQLILDRRLGAYQVPEHWAIFASGNRQGDRGVTYSMPAPLANRFSHFGVE
                     THLDDWVAWAYANHIDERVIAFLRFRPELLFDFDPAHNPVAFPSPRSWEFAHRALAKF
                     GETPDLLLGSLEACVGPAAGVELKAFIDNLDNLPDIDAIINGMSTTVPKETDLQYAVA
                     AALVGRAIHAKGGVDEAKVFGNILDYAGRFPSREMGVMLVSDMQRAVGEKLFQLPQFA
                     QWAKKIANVMIFDMWQKPAAAKAK"
BASE COUNT          376 a          552 c          511 g          305 t
ORIGIN      
        1 cggcgcatat tcatgagttc gtggaatcgc tgccggacaa gtacgacacc atcgtgggcg
       61 agcgcggcct caagctgtcc gggggcgaaa aacagcgtgt cgccatcgcg cgcacgctgc
      121 tgaaaaaccc gccgatcctg gtgttcgacg aggccacctc ggcgctcgat tcgcgcaccg
      181 agaaggcgat tcaggcggaa ttgcaggaga tcgcgaccaa tcacacgacc ctggtcatcg
      241 cccatcgcct gtccaccatc attgacgccg acccgattct ggtgatggag cacggccgca
      301 tcatcgagcg cggcgcccac ggcgagctgc tggcacagaa cggcgcctac gcccacatgt
      361 gggcgctgca acagcaggaa cagaaggaag aggaagaaaa gctgagagcg gtcagcaact
      421 gaaaagttgc tacaacgacg taccgaccgc ccttctttgc gaagcgctcc tcgagggcct
      481 gaagatggac gctcggcaac ggcaaaatta tactctgacg atcaaccgta ttttaaaagt
      541 acagcaaccg gagatttcat gcgcccagcg cttattcttg ctattctcga acgcgaattc
      601 ctgagcaccc gcaagggcca ccatacgccg gtcatgctgt ggggcccgcc cggcgtcggc
      661 aaatcgcaaa tggtggcgca ggtggcgaga aaatacggcg tgccggtcat cgacatccgc
      721 ctgtcacaga tggaaccgac cgacctgcgc ggcattccgt tccgcgtcgg cgacgtcgtc
      781 gagtgggcca tcccggcgat gctgccggat gcccagcgcc acggcaccga gggcattctg
      841 tttctcgatg aaatcacctc ggcggcgccg acggtctctg ccgccgccta ccaattgatc
      901 ctcgaccgcc gtctcggcgc ctatcaggtg ccggagcact gggccatctt cgcctccggc
      961 aaccgccagg gcgaccgcgg cgttacttac agcatgccgg cgccgctggc caaccgcttc
     1021 tcgcacttcg gggtcgagac ccatctcgat gactgggtgg cgtgggccta tgcgaaccat
     1081 atcgacgagc gcgtcatcgc gttcctgcgt ttccgccctg agttgttgtt cgacttcgat
     1141 ccggcgcaca atcccgtggc ctttccgagc ccgcgctcgt gggaattcgc gcaccgcgcg
     1201 ctcgccaagt tcggcgaaac accggacctg ctgctgggtt cgctcgaggc ctgtgttggc
     1261 cccgccgccg gcgtggaact caaggccttc atcgacaacc tcgacaacct gccggacatc
     1321 gacgccatca tcaacggcat gtccaccacg gtaccgaagg aaaccgacct gcaatacgcc
     1381 gtggccgcgg cgctcgtggg ccgcgcgatc cacgccaagg gcggcgtcga cgaggccaag
     1441 gtgttcggca atatcctcga ctacgccggg cgtttcccga gccgcgaaat gggcgtaatg
     1501 ctggtctcgg acatgcagcg cgccgtgggc gaaaaactct tccaattgcc gcaattcgca
     1561 caatgggcca agaagatcgc caacgtcatg atcttcgaca tgtggcaaaa acccgccgcc
     1621 gctaaagcaa aataataaac aggattaaca ggatttacag gattaagttg aaaattgggg
     1681 gatgagaaaa gaaatgctgg aggacaagga tcttactgaa aagatcatcg cgtgtgcctt
     1741 caga
//
LOCUS       DBNGZE010000108         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1054655, whole genome shotgun sequence.
ACCESSION   DBNGZE010000108 DBNGZE010000000
VERSION     DBNGZE010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1054655"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1172)
                     /locus_tag="AC16FV_02720"
     CDS             complement(279..1172)
                     /locus_tag="AC16FV_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008389224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR2849010.1"
                     /translation="MDWDKLKVFHAAAEAGSFTHAGERLGLSQSAVSRQVSALEAELS
                     VSLFHRHARGLILTEQGELLYRTAHEVFMKLEAARTKLTDSRERPNGELKVSTTPGIG
                     VHWLTPRLGEFLDLYPDIQITLITTDEELDLAMREADVAIRLRQPTQPDLIQRKLFSV
                     HFHAYASPDYLKRFGTPRTHEELDKHRIILLGGGVVPAHLQNRRWLLEIGRDGKGPRS
                     PHLTVNNVLGVLRACQRGLGIAVLPDYLVEENGGLVQLFGDADTLALDAFFVYPEELK
                     SVARIQVFRDFLVANAQRWHF"
     gene            complement(1194..>1707)
                     /locus_tag="AC16FV_02725"
     CDS             complement(1194..>1707)
                     /locus_tag="AC16FV_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR2849011.1"
                     /translation="VVGGGNTAVEEALFLTNFASKVTVVHRREAFRAEKILQDRLFKH
                     PKIEVIWDSVLEDITGGDNPLKVSGVRLKNVKTSAVTERRVDGVFIAIGHSPASELFA
                     GQVQMKPSGYIITAGHSTVTSVPGVFAAGDVTDDIYRQAVTAAGQGCMAALEAEKFLA
                     AHEHQRAAAE"
BASE COUNT          344 a          500 c          522 g          341 t
ORIGIN      
        1 aaaaaaccgg gccccatttg gggcccggct tcagtgattg ccggcgtggg ggccggcaac
       61 tcttccagag gggaacatgc tgctcgccag cgattctggg aggattcacc cggcgcaaca
      121 gcgagcccgc agtatgcgaa gcatgcatca gcacgacaac gctaacagcc gcatgactgc
      181 catgcgcaat cgcaatttgt tgggatttcg tcgatgggcc gacaccggcg tccgtgccga
      241 atcggcgatt ttttgccgcg ggtttatggc aaaacgaatc agaaatgcca gcgctgcgcg
      301 ttggctacca ggaagtcgcg aaagacctgg atgcgcgcga ccgatttgag ttcctcagga
      361 taaacaaaaa aggcatcgag cgcgagcgtg tcggcatccc cgaacaactg caccagcccg
      421 ccgttttcct cgaccagata atcgggcagc acggcgattc cgagcccgcg ttggcacgcg
      481 cgcagaacgc caagcacgtt gttcactgtc agatgcggcg agcgcggacc tttgccgtcg
      541 cgacctattt ccagaagcca gcgccgattc tgcagatgcg ccggcactac gccaccacca
      601 agaagaatga tgcgatgctt gtcgagctct tcgtgtgtcc gcggcgtgcc gaaccgcttg
      661 aggtagtcag gtgaggcgta ggcgtggaaa tgcacggaaa acagtttgcg ctggatcaga
      721 tcgggctggg tcggctgccg cagtcggata gcgacatcgg cttcgcgcat cgccaggtcc
      781 agctcctcgt cagtggtaat gagcgtgatc tggatgtccg gatataggtc aagaaattcg
      841 ccaagtcgcg gcgtgagcca atgcacgccg atgccaggtg tggttgagac cttgagttca
      901 ccattcggac gttcgcggct gtcggtaagc ttggtgcgcg ccgcttccag tttcatgaat
      961 acttcatgcg ccgtgcggta aagtaattcg ccctgctcgg tgaggatcag gccgcgtgca
     1021 tggcggtgga atagcgatac cgaaagctcg gcttccagag ccgaaacctg acgcgatacc
     1081 gccgactgtg acagtccgag ccgctcgcca gcgtgcgtaa acgagccggc ctcagctgcg
     1141 gcgtgaaaca ccttcagctt gtcccagtcc atcgagttgt tgggggccat ggtttattct
     1201 gctgccgcgc gctggtgctc gtgcgcggcc aagaattttt ctgcttcgag cgcggccatg
     1261 cagccctgcc cggcggcggt caccgcctgt cggtaaatat cgtcggtgac gtcgcccgct
     1321 gcgaacacgc cgggcaccga ggtgacggta gagtggccgg cggtgatgat atagccggat
     1381 ggcttcatct gaacctgacc ggcgaacaat tctgaggccg gcgagtggcc gatggcgatg
     1441 aatacgccgt cgacacggcg ttccgtgaca gcgctggtct tcacattttt gagccgcaca
     1501 ccgctgacct tcaacgggtt atcaccgccg gtgatgtctt cgagcacgct gtcccaaatc
     1561 acttcgatct tgggatgttt gaacaaccga tcctgcaaaa tcttctcagc ccgaaatgct
     1621 tcgcgtcggt gcaccactgt gaccttggaa gcaaaattgg tcaggaacag cgcctcttca
     1681 actgcggtgt tgccgccgcc gacgaca
//
LOCUS       DBNGZE010000109         8805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_223935, whole genome shotgun sequence.
ACCESSION   DBNGZE010000109 DBNGZE010000000
VERSION     DBNGZE010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_223935"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="AC16FV_02730"
     CDS             complement(<1..850)
                     /locus_tag="AC16FV_02730"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR2849012.1"
                     /translation="MPALGARQEFPPITPVFMKPLEDDLSQRRAQKLYRERMVLESPQ
                     GVEVRVGAETLLSFCSNDYLGLANDPRVIAAFQQGAQCYGVGAGASHLVSGHSRAHHA
                     LEEELADFVGARRALLFSTGYMANLGVLGALTDRNDAIFEDRLNHASLIDAARLARAK
                     VTRYPHADVDRLASLLDHAHRPALVTTDAVFSMDGDIAPLAGLSGLAARHEARLLVDD
                     AHGLGVLGQNGRGTLEYLGLALAPPVILMGTLGKALGVFGAFVAGEEALIETLIQRAR
                     TYIYTTA"
     gene            1019..1705
                     /locus_tag="AC16FV_02735"
     CDS             1019..1705
                     /locus_tag="AC16FV_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR2849013.1"
                     /translation="MLKARKWLWSGNCLLCHARIPAGTDLCAACERALPRTQNTCPRC
                     GTPDAVVGTDAVCGECQKQPPAYDGTRAVFRYASPLDRLIQHLKYHGRLELSRVLGGY
                     LAEHMLTLDDPLPDVVVPVPLHTTRLRERGYNQSLEIARFVCRRLRVPLDWQGAQRIR
                     PTAPQTELPRDQRRKNMRGAFTSSKAFAGQRVAIVDDVMTSGHTVNALAESLLQSGAK
                     EVRVWVVARA"
     gene            complement(1776..3185)
                     /locus_tag="AC16FV_02740"
     CDS             complement(1776..3185)
                     /locus_tag="AC16FV_02740"
                     /inference="COORDINATES: protein motif:HMM:NF020855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849014.1"
                     /translation="MSLINKMLQDLESRKNPQAGAINTKSVYEDLKPLSRAPSSRAPS
                     RRLVTVTALVAVVAAGAYAWTQWGDSFVSSLSSDKVAARPQPPVARKTIAAKPKPAPV
                     PEAAPVAVAAVQTVETQTSSATPTTPADKAADASAKPEQTQVTPPAVTETKKTETAPV
                     AATPKKATSMKSKATVARESGYWTVFPGETLYSISSQTGIDLGRLSSWNSLGREPVSH
                     PGQRLRLAPPAKTETRQAKTEKAVTPEKARTMVASAGATSIGEVQTTGNAVMDKKMKP
                     VSSDEKSETEYRRAVDLLEKGRMTDAEKHLRLALSASETHTQARELLAGLMLQQGHWR
                     DAQQLLEQGIDKVPAYYPFAQLLARIYVEHGAEQKALTVMEEGRRSAADNPDYVAFLA
                     ALYQRAGKNAEAVKTYNEAVTLNPQEGRSWLGLGISLEAMQDWNAAGAAYQRAIGSGT
                     LDDKLLKYARQRLAIVGNK"
     gene            complement(3182..4129)
                     /locus_tag="AC16FV_02745"
     CDS             complement(3182..4129)
                     /locus_tag="AC16FV_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR2849015.1"
                     /translation="MYGTHFGLKELPFTITPDTSFFFAHSSHQEALNTLLVAARSGEG
                     FMKVIGEVGTGKTLLCRKFLSALDHREFVTAYIPNPYLQPMTLLLAVADELDIKYPQH
                     INQHQLLKLLNRYLIDSYAEGKRVLVCLDEAQAIPIETLEGLRLLSNLETERRKLMQV
                     VLFGQPELNARLDNPSIRQLKQRISFACQLSPLNLSEVEFYISHRLAVAGYRGPRLFP
                     HKVVKRIYRTSNGIPRLVNILSHKALMAAFGEGARVVSERHVRMAVEDTESTQGARTV
                     KARLKRYLTALVSAVTVVSLLPLTTLALSGASLITGGLV"
     gene            complement(4161..5861)
                     /gene="mshL"
                     /locus_tag="AC16FV_02750"
     CDS             complement(4161..5861)
                     /gene="mshL"
                     /locus_tag="AC16FV_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HIR2849016.1"
                     /translation="MRSTNNIGMRIRRALVAALGMLLVGCQSVSPGWRDSVSASVDES
                     LSQARAGKDGKAIPSDVSKALLPPIEITLPQGGTVPLEPRFDLTVNNAPAHQVFMGLV
                     EGTPYSMILHPDVSGTISLNLKEASVPEALNVIRQVYGYEYRREGNRFFILGSDMQTR
                     MFPVNYLNLSRKGTSDTRVSASGLSATGATAGSTGTTTTTPAQGASGVQIQTQTQADF
                     WKDLTTALVAIVGSDGGRKVIVNPQAGIVVVRAMPDELRMVEDYLGVTQTSMNRQVIL
                     EAKIVEVQLKDGFQSGINWAGVAEKSGRTYTIGQVGGGKSLGNTGLSEIATNSGNLNP
                     TTGTFSAISGTNSSAFGGVFTLAVKSSNFAAFFELLKTQGDLNVLSSPRVSTMNNQKA
                     VIKVGTDSYFVTKQDTTASAVVGGSPTISTELSPFFSGIALDVTPQIDEAGNINLHIH
                     PSVSEVSEKLITVASNQTISTALSSVQESDNIVRATSGQIVVIGGLMKEASTDDNASV
                     PVLGDIPLIGNLFKHKKVTRIKRELVILLKPTMINVGQDWSDAAGESQERIKKIRIGS
                     "
     gene            complement(5858..6286)
                     /locus_tag="AC16FV_02755"
     CDS             complement(5858..6286)
                     /locus_tag="AC16FV_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849017.1"
                     /translation="MAGMKLDTGNDRQTGIRDIVLSAVIVMASLTGFMANALADDFAD
                     LTDPTQPLHGAAVAGAQRPPGSALQSTFVSASQRRAVINGRTYKVGDKYGGGMIVDIQ
                     PYEVVVKQADRETRMRLLPKLAKEIRMVKVPANSQEGRQK"
     gene            complement(6292..6960)
                     /locus_tag="AC16FV_02760"
     CDS             complement(6292..6960)
                     /locus_tag="AC16FV_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849018.1"
                     /translation="MISDALKKQFQGAANRIDAMSLRERALIFITLLVVLYFLAVNVL
                     FGPLNTEKDRLQQQLNQKRAETRALEMQVQALVGNGEDPDAAKRKKLESLQENLKTMD
                     AELMRVTSGLVTPKEMARLVEQMLLKNRGLQVVKVESVPATPLLNVIENAISSGPMVY
                     KHGMKIEIKGGYLDILRYLKSLETLPWKVFWGKVTMKTEKYPDSRVSVLIYTLSTREA
                     WIGL"
     gene            complement(6963..7580)
                     /locus_tag="AC16FV_02765"
     CDS             complement(6963..7580)
                     /locus_tag="AC16FV_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR2849019.1"
                     /translation="MSQQINLYQPIFRKEEKKFSTVAMLQAMGLVALGVVALYAFSWW
                     QSQTLKNELKRVEQNHVTASKRLADASQKFGPRTGVTSLDAEIARLEAEVAARQQVQE
                     ILRRGIFSNTRGFSDYFASFARQRVPGVWLTGFDITGAAEEMSLAGRTTNPELVPRYM
                     QKLSSEKTLAGIEFRAFQMNRPEPDAKGPDAMFVEFQARTAANPG"
     gene            complement(7577..8485)
                     /locus_tag="AC16FV_02770"
     CDS             complement(7577..8485)
                     /locus_tag="AC16FV_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HIR2849020.1"
                     /translation="MLPGLTGVGLRSDGVCVVRVERSAGRAPTLTLVDFRPWGDQGQE
                     KVLARVAADYDLGRSRCTTVLDASEYSLLLTEAPDVPPDELRAAIRWRIKDLIDFHIN
                     DATLDVFDLPGEKAAGRARSMYAVAAHSSAIQKRADMMSAASINLDVIDIPEMAQRNL
                     ASLLPEDAKGVVLLSFTTGGGLITISKQSEIYLSRNIDIGLDMMASFQDSNELFDRIA
                     LEVQRSLDYYDSHFRQAPITTLALAPMPREVPGLVDYLKANLSANVITMDLTTLMECE
                     VDLKPELQSACLAVLGAALRQEERAL"
BASE COUNT         1680 a         2697 c         2572 g         1856 t
ORIGIN      
        1 gcgcggtggt atatatatag gtacgcgccc gctggatcag cgtctcgatc aaggcctcct
       61 ccccggccac aaaagccccg aaaaccccca aggctttgcc caaggtgccc atcagaatca
      121 ccggtggcgc cagcgccagg cccagatatt ccagcgtgcc gcggccgttc tggcccagca
      181 cgcccaaacc gtgggcatcg tccaccagca agcgcgcctc atgtctggcc gccagacctg
      241 acagcccggc caatggcgcg atgtcgccgt ccatgctgaa aaccgcgtcc gtcgtgacaa
      301 gggcgggccg gtgcgcgtga tccagcaggc tggctagccg gtccacgtcc gcatgcggat
      361 agcgcgtcac cttggcgcgc gccagacgcg ccgcatctat cagcgaagca tgattcaggc
      421 gatcctcgaa aatcgcatcg ttgcggtccg tgagcgcgcc cagcacgccg aggttcgcca
      481 tatagccggt ggagaacaac aaggcgcgcc gggcgccgac aaaatccgcc agctcctctt
      541 cgagcgcgtg atgcgcgcgg ctgtggccgc tgacgagatg cgaggccccg gcgcccacgc
      601 catagcactg cgcgccctgc tggaaggccg cgatcacccg cgggtcattg gccagtccca
      661 gatagtcgtt gctgcagaag gacagcagcg tttccgcgcc gacacggacc tccacgccct
      721 ggggcgattc gagcaccatg cgttcgcgat acagtttctg cgcgcggcgt tgtgacagat
      781 cgtcttcgag tggtttcatg aaaaccgggg tgatgggtgg aaattcctgg cgcgcgccga
      841 gtgcaggcat gcggccaaat ttgtcagaaa agccgtcctg ctcgctacgc tcgttcgtct
      901 tgccgtgaca aagtagccct gctcactgcg ttcgctcgtc ttgctgtaat aattaatatg
      961 cggcagtgta gtgcttcccc gatatcagtc aacatgaaac atcacaggaa gttgacattt
     1021 gcttaaagca cgaaaatggc tgtggtccgg gaattgcctg ctgtgccatg cgcgaatccc
     1081 ggcgggaacg gatttatgcg cagcctgcga gcgggcgttg ccacggacac aaaacacctg
     1141 tccgcgctgc ggcacaccgg acgctgtcgt cggcaccgat gccgtctgcg gcgaatgcca
     1201 gaaacagccg cccgcttacg acggcacgcg tgcggtgttc cgctatgcct caccgctgga
     1261 ccggctgatt caacatctca aataccatgg tcgcctggaa ctgtcgcgcg tgctcggagg
     1321 gtatctcgcg gaacatatgc tgacgctcga tgatccgctt cccgacgtcg tggtaccggt
     1381 cccgttacat acgacgcgcc tgcgcgagcg cggctacaac cagtcgttgg agatcgcgcg
     1441 gtttgtctgc aggcgtttgc gagttccgct cgattggcag ggtgcgcaac gcattcgccc
     1501 gacggcgccg caaacggaat tgccgcgcga tcagcgacgc aaaaacatgc gcggggcatt
     1561 cacgtccagc aaggcgttcg ccggacagcg tgtcgccatc gtcgatgacg tgatgaccag
     1621 cggacacacg gtgaacgccc tcgcggagag tctgctccag agcggagcga aagaagtgcg
     1681 ggtgtgggtg gtggcgcggg cttgaaacag tttcgagttt ctggttttta atttctggtt
     1741 ctttaaacca gaaacttgta actaaaaact ggaaactatt tgtttcctac tatcgccaga
     1801 cgctggcgcg cgtatttgag cagtttgtcg tccagcgtgc cgctgccgat ggcgcgttga
     1861 tatgccgcgc cggcggcgtt ccagtcctgc atggcttcca gcgagatgcc cagacccagc
     1921 cacgagcgtc cttcctgtgg attgagcgtg accgcttcat tataggtctt gacggcttcg
     1981 gcattcttgc cagcgcgctg atatagcgcg gccaggaacg ccacataatc gggattatcc
     2041 gcagctgagc gacgaccttc ttccatgacc gtcagggcct tctgttccgc accatgctca
     2101 acataaatcc gcgccagcag ttgcgcgaac ggataatagg ccggcacctt gtcgatgcct
     2161 tgctccagaa gctgctgcgc atcacgccag tgtccttgct gcaacatcag accagccagc
     2221 agttcgcgtg cctgcgtatg cgtctcgctg gcactcaggg ccagcctcag gtgtttttcg
     2281 gcatcggtca tgcgtccctt ctccagcaga tcgaccgcgc ggcgatattc ggtttctgac
     2341 ttttcgtccg aactgaccgg tttcatcttt ttgtccatga ccgcattgcc ggtagtttgg
     2401 acttctccga tggatgtcgc tccagcggac gcaaccatcg ttctcgcctt ttctggcgtg
     2461 acagcctttt ctgttttggc ctgtctggtt tcggttttcg ccggcggcgc caggcgcagc
     2521 cgctggccgg gatgactcac aggctcgcgg cccaagctgt tccagctgga cagacgcccg
     2581 agatcgatgc ctgtttggct ggaaatgctg tacagcgtct cgccagggaa cacagtccaa
     2641 tagccggatt cccgcgcgac agtggctttg gatttcatcg atgttgcctt tttgggcgtg
     2701 gcagcaacgg gcgccgtttc ggtttttttg gtttccgtca cagcgggtgg cgtgacttga
     2761 gtctgctccg gcttcgcgga tgcatcagcc gccttgtcgg ccggtgtggt cggtgtcgcg
     2821 gacgaagttt gtgtctcgac agtctgcacg gctgcaaccg ccactggtgc agcttcaggg
     2881 accggggccg gtttgggttt ggctgcgatt gtcttgcgcg ccaccggcgg ctgcggcctg
     2941 gcagcaactt tgtcagaaga caaactcgaa acgaaactgt ctccccactg cgtccaggca
     3001 taggcgccgg cagcgacaac cgcaaccagc gccgtcaccg tcaccagacg acgcgagggt
     3061 gcgcgtgacg acggggccct gctgagcggc ttgaggtctt cgtaaacgga cttcgtgttg
     3121 atcgctccgg cctggggatt cttgcgcgac tccaggtcct gaagcatctt gttgatcagg
     3181 ctcacaccaa cccacctgtg atgagcgatg cgccggacag cgccagggtg gtcagaggca
     3241 gcaggctcac gaccgtcacc gccgacacga gcgccgtcag atatctcttg agccgggcct
     3301 ttaccgtgcg cgcgccctga gtggactcgg tgtcctcgac cgccatgcgc acatgccttt
     3361 cggacacgac gcgcgcaccc tcaccgaacg ccgccatgag ggccttgtgc gagagtatgt
     3421 tcaccaggcg cgggataccg ttgctggtgc gatagatccg cttgaccacc ttgtgcggga
     3481 acagacgcgg gccgcggtaa ccggccacgg ccagacgatg cgaaatatag aattcgacct
     3541 cgctcaggtt gagcggcgaa agctggcagg caaaactgat gcgctgcttg agctggcgga
     3601 tcgaggggtt gtcgaggcgc gcgttcagct ccggctgacc gaacagcacc acctgcatca
     3661 atttgcggcg ctccgtctcg agattcgaga gcaggcgcaa accctccagg gtttcgatcg
     3721 ggatagcctg ggcctcgtcc agacagacca gcacacgctt gccttcggcg tagctgtcga
     3781 tgagataacg attcagcagc ttgagcagct ggtgctggtt gatgtgttgc ggatacttga
     3841 tgtccagctc gtcagcaacc gcgagcagca atgtcatcgg ctgcagatag ggattcggga
     3901 tataggcggt gacgaactcg cgatgatcga gcgcgctcag gaacttgcgg catagcagcg
     3961 tcttgccggt gccgacctcg ccgatcacct tcatgaagcc ctcgccactg cgcgcggcca
     4021 ccagcagcgt gttcaacgcc tcctggtgcg acgaatgggc gaagaaaaag ctcgtgtccg
     4081 gcgtgatggt gaacggcagt tccttcagcc cgaaatgtgt tccgtacatg tgcctttacc
     4141 cgcttctcaa aagtattcgt tcaggatccg atgcggattt tcttgatgcg ctcctgcgac
     4201 tcgccggcgg cgtcgctcca gtcctgaccg acgttgatca tggtcggctt tagcagaatc
     4261 accagctcgc gcttgatgcg cgtcaccttc ttgtgcttga acagattgcc gatcagcgga
     4321 atatcgccaa gcacgggcac ggaggcgttg tcatcggtgc tggcctcctt catcagcccg
     4381 ccgatcacga cgatctggcc gctggtggcg cgcacgatgt tatccgactc ctgtactgaa
     4441 ctcagtgccg tcgaaatcgt ctggttcgag gcgacggtaa tcagtttctc ggacacttcg
     4501 ctcaccgagg gatgaatatg caggttgatg ttgccggcct catcgatctg tggcgtcaca
     4561 tccagcgcaa tgccggagaa gaaaggcgac aattccgtcg atatggtcgg cgaaccaccc
     4621 accacagcgc tcgccgtggt gtcctgcttg gtcacaaaat aggagtccgt gcccaccttg
     4681 attaccgctt tctggttgtt catggtcgag acacgcgggc tggaaagcac gttcaaatcg
     4741 ccctgcgtct tcagtaattc aaaaaacgcc gcgaaattgc tggacttcac cgccagcgtg
     4801 aacacaccac cgaaggcgga actgttggta ccggaaatgg cgctgaaggt gccggtggtg
     4861 ggattcagat tgccagagtt ggtggcaatc tcgctgagcc ccgtattgcc aagcgatttc
     4921 ccgcctccga cctgtccgat cgtataggta cggccactct tttcagcgac cccggcccag
     4981 ttgatgccgc tctggaaacc atccttgagc tgcacctcga caatcttggc ttcgaggatc
     5041 acctgacgat tcatgctggt ctgggtgacg cccagatagt cttccaccat gcgcaactca
     5101 tctggcatgg cgcgcaccac gacaatgccg gcctgcggat tgacgatcac ctttctcccg
     5161 ccgtcgctgc cgacgatggc caccagcgcg gtcgtcagat ccttccagaa atcggcctgt
     5221 gtctgtgttt gaatctgaac accgctagct ccctgagcgg gcgtggtggt tgtcgtgccc
     5281 gtagaaccgg ctgttgcacc ggtggcactc agaccgctcg ccgacacgcg cgtgtcggac
     5341 gtgcccttgc gcgagagatt gagataattc accgggaaca tgcgcgtctg catgtcagag
     5401 ccgagaatga aaaagcggtt gccctcgcgc cggtactcat agccgtaaac ctggcggata
     5461 acgttcagcg cttccggcac actggcttcc ttgaggttga gcgagatcgt cccgctcacg
     5521 tccggatgca ggatcatgct gtacggcgtg ccctcgacca gccccatgaa cacctggtgc
     5581 gcgggtgcgt tattcacggt gagatcgaaa cgcggttcga gcgggacggt accgccctgg
     5641 ggaagcgtga tttcgatggg cggcagcagc gccttgctta cgtccgatgg aattgccttg
     5701 ccgtccttac ccgcgcgcgc ctgtgagagg ctctcgtcca cgctggcact cacggaatcg
     5761 cgccacccgg gggaaacact ctgacacccc acaagcaaca tacccagcgc cgcgaccagc
     5821 gcgcgccgga ttctcatccc gatattattc gtacttctca tttttgcctg ccttcctggc
     5881 tgtttgccgg taccttgacc atgcgtatct ctttcgccag ttttggtaac aaacgcatgc
     5941 gcgtttcacg atccgcctgc ttcaccacta cttcatacgg ctgtatatcc acaatcatcc
     6001 cgccaccata cttatcgccg actttgtaag tccggccgtt gattacggcg cgtcgttggc
     6061 ttgccgagac gaaggttgac tgcaacgccg aacctggcgg cctctgcgcg ccggcgacag
     6121 cagcgccatg caacggctgc gtcgggtccg tgagatcggc gaaatcatcc gccaaggcat
     6181 tcgccatgaa ccccgtcaaa ctggccatca caatcaccgc cgacaggaca atatcgcgaa
     6241 taccggtttg cctgtcattt cctgtatcaa gtttcatgcc agccatgacc gtcacagccc
     6301 aatccaggct tcacgcgtgc tgagggtgta aatcagcacg ctgacgcggg aatcgggata
     6361 tttttccgtc ttcatggtta ccttgcccca gaagactttc cagggcagag tctccagaga
     6421 cttgagatag cgcaggatgt ccagatagcc gcccttgatc tcgattttca tgccgtgctt
     6481 gtaaaccatg gggcccgaac tgatggcgtt ttcgatcaca ttcagcaacg gggtggccgg
     6541 gacgctttcc acttttacga cctgcaggcc acggttcttg agcagcattt gctcgaccag
     6601 ccgcgccatt tccttgggcg tcaccaggcc ggacgtgacc cgcatcagtt ccgcatccat
     6661 ggttttcagg ttttcctgca acgactcgag ctttttgcgc ttggcggcat cgggatcttc
     6721 tccgttaccc accagcgcct gcacctgcat ttccagcgcc cgggtttccg cgcgtttctg
     6781 gttaagctgt tgctgcaacc tgtccttctc ggtgttcagg ggaccaaaca agacgttcac
     6841 cgccaggaaa tacaacacca ccagcagcgt gatgaaaatc agggcgcgtt cgcgcaacga
     6901 catggcgtcg atccggttag cggctccctg gaactgtttt ttcagagcgt cggatatcat
     6961 ggctaccccg gattggcggc ggtcctggcc tggaattcga cgaacatcgc atccgggccc
     7021 ttcgcatccg gctccggccg gttcatctga aatgcccgga actcgatccc ggcgagcgtc
     7081 ttttccgacg agagcttctg catgtagcgc ggcaccagct ccggattggt cgtgcggccg
     7141 gccagcgaca tttcttccgc ggcaccggtg atgtcaaatc ccgtcagcca cacccctggg
     7201 acacgctggc gagcgaacga ggcgaagtaa tccgagaatc cgcgggtgtt gctgaaaatg
     7261 ccgcgccgga gaatttcctg gacctgctgt ctcgcggcaa cctcggcttc caggcgcgcg
     7321 atctccgcgt caagcgaggt gacgcccgtg cgcgggccga acttctggct cgcgtcggcg
     7381 aggcgcttgc tggccgtgac gtgattttgc tccacccgct tgagttcgtt tttcagtgtc
     7441 tggctttgcc accagctgaa ggcatacagc gccaccacac cgagcgccac cagacccatg
     7501 gcctggagca tggccaccgt tgagaatttc ttctcttcct tacggaagat tggctggtaa
     7561 agattgatct gctggctcac agggccctct cctcttgccg cagggccgcg cccagcacgg
     7621 ccagacaggc cgattgaagc tcgggcttga gatccacttc gcattccatg agcgtcgtca
     7681 ggtccatcgt gatcacgttg gcgctgagat tggccttgag gtaatccacg agtcccggca
     7741 cttcccgggg catgggcgcc agcgccagcg tcgtgatggg cgcctgacgg aagtgactgt
     7801 cgtaataatc gagcgagcgt tgcacttcga gcgcgatgcg gtcgaacaat tcgttggagt
     7861 cctgaaacga cgccatcatg tcgagcccga tgtcgatgtt acggctgaga taaatttccg
     7921 attgcttgct gatagtgatc aaaccgccac cggtcgtgaa tgacagcagc acaacaccct
     7981 tggcatcttc cggcaacagc gaggccagat tacgctgcgc catctcggga atatcgatga
     8041 catcgaggtt gatactggcg gcgctcatca tgtccgcgcg tttctgaatg gcgctgctgt
     8101 gcgccgccac ggcgtacatc gagcgcgcgc ggccggcagc cttctcgccc ggcaggtcga
     8161 agacgtcgag cgtggcgtcg ttgatgtgaa aatcaatgag gtccttgatg cgccagcgga
     8221 tagcggcgcg caactcgtct ggcggcacgt ccggcgcctc ggtcagcagc agcgaatact
     8281 cgctggcatc gagcacggtg gtgcagcgcg aacgcccgag atcgtaatcg gccgccacgc
     8341 gtgccagcac cttctcttgg ccctggtcgc cccagggacg gaaatccacc aacgtcagcg
     8401 ttggcgcacg tccggcgctg cgctcgacgc gcaccacgca gacgccgtcg gaccgcagcc
     8461 ccacgccggt cagaccggga agcattttct tttttagaaa cggtaacagg ggagatctcc
     8521 ttttccgaat gagtcccttt tactatgcaa aaatgcggcc aaactctcag acatcagcaa
     8581 tattgcttga ttttctgagg tttaagttaa gaatcggtca ccaactatct gttgtcaatg
     8641 cggtttttaa caatacaagt ccgaagaaaa aagcggaaaa tcaaggaaaa tcaggataag
     8701 acggctcaag agggccgggc agcgcttagc tcccacggga aaaattttta ggggaacacc
     8761 tcgcgccagg caatgatcgg cgtgcgccgt tcggtagcaa gagcg
//
LOCUS       DBNGZE010000110         5251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_639693, whole genome shotgun sequence.
ACCESSION   DBNGZE010000110 DBNGZE010000000
VERSION     DBNGZE010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_639693"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="AC16FV_02775"
     CDS             <1..1093
                     /locus_tag="AC16FV_02775"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:NF040246.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR2849021.1"
                     /translation="DAKIAWLRSLLEWKDEVASASDFVDQFKSEVFSERVYVFTPKGN
                     IADLPAGSTPLDFAYHIHTNVGHRCRGAKVNGQMVPLTHALTTGDRVEVLTVKEGGPS
                     RDWLSPHLGYLHTSKARSRVNHWFREQNYEASVSEGRTVLEREFHRLGISDVKFEKLA
                     QRFKFQKVDDFLEAAGRGDIKPVQIMNALQEMLGQDFRAPTPVVAPRAPGAPAKPSGV
                     TIQGIGNLLTRMAKCCNPLPGDPIVGFITRGQGVTIHRRDCTNALRHHDEHNERLIEV
                     SWGAQAGVAYPIEVEVTAYDRAGLLRDITSLLANEKINVLGVSTLTDRQQVAHMTFTL
                     EIPNIETLSRILALLDQIPNVVGVRRRAK"
     gene            complement(1128..1328)
                     /locus_tag="AC16FV_02780"
     CDS             complement(1128..1328)
                     /locus_tag="AC16FV_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HIR2849022.1"
                     /translation="MSIEQFESWSLVIGIGGLIVWMLLIIWKMGQESQAGKYGYFVLF
                     LALGLGFVGFIAKTILVEVLKL"
     gene            complement(1416..3155)
                     /locus_tag="AC16FV_02785"
     CDS             complement(1416..3155)
                     /locus_tag="AC16FV_02785"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR2849023.1"
                     /translation="MRVSCFLLSTVRETPAEAETISHKLMLRAGMIRKLAAGIYTWLP
                     LGLRVLRKVEQIVREEMNRAGAQEVLMPAVQPAELWQESGRWDQYGPELLRLTDRHKR
                     DFCFGPTHEEVITDLIRREIKSYRQLPANFYQIQMKFRDEIRPRFGVMRAREFLMKDA
                     YSFHINETSLGETYKVMHETYSRIFQRVGLEFRPVAADTGAIGGSNSHEFHVLADSGE
                     DAIAICSKCDYAANVEMAQALPPLGKRPAPGKAMQTVDTPGQHTIEQVSKFLKVDPKQ
                     TIKTLLVKGTGGVIALVLRGDHELNEIKVAKLPQVAKPLMFVTPGEVELAAGCEPGSI
                     GPVGLKITVIADESAARLADFVCGANVNGKHLINVNWGRDAAEPEVADLRKVVEDDPC
                     PDSGTGQCGGTLSIRRGIEVGHIFQLGTKDSEAMQATVLDETGQPVVMPMGCYGIGVS
                     RVVAAAIEQNHDDNGIIWPDAIAPFQIALVPIGYGKSEKVRETIDRLYGELVHAGFDV
                     LLDDRDERPGVIFADMDLIGIPHRLVIGDKGLAKGAAEYKGRRDKTAREVPLDQIVST
                     LRETIKSRDAGQF"
     gene            complement(3254..3739)
                     /locus_tag="AC16FV_02790"
     CDS             complement(3254..3739)
                     /locus_tag="AC16FV_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR2849024.1"
                     /translation="MTRTMVFALSFGLLAGASFPALAAEPDHLKLYTTKRNFDTVKED
                     IEIAITNRGLVIDHTSHIGAMLERTGKDLGTTKPIYGNAGSMQFCSATVSRRTMEADP
                     TNIVFCPYIIVYYTLHQDPKTVYVGYRRPVPVGSEASRASIKEIEDLLDGIVKEALNI
                     K"
     gene            complement(3856..4257)
                     /locus_tag="AC16FV_02795"
     CDS             complement(3856..4257)
                     /locus_tag="AC16FV_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849025.1"
                     /translation="MKRTLIYAALLAAMLPAWGTAAYAADQDQDRDRLQKQDQTRDQD
                     QIYGSQLMTQQERNEYRNRMRAAKTEQEREQIRKEHHEQMKIRAKEKGMTLPDEPPAR
                     GRGMGPGRGMGPGMGPGGGGMGPGMGNNRRY"
     gene            complement(4476..>5251)
                     /locus_tag="AC16FV_02800"
     CDS             complement(4476..>5251)
                     /locus_tag="AC16FV_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR2849026.1"
                     /translation="LQAIAALVRDVEAQLGVTATVGIGIPGVISPATGLVKNANSIAL
                     NGHTFDRDISALLGREVRVENDANCFALSEALDGAGKNYGAVFGVILGTGVGGGIVFH
                     GKLHEGPQHIAGEWGHNVLEADGPPCYCGKRGCVETFLSGPGLAHDWRRHGGDSLFEA
                     KDIVVLAEQGDARANAALQRYLYRFGKALSVVINILDPDAIVLGGGMSNISRLYTDGR
                     DSVARHVFNDELHTKILPHVHGDSSGVRGAAHLWAPQDS"
BASE COUNT          976 a         1576 c         1637 g         1062 t
ORIGIN      
        1 cgacgccaag atcgcctggc tgcgcagcct gctggaatgg aaggacgagg ttgcgagtgc
       61 cagcgatttc gtggaccagt tcaagtccga ggtgttcagc gagcgtgtgt acgtattcac
      121 gcccaagggc aatatcgcag acctgcccgc cggcagcacg ccgctcgatt tcgcgtatca
      181 catacacacc aacgtcgggc accgttgccg cggcgccaag gtcaacggcc agatggtgcc
      241 gctgacgcac gcgctcacca ccggcgaccg tgtcgaggtg ctgaccgtca aggaaggcgg
      301 gccgtcgcgc gactggctgt cgccgcattt gggttatctg catacctcca aggcgcgctc
      361 gcgcgtgaac cactggttcc gcgaacagaa ttacgaggcc agcgtttccg aggggcgtac
      421 cgtgctggag cgcgagtttc atcgcctcgg tatcagcgac gtcaagttcg agaagctggc
      481 gcaacgtttt aaattccaga aagtcgatga ctttctggag gccgccggcc gcggtgacat
      541 caagccggtg cagatcatga acgccttgca ggaaatgcta ggacaggatt ttcgtgcacc
      601 gacaccggtt gtcgcgccac gggcgccggg cgcgccggcc aagccatccg gcgtgacgat
      661 tcagggcatt ggcaatctgc tgacgcgcat ggcgaaatgc tgcaatccgc tgccgggcga
      721 tccgatcgtc ggcttcatca cgcgtgggca gggcgtgacc attcaccgtc gcgactgcac
      781 caatgccttg cgccatcatg acgaacacaa cgagcgcttg atcgaggtca gctggggcgc
      841 acaagcgggt gtggcctacc cgatcgaggt ggaggtgacg gcctatgacc gcgcgggact
      901 gctgcgcgac attacgtcat tactggcgaa cgaaaagatc aacgtgctag gcgtcagcac
      961 gcttaccgac agacagcagg tggcgcacat gacctttacg ctcgaaatcc cgaatattga
     1021 aacactatcg cgcattcttg cgctgctgga tcagattccg aacgttgtcg gggttcgccg
     1081 tcgcgccaaa taactttcct gcctgatacc gcaggtgcgt atgttggcta aagcttgagc
     1141 acttcaacga ggattgtctt ggcgatgaaa ccaacgaagc ccagaccgag cgccaggaac
     1201 aagacgaaat agccgtactt gccggcctgt gattcctgcc ccatcttcca gatgatcagc
     1261 agcatccaga caatgagacc gccgataccg atgacaagag accaggattc gaattgttcg
     1321 atggacatga tgcgccgcca gggtgaaaaa cggcgcgcat catacacagg cccgacatgc
     1381 ttcgcaaatg accgcgatca agcgcggcgg ttttttcaaa actggcctgc gtctcgggat
     1441 tttatcgttt cgcgtagtgt gctgacgatc tgatccagtg gtacttcgcg cgctgtcttg
     1501 tcgcgccgtc ccttatactc ggccgcaccc ttggcgagac ccttgtcgcc gatgacaagg
     1561 cgatgcggaa tgccgatcag gtccatgtcc gcgaaaatga cgccgggacg ctcgtcgcgg
     1621 tcgtcaagca gcacgtcgaa accggcgtgc accagctcgc catagagccg gtcgatggtt
     1681 tcgcggacct tttctgattt tccataaccg atgggcacca gcgcgatctg gaatggcgcg
     1741 atggcgtcgg gccagatgat gccattgtca tcgtggttct gttcgatggc ggcggcgacc
     1801 acgcgcgaga cgccgatgcc gtagcagccc atgggcatga ccaccggctg gccggtttcg
     1861 tcgagcacgg tcgcctgcat cgcctcgctg tccttggtgc cgagctggaa gatgtggccg
     1921 acctcgatgc cgcgccggat cgatagcgtg ccgccgcact ggcccgtgcc ggaatcgggg
     1981 caggggtcgt cttcgaccac cttgcgcaga tcggccacct ccggttcagc cgcgtcgcgc
     2041 ccccaattaa cattaataag gtgtttgccg ttgacgttcg cgccacagac gaaatcggcc
     2101 aggcgcgcgg cggattcatc ggcgatgacg gtgatcttca ggcccaccgg tccgatcgag
     2161 ccgggttcgc agccggcggc cagttccact tcgccgggtg tgacgaacat caatggtttc
     2221 gccacctgcg gcagtttcgc caccttgatc tcgttcagct cgtgatcacc gcgcaacacc
     2281 agcgcgatga cgccgccggt gcctttgacg agcaacgtct tgatcgtctg tttcggatcg
     2341 actttcagga acttgctgac ctgctcaata gtatgttgtc ccggcgtgtc cacggtctgc
     2401 atggccttgc ccggcgccgg gcgcttgccg agcggcggca gcgcttgggc catttccacg
     2461 ttggcggcat agtcgcattt cgaacagatg gcgatggcgt cctcgccgga atcggccagt
     2521 acatggaatt catgcgagtt actgccgccg atggcgccgg tgtcggccgc gacgggacgg
     2581 aattccagcc ccacgcgctg gaatatgcgc gagtaggttt cgtgcatgac cttgtaggtt
     2641 tctcccagcg aggtctcgtt aatatggaac gaataggcat ccttcatgag gaactcgcgt
     2701 gcgcgcataa cgccgaagcg cgggcggatt tcgtcgcgga atttcatctg gatctggtag
     2761 aaattcgccg gcagttgccg gtagctcttg atctcgcgcc ggatgagatc ggtgatgacc
     2821 tcctcgtgcg tgggaccgaa gcagaaatcg cgtttgtgcc ggtcggtcag gcgcagcagc
     2881 tccgggccat actggtccca gcgcccggat tcctgccaca gctccgccgg ctgtaccgcc
     2941 ggcatcagca cttcctgcgc gccggcgcgg ttcatttcct cgcgcacgat ttgctccacc
     3001 ttgcgcagca cccgcaggcc gagcggcagc caggtgtaga tgccggcggc gagcttgcgg
     3061 atcatgcccg cgcgcagcat gagtttgtga ctgatggttt cggcctcagc cggggtttcg
     3121 cggacagtgg agagcaagaa acaggaaacg cgcatggatc gttattctcg tggatcggac
     3181 tcggaaacgg gagtggagta taagtcagga ctgcggcgcg ccaaagcgcc gcgacgtaca
     3241 agacgaatcg ggcttatttg atgtttagtg cctctttcac gattccatcg agcaggtctt
     3301 caatctcctt gatcgaggcg cgggaggctt cggaacccac cggaaccgga cggcggtaac
     3361 cgacatacac cgtcttcggg tcctgatgca gggtgtagta cacgatgata tagggacaga
     3421 agacgatgtt ggtgggatcg gcctccatgg tgcggcgcga aaccgtggcc gagcagaatt
     3481 gcatcgagcc agcgttaccg taaatcggtt tggtggtgcc aaggtccttg ccggtgcgtt
     3541 cgagcatggc gccgatatgc gaggtgtggt cgatcaccag gccgcggttg gtgatggcga
     3601 tctcgatgtc ttccttgacg gtatcgaaat ttcgcttggt ggtataaagc ttgagatgat
     3661 ccggttcggc ggccagggcc gggaatgatg cgccggccag caggccgaac gaaagggcga
     3721 acaccatagt acgggtcatt tcatctccag aatgccttga atcacgatgg cgcagtatat
     3781 cgcacgcgcg gaattcttcc cccgcccgga cgggcgaggg aagagagacg cgcttgtgca
     3841 gagattaatt ccggatcagt aacggcggtt gttgcccatg cctgggccca tgccaccgcc
     3901 accgggtccc atgccggggc ccattcctct gcccggaccc atgccgcgcc cacgagcagg
     3961 cggttcatcc ggcagggtca tgcccttctc cttggcgcgg attttcatct gctcgtgatg
     4021 ttccttgcgg atctgttcgc gttcctgctc ggtcttcgcg gcgcgcatgc ggttacggta
     4081 ctcgttacgc tcctgctggg tcatgagctg ggagccgtag atctgatctt ggtcgcgggt
     4141 ttgatcctgt ttttgcaggc gatcccgatc ctgatcttgg tccgcggcat acgctgctgt
     4201 tccccacgcc ggcagcatgg ccgcgagcag ggcggcataa atcaatgtgc gtttcatgac
     4261 agttctcctt aacatttcac ttggattgaa gggattcccc cgatccttgc tggttcaata
     4321 cccccattcg aggcgggata ccatgacggc tatcaaatat cgcgcaagca ccgcaggtac
     4381 atgtaaaaac gcatgagtgc tcccgcggtg caccgacacc gtgcggttta ttgccgcatg
     4441 ccgtcgcgga tgttctggtg acgcagatag cgcgctcagc tgtcttgcgg cgcccagaga
     4501 tgggccgcgc cgcgcacgcc ggagctgtcg ccgtggacat ggggcaggat tttggtgtgc
     4561 agttcgtcgt tgaatacatg gcgtgccacg ctgtcgcgcc cgtcggtgta gagccgtgaa
     4621 atgttcgaca tgccgccgcc gagcacgatc gcatccgggt cgaggatgtt gatgacgacc
     4681 gacagggcct tgccgaagcg gtagaggtaa cgctgcaggg cggcattggc ccgggcgtcg
     4741 ccctgttccg ccagcaccac gatgtctttg gcctcgaaca gggaatcacc accgtgccgg
     4801 cgccagtcat gcgccaggcc gggcccggaa agaaaggttt ccacgcagcc gcgcttgccg
     4861 cagtaacagg gcgggccgtc ggcttcgagg acgttatgcc cccattcgcc ggcgatgtgc
     4921 tgcgggcctt catgcagttt cccgtggaat acaatgccgc cgcccacgcc cgtgcccaga
     4981 atcacgccga acaccgcccc gtaatttttt cccgccccgt cgagcgcctc ggacagcgca
     5041 aagcagttgg catcgttctc cacccgcact tcgcggccga gcagggcgct gatgtcacgg
     5101 tcgaaggtat ggccgttcaa cgcgatcgag ttggcgttct tcaccagccc ggtcgccggg
     5161 ctaatcacgc cggggatgcc gatgccgacg gtggcggtga cgcccagttg cgcttccaca
     5221 tcgcgcacca gggcggctat tgcctggaga g
//
LOCUS       DBNGZE010000111         8204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_293578, whole genome shotgun sequence.
ACCESSION   DBNGZE010000111 DBNGZE010000000
VERSION     DBNGZE010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_293578"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="AC16FV_02805"
     CDS             complement(<1..411)
                     /locus_tag="AC16FV_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR2849027.1"
                     /translation="MFRISQYMQEIKQPTPLGPKRHPPGPVVIWNLIRRCNLTCKHCY
                     SISADTDFPGELSTAEVYKVMDDLKGFRVPVLILSGGEPLMRPDVFDISRRAKQMGFY
                     VGLSTNGTLIDESNIERIAAIGYDYVGISIDGLRE"
     gene            complement(493..966)
                     /gene="ahbB"
                     /locus_tag="AC16FV_02810"
     CDS             complement(493..966)
                     /gene="ahbB"
                     /locus_tag="AC16FV_02810"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
                     /protein_id="HIR2849028.1"
                     /translation="MDVLDRRIIVATQENLPRVSRPYHAIAEQLGISVGEVMARLQHM
                     LDSGVIRRIGAVPNHYLLGYKANGMSVWDVPEERIRELGQKIGALDFVSHCYHRPRHL
                     PDWPYNLFAMVHGHDRAEVGAKVAQIAALLGAADRGHTVLYSTRILKKTGLRISV"
     gene            complement(1092..1535)
                     /locus_tag="AC16FV_02815"
     CDS             complement(1092..1535)
                     /locus_tag="AC16FV_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR2849029.1"
                     /translation="MDTTDRHIINNLQGGFPLSERPYAEAAAKLGLTEDDLIRRLNDM
                     LEKGALSRFAPMYHAERLGGALTLAAMKIPPQDFERVAEIVNGFPEVAHNYAREHEFN
                     MWFVLATETPQRINEVIGEIEKATGYQVYNMPKLEEFFVGLRFEV"
     gene            complement(1535..2080)
                     /gene="ahbB"
                     /locus_tag="AC16FV_02820"
     CDS             complement(1535..2080)
                     /gene="ahbB"
                     /locus_tag="AC16FV_02820"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
                     /protein_id="HIR2849030.1"
                     /translation="MELNVIKHSAATLRRPQVDEADRRLLAAIQHGLPLTPRPYAEIA
                     AGLGISEMEVVARLAWLKEAGVIRRFGVVVRHHELGYAANAMVVWDVPDEQVTELGHC
                     LAGFDFITLCYRRPRHLPQWRYNLYCMIHGKSREEVLTHLEWMVNHCGLQSLPHEVLF
                     SRRRFKQRGAVYARTAGVEKR"
     gene            complement(2071..2532)
                     /locus_tag="AC16FV_02825"
     CDS             complement(2071..2532)
                     /locus_tag="AC16FV_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HIR2849031.1"
                     /translation="MKTSLTELDQRLLNDFQQDFPLSTTPYADIARSLGISEEQVLER
                     LAALKRAGAISRVGAVVRPNTVGVSTLAAMAVPPEELELVAAIVNGYAEVNHNYEREH
                     RLNLWFVASAPDVARLTAVLDEIAGRAGYEVLSFPLVEDYHIDLGFEMRWN"
     gene            complement(2529..3698)
                     /locus_tag="AC16FV_02830"
     CDS             complement(2529..3698)
                     /locus_tag="AC16FV_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR2849032.1"
                     /translation="MRVRTFLFPALGLLASACASPRGTGDLGVIIERAAGNVQVVETT
                     TRTRLAQVAGLGDLSHASVVYSRDARYAYIFGRDGGLTKVDLLRGRIEKRIVQAGNAI
                     GGAISQDGRLIAVSNYEPGGVKVFAADTLELLADIPAGSGDSQQRSKVVGLVDAPGDR
                     FVFSLYDAGEIWIADMHNPRQPQLRKFTNVGKQPYDGTVTPDGRYYIAGLFGEDGLAL
                     LDLWQPDKGVRRVLEGYGRGQEPLPVFKMPHMDNWAQANDIAYVPAVGRHEVLVIDQK
                     NWTEAGRIAVAGQPVFVVARPDGRQVWVNFAPPDNDTVQVIDTLSLAVVRTLKPGKAV
                     LHMEFTPRGEQVWMSVRDENRVDVYDTGTFERVSSLPADKPSGIFFSARAQRIGL"
     gene            complement(3688..4026)
                     /locus_tag="AC16FV_02835"
     CDS             complement(3688..4026)
                     /locus_tag="AC16FV_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2849033.1"
                     /translation="MRRPRHKTGIILAALGALWSGMTFADISTSRQAELMQLLTQDCG
                     SCHGLTRKGGLGPALTREALADKAPVMLREVILHGRPGTPMPPWKSFMNAQEADWLVQ
                     VLIEGKTDAR"
     gene            complement(4085..5785)
                     /locus_tag="AC16FV_02840"
     CDS             complement(4085..5785)
                     /locus_tag="AC16FV_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR2849034.1"
                     /translation="MTATALSFTSVMAAEEKPSGHPGTPETEMRYKGAPVPIKPEEAQ
                     EVVSPKAPPLTAAEFGRAKQIYFERCAGCHGVLRKGATGKPLTPDITLKKGTDYLKVF
                     INYGSPAGMPNWGTSGIMSDQDVEVMAKFLQHEPPTPPEFGMKEMKETWKVLVAPDKR
                     PKKKMNQINLNNLFSVTLRDSGEVALIDGDTKKIVNIVKTGYAVHISRLSSSGRYLYV
                     IGRDAKINLIDLWMDKPDNVAEIKVGLEARSVDTSKYKGYEDKYAIAGSYWPPQYTIM
                     KGDTLEPLKIVATRGFTVDTQEYHPEPRVASIVSSHFHPEFVVNVKETGHTLMVDYSD
                     INNLKVTDIATERFLHDGGWDSTKRYFLVAANQRNKIAVVDAKVGKLVKLVEVGKIPH
                     PGRGANFIDPKFGPVWATGHLGDDSIALIGTDPVKHKQNAWKMVRSLKGQGGGSLFIK
                     THPKSKNLWVDTALNPGADVSQSVAVYDINNLDKPYTVVKIAEMAGLGEGPKRVVQPE
                     YNKAGDEVWFSVWNGKTQESAIVVIDDKTRKLKTVIKDQRLITPTGKFNVYNTQHDVY
                     "
     gene            complement(5990..7699)
                     /locus_tag="AC16FV_02845"
     CDS             complement(5990..7699)
                     /locus_tag="AC16FV_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR2849035.1"
                     /translation="MKFPTVILRLLTLCLFMSVAAASHAQAIDELYPAVRTFFPQADR
                     FGELGGKLPAAPIYQGERLLGYAYLTGDIIRIPAYSGHPINTLVGFDLTGKIVGIRIV
                     EHQEPILVVGITEERLQQFARQYQEKSVFDDIVIGAGTPGQVAIDSISGATITVMVEN
                     ATLMRSVRRVAESRGLAPPALPVVTKTEVEPAKQTAPVSTTAPASSGASASRPTASAH
                     DAKAATAAKSKSASIVMTEEEPIWKGAWRKRTFQIAILAGGLTFLTLVLVFQDWLAKR
                     PRLLVYVRDGFLLYTVFFIGWYSLAQLSIVNVLTFAHSIMRGFQWESFLIDPMMFILW
                     AFVAMTLLLWGRGIYCGWLCPFGALQELTQQIARKFKIRQFEFSQMVHERLWALKYII
                     LLVLFGVSLQSLVQAEQMAEIEPFKTAITMRFQREWGYVLFAAGMILVSAVNRKFYCK
                     YLCPLGAALTIPGKFHIFDWLRRHRECGRPCQVCAVECEVQAIRKNGEINANECHYCL
                     DCQVTYWNDYKCPPEVERRKRRERSGRARELVRGMEPIIGPSGLFDQDQAGCANCPQR
                     DPK"
     gene            7847..8029
                     /locus_tag="AC16FV_02850"
     CDS             7847..8029
                     /locus_tag="AC16FV_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849036.1"
                     /translation="MEIIYFTLVAVGLYFASDWLLDRIEAARGSRFENRSVIFFVIIL
                     VLALVSFQILGRLIHS"
BASE COUNT         1562 a         2528 c         2451 g         1663 t
ORIGIN      
        1 ctcgcgcagg ccatcgatgc tgatgccgac gtagtcatag ccgatcgcgg cgatacgctc
       61 gatgttggat tcgtcgatca gcgtgccgtt ggtggagagg ccgacataga aacccatctg
      121 cttggcacgg cgagagatat cgaagacgtc cggccgcatc agcggttcgc cgccggagag
      181 gatcagcacc ggcacgcgga aacccttgag atcgtccatg accttgtaca cttccgcggt
      241 cgagagctcg ccggggaagt cggtgtcggc cgagatcgaa tagcaatgct tgcaggtgag
      301 attgcaacgg cgaatgagat tccagatcac caccggcccg ggcggatgtc gtttcggccc
      361 aagcggcgtg ggctgcttga tctcctgcat gtattgcgat atccggaaca ttgtcttgcc
      421 tcagtgttaa acgttaagtg gtaagtgtta agtagaagtt gcgcgaagcg ctcgattact
      481 aagcactgaa cactaaacac ttatacgtag cccggttttc ttgaggatgc gcgtgctgta
      541 cagcaccgtg tggccgcggt cggccgcgcc cagcagcgcg gcgatttgcg ctaccttggc
      601 cccgacctcg gcgcggtcat gcccgtgcac catggcgaac aggttgtacg gccagtcggg
      661 cagatgccgc gggcgatggt agcagtgact gacgaaatcg agtgcaccga ttttctgccc
      721 cagctcacgg atgcgctcct ccggcacgtc ccacaccgac atgccgttgg ccttgtaacc
      781 caggagatag tgattcggca ccgcgccgat gcggcggatc accccgctgt ccagcatgtg
      841 ctggagacga gccatcactt caccgacgct gatacccagt tgttccgcaa tggcatgata
      901 cggccggctc acacgcggca ggttttcctg cgtcgccacg atgatacggc ggtcgagcac
      961 atcaatctgt tccccttcgc ccctcgaggg gatatggaag ggatgagggg tgaaggattg
     1021 ttccccctca ccctggccct ctcccccaac tcgggggaga ggggattttt tctttaatgc
     1081 cttgcccata ttcatacctc gaacctcagg ccgacgaaaa attcctccag cttgggcatg
     1141 ttatagacct gataaccggt agccttttct atttcgccga tgacttcgtt gatacgctgc
     1201 ggcgtttcgg tcgccaatac gaaccacatg ttgaattcat gctcgcgcgc gtagttgtgc
     1261 gccacttcgg gaaaaccatt cacgatttca gccacgcgct caaaatcctg cggcgggatt
     1321 ttcatggccg cgagcgttaa tgccccgccc aagcgctcgg catgatacat cggagcgaaa
     1381 cggctgagcg cgcccttttc cagcatgtca ttgaggcgtc ggatcagatc gtcctcggtc
     1441 aggccgagtt ttgccgcggc ctcggcatag gggcgctcgc tgagcggaaa tccgccctgg
     1501 agattattga tgatatggcg gtcggtggtg tccattaccg tttctccacg ccggccgttc
     1561 tggcgtacac cgcgccacgt tgcttgaaac ggcggcggct gaacaacacc tcgtgcggca
     1621 ggctttgcag gccgcaatga ttgaccatcc attcgagatg cgtcagcacc tcctcgcggc
     1681 tcttgccgtg gatcatacag tagaggttgt agcgccactg cggcagatgc cgcggacgcc
     1741 ggtaacacag cgtgatgaaa tcgaaaccgg cgaggcagtg tccgagctcg gtcacctgtt
     1801 cgtccggcac gtcccacacc accatggcgt tggccgcata gccgagctca tgatgacgca
     1861 ccaccacgcc gaagcgacgg atcacaccgg cctccttgag ccacgccagg cgtgccacca
     1921 cctccatttc ggagatgcct agtccagccg caatctcggc gtacggccgc ggggtcagcg
     1981 gcagtccatg ctggatcgcc gccagcagac ggcggtcggc ttcgtccacc tggggccggc
     2041 gcagtgtcgc cgcggaatgt ttaatgacgt tcagttccat cgcatttcaa agcctaaatc
     2101 tatgtggtaa tcctccacga gcgggaagga caatacctcg taaccggcgc ggccagcgat
     2161 ttcatcaagc acagcagtca aacgcgcgac gtctggcgcg ctagccacga accacaggtt
     2221 cagccggtgc tcacgctcgt aattgtgatt cacttcggcg tagccgttga cgatagccgc
     2281 gaccaattca agttcttccg gcggcaccgc catggccgcc agggtgctga cgcccacggt
     2341 attgggccgc accaccgcgc ccacgcggct gatggcgccc gcgcgcttga gcgccgccag
     2401 tcgttccagg acctgctctt cgcttatccc cagactccgc gcgatatcgg cgtagggcgt
     2461 ggtggacagc gggaaatcct gctgaaaatc attcagcagg cgctggtcga gttcggtcag
     2521 acttgtcttc acaggcctat cctctgtgcc cgcgcggaga agaaaatccc gctcggcttg
     2581 tccgccggca gggaactcac gcgctcgaac gtaccggtgt cgtacacgtc cacgcggttt
     2641 tcgtcgcgca cggacatcca gacctgttcg ccgcgcggcg tgaactccat gtgcagcacc
     2701 gccttgccgg gcttgagcgt gcgcaccacc gcgagcgaca gcgtgtcgat gacctgcacc
     2761 gtgtcgttgt ccgggggcgc gaaattcacc cacacctggc gtccgtccgg gcgcgccacc
     2821 acgaacaccg gctggccggc cacggcgatg cggcccgctt ccgtccagtt cttctgatcg
     2881 atcaccagca cttcatgccg tcccacggcc gggacgtaag cgatgtcatt cgcttgcgcc
     2941 cagttatcca tgtgcggcat cttgaatacc ggcaatggtt cctgcccgcg gccgtagcct
     3001 tccagcacgc ggcgcacgcc tttgtccggc tgccacaggt cgagcaacgc gaggccatcc
     3061 tcgccgaaaa ggccggcgat gtagtagcgc ccgtcgggcg tcaccgtgcc gtcgtacggc
     3121 tgtttgccga cgttagtgaa tttgcgcaac tgcggctgac gcgggttgtg catgtccgcg
     3181 atccagatct cgccggcgtc gtacagggaa aacacgaagc ggtcgcccgg cgcgtccacc
     3241 aggcccacca ccttggaacg ctgctggctg tcgcccgatc ccgccgggat gtcggcgagc
     3301 aattcgagcg tgtcggcggc gaataccttc acgccgcccg gctcgtaatt ggaaaccgcg
     3361 atcaggcgcc cgtcctgcga gatggcgccg ccgatggcgt tgcccgcctg cacgatgcgc
     3421 ttctcgatgc gcccgcgcag caggtcaacc ttggtcagac cgccgtcgcg tccaaagata
     3481 taggcataac gcgcatcgcg cgagtaaaca actgaagcat gcgaaagatc gcccagtccc
     3541 gctacctgcg cgaggcgtgt gcgcgtcgtc gtttccacca cctgcacgtt gccggcggcg
     3601 cgctcgatga tgacgccgag atcgccggta ccacgcggag aggcgcatgc cgaagccaga
     3661 aggccgaggg caggaaagag aaaggttcta acgcgcatcg gtcttgccct cgatcaaaac
     3721 ctgcaccagc cagtccgctt cctgcgcatt catgaaactt ttccacggcg gcatcggtgt
     3781 tccgggacgg ccgtgcagaa tcacttcgcg caacatcacg ggggctttgt ccgccagcgc
     3841 ttcccgggtc agggccggac cgagcccgcc cttgcgcgtc agcccgtgac acgagccgca
     3901 atcctgcgtg aggagttgca tcaattcggc ctggcgtgac gtggagatat cggcaaaagt
     3961 catccctgac cacagcgcgc caagcgccgc gagaatgatg ccggtcttat gcctgggcct
     4021 gcgcatgcga acctgctctc ccgtaaagac ggggcgggat gactcccgcc ccttgctgcc
     4081 actactagta gacgtcgtgc tgcgtgttat acacgttgaa cttgcccgtc ggcgtgatca
     4141 ggcgctggtc cttgatcacg gtcttgagct tgcgcgtctt gtcgtcaatc acgacgatgg
     4201 ccgattcctg ggtcttgccg ttccacacgg agaaccagac ctcgtcgccg gccttgttgt
     4261 actcgggctg caccacgcgc ttcggaccct cgccgaggcc agccatttcg gcgatcttca
     4321 ccaccgtgta cggcttgtcg agattattga tgtcgtagac ggcgaccgac tggctcacgt
     4381 ctgcaccggg attgagcgcg gtgtccaccc acaggttttt ggacttcgga tgggtcttga
     4441 tgaacagcga cccgccgccc tggcccttga gcgagcgcac cattttccag gcgttttgct
     4501 tgtgtttcac cggatcggtg ccgatcaggg cgatggaatc gtcacccaga tggcccgtgg
     4561 cccagaccgg accgaacttc ggatcgatga aattggcgcc gcgtcctggg tgcgggatct
     4621 tgccgacttc aacgagtttc accagcttgc ctaccttggc gtccacgacg gcgatcttgt
     4681 tgcgctggtt ggcggccaca aggaagtagc gcttggtgga atcccagccg ccgtcatgca
     4741 ggaaacgctc ggtggcgatg tcggtgacct tgagattgtt gatgtcggaa taatccacca
     4801 tcagcgtgtg cccggtctcc ttcacgttca ccacgaactc ggggtggaag tgcgaggaaa
     4861 cgatcgaggc cacgcgcggc tcggggtgat actcctgcgt gtccaccgtg aagccgcggg
     4921 tcgcgacgat cttgagcggc tcgagcgtat cgcccttcat gatggtgtat tgcggcggcc
     4981 agtaggagcc ggcgatcgcg tacttgtcct cgtagccctt gtacttggag gtgtccaccg
     5041 aacgcgcctc gagaccgacc ttgatctcgg caacgttgtc cggcttgtcc atccacagat
     5101 cgatgaggtt gattttggcg tcgcgtccga tcacgtacag ataacgtccc gaggacgaca
     5161 gacgcgagat gtgaacggcg tagcccgtct tgacgatatt cacgatcttc ttggtgtcac
     5221 cgtcgatcag cgccacctcg ccgctgtcac gcaacgtcac cgagaagaga ttgttgaggt
     5281 tgatctggtt catcttcttc ttcgggcgct tgtcgggcgc cacgaggacc ttccaggtct
     5341 ccttcatttc cttcatgccg aactcgggtg gggtcggcgg ctcgtgctgc aggaacttcg
     5401 ccatgacttc gacgtcttga tcgctcatga tgccggaggt gccccagttg ggcatgcccg
     5461 cgggtgaacc gtaattgatg aacaccttga gataatccgt gcccttcttg agcgtgatgt
     5521 cgggcgtcag cggcttgccg gtcgcgccct tgcgcagcac gccgtgacag ccggcgcaac
     5581 gctcgaagta aatctgcttg gcgcggccga actcggccgc ggtgagtggc ggcgccttgg
     5641 ggctgaccac ttcctgcgcc tcttccggct tgatcggcac cggcgcgccc ttgtagcgca
     5701 tttctgtttc tggcgttccc ggatggccgg aaggtttttc ctccgctgcc atgacactcg
     5761 taaacgatag cgcggtcgct gtcatcagac cggcggcaaa tactgcgtgc ttgtatttca
     5821 tatcgcgttt cccccgcgtg agattaagaa atcatgagaa gtgggcactg tagaagtgcg
     5881 tgcaagcccg ctccttgacg tgagtcaaaa aagaccacaa tcgaagtgca agataggccc
     5941 gttcgacgga caagccggat gggcaagtga aatgcgggta attggcgatt tattttgggt
     6001 cgcgttgcgg gcaattggcg caacccgctt gatcttggtc aaacagcccg gagggtccga
     6061 tgatcggctc catgccgcgc accaattcgc gcgctcggcc acttctctcg cggcgtttgc
     6121 gccgttcgac ctccggcggg catttgtaat cgttccagta agtcacctgg cagtcgaggc
     6181 agtagtggca ctcgttggcg ttgatctcgc cgttcttgcg gatcgcctgc acctcgcatt
     6241 cgactgcgca cacctggcac ggacggccgc actcgcggtg ccgccgcagc cagtcgaaga
     6301 tgtggaactt gcccgggatg gtcagcgccg cgccgagtgg acagagatac ttgcagtaga
     6361 atttgcgatt gacggcggaa accagaatca ttccggcggc gaacaggaca tatccccatt
     6421 cgcgctgaaa gcgcatggtg atggcggtct tgaacggctc gatctcggcc atctgttcgg
     6481 cctgcaccag cgactggagc gagacgccga acaggaccag caggatgatg tatttgagcg
     6541 cccagagacg ttcatgcacc atctgggaaa actcgaactg gcgtatcttg aacttgcgcg
     6601 cgatttgctg cgtcagttcc tgcaacgcac cgaacggaca cagccagccg caataaatgc
     6661 cacgacccca gagcagcagc gtcatcgcca cgaacgccca caggatgaac atcatcggat
     6721 cgatcaaaaa actttcccac tgaaaaccgc gcatgatgga atgcgcgaag gtgagcacat
     6781 tgacgatgga gagctgcgcc agcgaatacc agccgatgaa aaaaaccgtg tacaacagga
     6841 aaccatcgcg cacgtaaacc agcaggcgcg gccgcttcgc caaccagtcc tgaaacacca
     6901 gcaccagcgt caggaacgtc aacccgcccg ccagtatggc aatctgaaaa gtgcgcttgc
     6961 gccaggcgcc cttccagatt ggttcctctt cagtcatgac gatggaagcg gattttgatt
     7021 tcgccgcagt cgctgccttg gcgtcatgcg cgctggcggt cggtcgggaa gcggacgcac
     7081 ccgaagaggc cggagcagtt gttgataccg gtgctgtctg cttcgccggt tcgacctccg
     7141 ttttcgtgac cacgggtaat gccggcggcg ccaagccgcg cgattcggcc acgcggcgga
     7201 cggagcgcat cagggtggcg ttctcgacca tcacggtgat ggtggcgccg gagatactgt
     7261 cgatggccac ttggcccggc gtgccggcgc cgatgacgat gtcatcgaac acggattttt
     7321 cctgatactg cctggcgaat tgctgcaagc gctcttcggt gatgccgacc accagaatcg
     7381 gctcctggtg ttcgacgatg cggatgccga cgattttccc cgtgagatcg aacccgacca
     7441 gggtgttaat gggatgaccg gaataggccg ggatgcggat gatgtcgccg gtcagatagg
     7501 cgtacccgag cagacgctcg ccctgataaa tgggggccgc cggcagcttg cccccgagct
     7561 cgccgaaacg gtccgcctgc gggaagaacg tccttaccgc cgggtaaagt tcgtcaatgg
     7621 cctgggcatg agacgcggcc gccacgctca tgaacagaca cagcgtcaac aggcggagaa
     7681 tgacggtcgg gaatttcacg aggtactcat gcgggcggga aggcgtccct gaataggctg
     7741 taaccagcct gatacaggca gattatagga gcattgattt ctgtcaattg ccggctcgcg
     7801 ccacccgcta gaatcgcgtt acatccggtc aaggggcgca atttcgatgg aaataatcta
     7861 tttcacactg gtcgccgtcg ggctttattt cgcctccgac tggctgctgg accgcatcga
     7921 ggccgcgcgc ggcagccgtt tcgagaaccg ctcggtcatc ttcttcgtca tcatcctggt
     7981 gctcgcgctc gtctcgttcc agattctcgg tcgcctcata cattcgtaaa cagcgcgaca
     8041 atacaaaagg cgatggattt tcccttcgcc ttttgcccac tcccgtaaaa atttccgcta
     8101 gaaccggata tgcaccgagg cgttcaagct cgaaggaatt accgtgaagc gtctcagggt
     8161 tttacgctga gcgatcgctt cggccacatg gcgcggtaac aaac
//
LOCUS       DBNGZE010000112         2505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1682918, whole genome shotgun sequence.
ACCESSION   DBNGZE010000112 DBNGZE010000000
VERSION     DBNGZE010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1682918"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..689)
                     /locus_tag="AC16FV_02855"
     CDS             complement(72..689)
                     /locus_tag="AC16FV_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HIR2849037.1"
                     /translation="MDHAEIPAFALTLWPTKHISELVVRVIQAALSLKYQEDNLMLNK
                     KLMVVAVAGLFSTGAMAAGENNTGTCGWGAKLFDGQRGIAPQVLAATTNGTSGNQTFG
                     ISSGTSGCTQDGVVKSNWKTAMFIDGNKEKVARDMSTGSGESLESLAKLIGVQDQHKA
                     AFFRATKENFAKIFSSDNATTDQVLASLKQVLASDSELAQYSLSI"
     gene            782..1174
                     /locus_tag="AC16FV_02860"
     CDS             782..1174
                     /locus_tag="AC16FV_02860"
                     /inference="COORDINATES: protein motif:HMM:NF022841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3192 domain-containing protein"
                     /protein_id="HIR2849038.1"
                     /translation="MKKLIVSLTLAVSGCSNIYLDTSDLLRAQNTGNLRKLSVGLKKE
                     VAVEIMGTEPSKGVFMWIDNPYRSETLTGKDGKNYEVLYYYTDLKQRDDKITDDELTP
                     LVFQDGKLIGWGYPFLDQKAPPKPAYLR"
     gene            complement(1188..2075)
                     /locus_tag="AC16FV_02865"
     CDS             complement(1188..2075)
                     /locus_tag="AC16FV_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR2849039.1"
                     /translation="MDSLILWIVVFTALGGVLSVFAAAAFLLVPEETRTRLLSPMVSF
                     AIGALLGASFLALLPHAFETPGVDVHRVALTVLCGILVFFLLEKMVVWRHCHTHDCDV
                     HGGTNVGRSQHPASRDTSTSLYSAGTTGMPVAAEYRALDVDKARHAATGNLILIGDGI
                     HNMVDGVLIAAAFLTDIHLGIITSIAVIAHEVPQELGDFAILLHSGFSRSRALLYNAL
                     TGLTTIIGGVVAYFSLSLANQIVPYVLAIAASSFIYIAVADLIPGLHKRPEFSATVQQ
                     IVLIALGVVVIFLAHSTLH"
     gene            complement(2114..>2505)
                     /locus_tag="AC16FV_02870"
     CDS             complement(2114..>2505)
                     /locus_tag="AC16FV_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR2849040.1"
                     /translation="ALHAGWRGLAAGVIEEGLRKIQVPPANLLAWLGPAIGPSAYEVG
                     DDVRRAFVALDKDMAESFTPVPNRAGHWLADLYDIARRRLRSQGVHAVYGGLRCTLRE
                     RNLFYSHRRDGTCGRMASLIWLDKHAR"
BASE COUNT          581 a          714 c          750 g          460 t
ORIGIN      
        1 aatcacggga gatataaaaa cggcacccag cttacaacag ctgggtgccg cggtgatatg
       61 acagcttggt gttagatcga cagagaatac tgcgcgagtt cgctgtcaga ggccaatacc
      121 tgtttcaggg aagccaatac ctgatcggtg gttgcattgt cggaagagaa gatcttcgca
      181 aaattttcct tggtggcgcg gaagaatgcc gccttgtgct gatcctgaac gccgatcagc
      241 ttggccagag attccaggga ctcaccgctg ccggtggaca tgtcacgcgc caccttttcc
      301 ttgttgccgt cgatgaacat cgcggtcttc cagttggact tcaccacgcc atcctgcgtg
      361 cagcccgagg tgccggacga aatgccgaaa gtctggttgc cggacgttcc gttcgtggtc
      421 gcggcgagga cctgaggtgc gatcccgcgt tggccgtcga acagcttggc gccccaaccg
      481 caggttcctg tattattctc gcccgccgcc atggcaccgg ttgagaacaa tcccgctaca
      541 gcgacaacca tgagcttctt gttcaacatt aagttatcct cctgatattt aagggataaa
      601 gcagcctgaa ttactcgaac aaccaattca cttatatgct tggtcggcca aagtgtcaag
      661 gcaaacgcgg gaatttcagc atggtccatg acgcctttga tcgggattta tttcaaacag
      721 atgaaatccg tgctggcaac atttcaccga aacggcgtat tcaataacgg caggagaaaa
      781 tatgaaaaaa cttattgtct cgttgacatt ggctgtttcc gggtgcagca acatctatct
      841 cgacaccagc gatctcttga gggcgcagaa taccgggaat ctcagaaaac tgagcgttgg
      901 tctgaaaaag gaggtggccg tggagatcat ggggaccgaa ccctccaaag gcgtattcat
      961 gtggatcgac aatccctacc ggagtgaaac cttgacgggc aaggatggga aaaattatga
     1021 agtcctgtac tactacaccg acctgaagca gcgcgacgac aagatcaccg acgatgaact
     1081 gacgccgctc gtgttccagg acggaaagct gatcggttgg ggctacccct tcctggacca
     1141 gaaagcgccg cccaagccgg cgtacctgcg ctaagccgcc ggaagagtca gtgcagtgtg
     1201 gaatgggcca ggaaaatcac gaccacgccg agcgcgatca gcacgatctg ctgcaccgtg
     1261 gcgctgaact cgggacgctt gtgcagacca ggaatcaggt cggccacggc gatgtagatg
     1321 aagctcgatg cggcgatggc cagcacatac ggcactatct gattggcgag cgacagggag
     1381 aaataggcta caacaccgcc gataatcgtg gtcagcccgg tgagggcgtt gtacagcaag
     1441 gcccgtgaac ggctgaagcc gctgtgcagc agaatggcga agtcaccgag ctcctgcggg
     1501 acttcgtggg cgatcacggc gatgctggtg atgatgccaa ggtggatgtc ggtcaggaac
     1561 gccgccgcga tcagtactcc gtccaccata ttatggatac cgtcgccgat caggatcagg
     1621 ttgccggtcg ccgcgtgacg cgccttgtcc acgtccaacg cacggtactc ggccgcgacc
     1681 ggcattcctg tcgtccccgc gctgtacagg gatgtactag tgtcgcgaga agcaggatgc
     1741 tgtgagcgac cgacattagt gcctccatgc acgtcacaat catgcgtgtg acagtgacgc
     1801 cagaccacca ttttctccag caggaagaaa accagaatgc cgcacagcac ggtaagcgcg
     1861 acccgatgca cgtccacccc cggtgtctcg aaggcatgcg gcaggagggc aaggaaggat
     1921 gccccgagca acgcgccgat ggcgaagctc accatgggcg acagcaggcg cgtgcgcgtt
     1981 tcctccggca ccagcaggaa ggctgccgcc gcgaacacgc ttagcacgcc gccgagggcg
     2041 gtaaaaacga cgatccagag aatcagggaa tccatttttg ggagcggccc ggcgagaaag
     2101 ccggcatcat aacttaacgc gcgtgtttat ccagccagat gagcgaagcc atgcggccgc
     2161 aggtgccgtc gcgccggtgg gaatagaaaa gattacgctc gcgcagcgtg cagcgcaggc
     2221 cgccataaac cgcgtgcacg ccctgcgaac gcagacgccg gcgcgcgatg tcgtaaagat
     2281 cggccagcca gtgtcccgcg cggttgggta cgggggtgaa cgattcggcc atgtccttgt
     2341 ccagcgcgac aaacgcccgg cgcacgtcat ctcccacctc gtaggccgac gggccgatgg
     2401 ccggaccgag ccaggccagc agattggccg gggggacttg aattttgcgc aagccttctt
     2461 ctatgacgcc ggcggccagg ccgcgccagc cggcatgcaa cgctg
//
LOCUS       DBNGZE010000113         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_779918, whole genome shotgun sequence.
ACCESSION   DBNGZE010000113 DBNGZE010000000
VERSION     DBNGZE010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_779918"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..320)
                     /locus_tag="AC16FV_02875"
     CDS             complement(117..320)
                     /locus_tag="AC16FV_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2849041.1"
                     /translation="MQTGTVKWFNNAKGYGFITPSDGSEDVFVHFSTIEGDGYKSLNE
                     GQKVEFEHTQGPKGKQATRVIPR"
     gene            complement(508..879)
                     /locus_tag="AC16FV_02880"
     CDS             complement(508..879)
                     /locus_tag="AC16FV_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR2849042.1"
                     /translation="MHPKATQSPPYSLDRASLEREVRVDTYRASGPGGQHVNRTESAV
                     RLTHAPSGVVVTAMESRSQIRNREIAFERLMHKLRALNRVPKPRKATRVSRAVKERRL
                     AAKKHRASIKRGRSRGREEEM"
     gene            975..2099
                     /locus_tag="AC16FV_02885"
     CDS             975..2099
                     /locus_tag="AC16FV_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR2849043.1"
                     /translation="MIVILKPDMGEKTPEYRQILDFLAHKPNIKTRVHQEIGAQQTLT
                     EIYLIGDTASLDKSEIESLPGVERVVRISEEYRVLGRHRDDRRPTGFEYNGVKFNQDN
                     LNVFAGLCAVDNPEHVEMMLKALRDNGQVCTRMGAYKPRTNPYSFQGHGKECLPWVFE
                     LAGRYGIKVIAMEITHDSHLNEIQEALHKTGRPTGVMLQIGTRNTQNFELLKIVGRQR
                     ELPVLLKRGFGITLEESLNAAEYLASEGNRNIVFGLRGMKTNMGDPHRNFVDFAHVPV
                     IKRLTRMPVCIDPSHSVGTRDAAPDGMLDIAHVTAQGVIAGANMILVDFHPVPSKALV
                     DGPQALLLEELPYFLEDVAIAREAYEKRRVLVQQFRKKGK"
BASE COUNT          495 a          662 c          650 g          458 t
ORIGIN      
        1 catgcgacat gttcgatggc gatgaaaatc aactcagtga cgggtgcaat aaaaagcccc
       61 gtgcatggac ggggccggaa aacagatgat atttatagat gtctgttcgt aatctttcag
      121 cgggggatga cgcgcgtggc ctgcttgccc ttcggtcctt gcgtgtgttc aaactcgacc
      181 ttttgcccct cgttcagact tttgtatcca tctccttcga tggtggaaaa atgcacaaac
      241 acatcctcac tgccgtcgct cggggtgata aaaccataac ccttcgcgtt gttgaaccac
      301 ttcacggtac cggtctgcat acatccccct ttcttatatg gaacctgaaa ccgcccgccc
      361 tgcggtcggg actggcgcga aaacatccta cgtcaactcg ccggacaaaa caattctgcc
      421 gggtaataca ggcatagacg ggttctacgg cggggacatt tgagcaaact cttatatagc
      481 caagacgagc gagcgcagcg agcagggcta catttcctcc tcgcggccgc gagaccgccc
      541 gcgtttaata gaggcgcgat gcttcttggc ggccagccgg cgctccttca cggcccggct
      601 gacgcgggtg gccttgcgcg gcttgggcac gcggtttagt gcacgcaatt tgtgcataag
      661 ccgctcgaaa gcaatttcgc ggttgcggat ctgggaacgg ctctccatgg ccgtcaccac
      721 caccccggaa ggcgcgtggg tcaggcgcac cgccgactcg gtgcggttga catgttgccc
      781 gccgggaccg gaggcgcgat aggtatccac gcgcacctcg cgttccaggc tggcacgatc
      841 cagcgaatat ggcggagatt gcgtggcttt cggatgcatg cgggttaatt tccggactga
      901 tttttctgac aatcgcggct tagttggtaa aatacgcgcc tgtgagacat accgaataat
      961 aaaggaaaag tggcatgatc gttatcctca agcccgacat gggcgagaag actccggaat
     1021 accggcagat tctcgatttt ctggcgcaca agccgaacat caaaacgcgc gtccaccagg
     1081 aaatcggcgc gcagcagacg ctcacggaaa tctatttgat cggcgacacc gcctcgctcg
     1141 acaaatccga gatcgagagc ctgcccggcg tggaacgcgt ggtgcgtatc tcggaggaat
     1201 accgcgtgct ggggcgtcat cgcgacgatc gtcgtccgac gggtttcgaa tacaacggcg
     1261 tcaaattcaa ccaggacaat ctcaatgtct tcgccggcct gtgcgccgtg gacaacccgg
     1321 agcatgtcga gatgatgctg aaggcgctgc gcgacaacgg ccaggtctgc acgcgcatgg
     1381 gcgcttacaa gccgcgcacc aatccatatt cattccaggg ccatggcaag gagtgcctgc
     1441 cgtgggtgtt cgagctggcc ggccggtacg gcatcaaggt gatcgccatg gagatcaccc
     1501 acgactcgca cctgaacgaa atccaggagg cactgcacaa gaccggcagg cccaccggcg
     1561 tgatgctgca gatcggcacg cgcaataccc agaacttcga gttgctcaag atcgtcggac
     1621 gccagcgcga gttgccggtg ctgctcaaac gcggcttcgg catcacgctg gaggagtcgc
     1681 tgaacgcggc cgagtatctg gcgagcgaag gcaaccgcaa catcgtcttc ggcctgcgcg
     1741 gcatgaagac caacatgggc gacccgcacc gcaacttcgt cgatttcgcc cacgtgccgg
     1801 tgatcaagcg cctgacgcgc atgccagtgt gcatcgatcc ttcgcactcc gtcggcacgc
     1861 gcgacgcggc gcccgatggc atgctcgata tcgcgcacgt caccgcgcag ggcgtgatcg
     1921 ccggcgccaa catgattctg gtggattttc accccgttcc gtccaaggcc ctggtggacg
     1981 gcccgcaggc gctgctgctg gaagaactgc cctattttct ggaagacgtg gcgatcgcgc
     2041 gcgaggccta cgaaaaacgc cgggtgctag tgcagcagtt ccggaaaaaa ggaaaataga
     2101 caggatttac acgattaaca agattagaaa tattttttac tatcctgtta atcctgaaaa
     2161 atcttgttaa tcctgtctat tgtttgtttc ttgttgtcag ctcagcgcgc gatgccgtaa
     2221 acgacgtcga agaccacgcg cgttttcccg tccatcagca cgata
//
LOCUS       DBNGZE010000114        10674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1543552, whole genome shotgun sequence.
ACCESSION   DBNGZE010000114 DBNGZE010000000
VERSION     DBNGZE010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1543552"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="AC16FV_02890"
     CDS             complement(<1..550)
                     /locus_tag="AC16FV_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HIR2849044.1"
                     /translation="MSRSESSPALVLNHGLTFAELFTPEGLQRIDGLFLDRLRERDAR
                     AHTALLAWRAGKETLTPLQVSELLLACGPVLDEFIAHLFNIQDALKATRRGTLAHDPV
                     FHFKKLFVQRRARRRLLKKEEFENFAELDAWLTQALKQAGLKTSDRELAIAQYGEQLL
                     KDEKAHAESIEKLTRWCLRALTV"
     gene            complement(692..892)
                     /locus_tag="AC16FV_02895"
     CDS             complement(692..892)
                     /locus_tag="AC16FV_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR2849045.1"
                     /translation="MEKNIGNVDKALRIVIGIALLSLLFILEGNARWWGLIGIGPILT
                     VILGWCPAYTLIGVSTCKTKQS"
     gene            complement(962..1435)
                     /gene="moaC"
                     /locus_tag="AC16FV_02900"
     CDS             complement(962..1435)
                     /gene="moaC"
                     /locus_tag="AC16FV_02900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR2849046.1"
                     /translation="MSKLTHLDESGAARMVDVGGKDVTAREAVAEGLIRMQPETLRLI
                     VEGGHKKGDVLAVARVAGIMAAKKTAELIPLCHAIPLTAVDIELTADEKRSAVYCRGT
                     VRTRDRTGVEMEALTAVQIALLTIYDMCKGVDRGMTLTDIGLVSKSGGRSGDWRR"
     gene            complement(1624..2163)
                     /gene="moaB"
                     /locus_tag="AC16FV_02905"
     CDS             complement(1624..2163)
                     /gene="moaB"
                     /locus_tag="AC16FV_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HIR2849047.1"
                     /translation="MSKKAKGPFISLNIAVLTVSDTRTRENDTSGDLIQERLESAGHK
                     VAARVILPDDVEKLRAQLRAWMANKSVDAVISTGGTGLTKRDVTPEAFEPLFTKTSPG
                     FGELFRWLSYEEIGTSTIESRAFAGVADDTLVFCLPGSTGACRTGMDKIILPQLNATT
                     GPCNLTEMLPRIRHDPAPK"
     gene            complement(2171..2875)
                     /locus_tag="AC16FV_02910"
     CDS             complement(2171..2875)
                     /locus_tag="AC16FV_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2849048.1"
                     /translation="MNRSPDVARELRRAYLFADMTEPHLQMLVNGMHDIHLDPGKALF
                     RHGQPAERFYFLREGLIKLFRLSPEGDEKIIEIMRPGETFAEAVMFMGSQGRYPVNAE
                     AINESRLYAFEQKTFLSLLRESSDATYGLLGSMSRRLHMLVNQIESLTLQNATYRLVA
                     YLLEEIPRDVRTSPEVQLTTPKGVIASRLAIQPETLSRILAKLRQGGLIEVHGNHITI
                     RDVQALRNLVHLPPQE"
     gene            2989..3234
                     /locus_tag="AC16FV_02915"
     CDS             2989..3234
                     /locus_tag="AC16FV_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2849049.1"
                     /translation="MKVQLLVSETCVPCDQAEKVWRQVADERAFDFSVVNLGDPLGRQ
                     LADDLQLRTIPAVVIDGVLVAIGVQSLEEARALISRT"
     gene            3312..4511
                     /locus_tag="AC16FV_02920"
     CDS             3312..4511
                     /locus_tag="AC16FV_02920"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR2849050.1"
                     /translation="MLSLEEAQAAILAEVTPSTATQTVPLAQAHGRFTAQELCAQVDN
                     PAFDNSAMDGYAVRAADLVAAGFVLPLRGECRCGDAPAKLTPATAMRIFTGAPMPEGA
                     DTIVIQEDVKLEGDKVIFPKSAQPGSHLRRRGEDFRRGDVLYRPGRKLSAYDLALLSA
                     AGVAEMPVYSRARVLVAATGDELVSPGTPLRPGQIYESNRLATLLLLRELGMDAVDGG
                     TVRDDPDALRAWLKSSTDYDFVITSGGASVGDHDLVKQVFAEIGAIKFWKAKIKPGKP
                     IAFGRVGMRTHFFALPGNPVSSLVTFKLFVEPALIAWHHGVSHMPQLAATATNDFRRH
                     PGRTEFVRARLATEDGKLLATALRGQGSHMLGPLRETNGFIRVEADSAGFQQGETVAA
                     IPLNTDF"
     gene            4517..5107
                     /gene="mobA"
                     /locus_tag="AC16FV_02925"
     CDS             4517..5107
                     /gene="mobA"
                     /locus_tag="AC16FV_02925"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR2849051.1"
                     /translation="MNIATHDITGVILAGGRGSRLGGVDKGLVRLRGRPLIEYAINAL
                     RPQVGSLLISANRNRDVYASYGYHVIADVMGNYDGPLAGMLSAMRHADTPYIVTAPCD
                     VPAPPVDLVERLAGAMSMTQREACIASCEGQTQPVFALVRCALADRLQEYLAAGERGV
                     ENWMRQQQAAIVDFPEHDAFVNINTEEDLRRLEAVA"
     gene            complement(5119..6519)
                     /locus_tag="AC16FV_02930"
     CDS             complement(5119..6519)
                     /locus_tag="AC16FV_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2849052.1"
                     /translation="MRSIQKHLGVSLAVSLVVVFFLQWLVVSVTLRHLSESGFTVHME
                     HDIENLLTAISFDAAGRMQLAPDRIEKVYQQPFSGSYYRIAAGDQTIRSRSLWDQDLP
                     AVSDATGGILTQRLPGPQSQSLIMYTRNFEWRGRRLVISVAEDLTPLENDMREFRNRY
                     ALVSLAALALLLALQAWLVRRGLRPLHRTRAELKQLEQGEVSRLSEAVPAELSPLVKE
                     LNRLLTAMTQRLTRSRQALGNLAHALKGPLTLLTHQVAEEKTDAAAPRQAMAEQLQTM
                     KYLIERELKRARLSGATLPGRRFDFATEMPALIETLKALYREKPLEISMRMPAAVSFP
                     ADREDMLELLGNLLDNACKWGRSEVYVEVTFTSTTDSPSPLAGEGWGEGEKALIEIRV
                     HDDGPGCPDESLASLAQRGTRLDESMPGQGLGLAIAMQTASDYGGRMGFGRSEILGGF
                     LATVQLPVERRTTTDA"
     gene            complement(6519..7181)
                     /locus_tag="AC16FV_02935"
     CDS             complement(6519..7181)
                     /locus_tag="AC16FV_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2849053.1"
                     /translation="MRLLVVEDDAALCASLEQALEQAGYAVDTTDNGEDAEHLGDTEP
                     YDAVVLDLGLPRRPGLEVLRHWRTRGKTMPVIILTARDTWQEKVDGFKAGADDYLAKP
                     FHFEELVARVNAIIRRRHGEPKGGIQAADLVLDEERQTVTYHGKTIELTGTEFRLLRY
                     FMLHPGKILSKTELTGHVYDFDSERDSNVIEVYIRRLREKLDPDVIHTRRGQGYIFGA
                     NS"
     gene            complement(7183..7488)
                     /locus_tag="AC16FV_02940"
     CDS             complement(7183..7488)
                     /locus_tag="AC16FV_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR2849054.1"
                     /translation="MKTLPKTLLTAAVLLTFAVPGFASESQDEAKKLREAGDILPLEQ
                     ILEKAKQKQPGRVVETELERKSRRHVYEVKIVDEKGVVHELEYNAKNGELLKAEKEK"
     gene            complement(7518..8174)
                     /locus_tag="AC16FV_02945"
     CDS             complement(7518..8174)
                     /locus_tag="AC16FV_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019953307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR2849055.1"
                     /translation="MNPPETPSTPAAVKVWDPLVRLFHWSLVATFIIAWLTADEESRL
                     HELAGYAVIGLVLVRIVWGFIGTRYARFSDFVYRPSTVLGYAREMLIGKPGRYLGHNP
                     IGGVMVIALLVSLLATGVTGLALQGAEEGTGPFASLSTGAAVAMPSVIAKAVADDDEG
                     GNGGEENEGHEELREELHEFFANLTLLLVALHIAGVIIGSLVHRENLVRAMFTGRKRV
                     "
     gene            8335..8733
                     /locus_tag="AC16FV_02950"
     CDS             8335..8733
                     /locus_tag="AC16FV_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR2849056.1"
                     /translation="MPELSEKQLQDFTQRLRNRREELRDIIREELASTQREDYTELAG
                     MVHDAGDESFAELLRGINLTTRARELEEIQDVEAALERIKNGIYGSCVDCNDSIALER
                     LDAYPTAKRCINCQSRHENRRGGRDATPSL"
     gene            8795..8870
                     /locus_tag="AC16FV_02955"
     ncRNA           8795..8870
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_02955"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            complement(8948..10111)
                     /locus_tag="AC16FV_02960"
     CDS             complement(8948..10111)
                     /locus_tag="AC16FV_02960"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR2849057.1"
                     /translation="MIRTPLPLLTLRGRELLPVIQGGMGVGISGHRLAGAVAKEGAVG
                     TIASVDLRRLYPDIMQRLRKCHDPKVHSAANIEALDREIRAAHEIAPDGFVAVNVMRA
                     LSNYAELVVQACKSGANAIISGAGLPFDLPELTEKFKNVALIPILSEERGVRAVLKKW
                     MRKGRLPDAIVIEHPRYAGGHLGATRMEEVNDPKFDFGRVLEEIRRVFQELGIALGSI
                     PLIPAGGINSFQKLKELFDMGAAGAQIGTPFAVTEECDAHPNFKKVLAEAKPEEIVTF
                     MSAAGLPARAVLTPWLKNYLAKEEKLRAKASPECSQCPSRLECLAHCGFKDGNPHAGQ
                     FCIETQLAAAQRGNIQQGLFFRGSESLPFGAEIRKVRDLLEVLLTGRPLTATL"
     gene            complement(10208..>10674)
                     /locus_tag="AC16FV_02965"
     CDS             complement(10208..>10674)
                     /locus_tag="AC16FV_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR2849058.1"
                     /translation="LPVVGLALRADVRASARGRETRITTDLHPMWWLKYIHVTCVVLS
                     GTGFFIRGIWMIRESPRLHEKWVRVLPHIIDTTLLVSAILLAWQIRQYPFVHGWLTAK
                     VLALVAYIVVGAVGLKYGRTKKIRITAWLGAIAIFLYIVLVALTRQVLPFVA"
BASE COUNT         1996 a         3256 c         3270 g         2152 t
ORIGIN      
        1 gcacggtgag cgcgcgcagg caccagcgcg tgagtttctc gatggactcg gcatgggcct
       61 tttcgtcctt gagtaattgt tcgccgtatt gcgcgatcgc cagttcgcga tcgctggttt
      121 tcagaccagc ctgtttcaaa gcctgcgtca gccaggcgtc cagctcggcg aagttttcga
      181 attcctcttt tttcagcagc cgccggcgcg cacggcgctg cacgaacagc ttcttgaaat
      241 gaaataccgg atcgtgcgcc agcgtgccgc ggcgcgtagc tttcagcgca tcctggatgt
      301 tgaagagatg ggcgatgaac tcgtccagca ccggcccgca ggcgagcagc agttcgctca
      361 cctggagcgg cgtgagggtt tccttgccgg cgcgccacgc gaggagggcg gtgtgcgccc
      421 tggcgtcgcg ctcgcgcagg cggtccagga acaggccgtc gattctttgc aggccttccg
      481 gcgtgaacag ttcggcgaaa gtcaggccgt gattcagcac cagggccggc gacgattcag
      541 atcgggacat gaaggcttcg aattaaatct ttttggctgg ttcggcgagg gcgtattgta
      601 attcctcgcg cgccggaatg catatgcgga aaaacaagaa agccgcgtga ctcacgcggc
      661 tttcttgcgc aatgtcaggc ctgtgcggcg gtcaagactg tttggtcttg caagtactga
      721 cgccgatcag ggtataggcc ggacaccaac ctaatatcac cgtcagtatc gggccgatgc
      781 cgatcaatcc ccaccagcgc gcgttacctt ccagaatgaa cagcagactc agcagcgcaa
      841 ttccaatgac gatgcgcaat gccttgtcca cgttgccaat gttcttttcc atagaagacc
      901 tccttggcgc gagagtattg aggtttccag catagcgccg gtgcgcccgg gcaacaacgg
      961 ctcaacgccg ccaatcgccg gaacgaccgc cggatttgga aaccaggcct atatcggtaa
     1021 gcgtcatgcc gcggtcgacg cccttgcaca tgtcatagat ggtcagtagc gcgatctgca
     1081 ccgccgtgag cgcctccatc tccacgccgg tccggtctcg ggtgcgcacg gtgccgcgac
     1141 aatacaccgc ggaccgtttt tcatcggccg tcagctcgat gtccacggcg gtaaggggaa
     1201 tggcgtggca cagcgggatg agttcggcgg tttttttcgc cgccatgatg ccggccacgc
     1261 gcgcgaccgc gagcacgtcg ccttttttat gcccgccttc gacgatgagc cggagcgttt
     1321 ccggttgcat gcggatcagt ccttccgcca cggcctcacg cgcggtgacg tccttcccgc
     1381 ccacgtccac catgcgcgct gcgccggatt cgtcgagatg tgtcagcttg ctcatagata
     1441 caggcaattc cttatatgaa ttttgccgca aagacggtcg ctcccgcgca tccctgcgcc
     1501 cgcgacattc gtacttcctg tacagcacaa taagcgcaaa gattttcggg ttttgttctc
     1561 gcaagtcttt ctttgcgtcc tctgcgttct ctgcggcaga tgatcatact aaatctctct
     1621 tccttacttg ggagctggat cgtgacggat gcgcggcagc atctcggtca ggttgcaggg
     1681 cccggtggtc gcgttcagtt gcggcaggat gatcttgtcc atgccggtgc ggcaggcgcc
     1741 ggtcgaaccc ggcaggcaga acacgagcgt gtcgtcggcg acgccggcga aggcacgcga
     1801 ctcgatggtg gaagtgccga tctcctcgta ggacagccaa cggaacagct cgccgaagcc
     1861 gggactggtt ttggtgaaca gcggctcgaa tgcttcggga gtgacatcgc gtttcgtcag
     1921 tcccgtgccg ccggtcgaaa tgaccgcatc cacgctcttg ttcgccatcc aggcgcgcaa
     1981 ctgtgcacgc agtttctcga cgtcatccgg caagatgacg cgcgccgcca ccttgtgccc
     2041 ggcgctttcc agtcgttcct ggatgagatc gccggaagta tcgttttcgc gcgtgcgcgt
     2101 gtccgatacc gtcagcaccg cgatgttcag ggaaatgaaa gggcctttgg cttttttgct
     2161 catggttatt ttattcctgc ggcggcaggt gcacgagatt gcgcagcgcc tgcacgtcgc
     2221 gaatggtgat gtgattgccg tgcacttcaa tcaatccgcc ctggcgcagc ttggcgagga
     2281 tgcgtgacag cgtttccggc tggatcgcga ggcgcgaggc gataacgccc ttgggcgtgg
     2341 tgagctgcac ctcgggcgag gtcctgacgt cgcgcgggat ttcctcgagc aggtacgcca
     2401 ccaggcgata ggtcgcgttt tgcagcgtga gactttcgat ctggttcacc agcatgtgca
     2461 gccgccggct catggaaccc agcaggccgt aggtggcgtc gctcgattcg cgcaacagac
     2521 tgagaaaggt cttttgctcg aaggcgtaga gccggctttc gttgatggcc tcggcgttga
     2581 ccggataacg cccctgggag cccatgaaca tgaccgcctc ggcgaaggtc tcgcccggac
     2641 gcatgatctc gatgattttt tcgtcgccct ccggcgacag gcggaagagc ttgatcaatc
     2701 cttcgcgcag aaaataaaag cgctccgccg gctgcccgtg ccggaacagc gccttgccgg
     2761 gatcgagatg gatatcgtgc atgccgttga cgagcatctg cagatgcggt tccgtcatgt
     2821 ccgcgaacag gtaagcgcgg cgcagctcgc gcgcgacatc gggagatcgg ttcatgccgt
     2881 aatcataacc tcagcgaggc ccggttgacc aaggtcaatg cccttgccgg cgcggcgttt
     2941 cagagtaaca catgctcccg gcgccggtcc ggggcaacag gcggaggaat gaaggtccag
     3001 ctactggttt cggaaacgtg tgtcccttgc gatcaggcgg aaaaggtctg gcgccaggtg
     3061 gcggacgagc gcgcgttcga cttttccgtg gtcaatctgg gcgatccgct gggccggcag
     3121 cttgccgatg atttgcagct cagaaccatc ccggccgtgg tgattgacgg tgtgttggtc
     3181 gccatcggcg tgcagagcct cgaagaagca cgtgcgctga tctcacggac atagcttcaa
     3241 cgatcctggt tctccccata tatccctgac gcggtctagg ccttggtcct gatttgcgct
     3301 accgtttcgg gatgctgagt ctcgaagaag cccaagccgc cattctcgcg gaagtaacgc
     3361 catccaccgc gacacagacg gtgccgttgg cgcaggcgca tggccgtttc accgcgcagg
     3421 aactttgcgc gcaggtagac aaccccgctt tcgataattc cgccatggat ggttacgccg
     3481 tgcgcgccgc cgatctggtg gccgccggtt ttgtcttgcc attgcgcggc gaatgccgct
     3541 gcggcgatgc gccggcgaag ctcacccccg caacggccat gcgcattttc acgggcgcgc
     3601 ccatgcccga gggcgccgac accatcgtga tccaggagga tgtgaagctc gagggagaca
     3661 aggtcatttt ccccaagtcg gcgcaaccgg gcagccacct gcgccgtcgt ggcgaggatt
     3721 ttcggcgcgg tgacgtgttg tatcgccccg ggcgcaaact ctccgcttac gatctggcgc
     3781 tgttgtccgc cgctggcgtg gcggaaatgc cggtttactc acgggcacgc gtgctggtgg
     3841 ccgccaccgg cgatgagctg gtatcgcccg gcacgccgct gcggcccggg cagatatacg
     3901 aatccaatcg tctggcgaca ttattattgt tgcgcgagct gggcatggac gccgtggacg
     3961 gcggtacggt gcgcgacgat ccggacgcgt tgcgtgcctg gctcaagtcc tccacggatt
     4021 acgacttcgt catcaccagc ggcggcgcct cggtgggcga tcatgatctg gtgaagcagg
     4081 tgttcgccga gatcggcgcg atcaagttct ggaaggcgaa gatcaaaccg ggcaagccga
     4141 tcgccttcgg gcgcgtggga atgcgcacgc atttcttcgc gctgcccggc aatccggtat
     4201 cgagtctcgt gactttcaaa ctgttcgtcg agccggcgct catcgcctgg catcacggtg
     4261 tttcacatat gccgcaactg gccgccaccg ccaccaacga tttccggcgt cacccgggcc
     4321 gcaccgagtt cgtgcgcgcg cggctcgcca ccgaagacgg caaacttctg gccaccgccc
     4381 tgcgcggtca gggttcgcac atgctagggc cgctgcgcga gaccaacggc ttcatccgcg
     4441 tcgaggccga cagcgccggt ttccagcagg gcgagacagt tgcagccatt cccttgaaca
     4501 cggatttttg atggcgatga atattgcaac gcatgatatt accggcgtca ttctcgccgg
     4561 cggccgcggc agccgcctcg gtggcgtgga caaaggcctc gtgcgactgc gcggccgccc
     4621 gctcattgaa tacgccatca atgccctgcg gccgcaagtg ggcagcctgc tcatcagcgc
     4681 caatcgaaat cgcgatgtct acgcatccta cggatatcac gtcatcgccg atgtcatggg
     4741 caactacgat ggaccgttgg cgggaatgtt gagcgccatg cgtcatgcgg acactcccta
     4801 tattgtcacg gcgccttgcg acgtgcccgc accgcccgtc gacctcgtcg aacgacttgc
     4861 cggcgctatg agcatgacac aacgcgaggc atgcatcgct tcctgtgaag gacaaacaca
     4921 gccggtattt gcgctcgtgc gttgcgccct ggccgatcgc ctgcaggaat atcttgcggc
     4981 gggcgaacgc ggggtggaga actggatgcg tcagcagcag gcggcgatcg tggattttcc
     5041 agaacacgat gctttcgtga acatcaatac ggaggaagat ctgcgtcgcc tggaagcggt
     5101 ggcgtgaccg gcacagcctc aggcgtctgt tgtcgtccgg cgttcgacag gtaattgcac
     5161 tgtcgccaga aatccgccca ggatttccga gcggccgaag cccatgcgcc cgccgtaatc
     5221 cgacgccgtc tgcatggcga tggcgagccc caggccctgg cccggcatgg attcgtccag
     5281 ccgtgtacca cgctgcgcaa gggaggccag gctttcatcc ggacaaccgg gcccatcgtc
     5341 gtgaaccctg atttctatca gcgccttttc cccctcaccc catccctctc ccgcgagggg
     5401 agagggggag tcggtcgtcg aggtgaaagt cacctcgacg tacacttcgg atcggcccca
     5461 tttgcaggcg ttgtccagca gattgcccag cagctccagc atgtcctcgc gatcggctgg
     5521 aaacgaaacc gcggctggca tgcgcatgct tatctcaagc ggtttttcgc ggtaaagggc
     5581 tttgagcgtt tcaatgagcg ccggcatttc ggtggcaaaa tcaaaacgcc ggccggggag
     5641 ggtggcgccg gagagccgcg cccgcttgag ttcgcgctcg atgagatact tcatggtctg
     5701 gagctgttcc gccatggctt ggcgcggcgc cgcggcatcg gtcttttcct ccgcgacctg
     5761 atgcgtcagc agcgtgagcg gtcccttcag ggcatgcgcg agattgccca gggcctggcg
     5821 cgaccgcgtc aggcgttgcg tcatcgccgt aagcaaacgg ttgagttctt ttaccagcgg
     5881 cgacagttcg gcgggaaccg cttcactcaa ccggctgacc tcgccctgtt ccagctgctt
     5941 cagttccgcg cgcgtccggt gcagcgggcg caaaccgcgg cgcaccagcc atgcctggag
     6001 tgccagcagc agcgccagcg cggcgagcga gaccagcgcg tagcgattgc ggaactctcg
     6061 catatcgttc tccagcggcg tgaggtcttc ggcgacggag ataacaaggc gtcggccccg
     6121 ccattcgaag ttccgtgtgt acatgatcag ggactgcgat tgcggccccg ggaggcgttg
     6181 cgtcaggatt ccgcccgtgg cgtcggagac ggcaggcagg tcctgatccc acagcgaacg
     6241 cgagcggatc gtctgatcgc cggcggcgat gcggtaatag ctgccggaga agggctgctg
     6301 gtaaaccttc tcgatacgat ccggtgccag ctgcatgcgc ccggccgcat cgaaggaaat
     6361 cgcggtgagg agattttcga tatcatgctc catgtggacc gtgaagcctg actcgctcag
     6421 gtggcgcaag gtcaccgaca ccaccagcca ttgcaggaag aacaccacca cgagactgac
     6481 ggcaagactg acaccaagat gtttctgaat ggagcgcatc aggagttggc gccgaagata
     6541 tatccctggc cgcgacgggt gtgaatcacg tcgggatcga gtttttcccg caaccggcgt
     6601 atgtagacct cgatcacgtt gctgtcgcgt tccgagtcga aatcgtatac gtgcccggtg
     6661 agttcggttt tggaaaggat tttccccgga tgcagcatga aatagcgcag caggcggaat
     6721 tccgtgccgg tcagttcgat ggtcttcccg tggtacgtaa ccgtctgccg ttcctcgtca
     6781 agcacgagat ccgccgcctg gatgccgcct ttcggttcgc cgtgtcgacg gcggatgatg
     6841 gcgttgaccc gtgccaccag ttcttcgaag tgaaacggtt tggcgagata gtcatccgcc
     6901 ccggccttga agccgtccac cttttcctgc caggtgtcgc gcgcggtcag aatgatgacg
     6961 ggcatcgtct tgcctcgcgt ccgccagtgc ctcagcacct cgagccccgg gcgccgtggc
     7021 agcccgagat cgagcaccac ggcgtcgtaa ggttcggtgt cgccgaggtg ttcggcgtct
     7081 tcgccgttgt cggtggtgtc gacggcgtag ccggcctgct ccagcgcctg ttcgaggctg
     7141 gcgcaaagtg ccgcgtcgtc ttccaccacc agcagtcgca ccttattttt ccttttcagc
     7201 cttcagcaat tcaccgtttt tggcgttgta ctccagttca tgcaccacgc ctttttcatc
     7261 cacgatcttc acttcgtaga catggcgcct ggattttctt tccagttccg tttccaccac
     7321 gcggccgggt tgtttctgtt ttgccttttc caggatctgt tccagcggca ggatatcgcc
     7381 ggcctcccgg agttttttgg cctcgtcctg gctttcactg gcgaaaccgg gcacggcgaa
     7441 cgtcagcaga accgcggcgg ttaatagagt cttgggcagt gttttcatgg taatactcct
     7501 ctgggttaat ggaaatatca gacgcgcttg cgtccggtga acatggcgcg caccaggttt
     7561 tcccggtgca ccaagctgcc gatgatcacg ccggcgatat gcagcgcgac cagcagcagt
     7621 gtcaggttgg cgaagaactc gtgcagctct tcccgcaatt cctcgtggcc ctcgttttct
     7681 tcccctccgt tcccgccttc gtcgtcgtcc gccacggcct tggcgatcac gctgggcatc
     7741 gccaccgcgg cgccggtcga gagagaggca aatggtccgg tgccttcctc cgcaccttgc
     7801 agggcaagcc ccgtgacgcc ggtggcaagc agcgacacca gcagcgcgat gaccatcact
     7861 ccgccaatgg ggttgtgccc caggtagcgc ccgggttttc cgatcagcat ttccctggcg
     7921 taacccagaa cggtggaagg gcggtagaca aaatcgctga aacgcgcgta acgtgtgccg
     7981 atgaatcccc agacgatgcg caccagcacc aggccgatga cagcataccc agccaattcg
     8041 tgcaggcgcg attcctcgtc cgcggtcagc cacgcgataa tgaatgtcgc caccagcgac
     8101 cagtgaaaca ggcgcaccag cggatcccag accttcacgg cagccggggt tgacggggtt
     8161 tcaggcgggt tcataacggt ctcctcaagc aaggtaatga gatatcgctg cgaacagtag
     8221 cagccgaagc tgaaatcaaa ctgaaaggcg cgggacgacg ggggactgtg ttacccttga
     8281 gacaacgata gggcggccgc actcaagtaa tacatactat tttcaggagc caccatgccg
     8341 gaactgtccg aaaagcagct gcaagacttc acgcaacgcc tgagaaatcg gcgcgaggaa
     8401 ctgcgcgata ttattcgcga ggagttggcg agcacgcagc gcgaggacta taccgagctg
     8461 gccggcatgg tgcacgacgc cggggatgag tcttttgcgg aactgttgcg tggcatcaat
     8521 ctcaccacgc gcgcccgtga actcgaggaa atccaggacg tggaggccgc gctcgagcgc
     8581 atcaagaacg gcatttacgg cagctgcgtc gattgtaacg acagcatcgc cctcgaacga
     8641 ctcgatgcct atccgacggc gaagcgttgc atcaactgcc aaagccggca cgaaaaccgt
     8701 aggggtgggc gcgacgccac cccaagcctg taaaagccgt ttcaaaacct gctgcgcgtc
     8761 cgtgatccgg ggtcgagcgg tgctcggaat cctcatgtac tggcgtgtac actccggttc
     8821 ctgcgctccg ttctcccccg gctgacggcc gctcgcgacg gttttgaaac gacttttgcg
     8881 ccaatcaccg ctgtttattt cccttcgact gtttttacga agcgtaccag cgcgtgccgg
     8941 acacgcatca gagggtggcg gtgagcgggc gtccggtcag caggacttcc agcaagtcgc
     9001 gcaccttgcg tatctccgcg ccaaagggca gtgattccga cccgcggaag aacaatccct
     9061 gctgaatatt gccgcgctgc gccgcggcga gctgcgtctc gatgcagaac tggccggcgt
     9121 ggggattgcc gtccttgaag ccgcaatggg cgaggcattc gagccggctc gggcattggg
     9181 agcattcggg actcgctttg gcccgcaatt tttcttcctt cgccagatag tttttcagcc
     9241 acggggtcag caccgcgcgg gccggcagac cggcggcgct catgaacgtg acgatctctt
     9301 ccggtttcgc ctcggccagc accttcttga agttggggtg cgcgtcgcat tcctcggtga
     9361 ccgcgaacgg tgtgccgatc tgcgcgcccg cggcgcccat gtcgaacagc tccttgagtt
     9421 tctgaaacga gttgatgccg cccgcgggta ttaatggaat actgccaagc gcaattccca
     9481 actcctgaaa tacgcgccgg atttcctcca gtacccttcc aaagtcgaat ttcggatcgt
     9541 tcacttcttc catgcgcgtg gcgccaagat ggccgccggc atagcgtgga tgctcgatga
     9601 cgatggcgtc cggcagccgt cccttgcgca tccatttctt cagcaccgcg cgcacgccgc
     9661 gctcctccga aaggatcgga atcaacgcga catttttgaa tttttccgtg agctcgggca
     9721 gatcgaacgg cagccccgcg cctgaaatga tggcattggc gccgctcttg cacgcctgca
     9781 ccaccagctc ggcataattc gacagcgcac gcatcacgtt caccgcgacg aagccgtccg
     9841 gtgcgatttc gtgcgcggcg cgaatctcgc gatcaagtgc ctcgatgttc gcggcgctgt
     9901 gcaccttcgg atcgtgacat ttgcgcaggc gctgcatgat atccgggtac aggcgtctca
     9961 gatccacgct ggcgatggtg ccgacggcac cttccttcgc aacggcaccg gcaaggcggt
    10021 gtccggaaat gcccacgccc atgccgccct ggatgacggg cagcaattca cgaccgcgga
    10081 gcgtgagcag gggcaggggg gtacggatca tgacgagatg ggcctcaatg gaaaaacaaa
    10141 ctagcctatt tccgtgaaaa tagggttgac gcaggtcaag ctattgcacg agagcggaag
    10201 cagaccgtca ggcaacgaaa ggcaatacct gacgggtgag tgcgaccaga acgatataaa
    10261 ggaatatggc gatcgcgccg agccaggcgg tgatgcggat ttttttcgtc cggccgtatt
    10321 tgaggccgac ggcaccgacc acgatgtagg cgaccagcgc cagcaccttg gccgtgagcc
    10381 agccatgcac gaaagggtat tgcctgattt gccacgccag cagaatggct gaaaccagca
    10441 gggtcgtatc aataatatgc ggcagtaccc gtacccattt ttcatgcagc cggggcgatt
    10501 cgcggatcat ccagatgcca cggatgaaaa aaccggtgcc ggaaaggacc acgcaggtaa
    10561 cgtgaatata tttgagccac cacatcggat gaaggtctgt tgttatccgg gtttcccgtc
    10621 cacgcgccga tgcacgtaca tcggcgcgta aagccaggcc cacaacagga agga
//
LOCUS       DBNGZE010000115         2199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1127307, whole genome shotgun sequence.
ACCESSION   DBNGZE010000115 DBNGZE010000000
VERSION     DBNGZE010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1127307"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16FV_02970"
     CDS             <1..377
                     /locus_tag="AC16FV_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2849059.1"
                     /translation="RPDQYRTIFTKNPPNALLAVYNGEADAAGVSMPVLQRPEIRQRI
                     DVNQLRILDESREIPHLPVAVRGDMDAQLSRRIQRILTGLTRHSEGHSILEQMGVQRF
                     ATTDDHEYDIVRRLMKEEDGAY"
     gene            367..>2199
                     /locus_tag="AC16FV_02975"
     CDS             367..>2199
                     /locus_tag="AC16FV_02975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2849060.1"
                     /translation="MRINRNQPWRHGLRARFLPVLGIFVAVSMAVLGTSLFFNQRQIT
                     LDRFEQDTRNLRQVLQDKGNAYSTFLARIAPQGILSHDYLLLEGYVEELSSDSDVVYA
                     VILNRANQPITHFLKKNDAPHDPANAVNVMPEHFMQSLAKARANKELLIIKRKILYDD
                     INLGNVEVGLSQAKIAHSLDALKIDLDRELRRIATLTGGGILIALVILILLSGRAFNR
                     LVARPIQALGAEMARVQSGDLGARTTINREDEIGWLARSFNTMAADLQSYVEKIEQQR
                     RVEKETRDYLANILDNSADMIATTGLDGSIVEFNAAAERILGYRRDEVVGKDSSVFYC
                     DRKMRESLYASVKDSKPVQNTETRLRCKNGRIIDAELTVSPLHDNAGELIGAVCIGRD
                     VTQAKTMRRELIQAEKMATVGQVSAWIAHQIRNSLGRILMSASALRPDRDSADSRHQA
                     HHDLTSSIAEMDKIVSDLLDYSRTLSLHPTRISLNAALEGLLGSLAVDGVNGHHHIER
                     VFDPDLPPIQIDVFKMEQALGNVLKNAMQAMPDKGTLRVETRRGPGEQQVTVLVRDSG
                     PGIPHENLQKVFRPFFTTKPGGTGLGLAIASRVVEAHGGSVMVDS"
BASE COUNT          505 a          643 c          634 g          417 t
ORIGIN      
        1 tcaggccgga tcaataccgc accatcttca ccaaaaaccc gcctaatgcg ctgttggccg
       61 tatacaacgg cgaggcggac gccgcgggcg tgagcatgcc ggtgctgcag cgaccggaga
      121 tccgccagcg catcgacgtg aatcagttgc ggatactgga cgagagtcgc gagattccgc
      181 atttaccagt cgccgtgcgc ggcgacatgg acgcgcaatt gtcgcgacgt attcagcgga
      241 tactcaccgg cctgacacgg cattccgagg ggcattccat actcgaacaa atgggcgtcc
      301 agcgcttcgc gacaacggat gaccatgaat acgatatcgt cagaagattg atgaaggaag
      361 aagacggtgc gtattaaccg taaccaaccc tggcgacatg gtctgcgggc gcgctttctg
      421 ccagtgctcg gcatattcgt ggccgtctcc atggcggtgc tgggtaccag cctgtttttc
      481 aaccagcgcc agatcacgct cgaccgcttc gaacaggaca cgcgcaacct gaggcaggtg
      541 ctgcaggaca agggcaatgc ctattccacc ttcctcgcgc gcatcgcgcc gcaaggaatt
      601 ctttcacacg attacctgtt actcgaaggc tatgtcgagg agctgtcctc cgattctgat
      661 gttgtgtacg ccgtcatact caatcgggca aatcagccca tcacgcactt cctgaaaaag
      721 aacgatgcac cgcatgatcc ggcaaatgcc gtgaatgtga tgcccgaaca tttcatgcaa
      781 tcgctggcaa aagcccgcgc aaacaaggaa ctgctgatta tcaaacgcaa aatactctat
      841 gacgatatca acctgggcaa cgtggaagtc ggactttccc aagccaagat tgcgcacagc
      901 ctggatgcgc tcaagatcga tctggaccgg gaattgcgcc gcattgccac attgaccggc
      961 ggcggaatcc tgatagcgct ggtaatcctg attcttttga gcgggcgcgc ttttaaccgg
     1021 ctggtggcgc ggccgattca ggcattgggc gccgaaatgg cgcgggtcca atcgggagat
     1081 cttggggcgc gcaccaccat caaccgggag gacgaaatcg gctggctcgc ccgcagcttc
     1141 aacacgatgg cggcggattt gcagagctac gttgagaaga tcgaacaaca gcgtcgcgtt
     1201 gaaaaagaga cgcgcgacta cctcgccaac atcctcgata attctgcgga catgatcgcc
     1261 actaccggcc tggacggttc catcgtcgaa ttcaatgccg ccgccgaacg catactcggt
     1321 taccggcgtg acgaagtggt ggggaaagac tcgtccgtct tctattgcga tcggaaaatg
     1381 cgcgaatcgc tgtatgcctc ggtgaaggac agcaagccgg ttcagaacac cgaaacccgg
     1441 ctgcggtgca aaaacggcag gattatcgac gcggaactga cagtctcgcc attgcacgat
     1501 aacgctggag aactcatcgg cgccgtttgt atcgggcgtg acgtcactca ggccaagacc
     1561 atgcgtcgcg aactgattca ggcggaaaaa atggcaaccg tcggacaggt ctcagcgtgg
     1621 atcgcgcacc agattcgaaa ctcgctcggg agaattctca tgagcgcttc ggcgctgcgc
     1681 ccggatcgcg actccgcgga ttcacgacac caggctcacc acgacctgac atcctccatc
     1741 gccgagatgg acaagattgt gtccgacttg ctcgactaca gccgcacgct ctcgctgcac
     1801 cccacccgca tcagcctcaa tgccgcgctt gaaggtctgc tgggttcgct cgccgtcgac
     1861 ggcgtgaacg gtcaccatca catcgagcgc gtattcgacc cggatttgcc gccgatacag
     1921 atagatgtgt tcaaaatgga acaggcgctg ggcaacgtgc tgaagaatgc catgcaggcc
     1981 atgccggaca aggggacgct gcgggtggaa acacgccgcg gacccggtga gcaacaagtg
     2041 accgtgcttg tccgggacag tgggcccggc atcccgcatg aaaatctgca aaaagtgttt
     2101 cggccttttt tcacgaccaa accgggcggc accggactcg ggctggctat tgcttctcgt
     2161 gttgtcgagg cgcatggagg cagcgtgatg gttgacagc
//
LOCUS       DBNGZE010000116         3424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1891327, whole genome shotgun sequence.
ACCESSION   DBNGZE010000116 DBNGZE010000000
VERSION     DBNGZE010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1891327"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="AC16FV_02980"
     CDS             complement(<1..769)
                     /locus_tag="AC16FV_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010373997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR2849061.1"
                     /translation="MAAQDTYNYRVVRQFAVMTVIWGIVGMLVGVFIAAQMAWPVLNF
                     DIPWLTFSRLRPLHTNAVIFAFGGSALFATSYYVVQRTCHVRLFSDGLAAFTFWGWQL
                     IIVLAAVSLPLGITSSKEYAELEWPIDILITLVWVAYAVVFFGTLIKRKVSHIYVANW
                     FYGAFILTIAVLHIFNNLEVPVSWTKSYSVYAGVQDAMVQWWYGHNAVGFFLTAGFLG
                     MMYYFIPKQAERPVYSYRLSVVHFWALAFTYIWAGPHH"
     gene            complement(907..1506)
                     /locus_tag="AC16FV_02985"
     CDS             complement(907..1506)
                     /locus_tag="AC16FV_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HIR2849062.1"
                     /translation="MLNHVWGLLFHPAEEWKSIRKENCTIGKCYCSHVLLLAAIPPLA
                     GYIGTTQVGWQVVSREVHTLTPQSALWIAILSYITILVAVFLIGKTIHWMGQTYGAKQ
                     TLPQCIALAAYTATPLFLSGIMLLYPMLWLNLLIGLPVLGYTVYLLYTGVPIIMGIPE
                     ERGFLFSTAVLAVGLVTLVAVLAATVILWDIGAGPVFAG"
     gene            complement(1667..1789)
                     /locus_tag="AC16FV_02990"
     CDS             complement(1667..1789)
                     /locus_tag="AC16FV_02990"
                     /inference="COORDINATES: protein motif:HMM:NF022790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HIR2849063.1"
                     /translation="MPILDELFGTWIGILSLLTIAFILGMGVFIFLYVRRQMNK"
     gene            complement(1811..2449)
                     /locus_tag="AC16FV_02995"
     CDS             complement(1811..2449)
                     /locus_tag="AC16FV_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW/AlkL family protein"
                     /protein_id="HIR2849064.1"
                     /translation="MNTALRTRHIAGALLALGFAATAVPAHAVQKGDWLIRGGVGHVA
                     PTGESGTVPVVGGKVEADSSTNLAINFTYMLNDNLGVELLGALPFKHDITHDSLGKVG
                     ETKELPPTLILLYNFAPKSGVRPYAGIGLNYTTFFSEKTTGALAGTSLKLDDSFGLVV
                     EAGVDVDINKDWFFNASLWRMDIDTEADSSLLGKFDVAIDPWAFFVGVGKRF"
     gene            complement(2667..>3424)
                     /locus_tag="AC16FV_03000"
     CDS             complement(2667..>3424)
                     /locus_tag="AC16FV_03000"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR2849065.1"
                     /translation="NSAPGRLAAFLLGLSERFRERGFAAQEFRLGMSRVDIGNYLGLA
                     DETVSRLFTRFQEEGLLAVERRHIRLLDVPRLQAVARGQPERASSKVYIGGGMYSKPS
                     MEPDAPEGDDEAGFPAWDPGYRIGVDVIDQQHQKLFDISHRFYDAWRQHARRARLRHI
                     FDELLEYTAYHFAEEERLMQQIGYPDLPQHRANHEELVELVNYHRKQLAEDAPDAERH
                     ALEFVRTWLRAHVLDADKDIGVYLAGRRAESGG"
BASE COUNT          715 a         1024 c         1066 g          619 t
ORIGIN      
        1 ggtggtgcgg gccggcccag atgtaggtga acgccagcgc ccagaaatgc accaccgaca
       61 ggcgatacga atacaccggc cgttccgcct gtttcgggat gaagtaatac atcatgccga
      121 ggaagccggc ggtgaggaaa aagccgaccg cgttgtggcc gtaccaccac tgcaccatgg
      181 cgtcctgcac gccggcatag accgagtagg acttggtcca cgataccggc acttccagat
      241 tgttgaaaat gtgcagcacc gcgatggtga gaatgaacgc gccgtagaac cagttggcga
      301 cgtagatgtg gctaaccttg cgcttgatca gcgtgccgaa gaacacgacg gcataggcga
      361 cccacaccag cgtgatcagg atgtcgatcg gccattccag ctcggcatat tccttggagc
      421 tggtgatgcc gagcggcagc gaaacggccg ccagcacgat gatgagctgc cagccccaga
      481 aggtgaacgc cgccaggccg tcgctgaaca ggcgcacgtg gcaggtgcgc tgcacgacgt
      541 aataggaggt ggcgaacagc gccgaaccgc cgaaggcgaa gatcaccgcg ttggtgtgca
      601 gcgggcgcag acgcgagaag gtgagccagg ggatgtcgaa gttcagcacc ggccatgcca
      661 tctgcgcggc gataaacacc ccgaccagca ttccgacgat accccaaatg acggtcatca
      721 cggcaaactg ccgtacgacc ctgtaattgt acgtgtcctg ggcggccata attctcccgc
      781 tattcagtgt gccaatttaa aatttaaaat gattgttgaa aagttgttgt tgcatcaccg
      841 cactgacccg gccagcggtg aaactactat ggatgcgggc ggcgtctcgt tgacgcagat
      901 caaactctag ccggcgaaga ccgggccggc gccgatgtcc catagaatga cggtcgccgc
      961 cagcaccgcc accagcgtca ccaggcccac ggccagcacc gcggtggaga aaagaaagcc
     1021 gcgctcttcg ggaatgccca tgatgatcgg cacgccggtg tacagcaggt acacggtata
     1081 cccgagcacc ggcagaccga tcagcaggtt cagccacagc atcggataga gcagcatgat
     1141 gccggagaga aacagcggcg tagccgtgta cgcggcgagc gcgatgcact gcgggagtgt
     1201 ttgtttggcg ccgtaggtct ggcccatcca gtggatggtt tttccgatca ggaacaccgc
     1261 cacgaggatc gtgatgtagg acaggatcgc gatccagagc gcgctctgcg gcgtcagcgt
     1321 gtgcacctcg cgcgaaacta cctgccagcc gacctgggtg gtgccgatgt aaccggccag
     1381 gggaggaatc gcggcgagca acaacacgtg ggaacaataa cacttgccga tggtgcagtt
     1441 ttccttgcgg atggatttcc attcctccgc cggatgaaac aacaaacccc acacgtgatt
     1501 caggatcatg cttttctccc acctgctgta ttgattgcgc ggccagtttc ctgcaaaggc
     1561 caaggggcgt caatacgctt ggaatcgcca agcgcgacat aacaaagctg gatggtcgga
     1621 tatccgcggt acgccgggtg acacgaaaca tggcaaatgc tccttgttac ttgttcatct
     1681 ggcggcgcac ataaagaaat atgaatacgc ccatcccgag aatgaaggca atggtcaaca
     1741 gggacaggat accgatccac gtgccgaaca attcatccag gatgggcatg gctttgctcc
     1801 ggggcgcgct tcagaagcgc ttgccgacac cgacgaagaa ggcccacgga tcgatggcca
     1861 cgtcgaactt gccgagcaat gacgaatctg cctcggtgtc gatgtccatg cgccagagcg
     1921 aggcgttgaa gaaccagtcc ttgttgatgt cgacgtcgac accggcctcc accaccaggc
     1981 cgaaggaatc gtccagcttg agcgacgtgc cggccagggc ccccgtggtt ttttcgctga
     2041 agaacgtggt gtagttcagg ccgatgcccg cgtagggacg cacgccggac ttgggggcga
     2101 agttgtagag caggatcagc gtcggcggca gctccttggt ctcgcccacc ttgccaaggc
     2161 tgtcgtgcgt gatgtcgtgc ttgaacggca acgcgcccag cagctcgacg cccaggttat
     2221 cgttgagcat gtaggtgaaa ttgatcgcga ggttcgtgct gctgtccgcc tcgaccttgc
     2281 cgccgacaac cggcacggtg ccgctttcgc ccgtgggcgc gacatgtccg acaccacccc
     2341 ggatcagcca gtcgcccttt tgcaccgcgt gcgcgggtac ggcggtggcg gcaaaaccga
     2401 gcgccagcag cgctccggcg atatggcgtg tacgtaatgc tgtattcatg atacccccca
     2461 acaagtgtga gtgaccgagt cattcgcgcg gaattcgact ggtcgctatt cgcatcacag
     2521 cgtcattccg ttcgccgcca ttctctatgt tgcatggcgc cggacactga cctgggtcag
     2581 gaatgcaatc gttttccatg cagcgaatcg cgtgggacga cacaggggac tacggttgag
     2641 tgggggatga ggagagaagc gggttttcag ccgccgctct cggcgcggcg gccggccaga
     2701 taaacaccga tgtccttgtc ggcgtccagc acgtgcgcgc gcagccaggt cctgacgaat
     2761 tcgagcgcgt gccgttcggc gtccggcgcg tcctccgcga gttgcttgcg gtggtaattc
     2821 accagttcca ccagctcctc gtggttcgcg cgatgttgcg gcaagtccgg atagccgatc
     2881 tgctgcatca gccgttcctc ttcggcgaaa tggtaggcgg tgtactccag cagttcgtcg
     2941 aagatatggc gcaatcgggc gcggcgggca tgttgccgcc aggcatcgta aaaccggtgg
     3001 ctgatgtcga acagcttctg gtgctgctgg tcaatgacat cgacgccgat gcggtaaccc
     3061 gggtcccagg ccgggaaccc cgcctcgtca tcgccttccg gcgcatccgg ttccatggac
     3121 ggcttcgaat acatgccgcc gccgatataa accttcgacg aggcgcgctc gggttggccg
     3181 cgcgccactg cctgcaggcg cggcacgtcg agcagccgga tatgccggcg ctcgaccgcg
     3241 agcaggccct cttcctggaa acgcgtgaac agccggctca ccgtttcgtc ggcgaggccg
     3301 aggtagttgc cgatgtcgac gcgcgacatg cccaaacgaa actcctgcgc cgcgaagccg
     3361 cgctcgcgga aacgttcgga caggcccagc agaaacgccg ccagtcttcc gggggcgctg
     3421 ttct
//
LOCUS       DBNGZE010000117         1963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1614510, whole genome shotgun sequence.
ACCESSION   DBNGZE010000117 DBNGZE010000000
VERSION     DBNGZE010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1614510"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1644)
                     /locus_tag="AC16FV_03005"
     CDS             complement(160..1644)
                     /locus_tag="AC16FV_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849066.1"
                     /translation="MDSLQALLIWPDNLLLSLLVLFFAAVPILYGARQAMHSLLQALF
                     RGISNPLRLMSHWLFKTADTLRERNKIVLLSRGREEVAHGIAKEFDRVTALVERDLHG
                     YPALQRKLVDEITRIEEEFQKCGEVPPPPPEWTKAVAAIAKIKPSSDGLVERILEDIS
                     NSIDEIYEKIVAEYRESYKQRHNILKGFMPFWRSVDQTLKRVDKNLTGMQQSAARIDE
                     QVEKYKQIHAKSEQAEHTLTSSSTILFVISLLAIMIAAGGAFVNFWLIERPMAAMVGG
                     SEYIVGNIQTSYLAAMVIIFFETLMGLFLMESLGYTHLFPLNNINVRMRRWLVWISFS
                     ILLILAGVEVALAVMRDMIISADIALKQELAGSNAVVTAVANSWALDIPKAAQMILGF
                     TLPFALAFVAIPLEYLIHSGRTVFGALLVLGIRGLGFVTRFLSSMSRQIGKGVEHLYD
                     AIIFLPLIIERAVLNRGPRQVSASGGSGKVTSFSKHGTPGEHVL"
BASE COUNT          437 a          554 c          586 g          386 t
ORIGIN      
        1 gcagatagct cacgacgcgc tgggccgtgt cgagttcctt tgaatacgtt cccgaagtat
       61 ccaccagcat gtacaccgct tgcgcatgac tgcgcggatc ggcgcaagag gtcgcgagca
      121 gacccaaggc cagaactaga ataaaccaca ggcggcgcat cacagcacat gctccccggg
      181 ggtgccgtgc ttggaaaaag aggttacctt ccccgatccg cccgaggcac ttacttgtcg
      241 cggtccgcgg ttcagcaccg cgcgctcgat gatgagtggg agaaaaataa tggcgtcgta
      301 aagatgttcc acacccttgc cgatctggcg cgacatgctc gacaggaagc gcgtgacgaa
      361 gcccaggccg cggataccca gcaccagcaa cgcgccgaat accgtgcgcc cggaatggat
      421 caggtattcc agcgggatgg ccacgaaagc cagcgcgaag ggcagggtga agccgagaat
      481 catctgggcc gccttgggaa tatccagcgc ccaggaattg gccaccgccg tgaccacggc
      541 gttgctgccg gccagttcct gcttcagggc gatgtcagca gaaataatca tgtcgcgcat
      601 cactgccagc gcgacttcga cgccggcgag gatcagcagg atgctgaagg agatccacac
      661 cagccagcgg cgcatgcgga cgttgatgtt gttcagtggg aacagatggg tgtaacccag
      721 ggattccatc aggaacaaac ccatcagcgt ttcaaaaaag atgatcacca tcgccgccag
      781 ataggaggtc tggatgttgc cgacgatata ttcggaaccg ccgaccatgg ccgccatcgg
      841 gcgttctatg agccagaagt tcacgaaggc gccgcccgcg gcgatcatga tcgccagcag
      901 cgaaatcacg aacaggatgg tcgatgagga agtcagggtg tgctcggcct gttcgctctt
      961 ggcatggatc tgtttatact tttccacctg ctcatcgatt ctggcggcgc tttgctgcat
     1021 gccggtgagg ttcttgtcca cgcgcttcag ggtctgatcg accgaacgcc agaagggcat
     1081 gaagcccttg aggatgttgt ggcgctgctt gtacgactcg cggtactcgg cgacgatttt
     1141 ctcgtagatc tcgtcgatgg aattgctgat gtcttccagt atccgttcca ccagcccatc
     1201 gctggagggt ttgatcttgg cgatggcggc cacggccttg gtccattccg gcggaggcgg
     1261 cggcacctcg ccgcatttct ggaattcctc ttcaatacgg gtgatctcgt ccacgagttt
     1321 gcgttgcagc gccgggtagc cgtggaggtc acgctccacc agtgcggtga cacgatcgaa
     1381 ttccttggcg atgccatgcg cgacttcctc gcgaccgcgg ctcagcagca cgatcttgtt
     1441 gcgctcgcgc agcgtgtcgg cggtcttgaa caaccagtgc gacatcagac gcagcgggtt
     1501 gctgatgccg cggaacaggg cttgcagcag gctgtgcatg gcctggcgcg caccgtagag
     1561 aatgggtaca gcggcgaaaa acaagaccaa cagggacaac aagagattat ccggccagat
     1621 gagtaaggct tgcaggctat ccatagtttt ctctcccaac gtgaaacgcg gaagcgcccc
     1681 ctgactggaa tcgttcccgc gaatgtatga ggatattatt agcgttattc aggacctgag
     1741 ctgccttggt ccgaccaatg cgccaaacat ggggattact ggtctgtcat caggaaaaaa
     1801 caacgagaaa agcccggttt ttccggtata cgcgagcaag gacaaaggac taaactgcac
     1861 tcatgcgatt ttcgatctat cgcttcaatc ccgaagccga caaaaaaccg cgcatgcagg
     1921 cctatgagct gcccgatgac agcggggcgg tgatgctgtt gga
//
LOCUS       DBNGZE010000118         3452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_225696, whole genome shotgun sequence.
ACCESSION   DBNGZE010000118 DBNGZE010000000
VERSION     DBNGZE010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_225696"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="dmeF"
                     /locus_tag="AC16FV_03010"
     CDS             <1..944
                     /gene="dmeF"
                     /locus_tag="AC16FV_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HIR2849067.1"
                     /translation="TAHDLSRWTHNHVFDTGNLAAERGTRAVMWITAAMMVVEIAAGW
                     WYNSMALLADGWHMSSHAVAIGLSAFAYAAARRHAGDARFAFGPWKIEVLGGYTSALF
                     LLGVVVMMVIGSVERLLSPQPIHYTEAISVAAIGLVVNVVCAYILGSAHHGHDAHEHH
                     DHTGHHHHDLNLKSAYVHVLADAATSVLAIIALAGGWLYGWSWLDPVMGIVGAGLVAV
                     WAKKLLTETGKVLLDREMDHPVVKEIRKAVESGAGRGKVDITDLHVWRVGKQSYACAI
                     SLVTHDAGLTPGRAREWLSIHEEIVHATIEIHVCTTC"
     gene            complement(838..1242)
                     /locus_tag="AC16FV_03015"
     CDS             complement(838..1242)
                     /locus_tag="AC16FV_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849068.1"
                     /translation="MKQITRTFFTGLAVTLPVVATLYLLYWAALAIERALDGLLRFVL
                     PKVAYFPGLGVALGCGELESFARPARGGGGGGNDRWHHALKPATSNVLSRRADQALNK
                     SCRRESRWWHARFPRVSRATHVHGRVSDRHRA"
     gene            complement(1368..1880)
                     /locus_tag="AC16FV_03020"
     CDS             complement(1368..1880)
                     /locus_tag="AC16FV_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849069.1"
                     /translation="MILGMSVSTFTLVHVVISLIGIFAGLIVLFGMFRAKRLNNWTAL
                     FLVTTVLTSVTGFFFPRDQLLPSHVVGAVSLVVLAVAILAFYVYRLARSWRWIYVAGA
                     VAALYFNVFVGVVQAFQKLSLLQPLAPTQSEPPFLVAQAVVLAIFVVLGIVAVRSFHP
                     ETRAPMFGSV"
     gene            complement(1986..2495)
                     /locus_tag="AC16FV_03025"
     CDS             complement(1986..2495)
                     /locus_tag="AC16FV_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR2849070.1"
                     /translation="MNSTDHILGPASATVTVIEYADFECPYCRAAYPAVKMLLMHFQD
                     RLRFVFRHFPMHPHAERAAEAAETAAAQGKFWQMHDLLFENQQRLGDEYLRQYARQVK
                     LDLQRYDQDMDSRIHLQRVREQMNSGKQSGVRGHPTFFVNGVIEDVSFGVENLHKAIE
                     AILQPASMS"
     gene            complement(2593..>3452)
                     /locus_tag="AC16FV_03030"
     CDS             complement(2593..>3452)
                     /locus_tag="AC16FV_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HIR2849071.1"
                     /translation="VRLARAPMLNHWWQVPLYVTARGLTTSPIPYGDISFEIGFDFLD
                     HQLRIQTSDGAVRTLALAPRSVADFYREFMAALRALGLEIKIWTMPVEVSDPIPFDKD
                     QQHHSYVPEQAQRFWRVLQQADRVLQQFRCGFLGKSSPVHFFWGSFDLAVTRFSGRTA
                     PPHPGGIPHIANWVTREAYSHEVSSCGFWPGGGPVPEPVFYAYAYPEPEGFRNYPVRP
                     QVAYYSNDLREFLLPYEAVRRAANPDTMLLDFAQSTYEAAADPAGWDRHALEYAASEG
                     SKQSIEKQG"
BASE COUNT          708 a         1063 c         1044 g          637 t
ORIGIN      
        1 tgactgccca cgatctttct cgctggaccc acaaccacgt gttcgacacc ggcaacctcg
       61 cagcggagcg cggcacgcgt gcggtgatgt ggatcaccgc cgccatgatg gtcgtcgaga
      121 tcgccgccgg ctggtggtac aactcaatgg cactgctggc ggacggctgg cacatgagtt
      181 cgcacgcggt cgccatcggc ctgagcgcgt ttgcctacgc ggccgcccgc cgccatgccg
      241 gcgacgcgcg ctttgccttc ggcccctgga aaatcgaggt gctcggcggt tacaccagcg
      301 cacttttcct gctcggtgtc gtggtcatga tggtcatcgg ttcggtggaa cggctgctgt
      361 cgccccaacc gattcactac accgaggcca taagcgttgc agccatcggc ctcgtggtca
      421 acgtcgtctg cgcctacatc ctcggcagtg cccaccatgg ccacgatgca cacgagcatc
      481 atgatcatac cgggcatcac caccacgatc tgaatctcaa atctgcttac gtgcatgtcc
      541 tcgccgacgc cgccacgtca gtgttggcga tcatcgcgct ggccggcggc tggctctatg
      601 gctggtcatg gctcgacccg gtcatgggca tcgtcggcgc cgggctggtg gcggtgtggg
      661 cgaagaaact gctcacggaa accggcaagg tgctgctgga ccgcgaaatg gatcatccag
      721 tcgtgaaaga aattcgcaag gcggtcgaat ccggcgccgg tcgcggcaag gtggatatca
      781 cggacctgca tgtctggcgc gtcggcaaac aatcgtatgc ctgcgccatc agcctcgtca
      841 cgcacgatgc cggtctgaca cccggccgtg cacgtgagtg gctctcgata cacgaggaaa
      901 tcgtgcatgc caccatcgag attcacgtct gcacgacttg ttgagcgcct gatccgcgcg
      961 ccgcgaaaga acgtttgaag tcgccggttt cagggcgtgg tgccaccggt cgtttccacc
     1021 accacccccg ccacgcgcgg gacgcgcgaa agattccaat tcaccacagc ccagcgctac
     1081 cccgaggccg gggaaatagg ccaccttcgg caacacgaag cgcaataagc catccagcgc
     1141 gcgctcgatc gcgagcgccg cccaatacaa cagatagaga gtcgccacca ccggcagcgt
     1201 cacggccaga ccggtgaaaa aggtgcgagt gatttgcttc atggaatgtc ctccgctgac
     1261 ggtacccgcg ggcacttgaa cagagcgcgc cggccgcgcc caagcttgcc gcctggcacg
     1321 cgcttatatt aaaggggctg gggtcgatta ttcgttgttg tgcgggttca aaccgagccg
     1381 aacatgggcg ccctggtctc ggggtgaaac gatcttaccg ccacgatgcc gagcacgacg
     1441 aagatcgcca gcacgaccgc ctgcgcgacg aggaacggcg gctcggattg cgtcggcgcc
     1501 agcggctgca gcaacgacag cttctggaag gcctgcacga ccccgacgaa gacattgaaa
     1561 tagagtgccg ccacggcgcc ggcgacgtaa atccagcgcc aggaccgcgc gaggcgatac
     1621 acatagaacg cgaggatcgc gaccgccagc accaccagcg aaacggcgcc gactacatgg
     1681 gacggcaata gttgatcgcg gggaaagaag aacccggtca cgctcgtcaa gaccgtggtg
     1741 acgagaaaca gcgccgtcca gttattgagt cttttcgcgc ggaacatgcc gaacaggacg
     1801 atgagcccgg cgaaaatccc gatcaggctt atgacaacgt gcaccagcgt aaacgtggat
     1861 accgacatac ccaagatcat gacatgcctc ctttcgaatc gaagactttt ttcaaggaca
     1921 ccacccggat ctttccgatg gcgtttaacg cctgaacaaa cgcattcaac tcggtcttta
     1981 ctgaactaac tcatgctcgc cggctgaagt atggcctcta tcgctttatg aagattttct
     2041 actccgaagg acacgtcctc gatgacgcca ttcacgaaga aagtcgggtg tccccgaact
     2101 ccgctctgct tcccgctgtt catctgttcg cgcacacgtt gcaggtgaat gcgactgtcc
     2161 atgtcctggt cgtagcgttg caaatccaac ttgacctgac gggcgtactg cctcaggtat
     2221 tcgtcgccca aacgctgctg attctcgaag agcaggtcgt gcatctgcca aaacttgcct
     2281 tgggcagccg ccgtctctgc cgcttccgcc gcgcgttcgg cgtgcggatg catggggaag
     2341 tgccgaaaca cgaaacgcaa gcggtcctgg aaatgcatga gcagcatctt cacggcggga
     2401 taggcggcgc ggcagtaagg acattcgaag tcagcgtatt cgataacggt aaccgtggca
     2461 gacgccggcc ccagaatgtg gtcagtggag ttcaacggaa gcagaaacgc tgtttccttt
     2521 tgttctggcg gcagtcgacc tccattttcg atgtcacgaa gatcaagggc tcagaataat
     2581 tgaaatatga agctaacctt gcttttcaat actctgtttg cttccctccg acgcggcgta
     2641 ttccaaggcg tgacgatccc agcctgctgg atcagcggcc gcttcataag tgctctgcgc
     2701 gaagtcgagg agcatggtgt ccgggttcgc ggcgcggcga acggcttcgt acggcagcag
     2761 gaactcgcgc aggtcgttgc tgtagtacgc gacctgggga cgcacggggt aattcctgaa
     2821 tccttccggc tccgggtagg cataggcata gaagaccggt tccggcaccg gaccgccgcc
     2881 gggccagaag ccgcaactgc tcacttcatg cgagtaggcc tcgcgtgtta cccagttcgc
     2941 gatatgcggg atgccgcccg ggtgcggggg cgccgtccgg cccgagaagc gcgtgaccgc
     3001 gaggtcgaaa ctgccccaga aaaaatggac cgggctggat ttgccgagaa acccgcagcg
     3061 gaactgctgc aacacgcggt cggcctgctg cagcacgcgc cagaagcgtt gcgcctgttc
     3121 cgggacatag ctgtgatgtt gctggtcttt gtcgaacggg atcgggtccg acacctctac
     3181 cggcatggtc cagattttta tttccaggcc gagcgcgcgc agcgccgcca tgaactcgcg
     3241 atagaagtcg gcgaccgagc gcggtgccag cgcgagcgtg cgcacggcgc cgtcgctggt
     3301 ttgtatccgg agttgatggt cgagaaagtc gaaaccgatc tcgaagctga tgtcaccgta
     3361 cgggatcggc gaggtggtaa gcccgcgcgc ggtcacatac agcgggacct gccaccagtg
     3421 attgagcatc ggcgcccggg ccagccgcac ct
//
LOCUS       DBNGZE010000119         3959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1751126, whole genome shotgun sequence.
ACCESSION   DBNGZE010000119 DBNGZE010000000
VERSION     DBNGZE010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1751126"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16FV_03035"
     CDS             <1..609
                     /locus_tag="AC16FV_03035"
                     /inference="COORDINATES: protein motif:HMM:NF023701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HIR2849072.1"
                     /translation="PYATEDIDIARREALAFEKPPEQGLLEMLKDSGIPFIEVPSLNR
                     KKPPTSFKQRGRSRFHVDLLVPSANEDFSVVPVPELKAHATGLPYLSYLLAESQTAML
                     MAREGCCPVRVPLPERFAVHKLIVSRLLTGREAKSDKDVFQACVLCAALAESHPDAIE
                     SAVAQIPLRAKKYLPAAVESARRFLEPAHGRAWEELSGAMTS"
     gene            complement(665..1093)
                     /locus_tag="AC16FV_03040"
     CDS             complement(665..1093)
                     /locus_tag="AC16FV_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR2849073.1"
                     /translation="MLLLLLLLIPIAEIAVFIQVGSRIGVGMTLFLVLASAVFGVWLV
                     RTQGFAAATRVHAMIARGESPALGMLEGLALLAAGVLLLIPGFVTDLAAFALLIPPLR
                     RGVIRLYLRRVGLEGTVTRHHPDSARPDRPPLEGESRRED"
     gene            1240..1566
                     /gene="cutA"
                     /locus_tag="AC16FV_03045"
     CDS             1240..1566
                     /gene="cutA"
                     /locus_tag="AC16FV_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR2849074.1"
                     /translation="MKRHEYQLVLTTCPDTEAAERIAQTLVKERLAACVNILPIAKSI
                     YLWKGNVESAAEQLLIIKSMARAFRAIQKRILELHPYELPEVIAVPIADGLPDYLAWI
                     RNPDKT"
     gene            1563..3860
                     /locus_tag="AC16FV_03050"
     CDS             1563..3860
                     /locus_tag="AC16FV_03050"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR2849075.1"
                     /translation="MMKKFLAALLLLLLSPAHAAAPDEELLEADQAFHLSTRTLNSTT
                     LEASWKIAPGYYMYRDKFKFEALDGTQLKNPVFPRGKKKQDPLFGEVETYTKSVKIRI
                     PFVRTEGASTARLRITSQGCNEPVGVCYPPIVKEIDFKLPAGKSKPTAPATAAADKIT
                     SLKDLTHTLAPATGELEPVDPDKAFQVSVMARDNATLLARMDIADCCYLYRDKTKFEL
                     VAADGTPISDVRLGDVTLPPGKTKTDEFIGKTEVYVQGFETALPVTGLAAGDRDLQLK
                     VTYQGCSEKGVAICYPPTTKIFGVHSRGGVLSTVDAAVPPPTAPAGIVTRDMGNLALA
                     VLAAFGAGLLLSFTPCVLPMVPILSGVIVGTEGTHITKLRGGLLSYSYVLGTAITYTV
                     AGAVAGATGEQLQAYFQTPWAIGTFAALLVLLAFSMFGFYQLQVPSSIQSLLHHHSSR
                     MHQQAKQWFGGELIGAFILGLVSALIIGACVSPVLVSVLGAAIAAKDPVLGGGIMFAL
                     AHGQGAILVALGVGAGFLLPKVGKWMDSVKHLFGALLIAVAIYLLGYLPQVPVLFLWA
                     AFFIISGVYLGATQTLPETASGWRYLWKGVGTFLLIWGVLALLGGFAGHRDFLHPLPL
                     SSLSTGMMPGAGSTTAATTDGHLFERVNSLNDLENRLTAAKAAGKPVILDYYADWCTD
                     CLRMEKATFADPRVREELRRRFVLLQADVTDPNNAEGKAIKQRFGVYGPPAMLFFSAD
                     GQERRELRTYGFRDVDEFIALLRKL"
BASE COUNT          771 a         1299 c         1159 g          730 t
ORIGIN      
        1 ccgtacgcca ccgaagacat tgacatcgcc cggcgcgaag cgctggcctt tgaaaaaccg
       61 cctgaacaag gcctgctcga gatgctgaag gactccggaa tccccttcat cgaggtcccc
      121 tcgctcaacc gcaagaaacc accgacctcc ttcaaacaac gcggacgatc gcggtttcac
      181 gtcgacctcc tggtgccgtc ggccaatgaa gacttttccg tggtgcccgt tcccgaactc
      241 aaggcgcatg ccaccggtct gccctacctc tcctatctcc tggccgagtc ccagacggcg
      301 atgctgatgg cacgcgaggg ctgttgcccg gtacgggtgc cgctgcccga gcgcttcgct
      361 gtccataaac tcatcgtctc gcgcctgctc accggacgtg aggcgaaatc ggacaaggat
      421 gtgttccagg cttgcgtgct gtgcgccgca ctggcggaaa gccatcccga cgccatcgaa
      481 tccgccgtgg cgcagatccc cctccgggct aaaaaatacc ttcctgccgc ggtcgagtcg
      541 gcccggcgtt ttcttgagcc cgctcatggg cgcgcttggg aagagctttc gggagccatg
      601 acatcgtaag ggagttcgga ccgggaaatt tatcagttac cggtatagcg ggttcgaaca
      661 agcttcaatc ctcccggcgg gactcccctt ccaatggagg gcggtcaggt ctggcggagt
      721 cggggtgatg ccgggtaacg gtaccctcaa gaccgacgcg gcgcaggtaa agccgaatga
      781 ccccgcggcg cagcggagga atcagcaggg caaaggcggc gaggtcggtg acaaatccgg
      841 ggatcagcaa cagcacgccg gccgccagca aggccaggcc ctccagcatc cccaacgcgg
      901 gcgactcgcc gcgggcgatc atggcatgca cccgcgtcgc agcggcgaag ccctgggtgc
      961 gaaccagcca gacgccaaaa acagcgctgg ccaaaaccag aaacagggtc atgcccacgc
     1021 cgatacgcga gccgacctga ataaataccg cgatctcggc tatcggtatg agcagcaaca
     1081 acagcaaaag catgaaccac gtcctgtgat taaaaccgca gcataacccg gttagccgcg
     1141 gcctcgcatc cggggcgcgt gcgtcgcagt ttcgctacaa ttgagaataa atcggaatcc
     1201 cgcgccgtca tcatggcggg caatggaacg ccaacctgga tgaaacggca cgaataccaa
     1261 ctggtcctta ccacctgtcc cgacaccgag gcagcggaac gtattgcaca aaccctggta
     1321 aaggaacgtc tcgccgcctg cgtcaatatc ctgcccatcg ccaagtccat ttacctgtgg
     1381 aaaggcaacg tggaatccgc cgccgaacaa ctgctgatca tcaagtccat ggcgcgcgcg
     1441 ttccgtgcca tccagaaacg cattctggaa ctgcatccct atgaacttcc cgaggtcatc
     1501 gcggtcccta tcgccgacgg cctgcccgac tatctcgcct ggattcgcaa cccggataaa
     1561 acatgatgaa gaaattcctt gccgccctgt tactcctgtt gttgtcaccg gcgcatgccg
     1621 ccgcgcccga cgaagaactg ctcgaagccg atcaggcctt ccacctgagc acgcgcacgc
     1681 tcaacagcac cacgctcgaa gccagttgga aaatcgcccc cggttattac atgtaccggg
     1741 acaagttcaa attcgaggcg ctcgacggca cgcaactgaa gaatccggtt ttcccgcgcg
     1801 gcaagaaaaa acaggacccg cttttcggcg aggtcgaaac ctataccaag tcggtcaaga
     1861 tacgtattcc gttcgtgcgc accgagggcg cctctacggc gcgcctgcgc atcacctcgc
     1921 agggatgcaa cgagccggtg ggagtgtgtt acccgcccat cgtgaaagag atcgatttca
     1981 aactaccagc gggcaaatcg aagcccaccg cgcctgccac agccgcggcc gataaaatca
     2041 cctccctcaa ggatctcacc cacaccttgg cgcccgccac cggcgagctg gaaccggtgg
     2101 acccggacaa ggcatttcag gtcagcgtca tggcgcgcga taacgccacg ctgctggcgc
     2161 gcatggacat cgccgactgc tgctatctct atcgcgacaa gacgaagttt gaattggtgg
     2221 cggccgatgg cacaccgata tccgatgtac ggctcggcga cgtcacgctg cccccaggca
     2281 aaaccaagac cgacgagttc atcggcaaga ccgaggttta cgtacaaggt tttgagacgg
     2341 cgcttcccgt cacgggcctc gctgcgggcg accgggacct gcaactcaag gtgacgtatc
     2401 agggctgttc ggaaaagggt gtggccatct gctatccgcc gacgaccaaa atcttcggtg
     2461 tgcattcccg tggcggcgtg ttgagcacgg tggacgccgc ggttccgccg ccgaccgcgc
     2521 ccgccggcat cgttacacgc gacatgggca atctcgcgct cgccgtgctt gcggccttcg
     2581 gcgccggatt gttgctgagc ttcacgccct gcgtgctgcc catggtgccg atcctctcgg
     2641 gcgtcatcgt gggcacggaa ggcacgcaca tcaccaagct gcgcggcggt ctgctgtcgt
     2701 acagctacgt gctcggcacc gcgataacct ataccgtcgc cggcgccgtc gccggcgcca
     2761 ccggggaaca gctacaggcc tatttccaga cgccctgggc catcggaact ttcgccgcgc
     2821 ttctggtgct gctggcattc tcgatgttcg gtttctatca attgcaggta ccgtcctcga
     2881 tccagtcgct gctgcaccac cactccagcc gcatgcacca gcaggccaag cagtggttcg
     2941 gcggcgaact gatcggggcg ttcatccttg gtctggtgtc ggcgctgatc atcggcgcct
     3001 gcgtctcgcc ggtgctggtc tcggtgctgg gcgctgccat cgccgccaag gacccggtgc
     3061 tgggcggcgg catcatgttc gcgctggcgc acggtcaggg cgcgatcctg gtcgcgctcg
     3121 gggtcggcgc cggtttcctg ctgcccaagg tgggcaagtg gatggacagc gtgaagcacc
     3181 tgttcggcgc actgctcatt gccgtcgcca tctatctgct cggttacctg ccgcaagtac
     3241 cggtgctgtt cctgtgggcc gccttcttca tcatctccgg tgtctacctc ggcgccaccc
     3301 agaccctgcc cgagactgcg agtggctggc gctatctgtg gaaaggcgtg ggcaccttcc
     3361 tcctgatctg gggcgtgctg gcgctgctgg gtggattcgc cggccatcgc gatttcctgc
     3421 acccgctgcc gctgtcttcc ttatcgaccg gcatgatgcc gggcgccgga agcacaaccg
     3481 cggcaaccac ggacggtcat ttgttcgagc gcgtcaacag cctgaacgat ctcgaaaacc
     3541 ggctgaccgc cgccaaggcc gcgggcaagc cggtcattct cgattactac gccgactggt
     3601 gcaccgactg cctgcgcatg gaaaaggcca cctttgccga cccgcgcgtg cgcgaggaac
     3661 tgcggcggcg tttcgtgctg ctgcaagccg acgtgaccga tccgaataac gcggaaggca
     3721 aggccatcaa gcaacgcttc ggtgtctacg gtccgccggc catgttattc ttttccgccg
     3781 atggacaaga gcgacgggaa ctccgcacct acggctttcg cgacgtggac gaattcatcg
     3841 ccctgttgcg caagctctga agccaggctt tctgcctgac ggatgtcatc cacttttaaa
     3901 aactttctgc tgctgatttc cgcggtattg gcgctgatgg gcggttattg ggcggccca
//
LOCUS       DBNGZE010000120         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_17073, whole genome shotgun sequence.
ACCESSION   DBNGZE010000120 DBNGZE010000000
VERSION     DBNGZE010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_17073"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="AC16FV_03055"
     CDS             <1..1342
                     /locus_tag="AC16FV_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2849076.1"
                     /translation="MLKKETDEASKKRLADLDADIQKLEREFSDLNEIWKSEKAAVHG
                     AQHIKEALDRARVEFETARRAGDLARMSELQYGRIPELEKQLSAAQAAEGKERKLLRN
                     AVTENEIAEVVSRWTGIPVSKMMEGERDKLLRMEEALHNRVVGQDEAIRVVSNAIRRS
                     RAGLSDPRRPYGSFLFLGPTGVGKTELTKALANFLFDSDEAMVRVDMSEYMEKHTVAR
                     LIGAPPGYVGYEEGGQLTEQVRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRLTDGQ
                     GRTVDFRNTVIVMTSNLGSNLIQELAGEENYDKMKNAVMNIVSGHFRPEFINRIDDIV
                     VFHPLGREQLHRIAELQVRYLRERLQAREMDLELTAKALDKLAEAGFDPVFGARPLKR
                     AIQQHVENPLAQEILAGKFGPKDVIEVDVAGDELVFRRKQASARKATASDRAAR"
     gene            1409..2902
                     /locus_tag="AC16FV_03060"
     CDS             1409..2902
                     /locus_tag="AC16FV_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR2849077.1"
                     /translation="MSIDKRSFKVGIIAGIFLVSGILVGIVFSSRLDWLPSATSAPTE
                     TAPLPAIGGTPPNFVPVVKAVMPAVVNISTTRLVKQGGQMPGPFMDDPFFRQFFGEEF
                     FHRFQIPRERRENSLGSGVIVDSAGYIVTNNHVVAKADEIKVLLNDKREFTGKVVGTD
                     PKSDIAVIKISAKDLPVVPWGDSDKLEVGEYVLAIGNPFGLNQTVTQGIVSAVGRANV
                     GIADYEDFIQTDAAINPGNSGGALVNARGQLVGINTAIFSRSGGYMGIGFAVPSNMTR
                     TVMDSLIKGGKVVRGWLGVSIQEVTPDLAKQFGLKESRGALVSEIIPDSPAAAAGIQS
                     GDVITAFNGRTVDSPSTLRNTVAQTPVGKTIKVELLREKKTMSVEVKITEQPKDVAHA
                     DDETVQGDGKNTALAGVEVRNLTSEIARQLGLPPATAGVVIAGVEQGSAAEEAGLQEG
                     DVIMEINRQPVRNTGDFKRLSAKLPKKDSTLLLINRQGRKLFLAIQP"
     gene            2970..>3279
                     /locus_tag="AC16FV_03065"
     CDS             2970..>3279
                     /locus_tag="AC16FV_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HIR2849078.1"
                     /translation="MRLPYSPLPLLVFLFALGFLLAIVQIGALTIAFDKLGLTTNSAF
                     LLLFASLFGSAINLPLFTMTAERPPQPPVLPGPLRRLLRHQLREFKGKTLVAVNVGGC
                     L"
BASE COUNT          660 a         1010 c         1030 g          579 t
ORIGIN      
        1 gatgctgaag aaggaaaccg atgaggcgtc caagaagcgc cttgccgatc tcgacgccga
       61 tatccagaag ctggagcgcg aattctccga tctcaacgag atctggaaat ccgagaaggc
      121 ggcggtgcac ggcgcgcagc acatcaagga ggcgctcgac cgcgcgcgcg tggaattcga
      181 gaccgcgcgc cgcgccggcg atctggcgcg catgtccgag ctgcaatacg gccgcatccc
      241 cgagctggag aaacagctca gtgccgcgca ggccgccgaa ggcaaggaac gcaagctgct
      301 gcgcaatgcc gtgaccgaaa atgaaatcgc tgaggtcgtg tcgcgctgga ccggcattcc
      361 ggtgtcaaag atgatggaag gcgagcgcga caagctgctg cgcatggaag aggcgctgca
      421 caaccgcgtg gtcgggcagg acgaggccat tcgcgtagtg tcgaacgcca tccgccgctc
      481 gcgcgcgggc ctgtccgatc cgcgccggcc ttacggctcg ttcctgtttc tcgggcccac
      541 gggcgtgggc aagaccgaac tgaccaaggc gctcgccaat tttctgttcg acagcgacga
      601 ggccatggtg cgcgtcgaca tgtcggaata catggaaaag cacacggtcg cgcggctgat
      661 cggcgcgccg cccggttatg tcggctatga agagggcggt cagctcaccg agcaggtgcg
      721 ccgcaagccc tattccgtga tcctgctcga tgaagtcgaa aaggcgcatc ccgatgtgtt
      781 caatgtgttg ttgcaggtgc tggacgacgg tcgtctcacc gacggccagg gccgcaccgt
      841 ggatttccgc aacaccgtca tcgtcatgac ctcgaacctg ggctcgaacc tgattcagga
      901 actcgccggc gaagagaact atgacaagat gaagaacgca gtcatgaaca tcgtgtccgg
      961 ccacttccgt cccgagttca tcaaccgcat cgacgacatc gtggtgttcc acccgctcgg
     1021 gcgcgagcag ttgcaccgca ttgccgagct gcaggtccgg tatctgcgcg aacgcctgca
     1081 ggcgcgcgag atggatctgg agctcaccgc caaggcactc gacaagctgg ccgaggccgg
     1141 tttcgatccg gtatttggcg cccgcccgct caagcgcgcc atccagcaac acgtggaaaa
     1201 tccgctggcg caggaaatcc tggcgggcaa attcggcccg aaggacgtca tcgaggtcga
     1261 cgttgctggc gacgagctgg ttttccgccg gaaacaggca tcggccagga aggccacggc
     1321 cagcgaccgc gccgcgcgct gaactttttc gtatttcctc catccatgct catggataga
     1381 atagcccgcg ccgcgggagg gaaaattcat gtcgattgac aagcgctcgt tcaaggtagg
     1441 gatcatcgcc gggatatttc tggtatccgg catactcgtt ggaatcgttt tcagttcgcg
     1501 ccttgactgg ctgccgagcg ctaccagcgc tcccacagaa accgcgccgc tgccggcaat
     1561 cggaggcaca ccgcccaatt tcgttcccgt cgtgaaggcg gtcatgcccg cggtggtgaa
     1621 catttcgacc acgcgcctgg tcaagcaggg cggccagatg ccggggccgt tcatggacga
     1681 tcccttcttc cgccagttct ttggcgagga attttttcac cgcttccaga tcccgcgcga
     1741 acggcgcgag aacagcctcg gttccggcgt gatcgtggat tccgccggtt atatcgtcac
     1801 caacaaccac gtcgttgcca aggctgacga gatcaaggtg ctgcttaacg acaagcgcga
     1861 attcaccggc aaggtcgtgg gtaccgatcc caagtccgac atcgccgtga tcaagatcag
     1921 cgccaaggac ctgccagtgg tgccgtgggg cgattcggac aagcttgaag taggcgaata
     1981 cgtgctggcc atcggcaatc cctttggact caaccagacc gtcacgcagg gcatcgtcag
     2041 cgccgtgggg cgcgccaacg tcggcatcgc cgattacgag gacttcatcc agaccgacgc
     2101 tgcgatcaat ccgggcaact cgggcggcgc gctggtcaac gcgcgcggcc agctggtggg
     2161 catcaacacc gccatcttct cgcgttccgg cggatacatg ggcatcggct tcgccgtccc
     2221 cagcaacatg acgcgcaccg tgatggacag cctgatcaag ggcggcaagg tggtgcgcgg
     2281 ctggctcggc gtctcgatcc aggaagtgac gccggatctg gcgaagcagt tcggcctgaa
     2341 ggagtcgcgc ggcgcgctgg tcagcgagat cattccggac agtcccgccg cggccgccgg
     2401 cattcagagc ggcgacgtca tcactgcgtt taacggcagg accgtggaca gcccgagcac
     2461 tctgcgcaac accgtggcgc agacacccgt cggcaagacc atcaaggtcg aattgttgcg
     2521 cgaaaagaaa accatgtcgg tcgaggtaaa gatcaccgag caaccgaagg acgtcgcgca
     2581 cgcggacgac gaaacggtgc agggcgatgg caagaacacc gcactggcgg gtgtcgaggt
     2641 gcgcaacctg acgtccgaga tcgcgcgtca gcttggtctg ccgccggcaa cggcgggcgt
     2701 ggtcatcgcc ggcgtggaac agggtagcgc cgccgaggaa gccggcttgc aggaaggcga
     2761 cgtcatcatg gaaatcaacc gtcagccggt gcgtaatacc ggcgacttca aacgcctcag
     2821 cgccaagctg ccaaagaagg acagcaccct gttgttgatc aaccgccagg gccggaagct
     2881 tttcctcgcc atacaaccgt gagccgtaag tagtcgtgaa ccgtaaatcg tgagtcgtgt
     2941 tacgattcgc gatttacggt gcatgaacca tgcgtctgcc gtattcaccg ctgccgctgc
     3001 tggttttttt attcgccctc ggcttcctgc tcgcgatcgt ccagatcggg gcgctcacga
     3061 tcgcgtttga caagctgggc ctgacgacga attccgcttt tctgctgctg ttcgcctcgc
     3121 ttttcggcag cgcgatcaac ttgccgctct tcacgatgac agcggaacgc ccaccgcaac
     3181 cgcctgtgtt gccggggccg ctgcggcggc tgctccggca ccagttgcgc gagttcaagg
     3241 gaaaaacact cgtggcggta aatgtgggcg ggtgtctca
//
LOCUS       DBNGZE010000121         1961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_573217, whole genome shotgun sequence.
ACCESSION   DBNGZE010000121 DBNGZE010000000
VERSION     DBNGZE010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_573217"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC16FV_03070"
     CDS             <1..441
                     /locus_tag="AC16FV_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2849079.1"
                     /translation="GCASKFAGSAIAMHIFSLEREQEWDPKHHVEKILGKIAEGDFTV
                     ACWEPGQISPYHCHPHATEIYFCFSGRGTMRTPTESIAVTSGTFVVHPPGEVHEYENG
                     LARTLLFRVRYGPDMRAHHYEWRGNKDWKQSAEDAEYYRRHPVG"
     gene            complement(442..1404)
                     /locus_tag="AC16FV_03075"
     CDS             complement(442..1404)
                     /locus_tag="AC16FV_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR2849080.1"
                     /translation="MKKPNCPIIAIEEHYWDRELAKHFTGPEAGRGNETDERLYDLGS
                     LRLREMDEAGIDIQVLSHGAPSAQKLAPDIAVDLTRQVNDLLANVCAANPKRFAAFAA
                     LPTADPKAAADELERTVTKLGFKGAMLHGMSNGEFLDLKKFWPIYERAEKLDVPIYFH
                     PSLPHPKVTEIYYQDYAKDFPLVVRPAWGYTVETATQAIRLVLSGVFDKHPNLKIILG
                     HFGETLPFLVWRIDASLKRPGQKAMSFRDVFCKNFYVSTIGFLSDPALICCMLEMGVD
                     HILFSVDWPFVMNPPAVKWMESVSISDSDKVKILSGNAKRLLKM"
     gene            1510..1881
                     /locus_tag="AC16FV_03080"
     CDS             1510..1881
                     /locus_tag="AC16FV_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849081.1"
                     /translation="MPHTLLLPNKQFASGALVLTPTSVMHRAMLPILRILPVGGVLLA
                     ILILVLALSPPDGSRSPMAATIAPARGALVDRDRHPEVRQFLILAALKRADELNRLRG
                     LPDTPAYSDNAQSKVAGLPTD"
BASE COUNT          415 a          595 c          549 g          402 t
ORIGIN      
        1 ggctgcgcat caaaattcgc cgggagcgcc atcgccatgc atatcttcag cctcgagcgc
       61 gaacaggagt gggatcccaa gcaccatgtt gaaaagatcc tcggcaaaat agccgagggt
      121 gacttcaccg tggcttgctg ggagcccggc caaatcagcc cctatcattg tcatccgcat
      181 gccaccgaaa tctacttctg cttttccggc cgaggcacca tgcgaacacc gacggaaagc
      241 atcgcggtta cgtccggcac ctttgtcgtt cacccgcccg gcgaggtgca cgaatacgag
      301 aatgggctgg cgcgcacgtt gcttttccgc gtacgttacg gacccgacat gcgcgcgcac
      361 cattacgaat ggcgcggcaa caaagactgg aagcagtctg ctgaagatgc cgagtattat
      421 cggcgtcatc cggtcggctg atcacatctt caaaagtcgc ttggcgtttc cactcaagat
      481 tttgaccttg tcagagtcgg agatcgacac gctttccatc cacttgactg ccggcggatt
      541 catcacgaat ggccagtcga ctgagaacag aatgtggtcc acgcccatct ccagcatgca
      601 gcagatcagc gccgggtcgg ataagaaacc aatggtggac acgtaaaagt ttttgcagaa
      661 gacatcgcga aagctcatcg ccttctgccc cggccgcttg agtgaagcat cgatacgcca
      721 aaccaggaac ggcagcgtct cgccgaaatg gccgagaatg atcttcaggt tcgggtgctt
      781 gtcgaacact ccggagagga ccagccggat cgcttgcgtc gccgtctcca ctgtgtagcc
      841 ccaggccggc cgcaccacca acgggaagtc tttggcatag tcctggtaat agatttccgt
      901 caccttcgga tgcggcagcg agggatggaa gtagatcggc acgtcaagtt tttccgcacg
      961 ctcgtagatc ggccagaatt tcttgaggtc gaggaactcg ccgtttgaca tgccatgcag
     1021 catggcgccc ttaaaaccaa gcttggtgac agtgcgctcg agttcgtcgg cggcggcctt
     1081 cggatcggcg gtcgggaggg ccgcaaaagc tgcgaagcgt ttgggattag ccgcgcacac
     1141 attggcgagg aggtcgttca cctgccgggt cagatctacg gcgatgtcag gcgccagctt
     1201 ctgggcggat ggcgcgccat gcgagagaac ctggatgtcg atgccggcct cgtccatctc
     1261 ccgcaggcgc agactgccta gatcatagag ccgctcgtct gtctcattgc cacgtccggc
     1321 ttcgggacca gtgaaatgct tggccaattc ccgatcccaa taatgctcct cgatggcaat
     1381 gattggacaa ttcggctttt tcatcatgac gcttcctcgc aacgctaatg gaaccgcttc
     1441 gtagcaatcg cgaggtaagc tgtcacggcc ctcccatgac ggtcgtgtgc ggcagcgcca
     1501 gtgatataaa tgccgcacac cttgctgttg ccgaacaaac agttcgcaag cggggctttg
     1561 gtattgaccc cgacctcagt tatgcacagg gcgatgctgc cgattctccg gattctccct
     1621 gtgggcggcg tacttctcgc cattttgatc ctggtgctgg cgcttagccc gccagacgga
     1681 tcccgttccc ctatggctgc aacgatcgcg cccgcacgcg gcgcattagt tgaccgcgac
     1741 cgtcatcccg aagtgcggca attccttatc ctcgctgccc tcaaacgcgc tgatgaactc
     1801 aatcgtttac gcgggctgcc cgacacgccg gcttactccg ataacgcaca atccaaagtc
     1861 gcgggcctcc ccaccgactg aagcgacacg gatccggaag aaacgggctc gattaatgag
     1921 acgcccaggg ctagcattcc agtcgaaatc ggcgagccct c
//
LOCUS       DBNGZE010000122         7769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1406245, whole genome shotgun sequence.
ACCESSION   DBNGZE010000122 DBNGZE010000000
VERSION     DBNGZE010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1406245"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16FV_03085"
     CDS             complement(<1..464)
                     /locus_tag="AC16FV_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR2849082.1"
                     /translation="MDRVMTRAWISLIVLCAIVPRIASAEPDPIVFGVFPRWNAQITV
                     RDFTPLAVRLGHELGRPVRIETDKDFDSFMRRVYAREFDLVHMNQLQYVRAHEAAGYR
                     AIAKLCDNPACTIKAIIVTRRDAKLKKVSDLRGKTIAFGDPTAMVSHVLTKSL"
     gene            complement(618..1883)
                     /locus_tag="AC16FV_03090"
     CDS             complement(618..1883)
                     /locus_tag="AC16FV_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR2849083.1"
                     /translation="MTLAIIALLVLIAALGVPLFVVLGAGSLIATYAADLDPAVLMIE
                     MLRLTSSPNLMAIPLFTLAGVALARGGAPRRLVKLFNASFGWMPGGLAIVALLACAFF
                     TAFSGASGVTILALGGLLYPMLVGENYTKRFSLGLLTSSGSLGLLFPPSLVVLLYGII
                     ANVSIEQLFTAGLVPGTILLVMLALYAMVTSRRLGIPRHPFSPVVFLGAVRRGLWDIL
                     LPVGIIAGLLGGYVTVTEAASCSAAYALLLESVIHRRLNTLTLYYEVFRETCVLVGSL
                     LIILGIALGLTNLLIDAQLPMKILVMLEQDVQSQLQFLLLLNAFLLVIGCIMDIFSAT
                     MVIVPLILPLAQRFGVDPVHLGIIFLANLEIGYLTPPVGINLFLASQRFREPMLNLFR
                     AALPFLLIMLVWLALVTYFPYFSLWWKKI"
     gene            complement(1880..2392)
                     /locus_tag="AC16FV_03095"
     CDS             complement(1880..2392)
                     /locus_tag="AC16FV_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR2849084.1"
                     /translation="MTEPDAHYARNRTWKSKLEFYFTRVESILAISGLALMLGLSLTE
                     IVLRNLFHTSIAGADVLIRHLVLWVSFIGAVVAVRERHIKVEIIAVWLPDRWRRRLER
                     PIFLFSTVVCAIIAWTAVRFWYQEWLNAPADERWIAVLGIVIPLSFLLLALHFALRFL
                     IGPRSIERAA"
     gene            complement(2389..3396)
                     /locus_tag="AC16FV_03100"
     CDS             complement(2389..3396)
                     /locus_tag="AC16FV_03100"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR2849085.1"
                     /translation="MKKLYALLLLIFFFPAAQAQDTYVLKFATLAPQGSTWMNLIADW
                     AKKVEKESQGRLSFKLYPGGVSGDEPDVLRKIRFGQLHGGAMTGHGIGYIYSPTRVLE
                     IPFLFRNYDEIDHVRARLMPEIRDGFRKNGFELLGWMEVGFIQLFSQEPIYSLEDMRK
                     RRIWLWQGDPLGKAFFTASGISPVPLPITEVFTSLSTGLIDTTIAPPLGAIALQWFSK
                     TPYMTEIPVMDGIGGLLVSRQFFDKLPADLQELLRRTGEETGHRLLVETRRDNEKSLK
                     VLKQNGVTFTSEWKDSDAVLYDLRDRAAAALAKNNYIPGDLYARTRQELDRYRSQKGK
                     K"
     gene            complement(3393..4241)
                     /locus_tag="AC16FV_03105"
     CDS             complement(3393..4241)
                     /locus_tag="AC16FV_03105"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR2849086.1"
                     /translation="MSIVLCGLVLNACSMGQMVARSSLSILDSGNIAMNRETDLELAR
                     AAIPANLKLIEGLILELPDNAELRLQAAQGFYGYAYGFIEDDDHRRASELYRRGLEHA
                     LRALAVAGLSADIATLPQKELERQLTTLDHGAVPALFWSASCWAKWIDMNRDDPARIA
                     EMGKAAALMMRVLELDENYYYGGAHLFFGVYYGAKPPMLGGDFERSAQHFEKARAVTQ
                     GKLLFADMLRAQYLARQQLDRRQFHERLAAVIDTPPDVFPEMALANAIARQKAKHLLT
                     KEEEWF"
     gene            4404..6356
                     /gene="yoaA"
                     /locus_tag="AC16FV_03110"
     CDS             4404..6356
                     /gene="yoaA"
                     /locus_tag="AC16FV_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR2849087.1"
                     /translation="MPVTSKDILGPDGPLAGRLEGFTPRRAQQEMAARIEQALVDYSV
                     FIAESGTGTGKTFAYLVPALLSGKKILISTGTRHLQDQLYHRDLPIVRDALAIPVTSA
                     LLKGRSNYLCHHRLERAETEGRLTGRREQSNLALIREWSGRTQRGDIAEMSRIPEDSD
                     LWPQVTSTADNCLGSACSHYDECFVNRARREALAADVVVVNHHLFFADLALREEGFGQ
                     LLPGVEAVIFDEAHQLPEVASNFLGLSLSSHQLASLCRDAIAEDLKEHSGIAGLPPAA
                     QSLEKAIADFRLAFGVEPRRDAWDELENAKAVHAALAELKSRLTDFSAVLDMAAAKGQ
                     GLANCARRASDLLDRLMMISESTPADYIAWFETTARTFTLYLTPLDIAASFRSHTGNG
                     KKAWIFTSATLAIKKSFAHFQARLGLEEAETGLWDSPFDYAQQTLLYIPPGLPAPSAP
                     DYTERVLDAALPVLQASRGRAFILFTSHRALKAAAARLRDKLEYPLLVQGDAPRSELL
                     RRFRDLGNAVLLGTSSFWEGVDVRGEALSCVIIDKLPFASPDDPVLQARAAAMEQAGR
                     SPFTEYQLPEAVITLKQGVGRLIRDENDHGVLVLCDPRLLSKGYGKIFLASLPPMPLT
                     RILQDVEAFFARDNLTRRELREASPA"
     gene            6373..6858
                     /locus_tag="AC16FV_03115"
     CDS             6373..6858
                     /locus_tag="AC16FV_03115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR2849088.1"
                     /translation="MTVPRKLVHFLAFGFGAGKAPVAPGTFGTLVGIVVYLLLQPLSA
                     LAYAITVLALFALGVWLCQMTERDLGVHDHPGIVWDEIVGYLITMFMAPSGWVWIAAG
                     FLLFRLFDIWKPFPIRQIERRIQGGFGNMLDDALAGFYALAIMQAVAYLSRTPAAPWM
                     S"
     gene            6846..7634
                     /locus_tag="AC16FV_03120"
     CDS             6846..7634
                     /locus_tag="AC16FV_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2849089.1"
                     /translation="MDVLIIYLAVGAFAGLIAGLLGVGGGLIIVPVLVFVFQGQGVDA
                     SVIVHLAIGTSLATIAITSISSMRAHHRHGAVQWAVFRRLTPGIVVGALLGAVIADFL
                     PTKAMRLWFGIFELLVAAQIGFNLMVAPRGHLPGTLGTSIAGGVIGAISAALGIGGGT
                     LTTPFLVWCNISLRQAVATSSACGLPIALAGAAGFVATGWHNSALPTWSSGYLYWPAF
                     AGIVISSLLFAPLGAKLTHTLPVTVLRRFFAVFLAVLGIRMLIG"
     gene            7644..>7769
                     /locus_tag="AC16FV_03125"
     CDS             7644..>7769
                     /locus_tag="AC16FV_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR2849090.1"
                     /translation="MELETLARAVSQELKRQGLMLATAESCTGGWVAQIITSVAGS"
BASE COUNT         1509 a         2273 c         2453 g         1534 t
ORIGIN      
        1 agcgatttcg tgagcacgtg actcaccatc gcggtgggat cgccaaaggc gatggttttg
       61 cccctgaggt cgctcacttt cttcagcttc gcgtcgcggc gtgtgacgat aatcgctttg
      121 atcgtgcagg ccgggttgtc gcacaacttt gcgatcgccc ggtagcccgc cgcctcatgg
      181 gcgcgcacgt attgcagctg gttcatgtgc acgagatcga attcccgggc atagacccgg
      241 cgcatgaagg agtcgaaatc cttgtcggtt tcaatgcgta caggccggcc cagctcatgt
      301 ccgagtcgca ctgccagcgg cgtgaaatca cgcaccgtga tctgcgcgtt ccagcggggg
      361 aaaaccccga aaacgatcgg atcgggctcg gcagaagcga tccgtggcac gatggcgcac
      421 aatacgatca ggctgatcca cgcccgggtc attacgcgat ccatgacttg tcacgactgt
      481 accgtaataa gttacggtgc tgccgtgcta cccgtgcgaa tgtctgaaaa tctgcaaata
      541 aggcaggaac tggtcactgc tcatcctcca catcctgagt tatatttcca gctgatgcgg
      601 atatagacgc gggtgcccta tattttcttc caccacaggg aaaaataggg aaagtaggtc
      661 accaaagcca gccaaaccag cataataagg agaaacggca gggcggcgcg gaacaggttc
      721 agcatgggtt cgcggaagcg ctgactggca agaaaaagat taatgccaac gggcggggtc
      781 aggtagccga tctcgaggtt ggcgaggaag atgatgccga gatgtaccgg atcgacaccg
      841 aaacgctgcg ccagcggcag tatcagcggc acgatgacca tggtggcgga aaagatgtcc
      901 atgatgcatc cgatcaccag aagaaaggca ttcaggagca gcaggaattg cagctggctt
      961 tgcacgtcct gttccagcat caccaggatc ttcatcggta gctgggcgtc gatcaggagg
     1021 ttggtcaggc cgagcgcgat gccgaggatg atcagcaggc tgcccaccag cacgcaggtt
     1081 tcgcggaaca cctcataata taaggtcagc gtgttcagcc gacggtgaat cacgctttcc
     1141 agtaacaggg cataggctgc ggagcaggac gccgcctcgg tcacggtcac gtaaccccca
     1201 agcagtcctg caatgatccc gacgggaagg aggatatccc atagccccct gcgaaccgca
     1261 cccagaaaaa caacagggga aaacggatgt cgcggtatgc cgagccgccg ggaggtcacc
     1321 atggcgtaca gcgccagcat gacgagcaat attgttccgg gcaccagacc cgcggtgaag
     1381 agctgttcga tgctgacgtt cgcgatgatg ccgtacaaca acacaaccag gctgggtgga
     1441 aacagcaggc cgagcgatcc ggaggaggtc agcaggccga gcgagaagcg tttcgtgtag
     1501 ttttcgccca ccagcatggg gtagagcaaa ccgccgagcg ccaggatggt gacaccggag
     1561 gcgccggaga aggcggtgaa gaaggcgcag gccagcagcg ccacgatcgc cagcccgccc
     1621 ggcatccagc caaatgaagc attgaacagt ttcaccaacc gtcgcggtgc tccgccacgg
     1681 gccagcgcca cgccggccag ggtgaaaagc ggaatggcca tgaggttggg tgaggacgtg
     1741 agccgcagca tctcgatcat caggacggcc ggatcgagat cggcggcgta ggtggcgatc
     1801 aggctgccgg cgcccaatac cacaaacaac ggcacgccga gcgcggcgat cagaaccagc
     1861 agggcgatga tcgcgagcgt catgccgctc tctcgatcga gcgtggaccg atcagaaagc
     1921 gcagtgcgaa atggagagcc agcaggagaa agctcaacgg aatgacgatg ccgagcacgg
     1981 cgatccatct ctcgtcggcg ggcgcattga gccactcctg gtaccagaag cgcaccgcgg
     2041 tccaggcgat gatggcgcag acaaccgtgg aaaacaggaa gatcggacgt tcgaggcgcc
     2101 gccgccatct gtcaggcagc cagacggcga tgatttccac cttgatgtga cgttcgcgca
     2161 cggccaccac cgcgccgatg aagctgaccc acagcaccag atggcgaatc aggacatcgg
     2221 cgcccgcaat cgaggtatgg aagagattgc gcagaacgat ctcggtcagc gacaggccga
     2281 gcatcaacgc caaccctgaa atcgcgagga tgctttctac gcgggtgaaa taaaactcaa
     2341 gcttggactt ccacgtccgg ttgcgggcgt agtgcgcgtc cggctcagtc acttcttgcc
     2401 cttttgcgag cggtaacgat ccagttcctg gcgcgtgcgg gcgtagagat cgcccgggat
     2461 atagttgttt ttcgcgagcg cggcggcggc gcggtcacgc aggtcataga gtacggcgtc
     2521 actgtccttc cactcgctgg tgaaggtcac gccgttttgt ttcagcacct tgaggctttt
     2581 ctcattgtcc cgtcgcgttt caaccagcag gcgatgaccg gtttcctcgc cggtgcggcg
     2641 cagcagctcc tgaaggtccg cgggcagctt gtcgaaaaac tggcgcgata ccagcagtcc
     2701 gccgataccg tccatcaccg gaatttcggt catgtaaggc gtcttggaaa accattgcag
     2761 cgcgatggcg ccgagcggcg gcgcgatggt ggtgtcgatc agaccagtgg agagactggt
     2821 gaagacttcc gtgatcggca gcggcacggg cgatatgccg ctggccgtga agaaggcctt
     2881 gcccaggggg tcgccctgcc acagccagat gcgccttttc ctcatgtctt caagagaata
     2941 aatgggctcc tgcgagaaga gctggatgaa gccgacttcc atccagccaa gcaactcgaa
     3001 accgttcttg cggaagccgt cgcggatttc cggcatgagc cgcgcgcgca catgatcgat
     3061 ctcgtcatag ttgcggaaca ggaacgggat ttccagaacg cgcgtgggag agtagatgta
     3121 tccgatgccg tgtcccgtca tggcgccgcc atgcaactgg ccgaagcgga ttttgcgcag
     3181 gacgtcgggt tcatcgccgg agacgccgcc ggggtagagc ttgaagctga gccgaccctg
     3241 gctttccttt tccaccttct tcgcccagtc ggcgatcagg ttcatccagg tcgaaccttg
     3301 cggcgccagg gtggcaaact tgagtacata ggtatcttgc gcctgtgcgg cgggaaagaa
     3361 aaaaatcagc aaaagcaggg cgtagagttt tttcaaaacc attcctcttc tttggttagc
     3421 agatgtttgg ctttttgtcg ggcgatggcg ttggccagcg ccatttccgg aaagacatcg
     3481 ggtggggtat cgatgacggc cgccagtcgt tcgtggaact gccgccggtc gagctgctga
     3541 cgcgcgagat attgcgcccg cagcatgtcg gcgaacagca gtttcccctg ggttaccgcg
     3601 cgcgcttttt caaaatgttg ggcggagcgc tcgaagtcgc cgcccagcat gggtggcttg
     3661 gcgccgtaat acacgccgaa aaacagatgc gcgccgccat agtaatagtt ctcgtcaagt
     3721 tcgagcacac gcatcatcag cgccgcggcc ttgcccattt cggcgatgcg cgccggatcg
     3781 tcgcggttca tgtcgatcca cttggcccag caggaagcgg accagaaaag ggcgggtacg
     3841 gcgccgtggt cgagcgtggt gagttgacgc tccagttcct tttgcggcag cgtggcgatg
     3901 tcggcggata atcctgcgac cgcgagcgcc cgcaaggcat gttctagccc acgccggtag
     3961 agttcggacg cgcgccggtg gtcatcgtct tcgataaaac cgtaggcgta accgtaaaat
     4021 ccctgcgccg cctgcagtcg cagttcggcg ttgtctggaa gttccaggat caatccttca
     4081 atgagcttga ggttggccgg aatcgcggcg cgggccagtt ccagatccgt ctcccggttc
     4141 atggcgatgt tgccgctgtc caggatggac aacgaagagc gcgccaccat ctgtcccatg
     4201 gagcaggcgt tcaggaccag accgcagagg acgatcgaca agcttcttat tattagatgg
     4261 gggagcaagg caggttgccc tagagtatga gcagacaggg gtctggattc gacctgcatg
     4321 gttatggtta caccccgagg tattgtatgc ttcgggcagc ttatctccca cttaagtcag
     4381 gcgcaaggag cggaaaaatt tcgttgccgg ttaccagcaa agatattctg ggtcctgacg
     4441 ggccactcgc cgggcggctg gaaggcttca cgccgcgtcg cgcgcagcag gagatggcgg
     4501 cgcgcatcga gcaggcgctg gtggattatt ccgttttcat cgccgagtcg ggcaccggca
     4561 ccggcaagac ctttgcctat ctcgtgccgg cgctgctatc cggcaagaag attctgatct
     4621 ccactggcac gcgccatctg caggaccagt tgtatcaccg cgatctcccg attgtgcgcg
     4681 acgccctcgc gataccggtc acctcagcat tgctcaaggg ccggtccaat tacctgtgcc
     4741 accaccgtct cgagcgcgcg gaaacggaag ggcgactgac cggccggcgc gagcagtcga
     4801 acctggcgct gatccgcgag tggagcgggc gcacgcaacg cggcgatatc gccgagatga
     4861 gccgcatccc cgaggattcc gatctgtggc cacaggtgac ctccaccgcg gataattgtc
     4921 ttggcagcgc ctgttcccat tacgacgaat gtttcgtcaa ccgcgcgcgc cgcgaggccc
     4981 tggccgccga cgtggtggtg gtcaatcatc acctgttctt cgccgatctg gccctgcgcg
     5041 aggaaggttt cggtcaattg ctgcccggtg tcgaggcggt cattttcgac gaggcgcatc
     5101 agctgccaga ggtggcgtcg aactttctcg gtctttcact cagcagtcat cagctcgcca
     5161 gcctgtgtcg tgatgccatt gccgaagatt tgaaggagca cagcggcatc gctggcttgc
     5221 cgcccgccgc gcagtcgctc gaaaaggcca tcgccgattt tcgtctggcg tttggcgtgg
     5281 agccgcgccg cgatgcctgg gacgagctgg agaatgccaa ggcggtacac gcggcgctgg
     5341 cggagctgaa atcgcgcctg acggattttt ccgcggtgtt ggacatggcc gctgccaagg
     5401 ggcagggact ggccaattgc gcccgccgcg cctcggatct gctggaccgg ctgatgatga
     5461 tcagcgagag cacgccggcg gattacatcg cctggtttga aaccacggct cgtactttta
     5521 cgctgtatct cacgccgctc gacatcgcgg cttccttccg gagccacacc ggcaatggca
     5581 agaaggcctg gatattcact tccgccacgc tcgcgataaa gaaaagtttc gcgcattttc
     5641 aggcacgact gggtctggag gaggccgaaa ccgggttgtg ggacagtccc ttcgactacg
     5701 cacaacagac ccttttatat attccacccg gactgccggc gccatcggcg ccggattata
     5761 cggagcgcgt gctggatgcg gctttgccgg tgctgcaagc tagccgcggc cgcgcgttca
     5821 ttctgttcac cagtcatcgc gcgctcaagg ccgcggccgc ccgactgcgc gataaattgg
     5881 agtatccact gctggtgcag ggtgacgccc cgcgctcgga attgctccgg cgtttccgcg
     5941 acctgggcaa tgccgtgctg ctcggcacca gcagtttctg ggaaggtgtg gacgtgcgcg
     6001 gcgaggcgct gtcgtgcgtg attatcgata aactcccttt cgcttcaccc gacgatccgg
     6061 tgttgcaggc gcgcgcggcg gcgatggagc aggccggacg cagccccttt accgaatatc
     6121 agttgccgga agccgtgatt acgctgaagc aaggcgtcgg ccgcctgatt cgcgacgaga
     6181 acgaccatgg cgtgctggtg ctgtgcgatc cgcgcctgtt gagcaagggc tacgggaaaa
     6241 tatttctcgc cagcctgccg cccatgccct tgacccggat cctgcaagat gtcgaagcat
     6301 tcttcgcgcg cgacaatctc acccgccggg aattgcgcga ggcgtcaccg gcgtgatgtc
     6361 gcagattcgc tcatgaccgt gccgcgaaag cttgtccatt ttctcgcctt cggcttcggc
     6421 gccggcaagg cgccggtggc gccgggtacc ttcggcacgc tggtcggaat cgtcgtgtac
     6481 ctgttgctcc agcctctgtc cgcgctggcg tatgccatta cggtgctggc gctgtttgcg
     6541 ctgggcgtgt ggttgtgcca gatgacggaa cgggacttgg gcgtacacga tcatcccggc
     6601 atcgtgtggg atgaaatcgt cggatacctt attaccatgt tcatggctcc gagcggatgg
     6661 gtctggatcg cggcaggatt tctcctgttc cggttgttcg acatctggaa gccgtttccc
     6721 atccgccaga tcgaacgccg gattcaggga ggattcggca acatgctcga cgacgccctc
     6781 gcgggttttt atgccttggc gattatgcaa gccgtggcat atctgtccag gaccccggct
     6841 gcaccatgga tgtcttgatc atttatctgg cggtcggtgc cttcgccggt ctcatcgccg
     6901 gcctgctcgg tgttggaggc gggcttatta tcgtcccggt attggtattt gtatttcagg
     6961 ggcagggtgt cgatgccagc gtgattgtgc atctggcaat cgggacttcg ctcgcgacca
     7021 tcgccatcac ttcgatctcc tccatgcgtg cgcatcaccg gcacggcgcg gtgcaatggg
     7081 ctgtattccg ccggctgacg cccggcatcg tggtcggggc gctcctgggc gcggtgatcg
     7141 ccgatttcct gccaacgaaa gcgatgcgcc tgtggttcgg cattttcgaa ttactggtgg
     7201 cggcgcagat cggcttcaat ctcatggtgg cgccgcgcgg gcacttgccc ggcacgttgg
     7261 gtacgagcat tgcgggcggt gtcatcggag cgatatcggc ggcgctgggt attggtggcg
     7321 gtaccctgac gacgccattc ctggtgtggt gcaacatctc gctgcggcag gcggtggcaa
     7381 cttccagcgc ctgcggcctg cccatcgcgc tggcgggcgc ggccggtttc gtcgccactg
     7441 gttggcataa cagcgccctt cccacatgga gcagcggtta tctgtactgg ccggcgttcg
     7501 ccggtatcgt gatatccagt ctgttgtttg ccccgctcgg cgcgaaactc acgcacaccc
     7561 tgccggtcac cgtgctgcgc cggttctttg cggtttttct cgccgtgctt ggaatacgaa
     7621 tgttgatcgg gtaaaacgcc ttcatggaac tcgaaaccct cgcacgcgcc gtgagtcagg
     7681 aactgaaacg ccagggcctc atgctggcca cggcggaatc ctgcaccggc ggctgggtgg
     7741 cgcagataat cacctcggtg gcgggcagc
//
LOCUS       DBNGZE010000123         2359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_156128, whole genome shotgun sequence.
ACCESSION   DBNGZE010000123 DBNGZE010000000
VERSION     DBNGZE010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_156128"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..270
                     /locus_tag="AC16FV_03130"
     tRNA            194..270
                     /locus_tag="AC16FV_03130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:228..230,aa:Met,seq:cat)
     gene            443..928
                     /gene="rimP"
                     /locus_tag="AC16FV_03135"
     CDS             443..928
                     /gene="rimP"
                     /locus_tag="AC16FV_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR2849091.1"
                     /translation="MRGTRADLRKLLEPGVVALGFELVDVELAGSHHSPTLRVYIDGP
                     AGINVDDCARVSRQLSALLDVEDPLPGQYTLEVSSPGLDRPLVTPEDFRRFIGETIKV
                     KMQPPLLGRKNFSGRLVDVTADHVVVEVDNEHFDLAFDGMERARLVPRFRTSGSDGRA
                     R"
     gene            925..>2359
                     /gene="nusA"
                     /locus_tag="AC16FV_03140"
     CDS             925..>2359
                     /gene="nusA"
                     /locus_tag="AC16FV_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR2849092.1"
                     /translation="MIGNEVLMIVDAVSREKGVDKEIIFQALEAALATATRKRHKGDT
                     DARVVIHRDTGSYDTFRRWEVIADETETVEFPDRQIKLTEARVQKPDIQLGEFIEAPL
                     EPVEFGRIAAQAAKQVIMQKVREAEREQIVNLYKDRKGEMVTGVVKRLERGDVVVDLG
                     NAEALLAKSAMIPREGLRPGDRIRALLEDVKSLPRGPQLFLSRVAPKLLIELFKLEVP
                     EAGEGLITIHGAARDPGLRAKIAVSSSDPRIDPIGACVGMRGSRVQSVSNELAGERVD
                     IINWSDNPAQYVINSLAPAEVQSIVVDEESHSMDVIVEEKQLSQSIGRSGQNVRLASE
                     LTGWELNIMTEQSAQEKGTKEAANAIQLFMEQLSVDENVATVLVQEGFSSLDEIAYVP
                     KQELLAIEEFDDELVDELRNRARDLLLTRAIAQEEKISLAEPAKDLLEMQGMDENTAH
                     LLASHGIKTMEELAEQAVDDLLEIEGMP"
BASE COUNT          467 a          698 c          778 g          416 t
ORIGIN      
        1 gcggtgtcat ggggtggatc gcctacatct actgtatcga ggtgctgcgg gagcgaccgc
       61 cacaggccgg cagcgcgaat tctcctgatc tttagcgcag acagctgttg gcagtgaagg
      121 gcgcgcccgc ccgataagtt cctatttttc cttgaataat gccattccgg acggtagact
      181 tcgcggtcct tgctgcgggg tggagcagtc tggcagctcg tcgggctcat aacccgaagg
      241 tcgcaggttc aaatcctgcc cccgctacca aatcccaaag gccccgctgg ggcctttttg
      301 tttcacggca gaccgacggc accggccgcg gcactttccg attccggggg aagtgcttat
      361 aattcaaccc ggtttcagcc gtctcaagag taagtataag aagtgggccg cgcgcccact
      421 tttgttttat ggggcagaag ttatgcgtgg tacgcgagcg gatctgcgaa agctgctcga
      481 gcccggtgtc gtggccttgg gcttcgagct cgtggacgtg gaactggcgg ggagccacca
      541 tagccccacg ttgcgggtgt atatagatgg ccccgcgggc atcaacgtcg acgactgtgc
      601 gcgcgtcagc cggcaacttt ccgcgctgct cgacgtcgag gacccgctgc cggggcagta
      661 caccctggag gtgtcttcgc ccggcctcga tcggccgctg gtgacgccgg aggacttccg
      721 gcgcttcatc ggcgagacga tcaaggtgaa gatgcagccg ccgctgctgg ggcggaagaa
      781 tttttccggc cggctggtgg atgtgacggc ggatcacgtc gtggtggaag tggacaatga
      841 gcattttgat ctggcgttcg atggcatgga gcgggcccgg ttggtccccc gattccggac
      901 gtcaggcagt gacgggagag cgagatgata ggtaacgaag tattgatgat tgtcgatgcc
      961 gtgtcgcgcg aaaagggcgt ggacaaggaa atcatttttc aggcgctcga ggcggcgctc
     1021 gctactgcca cgcgcaaacg ccacaagggc gacaccgacg cgcgcgtcgt cattcaccgc
     1081 gacaccggat cctacgacac cttccgtcgc tgggaagtga ttgccgacga aaccgaaacg
     1141 gtcgagttcc ccgaccgcca gatcaagctg accgaggcgc gcgtccagaa gccggacatc
     1201 cagctcggtg aattcatcga agcgccgctc gagccggtcg agttcggccg catcgccgcg
     1261 caagccgcca agcaggtcat catgcagaag gtgcgcgagg cggagcgcga gcagatcgtc
     1321 aacctctaca aggaccgcaa gggcgaaatg gtcacgggcg tcgtcaaacg cctggagcgc
     1381 ggcgacgtcg tggtggacct cggcaatgcc gaggcgctgc tggccaagag cgccatgatt
     1441 ccccgcgaag ggttgcgccc cggagaccgc attcgcgcgc tgctggaaga cgtgaaatcc
     1501 ctgccgcgcg gaccgcagct gtttctctcg cgcgtcgcgc ccaagctgct gatcgagctt
     1561 ttcaagctcg aggttcccga ggccggcgaa gggctgatca ccattcacgg cgccgcgcgc
     1621 gatcccggcc tgcgcgccaa aatcgcggtc agttcctccg acccgcgcat cgatcccatc
     1681 ggcgcctgcg tcggcatgcg cggttcacgc gtgcagagcg tgtcgaacga actcgccggc
     1741 gagcgcgtcg acatcattaa ctggtcggat aatccggcac agtacgtgat caattcgctc
     1801 gcgccggccg aagtccagtc catcgtggtg gacgaggaat cgcacagcat ggatgtgatc
     1861 gtggaagaaa aacagctttc ccagtcgatc ggacgcagcg gacagaacgt gcggctggct
     1921 tccgagctca ccggctggga gctgaacatc atgaccgagc agtcggcgca ggaaaaaggc
     1981 accaaggagg cggcgaacgc catccagctc ttcatggaac agctttcggt ggatgagaac
     2041 gtggccacgg tcctggtgca ggaaggtttc tccagcctgg acgagatcgc ctacgtgccg
     2101 aagcaggaat tgctggccat cgaggagttc gatgacgaac tggtggacga actccggaac
     2161 cgggcgcgcg atttgctgct gacccgtgcc atcgcccagg aagagaagat cagtctggcg
     2221 gaaccggcca aggacctgct ggaaatgcag ggcatggacg agaacaccgc gcatctgctg
     2281 gcgtcccatg gcatcaagac catggaagag ctggcggagc aggccgtgga cgacctgctc
     2341 gagatcgagg gcatgcccg
//
LOCUS       DBNGZE010000124         3827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_17601, whole genome shotgun sequence.
ACCESSION   DBNGZE010000124 DBNGZE010000000
VERSION     DBNGZE010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_17601"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="AC16FV_03145"
     CDS             <1..1108
                     /locus_tag="AC16FV_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HIR2849093.1"
                     /translation="RHGVRKVVKSKSMVTEECSLNDYLASVGVEVLETDLGEYILQLA
                     HEPPSHIVAPVVHKSKEEIADLFEEKHGRPRTTQIAEMCREAREVLRPGFLSADMGIS
                     GANFIIAESGSVLIVTNEGNGRLTTTLPRVHVAITGIEKVVPTLEDVSTLLRLLPRSA
                     TGQSITNYVSVLTGTRGKDESDGSEHFHIILLDNGRSRLLGTELQPMLRCIRCGACMN
                     HCPVYQNIGGHAYGWVYPGPMGSVLTPSFVGLENAIDLPNASTFCGECAVVCPVKIPL
                     PDLMRHLRHQQVERHLRPWTERAALRLWSWTARHPAVYALFTRVAVRLLAWAGGKNKR
                     LRHLPGVSGWTRGRDMPAPSGRTFRELYQARRSA"
     gene            1105..1734
                     /locus_tag="AC16FV_03150"
     CDS             1105..1734
                     /locus_tag="AC16FV_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR2849094.1"
                     /translation="MSARENILARIGKARGSSIAADVDGEIRKHRRGPIPPLGDDLVK
                     RFAERAVSLASDVVQARTITEIPGLVARYLSERQLPMQGVCWPALAPYGWQSTGIELQ
                     ARAARGDDLVGVTGAFAGIAETGTLMLLSGPETPATVSLLPETHVAVLESGRIVATME
                     QAWDRLRVERGTLPRAVNFVSGPSRTADIEQTVTLGAHGPYRVFIIIVG"
     gene            1757..3259
                     /locus_tag="AC16FV_03155"
     CDS             1757..3259
                     /locus_tag="AC16FV_03155"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR2849095.1"
                     /translation="MSQQLLSLWINGKPQTSLSARTAEVFNPASGEVIRRVPLASKQD
                     VEKAVAAAQAAFPGWRDTTPLRRARILMRFRELMQAHRDELIHLTTEEHGKTREDAAG
                     SLQRGIEVIEFAMGVPHLLKGEKAEDVGRGVDCHSLLQPVGVCAGITPFNFPVMVPLW
                     MFPVAIACGNTFILKPSEKVPSASLRMAELLREAGLPDGVFNVVPGDKEAVDALLTHP
                     DVRAISFVGSTPVAKYIYETAARHGKRVQALGGAKNHAVVMPDADLDFAADALMGAAY
                     GSAGERCMAISTVVAVGAAGDALVAKMREKAARLHVGPGDQQGIDMGPVITAAHRERI
                     RKLIDSGVAEGAKLVLDGRGLKVTGREKGFYLGPTLFDKVTTTMEIYREEIFGPVLIV
                     LRADNIDAAINLVNSNPYGNGTAIFTRSGAYARRFERDIEVGMVGINVPIPVPMAFFS
                     FGGWKASLFGDLHVHGMEGVYFYTRSKVVTSRWPGGEEQGSSNLSMPTLG"
     gene            3275..>3827
                     /locus_tag="AC16FV_03160"
     CDS             3275..>3827
                     /locus_tag="AC16FV_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HIR2849096.1"
                     /translation="MTMPTDHRKNLLKIFQSALAAVNGRACVRERLKERPLPGKVYMI
                     AVGKAACAMAQGAQDVMGGNIADALIITKKGHAEALPWPVLEAGHPLVDESSLEAGRK
                     LLAFTDRIPPDASVLVLISGGASALVEALPPQISLDRFQEINRWVLGSGLDINDYNRI
                     RRRLSRLKGGRLAKLLSPRRVLCL"
BASE COUNT          674 a         1133 c         1285 g          735 t
ORIGIN      
        1 gcgccacggc gtgcgcaagg tggtcaagtc gaagtccatg gtcacggagg aatgttcgct
       61 caacgattac ctggcgagcg tgggcgtgga ggtgctggaa accgatctgg gcgagtacat
      121 tctgcaactg gcgcatgaac cgccgtcgca catcgtggcc ccggtggtgc acaagagcaa
      181 ggaggagatc gccgacctgt tcgaggaaaa gcacgggcgt ccacgcacca cgcagatcgc
      241 cgagatgtgc cgcgaggcgc gcgaggtgtt gcgccccgga tttctctcgg ccgacatggg
      301 gatttccggc gcgaatttca tcattgccga gtccggctcg gtattgatcg tcaccaacga
      361 gggcaatggc cggctcacca ccacgctgcc gcgcgtgcac gtggccatca ccggcatcga
      421 gaaagtggtg ccgacgctgg aggatgtcag cacactgctg cgcctgctgc cgcgttccgc
      481 caccggtcag tccatcacca attacgtctc ggtcctgacc ggaacccggg gcaaggatga
      541 atccgacggg tcggaacatt ttcatattat tttgctggac aacggtcgca gccgcctgct
      601 cggcaccgaa ctgcaaccga tgttgcgttg tatccgttgc ggcgcctgca tgaaccactg
      661 tccggtgtat caaaatatcg gcggccatgc ctatggctgg gtctatccgg gtcctatggg
      721 ctcggtgctg acgccatcct ttgtcgggct ggaaaacgcc atcgacctgc ccaatgcttc
      781 aactttctgc ggcgaatgcg ccgtggtctg tccggtgaag attccgttgc cggacctgat
      841 gcgccacctg cgccatcaac aggtggagcg ccatctgcgg ccgtggaccg agcgcgccgc
      901 gctcagactg tggtcgtgga ccgcgcggca tccagcggtg tacgcactgt tcacgcgcgt
      961 ggcggtgcga ttattggcct gggccggtgg caaaaataaa agattgcgcc atctaccggg
     1021 cgtctccggt tggacgaggg ggcgcgacat gcctgcgcct tccggccgca ctttccgcga
     1081 gctgtatcag gcgaggcgtt ccgcatgagc gcacgcgaga atatcctggc gcggatcggc
     1141 aaggcgcgcg gttccagtat cgccgccgat gtcgatggcg aaatccgcaa gcatcggcgc
     1201 ggaccgatcc cgccattggg agacgatctg gtcaaacggt ttgccgagcg ggccgtgagt
     1261 cttgcgagcg atgtggtgca ggcgcgcacg atcactgaaa taccgggcct ggtggcgcgc
     1321 tacctgagcg agcgccagtt gccgatgcag ggtgtatgct ggccggcgct ggcaccctat
     1381 ggctggcaat ccaccggcat cgagttgcag gcgcgcgcgg cgcgtggcga tgatctggtg
     1441 ggcgtcaccg gcgcttttgc cggcattgcc gagaccggca ccctgatgct gctctccggc
     1501 ccggagacac cggcgacggt cagcctgttg ccggagacgc atgtcgccgt gctcgagtcc
     1561 gggcgcatcg tggcgaccat ggagcaggcc tgggaccgct tgcgtgtcga gcgcggtaca
     1621 ctgccgcgcg cggtgaattt cgtttccggc ccttcgcgca cggccgatat cgagcagacc
     1681 gtgacgctcg gcgcgcatgg gccctatcgc gtattcatca ttatcgtcgg ttgacattct
     1741 tcagagaggt gccgccatgt cacaacagct tttatcgctt tggatcaatg gcaagccgca
     1801 gacgagcctg agcgcgcgca cggcggaggt tttcaatccg gcgagtggcg aggtcatccg
     1861 gcgcgtgccg ctggccagta aacaggatgt ggaaaaagcg gtggcggcgg cgcaggcggc
     1921 gtttcccggt tggcgtgaca cgacgccgct gcggcgcgcg cgcattctca tgcgctttcg
     1981 cgagctgatg caggcacatc gcgacgaact gattcatctg accacggaag aacatggcaa
     2041 gacgcgcgag gacgccgccg gctcgctgca gcgcggtatc gaggtcatcg agttcgccat
     2101 gggcgtgccg catctgctga agggcgagaa ggcggaagac gtagggcgtg gcgtggattg
     2161 ccattcgctc ttgcagccgg tgggcgtgtg cgccggcatc acgccgttca attttcccgt
     2221 gatggtgccg ttgtggatgt tcccggtggc gattgcttgc ggcaatacct ttatcctcaa
     2281 gccttcggag aaagtgccct cggcgagcct gcgcatggcc gaattgctgc gcgaggccgg
     2341 attgccggac ggcgtattca acgtggtccc cggcgacaag gaggcagtgg acgccctgct
     2401 gacgcatccg gacgtgcgtg cgatttcctt cgtcggttcg acgcccgtgg cgaaatacat
     2461 ctacgaaacc gcggcacgtc atggcaagcg cgtacaggcg ctgggcgggg cgaagaacca
     2521 cgccgtcgtc atgccggatg ccgatctcga ttttgccgcc gacgcgctca tgggcgcggc
     2581 ttacggttcg gccggcgagc gctgcatggc gatctcgacg gtcgtggccg tgggcgcggc
     2641 cggcgatgcc ctggttgcga agatgcgtga aaaagccgcg cgactgcatg tcggtccggg
     2701 cgatcagcag ggtatcgaca tggggccggt gattaccgcc gcacatcgtg aacgcatacg
     2761 caagctgatt gacagcggtg tcgcggaggg cgcgaagctg gtattggatg ggcgcgggct
     2821 caaggttacc ggtcgggaaa agggttttta tctcggacca accctgttcg acaaggtcac
     2881 gacgaccatg gaaatttacc gggaagaaat tttcggtccg gtgctgatcg tcctgcgggc
     2941 tgacaacatc gacgctgcca tcaatctcgt gaacagcaat ccctatggta acggtacagc
     3001 catcttcacg cgctcgggcg cctacgcgcg ccgcttcgag cgcgacattg aggtcggcat
     3061 ggtcggcatc aacgtgccga tcccggtgcc gatggcattt ttctccttcg gcggctggaa
     3121 ggcgtcgctg ttcggtgacc tgcatgtaca tggcatggaa ggcgtgtatt tctatacccg
     3181 cagcaaagtc gtcacgagcc gctggccggg cggcgaagaa cagggttcgt ccaatctgtc
     3241 gatgccgacg ctcggttaac atgccattca tcgcatgacc atgccgaccg atcaccggaa
     3301 aaatctgttg aaaatatttc agtccgcgct cgccgcggtc aacggccgcg cctgcgtgcg
     3361 tgaacgactg aaggagaggc cgctgcccgg aaaggtatac atgatcgcgg tcggcaaggc
     3421 cgcttgcgcc atggcgcagg gcgcgcagga cgtgatgggt ggcaatatcg ctgatgcgct
     3481 gattatcacc aaaaaggggc atgccgaagc gctgccctgg ccggtgctgg aggcggggca
     3541 tccgctggtg gacgaatcca gtctggaagc cggccggaag ctgctggcgt ttaccgaccg
     3601 gattccgccg gatgcctcgg tgctggtgtt gatctccggc ggcgcgtcag cgctggtgga
     3661 ggccttgccg ccgcagatca gtctcgaccg gtttcaggaa atcaatcgct gggtgctcgg
     3721 ttcgggactg gacatcaacg attacaatcg cattcgccgg cgcctgtcgc gtctcaaggg
     3781 cgggcgtctg gccaaactgc tttctccgcg acgtgtactg tgtctcg
//
LOCUS       DBNGZE010000125         4160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_990222, whole genome shotgun sequence.
ACCESSION   DBNGZE010000125 DBNGZE010000000
VERSION     DBNGZE010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_990222"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="AC16FV_03165"
     CDS             <1..319
                     /locus_tag="AC16FV_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HIR2849097.1"
                     /translation="ARKQWLAGQLRAMGKLRLDAGAVKVIREGGKSLLPVGVKAVEGA
                     FTRGEIVSCLDPEGREVARGLVNYSSEEAGRIIGKASDKIESILGYVDEPELIHRDNM
                     VVL"
     gene            complement(383..646)
                     /gene="rpsT"
                     /locus_tag="AC16FV_03170"
     CDS             complement(383..646)
                     /gene="rpsT"
                     /locus_tag="AC16FV_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR2849098.1"
                     /translation="MANTAQAKKRARQAEVHRSRNSGQRTELRTQIKKVRTAVASKDK
                     SAAQQAFREAASVIDRLVGKGLVHKNAAARYKSGLNQQLRTLA"
     gene            941..2515
                     /gene="murJ"
                     /locus_tag="AC16FV_03175"
     CDS             941..2515
                     /gene="murJ"
                     /locus_tag="AC16FV_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR2849099.1"
                     /translation="MSRKLLKSTAVTGGMTLLSRISGLVRDVLFASLIGAGTGVAADA
                     FYVAFRIPNFLRRIFGEGAFSQSFVPVFAEFKTKYSADETRAFVDHMAGILGAILFVV
                     TLVGVIAAPLLVMALAPGFLDEPNKYGLTVQMLRITFPYIFFISLVAMAAGILNTYGR
                     FGAAAFTPVLLNLSMIGAAVWLAPRMDEPVLALAWGVFIAGVVQLLFQIPFLRGIKML
                     PRPCLRLRERHHGVSRVFKLMLPAIFGVSVAQINMLVNTLLASFLITGSVSWLYYSDR
                     LMEFPLGVFGIALATVILPSLARRHANNSREDFSHLLDWGLRWVFLIAVPASVALVIL
                     SGPLLATFFYFGKFSINDVHMSANALMAFSFGLLGFILIKVLAPGFYARQDTKTPMRI
                     GVISLVVNILLSLLLVIPLKHVGLALAISLAAFVNAGVLFLTLRRHDVYRPSPGWFVF
                     LARVSLASVVMGAVLVWGAGDLDSWLRAPALERATRLSIWVITGAVVYLLTVTAFGVR
                     LAQLLLRKGKGAKFDE"
     gene            2508..2924
                     /locus_tag="AC16FV_03180"
     CDS             2508..2924
                     /locus_tag="AC16FV_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HIR2849100.1"
                     /translation="MNSAAQIYQISACNNPRVFTLSEAEELFPLMWKITEEAYRELEP
                     VRRALEAMVPSNPQVRKVEKQYEAIVRRWMGKMERLGLVVKGLWLADFDTGDGYLCWK
                     FPELRIGHYHSYHEGFGGRRALQDIIEELDPDWAHL"
     gene            3008..3979
                     /gene="ribF"
                     /locus_tag="AC16FV_03185"
     CDS             3008..3979
                     /gene="ribF"
                     /locus_tag="AC16FV_03185"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR2849101.1"
                     /translation="MEFIRGWHNLKPRHRGCVATIGNFDGVHLGHQAVIGQLAEKGQE
                     LGLPTQVILFEPQPQEFFQPESAVPRLTRLREKLQALRRYSVDRVLCLHFDAEFAALE
                     PEQFIQRLLVDGLQVRYLVVGDDFRFGKRRRGDFAMLVDAGRKHGFQVVNMHTVTIDG
                     ERVSSTRIRAALAAGDLVLAEKLLGRPYRMCGRVAHGDKLGRTLGFPTANIYLHRKVT
                     PIKGIFAVEVFGLKTEPIAGAANIGTRPTVGGTRTLLEVHLLDFSDDIYGAYVHVNFL
                     RKFRDEKRFDSLDELHRWIEKDVVDVRAFFQDYHPARGKNASREKTA"
     gene            4001..>4160
                     /locus_tag="AC16FV_03190"
     CDS             4001..>4160
                     /locus_tag="AC16FV_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849102.1"
                     /translation="MNDYKNTLNLPQTDFPMKANLATREPEVLRRWQEIDLYRKLREQ
                     GRGRPKYIL"
BASE COUNT          745 a         1144 c         1305 g          966 t
ORIGIN      
        1 ggcgcgcaaa cagtggctgg cggggcagtt acgcgccatg ggaaagctca ggctcgatgc
       61 gggcgcggtc aaggttatcc gcgaaggtgg caagagtctg ttgccggtgg gtgtgaaggc
      121 cgtggaaggc gcattcacgc gcggcgaaat tgtttcctgt cttgaccccg aaggtcgtga
      181 agtcgcgcgc ggattggtga attacagctc ggaagaagcc gggcgtatca tcgggaaggc
      241 cagtgacaag attgaatcga ttctgggcta cgtggatgag ccggaattaa tccacagaga
      301 taatatggta gtgctgtaac gggcacacaa aaacaaaaag gccagcattg ccggcctttt
      361 tgtttaatga atgccgaaga gtttacgcca gcgtgcgcag ttgctggttg agcccgctct
      421 tgtaacgcgc ggcggcgttc ttgtgaacca gtcccttgcc gaccaggcga tcgattacgg
      481 aagcggcttc acggaaggcc tgctgtgcgg cggacttgtc cttggaagca acggcagtgc
      541 gaaccttttt gatctgggtg cgcagttcgg tgcgctgacc ggaattgcgc gagcgatgta
      601 cctcggcttg gcgggcgcgt ttctttgcct gtgcagtgtt ggccaaaaca attctcctga
      661 aaaagcctcg aaagggcggc aactatgctg attttgtgtg tatttgtcaa tcccgcttgg
      721 gggctgtctc tcggtttttc acggagcggc agcggtttgg gaggcccggc acatcctgta
      781 tgatcgcgct ctcatcccga tcagctacaa gccatccgtg gcgcagcaac atgacctcga
      841 tagagtcccc agcccggcca tccgtggccg ggcgctcagc ggcgcagaga cacgttaagt
      901 gtccctgcga caactccgct tcgcccacta aagagcatcc atgagtcgca agctgcttaa
      961 atccacggcc gtcaccggcg gcatgaccct gttgtcgcgg atcagcggcc tcgtccgcga
     1021 cgttctgttc gccagcctca tcggcgccgg taccggcgtg gcggccgatg ccttctacgt
     1081 cgccttccgt atccccaatt tcctgcgccg tattttcggc gagggcgcgt tttcccagag
     1141 ttttgttccg gtgttcgccg agttcaagac taaatactcg gccgacgaga ccagggcctt
     1201 tgtcgatcac atggcgggca tacttggcgc catactcttt gtcgtgacgc tcgtcggcgt
     1261 gattgccgcc cctctcctgg tgatggcact cgcgcctggc tttctcgacg agccgaataa
     1321 atacggcctg acggtgcaga tgctgcgcat caccttcccg tatattttct tcatttcgct
     1381 ggtagcgatg gcggcgggca tacttaatac atatggccgt ttcggcgccg ctgcattcac
     1441 gccagtgctg ctcaatctga gcatgattgg cgccgccgtg tggctggccc cacgcatgga
     1501 cgagccggtc ctggcgctgg cgtggggcgt attcattgcc ggcgtggtgc agttgctgtt
     1561 ccagattccg ttcctgcgtg gaatcaaaat gctcccgcgc ccgtgtttgc gcctgcgcga
     1621 gcgtcaccat ggcgtgtcgc gcgtattcaa gctgatgttg cccgcgatct tcggtgtatc
     1681 ggtggcgcag atcaacatgc tggtgaatac cctgctggcc tcgtttctga tcactggcag
     1741 cgtctcgtgg ctgtattatt ccgaccggtt gatggaattc cctctgggcg tattcggcat
     1801 cgcgctggcg acggtaattc ttcccagtct ggcgcgacga cacgccaaca attcgcgcga
     1861 ggatttttcg catctgctcg actggggact gcgctgggtc tttctgatcg ctgtgccggc
     1921 ctcggtggcg ctcgtaatcc tttccgggcc attgttggcg actttttttt atttcggaaa
     1981 gttcagtatt aacgatgtcc atatgagcgc caatgcactc atggcatttt cattcggtct
     2041 gctgggattc attctgatca aggtgctcgc gccgggcttc tatgcgcgcc aggatacgaa
     2101 aacgcccatg cgcatcggcg ttatctcgct agtggtcaat attctgctga gcctgttgct
     2161 ggtgattccg ttgaagcacg tcgggttggc gctggcgatt tcgctggcgg cgtttgtcaa
     2221 tgccggcgtg cttttcctca cactgcgccg gcacgacgta tatcgtcctt ctcccggatg
     2281 gtttgtgttt ctggcgcgcg tcagtctcgc gagtgtcgtg atgggcgcgg tgctggtctg
     2341 gggcgcgggt gatctggatt cctggttgcg ggccccggcg ctggaacggg caacccgctt
     2401 gtcaatatgg gtaattaccg gcgcagtagt gtatttattg accgtaaccg ctttcggagt
     2461 acgattggca cagttactgc ttaggaaggg gaagggggca aaattcgatg aatagcgccg
     2521 ctcagatata ccagatttcg gcctgcaaca atccgcgcgt gttcacgctg tcggaggccg
     2581 aagaattgtt cccgctgatg tggaaaatta ccgaagaggc ctatcgcgag ctggagccgg
     2641 tgcgccgcgc gctcgaggcc atggtgccga gcaatcccca ggtacgcaag gtggaaaaac
     2701 agtacgaagc gatcgtgcgg cgctggatgg gcaagatgga gcggctcggg cttgtggtga
     2761 aagggctgtg gttggcggat ttcgacaccg gcgacggtta tctgtgctgg aaatttccgg
     2821 agctgcgtat cggccactac cacagttatc acgagggctt cggcgggcgc cgtgccttgc
     2881 aggatatcat cgaagaactc gacccggact gggcgcatct gtagatttcc cgctgttcct
     2941 cgcttgttct cgcgcgcgcg agacggtaac cttcccggtt ttcccgggct tgaatctgaa
     3001 cagttccatg gagttcatac gcgggtggca taacctgaag ccgcggcatc gcgggtgcgt
     3061 cgccacgatc ggaaattttg acggcgtgca tctcggtcac caggcggtga tcggccaact
     3121 ggcggagaaa ggccaggagc tcggtttgcc cacccaggtg atcctgttcg agccgcagcc
     3181 gcaggagttt tttcagccgg aatccgctgt accacgtttg acgcgcctgc gcgaaaaact
     3241 ccaggcactg cgccgctact cggtggatcg cgtgctgtgc ctgcacttcg atgctgagtt
     3301 tgccgccctg gagcccgagc agttcattca gcgcctgctg gtggatgggt tgcaggtgcg
     3361 ctatctcgtt gtcggtgatg atttccgttt cgggaagcgg aggcgcgggg actttgccat
     3421 gctcgtggat gccggccgca aacacggctt ccaggtcgtg aacatgcata ccgtgacgat
     3481 tgacggtgag cgcgtttcga gcacgcgcat tcgtgcggcg ctggcggccg gcgacctggt
     3541 gctggcggaa aagctgctgg ggcggcccta ccgcatgtgc gggcgcgtgg cgcatggcga
     3601 caagctcggc cgtaccctcg gatttccgac cgcgaacatc tacttgcatc gcaaggtgac
     3661 accgattaag gggatcttcg ccgtggaggt gttcggactc aagaccgagc cgattgccgg
     3721 cgcggccaat attggcacgc gtcccacggt tggcgggacg cgcacgctgc tggaggtgca
     3781 tctgctggat ttcagcgatg acatctacgg cgcctatgta cacgtgaatt ttttacgcaa
     3841 gtttcgcgat gagaaacgct tcgattcact ggatgagttg caccgctgga ttgaaaaaga
     3901 tgtcgtggat gtgcgcgctt ttttccagga ctaccatccc gcgcgcggca agaatgcctc
     3961 ccgggaaaag acggcatgag ttttctgcaa agggctcgtg gtgaacgact ataaaaatac
     4021 gctgaatctt ccgcagacgg atttccccat gaaggccaac ctggcgacgc gtgagccgga
     4081 ggtcctcagg cgttggcagg aaatcgatct ctataggaaa ctgcgcgagc aggggcgcgg
     4141 ccgtccgaaa tatatcctgc
//
LOCUS       DBNGZE010000126         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_504569, whole genome shotgun sequence.
ACCESSION   DBNGZE010000126 DBNGZE010000000
VERSION     DBNGZE010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_504569"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="AC16FV_03195"
     CDS             <1..1154
                     /locus_tag="AC16FV_03195"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR2849103.1"
                     /translation="RPKGPLVWLHGASVGELASVLPLIDRIRARRISMLVTTGTVTSG
                     GLAEQRLPRGVIHQFIPLDIPRFTRRFLSHWRPDLALFVESELWPNMMIEASARGIPM
                     VLVNARLSKNSFERWRRLPNSIADLLRRLDLCLPGTSSDAARLSELGASNVVTTGNLK
                     LDVPAPPADPAVLASLQDAIAGRPVIAAASTHAGEEGVVIDVHNRLCSNFPGLLTLIA
                     PRHPERGPGVAEIATAAGLGAALRSRGELPKRTTQIYVADTVGELGLIYRLAPAVFIG
                     GSLVKHGGQNPVEAAKLRTAILHGPHVWNFAEIYAALDNAHGAEVIDDVDRLTAYFAA
                     WLADPDACTRVADAGHNVVENLGGALDRTMASLDPYLMQLQLRQRARNA"
     gene            1243..>1510
                     /locus_tag="AC16FV_03200"
     CDS             1243..>1510
                     /locus_tag="AC16FV_03200"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR2849104.1"
                     /translation="MAMKGAPAGVPVLCVGNFTLGGAGKTPTVMMLARMLADAGERPV
                     CLSRGYGGNVAGPMLVDAHTAVAAEVGDEALLIARVAPTIVARDR"
BASE COUNT          246 a          469 c          477 g          318 t
ORIGIN      
        1 cgcgaccgaa aggccctctg gtctggctgc acggcgccag cgtcggggag ctggcgagcg
       61 ttctgccttt aatcgatcgc attcgtgcgc gtcgcatcag catgttagta acgactggca
      121 cggtcacttc gggcgggctc gccgaacaac gccttccgcg gggggtgatc catcaattca
      181 tccctctcga tattccgcgc ttcacacgta ggtttctcag tcattggcgg ccggatctcg
      241 cattgttcgt cgaatccgag ctttggccca acatgatgat cgaggcatca gcgcgcggca
      301 taccgatggt tctagtcaac gcccgcctgt cgaaaaattc attcgaacgc tggcggcgtt
      361 tgccgaattc gatcgccgac ctgcttcgac gactggatct ttgtcttccc ggcacctcgt
      421 ccgatgctgc gcggctgagt gaactgggcg cgtcaaatgt tgtcactacg ggcaacctca
      481 agcttgacgt gccggcacca cccgccgacc cggcagtgct tgcatcgtta caggatgcaa
      541 ttgccggccg gccggtgatt gcagccgctt ccacccatgc cggcgaagag ggcgtggtga
      601 tcgacgtgca taatcgcctg tgctcgaatt ttcccggcct gctgacgctc atcgcgccgc
      661 gtcatccgga acgcggcccc ggcgtcgccg agatcgcgac tgccgccggc ttgggcgcgg
      721 cactgcgctc gcgcggggaa ttacccaaac gcacaacgca gatttatgtg gcagacacgg
      781 tcggcgagct cggcctgatc tatcggctgg caccagctgt gttcattggt ggctcgctgg
      841 tcaagcacgg cgggcaaaat ccggtcgagg cggctaagct gcgcactgcg atcctgcacg
      901 gtccgcatgt ttggaatttc gctgagatat atgccgcgct cgacaacgcc catggcgctg
      961 aggtgattga cgacgtcgac cgccttaccg cgtattttgc cgcctggctt gctgaccccg
     1021 atgcctgtac ccgtgtcgcc gacgctggcc ataatgtggt ggagaatttg ggtggcgcgc
     1081 tcgaccgtac catggcgtcg ctcgaccctt acctgatgca actccaattg cgccaacggg
     1141 cccgcaatgc gtgacccatc cttctggtgg cacagggctg gccttgccgc aggcttgctg
     1201 gcgccattgg ggctcgtcta tggcgcggtc gcggcgagac gcatggctat gaaaggtgcg
     1261 cccgccggag tgccggtttt atgcgtgggc aatttcacgc ttggcggcgc gggtaagacg
     1321 ccaacggtca tgatgctcgc acgaatgctc gcggacgctg gcgagcgtcc cgtgtgtctc
     1381 agtcgcggct atggcgggaa tgtcgcaggt ccaatgcttg tggatgctca tacagccgtt
     1441 gccgccgaag ttggcgacga ggcgcttttg atcgcgcgtg tggcgccgac catcgtcgca
     1501 cgggatcgcg
//
LOCUS       DBNGZE010000127         2571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_921240, whole genome shotgun sequence.
ACCESSION   DBNGZE010000127 DBNGZE010000000
VERSION     DBNGZE010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_921240"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="AC16FV_03205"
     CDS             complement(<1..576)
                     /locus_tag="AC16FV_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR2849105.1"
                     /translation="MNAVAVRQKALDVVVAVLQREDGRVLLAQRPAGKPWEGYWEFPG
                     GKIESGEAADHALARELHEELGVDPDRVYPWVTQEYAYPEKYVRLHFYRVLAWHGQLH
                     GREGQSMSWENPAAINIGPLLPANDKVLRALSLPSVYAITNAKKYGEAEFMPRLKAAL
                     EKGVRLIQVREHEMPPEQLESVTRRVVTLAHE"
     gene            complement(573..1613)
                     /gene="pyrC"
                     /locus_tag="AC16FV_03210"
     CDS             complement(573..1613)
                     /gene="pyrC"
                     /locus_tag="AC16FV_03210"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR2849106.1"
                     /translation="MQITLTRPDDWHLHLRDGAALRAVLPHTAQRFARAIVMPNLKAP
                     VTTVTQALAYRERILAALPPGARFEPLMTLYLTERTTLEEIAAAKKSGFVHAVKYYPA
                     GATTNSESGVTDLARCRAVFAAMEKHDLPLLLHGEVTDPAVDIFDREQVFIERHLAPL
                     VRDFPKLRVVLEHVTTRQAVEFVKQAPANVVATITAHHLLHNRNAMFQGGLRPHYYCL
                     PVLKREEHRAALVKAATGGNPKFFLGTDSAPHARRAKESACGCAGIYTAHAALELYAE
                     VFEAAGALKQFEAFASHFGPDFYRLPRNRDSITLVKESWAVPGEMAFGDDTLVPLRAG
                     ETVTWKLAVKSS"
     gene            complement(1770..2267)
                     /gene="purE"
                     /locus_tag="AC16FV_03215"
     CDS             complement(1770..2267)
                     /gene="purE"
                     /locus_tag="AC16FV_03215"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR2849107.1"
                     /translation="MSINPRVAIIMGSQSDWEVMQTAVKVLKDFGVSCEARVMSAHRT
                     PDLVAEYVRAAPTRGTKCIIAAAGGAAHLAGVVAAHTTLPVLGVPMPSKHLQGMDSLL
                     STVQMPKGIPVATFAIGEAGAGNAALFAVALLALEDAVLARKLTEFRNKQADAIKVTK
                     LPDIS"
     gene            complement(2283..2571)
                     /locus_tag="AC16FV_03220"
                     /pseudo
     CDS             complement(2283..2571)
                     /locus_tag="AC16FV_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992736.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
BASE COUNT          482 a          810 c          767 g          512 t
ORIGIN      
        1 ctcgtgggcc aacgttacca cgcggcgtgt gactgactcg agttgctccg gcggcatttc
       61 gtgctcgcgc acctgaatga gccgcacgcc tttttccaga gcggctttca gccgtggcat
      121 gaattccgcc tcgccgtatt ttttggcatt cgtgatcgcg taaaccgaag gcagagacag
      181 cgcgcgtaat accttgtcgt tcgccggcag caagggaccg atattgatcg ccgcgggatt
      241 ctcccacgac atgctctgcc cttcgcgtcc gtgcagctgg ccgtgccatg ccagcacgcg
      301 gtaaaagtgc agccgcacat atttctcggg gtaggcatat tcctgcgtca cccacggata
      361 cacccggtcg ggatcgacgc cgagttcctc gtgcagctcg cgcgcgagcg cgtggtccgc
      421 cgcctcgccg ctctcgattt tccctccggg gaattcccag taaccctccc acggcttgcc
      481 cgccggacgc tgcgccagca gcacgcgtcc atcctcgcgc tgcaggaccg cgacgaccac
      541 atcgagcgcc ttctggcgta cggccacggc gttcatgacg atttcaccgc cagcttccag
      601 gtcacggtct cgccggcgcg caacggcaca agggtgtcgt cgccgaatgc catttccccc
      661 ggcaccgccc aggattcttt caccagcgtg atgctgtcgc ggttgcgcgg taaacggtaa
      721 aaatccgggc caaagtgact ggcgaacgcc tcgaattgtt tcaacgcacc agcggcttca
      781 aaaacttccg catacagttc gagcgcggca tgcgcggtat aaatgccggc gcagccgcag
      841 gccgactcct tggcgcggcg cgcatgcggc gcactgtcgg tgccgagaaa gaatttcggg
      901 tttccgccgg tggcggcctt caccagcgcc gcgcggtgtt cctcgcgctt caacaccggc
      961 aggcagtagt aatgcggccg caacccgccc tggaacatgg cgttgcgatt atgcagtaaa
     1021 tgatgcgcgg tgatggtggc cacaacattg gccggcgctt gtttgacaaa ttcaacggct
     1081 tggcgcgtgg tgacatgctc aagcacgacg cgaagcttcg gaaagtcacg cacgagcggc
     1141 gccagatgcc gttcgatgaa aacctgttcg cgatcgaaga tatccaccgc cgggtccgtg
     1201 acctcaccgt gcagcaacag gggcagatcg tgcttttcca tcgcggcaaa caccgcgcga
     1261 caacgggcca gatcggtaac gccgctctcg gagttcgtgg tggcgccggc cggataatat
     1321 ttgaccgcgt gcacgaagcc gctttttttc gcagcggcga tttcctccag cgtggtgcgt
     1381 tcggtgaggt aaagcgtcat aagcggctcg aagcgcgcgc ccgggggcag cgccgcgaga
     1441 atgcgctcgc gataggccag ggcctgggtc accgtggtca ccggcgcttt gagattgggc
     1501 atgacgatgg cgcgcgcgaa ccgctgcgcc gtgtgtggca acaccgcgcg cagggccgcg
     1561 ccgtcgcgca ggtgcaggtg ccagtcgtcc gggcgtgtca gcgtgatctg catgggaaat
     1621 tcttagagtt cccaacaaaa aatagacagg attaacagga tttttcagga tttacaggat
     1681 taaggatttt taaagttaag aaataatcct gttaatcctg tttttaatcc tgtaaatcct
     1741 gttaaatttt tcttccccga tacgtccggt cacgagatgt ccgggagctt ggtaacctta
     1801 atcgcgtcag cctgcttgtt gcggaattcc gtgagcttgc gcgccagcac ggcgtcttcg
     1861 agcgcaagca gggccacggc gaacagcgct gcattgcccg cgccggcctc gccgatggcg
     1921 aaggtcgcga ccgggatacc cttgggcatc tgtaccgtcg aaagtagcga atccatgcct
     1981 tgcagatgct tgctcggcat cggcacgccc agcaccggca gggtcgtgtg cgcggccacc
     2041 acgccggcca gatgcgcggc cccaccagcg gcggcgatga tgcatttcgt gccgcgcgtg
     2101 ggcgcggcgc gcacgtattc ggccacgaga tcgggcgtac gatgcgccga catgacgcgg
     2161 gcctcgcagc tgacgccaaa atccttgagt actttcacgg cggtctgcat cacctcccag
     2221 tcactctggg aacccatgat tatcgccacg cgtggattga tgctcatcaa aatatctcct
     2281 tgttattttc cggatttttg ctgctcgccc gcggcgcgtc ttgcgatagc ttctagaata
     2341 ccacgcatca gatcgagagg cgtcggcggg caaccgcgaa taaccgcatc caccggaatg
     2401 acattcgcta ccgctccaca tgaagcgtag ctcacaccga attcaccgcc gtttgcgcca
     2461 cactcaccga ccgcgatgac cagtttcggt tccggcgtcg catcgaagtg cggcgcagcg
     2521 ccgcttccat gtggcgcgac accggtccgg tgacgagcag catgtcggca t
//
LOCUS       DBNGZE010000128         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_712957, whole genome shotgun sequence.
ACCESSION   DBNGZE010000128 DBNGZE010000000
VERSION     DBNGZE010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_712957"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC16FV_03225"
     CDS             complement(<1..713)
                     /locus_tag="AC16FV_03225"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR2849108.1"
                     /translation="MLEGLIALPWWGYIIAALILTHITIAAVTLFLHRHQAHRALELH
                     PIVSHFFRFWLWLTTGMVTKEWAAIHRKHHAKVETPDDPHSPIQAGIRTVMWQGAELY
                     RREAENAETMEKYGHGTPDDWLERNLYTRHSGKGIALMFALDLLLFGPIGITIWAVQM
                     AWIPIFAAGIINGVGHYWGYRNYEVADASTNIVPWGILIGGEELHNNHHTFGSSAKFS
                     SKWWEFDIGWLYINLMQAVG"
     gene            830..1270
                     /locus_tag="AC16FV_03230"
     CDS             830..1270
                     /locus_tag="AC16FV_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR2849109.1"
                     /translation="MPAPKTPALTTDIIIELRDRPDVPIVLIRRKNPPHGWALPGGFV
                     DVGEAVEHAAVREAEEETALRVTLKILLGCYSDPRRDPRSHTASVVYVAEAEGEPRAQ
                     DDAAGVAIFSPEALPSPLVFDHAQILRDYLTWRQQGRLPFPGTP"
     gene            complement(1161..2543)
                     /locus_tag="AC16FV_03235"
     CDS             complement(1161..2543)
                     /locus_tag="AC16FV_03235"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR2849110.1"
                     /translation="MHELLEVFPSREVVRDFLNSPSTWLQFGIFVAGLLVAKLVAGWL
                     QPRLQTIAQPGVIDSVRRTAVRSGALALVPLLLWLWLLAAATILRNQDLATGLLRPAM
                     VLAGALAAIRMGVFVLRHSFSPGGPLKTWEGTLTVTIWTLVALHILGWLPFVEQMLDE
                     YALSFGKVRISLLNVVSFVLSVAIMLLVALWLANTIRNRVRRSAVLEDSMKIALSKLI
                     RFTLLFLAVLVAMMMAGIDITAFAIFGGALGVGLGLGLQRVVSNFVSGFILVFEGSIR
                     PGDVISFGNTFGTVRALHTRHVVIRTRDGMDILVPNENLMTSDITNWSYEGDRRIRLR
                     LPVQISYDDDPEQAIARLERIAQEHDRVLKDPAPAAVLTGFGDNDVNLELLVWIDDPE
                     RGGDVRSDLFKHIWKDFKTAGIALPFPQRDIYIKEFQGTAVSPADATSDSRAGSARGR
                     KREAKARPPG"
     gene            2685..>3350
                     /locus_tag="AC16FV_03240"
     CDS             2685..>3350
                     /locus_tag="AC16FV_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR2849111.1"
                     /translation="MSILSETQFHNEEAAYKFVEARVWPTGPVCPHCGCFERVGKMGG
                     KSTRIGTYKCYDCRKPFTVKIGTIFESSHVPLRLWLQAIFLIASSKKGVSANQLHRTL
                     GVTLKTAWFMGHRIREAMKADGLEVFGRNGGAVEVDETFIGHDKTIKPKGVKKGRDFH
                     HKNKVLSLIDRETGRSRSIVVDDIKASTLAPLIRANVAKEAKLMTDEASFYTLVGREF
                     AAHG"
BASE COUNT          720 a          966 c         1039 g          625 t
ORIGIN      
        1 ccgacggcct gcatcaggtt gatatacagc cagccgatgt cgaactccca ccacttggag
       61 gaaaacttgg cggaactgcc gaaggtatga tgattgttgt gcagctcctc gccgccgatg
      121 aggatacccc acgggacgat gttggtgctg gcgtcggcga cctcgtaatt gcgatagccc
      181 cagtaatggc cgaccccatt gatgatcccg gcggcgaaga tcggaatcca ggccatctgc
      241 acggcccaga tcgtgatgcc gatgggaccg aatagcaaga ggtcgagcgc gaacatcagg
      301 gcgatgccct ttccgctgtg acgcgtgtac agattgcgct ccagccagtc atccggcgtg
      361 ccgtgaccgt acttctccat ggtttcggcg ttctcggctt ccctgcggta gagctccgcg
      421 ccttgccaca tgacggtgcg gatgccggcc tgtatcgggc tatgcgggtc atccggtgtt
      481 tccaccttgg cgtggtgctt gcgatggatt gccgcccact ccttggtgac catgccggtg
      541 gtgagccaca gccagaagcg gaagaagtgg ctgacgatgg gatgcagctc gagcgcccgg
      601 tgggcctggt gacggtgcag aaaaagggta acagcggcga tggtgatgtg ggtcaggata
      661 agtgcggcga tgatgtatcc ccaccatggg agagcaatca gtccttcaag cattcgttaa
      721 aacctcgttt cattaggcgg tgtgttgtgc aagtatactc gaaacaggcg ctttccgcgc
      781 gtgatcgggc agaaaagccg aaaaacgcgc cggataacga gaaaagatca tgccggcacc
      841 gaaaactccc gcactgacca cggatatcat catcgagctc agggaccgtc cggatgtccc
      901 gattgtgctg atccggcgca agaatccgcc gcacggttgg gcgctgcccg gcggtttcgt
      961 ggatgtgggc gaagcggtgg agcatgccgc cgtgcgcgag gccgaggagg aaacggcgct
     1021 gcgtgtgacg ctcaagatat tgctggggtg ttattccgat ccgcggcgcg atccgcgcag
     1081 tcatacggct tcggtggttt acgtcgccga ggcggaaggc gagccgcggg cgcaggacga
     1141 tgccgcgggc gtggcgatat tcagcccgga ggccttgcct tcgcctctcg ttttcgacca
     1201 cgcgcagatc ctgcgcgact atctgacgtg gcgtcagcag gggagactgc cgttccctgg
     1261 aactccttga tgtaaatgtc gcgctgcggg aagggcagtg caatgccggc agtcttgaaa
     1321 tccttccaga tgtgtttgaa gagatccgaa cgcacatcgc cgccgcgttc agggtcgtca
     1381 atccacacga gcaattccag atttacgtca ttgtctccaa agccggtcag cacggcggcg
     1441 ggcgcggggt ccttgagcac gcggtcgtgt tcctgtgcga tgcgctcaag acgcgcgatg
     1501 gcctgttccg ggtcgtcgtc gtaactgatc tgaacgggca ggcgcagccg gatacggcga
     1561 tcaccctcgt aagaccagtt ggtgatgtcg gaggtcatga ggttctcgtt gggtacgagt
     1621 atgtccatgc catcgcgcgt gcgaatcacg acgtggcgcg tgtgcaacgc cctgaccgtg
     1681 ccgaaggtgt tgccgaaact gatgacatcg cccgggcgga tcgatccctc gaagacaaga
     1741 ataaaaccgc tcacgaaatt actgaccacg cgttgcagcc ccagaccgag accgacgccg
     1801 agcgcgcctc cgaaaatcgc gaatgcggtg atatcgatgc ccgccatcat catggcgacg
     1861 agcaccgcca gaaacagcaa cgtgaagcgg atgagcttgg acagcgcgat cttcatgctg
     1921 tcctcaagca cggcgctgcg ccgcacgcgg ttgcgtatgg tattggccag ccacagggcg
     1981 accagcagca tgatcgcgac ggacaacacg aaactcacca cgttaaggag agaaatgcgc
     2041 accttgccga agctgagcgc gtattcgtcc agcatctgct cgacgaatgg cagccagccg
     2101 agaatatgca gcgcgaccag cgtccagatc gttaccgtca gcgtgccttc ccaggtcttg
     2161 agcggccccc cggggctgaa gctgtggcgc agcacgaaca cgcccatgcg gatcgccgca
     2221 agcgccccgg cgaggaccat ggccggccgc agcaggccgg tagccaggtc ctgattgcgc
     2281 aggatggtgg ccgcggcgag cagccacagc cacaacagca ggggcaccag ggccagtgcg
     2341 cccgagcgca cggcggtgcg gcgcacgcta tcgataacgc cgggctgggc gatggtttgc
     2401 aggcgcggct gtaaccagcc ggcgaccagt ttggctacca aaagtccggc gacgaaaatg
     2461 ccgaattgca accaggtgga tggcgaattc aggaaatcgc gaacgacctc tctcgaggga
     2521 aagacttcca gcagttcatg catgaattga tgttatgtcc gcggaacgcc ggcagcgcgc
     2581 tggcggctag gatagcgagt cgcccggcca aaaaaaaggc tatattatac ttgacgtacc
     2641 caaaataaaa gcgcatactg ttttcatcgt ataggagaac agtcatgagc atcctttccg
     2701 aaacccaatt ccataacgaa gaagcggcct acaagttcgt agaggcgcgc gtctggccga
     2761 ctggtccggt atgtccgcat tgcggctgct ttgaacgtgt cggcaagatg ggcggcaaga
     2821 gcacccgcat aggcacctat aagtgctatg actgccgcaa accgtttacc gtgaagatcg
     2881 ggaccatctt cgagtccagc catgttccgc tgcgactctg gctgcaagcc atcttcctga
     2941 ttgcatccag caagaagggt gtcagcgcca accagctgca tcgtaccctt ggcgtcaccc
     3001 tcaaaaccgc ttggttcatg ggtcacagaa tccgcgaagc catgaaggcg gacggccttg
     3061 aagtgttcgg gcgcaatggc ggcgcggtgg aagtggatga aacctttatc ggccatgaca
     3121 aaaccatcaa gcctaaaggc gtgaaaaagg ggcgcgactt tcaccataaa aacaaggttc
     3181 tgtccctcat tgaccgcgag accgggcgct ctcgcagcat cgtggtggac gacatcaagg
     3241 ccagcaccct tgcccccttg attcgcgcca atgtcgccaa ggaagccaaa ctgatgaccg
     3301 atgaggcttc tttctatacc ttggttggtc gtgagttcgc cgcgcatggc
//
LOCUS       DBNGZE010000129         7918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_365850, whole genome shotgun sequence.
ACCESSION   DBNGZE010000129 DBNGZE010000000
VERSION     DBNGZE010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_365850"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="AC16FV_03245"
     CDS             complement(<1..397)
                     /locus_tag="AC16FV_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849112.1"
                     /translation="MTVEKGSGFTAGQIAAVLSITPRAVRMRAATEGWAHQEQKTRGG
                     ITRLYPLDRLPADVQAAIMNPSSPSRADSLPVGTGLCPAGNTAGFFSAVQESTNVAGA
                     GCAGATSINREELWAQFARRPARQKERARR"
     gene            complement(400..729)
                     /locus_tag="AC16FV_03250"
     CDS             complement(400..729)
                     /locus_tag="AC16FV_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849113.1"
                     /translation="MSLRALISRCLNMLAYRIKPGERERRYRSQRARLERNPDLYRKC
                     KRDSQRAYRERNRDKVTELNRLYRERNRDRINERQRARRDAARRKAGISMSHLFEGLD
                     NRIKSGD"
     gene            1023..1451
                     /locus_tag="AC16FV_03255"
     CDS             1023..1451
                     /locus_tag="AC16FV_03255"
                     /inference="COORDINATES: protein motif:HMM:NF018697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2849114.1"
                     /translation="MSIPEQKQNTPQNPAFLARLKRLIGVEAPYSWAKRMGISKGAFT
                     RIYKEGTVPTARLLERIRKGTGVSLDWLLRGEGAMYSDRVAEEAGPYKAEKGVGPLSK
                     DEELLVRKYRELNPSDKTHFQAIIVKVASTKGVGKQERKK"
     gene            1503..1826
                     /locus_tag="AC16FV_03260"
     CDS             1503..1826
                     /locus_tag="AC16FV_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR2849115.1"
                     /translation="MWFLGLIIWLLLCALAGSVADKKGRAGWVYFAISLFLSPLVGLL
                     LVLLLSPIKENIEKQQIESGQNRKCPFCAEIIKAEAKTCKHCGKDSPPSLPGPKFDPD
                     TGAMI"
     gene            1828..2349
                     /locus_tag="AC16FV_03265"
     CDS             1828..2349
                     /locus_tag="AC16FV_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849116.1"
                     /translation="MTPDKREPVVIYNPKQDQFLTRNRGCLESLFWLCLIGIALFWLF
                     GCAPATVQGLRENHAGKITFDVDENYQSVYRKVLTPARDCWQSGLITAQMVVQGDLYT
                     DTWRGNVTVALHSGAGVDTYFTVDVEALSEAKTRVTVYHAFRNQERAAQAVELWVKQN
                     SKLCRVPGPQEEL"
     gene            2425..3051
                     /locus_tag="AC16FV_03270"
     CDS             2425..3051
                     /locus_tag="AC16FV_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017638.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HIR2849117.1"
                     /translation="MRDRFDICLDFVLPEEIGPFKATAGYISVARAAQIGDTGSETIA
                     GVSRVHNPDWPGWAIVDSRKTEPDFPKNLNLDLPRNAELKRLVGEVYRVKYWQGFRCG
                     EMPAGLDLALFDAAVQHLPKPAAVLFQQAVQAKPDGDIGDLTVKAAGYANVPRALFTY
                     FVRRTEFYMDIILADSRQAKNRSGWFSRVFRLQAYILTTTPIVWGGNP"
     gene            3048..3530
                     /locus_tag="AC16FV_03275"
     CDS             3048..3530
                     /locus_tag="AC16FV_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849118.1"
                     /translation="MNHEMLAQELTLFAPGLATALAEDTTDALATKAVETARYVTGVY
                     SPDMAVTAIKNDPAAQERFRKAMAYLLTVETRARAPEAPAPAAVGEKDLLQSKVTWGV
                     LVPIIAMLTSRYGLDLGDQQGWVDAISGLVGALLIFWGRVAAIKRVTSIAGVKIDAKP
                     "
     gene            3517..3873
                     /locus_tag="AC16FV_03280"
     CDS             3517..3873
                     /locus_tag="AC16FV_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849119.1"
                     /translation="MPNRKQAFLSFFVALLALALAACAAVPQPRSFTDQVTIARALLA
                     GVDKSIGDLTEAGVLHSSKAQQLLDKAKQARRDIEQAETLYRTGKQVDAQNLLATTQT
                     VLLTLREQLMKMEKQP"
     gene            3870..4082
                     /locus_tag="AC16FV_03285"
     CDS             3870..4082
                     /locus_tag="AC16FV_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849120.1"
                     /translation="MTEAGLIKVLDLMIVGSNIGMNINAQLEKFRLLVSQSKTDEEIA
                     EIFKQDLAAAEVEAQAKIDAAKRLGR"
     gene            4087..4500
                     /locus_tag="AC16FV_03290"
     CDS             4087..4500
                     /locus_tag="AC16FV_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849121.1"
                     /translation="MKDVSQRLVEAERERDRLRFRVRYGVWPPVGLPPSEWLKHDQKW
                     PKDMNALERLQVENEVLREEVMMLMRGNQSDEIIPAVIVSALDFGARFSASLYSLPPG
                     HQVHLARDLSTAVQAALDELERLRPGSLGKSASHG"
     gene            4493..4732
                     /locus_tag="AC16FV_03295"
     CDS             4493..4732
                     /locus_tag="AC16FV_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HIR2849122.1"
                     /translation="MADAADYAQAIAEEERKDALAARAARTFHEEPRVVNGQRLCRDC
                     DEPLSKKRLKFVPEAVRCVDCQNLLEQRQRRKKLS"
     gene            4770..5114
                     /locus_tag="AC16FV_03300"
     CDS             4770..5114
                     /locus_tag="AC16FV_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849123.1"
                     /translation="MQIVISWEMVGTAVTILLALFGLLVGIIRWLLDRYVDAIKSQIE
                     IMAKQITAQAGDITRLDKELLKLRGEIARDYYTREDHIRYETLFHAKMDAVGAKVENL
                     ILRSAHAAANQY"
     gene            5092..5406
                     /locus_tag="AC16FV_03305"
     CDS             5092..5406
                     /locus_tag="AC16FV_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849124.1"
                     /translation="MPQPISIEKQRREQLRWLIMTALDAARPLGANEQLILSVVRELI
                     ADLTPRELRRELDYLEERGLIKLAHKDSPTWMAELTRDGIDIVEYTCDCDPGIARPPK
                     YW"
     gene            5409..5954
                     /locus_tag="AC16FV_03310"
     CDS             5409..5954
                     /locus_tag="AC16FV_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HIR2849125.1"
                     /translation="MPTPSKIELLPETIRAELNRRLLANAFSGYEGLAAWLTEHGFDI
                     RKSAVHRYGQKLERKLDAIRASTEAARSIAEAAPDDADLRSAAVMSLVQTEIFEVLVN
                     LQEASDEDVDQVTRVKLLSHAAKNIATLSRASVNQKKWQIEMRDRARDVAEEVKSNAR
                     AAGMSEETAELWRKKILGIAT"
     gene            5951..6511
                     /locus_tag="AC16FV_03315"
     CDS             5951..6511
                     /locus_tag="AC16FV_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849126.1"
                     /translation="MNVKCFWLEPAKRVRVSLRRYEGSKCEHPGHGYHDARVYLEDRP
                     ARFNAEGYIESEDHPKDDSHWPMRCACGYEFSPEDIKQVFVEEIYSRSDTGEEVTLRE
                     APPGAMWDAWWMGNDSRGPDGKALCVKLPDGLDWLVDGPAYSDGHITNPHGWTRQGTP
                     PSITASPSILTKNYHGHLINGELIPC"
     gene            6505..>7918
                     /locus_tag="AC16FV_03320"
     CDS             6505..>7918
                     /locus_tag="AC16FV_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849127.1"
                     /translation="MLETQPAATPPVFLTYQQRWKADLSDVKVAEKSRRVGLSWAEAE
                     DHAGTAAKSKDAGGMDGWYIGYNKDMAQEFIRDVAFWARAMNEACSEVGEEIIKDEDK
                     DILTFVIKFASGFRVTALSSRPSNLRGKQGLITIDEAAFHEDLSGLLKAAMALLMWGG
                     KVRVISTHNGEANAFNELVLDIRAGKVPYSLHRIEFKEAVKQGLYRRICLATKKPWSP
                     EAEAAWVKSMYDYYRDDAPEELDVIPSSGSGVYLTRALIEGIMHADIPVVALTMPSNF
                     AEQPKHIREAEIRDWCERELAPLLKRLNPNWRSFFGEDFARSGDLTVFWPTQETPLLN
                     LVTPFVVELRNIPFEQQKQIVYYIIDRLPRFSAGAFDARGNGQYLAEVAMQKYGATRI
                     AQVMLSTEWYRENMPRFKAAIEDKTINAPRNGNTLADLRAVKMEKGIAKVPDKARVRG
                     ADGQERHGDSAIACALVIYAA"
BASE COUNT         1679 a         2308 c         2438 g         1493 t
ORIGIN      
        1 gacgtcgcgc gcgttctttt tgccgcgcgg gccggcgggc gaattgcgcc cacagctcct
       61 cgcggttgat ggaagtcgct cctgcgcatc cagcgcccgc gacattcgta ctttcctgta
      121 cagcagaaaa aaatcccgcg gtattacccg cggggcaaag acccgtgcca acagggagtg
      181 agtcagcacg ggaaggagac gaaggattca tgatcgccgc ctggacatcg gcgggcagtc
      241 ggtcgagcgg ataaagtcgt gtgatgccac cgcgggtctt ttgctcttgg tgagcccagc
      301 cttcggtggc ggcgcgcatc cgcacggcgc gtggcgtgat cgacaagacg gccgcgatct
      361 gcccggcggt gaagccactc cccttctcta cggtcatttt tagtctccag atttaattct
      421 gttatcgaga ccttcaaaca aatgactcat actgattcct gctttgcggc gcgcggcgtc
      481 gcggcgggcg cgttgccgct cattaattct gtcgcggttg cgctcacgat agagccgatt
      541 cagctctgtg accttgtccc ggttgcgttc acggtaggcg cgctgactgt cacgtttgca
      601 tttacgatag agatctggat tacgctcaag gcgagcgcgc tggctacggt atctgcgttc
      661 gcgttccccc ggctttattc ggtaagccag catgttcagg cagcgggaaa tcagtgcgcg
      721 gaggctcatg cagcctcttt acgggggtct aagccagcgg ccctgatgat tctctcaaca
      781 agcttttgag ccgccggccc ccgccagccg cctaagagtg ccgtccgtgc gttctgcacc
      841 gcgacatggg gcttttgttt atcgcaccat gccgtgaggg tcgttccttt gttgataaaa
      901 ccagcacgga ccgcgttgag cagatcgggt cctgtgtttg ggtgttttgc agattgactc
      961 aagggagtat ccttattaac gcttgatatc aacggttcta agattagaac cgtacagttc
     1021 taatgtcaat accagaacaa aaacaaaata cgccccaaaa tccagccttt ttggcccgcc
     1081 taaaacgcct catcggcgtc gaggcgccat acagctgggc taaacgtatg gggatttcga
     1141 agggggcctt tacccgtatc tacaaagagg gcacggttcc aaccgcaagg ctgctcgaac
     1201 gaatcagaaa aggcaccggt gtatcactgg attggttgct gagaggcgaa ggcgcaatgt
     1261 attcggatcg ggtcgccgaa gaggcgggac cttataaagc agaaaaggga gtgggtccgc
     1321 tgtcgaagga tgaggagttg ttggtacgca agtacaggga actcaatcct tcggataaga
     1381 cccacttcca ggcgattatc gttaaggtgg cttcaacgaa gggagtaggc aaacaggaaa
     1441 ggaagaaata gagttagttg ctggccgtgt catgtgggcc aacaagaatg caggagggga
     1501 acatgtggtt tttgggtttg attatttggt tgcttttgtg tgcactcgcc ggaagtgtcg
     1561 ccgataaaaa aggtcgagcg ggttgggtgt attttgcaat ttcgctattt ttgtcaccgc
     1621 tagtgggtct tctgcttgtt ttgctcttat cgccaatcaa agaaaatatt gagaaacaac
     1681 agatcgagtc tgggcagaat cgtaaatgtc ccttctgcgc cgagatcatc aaggccgagg
     1741 ctaaaacctg taagcattgc gggaaggatt caccgccttc gttgccagga ccgaagttcg
     1801 accctgatac gggagcgatg atttgacatg actcctgaca aacgagaacc cgttgtaatt
     1861 tataacccca agcaagacca gttcctcacg cgcaaccgcg gatgcctgga gagtttgttc
     1921 tggttatgcc tcattggaat cgctctcttt tggctcttcg gttgtgcgcc ggccacggtt
     1981 caaggactgc gagaaaacca cgccggaaag atcacgttcg atgtggacga aaattaccag
     2041 tcggtttatc gcaaggtgct cacgccggcg cgcgactgtt ggcagagcgg attgatcacc
     2101 gcccagatgg ttgttcaggg cgatttgtat actgacacct ggcgcgggaa tgtcacggta
     2161 gcgttgcaca gcggcgccgg cgtcgatacc tatttcaccg tcgatgtgga ggcgctgtcg
     2221 gaggcgaaga cccgggttac cgtttatcac gcgttccgaa atcaggaaag agccgcgcaa
     2281 gcggttgagc tgtgggttaa gcagaactcg aagctgtgtc gtgtaccggg accgcaagaa
     2341 gaattataat cccagctccc gtttcaaacc cttctttaaa acccgctaat attccgtccc
     2401 cggcgcgatc tctatgatcg cgccatgcgt gatcgtttcg atatttgtct ggacttcgtc
     2461 ctgccggagg agatcggccc gttcaaggcc acggcgggtt atatctccgt cgcgcgtgcg
     2521 gcgcaaatcg gcgacactgg cagcgagacc attgccggcg tttcgcgcgt gcacaatccc
     2581 gactggcccg gctgggcgat cgtcgatagc cgcaagaccg aaccggactt ccccaagaat
     2641 ctcaacctcg atctgccacg caacgccgag ctgaagcgcc tggtcggcga agtctatcgc
     2701 gtgaaatact ggcagggctt ccgctgcggt gagatgccgg cggggctcga tctcgcgctc
     2761 ttcgacgctg cggtgcaaca tctgccgaag ccggcggccg tgctgttcca gcaggccgtg
     2821 caggccaaac ccgatggtga catcggcgac ctgacggtga aggccgcggg ctacgccaac
     2881 gtgccccgtg ccttgttcac ctatttcgtc cggcgcaccg agttctacat ggacatcatc
     2941 ctcgccgaca gccgccaagc gaagaatcgt tccggctggt tttcccgcgt gttccggctg
     3001 caagcttaca tcctcaccac cacaccgatc gtatggggag gcaacccatg aaccacgaaa
     3061 tgctcgccca ggaattgacg ctcttcgcgc ccggcctggc cacggcgctg gccgaagaca
     3121 cgaccgatgc gctggccacc aaggcggtcg aaaccgcgcg ttacgtcacc ggcgtctaca
     3181 gtccggacat ggcggtgacg gcgatcaaga acgatccggc ggcgcaggag cgcttccgca
     3241 aggcgatggc gtacctgctc acggtcgaga ctcgcgcccg tgcccccgag gcgccggccc
     3301 cggccgccgt gggcgaaaag gatttgctcc aatcgaaagt cacctggggc gtgctggtgc
     3361 cgattatcgc catgcttacg agccgatacg gactcgatct cggcgatcag cagggatggg
     3421 tggatgccat ctccggcctg gtcggtgcgt tgctgatttt ctggggccgg gtcgcggcca
     3481 tcaaacgagt tacctcaatc gcaggagtca aaatcgatgc caaaccgtaa acaggctttt
     3541 ctctcgttct tcgttgcact gctcgcgctg gcgctcgccg cctgcgccgc ggtgccgcag
     3601 ccgcgctcgt tcaccgacca ggtcacgatc gcgcgcgccc tgctcgcggg cgtggacaaa
     3661 tccatcggcg atcttacaga ggccggcgtg ctgcattcca gcaaagcgca gcagctgctc
     3721 gacaaggcca agcaggcgcg ccgtgacatc gagcaggccg agacgctgta tcgcaccggc
     3781 aagcaggtgg acgcgcagaa cctgctggcg accacgcaaa ccgtgctgct cacgctccgt
     3841 gagcagctca tgaaaatgga gaagcagcca tgaccgaagc agggctcatt aaagttctcg
     3901 acctcatgat cgtcggctcg aacatcggca tgaacatcaa cgcgcagctg gaaaagttcc
     3961 ggctgctggt gagccagagc aagaccgacg aggagatcgc cgagatcttc aagcaggacc
     4021 tggccgctgc cgaggtcgag gcgcaggcga agatcgatgc cgcgaaacgc ctcgggcgct
     4081 gacagtatga aggacgtaag ccagcgcctg gtcgaagcgg agcgggagcg cgaccggctg
     4141 cgcttccggg tgcgctatgg cgtctggcca ccggtgggat tgccgccgag cgaatggctt
     4201 aagcatgacc agaaatggcc gaaggatatg aatgcgctgg agcggctgca ggtcgagaac
     4261 gaagtgcttc gggaagaagt gatgatgctg atgcgaggca atcaatcaga tgaaattatt
     4321 ccggcggtaa ttgtcagcgc acttgacttt ggcgcccgct tcagcgcgag cctctacagt
     4381 ctgccgccgg gtcatcaagt gcacctggcg cgcgatctgt cgacggcggt gcaagcggcg
     4441 ctggacgaac ttgagcggct acggccggga tcgctgggca aaagtgcatc gcatggctga
     4501 tgccgccgac tatgcccagg cgatcgcgga agaagagcgc aaggatgcgc tggccgcgcg
     4561 tgcggcgcga acgttccacg aggaacctcg cgtcgtgaat gggcagcggt tgtgccgcga
     4621 ctgtgacgag ccgctctcga agaagcggct caaatttgta cccgaggcgg tgcgttgcgt
     4681 ggattgccag aatcttctcg aacaaagaca acggagaaag aaattatcat gacaagacga
     4741 gcgaagcgca gcgaagcagg gctacgttaa tgcagattgt gatctcgtgg gaaatggtgg
     4801 gcacggccgt cacgatcctg ctggcgctgt tcggcctgct ggtgggcatc atccgctggc
     4861 tgctcgatcg ttatgtggat gcgatcaaat cgcagatcga aatcatggcc aagcaaatca
     4921 ccgcgcaggc cggtgacatc acgcggctcg acaaggagct gctgaaactg cgcggcgaga
     4981 tcgcccgcga ttactacacc cgcgaggacc acatccgtta cgaaacgctg ttccatgcca
     5041 agatggatgc cgttggagcg aaagtcgaaa acctgatcct aaggagcgcg catgccgcag
     5101 ccaatcagta ttgagaaaca acgacgcgaa caattgcgct ggctcatcat gacggcgctg
     5161 gacgccgcgc gcccgctcgg cgcgaacgag cagctgatcc tgtccgtggt gcgcgagctg
     5221 atcgcggacc tcaccccgcg cgagctgcgc cgcgaactcg attacctcga ggaacgcggc
     5281 ctgatcaagc tggcgcataa ggacagcccg acctggatgg ccgagctgac gcgcgatggc
     5341 attgatatcg tcgagtacac ctgcgactgc gatcccggca tcgcgcgtcc gccgaagtat
     5401 tggtaaccat gcccacgccg tcaaaaatcg aactcctgcc ggagacgatc cgcgccgagt
     5461 tgaaccgtcg gctcctcgcc aacgcttttt ccggttacga ggggctggcc gcatggctca
     5521 cggagcatgg ttttgatatc cgtaaatccg ccgtccatcg ctatggccag aaactggagc
     5581 gcaagctcga cgcgatccgc gcctcgaccg aggccgcgcg ctcgatcgcc gaggccgcgc
     5641 cggacgatgc ggacctgcgc agcgcggcgg tgatgagcct ggtgcagacc gagatcttcg
     5701 aggtgctggt gaacctgcag gaagccagcg acgaagacgt ggaccaggtc acgcgcgtga
     5761 agctcttgtc gcacgcggcc aagaacatcg ccacgctctc gcgcgccagc gtgaaccaga
     5821 agaaatggca gatcgaaatg cgcgatcgcg cgcgcgacgt ggccgaggaa gtgaagagca
     5881 acgcccgcgc cgccggcatg agcgaagaga ccgccgagct gtggcgcaag aagattctgg
     5941 ggattgcgac atgaacgtca aatgcttctg gcttgaacca gccaagcgtg tgcgcgtgtc
     6001 gctgcgccgt tacgaaggct cgaaatgcga acacccgggg cacggctatc atgacgcccg
     6061 ggtttatcta gaagatcggc ctgcgcggtt taatgccgaa ggatatatcg agagcgagga
     6121 tcatccgaag gatgactcgc actggccgat gcgctgcgcc tgcggatacg aattttctcc
     6181 cgaagacata aaacaggtgt tcgtagagga aatctattcg cgctcggata ccggcgagga
     6241 ggtcactctg cgagaagcgc cccctggcgc catgtgggat gcgtggtgga tgggcaatga
     6301 cagccgagga cccgacggca aagcgttatg cgtgaaactg cccgacggtc ttgattggct
     6361 tgttgatggc cccgcttata gcgatggcca tatcacgaat ccgcatggct ggacgcgcca
     6421 aggcacaccg ccatcgatca ctgcatcgcc atcgattctg acgaaaaatt atcatggcca
     6481 tctaattaat ggtgagttga ttccatgcta gagacgcaac ccgccgccac accgcccgtc
     6541 ttcctgactt accagcagcg ctggaaggcc gatctgtcag atgtcaaggt cgccgagaaa
     6601 tcgcgccgcg tgggtctctc ctgggccgag gccgaggacc acgccgggac cgcggcgaaa
     6661 tccaaggatg ccggcggcat ggatggctgg tacatcggat acaacaagga catggcgcag
     6721 gagttcatcc gcgatgtcgc cttctgggcg cgcgcgatga acgaagcatg cagtgaggtc
     6781 ggcgaggaga ttattaagga cgaggacaag gacatcctca ccttcgtcat taagttcgcc
     6841 tcgggcttcc gcgtcaccgc gctctcctcg cgcccgtcga acctgcgcgg caagcagggc
     6901 ctcatcacca tcgacgaagc cgcgttccac gaggatctgt ccggcctgct caaggccgcg
     6961 atggcgctgc tgatgtgggg cggcaaggtg cgcgtgatct ccacgcacaa tggcgaagcg
     7021 aacgccttca acgagctggt gctcgacatc cgcgccggca aggtgccata cagcctgcac
     7081 cgcatcgagt ttaaagaggc ggttaaacag ggtttatacc ggcgcatttg tctcgccacg
     7141 aaaaagccct ggagcccgga ggccgaggcc gcgtgggtga agtcgatgta cgactactat
     7201 cgcgacgatg cgccggaaga gctggacgta atcccgtcga gcggcagcgg tgtgtatctc
     7261 acgcgcgcgc tcatcgaagg catcatgcac gccgatatcc cggtggtggc gctcacgatg
     7321 ccatcaaact tcgccgaaca gccgaagcat atccgcgaag ccgaaatccg cgactggtgc
     7381 gagcgcgagc tggcgccgct gctcaagcgg ctcaatccca actggcgcag cttcttcggc
     7441 gaggacttcg cgcgcagcgg tgacctcacc gtattttggc cgacgcaaga gacgcccttg
     7501 ctcaacctgg tcacgccctt cgtcgtggag ctgcgcaaca ttcccttcga gcagcagaag
     7561 cagatcgtct actacatcat cgaccggctc ccgcgcttct ccgccggcgc cttcgacgcg
     7621 cgcggcaacg gccagtatct ggccgaggtc gcgatgcaaa aatacggcgc cacgcgcatc
     7681 gcccaggtga tgctctccac cgagtggtat cgcgaaaaca tgccgcgctt caaggccgcg
     7741 atcgaggaca agaccatcaa cgcgccgagg aacgggaaca cgctcgccga tctgcgcgcg
     7801 gtgaaaatgg agaagggcat cgccaaggtg ccggacaagg cgcgcgtgcg cggcgccgac
     7861 ggccaggagc gccacggcga cagcgcgatc gcctgcgcgc tcgtgatcta cgccgcca
//
LOCUS       DBNGZE010000130         6324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_157322, whole genome shotgun sequence.
ACCESSION   DBNGZE010000130 DBNGZE010000000
VERSION     DBNGZE010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_157322"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            613..2397
                     /locus_tag="AC16FV_03325"
     CDS             613..2397
                     /locus_tag="AC16FV_03325"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR2849128.1"
                     /translation="MLLPNKKQRAISLLREKKFPEAKQFLEEIVLSDRTDVDAWIYLS
                     MIYAQTGPVGDFERCCRGAIAANPGLPNAHFNLGVALGSQGKVNEAIQSYRQAIRLKP
                     DHVLALFNLGHSLQTTFCLEEAFSCYSHVLSIKLTPADPVPANFHATVHHYLANVLKA
                     QGRMKDALVHYRQALDLNPGMTGAHSDMLLALNYDPPDATSVFDEHVKWAGRHGYSGP
                     VSFQHRHSQKPDRPLRVGYVSPDFYKHAVTFFFEPLLSHHNRSRIEPVCYSLTQKSDE
                     VTARLRSAAAQWRNVFAMNDEQLANQIYADQIDILVDLTGHMGENRLPVFSRKPAPIQ
                     VSWLGYPNTTGLTTIDYRLTDAFADPPGLTDRFNTEQLLRLPRGFLCYQPPVTAPAIG
                     PLPSMGNRIITFGSFNNLSKITSGTVHLWSEILRAISGSRLILKNASFTDIPTRDPYY
                     REFEKHGIARARLDFRGMHRELTDHQSVYNEIDIALDPFPYNGATTTCEALWMGVPVI
                     TLAGDRHAGRVGASILTQVGMTELIAGNPDDYVRIAVELANNPGRLAEWRNTLRQRMA
                     ASPLCDAKGFALNMEEAYRTMWKKWCVS"
     gene            2399..4291
                     /locus_tag="AC16FV_03330"
     CDS             2399..4291
                     /locus_tag="AC16FV_03330"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849129.1"
                     /translation="MTTSVDSLCMLGEQELSSGNLKNAAAAFQRAIELQPTHAPAWSG
                     LGRCFQSAGQTEQAMRCFDNALVHDPDHLPSRFGRAVILHDLGNLPEARRAYEELLKV
                     APGQPVFHNNLGTILDAMQDLQGAERCYQSAIRLEARYATAWANLAGLKTRQNRIAEA
                     REYYTRATALDPVDQVKAMLRDFLCPAFYADRNAIVTYRAEYLDVLRRWQSTPPRSSI
                     EALWQAGICPPFNTLYHGLDERPLREAFSAMLAPWFAASSANRDAAQARTTHPGQMRV
                     GLLVSPGHEGIFLRSMQGVIERLDARFRIFIVCGASGKPALERTLRRDELGFVILPGT
                     LEGMVAAVREARLDILYHWEIGTDATNYMLPHYRPAPVQCTSWGIQVTSGIAAVDYYL
                     SSRFAEPENAQAHYTERLLLATAPLTYQFRVHAGRLAIETRRRYGWDPADHLYACIHQ
                     PGKFHPDFDHVLGGILRRDRKGKIVLTRDAGTYVSLQLQQRFRHTLADVADRIVFLPY
                     LGSDEYLNVLAACDVTLNPLHYGGVNTTFDALSLGVPPVAMDGERQIARYAAGCLRHI
                     GMHELVASDLSSYAEIAARLGTDDAWRSEVNARLSERSGALFENIESVREHERLFDLM
                     VQETRS"
     gene            4351..5460
                     /locus_tag="AC16FV_03335"
     CDS             4351..5460
                     /locus_tag="AC16FV_03335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2849130.1"
                     /translation="MKVAFVDNAGLAYTPETPYQRPLGGIQSAVCYLAAEMNKKGWQP
                     RLFNAVEEATEGRGVPAYPLNISSVKQLAECDLVISVGGAELVHPVRNVVGFGKPVFM
                     WTGHAHDQPAVQGLSDPAIHQAYNGFIFVSEWQRQQFHRQFAIAANRSIVLRYGMGSC
                     FAGDKSAAELLGGKADPPVIAYTSTPFRGLDLLLETFPAMKREIPELRLNVYSGMQVY
                     GVDRAADPYNPLYQTARAIDGVNYIGSIPQPELARQMAAVTALVYPNTFAETSCISVM
                     EAMISGCGVVTTALGALPETTAGFATLIQPDADRIAYKERFVAAAIGLLRRFQSPDAQ
                     LIDQLQSQIDFCRKHYYWPHIADQWIAAFDRGARG"
     gene            5463..>6324
                     /locus_tag="AC16FV_03340"
     CDS             5463..>6324
                     /locus_tag="AC16FV_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849131.1"
                     /translation="MATDELTIRNELEGFIRASGADVSAQMKQVRSLMDLYFMHGQWA
                     CTNDLPEKAIRSFKACLKIVPDAPIIHCALGRIHLTQGELIKAERHARRALELQPNDL
                     KNILTSRLIIAKLGRDVVAEDRLALPIRSRIAAAARSYGLAAPVWPWGPRVEAGAWCA
                     EQLRKAAAEFRAHRFTVLRGVLPAGWIELLKAEQAALLAGGAMSLEPDMHRYATVDAP
                     MAAVANYEVAGLVERVLGKGVIPTYTYAIHYLPNGHIRPHTDRPQNELSMSLSLAVTP
                     AGKDVSVLRAG"
BASE COUNT         1294 a         1828 c         1833 g         1369 t
ORIGIN      
        1 actgacgtta acagcttatc tttaagctaa ttatttttca gttcttgcaa tggacctaat
       61 gtattcctac cccacccagt gcgaattttc atgtatgttg cacctctgaa ataataattg
      121 tcaaggcgct tataccccat tggctaaacg aatatccgtc tggttgcgta tattaagtgt
      181 tatcaggcgt ttcatacatt tagaggctcc gccacgccga taatgactgg cgtggcgccg
      241 tggggaattg actttgaagt caatgtaagg ttggaaaagc cggtgatgag agggtagagc
      301 cggatcacag atttagaccc atgttcatat tttgtgcccg acttgctaca aattctttac
      361 gggattgcgc gcggttcgaa tgcgtctacc agccgatcta gacaggtcct gtgtctggac
      421 ggttgtacgt gaatggattg tcttcagttt aattgcctgt cgctcagcat ttgcataatt
      481 tcactgacca ttgagtgtat taaatcaaaa tattcttata ttcttcactt acgttttaca
      541 gatttgaatt attgaagcag caccaagcct tttggtttgg gagctataca gcgagtaatt
      601 gtcattgccc caatgcttct gcccaacaag aaacaacgcg caatatcgct actcagggaa
      661 aaaaaatttc ccgaagcgaa acaatttctg gaagagatcg ttctgagcga tcggacggat
      721 gtcgatgcgt ggatatatct ctcaatgatt tatgcccaga ctgggcctgt cggtgacttc
      781 gagcgatgct gtcgcggcgc aatcgcggcc aatcccggtc tgcccaatgc gcatttcaat
      841 ctgggcgtcg ctctgggctc gcagggaaaa gtaaacgagg ccattcagag ctatcgacag
      901 gcaataagac tcaaacccga ccacgttctg gcgcttttta atcttggaca ttcattgcag
      961 accacttttt gcctggaaga agccttctcc tgctattcac acgtactgtc catcaaatta
     1021 acgcccgccg accctgtgcc ggcgaatttt cacgccacgg ttcaccacta cctggcgaac
     1081 gttctcaagg cgcagggaag aatgaaagat gcgcttgttc attatcggca ggcccttgac
     1141 ctgaatcccg gcatgaccgg cgcgcacagt gacatgctgc tggcgctgaa ttatgacccg
     1201 ccggatgcca cctccgtctt tgatgagcac gtgaagtggg ccgggcgaca cggctattca
     1261 ggcccggttt cctttcagca ccgccattca cagaaacccg atcggccgct gcgagtcggt
     1321 tacgtgtcac cggattttta caaacatgca gtgacgtttt ttttcgaacc gttgctttct
     1381 catcacaacc gttcaaggat agaacccgtg tgttattctc tgacgcaaaa gtcggacgag
     1441 gtgaccgcgc gcctgcgctc ggctgcggcg caatggcgca atgtctttgc catgaacgac
     1501 gagcaattgg ccaaccagat ttacgccgac cagattgata ttctggtcga tctcaccggg
     1561 cacatggggg aaaacaggct gccggtgttc tcccgcaagc cggcgccgat ccaggtatcc
     1621 tggcttgggt atccgaatac gacggggttg acgaccattg attaccgact gaccgacgcc
     1681 ttcgcggatc cgccggggtt gaccgatcgt ttcaacacgg agcaactgct caggttaccg
     1741 cggggtttcc tctgttatca gccaccggta acggcgccgg cgattgggcc gttgccttcc
     1801 atggggaaca gaatcatcac ctttggttcc ttcaacaatc tgtcgaagat tacgtccggc
     1861 acggttcact tgtggagcga gatactgcgc gccatatccg gttcacgcct gatattgaaa
     1921 aatgcgtcgt tcacggatat ccccacgcgg gacccttatt atcgggagtt cgaaaaacac
     1981 ggtatcgcgc gcgcacggct cgattttcgc ggcatgcatc gggagttgac cgatcatcag
     2041 tccgtgtata acgagatcga catagctctt gaccccttcc cctacaatgg tgccaccacg
     2101 acctgcgaag cattgtggat gggtgtgccg gtcatcacgc tcgccggcga ccgccacgcc
     2161 ggccgtgtcg gagccagtat cctgacacaa gtggggatga cagagctgat cgccgggaat
     2221 cccgatgact acgtccggat cgccgtggaa ctcgcaaata atcccggccg cttggcggaa
     2281 tggcgcaata ccttgcgtca gcgcatggcg gcttcgcccc tgtgcgacgc gaagggcttc
     2341 gccctgaaca tggaagaggc ctaccgtacc atgtggaaaa aatggtgcgt gtcgtgaaat
     2401 gacaacgtcc gtagacagtt tgtgtatgct gggcgagcag gaactttcga gtgggaatct
     2461 gaaaaacgcc gccgcggcct ttcagcgcgc gatcgaattg caaccaaccc acgcaccggc
     2521 atggagcgga ctgggacgat gtttccagtc tgccggccag acggagcagg caatgcgttg
     2581 ctttgacaac gcattggtcc atgacccgga ccatctgccg tcgcgattcg gccgcgccgt
     2641 gattcttcat gacctcggca atcttccgga ggcacgacgg gcctacgagg agttgctgaa
     2701 agtcgcgccc ggacagcccg tgttccacaa caatctcggg accatcctcg acgcgatgca
     2761 ggacctccag ggcgcggagc gctgctacca gtccgccata cgcctcgaag cccgatatgc
     2821 gacggcgtgg gcgaaccttg ccggactgaa gacccgacag aaccggattg cggaggcaag
     2881 ggaatattac acgcgcgcca ccgcgctcga tccggtggac caggtaaagg ccatgcttcg
     2941 cgatttcctg tgccccgcgt tctatgcgga ccgcaacgcg atcgtcacat accgtgctga
     3001 atatctggat gtcctgcgcc gatggcagtc aacgccgcca cgcagcagca tcgaggcgct
     3061 gtggcaggcg ggaatctgtc ctccattcaa cactctctat cacggcctcg acgagcggcc
     3121 gttgcgcgag gccttttcgg ctatgcttgc gccctggttt gccgccagtt ccgcgaatcg
     3181 ggacgcagcg caggcgcgca cgacgcaccc gggccagatg cgtgtcggcc tgctggtctc
     3241 tcccgggcat gaaggcattt tcctgcgatc catgcagggc gtcatcgagc ggctcgatgc
     3301 gcgattcagg atattcatcg tttgcggcgc ctcaggaaag ccggcactgg agcgcactct
     3361 caggcgcgac gaattgggtt tcgtcatcct tccgggcacg ctcgaaggca tggtggccgc
     3421 cgtcagggaa gcccggctcg acattcttta tcattgggaa atcggcaccg acgccaccaa
     3481 ttacatgctg ccgcattacc gccccgcgcc ggttcaatgc acatcgtggg gaattcaggt
     3541 aacgtcgggc attgcggcgg tggattatta tctgtcgagc cggttcgcgg aaccggaaaa
     3601 cgcgcaggcg cactatacgg aacgactgct gttggcgacg gcaccgctga cctatcaatt
     3661 tcgcgtgcat gccgggcggc tcgcgatcga aacgcggcgt cgttacgggt gggacccggc
     3721 cgatcacctt tatgcctgca ttcatcagcc cggcaagttc caccccgatt tcgaccacgt
     3781 gctgggcggc atcctgcgcc gcgaccggaa aggaaaaatc gtgctgaccc gtgatgccgg
     3841 aacatatgtc tccctgcaac tgcaacagcg attccggcac acgctggcgg acgtggcgga
     3901 tcgcatcgtg ttccttccct atctcggtag cgacgaatat ctgaacgttc tggccgcatg
     3961 cgacgtcact ctcaacccgc tccactatgg aggcgtcaat acgacattcg acgcgctctc
     4021 gctcggcgtg cctcccgtgg cgatggatgg cgagcgccag atcgcgcgct acgcggcggg
     4081 ttgccttcgc catatcggca tgcatgaatt ggttgcgagc gatctctcct cgtacgcgga
     4141 aattgccgcc aggctcggta cggatgacgc gtggcgctcc gaggtaaacg cccggctcag
     4201 cgagcgaagc ggcgcattgt tcgagaacat cgaatccgtg cgcgaacacg agcggctctt
     4261 tgatctaatg gttcaggaaa cccggtcgtg atgaacattc gccgtgcgcg cggtggctcg
     4321 ttattcggat caatctgaca atccattacc gtgaaagtcg cctttgttga caacgccggt
     4381 ttggcttata cgccggaaac gccgtaccag cgtcccctcg gcggcattca gtcggcggta
     4441 tgctatctcg ccgcggaaat gaacaagaag ggctggcagc caaggctttt caacgcggtg
     4501 gaagaggcca ccgaaggccg gggcgttcct gcctacccgt tgaacatctc ttccgtaaag
     4561 caactggctg aatgcgattt ggttatttcg gttggcggtg ccgagctggt gcatccggtt
     4621 cggaatgtcg tgggtttcgg caaacctgtt tttatgtgga ctggccatgc ccatgatcaa
     4681 ccggcggttc aggggttgtc cgatccggcg atccaccagg cgtacaacgg attcattttc
     4741 gtcagcgaat ggcagcgaca acagtttcac cggcaatttg ccatcgctgc caaccgctcc
     4801 atcgtcctgc gatacggtat gggctcctgt ttcgccggtg acaaatccgc cgcggagctg
     4861 ctcggcggca aggcggaccc gccagtgatc gcatacacca gcacgccgtt ccgcggcttg
     4921 gatctgctgc tcgagacatt tccggcgatg aaaagggaga tcccggaatt gcggctcaac
     4981 gtctactccg gcatgcaggt ttacggcgta gaccgggcgg ctgatccgta taatccactg
     5041 taccaaaccg cccgcgcgat tgacggcgtg aactatatcg gctcgattcc gcaacccgag
     5101 ctggcgcgac aaatggcagc ggtgacggca ttggtgtatc cgaacacttt tgcggaaacc
     5161 agctgtattt ccgtgatgga ggccatgatc agcggctgcg gggtggtcac caccgcgctt
     5221 ggcgcgctgc cggagacgac ggcgggcttc gcgacgctga tccagccgga tgccgaccgc
     5281 attgcctaca aggaacgctt tgtcgcagcc gcgatcgggc tcctgcggcg ttttcagagc
     5341 cccgacgcgc agttgattga tcagctacaa tcgcaaattg atttttgccg aaagcattat
     5401 tactggccgc atattgccga tcagtggatc gcggcattcg atcgtggcgc gcggggataa
     5461 caatggcaac agatgagctg acgatcagga acgagctcga aggcttcatc cgtgcatccg
     5521 gcgccgacgt gagcgcgcag atgaaacagg tgcgtagcct gatggatctt tatttcatgc
     5581 acgggcaatg ggcgtgcaca aacgatctgc cggagaaggc catccgctcc ttcaaggcct
     5641 gtcttaaaat cgtccctgac gcgccgatca tccattgcgc cctcggtcgt attcatttga
     5701 cgcagggtga gcttatcaag gcggagcggc acgcgcggcg tgcgcttgag ttgcagccca
     5761 atgacctcaa gaatatcctg acgagccggc tgatcatcgc gaaactcggc cgcgatgtcg
     5821 tggccgagga ccggttggcg ctgccgattc gttcccggat cgcggcggcc gcgcgatcgt
     5881 acggacttgc cgcgccggtc tggccctggg gtcccagggt ggaggcgggc gcctggtgtg
     5941 cagaacagtt gcgcaaggcc gcggcggagt tccgggcaca caggttcacg gttttgcgcg
     6001 gcgtcttgcc ggcgggttgg attgagctgc tgaaggcgga acaggcggcg cttctcgccg
     6061 gcggtgcaat gagccttgaa cccgacatgc accgatacgc gacggtggac gcgcccatgg
     6121 ccgcggtcgc caactacgag gttgccggtc tggttgagcg tgtcctgggg aagggcgtga
     6181 ttcccaccta tacgtacgcc attcattatc tgccgaacgg tcacatacgg ccgcacaccg
     6241 accggccgca gaacgagcta tccatgtcgc ttagtctcgc cgtgacgccg gccggcaagg
     6301 atgtatcggt actacgtgca ggac
//
LOCUS       DBNGZE010000131         1979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1268464, whole genome shotgun sequence.
ACCESSION   DBNGZE010000131 DBNGZE010000000
VERSION     DBNGZE010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1268464"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16FV_03345"
     CDS             complement(<1..619)
                     /locus_tag="AC16FV_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2849132.1"
                     /translation="MPVEHPKALIMSLPPKPGIYRMLDGKGEVLYVGKAHNLRKRVQS
                     YFRASGLAARVMLLMQQAQNIEITVTHTETEALLLENNLIKTLHPRFNILLRDDKSYP
                     WIFVSEGQAYPRIGFHRGAKREKGRYFGPYPSSYGVRETLNLLQKIFRVRQCQDSFFR
                     NRSRPCLQYQIKRCSGPCVGLIEPERYAADVQQAVMFMEGKSQSLI"
     gene            complement(941..1573)
                     /locus_tag="AC16FV_03350"
     CDS             complement(941..1573)
                     /locus_tag="AC16FV_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR2849133.1"
                     /translation="MPEFDPHCVACPRLASHLSNVRDMYPTYYARPVPPFGDPKARLI
                     VIGLAPGMHGANRSGRPFTGDHAGILLYETLYKHGFSDRRESKSARDGLRLKNCRITN
                     AVKCLPPANKPLPAEIATCNLYLQTELTKLAPGSVLLALGTVAHEAALKAFKLKKKDY
                     KFAHAAHHKLPNGLHLLDSYHCSRYNTQTKRLTAEMFDKILHKAKGLLAG"
     gene            complement(1819..>1979)
                     /locus_tag="AC16FV_03355"
     CDS             complement(1819..>1979)
                     /locus_tag="AC16FV_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HIR2849134.1"
                     /translation="HTKMMLKDSTLDNYLFGVKEAELSPQDNKVVTQRLKHEMAEIFY
                     GTNMVRVN"
BASE COUNT          414 a          516 c          617 g          432 t
ORIGIN      
        1 ggatcaggct ctggctcttg ccctccatga acatcaccgc ctgctgcacg tcggcggcgt
       61 agcgctcggg ctcgatcagg ccgacacagg ggccggagca gcgcttgatc tggtattgca
      121 ggcaggggcg cgagcggttg cggaagaagc tgtcctggca ctggcgcacg cggaagattt
      181 tctgcagcag gttgagcgtc tcgcgcacgc cgtaggacga cggatacggg ccgaagtaac
      241 gccccttctc gcgcttggcg ccgcgatgga agccgatgcg cgggtatgcc tgcccctccg
      301 agacgaaaat ccaggggtag ctcttgtcgt cgcgcagcag aatgttgaag cgcgggtgca
      361 gcgtcttgat aaggttgttt tcaagcagca gcgcttcggt ttcggtgtgg gtgacagtga
      421 tctcgatgtt ctgcgcctgc tgcatcagca acatcacgcg tgccgccaga ccgctggcac
      481 gaaaataact ctgcacgcgc ttgcgcaggt tatgcgcctt gccgacgtac agcacctcgc
      541 ccttcccgtc cagcatgcga tagatgccgg gcttgggcgg cagcgacatg ataagggctt
      601 tggggtgttc gacgggcata aattatttaa accgccaggg cgccaagggc gccaggaaaa
      661 acatactggg gttaaaacca agttcacagc aaagacgcga aataaagttt tgaaccgcca
      721 agacgccaag aacgcaaaga aagacatatt ggggttaaaa ccgagttcat tataagttgg
      781 gtttgggtat tgtcagtccc gcggaggcgg gaatccagtg cgatttatgt catcgcgcgc
      841 aacgcgcacg ataccattca aagcctgggt ccccgctttg cgtccttagc tccgtaatca
      901 gcacttccct gtgctacttc tcgcggggac gacagaatca ttagcccgcc aacaaaccct
      961 tcgccttgtg gagaatcttg tcgaacatct ccgccgtcag gcgcttggtc tgggtgttgt
     1021 agcggctgca atgataggag tcgagcagat gcaggccgtt gggcagcttg tgatgggcgg
     1081 cgtgggcgaa tttgtagtct ttctttttta gcttgaaggc tttcaacgcg gcctcgtgcg
     1141 ccaccgtgcc gagcgcgagc agcacgctgc ccggcgcgag ttttgtgagt tcggtctgga
     1201 gataaagatt acaggtggcg atttccgccg gcaacggctt gttggccggc ggcaggcatt
     1261 tgacggcgtt ggtgatgcgg cagtttttga gtcggagtcc gtcgcgcgcg gatttggatt
     1321 cgcgccggtc ggagaagcca tgcttgtaaa gcgtttcata caaaaggatg ccggcgtgat
     1381 cgccggtgaa cggccgcccg ctgcgattgg cgccgtgcat gccgggcgcg agcccgatga
     1441 cgatcaggcg cgccttgggg tcgccgaacg gcggcaccgg ccgcgcgtaa tacgtcgggt
     1501 acatgtcgcg gacattggac aaatggctcg ccagccgcgg acaggcgacg caatggggat
     1561 cgaattcggg cattggatgt cggtaaacag cgataccgac atcctaacgg acttcgggcg
     1621 ggaacgacaa atttttatga ttatttgccg cagagacgca gaggatgcgg agaaaagctt
     1681 ttttgaagtt aaaacaagaa gctaaccaca aagtgaattt gcatcgggaa aaactgggtg
     1741 cgcaacaacg tggaccgaaa atttttttca ggtttttctt tgcgtcctct gcgtctctgc
     1801 ggcagaaatt aataaatctc aattcacccg caccatgttg gtgccgtaga aaatctccgc
     1861 catttcgtgt ttcaggcgct gggtgacgac cttgttgtcc tgcggggaga gctcggcctc
     1921 tttcacgccg aagagatagt tatcgagcgt ggagtccttc agcatcatct tggtgtgga
//
LOCUS       DBNGZE010000132         2236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1685900, whole genome shotgun sequence.
ACCESSION   DBNGZE010000132 DBNGZE010000000
VERSION     DBNGZE010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1685900"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="era"
                     /locus_tag="AC16FV_03360"
     CDS             complement(<1..507)
                     /gene="era"
                     /locus_tag="AC16FV_03360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR2849135.1"
                     /translation="MPDATTRSGLVTLAGRPNVGKSTLLNRLVGQKISITSSRAQTTR
                     HRLLGIKTTEQAQIVYVDTPGLHTDNKGVMNRHMNRVADASLHGVDCIVLLIAASGWT
                     KADEYPLQLVARQGVPVILAINKLDQLEDRAKLLPLMELSSRKAEFAEIVPVSARTGA
                     NTDDLENAI"
     gene            complement(507..1175)
                     /gene="rnc"
                     /locus_tag="AC16FV_03365"
     CDS             complement(507..1175)
                     /gene="rnc"
                     /locus_tag="AC16FV_03365"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR2849136.1"
                     /translation="MPAELVRRLEYTFRQPELLERALTHRSKSAANYERLEFLGDSVL
                     GFAISSELYNRYPNLYEGELTRLRASLVKKETLAALARNLDLGNYLKLGEGELKSGGY
                     DRDSILADALEAIFGAICKDSSVTEAMRVILHLYHDKLVGLDPHSIPKDPKTQLQEYL
                     QKLSLPTPTYLVRDVTGEPHNQSFVVECHVTVLDRPVRGEGNSRRHAEQEAAAQALEL
                     LHRR"
     gene            complement(1177..1557)
                     /locus_tag="AC16FV_03370"
     CDS             complement(1177..1557)
                     /locus_tag="AC16FV_03370"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR2849137.1"
                     /translation="MQNPKRQSGMTMWSLLFVLGTLAFFLFLLFKLIPPYLGDMKVKS
                     ALESLGRQPDAGSMSVPEISEAIRKRLEIDSADDFDLSKSLTITAKGRTKLIRINYES
                     VTPILYNVSVLLTFDHSIEVRGTE"
     gene            complement(1594..>2236)
                     /gene="lepB"
                     /locus_tag="AC16FV_03375"
     CDS             complement(1594..>2236)
                     /gene="lepB"
                     /locus_tag="AC16FV_03375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR2849138.1"
                     /translation="VILIVFFIRSFLVEPFRIPSGSMLPSLFIGDFNQVNKFSYGVRL
                     PVANNKILETGQPKRGEVAVFRFPGNPSINYIKRIVGVPGDHILYKDKKLYINGTLME
                     QADGRPYYFASGGDAQGEALRLMESLDGVKHDILTTNRMDPPLPEIVVPAGKYFVMGD
                     NRDHSNDSRYWGFVPDQNLVGRAFLIWFSWDVTGQEKWFWQRIVWDRVGNSIY"
BASE COUNT          474 a          646 c          621 g          495 t
ORIGIN      
        1 aatggcgttt tccagatcgt ccgtattggc gccggtgcgc gccgagaccg gcacgatctc
       61 ggcgaattca gctttccgac tcgatagctc catgagcggc agcagcttgg cgcgatcctc
      121 gagctgatcc agcttgttga tagccagaat cacgggaacg ccttgtcgcg ccaccaattg
      181 caacggatat tcatcggcct tggtccagcc gctcgccgcg atcaacagca cgatgcagtc
      241 cacgccgtgc aggctggcgt cggccacgcg attcatatgg cgattcatca cgcccttatt
      301 atccgtatgc aggccgggcg tatccacata aacaatctgg gcctgttccg tggtcttgat
      361 gcccaacagc cggtggcgtg tcgtctgggc gcgggacgag gtaatgctga tcttctgccc
      421 gaccagccgg ttcaacagcg tggatttgcc tacgttcggt cgtccggcca gcgtcacgag
      481 tccgctgcgc gtggtcgcgt caggcatcag cgtcggtgca gcaactcaag ggcctgggcc
      541 gccgcttcct gttcggcatg acgacggctg ttgccctcgc cgcgcacggg ccgatcgagc
      601 accgtcacat ggcattccac cacaaagctc tgattgtgcg gttcgccggt gacgtcccgc
      661 acgagatagg tgggtgtcgg cagggaaagt ttttgcaaat attcctggag ctgggtcttc
      721 ggatccttgg ggatggaatg cggatccagc cccacgagct tgtcgtggta aagatgcaga
      781 atgacgcgca tggcttccgt caccgagctg tccttgcaga tggcgccgaa tattgcctcg
      841 agggcgtccg ccaggatcga gtcgcggtcg tatccaccgc tcttgagctc accctctccc
      901 agcttcagat aattccccag atcgaggttg cgggccagtg ccgccagggt ttccttcttg
      961 accaggctcg cacgcaggcg cgtcagctca ccttcgtaca agttcggata gcgattataa
     1021 agctcggagg aaatggcgaa acccagaacg ctgtcgccca gaaactcaag gcgttcataa
     1081 ttggcggcgc ttttgctgcg atgcgtcagg gcccgttcga gcagctcagg ttgccggaaa
     1141 gtatactcca accggcgcac gagctcggca ggcattttat tctgtgccac ggacctcgat
     1201 gctgtgatca aaagtaagca gcactgacac gttgtatagt atcggcgtca ctgattcata
     1261 attaatgcgt atgagcttgg ttcttccctt cgctgtaata gtgagcgatt tgctcagatc
     1321 gaaatcatcg gcgctgtcga tctcaagccg tttgcggatg gcttccgaga tttccgggac
     1381 agacatgctg ccagcatcag gctggcgacc cagactctcc agcgcgcttt tcaccttcat
     1441 gtcgcccagg tacggcggga taagcttgaa gagcagaaaa aggaagaatg ccaacgtgcc
     1501 gagcacaaat aacagactcc acatggtcat gcccgattgg cgttttgggt tttgcatcat
     1561 gatctccttc gcaatgccaa gccagatatg ctgttaataa atcgagttac cgacgcggtc
     1621 ccagacaatg cgctgccaga accatttttc ctgtccggtt acatcccagc tgaaccagat
     1681 caggaaagcc cttcccacca gattctggtc cggcacgaaa ccccaataac ggctgtcgtt
     1741 gctgtgatca cgattgtcac ccatgacgaa atatttcccg gcgggcacca cgatctccgg
     1801 cagcggagga tccatgcggt ttgtcgtcag aatgtcgtgc ttcacgccgt cgagactttc
     1861 catcagtcgc agcgcctcgc cctgggcgtc tcctcccgag gcgaagtaat aaggccttcc
     1921 atcggcttgt tccatgagcg tgccgttgat atagagcttc ttatctttat ataggatatg
     1981 gtcgccggga acgccaacaa tgcgctttat atagttgatc gaggggttcc ccggaaaacg
     2041 gaagaccgcc acctcccccc gcttcggctg acccgtttcc agaatcttgt tgttggcgac
     2101 gggcaggcgt accccgtagg aaaacttgtt cacctgattg aaatcgccga tgaacaggct
     2161 gggaagcatc gaaccggaag gaatgcgaaa cggctccacc aggaaggaac ggataaaaaa
     2221 tacaatcaga atgacg
//
LOCUS       DBNGZE010000133         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1199506, whole genome shotgun sequence.
ACCESSION   DBNGZE010000133 DBNGZE010000000
VERSION     DBNGZE010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1199506"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16FV_03380"
     CDS             complement(<1..695)
                     /locus_tag="AC16FV_03380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2849139.1"
                     /translation="MSEARLTSLTLDSSPPARVIAVRTREGKNPGLFWLGGFKSDMKG
                     TKAEALDRWADQEGRACVRFDYSGHGDSGGAFTDGTISRWLEESITVYVTFARGPQVI
                     IGSSMGGWLALLLARALCERQGAAPIAGMVLIAPAVDFTEELMWKQFPDSTKREIEQK
                     GVWMRPSEYGNDPYPITRGLIEDGRKHLLLGGLIETRCPVHILQGVQDPDVPWHHAVE
                     LVSRFSRDDVVITL"
     gene            702..>1770
                     /locus_tag="AC16FV_03385"
     CDS             702..>1770
                     /locus_tag="AC16FV_03385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2849140.1"
                     /translation="MDKYRMGSLLVAARCMSMQGGTVAAAIRDVYTSLLACSASNDYS
                     ICARKAPEFLGQRKDMSSLPASVLPTSSSSNGDVSENASRLPTAAKTTIAVLIVVPTL
                     DGGAADAGAVELVRILAGAGHRAIVLSRAGRLVADVTTAGGEFIPLDVSSNDPIVMLR
                     SAFVLLRLARERRCAAIHALGRAAAWSAFIAARLCGIPFVTSWYKGFREQNFFKRQYN
                     GIMARGDRIIASSEQIAQLINDRYATPWEKIIVVPCSIDLDRFNPESVTRERVEAMRA
                     AWGVKRDTKVILITGRILRRKGHHVVVKAVKRLKEMGLKDFLCVFVGEDRGRTRYTGE
                     LWDLVLTTGTMDVIRMAAPVAD"
BASE COUNT          358 a          520 c          528 g          364 t
ORIGIN      
        1 agagtaatca cgacatcatc gcgagagaag cgcgacacaa gttcgaccgc gtgatgccat
       61 ggcacgtccg gatcctgcac gccttgcagg atgtgcactg ggcaacgtgt ttcgatcagg
      121 ccgccgagca atagatgctt ccggccatcc tcaatcaaac cgcgcgtgat gggataaggg
      181 tcgttgccgt attcagatgg ccgcatccac acgccttttt gctcgatctc gcgcttggtc
      241 gaatcgggaa actgcttcca catcagctcc tcggtgaagt cgacggctgg tgcgatcagg
      301 accatgcccg cgattggcgc tgcgccttgc cgttcacaca aagcgcgcgc caacaacaat
      361 gccagccagc cgcccatgga tgagccgatt atgacctgcg gtcctcgggc gaaggttaca
      421 tagaccgtga tactttcctc cagccatcgg ctgatggttc cgtcagtaaa ggcaccgcct
      481 gaatcaccgt gaccggaata gtcgaagcgc acacaggcgc ggccttcctg gtcggcccag
      541 cggtcaagcg cctctgcttt cgtgcctttc atgtcggact tgaacccgcc cagccagaac
      601 aagcctggat tcttgccctc acgcgtccgc acggcgatga cccgggccgg tggagatgag
      661 tctaaagtaa gagaggttag gcgagcttca ctcatgcgtg tttggacaag taccggatgg
      721 gcagccttct tgtcgccgca agatgcatgt caatgcaagg gggaaccgtt gccgccgcca
      781 ttcgcgacgt ctatacctcg ctgctggcat gcagcgcgtc aaacgattac tctatatgcg
      841 ctcgaaaagc gccggaattt ctaggacaaa gaaaagatat gtcgagcctg cccgcatccg
      901 tattaccgac atcgagtagc tcaaatggcg atgtatcgga aaatgcatcg cgcctaccta
      961 cggccgcaaa aaccacgatc gctgttctca ttgtcgtgcc cacgctcgat ggcggtgccg
     1021 ccgatgcagg cgcggtcgag ttggtgcgta tccttgcggg agccggccat cgtgcgatcg
     1081 tgctgtcgcg cgcgggccgt ctcgtagcag acgtcaccac agccggtggc gagttcattc
     1141 cgctcgatgt ttcgagcaac gatcccatcg tgatgttgcg cagcgccttc gttcttttgc
     1201 gattggcgcg cgagcggcgg tgcgctgcaa ttcacgccct cggccgcgcg gcggcctgga
     1261 gcgcgttcat cgccgcgcgg ctgtgcggta ttccgtttgt gaccagttgg tacaaaggat
     1321 tccgtgagca gaattttttc aagcgtcagt acaacgggat tatggcacgc ggtgaccgta
     1381 tcatcgcatc aagtgagcaa atcgcgcagc tgatcaacga tcgttacgcc acgccctggg
     1441 aaaagatcat agtagtgccg tgcagtatcg atctggaccg tttcaatccg gaaagtgtca
     1501 cgcgcgagcg cgtagaggca atgcgtgccg cctggggcgt caagcgcgac accaaggtga
     1561 tcctgatcac cggccgcatt ctgcggcgca agggtcatca tgttgtcgtc aaggcggtga
     1621 agcgactcaa ggaaatgggc ctgaaggatt ttctgtgcgt cttcgtcggc gaggaccgtg
     1681 gccgcacccg ctacacaggc gaattgtggg atcttgtgct gacgacgggc accatggacg
     1741 tgatccgcat ggcggcgccc gttgccgaca
//
LOCUS       DBNGZE010000134         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_157640, whole genome shotgun sequence.
ACCESSION   DBNGZE010000134 DBNGZE010000000
VERSION     DBNGZE010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_157640"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1200)
                     /locus_tag="AC16FV_03390"
     CDS             complement(58..1200)
                     /locus_tag="AC16FV_03390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR2849141.1"
                     /translation="MVAGAILLALLTAVLSPAHAADGRGGEEERLKKLRVRIETLQEK
                     LNETRGRRDDVREEVRGLEQRIGTQMNDLRKTDAQLRTNEKKLADLNARAAREQGNLG
                     AQRQQLARQIYSAYLMGRQEYPKLLLNQENPARVARVVTYYEYLNQARTERISQIQTT
                     LSRLERLEQQIREQRRDLEGLRANQREQKAALETSRARRGELLASLNREVRDQSQRME
                     RLRADEKRLEQLIEELKTVLPDPPLPSGTTRFAKLRGRLPLPTRGGILARYGAQKNVG
                     NLKWRGLLIGGREGQNVISVYRGRVVFADWLRGFGLLLILDHGDGYMTLYGHNQSLHK
                     GVGDRVEAGEVIASLGNTGDMAQPGLYFEIRQNGEPRDPLIWCRAR"
     gene            complement(1275..>1938)
                     /locus_tag="AC16FV_03395"
     CDS             complement(1275..>1938)
                     /locus_tag="AC16FV_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964245.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR2849142.1"
                     /translation="PVAFPPERLRNVMGSYLASLGRHQLRIAETEKYAHVTFFFNGGV
                     ETPFEFEDRLLIPSPTDVPTYNLKPEMSAPKLTEEVVKAIRAGHYDVIICNYANPDMV
                     GHTGDFEATVKAIEAVDQSLSSIYKAVMETGGEMLITADHGNAEQMFDFETGQPHTAH
                     TTNPVPFLYIGRPATLAPSGALEDIAPTMLYLLGLPLPTEMTGRPLVALQSQQATAEG
                     HA"
BASE COUNT          333 a          562 c          599 g          444 t
ORIGIN      
        1 cggtgggcgg cgaaaaatac taatccattc gtcttgagat aataaacaaa gcggttgtta
       61 tcgtgccctg caccagatca gcggatcgcg tggttcgccg ttctggcgaa tttcgaagta
      121 aagtcccggt tgtgccatgt cgccggtatt gccgagactg gcgataactt cgcccgcctc
      181 gacccggtcg ccgactccct tgtgcaggct ttgattgtgg ccgtacagcg tcatatagcc
      241 atctccgtgg tccagaatca agagtagccc aaaaccccgt aaccagtcgg caaacaccac
      301 gcgtccgcga tagacggaaa tcacattctg gccctcgcgg ccgccgatga gcagaccgcg
      361 ccatttgaga tttcccacgt ttttctgggc accgtagcgc gcgagtattc caccgcgggt
      421 tggcaagggc aggcgccccc gcagtttggc gaagcgggta gtgccagatg gcagaggcgg
      481 gtcgggcaga accgttttca gttcctcgat cagctgttcc aggcgctttt catccgcgcg
      541 cagccgctcc atccgctgtg actggtcgcg cacctcgcgg ttcaggctgg cgagcagttc
      601 gccacggcgc gcgcgcgagg tttcgagcgc ggctttctgt tcgcgctgat tggcgcgcag
      661 gccttccaga tcgcggcgct gttcacgtat ctgctgttca agtctttcca gccgggaaag
      721 ggttgtctgg atctggctga tacgctcggt ccgcgcctgg ttcagatatt cataataagt
      781 aacgacacgc gcgacgcgcg ccgggttttc ctgattcagc agcaatttgg gatattcctg
      841 acgacccatg aggtaggctg agtaaatctg gcgcgccagt tgttgtcgtt gtgcgccaag
      901 attgccctgt tcccgggcgg ctcgggcatt caggtccgcg agtttttttt cgttggtgcg
      961 cagctgcgcg tcggtcttgc ggaggtcgtt catctgtgtg ccgatgcgtt gctccagccc
     1021 gcggacttcc tcgcgaacgt cgtcgcgccg gccgcgggtt tcattcagtt tttcctgaag
     1081 cgtttcgatg cggacacgca gtttcttcag ccgttcttcc tctccgccgc ggccatcggc
     1141 ggcatgggcc ggggagagaa cggcggtcag taatgcaagc agaatggccc cagcaaccac
     1201 ggttgtccgc ccggattggc aaccgttctt tttccgtttc ggcgttaaga cagcggaaga
     1261 tatcaccgcg tgcgtcaggc gtgaccctcg gcggttgcct gctggctttg caaggcaacc
     1321 agcggccggc cggtcatttc ggtcggaagg ggcagaccca ggagataaag catggtcggt
     1381 gcaatgtcct cgagcgcgcc gctcggtgcc agcgtggccg gtctgcctat ataaaggaat
     1441 ggcaccgggt tggtggtgtg tgccgtgtgt ggctggccgg tttcaaagtc gaacatctgc
     1501 tcggcgttgc cgtggtcggc ggtgatgagc atttcacccc cggtttccat gaccgccttg
     1561 tatatactgc tcaacgactg atctacggcc tcgatggcct ttacggtggc ttcaaaatca
     1621 ccggtatgcc ccaccatgtc gggattggcg taattgcaga tgatcacgtc gtagtgcccc
     1681 gcgcggatag ccttgaccac ttcttcggta agcttcggcg cactcatctc cggtttgaga
     1741 ttgtaggtgg gcacgtccgt cggcgagggg atcagcaagc gatcctcgaa ttcgaacggt
     1801 gtttccacgc cgccgttgaa gaagaaggtt acgtgcgcgt atttctcggt ttccgcgatg
     1861 cgcagctgat ggcggcccag actggccaga taggaaccca tcacgttgcg caggcgttcc
     1921 ggcggaaacg cgaccggc
//
LOCUS       DBNGZE010000135         1763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1407931, whole genome shotgun sequence.
ACCESSION   DBNGZE010000135 DBNGZE010000000
VERSION     DBNGZE010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1407931"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="AC16FV_03400"
     CDS             <1..522
                     /locus_tag="AC16FV_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR2849143.1"
                     /translation="MVGIALKTRPHAVCLVPEKRTERTTEGGLDAVKQRAQLAPVVRE
                     LKHAGIRVSLFIGAAPEQIEMAAELGAPVIEIHTGSWCDALTEGRSSEARAEWQLIRD
                     GAVLAASVGLEVHAGHGLDYGTAETIAALPQVVELNIGHFLIGEAVFEGLAETVKFMR
                     AAMDRGRKKASHP"
     gene            519..938
                     /gene="acpS"
                     /locus_tag="AC16FV_03405"
     CDS             519..938
                     /gene="acpS"
                     /locus_tag="AC16FV_03405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR2849144.1"
                     /translation="MIIGLGSDIVDVRRIADVIERHGERFLGRIFTTTERTKAEMRAN
                     TIETYAKRFAAKEACAKALGTGLRRGVFWRDMGVVNLAGGQPTMKLTGGALSRLQAIT
                     PPGFEARIDISLTDEGPTAQAIVIISAVPASSAGVSS"
     gene            1051..>1763
                     /gene="lepB"
                     /locus_tag="AC16FV_03410"
     CDS             1051..>1763
                     /gene="lepB"
                     /locus_tag="AC16FV_03410"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR2849145.1"
                     /translation="MSVTSGTKRKEGGIAETIRVVIHALIIALVIRTFLFQPFNIPSG
                     SRKATLLVGDYLFVSKYSYGYSKYSLPLSPPLFSGRIPGDWVPQRGDVVVFRLPKDPS
                     TDYIKRVIGLPGDKIQVIDGVVNINGAPVKRERASDFVETEEGTRDAPVKRWKEILPN
                     GVSYYVLDLVDNGFADNTQVYTVPPDHYFMMGDNRDNSTDSRFSQVGMVPFENIVGKA
                     QIIFFSVFEGERAWQFWRWP"
BASE COUNT          369 a          480 c          544 g          370 t
ORIGIN      
        1 atggttggca ttgcgctcaa gaccagacca cacgcggtct gtctggtgcc cgaaaagcgc
       61 actgaaagga ccaccgaagg tgggctcgat gccgtcaagc agcgcgcgca acttgccccc
      121 gtcgttcgtg aactcaagca cgccggcatc cgcgtttccc tttttatcgg agcggcgccg
      181 gagcagatcg aaatggcggc ggaactgggc gcacccgtga ttgaaatcca cacgggttcc
      241 tggtgtgacg cattaacgga agggcgaagc agcgaagcca gggcggagtg gcagctcatc
      301 cgcgatggcg cggtgctcgc cgcgagtgtg ggtcttgaag ttcatgcggg ccacggtctc
      361 gactatggga cggcggagac gatcgctgca ctgccgcaag tggtcgagct caacatcggc
      421 cacttcctga ttggtgaggc ggtgttcgag ggattggccg agactgtgaa attcatgcga
      481 gcggcgatgg accgtggccg taagaaagcg tcacacccat gattatcgga ctcggctctg
      541 acatcgtgga cgtacgaagg attgccgatg tgatcgagcg ccatggtgaa cgcttcctcg
      601 gtcgcatctt cacgacgacc gagcgcacca aggccgaaat gcgtgcaaat accattgaga
      661 cttacgccaa gcgttttgct gccaaggaag cttgtgccaa agctctcggt acggggctgc
      721 ggcgcggggt cttctggcga gacatgggtg tggtcaatct tgcgggcggg cagcctacga
      781 tgaagctgac cgggggcgct ctctcgcggc tgcaggcgat cacgcctcct gggttcgaag
      841 ctaggatcga tatttcgctg accgacgaag ggccaaccgc tcaggccatt gtcattattt
      901 ccgcggtgcc cgcatcgtca gcaggagttt cgagctgaag acaggaacct cagtccgacc
      961 aggaattttt cttgtggctc gggtcttgtt gattgcttgc cggggtgcca gccgctacaa
     1021 ggtcgccggg gcgcgcagga cgaaaggccg atgagtgtga catctggaac aaaacgaaaa
     1081 gaaggcggca ttgccgaaac aatacgcgtc gtcattcacg cgctgatcat tgcgctggtg
     1141 atccgcactt tcctgtttca gccgtttaat attccctccg gctccaggaa agcgacgctg
     1201 ctcgtcggcg actatctatt cgtttccaaa tatagctacg gttacagcaa atattcactg
     1261 ccgttgtcac cgccactgtt ttctggccgc attccaggtg attgggtgcc gcaacgcggc
     1321 gacgtggtgg tgttccgcct gccgaaagat ccttcgaccg attacattaa gcgggtgatt
     1381 ggcttgcctg gtgacaaaat tcaggtcatc gacggcgtcg tcaacatcaa cggtgcgccg
     1441 gtcaagcgcg aacgcgcatc cgatttcgtc gaaacggaag agggcacgcg ggatgcaccg
     1501 gttaagcgtt ggaaggaaat actgccaaat ggcgtgagct actatgtact cgatctggtg
     1561 gataatgggt ttgccgataa cacccaggtc tatacggtgc ctcccgacca ctatttcatg
     1621 atgggcgaca accgcgacaa ctcgaccgac agccgattca gccaggtggg aatggtgccg
     1681 ttcgaaaata tcgtgggtaa agctcagatc atcttcttct ccgtgtttga aggcgagcgc
     1741 gcctggcaat tctggcgctg gcc
//
LOCUS       DBNGZE010000136         5414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_852298, whole genome shotgun sequence.
ACCESSION   DBNGZE010000136 DBNGZE010000000
VERSION     DBNGZE010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_852298"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="AC16FV_03415"
     CDS             complement(<1..472)
                     /locus_tag="AC16FV_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HIR2849146.1"
                     /translation="MTQITIKGFADQIGIPPETLLRQLDAAGIAAKSADDALTEDEKA
                     KLFHYLRASHGAADEGAAKKKITLKRKSTSQVVQSTRTGASRTVQVEVRKKRTFIKRT
                     AVEETPSLAEEVPVEEPVEEAAPTPVVAKAPVQEPVTAEVTPAPVAEAPAAPKPV"
     gene            complement(495..1994)
                     /gene="nusA"
                     /locus_tag="AC16FV_03420"
     CDS             complement(495..1994)
                     /gene="nusA"
                     /locus_tag="AC16FV_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR2849147.1"
                     /translation="MGNEVLIIVDAVSREKGVDKEIIFQALEAALATATRKRHKGDID
                     ARVAIHRDTGAYDTFRRWEVIPDETEVVEFPDRQYKLTEARAIKPDIQVGEFIEEPLE
                     PVEFGRIAAQAAKQVIMQKVREAEREQIVNLYKDRKGEMVTGVVKRLERGDAIVDLGN
                     AEALLSKNTMIPREGLRPGDRIRALLEDVKSVPRGPQLFLSRVAPKLLVELFKLEVPE
                     AGEGLITIHGAARDPGLRAKIAVSSSDARIDPIGACVGMRGSRVQSVSNELAGERVDI
                     ILWSENPAQFVINALAPAEVQSIVVDEEVHSMDVIVDEKQLSQSIGRSGQNVRLASDL
                     TGWELNIMTAESAQEKGNQEAANAIKLFMEQLDMDENVATVLVQEGFTNLDEVAYVPK
                     QELLAIEEFDEELVDELRNRARDLLLTKAIAQEEKISLAEPAQDLLEMQGMDENTAHL
                     LAARGIKTMEELAEQAVDDLLEIDGMTDERARELIMTARAPWFEDKQAG"
     gene            complement(2026..2481)
                     /gene="rimP"
                     /locus_tag="AC16FV_03425"
     CDS             complement(2026..2481)
                     /gene="rimP"
                     /locus_tag="AC16FV_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR2849148.1"
                     /translation="MQTTRADLRKLLEPGVSAMGFELVDVEMAGSHHSPTLRVYIDSP
                     RGVNVDDCAKVSRQLSALLDVEDPLPGHYTLEVSSPGLDRPLVKPEDFKRFVGETIKV
                     KMQQPVLGRRNFSGRLVEVAADHVAVEVDKEIFNLAFDDMERARLVPRF"
     gene            complement(2671..2747)
                     /locus_tag="AC16FV_03430"
     tRNA            complement(2671..2747)
                     /locus_tag="AC16FV_03430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2711..2713),aa:Met,seq:cat)
     gene            complement(2810..3535)
                     /locus_tag="AC16FV_03435"
     CDS             complement(2810..3535)
                     /locus_tag="AC16FV_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849149.1"
                     /translation="MRTEGEFRLHAIVRAVLSPGIWFVLVAQIALMFALQWRAPVDAA
                     PLVSMLAIFLMATALMLFFYLQAGAFQALTLGREALRVGEIIRAGKTVFASFVWLTLK
                     AGLLFALVINVLVLVALLLTGMDFKSLMQTLSAFFGPMTGMLAFVFAYWLPFVFVRRE
                     FRLLPSLMAALQIARERLAHSAFLALLVLVPALAIVLLPADSPMWLDVLVSVATGIMG
                     WVAYIYCVDILRQRRPGAAAEHQ"
     gene            complement(3594..3866)
                     /locus_tag="AC16FV_03440"
     CDS             complement(3594..3866)
                     /locus_tag="AC16FV_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2849150.1"
                     /translation="MQIHVEHDPSPKKLEVIGVYDWPIWTRDVSKFPWTYDQKETCYI
                     LEGEIIVTPDGGEPVKIVENDLVSFPAGMSCTWEILSPVKKHYNFG"
     gene            4085..>5414
                     /locus_tag="AC16FV_03445"
     CDS             4085..>5414
                     /locus_tag="AC16FV_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2849151.1"
                     /translation="MISPRYSKDPRTEQSLRHSVRDGVAYSVMAGAGEHYLSAYALFL
                     KATTAQISWLAAFPSLFGSFAQLFSAWLASRIGRRQSIILFGVVLQTAMWLPIIWLPY
                     FFPAQAVMILIVCAALYYAGGHLATPVWNSLMGDLVPEKRRGRFFARRTRLMSMTSFL
                     ALVGAGLLLYGFEIAERVRFGFMLIFTVAALARLYSATQLARMHDPERVPVNVDWPAL
                     SGLWQRMRGSDCVRFTFFFAAMQFAAAIAAPFFAVYMLRDLGFSYLQYMSATALAVLA
                     QFGSLRMWGRLGDIFGNRVILTVTGSVIPFFPMLWLVSPHFAYVAVLQMFGGLFWAGF
                     SLSASNYLYDSVSPERRAAYSAVHNVFTSVAIFAGALLGGFLSLVVPENFVLFGKSWH
                     WTSSLWGVLVASTLARGMVALSLAPALREVREVRRLTAAGLILRVARFNAL"
BASE COUNT          968 a         1724 c         1587 g         1135 t
ORIGIN      
        1 gtacgggctt cggcgccgcc ggcgcttcgg caaccggggc cggcgtcact tcggccgtca
       61 ccggttcctg cacgggggct ttggcaacaa ctggtgtggg cgcagcttcc tcgacaggtt
      121 cctcaaccgg cacttcttcc gccagagaag gtgtttcttc cacggcggta cgcttgatga
      181 aagtgcgctt cttgcgcacc tcaacctgca ccgtgcggct ggcgccggtg cgcgtgctct
      241 gaacgacctg actggtggat ttgcgcttga gcgtgatttt cttcttggcc gcgccctcgt
      301 cggcagcgcc gtgactggcg cgcagataat gaaacaactt cgccttttcg tcttccgtca
      361 gcgcgtcgtc cgcgcttttg gccgcgatgc cggcggcatc gagctggcgc aacagcgtct
      421 cgggcgggat gccgatctgg tcagcgaagc cttttatcgt gatctgcgtc ataaactcct
      481 gaaaggcgtg gcgcttagcc cgcctgcttg tcctcgaacc acggcgcgcg cgccgtcatg
      541 atcagctcgc gggcgcgttc atccgtcatg ccatcgattt cgagcagatc gtccaccgcc
      601 tgctccgcca gttcttccat ggtcttgatg ccgcgggcgg ccagcaggtg cgcggtgttt
      661 tcatccatgc cctgcatttc gagcagatcc tgggccggct cagccaggct gatcttctct
      721 tcctgggcaa tggccttggt cagcagcagg tcgcgcgcgc ggttgcggag ctcatccacc
      781 agctcctcgt cgaattcctc gatggcaagc agttcctgct tcggcacgta ggcgacttca
      841 tccaggttgg tgaagccttc ctgcaccagc accgtggcga cgttctcgtc catatccagc
      901 tgttccatga acagcttgat ggcgttggcc gcttcctggt tgcctttttc ctgcgccgat
      961 tccgccgtca tgatgttgag ctcccatccg gtgagatcgg aagcgagccg cacgttctgg
     1021 ccgctgcgac cgatggactg cgacagctgc ttctcgtcca cgatcacgtc catgctgtgc
     1081 acttcctcgt ccaccacgat ggactgcacc tcggcgggcg cgagcgcgtt gatcacgaac
     1141 tgcgccggat tttccgacca caggatgatg tccacgcgtt caccggcgag ttcgttcgac
     1201 acgctctgca cgcgcgaacc gcgcatgccg acacaggcgc cgatgggatc gatgcgcgca
     1261 tcggaggaac tcaccgcgat tttggcgcgc agacccggat cgcgcgccgc gccgtgaatg
     1321 gtaatcaggc cttcgccggc ttccgggact tcgagcttga acagttcaac gagcagtttg
     1381 ggcgccacgc gtgacaggaa cagttgcggt ccgcgtggaa ctgatttcac gtcttccagc
     1441 agcgcgcgga tgcgatcgcc cggccgcaga ccctcacgcg ggatcatggt gttcttggag
     1501 agcaaggcct cggcattgcc caggtccacg atggcgtcgc cgcgctccag gcgcttgacc
     1561 acaccggtga ccatctcgcc cttgcggtcc ttgtagagat tgacaatctg ctcgcgctcg
     1621 gcctcgcgca ccttctgcat gatgacctgc ttggccgctt gcgccgcgat gcggccgaac
     1681 tcgaccggct ccagcggctc ttcgatgaat tcgccgacct gaatgtccgg cttgatcgcg
     1741 cgggcctcgg tcagcttgta ctggcggtcg gggaattcaa ccacctcggt ttcatcgggg
     1801 atgacctccc agcggcggaa ggtgtcgtaa gcgccggtgt cacgatgaat cgcgacgcgc
     1861 gcgtcgatgt cgcccttgtg gcgcttgcgc gtggcagtgg ccaaggccgc ttcgagcgcc
     1921 tggaaaatga tttccttgtc gacgcctttt tcgcgcgaca ccgcatccac aataatcaat
     1981 acttcgttgc ccatcatgct cgctatctcc cgtcacaccc gccgttcaga accggggcac
     2041 cagccgcgcc cgctccatgt catcgaacgc caggttaaaa atctctttgt ccacttccac
     2101 cgccacatga tccgccgcca cttccaccag tcgcccggag aaattccggc gcccgagtac
     2161 gggctgctgc atcttcacct tgatcgtctc gccgacgaac cgtttgaaat cctccggttt
     2221 gaccagcggc cggtcgagtc cgggcgagga cacctcgagg gtgtaatgtc ccggcagcgg
     2281 gtcctcgacg tccagcagcg cggacagctg ccggcttact ttggcgcagt cgtccacatt
     2341 cacgcctcgg ggactgtcta tatatacccg caacgtgggg ctgtggtggc tgccggccat
     2401 ttccacgtcc acgagttcga agcccatggc actgacaccg ggctcgagca gcttgcgcaa
     2461 atccgcccgc gtcgtctgca tattttccaa tcccataaaa cacaaagtgg gcgcgcggcc
     2521 cacttcttat tactcttgct tgctctggcg actgctgata ccggcttgaa ttataagcat
     2581 ttcccgcgtg ttcggaaagt accgccaacc caggctaacc ggcgctctgc cagaaataaa
     2641 aaaggccctt gcgaggcctt tctaaaaagt tggtagcggg ggcaggattt gaacctgcga
     2701 ccttcgggtt atgagcccga cgagctgcca gactgctcca ccccgcagca ggacgcgaag
     2761 tctaacggcg agtctggatt aattcaagga aaaataggta cttaaacgtt cactgatgtt
     2821 ctgcggcggc accgggccgg cgctgccgca aaatatcgac gcaatagatg taggcgaccc
     2881 agcccatgat gccggtagcc acgctcacca gcacgtcgag ccacatcggg ctgtcggcag
     2941 gcaacaggac gatggccaga gcaggcacga gcaccagcaa cgccagaaaa gcggaatggg
     3001 ccagtctttc ccgggcgatt tgcagcgcgg ccatgaggct gggcagcagg cgaaactccc
     3061 gccgcacgaa cacaaacggc aaccagtaag caaacacaaa ggcgagcatg cccgtcatgg
     3121 ggccgaaaaa agccgagagc gtctgcataa ggcttttgaa atccatgccc gtcagcaaca
     3181 aggcaacgag gaccagaaca tttatcacca gcgcgaacaa cagacccgcc ttgagcgtga
     3241 gccacacaaa actggcaaat accgtcttgc ccgcccggat gatttccccg actcgcagcg
     3301 cttctcgtcc cagcgtcagg gcctggaagg cgcctgcctg cagatagaaa aacagcatca
     3361 aggccgtggc catcaggaag atggccaaca tcgaaaccag cggcgccgcg tccactggcg
     3421 cgcgccattg caaggcaaac atcagggcaa tctgcgccac cagcacgaac cagatgcccg
     3481 gactcaacac ggcgcgaaca atggcatgca aacggaactc gccctcggtg cgcacggggc
     3541 tgtgagaaac ttcctgcatt agtcaggtat ttataaggaa aatttcggac aactcaaccg
     3601 aagttatagt gctttttgac cgggctcagg atctcccagg tgcaggacat gccggcggga
     3661 aaggagacca gatcattttc cactattttc accggctcgc cgccatcagg tgtcacgata
     3721 atctcgccct ccaggatgta gcaggtttcc ttctggtcat aggtccaggg aaacttggag
     3781 acgtcccggg tccagatcgg ccagtcatag acaccgataa cttccagctt cttgggtgac
     3841 gggtcgtgct caacgtgaat ctgcatcagg cttcaccttg tgtgataagc ggcccattct
     3901 acgtcggcaa gacaaacccc ggcaacgcac cgccggcgag ttggcgcctg atcggttctc
     3961 ccgggcagtt cgtcggatcg ggcggccggt ggacatcgga tgcgaaaatg acgtctatac
     4021 tgaaatagtg taaatcccgt gaaaggcaga cacggcctga ttctgccgcc gcgagctgtc
     4081 gagcgtgatt tccccgcgtt attccaagga cccgcgtacc gaacagtcac tgagacattc
     4141 ggtgcgcgat ggcgtggcct attccgtcat ggccggcgcc ggcgaacact acctctcggc
     4201 ttacgccctg ttcctgaaag ccacgaccgc ccagatttcc tggctcgcgg ccttcccatc
     4261 gctgttcggc tcgttcgcac aattgttctc ggcttggcta gcgagccgca tcggccggcg
     4321 gcagtccatc atcctgtttg gcgtggtgct gcaaaccgcg atgtggctgc cgatcatctg
     4381 gctgccgtat ttcttccccg cgcaggccgt catgattctc atcgtctgcg ccgcgctgta
     4441 ttacgccggt ggtcatctgg cgacgccggt gtggaacagc ctcatgggcg atctcgttcc
     4501 cgaaaaacga cgcgggcgct tcttcgcgcg ccgtacccgt ctcatgagca tgacgagctt
     4561 tctggcgctg gttggcgccg gcctgctgtt gtacggtttt gaaattgcgg aacgcgtgcg
     4621 cttcggtttc atgctgatct tcaccgtggc ggcgctggcg cgtctctact ccgccaccca
     4681 gctcgcgcgc atgcacgatc cggagcgcgt cccggtcaac gtcgattggc cggcattgtc
     4741 cggcctgtgg cagcggatgc gcgggtccga ttgcgtgcgc ttcacttttt tctttgccgc
     4801 catgcagttc gcggcggcga tcgcggcgcc gttcttcgcg gtttacatgc tgcgcgatct
     4861 cgggttctcc tacctccagt atatgtcggc cacggcgctc gccgtgctgg cacagttcgg
     4921 cagcctgcgc atgtggggcc ggctcggcga catcttcggc aatcgcgtga tactgaccgt
     4981 caccggttcc gtgattccgt ttttccccat gctgtggctg gtatcgccgc atttcgccta
     5041 tgtcgcggtg ctgcaaatgt tcggcgggct gttctgggct ggcttcagcc tgagcgcgag
     5101 caactacctg tacgacagcg tgagccccga acgccgcgcc gcctacagcg ccgtgcacaa
     5161 tgtgttcacc agtgtcgcca ttttcgccgg cgccctgctc ggcggtttcc tgagtctcgt
     5221 ggttccggaa aatttcgtcc tgttcggaaa atcctggcac tggaccagca gcctgtgggg
     5281 cgtgctggta gcatcgactc tggcgcgggg aatggtggcg ctgtcgctgg cgccagcctt
     5341 gcgtgaggtg cgcgaagtgc gtcgccttac tgcggcagga ctgatattac gggtggcgcg
     5401 gttcaacgcg ctga
//
LOCUS       DBNGZE010000137         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1130399, whole genome shotgun sequence.
ACCESSION   DBNGZE010000137 DBNGZE010000000
VERSION     DBNGZE010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1130399"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..458)
                     /locus_tag="AC16FV_03450"
     CDS             complement(15..458)
                     /locus_tag="AC16FV_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR2849152.1"
                     /translation="MKWVTRERPKIDRIACPWLIARFIDKAPEFLYVPADEVMNVAQA
                     QGAMPYDVPGVELGHVGELCSFDAFLKKYRLTDQALQRLAVIVRGADTGRPALTPQSH
                     GLLAISLGLSHNFADDHEMLAHGMVMYDALYAWCRELPGAGDGRR"
     gene            complement(472..795)
                     /locus_tag="AC16FV_03455"
     CDS             complement(472..795)
                     /locus_tag="AC16FV_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2849153.1"
                     /translation="MNRSISPDDLKQLLYLRKNVLVLDVRRKSDYQTDGQKIPNAAWL
                     DPDKLTQWSTALPLDRDIVIYCVRGGSVSNSVVDQLQAKGVKARFIEGGIEAWKNAGG
                     DVVSK"
     gene            complement(896..2290)
                     /gene="chrA"
                     /locus_tag="AC16FV_03460"
     CDS             complement(896..2290)
                     /gene="chrA"
                     /locus_tag="AC16FV_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR2849154.1"
                     /translation="MPVESKTADLSRPPAAVSFGEAFSYWLKLGFVSFGGPAGQISMM
                     HQELVERRRWISEHRFLHALNYCMLLPGPEAQQLATYIGWLMHGVKGGIAAGVLFVLP
                     SLFILIALTWIYLAFGNVPAVAGILYGIKPAVTAIVVFAAYRIGSRALKNAVLWALAA
                     VAFVAIFAFNVPFPYIVLGAAILGFIGGRIAPGKFKTGGHHGDGGKSFGPALIDDHTP
                     PPEHTRFKWARVITFAVVGLAIWAGVMGLMFAQYGWEGTLTQMGWFFTKAALLTFGGA
                     YAVLPYVYQGGVDQHQWLTGTQMIDGLALGETTPGPLIMVVAFVGFVGGWTKEVFGPE
                     MLAVAGIAGATVATFFTFLPSFLFILLGGPAVDATRHDIKFTAPLIAITAAVVGVVLN
                     LAVFFAYHVLWPEGFPAPLSLQTLFDGFDWLPVIIGIAAFIALWRFKIGIIPVILACG
                     AVGLIYAFARPLFG"
     gene            2289..>2481
                     /locus_tag="AC16FV_03465"
     CDS             2289..>2481
                     /locus_tag="AC16FV_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849155.1"
                     /translation="MFFSTKLLTACYRKKEKTSDVVRCALHGDAIPRAVFLYGNVYVY
                     SRAGVLPPVVLFLFLEALGI"
BASE COUNT          573 a          747 c          696 g          465 t
ORIGIN      
        1 ttgccgatgt cgcgtcagcg ccggccgtcg cccgcgcccg gcagctcgcg gcaccaggca
       61 tagagcgcgt cgtacatcac catgccgtgc gccagcatct cgtggtcgtc ggcaaagttg
      121 tgcgacaggc cgagcgagat ggcaagcagg ccatgcgatt gtggcgtaag cgccggccgg
      181 ccggtatcgg cgccgcgcac gatcaccgcc agccgctgca gggcctggtc ggtgagccgg
      241 tatttcttca ggaatgcatc gaatgagcag agttcgccaa catgcccgag ctcgacgccg
      301 ggcacatcat aaggcatggc gccctgtgcc tgcgccacgt tcattacctc gtccgccggt
      361 acatagagaa actcgggggc cttgtcgatg aaccgcgcga tcagccaggg gcaggcgata
      421 cggtcaatct ttggacgttc gcgcgtaacc catttcatcc tgtcagctcc atcatttgct
      481 caccacatca ccacctgcgt ttttccaggc ttcgattcca ccttcgatga agcgcgcctt
      541 gacacccttt gcctgcaact gatcaaccac tgagttactc accgaaccgc cacgcacgca
      601 atagatgacg atatcccggt cgagcggcag cgccgtgctc cactgcgtga gcttgtccgg
      661 atcgagccag gcggcgttgg gaattttctg gccgtcagtc tggtaatcgc ttttgcggcg
      721 tacatcaagc acaagcacgt tcttgcgcag gtataaaagc tgtttgaggt cgtcgggcga
      781 tatgctgcgg ttcatgaaat ctccttgcgg tgccggcggc gtgatgaaca ggccgctgcc
      841 ttgtctgact taatttatag tatgcacgac acgcagattc catgggcgac gacactcaac
      901 cgaatagcgg ccgggcaaat gcgtagatca gccccacggc accacaggca aggattaccg
      961 ggataatccc gattttgaag cgccacaacg cgataaaggc ggcgatgccg ataattaccg
     1021 gcaaccagtc aaaaccgtcg aacaaggttt gcagacttag cggcgccggg aatccttccg
     1081 gccacagcac gtggtaagca aaaaataccg cgagattaag caccacaccc accaccgccg
     1141 cggtaatggc gatcagcggc gcggtgaact tgatatcatg acgtgttgca tccaccgccg
     1201 gaccaccgag aagtatgaac aggaacgacg gcaggaaggt gaagaaagtc gccaccgtgg
     1261 ctccggcgat tcccgccacg gccagcatct cgggaccgaa cacttccttg gtccagccac
     1321 ccacaaagcc gacaaaagcc accaccataa taagcggtcc gggggtggtt tcacccaatg
     1381 ccagaccgtc gatcatctgc gtaccggtca accactgatg ctgatccaca ccaccctgat
     1441 acacataggg cagcacggca taggcgccac cgaacgtgag cagcgcagcc ttggtaaaaa
     1501 accaccccat ttgcgtgagt gttccctccc aaccgtattg tgcaaacatc agacccatga
     1561 caccggccca gattgccaga cccacgacag caaaagtaat gactctggcc catttgaaac
     1621 gggtgtgttc cggtggcggt gtgtgatcat caatgagcgc cgggccgaag cttttgccgc
     1681 cgtcgccatg atggccgccg gttttgaact tgcccggggc aatgcgtcca ccgatgaaac
     1741 ccagaatggc cgcacccagc acgatgtacg gaaacgggac attgaaggcg aaaatcgcca
     1801 cgaaagccac agccgccagc gcccacagga ccgcattttt aagcgcgcgt gaaccgatac
     1861 gataggccgc aaaaacgaca atggcggtca ccgcgggttt gatgccataa agaatgccgg
     1921 cgacggcggg cacattcccg aaagcaagat agatccaggt aagcgcaatt agaataaaca
     1981 gcgacggcaa cacaaataaa acgccggcgg caatgccgcc tttcacgccg tgcatcaacc
     2041 agccgatgta ggttgccagc tgttgcgctt ccggtcccgg cagcagcatg cagtagttca
     2101 gcgcgtgcag gaagcgatgc tcggaaatcc agcgtcggcg ctcgaccagt tcctgatgca
     2161 tcatgctgat ctgcccggcg ggaccgccga agctgacgaa tccgagtttc agccagtagg
     2221 aaaacgcctc gccgaacgaa accgcggccg gaggccggga caggtcggca gttttcgatt
     2281 cgacaggcat gttcttctcc acaaaattat tgacagcttg ttatcgaaaa aaagaaaaaa
     2341 ctagcgacgt cgtccggtgc gccctccatg gcgacgcgat tccgcgcgcg gtgtttctgt
     2401 acgggaacgt ttatgtctat tctcgcgcgg gcgttttgcc gccggtggtt ttatttttgt
     2461 ttttagaggc gttggggatt t
//
LOCUS       DBNGZE010000138         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_783434, whole genome shotgun sequence.
ACCESSION   DBNGZE010000138 DBNGZE010000000
VERSION     DBNGZE010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_783434"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="AC16FV_03470"
     CDS             <1..1769
                     /locus_tag="AC16FV_03470"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2849156.1"
                     /translation="AVRGRRMAMIFQEPQTSLNPVLTIGEQISEAIPEADRLSRQERR
                     ARVAELLKAVGIADAARRYDEYPFQLSGGMKQRVMIAMALATQPDLLIADEPTTALDV
                     TIQAQILTLLKELQKKTGMAMLLITHDLGVVAETADRVAVMYAGQIVELAPRNKFFSA
                     PQHPYSRKLFRSIPGRMQRGAALEVISGSVPSLRQEFRSCRFADRCDRAWDLCRQKLP
                     VWIEEGDRGVRCHLYDETVSAPVAPPVSRRKARKTPSVTSGKAGKALLQVRDLKVYFP
                     IHKGVFQRVVGHVKAVDGVSLDIARGRTLALVGDSGCGKTTVGKGILQLVPPTGGHVL
                     YDNRELTQLKGEELRALRRHFQIIFQDPYASLNPRMLVGEILEEGMRAQSIGASRTER
                     QARVAELLQQVGLPADARQRYPHEFSGGQRQRISIARALAVEPELIVCDEPTSALDVS
                     VQAQVLNLLKRLQDELGLSYLFITHNLSVVEYLAHEVAVMYLGRIVERGPVDEVLENP
                     KHPYTEALLSAVPVIDPREKRTIIRLQGDMPSPSNPPSGCHFHPRCPHAMDICRREYP
                     ATTKFDNAHEARCHLFTTEKKN"
BASE COUNT          369 a          594 c          634 g          348 t
ORIGIN      
        1 gcgccgtccg cggcaggcgt atggccatga tttttcagga accgcagacc tcgctcaatc
       61 cggtgctgac gatcggcgaa cagatcagcg aggcaattcc ggaagcagac aggctcagtc
      121 ggcaagagag gcgcgcgcgc gtcgccgaac tgctcaaggc ggtcggcatt gccgatgccg
      181 cgcggcgcta cgatgaatat ccgttccagc tttccggcgg catgaagcag cgcgtcatga
      241 tcgccatggc gctggccacg caacctgact tgttaattgc cgacgagccg accacggcgc
      301 tcgacgtcac aatccaggcg cagattctga ccttgctcaa ggagcttcag aaaaagaccg
      361 gcatggccat gctgctcatc acgcatgatc tcggggtggt ggccgagacg gcggaccggg
      421 tggcggtgat gtacgcggga cagatcgtcg aactcgcgcc gcgtaataaa ttcttctccg
      481 cgccgcaaca tccctattcg cgcaagctgt tccgttccat cccgggccgg atgcagcgcg
      541 gcgcggcgct cgaagtcatc agcggcagcg tgccttcgct ccggcaggaa tttcgcagtt
      601 gccgcttcgc cgatcgctgt gaccgggcct gggatctgtg ccgtcagaaa ctgccggtct
      661 ggatcgagga aggcgatcga ggcgtgcgtt gtcatctgta tgatgaaacg gtttcagcac
      721 cagttgcgcc gccggtatcg cgccgcaagg cccgcaaaac tccgtccgtc acatcgggca
      781 aagccggcaa ggcgttgttg caggtgcgtg acctcaaggt gtatttcccg atccacaagg
      841 gtgtgttcca gcgcgtggta ggccatgtga aggcggtgga cggagtgtca ctcgacatcg
      901 cgcgcgggcg cacgctggcg ctggtgggcg actccggctg cggcaagacc accgtcggca
      961 agggcatact ccagttggtg ccacccacgg gaggccatgt tttgtacgac aaccgcgagc
     1021 tgacgcagtt gaaaggcgag gagttgcgcg ccttgcggcg ccacttccag atcatcttcc
     1081 aggaccctta tgcctcgctc aacccgcgca tgctggtggg cgagatcctg gaagagggta
     1141 tgcgcgccca gagtatcggc gccagccgta cggagcgcca ggccagggtg gcggagctgc
     1201 tgcaacaagt ggggctgccg gcggacgcgc gccagcgcta tccgcatgag ttctccggcg
     1261 gccagcgcca gcgcatcagc attgcccggg cattggcggt cgagccggaa ctgatcgtgt
     1321 gcgacgagcc caccagcgcg cttgatgtgt cagtgcaggc gcaggtgctg aacctgctca
     1381 aacgcctgca ggacgagctg ggcctgagtt acctgttcat cacgcacaac ctgtcggtgg
     1441 tggaatacct ggcgcacgag gtggcggtca tgtatctcgg ccgcatcgtg gagcgcgggc
     1501 cggtggatga ggtgctggaa aacccgaaac atccctacac cgaggcgctg ttgtcggcgg
     1561 tgccggtgat cgacccgcgc gagaaacgca cgatcatccg gctgcaaggc gacatgccgt
     1621 cgcccagcaa cccgccttcg ggctgccatt tccatccgcg ttgcccgcat gcgatggata
     1681 tctgccgtcg ggaatatccc gcgacgacga agttcgacaa cgcgcacgag gcgcgctgcc
     1741 atctgttcac gacggagaaa aagaattaag ccggagaaaa acggctaacc gttcggcgtg
     1801 ggattctcga ttgaagcggt gggcgcgcca ttgggccaga ttttcagcct ctgtcccatg
     1861 cgcagcatac tgttcgcgct gatgaaattc catttggcca gctgccgcac cagcacgttg
     1921 taacgccgcg cgatgctcga caggg
//
LOCUS       DBNGZE010000139         8283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_783536, whole genome shotgun sequence.
ACCESSION   DBNGZE010000139 DBNGZE010000000
VERSION     DBNGZE010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_783536"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /gene="acs"
                     /locus_tag="AC16FV_03475"
     CDS             <1..1735
                     /gene="acs"
                     /locus_tag="AC16FV_03475"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR2849157.1"
                     /translation="EGGKLNVSWNCLDRHLEKRGNQVAILWEGDDPREDRKITYRELH
                     EEVCRFANVLKSRGVKKGDRVCIYMPMIPESAVAMLACARIGAVHSVVFGGFSPQSLK
                     DRILDSDCRVVITTDEGVRGGRKVPLKHNTDEALAQCPNVHTVIVIRHTGADVPWKNG
                     RDIVCREALAKASADCPAEEMDAEDPLFILYTSGSTGKPKGVLHTSGGYLTYAAMTHK
                     YVFDYHDGDIFWCTADVGWVTGHTYIVYGPLCNGAITLMFEGVPTYPNASRFWEVIDK
                     HKVNIFYTAPTAIRALMREGDAPVKKTSRASLRLLGTVGEPINPEAWEWYYHVVGDAR
                     VPVVDTWWQTETGGILITPLPGATKLKPGSATRPFFGVVPGIVDLGTGKLLEGPAEGA
                     LVIQRPWPGQMRTVYGDHQRFKETYFSQYPGYYFTGDGCLRDQDGYYWITGRIDDVLN
                     VAGHRIGTAEVESALVLHEAVAEAAVVGFPHDIKGQGIYCYVTLMKGYESTEDLRKAL
                     VQQVRKEIGPTATPDVIQWAPGLPKTRSGKIMRRILRKIAANELDNLGDTTTLADPSV
                     VDDLVKNRAHQ"
     gene            1868..2698
                     /locus_tag="AC16FV_03480"
     CDS             1868..2698
                     /locus_tag="AC16FV_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2849158.1"
                     /translation="MSQSRNDAAGKEVLVDIRDVRLALGGREVLKGITLSIARGKVTA
                     IMGGSGCGKTTLLNLIGGRLRPDSGQVIVDGQSVPSLKTKELFELRKRMALLFQTGAL
                     FTDLTVFENVAFPIREHTRLPEPILRKVVLMKLETVGLRGARHLMPSELSGGMARRVA
                     LARAIVMEPMMIMYDEPFTGLDPISKGVIAKLIRSLNQTLGITSIVVSHDVPETCSIS
                     DYAYLVGEGRIIGQGTPAEVQQSGSEHVRQFMDGLPEGPVPYQYPASDYVQDLMQSTL
                     "
     gene            2695..3474
                     /gene="mlaE"
                     /locus_tag="AC16FV_03485"
     CDS             2695..3474
                     /gene="mlaE"
                     /locus_tag="AC16FV_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR2849159.1"
                     /translation="MNTLRALGGIVINTVERLGRASTFLYEVVTNSWELVRRFYLVIQ
                     QVYAVGVLTLLIILVSGLFVGMVLGLQGYNTLVKFGSEDTLGLLVALSLVRELGPVVT
                     ALLFAGRAGSALTAEIGLMKATEQLAGMEMMAVNPITRVIAPRFLGGIIAMPLLAALF
                     SAIGVLGGHLVGVGLLGVDSGAFWSQMQNKVDFYDDIVNGVIKSAVFGVVCTWIAVFE
                     GYDAVPTSEGVASATNRTVVYSSLAVLGLDFILTALTLGEV"
     gene            3476..3952
                     /gene="mlaD"
                     /locus_tag="AC16FV_03490"
     CDS             3476..3952
                     /gene="mlaD"
                     /locus_tag="AC16FV_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR2849160.1"
                     /translation="MINRKSLELWVGLFVAAGILALAMLAFKVGNLTTADVVDGYRVK
                     ANFDNVGGLKVKAAVTVAGVRVGRVTGIAFDSNKYQAVVTMDIGGQYKNIPVDSSATI
                     LTSGLLGDQYIGLEPGGAEEYLKDGDAIRTTQSALVLEKLVGQVIFNKGNEGGAAK"
     gene            3977..4630
                     /locus_tag="AC16FV_03495"
     CDS             3977..4630
                     /locus_tag="AC16FV_03495"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2849161.1"
                     /translation="MKRFVIVMLVLLLAPLAPAPAADVPPDQLARETTDKIVVLLKAN
                     KDAYSKDHKKLYVMVDEHVLAHFDFRAMSRTVLGRHWRAANEDQRTKFTTEFRELLVR
                     TYATALLKYNDEQIVYLPFRMSPEDRTATVKSEVRRKDGSPPIAIQYSFYHTDKGWKV
                     YDVAIEGASLVSTYQSTFSARVQKEGLDALIAGLAQDNKAAATGKTASNGGKTSVTR"
     gene            4627..4929
                     /locus_tag="AC16FV_03500"
     CDS             4627..4929
                     /locus_tag="AC16FV_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR2849162.1"
                     /translation="MSENGFASRGGGTFEMSGRLTFQTVPQFQAHAGSLFQGDTQPVT
                     IDMRGVTLADSAGLALMIEWLQLARAAKRQLVFTNIPEQVHHLIRVNGLTQVFALK"
     gene            4972..5889
                     /locus_tag="AC16FV_03505"
     CDS             4972..5889
                     /locus_tag="AC16FV_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2849163.1"
                     /translation="MSAISISGLHKRYPRVHALDGIDLTVERGEFFGLLGPNGAGKST
                     LINIVAGLTRFDAGRVSVLGQDVVTAYRQTRRLIGVVPQELTYDPFFNVREVLRIQSG
                     YFGLHKNDAWIDELLAALMLTEKAGANMRALSGGMKRRVMIAQALVHRPEVVVLDEPT
                     AGVDVELRKSLWTFIRRLHSEGHTIVLTTHYLEEAEALCHRIAILNRGKLVALDTKQA
                     LLARGIGRTRRVCINTERPLASLPGELEQKVRRRDGNMIELQLDKGTDSVMDTLDTLR
                     AHGAVITDLNMEGADLEDVFVELTRRDSA"
     gene            5892..6647
                     /locus_tag="AC16FV_03510"
     CDS             5892..6647
                     /locus_tag="AC16FV_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2849164.1"
                     /translation="MIGFYTLFYKEVLRFGKVLLQTLIAPVITALLYLLVFGQVFEGR
                     VQAFSGVSYTMFLIPGLVMMSVIQNAFANSSSSLIQSKVTGNIVFMLLTPLSHLEFFL
                     AFVLASVVRGILVGTGVYLVALVFTTVPVKHPGFILLFMFISSATLGAIGVIAGIWAE
                     KFDQLAGFQNFIILPLSFLSGVFYSIHSLPEFWQKLSHFNPFFYMIDGFRYGFFGVSD
                     VAPSHSLAIVTLFLVVLSGISLVLLRTGYKLRD"
     gene            6798..7022
                     /locus_tag="AC16FV_03515"
     CDS             6798..7022
                     /locus_tag="AC16FV_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR2849165.1"
                     /translation="MMQPDDIKQMIERGLPGARVSVTGDGHHFEAEVIASEFAGKSTL
                     QQHQIVYRTLGNKVGNEIHALSLRTQAPGE"
     gene            7072..7386
                     /gene="grxD"
                     /locus_tag="AC16FV_03520"
     CDS             7072..7386
                     /gene="grxD"
                     /locus_tag="AC16FV_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR2849166.1"
                     /translation="MNVQDKIREQLSGDKIVLYMKGTPQFPQCGFSAKAAQMLGACGA
                     KYASYDILMDPEIRQGLKEYSNWPTFPQLYVNGELVGGCDIMTELFQKGELQKLVAKA
                     MG"
     gene            7461..>8283
                     /locus_tag="AC16FV_03525"
     CDS             7461..>8283
                     /locus_tag="AC16FV_03525"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR2849167.1"
                     /translation="MDKLIVTGGSALRGEIKISGAKNAALPVLVASLLTNETVSIGNV
                     PHLQDITTTMELLGRMGVRLVVGDKMVIEADSSQNTSVRAPYELVKTMRASILVLGPL
                     LARFGEAEVSLPGGCAIGSRPVNLHIKGLQAMGAEINLDGGYIRAKAKRLKGARIFMD
                     LVSVTGTENIMMAATLADGMTIIENAAREPEVADLARCLNSMGARITGAGTDEIAVEG
                     VERLHGAVHEVIPDRIETGTYLVAGAMTGGSVRLRNARPDLLQAVLDKLRETGAVI"
BASE COUNT         1586 a         2444 c         2613 g         1640 t
ORIGIN      
        1 cgagggcggc aagcttaacg tttcctggaa ttgcctcgac cgccatcttg agaagcgcgg
       61 caatcaggtg gcgattctgt gggaaggcga tgacccgcgg gaagaccgca agatcactta
      121 ccgcgagctg cacgaggagg tttgcaggtt cgcgaacgtg ctgaagtccc gcggcgtcaa
      181 gaagggcgac cgggtatgca tctacatgcc gatgatcccg gagtcggcgg tggcgatgct
      241 cgcctgcgcg cgcatcggcg cagtgcactc ggtggtgttc ggcggtttct cgccgcagtc
      301 gctcaaggac cggattctcg actccgactg ccgcgtggtg atcaccaccg acgagggcgt
      361 gcgcggtggc cgcaaggtgc cgctcaaaca caacaccgac gaggcgcttg cccaatgccc
      421 gaacgtgcat accgtcatcg ttatccgcca caccggcgcc gacgtgccgt ggaagaacgg
      481 ccgcgacatc gtatgccggg aggcgctcgc caaggcgtcc gccgactgcc cggccgaaga
      541 gatggacgcc gaggacccgc tgttcattct ctatacctcg ggctcgaccg gcaagcccaa
      601 gggcgtgctg cacacgagcg gcggttatct cacctacgcc gccatgaccc acaaatacgt
      661 gttcgactat cacgacggcg acatcttctg gtgcaccgcc gacgtcggct gggtcacggg
      721 acacacttat atagtctacg gccccctgtg caacggcgcg attacgctca tgttcgaagg
      781 cgtgccgacc tatcccaacg cctcgcgctt ctgggaggtg atcgacaagc acaaggtaaa
      841 tatcttctat accgcgccaa cggcgattcg tgcgctgatg cgcgagggcg atgcgccggt
      901 gaagaagacc tcgcgcgcct cgctcagact gctcggcacc gtgggcgagc cgatcaaccc
      961 cgaggcttgg gagtggtact accacgtggt cggcgatgcg cgcgtgccgg tcgtcgatac
     1021 ctggtggcag accgagaccg gcgggatttt aatcacgccg ttgccgggcg ccacgaagct
     1081 taagcccggc tcggcgacgc ggccgttctt cggcgtcgtg cccggcatcg ttgaccttgg
     1141 caccggcaag ctgctggagg gtccggcgga gggtgcgctg gtgatccagc gcccctggcc
     1201 gggacagatg cgcacggtgt acggcgatca ccagcgcttc aaggaaacct atttcagtca
     1261 gtacccaggt tactacttca ccggcgatgg ttgtttgcgc gatcaggacg gttactactg
     1321 gatcaccggc cgcatcgacg acgtgctcaa tgtcgccggt catcgcatcg gcaccgccga
     1381 ggttgagtcg gcgctcgtgc tgcacgaggc tgtggccgag gcagcggtgg tcggtttccc
     1441 gcatgacatc aaggggcagg gcatctactg ctacgtcacg ctgatgaagg gttacgaatc
     1501 caccgaggac ctgcgcaagg cgctggtgca gcaggtgcgc aaagagatcg gaccgaccgc
     1561 cacgcccgac gtcatccagt gggcgccggg cctgcccaag acccgctccg gcaagatcat
     1621 gcgccgcatc ctgcgcaaga tcgcggccaa cgaactcgac aatctcggcg acaccacgac
     1681 tctggccgat ccttcggtgg tggacgatct ggtcaagaat cgcgcgcatc agtagcgcac
     1741 cgtcggtgcg gacgatacgc actgacgaat ggctgactat aaaaggctga caagggtcgc
     1801 ccggattgaa tagaatgtgt cgccctcaac cggacgaacg gcccggaatc gcaaaaagga
     1861 aagcggattg agtcagtccc gtaacgatgc agccggcaag gaagtcctgg tggatatccg
     1921 ggacgtgcgc ctcgcgctag gtggccggga agtgctcaag gggatcacgc tctccattgc
     1981 gcgcggcaag gtcactgcta tcatgggcgg cagcggttgc ggcaagacca cgctgctcaa
     2041 tctcatcggc ggacgtttga ggcccgattc cggccaggtc atcgtggacg gccagtcggt
     2101 gccgagcctc aagacaaaag agcttttcga gctgcgcaag cgcatggcgc tgctgttcca
     2161 gactggcgcg ctgtttaccg accttactgt ttttgaaaat gtcgcctttc ccatccgcga
     2221 gcacacccgg ctccccgaac cgatcctgcg caaggtggtg ctgatgaagc tcgagaccgt
     2281 gggcctgcgc ggcgcgcgcc acctcatgcc ttcggagctt tcgggcggca tggcacggcg
     2341 cgtagcactc gcgcgcgcga ttgtcatgga acccatgatg atcatgtacg acgagccgtt
     2401 taccgggctc gatcccattt ccaagggtgt gatcgcaaag ctcatccgca gccttaacca
     2461 gacgcttggc atcacctcga tcgtcgtgtc gcacgatgtc cccgagacct gctccatttc
     2521 cgactacgcc tacctcgtgg gcgagggccg catcatcggc caaggcacgc cggccgaagt
     2581 gcagcagtcc ggttcggagc acgtgcggca gttcatggac ggcctgccgg aaggtccggt
     2641 gccgtaccaa tatccggcga gcgattacgt gcaagacctg atgcagagca ctttatgaat
     2701 accttgcgcg cactcggtgg catcgtgatc aatactgtcg agcgcctcgg ccgtgcatcg
     2761 acgtttctgt atgaggtggt taccaactcc tgggagcttg tccggcggtt ttatctcgtt
     2821 atccagcagg tctacgccgt cggcgtgttg acgcttctta ttattctggt atccggcctg
     2881 ttcgtcggca tggtactcgg gctgcagggt tacaacacgc tggtcaaatt cggctccgag
     2941 gacacactcg ggctgctggt ggccttgtcg ctggtgcgcg aactcgggcc ggtggtgacg
     3001 gcgctgctgt tcgctggccg cgccggatcg gcgcttaccg ccgagatcgg tctgatgaag
     3061 gcgaccgaac agctcgccgg catggaaatg atggcggtca accccattac gcgcgtgatc
     3121 gcgccgcgct ttctcggggg catcatcgcc atgccgctgc tggcggcgtt attttccgcc
     3181 attggcgtgc tcggcggcca cctggtaggc gtggggttgc tcggggtgga ctccggggcg
     3241 ttctggtcac aaatgcagaa caaggtggat ttctacgatg acattgtgaa tggtgtcatc
     3301 aaaagcgccg tgttcggtgt tgtctgcacc tggattgcgg tattcgaggg ctatgacgca
     3361 gtgccaacgt cggagggcgt ggcgagcgcc accaaccgca cggtagtata ttcctcgctc
     3421 gcggtactag gcctggattt catccttacc gccttgacac tgggagaagt ctgaaatgat
     3481 caacagaaaa agtctggaat tgtgggtcgg gctgttcgtg gcggcgggaa tcctggcgct
     3541 ggccatgctc gccttcaagg tcgggaacct caccaccgcg gatgtcgtag acggctatcg
     3601 ggtcaaggcg aacttcgaca atgtcggcgg tctgaaggta aaggcggccg tgacggttgc
     3661 cggagtgcgc gtcggaaggg tcacgggcat cgcgttcgac agcaacaagt atcaggccgt
     3721 cgtcacgatg gatatcggcg gacaatacaa gaatattccg gtggacagca gcgcgaccat
     3781 cctgacctcg ggtttgctgg gcgatcagta cattggcctc gagcccggcg gcgccgagga
     3841 atatctcaag gatggagatg ccattcgcac gacgcagtcg gccctggttc tcgaaaagct
     3901 ggtcggccag gtgattttca acaagggcaa tgaaggtggc gcggccaagt aacgcgccat
     3961 acaaaaagga gtccttatga aaagattcgt tattgtcatg ctggttctgt tgcttgcgcc
     4021 gcttgcgcca gcgccggccg cggatgtccc gccggatcag ctggcgcgcg agacgaccga
     4081 caagatcgtg gtgctgctca aggccaacaa agacgcctac agcaaggacc acaagaagct
     4141 ttacgtcatg gtggacgagc atgtgctggc gcatttcgat tttcgcgcca tgtcacgcac
     4201 ggtgctggga cgtcactggc gcgcggccaa cgaggatcag cgcacgaaat tcaccacgga
     4261 attccgcgag ctgctggtgc gcacctacgc cacggcactg ctcaagtaca acgacgagca
     4321 gattgtctat ctgccgttcc gcatgagtcc ggaggatcgt accgccacgg taaaatcgga
     4381 agtccgccgc aaggacggca gcccgccgat agccatccag tacagtttct accacactga
     4441 caagggctgg aaggtgtacg acgtggcgat cgagggcgcg agtctcgtct cgacatatca
     4501 atccactttt tccgcgcgcg tgcagaagga aggtctggac gccctgatag cgggccttgc
     4561 tcaggacaac aaggccgccg ccaccggcaa gaccgcttcc aacggcggca agacgagcgt
     4621 cacccggtga gcgagaacgg ttttgcgtcc cgcggcggcg ggacattcga aatgtccggg
     4681 cggttgacgt ttcagaccgt gccgcaattc caggcacatg ccggcagtct gtttcagggg
     4741 gatacccagc cggtgacgat cgacatgcgg ggcgtcacac tggccgacag cgccgggttg
     4801 gcgctcatga tcgaatggct gcagctggcg cgcgccgcca aacgtcagct cgtcttcact
     4861 aatattcccg agcaagtgca tcatcttatc cgcgtcaacg gcctcacgca ggttttcgcg
     4921 ttgaaataac attctggagc cgcgccggcg gtttcataaa tagcgccaac cgtgtcagcg
     4981 atttcgattt ccgggctcca taagcgttac ccccgcgttc atgccctcga tggcatagat
     5041 ctcacggtcg agcggggaga attcttcggc ctgctcggcc cgaacggcgc cggcaaatcc
     5101 accctgatca atatcgtagc gggactgacc cgtttcgatg ccggtcgcgt gagcgtgctg
     5161 ggccaagacg tggtgacggc ctaccggcag acccgccggc tcattggcgt ggtgccgcag
     5221 gagctgacct acgatccctt tttcaacgtg cgcgaggtgc tgcgcatcca gtcgggctat
     5281 ttcggcctgc ataaaaatga tgcctggatc gacgagttgt tggcagcgct catgctgacc
     5341 gaaaaggccg gcgccaacat gcgcgccctt tccggcggca tgaagcggcg tgtgatgatc
     5401 gcgcaggcgc tggtgcaccg gcccgaggtg gtggtgctgg acgagcccac cgcgggtgtg
     5461 gacgtggaat tgcgcaagtc cctgtggacc tttatccgcc gcctgcacag tgagggccac
     5521 accattgtgt tgaccacgca ctacctggaa gaagccgagg ccttgtgcca tcgcatcgcc
     5581 atcctgaatc gtggcaagct tgtcgcgctc gataccaagc aggcgctgct ggcgcgtggc
     5641 atcggccgca cgcggcgggt ctgcatcaac accgaacggc cgctggcaag cctgcccggg
     5701 gaactggagc aaaaagtgcg acgccgggac ggcaatatga tcgagctgca actcgacaag
     5761 ggtactgact ccgtcatgga cacgctcgac accttgcgcg cacacggtgc cgtgatcacc
     5821 gatctcaaca tggagggggc tgatttggag gatgtgttcg tcgagctgac gcggagagac
     5881 agcgcgtgaa tctgatcggg ttctataccc ttttttacaa ggaggtgctg cgcttcggca
     5941 aggtgctgtt gcagactttg attgcaccgg tcatcacggc cttgttgtac ctgctggttt
     6001 tcgggcaggt gttcgagggc cgggtgcagg ctttctctgg cgtgagttac acgatgttcc
     6061 tgatccccgg cctcgtcatg atgtcggtga tacagaatgc cttcgccaac agctcctcga
     6121 gccttatcca gtccaaggtg acgggcaaca tcgtattcat gctgctgaca ccactgtcgc
     6181 atctggaatt ctttctggcg ttcgtgctgg cgtcggtggt gcgcggaata ctggtgggca
     6241 cgggggtata tctcgtggcg ctggttttca cgacggtgcc tgtgaaacac ccgggcttca
     6301 tcctgctctt catgttcata agcagcgcca ccctgggcgc catcggggtc atcgccggta
     6361 tctgggccga gaaattcgat cagctggcgg ggttccagaa tttcatcatc ctgcctttgt
     6421 cgttcctctc cggcgtgttc tactcgatcc attcattgcc ggagttctgg cagaagctgt
     6481 cgcacttcaa tccgtttttc tacatgatcg acggtttccg ttacggcttt ttcggggttt
     6541 ccgatgtcgc accctcgcac agtctcgcga tcgtgacgct gtttctggtg gttttgtccg
     6601 gcatttcact tgtgttgctc aggaccggtt acaagctgcg cgattgagga gttattggtc
     6661 ggggccttcg ttccagttca tcgggcacgg gatatccact gcccggtctg tttcaaccgg
     6721 cgcgcggcgc cggttttcta tcccttaagg gtatatctct tgaaatggcc cgaagaggac
     6781 ggataataaa acccatcatg atgcagcctg atgacatcaa gcagatgatc gagcgcggtt
     6841 tgccgggcgc gcgcgtgtcg gtgaccggcg acggccatca tttcgaggcc gaggtgatag
     6901 ccagcgaatt cgccggcaag agtactctgc aacagcacca gatcgtgtac cgcacgctcg
     6961 gcaacaaggt gggtaacgaa atccacgcgc tttccctgcg cacccaggcg cccggcgagt
     7021 gacgcagacc cgaccggcaa tcttccttta ctgaagcaac aagaggacgg catgaacgta
     7081 caagacaaaa tccgcgaaca gctttccggc gacaaaattg tgctctacat gaagggcacg
     7141 ccccagtttc cgcagtgcgg tttttccgcc aaggcggcgc aaatgctggg cgcctgcggg
     7201 gcgaaatacg ccagctacga catattgatg gacccggaga tccgccaggg cctcaaggaa
     7261 tactcgaact ggccgacttt cccccagctc tacgtcaacg gcgaactggt cggaggctgt
     7321 gatatcatga ccgagctgtt ccagaagggc gagcttcaga aattggttgc caaggcgatg
     7381 ggttgagctt cggccgtatg gaccgaacaa agccgccttc tggcggcttt tgttttttgt
     7441 agcaaagaga attttcatac atggacaaac tgattgttac gggtggtagc gcgctgcgcg
     7501 gcgagataaa gatctcgggc gcgaagaatg ccgcgctgcc ggtgctggtg gcctcgctgc
     7561 taaccaacga gacggtgagc atcggcaacg tgccgcacct gcaggacatc accacgacca
     7621 tggaactgct cggtcgcatg ggcgtgcgct tggtggtcgg cgacaagatg gtgatcgagg
     7681 ccgactcgag ccagaacacc tcggtgcgtg cgccgtacga gctggtgaaa accatgcgtg
     7741 cctcgatcct ggtgctgggt ccgctgctcg cgcgtttcgg cgaggccgag gtgtccctgc
     7801 ccggcggttg cgccatcggc tcgcgtccgg tgaacctgca catcaagggc ttgcaggcca
     7861 tgggtgcgga gatcaatctc gacggcggtt atatccgcgc caaggcgaag cgcctcaagg
     7921 gtgcgcgcat cttcatggat ctcgtttccg tcacgggcac ggagaacatc atgatggcgg
     7981 caacgctggc ggatgggatg acgatcatcg aaaacgccgc gcgcgaaccc gaagtggcgg
     8041 atcttgcccg gtgcctcaac tccatgggcg cgcgcatcac gggcgcgggc acggacgaga
     8101 tcgcggtgga aggcgtggag cgtttgcacg gcgccgtgca tgaagtcatt cctgaccgca
     8161 tcgaaaccgg gacttatctg gttgccggcg cgatgaccgg gggcagcgtg cgcctgcgca
     8221 acgcgcgccc cgacctgttg caggcggtgc tcgacaagct gcgcgagacc ggcgccgtca
     8281 tcg
//
LOCUS       DBNGZE010000140         5817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_922712, whole genome shotgun sequence.
ACCESSION   DBNGZE010000140 DBNGZE010000000
VERSION     DBNGZE010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_922712"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..2505
                     /locus_tag="AC16FV_03530"
     CDS             295..2505
                     /locus_tag="AC16FV_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2849168.1"
                     /translation="MLREKASKYALYGVYIASVSVVIATLLVSYVMVDAITIEGIILS
                     QTSNIALWTVDAMPFVFAMWGQYASYRMAREADEFVQSKTQTLREALTKADFTSKAKS
                     DFFAKMSHELRTPINGIIGMSDLVLETKLTREQRRHIDIIKSSASGLLTLINDLLDFS
                     KIEAGKLELEEIEFDLRDCVEKSATLLAQQAYAKGLALTNLIQNDVPGRLLGDPGLLR
                     QIIINLLGNAIKFTHTGEVVLSVKKLPDESKGRIRLHVEVADTGIGLSAKDQARLFQP
                     YNQAGASTSRRYGGTGLGLTISKELVEAMGGQIGVKSLEGKGSVFWFTADFRKPAETS
                     DTLSPTRIDLKGLRVLVADGNIPARVGLVDQLKALGLEVQSIGNGNAALQMIRSAAGS
                     DFRFDLVLVDMFLPNLSGEELGREIKSRPETRDTVLIILTSAGVRGDAQRLNQLGFAG
                     YFGKPIPPEDLQDALKAVMATRGMDEEMRQRAGLVTKYTLSDMRKKFSRLLLVEDSEV
                     NREIMQKMLSKQGYSADIAENGRAAIAAATRGDYGLILMDLHLPDISGIEAIEAIRAL
                     PGARARVAVAVLTAGASDEEMARCRALEVNDFLTKPLDSRVLVDTLKRYRIAPISVTE
                     DAPPAHTPYVVSPAEKDAVPDAKLVDIFVKESTQRLESLREAVTSKDAQRAAREAHTL
                     KSSSAYFQADAMHEAAARLEEMADRGQLGKAKPALEKLEEAYARLHATLTKNKN"
     gene            complement(2502..3650)
                     /locus_tag="AC16FV_03535"
     CDS             complement(2502..3650)
                     /locus_tag="AC16FV_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2849169.1"
                     /translation="MTHRAKRLSRIEPFHVMDILARARAMETQGRSVIHMEIGEPDFP
                     TAPAIVQAGVAALQSGHTHYTTALGLPELRAAIAASYPADARPDAGQVVVTPGASGAL
                     QLIFAALINPGDQVLMADPGYPCNRHFVSLFEGEPLAIPVDASTGYQLNADLVRRHWT
                     RCTVAVLLASPSNPTGTLVPPEEMARIARAVEELGGVLIVDEIYHGLVYGEEAVTALV
                     HSQNLFVVNSFSKYYGMTGWRLGWLVAPAPYVPEIDKLAQNIFLAASTPAQYAALAAF
                     DAGVQQELQKRRDIFRERRDFLLPALRELGFDIPLIPQGAFYLYADCSRFTDDSQAFA
                     IKLLDETGVAITPGLDFGAHRAARHVRFSYANTLENLREGVSRLRSYL"
     gene            complement(3647..4411)
                     /locus_tag="AC16FV_03540"
     CDS             complement(3647..4411)
                     /locus_tag="AC16FV_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2849170.1"
                     /translation="MTLKALIFDVDGTLADTERDGHRLAFNRAFAEAGLDWEWDVGLY
                     GELLAVTGGKERMRHYVERYRPDYRKPTDFDVLVARLHAAKTRHYTELLGQGGIPLRP
                     GVRRLLDEARQAGIHLAIATTTTPANVSALLQHAMAPDAESWFEVIGAGDVVPAKKPA
                     PDIYYYVLEKLGLAPAQCLAFEDSENGLRSARAAGLKTVVTVNDYTRAHDFNGAAIVL
                     DHLGEPDQAFQVFSGPPVNGSQMLDVPLIRQLMFVS"
     gene            4573..5022
                     /gene="dksA"
                     /locus_tag="AC16FV_03545"
     CDS             4573..5022
                     /gene="dksA"
                     /locus_tag="AC16FV_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR2849171.1"
                     /translation="MTARKKKEKEQSISPVSGIKPYVAKKGEKYMNKKQQEHFRAVLN
                     AWKNELEEEITRTVQQMRDVPENPPDPNDRASQETDMSLELRSRDRERKLIKKIDEAI
                     ERIDTGDYGYCEVCGVEIGVERLEARPTAELCIDCKTLDEIREKQMG"
     gene            5165..>5817
                     /locus_tag="AC16FV_03550"
     CDS             5165..>5817
                     /locus_tag="AC16FV_03550"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR2849172.1"
                     /translation="MELPLLIKALILGIVEGLTEFLPVSSTGHLIIVGDLLNFNDDKG
                     KVFEIVIQLGAILGVVWDYRTRVGRLITGLNTKHEQRFLLNLFIAFLPAAIVGLLLHQ
                     QIKTYLFNPITVAIALIVGGFVILWIERLHLRVRVTSMEDLGPVDALKIGIAQTFSLF
                     PGVSRAGATIMGGLLTGLSRTAATEFSFLLAMPVMFAATFFELIKNHTLFEAADVPV"
BASE COUNT         1282 a         1764 c         1676 g         1095 t
ORIGIN      
        1 ttaaggtgcc cgctgcaagc ccgccgcagc gttggcacag cgaacatgga caaggaccca
       61 gggtgtgtgt gtaagcgcgc aaccaacacc ctggcgacat cttccaggtc cgggcccaag
      121 accagtattt cccgtctaat tcccctgccc ccgactgccg ctacccaagc cgttcacgcc
      181 cagctacgac gattcccgtc accaaaatca atctacactt atttaaagac caggcagacg
      241 gggcggcgtc aaaatctcat ggaccagagg caacttcgcg cagaggatct cctgttgctt
      301 cgggagaagg cctccaaata cgctttgtat ggcgtgtata tcgccagcgt gagcgtcgtt
      361 atcgcgacac tgctggtgag ctacgtcatg gtcgatgcca tcaccataga aggcatcatt
      421 ctctcccaaa cctccaacat cgcgctgtgg accgtggatg ccatgccctt cgtgttcgcc
      481 atgtgggggc aatatgcgtc ctatcgcatg gcgcgcgagg ccgacgagtt tgtgcaaagc
      541 aagacccaaa ccttgcgcga ggcgctcacc aaggccgact ttaccagcaa ggccaagagc
      601 gacttcttcg ccaaaatgag ccacgagctg cgcacgccca tcaacggcat catcgggatg
      661 agcgacctgg tgctcgagac caaactgacc agggaacaac gccgtcatat cgatatcatc
      721 aaatcctcgg ccagcggctt gctcaccctg atcaacgatc tgctggattt ttccaagata
      781 gaggcgggta agcttgagct ggaagaaatc gaattcgacc tgcgcgattg cgtcgaaaaa
      841 tccgccaccc tgctcgcgca gcaggcgtac gccaaggggc tcgcgctcac caacctgatc
      901 cagaacgacg tgcccggtcg cttgctgggc gatcccggac tcctgcggca gatcatcatc
      961 aatctgctcg gcaacgccat caaattcacc cataccggcg aggtggttct gagcgtcaag
     1021 aagctccccg atgagtccaa gggccggata cggttgcatg tggaagtggc cgacaccggc
     1081 atcggacttt cggccaagga tcaggccagg ctgtttcaac cctacaatca ggccggagcc
     1141 tcgacgtcgc gtcgctatgg cggcaccggc ctcggcctga ccatcagcaa ggaactggtg
     1201 gaggccatgg gtggacagat cggcgtgaag agcctggaag gtaaaggcag tgttttctgg
     1261 ttcaccgccg atttccgcaa acccgccgag accagcgaca ccctctcgcc cacccggatt
     1321 gatctcaagg gcctgcgcgt gctggtggcc gacggtaata tcccggcgcg cgtcggtctg
     1381 gtcgaccaat tgaaggcgct gggcctggaa gtgcagtcca tcggcaacgg caatgcggcg
     1441 ctgcaaatga ttcgttccgc cgcgggttcg gacttccgat tcgatctggt gctggtggac
     1501 atgtttctgc ccaacctgag cggcgaagag ctggggcgcg aaatcaagtc acgacccgag
     1561 acgcgcgaca ccgtcctgat tatcctgact tcggcgggcg tgcgcggcga tgcgcaacgt
     1621 ctgaaccagc tgggttttgc cggttatttc ggcaagccta ttcctcccga agacctgcaa
     1681 gacgcgctga aagccgtcat ggcaacgcgg ggcatggatg aagaaatgcg tcagcgtgcc
     1741 ggtctggtca cgaaatacac gctgtccgac atgcgcaaaa aattctcgcg cctcttgctc
     1801 gtggaggaca gcgaggtcaa tcgcgagatc atgcagaaaa tgctgtccaa gcagggttac
     1861 tcggccgata tcgcggaaaa cggacgcgcg gcgatcgcgg cggcgacacg cggggactac
     1921 ggcctgattc tcatggacct gcatctcccg gacatcagcg ggattgaagc cattgaagcg
     1981 attcgcgccc tgcccggcgc gcgcgcaagg gtagcggtcg ccgtgctcac ggcgggcgca
     2041 tcggatgagg aaatggcgcg ctgtcgcgcg ctggaagtca atgacttcct gaccaaacct
     2101 ctggacagca gagtgctcgt cgatacgctg aagcgctatc ggatagcgcc gatatccgtg
     2161 acggaggatg caccgcctgc gcacaccccg tacgtcgtgt caccggcgga gaaggacgcc
     2221 gtgccggatg cgaaactggt tgatatcttc gtcaaggagt ccacgcagag actcgaatcg
     2281 ctgcgtgagg cggtgacttc caaggacgcc cagcgtgccg cgcgagaggc gcacacgctc
     2341 aaaagctcga gcgcctattt ccaggccgat gccatgcacg aagcggctgc acgactcgag
     2401 gaaatggcgg accgcggaca actcggcaag gcaaagcccg cgctcgagaa gctcgaagaa
     2461 gcgtatgcgc gcctgcatgc cactctgaca aaaaacaaga attaaagata actccgcaag
     2521 cgcgacacgc cttcgcgcag attctcgagc gtgttggcat aggagaagcg cacatgccgc
     2581 gccgcccgat gagctccgaa atcgaggccc ggcgtgatgg ccaccccggt ttcatcgagc
     2641 agcttgatgg cgaaggcctg gctgtcatcg gtaaaacgcg aacaatccgc atacaaatag
     2701 aaagcaccct gcgggatcag cgggatatcg aagcccagtt cgcgcaaggc tgggaggaga
     2761 aaatcgcgtc tttcacggaa aatatcccga cgcttctgca attcctgttg caccccggca
     2821 tcaaaagcag ccagcgcggc gtattgcgcc ggcgtgctgg cggcaagaaa aatattctga
     2881 gccagtttgt cgatctccgg tacataagga gccggcgcca ccagccaccc cagccgccag
     2941 ccggtcatgc cgtaatattt ggagaagctg ttcaccacga aaaggttctg cgagtgcacc
     3001 agggccgtca cggcctcttc gccgtataca aggccgtgat agatctcatc cacgatcaat
     3061 actccgccca gctcttccac ggcccgggca atccgcgcca tctcttccgg tggaaccaga
     3121 gtgcccgtgg gattggaagg cgaggccaac agcacggcca cggtgcacct ggtccagtgc
     3181 cggcgaacca gatcggcatt tagctgatac ccggtcgagg catccaccgg tatggcaagt
     3241 ggctcacctt caaacaaact gacaaagtgg cgattgcagg gataacccgg atcggccata
     3301 agcacctgat cgccgggatt gatcagcgcc gcgaaaatca gttgcagggc gcccgaggcg
     3361 ccgggcgtga ccaccacttg gccggcatcc ggacgtgcat cagctggata gcttgcggca
     3421 atggccgcgc gcagttccgg caggcccaac gcggtggtgt aatgcgtgtg gccggactgg
     3481 agcgccgcta cgccggcctg aacgatggcc ggtgccgtgg gaaaatccgg ctcgccgatt
     3541 tccatgtgga tcacggaccg gccctgcgtc tccatggcgc gggcgcgggc aagaatatcc
     3601 atgacgtgaa atggctcgat tctcgacagg cgcttggcgc ggtgagtcat gaaacgaaca
     3661 ttaattgcct gatcaatggg acatcaagca tctgcgagcc attcaccgga ggtccggaaa
     3721 acacctgaaa cgcctgatcc ggttcaccaa gatgatccag cacgatcgct gcaccgttga
     3781 aatcgtgcgc acgcgtatag tcattgacgg tgacgacggt tttcagtccc gctgcgcgcg
     3841 cggagcgcag gccgttctcg gagtcctcga aggccagaca ttgtgcgggc gcgagcccaa
     3901 gcttttcaag aacgtaataa tagatatccg gagccggctt cttggccggc accacatcgc
     3961 cagcgccgat cacctcaaac caggattcgg catcgggtgc catggcatgt tgcaataagg
     4021 cactgacatt ggctggcgtg gtagtcgtgg cgatcgccag gtgaatccct gcctgacgcg
     4081 cctcgtcgag cagacgccgc acacccggtc gcagcggaat gccgccttgc cccagcagct
     4141 cggtgtaatg gcgcgtcttg gccgcgtgca accgcgcgac cagcacgtcg aagtcggtgg
     4201 gcttgcggta atccgggcgg taacgctcca cgtaatgacg catgcgttcc ttgccgccgg
     4261 tcaccgccag caattcaccg tacaatccta cgtcccactc ccagtcaagc cccgcctcgg
     4321 cgaacgcccg gttgaaggcc agccggtgcc catcgcgctc ggtgtcggcc agtgtcccgt
     4381 caacatcaaa aatcaacgcc ttgagtgtca tggaaatgtc cacaaaatcc ggtcaaattc
     4441 cgttgttacg gcaatacggg ttggcggcac ctatactctt ttgttgcggc tgcggaagct
     4501 ttctgatata gattcgcgct tattttttta ccccctaccc ttcaggggcc acattatcgc
     4561 aggagttgaa gcatgacggc cagaaaaaag aaggaaaagg aacagtccat ttcgcctgtt
     4621 tcgggcatca agccctacgt ggcgaaaaag ggcgagaagt acatgaacaa gaaacagcag
     4681 gaacattttc gtgctgtgtt gaacgcgtgg aaaaacgaac tggaagagga gatcacccgc
     4741 accgtgcagc agatgcgcga tgtgccggaa aatccgcccg atccgaacga ccgcgcgagc
     4801 caggaaaccg acatgtctct cgaactgcgt tcacgcgacc gcgaacgcaa gctcatcaag
     4861 aaaatcgacg aggccatcga acgcattgac accggcgatt acggctactg cgaggtctgc
     4921 ggcgtcgaga tcggcgtgga acgcctcgag gcccggccca ccgcggaact ttgcatcgac
     4981 tgcaaaacac tcgacgaaat ccgcgaaaaa cagatgggct gaagccgtct tttcatcgaa
     5041 atctccaccg tcatttgtgt gaccacgcct gtcgatagac aggcgacgcg tgttgataga
     5101 caagtccgcc gtcgcccgcg cgaacgcctg ctatcattca cataatcagt taaccggctg
     5161 agccatggaa cttccactgt tgatcaaggc gttaatcctc ggcatcgtcg agggtctgac
     5221 cgaatttctg ccggtatcct ccaccggaca tctgatcatc gtgggcgacc tgctcaattt
     5281 caacgatgac aagggcaagg tattcgagat tgtcatccag ctcggcgcca ttctcggcgt
     5341 ggtctgggat taccgcacgc gtgtcggccg cctgatcacc gggttgaaca cgaaacatga
     5401 acaacgtttt ctgctgaacc tgtttattgc cttcctgccc gccgccatcg tgggcctgtt
     5461 gttgcatcag cagatcaaga cgtatctttt caatccgatc accgtggcga ttgcgctcat
     5521 cgtcggcggt ttcgtgatcc tctggatcga gcggctgcat ctacgcgtac gcgtcaccag
     5581 catggaagat cttgggcccg tggatgccct gaaaatcggc atcgcccaga cgttttcact
     5641 tttccccggc gtgtcacgcg ccggcgccac catcatgggc ggcctgctca ccggcctgtc
     5701 gcgcaccgcg gccaccgagt tttcctttct attggcgatg ccggtcatgt tcgccgcaac
     5761 gttctttgag ctgataaaaa accataccct gtttgaagcc gccgatgttc cggtctt
//
LOCUS       DBNGZE010000141         4268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1478543, whole genome shotgun sequence.
ACCESSION   DBNGZE010000141 DBNGZE010000000
VERSION     DBNGZE010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1478543"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="AC16FV_03555"
     CDS             <1..154
                     /locus_tag="AC16FV_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR2849173.1"
                     /translation="HGFTVQTAQLGWRPKNPVTLGDSERAEVEAFLEAVDADDDVQNV
                     YAGLTG"
     gene            525..1037
                     /locus_tag="AC16FV_03560"
     CDS             525..1037
                     /locus_tag="AC16FV_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849174.1"
                     /translation="MLNAEAAGAKKTPTDKQLIASAMSAAPAKVAKGATIVAMEADGK
                     MRTIRQGTNGFTCMPDNPATPGPDPMCMDKNAMEWVHAWIGHKNPTPGKIGFMYMLAG
                     GTDASNTDPYATKPEPNNNWIKTGPHVMVVGADASFYDMYPKDAKPDTSVPYVMFPGT
                     PYQHLMAPVK"
     gene            1127..1747
                     /locus_tag="AC16FV_03565"
     CDS             1127..1747
                     /locus_tag="AC16FV_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849175.1"
                     /translation="MKSHLFAAVAFMIVVAASAEAALVERDLFTPGDHYLTWDTATGL
                     EWLDLTLTIGISYNNVAAGYGGYTTTYGFVFANQADTTTLFADAGLSPVSGTSSDPAK
                     VAAGTSLIALMGCTANCATTPASRGFADLNNFSVTVATAPALSINPGVSVSWDADGAG
                     ILGKTQSIAGDGSWLVRTAAVPVPASVWLLGSGLLWLLGMACRKAA"
     gene            1770..2207
                     /locus_tag="AC16FV_03570"
     CDS             1770..2207
                     /locus_tag="AC16FV_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2849176.1"
                     /translation="MLKRILLPLLILPFFVACGRQEPPLPSVPPAPQTTAPTPVPVPQ
                     ATPPAAAPAAPMVAGGNLAKGEATYKQTCFACHAAGVAGAPKLGDKAAWAPRIAQGMD
                     TLHTHSLKGFQGKVGMMPPKGGNTALSDADVMAAVDYMVSQSK"
     gene            complement(2223..3233)
                     /locus_tag="AC16FV_03575"
     CDS             complement(2223..3233)
                     /locus_tag="AC16FV_03575"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR2849177.1"
                     /translation="MKICIVGAGAIGGLMGAKLALAGEEVTLIARGAHLDAMQKNGLK
                     LIEEDGSEHVAKNVKATSRIAEAGKQDVVILALKAHQVEPIAKEIPALFHDQTVVVTT
                     QNGIPWWYFQKHGGEFEGRRVETVDPQGHLMASIEVERILGCVVYPAAEIVSPGVIKL
                     IEGNRFPVGELDGTETERVKNITATFVKAGFKSPVLKDIRAEIWLKLWGNLSFNPISA
                     LTHSTLVDICQFPLTRQLAADMMTEAQTIAHKLNIEFRVSLEKRIAGAEKVGKHKTSM
                     LQDIEIGRAIEIDALVGAVIELGKLTNTPTPHIDSVYACVKLLAKTLGEEKGRLKIEK
                     IS"
     gene            complement(3250..4014)
                     /locus_tag="AC16FV_03580"
     CDS             complement(3250..4014)
                     /locus_tag="AC16FV_03580"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR2849178.1"
                     /translation="MHWVRFERGGNIGFGTLHDGVIRIHEGDMFTDSHATNVTVPLSE
                     VTLLTPCEPGKMIALWNNFHALAAKLNLTPPPEPLYLLKAPNSFLASGQTIRVPASYT
                     GKVVYEGELGVVIGKHASHVSEADAAKYIFGYTCSNDVTAAELIQKDPTFAQWTRAKG
                     FDTFGVFGPVIATEVNPLDLHVKTLLDGKERQNYPVSDMIFKPAQIVSLISRDMTLYP
                     GDVISCGTSVGVGSMKPGSKVEVVIDGIGTLSNEYK"
BASE COUNT          835 a         1255 c         1278 g          900 t
ORIGIN      
        1 gcacggattc accgtgcaaa ccgcgcagtt gggttggcgg ccgaagaatc cggtgacact
       61 gggtgactcc gagcgcgcgg aggtcgaggc ttttctcgaa gcggtcgatg ccgatgatga
      121 tgtgcaaaat gtgtatgcgg ggctcacagg ctgaagccgg tattgtcttg gcttgtcttt
      181 tacctcccct gagcagttgt tgcccgttcc gcatgttgtt tctgaataaa taaagcgttg
      241 cctggtccaa ttcgccaata agcatgaggc ttttcgcagg attttgattt tttctgaggt
      301 tgataaaaat ctgaaattga tgaaataaag agaaaggcgg agaagataaa aaagcacatg
      361 tgccgttttt tttgaccacg aaggcatatg aataataacc agcagacctc gaggagatct
      421 caatgcaaac gccaaaaatc agtagcccta ctcgctccgc tcgttcgtcc tgtcataata
      481 aaattgtgtg cagtctgatg atgttcgcat catcttttct gttgatgctg aatgccgaag
      541 cggcaggcgc gaagaaaacc cccaccgaca aacagctgat cgcaagcgcc atgtccgcgg
      601 cgccggccaa ggttgcaaaa ggagcgacca tcgtggccat ggaggcggat ggcaaaatgc
      661 gcacgatacg ccagggaacg aacggcttca cctgcatgcc ggacaacccg gcgacacccg
      721 gtcccgaccc catgtgcatg gacaagaacg ccatggagtg ggtgcatgcg tggatcgggc
      781 acaagaatcc aacgccgggg aaaatcggtt tcatgtacat gctggcgggc ggaacggacg
      841 ccagcaacac cgatccctat gcaaccaaac ccgagcccaa caataactgg atcaagaccg
      901 ggccgcacgt gatggttgtc ggcgccgacg cgagcttcta cgacatgtat ccgaaggacg
      961 cgaaacccga cacctcggtc ccctacgtga tgttccccgg cacgccgtac cagcacctga
     1021 tggcgccggt gaaataggcg aagaaatacg agaaaggagg acgataggga tcagatcatt
     1081 cctttcgtcc tcttttgtat tttcctatcc ggcaggaaga gggcccatga aatcacatct
     1141 gttcgccgcc gtcgcgttca tgatcgtggt tgccgcatct gcggaagcgg cgttggtcga
     1201 aagggattta tttacgcccg gagatcatta tctgacatgg gatacggcca ccggacttga
     1261 atggctggac ctgacgctca ctatcggcat ttcctacaat aacgtcgcgg cgggttacgg
     1321 cggttacacc accacttacg gattcgtttt tgccaaccag gcggacacga ccacgttgtt
     1381 cgccgacgcc ggcctctcgc ccgtgagcgg caccagctcc gacccggcca aagtagcggc
     1441 cggcacttcg ctgattgcgc tcatgggttg taccgcgaat tgcgccacca cgcccgccag
     1501 ccgcggtttt gcggatctca acaatttttc agtcactgtg gccacggcgc cggcactctc
     1561 aatcaatccg ggtgtcagcg ttagttggga tgcagacggc gccggcattc tgggcaaaac
     1621 ccaatcgatc gcgggtgacg gttcgtggct ggtccgaacc gctgccgtgc cggtgcccgc
     1681 ttccgtgtgg ctgctcggca gcggcctgtt gtggttgctt ggcatggctt gccgcaaagc
     1741 cgcctgagtc agaaaaagga ggagtcccca tgctgaaacg aatattgctt ccgctgttga
     1801 tccttccgtt cttcgttgcc tgtggcagac aggaaccgcc tcttccttcg gtgccgcccg
     1861 caccgcaaac gactgcgccg actccggtcc cggttccaca agcgacgccg ccagccgctg
     1921 cgcccgcggc tcccatggtt gccggcggca atctcgccaa gggtgaagcc acatacaagc
     1981 aaacctgttt cgcctgtcac gccgcgggcg tggctggtgc gcccaagctg ggcgacaagg
     2041 ctgcgtgggc cccgcgcatc gcccaaggca tggacacgtt gcacacgcat tctctcaagg
     2101 gctttcaggg caaggtcggc atgatgccgc ccaagggtgg gaacaccgct ttgagcgacg
     2161 cggacgtaat ggcagcggtc gattacatgg tgtcacagtc gaagtgatcc ggaaattcta
     2221 tttcagctga ttttttctat tttcagcctg cctttttcct cacccagggt cttggccagc
     2281 agcttgacgc aggcatagac tgaatcaatg tgcggcgtgg gcgtgttggt gagcttgccc
     2341 agttcgatca cggcgccgac cagggcgtcg atctcgatgg cgcggccgat ctcgatgtcc
     2401 tgcagcatgg aggtcttgtg cttgccgacc ttctcggcgc cggcgatgcg cttctccagt
     2461 gacacgcgga attcgatgtt gagtttatgc gcgatggtct gcgcctcggt catcatgtcg
     2521 gcggcgagct ggcgcgtcag cgggaactgg cagatgtcca ccagcgtgga atgggtcagg
     2581 gcgctgatcg ggttgaagct gaggttgccc cagagcttga gccagatctc cgcgcggatg
     2641 tccttcagca ccggcgactt gaagccggcc ttgacaaacg tcgccgtgat gtttttcacg
     2701 cgttcggttt cggtgccgtc gagctcgcct accggaaaac ggttgccctc gatcagcttg
     2761 atcacgcccg gcgagacgat ttcggcggcg ggatagacca cgcagccgag gatgcgctcg
     2821 acctcgatgc tggccatgag atggccctgc gggtccacgg tctcgacgcg ccggccctcg
     2881 aactcgccgc cgtgcttctg gaaataccac cagggaatgc cgttctgggt cgtgaccacc
     2941 acggtctggt cgtggaacag ggccgggatc tccttcgcga tcggttcgac ttggtgcgct
     3001 ttcagtgcca ggatcaccac gtcctgcttg ccggcctcgg cgatcctgct ggtcgccttg
     3061 acgttcttcg ccacgtgctc gctgccgtcc tcctcgatca gcttcaggcc gtttttttgc
     3121 atggcatcga ggtgcgcgcc gcgcgcgatc agcgtgactt cctcgcccgc gagcgcaagc
     3181 ttcgcgccca tcaaaccgcc gatggcgccg gcgccgacga tgcatatctt catgggtgat
     3241 tcctccggat catttgtatt cgttggacag cgtgccgata ccgtcgatga cgacctcgac
     3301 cttgctgcct ggtttcatcg aaccgacgcc gacggacgtg ccgcaggaaa tgacgtcgcc
     3361 cgggtacagc gtcatgtcgc gcgagatcag gctcacgatc tgcgccggct tgaaaatcat
     3421 gtcgcttacc gggtaattct gccgctcctt gccgtcgagc agggtcttta cgtgcaggtc
     3481 gagcggattc acttccgtgg cgatcaccgg gccgaacacg ccgaaggtgt cgaagccctt
     3541 ggcgcgcgtc cactgggcaa aagtcgggtc tttctggata agttcggcgg cggtgacatc
     3601 gttggagcag gtgtaaccga atatgtactt ggcggcatcg gcttcggaaa cgtgcgaagc
     3661 gtgcttgccg atcacgaccc caagctcccc ttcgtacacg accttgccgg tataagacgc
     3721 cggcacgcgg atggtctggc cgctggcgag gaaggaattc ggcgccttga gcaggtacag
     3781 cggttcgggt ggcggcgtga ggttgagttt ggccgccagc gcgtgaaaat tgttccacaa
     3841 cgcgatcatc ttgccgggct cgcagggcgt gagcagcgtg acctcggaga gcggcacggt
     3901 cacgttcgtg gcgtggctgt cggtgaacat gtcgccttcg tggatacgga taacgccgtc
     3961 atgcagtgtt ccgaatccga tatttccgcc gcgctcgaaa cgaacccagt gcataaaacc
     4021 tcctccgccg tgttatcgtg acgatccatt atcgccgcgg atcgcgagac gtatgctacc
     4081 ttctggcgcc gcgtgtcgcc agccgcatct tgtttccgca agatgaaatt cgtatcgtgg
     4141 ccgcgccgtt gtaaaaaccg gtttcactag acgaaaaaaa tgcgcgacaa ccaatacccc
     4201 ggacgttcgg tcgtgatgag cacgcgcggc atgatcgcca ccagccagcc gatggcgacg
     4261 caggcggg
//
LOCUS       DBNGZE010000142         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_367662, whole genome shotgun sequence.
ACCESSION   DBNGZE010000142 DBNGZE010000000
VERSION     DBNGZE010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_367662"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC16FV_03585"
     CDS             complement(<1..620)
                     /locus_tag="AC16FV_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849179.1"
                     /translation="MRTQIVLSVAVLLLVGCASSAEREAEQEKLNRLAETHVLLGSSY
                     IQRGQLDVAKEELDKALKLVPDHSQANNIMAVLQWRFKDYDKADRLFRKSLASDEKNA
                     SAQHNYGAFLCDHGKINEAVRHFDVAAANPLYPYAAEVNLNAGVCLMKRPSPGVAEKY
                     FREALRHGGQGEDDDSSDDHWSSSRVAGTERCGAVMMKGTFRETCTG"
     gene            complement(617..1717)
                     /gene="rlmN"
                     /locus_tag="AC16FV_03590"
     CDS             complement(617..1717)
                     /gene="rlmN"
                     /locus_tag="AC16FV_03590"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR2849180.1"
                     /translation="MSVAEKTNLLNLDRAAMQEFFTGIGEKPFRATQLIQWIHQYGVD
                     DFDAMTNLSKELRARLRDIAEIRVPEIIQDQIASDGTRKWLLRLDDGNAIETVFIPEE
                     DRGTLCVSSQVGCTLNCSFCSTGHQGFNRSLTVAEIISQLLVANRALGRDPKGDRIIT
                     NVVLMGMGEPLLNFDNVLPAMRLMLDDYAYGLSRRRVTLSTAGVVPMLERLREECPVS
                     LAVSLHAPNDALRDQLVPLNKKYPIEELLEACRRYCADSPRSRVTFEYVMLEGINDSI
                     EHAHELVRILSDVPSKVNLIPFNPFPQTQYRRSSKQAIDRFREVLIAAGLTTITRKTR
                     GDDIDAACGQLAGRVQDRTRRRERLAVRAGRT"
     gene            complement(1902..2099)
                     /gene="iscX"
                     /locus_tag="AC16FV_03595"
     CDS             complement(1902..2099)
                     /gene="iscX"
                     /locus_tag="AC16FV_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR2849181.1"
                     /translation="MKWTDTREIAIELSEKYPDVNPMSVRFTDLHKWVMGLEDFDDMP
                     EHSGEKILEAIQMAWMDEHGG"
     gene            complement(2119..2457)
                     /gene="fdx"
                     /locus_tag="AC16FV_03600"
     CDS             complement(2119..2457)
                     /gene="fdx"
                     /locus_tag="AC16FV_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018880475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HIR2849182.1"
                     /translation="MTRLTFLPHPQICPEGKTIEVEPGISICDAALNAGIPIEHACEK
                     ACACTTCHVVVREGFNTLNTAEEKEEDLLDKAWGLEPESRLSCQAKVGSQDLVIEIPK
                     YTINLVSEQH"
     gene            complement(2454..>2993)
                     /locus_tag="AC16FV_03605"
     CDS             complement(2454..>2993)
                     /locus_tag="AC16FV_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR2849183.1"
                     /translation="DCRSLARFELRGIPPMTAGAARIRVTFQIDADGLLGVSAREQTT
                     GVESSVTVKPSYGLTDNEIERMLRESIAHAREDDATRKLREEQVEADRLLEAVRSALG
                     VDGALLNTNERAEIENRLAELHVVRAGNDATSIRRAIKNLDQATQTFAQRRMDENIRK
                     ALAGHRLEEFDEPRKKSPS"
BASE COUNT          583 a          916 c          836 g          658 t
ORIGIN      
        1 cccgtacagg tttcccggaa ggtgcccttc atcatgactg ccccgcaccg ttccgtcccg
       61 gccacccggg aggaactcca atgatcatcg gaactgtcgt cgtcctcgcc ttggccgcca
      121 tggcgcaggg cctcgcggaa atatttttcg gcgacgcctg gagacggccg cttcatcagg
      181 caaacaccgg cgttcagatt gacttccgcc gcgtatggat acagcgggtt ggccgccgcc
      241 acatcgaagt gccgcacggc ttcatttatt ttcccgtgat cgcacagaaa agctccgtag
      301 ttgtgctgcg ccgaggcatt tttttcgtcg cttgccagag atttgcgaaa aagccggtcc
      361 gccttgtcat aatccttgaa gcgccattgc agcaccgcca tgatattgtt ggcctgcgaa
      421 tgatccggca ccagcttgag cgccttgtcc agctcttcct tggccacgtc cagctggcca
      481 cgctgtatgt aactggaacc gagcaataca tgtgtttccg ccagccggtt caatttttcc
      541 tgctcggctt cgcgctccgc ggatgaggcg caaccgacga gcagcagaac cgccactgac
      601 agaacaatct gtgtcctcat gtccggcccg ccctgaccgc caagcgctcg cgccggcgtg
      661 tacgatcctg tacgcgtccg gcgagctggc cgcaggccgc gtcgatgtca tcgccacgcg
      721 tcttgcgcgt gatcgtggtc agtcccgctg caatcaatac ctcgcgaaac cggtcgatgg
      781 cctgcttcga ggagcgccgg tactgcgtct gcggaaaggg attgaagggg atcagattca
      841 ccttggatgg cacgtcactc agaattctta ccagctcatg ggcatgttca atgctgtcat
      901 tgatcccttc cagcatcacg tactcgaatg tcacgcggct gcgtggcgaa tcagcgcagt
      961 agcgacggca ggcttcaagc agctcttcaa tgggatattt cttgttcagc gggaccagct
     1021 gatcgcgcaa ggcatcattc ggcgcgtgca aggacaccgc caagctcacc gggcattctt
     1081 cgcgcagacg ctccagcatc ggcacgacgc cggccgtgga caaggtcacg cgccggcgcg
     1141 aaaggccgta ggcgtaatcg tccagcatca agcgcatggc cggcaggaca ttgtcgaaat
     1201 tcagcagggg ttcgcccatg cccatgagca cgacgttggt aatgatgcgg tcgcccttgg
     1261 ggtcgcgtcc cagggcacgg ttggcgacca gcagctgcga aatgatctcg gccacggtca
     1321 gactgcggtt gaatccctga tggcctgtgg aacagaagga acaattgagg gtgcagccga
     1381 cctgcgagga cacgcacagc gtgccccggt cctcttccgg gatgaatacc gtctcgatgg
     1441 cgttaccgtc gtcgagacgc aacagccact tgcgtgtacc gtccgaggca atctggtcct
     1501 ggatgatttc cggcacgcgg atttcagcga tgtcccgcag gcgcgcgcgc aattccttgc
     1561 tcaggttggt catggcgtcg aagtcatcga cgccatactg gtgaatccac tgtatgagct
     1621 gagtggcgcg aaacggcttt tcaccaatac cggtgaaaaa ttcctgcatc gccgcgcgat
     1681 ccagattgag caaattggtt ttttcagcaa ctgacaaggc agatcctcaa cggactggca
     1741 ccggcatcag gcttgctggc gggcttcccc gccatgacat ctccggtgtt tcacaaaaat
     1801 aatgatgcaa actctggaaa aagcgggatt tttatatcca tcccaaatat atagtgtagc
     1861 ttctgatagc gggtcagaca atgaaaaaca ggctggggac ctcagcctcc gtgctcatcc
     1921 atccaagcca tctggatggc ttcgaggatt ttttcaccgg aatgttccgg catgtcgtca
     1981 aaatcctcca gccccatcac ccacttgtga aggtccgtga agcgcacgga catcgggttc
     2041 acatccgggt atttctcgct cagctcgatg gcgatctcgc gggtatcggt ccatttcagc
     2101 cccatgttcg gctccttctt aatgttgctc ggaaacaagg ttaatcgtgt atttggggat
     2161 ttcgatcacc agatcctgac tgccgacctt ggcctgacaa ctgagccgcg attcgggctc
     2221 aaggccccaa gccttgtcga gcaggtcctc ttccttttcc tcggctgtgt tcagagtgtt
     2281 aaacccctcg cgcaccacca catgacaggt ggtacaggca caggcctttt cgcaggcatg
     2341 ctcgatggga atgccggcgt tcaaggcggc atcgcagatg ctgatacccg gttccacctc
     2401 tatggtttta ccttccggac atatttgcgg gtgcggcaga aaagtcaggc gtgtcatgac
     2461 ggagattttt tccggggctc atcaaactcc tcgagcctgt gtcccgccag ggctttgcgg
     2521 atattctcgt ccatgcgacg ctgggcgaaa gtctgggtgg cctgatcgag atttttgatc
     2581 gcgcgcctga tggacgtcgc gtcatttccg gcgcgcacga catgcagctc ggccagccta
     2641 ttctcgatct cggcgcgctc gtttgtgttc agcagcgcgc cgtccacacc cagcgcgcta
     2701 cgcaccgctt ccagcaggcg atcagcctca acctgttctt cacgcagctt gcgcgtcgcg
     2761 tcgtcctcgc gtgcatgcgc aatggattca cgcagcatac gctcgatttc attgtccgta
     2821 aggccataag acggcttgac cgtcacactg ctttcaacgc ccgttgtctg ttcgcgggcc
     2881 gacacgccga gcaatccgtc ggcgtcgatc tggaatgtaa cccggatgcg cgccgctccc
     2941 gccgtcatcg gcggaatgcc acgcagctcg aagcgcgcca gcgaacggca gtc
//
LOCUS       DBNGZE010000143         1817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_576507, whole genome shotgun sequence.
ACCESSION   DBNGZE010000143 DBNGZE010000000
VERSION     DBNGZE010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_576507"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /gene="sufD"
                     /locus_tag="AC16FV_03610"
     CDS             complement(<1..1211)
                     /gene="sufD"
                     /locus_tag="AC16FV_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR2849184.1"
                     /translation="MNPNTAKETYLDAIGAAEKGLAHAPSWLRELRRGAAARFAEVGF
                     PTPRDEEWKYTDVMPIVQTGFKPVASGVNGIDLARLDGFWFENLPSHRLVFVNGHYSE
                     KLSTPGMWPAGVTVMGLAGALRHQANRIEPYLGRYVPEKIHGFAALNTALFADGAYIH
                     LGRGTRLAWPIHLLYVTTGAADPVLSQPRNLVVAEADSEATLIENHVAVGEAAYFTNA
                     LTEVVVGPHALIEHYRLQEEGASAYHIGGVHVHLDRDGRFTAHGIDLGGRLVRNDLRS
                     VLDAEGAECHLNGLYVLGGRQHVDNHTHIDHARPRGTSREFYKGVLDGRSRAVFHGRV
                     VVRPDAQHTDAQQVNNYLLLSRDAEADTKPQLEIYADDVKCAHGATVGQLDPDALFYL
                     RSRAVDEAAAR"
     gene            complement(1223..>1817)
                     /gene="sufC"
                     /locus_tag="AC16FV_03615"
     CDS             complement(1223..>1817)
                     /gene="sufC"
                     /locus_tag="AC16FV_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR2849185.1"
                     /translation="AGEISYQGRNLLELPPEERARQGMFLAFQYPVEIPGVSNIYLLK
                     SALNAIRKSQNLPELDAMDFLALVKERMKLVEMPEELLYRSVNEGFSGGEKKRNEILQ
                     MAVLEPKLAVLDETDSGLDIDALQVVARGVNVLRSPERAIILVTHYQRLLNYIEPDRV
                     HVLVDGRIAKSGDKTLAYDLEKRGYGWIAQESAPVTV"
BASE COUNT          316 a          586 c          562 g          353 t
ORIGIN      
        1 tcgcgcgccg cggcttcatc cacggcgcgc gaccgcaggt aaaacagcgc atctgggtcc
       61 aattgtccga ccgtggcgcc gtgggcgcat ttcacgtcgt cggcgtagat ttcgagctgc
      121 ggtttggtgt ccgcctcggc gtcgcgcgac agcagcaggt agttgttcac ctgctgggcg
      181 tcggtgtgct gggcgtcggg ccgcaccacg acgcggccgt gaaacacggc gcgcgagcgg
      241 ccatcgagca cgcccttgta gaattcgcgg ctggtaccgc ggggtctggc atggtcgatg
      301 tgcgtgtggt tgtccacgtg ctggcggccg ccgagcacgt acagcccgtt caggtggcat
      361 tccgcgcctt cggcgtccag cacgctgcgc agatcgttgc gcaccagccg cccgccgaga
      421 tcgatgccat gcgccgtgaa ccggccgtcg cggtcgagat gcacgtgcac cccgccgatg
      481 tgatacgcgc tggcgccctc ttcctgcagc cggtagtgct ctatcagggc atgcggaccg
      541 acgacgactt cggtcagtgc attcgtgaaa taagccgcct cgccgactgc cacatggttc
      601 tcgatcaggg ttgcttcgct gtccgcttcg gccaccacca ggttgcgcgg ctgggagagc
      661 acggggtcgg ccgcaccggt agtgacgtac aacaggtgta tcggccatgc cagacgcgta
      721 ccacggccaa gatggatgta ggcgccgtcg gcgaacaacg ccgtattcaa ggcggcaaag
      781 ccgtggatct tttccgggac gtaacggccg agatagggct cgatcctgtt cgcctgatgg
      841 cgcagcgctc cagccagacc catcaccgtg acacccgcgg gccacatacc cggggtcgac
      901 aatttttcgg aataatggcc gttcacgaaa accagacgat gtgacggcag attttcaaac
      961 cagaatccat cgagccgtgc caagtcgatg ccattaacgc cactggccac cggtttgaag
     1021 ccggtctgga caatcggcat gacgtcggtg tatttccact cctcgtcccg cggcgtcgga
     1081 aatccgacct ccgcgaaacg cgcagccgcc cctcggcgca gctcgcgcag ccaggacggg
     1141 gcgtgggcaa gccctttctc tgccgcacct atggcgtcga gataggtttc tttggctgtg
     1201 tttggattca tgggaatctc tctcagacag tcaccggcgc actttcctgc gcgatccagc
     1261 cgtagccgcg cttctcgaga tcgtaagcca gagtcttgtc gccggatttg gcgatacgcc
     1321 cgtccacgag cacgtgcacc cggtccggct cgatgtagtt gagcaggcgc tggtaatggg
     1381 tcaccagaat aatggcgcgc tccggactgc gcaggacgtt gacgccgcgc gccacgacct
     1441 gcaacgcatc gatatcgagt cccgagtcgg tctcatccag aacggcaagc ttcggctcca
     1501 gcaccgccat ctgcaggatc tcgttgcgct ttttctcgcc gccggagaag ccttcgttga
     1561 ccgagcggta cagcaattct tccggcattt ccaccagttt catccgctcc ttcaccagcg
     1621 ccagaaaatc catggcgtcg agttccggaa gattctggga cttgcggatg gcgttgagcg
     1681 ccgacttgag cagatagatg ttgctcacgc cgggaatctc caccgggtac tggaacgcga
     1741 ggaacatgcc ctgccgtgcg cgctcctcgg gcgggagttc cagcaggttc cggccctggt
     1801 aacttatctc gcccgcc
//
LOCUS       DBNGZE010000144         6431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_159156, whole genome shotgun sequence.
ACCESSION   DBNGZE010000144 DBNGZE010000000
VERSION     DBNGZE010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_159156"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1677
                     /locus_tag="AC16FV_03620"
     CDS             43..1677
                     /locus_tag="AC16FV_03620"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR2849186.1"
                     /translation="MWGLKTPVCIRPIGTDIAKAVGAPIFHVNGDDPEAVLFVTQIAL
                     DYRMTFHKDVIIDLYCYRRHGHSEADEPTVTQPLMYQRIHELPTTRALYARKLAQAGL
                     VSEDAAAGLSRDYQQRLESGANVAPRLLAREKSGYAYGADWNPYVSGPCDPATPTRVS
                     LETIRSLTERMLHVPEGLELHPQVAKIYEARRKMAAGAMPLDWGFAENLAYASLLQDG
                     YGVRLSGQDSGRGTFFHRHAVVYNQKDGSAYVPLRNLYDGQPNFLVINSLLSEEAVLA
                     FEYGYATADPKTLTIWEAQFGDFANNAQVVIDQFISSGEQKWKRLCGLALFLPHGYEG
                     QGPEHSSGRLERYLQLCAQQNMFVCVPTTPAQLFHLLRRQMRWLCRKPLVVMMPKSLL
                     RHRGSVSSLEDLTQGRFRPVLYETEALEDAAVKEVVLCSGKVYYDLAERRQALRRRDV
                     AILRIEQLYPFPEEELKQAFQRYPAARRFIWCQEEPRNQGVWYQTQHRLWRTMPQGAT
                     LEYAGRPTSAAPAVGSYQLHIAQLHALLDEALGPIK"
     gene            1696..2925
                     /gene="odhB"
                     /locus_tag="AC16FV_03625"
     CDS             1696..2925
                     /gene="odhB"
                     /locus_tag="AC16FV_03625"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR2849187.1"
                     /translation="MATDITVPVFPESVTEGTILTWRKQPGEPVRRDETLAEIETDKI
                     VFEVPAPADGVLAEIKAPVGATVKSGEVLGRVDKATSTGTTPSKPMVAEPAVQASVMP
                     AAKRLMDEHGLDARKVSGSGKGGRILKEDVLKKIDTAETAAVTPQPRPKLVPPLPATP
                     AGDRPEKRARDGAFVDQRMEKRVPMTRLRKRIAERLVEAQHTAAILTTFNEVNMQPVM
                     ELRARHRERFEKEHGVRLGFMSFFVKATVEALRHFPEVNASIDGDEVVYHGFYDVGIA
                     VSSPRGLVVPVLRDADHLTMAEIESRIRDFGERAQNAQLTLEEITGGTFTITTGGVFG
                     SLMSTPILNPPQSAILGMHKIQERPVAENGQVVIRPMMYLALSYDHRLVDGREAVQFL
                     VMIKELLEDPARLLLEV"
     gene            2928..4364
                     /gene="lpdA"
                     /locus_tag="AC16FV_03630"
     CDS             2928..4364
                     /gene="lpdA"
                     /locus_tag="AC16FV_03630"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR2849188.1"
                     /translation="MADRYDLVVIGAGPAGYTAAIRAAQLGLKVACVDDWRGPAGEPS
                     LGGTCLNAGCIPSKALLETSELFVQARDHGAGLGLKFKGLDLDLPAMLAHKDKVVADL
                     TRGIATLFKAHGVTWIPGRGKLLPKNQVEVSGDSPQQLEAENVILASGSTPVNLKSAP
                     FDGQRIVDSQAALEFQEVPRRLGIIGAGVIGLELGSVWRRLGAEVVLLEAQETFLPMA
                     DEQVAREALKQFTAQGLDIRLESRVLESKVEKNAVTTRYQGKDGEHKENVDRLIVAVG
                     RRPNTDGLVSDDARLLLDEWGFVHVDEHCRTNLPNVYAVGDLVRGPMLAHKGMEEGVM
                     VAERISGRETAVNYNVVPSVIYTQPEIAWVGKTEQALKAAGTTCRAGVFPFAANGRAK
                     AMGQAGGFIKVLADAHTDRVLGVHLIGPNCSELVTPAALAMSFGASNEDIMLTMFAHP
                     TLSEALHEASLAVAGHAIHLAQPRRKKT"
     gene            complement(4462..4538)
                     /locus_tag="AC16FV_03635"
     tRNA            complement(4462..4538)
                     /locus_tag="AC16FV_03635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4502..4504),aa:Arg,seq:ccg)
     gene            4977..5492
                     /locus_tag="AC16FV_03640"
     CDS             4977..5492
                     /locus_tag="AC16FV_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2849189.1"
                     /translation="MTDRAFWKSFAGLIAALVALTIVLFIVAQIIGGKPAKTVEATAD
                     AKAVAERIKPVGELTVGVAAEKVASSSNAVMDAIIPSANAAGAEDKGKKIYDATCMAC
                     HAAGVAGAPKLGDKANWAPRIAQGMDTLHTHAIKGFQGKAGMMPPKGGNMAAKDDDIK
                     AAVDYMVSQSK"
     gene            complement(5561..>6431)
                     /locus_tag="AC16FV_03645"
     CDS             complement(5561..>6431)
                     /locus_tag="AC16FV_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR2849190.1"
                     /translation="MIGPPGAGKTMLASRLPGILPRLNDDEALEAAAIQSLHKPFSLK
                     HWKQRPFRAPHHTASAVALVGGSSQPRPGEISLAHHGVLFLDELPDFDRHVLEVLREP
                     LESGAITISRAARQAEFPARFQLVAAMNPCPCGYYGDASGRCRCTPDKVLNYRARLSG
                     PLLDRIDMHVEVPAVSREVLLDQTSHNGESSEEVRARVEHARERQRNRSNCYNAALNN
                     QQIENTCRLDDSGRQLLEQAIDRLGLSARAYHRVLKVARTIADLAMEEAIGPAHLAEA
                     VRYRHLDRSGTLA"
BASE COUNT         1228 a         1955 c         2061 g         1187 t
ORIGIN      
        1 atacttctgc agttaagccg gtaggcgcaa aaactgacaa acatttgggg tctcaaaaca
       61 cctgtttgca tacgtccgat tggcaccgat atcgccaagg cggtgggcgc gccgatcttt
      121 cacgtcaacg gcgacgatcc cgaggcggtg ctgttcgtca cgcagatcgc gctcgactac
      181 cgcatgacct tccacaagga cgtgattatc gatctctatt gttaccgccg ccacggccac
      241 tccgaggccg acgagccgac ggtgacgcag ccgctcatgt atcagcgcat ccacgaactg
      301 ccgaccacgc gcgcgctcta cgcgcgcaag ctggcgcagg cggggctcgt cagcgaggac
      361 gcggccgctg gtctgtcacg ggactatcag cagcggctcg agtccggcgc caatgtcgcg
      421 ccgcgtctgc tggccaggga aaaatccggg tacgcctacg gcgcggactg gaacccctat
      481 gtgagcggtc cctgcgatcc tgcgacaccg acgagagtgt cgctcgaaac gatccggagt
      541 ctcaccgagc gcatgttgca cgttccggag ggcctagaac tgcacccgca ggtcgccaag
      601 atctacgagg cgcggcgcaa aatggcggcc ggtgccatgc cgctcgactg gggcttcgcc
      661 gaaaacctcg cctatgccag cctgttgcag gatggttacg gggtgcggct gtcaggccag
      721 gattcgggac gcggcacgtt ctttcatcgc catgcggtgg tgtacaacca gaaggacggc
      781 tcggcctatg tgcccttgcg caatctttac gacgggcagc cgaattttct ggtcatcaat
      841 tcgctgctgt ccgaagaggc cgtgctggcc ttcgagtacg gttacgccac ggccgatccc
      901 aagaccctca ccatctggga ggcgcagttc ggcgatttcg ccaacaacgc gcaggtggtc
      961 atcgaccagt tcatcagctc gggcgaacag aaatggaagc gcctgtgcgg gcttgcgctg
     1021 tttctgccgc acggctacga aggccagggg ccggagcatt cctcggggcg gctcgaacgc
     1081 tatttacagc tgtgcgcgca gcagaacatg ttcgtgtgcg tgccgaccac gccggcgcag
     1141 ctgttccatc tgttacggcg ccagatgcgc tggttgtgcc gcaagccgct ggtggtcatg
     1201 atgccgaaga gcctgctgcg ccaccgcggt tcggtgagca gcctcgagga tctgacccag
     1261 ggacgtttcc gcccggtgct gtacgaaacc gaggcgctgg aggacgccgc cgtgaaggag
     1321 gtcgtcctgt gcagcggcaa ggtctactat gatctggccg agcgccgcca ggctttgcgg
     1381 cgccgcgatg tcgcgatcct gcgcatcgag cagctttatc cgtttccgga ggaggaattg
     1441 aagcaggcgt ttcagcgcta tcccgccgcg cgccgtttca tctggtgcca ggaggagccg
     1501 cgcaaccagg gcgtctggta ccagacccag catcgcctgt ggcgcacgat gccgcagggc
     1561 gcgacactcg aatacgccgg ccgcccgacc tcggccgcac cggccgtggg cagctatcag
     1621 cttcacatcg cccagctgca tgcgttactg gatgaagcgc tgggaccgat caaataatgg
     1681 cgctcggagc acagcatggc cactgacatc acggttccgg tttttccgga atcggtcacc
     1741 gaaggcacga tcctcacctg gcgcaagcag ccgggcgagc cggtgcgacg cgacgagaca
     1801 ctggccgaga tcgaaaccga caagattgtt ttcgaggtcc cggcgcccgc tgacggtgtg
     1861 ctggccgaga tcaaggcgcc ggttggcgcg accgtgaaat cgggcgaggt gttaggccgg
     1921 gtcgacaagg ccacatccac tgggacgacc ccttccaaac ccatggtcgc ggagcccgca
     1981 gtccaggcgt ctgtcatgcc cgccgcgaaa cgtttgatgg atgagcatgg tttggatgcg
     2041 cgcaaggtat cgggtagcgg caagggcggg cgcattctca aggaagacgt gctgaaaaaa
     2101 atcgataccg cagagaccgc ggcggtgacg ccgcagccca gacccaagct ggttccaccc
     2161 ctgcccgcca cgccggccgg agaccgtcct gagaagcgcg ccagggatgg cgcgtttgtg
     2221 gaccaaagga tggagaaacg cgtgcccatg acgcgcctgc gcaagcgtat cgccgaacgc
     2281 ctggtcgagg cgcagcacac ggcggcgatc ctgacgacct tcaacgaggt caacatgcag
     2341 ccggtgatgg aattgcgcgc gcgccatcgc gagcgcttcg agaaggaaca cggcgtgcgc
     2401 ctcggcttca tgtcattctt cgtgaaggcc acggtcgagg cgctgcggca tttccccgag
     2461 gtcaatgctt ccatcgatgg cgatgaagtg gtgtatcacg gcttctacga cgtcggcatc
     2521 gcggtcagtt caccgcgcgg gctggtggtg ccggtgttgc gcgacgccga ccacctgacc
     2581 atggccgaga tagagtcgcg catccgtgat ttcggcgagc gtgcgcagaa tgcgcagctc
     2641 acgctcgagg agattaccgg cgggaccttc accatcacca ccggcggggt gttcggctcg
     2701 ctcatgtcca ccccgatcct gaatccgccg cagagcgcga tcctcggcat gcacaagatc
     2761 caagaacgcc cggtggcgga aaacggccag gtcgtgatcc ggccgatgat gtatctggcg
     2821 ttgtcctacg accaccgctt ggtggacggg cgcgaggcgg tgcaatttct ggtgatgatc
     2881 aaggagctgc tggaagaccc ggcgcgcctg ctgctcgaag tctgaacatg gctgatcgct
     2941 acgatcttgt cgtcatcggc gccggtccgg cgggctacac cgcggccatc cgtgccgccc
     3001 agctcggcct caaggtggcg tgcgtggacg actggcgcgg accggccggt gagccttcgc
     3061 tcggtggtac ctgcctcaac gccggttgca tcccgtccaa ggcgttgctc gaaacctctg
     3121 agctttttgt gcaggcgcgc gaccatggcg ccggcttggg cctcaagttc aaggggctcg
     3181 acctcgattt gccggccatg ctggcgcaca aggacaaggt ggtggcggat cttacgcgtg
     3241 gcatcgccac gttgttcaag gcccatggcg tgacctggat tccgggtcgc ggcaaactcc
     3301 taccgaaaaa tcaggtggaa gtgtccggag attccccgca gcaactggaa gcggaaaacg
     3361 tgatactcgc gagcggctcg acaccggtga atctcaagag cgcacctttc gatgggcagc
     3421 gcatcgtcga ttcgcaggcg gcgcttgaat tccaggaagt gccacggcgc ctaggcatca
     3481 tcggcgccgg cgtgatcgga ctcgagctcg gcagcgtgtg gcgccggctc ggtgcggagg
     3541 tggtgctgct cgaggcacag gagactttcc tgcccatggc cgacgagcag gtggcgcgcg
     3601 aggcgctcaa gcagttcacc gcgcaggggt tggatatccg tctcgagtcg cgggtactgg
     3661 aaagcaaggt cgagaagaac gcggttacga cgcgctatca aggcaaggac ggtgagcaca
     3721 aggaaaacgt ggaccggctc atcgtcgcgg tcggacgccg gcccaacacc gatggtcttg
     3781 tcagcgacga cgccaggctg ctgctggacg aatggggttt cgtgcatgtc gatgagcact
     3841 gtcgcacgaa ccttccgaac gtctatgccg tgggcgatct ggtgcgcggg ccgatgctgg
     3901 cgcacaaggg catggaagaa ggcgtgatgg tggccgagcg catcagtggt cgtgagaccg
     3961 cggtgaatta caacgtcgtg ccctcggtga tttacaccca gcccgagatc gcctgggtcg
     4021 gcaagaccga gcaggcgctg aaggccgcgg gcacgacctg ccgcgccggc gtgtttccct
     4081 tcgccgcgaa cgggcgcgcc aaggccatgg gccaggccgg tggcttcatc aaggttctcg
     4141 ccgacgcgca taccgatcgc gtgctcggcg tgcacttgat cggcccgaac tgttctgaac
     4201 ttgtcacgcc ggcggcactg gccatgtcct tcggtgcttc gaacgaggac atcatgctca
     4261 ccatgttcgc ccaccccacg ctttcggagg ccctgcacga ggcgtcgctc gccgtcgcag
     4321 gtcatgcgat tcatctggcg cagccgcggc ggaagaagac gtgaatggcg ccctattgga
     4381 ttttatgaat cggtcagagg ttcaaatcct gcaagtactt catcaaagcc aaaggcccca
     4441 catgggggcc tttggctttg atggcgcgcc ctagaggatt cgaacctctg accttcagcg
     4501 tcggaggctg acgctctatc cagctgagct aagggcgcat gatcaacaat ggtaacactt
     4561 ggaatctcgg cctctgaagt acttcgcgtc agcggctgac gctcgttctc gcaatacgtg
     4621 catgccatcc ctggcgcggc ttctcaacca gtgttagacc ccagcctcgc catccatggc
     4681 gaggcgctcg cggctcctaa gcccgccgct cgcccacagg accttcgcga accccccggt
     4741 tcacccagct gagctaaggg cgcaaaaata aactaatagg gttatgactt gatctgggtc
     4801 atttccgtcg tggaacgggg acgcaagaat acctgtcgta gtgggcaacg tctatctgtc
     4861 gcaggcggag gggctttttc agaacgaata ttaggctata ctaatgcggc aaattcagga
     4921 cccccaccat tcggtagggg aaaaacttca acattgaaat tggttaaagg taaatcatga
     4981 ctgatcgtgc attctggaaa tcttttgcgg gcctgatcgc tgcgctggtg gcgttgacga
     5041 tcgttctctt catcgtggcg cagatcatcg gcggcaagcc ggcgaagact gtcgaagcca
     5101 cggcggatgc caaggctgtc gccgaacgta tcaagcccgt gggtgaactg accgttggtg
     5161 tcgcggcgga gaaagtcgcc agctcctcca atgcggtgat ggatgccatc attccttccg
     5221 ccaatgccgc gggtgccgaa gacaagggta agaaaatcta cgacgcgacc tgcatggcct
     5281 gccatgcggc cggtgtagcc ggtgcgccga agctgggtga caaggcgaat tgggctccgc
     5341 gtatcgctca gggtatggac acgctgcata cccatgccat caagggcttc cagggcaagg
     5401 ccggcatgat gccacccaag ggtggcaaca tggctgccaa ggatgacgac atcaaggctg
     5461 ctgtcgatta catggtgtcg cagagcaagt aagtcttttc gttttcagta ataaaaaagc
     5521 ggccttgcgg ccgctttttt atttgtccgg aaaatttgtc tcaggcaagc gttccactcc
     5581 ggtcgagatg gcgatatcgc actgcttccg ccagatgcgc cggaccaatg gcctcttcca
     5641 tggcgaggtc ggcgatggtg cgcgccacct tcagcacgcg atgataggcg cgcgccgaaa
     5701 gtccaagccg gtcgatggcc tgttccagca attgccgccc tgaatcatcc agccgacagg
     5761 tattttctat ctgctgatta ttgagcgcgg cgttgtaaca gttgctgcgg ttgcgctgcc
     5821 gttcacgcgc atgttccacc cgggctctca cctcctctga ggattcgccg ttgtgtgatg
     5881 tctgatccag aagcacttcc cgtgacaccg ccggcacttc cacgtgcatg tcgatccggt
     5941 ccagcaaggg cccggaaagg cgcgcgcggt aattgagaac cttgtccggc gtgcagcgac
     6001 agcgtccgga agcgtcgccg tagtaaccgc agggacaggg gttcatcgcc gcgaccagct
     6061 gaaaccgggc aggaaactcc gcctggcgcg cggcacgcga aatggtgatc gcgcccgatt
     6121 ccagcggctc gcgcagtact tcaagcacat gccggtcgaa gtccggtaac tcgtccagaa
     6181 acagcacgcc gtgatgcgcc agcgaaatct cgcctggacg cggctggctg ctgccgccga
     6241 ccagggccac ggcggaagcg gtgtgatgcg gcgcgcggaa cggccgctgt ttccagtgct
     6301 tgagggagaa cggcttgtgc agcgactgga tggcggcggc ctccagcgct tcgtcatcgt
     6361 tgaggcgcgg cagtatgccc ggcagacgcg aggccagcat ggtcttgccc gcacccggcg
     6421 ggccgatcat g
//
LOCUS       DBNGZE010000145        10850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1058147, whole genome shotgun sequence.
ACCESSION   DBNGZE010000145 DBNGZE010000000
VERSION     DBNGZE010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1058147"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..2273)
                     /locus_tag="AC16FV_03650"
     CDS             complement(315..2273)
                     /locus_tag="AC16FV_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR2849191.1"
                     /translation="MLIALLIVGAMAVSPFFQGFEAALLSTVVAALLLALLMTLWQSY
                     DTGLALPRHPLPVALLLLWVWFAVSLAWHPVVYIGVYNFWWLGTFPIAFAWYWLWREH
                     PLCRPVTRISAALLGAGLAGYAVIQFFAFGLPPRSVFININAHAALLNLILLPAVGFF
                     LVAATKRKPDRRVQFALAVLIALLAFAIALTRGRGSMLSLAAGLALISFIAWRKLPTR
                     VWITPLALCLAAFAVANLAWQGGVTERYAELADPMGAGSMQARFMIWEGAWQLLREAP
                     WFGIGLGMFALVYPVVRAPQDSSAGFLVHNDYLQLWLEAGLPALLLLLAAMLAVTVLF
                     VRAQRSPKLSKAAKLEAAGLFAGVLAVSMHTAVDFNFFVLPILMLMGFMLARLLELGG
                     KSAGVRIAVWRPSGMLSAGGWRTLLVTVFFFPAVYFISAPASIYYTDKGVALAAQGNL
                     IEAEDALMFASGLYPFADNIYISQADLYRYMLTLMPADTDDDRKRIYDAAHALLDRAE
                     RLNPYRPLNFLVRGRLYAENRALAGDSAVPMAVEAWRRALALNPRFYPARIAWANLLI
                     TEGDTAAARALVEDGLREYYLVHGGIVPYFALAGRLRADAGDKAGAAEMGERIKEAMA
                     ASGWRWVPLPENAPVLERPEKLVEKSGS"
     gene            complement(2445..2870)
                     /locus_tag="AC16FV_03655"
     CDS             complement(2445..2870)
                     /locus_tag="AC16FV_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR2849192.1"
                     /translation="MYKQQSGFTLIELVIVIVILGLLAATALPRFANLTQDARIATLG
                     GMKGALPSAATLAHATQLAKGLASNVSVSMDGQTVTMANGYPTADAPGIVAALTDYTG
                     FTPTGGVFAVNGTTSNCTATYVASAGGVFPQVTLATSGC"
     gene            complement(2974..3537)
                     /locus_tag="AC16FV_03660"
     CDS             complement(2974..3537)
                     /locus_tag="AC16FV_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR2849193.1"
                     /translation="MSRGSQNLGGCGYTRRCHGGFSLLELVIVIVIISVLLVLAISRL
                     LALMVDAERVTMETVAGTLRSAIGMKVANNIVKLKVSELPAFEGSNPMVLLAETPRNY
                     LGELDGADPAKLEDGNWYYDKHDKALVYLVHHKGFFSGGQANPPRARFAVRLVYSDSN
                     RNGVFDAGVDEIQGLRLSAMESYSWSR"
     gene            complement(3725..4864)
                     /locus_tag="AC16FV_03665"
     CDS             complement(3725..4864)
                     /locus_tag="AC16FV_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR2849194.1"
                     /translation="MPLFQYKGRNQRGEAVRGHLEAVSPDAVALQLMNSGVIPIDIGI
                     AQVREDVFAALKARLAAGTRITLTDLSLFSRQMYTLLKAGVPIMQALRGLRDSTQNPA
                     LAKVIISISESLDMGLDLTGALRRHPRVFSSLYVNMVQVGETTGSLQEVFLQLAVYLE
                     REKDTRDRIKSATRYPIFVVAAIFIAIFIINMFVIPTFAKVYAGFKIELPMATKILIA
                     SSNFTVAYWPVLLTVIIVTIFALRYYVHTPEGRYRWHKMKLGIPVVGKVIYKATLGRF
                     SRALAVMMKSGVPLVQGMTVVSRAVDNDYISDRIVQMRDGVERGETIARTAAATGMFP
                     PLVIQMIAVGEDTGSVDELMFNVADYYEREVDYDIAISAPPSSRS"
     gene            complement(4989..6710)
                     /locus_tag="AC16FV_03670"
     CDS             complement(4989..6710)
                     /locus_tag="AC16FV_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR2849195.1"
                     /translation="MATITEMKPKKIRIGDLLIANKVISQEQLNTALADQKKSGRKLG
                     RVLVENGFISEDQLLTFLSQQLNIPYIDLRRYHYKPETVRLIPETHARRFRAIALEDS
                     RESLLVGMADPTDIFAYDELSRVLRRSLRFAVVKESELLKTIDRVYRRTEEISDLAQE
                     LQQELTAQAGVDLAALAEAEGITDAPVVKLLQSMFEDAVQVNASDIHIEPDEKELRIR
                     FRLDGVLRVQTVADRRIASALVSRVKLMANLDISEKRLPQDGRTNIRIRDKNIDVRIS
                     TMPVQYGESVVLRLLNPFSGVLSLEQVGMPKNLLERFRRLIHNPNGRVLVTGPTGSGK
                     TTTLYAALKELNKPEFKILTVEDPVEYRLTGINQVQVNTKIELTFARVLRSMLRQDPD
                     IILVGEMRDQETAEIGLRAAMTGHLVLSTLHTNDAISTALRLTDMGVEPFMVAASLRG
                     IISQRLVRRICESCAEPHELPAAQMAIVRAEAGDKADTLKFKRGRGCSHCNGTGYQGR
                     IGVFEYLEMDNALVDALHAKDTGKFEAAAKQQPGFQTLRRAAIILAAQGLTTMDQVVR
                     ATYSIEE"
     gene            7060..7524
                     /gene="trmL"
                     /locus_tag="AC16FV_03675"
     CDS             7060..7524
                     /gene="trmL"
                     /locus_tag="AC16FV_03675"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR2849196.1"
                     /translation="MFHIVLYQPEIPPNTGNIMRLCANTGARLHLIEPLGFRLEDKEL
                     RRAGLDYREWADVKVHANFEVFLESVKPLRVFAFSTRAQRLYTETEYKANDALLFGPE
                     TRGLPQDLLNSLPGEQKLRLPLLPGNRSLNLSNAVAVVVYEAWRQQGFSGGK"
     gene            complement(7590..8603)
                     /locus_tag="AC16FV_03680"
     CDS             complement(7590..8603)
                     /locus_tag="AC16FV_03680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR2849197.1"
                     /translation="MSSAKKHTIAVLGAGSWGTALAMLLARNGHTALLWGNDAARQAR
                     LEHERQNSVYLPGIPFPANLHVRDALAEVAKSSSRFLVAVPSHAFHATLAALKPLLSA
                     GTVLAWATKGLEPGSGKLLSQVAQEVLDDERPRAVISGPTFAREVALGLPTALAVASE
                     SESVAEEVASWLRNDRVRVYTSSDVAGVQLGGAIKNVMAIAAGISDGLGFGANARAAL
                     ITRGLAELTRLGIAMGGQKETFMGLAGAGDLILTCTDDASRNRRVGLALGQGKKLPEI
                     LSGLGQEAEGVATARELYQLAKRSNVDMPITEQVYRVLYEDLPPQAAVEALLRREPRQ
                     EHQ"
     gene            complement(8727..9197)
                     /gene="secB"
                     /locus_tag="AC16FV_03685"
     CDS             complement(8727..9197)
                     /gene="secB"
                     /locus_tag="AC16FV_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR2849198.1"
                     /translation="MAEADQQQNTVFSIEKIYLKDVSYEAPGVPLAFSQSQNAGAEIG
                     VQLGLEHSILAAEQGLYEVVLTITATAKRENKNIFLVEVKQAGIFRIGGVNNEMLRRA
                     LEISCAYALLPFAREAVNDFVEKGGFPQLLITPINFEALYEQKQSAQASQPAIN"
     gene            complement(9239..9493)
                     /gene="grxC"
                     /locus_tag="AC16FV_03690"
     CDS             complement(9239..9493)
                     /gene="grxC"
                     /locus_tag="AC16FV_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HIR2849199.1"
                     /translation="MAKVLMYSSRLCPYCRMAERLLEQKGIQAEKVMVDEDPARREEM
                     TRRAGRTSVPQIFIGETHVGGYTELAGLERAGQLDTLLKS"
     gene            complement(9496..9909)
                     /locus_tag="AC16FV_03695"
     CDS             complement(9496..9909)
                     /locus_tag="AC16FV_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2849200.1"
                     /translation="MTQFIIHNWYLFVALAVILFLLATGPISQRMYGIKNANAAQTVQ
                     LLNREHGVVVDVCEPKEFSAGHVPNAINLPLSSLKDRLRDLDKYKNKPVIVSCRSGNR
                     SLKGAVLLRKHGFPTVYNLSGGLQAWERDNLPVEK"
     gene            complement(10032..10367)
                     /locus_tag="AC16FV_03700"
     CDS             complement(10032..10367)
                     /locus_tag="AC16FV_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2849201.1"
                     /translation="MSAAAEKVEDVLITNEDDIHLASRSLKAMAHPLRLKILCILGGH
                     AEVSVQDIVEQVGTSQSNISQHLSILRDKGILAARKDANKVYYRISDPKILQLIGSLR
                     EAFCSAVRR"
     gene            10516..>10850
                     /locus_tag="AC16FV_03705"
     CDS             10516..>10850
                     /locus_tag="AC16FV_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330205.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR2849202.1"
                     /translation="MSFTPKPLVLILLDGWGYRQDTQYNAIAAARKPVWDMLWKQYTH
                     TLIHASEAAVGLPSSQMGNSEVGHLNLGAGRVVYQEYTRINRAISTGTFFTNKTLTDT
                     IDLAVKTGKA"
BASE COUNT         2351 a         3307 c         3044 g         2148 t
ORIGIN      
        1 agcccgcgtg ccgatatacc atggaacctc gacctgccat tttcatcgga aaacaagcaa
       61 agtttagacc accgggagga aacggcgcct gaactcacat tcgccgctag acctcatcca
      121 cgctctaaga gaccgtaaca aaatgaaaaa tgacgccatg atgccgaaaa ttagagttcc
      181 ttctcccgcc gggagagggg ttcgtgccga tgttcatttt gttacggacc ctaagtggct
      241 cattgccgga ggtttccggt cagcactcag tcgcgccacc cagctaatgg gtgcaaagta
      301 aaacccggac aggctcaaga tcccgacttt tccaccaatt tttcgggccg ctcaagcacc
      361 ggggcatttt ccggcagcgg cacccagcgc cagccggagg cggccatggc ctccttgatg
      421 cgttccccca tttccgccgc ccccgccttg tcaccggcgt ctgcgcgtag ccggcccgcg
      481 agcgcgaaat agggcacgat gcccccatgc accaggtagt actcgcgcag gccatcctcg
      541 accaacgccc tcgccgccgc ggtatcgccc tcggtgatga gcagattggc ccaagcgatg
      601 cgtgcgggat aaaagcgcgg gttgagcgcg agtgcgcgcc gccaagcttc aaccgccata
      661 ggtacggcgc tgtcgccggc gagcgcgcga ttctcggcgt agagccgacc acgcaccagg
      721 aaattcagcg ggcggtaggg gttgagtcgc tcggcccgat cgagcagcgc atgcgccgcg
      781 tcgtaaatcc gcttgcggtc atcgtcggtg tcagccggca tgagcgtgag catgtagcgg
      841 tagagatcgg cctgactgat atagatgtta tcggcaaagg gataaagccc cgaggcgaac
      901 atgagcgcat cctcggcctc gatcaagttt ccttgcgcgg cgagcgcaac acccttgtcg
      961 gtgtagtaaa ttgacgccgg cgccgaaatg aaatacacgg ccggaaagaa aaaaacggtg
     1021 acaagcaagg ttcgccaacc gccagcgctt aacatgcccg atgggcgcca gaccgcgatg
     1081 cgcacgccag cgctcttgcc cccaagctcg agcaggcgcg caagcatgaa acccatcagc
     1141 atcaggatcg gcaacacgaa gaaattgaaa tccaccgcgg tgtgcattga caccgccagt
     1201 acaccggcaa acaaccctgc agcttccagc ttggctgctt tgctgagttt gggcgagcgc
     1261 tgggcgcgca cgaacaacac ggtcaccgcc agcatcgcgg cgaggagaag cagcagcgcc
     1321 ggcagcccgg cctcgagcca aagttgcaga tagtcgttgt gtacgagaaa tcctgcgctc
     1381 gaatcctgcg gcgcccgcac caccggatag acgagcgcga acatgcccag accgatgccg
     1441 aaccagggcg cctcgcgcaa cagttgccag gcgccttccc agatcatgaa ccgcgcctgc
     1501 atcgagccgg cccccatggg gtcagccagc tccgcgtagc gctcggtcac gcccccctgc
     1561 caggcgagat tcgccaccgc gaaggcggcg agacacaggg cgagcggcgt tatccagaca
     1621 cgcgtgggta acttgcgcca agcaataaat gaaatgaggg cgaggccggc ggccagtgac
     1681 aacatcgatc cccggccccg agtcagggca atggcaaaag caagcagcgc gatcaggacg
     1741 gcgagcgcga attgcacgcg ccggtccggc ttgcgttttg tcgcggcaac cagaaaaaac
     1801 cccacggctg gcaacaggat caggttgagc agcgcggcgt gcgcgttgat gttgatgaac
     1861 accgagcgcg gcggcaaccc gaacgcgaaa aactgaatca ctgcgtaacc ggccagtcca
     1921 gcgccgagca gagccgcgga aatccgggtg acgggccggc acaatggatg ctcgcgccac
     1981 aaccagtacc acgcgaaggc aatggggaac gtaccgagcc accagaaatt gtaaacaccg
     2041 atatagacga ccgggtgcca tgcgaggctc acggcaaacc acacccacag cagtaacagc
     2101 gccacgggga gcggatggcg cggcaaggca aggccggtgt catatgactg ccacagcgtc
     2161 atcagcaggg ctaacagcaa cgccgccacg acggtggaaa gcaatgccgc ctcgaagcct
     2221 tgaaagaatg gcgatacggc catcgccccg acgataagca gggcgattaa catgctggca
     2281 gaacggtgct gaaaatcaga catgggcgtc atcgcacacg ggaaccatca aacgcgcacg
     2341 aagggaaggc aacgacttgg catatccata tgctcatgta gcacacaaaa aaaggatgga
     2401 cgcgctatgc gtccatccct ttggtgacag gcaacttgta tttttcagca gccgctggtg
     2461 gcaagcgtga cctgcgggaa tactccaccg gccgacgcca cataggtcgc tgtgcaattc
     2521 gatgtggtcc cattcacggc gaagactccg ccggtcggcg tgaagcccgt gtaatcggta
     2581 agcgctgcta cgatgcccgg ggcatcggcc gttgggtagc cattcgccat cgtgaccgtc
     2641 tgaccatcca tcgatacgct gacatttgac gctaggccct tagcaagctg ggtggcatgg
     2701 gcgagggtgg ccgcgctcgg cagggcgccc ttcatgccgc ccagcgtcgc gatgcgcgca
     2761 tcctgcgtca ggttcgcgaa ccgtggcagc gccgtggccg ccagcaggcc aagaatgacg
     2821 atgacgatga cgagttcaat aagagtaaaa ccgctttgct gtttgtacat gatcaatctc
     2881 ctgcgtttca aaacaagttg ctctatcacg ttacttcagc ggccagatcc gtcaccaacc
     2941 gccgtccttt accacccgcc ggccccgcat ccttcatcgg gaccaggagt aggattccat
     3001 cgcgctcaga cgcagaccct gtatctcatc cacgccggca tcgaaaacac cgttcctgtt
     3061 actgtcactg tagaccaatc tcaccgcgaa gcgcgcccgc ggggggttgg cctgcccgcc
     3121 actgaaaaaa cccttgtggt ggaccagata aacaagcgcc ttgtcgtgct tgtcgtaata
     3181 ccagttgccg tcctcgagtt tcgccgggtc agccccgtcc agctcgccca gatagttgcg
     3241 cggcgtttcc gccagcagca ccatcgggtt actaccctcg aaggctggca attccgaaac
     3301 cttcaacttc acgatattgt ttgcaacctt catgccaatg gcgcttctga gcgtccctgc
     3361 cactgtctcc atggtcacgc gctcggcatc caccatcaga gccaataagc gcgaaatcgc
     3421 tagcaccagc aacaccgaaa tgatcacgat cacaatgacc agttcgagca gggaaaagcc
     3481 gccatgacaa cgccttgtgt acccgcagcc acccagattc tggcttcccc ggctcatttt
     3541 ttgtcccatc ccgcaaagct aggcagccca agaaaacagt aggttacgaa tccaagcttg
     3601 tccagattct cagtactggc cataaggcgt caggactagc ctttgcgcgc gacctggacc
     3661 aggtcccaca tcggcaggaa cacgcccagc gccaggatga acaccatgat ggccaggaac
     3721 acgatcagga tcggctggat ggcggtgctg agattgcgat gtcgtagtcc acttcgcgct
     3781 cgtaatagtc cgccacgttg aacatgagtt cgtccaccga gccggtgtcc tcgcccaccg
     3841 cgatcatctg gatcacgagt ggaggaaaca tgccggtggc ggcggcggtg cgggcgatgg
     3901 tctcgccgcg ctcgacccca tcgcgcatct gtacgatgcg atcgctgatg tagtcgttgt
     3961 caacggcgcg gctgacgacg gtcatgcctt gcaccagcgg cacgcccgac ttcatcatca
     4021 ccgccaacgc gcgggaaaac cgtcccagcg tcgccttgta aatcaccttg ccgacgaccg
     4081 ggatccccag cttcatcttg tgccatcgat accggccttc cggcgtgtgg acgtaataac
     4141 gcagcgcgaa gatggtaacg atgattacgg tcaggagtac cggccaatag gcaacagtga
     4201 aattggagga ggcgatgaga atcttggtcg ccatgggcaa ttcaatcttg aatccggcat
     4261 acaccttggc gaaggtcgga atcacgaaca tgttgatgat aaatatggcg atgaaaatcg
     4321 ccgctacgac aaaaatgggg taacgtgtcg cagacttgat acggtcacgg gtgtcctttt
     4381 cccgttccag ataaactgca agttgtaaga aaacctcctg caggctgccc gtggtctcgc
     4441 ccacctgaac catgttgaca tacagggatg aaaacacccg cggatgccgg cgcaatgcgc
     4501 cggtaagatc gaggcccatg tcgaggctct cgctgatgct gatgatgacc ttggccaaag
     4561 ccgggttctg ggtcgaatcg cgcaagccgc gcagggcctg catgatcggc acgccggcct
     4621 tgagcagggt gtacatctga cgactgaaga gcgacaggtc cgtcagcgtg atgcgggtgc
     4681 cggcggccag gcgcgccttg agtgcggcga aaacatcctc ccgcacctgg gcgatgccga
     4741 tatcgatcgg aatgacgccg ctgttcatca gctgcagcgc cacggcatcc ggcgaaacgg
     4801 cttcgagatg accccgcacc gcctcaccac gctgattgcg ccccttgtac tggaataacg
     4861 gcataaatga agccctactc gctccgctcg ttcgtcttga gataagacat cgccccgctt
     4921 cgctcgtcct gatatatgac tagtggccct actcgtttcg ctcgttcgtc ttgatataaa
     4981 acataaaact attcctctat cgaataagtg gcgcgcacca cctgatccat ggtggtcaga
     5041 ccctgcgccg ccaaaatgat ggcggcgcgt cgcaaggtct ggaatccggg ctgttgtttg
     5101 gccgcagcct cgaacttgcc ggtgtccttc gcgtgcaagg catccaccaa cgcgttgtcc
     5161 atctcgagat attcgaaaac gccgatacgg ccctggtagc cggtgccgtt gcaatgactg
     5221 cacccgcgcc cgcgcttgaa tttgagcgta tccgccttgt ccccggcctc ggcccgcacg
     5281 atggccatct gcgccgcagg cagttcatgg ggttcggcgc aggactcaca aatgcgccgc
     5341 accaggcgtt gcgaaatgat gccgcgcagc gaggctgcga ccataaatgg ctccacaccc
     5401 atgtcggtca ggcgcagcgc agtcgaaatc gcatcgttgg tatgcagggt ggacagcaca
     5461 aggtggcccg tcatggcggc acgcaggccg atttccgccg tctcctggtc gcgcatttca
     5521 ccgaccagga tgatgtccgg gtcctggcgc agcatggatc gcagcacgcg ggcgaacgtc
     5581 agctctatct tggtgttgac ctgaacctga ttgataccgg tcagacggta ttccaccggg
     5641 tcctcgacgg tcagtatctt gaattcgggt ttgttcagct ccttgagcgc cgcatacaac
     5701 gttgtggttt tgccgctgcc cgtcgggccg gtgaccagca ccctgccatt ggggttgtga
     5761 atcagccggc ggaaacgctc gagaaggttc ttcggcatgc ccacctgttc cagactgagc
     5821 acaccgctga acgggttcag cagacgcagc accacggact caccgtattg cacaggcatg
     5881 gtcgagatac gtacgtcgat gttcttgtcg cggatacgta tgttggtgcg gccgtcctgc
     5941 ggcaaacgct tctccgagat atcgagattc gccatcagtt tcacgcgcga caccaatgcg
     6001 ctggcaatgc gccggtcggc aacggtctgc acccgcaaca cgccgtcgag gcggaaacgg
     6061 atgcgcaact ccttctcgtc cggctcgatg tgaatgtcgg aggcgttcac ctgcaccgcg
     6121 tcctcgaaca tgctttgcag caatttgacg accggcgcgt cagtgatgcc ctcggcctcg
     6181 gccagggccg ccagatccac gccagcttgg gctgtcaatt cctgttgcag ctcctgcgcc
     6241 aggtcactga tctcttccgt acgccggtag acacgatcaa tggtcttgag caattcggat
     6301 tccttgacca cggcgaagcg cagcgaacgg cgcagcacgc gtgacaattc gtcgtaggca
     6361 aagatatccg tgggatcggc catgcccacc agcaggcttt cgcggctgtc ttccagcgct
     6421 atagcgcgga aacggcgggc atgtgtctcg ggaatgaggc gaaccgtttc gggcttatag
     6481 tgatagcgcc gcagatcaat ataaggaatg ttgagctgct gcgagaggaa ggtgagcagc
     6541 tgatcctccg agataaaacc gttctccacc agcacgcgtc cgagtttgcg gccgcttttt
     6601 ttctggtccg ccagcgcggt gttgagctgt tcctgcgaaa tgactttatt cgcaatcagc
     6661 aagtcgccga tgcgaatctt cttgggtttc atttcggtta tcgttgccat cgtgtttctc
     6721 cgctgggcca cctcttaaat cccgcgaatg aggcgcagcc taatacactg aaaatattga
     6781 cctaacccca ctatagccga aaaaatcggt gttcccggtt agcgacagga cagaatccga
     6841 ccctccagat tcattgcagg ggcctaaata tccctgaatc actagcaaat accgagccag
     6901 ctttaagcgc agggtatggc ggacaggcag gagcaatacc cgcacaggtg acggccagtc
     6961 atgatcggtt ttccgacggg acgggagacc acaggtttct tatctatata caatccgcca
     7021 taaagcaaca gttcacaccc gcgccaaggc gacccagtca tgtttcatat cgtgctctat
     7081 caacccgaga ttccacccaa caccggtaac atcatgcgtc tgtgcgccaa taccggcgcg
     7141 cgcctgcatc tgatcgaacc gctcggcttc cgtctggaag acaaggaatt gcggcgcgcg
     7201 gggctggatt accgcgagtg ggcggatgta aaagttcacg cgaacttcga ggtctttctt
     7261 gagagcgtga aaccgttgcg cgtgttcgcg ttctccacgc gcgcgcaacg gctctatacg
     7321 gagactgaat acaaggcaaa cgatgccctg ctctttggtc ccgaaacgcg cggattgccc
     7381 caagacttgc tgaattcact cccgggagaa cagaagttgc gactgccgct tctgcccggc
     7441 aaccgcagtt tgaatctctc caacgcagtc gcggtggtgg tttatgaagc ctggcgacaa
     7501 caggggttca gcggcgggaa atgattatta tcttcagacg agcgcagcag ggcgacgctt
     7561 agtcacatcc agacgaacga aaaacagggt tactggtgct cctgtcttgg ctcgcgccgg
     7621 aggagcgcct ccaccgcggc ctggggcggc aggtcctcat acaacacgcg atacacctgc
     7681 tcggtgatgg gcatatcaac gttcgatctt tttgcgagtt gatacagctc acgcgcggtg
     7741 gccaccccct cggcttcctg gccgagtcca gaaagaatct cgggtagttt tttcccctgc
     7801 ccaagcgcca gaccaacgcg acggttacgc gaagcatcgt cggtgcaagt caatatcaga
     7861 tcgcccgcgc cggcgagtcc catgaaggtt tccttttgcc cgcccatggc gatgccaaga
     7921 cgtgtaagct cggccagccc gcgagtgatg agcgccgcgc gtgcgttggc cccgaaaccg
     7981 aggccatcgc tgatgccggc ggcgatggcc atgacattct tgatggcgcc accgagctgc
     8041 acaccggcaa cgtcgctact ggtgtagaca cgcacgcggt cgttgcgcaa ccaggaggcg
     8101 acttcttccg ccactgactc actttccgag gcaaccgcca gcgccgtcgg caatccaagc
     8161 gccacttcgc gtgcaaacgt cggtcctgaa atcacggcgc ggggccgctc atcatcgagc
     8221 acctcttgcg ccacttggct cagcaatttc ccactgccgg gttccagccc tttcgtggcc
     8281 caggcgagca cggtgcccgc ggacaacagc ggcttcaagg ccgcgagtgt ggcgtggaag
     8341 gcgtggcttg gaaccgcaac cagaaaacgc gaggaggatt tggcgacctc agcgagcgca
     8401 tcgcgcacgt gcagattcgc cgggaaggga atgccgggga ggtaaacgct gttctgtcgt
     8461 tcgtgctcca gacgcgcctg gcgcgcggcg tcattgcccc aaagtaacgc cgtgtggccg
     8521 ttgcgcgcca gcagcatcgc cagcgctgtg ccccaggatc cagcccccaa cacggcgatc
     8581 gtatgtttct tggcggagga catccttatt cttgatctct ggtccggcaa caaaaatcca
     8641 cgttgaccgg attggttgta gcggactgcc gctctctgct ttcgcttctt tccctgagag
     8701 ctaggggtaa tcccaggctc tttagattaa ttaatagccg gctgacttgc ctgtgccgat
     8761 tgcttctgct cgtacaaggc ctcgaaattg atcggggtga tcagcaactg cggaaatcca
     8821 cccttctcga cgaaatcatt cactgcctcg cgtgcaaatg gcagcagcgc ataggcacaa
     8881 ctgatttcaa gggcacgccg taacatttcg ttgttgacgc cgccgatacg gaaaataccc
     8941 gcctgtttta cttccacgag aaaaatattc ttgttctcac gcttggcggt tgcggttatc
     9001 gtcagcacga cttcatacaa tccctgctcc gccgcaagaa tgctatgctc cagacccagc
     9061 tgcacgccga tttcggcgcc ggcattctgc gattgggaaa aggccagggg aacacccggc
     9121 gcttcatagg atacgtcctt gagatatatt ttctcgatac tgaaaacagt gttctgttgt
     9181 tgatcagctt ccgccatctt gccctcgctt taattatcta attctgccct gcaaaaaatc
     9241 acgactttaa caacgtgtcc agctggcccg cgcgctccag ccccgccagt tccgtgtatc
     9301 cgcccacatg cgtctcgccg atgaaaatct gcggcacgct ggtccggccc gcgcgccggg
     9361 tcatttcctc gcggcgcgcc gggtcttcgt ccaccatcac cttctcggcc tgaattccct
     9421 tttgctccag cagtcgctcc gccatgcggc aataggggca gagccggctg ctgtacatca
     9481 gcactttcgc caccattatt tttccaccgg cagattgtcc cgctcccacg cctgcaatcc
     9541 gccagaaaga ttgtataccg tcgggaaacc gtgtttgcgc aggagtacag cgcccttcag
     9601 ggagcggttg ccgctgcggc aactcacgat gaccggcttg ttcttgtatt tgtcgagatc
     9661 gcgcaaccgg tctttcagtg atgacagcgg cagattgatc gcgttgggaa catgaccggc
     9721 gctgaactcc ttgggctcgc acacatccac caccacgccg tgctcgcgat tcagcaactg
     9781 caccgtctgc gcggcgttgg catttttgat gccgtacatt cgctggctga taggaccggt
     9841 ggccagcaga aaaagaataa ccgccagtgc aacgaacaga taccagttat ggataatgaa
     9901 ctgtgtcatt tattcaggat attttgtgtt gtttgatatc agatggtaac gctcaagccg
     9961 gcatcgctct gccggatttc atcaagcgtt gttgattatg gggaagcagc cagctgtttt
    10021 cgataaactg gtcagcgtcg caccgcgctg cagaaggctt cgcgcagcga accgatcaat
    10081 tgcaggattt tcgggtcgct tatccgataa taaaccttgt tggcgtcctt gcgcgcggcc
    10141 agaatgccct tgtcgcgcag gatggaaaga tgctgggaaa tgttgctttg cgaggtcccg
    10201 acctgctcga cgatgtcctg tacactgacc tcggcatgcc cccccagaat gcagaggatt
    10261 ttcaggcgca acgggtgcgc catggctttc agggaacggg aagcaagatg aatatcatct
    10321 tcgttggtaa tcagaacatc ttctactttt tctgcggcgg cgctcatgtc atcaggtctc
    10381 atggatcgct tgtcgcccac gcccctctct ggagccgtgg aatcagcgga tacggctgat
    10441 acgcgaggca aaacattata caatattagt aattttgaaa agactaaatt aaccgatata
    10501 tttgagtcat tgagtatgtc attcactcct aaacccttgg ttctgatcct tttggatgga
    10561 tggggttacc gccaggacac ccagtacaat gccatcgccg ccgcgcgcaa acccgtgtgg
    10621 gacatgctct ggaaacagta tacccacacc ctgatacatg cctcggaagc cgccgtcggc
    10681 ctgccttcca gccagatggg caactcggag gttggtcatc tcaatctcgg cgccgggcgc
    10741 gtcgtgtacc aggaatacac gcgcatcaac cgcgcgatca gcaccggcac cttcttcacc
    10801 aataaaacgc tcaccgacac catcgacctg gcggtcaaga ccgggaaggc
//
LOCUS       DBNGZE010000146         2526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_159429, whole genome shotgun sequence.
ACCESSION   DBNGZE010000146 DBNGZE010000000
VERSION     DBNGZE010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_159429"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="AC16FV_03710"
     CDS             complement(<1..894)
                     /locus_tag="AC16FV_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2849203.1"
                     /translation="MPSVVEIEGLVKRYDSREVVRGIDLSVPAGRCLGVLGPNGAGKT
                     TTLRLLLGQSPVNGGEIHVFGLPIPQSGRAVRARTGVVPQTDNLDPDFTVAENLRVYG
                     RYFGIAAATLDARIRELLEFVDLTDRADARIATLSGGMKRRLTIARALINDPELVVLD
                     EPTTGLDPQVRHMIWGRLRDLRQAGKTLLLTTHYMEEAERLCDELIIMDQGRILEQGT
                     PAALIKKHAEPEVLEVRGEEQLARRALASTGDGRFEAIGDTYYYYTRDAREVVKRLEG
                     MPGLTFLHRPANLEDVFLKLTG"
     gene            1111..1692
                     /locus_tag="AC16FV_03715"
     CDS             1111..1692
                     /locus_tag="AC16FV_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR2849204.1"
                     /translation="MRQKRFSTTRFHRSASLLSRGWQNLSLYWYTADKGPLVFVCVIG
                     LIVATFGYVMHSVFARQDDIRELTCLAFNVYFEARGEPVAGQYAVAEVTMNRVASGRY
                     PDTVCGVVHQENWDPLRKRQVSAFSWTELDERPSLEDETFRQAWEVAEAVYYGRYTPT
                     LAGALHYHATYIKPRWARGNKPVARIGNHVFYK"
     gene            1825..2295
                     /gene="cueR"
                     /locus_tag="AC16FV_03720"
     CDS             1825..2295
                     /gene="cueR"
                     /locus_tag="AC16FV_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HIR2849205.1"
                     /translation="MNIGEAAQASGVSAKMVRHYEEIGLIPRAKRTLAGYRMYSQSDV
                     HTLRFIKQARNLGFSIEQIEQLLALWQDRRRPSRKVKELAQEHIRELEERIRDMQDMK
                     RTLEMLAKHCHGDDRPDCPILEGLEKALPVSGSRRRLDGTRKKKRVTPAPASCH"
     gene            complement(2306..>2526)
                     /locus_tag="AC16FV_03725"
     CDS             complement(2306..>2526)
                     /locus_tag="AC16FV_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR2849206.1"
                     /translation="IGATPGGMGTAFSQTAWLPVLRTLGTRPFFGKNLYVSGAGKIFD
                     AQGRLMDNELRERVRAYIEQFAAFVARA"
BASE COUNT          468 a          780 c          811 g          467 t
ORIGIN      
        1 gccagtcagt ttcaggaaaa catcctccag attcgccggt cggtgcagga aggtcagccc
       61 cggcatgcct tcgaggcgct tgaccacctc gcgcgcgtca cgcgtgtagt agtaataggt
      121 atcgccgatg gcctcgaaac gcccgtcgcc ggtggacgcc agcgcccggc gcgcgagctg
      181 ctcctcgccg cgtacttcga gcacctccgg ttccgcgtgt tttttgatca aagccgctgg
      241 tgtgccctgt tccaggattc gtccctggtc catgatgatc agctcgtcgc acagacgctc
      301 ggcctcttcc atgtaatgcg tggtgagcag cagcgtcttg ccggcctggc gcaggtcgcg
      361 cagccggccc cagatcatgt gccgcacctg cgggtcgagc ccggtggtgg gttcatccag
      421 caccaccagt tcggggtcgt tgatgagggc gcgggcgatg gtgaggcgcc gtttcatgcc
      481 gccagaaagg gtggcgatgc gcgcgtcggc gcggtcggtg agatcgacga attccagcaa
      541 ctcgcggatg cgcgcatcca gcgtcgccgc cgcgatgccg aagtagcggc cgtagacgcg
      601 caggttttcc gcgacggtga aatcgggatc gagattatcc gtttgcggca ccacaccggt
      661 gcgtgcgcgc acggcgcggc cggattgcgg aatcggcagg ccgaagacat ggatctcgcc
      721 gccgtttacc ggcgactgcc caagcagcaa ccgcaacgtg gtggtcttgc ccgcgccgtt
      781 cggcccgagc acgcctaagc agcgcccggc cggcacgctg agatcgatgc cgcgcacgac
      841 ttcacggctg tcgtaacgtt tgaccaggcc ttcgatttcc accacggatg gcatagaggc
      901 cgtactctac cgcccggaac ggggcacggc cagcgcctgt tcttgtcgtt ttcttcgttg
      961 gctcatcaat gcaaatgaaa gcaagccatt gatccgggcg ccggttccgc tggtctgtac
     1021 tgtcttggca gccggggctg cggattctag ctttgatgga taaggtgggg ctgcgcgacg
     1081 ttcggccccg catggagaga taccggggac atgcgccaga aacgtttttc aacaactcgc
     1141 tttcatcgta gcgcctcgct gttgtcgcgc ggctggcaga acctaagcct ttactggtac
     1201 acggcggata agggcccgct tgttttcgtc tgtgtgatcg gcctgatcgt ggccacgttc
     1261 ggttacgtca tgcattcggt tttcgccagg caagacgaca tacgcgaact gacctgtctc
     1321 gccttcaacg tgtacttcga ggcgcgcggc gagcccgtgg ccggccagta cgcggtggcc
     1381 gaggtcacca tgaaccgcgt ggcttcgggc cgctacccgg acacggtctg cggtgtggtg
     1441 caccaggaaa actgggaccc gctgcgcaag cgtcaggtga gcgccttctc ctggaccgaa
     1501 ctggacgaac gaccgtcgct tgaggacgag acgttccgcc aagcctggga agtcgccgaa
     1561 gcggtttatt atggacgtta cacgccgacg ctcgctgggg cgctgcatta ccatgccacg
     1621 tatatcaagc ccagatgggc gcgcggcaac aaacccgtgg cgcggattgg taaccacgtt
     1681 ttttacaaat agcgcagcac cgggcaccgg cccttgaacg tcgcagtgac aacggtgaaa
     1741 ctgccggggc taaaccaccc gttggcggac gccaatgact cgtggcttcc tggcggaagg
     1801 agacagcgca tgaacggtaa taccatgaac atcggcgagg ccgcgcaggc ctcgggcgtg
     1861 tcggcgaaga tggtgcggca ttacgaagag atcggtctca tccccagagc caaacgcacg
     1921 ctcgccgggt accgcatgta ttcgcagagc gacgtgcaca ccctgcgctt catcaagcag
     1981 gcgcgcaacc tgggattttc catcgagcag atcgagcaat tgctggcgct gtggcaggac
     2041 cgccgccgcc cgagccgcaa ggtcaaggag ctggcgcagg agcatatccg ggaactcgag
     2101 gagcgtattc gcgacatgca ggacatgaag cgcacccttg aaatgcttgc gaagcactgc
     2161 cacggcgacg accgtcccga ttgcccgatc ctggaaggac tggaaaaagc gttgccggtg
     2221 tccggctccc gccggcggct cgatggcaca agaaaaaaga agcgcgtcac cccggctcca
     2281 gcttcctgcc attgagcctg gcggtttaag cgcgcgccac gaacgctgcg aactgttcga
     2341 tataggcgcg cacgcgctcg cgcagttcgt tgtccataag acggccctgg gcgtcgaata
     2401 tttttccggc gccagaaacg tagaggtttt ttccaaagaa ggggcgcgtg ccgagcgtgc
     2461 gcaggaccgg cagccacgcc gtttgtgaga atgccgtgcc catgccgccc ggcgtggcgc
     2521 cgatga
//
LOCUS       DBNGZE010000147         8299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_854214, whole genome shotgun sequence.
ACCESSION   DBNGZE010000147 DBNGZE010000000
VERSION     DBNGZE010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_854214"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="AC16FV_03730"
     CDS             <1..1149
                     /locus_tag="AC16FV_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR2849207.1"
                     /translation="YGSCLLGSVNLTKFVEEPFTDGARFNWDKYREVVAVFTRMLDNV
                     VEINGLPLAQQRHEIHHKRRHGMGILGLGSTLTMLRMKYGSPESLEFTERVAREMAEV
                     GWEMGIALAEEKGPAPIMEEEFSVTEEMLVKRPEMRRDGYKVGDKVKGKVLLARYSRY
                     MQQFDSALTDRIAEKGCRFTHHTSIAPTGTISLSLANNASNGIEPSFAHKYARNVIRE
                     GKKSKEKVDVYSYELLAYRTLMNPDADPDATDGKDRLPDYFITADHINPREHVDVQAA
                     AQKWVDSSISKTANVPTDYPYEEFKDIYLYAYDKGLKGCTTFRFNPEAFQGVLVKDKD
                     LENTTYRFQLENGEVIEVKGNEEIEYDGEKHTAANLYDALKEGYYGRF"
     gene            1196..1870
                     /locus_tag="AC16FV_03735"
     CDS             1196..1870
                     /locus_tag="AC16FV_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HIR2849208.1"
                     /translation="MSIKINEKITGYEVVKEEPKRPAESNVVHMHEKLERPEVLVGST
                     YKIKTPLTEHALYVTINDIVLNPDTPHEKRRPFEIFINSKDMAHFQWVVALTRVISAV
                     FRKGGDVTFLVEELHSVFDPRGGYFKKGKYTPSLVADLGDVIERHLKLIGLIQTEEPD
                     EARKQLIAEKRAELKKRAAATETAADETCDFPSDAQLCAKCSTKAVIKMDGCMTCLNC
                     GDSKCG"
     gene            complement(2781..3449)
                     /gene="murU"
                     /locus_tag="AC16FV_03740"
     CDS             complement(2781..3449)
                     /gene="murU"
                     /locus_tag="AC16FV_03740"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HIR2849209.1"
                     /translation="MILAAGRGERMRPLSDTVPKPLLEAGGKPLIVHLIERLVRAGLP
                     DLVINHSHLGKQIEKYLADGSRYGACIRYSHEAGGGLETGGGIFKALPLIDTDPFVVI
                     NGDIWTDFPFERLPTRLAGLAHLVLVDNPPQHPQGDFSLRDGKVHAEDAPRLTFSGIG
                     VYARALFADCRPGKFPLAPLLRAAMARGQVSGEHYAGRWRDVGTPQRLDELDQELRRS
                     SARP"
     gene            complement(3464..4459)
                     /locus_tag="AC16FV_03745"
     CDS             complement(3464..4459)
                     /locus_tag="AC16FV_03745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR2849210.1"
                     /translation="MDKRFEALKDWTGRVLGAGAFDIRPASADASFRRYFRVTLGQRS
                     LIVMDAPPDKGGMRSYVTIARHFHALGLNVPEVLEQDHERGFLLITDLGERLYLGNLN
                     EQTVERLYGDALGALIVLQAGTSTDATDKFLPDYDEALLLREMEIFREWYLGRHLGLK
                     LSAGQNRVLDEVFAQLARSALSQPRVWVHRDYHSRNLMVTPANNPGILDFQDAVLGPV
                     TYDLVSLLRDCYIAWPRERVEDWVKGYHELALQSGIPAGEDDAQFLRWFDLMGVQRHL
                     KATGIFARLNHRDGKPGYLADIPRTLGYVREVSARYLELQPLQALLHELKLPGAA"
     gene            4636..6882
                     /locus_tag="AC16FV_03750"
     CDS             4636..6882
                     /locus_tag="AC16FV_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR2849211.1"
                     /translation="MPRHCLARAVLCLMVGAGLGSTAQAEDAAPCPERPPRPPASARP
                     PATTTENLPAEVYARELRLVNQSVSEFIGDAELRRGEQSLSAERLRYDKNTDLIDATG
                     DVTFKDASGLSYQTRETHLNLGTRTGYAGTGSFRMEDGSARGDAERIDFEGPDHTRFT
                     RVRYTTCAPGQDDWFLKIRELELDTEEDIGTAHHTSVNFYGVPVFYLPYLSFPISDER
                     KSGFLIPRVGHASNRGLEIDTPYYLNLAPDYDDTVTPQYMSKRGLMLQNEFRYLTPRS
                     QGMLQLEGLHDDKLANGDDRAAGIYQHNHVFSPLWFGHVDVRGVSDKKYFDDFRDDLG
                     ITSQTHLPQNAELDYRGPRWNFSARAADYQTIDRSIAPTDRPYARLPQLNLSLNRPTE
                     SNRINYAFESELVSFERDASLTGERLNLSPSLSFPLSNSYAFLTPMIGLRHIAYHLSD
                     VPDANPALTRGVFSLDSGLIFERDSRWSERLFTQTFEPRLYYLYIPGKNQDALPNFDT
                     SVPDFSFYSLFRDNRFVGGDRIGDANQITVAATTRFIDQQDGVERARASLGRIYYLAD
                     REVNLPAATSTADASDIAAEITATLASHWHVRSSAQWNREDSHTQKYSYYFQYNPAKD
                     RIVNLGRRYSRGELEQTDISTEWPFATRWTFRGRSLYSQETNRNLESYAGVQYNACCW
                     ALRLMARRRLEVDTLNSNTATQHFSIMFQLELTGLSKLGHMPDSPLRESMFFSSTPTA
                     TSLGSYTP"
     gene            6996..>8299
                     /locus_tag="AC16FV_03755"
     CDS             6996..>8299
                     /locus_tag="AC16FV_03755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2849212.1"
                     /translation="MHVLGKFATLGMALTGLILSPPSGLAAATPQRATGDVDRIVVVV
                     NDDVITESELNQRLRETRKQLELEKISAPPDTALKKQLLERMVLERLQIQLAAQTGIR
                     IGESDVDRAFETVAQRNKLSAEEFRKALAHKGMDIASYRNQLRDQLTIQQLLEREINN
                     RITVTDSEVDNFLENPQSRADMDVSYQMSHILIGIPEAASPEAIQAARKRAEDVHRQL
                     AEGANFEQTAVSHSQGADALKGGNLGWKKAGELPELFLSALKNLSVVGISDILRSPNG
                     FHILKLNNKRGDTQSEAVTQTHVRHILLRPSEILSLEDARNKLLGLRERIENGEDFAA
                     LARAHSEDTGSAVNGGDLGWANPGQMVPEFEKAMNALKPDQLSQPVKTPFGLHLIQVL
                     GRRSHDVTQERVLAAARQQIHARKADERYEQWARQLRNEAFV"
BASE COUNT         1785 a         2663 c         2396 g         1455 t
ORIGIN      
        1 tacggctcct gcctgctggg ttcggtcaac ctgaccaagt tcgtggaaga gccctttacc
       61 gacggagcgc gcttcaactg ggacaagtat cgcgaggtgg tggcggtgtt cactcgcatg
      121 ctcgacaacg tagtcgagat caacggcctg ccgctcgcgc agcagcgcca cgagatccac
      181 cacaaacgcc gtcacggcat gggtatcctc ggcctgggtt ctacgctcac catgttgcgc
      241 atgaaatacg gctcgcccga gtcgctcgaa ttcaccgagc gcgtggcgcg cgagatggcc
      301 gaggtcggct gggagatggg cattgcgctc gccgaggaaa aaggcccggc ccccatcatg
      361 gaggaggagt tcagtgtcac cgaggagatg ctggtcaagc gcccggaaat gcgccgtgac
      421 ggctacaagg tcggcgacaa ggtcaagggc aaggtgctgc tggcccgcta cagccgctac
      481 atgcagcagt tcgatagcgc actcaccgat cgcatcgccg aaaagggttg ccgcttcacg
      541 caccatacct cgatcgcccc aaccgggacg atctcgctgt cgctggccaa caatgccagc
      601 aacggcatcg agccctcgtt tgcccacaag tacgcacgca acgtgattcg tgagggcaag
      661 aagagcaagg agaaggtgga cgtgtattcc tacgagctgc tcgcttaccg cacgctgatg
      721 aatcccgacg ccgatccgga tgccaccgac ggcaaggacc ggctgccgga ctacttcatc
      781 accgccgatc acatcaatcc gcgcgagcac gtggacgtgc aggccgcggc gcagaaatgg
      841 gtggactcct ccatctccaa gaccgccaac gtccccacgg actatcccta cgaagagttc
      901 aaggatattt acctgtatgc ctacgacaag ggcctgaagg gctgcaccac cttccgcttc
      961 aaccccgagg ccttccaggg cgtgctggtc aaggacaagg atctcgagaa caccacctac
     1021 cgtttccagc tggagaacgg cgaggtcatc gaggtcaagg gcaacgagga gatcgagtac
     1081 gacggcgaga agcacaccgc cgccaacctg tacgacgccc tcaaggaagg ctattacggc
     1141 cggttctgaa cacgggataa ctgatagata taacggtaga gattaaggag ctgtcatgag
     1201 catcaagatc aacgaaaaaa tcaccgggta cgaagtcgtc aaggaggagc ccaagcgccc
     1261 ggccgagagc aacgtcgtgc acatgcacga gaagctcgag cggcccgagg tgctggtcgg
     1321 ctccacctac aagatcaaga cccccctcac ggagcacgcc ctgtacgtca ccatcaacga
     1381 catcgtgctc aacccggaca cgccgcacga gaagcgtcgc cccttcgaga tcttcatcaa
     1441 ctccaaggac atggcccact tccagtgggt ggtggccctg acccgggtca tctcggcggt
     1501 gttccgcaag gggggtgacg tcaccttcct ggtggaggag ctgcactcgg tgttcgaccc
     1561 gcgcggcggc tacttcaaga agggcaagta caccccttcg ctcgtcgccg acctgggcga
     1621 tgtcatcgag cgccacctga aactgatcgg cctgatccag accgaggaac cggacgaggc
     1681 gcgcaagcag ttgatcgccg aaaagcgcgc cgagctgaaa aagcgcgccg cagcaacgga
     1741 aaccgccgcc gacgagacct gcgacttccc gtccgatgcg caactctgcg ccaagtgttc
     1801 caccaaggcc gtcatcaaga tggatggatg catgacctgt ctcaactgcg gcgactcgaa
     1861 gtgcggatga catcgttaac atgaagagcg cgacaagact tcgtcataag gcaagacgaa
     1921 cgagcgtagc gagtagggct tcattgtccg cgcaaaaaat ttccggacaa agtgtttgca
     1981 attttttttt cattggatta atgtcttgac gcatcaggac gatgtgctaa acatgaagtg
     2041 aacttgtgcg taaatgtagc cctactcgct acgctcgttc gtcttgatac atgaggtgtc
     2101 actgcccgga tgacgggatg ctgcgagaga agatgaacat catcgcagcg ccgggcggaa
     2161 caaggatcaa ccaagagtta atggatcgat tgcgaagctc gacaaccggt agcaaatgtg
     2221 tcggtacccc cctgcagcag aatctggctg ccaggtcctg ttcttcccgc tctcctcgtc
     2281 tgcttctgtc cgccgggcat gaccggcgca taaccctgtc ccgaatccat gaaaagacag
     2341 taagcgactg tcttttgcac gtggcaacgc gcagtagcgt cgggggtttt tggcccctgg
     2401 cttccctgca agtcccttaa ccacaaacca aaggagctca tcatggcagc caagaaaaag
     2461 gccaagaaaa aagcagctaa gaaaaagaaa taacgcgaca ggcgagggaa tttcggatcg
     2521 tagcgccttc cacgccgagt gcgggaagtt gtcgctgctc tggatgacct tgacgccttc
     2581 gttagtgata agccggtaac atccgcctgc gtcaaggttg gcgaggtgtt accggttttt
     2641 tatcgcccgt gcgaacgcgc tatgggcatt accactgatc gacagcctga tggtcctggc
     2701 agcgacgttc ccgcttttct attttcgccg caatgactct ccctgcccac cggagataat
     2761 tttcaacgaa aaacagtggc ttagggcctg gcggaggagc gacgtagttc ctgatccagc
     2821 tcatcgaggc gttgtggcgt accgacgtcg cgccagcgtc cggcgtagtg ctcaccgctc
     2881 acctgtccgc gcgccatggc ggcgcgcaac aagggcgcca gcggaaactt tccggggcga
     2941 cagtccgcaa acagagcgcg cgcatagaca ccgatcccgc tgaaggtcag ccgcggcgca
     3001 tcctccgcat ggaccttgcc atcacgcagt gaaaaatccc cctgcggatg ctgcgggggg
     3061 ttgtcgacca gcaccagatg cgccagaccc gcaagacggg tcggcaagcg ttcaaaggga
     3121 aaatccgtcc agatatcccc gttgatcacc acgaacggat cggtgtcgat caggggcaac
     3181 gccttgaaga tgccgccgcc ggtttcaagg ccgccgccgg cttcatgcga atagcggatg
     3241 cacgcgccat agcgactgcc atccgcgaga tatttctcga tctgtttgcc cagatgcgag
     3301 tgattgatca cgagatcggg caggcccgcg cgcaccagac gttctatgag gtgcacgatc
     3361 agcggcttgc cgcctgcctc gagcaggggc ttgggaacgg tgtccgacag cggtcgcatg
     3421 cgctcgcccc ggccggccgc gaggatcatg accttgggtc gcgtcacgcc gcccccggca
     3481 acttgagttc atgcaacaag gcctgcaacg gttgcagttc cagatagcgg gcgctcacct
     3541 cgcgcacata accgagggtg cgcgggatgt cggcgagata accgggcttg ccgtcgcgat
     3601 ggttcaggcg cgcgaagata ccggtggcct tgagatgacg ctgcactccc atcagatcga
     3661 accagcgcag gaattgcgca tcatcctcgc ccgccggtat gccggactgg agcgccagct
     3721 cgtgatagcc cttgacccag tcttccaccc gctcgcgcgg ccaggcgatg tagcaatcgc
     3781 gcaacaagga cacgagatcg taggtcaccg gccccagcac ggcgtcctgg aaatcgagaa
     3841 tgcccgggtt gttggcaggc gtgaccatca ggttgcgcga atggtagtcg cgatgcaccc
     3901 acacccgcgg ttgggagagg gccgagcgcg ccagttgcgc aaagacctcg tcgagcaccc
     3961 ggttctggcc ggcgctgagc ttgagcccca gatgacgtcc cagataccac tcgcggaaga
     4021 tctccatttc gcgcagcagc agtgcttcgt catagtccgg caggaattta tcggtggcat
     4081 cggtcgaggt accggcctgc agcacaatca gcgcgcccag cgcatcgcca taaagccgct
     4141 ccactgtctg ttcgttcagg tttcccagat agagccgctc gcccagatcg gtgattagca
     4201 ggaaaccccg ctcgtggtcc tgctccagca cttccggcac gttcagcccc agcgcatgga
     4261 aatggcgcgc aatggtcacg taggaacgca tgccgccctt gtccggcggc gcgtccatga
     4321 cgatcaggct gcgctggccc agcgtgacgc ggaaataacg gcgaaagctc gcgtccgccg
     4381 aggccggacg gatatcgaat gcgcccgcgc ccaaaacccg gccggtccag tccttgagtg
     4441 cctcgaaacg tttatccaaa agtggtccgg taaataaaac ccgtgcgagc gggaaaacgc
     4501 cttgatggag ccgacatagt atgcaatttt atgcccacca tcgaaaatgg cgccctattt
     4561 cattcgtctg gccatcaaaa aggtcgccct acttcgttcg tctggacata ataaatcaat
     4621 gtcatcctcg ttcccatgcc tcgccattgt ctagctcgcg ccgtcctgtg cctgatggtt
     4681 ggcgcgggcc tgggttcgac agcgcaagcc gaggacgcag caccctgccc ggagcgcccg
     4741 cctcggccgc ccgcctcggc acgaccaccc gccacgacga cggagaatct gccggcggag
     4801 gtgtacgccc gggaactgcg cctggtaaac cagagcgtca gcgaattcat cggcgacgcg
     4861 gaactgcggc ggggtgagca gtccctcagc gccgagcgcc tgcgctatga caagaacacc
     4921 gatctgatcg acgccaccgg cgacgtcacg ttcaaggacg cctccggcct gagctaccag
     4981 acccgggaaa cgcacctcaa tctgggaacg cgcaccggct atgccggaac cggcagtttt
     5041 cgcatggagg atggcagcgc ccgtggcgat gccgagcgca tcgacttcga aggcccggac
     5101 cacacgcgct tcacgcgggt gcgctacacc acctgcgcgc cgggtcagga cgactggttt
     5161 ctcaagatca gggagctcga actcgacact gaagaggaca tcggcaccgc ccatcacacg
     5221 tccgtcaact tctatggcgt gccggtcttt tacctgccgt atctcagttt tccgatttcc
     5281 gatgagcgca aatccggctt tctgatacct cgcgtgggtc acgccagcaa tcgggggctc
     5341 gaaattgaca cgccttacta tctcaatctc gctcccgatt acgacgacac cgtgacgccc
     5401 cagtacatgt ccaagcgcgg cctgatgctg caaaacgagt tccgttatct caccccgcgc
     5461 tcccagggca tgttgcaact ggagggcctg catgacgaca aactggccaa cggtgatgat
     5521 cgcgcggcgg ggatttatca gcacaaccat gtcttcagtc ccttgtggtt cggccacgtg
     5581 gatgtgcgcg gcgtatcgga taaaaaatac tttgacgatt tccgcgacga tctcggcatc
     5641 accagccaga cccacctgcc gcagaatgcc gagctggatt atcgcggccc gcgatggaat
     5701 ttcagcgccc gcgccgcgga ctaccagacc atcgaccgct ccattgcgcc cacggatcgc
     5761 ccctacgcgc ggcttcccca gctcaacctt tcgctcaacc gtccgaccga atccaatcgg
     5821 atcaattacg ctttcgagag cgaactggtg agtttcgagc gcgatgccag ccttaccggc
     5881 gaacgtctga atctgagtcc gtcgctgagt ttcccgttat ccaacagcta tgcctttctg
     5941 acccccatga tcggcctgcg acatatcgcc taccaccttt ccgacgtgcc ggatgccaac
     6001 ccggcgctga cgcggggtgt attcagcctc gacagcggac tcatcttcga gcgcgactcg
     6061 cgctggagcg agcgcctgtt cacgcagacg ttcgagccgc gcctgtatta cctctacata
     6121 cccggcaaaa atcaggatgc cttgccgaac ttcgatacca gcgtgcccga cttcagtttc
     6181 tacagcctgt tccgcgacaa ccgtttcgtc ggcggggacc gcatcggaga cgccaaccag
     6241 atcaccgtcg ccgccaccac gcgttttatc gaccagcagg atggcgtcga gcgcgcacgc
     6301 gccagcctcg gaaggattta ttaccttgcc gacagggagg tcaacctgcc ggcggcaacc
     6361 agcaccgccg atgcctcgga tatcgcggct gagatcacgg cgacgctcgc cagccactgg
     6421 catgtgcgca gcagcgccca atggaaccgc gaggacagcc acacgcagaa atacagctat
     6481 tactttcagt acaatccggc gaaagaccgc atcgtgaacc tcggtcggcg ctactcacgc
     6541 ggtgaactgg aacaaaccga catctccacg gaatggccct tcgccacgcg ttggaccttc
     6601 cgtggccgct cgctttactc ccaggagacc aaccgcaact tggagagcta tgccggcgtg
     6661 caatacaacg cctgttgctg ggcgctgcgg ctgatggcga ggcggcggct cgaggtggat
     6721 acgctgaaca gcaataccgc cacccaacac ttttccatca tgtttcaact cgagctgacc
     6781 ggcctgtcca aactcgggca catgccggac agcccactgc gcgaaagcat gtttttttct
     6841 tctactccga cggccacgtc cctggggtcc tacacaccct agttgcatcc tcggctccgt
     6901 cagcgcgcca tccttggcgc acttttcatg ttatcctagc gtcgctcgaa ctccggcgcc
     6961 tgcgcgctgt ccacggaata cccggataca aattcatgca tgttctcgga aaattcgcca
     7021 cgctcgggat ggcgctgaca ggattgatcc tgtcgccgcc gtccggcctc gcggcggcca
     7081 cgccgcagcg cgccaccggc gatgtcgatc gcatcgtggt ggtcgtcaac gacgacgtca
     7141 tcaccgaaag cgaactcaac cagcggctgc gcgaaacccg caagcagctg gaactggaga
     7201 aaattagcgc gccgccggat accgcgctca aaaagcagct gctcgaaaga atggtgctgg
     7261 agcgcctgca gatacagctg gccgcccaga ccggcattcg catcggcgaa tccgacgtgg
     7321 accgcgcctt cgaaaccgtg gcgcagcgca acaaactcag cgcggaggaa tttcgcaagg
     7381 ccctggcgca caagggcatg gacatcgcgt cgtaccgcaa ccagttgcgc gaccagctga
     7441 ccatccagca gctgctcgag cgcgaaatca acaaccgcat caccgtcacc gacagcgagg
     7501 tcgataactt cctcgaaaat ccgcagagcc gcgccgacat ggacgtgtcc tatcagatgt
     7561 cgcatatcct gatcggcatt cccgaagcgg cctcgcccga ggcgatccag gcggcccgca
     7621 agcgcgccga agacgtgcac cgtcagctcg ccgagggcgc caatttcgag cagaccgccg
     7681 tgagccactc ccagggcgcg gacgccctca agggcggcaa tctgggctgg aagaaggcgg
     7741 gcgagctgcc ggaattgttc ctgagcgcgc tgaaaaacct gtccgtcgtc ggcatcagcg
     7801 acattctgcg cagccccaac ggcttccata tcctgaaact gaacaacaaa cgcggcgata
     7861 ctcagtcgga ggcagtgacc cagacgcacg tgcgccatat tctgctgcgt cccagtgaaa
     7921 tcctgtcact ggaggatgcc cgcaacaaac tgctgggcct gcgcgagcgc atcgagaacg
     7981 gcgaggattt cgccgcgctc gcccgcgccc attccgagga caccggttcg gccgttaacg
     8041 gcggcgactt gggctgggcc aaccccggcc agatggtgcc ggaattcgag aaggccatga
     8101 acgcgctcaa gccggaccag ttgagccagc ccgtgaaaac ccctttcggc ctgcacctca
     8161 tccaggtgct tggccggcgc agccacgacg tgacccagga acgcgtgctg gccgcggcgc
     8221 gccagcagat ccacgcacgc aaggcggacg aacgctacga gcaatgggcg cgccagctgc
     8281 gcaacgaggc gttcgtcga
//
LOCUS       DBNGZE010000148        10538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1548892, whole genome shotgun sequence.
ACCESSION   DBNGZE010000148 DBNGZE010000000
VERSION     DBNGZE010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1548892"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16FV_03760"
     CDS             complement(<1..485)
                     /locus_tag="AC16FV_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849213.1"
                     /translation="MKLRRSLRQRVALGMALLTLAIVGAHSVATYSITHEQEEDSIGR
                     IVNEELEYLVTQYRRDLSVPVLPHHPNLSVYIAPTAAARAALPAPLRELSVGLHDGVR
                     IGGAAFHVGVRASGETRFYLVYDIAYHEARMRKFRWLLALGVAATAALSATLGYWLAG
                     WL"
     gene            complement(475..1158)
                     /locus_tag="AC16FV_03765"
     CDS             complement(475..1158)
                     /locus_tag="AC16FV_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2849214.1"
                     /translation="MRILIVEDDRDIASNLHDYLIARGHLADRAADGVTGLRLALTQD
                     YDTILLDLGLPGIDGLAFTRKLREEAARDTPILVLTARDTLEDKLAGFEHGVDDYMVK
                     PFALREVEARLAALDKRRRGTVTRHTLVVGDLAFDTGTLEVTRAGVSIRLPPKCLRLL
                     ELLMQQPNHVFSRAELETAVWGGTLESSDTLRAHMHMLRRALTRPARPDPIETVHGLG
                     YRLVTRHET"
     gene            1246..3312
                     /locus_tag="AC16FV_03770"
     CDS             1246..3312
                     /locus_tag="AC16FV_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR2849215.1"
                     /translation="MNAPAHTNRLIHETSPYLQQHAHNPVDWYPWGAEALDKARRENK
                     PILVSIGYSACHWCHVMEHESFEDEEVARVMNELFVCIKVDREERPDLDKIYQTAHQI
                     LAQRPGGWPLNMFLTPDDQMPFFGGTYFPKTSKYGMPAFTDLLRHVAEVYDKKHDAVR
                     EQNHSLRDIFGRLQPPGPAPGVHVGPEVLEQAHTELQRQFDDRYGGFGRAPKVPHPTS
                     LELCLHRWARSRRDGAEDKDALHMARHTLAAMAHGGLYDQIGGGFCRYSVDERWEIPH
                     FEKMLYDNAQLLVLYADAWLATGESLFRRIAIETAEWVMREMQSPEGGYYSALDADSE
                     GHEGKFYVWTVDEIRSLLTPEEWDVVERRFGLQGTPNFEGQWHLNVRATYDAIADQFQ
                     RPESEVIELLDSARGKLFATREQRVRPGRDEKILTSWNGLMIQAMAHAGRMLEQPDFT
                     ASAERAMDFIHAQLWRNQRLLATHKDGKSHLNAYLDDHVFLIAGALELLQARFRRADL
                     DFAIALADNVLEHFEDESHGGFYFTGDDHEKLVYRPKPVSDDAIPSGNGVAARALGQL
                     GHLLGDLHYLHAAERTLEALYSGMQQQPSAHGALLLALEEQLSPPQTIVLRGTPEAMQ
                     PWQTVAGRHYHPHRLVLAIPDDAGNLPGILAERVTRNGVTAYVCAGHACSAPVTDLKD
                     FEAALN"
     gene            complement(3410..4426)
                     /locus_tag="AC16FV_03775"
     CDS             complement(3410..4426)
                     /locus_tag="AC16FV_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR2849216.1"
                     /translation="MKLSAIIKAGLAGVLALVASVVFSAAHAATINVTRCVGSLNKTL
                     SGQTVTFWGFGTGGMMCTGALPGAAIEVGVYPAGSGMSDTLNLTLTINGMTPQEAAPY
                     SGHTIHMHGADVPTSEDGVPETYNDTNGDTYTWTPTTEMAGSYLYHCHVHTVKHLEMG
                     MYGALIARPKNTSGAWLNQLTHNTATAFDYVQNYVLGTVDPAYHTATGDSTVFADYNP
                     VYFLISGNQGLTTSAPAITLAAAVNKKVALRLLGLHSANSTFAVKDGSGSAKSFTVYT
                     QDGRELSTPQTVTSLDVSPGQRFDIVFTTPATTGTWYPQITYKNLRNGTAYTNGTVYG
                     RVTF"
     gene            complement(4449..5372)
                     /locus_tag="AC16FV_03780"
     CDS             complement(4449..5372)
                     /locus_tag="AC16FV_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR2849217.1"
                     /translation="MKWHLVVRVVAACLLFGLASSAGAATRNYTLYITAGTLTINGSG
                     GASMAAWGYTEVAGTPKFPGPTLTANEGDTVNVTVVNNHNINHNFLVQGVTTDTTAIA
                     PGGNRVYSFTASTAGSYLYYDTLNSSINREMGLYGMLWVGPADGSNKAWTNGPSYSFQ
                     RLWVVGEMDKARWNDVAGGGGTVNTSVYKPNYFLINGQGGFDGMNNTATTIDGGVGQT
                     ALVRIVNGGQFAQSLHFHGNHVQVLTINGVRQSSPYKKVDVINVPPLGTADVLFVLNQ
                     LGSYPMHNHTAQMETANGVYLNGVATMIIMR"
     gene            5714..6103
                     /locus_tag="AC16FV_03785"
     CDS             5714..6103
                     /locus_tag="AC16FV_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2849218.1"
                     /translation="MAKTLADFVREARARVREISVDEFDEMIENHDDLLIVDVREAGE
                     FQRGHIPGALLLPHGLLEGTVDDTRRLQLEQLFGGYQKTFVLCSRDGSRSAVAARALQ
                     QIGFEKVYSLAGGIDRWQAQGFALVTE"
     gene            6244..6594
                     /locus_tag="AC16FV_03790"
     CDS             6244..6594
                     /locus_tag="AC16FV_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849219.1"
                     /translation="MEPQSSTKAVSTNVVSTTGTGMVAGVRQMFRATRMLHEREKSRI
                     REEIAQIPGLMALLMKPRNGLKWTRTERAELRTQLRRLSRLSIYLALATLPFTSLTLP
                     LVAWWLDQRQQKRT"
     gene            complement(6768..7343)
                     /locus_tag="AC16FV_03795"
     CDS             complement(6768..7343)
                     /locus_tag="AC16FV_03795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR2849220.1"
                     /translation="MLDVIHGFFNHPVARKFFLKLRYVLFLAVLVLLARYADVSRLLV
                     AFLVSLFGEAIQLWSFASLVKNEQLTARGPYVMVRNPMYLGRFFLILGVMLLFGNYYL
                     IIAYTVFYYFYMVNRVGREEKRLAELLGQPYSDYRARVNRFLPSFGRLSDPAVRFFNW
                     SVLYRNNGHWNLLAMLVFYGILAGYLVYFRS"
     gene            7559..9364
                     /locus_tag="AC16FV_03800"
     CDS             7559..9364
                     /locus_tag="AC16FV_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR2849221.1"
                     /translation="MLDYQAPQCNVFWVRFFPFLRWWPRVTRETLKADLIAGFTGAVI
                     VLPQGVAFAMIAGLPPVYGLYTAMVPPIIAGLFGSSRHLISGPTTAISIVVFATITKF
                     AEPGSAKFISMALTLTFLAGVYQFVLGLARMGALVNFVSHSVVVGFTAGAAILIATSQ
                     LKHFLGINIPSGESFLHTWQDIFRQIGDIDPYVTAIAAVTLIAGILFMRFKPRWPGML
                     FAMIIGSGLAALLAYETKGIRLVGEIPAQLPPFSMPDLSLKSLKALGSGALAIAMLGL
                     VEAVSIAKSVALKSGQRINGNQEFIGQGLANMVGSFFSSYASSGSFTRSGINYTAGAI
                     TPMAAIFAAISLALIVLAVAPLAAHLPIAAMAGILLIVAYRLIDFHHIANILRTSKRE
                     TAVLLTTFLATLFVELEFAIYVGVMLSLVIYLMRTSRPGIVVRTPDPHSAGRHMVTDP
                     KLPECPQLKIVRVDGSLFFGAVDHVQGEFQRFQAENPGQKHLMIIGNGINFVDIAGAE
                     MLVQEARRRRNLGGGLYLSKVKEEACRILKRGGFVDHLGDEHIFVTKADAIQTIFAKL
                     DRSICERCDKRIFLECQKIEYKGDKPLPADKQAAA"
     gene            complement(9475..9861)
                     /locus_tag="AC16FV_03805"
     CDS             complement(9475..9861)
                     /locus_tag="AC16FV_03805"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR2849222.1"
                     /translation="MRRLRTKGWKGLFDLGRVWRAAGYSAAGFRAALKNEAAFRQELA
                     LFVVLAPLGFWLGRDGVERSLLVGSLMLVLLVELLNSAIEAVVNRVGRDPHELSGQAK
                     DIASAAVFVSLVLVVLVWTLVLADRF"
     gene            complement(9893..>10538)
                     /locus_tag="AC16FV_03810"
     CDS             complement(9893..>10538)
                     /locus_tag="AC16FV_03810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111236.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR2849223.1"
                     /translation="HGGGGPGAGPVAASSRLEPFLPVPIVGKVQDKYLWETEKTRPQT
                     IGRLSANMGNAGVLLRAYVYARLLGREGMHRVAEFSTLNANYIMAELAKRGFDMAFPK
                     RRATHEFVLTFKKLKDETGVSAMDVAKRLLDKGFHAPTTYFPLLVPECHLIEPTETEA
                     REELDRFIAAMEEIMREVRSSPDVVKGAPHTLPVKRLDDVRAARELDLTWKQAG"
BASE COUNT         2078 a         3293 c         3125 g         2042 t
ORIGIN      
        1 agccacccgg cgagccagta gccgagcgtg gcggaaagcg ccgcggtggc ggcgacaccg
       61 agggcaagca gccagcggaa cttgcgcatg cgcgcctcgt ggtacgcaat atcgtagaca
      121 agataaaacc gcgtctcgcc cgacgcgcgc actcccacat ggaacgccgc gccgccgatg
      181 cgcaccccgt cgtgaaggcc gacagaaagc tcgcgcagcg gcgccggcaa tgccgcgcgc
      241 gcggccgccg tgggcgctat atagacgctg agattcgggt ggtgcggcag cacagggacg
      301 ctcaggtcgc gccggtactg cgtcaccagg tactcgagtt cctcgttcac gatccggccg
      361 atggagtctt cctcctgctc gtgcgtgatc gaataggtcg caacgctgtg cgcgcccacg
      421 atcgcgagcg tcagcagcgc catgccgagc gccacgcgct gccgcaggct ccgcctaagt
      481 ttcatgccgc gtcaccaggc ggtagccaag accgtgcacg gtctcgatcg gatccggccg
      541 ggccggccgc gtgagcgcgc gccgtaacat gtgcatgtgc gcgcgcaagg tgtcgctgct
      601 ttcgagcgtg ccgccccaga ccgcggtctc gagttccgcg cggctgaaca catggttcgg
      661 ctgctgcatc agcagttcga gcaggcgcag gcacttcggt ggaagccgga tggatacgcc
      721 ggcgcgcgtc acctccagcg tacccgtgtc gaacgccaga tcgcccacga cgagcgtgtg
      781 ccgcgtcacc gtgccgcgtc ggcgtttgtc gagcgccgcc agtcgcgcct cgacctcgcg
      841 caatgcgaat ggcttgacca tgtagtcgtc gacgccgtgt tcgaagccgg cgagtttgtc
      901 ctcgagcgtg tcgcgggcgg tgagcacgag gatgggcgtg tcgcgggcgg cctcctcgcg
      961 cagcttgcgg gtgaacgcca gcccgtcgat ccccggcaga ccgagatcga gcaggatcgt
     1021 atcgtagtcc tgggtaagcg ccaggcgcag gccggtaacg ccgtcggccg cgcggtccgc
     1081 gaggtgtcca cgcgcgatga ggtagtcatg aagattcgag gcgatgtcac ggtcgtcctc
     1141 gacaatcaga atgcgcacgc aaaaccccca acgtttgaag atgtactata agacagacca
     1201 tcagggttgc gatatagttt aaattaattc tctccgcggc cttccatgaa cgcacccgcc
     1261 cataccaacc gcctcatcca cgaaaccagc ccctacctcc agcagcacgc gcacaatccg
     1321 gtggactggt atccgtgggg cgcggaagcg ctcgacaagg cgcggcgcga gaacaagccg
     1381 atcctggtct cgatcggcta ctcggcctgt cactggtgcc atgtgatgga acacgaatcg
     1441 ttcgaggatg aagaggtcgc gcgcgtgatg aacgagcttt tcgtatgcat caaggtggat
     1501 cgcgaggagc gtcccgatct cgacaagatt tatcagaccg cgcatcagat tctggcgcag
     1561 cgccccggcg gctggccgct caacatgttt ctcacgcccg acgaccagat gccgtttttc
     1621 ggcgggacgt attttcccaa gacatccaaa tacggcatgc cggctttcac cgatctgctg
     1681 cgccacgtgg ccgaggtcta cgataaaaaa cacgatgccg tgcgggagca gaaccattca
     1741 ctgcgggaca tcttcgggcg cttgcagccg ccggggcccg cgcccggcgt gcatgtcgga
     1801 ccggaggtgc tggagcaggc ccataccgaa ttgcagcgcc agtttgatga ccgctacggt
     1861 ggctttggcc gggcgcccaa ggtcccgcac cccacgtcgc tcgaattatg cctgcaccgc
     1921 tgggcgcgca gccgccgcga cggcgcggaa gacaaggacg ccctgcacat ggcgcggcac
     1981 acgctcgcgg ccatggccca cggcgggctt tacgaccaga tcggcggcgg attctgccgc
     2041 tactcggtgg acgagcgctg ggagattcca catttcgaaa aaatgctgta cgacaacgcg
     2101 cagttgctgg tgctctatgc cgatgcctgg ctcgccaccg gcgagtcgct gttccggcgc
     2161 atcgccatcg aaaccgccga atgggtgatg cgggaaatgc aatcacccga aggcggctac
     2221 tactcggcgc tcgatgccga ctccgaggga cacgaaggca agttctatgt ctggacggtg
     2281 gatgaaatac ggtcgctgtt gacacccgag gaatgggatg tggtcgaacg ccgcttcggc
     2341 ctgcaaggca cgcccaattt cgagggtcag tggcacctca acgtgcgcgc cacctacgat
     2401 gccatcgccg accagtttca gcgcccggaa tcggaagtca tcgaactgct cgattcggcg
     2461 cgcggcaaac tgttcgccac gcgcgaacag cgcgtgcgac cggggcggga cgagaaaatc
     2521 ctcacatcct ggaacggact catgattcag gccatggcgc atgcgggccg gatgctggag
     2581 cagccggatt tcaccgcctc cgccgaacgc gccatggatt tcatccacgc acagctgtgg
     2641 cgcaatcagc gcctgctcgc cacgcacaag gacggcaaat cgcatctcaa cgcctatctg
     2701 gacgatcatg tgtttctgat cgcgggcgcg ctcgaattgc tgcaagcgcg cttccgccgc
     2761 gccgatctgg attttgccat cgcgctcgcg gacaacgtgc tggaacattt cgaggatgaa
     2821 agccacggcg gtttttattt caccggcgac gatcacgaaa aactcgtcta ccgccccaag
     2881 ccggtgtcgg acgacgccat tccttccggc aacggcgtcg ctgcgcgggc gctgggacaa
     2941 ctcggccatc tgctcggcga ccttcattat ctgcatgccg ccgagcgcac tctggaggcg
     3001 ctgtattcgg gcatgcagca acagccttcc gcgcacggcg cactgttgct ggcactggag
     3061 gaacaattat cgccgccgca gaccattgtt ctgcgcggca cgcccgaggc gatgcagcct
     3121 tggcaaaccg tcgccggccg gcattatcat ccgcaccgtc tggtgttggc gatcccggac
     3181 gacgcgggaa atttgcccgg catactggcc gaacgcgtaa ccagaaacgg cgtcacggcc
     3241 tatgtctgcg ccggtcacgc ctgttcagcg cccgtcaccg atctcaagga tttcgaagcg
     3301 gctttgaatt gaaggggccc gcccccagtc gcggcattgc tccatcccgg agggcaaaaa
     3361 aaacgggccg ccgtgaggcg gcccgtttga ctgttgcttt gcaataaaac tagaacgtca
     3421 cccggccata caccgtccca ttggtatagg ctgtcccatt gcgcaggttc ttgtaggtga
     3481 tctgcggata ccacgtgccg gtggtcgcgg gtgtagtgaa gacgatgtca aagcgctgtc
     3541 ccggggacac atcgagactc gtcaccgtct gcggcgtgga cagctcgcgc ccgtcctgcg
     3601 tatataccgt gaacgacttg gcgctgccgg acccgtcctt gacggcgaag gtgctgttcg
     3661 ccgagtgcag cccgagcagg cgcagcgcca cctttttgtt taccgccgcc gcgagcgtga
     3721 tggcgggcgc ggaggtggtc aggccctggt tgccgctgat caggaagtac accgggttgt
     3781 agtcggcaaa caccgtggag tcaccggtgg cggtgtgata cgccgggtcc acggtaccga
     3841 gcacgtagtt ctgcacgtag tcgaacgccg tcgccgtatt gtgcgtaagc tgattgagcc
     3901 aggcccccga ggtgttcttg gggcgtgcga tcagcgcgcc gtacatgccc atctcgaggt
     3961 gcttcaccgt gtgcacgtgg cagtggtaca gatagctgcc ggccatctcc gttgtcggcg
     4021 tccaggtgta ggtgtcgccg ttggtatcgt tgtaggtctc cggtacaccg tcctcgctgg
     4081 tcggcacatc cgcgccgtgc atgtggatag tgtgaccgct gtacggcgcg gcctcctgcg
     4141 gcgtcatccc gttgatggtt agcgtgaggt tcaaggtatc gctcatgccc gaccccgccg
     4201 ggtagacacc cacttcgatg gctgcgcccg gcagggcgcc ggtgcacatc atgccgccgg
     4261 tgccaaagcc ccagaaggtg acggtctgac cgctcagagt cttgttcaga ctgcccacac
     4321 accgtgtgac gttgatggtg gcggcatgcg ccgcgctgaa cacaacactg gcgacgagcg
     4381 ccagcacgcc ggcgagaccg gccttgatga ttgctgaaag tttcatgact tgatttctcc
     4441 tccgtaagct agcgcatgat gatcatggtg gcgacgccgt tcagatacac gccgttggcg
     4501 gtctccatct gcgccgtgtg attgtgcatc gggtaactgc cgagctggtt cagaacgaac
     4561 agcacgtcgg ccgtcccgag cggaggaacg ttgatcacgt ccaccttctt atagggcgaa
     4621 ctctgacgaa cgccgttgat cgtcagcacc tgcacgtggt tgccgtggaa gtgcagcgac
     4681 tgcgcgaact gcccgccgtt gacgatgcgc accagcgccg tctggcccac gccgccgtca
     4741 atggtcgtgg cggtgttgtt catgccgtcg aacccgccct ggccgttgat caggaagtag
     4801 ttcggcttgt acaccgaggt attgaccgtg ccgccaccgc cggcgacatc gttccaccgg
     4861 gctttatcca tctcgcccac gacccacagg cgctggaagc tgtacgacgg gccgttggtc
     4921 caggccttgt tgctgccgtc ggccggaccc acccagagca tgccgtacaa tcccatctcg
     4981 cggttgatgc tgctgttgag cgtgtcgtag tagagatagc tgcccgccgt gctggccgtg
     5041 aacgagtaga cgcggttgcc tcccggcgcg atggcggtgg tgtcggtcgt gacaccctgg
     5101 acaagaaagt tgtgattgat gttgtgattg ttgacgaccg tgacgttgac ggtatcgcct
     5161 tcattcgccg tcaatgtcgg tccggggaat ttcggggtac ccgcgacttc cgtgtaaccc
     5221 caggcggcca tcgaagcgcc gccgctcccg ttaatcgtca acgttcccgc ggttatataa
     5281 agagtgtaat tgcgtgtagc agcgcccgcc gacgaggcca gaccgaacag aagacacgct
     5341 gcaacaaccc tgacaacaag atgccatttc atgaatttcc tccatgtggg aaagaacacc
     5401 gagtagtagt gataatgggt ttatgctgtg ttgctgatgc tcccctgtgc caagttactg
     5461 aaatgccgga ggtaaagaac tcccttgaat ccccccgtta tctatatgta ataaaaaata
     5521 ttcacagaat cttcacaaat cgtttacata ctcttcctgt gtgtgattta tgtcaatttt
     5581 tttatatgta tatagttaat atttatcagt attagaatct gttgatatgc ttttatattt
     5641 tgaatcgcgc tgacttaggc gtgcctccga attattctct ttaaaataac cgagggtccg
     5701 ggtaaggatt cacatggcga aaactctggc ggattttgtg cgcgaggcgc gtgcccgcgt
     5761 gcgcgagatc agcgtcgatg aattcgacga gatgatcgag aaccacgacg acctgttgat
     5821 cgtggacgtg cgcgaggctg gagaattcca gcgcggacat attccgggcg cgctgctgct
     5881 cccgcacggc ctgctggaag gcacggtgga cgacaccagg cgtttgcaac tggagcagct
     5941 gttcggcgga taccaaaaga cgtttgtgct ctgcagtcgg gacggttcgc gcagcgccgt
     6001 cgcggcgcgc gcgctgcaac agattgggtt cgaaaaagtc tacagcctcg ccggcgggat
     6061 cgaccgctgg caggcgcaag gtttcgcgct ggtcacggaa taggcatatc tcgcgggacg
     6121 accgcgtcta ccgccctgtc tttacatctg tttttaccgc ttcctgcctt gcgtggcatg
     6181 gaatatgcaa aaccaccgca tcacaacaag atcgtcgggc agctattctt cccaataatc
     6241 cgcatggaac cgcaatcctc caccaaggcc gtttcgacca acgtcgtttc aacgaccggt
     6301 actggcatgg ttgcgggagt gcgccagatg ttcagggcga ccaggatgct gcatgagcgc
     6361 gagaagtcac gcatccgtga agagatcgcg cagattcccg gactgatggc gctgctgatg
     6421 aagccgcgca atggattgaa atggacccga acggaacgcg cggaactgcg gacacaactg
     6481 cgtcgccttt cgcggctgag catttatctc gcgctggcta cgctgccctt caccagcctg
     6541 acgctgccac tggtggcgtg gtggctcgac cagcgccagc aaaaacgcac ctgatagtcg
     6601 ccctaaaatc cctaacaaaa agaaaaaaaa tcagacagga tttacacgat taaaaaacat
     6661 gattaacagg attatttctt tttaaatttt aaaatcctta atcctgtaaa tcctgaaaaa
     6721 tcctgttaat cctgtctatt ctatttttat tgaagctcca agcattatca ggagcgaaaa
     6781 taaaccagat acccggccag tatcccgtaa aacaccagca tggccagcag attccagtgg
     6841 ccattgttgc ggtacaaaac gctccaattg aaaaaccgca cagcgggatc ggacagccgg
     6901 ccgaacgacg gcagaaaacg attcacgcgc gcgcggtaat cgctgtacgg ctggcccaac
     6961 agctccgcga ggcgcttctc ctcacggccc acgcggttga ccatgtagaa ataataaaaa
     7021 acggtgtaag cgatgatgag gtagtaattg ccgaacagca gcatgacacc cagtatcaga
     7081 aagaagcggc cgagatacat cggattgcgc accatcacat aaggcccgcg cgcggttagc
     7141 tgctcgttct tcaccagcga ggcgaacgac cagagctgaa ttgcttcgcc aaacagcgaa
     7201 accagaaaag ccacgagcag tcgcgagaca tccgcgtagc gcgccagcag caccaacacc
     7261 gccagaaaca acacgtaacg gagtttgaga aaaaacttcc gtgcaaccgg atggttgaaa
     7321 aagccgtgga tgacgtcgag catgaactgg tctgattatt gatccggcac aacggtcgcg
     7381 attattgagg cggcacgagt ctattgcaag cgccacgacg ctatatgaag acggacacgg
     7441 tattcccgac aatgcaggga tattaccggc ggaaacatga aaacagggta ggaaagattc
     7501 gcgggaaact atacaatcct gccccgagat tttcccttgg ctattcggcc catcttacat
     7561 gcttgattat caggcacccc agtgcaatgt cttctgggtg cgcttttttc cttttctgcg
     7621 ctggtggccg cgggtcaccc gcgaaacgct caaggccgac ctgatcgccg gcttcaccgg
     7681 cgccgtcatc gttctgcccc agggcgtggc cttcgccatg atcgccggcc tgccgccggt
     7741 ctacggcctg tacacggcca tggtgccgcc catcatcgcc gggttgttcg gttcctcgcg
     7801 ccatctcatt tccggcccga ccacggcgat ctcgatcgtc gtgttcgcca ccatcacgaa
     7861 attcgccgaa ccgggcagcg ccaaattcat cagcatggcg ctcacgctga ctttcctcgc
     7921 gggcgtctac cagttcgtgc tcgggctggc gcgcatgggg gcgctggtca acttcgtatc
     7981 gcattcggtc gtggtcggct tcaccgccgg tgcggcgatc ctgatcgcca ccagccagct
     8041 caagcatttc ctcggcatca atatcccctc gggcgagtcg ttcctgcaca cctggcagga
     8101 catcttccgc cagatcggtg acatcgatcc ctacgtcacg gcgattgccg ccgtcaccct
     8161 gatcgcgggc attctgttca tgcgctttaa accgcgctgg ccgggcatgc tgttcgccat
     8221 gatcatcggc agcgggctgg cggccctgct ggcctacgaa accaagggca tcaggctcgt
     8281 cggcgagatt ccggcgcaac tgccgccgtt ctccatgccc gacctgtcgc tcaaatcgct
     8341 caaggcgctc ggcagcggcg cgctcgccat cgccatgctc ggtctggtcg aggcggtctc
     8401 gatcgccaaa tccgtcgcgc tcaagtccgg ccagcgcatc aatggtaacc aggaattcat
     8461 cggccagggc ctggccaaca tggtcggcag cttcttttcg agttacgcct cttccggctc
     8521 gttcacccgc agcggcatca attacaccgc cggcgcgatc acgcccatgg ccgccatctt
     8581 cgccgccatt tcgctggccc tgattgtgct cgccgtggcg ccgctcgcgg cgcacctgcc
     8641 gatcgcggcc atggccggta tcctgctgat cgtggcgtac cggctcatcg acttccacca
     8701 catcgccaac atcctgcgca ccagcaagcg tgaaacggcg gtgctgctga ccacgtttct
     8761 ggccacgctg ttcgtggaac tggagttcgc catctacgtc ggcgtgatgc tgtcgctggt
     8821 gatttacctg atgcgcacct cgcgccccgg catcgtcgtg cgcacgcccg atccgcactc
     8881 cgcgggacgg cacatggtca ccgatccgaa actgcccgag tgcccgcagc tcaagatcgt
     8941 gcgcgtggac ggctcgctgt tcttcggcgc cgtggatcac gtgcagggcg agtttcagcg
     9001 ctttcaggcg gagaaccctg ggcagaaaca tctcatgatc atcggcaacg gcatcaactt
     9061 cgtcgacatc gccggggccg agatgctggt gcaggaggcg cggcggcgcc gcaacctggg
     9121 cggcggactc tacctgagca aggtgaagga ggaggcctgt cgcatcctga agcgcggtgg
     9181 cttcgtcgat cacctcgggg acgagcacat ctttgtcacc aaggccgacg ccatccagac
     9241 cattttcgcg aaactcgacc gctccatctg cgagcgctgc gacaagcgga tttttctgga
     9301 atgtcagaag atcgaataca agggtgacaa gccgctgccg gcggacaaac aggcggcggc
     9361 gtgagtttga tgtaggttgg gttgaggcac gaagtgccga aacccaacaa cggcaccgat
     9421 gcgttggggt tcgcttcgct caccccaacc taccggggga ttttgagaca agttctagaa
     9481 gcggtcagcg agcaccaagg tccagaccaa cacgaccagc acgagggaaa caaacaccgc
     9541 cgccgaggcg atgtccttgg cctgtccgga cagttcgtgt ggatcgcgtc cgacacgatt
     9601 aaccaccgcc tcgatcgccg aattcagcag ctcgaccagc agcaccagca tcagactgcc
     9661 gaccagcagt gagcgctcca ccccgtcgcg cccgagccag aatccgagcg gcgccaggac
     9721 aacaaacagc gccagctctt ggcgaaacgc cgcctcgttt ttcagcgccg ccctgaaacc
     9781 ggcggcggaa taacccgccg cgcgccatac ccgacccaga tcgaacaggc ctttccatcc
     9841 tttggtccgt aatcggcgca tccctgcgcc acttctcagt ggcgacgcca tgtcagccgg
     9901 cctgcttcca ggtcaggtcg agttcgcgcg cggcgcgcac gtcatccagg cgtttcaccg
     9961 gcagggtatg cggcgcgccc ttgacgacgt cggggctgga acgcacctcg cgcatgatct
    10021 cttccatcgc ggcgatgaaa cgatcgagtt cttccctggc ctcggtttca gtcggctcga
    10081 tcaggtgaca ctccggcacc agcagcggga aataggtggt cggcgcgtgg aagcccttgt
    10141 cgagcaggcg cttggcgaca tccatggcgc tcacgccggt ttcgtccttg agcttcttga
    10201 aggtaagcac gaactcgtgc gtcgcgcgcc gcttcggaaa cgccatgtcg aacccacgtt
    10261 tcgcgagttc cgccatgatg tagttggcat tgagcgtcga gaactcggcc acccgatgca
    10321 tgccctcgcg tcccagcagc cgtgcgtaca cataggcgcg caacagcacg ccggcgttac
    10381 ccatgttggc cgacagtctt ccgatcgtct gtggccgggt cttttcagtt tcccacagat
    10441 acttgtcctg caccttgccc acgatcggca ccggcagaaa cggctcgagc cggctgctcg
    10501 ccgccaccgg tccggcgccc ggtccgccgc caccatgc
//
LOCUS       DBNGZE010000149         6355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1202365, whole genome shotgun sequence.
ACCESSION   DBNGZE010000149 DBNGZE010000000
VERSION     DBNGZE010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1202365"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC16FV_03815"
     CDS             <1..530
                     /locus_tag="AC16FV_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR2849224.1"
                     /translation="GGIFTGSDAVEFLEAGAAAVQVATRFTVTKECGLPDKVKQEYFK
                     AKEEDIEVNLISPTGYPMRMLKNSPAIGAGIRPNCEAYGYLLDGSGNCAYIEAYNREL
                     EKHPDEENISVMDKTCLCTHMRNFDCWTCGHYTYRLKDTSRKLPDGSYQLLNAEDVFK
                     DYLYSKDHLIALPAA"
     gene            complement(581..1147)
                     /locus_tag="AC16FV_03820"
     CDS             complement(581..1147)
                     /locus_tag="AC16FV_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HIR2849225.1"
                     /translation="MKRTEHFLEVLMFNSRWLLAPFYLGLVVSIALLLIKFGQEFFHM
                     LPTIFAMSESEVILAILTLVDMSLVANLLIIIIFSGYENFVSKIDTADHEDRPDWMGK
                     VDFSGLKVKLIASIVAISAIELLKAFVNVTNIGVASDIGVWTVADKQLAWKVGIHFVL
                     IVSGVLFAVMDRVAEGTKTHHTGKKHDG"
     gene            complement(1224..1619)
                     /gene="msrB"
                     /locus_tag="AC16FV_03825"
     CDS             complement(1224..1619)
                     /gene="msrB"
                     /locus_tag="AC16FV_03825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR2849226.1"
                     /translation="MKYKVWKNDEEWKKILTPEQYKVTRQKGTERAFTGAYHDCHDQG
                     VYKCVCCGAELFSSDTKFDSGTGWPSFHSPMKSENVAEEDDSSFFMRRIEVHCSQCGA
                     HLGHVFPDGPQPTGLRYCINSASLKLDKK"
     gene            complement(1637..2311)
                     /locus_tag="AC16FV_03830"
     CDS             complement(1637..2311)
                     /locus_tag="AC16FV_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HIR2849227.1"
                     /translation="MMLRIPSVLDPNQLDTVRKLLADAKFVDGKLSAGAAAQRVKHNQ
                     ELDKGARQMDILNNIVMNGLVQHPVYRAGALPLRVAAPYYARYTPGMAYGDHLDDPIM
                     GADGVLYRSDIAITIFLNGPDQYDGGELVIRTAFGENEVKLPAGDAVMYPSSSLHHVN
                     KVTRGERLVAVTWVQSLVRDPARRELLYELHQAREKLLKISPEAEETAQVNTAYMNLI
                     RMWGDI"
     gene            complement(2353..3066)
                     /locus_tag="AC16FV_03835"
     CDS             complement(2353..3066)
                     /locus_tag="AC16FV_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR2849228.1"
                     /translation="MSALIILTLVCALTYTFEIVFGLTGTILMLPLLSFLYDAKTLVI
                     YSVLPQILVAVIGLVRSLKKVDRQFMAGMLFFAGLGAIVGFALFYYFSASVFQVLLAS
                     AITAAGLFLVIAPHRAKFNPAGMRVMDTLAGTSQALFGISGPIAMTRLLATYRDKNTV
                     RHYAFAFFLAMNGLRAIEYTIHGTYTEETLWMMAVSAPFLAVTLWFSNQLHLHINERM
                     FRKVVSWMILVGGLSLFYR"
     gene            complement(3124..3696)
                     /locus_tag="AC16FV_03840"
     CDS             complement(3124..3696)
                     /locus_tag="AC16FV_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR2849229.1"
                     /translation="MHEVKPLSFIYEIKNALPGELCRDMIRRFEANTDQQYAGRIGQN
                     QSEESSVKKTTDLRISGRADWKDVDRALMQSLAKNFNEIGQAFPFFAANSFKDIGYNM
                     QRYRPGEYYHWHVDGGPGEFSQRQLVALWYLNNVEGPGGETEFPLQEISVKPEEGKLV
                     LFPPFWTHVHRAVTMEKGVKYIATTWICFT"
     gene            3781..4944
                     /locus_tag="AC16FV_03845"
     CDS             3781..4944
                     /locus_tag="AC16FV_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HIR2849230.1"
                     /translation="MSLKQIIASLFRASAPVGHKEKIVSAISAFVAILLTGILSSSLG
                     HSALPLMIASMGASSVLLFAAPHSPMAQPWSFVGGHMVSALIGITCYMLIPNPFVAAA
                     LAVALAIFAMHWLRCLHPPGGATALAMVISGQELHALGYGALLSPVGLNVITMMILAL
                     AVNNLFPGRRYPMLLPAATGEKPTPPALAFGRMTLNKEDIESALKDMNAYIDVAEKDL
                     EEIYSRASLHHMRRHMGEVTCRDIMARDVATAEYGDELATVWETMRQRKLKGVPVVDR
                     ARRVIGIVTIVDFLKRVDKTQHPHPLFLDRLRAFIRRTTGLTTDKPEVVGQIMSAPAM
                     TAREDTHIVSLIPLFSEHNIHHVPIVDKDGRVTGMVTQTDLSVALYRYWAAMP"
     gene            complement(5203..5778)
                     /locus_tag="AC16FV_03850"
     CDS             complement(5203..5778)
                     /locus_tag="AC16FV_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849231.1"
                     /translation="MKLNVIVDERALAVDVPPHMLHEAEDFFRKMDRDMDHGWQMGPE
                     FIENPDKVQRCQIAANKLLTSLSAANETMITLMAGYILKRLPGVTGVRIDTGGEMLHT
                     EILYEKPASTAPAPMPAVTHAQKLGKLEAMEQAGKDVTKVYQVGKSYRFAVRDLKTGE
                     WLESGPMDTEQEAYEKRMQAYKRRLDELTGQ"
     gene            complement(5775..>6355)
                     /locus_tag="AC16FV_03855"
     CDS             complement(5775..>6355)
                     /locus_tag="AC16FV_03855"
                     /inference="COORDINATES: protein motif:HMM:NF044557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sfum_1244 family protein"
                     /protein_id="HIR2849232.1"
                     /translation="EKIEEWRWNRKNEAMERALDCYSFDLDVNSALERMTENETEAMI
                     LHELGEGLAGEKLGDEWHKMITAFSRSKAEIMIRAVRDILADCLSTLPALLAANNTAS
                     LHFYFANFTGMRKHLFPEAMAAYQRWVAEKDISMLRQLVDEGQHRWLDTACTLLALYK
                     DKGEDAAAAIERLLEPATETAPYCRLNTASNS"
BASE COUNT         1233 a         1826 c         1921 g         1375 t
ORIGIN      
        1 ccggcggtat tttcaccggc agcgacgcgg tggaatttct ggaagcgggc gcggcggcgg
       61 tgcaggtggc gacgcgtttc acggtgacga aggaatgtgg cctgccggac aaggtcaagc
      121 aggaatactt caaggccaaa gaggaagaca tcgaagtcaa cctgatctcg cccaccggct
      181 atcccatgcg catgctgaag aacagtccgg cgatcggcgc cggcatccgg cccaactgcg
      241 aggcctacgg ctacctgctc gacggctcag gcaattgtgc ctacatcgag gcctacaacc
      301 gcgaactgga aaaacatccg gacgaggaaa atatttcggt catggacaag acctgcctgt
      361 gcacgcacat gcgcaacttc gactgctgga cctgcgggca ctacacctac cggctcaagg
      421 acacgtcacg aaaattgccg gacggaagtt accagctcct gaatgccgaa gatgtgttca
      481 aggattatct ctacagcaag gatcatctaa ttgccttgcc ggcggcctag ccggtcagct
      541 tctccccgag agcaagggag aatgactttt tatcgccttg ctagccgtcg tgtttcttgc
      601 cggtgtgatg ggtcttggtg ccctcggcga cccggtccat gaccgcgaac agcacgccgg
      661 aaacgatcag cacgaaatgg atgccgacct tccaggccag ctgtttgtcc gccaccgtcc
      721 acacgccgat atctgatgcg acgccgatgt tggtgacgtt gacgaatgcc ttcaggagct
      781 cgatggcgga aatggcgacg atcgaggcga tgagcttgac cttgagaccc gagaaatcca
      841 ccttacccat ccaatccggc cgatcctcgt gatcggcggt gtcgatcttg gatacgaaat
      901 tctcgtagcc gctgaagatg atgatgatca gcaggttggc caccagcgac atgtccacca
      961 gagtcaggat cgcgaggatc acctcgctct cgctcatggc gaagatggtc ggcagcatgt
     1021 gaaagaactc ttggccgaat ttgatcagga gcagggcgat ggagaccacc agacccaggt
     1081 aaaacggcgc cagcagccag cggctgttga acatgagcac ttcgagaaag tgttcggttc
     1141 ttttcatgtc ggtatggtgg acagggatgc ggttcggctg gccggcatta tggcgggatt
     1201 gccggaacaa tcaagtcttt gggttatttc ttatccagct tcagcgaggc cgagttgatg
     1261 caatagcgta ggccggtcgg ttgcggcccg tcggggaaga catgcccgag gtgcgcgccg
     1321 cactggctgc aatgcacctc gatgcggcgc atgaagaaac tgctgtcatc ctcctcggcg
     1381 acgttctcgg atttcatcgg cgagtggaag ctgggccagc cggtgccaga atcgaacttg
     1441 gtgtcggagc tgaacagctc agcgccgcag cagacacact tgtaaacgcc ctggtcatgg
     1501 caatcgtgat acgcgccagt gaaggcgcgc tcagtgcctt tctggcgcgt gaccttgtat
     1561 tgttccggcg tgaggatttt tttccattcc tcgtcgttct tccagacctt gtatttcatg
     1621 gcagcgtctc cgtggtctaa atatcgcccc acatgcggat caggttcatg taggcggtgt
     1681 tgacctgtgc ggtttcctcc gcctccggtg aaatcttgag cagtttctcg cgcgcctgat
     1741 gcaattcata tagcagctcg cgccgcgccg ggtcgcgcac caggctctgc acccaggtca
     1801 cggccacgag gcgctcgccg cgcgtgacct tattcacatg atgcaggctg gacgagggat
     1861 acatcacagc atcgcccgct gggagtttta cttcgttctc gccgaaggcg gtgcggatca
     1921 ccagctcgcc gccgtcgtac tgatccggtc cgttcaggaa aatcgtgatg gcgatatcgg
     1981 aacggtacaa cacgccatcg gcgcccatga tgggatcatc cagatgatcg ccgtaggcca
     2041 tgcccggtgt gtaacgcgca tagtagggcg cggccacgcg caagggcagg gcgccggcgc
     2101 gatagactgg gtgctgcacc aagccattca tgacgatgtt gttcagtatg tccatctggc
     2161 gcgcgccctt gtccagctcc tggttgtgct tgacgcgttg ggctgccgcg ccagcggaaa
     2221 gtttcccgtc cacgaatttc gcgtcggcca acagcttgcg cacggtgtcg agctggtttg
     2281 ggtcgaggac ggacggaata cgaagcatca tggtgaattt gtcggcatgt cgagattttc
     2341 tatgatgcag gctcagcggt aaaaaagcga gagcccaccg actaggatca tccacgacac
     2401 gacttttctg aacatccgtt cattaatatg caggtgcagc tggttgctga accacaaggt
     2461 aacggcgagg aagggcgcgc tgaccgccat catccacagg gtttcttcgg tgtaggtgcc
     2521 atggatggtg tattcgatcg cgcgcaagcc gttcatggcg aggaaaaacg cgaaggcgta
     2581 atggcgcacg gtgttcttgt cgcgatatgt cgcgagcagc cgcgtcatgg caatcggccc
     2641 gctgatgccg aacagcgctt gcgacgtgcc cgccagcgtg tccatgacgc gcatgccggc
     2701 cgggttgaat ttcgcccggt gtggcgcgat caccagaaac agtcctgccg ctgtgatcgc
     2761 cgaggccagc aacacctgga acaccgacgc cgagaaataa tagaagaggg caaaccccac
     2821 gatcgcgccc aatccggcga agaaaagcat cccggccatg aattgacgat cgactttctt
     2881 cagcgagcgc accagcccga tgaccgccac cagtatctgt ggcagcactg agtaaatgac
     2941 cagcgtcttg gcgtcgtaga gaaaactcaa cagcggcagc atcaaaatcg tcccggtgag
     3001 gccgaagaca atttcaaatg tataggttag agcgcagacg agggtgagaa tgatcagggc
     3061 ggacattttt gttgtttcgc cggtggcgat gaagaacttg ttactgctgg aacgcaacct
     3121 gtatcacgta aaacaaatcc acgtcgttgc aatatatttg acgcctttct ccatcgttac
     3181 cgcgcgatgc acgtgcgtcc agaacggcgg gaacagcacc agcttgcctt cctcgggctt
     3241 gactgaaatt tcctgcaatg gaaattcggt ctcgccgcca gggccttcga cattatttaa
     3301 ataccagagc gccacgagtt ggcgctggct gaattcgccc gggccgccgt ccacgtgcca
     3361 gtggtaatac tcgcccggcc ggtagcgttg catgttgtag ccgatgtcct tgaacgagtt
     3421 ggcggcgaag aacggaaatg cctggccgat ttcgttgaag tttttcgcga gcgattgcat
     3481 cagcgcccgg tccacgtcct tccagtcggc gcgcccgctg atgcgcaggt cggtggtttt
     3541 cttgacgctg ctttcctcgg actggttctg gccgatgcgg ccggcgtact gctggtcggt
     3601 gttggcctcg aagcggcgga tcatgtcgcg gcacagctcg cccggcaacg cgttcttgat
     3661 ctcgtatata aaggagagcg gcttgacctc gtgcatggaa ttttccgcgg ggttgttgtt
     3721 caatgtcgcg caattttgat tatcttatgc gcccttcgcg caggtcaaac cggttcttga
     3781 atgtcgctta aacagatcat cgcctcgctg ttccgcgcct ccgcgccggt cggccacaag
     3841 gaaaagatcg tctcggcgat atcggccttc gttgccattc tgttgactgg catcctgagt
     3901 tcgagcctgg ggcacagcgc cttgccgctc atgatcgctt ccatgggtgc gtcgtcggtg
     3961 ctgctgttcg ccgcgccgca cagtccgatg gcgcaaccgt ggtcgtttgt cggcgggcac
     4021 atggtctcgg cgctgatcgg catcacctgt tacatgctca tccccaatcc cttcgtggcc
     4081 gccgcgctgg cagtggcgct cgccatcttc gccatgcact ggctgcgttg cctgcatcca
     4141 cccggtggcg ccacggcgct ggcgatggtg atcagcgggc aggaactgca tgcgctgggt
     4201 tatggtgcgc tactgtcgcc ggtcggactc aatgtaataa caatgatgat cctcgcgctg
     4261 gcggtcaaca atctgttccc cggccggcgc tatccgatgt tgttgccggc cgccaccggc
     4321 gaaaagccga cgccgccggc gctggccttc ggacgcatga cgctcaacaa ggaggacatc
     4381 gaaagcgcgc tcaaggacat gaacgcctac atcgacgtgg cggagaagga cctggaggag
     4441 atttacagcc gcgcctcgct gcaccacatg cgccggcaca tgggcgaggt gacctgccgc
     4501 gacatcatgg cgcgcgacgt ggccaccgcc gaatacggcg acgaactggc gactgtgtgg
     4561 gagacaatgc gtcagcgcaa gctcaagggc gtgcccgtgg tggaccgcgc gcggcgcgtg
     4621 atcggcatcg tcacgattgt tgattttctg aagcgcgtgg ataagacgca gcacccgcat
     4681 ccgcttttcc tcgaccgcct gcgggccttc atccgccgca ccaccgggct taccaccgac
     4741 aagcccgagg tggtggggca gatcatgtcg gcgccggcca tgaccgcgcg cgaggacacg
     4801 catattgtgt cgctgatccc gctgttttcc gagcacaaca tccatcatgt cccgatcgtg
     4861 gacaaggacg gtcgtgtcac gggaatggtg acgcagacgg atctgtcggt ggcgctgtac
     4921 cgctattggg cggcgatgcc atgaggaaga gaggatttat atgaatttcc aacgcagaga
     4981 cgcagagatc gcagagtttc tctttgttag aacctgtctc tgaattgatt tcaatgtagg
     5041 ttgggttgag gcacgaagtg ccgaaacccg acaacgaagc cggtatgttg aggttcgctt
     5101 cgctcaccac aacctgcatg gaagattctt agataggttc tgatatctct gatgtttttc
     5161 tctgcgtact ctgcgtctct gcggcagaaa ctattatagg ttttactgcc cggtcagttc
     5221 atcgaggcgg cgtttgtacg cctgcattct tttttcgtag gcttcctgct cggtatccat
     5281 cggcccggat tcgagccatt cgccggtttt caagtcgcgc accgcgaagc ggtagctctt
     5341 gccgacctgg tataccttgg tgacgtcctt cccggcctgt tccatggctt cgagcttgcc
     5401 gagtttctgt gcgtgcgtga cggcaggcat cggtgctggc gcggtgctcg cgggcttctc
     5461 gtaaagaatt tcggtgtgga gcatttcgcc accggtgtcg atacgcacgc cggtgacgcc
     5521 cggcagccgt ttgaggatgt aaccggccat gagcgtgatc atggtctcgt tggcggcgct
     5581 gagcgaggtg agcagcttgt tcgccgcgat ctggcagcgt tgcaccttgt ccggattctc
     5641 gatgaattcg ggccccatct gccaaccgtg gtccatgtcg cgatccattt tgcggaagaa
     5701 atcctccgcc tcgtgcagca tgtgcggcgg cacgtccacc gccagcgcgc gttcatcgac
     5761 gatgacgttg agcttcatga gttcgaggca gtattgagtc ggcaataggg cgccgtctcc
     5821 gtcgccggct ccagcaggcg ctcgatcgcc gccgcggcgt cctcgccctt atctttatat
     5881 aaggcgagca gggtgcatgc cgtgtcgagc cagcggtgtt ggccctcgtc cacgagctgg
     5941 cgcagcatgc tgatgtcctt ttccgcgacc caacgctggt aggccgccat cgcctcggga
     6001 aagagatgct tgcgcatgcc ggtgaaattg gcgaaataaa aatgcagcga ggcggtgttg
     6061 ttcgccgcca gcagggcggg cagggtggac aggcagtcgg cgaggatgtc gcgcaccgcg
     6121 cggatcatga tctcggcctt cgagcgcgag aaggcagtaa tcatcttgtg ccattcatcg
     6181 ccgagttttt caccggcgag accttcgccg agttcgtgca ggatcatcgc ttcggtctcg
     6241 ttttcggtca tgcgttcgag cgcggagttc acgtcgagat cgaaggaata gcaatcgagc
     6301 gcccgctcca tggcctcgtt cttgcggttc cagcgccatt cctcgatctt ctccc
//
LOCUS       DBNGZE010000150         4961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1202451, whole genome shotgun sequence.
ACCESSION   DBNGZE010000150 DBNGZE010000000
VERSION     DBNGZE010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1202451"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="AC16FV_03860"
     CDS             complement(<1..162)
                     /locus_tag="AC16FV_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR2849233.1"
                     /translation="MARTTPIERYRNFGIMAHIDAGKTTATERILFYTGVSHKIGEVH
                     DGAAIMDWME"
     gene            complement(196..666)
                     /gene="rpsG"
                     /locus_tag="AC16FV_03865"
     CDS             complement(196..666)
                     /gene="rpsG"
                     /locus_tag="AC16FV_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR2849234.1"
                     /translation="MPRRREVPKREVTPDPKYRSETVAKFISVVMKSGKKSTAEKIVY
                     GALNTIAERSKKDPIEMFNQALTNVRPLVEVKSRRVGGATYQVPVEVRSGRQTALAMR
                     WVVEAARTRSGKSMAARMADELMDAADKRGNAVKKREDVHRMAEANKAFSHYRW"
     gene            complement(676..1050)
                     /gene="rpsL"
                     /locus_tag="AC16FV_03870"
     CDS             complement(676..1050)
                     /gene="rpsL"
                     /locus_tag="AC16FV_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR2849235.1"
                     /translation="MPTINQLVAKGRRRLRSKSKVPALEACPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGYEVITYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVADRKQGRSKYGAKRPKS"
     gene            complement(1480..>4961)
                     /gene="rpoC"
                     /locus_tag="AC16FV_03875"
     CDS             complement(1480..>4961)
                     /gene="rpoC"
                     /locus_tag="AC16FV_03875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR2849236.1"
                     /translation="PDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLNAPDI
                     IVRNEKRMLQEAVDALLDNGRRGRAITGANKRQLKSLADMIKGKQGRFRQNLLGKRVD
                     YSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLESKGLATTIKAAKKMVEQAGP
                     EVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPQLVEGKAIQLHPLVCAPYNADFDGD
                     QMAVHIPLSVEAQLEARALMMSTNNILSPANGDPIIVPSQYIVLGLYYMTRERINATG
                     EGKAFADVAEVHRAYNSHQVDLQAKIKVRIHEVQFDDKGKRIATHKLYDTTAGRALLS
                     EILPDGLPFEIINRVLKKKTISELINACYRRVGLKESVIFADQLMYTGFAYATRAAVS
                     FGMDDMIIPEVKAEIIGQAEGEVKAIQNQYASGLLTDGERYNKVVDIWTHTSERVAKT
                     MMDQLGSEEVTDSQGKKTRQESFNSIFMMADSGARGSAAQIRQLAGMRGLMAKPDGSI
                     IETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVCQDLVVTED
                     DCGTTEGVAKSALVEGGEIVIALRDRILGRVVIGDIRDPGNEKKVLIADGSVLDENAV
                     ALLEERNIDQVLVRSPVTCRTRYGVCRKCYGRDLGRGHLVNLGEAVGVIAAQSIGEPG
                     TQLTMRTFHIGGAASRAAAVSRIEVKTTGVARLKNLKVVKHASGNYVAVSRSGELIIQ
                     DDQGRNRERYKLPFGAVLSVHDGSKVKSGAVVANWDPHTHPIITEVGGKVTFSDLIDG
                     VTVNKQTDEITGLSTYVVLDAKHRAGTKDIRPVITLVDGKGKPVNIPGTEIPAKYMLP
                     PGAIITAEDGAKVGVGDVLARIPQESLKTRDITGGLPRVAELFEARKPKDHAILAETS
                     GVISFGKDTKGKQRLIINDKEGVEHEYLIPKGRHITVFEGETVEQGDTIVEGAPVAAD
                     ILRLLGVEALTNYIVDEIQDVYRLQGVKINDKHIEVIVRQMLRKVRVLDSGDTRFLPG
                     EQAERARVVEDNETMEAQKKKPATFEPLLLGITKASLSTESFISAASFQETTRVLTEA
                     AITGKQDLLRGLKENVIVGRLIPAGTGLAYHQQRRRERGEAKQEAKELKQSLTGTLSG
                     KPAARSGEEAVGT"
BASE COUNT          817 a         1589 c         1483 g         1072 t
ORIGIN      
        1 ctccatccaa tccatgatgg cggcgccgtc gtgcacctcg ccgatcttgt gcgacacgcc
       61 ggtgtagaac aggatgcgtt ccgtggccgt agtcttgccg gcatcgatgt gcgccatgat
      121 gccgaaattc cggtaacgtt caataggtgt tgtacgagcc acgactcaaa tcctctataa
      181 agaacgatta cgcaattacc agcggtaatg cgagaaggcc ttgttggcct cggccatgcg
      241 atgcacgtct tcgcgcttct tcacggcgtt gccgcgcttg tccgcggcgt ccatcaattc
      301 atcggccatg cgcgccgcca tggatttgcc gctgcgcgta cgcgccgctt cgaccaccca
      361 gcgcatcgcc agcgccgtct gacgccccga gcgcacttcg accggcacct gatacgtggc
      421 gccgccgacg cggcggctct tgacctcgac cagcggacgc acattggtca gggcctgatt
      481 gaacatttca atcgggtcct tcttgctgcg ttccgcgatc gtgttgagcg cgccataaac
      541 gatcttttca gccgtcgact tcttgccgct cttcatcacc acgctgatga atttcgcgac
      601 ggtctcgctg cgatatttcg ggtcaggcgt gacttcacgc ttggggacct ctctgcgtct
      661 cggcataaat tcctcttagc tcttcgggcg cttggcgccg tacttggaac gaccctgctt
      721 gcgatcggcc acgccggcgg tatcgagcga tccgcgcacc gtgtggtaac gcacgccggg
      781 caggtccttg acgcgaccgc cgcggatcag caccacggag tgctcttgca ggttgtgtcc
      841 ttcgccgccg atgtaggtga tgacctcgta accgttggtg agacgcacct tcgccacctt
      901 gcggagcgcg gagttcggct tcttcggcgt cgtggtgtac acgcgcgtgc acacgccgcg
      961 acgctgcgga caggcctcga gggccggcac cttgctcttg ctccggagac gtctgcgtcc
     1021 cttggctact aattggttaa tcgtcggcat ttgtctagtt atctttcgat ccaaaaacag
     1081 aaatctccgc cgtcgcggaa atttcgattg aattaatcgc catccaaatt tgctcgccgc
     1141 gtcatgcggc tcgccctcgc aaattacggc tcgggccagc ctttggctgt ccaattttgc
     1201 tcctggcaaa attgtcgcgc ggtcgtcgca gatattgagg atcccggcgc ttaccccgct
     1261 accggagccc tgccagccaa cctttgggat cggctttttt agaggcttca acaaagacag
     1321 gccggcggaa ttccggcctg ttctgagggg cgcgattcta gggagtcacc ccttatgtgt
     1381 caagccatta ccggcctttt atggccattt tccgggactt taattaacgc acccttgtct
     1441 tttcggcccc tgaaaacctc ggccatcagg ccccgggttt tacgtcccga ccgcctcttc
     1501 gccgctgcgg gccgcgggct tgcccgaaag cgtgccagtc agggactgct tcagctcttt
     1561 ggcctcctgc ttggcctcgc cacgttcacg ccgccgctgc tgatggtagg ccaggcccgt
     1621 gcccgccggg atcagacgac cgacaatcac gttttccttg agaccgcgca gcagatcctg
     1681 cttgcccgtg atggccgctt cggtcagcac gcgtgtagtt tcctggaacg aggctgcgga
     1741 aataaacgac tcggtcgaca gcgacgcctt ggtgatgccg agcaacaacg gttcgaaggt
     1801 ggccggcttt ttcttctggg cctccatcgt ttcgttgtcc tcgaccacgc gcgcgcgttc
     1861 ggcctgctcg cccggcagga agcgcgtatc gccggaatcc agcacgcgca ccttgcgcag
     1921 catctgccgc acaatcactt cgatgtgctt gtcgttgatc ttcacgccct gcaagcgata
     1981 cacgtcctgg atttcatcca cgatgtaatt ggtgagcgcc tcgacaccca gcagacgcag
     2041 gatgtcggcc gcgaccggcg cgccttcgac gatggtgtcg ccctgctcca ccgtctcgcc
     2101 ttcgaacacc gtgatatgac gacccttcgg aatcaggtac tcgtgttcta cgccttcctt
     2161 gtcgttgatg atgagacgct gtttgccctt tgtgtccttg ccgaaggaaa tcacaccgct
     2221 ggtctcggcc aggatcgcat gatccttggg cttgcgcgcc tcgaacaatt cggccacgcg
     2281 cggcagaccg ccggtgatgt cgcgggtttt cagcgactcc tgcgggatgc gcgcgagcac
     2341 gtcacccacg ccgaccttgg cgccgtcttc cgctgtgatg attgcgcccg gcggcagcat
     2401 gtacttggcc ggaatttccg ttcctggaat attgaccggc ttgcccttgc catccaccag
     2461 cgtgattacc ggacggatgt ccttggtgcc ggcgcggtgt ttcgcgtcca gcacgacgta
     2521 ggtggaaagt cccgtgatct cgtcggtctg cttgttgacc gtaacgccat cgatgagatc
     2581 gctgaaggtg accttgcctc ctacttcggt gatgatcgga tgcgtatgcg ggtcccagtt
     2641 ggcaaccacc gcgccgctct tcaccttgga accgtcatgc accgacagca cagcgccgaa
     2701 cggcagcttg taacgctcgc ggttgcggcc ctggtcgtcc tggatgatga gctcgcccga
     2761 acgcgacacc gcgacatagt tgccgctcgc gtgctttacc accttgagat tcttgaggcg
     2821 cgcgacaccc gtggtcttga cctcaatgcg cgacaccgcc gcagcgcgcg acgcggcacc
     2881 accgatgtgg aacgtgcgca tggtgagctg ggtgcccggc tcgccgatcg actgcgcggc
     2941 gatgacgccc accgcctcgc ccagattgac caaatgaccg cggccgagat cgcgcccata
     3001 gcacttgcgg catacaccgt agcgggtacg gcaggtcacc ggcgaacgca ccagcacctg
     3061 atcgatgtta cgctcctcga gcagggcgac ggcattctcg tccagcaccg aaccgtccgc
     3121 aatcagcact ttcttttcgt tgccggggtc gcggatgtcg ccgatgacca cgcgtccgag
     3181 aatgcggtcg cgcagcgcga tcacgatttc gccgccttcc accagcgccg acttggcgac
     3241 gccttcggtc gtgccgcagt cgtcctcggt cacgaccagg tcctggcata cgtccaccag
     3301 gcggcgcgtg aggtaacccg agttcgcggt cttgagcgcg gtgtcggcca gacccttgcg
     3361 cgcgccgtgc gtcgagatga agtattgcag cacgttcagg ccttcacgga agttcgccgt
     3421 gatcggcgtc tcgatgatcg agccgtcggg cttggccatc aggccgcgca tgccggcgag
     3481 ctggcgaatc tgggccgcgc tgccacgcgc gccagaatcc gccatcatga agatcgaatt
     3541 gaacgactcc tgcctggtct tcttgccttg ggagtcggtc acttcctcgc tgccgagctg
     3601 atccatcatg gtcttggcga cgcgctccga ggtgtgggtc cagatgtcca cgaccttgtt
     3661 atagcgttcg ccgtcggtga gcagaccgga ggcgtactgg ttctgaatcg ccttcacctc
     3721 gccctcggcc tggccgatga tctcggcctt cacttccgga atgatcatgt cgtccatgcc
     3781 gaacgacacc gccgcgcgcg tggcgtaggc aaagccggtg tacatcagct ggtcggcgaa
     3841 gatcaccgac tccttcaggc ccacgcgccg gtaacaggcg ttgatgagct cggagatcgt
     3901 cttcttcttg agcacgcggt tgatgatttc aaacggcaat ccgtcgggca ggatttccga
     3961 cagcagcgcg cgtcccgcgg tcgtgtcata aagcttgtgg gttgcgatgc gcttgccctt
     4021 gtcgtcgaac tgcacttcgt ggatgcgcac cttgatcttc gcctgcaggt ccacctgatg
     4081 gctgttgtag gcgcgatgca cctcggccac gtcggcgaag gccttgcctt cgccggtcgc
     4141 gttgatgcgt tcgcgcgtca tgtaataaag accgagcacg atgtactgcg acggcacgat
     4201 gatcggatcg ccgttggccg gcgaaagaat gttgttggtg gacatcatga gcgcacgcgc
     4261 ctcgagctgc gcctccaccg acagcgggat gtgcaccgcc atctggtcgc cgtcgaagtc
     4321 ggcgttgtat ggcgcgcaaa cgagcggatg cagctggatc gccttgccct cgaccagttg
     4381 cggttcgaaa gcctggatgc ccaggcgatg cagcgtcggc gcgcggttca acagcaccgg
     4441 atgttcgcgg atgacctctt ccaggatgtc ccacacctcg ggaccggcct gctcgaccat
     4501 cttcttcgcc gccttgatgg tggtggcgag acccttggac tcgagcttgc tgaaaatgaa
     4561 cggcttgaac aattcgaggg ccattttctt tggcagaccg cactggtgca gcttcagcgt
     4621 cggaccgacc acgatcacgg aacggccgga atagtccacg cgcttgccga gcaggttctg
     4681 gcggaagcgg ccctgcttgc ccttgatcat gtcggccagc gacttcagct ggcgcttgtt
     4741 ggcgccggtg atggcgcggc cgcggcggcc gttgtcgagc agcgcgtcca ccgcctcctg
     4801 caacatgcgc ttttcgttgc gcacgatgat gtccggcgcg ttgaggtcga gcaggcgctt
     4861 cagacggttg ttgcggttga tgacgcggcg atagagatcg ttgaggtcgg aggtcgcaaa
     4921 gcgtccgccg tcgagcggca ccagcggccg cagatcgggc g
//
LOCUS       DBNGZE010000151         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_716312, whole genome shotgun sequence.
ACCESSION   DBNGZE010000151 DBNGZE010000000
VERSION     DBNGZE010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_716312"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1481
                     /gene="miaB"
                     /locus_tag="AC16FV_03880"
     CDS             132..1481
                     /gene="miaB"
                     /locus_tag="AC16FV_03880"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR2849237.1"
                     /translation="MPRKLHIKTFGCQMNEYDSARMADALAASHGLETTDDPAEADVL
                     LLNTCSIREKAQEKVFSQLGLWRELKTARPDLIIGVGGCVASQEGAAIIRRAPYVNLV
                     FGPQTLHRLPRMLDSVLTTRRPAVDISFPEIEKFDRLPEPRVDGVRAFVSIMEGCSKY
                     CTFCVVPYTRGEEFSRPFDDVIAEIVHLAEQGVREVILLGQNVNAYRGVMHGGEFADL
                     ALLIRYVAEIDGIGRIRFTTSHPQVFSERLVAAYAEVPQLAGHLHLPVQSGSDRILAL
                     MKRGHTILEYKSKIRRLREARPDISVTTDIIVGFPGETETDFAATMNLMEEMNFDTSF
                     SFLYSARPGTPAAELADDTPREVKEARLLRLQSRNIEMAAAISRRMVGTTQRILVEGP
                     ARKSPRQLCGRTENNRVVNFDCTDTALIGRFVDVEITEALTNSLRGRLLAEPRARRA"
     gene            1543..>2476
                     /locus_tag="AC16FV_03885"
     CDS             1543..>2476
                     /locus_tag="AC16FV_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR2849238.1"
                     /translation="MKPKPKYVELSLTPADNRRLASLCGSHDEHLKQIEEYLGVEIAN
                     RGNLFRVTGTPEESHAASQLLKDLYDVTDGKGELTLAGVHAALQQLALRVPDQESDRE
                     STLRTPKRIVRGRGPRQRQYIRNILTHDINFGVGPAGTGKTYLAVACAVEALESNRVE
                     RILLVRPVVEAGERLGFLPGAIEQKIDPYLRPLYDALNEMLGFEKVARLLEKSVIELA
                     PLAYMRGRTLNESFIILDEAQNTTAEQMKMFLTRIGFGTTAVITGDITQIDLRRTQLS
                     GLKQAIEILRDVEGITFTHFTPQDVVRHALVQKIV"
BASE COUNT          482 a          776 c          746 g          472 t
ORIGIN      
        1 ttattgacgg tcctccgaaa agcgggcggc gggagccgcc cgcttttttt atgtatttat
       61 ccggcgggtt ggcaatttca ctcccgcaac agtagattta gccgcctgcc ttgctgttta
      121 atgtgcctta tatgccccgc aagctgcata tcaagacctt cggttgccag atgaacgagt
      181 acgactccgc gcgcatggcg gacgcgctcg ccgccagcca tggcctggaa accacggacg
      241 accccgccga ggccgacgtg ttgctgctca atacctgctc catccgcgag aaggcgcagg
      301 aaaaggtgtt ctcccagctc ggcctgtggc gcgagctgaa gacggcgcgg cccgacctta
      361 ttatcggcgt gggcggctgt gtcgcgagtc aggaaggcgc ggcgatcatc cggcgcgcgc
      421 cctacgtgaa cctggtgttc ggtccgcaga ccttgcatcg tttaccgcga atgctggaca
      481 gcgtgctgac cacgcggcgg ccggccgtgg atatttcctt tcccgagatc gagaagttcg
      541 accgcctgcc agagccgcgc gtggatggcg tgcgtgcctt tgtttcgatc atggagggat
      601 gcagcaagta ctgcactttc tgcgtcgtgc cctacacccg cggcgaggaa ttcagtcgtc
      661 cattcgacga cgtgatcgcg gagatcgtgc atttggcgga gcagggggtg cgcgaagtga
      721 ttctgctggg gcagaacgtc aatgcctatc gcggggtgat gcacggcggc gagttcgccg
      781 atctggcgct gctgatccgc tatgtggccg agattgatgg catcggccgc atccgtttca
      841 ccacttcgca cccgcaggtg ttcagcgagc ggctcgtcgc cgcctatgcc gaggtcccgc
      901 aactcgccgg ccacctgcac ctgccggtgc aaagcggatc ggaccggatt ctggcgctca
      961 tgaagcgcgg ccacaccatc cttgaataca aatcgaagat acgtcggttg cgcgaggcgc
     1021 gcccggacat cagcgtcacc acggatatca tcgtcggttt tccgggcgag accgagaccg
     1081 atttcgccgc cacgatgaat ctcatggagg aaatgaattt cgacacctcc ttcagctttc
     1141 tctacagcgc gcgccccggc acgccggcgg cggaactggc cgacgacacg ccgcgcgagg
     1201 tcaaggaggc gcgcctgtta cgtttgcaga gtcgcaatat tgaaatggcg gccgccatca
     1261 gccggcgcat ggtagggacc acgcagcgca ttctggtcga aggtccggcg cgcaagtctc
     1321 cgcgacaact gtgcgggcgc accgagaaca accgtgttgt caactttgat tgtactgaca
     1381 ccgccctgat cggccggttt gtggacgtgg aaatcaccga ggcactgaca aattccctgc
     1441 gtggacggct gcttgccgaa ccccgcgcac gccgcgcttg atttattggc cgcttttgcc
     1501 cttaattatt gattgagaac tcaccggaat tttattgcac gtttgaaacc caaacccaag
     1561 tacgttgaac tcagcctcac tcccgccgac aaccggcgcc tcgccagcct gtgcggaagt
     1621 cacgacgaac atctcaagca gatcgaggaa tacctgggcg tggagatcgc caatcgcggc
     1681 aacctcttcc gtgtcaccgg caccccggag gaaagccacg ccgcctctca attactgaag
     1741 gatctctatg acgtgaccga tggcaagggc gaactgacgc tcgccggcgt acacgcggcc
     1801 ctgcaacaac tggcgctgcg cgtaccggac caggaaagcg atcgcgagag caccttgcgc
     1861 acgcccaagc gcatcgtgcg cgggcgcggg ccgcgtcaac gccagtacat ccgcaatatc
     1921 ctgacgcacg acatcaattt cggcgtcggc ccggccggca ccggcaagac ctatctcgct
     1981 gtcgcctgcg ccgtggaggc gctggagagc aaccgggttg aacgcatcct gctggtgcgg
     2041 ccggtggtcg aggcgggtga gcgcctcggt ttccttcccg gcgccatcga gcagaaaatc
     2101 gatccgtatc tgcgtccgct gtacgacgcg ctcaacgaaa tgctgggctt cgagaaggtc
     2161 gcgcgcctgc tggaaaaaag cgtgatcgag ctggcgccgc tggcctacat gcgtggccgt
     2221 acgctgaatg aatctttcat cattctggac gaggcgcaga acaccaccgc cgagcagatg
     2281 aaaatgtttc tcacgcgcat cggcttcggc acaaccgcgg taatcaccgg cgatatcacc
     2341 cagatcgacc tgcgtcgcac ccagttgtcc ggcctcaaac aggcgattga aatcctgcgc
     2401 gacgtggaag gcatcacctt cacgcatttc acgccgcagg atgtcgtgcg tcacgcgctg
     2461 gtgcagaaaa tcgtcg
//
LOCUS       DBNGZE010000152         1867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1480452, whole genome shotgun sequence.
ACCESSION   DBNGZE010000152 DBNGZE010000000
VERSION     DBNGZE010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1480452"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="AC16FV_03890"
     CDS             complement(<1..939)
                     /locus_tag="AC16FV_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR2849239.1"
                     /translation="MTDSRLVFQLAFIAAGAWLIYLLAPMLTPFLVSALLAYLGNPAV
                     SRLERWRWPRALAVALVFLLFALLVAALLFFLIPALQAQISSFVAKAPTYFDWLQHTA
                     LPWLQAMLGVELKLDIEAIRQALLGHWREVGDWATAFFLYVARSGLSIVGWFVSALLV
                     PVVTFYLLLDWDKLPARVLALLPPRRRATAVRLTRETDEVLGSFLRGQLLVMLSLATV
                     YSVGLTLVGLNLALPIGIVAGLLSFVPYLGFITGLLSAAVAAYVQFQDIIMLLGVAIV
                     FLVGQMLEGLWLTPRLVGNRIGLHPVAVIFAVMAGGR"
     gene            complement(929..1546)
                     /locus_tag="AC16FV_03895"
     CDS             complement(929..1546)
                     /locus_tag="AC16FV_03895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR2849240.1"
                     /translation="MPRTGGPVNFGIAGKPMVSISQLPNLITISRIGLAPVLILLLKE
                     QEYAASLAVFAIAGVSDGLDGFIAKRFHFESRLGSILDPLADKTLLVSSYVMLTLLNH
                     LPFWLMLTVAFRDLLIVGGYLVYTSMVGPVQMRPSWLSKLNTFMQIALVIVILAQQAL
                     QQVYAPLVDALIYAVLLTTVASGLHYLWVWLVMKQVEPAKEPHDG"
     gene            1749..>1867
                     /locus_tag="AC16FV_03900"
     CDS             1749..>1867
                     /locus_tag="AC16FV_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR2849241.1"
                     /translation="MTKSRVSLTYRDAGVDIDAGDALVEAIRPIAGATKRPGVL"
BASE COUNT          447 a          605 c          546 g          269 t
ORIGIN      
        1 ccgcccaccc gccatgacgg cgaagatcac cgccaccggg tgcaggccga tccgattgcc
       61 cacgagcctg ggcgtgagcc acaagccttc cagcatctga ccgacgagaa acacgatggc
      121 cacgcccaat aacatgatga tgtcctggaa ttgcacatag gccgcgacag ccgccgacaa
      181 caggccggtg atgaagccaa gataaggcac gaaactcaag agacccgcca cgatgccgat
      241 gggtagcgcc aggttcaggc ccaccagcgt gaggccgacg gagtaaaccg tggcaagcga
      301 caacatgacc agcaactggc cccgcagaaa actgcccagc acttcatcgg tttcgcgcgt
      361 cagtcgcacg gccgtcgcgc gccggcgtgg cggcaacaag gccagcacac gcgccggcaa
      421 tttgtcccag tccagcagca aataaaacgt gaccactggg accagcaacg cgctcacgaa
      481 ccaaccgacg atgctcaacc ccgagcgggc gacataaagg aaaaaagccg tcgcccagtc
      541 gccgacctcg cgccagtgtc cgagcaacgc ctggcgtatc gcctcgatgt ccagcttgag
      601 ttcgacgccc agcatggcct gcagccatgg cagcgcggtg tgctgcagcc aatcgaaata
      661 ggtcggcgcc ttggcgacaa aagaacttat ctgcgcctgc aacgcgggga tcaggaaaaa
      721 caatagcgcc gccacaagca gcgcaaacag cagaaacacc agcgccaccg ccagcgcccg
      781 cggccagcgc cagcgctcca gacgactcac cgcgggattg ccgagatacg ccagcagggc
      841 gctgaccaga aacggcgtca gcatgggcgc gagcagatat atcagccagg ccccggcggc
      901 gatgaaagcc aactgaaaaa caagacgact atccgtcatg gggttccttt gccggctcga
      961 cctgcttcat caccagccag acccagagat aatgcaaacc gcttgcgacc gtggtcagca
     1021 acaccgcgta aatcagcgca tccaccagcg gcgcataaac ctgttgcagc gcctgctgcg
     1081 ccaggatcac gatgaccaat gcgatctgca tgaaggtgtt gagtttgctc agccagctgg
     1141 ggcgcatctg caccggcccg accatcgagg tatacacgag atagccgccc acgatcagga
     1201 gatcgcgaaa ggccacggtc aacatgagcc agaacggcag gtgattcagc agcgtgagca
     1261 tcacatagct gctgaccagc agagtcttgt cggccagcgg atcgaggatc gagccgaggc
     1321 ggctctcaaa atggaagcgc ttggcgataa aaccgtcgag accatcggac acgccggcga
     1381 tggcgaatac cgcgagcgag gcagcgtatt cctgctcctt caggagcagg atcagcaccg
     1441 gcgccaggcc gatgcgggaa atcgtaatca gattaggtag ctgggagatg cttaccatcg
     1501 gtttgcccgc gatgccaaag ttgaccggac cgcctgttct cggcaccggg ccacccaggt
     1561 accgccgcgc gccattataa tgacaggacg tgcgaagcag gactacttta gttcggctgg
     1621 gcccaagcca gcccggtcca tcggcttgac gtacgcgcat acgcgtgatt acccggcaat
     1681 tgtcggaggt ttacgccaat gccggctcgc cctaccatac gcatccctga agatttccgg
     1741 agcctgacgt gaccaaatcg cgcgtttccc tcacctaccg tgatgcgggg gtggacatcg
     1801 atgccggcga tgcgctggtc gaggccatcc ggccgatcgc gggcgcgacc aaacgccccg
     1861 gagtgct
//
LOCUS       DBNGZE010000153         4037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_369329, whole genome shotgun sequence.
ACCESSION   DBNGZE010000153 DBNGZE010000000
VERSION     DBNGZE010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_369329"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..1787
                     /locus_tag="AC16FV_03905"
     CDS             390..1787
                     /locus_tag="AC16FV_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR2849242.1"
                     /translation="MKRLRTLLFGLLVLWPGFVLYGDSTGTGKIMLLNVNGAIGPATS
                     DYVHRGLEKAKDAGAMLVILRMDTPGGLDTAMRKIIQDVIASPVPVVTYVAPSGARAA
                     SAGAYILLASHIAAMAPATNVGAATPVHIGGVPDPGGSSKPPEKEKQDKKDSADKKGK
                     DKKEKTPKPGMDEKALNDAVAYIRGLAQMRGRNVEWAEKAVREADSISAEEAVKQNVA
                     DLIAADDADLLKKLDGSKLTLQGREIVLKTQGAQILAYEPDWRNRLLATIADPNVAYI
                     LMLLGIYGLFFELWNPGYVLPGVIGGICLLLALYAFQVLPISFAGLGLILLGIAFMVA
                     EAFLPSFGALGIGGVVAFVVGSIILMDTDVEGYAVAWPLIAGVAAVSAAFFIGVVIMA
                     LKARQRSVVSGQEEMIGALGEALENFKGAGRVRVHSEEWQARSGAALKRGQKVKVIAI
                     EGLILTVEPYNPEGN"
     gene            1789..2544
                     /locus_tag="AC16FV_03910"
     CDS             1789..2544
                     /locus_tag="AC16FV_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR2849243.1"
                     /translation="MGFISWSFIPVVIVVLVFSAFRILREYERGVVFMLGRFWKVKGP
                     GLIVIIPGVQAMVKVDLRIIVMDVPPQDVISRDNVSVKVNAVVFFRVIDPQRAIIQVE
                     DYYSATNQLAQTTLRSVLGKHELDEMLSERDRLNADIQKILDAATDAWGIKVTTVEIK
                     HVDIDPTMVRAIARQAEAERERRAKVIHAEGELQAAEKLQQAAEVLSKTPQAIQLRYL
                     ETLTVIAADKNSTIVFPLPMDMIPPFLDFVKKK"
     gene            complement(2557..3087)
                     /locus_tag="AC16FV_03915"
     CDS             complement(2557..3087)
                     /locus_tag="AC16FV_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849244.1"
                     /translation="MIHRVAPALTIILLAFAPNLYAANTVLMLLEHNEDGKAVQTKVE
                     VKTGVVESKDKGKPREKWIVRAGDAISSANRPGDRAVNFYKITGGESTLLFIVKTRYF
                     QREDGKWAPQFQLNEEPLVMRGPDGRWKPLTVIQGVPSLIVQSGSALPNAEGYAASLE
                     LGFTTGTMPIDAWLVQ"
     gene            complement(3140..3541)
                     /locus_tag="AC16FV_03920"
     CDS             complement(3140..3541)
                     /locus_tag="AC16FV_03920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR2849245.1"
                     /translation="MTDPVQLPSRHTTLQLVPMVKDTNAAGDVFGGWVMSQVDIAGSI
                     AAVRRAKGRVVTVAVNAFHFVAPVFVNDLVSFYAKVVKVGHTSITVDVEVYAERGLRS
                     ADPGEIVKVTEAVLTYVAVDADRKKRPVPPE"
     gene            complement(3538..>4037)
                     /gene="hemF"
                     /locus_tag="AC16FV_03925"
     CDS             complement(3538..>4037)
                     /gene="hemF"
                     /locus_tag="AC16FV_03925"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen-III oxidase"
                     /protein_id="HIR2849246.1"
                     /translation="AVHWHRVARDACAPFGSDVYARYKKWCDEYFYLKHRNEPRGIGG
                     LFFDDFNDRNFDHAFGFMRSVGDHYLPAYLPIVQKRKHMPYGERERDFQLYRRGRYVE
                     FNLVYDRGTLFGLQSGGRTESILMSLPPLVKWRYNWSPPPGSPEAKLYDVFLKPRDWL
                     ATEAK"
BASE COUNT          780 a         1200 c         1282 g          775 t
ORIGIN      
        1 aaggtcactg ccgctggtaa cgaccgccag atcatcacca ccaaaggcga tttcaccggt
       61 aagcaccaca cccgccgcct gcgcattaag agggctgaac gccagagtag tgaagaaagc
      121 cgcgagcagc cggttttttc cacgagcttg aaacattttt tgttgatgca cggggaatat
      181 cctccattta gtggttttat tgaaaaggtg tgtcctttcg ttgcggacag gaacaacatc
      241 cggaccggta agggccaggt gacttgtctg tccgggacaa gcataaggac gtcggacctg
      301 ttttttcaag cctctggcgc cagtttgcaa catacgccgg ctggcggcga tttctcccac
      361 gatgcgcttc tctggtatgg tgtagcgtca tgaagcgctt gcgcacgctg ttgttcggtt
      421 tgctggtgct gtggcccgga ttcgtgctgt acggcgactc cacggggacg ggcaagatca
      481 tgctgctcaa cgtgaacggt gcgatcgggc cggcgactag cgactatgtc caccgcggcc
      541 tggagaaggc aaaggatgcg ggcgccatgc tcgtgatcct gcgcatggat accccgggcg
      601 gactcgacac cgccatgcgc aagatcatcc aggacgtcat cgcctcgccg gtcccggtcg
      661 tgacctacgt agctccctcc ggcgcgcgcg ccgccagcgc cggcgcttac atcctgttgg
      721 cctcccacat cgccgccatg gcgcccgcga ctaacgtggg tgcggccact cctgtacaca
      781 tcggtggtgt gcccgacccg ggtggcagtt ccaagccgcc cgagaaggag aagcaagaca
      841 agaaggactc cgcggacaag aaaggcaagg acaagaaaga aaaaacaccc aagcccggca
      901 tggacgaaaa ggcgctcaac gacgccgtcg cctatatccg cggtctggcg cagatgcgcg
      961 ggcgcaatgt cgaatgggcc gagaaagcgg tgcgcgaggc cgattccatt tccgccgaag
     1021 aggcggtgaa gcagaacgtg gccgacctga tcgccgccga cgacgccgat cttctgaaaa
     1081 aactcgatgg cagcaaactc actctgcaag gccgcgaaat cgttcttaag acgcaaggcg
     1141 cacaaatcct ggcctacgaa ccggactggc gcaaccgcct gctcgcgacc atcgccgacc
     1201 cgaacgtggc ctatatcctg atgctgctcg gcatttacgg gctgttcttt gaattgtgga
     1261 atcccggcta tgtgctgccg ggcgtgatcg gcggcatctg cctgttgctc gcactgtacg
     1321 cgtttcaggt cctgccgatc agctttgctg gcctgggcct gatcctgctc ggtatcgcct
     1381 tcatggtggc cgaggcgttc ctgccgagct tcggtgcgct cggcatcggt ggcgtggtgg
     1441 cgttcgtggt cggctcgatc attctcatgg acaccgacgt ggagggctat gccgttgcct
     1501 ggccgctgat cgcgggggtg gcggcggtga gtgcggcatt tttcattggc gtggtaatca
     1561 tggcactcaa ggcgcgccaa cgaagcgtgg tgagcggaca ggaagaaatg atcggcgcgc
     1621 tcggcgaggc gctggagaac ttcaagggtg caggacgcgt gcgcgtgcac agcgaggaat
     1681 ggcaggcgcg ttcaggcgcg gcgctcaagc gcggacagaa agtgaaggtc atcgcaatcg
     1741 aggggctgat tctgacggtg gagccgtaca acccggaggg taattaatat gggattcatc
     1801 tcgtggagtt ttattcctgt cgttatcgtc gtgctggtgt tctcggcatt ccgcatcctg
     1861 cgcgaatacg agcgcggcgt ggtgttcatg ctcggacgct tctggaaggt caagggaccg
     1921 ggtctgatcg tcatcatccc gggcgtgcag gcgatggtga aggtggacct gcgcatcatc
     1981 gtgatggacg tgccgccgca ggacgtgatt tcgcgcgaca acgtctcggt caaggtcaac
     2041 gccgtggtgt tcttccgcgt cattgacccg cagcgcgcca tcatccaggt ggaggactac
     2101 tactccgcca ccaaccagct cgcccagacc accttgcgtt cggtgctcgg caagcatgaa
     2161 ctggatgaaa tgctgtccga acgcgacagg ctcaatgccg acatccagaa gattctggat
     2221 gccgccaccg acgcctgggg catcaaggtc accacggtcg agatcaagca cgtggacatc
     2281 gaccccacca tggtgcgtgc catcgcgcgc caagccgagg ccgagcgcga acggcgcgcc
     2341 aaggtgatcc atgccgaggg tgagttgcag gcggcggaga agctgcagca ggcagcggag
     2401 gtgctgtcca aaacgccgca ggcgatccag ctgcgttatc tcgaaacgct gaccgtgatc
     2461 gccgccgaca agaactccac catcgtgttc ccgctgccga tggatatgat tccgccgttt
     2521 ctggattttg tgaagaaaaa atagcggagc tgacgattac tgcaccagcc aggcgtcgat
     2581 gggcatggtg ccggtggtga agccgagttc gagcgaggcc gcgtaaccct cggcgttggg
     2641 cagggcgctg ccgctctgca cgattaggct tggcacgccc tggatcacgg tgagcggttt
     2701 ccatctgccg tccggtccgc gcatcaccag cggctcctcg ttcagctgga actggggcgc
     2761 ccacttgccg tcctcgcgct gaaagtagcg cgtcttcaca atgaacaaca gcgtgctttc
     2821 cccgcccgtg atcttgtaga agttcaccgc gcgatcgccc ggacggttag cggaggagat
     2881 agcgtcgccg gcgcggacga tccatttttc gcgcggcttg cccttgtcct tcgattccac
     2941 cacgccggtt ttcacctcga ccttggtttg caccgccttg ccgtcctcgt tgtgttcgag
     3001 cagcatgagc acggtattgg cggcatacaa gttgggcgcg aatgccagca ggataatggt
     3061 cagggcaggg gcgacgcggt ggatcatggg aaaaagatag tggctggcct gtcgactgtc
     3121 cacccacatt cacgaagtgt cattccggcg gcacgggacg cttcttgcgg tcggcgtcca
     3181 ccgccacgta ggtcagtacc gcctcggtga ccttgacgat ctcgcccgga tcggccgagc
     3241 gcaggccgcg ctcggcgtaa acctcgacgt ccaccgtgat cgaggtgtgg cccaccttca
     3301 cgaccttggc gtagaagctg accagatcgt tcacgaatac cggcgcaacg aaatgaaagg
     3361 cgttgaccgc taccgtgacc acgcggccct tggcgcggcg cacggctgcg atcgagccgg
     3421 cgatgtccac ctgcgacatg acccagccgc cgaacacgtc gcccgcggca ttcgtgtcct
     3481 tgaccatggg caccagctgc agcgtggtat ggcggctggg gagttgcacg ggatcggtca
     3541 tttggcctcc gtcgcgagcc agtcgcgggg tttcaggaag acatcgtaaa gtttcgcctc
     3601 cgggctgccc ggcggcgggg accagttata acgccatttc accagcggcg gcagcgacat
     3661 gaggatggac tcggtgcgcc cgccggactg caagccgaac agcgtaccgc gatcgtaaac
     3721 cagattgaac tcgacgtaac gcccgcgccg gtacagctgg aaatcgcgct cgcgttcgcc
     3781 ataaggcatg tgcttgcgtt tctgcacgat gggcaggtag gccggcaggt agtgatcgcc
     3841 cacgctgcgc atgaaaccga aggcgtggtc gaaattcctg tcgttgaagt cgtcgaagaa
     3901 cagcccgccg atgccgcgcg gctcgttgcg gtgttttaga tagaaatact catcgcacca
     3961 tttcttgtaa cgtgcgtaga catcggagcc gaagggtgcg caggcatcac gcgccacgcg
     4021 atgccagtgc acggcgt
//
LOCUS       DBNGZE010000154        13201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1203490, whole genome shotgun sequence.
ACCESSION   DBNGZE010000154 DBNGZE010000000
VERSION     DBNGZE010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1203490"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="AC16FV_03930"
     CDS             complement(<1..1441)
                     /locus_tag="AC16FV_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR2849247.1"
                     /translation="MTEPLLIAKSREPIYLLPKMTNRHGLIAGATGTGKTVTLQVMAE
                     RFSAIGVPVFMADVKGDLAGVSQKGGGNPKVEERVKQLKPEGFAYQGFPVTFWDVFGE
                     QGHPVRATVSEMGPLLLARLLNLNETQEGVLNLIFKIADDNGWLLLDLKDLRAMAQHV
                     GDNAKDFTTAYGNISGASIGAIQRALLTLENQGAENFFGEPALNLDDLMQTQDGRGMI
                     NILAADKLMQAPKVYATFLLWLLAELFERLPEVGDRDKPKLVFFFDEAHLLFSDAPPA
                     LLEKIEQVVRLIRSKGVGVYFVSQNPLDIPYVVLGQLGNRVQHALRAFTPRDQKAVRA
                     AAETFRANPKLMIETVITELGVGEALVSMLDEKGSPGIVERAFICPPQGRLGPISSAE
                     RKALIDASAIMGQYEKAVDRESAYEILKKRTAQTAAPGATAPEKSTAGTVIAMARITR
                     DFIFSPGVGKRPEKYHGTALSAYLCSPYSG"
     gene            complement(1451..2632)
                     /locus_tag="AC16FV_03935"
     CDS             complement(1451..2632)
                     /locus_tag="AC16FV_03935"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR2849248.1"
                     /translation="MSYTSFNPPVRTLMGPGPSDVHPRVLTAMARPTIGHLDPAFVAM
                     MEEIKALLQFAFQTNNALTIPISAPGSAGMESCFVNLVEPGEKVVVCINGVFGARMKE
                     NIERAGGVPIVVEDAMGTPVNPNKLEDALKAHRDVRIVAFVHAETSTGVQSDAKSIAA
                     IAHHRDCLTIVDTVTSLGGSPLKVDEWDLDAVFSGSQKCLSCTPGLSPLTLNDRAVDK
                     IKQRKTRVQSWFFDLNLLMGYWGSGKRAYHHTAPINTLYGLHEALAMLQEEGLEHSWA
                     RHQHHHLALRAGIEAMGLSFLVKESVRLPQLNAIVVPEGVDEAVLRSRLLNDYNLEIG
                     AGLGTLAGKIMRIGLMGYGANMKNVMYCLEALEAVLADMRAPIRTGVAMNAAQSVAVL
                     N"
     gene            complement(2741..3505)
                     /locus_tag="AC16FV_03940"
     CDS             complement(2741..3505)
                     /locus_tag="AC16FV_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR2849249.1"
                     /translation="MAKTAASSIQVIDRVTALLEAITTHAEPASLKFLSAETRLHPST
                     AFRILNALASHGLVERTPAGRYALGVKLLHFGSHVHGKVDLLREAKPIMESLCAQVGE
                     TVNLTVREGDEVVYVERVISNRMMRVEQVIGSRAPLHVTAVGKLFLSEGGEKACREYA
                     RRTGLPRYTPNTLTQITRLWNQIANAPKQGFALDNEEAEQGVSCIGVPVRDAHGTMVA
                     GLSISAPRERRQQSWVALIKKAGTDLSARLGYAAKS"
     gene            3694..5457
                     /gene="gcl"
                     /locus_tag="AC16FV_03945"
     CDS             3694..5457
                     /gene="gcl"
                     /locus_tag="AC16FV_03945"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HIR2849250.1"
                     /translation="MAKMTATEAAIRVMESEGVELTFGVPGAAILPLYQAMNGSKIKH
                     VLVRHEEGGTHSAEGYTRAASGRIGICIDTSGPAGTNMITGLYSAMADSIPILCITGQ
                     APRAKLHKEDFQAVDIAAIAKPVTKWAITVMEGAQVPRAFRYAFHLMRSSRPGPVLID
                     LPLDVQKEIIEYDPDSDAPMEVFKPRPHRKQIEKALDMMMAAQRPLIFSGGGVISADA
                     SDLLVELAELTQTPVIPTLMGWGSIPDDHPLNAGMMGLQTQHRYGNATFLRSDFVLGI
                     GNRWANRQTGSVEVFTKGRKFVHVDIDPMQIGRVFCPDLGIVGDAKYALEEFVAAARA
                     RKQAGKIVSRKQWIDEVQERKFTMQRRTDFDNVPIKPQRVFQEINNVFGDDACFVTAI
                     GLYQIASGQFQKVNKPRNYIVCGQAGPLGWEISACIGAKLANPDKEVVGVVGDYSFQF
                     LIEELAVAAQHKVPLVMVLINNAYLGLIRQAEIAYKMDYEVQLSFNNINCPEIGDYGV
                     DHVKAVEALGCLAVRVFDPKQMADAFRAARRVSTEKQVPVVVEVITERVTNISMGPEI
                     DKINEFEEIYDRKPEKKTQAA"
     gene            5534..6316
                     /gene="hyi"
                     /locus_tag="AC16FV_03950"
     CDS             5534..6316
                     /gene="hyi"
                     /locus_tag="AC16FV_03950"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HIR2849251.1"
                     /translation="MPKFAANLTMLFNEVDFLDRFAAAAKAGFRGVEYLFPYAYDKNQ
                     LADKLKTLKLTQVLHNLPAGDWAGGERGIGCLPARVGEFQDGVGKAIEYAAALGCKQI
                     NCLAGIAPKDATPDALRKTFVENLRFAAGKLKTAGIKLLIEPINTFDIPGFYLFRTAQ
                     ALEIMNDVGSDNLFLQYDVYHMQRMEGELAASLKKNLDRIRHIQIADNPGRNEPGTGE
                     INYSFLFRHIDQIGYDGWIGCEYKPATNTVTGLGWAAAYLSH"
     gene            6482..7360
                     /gene="glxR"
                     /locus_tag="AC16FV_03955"
     CDS             6482..7360
                     /gene="glxR"
                     /locus_tag="AC16FV_03955"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HIR2849252.1"
                     /translation="MKIGFIGLGIMGRPMAQNLMKGGHTLYLFSRSGVPQDLIGDKGI
                     ACQSPKEVAKNSEIVITMVPDTPHVESVLFGKDGVAEGLSAGKIVVDMSSISPIETKK
                     FAAQINKLGCEYIDAPVSGGEVGAKNAALTIMCGGSQATFDKVKPLFELMGKNITLVG
                     GNGDGQTCKVANQIIVALTIEAVGEALLFASKAGADPAKVRQALMGGFASSKILEVHG
                     ERMIKRTFNPGFRIELHQKDLNLALSGARALQLSLPNTATCQELFNACAANGGSAWDH
                     SAMVRALEMLAKHEIH"
     gene            7526..8761
                     /gene="glcF"
                     /locus_tag="AC16FV_03960"
     CDS             7526..8761
                     /gene="glcF"
                     /locus_tag="AC16FV_03960"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR2849253.1"
                     /translation="MQTALADFIRHTPQGIEADAVLRTCVHCGMCNATCPTYQLLGDE
                     LDGPRGRIYLIKQMLEGAAVTRSTLTHLDRCLTCRNCETTCPSGVQYHKLLDIGRAIA
                     EQKVERGRFERLKRWMLRAVIPYPARFTPLLRLGQLFRPLLPSFLKRSVPPRQSATPW
                     PTTEHARTMLVLEGCAQPAIAPNINAAAARVLDRLGVRLIAASGAGCCGAASHHTSGM
                     TQGLDFARRNIDAWWPHVEAGAEAIVMTASGCGVHVKEYGQLLKDDPAYAARAGKISE
                     LTKDIGEVLSREDLSKLSSGAKPAMKVAFHSPCSLQHGQKLNGVVEKILREKGVTLVA
                     VPDAHLCCGSAGTYSILQKELSQRLLRNKVGALESGQPDVIATANIGCLAHIQSGTTL
                     PVRHWIEILDAPPSQSMGL"
     gene            8899..9090
                     /locus_tag="AC16FV_03965"
     CDS             8899..9090
                     /locus_tag="AC16FV_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849254.1"
                     /translation="MKLLLDNLFLLGQVMSLIGLAWGAWLVLRDSLFDTLCPGHKASG
                     SVMSLAACVLQPFRRVARA"
     gene            9382..10362
                     /locus_tag="AC16FV_03970"
     CDS             9382..10362
                     /locus_tag="AC16FV_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR2849255.1"
                     /translation="MLVAAAVMLGMLAPASAAGWEPTKPVEFVIPAGTGGGADQMARL
                     IQGVVTKHNLMKQPMVVVNKSGGAGGEGFLYLKESKGDPHKIIITLSNLFTTPLATGI
                     PFNWKDLTPVAMLALDEFVLWVNADSPYKTAKEYLDAVKAGNNDQFKMAGTGSKQEDQ
                     IITVALDKFTGKKFTYVPFKGGGDVAVQLVGGHVNSTVNNPIEAVAHWRAGKLRPLCV
                     FDDQRMPYKEKVTDTMSWNDIHTCMEAGVKVDYVMLRGIFMPGGVTQDQVNYYVNLLK
                     KVRETPEWKEFMEKGAFNQSFMTGKEYANWVQKAEDTHKTLMKDAGFLAQ"
     gene            10473..10994
                     /locus_tag="AC16FV_03975"
     CDS             10473..10994
                     /locus_tag="AC16FV_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR2849256.1"
                     /translation="MSDNGHHEDGAHHRGVSTKAVEIVVALLTLVLGLVVIFDSRRVG
                     AGWADDGPQAGYFPFYIGLILCLASIWTLWRVLFSGKDVAAGVFVSHKKLRLVLSVLI
                     PSLIYVAAIYFIGIYVASAVFIGAFMYWHGRFAWSRIIPVSLSVPIAMFLLFEIWFLV
                     PLPKGPLEALIGY"
     gene            11084..12610
                     /locus_tag="AC16FV_03980"
     CDS             11084..12610
                     /locus_tag="AC16FV_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR2849257.1"
                     /translation="MEEFNSLMQGFSVALTYHNLMFMFIGILLGVLIGVLPGLGGANG
                     VAILLPLTFTMPPTSAIIMLSCIYWGALFGGAITSILFNIPGEPWSVATTFDGHPMAK
                     TGRAGEALTTAFTSSFIGALIAVIVITFLAPLVAKFSLKFGPPEFFAVQLLTFCSFVG
                     LGKGHPAKTIAAMMLGFALAAVGLDTVTGTLRMTFGTSELLRGFDFLIAVIGLFGIGE
                     ILLTMEEGLAFKGVHARINPRVVWHTWKQLPKYWMTALRGTLIGCWMGITPGGATPAS
                     FMSYGIAKRFSKDGEKFGTGQMEGVMAPETAAHAAGTSALLPMLTLGIPGSPTAAVLL
                     GGLLIWGLQPGPLLFVEQKEFVWGLIASMYLGNIVGLIIVLTTVPWWAAILRVPFSII
                     APIIVVICAIGAYTVHNAMLDVALMLVFGVVGYLFKKLDYPLAPLVLALVLGDMAESS
                     FRQSMLLSRGEVSIFWSNGLVGSICGLAMLMLFWPVITKLLARLRRGNNDLDEARPVE
                     "
     gene            12749..>13201
                     /locus_tag="AC16FV_03985"
     CDS             12749..>13201
                     /locus_tag="AC16FV_03985"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR2849258.1"
                     /translation="MPVIAMNQEMGSQGKLVAEKLAEELGLEIVRHKIIDHVAEKMHM
                     RKSTLQRFLEGKAGLLERWGTDEASLALFKVEEILDLAAKGNVIIRGWGATHILRPIP
                     HVPCVRVGAPFGTRLKWVMNSLGTDDEDMVAEEIRHSDAAHRANMQHQF"
BASE COUNT         2581 a         3912 c         4038 g         2670 t
ORIGIN      
        1 ctccgctgta tggtgagcac aaataagcgg agagtgccgt accatgatac ttttccgggc
       61 gcttgcccac tccaggagaa aatatgaaat ctcgagtgat cctagccatg gcaataacag
      121 tgccggcagt acttttttct ggcgccgtgg caccgggcgc ggcggtctgg gctgtgcgtt
      181 ttttgaggat ttcgtaggct gattcacggt ccacggcctt ttcgtactgg cccatgatgg
      241 cggaggcgtc gatcagggcc ttgcgctcgg cgctgctgat cgggccaaga cgtccttggg
      301 gcgggcagat gaaagcgcgt tccacgatac ccgggctgcc tttttcatcc agcatcgaca
      361 ccagcgcctc gcccacgccg agctcggtaa tgacggtttc aatcatgagt ttggggttgg
      421 cgcggaaggt ctcggccgcg gcgcgcaccg ccttttgatc gcgcggcgtg aaggcgcgca
      481 gcgcgtgctg cacgcggttg ccgagttgcc cgagcactac gtatggaata tcgagcggat
      541 tctgcgacac gaaatacacg cccacaccct tggagcgtat caggcgtacg acctgctcga
      601 ttttttccag cagtgccggc ggcgcgtcgc tgaagagcag atgcgcctcg tcgaagaaaa
      661 acaccagttt cggtttgtcg cgatcgccga cttcgggcag tcgttcgaaa agttccgcca
      721 gcagccacag caggaaggtc gcatagacct tgggcgcctg catgagtttg tcggcggcaa
      781 ggatgttgat catgccgcgt ccgtcctgtg tctgcatcag gtcgtcgaga ttcagcgccg
      841 gttcgccgaa gaaattttcc gcgccctgat tctccagtgt gagcagcgcg cgctggatgg
      901 cgccgatcga ggcgccggag atgttgccat aggcggtggt gaagtccttg gcgttgtcgc
      961 cgacgtgctg ggccatggcg cgcagatcct tgagatcgag cagcagccag ccgttgtcgt
     1021 cggcgatctt gaaaataagg ttgagtacgc cttcctgggt ctcattcaga tttaacaggc
     1081 gtgccagcag cagcggcccc atctcggaaa ccgtggcgcg caccggatgc ccctgttcgc
     1141 cgaacacatc ccagaatgtc accgggaatc cctggtacgc gaagccttcg ggcttcagct
     1201 gcttgacgcg ttcttccacc ttgggattac cgccgccttt ctggctaaca ccggccagat
     1261 cgcctttgac atcggccatg aacaccggca cgccgatggc gctgaagcgc tcggccatca
     1321 cttgcagcgt gacggtcttg ccggtgccgg tggcgcccgc gatcaggcca tggcggttgg
     1381 tcatcttggg caggagataa atcggttccc tgcttttggc gatcagcagc ggttcggtca
     1441 tggcgatttt tcagttcagg acggcgacgc tctgcgcggc gttcatggcg acgccagtgc
     1501 gaatcggggc acgcatgtcg gccagcacgg cctcgagcgc ctccaggcag tacatgacat
     1561 ttttcatgtt ggcgccgtag cccatgagtc cgatacgcat gatcttgccc gcgagcgtgc
     1621 cgagtccggc gccgatttcg aggttgtagt cgttcaacag gcggctgcgc aggacggcct
     1681 cgtccacgcc ctccggcacc acgatggcgt tcagttgtgg cagacgcacg gattccttga
     1741 cgaggaaaga aaggcccatg gcctcgatgc ccgcgcgcaa cgccagatga tgatgctggt
     1801 gccgcgccca ggaatgttcc agaccttctt cctgcagcat cgccagcgcc tcgtgcagtc
     1861 catataaggt attgatcggc gccgtgtggt ggtaggcgcg cttgcccgag ccccagtacc
     1921 ccatcaacag attgagatcg aagaaccagc tttgcacccg ggttttgcgc tgcttgatct
     1981 tgtccaccgc gcggtcgttc agcgtgaggg gcgacaaccc cggggtgcag gacaggcatt
     2041 tctgcgagcc ggagaagacg gcatcgagat cccactcatc caccttcagg ggcgaaccgc
     2101 cgagcgaggt cacggtgtcc acgatggtca ggcaatcgcg gtggtgcgca atcgcggcga
     2161 tactcttggc atccgattgt acgccggtgg aggtttcggc gtgtacgaag gcgacgatcc
     2221 ggacatcacg atgggccttg agcgcatcct cgagcttgtt tggattcacc ggtgtgccca
     2281 tggcgtcctc gaccacgatc ggcactccgc ccgcacgctc gatgttttcc ttcatgcgcg
     2341 cgccgaacac gccgttgata cacacgacta ctttttcacc cggctccacc aggttgacga
     2401 agcaggactc catgccggcc gaaccgggcg cggaaatggg aatggtcagg gcattgttgg
     2461 tctggaaggc gaactgcagc agcgccttga tttcctccat catggcaacg aaggcggggt
     2521 cgaggtggcc gatggtcggg cgcgccatcg ccgtgagcac gcgcggatgt acatccgagg
     2581 gtcccggtcc catcagggtg cgaaccgggg gattgaagga tgtgtaactc atgctttatc
     2641 tcattcttgg ggctgcggga agggcgcagt ataaggtcat taaaaagaat ggcgcaaacc
     2701 ggatgctcat gcggcgccgg ttagggggaa tacaggaaaa tcaggacttg gcggcgtagc
     2761 cgagccgggc ggacaggtca gttccggctt tcttgatcag agccacccag gactgctgac
     2821 gtcgttcgcg cggcgccgag atggacaggc cggcgaccat ggtgccatgg gcgtcgcgta
     2881 ccggcacgcc gatgcagctc acgccctgtt ccgcctcttc gttgtcgagc gcgaagccct
     2941 gcttgggcgc gttcgcgatc tggttccaga gccgggtgat ctgagtaaga gtgttgggcg
     3001 tgtaacgcgg caggccggtg cgccgcgcgt attcgcggca ggccttctcg ccgccctcgg
     3061 agagaaacag tttgcccacc gcggtcacgt gcagtggcgc gcgactgccg atcacctgtt
     3121 ccacgcgcat catgcgattc gagatgacgc gttcgacgta aaccacctcg tcgccttcgc
     3181 gcacggtgag gttcacggtc tcgcccacct gcgcgcacag cgactccatg atcggcttgg
     3241 cctcgcgcag cagatccacc ttgccatgca catggctgcc gaagtgcaac agcttcacgc
     3301 ccagcgcgta gcgcccggcc ggcgtgcgtt ccaccagccc gtgactcgcc agcgcgttca
     3361 ggatgcgaaa ggcggtggag ggatgcagcc gggtttcggc cgacaggaat ttcaggctgg
     3421 ccggctccgc atgcgtggtg atggcttcga gcagggcggt gacacggtcg atgacctgga
     3481 tggaagaagc ggcggtttta gccatatttc atataatgaa atcgattatg tattgtgaaa
     3541 tatgctaacc tgcgttagcc ttttatgcaa ggtgcctgcc gtgaccggca cggaatcgtt
     3601 ttccgtgccg gatttctcgg ttgggcgata cgcaagacac gagaagcagg ctactctacg
     3661 gttccgcgtt gtgacatatc gaggaaacaa gccatggcaa aaatgaccgc aaccgaagcg
     3721 gcgatccgcg tgatggaaag cgaaggtgtc gagctgacgt ttggcgttcc gggcgccgcg
     3781 atcctgccac tgtatcaggc aatgaacggg agcaagatca agcatgtgct ggttcgccac
     3841 gaggaaggcg ggacccattc ggcggaaggt tacacgcgcg ccgcgagcgg tcgcatcggc
     3901 atctgcatcg acacctccgg cccggccggc accaacatga tcaccggcct gtattcggcg
     3961 atggccgaca gcattccgat cctgtgcatc accggccagg cgccgcgcgc caagctgcac
     4021 aaggaagatt tccaggcggt cgatatcgcc gccatcgcca agccggtgac caagtgggcg
     4081 atcaccgtca tggaaggcgc gcaggtgccg cgcgcgttcc gctatgcgtt tcatctgatg
     4141 cgctccagcc gtcccggccc ggtactgatc gatctgccac tcgacgtgca gaaggaaatc
     4201 atcgaatacg atcccgactc cgatgctccg atggaggtgt tcaagccgcg cccgcaccgc
     4261 aagcagatcg agaaggcgct tgacatgatg atggcggcac agcggccgct gatcttctcc
     4321 ggcggcggcg tgatcagcgc cgacgcctcc gacctgctgg tggaactggc cgaactgacc
     4381 cagacgccgg tcattcccac actcatgggt tggggcagca tccccgacga tcatccgctc
     4441 aatgccggca tgatgggcct gcagacccag catcgctacg gcaatgccac gttcctgcgc
     4501 agcgacttcg tgctcggcat cggcaaccgc tgggccaatc gccagaccgg cagcgtcgag
     4561 gtcttcacca agggccgcaa gttcgttcat gtcgacatcg atccgatgca gatcggccgc
     4621 gtgttctgcc ccgatctcgg catcgtcggc gacgccaaat acgcgctcga ggaattcgtc
     4681 gcggcggcgc gggcgcgcaa acaggccggc aagatcgtca gccgcaagca atggatcgac
     4741 gaggtccaag aacgcaaatt caccatgcag cggcgcaccg attttgacaa cgtgccgatc
     4801 aagccgcagc gcgtgtttca ggagatcaac aacgttttcg gtgacgatgc gtgtttcgtc
     4861 accgccatcg gcctgtacca gatcgcctcc ggccagttcc agaaagtcaa caagccgcgg
     4921 aactacatcg tctgcggcca ggccgggccg ctcggctggg aaatctcggc ctgtatcggc
     4981 gccaagctgg ccaatccgga caaggaagtt gtcggcgtgg tcggcgatta ttcgttccag
     5041 ttcctgatcg aggagctggc ggtcgccgcc cagcacaagg taccgttagt gatggtgctg
     5101 atcaacaatg cctatctcgg attgatccgt caggccgaaa ttgcctacaa gatggattac
     5161 gaggtgcaat tgagcttcaa caacatcaat tgccccgaga tcggcgatta cggcgtcgat
     5221 cacgtcaagg ccgtcgaggc cctgggctgc ctcgccgtgc gcgtgttcga cccgaaacag
     5281 atggcggatg cctttcgcgc ggcgcgccgc gtgagcacgg agaaacaggt gccggtcgtg
     5341 gtcgaagtca tcaccgagcg cgtgaccaat atttccatgg gcccggagat cgacaagatc
     5401 aacgagttcg aggagattta cgaccgcaag cccgagaaaa agacgcaggc tgcctgattt
     5461 ccgccgcctg tcgtaataac gtcgcggcgc tgtgtgccgc gatttgcgtt caatccttat
     5521 acaaggaacg acgatgccca agtttgccgc caacctgacc atgttgttca acgaggtcga
     5581 tttcctggac cggttcgccg ccgccgccaa ggccggtttc cgcggcgtcg aatacctgtt
     5641 tccctacgcc tacgacaaga accagctggc ggacaagctc aaaacgctca agctgaccca
     5701 ggtactgcac aacctgcccg cgggcgattg ggccggcggc gagcgcggca tcggctgctt
     5761 gcccgctcgc gtcggtgaat tccaggacgg tgtcggcaag gccatcgaat acgccgcagc
     5821 gcttggttgc aaacagatca attgcctcgc cggcatcgca cccaaggacg caacaccgga
     5881 cgcactgcgc aaaaccttcg tcgagaacct gcgcttcgcc gccggcaagc tcaagaccgc
     5941 cggcatcaag ctgctgatcg agccgatcaa caccttcgac atcccgggct tttatctatt
     6001 ccgcaccgcg caggcgctgg agatcatgaa tgacgtgggc tcggacaatc tgttcctgca
     6061 atacgacgtg taccacatgc agcgcatgga gggtgagctg gcggcaagcc tgaaaaaaaa
     6121 tctcgatcgc atccgccaca tccagatcgc cgacaatccg ggacgcaacg agcccggcac
     6181 cggcgaaatc aattattcat tcctgttcag gcatatcgac cagatcggat acgacggctg
     6241 gatcggctgc gagtacaagc cggccaccaa cacggtcacg ggcctcggtt gggccgcggc
     6301 gtatctgtcg cactagaagc tatctcataa atatcttaaa ccgcagatta acgcggattg
     6361 attatcaaga tgaacgcaga taaaaaagga atcctattta aaccatctgc gcttatctct
     6421 ttccttatct gcgttcatct gcggttgcct tgtctttttt gatttttgaa ggagaacaac
     6481 catgaaaatc ggatttatcg gtctcggcat catgggccgc cccatggccc agaacctgat
     6541 gaagggcggt cacactctct atcttttcag tcgctcgggt gttccccagg atctgatcgg
     6601 cgacaaaggc atcgcctgcc aatccccgaa agaggtggca aagaattccg agatcgtcat
     6661 caccatggtg ccggatacac cgcatgtcga gtccgtgcta ttcggcaagg atggcgtggc
     6721 cgaggggctg tccgcgggca agatcgtggt ggacatgagt tcgatttccc cgatcgagac
     6781 caagaaattc gccgcccaga tcaacaaact gggatgcgaa tatatcgacg cgccagtctc
     6841 gggcggcgag gtgggcgcca agaacgccgc gttgaccatc atgtgcggcg gcagccaggc
     6901 cacgtttgac aaggtcaagc cgttgttcga actgatgggc aagaacatca cgctggtggg
     6961 cggcaacggc gacggccaga cctgcaaggt ggcgaaccag atcatcgtgg cgctgaccat
     7021 cgaggcggtg ggcgaggcgc tgctgttcgc ttccaaggcc ggcgccgatc cagccaaggt
     7081 gcgccaggcg ctgatgggcg ggttcgcttc gtcaaaaatc ctcgaagtgc acggcgaacg
     7141 catgatcaag cgcaccttca atcccggctt ccgcatcgaa ctgcaccaga aggacttgaa
     7201 cctggcattg tccggcgcac gggcgttgca gctcagcctg ccgaacacgg ccacctgcca
     7261 ggaactgttc aacgcctgcg ccgccaacgg cggcagcgcc tgggaccact cggccatggt
     7321 gcgcgcgctg gaaatgctgg cgaagcatga aattcattaa agattatttc tgaaccgcca
     7381 agacgccaag aacgccaagt ttttaaatta aaaatagctt ttcttggcga ccttggcgtc
     7441 ttggcggtta atttcttttt ttaaatagaa cttccatcat gatttaggta tcgtttggcc
     7501 atccgccaac gcttacccgt gcaccatgca aaccgccctg gccgatttca tccgccatac
     7561 gccgcagggc atcgaggccg atgccgtgct gcgcacctgc gtgcattgcg gcatgtgcaa
     7621 cgccacctgt cctacctatc agctgttggg tgacgaactc gatggcccgc gcggacgcat
     7681 ttatctgatc aagcagatgc tcgaaggcgc tgcggtcacg cgcagtactc tgacgcatct
     7741 cgaccgctgc ctcacctgcc gcaattgcga gaccacctgt ccctcgggtg tgcagtatca
     7801 caagttgctg gatatcggcc gtgctatcgc cgagcaaaaa gtagaacgcg gccgctttga
     7861 gcggctgaaa cgctggatgc tgcgggcggt tattccctat ccggcgcgct tcacgccgtt
     7921 gttgcggttg gggcaattgt tccgtccgtt gctgccgtcc ttcctgaagc gttccgtgcc
     7981 gccgcgccaa tcggccactc cgtggccaac gacggagcac gcgcgtacca tgctcgtgct
     8041 cgaaggttgc gcccagcccg ccatcgcgcc caacatcaac gccgctgccg cgcgcgtgct
     8101 tgaccggctc ggtgttcgtc ttatcgcggc ttccggcgcc ggctgttgcg gcgcggccag
     8161 ccatcacaca tccgggatga ctcagggcct ggatttcgcg cgtcgtaaca tcgatgcctg
     8221 gtggccgcat gtcgaagccg gcgccgaggc gattgtcatg acggcgagtg gttgcggcgt
     8281 gcacgtgaag gagtatggtc agctcctgaa ggacgatccc gcgtacgcgg ccagggccgg
     8341 aaaaatttcc gaattgacga aagacatcgg cgaggttctg tcacgcgagg atttgtcgaa
     8401 actgtcctcg ggcgcgaagc cggcaatgaa agttgccttc cattccccgt gttcgctgca
     8461 acacgggcag aaattgaacg gcgtcgtgga aaaaatcctg cgcgagaagg gcgtcacctt
     8521 ggtcgccgtg ccggacgcgc atctctgctg cggttcggcg gggacctact ccattttgca
     8581 gaaggaactt tcgcagcggc tgctcaggaa caaggtcggt gcgctggaat ccggccagcc
     8641 cgacgtcata gcgacggcca acatcggttg tctggcgcat atccagagcg gaacgacatt
     8701 gccggttcgg cactggatcg agatactgga tgcccctccc agccaatcca tggggctata
     8761 gaagccaatt aaatcagtcg gtttttacct gtttcgcagc cggggccttg tgattccggt
     8821 atccgggccg catataaccc atataaatga aggccggtgt gtccggcatt ttctgcaaat
     8881 caatctaagg aacggaccat gaaactgctg ttagacaacc tctttctcct cggccaagtc
     8941 atgagcctga tcgggctcgc ctggggggcg tggttggtcc tgcgtgattc cctgttcgat
     9001 accctgtgcc ccggccataa agcttccggg tccgtcatgt cgctcgcggc ttgtgtattg
     9061 caacccttcc gccgcgtcgc gcgggcctag cccggccgca ccccgtcgtt ttcatctggt
     9121 aaaaaagcgt ttcgttgggc gctcaatgtt cttgagttgc ccgacttctc ccgttacatt
     9181 gagtgacggg actccggacc attccacaaa aattcatcga accggagttc cgccgtcagg
     9241 cgattgtttg tgaattcatg accaccgcgc gtgatcgctg tggcgccggc gactgataag
     9301 gcagagttca tcttcgcaac aaggatagga ggcataatgc aaaccaatac tgtaagcatg
     9361 ttatgcggca ccttgagcaa aatgctggtt gccgcagcgg tcatgctcgg catgctggcc
     9421 ccggcatccg cggcgggctg ggaaccgacg aagccggtgg agttcgtcat tcccgccggc
     9481 accggcggcg gcgccgacca gatggcgcgt ctgattcagg gcgtggtcac gaaacacaac
     9541 ctgatgaagc agccgatggt ggtggtcaac aagtcgggcg gcgcgggcgg cgaaggcttc
     9601 ctctacctca aggaaagcaa gggcgatccg cacaagatca tcatcaccct gagcaatctt
     9661 ttcaccaccc cgctggcgac cggcattccg ttcaactgga aagacctcac gccggtggcg
     9721 atgctggcgc tcgacgagtt cgtgctgtgg gtcaacgccg attcaccgta caagaccgcc
     9781 aaggagtacc ttgacgcggt gaaggccggc aacaatgacc agttcaagat ggctggtacc
     9841 ggctcgaagc aggaagacca gatcatcacg gtcgcgctcg acaaattcac cggcaagaaa
     9901 ttcacctacg ttcccttcaa gggcggcggc gatgtcgccg tgcagctcgt cggcggtcac
     9961 gtgaattcca cggtgaacaa cccgatcgag gccgttgccc actggcgcgc cggcaagctg
    10021 cgtccgctgt gcgtgttcga cgatcagcgc atgccgtaca aggaaaaggt gaccgacacc
    10081 atgtcgtgga acgacattca cacctgcatg gaagccggcg tgaaagtgga ctatgtgatg
    10141 ctgcgcggca ttttcatgcc cggtggcgtc acccaggacc aggtgaatta ttacgtcaac
    10201 ctgctcaaaa aggtgcgcga aacgccggaa tggaaggaat tcatggagaa gggcgcgttc
    10261 aaccagagtt tcatgaccgg caaggaatac gccaactggg tgcagaaggc cgaggatacc
    10321 cataaaacgc tgatgaagga cgccgggttc ctcgctcagt aacaacctcg gctggtaaag
    10381 gtgtcgagca ggaaatggaa cagggtggag attgtcgtca ggtctccacc ctgtttttgt
    10441 ctcgacacag gatgtgacgg aggagtcgga agatgagcga taacggtcat cacgaagacg
    10501 gggcccacca tcgcggcgta tcgacgaagg cggtggagat cgtcgtggcg ctgctgaccc
    10561 ttgtcctcgg actggtggtg atctttgaca gccgccgcgt cggcgccggc tgggccgatg
    10621 atggcccgca ggccggctat ttcccattct atattggcct tattctctgc ctcgccagca
    10681 tatggacgct gtggcgggtg ctgttttccg gcaaggacgt cgccgccggc gtgtttgtca
    10741 gccataaaaa actccggctg gtgctgtcgg tgctcattcc ctccctgatt tacgtcgccg
    10801 cgatttattt tatcggcatt tatgtcgcct ccgccgtgtt tatcggcgca ttcatgtact
    10861 ggcacggccg ctttgcgtgg agccggatca ttccggtcag cctgagcgtc ccgatagcca
    10921 tgtttctgct gttcgaaatc tggtttctgg ttcccctgcc caaggggccg ctggaggcgt
    10981 tgatcggcta ttgatgccgg gaccagtact ccggcttggc atgtttcaca tcagggtggc
    11041 gggatcagcg tccgctgtcc gtaacagggg aggggaagcg agcgtggaag agttcaacag
    11101 cctgatgcag ggcttcagcg tcgcgctcac atatcacaac ctgatgttca tgttcatcgg
    11161 catcctgctg ggggtgctga tcggggtgtt gcccgggctc ggtggcgcca acggcgtggc
    11221 gatcctgttg ccgctgacgt tcacgatgcc gcccaccagc gccatcatca tgttgtcgtg
    11281 catctactgg ggtgcgctgt tcggcggtgc catcacatcg atcctgttca atattccggg
    11341 cgagccgtgg tcggtagcga ccactttcga tggccatccg atggccaaaa ccggccgcgc
    11401 cggcgaggcg ctgaccacgg ccttcacctc gtccttcatc ggagccctga tcgcggtcat
    11461 cgtgattacc ttcctcgcgc cgctggtggc gaagttctcc ctgaaattcg gcccaccgga
    11521 atttttcgcg gtgcagttgc tcaccttctg cagtttcgtc ggcctcggca agggtcatcc
    11581 ggccaagacc atcgccgcca tgatgctcgg tttcgcgctc gccgccgtcg ggctcgacac
    11641 cgtgaccggc acgctgcgca tgaccttcgg caccagcgaa ctgctgcgcg gtttcgattt
    11701 tctgatcgcg gtgatcggcc tgttcggcat tggcgagatc ctgctcacca tggaggaagg
    11761 actggcgttc aagggcgtgc atgcgcgcat caacccgcgc gtggtctggc atacctggaa
    11821 gcagttgccg aaatactgga tgacggcgct gcgcggcacg ctgatcggtt gctggatggg
    11881 catcaccccg ggcggcgcga cgccggcctc gttcatgagt tacggcatcg ccaagcgttt
    11941 ctcgaaggac ggtgagaaat tcggcaccgg tcagatggag ggcgtgatgg cgccggaaac
    12001 cgcggcgcat gccgccggca ccagcgcgtt gctgccgatg ctgacactcg gcatcccggg
    12061 ctcgcccacg gcggcggtgc tgctgggcgg cctgctgatc tggggcttgc agcccgggcc
    12121 gctgttgttc gtggagcaga aagaattcgt gtggggcctg atcgccagca tgtatctcgg
    12181 taacatcgtc ggcctgatca tcgtgctgac gacggtgccg tggtgggcgg cgatcctgcg
    12241 cgtgccgttc tccatcattg cgcccattat cgtggtgatc tgtgccatcg gcgcctatac
    12301 cgtgcacaat gcgatgctgg acgtggcgct gatgctggta ttcggcgtcg tcggctacct
    12361 gttcaagaaa ctggattatc cgctggcgcc gctggtgctg gcactggtgc tgggcgacat
    12421 ggcggaaagt tcgttccgcc agtcgatgct gctgtcgcgc ggcgaggtgt ccattttctg
    12481 gtctaacggc ctggttggca gtatctgcgg tctggcgatg ctgatgctgt tctggccggt
    12541 gatcacgaag ctgcttgcgc gcctgcgccg cggcaataac gacctggacg aagcccgtcc
    12601 ggtggaatga ttaagacaag gcatccgcag atgaacgcag atactaagaa gataaacgca
    12661 gataaaaatg aagatattgt ttctctgcgt tcatcctaat atatatctgc gttaatctgc
    12721 ggattaaagt atttaaaagg agatcttcat gccggtaatc gccatgaatc aggagatggg
    12781 ctcgcaaggg aagctcgtcg ccgaaaagct cgctgaagag ctgggcctgg agattgtgcg
    12841 ccacaagatc atcgatcacg tggccgagaa aatgcatatg cgcaaaagca ccttgcagcg
    12901 ttttctcgag ggcaaggccg gtctactaga gcgctggggc accgacgagg ccagcctggc
    12961 gctgttcaag gtcgaggaaa tcctcgatct ggcggccaag ggcaatgtca tcatccgcgg
    13021 ctggggcgcg acccatatcc tgcgtccgat tccgcacgtc ccgtgcgtgc gcgtcggcgc
    13081 gccgttcggc acgcgcctca agtgggtcat gaattcgctt ggcaccgatg acgaggacat
    13141 ggtcgccgag gagatccgcc acagcgacgc ggcgcaccgg gccaacatgc aacaccagtt
    13201 c
//
LOCUS       DBNGZE010000155         5537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1898217, whole genome shotgun sequence.
ACCESSION   DBNGZE010000155 DBNGZE010000000
VERSION     DBNGZE010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1898217"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="glcE"
                     /locus_tag="AC16FV_03990"
     CDS             complement(<1..1046)
                     /gene="glcE"
                     /locus_tag="AC16FV_03990"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR2849259.1"
                     /translation="MNHDNDISKQLAEAIKTAVKKRTPLTITGSGSKRFYTGDIKGEK
                     LDVTGHRGIVSYEPTELVITARAGTPLAEIEAALAEKGQMLAFEPPWFGETATLGGTI
                     ACGFSGPRRPYAGATRDHVLGARIINGNGEILHFGGEVMKNVAGYDVSRLMVGALGTL
                     GVLLEVSLKVLPKPAREITLGFAMPADKAITTMNAWGAQPLPLSATCHVGDTLYVRLS
                     GTELGVQAARAKLGGKVLDKGDAFWRDIREHQHGFFAGGAPLWRLSVPPATAPMNLPG
                     QWLLDWGGAQRWLRSDAPASVIQRETEKAGGHATLFRRAGQNGAAFHPLPAAVATLQQ
                     NLKLAFDPHAALNA"
     gene            complement(1175..2668)
                     /locus_tag="AC16FV_03995"
     CDS             complement(1175..2668)
                     /locus_tag="AC16FV_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR2849260.1"
                     /translation="MATVIPEKATGTSIARERFAAMLREFMPRSSVLTEEEDLRPYEC
                     DGLSAYRRLPWIVALPETVEQVGRLLRLCSGQNIPVVARGAGTGLSGGALPLEDGVLL
                     SLAKFNRILEIDPLNRTATVQPGVRNLAISDAAAPYGLYYAPDPSSQLACSIGGNVAE
                     NAGGVHCLKYGLTAHNVLGIKFMTMDGEVITLGGKALDIPGYDLLALMTGSEGMLGVM
                     VEFTVKLLPKPERAQVVLAVFDEIEKAGDAVAAIIAGGFVPAGLEMMDNLAIRAAEEY
                     AKAGYPVGAAAILLCELDGTAEEVSEGIAQIRTLLLKQGAAEVRTAKDEAERLRMWAG
                     RKAAFPAVGRLSPDYYCMDGTIPRKHLAKVLKRISDLSKEYGLAVANVFHAGDGNLHP
                     LILYNANNPGELEKTEELGGKILEFCIEVGGTVTGEHGVGVEKINQMCIQFTKEELAQ
                     FHALKAAFDPKGLLNPGKAVPTLSRCAEFGRMHVHGGKLIYPDLERF"
     gene            complement(2717..3259)
                     /locus_tag="AC16FV_04000"
     CDS             complement(2717..3259)
                     /locus_tag="AC16FV_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019556372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849261.1"
                     /translation="MKDETIENHETITEERTTGLTAIIQSVPRVLVPLFALFFLSFAL
                     LYASSAIIEFIQPILSDGDLTSGLVKGLHMGVVALAVYELAQVVHQEYDKSGKPSDAV
                     WRIRRGVTRFASIVFVALVLESLIMVIKYSQQDLAGFLYYPVAIIASASLLLMSLGVF
                     IKLSGPISLDDRQANCPENE"
     gene            3423..4343
                     /gene="gluQRS"
                     /locus_tag="AC16FV_04005"
     CDS             3423..4343
                     /gene="gluQRS"
                     /locus_tag="AC16FV_04005"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR2849262.1"
                     /translation="MKSPKPYRGRFAPSPTGPLHFGSMVAAVGSFIEARHRGGDWLVR
                     IEDLDPPREMPGAVADILRTLEAFGLHWDGEVVYQSRRHALYEEALEKLKARDELYAC
                     ACSRREIADSSVKGVDGLVYPGTCRSGIAEGRAPRAWRVKVDGQKIEFNDVLQGRTMR
                     DMAADFGDFVVRRADGFFTYQLAVVVDDAAQGITEVVRGADLIESTPRQIHLQKLLGL
                     PTPGYLHLPVALSKQREKLSKQTFAAPADVARPAPLLCQVLRFLGQEVPPELADAGIE
                     DFWRWAVTHWDVKRIPRVSGVVVETPNAQN"
     gene            4481..4762
                     /locus_tag="AC16FV_04010"
     CDS             4481..4762
                     /locus_tag="AC16FV_04010"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004843 - cysteine-type deubiquitinase
                     activity [Evidence IEA];
                     GO_process: GO:0016579 - protein deubiquitination
                     [Evidence IEA];
                     GO_process: GO:0043161 - proteasome-mediated
                     ubiquitin-dependent protein catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-terminal hydrolase 14"
                     /protein_id="HIR2849263.1"
                     /translation="MATQCTHLDAIRDVSPNTSGCEECLKLGDRWVHLRMCMTCGHVG
                     CCDSSKNKHATKHFHATQHPIMRSLEPGEDWYWCYVDNIMLGRQSKRRT"
     gene            4782..5462
                     /locus_tag="AC16FV_04015"
     CDS             4782..5462
                     /locus_tag="AC16FV_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2849264.1"
                     /translation="MNKPNALKGCPLLAGIPPEDLRRDCPTARIVAIRHRGTIYRQGE
                     PGRTVFCVLDGQVTIARVSHDGATLTTAALGAGDFFGPALSGATAAEDTARAKGAVSI
                     WRAPIDEWRRLLLHHPAVAWDFVSLLALRQRQMERRLESFAFKRTEARLAQTFRELSG
                     GFATRCNHGFGQHLRLTQQELADLVGASRPVVSTILNKLRDKGVLGYTREYVCVRRIE
                     DIEKLIDS"
BASE COUNT         1037 a         1685 c         1770 g         1045 t
ORIGIN      
        1 ttggcgttca atgccgcatg cggatcgaac gccagtttca gattctgctg tagcgtggcg
       61 accgcggccg gcagaggatg gaacgctgcg ccgttttgtc cggcgcggcg gaacagggtg
      121 gcgtgaccgc cggccttttc cgtttcccgt tgaatcacgg aagccggcgc gtcgctcctg
      181 agccagcgct gtgcgccgcc ccagtcgagg agccattgcc caggcagatt catcggcgcc
      241 gtggcgggtg ggacggacag gcgccacagc ggcgcgccgc ccgcaaaaaa gccgtgctgg
      301 tgttcgcgga tgtcgcgcca gaatgcatcg cctttatcca gcaccttgcc gcccagcttg
      361 gcgcgcgcgg cctgcacccc caactcggtc ccggagagac gcacatataa ggtatcgccc
      421 acgtggcagg tggcggacag tggcagcggc tgcgcgcccc aggcattcat ggtcgtgatg
      481 gccttgtcgg ccggcatcgc gaagccgagc gtgatttccc tcgccggttt cggcagcacc
      541 ttgagcgaga cttcgagcag cacgccgagc gtgccgagtg ccccgaccat caggcgcgag
      601 acgtcgtagc cggcgacgtt cttcatcacc tcgccgccga aatgcaggat ctcgccgttg
      661 ccgttgatga tgcgcgctcc gaggacgtgg tcgcgcgtgg ccccggcata gggccggcgt
      721 gggccggaaa agccgcaggc gatggtgccg ccgagcgtgg cggtctcgcc aaaccagggc
      781 ggctcgaagg cgagcatctg acctttttcg gccagtgccg cttcgatctc ggcgagcggc
      841 gtgcccgcgc gggcagtgat gaccagctcg gtcggttcat aggaaacgat gccgcgatga
      901 ccggtgacat ccagtttttc gcctttgatg tcgccggtgt aaaagcgctt gctgccgctg
      961 ccggtgatcg tgagaggcgt gcgcttcttt accgcggttt tgatggcttc ggccagttgt
     1021 ttcgaaatgt cgttgtcgtg gttcatttca agtaataccg tcattccgta catggaatgt
     1081 acggaatcca gtgccaggga aggcatccct gcgtctggat tcccgccaat ccatggcggg
     1141 aatgacagac gtgagtaaca ttacgaaatt catttcaaaa tctctccaga tcgggataaa
     1201 tcagcttgcc accatgcaca tgcatgcgcc cgaattcggc gcaacgcgac agcgtcggca
     1261 ctgccttgcc cgggttgagc agacccttgg gatcgaaggc cgctttcagg gcgtggaact
     1321 gcgccaactc ttccttcgtg aactggatgc acatctggtt gatcttctcc acgcccacac
     1381 catgctcgcc ggttaccgtg ccaccgacct cgatgcagaa ttccagaatc ttgccaccca
     1441 gttcctcggt cttttccaat tcgccgggat tgttggcgtt gtagaggatc aacggatgca
     1501 ggttgccgtc gccggcgtga aacacattcg ccacggccag gccgtattcc ttcgacagat
     1561 cgctgatgcg cttcagcacc ttggccagat gcttgcgcgg aatggtgccg tccatgcagt
     1621 aataatccgg cgacagccgc ccgacggcgg gaaacgccgc cttgcgcccg gcccacatgc
     1681 gcagacgctc ggcctcgtcc ttggcggtgc gcacttcggc ggcgccctgc ttcagcagca
     1741 acgtgcgaat ctgcgcgatg ccctcggaaa cttcctcggc ggtgccgtcg agctcgcaca
     1801 gcaggattgc cgccgccccg accggatagc cggccttggc gtattcctcg gcggcgcgga
     1861 tggcgaggtt gtccatcatt tcgagaccgg cgggcacgaa gccgccggcg atgatcgcgg
     1921 cgacggcatc gccggctttt tcaatttcgt cgaacaccgc gagcaccacc tgcgcgcgct
     1981 ccggcttggg caggagtttg accgtgaact cgaccatcac gccgagcatg ccttccgagc
     2041 cggtcatcaa cgccagcaga tcgtaaccgg ggatgtcgag tgccttgccg ccgagcgtga
     2101 tcacctcgcc gtccatggtc atgaacttga tgcccagcac gttgtgcgcg gtgaggccgt
     2161 atttcaggca atgcacgccg ccggcgtttt ccgcgacgtt gccgccgatg gagcaggcga
     2221 gttgcgacga gggatcgggc gcgtagtaca ggccgtaagg cgcggcggcg tccgagatcg
     2281 caaggttgcg cacgcccggc tgcacggtgg cggtgcggtt gaggggatcg atctccagga
     2341 tgcggttaaa tttcgccagc gacagcagca cgccgtcttc gagcggcagc gcgccgccgg
     2401 agagtccggt gccggcgccg cgcgcgacca cgggaatatt ttgcccggag cacagccgca
     2461 ggaggcggcc gacctgttcc accgtctccg gcagcgccac gatccagggc agccggcggt
     2521 aggcagaaag cccgtcgcat tcgtaggggc gcaggtcctc ttcctcggtc agcaccgagc
     2581 tacgcggcat gaattcgcgc agcatcgccg cgaaacgctc gcgcgcgatg gacgtaccgg
     2641 tggctttctc tgggatgacg gtggccatgc aggctagggt aaacccggac caccggaggg
     2701 taaagccgga tgtctgttac tcgttttcgg ggcagttcgc ctgcctgtca tccaggctga
     2761 tcgggccgct caatttgatg aaaaccccga gcgacatgag cagcaatgac gcgctggcga
     2821 tgatggccac gggataataa agaaaaccgg ccaagtcctg ctggctgtac ttgatcacca
     2881 tgatcagcga ttcaagcacc agggccacga acacgatact ggcgaagcgc gtgacgccac
     2941 ggcggatgcg ccagacggcg tcggaaggtt ttccgctctt gtcgtattcc tggtgtacca
     3001 cctgcgccag ctcgtacacg gccagcgcga ccacgcccat gtgcagccct ttgaccaggc
     3061 cgctggtgag gtcgccgtcg ctgaggatcg gttggatgaa ctcgatgatc gccgaggagg
     3121 cgtacagcag cgcgaaggac aggaagaaca gggcgaacaa cggcaccagc acgcgtggca
     3181 ctgactgtat gattgcagtc aggcccgtgg ttctttcttc cgtgatcgtc tcatgattct
     3241 cgatggtttc atctttcatc attttctcct ttcgtttcgt caacgggatg ctaagtggcg
     3301 catgggcatg caacaacagt tgccccgatc aatttgctat gattcctgac taacgggcat
     3361 aagcccgcgt ttgttatacg gtcatcgcgc ggtaacgcgt aatttgattt gaacgggttg
     3421 tcatgaaatc tccaaaaccg tatcgcggcc gcttcgcgcc gtcgcccacc ggaccgctgc
     3481 acttcggttc gatggtcgcc gcggtcggaa gtttcatcga ggcgcgacat cgtggcggcg
     3541 actggctggt gcgcatcgag gacctcgacc cgccgcgcga aatgccgggc gcagtcgctg
     3601 acattctgcg tacgctcgaa gccttcggcc tgcactggga cggcgaggtg gtgtatcaga
     3661 gccgtcgcca cgcgctgtat gaagaggcgc tggaaaaact gaaagcgcgc gatgagcttt
     3721 atgcctgcgc gtgcagccgg cgcgaaatcg ccgattcgag cgtgaaaggc gtggacgggc
     3781 tggtttatcc cggcacctgc cgcagcggca tcgccgaggg acgcgcaccg cgcgcctggc
     3841 gcgtgaaagt ggacgggcag aagatcgaat tcaacgacgt ccttcaggga cgcacgatgc
     3901 gcgacatggc ggcggacttc ggcgacttcg tcgtgcggcg cgccgacgga ttctttacgt
     3961 atcagctcgc cgtggtggtg gacgacgccg cgcagggcat caccgaggtc gtgcgtggcg
     4021 cggacctgat cgaatcaacc ccgcggcaga ttcatctgca aaaacttctc ggcctgccga
     4081 cgccgggtta tctccatctc cctgttgccc tcagcaagca gcgcgaaaag ctgagcaagc
     4141 agaccttcgc cgcgccggcc gatgtggccc gccctgcgcc attgttgtgt caggtgctgc
     4201 gctttctggg tcaggaagtc ccgcccgagc tggccgatgc gggcattgag gatttctggc
     4261 gctgggctgt cacccattgg gatgtgaaac gtatcccgcg ggtgtcgggg gtggtcgtgg
     4321 aaacaccaaa cgcacagaac tgacaggtaa tgagcgcggg caaagaagaa cacccatttt
     4381 gtggggggct aaggccggtt gccaggctgc ggcgaaaagc agaatatgtc ctggtagaaa
     4441 aacaatatca gggagtcgta ggccaccagg gagaaacggc atggccacgc agtgcaccca
     4501 tctcgatgcg attcgcgacg tcagccccaa caccagcggc tgcgaggaat gcctgaagct
     4561 cggcgatcgc tgggtgcatc tgcgaatgtg catgacctgc gggcatgtgg gctgctgcga
     4621 ttcctcgaag aacaagcacg cgaccaaaca tttccacgcc acccaacatc caatcatgcg
     4681 ctccctcgag ccgggcgagg attggtactg gtgttacgtg gataacatca tgttaggccg
     4741 ccaatcgaag cggagaacat gagggaaagt cggcaagccc aatgaacaag ccgaatgcgc
     4801 tgaaggggtg cccgcttctg gcaggaattc caccagaaga cctccgtcgc gactgtccga
     4861 cagctcggat tgtggcgatc cggcaccgcg gcacgatcta ccggcaaggc gagcccgggc
     4921 gaactgtctt ttgcgtgctc gatgggcagg tgaccatcgc gagggtcagc catgacggcg
     4981 cgactctcac cacggcggcg ttgggagcgg gggacttctt cggccccgct ctgagtggcg
     5041 ccactgcagc cgaagacacg gcgagggcga agggagctgt ttccatatgg cgagcgccga
     5101 tcgacgagtg gcggcgcctg ttgctgcacc accccgcagt cgcgtgggac tttgtctcgc
     5161 ttctcgcgct gcgccagcgc cagatggaac gcaggctcga aagcttcgcg ttcaaacgaa
     5221 ccgaggcacg gctagcccag actttccgcg agttgtcagg agggttcgcc actcgctgca
     5281 accatggctt cggacaacac cttcgcctca cacaacagga gttggccgac ctcgtgggag
     5341 cgagcagacc ggttgtgagt acgatcctca acaaactccg tgacaaggga gtgctcggat
     5401 acactcgtga atacgtgtgc gttcgccgga tcgaggatat cgagaagctg atcgattcct
     5461 gaacgctctg cgcactgtca tctggagaac agcgaggttc tgcgtggcct gtcagattgc
     5521 gggcacgctc ccgtttc
//
LOCUS       DBNGZE010000156         3761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1412115, whole genome shotgun sequence.
ACCESSION   DBNGZE010000156 DBNGZE010000000
VERSION     DBNGZE010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1412115"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16FV_04020"
     CDS             <1..272
                     /locus_tag="AC16FV_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849265.1"
                     /translation="YPPAELAVSNRLADHEKTPLRHLPQRGLFHGLQVDWKRPALQGD
                     TLKINQINAINGCSDKQLQTSVRISTMLRRDLNPSYGLETLTSLN"
     gene            296..1360
                     /locus_tag="AC16FV_04025"
     CDS             296..1360
                     /locus_tag="AC16FV_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR2849266.1"
                     /translation="MKSKSKKLSQLFALVLAFVLGLGNAMAAEEYGIFERILEASGSF
                     NDTTAALEKALAESKLTLQAKRDLTYTDKVQQARVYVVTSPAYMEAAQGESPDTISAQ
                     ILRIGVYEYGEGKKVHINMGNPTAHAMVFYAGSKNYDKLLVAAKAAGQEIRDIAAKVP
                     GKQVAVQLEPVRTEKALNKFNGDGPAKMMAKFRNWKESQNEVFKDKPENFAAAVARVE
                     NTLRASQDKGVEDSSGWRLISKIPVGANAVYFGITNDYTENKCIRINSDFRSEGKSKD
                     APYPGVDHGPAMPLEVLVINDGKEVKVVQYGEMWRMQLYFWDSGYMAFAKNTLIPSII
                     FSSIDTALTATASAAPAPAK"
     gene            complement(1448..3418)
                     /locus_tag="AC16FV_04030"
     CDS             complement(1448..3418)
                     /locus_tag="AC16FV_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR2849267.1"
                     /translation="MINAQQLKERPRLAVNEFSRRLGGYKRWRDELIENLIEYQTWVE
                     SHGLASGEEDLQLYELIDALRSDKLTIALVAEFSRGKTELINAIFFADYRQRLLPSEA
                     GRTTMCPTELRYDASEPPSLKLLPIETRQTATTIAEYKRSPAAWTVMPLDLDSPRKMA
                     ETFQQTVKTKSISREQAQELGLYHPDIEATAHMLNSDGTVEIPLWRHAIINFPHPMLK
                     QGLVVLDTPGLNSLGTEPELTLSMLPGAQAVLFVLAADTGVTQTDLEVWNHVCPTRNN
                     KGESCLVALNKIDDLWDELSNDNAFATAIARQMQETARTLGISKNQVLAVSAQKGLLG
                     KIKTDYALIERSGLRALEAKLSDDIIPARRELLRERVIHDIGSMVETTSAMIEARLNA
                     VTGQIDELRTLGGENSEAIQDLVTRLRDEKQAYDQTLVSFQNTRAVLSDHIKILLDYL
                     SVESFDDLADKTRRGMSESWTTPGLREAMKALFDGVLDAMEKANKQTQQIRSLVQAIY
                     GKFHSEHGLAKIKPANFSLLPYRSQLQKLYEEAEAFRNSPMLIITEQHFVVQKFFVTL
                     VGRAREIFSDGNAAARSWSKAIMAPILSQVREHKILMDQRLENLKKVHENSESLNDRI
                     AELESSRQNLENQQLVIRNMLRKINQPAPIDP"
     gene            complement(3561..>3761)
                     /locus_tag="AC16FV_04035"
     CDS             complement(3561..>3761)
                     /locus_tag="AC16FV_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HIR2849268.1"
                     /translation="GDCLKVRLTAPPVEGKANEHLIAYLARLFGVPKSQVILERGDTS
                     KHKQLRIRSPRKLPENLDWNAK"
BASE COUNT          759 a          995 c         1129 g          878 t
ORIGIN      
        1 tgtatccccc tgcagagctg gccgtttcca atcgacttgc agaccatgaa aagacccctt
       61 tgcggcattt gccgcaaagg ggtctttttc atggtctgca agtcgattgg aaacggccag
      121 ctctgcaggg ggatacatta aaaatcaatc aaatcaacgc aataaatgga tgttctgaca
      181 agcagttgca gactagcgtg agaatcagca caatgctccg cagggattta aatccatctt
      241 acggattaga aaccttaaca agtcttaact agaggcattt aggaggagat atccaatgaa
      301 gtcaaaatca aaaaagctta gccagctttt cgctttggta ttggcgtttg tattggggct
      361 gggcaatgcc atggcagcag aagagtacgg catttttgag cgtatcctgg aagcgtcggg
      421 ttcgttcaat gacaccacag cggcgctgga gaaggccttg gccgaatcca agctcacgct
      481 gcaagccaag cgcgatctga cctacaccga caaggtgcag caggcgcgtg tctatgtggt
      541 cacttccccc gcctacatgg aagccgccca aggcgaatcg cccgacacca tttcggcgca
      601 gatcctgcgc atcggcgtgt atgaatatgg cgagggcaag aaggtccata tcaacatggg
      661 caatccgacc gcgcacgcca tggtcttcta cgcgggttcc aagaactatg acaagttgct
      721 ggtagctgcc aaagcggcgg gtcaggaaat cagggacatc gcggccaagg tgccgggcaa
      781 gcaggttgcc gtgcagctcg agccagttcg caccgagaaa gcgctcaaca agtttaatgg
      841 cgacggtcct gccaagatga tggccaagtt ccggaactgg aaggaaagcc agaacgaagt
      901 attcaaggac aagccggaga actttgccgc ggcggtggcc cgtgtggaaa acacgctgcg
      961 cgcaagtcag gacaaaggcg tggaggattc ctccggctgg cgtctgatca gcaagatccc
     1021 ggtgggcgcc aatgcggtct acttcggcat caccaatgac tatacggaga acaagtgcat
     1081 ccgcatcaac tccgacttcc gcagcgaagg caagtccaag gatgcccctt atccgggcgt
     1141 tgatcacggt ccggcgatgc cgctggaagt gctggtgatc aatgatggca aggaagtgaa
     1201 ggtcgtgcag tacggcgaga tgtggcgtat gcagctgtac ttctgggatt ccggttacat
     1261 ggcgttcgcc aagaacacct tgatcccgag catcatcttt tcctcgatcg atacggcgct
     1321 gacggccacc gcttctgcgg ccccggctcc cgcgaagtaa tccgtttcat caacggacaa
     1381 accaaggccg gaagggtcac cccttccggc ctttttattt gtctctattt tttctgtgca
     1441 ggcatgctca aggatcaatc ggggccggtt ggttgatctt gcgcagcatg ttgcggatga
     1501 cgagttgctg gttttccagg ttctgacggc tggattccag ttcggcgatg cggtcgttca
     1561 gactttcgga attctcgtga acctttttca ggttttccag gcgctgatcc atgaggattt
     1621 tgtgctcgcg tacctgtgac aggatggggg ccatgatggc cttgctccag ctgcgggcag
     1681 cggcgttgcc gtcactgaaa atctcgcgcg cccggccgac caatgtaacg aagaatttct
     1741 gcaccacgaa gtgctgttct gtgatgatca gcatcggact gttgcggaag gcctcggcct
     1801 cctcgtagag tttttgcaac tggctgcgat acggcaacag cgagaaattg gccggtttga
     1861 tcttggcgag tccatgctca ctgtggaatt tgccgtagat agcctgcacc agactgcgta
     1921 tctgctgggt ctgcttgttg gccttttcca tggcatcgag caccccgtcg aacagcgcct
     1981 tcatggcttc ccgcagaccc ggtgtggtcc atgattcgga cataccgcgg cgcgttttgt
     2041 cggccaggtc gtcgaaggat tcgacgctga ggtaatcgag cagaatcttg atgtgatcgg
     2101 agaggacggc gcgcgtgttc tggaagctga cgagcgtttg atcgtaggcc tgcttttcat
     2161 cgcgcaggcg cgttaccagg tcttggatcg cttcgctgtt ttcaccgccg agggtgcgca
     2221 actcgtcaat ttgcccggtg acggcattaa gcctggcctc gatcatggcg ctggtggttt
     2281 cgaccatgga gccgatgtcg tgaatcacgc gctcgcgcag caattcccgt cttgccggga
     2341 taatgtcgtc cgatagtttg gcttccagcg cccgcaggcc gctgcgctcg atcaatgcgt
     2401 aatctgtttt gattttgccg agcaatccct tttgcgcgga caccgccagc acctggtttt
     2461 tgctgatgcc aagggtgcgc gccgtctctt gcatctgacg cgcaatcgcc gtggcgaacg
     2521 cgttgtcatt actcagttcg tcccacaggt catcgatttt gttcagcgcc accagacagc
     2581 tttcaccttt gttgttccgg gttgggcaga catggttcca gacctcgaga tcggtttggg
     2641 tgacgccggt gtcggccgcc agcacgaaca gcaccgcctg cgcgcccggc agcatgctca
     2701 aggtcagttc cggttcggtg ccgagtgagt tgagcccggg tgtgtcgagc accaccagtc
     2761 cctgcttcag catgggatgc ggaaagttga tgatcgcgtg acgccatagg ggaatctcga
     2821 cggtgccatc gctgttcagc atgtgcgccg tggcttcgat gtcgggatga taaaggccca
     2881 attcctgcgc ttgctcgcga ctgatggatt tggtcttgac ggtttgctgg aaggtctcgg
     2941 ccattttccg gggcgaatcc agatccagcg gcatcaccgt ccacgcggcg gggctgcgtt
     3001 tgtattccgc gatggtggtg gcggtctggc gtgtttcgat gggcaggagt ttcaggctcg
     3061 gcggttcgct ggcgtcgtag cgtaattccg tggggcacat ggtcgtgcgg ccggcctccg
     3121 aaggcagaag gcgctgccga tagtcggcga aaaaaatggc gttgatgagt tcggttttgc
     3181 cgcgcgaaaa ttcggcgacc agggcgatgg tgagtttgtc ggagcgcagc gcgtcgatga
     3241 gttcgtacag ttgcagatct tcttcaccgc tggcgaggcc gtgactttcg acccaggtct
     3301 ggtattcgat gaggttctcg atcagttcgt cgcgccagcg tttatatcct cccagccgcc
     3361 ggctgaattc gttgacggcc agccggggcc gctctttcag ctgttgcgcg tttatcatta
     3421 tattgaatac cccgttaata catatggtta gaatataaac ttaaataaga agtatatcta
     3481 gccccacccc tgccggaagg tgtgcaagtg ggctggcgag ggggtaatcc tggtgagtgg
     3541 gggctgcccg aagaaaaggg ctactttgcg ttccagtcga ggttttcggg gagttttcgc
     3601 ggggaacgga tgcgtaattg cttatgtttg gaggtatcgc cccgttccag gatcacctgg
     3661 cttttcggca cgccaaaaag ccgcgccaga taagcaatca ggtgctcatt ggccttgcct
     3721 tccacgggag gggccgtcag ccgaaccttg aggcaatcgc c
//
LOCUS       DBNGZE010000157         3536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_509647, whole genome shotgun sequence.
ACCESSION   DBNGZE010000157 DBNGZE010000000
VERSION     DBNGZE010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_509647"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="dapF"
                     /locus_tag="AC16FV_04040"
     CDS             complement(<1..370)
                     /gene="dapF"
                     /locus_tag="AC16FV_04040"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR2849269.1"
                     /translation="MPVPFTKMHGAGNDFVVFDGVSRPIQLTPGKIRRLADRHFGIGC
                     DQVLLVERPTASDADFRYRIFNADGGEVEQCGNGARCFVRFVRDKRLTAKDEIAVETL
                     AGMIYPRLEADGAVSVNMGVP"
     gene            complement(419..853)
                     /locus_tag="AC16FV_04045"
     CDS             complement(419..853)
                     /locus_tag="AC16FV_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR2849270.1"
                     /translation="MGIFQNPPDEEIGKLLRRVRTIAVIGLSPRTNRPSHGVAAQMQR
                     FGYRIIPVRPAVDSVLGERAYASLREVPDKFDLVDVFRAPEYVDAIVEACIALGVPAI
                     WLQEGVVNVPAAERARQAGLTVVMDRCVYREYLRLFGPVPHP"
     gene            complement(928..2193)
                     /gene="lysA"
                     /locus_tag="AC16FV_04050"
     CDS             complement(928..2193)
                     /gene="lysA"
                     /locus_tag="AC16FV_04050"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR2849271.1"
                     /translation="MNPFSYRDRQLYAENVRLADIAQQVGTPCYVYSRASLEAQFRAF
                     VEALRGRDHLVCYAVKANSNLAVLNLLARLGSGFDIVSGGELERVLAAGGDPAKVVFS
                     GVGKTRDEMRRALAAGIYCFNVESGAELEVLNEVAGELGKPVPVALRVNPDVDPATHP
                     YIATGLKQSKFGIDIAQAEAAYARARSLPNLRVTGIACHIGSQLTDLAPVVEALDRVL
                     GLANTLLDKGFALQHLDLGGGLGIRYNEEQPPAPGDYIRAILERLAAKGGRGQKLRLV
                     FEPGRAIVGNTGLLLTRVLYLKHGSEKNFAVVDAAMNDLLRPALYEAWHDITPVAVDA
                     PRTPRVYDVVGPVCESGDFLGHDRELGIEAGDLLAVVSAGAYGAVMSSNYNTRPRAPE
                     VMVDGGKFHVVRRRETVAELFASETVLPK"
     gene            complement(2210..2404)
                     /gene="lptM"
                     /locus_tag="AC16FV_04055"
                     /gene_synonym="yifL"
     CDS             complement(2210..2404)
                     /gene="lptM"
                     /locus_tag="AC16FV_04055"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF025012.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR2849272.1"
                     /translation="MNLIGNRLIRGRYRQVMLLGMTFAVMFLAACGKKGPLYLPDQAA
                     APDKTGMQAVSPTAAQPGKK"
     gene            complement(2451..>3536)
                     /locus_tag="AC16FV_04060"
     CDS             complement(2451..>3536)
                     /locus_tag="AC16FV_04060"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR2849273.1"
                     /translation="MGKRILVLGGNGTFGRRISQAIAAMPSVECVVGGRHPPKAREFS
                     EVNATTIAVNVRDPANLRRALDGVFAVVNTVGPFQAQDYGVAETCAGMGVHYVDLADA
                     RAYVDGITRLNRRAQQRNCLIVSGASTVPAVSAALVDMLAPEFDRISDIHTCISPGNK
                     NPRGVATVQAILSYTGSSFRIKENGRWRYAFGWSESEKVVFPAPVGKRRVYLCDVPDL
                     DIFPTRFGAQTVTFRAGLELNLFNRGLALLGRMRRWGWIKNLTGWAPGLIAASHLFRG
                     FGGVAGGMRVLVRGRKNDEEIQHAAFLIARDANGPAIPCSPSVALIRKWVERGVPDTG
                     AMPCVGLLTWDEIRAELVSHDIVLVRI"
BASE COUNT          696 a         1180 c         1055 g          605 t
ORIGIN      
        1 gcggtacgcc catgttgacg ctgacggcgc cgtccgcttc caggcgcggg taaatcatgc
       61 cggccagcgt ctcgaccgcg atctcgtcct tggccgtgag ccgcttgtcg cggacgaaac
      121 gcacgaaaca gcgcgcaccg ttaccgcact gctccacctc accgccgtcg gcgttgaaaa
      181 tccggtagcg gaaatcggcg tcgcttgccg tgggccgttc caccagcagc acctgatcgc
      241 agccgatgcc gaagtgccgg tcggcgagac ggcggatttt tccgggcgtg agttgaatcg
      301 ggcgggagac gccgtcgaac accacgaagt cgttgccggc gccgtgcatc ttggtaaagg
      361 gaacgggcat gacggcaagc ttaccggttg gcgcgcggca aataaaccgc gagcgtgatc
      421 acggatgcgg caccggcccg aacagacgca aatactcccg atagacacag cgatccatga
      481 ccacggtcag accggcctgc cgcgcccgct ccgccgcggg gacattcacc acgccttcct
      541 gcaaccagat cgccggcacg cccagggcga tgcacgcctc gacaatcgcg tccacgtatt
      601 ccggcgcacg aaaaacatcc accagatcga atttgtccgg cacctcgcgc aacgacgcat
      661 aggccctctc gcccagcacg gagtccaccg ccgggcgcac cgggatgatg cgataaccga
      721 agcgctgcat ctgcgccgct acgccgtggc tcggacggtt ggtgcgcggc gagagaccga
      781 tgacggcgat ggtgcgcacg cgccgcagca attttccgat ttcctcgtcc ggcggattct
      841 ggaaaattcc catgcgatgt cattccataa ggcgcgcgga gaaaaacaaa ttcagttccg
      901 gaatctccac aagctctgcg agaggattta tttcggcaac acggtctcgg atgcgaacaa
      961 ctccgcgacg gtctcgcgcc ggcgcacgac atggaacttg ccaccgtcca ccatcacctc
     1021 gggtgcacgc gggcgggtgt tgtagttcga gctcatcacg gcgccgtagg cgccagcgga
     1081 aaccaccgcc agcagatcgc cggcctcgat gcctagctcg cggtcgtgcc cgagaaaatc
     1141 cccgctctcg cacaccgggc ctacgacatc gtatacgcgc ggggtgcgcg gtgcgtccac
     1201 cgccaccggc gtgatgtcat gccatgcctc gtacaacgcc gggcgcaaca ggtcgttcat
     1261 ggcggcgtcc accacggcga aatttttttc cgatccgtgc ttgagataca gcacgcgcgt
     1321 gagcagcaga ccggtgttgc cgacgatcgc gcgaccaggc tcgaacacca gccgcagttt
     1381 ctggccgcgg ccgcccttcg ctgccagccg ctccagaatg gcacggatat agtcgcccgg
     1441 cgccggcggt tgttcctcgt tataacggat gccgagcccg ccgccgagat cgagatgttg
     1501 cagggcgaaa cccttgtcga gcagcgtatt ggcgagaccg aggacgcgat ccagcgcctc
     1561 gactacgggc gccaggtccg tgagctggga gccgatgtgg caggcaatgc ccgtcacacg
     1621 caggttcggc aacgatcgcg cccgcgcata ggccgcttcg gcttgcgcga tatcgatgcc
     1681 gaacttgctt tgcttgaggc cggtggcgat gtacgggtgc gtggccgggt ccacgtccgg
     1741 gttgacgcgc agcgccaccg gcaccggctt gcccagctcc cccgccacct cgttcaacac
     1801 ttccagctcc gccccggatt ccacgttgaa gcaataaatg ccggccgcca gcgcgcggcg
     1861 catttcatcg cgtgtcttgc cgacaccgga gaacaccacc tttgcgggat cgccgcccgc
     1921 agcgagcacg cgctcgagtt cgccgcccga aacgatgtcg aagcccgaac ccaggcgcgc
     1981 gagcaggttc aacacggcaa gattggagtt agccttgacg gcgtagcaaa cgagatgatc
     2041 acgccccctc aacgcttcaa cgaaggcgcg gaattgcgcc tcaagactcg cacgcgagta
     2101 gacgtagcaa ggcgtgccaa cctgctgcgc gatatcggcc agtcgtacat tttcggcata
     2161 cagttgccga tcccggtagc tgaatggatt catgatggaa ataatggaat tatttttttc
     2221 cgggttgcgc cgccgtcggg ctgacggcct gcataccggt tttgtccggt gccgcggctt
     2281 gatccggcaa atacaaaggt cctttcttgc cgcaagcagc cagaaacatc acggcaaacg
     2341 tcatgcccag cagcatgacc tgccggtacc gtccccgaat taaacgattt ccgatcaagt
     2401 tcatggcgta agcttacctt gcctgttcgc accgcatcca gtggcggcac tcagattcgc
     2461 accaagacga tatcgtgact gaccaactca gccctgatct cgtcccaagt gagcaggcca
     2521 acgcagggca tggccccggt gtccggcacg ccgcgctcga cccatttgcg aatcaacgcc
     2581 acggaaggac tgcatggaat cgccggaccg ttggcgtcgc gcgcgatcag gaaggcggca
     2641 tgctggattt cttcatcgtt cttccggccg cgcaccaaca cacgcatgcc gcccgcaaca
     2701 ccgccgaaac cacgaaaaag atggctggcg gcaatcaacc ccggcgccca gcccgtcaga
     2761 ttttttatcc acccccagcg ccgcattcgc cccagcaggg cgaggccacg gttgaacaga
     2821 ttcagttcaa ggccggcgcg aaaggtcacc gtttgcgcgc cgaagcgcgt cgggaagatg
     2881 tcgaggtccg gcacatcgca caggtacacg cgtcgcttcc ccaccggcgc ggggaaaacc
     2941 actttttccg actcgctcca gccgaaggca taacgccagc gtccattctc cttgatgcga
     3001 aacgaactgc cggtataact taaaatcgcc tgcactgtag caacgccgcg tggattcttg
     3061 ttgcccggcg aaatgcaggt gtggatgtcg ctgatgcgat cgaattcggg cgcgagcatg
     3121 tccaccagcg ccgccgacac cgcgggcacg gtgctggcgc cgctcacgat aaggcagttc
     3181 ctctgctgcg cgcggcggtt caggcgcgtg atgccatcga cgtaggcgcg cgcatcggcg
     3241 agatccacat agtggacgcc catgccggcg caggtttccg ccacgccgta gtcctgcgcc
     3301 tgaaacggcc ccacggtatt gaccacggcg aatacgccgt cgagcgcgcg ccgcaggttc
     3361 gccgggtcgc gaacattcac cgctatcgtc gtggcattga cctcggaaaa ttcacgggct
     3421 ttcggagggt gacgtccacc gacgacgcac tcgacggatg gcatggcggc gatggcctgg
     3481 ctgatgcgcc ggccgaaagt gccattccct cccaggacaa gaatccgctt tcccat
//
LOCUS       DBNGZE010000158        10576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1203947, whole genome shotgun sequence.
ACCESSION   DBNGZE010000158 DBNGZE010000000
VERSION     DBNGZE010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1203947"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..64
                     /locus_tag="AC16FV_04065"
     ncRNA           <1..64
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_04065"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            complement(85..696)
                     /gene="coaE"
                     /locus_tag="AC16FV_04070"
     CDS             complement(85..696)
                     /gene="coaE"
                     /locus_tag="AC16FV_04070"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232372.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR2849274.1"
                     /translation="MLRIGLTGGIGSGKSTIASLFAVRGVPVIDTDKIAHGLTEPDQE
                     TYNEIVRAFGNTILDENCRIDRNKLRERVFDSADERHCLETILHPRIRVIVRDKLAAL
                     EAPYVIVVVPLLIESGFIDLVDRVLVVDALENVQIQRTATRTGLSESEIRKIMSAQAS
                     RAQRLQQANDVIENNGDRKQLEAEVERKHQWYLSLAATTKKTN"
     gene            complement(705..1589)
                     /locus_tag="AC16FV_04075"
     CDS             complement(705..1589)
                     /locus_tag="AC16FV_04075"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR2849275.1"
                     /translation="MSNISAFFASYPAAFPWIAGLFGLAIGSFLNVAIYRIPIMLERK
                     WRNQCQEILRPNKKVPDTAKRFDLMVPGSRCPHCGHAITALENIPVLSFLWLRGKCSA
                     CAKSISWRYPLVELLTGVLTAMVAWHLGYGMAALTGLVLTWSLIALSFIDFDRQLLPD
                     DITLPLLWAGLLVNIFAIFTSLPSAVIGAAGGYVFLWLVYQIFKLVTGKEGMGYGDFK
                     LFAALGAWLGWQSLPLIILLSSLVGAIVGIAFILFFGHDRRMPIPFGPFLCVAGWVAL
                     LWGDALTRYYLQFARLSL"
     gene            complement(1641..2867)
                     /locus_tag="AC16FV_04080"
     CDS             complement(1641..2867)
                     /locus_tag="AC16FV_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR2849276.1"
                     /translation="MAEAAIKKPKFDTFTWEGVDKQGKEVKGIMEAASVAYVNATLRR
                     QGVNPVKVAKQRKAAFKFKKKITTKDIAVFTRQLATMLTSGIPIAQSFDIVGKGHENP
                     SMQEVIMSIKQDVESGTALTQALGKHPLYFDALYCNLVQAGEQAGILDGILDKVATYK
                     EKIEAIKGKIKSALFYPTAVIVVAFIITAVLLVFVIPQFESLFAGFGADLPAMTKMVI
                     DLSKAFQEWWWAIIGSIVGTVVFVSYSYKRSEKMQHSLDRILLKAHIIGEIVKKATIA
                     RFARTLATMFAAGVPLVEALDSVAGASGNRVYYEGTMAIKSDVSTGMQLQASMNATGL
                     FPNMVVQMVAIGEESGELDKMLSKVADFYEAEVDDAVAGLSSLLEPIIMAFLGVVIGG
                     LVVAMYLPIFKLAATV"
     gene            complement(2871..4592)
                     /gene="pilB"
                     /locus_tag="AC16FV_04085"
     CDS             complement(2871..4592)
                     /gene="pilB"
                     /locus_tag="AC16FV_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HIR2849277.1"
                     /translation="MATASTATNLSGLALRLVRDNLLSPTDAERIQTEALSKKTSFVT
                     QLVESKKLDSATIAKVASEDFGIPLYEINSLDLEMAPVKLVDEKIIRRHHVLPLFKRG
                     TRLFVAVADPTNLQALDEIKFHTGLGTEPILVEEAKLATIIEKSLDAQDTSLADLSSD
                     DSLENLEIVAGEEDKDSPGIGGEEGVNDAPVVKFVNKVLMDAINRGASDIHIEPYEKT
                     YRVRYRQDGMLHEVANPPIALSARIAARIKILSRLDIAERRVPQDGRIKMRISKNRAI
                     DFRVSTLPTLWGEKIVMRILDPTSATLGVEKLGFEDFQKELFLEAVHRPYGMVLVTGP
                     TGSGKTVTLYTAVNILNTPDTNISTAEDPVEINLSGVNQVNINDKAGLNFASALRAFL
                     RQDPDVILVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPSTLTRLANMGVPPFNIA
                     SAVNLIVAQRLARRLCAHCKKPVDIPKPALLKAGFREEDLSGLTVYGPVGCDACNGGY
                     KGRVGIYQVMPVTEEIGAIIMRGGNQLDIEKQARKEGVPDLRQAGLMKVKAGVTSMEE
                     VEAATNE"
     gene            5043..5465
                     /locus_tag="AC16FV_04090"
     CDS             5043..5465
                     /locus_tag="AC16FV_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR2849278.1"
                     /translation="MSKLKGSKTEGNLKAAFAGESQANRRYLYFAAKADVEGYNDVSA
                     VFRSTAEGETGHAHGHLEYLEQCGDPATGLPIGATSDNLKAAIAGETHEYTDMYPGMA
                     KAARDEGFAEIADWFETLAKAERSHANRFQKALDTLGK"
     gene            5595..6941
                     /locus_tag="AC16FV_04095"
     CDS             5595..6941
                     /locus_tag="AC16FV_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR2849279.1"
                     /translation="MSQPTEGRREGSLQAPTRHPLEWCEADFYNEEALMEELERVFDI
                     CHGCRRCVSLCNSFPSLFDLVDNSPTLEMDGVKKEDYWQVIDHCYLCDLCFMTKCPYV
                     PPHEWNVDFPHLMLRAKAVKFKKGDVKKRDKLLTNTDMVGKLAGVPVVVQTVNAANRS
                     GAMRKILDKVLGVHPEAILPEYHSKTLRKRIKKRDGSGLAAMPAGRTRGKVVLFTTCY
                     GNYNEPHLGEDLVAVFEHNGIPVTLAEKERCCGMPKLELGDLEAVARAKDVNIPVLAK
                     LVDEGWDIVAPVPSCVLMFKQELPLMFPDDPEVAKVKQAIFDPFEYLMLRHKEGRLKT
                     DFKKGLGSVSYHVPCHLRVQNIGLKTREVLSLVPNTKIDTIERCSGHDGTYAVKTEFH
                     KISMKICKPVVNRVEHAKPEHFSSDCPMAGHQIENGLQGDMKPEHPMSLLRLAYGI"
     gene            6960..7265
                     /locus_tag="AC16FV_04100"
     CDS             6960..7265
                     /locus_tag="AC16FV_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA];
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HIR2849280.1"
                     /translation="MANEGYHEPENELSDETRDMHRAIISLMEELEAVDWYNQRVDAC
                     KDDELRAILAHNRDEEKEHASMMLEWIRRRDKRFDKELRDYLFTKNPIVDLEHQAKE"
     gene            7310..7897
                     /locus_tag="AC16FV_04105"
     CDS             7310..7897
                     /locus_tag="AC16FV_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR2849281.1"
                     /translation="MKKLNREDLFSLEKYAEVRQQFRAQVMAHKQNRQVAIGPNATLY
                     FEDRMTMQYQVQEMLRIERIFEAEGINDELAAYNPLIPDGSNWKATFMVEFPDVEERR
                     AALKRLKGVEKHVWVRVAGFEPVRPHADEDLEREDEEKTSSVHFLRFELTPDMVRAVR
                     QGAAVAMGIDHPAYTHQVDPVPAATRDALARDLSD"
     gene            7991..8560
                     /locus_tag="AC16FV_04110"
     CDS             7991..8560
                     /locus_tag="AC16FV_04110"
                     /inference="COORDINATES: protein motif:HMM:NF020331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HIR2849282.1"
                     /translation="MQGATRFIVWGVGGKRIFFGRLLLSLALFAAIYPLHAKRDQYGT
                     GPEYFDFDESKVEKWKESEVILPAYPKDENLLAIPLLATDTLKIYVDRVSLSRGADRV
                     ARFSLVVESPSGARSVFYDGLRCETREYKTYAIGSPEHVFTPVKDAAWRRIPQPAMNA
                     FRYHLYRHYICDAHASARTPEELVRLLTQ"
     gene            8598..10490
                     /gene="parE"
                     /locus_tag="AC16FV_04115"
     CDS             8598..10490
                     /gene="parE"
                     /locus_tag="AC16FV_04115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR2849283.1"
                     /translation="MSAAYDSSAIEVLTGLEPVRRRPGMYTQTDRPNHLAQEVIDNSV
                     DEAIAGHCSAIDVTLHSDGSLSVADNGRGMPVDKHKEDGVSGVEVILTRLHAGGKFSN
                     KNYIYSGGLHGVGVSVVNALSHKLEVTVRRDGKEYHMSYADGQKTSELKAVGKAEKNV
                     TGSIIRFWPDAQFFDTVKFHVPRLKHVLRAKAVLCPGLYISFTNEADKKDNEEWHYED
                     GLKDYLKTQLEGWEYLPDEPFVGHHKGDDGEVDWAIVWLPAGGEPIMESYVNLIPTTQ
                     DGTHINGFRAGLTDAMREFCEFRDLLPRGVKLAPEDIWGKSSYVFSLKMRDPQFSGQT
                     KERLTSREAASFVQGVTKDAFSLWLNHHVADAEKIAALAIESAQERQRADKKVARKKI
                     GTGPALPGKLADCVAQDLSRTELFLVEGDSAGGSAKQARDRDFQAIMPLRGKILNTWE
                     VDPAEVLGSQEVHDIAVALGVDPGSSNLEGLRYGKVCILADADSDGAHIATLLCALFV
                     RHFRPLVADGRVCVAMPPLYRIDVGKEIHYALDDEEKTGILKRIESNGSRAKPMVTRF
                     KGLGEMNPDQLRETTMDPKTRRLVQLEIQSGDSTNKLLDMLLGKKRASDRKNWLEKNG
                     DKAEVI"
BASE COUNT         2352 a         2939 c         3044 g         2241 t
ORIGIN      
        1 gtacactccg gttcctgcgc tccgtcctca cccggctgac ggccgctcgc gacggttctg
       61 agacggcttg ctcaaccaac cttcctaatt ggtttttttc gttgtggccg cgagtgacaa
      121 ataccactgg tgtttgcgtt ccacctcggc ctcaagctgt ttccggtcgc cattattctc
      181 gatcacgtca ttcgcctgtt gcagtcgctg cgcccggctt gcctgcgccg acatgatttt
      241 gcgtatttcc gattcgctga gcccggtgcg ggtggcggtg cgctgaattt gtacattttc
      301 caaggcgtcc acgaccaaga cacgatccac aagatcgata aaaccggact cgatcaacag
      361 tggcacaaca acgataacat agggcgcctc gagcgcggcc agcttatccc gaacgatgac
      421 gcggatgcgc gggtgcaaga tcgtctcgag acagtggcgt tcatcggcac tgtcaaagac
      481 ccgctcgcgc aatttattcc ggtcgatccg gcagttttca tccaggatcg tgttaccaaa
      541 ggcgcggaca atttcattat acgtttcctg gtcgggttcc gtcaggccat gcgcgatctt
      601 atcggtatca atcaccggta ccccgcgcac ggcgaacagc gacgcgatgg tggatttgcc
      661 actgccaatg ccgccggtca ggcctatgcg caacatggtg ccggctaaag actgaggcga
      721 gcgaactgca ggtagtaacg cgtcaaggca tcgccccaca ggagcgcgac ccatccggcc
      781 acgcacaaga acgggccgaa gggtatgggc atgcggcgat catgaccaaa gaacaggatg
      841 aaagcgattc ccacgatcgc gccgaccagt gaagacaaca agatgatcag gggcaggctt
      901 tgccatccca gccatgcgcc cagtgcggca aagagtttga agtcaccata gcccatgcct
      961 tccttgccgg ttaccagttt gaaaatctga tataccagcc agaggaaaac atatccaccg
     1021 gcggcaccga taactgccga tggcaatgaa gtgaagatgg caaaaatatt caccaacagt
     1081 cccgcccata gtaacggcag agtaatgtcg tccggtagca gctgccggtc aaaatcaatg
     1141 aagctgagtg cgatcagact ccaggtcagc accagaccag tcagggctgc cattccatat
     1201 cccaaatgcc aggcgaccat tgcggtcagg acacctgtca gcagttcgac aagagggtaa
     1261 cgccaggaaa tggatttggc gcaggccgaa cattttccac gcagccagag aaaactcaga
     1321 accgggatgt tttcgagcgc ggtgatggcg tgtccgcaat gtgggcaacg cgagccaggc
     1381 accatcagat caaaccgttt ggcggtgtcc ggaacttttt tgtttggcct gagaatttcc
     1441 tgacactggt tgcgccactt ccgctccagc atgatcggaa tacgatatat agcgacattc
     1501 aggaaacttc cgatcgccag tccaaaaaga ccggcgatcc agggaaatgc ggcagggtag
     1561 ctggcgaaga aagccgagat attactcatc gggcggcatt atgcccgaat ggttataaaa
     1621 agcagcaccg ggaacggagc ttaaactgta gcggcaagct tgaagatcgg cagatacatg
     1681 gcgacgacca gaccgccgat gacgacgccg agaaaggcca tgatgatggg ttcaagcaga
     1741 ctggaaagac ctgccacggc gtcatccact tcggcttcat agaaatcggc gactttactc
     1801 agcatcttgt cgagttcacc cgattcctcg ccgatggcga ccatctgcac caccatgttg
     1861 gggaaaagcc cggtggcgtt catcgacgcc tgcaattgca taccggtgct gacatcagat
     1921 ttgatggcca tcgtgccttc ataataaacg cggttgcctg aggcacccgc gacggaatcc
     1981 agcgcttcca ccagcggcac gccagcggcg aacatcgtgg ccagcgtgcg cgcgaagcgg
     2041 gcgatggtgg cttttttgac gatctcgccg attatgtgtg ctttgagcaa gatgcgatcc
     2101 agcgagtgtt gcattttctc ggagcgctta tacgaatagc tgacgaacac caccgttccg
     2161 acgatcgaac cgataatggc ccaccaccat tcctggaagg ccttggaaag atcgatgacc
     2221 atcttggtca tggccggcaa atcagcgcca aaaccggcaa acaggctttc gaactgtgga
     2281 atgacaaata ccagcaagac agcggtgatg atgaaggcca ccacgataac cgcggtcggg
     2341 tagaacaggg cggacttgat cttgccttta atggcttcga tcttttcctt gtaggtggcg
     2401 accttgtcca gaatgccgtc caggataccg gcctgctcgc cggcctgtac cagattgcag
     2461 tacagggcat caaaataaag tggatgtttg ccaagggcct gggtcagcgc agtccccgac
     2521 tccacgtctt gcttgatgga catgattact tcttgcatgg acgggttttc atgccctttg
     2581 ccgacgatgt cgaaggactg ggcaatgggt atgcccgagg tcagcatggt tgcgagctga
     2641 cgggtaaaga ccgcaatatc cttggttgtg attttcttct tgaacttgaa agccgccttg
     2701 cgctgcttgg ccaccttgac ggggttgact ccctgccggc gcagcgtggc attcacgtaa
     2761 gcgacgcttg ccgcttccat gatgcctttg acttcctttc cctgtttgtc gacgccttcc
     2821 cacgtaaaag tgtcgaattt gggtttcttt atcgccgctt ctgccatggt ttactcgttg
     2881 gttgccgcct cgacttcttc catgctggta acgccagcct tgaccttcat caggcctgcc
     2941 tgccgcagat cgggaacgcc ttccttcctg gcctgctttt cgatatccag ctgattgcca
     3001 ccgcgcatga tgatggcgcc gatttcttcc gtcaccggca tgacctgata aatacccaca
     3061 cgtcctttat agccgccatt gcaggcatcg caacccacgg gaccataaac agtgaggccg
     3121 gaaagatctt cttccctgaa gccggccttc aacaaggccg gcttgggaat atcaacgggc
     3181 ttcttgcaat gcgcgcacag tcgccgagcc agacgctggg cgacgatcag gttgactgcc
     3241 gaggcgatgt tgaatggtgg cacgcccatg ttggcgaggc gggtcagcgt ggacggcgcg
     3301 tcgttggtgt gcagggtcga gagcaccata tggcccgtct gcgccgcctt gatggcgatc
     3361 tcggccgttt ccagatcacg gatttcgccg acgagaatga cgtccggatc ctggcgcagg
     3421 aaggcgcgta acgccgaagc gaaattgagt cccgccttgt cgttgatgtt cacctgattg
     3481 acgccggaca ggttgatttc caccggatct tcggccgtgg aaatattggt gtccggcgta
     3541 ttgaggatat tcaccgccgt gtagagggtg acggtcttgc cgctgccggt cggtccggta
     3601 accaacacca tgccgtaggg gcgatggaca gcctcgagga acagttcttt ctggaaatct
     3661 tcgaacccca gtttttccac gccgagcgtg gcagaggtcg ggtcgagaat acgcatcacg
     3721 attttctcgc cccataatgt cggcagggtg ctgacgcgga agtctatcgc gcggttcttc
     3781 gagatgcgca tcttgatgcg gccgtcctgc ggaacgcgcc gttccgcgat atcgagcctg
     3841 gaaagaatct tgatacgcgc ggcaatgcgt gccgacagcg ctattggcgg gttagcgact
     3901 tcgtgcagca tgccatcctg acggtaacgt acacgatagg ttttttcgta aggctcgatg
     3961 tggatatccg aggcgccgcg gttgatggcg tccatcagaa ccttgttgac aaatttaacg
     4021 actggagcgt cattgacacc ttcctcgcca ccaattccag gactgtcctt gtcttcctcg
     4081 cctgcgacga tttcgaggtt ttccaggctg tcatcactgg atagatcggc cagactcgta
     4141 tcttgcgcat ccagtgactt ctcgatgatg gtggcaagtt tggcttcctc gaccaggatc
     4201 ggttccgtgc cgaggccggt gtgaaacttg atttcatcca gtgcctgcag attggtggga
     4261 tcggcgacgg cgacgaaaag ccttgtgccg cgcttgaaga gcgggagtac atgatgacgc
     4321 cggatgatct tctcatcgac cagcttgacc ggggccattt ccaggtcgag cgaattgatc
     4381 tcatacaacg gtataccgaa atcctcggag gccaccttgg cgatggtggc gctgtcgagt
     4441 ttcttgctct cgaccagctg tgtgacaaat gaggtttttt tggaaagtgc ttccgtttgg
     4501 atgcgttccg cgtccgtcgg actgagcaga ttgtcgcgca ccagcctcag cgcgaggccg
     4561 ctcaggttcg ttgccgtgct tgcagttgcc atcgtgatgg attcaggtaa gcgtcactgc
     4621 tattgcgctg aatcttttca atatagtctt agatatacgg atgcgccaaa tcatttttcc
     4681 tcctgttttc cggtagttat atgcgtctgt cataaatttg ttctctcaat ttgccggtgg
     4741 cgcgcgattg acaaccgcgg tgaaaattgc cggaggaaat agtgccctgt gttggctgtc
     4801 atcgtgctca agagggttgc ggatgacacc aaacctaaga atagccagat tccccttaat
     4861 ctccattgcg gatttgcatt caccacaaca cggctgttga catgagtttt tcatctattt
     4921 agaattattc taaatcgaga agtgtttgat tgatttatgt tgttctagat caaagttttc
     4981 gatttaaata acttttaaat tcatcaagtt atcgaaattt tactgaaacg aggaaattgc
     5041 ttatgtcaaa actcaaagga agcaagaccg agggtaatct gaaggctgca tttgccggcg
     5101 aatcccaggc caaccgccgt tacctgtatt tcgccgccaa ggccgacgtg gaaggctata
     5161 acgacgtgtc ggcggtattt cgctccaccg cggaaggcga gaccggccac gcgcacggac
     5221 acttggagta tctggagcag tgcggcgacc cggccaccgg ccttccgatc ggcgcgacct
     5281 cggataacct gaaggccgcc attgccggtg aaacccacga atacaccgat atgtatccgg
     5341 gcatggccaa ggccgcgcgc gacgaaggct ttgccgaaat cgccgattgg ttcgaaacgc
     5401 tggccaaggc cgagcgttcg cacgccaacc ggttccagaa ggccctcgat accctgggca
     5461 aataagtcgc agttatgaac gacggggtcg gtcctccggc cccgttgttt caccttggcc
     5521 agccttgatt gctatcaaag ctgtccgttt cataaagagg aagatgttct gagttcatca
     5581 gacagatttc aatcatgagc caaccgactg aaggcagacg tgaaggcagt ctccaggcgc
     5641 cgaccagaca tcccctggag tggtgcgaag ccgattttta taacgaagag gcgctcatgg
     5701 aagaactcga gcgcgtcttc gacatctgtc acggctgtcg tcgctgtgtc agtctgtgca
     5761 attctttccc gtcgttattc gatctcgtcg acaattcacc gacactggaa atggacgggg
     5821 tcaagaagga agattactgg caggtcatcg atcattgcta tctctgcgat ctgtgtttca
     5881 tgaccaaatg cccgtatgtg ccgccgcatg aatggaacgt cgattttccg cacctgatgc
     5941 tgcgcgccaa ggcggtgaag ttcaagaaag gtgatgtcaa aaagcgcgac aaacttctca
     6001 ccaatacaga tatggtcggc aaactggccg gtgtcccggt ggtggtgcag acggtaaacg
     6061 ccgcgaaccg ttccggcgcg atgcgcaaga tactggataa ggtattgggc gtgcatccgg
     6121 aagcgatatt accggagtat cacagcaaaa ccctgcgcaa gcgtataaag aagcgtgatg
     6181 gcagtggctt ggcagccatg ccagccggac gtacacgcgg caaagttgtg ttgtttacca
     6241 cctgttacgg taactataac gaaccgcatc tcggcgaaga tctggtcgct gttttcgaac
     6301 acaacggcat tcccgtgacg ctggccgaga aagaacgttg ctgcggcatg cccaagcttg
     6361 agctgggcga tctcgaggcc gtggcgcgcg ccaaggatgt caatatcccg gtgctggcga
     6421 aattggtgga cgagggctgg gacattgtcg cgccggtgcc gtcctgcgtg ctgatgttca
     6481 agcaggagtt gccgctcatg tttcccgacg atcccgaggt cgccaaggtc aagcaggcca
     6541 ttttcgaccc attcgaatac ctgatgctgc gccacaagga aggaaggctg aaaaccgatt
     6601 tcaagaaagg actgggttcc gtgtcctatc atgtgccctg tcatctgcgc gtgcagaata
     6661 tcggcctcaa gacccgcgag gtgctgagtc tcgttcccaa cacgaagatc gacaccatcg
     6721 agcgttgctc cgggcacgac ggtacctacg cggtgaaaac cgagttccac aagatttcca
     6781 tgaaaatctg caaacccgtg gtcaaccggg tggaacatgc caagccggag catttttcca
     6841 gcgattgccc catggccggg caccagatcg aaaatggatt gcaaggcgac atgaagccgg
     6901 aacacccgat gtcgctgctg cgactggcct acggtatttg aagcgtaaag gaaactagca
     6961 tggcaaatga aggttatcac gagccggaga atgaattgtc ggacgagaca cgcgacatgc
     7021 atcgcgccat catctcgctg atggaagaac tcgaggcggt ggactggtac aaccagcggg
     7081 tggacgcctg caaggacgac gagctgcggg cgattctggc gcacaaccgc gatgaggaaa
     7141 aggagcatgc ctcaatgatg ctggaatgga tccgccgccg cgacaaacgc ttcgacaagg
     7201 agctgcggga ttacctgttt accaagaatc cgatagtgga cctggaacac caggccaagg
     7261 agtaacaacg atgaaccaga tttcaggaca agctagcgag agtgttttca tgaaaaagct
     7321 gaaccgcgag gatctttttt ctctcgagaa gtatgcggaa gtgcgtcagc agttccgcgc
     7381 acaggtgatg gcgcacaagc aaaatcgcca agtcgccatc ggcccgaatg ccacgctcta
     7441 cttcgaggac cggatgacca tgcagtatca ggtgcaggag atgcttcgta ttgagcgcat
     7501 tttcgaggcc gaaggcatta acgatgaact cgccgcctac aaccctctta ttccggatgg
     7561 cagcaactgg aaagcgacct tcatggtcga gtttccggac gtcgaggaac gccgtgcggc
     7621 cctgaagcgt ctgaaagggg tggaaaaaca tgtatgggtg cgcgtggccg gtttcgagcc
     7681 ggtacgcccg cacgcggacg aggacttgga gcgcgaggat gaggaaaaaa cctcctccgt
     7741 ccattttctg cgtttcgagc tgacgcccga catggtcagg gcggtcaggc agggcgccgc
     7801 ggtggcgatg ggcatagacc acccggccta tacccatcag gtcgatcccg ttccggcggc
     7861 gacacgcgac gccttggcgc gggatctgtc ggactgacga acaccatctc cccgcgctgt
     7921 caaccgcgat tgaataatcg cgacgcggtt tctcgtgcgc ggcgcgcatc tgctgtatac
     7981 tgcgcgcccg atgcaaggag ctacccgttt tatcgtctgg ggcgtcggag gaaaaagaat
     8041 tttcttcggt cgcctgctgc tgtccctggc gctgtttgcc gccatctatc cgctccacgc
     8101 caagcgtgat caatacggga ccggaccgga atatttcgat ttcgacgaaa gtaaggtgga
     8161 gaagtggaag gaaagcgagg taattctccc cgcttacccc aaggacgaga atcttctggc
     8221 gattcccctg ctggcgacgg acaccctcaa aatttacgtt gaccgcgtat cgctttcacg
     8281 tggcgccgat cgcgtcgcgc ggttttcgct ggtggtggaa agcccgtcgg gcgcgcgcag
     8341 cgttttctac gacggcctgc gctgcgaaac ccgggaatac aagacctacg ccatcggaag
     8401 cccggaacat gtattcacgc cggtcaagga cgctgcgtgg cgcagaatcc cgcagcccgc
     8461 gatgaatgct ttccgttacc atctttaccg tcattacatc tgcgacgcgc atgcctcggc
     8521 tcggaccccc gaggaacttg tacgcctgtt gacccagtga tatcaatgaa ctttatttca
     8581 cgattatctg actatccatg agcgctgcct acgattcctc cgcgattgaa gtcctgaccg
     8641 gtcttgagcc ggtgcgtcgc cgtccgggca tgtacaccca gacagaccgg cccaatcatc
     8701 tcgcccagga agtcatcgac aacagcgtgg acgaggccat cgccggacat tgcagtgcca
     8761 tcgatgtcac gctgcattcc gacgggtcgc tctcggtggc ggacaacggc cgcggcatgc
     8821 cggtggacaa gcacaaggag gatggcgtgt cgggcgtcga ggtgattctt acgcgcctgc
     8881 atgcgggcgg gaaattctca aacaaaaatt acatctattc cggcggtttg cacggcgtcg
     8941 gcgtatcggt ggtcaacgcg ctttcgcaca agctggaagt cacggtgcgg cgtgacggca
     9001 aggaatatca catgtcctac gccgacgggc agaagacctc tgaactgaaa gcggtcggca
     9061 aggcggagaa aaacgtcacc ggcagcatca ttcgtttctg gcccgatgcc cagtttttcg
     9121 acacggtgaa attccatgtg ccgcgcctga agcacgtgct gcgtgccaag gcggtgctat
     9181 gtccgggtct gtatatcagc ttcacgaacg aagctgacaa gaaggacaac gaagagtggc
     9241 attacgaaga tggcctcaag gattatctga aaacgcagct tgagggctgg gaatacctgc
     9301 ccgatgagcc ttttgtcggc caccacaaag gcgatgacgg cgaagtggat tgggccatcg
     9361 tgtggttgcc cgcgggtggc gagccgatca tggaaagcta cgtcaacctg attccgacca
     9421 cgcaagacgg cacgcatatc aacggcttcc gcgccggcct gaccgacgcc atgcgcgaat
     9481 tctgcgagtt ccgtgacttg ctgccgcgcg gggtgaagct cgcgcccgag gatatctggg
     9541 gcaagtcaag ctatgtgttc tcgctcaaga tgcgcgaccc gcaattctcg ggacagacca
     9601 aggagcgcct gacctcgcgc gaggctgcga gtttcgtgca aggcgtgacc aaggatgcct
     9661 tcagtctgtg gctcaatcat catgtggccg acgccgagaa aatcgcggcg ctcgcgatcg
     9721 aatcggcgca ggaacgccag cgcgcggata agaaagtcgc gcgcaagaaa atcggcaccg
     9781 gcccggcctt gcccggcaag ctcgcggatt gcgtggccca ggacctgtcg cgcaccgagc
     9841 tgtttctggt ggaaggcgat tccgcgggcg gttccgccaa acaggcgcgc gatcgcgatt
     9901 ttcaggctat catgccgttg cgcggcaaga ttctgaatac ctgggaagtg gacccggccg
     9961 aggtgctggg ctcacaggaa gtgcacgata ttgcagtcgc gctcggcgtc gatccgggct
    10021 cgagcaatct cgaaggcctg cgttacggca aggtgtgcat tctcgcagac gccgattccg
    10081 acggcgcgca catcgcgaca ctcctgtgcg cgctgttcgt gcgccacttc cggccgctgg
    10141 tggcggacgg ccgtgtgtgc gtcgccatgc cgccgttgta ccgcatcgac gtaggcaagg
    10201 aaattcacta cgcgctcgac gacgaggaga aaacgggaat ccttaaacgt atcgaatcga
    10261 acggctcacg cgccaagccg atggtcacgc gcttcaaggg cctgggcgag atgaatcccg
    10321 accagctgcg cgaaaccacc atggacccta agacgcggag gctggtgcaa ctggaaattc
    10381 agtccggcga cagcaccaat aagctgctcg atatgctgct cggcaagaaa cgcgcgtccg
    10441 accgcaagaa ctggctcgag aagaatggcg acaaggcgga ggttatctag tgccgaccat
    10501 caagaccgcc gttgacgtcg ccgcctcgcc cgaattggtg tggcgcgtgt tgacctattt
    10561 ccccgattac ccgaaa
//
LOCUS       DBNGZE010000159         3003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_301104, whole genome shotgun sequence.
ACCESSION   DBNGZE010000159 DBNGZE010000000
VERSION     DBNGZE010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_301104"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="AC16FV_04120"
     CDS             <1..715
                     /locus_tag="AC16FV_04120"
                     /inference="COORDINATES: protein motif:HMM:NF018652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HIR2849284.1"
                     /translation="RDEALRAAEAARAAGFDNFNLDLMFGLPGQTVEQALADIRTAIQ
                     LGPAHLSAYQLTIEPNTLFHAHPPILPDDDSAWDMQSRLQEGLAAAGYRQYEVSAYAK
                     PGYECRHNLNYWKFGDYLGIGAGAHAKITDATGISRLWKVKQPKEYLCTAGTPQGIGG
                     EERLSRQHIVTEFMMNALRITRGFPSSLFAERTGLPISAVAKTLEQAEQKGLIAWDVE
                     TLHPTDTGFRFLNNLVSLF"
     gene            883..1392
                     /locus_tag="AC16FV_04125"
     CDS             883..1392
                     /locus_tag="AC16FV_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR2849285.1"
                     /translation="MKATQLLILAVIALCSIGTVYAEDEPWYAGVKAGRMKVDLSGYD
                     NAIGVGGMLGYKLNNTLGIEGGYTTTGEDSDVMVGTSQAQWDIDVVAVHLAYRTEGNF
                     YAKGKVGALYQEVSVNLANAGEPKSGGEFTYGVGAGWRPFKDIALELEYTVIESDVSF
                     ISIGINLGI"
     gene            1640..2152
                     /locus_tag="AC16FV_04130"
     CDS             1640..2152
                     /locus_tag="AC16FV_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR2849286.1"
                     /translation="MKKHTKLLVILATAAVLAWATTQLFPPAPSIPPETEFTLLDGTK
                     TTLRALRGRPVLVSFWATTCPPCVEELPDLIQLYRDWHPQGLELIAVAMPYDPPIQVQ
                     EFVKRHNVPYPVAIDVQGKVTSAFGGVPYVPTAFVIAPDGKTELSYTGRLDMAKARRI
                     ISRYLKASGA"
     gene            2178..2597
                     /gene="hemJ"
                     /locus_tag="AC16FV_04135"
     CDS             2178..2597
                     /gene="hemJ"
                     /locus_tag="AC16FV_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR2849287.1"
                     /translation="MLWIKSLHIIFLVTWFAGLFYLPRLFVYHSMASATDREGIERFK
                     VMERKLFFGIMTPGGVLTIVFGLWLWLGYGFAGGWLYAKLALVVVLILYHIYCGKLMI
                     DFKYDRNRHNHVWYRWFNEFPTVIFVVTVILVVVKPF"
     gene            2703..>3003
                     /locus_tag="AC16FV_04140"
     CDS             2703..>3003
                     /locus_tag="AC16FV_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2849288.1"
                     /translation="MSYRIKRTVVLGLLLGVSALAAAESFDRDQAVRHALAHNPDLLA
                     IREQVAAAGGRTRAAEGARLPSLGLSYSAQSSNNPLAVFGGKLNTRSVTTQDFDPA"
BASE COUNT          649 a          896 c          818 g          640 t
ORIGIN      
        1 acgcgatgag gcactgcgcg ccgccgaagc ggcgcgcgcg gcgggcttcg acaatttcaa
       61 tctcgatctc atgttcggcc tgcccggtca gacggtggaa caggcgctgg cggacattcg
      121 caccgccatt caacttggcc ccgcacacct ttccgcctat cagctcacga tcgaaccgaa
      181 tacgctgttc catgcgcatc cgccaatact gcccgatgac gacagcgcct gggacatgca
      241 aagccggctg caggaggggc tggccgcggc cggttaccgg caatacgagg tctcggccta
      301 tgccaagccg ggctatgaat gccggcacaa tctgaactac tggaagttcg gcgactacct
      361 cggcattggc gccggcgccc acgccaagat caccgacgcc acgggtattt cacgcctatg
      421 gaaagtgaaa caaccgaaag aatatttgtg cacagcgggc acgccgcaag gcatcggtgg
      481 cgaggaaaga ctgtcacgtc agcacatcgt gaccgaattc atgatgaacg ccctgcgcat
      541 cacccgtggt tttccatcca gcttgttcgc ggagcgcact ggcctgccta tttctgccgt
      601 ggcaaaaacc ctggagcagg cggaacagaa aggactgatc gcctgggatg ttgaaactct
      661 ccacccgacg gacaccgggt tccgttttct caataatctc gtctcgcttt tctagcgacc
      721 gcaaaagtcg ataacaatac ccacgtacgg tatccgttcg ggccggattg aacggcggga
      781 caaattcatt cacaatgatc gcgcatttca ggcaggatgg tggaaaccgt tttaaagctg
      841 tttttgggct ttccgtcaat aaattcgaaa aggataccaa ttatgaaagc gacacagctt
      901 ctgattcttg cagtcattgc cctgtgctcc atcggtaccg tgtatgcaga ggacgaaccc
      961 tggtatgccg gcgtcaaggc agggcggatg aaagtcgatt tgtcaggcta tgacaatgcc
     1021 ataggcgttg ggggcatgct tggatataaa ctcaacaaca ccttggggat cgagggtgga
     1081 tacacgacca caggtgaaga tagtgatgtg atggttggca catcgcaagc gcaatgggac
     1141 attgacgtag ttgcagtaca cctcgcctat cgtaccgagg gtaatttcta cgccaaaggc
     1201 aaagtcggcg ccctctatca ggaggtttcg gtaaatcttg ccaacgcggg tgagcctaag
     1261 tcgggtggag aattcaccta cggcgttggc gcgggctgga gacctttcaa agacatcgcc
     1321 ctggaactcg aatacaccgt cattgaatcc gacgtcagct tcattagcat tggtataaat
     1381 ctcggcatct aagccaaatc ctttgttgca caaaaaaaca atccggcctc gtggccggat
     1441 tgttttttgc gcatccagcc tgatcgataa caaacaacac tccctgaacg cagcgggctg
     1501 acctgctcat atatcgtcaa gcttgcgctg ctgctattga tcgcttacct tgattgcctc
     1561 ttggaaacca agcgagcggc tatcgttaga cctgccttcc cgctcctttc gcatcatcaa
     1621 tacctgacag atgttacaga tgaaaaagca caccaaactg ttggtcattc tggcaaccgc
     1681 cgcggtgctg gcctgggcga cgacacagtt gtttcctccc gccccgtcca taccgccgga
     1741 aacagaattc accctgcttg atggaaccaa gacaacactg cgcgcgctgc gcggacgccc
     1801 ggtgctggtc agtttctggg ccaccacctg cccaccttgc gtggaagagt tgcccgatct
     1861 catccagctc tatcgcgact ggcatccgca gggactggaa ctgatcgccg tcgccatgcc
     1921 ctacgatccg ccgatacagg tgcaggaatt tgtgaaacgc cataacgtcc cctatcccgt
     1981 cgcgattgat gtgcaaggca aggtgaccag cgccttcggc ggcgtgccct acgtgccgac
     2041 tgcgttcgtc atcgcgccgg acggaaaaac cgaactgagc tacaccggcc gtctggacat
     2101 ggccaaggcc cggcgtatca tctcgcgcta cctgaaagca tccggcgcct gatccgcatc
     2161 gccgccaaaa aaccatcatg ctctggatca aatccctgca catcattttt ctcgtcacct
     2221 ggttcgccgg gctgttctat ctgccgcgtc tgttcgtgta tcacagcatg gcatcggcca
     2281 ctgaccgcga gggcattgag cgcttcaagg tgatggagcg caaacttttc ttcggcatca
     2341 tgactccggg cggagtcctc actatcgtgt tcgggctgtg gttgtggctc ggctacggct
     2401 tcgccggcgg ctggctgtac gccaagctcg cgctcgtcgt tgtcctgatc ctctaccaca
     2461 tttactgcgg caagctgatg atcgacttca aatacgaccg caaccgtcat aaccatgtgt
     2521 ggtaccgctg gttcaatgaa tttccgaccg tgatcttcgt cgtcacggtc attctggtcg
     2581 tggtcaagcc gttctgattt tgacaagaat cattgatttt cagggcacgc gcctgtattg
     2641 atgccgtatc aggactcaca accagggggg cgcgggagga gcaacgatac ctggagggtc
     2701 gcatgtcata ccgaatcaag cgaaccgtcg tgctgggcct cctgcttggc gtcagcgcct
     2761 tggcggcagc cgaaagcttc gaccgcgacc aggcggtgcg tcatgcactg gcgcacaatc
     2821 ccgacctgct ggcgatacgt gaacaggttg ccgccgccgg cgggcgcacc cgggcggcgg
     2881 aaggcgcgcg tctgccgagt cttggtcttt cctacagtgc gcaatccagc aacaacccgc
     2941 tcgccgtctt cggcggcaag ctcaataccc gcagcgtcac cacgcaggac ttcgatcccg
     3001 cgc
//
LOCUS       DBNGZE010000160         2729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_647710, whole genome shotgun sequence.
ACCESSION   DBNGZE010000160 DBNGZE010000000
VERSION     DBNGZE010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_647710"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC16FV_04145"
     CDS             <1..568
                     /locus_tag="AC16FV_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2849289.1"
                     /translation="LLKTISGLMRPRRGEIVFNGAPVAGAPAFDVLRRGIALVPEGRR
                     LFPSLTVEENLLIGGQSRRSGPWTLARIYELFPVLAERRNLPAPALSGGQQQMAAIAR
                     ALMANPQLLLCDEISLGLAPIVVRDIYARLPQIVAEGLSLIIVEQDIVQALRAASHIY
                     CLQEGRVALQAPARELTRETISAAYFGV"
     gene            573..1439
                     /locus_tag="AC16FV_04150"
     CDS             573..1439
                     /locus_tag="AC16FV_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2849290.1"
                     /translation="MQWFNVILQGVLIGGLYAMFAAGLSLIFGVMRLVNIAHGDLIVL
                     AAYVSLMVTQGLGVDPLTSLLLVVPAMAVIGYALQRGLLNRTLGDDLLPPLLVTFGLS
                     IIIQNGLLELFTADSRKLQAGAIEIQTIQLVQGVWIGILPFIQFIVAVIAIAALQMLF
                     YRTALGRAFRATSDDQAVAQLMGLDTRHIFAMAMALSLAVIAIAGVFLAVRANFDPAI
                     GPARLIFGFEAVIIGGLGSMWGTLAGGIILGVSQAIGAQIDPGWQLLAGHIVFLAVLA
                     VRPQGLFPKVSG"
     gene            1436..2452
                     /locus_tag="AC16FV_04155"
     CDS             1436..2452
                     /locus_tag="AC16FV_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2849291.1"
                     /translation="MIVEHATRSSRVGVALIAVMLVVLAFAPYWGDRQMLRLLAEIYT
                     LVALASLWNLLAGYAGLVSVGQQAFVGLGGYLLFALAILVDVHPIAAILIAGPIGALV
                     AIPVAALIFRLRGAYFAIGTWVVAEVFRLLASQISVLGGGSGISLPAAVVIAMAPNRQ
                     MREFETYWLALALTGVILGAIVALLRSRHGLALTAIRDNELAAQSNGVDVWRIKFIVY
                     VLTAFGTAMIGALIFLQKLRISPDTAFSVNEWTAFVIFITVIGGIGRIEGPIIGTVIF
                     FLLRQVLADLGSLYLLMMGAVAIVVMLWAPKGIWGLIVERLGWQALPLQRHLILEKET
                     GGKP"
     gene            2449..>2729
                     /locus_tag="AC16FV_04160"
     CDS             2449..>2729
                     /locus_tag="AC16FV_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR2849292.1"
                     /translation="MSPIIDIYCHIFPDRYFQEMTKVAPKLENIGKRLRGVTKLFDLD
                     ARFKEMDEFGDYRQVISLPNPAIEDIAAPDVGLNLARIANDAMAELCRK"
BASE COUNT          448 a          814 c          831 g          636 t
ORIGIN      
        1 gcttctgaaa accatttctg gcctgatgcg tccccgccga ggcgagattg tattcaacgg
       61 tgcacctgtc gctggtgcac ctgcctttga tgtgctccgc cgcggcatcg cgctggtgcc
      121 cgaaggacgc agactcttcc cctcgctgac agtcgaggaa aatttattga tcggcggcca
      181 gtcgcgcagg tccggcccct ggacgcttgc gcgcatatac gaactgttcc ctgtgctagc
      241 cgagcgacgc aacctgccgg cgcctgcgct ctccggtggc cagcagcaaa tggcagccat
      301 cgctcgcgcg ctgatggcta acccgcaact actcctttgt gacgaaatca gcctcggtct
      361 tgcgccaatc gttgtgcgcg acatctatgc acggctgccg cagatcgttg cggagggcct
      421 gtcactgatc atcgtcgaac aggacatcgt gcaggccctg cgtgctgcaa gccacatcta
      481 ctgtttgcag gaagggcgcg ttgctctgca ggcaccggca cgcgagctga cgcgcgagac
      541 aatttcggcc gcctatttcg gggtgtgacg ggatgcaatg gttcaacgtc atcctacaag
      601 gcgtgctgat cggcgggctc tacgccatgt tcgcggccgg cctgtcgctc atcttcggcg
      661 tgatgcggct ggtcaacatc gcgcatggcg atctgatcgt cctcgccgct tatgtgtcgc
      721 tgatggtgac gcaaggactg ggcgtggatc cactgacttc gctactgctg gtcgtgccgg
      781 cgatggcggt catcggctac gcgctgcagc gcgggctact caaccggact cttggcgacg
      841 acttattgcc gccgctgctg gtgactttcg gcctatccat catcatccag aatgggttgc
      901 tggagctgtt cactgccgac agccgcaagc tgcaggcggg cgcgattgaa atccagacca
      961 ttcaattggt gcagggggtc tggatcggca tcttgccatt catccagttc attgttgcag
     1021 tgatcgccat cgccgcgttg cagatgctgt tctaccggac agcgctcggc cgcgctttcc
     1081 gggctacctc cgacgatcag gcggtggcgc agctgatggg gctcgacacc cgtcacatct
     1141 tcgccatggc aatggcgctg tcgctcgcgg tcatcgctat tgccggtgtt ttcctggcgg
     1201 tgcgcgccaa ttttgatcct gccatcggtc cggcgcggct gatcttcggc tttgaggcag
     1261 tgattatcgg cgggttgggc agcatgtggg gaacgctggc aggtggcatt attttgggcg
     1321 tctcacaggc gatcggcgcg cagattgatc cgggctggca actgctcgcc ggtcacattg
     1381 tattcctcgc cgtcctggcc gtgcggccgc aaggactgtt tcctaaggtg tcgggatgat
     1441 cgtcgagcat gccacccggt cgagccgcgt cggcgtcgcg ctcatcgccg tcatgctcgt
     1501 cgttttggca ttcgcgccct attggggcga ccggcagatg ctgcggttgc tcgcagaaat
     1561 ttacacgctc gtggcactgg caagcctttg gaatctgctc gccggctacg cgggactggt
     1621 ttcagtcggc caacaggctt tcgtcggcct tggcggatat cttttgttcg cgcttgccat
     1681 tttggtggat gtgcatccga tcgcagccat cctgattgcc ggacctatcg gcgcactggt
     1741 cgcgatccct gtcgccgcgc tcattttccg cctccgcggc gcctatttcg ccatcggcac
     1801 ttgggtcgtt gccgaagtgt ttcgcttgct cgcctcgcaa atttctgtcc tcggtggcgg
     1861 ctctggcatc agcctgcctg cagccgtcgt cattgctatg gcgccgaacc ggcagatgcg
     1921 cgaattcgaa acctattggc tcgcgctggc actcaccggc gtcatattgg gtgcgatcgt
     1981 ggcgctgcta cgctcacgtc acggcctcgc gctcacggct atccgtgaca atgaactggc
     2041 ggcgcagagc aatggcgtcg acgtgtggcg gataaaattt attgtctacg tgctcacggc
     2101 cttcggcact gcgatgatcg gtgcgcttat ttttttgcag aaactgcgca tttcgcctga
     2161 taccgcgttt agcgtgaacg aatggacggc cttcgtcatc ttcatcactg tcatcggggg
     2221 cattggccgg atagaaggcc caatcattgg cacggtcatc ttcttcctgc tgcgccaagt
     2281 cttggccgac cttggcagcc tgtacttgct gatgatgggt gcggtcgcca tcgttgtcat
     2341 gctgtgggcg ccgaaaggaa tttgggggct gatcgtcgag cgcctgggct ggcaggcgct
     2401 gccattgcag cgccatttga ttctggaaaa agaaacgggg ggaaagccat gagcccgatc
     2461 atcgacattt attgccacat cttcccggac cgctatttcc aggaaatgac caaggtggcg
     2521 cccaagctcg agaatattgg caagcggctg cgcggtgtaa ccaagctgtt cgatctcgat
     2581 gcgcgcttca aggaaatgga cgagttcggc gactatcggc aagttatttc cttgccgaat
     2641 ccggccatcg aggacatcgc agcgcccgac gtaggcttga accttgcccg cattgcgaac
     2701 gacgcgatgg ccgagctatg tcgcaagca
//
LOCUS       DBNGZE010000161         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1343176, whole genome shotgun sequence.
ACCESSION   DBNGZE010000161 DBNGZE010000000
VERSION     DBNGZE010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1343176"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16FV_04165"
     CDS             complement(<1..308)
                     /locus_tag="AC16FV_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR2849293.1"
                     /translation="MNAQVDNVASPVTFTQRKAGWLQALGDAMQRRRSVIQSIQWTVV
                     AVYLALVVIPAFLPLPLEATHIYDNLTRLAQFAFWGVWWPFVMVSMMLMGRVWCGVFC
                     P"
     gene            complement(335..724)
                     /locus_tag="AC16FV_04170"
     CDS             complement(335..724)
                     /locus_tag="AC16FV_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR2849294.1"
                     /translation="MAQHTRLNSLVKLDDKRDKRRMFIAATALWLAAGAGLAVAEELL
                     TLKLTARNGRFYPETIEVPSGARFKIVIVNEGPGPEEFESENPRREKVLAAGATSFLV
                     FPALKPGVYPFFGEFHPETAKGRIVVK"
     gene            complement(755..>1800)
                     /locus_tag="AC16FV_04175"
     CDS             complement(755..>1800)
                     /locus_tag="AC16FV_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HIR2849295.1"
                     /translation="GEIGNAEGKLFAHFRMGQGDGLTRILPAFGGANATGFHVQGARP
                     DDDATVLLAQAWYQLDVPLPLGGFKPQSRENLSFNFGKMDPFLFFDQNSIADDETTRF
                     LNTAFVHNPLLDAGGDVGVDGFGFTPGLRLAYHNETNRPLTWDASLGVFGAGSGAQFS
                     NSFEKPFVIAQLDTEQRFFGGLAGNYRLYFWRNGQATPYANEADTTTEVHAGWGLSAD
                     QRVGDATTLFGRYGHQTRGKVRFDRALTLGAEFGGSYWSRGADALGVAFGQLNTSADF
                     RNDAPALDANADGTPDFGYTASGSEQIAEVYYRRRINKQFELSPDLQHVSRPAGNGAK
                     DMNLIGLRAQVIF"
BASE COUNT          344 a          564 c          563 g          329 t
ORIGIN      
        1 ggacagaaca cgccgcacca cacgcgcccc atgagcatca tcgacaccat cacgaacggc
       61 caccacactc cccagaacgc gaattgcgcg aggcgcgtga gattgtcgta aatgtgcgtc
      121 gcttccagcg gcaggggtag gaacgcagga atgaccacca gcgcaagata aaccgcgacc
      181 accgtccact ggatggactg gatgacggaa cggcgccgct gcatggcatc gcccagagct
      241 tgcagccatc cggctttacg ctgggtgaac gtgaccggac tggcgacatt atctacctgc
      301 gcattcatgc gtgaatatcc gtccgcctct caaatcactt gacgacgatc cggcccttcg
      361 ccgtctcggg atgaaactcc ccgaagaagg gataaacccc gggcttgagc gcggggaaca
      421 ccagaaacga ggtcgccccg gccgccagca ctttttcgcg gcggggattc tcggactcga
      481 actcctcggg cccgggacct tcgttgacga tcacgatctt gaagcgcgcg ccggatggca
      541 cctcgatggt ctccggataa aagcggccgt tcctggcggt gagcttgagt gtcagcagtt
      601 cctcggccac ggcgagcccg gcgccggcgg cgagccatag cgcggtggcg gcgatgaaca
      661 ttcgacgctt gtctcttttg tcatcaagtt tgaccaggct attcaatctc gtgtgttgtg
      721 ccataaatgc cttcgttgct ataggttcgc ttaatcaaaa tatgacctgc gctcgcaggc
      781 cgatcagatt catgtctttg gcgccgttgc cggcgggacg gctcacatgc tgtaagtccg
      841 gtgacagctc gaactgcttg ttgatgcgcc ggcggtaata cacctcggcg atttgctcgg
      901 aaccgctcgc ggtgtagccg aagtcgggcg tgccgtcggc gttggcatcg agagcgggtg
      961 catcgttgcg gaagtccgcg ctcgtgttaa gttgcccgaa ggcgacgccc agggcatcgg
     1021 cgccgcgact ccagtagctg ccgccgaact cggcgcccag cgtcagcgcc cgatcgaaac
     1081 gcaccttgcc cctggtttga tgaccgtaac gaccgaacag ggtcgtggcg tcgcccacgc
     1141 gctgatcggc actcaacccc cagccggcgt gaacctcggt cgtggtatcg gcctcgttgg
     1201 cgtagggcgt ggcctggccg ttgcgccaga aatacagccg gtaattaccg gcaagaccgc
     1261 cgaagaagcg ttgctcggta tcgagttgcg cgatcacgaa cggcttctcg aagctgttcg
     1321 agaactgtgc gccactgccg gcgccgaaga cgccgagcga ggcgtcccag gtgagcggcc
     1381 gattcgtttc gttgtgatag gcgaggcgca atccgggcgt gaagccgaaa ccatccacgc
     1441 ccacgtcgcc ccccgcgtcg agcagcggat tgtgcacgaa cgcggtgttg agaaaacgcg
     1501 tggtctcgtc gtcagcgatg ctgttctggt cgaaaaacag gaacgggtcc atcttgccga
     1561 aattgaaact caggttttcg cgcgactgcg gcttgaaacc gccgagcggc agcggcacat
     1621 cgagctggta ccatgcctgc gcgagcagca cggtggcgtc atcgtcgggc cgcgctccct
     1681 ggacgtggaa acccgtcgcg ttggcgccgc caaaggcggg caggatgcgc gtcagcccgt
     1741 cgccctggcc catgcggaaa tgcgcgaaca gcttgccttc ggcattgccg atctcgccac
//
LOCUS       DBNGZE010000162         3223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1134957, whole genome shotgun sequence.
ACCESSION   DBNGZE010000162 DBNGZE010000000
VERSION     DBNGZE010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1134957"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="AC16FV_04180"
     CDS             <1..910
                     /locus_tag="AC16FV_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849296.1"
                     /translation="LVAGAWAAASIFWWRYLIFAPALTAMFAAGILLGWIWQRAQTML
                     GRPHPGLRWYQLALVSLMLGLCAISIAAFWPEHWAALRRFHLHINIYGFIGLTAVGTL
                     RVLLPTVAGYTDAVTRKRLQRDLYPMALGALLLAIGSAWWTWLVWPGLALWLLPLARF
                     AVPLLSRWREHIWGWHRPSASLAFAVPGLMLVLMVGSLHALGTLPAGIALPLFFYLFL
                     FPLITGAISYLLPVWVWPARDTPAYESAVRRLAWGSGARALVFFMGGVMAWAGLPNAG
                     YLAGVVLAVFLAQAIWALSARLSAPV"
     gene            995..1177
                     /locus_tag="AC16FV_04185"
     CDS             995..1177
                     /locus_tag="AC16FV_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849297.1"
                     /translation="MASHYTNGNTGYKGIERRKFNRRNVVDRRKEIRWEPNNPNRRQA
                     AGRRATDHLGVNGNKR"
     gene            complement(1474..1803)
                     /locus_tag="AC16FV_04190"
     CDS             complement(1474..1803)
                     /locus_tag="AC16FV_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849298.1"
                     /translation="MSELVVAPMFLPAEPLRKRAPAFDEHGKALSDFMVLIPGLVKKP
                     QHLIQATIEHIQTVFADYKHVVVFAELNLKLSLLWVSVKPVPGVRFQISEALRALIPE
                     ARLVSHI"
     gene            2003..2320
                     /locus_tag="AC16FV_04195"
     CDS             2003..2320
                     /locus_tag="AC16FV_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR2849299.1"
                     /translation="MKAEQRHSIRKNKSLNVLINHDLAYSKHGKVRDISLSGARLEME
                     NEGLVPGTPVEAVLALKEQDEYGLFRVPADVVRMGRDGVALRFGRYDNRTYTALVNLL
                     YGS"
     gene            complement(2317..2937)
                     /locus_tag="AC16FV_04200"
     CDS             complement(2317..2937)
                     /locus_tag="AC16FV_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR2849300.1"
                     /translation="MRTYSIYQKPCPACGTVVSVDTKSCDCGYSFDSSASTDELLSEE
                     QALQEEELFEAYLAARIEQTVATVESARAELAADMSNQRKADKLLRTVQEALALRDER
                     DAQAAKIAQIRESLSSKSDAPSMSAQPTEAFRAQQSAKAEKIMEGFVNTQAKTCPQCH
                     TVLPVTSALCLCGYNFARNDFLLPRAVDSSTRGDIYHSKEDSRSSG"
BASE COUNT          569 a          929 c         1019 g          706 t
ORIGIN      
        1 cttggttgcc ggtgcttggg cggcggcgag catattctgg tggcgttatc tgattttcgc
       61 gcctgcgctt accgccatgt ttgcggcagg catcctgctc ggctggatct ggcagcgggc
      121 gcagaccatg ctgggccgcc cgcacccggg cctgcggtgg tatcagctgg cattggtcag
      181 tctgatgctc ggtctgtgcg cgatttcgat cgcggcattt tggccggaac attgggctgc
      241 tctccggcga tttcatttgc acatcaatat atatggtttc atcgggctga ccgcggtggg
      301 cacgctgcgt gttctgcttc ccaccgtggc gggttacacc gatgctgtca cgcgcaaacg
      361 tctgcagcgc gacctttacc ccatggcgct cggtgcgttg ctgttggcta tcggttcggc
      421 ctggtggacc tggctggtct ggcccgggct tgcgctgtgg ctgttgccgc tagcgcgttt
      481 tgccgtccca ttgctttcgc gatggcgtga acatatttgg ggttggcatc gcccaagcgc
      541 gtcattggct tttgccgtgc cgggcttgat gctcgtgttg atggtgggta gtttgcatgc
      601 cctcggcacg ctcccggccg gtatcgctct gccgttgttc ttttatctgt ttctgtttcc
      661 ccttatcacc ggcgccatca gctatctgtt gccggtgtgg gtttggccgg cacgcgatac
      721 ccccgcctat gaatcagccg tgcggcgcct ggcctggggc agcggcgcgc gggcgctggt
      781 tttcttcatg ggcggtgtca tggcatgggc cggcctgccg aacgcgggtt atctggctgg
      841 ggtggtcctg gcggttttcc tggcacaggc gatatgggcc ttatctgctc ggctttccgc
      901 gccggtttag gccttggcat ggtttctgct ttgccttggg tatatcaaac acaggtcggg
      961 gtagtcgcca gaaccctggc cggacgaggg taccatggca agtcattaca cgaacggaaa
     1021 cacgggttac aaggggatag aacggcgcaa gttcaaccgt cgcaatgtgg tggatcgccg
     1081 caaggaaatc cgctgggaac cgaataaccc caaccggcgt caagccgctg gtcgccgcgc
     1141 caccgatcat ttgggcgtga acggcaacaa gcgctagaag ctgtctcaaa acctgctgcg
     1201 ccctgcgctc ctttcccgct gcgttatccg tctccgcggg aaagtccagg gccgccatcc
     1261 ctggcggccc gttcggcgac acgaacgtcc accggacgtt cgtgacaatt ctgccgggag
     1321 cagaattgga cggcggagcg aagcgacgcc ggccccggag cgcgaagcgc gaaggggcga
     1381 gccccaggga tggggcgagc aaatctccgc ctcaccctca cccggctgac ggccgctcgc
     1441 gacggttttg aggcagcttt tcttctacag aagttaaatg tgtgaaacca gacgcgcctc
     1501 gggaatgagc gcgcgcagcg cttcggagat ctgaaagcgc acgccgggca ccggcttcac
     1561 gctgacccat agcagactca gcttcagatt caattcagcg aataccacca cgtgcttgta
     1621 atcggcgaac acggtctgga tgtgttcgat ggtggcctgg ataaggtgtt gcggtttttt
     1681 caccagcccg ggaatgagca ccatgaagtc gctcaaggcc ttgccgtgct cgtcgaacgc
     1741 cggcgcgcgc ttgcgcagcg gctccgcggg caggaacatc ggtgcgacca ccagttccga
     1801 catgatgtat aacccctggt ttgcaggatt tagcgtatac agcttatccg attatggaaa
     1861 aaaaaccaga atccggcggc ccgccgggta ctgagcggat aagtaccccc taatattgcc
     1921 gtgctttccg tatgccggcg cttgacccgt ccccggcaca atcacatcat gtgacgcatg
     1981 gatcaccaga ccgggggagc agatgaaagc cgagcagcgc catagcatcc gcaagaataa
     2041 atcgctcaat gttctcatca atcacgatct cgcctattca aagcacggga aggtgcgcga
     2101 tatcagtctg agcggggcaa ggctggaaat ggaaaacgag ggccttgttc ccggcacccc
     2161 ggtcgaggcc gtgctcgccc tcaaggagca ggatgaatac ggtttgtttc gcgtaccggc
     2221 ggacgtggtg cgcatgggcc gggatggcgt ggcgctccgg tttggtcgct acgataaccg
     2281 cacctacacc gctctggtga atttgttgta cggcagttag cccgaagacc gggaatcctc
     2341 tttggaatga taaatgtcgc cgcgcgtgga gctgtccacg gcccgcggca gcagaaaatc
     2401 gttgcgtgcg aagttgtatc cgcacaggca cagggcggat gtgacgggaa gcacggtgtg
     2461 gcactgcggg caggttttgg cctgggtgtt gacgaatcct tccatgatct tttcggcctt
     2521 ggcggattgc tgggcccgga aggcctcggt gggttgcgcc gacatggatg gcgcatccga
     2581 tttcgatgac agggattcgc gtatttgcgc aatcttggcc gcctgcgcat cgcgttcatc
     2641 gcgcagcgcc agtgcttcct gcacggtgcg caacagcttg tcggccttgc gttgattgga
     2701 catgtcggcc gcgagttcag cgcgcgcaga ctccaccgtc gccacggttt gctcgatgcg
     2761 tgccgccaga taggcctcga acagctcctc ttcctgcagc gcttgctcct cggaaagcag
     2821 ctcgtctgtg ctggcggagg agtcgaaaga gtagccgcaa tcgcagcttt tggtatcgac
     2881 ggacaccacc gtgccgcagg ccgggcacgg cttctgatat atgctgtagg tcctcatgcg
     2941 gtaatccctg tcaatgtcca gcttcttatg acgtctcctt cccaccaaaa ccaagtatag
     3001 acctcgagcg gttctctgcc gagggaaaac gcccacggtg attgtcagaa tggcgtgccc
     3061 gacaagcgga ttggcggcag gcggcacggt tatacaacga cttacgtccc gcaccggttg
     3121 gcaccatggc agggtttaag tattgtggcg ctgatatgga catggctggc tacgaggaaa
     3181 agaaacagcg cctgatggag gtcctgcgtt cgagccgggg cgg
//
LOCUS       DBNGZE010000163         6077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1691016, whole genome shotgun sequence.
ACCESSION   DBNGZE010000163 DBNGZE010000000
VERSION     DBNGZE010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1691016"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="AC16FV_04205"
     CDS             complement(<1..504)
                     /locus_tag="AC16FV_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015620.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR2849301.1"
                     /translation="MSFFIVSGLSGSGKTIALQALEDIGFYCIDNLPAALLPHFASLA
                     ADMRHNAIVNVAIGLDVRNRAFLADLPKSLEELHARGIHYRIIFLEAEDSILVKRFKE
                     TRRKHPMLDRQTPLVEAIRAEKTLLEPLSFDAALRIDTTRTTPHELRSQVRNFARGED
                     TAGLTLLF"
     gene            complement(505..1587)
                     /gene="hprK"
                     /locus_tag="AC16FV_04210"
     CDS             complement(505..1587)
                     /gene="hprK"
                     /locus_tag="AC16FV_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR2849302.1"
                     /translation="MRRKQRKRSTGATTALKAGPIADPKTTPMVTAQDLFEAESGPLK
                     LKWVAGENGTNRLLEPTTAKFPGMALVGHLNFVHPNRVQVIGEAELAYLARLDKAEHE
                     AAVHNLFTCDKTSVVIVANDKKPTADLIEAANQASLALFSSALPSPVVIDHLQYYLTR
                     ALAPRVTVHGVYMEVMGMGVLITGESGIGKSELALELLSRNHRLIADDAVEFVRVGPD
                     VIVGQCPTLLSDFLEVRGLGILDIRLMFGETAVRHKKKLHLIVRLEAIQGKKMSKIDR
                     LQSKQRTRSILDVDIPEVALFVGPGRNLAVLVEAATRAYILRMWGIDPLEEFMKRHHE
                     HMNSSGAGHAAAPAGDALRKARNKVD"
     gene            complement(1777..3276)
                     /locus_tag="AC16FV_04215"
     CDS             complement(1777..3276)
                     /locus_tag="AC16FV_04215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR2849303.1"
                     /translation="MKQSLDLKLGQHLTITPQLQQAIRLLQLSSIELQQEIQEALESN
                     PLLEENEREDETLETKSETELSTSDSADANGEDSGESSYDEADTLPATDADTAPGEES
                     DAEWEETFEPMTAPTASRSTSDDDFPDIDARNSPPQTLRDHLLWQMQMTPFSDTDKQI
                     ALALIDAVSEDGYLSCKLEEIQQSLGKNHAIEMDEIEAVLHQIQNFDPLGVGARDLAE
                     CLRLQMKVLPPDLPDLKAAIQLATPANLALLASRDYTQLRRNLKLTAEELQQAINLIQ
                     GLNPRPGNSVTSSQAGYVVPDIIVRKFRGVWRAELNPDVSPRLRINRQYEKMIHRGDS
                     SADNRYLQDQLQQARWFIKSLASRNDTLLKVARTIVDRQRAFFEHGAEAMKPLVLHDV
                     AETVSMHESTISRVTTNKYMLTPRGIFELKYFFSSHVTTADGGTCSATAIRSIIKKLV
                     ESETPTKPISDSKIAEILAEQGINVARRTVAKYRESMSIPPSNQRKSLV"
     gene            complement(3448..4251)
                     /gene="lptB"
                     /locus_tag="AC16FV_04220"
     CDS             complement(3448..4251)
                     /gene="lptB"
                     /locus_tag="AC16FV_04220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR2849304.1"
                     /translation="MNDKVRQIKRPRNQRAPVAPATSTAPASVLVAEGLTKEYRGRQV
                     IKDVSLEIRSGEIVGLLGPNGAGKTTCFYMIVGLIPMDGGAIRLDDASIGDLPMHKRA
                     RLGLGYLPQEPSVFRNLTVHENIMAILETRAELPDSERKRILEELMHDLNITARRDTL
                     GISLSGGERRRVEIARALATAPRFVLLDEPFAGVDPISVLEIQQLIRHLQKRGIGVLI
                     TDHNVRETLKICDRAYILSDGRLLTAGTPEDILYNDAVKKVYLGEDFRL"
     gene            complement(4259..4792)
                     /gene="lptA"
                     /locus_tag="AC16FV_04225"
     CDS             complement(4259..4792)
                     /gene="lptA"
                     /locus_tag="AC16FV_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR2849305.1"
                     /translation="MTHHKIISLGPVVMLMLSLLAAVTQAAGREEDEPIRVDARSVEA
                     NEKTGAVVYSGNVVVEQGRLSIQADRIEVTTRKGKTELVRATGKPAKLRQRAEGENGE
                     IRAEADRVDYHVSLKKVDLIGHVSLRRGEDLFTADKLHYDLSSKSLSAAGDEKSDGRV
                     HAVIQPKKPGSEPASNP"
     gene            complement(4798..5232)
                     /gene="lptC"
                     /locus_tag="AC16FV_04230"
     CDS             complement(4798..5232)
                     /gene="lptC"
                     /locus_tag="AC16FV_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR2849306.1"
                     /translation="MILDAKRLAIFATLLLLATGSWWLTHTVAVPEKTFDGKLRHDPD
                     YTIENFNATVMNERGQRRYTLSAVQLVHYGDDGSSELEQPYLIQYREGSEPIHTRADK
                     GWMPKNRSEILLQGNAISARGRDPRGAGGEIRVDKMKILLDS"
     gene            complement(5232..5810)
                     /gene="kdsC"
                     /locus_tag="AC16FV_04235"
     CDS             complement(5232..5810)
                     /gene="kdsC"
                     /locus_tag="AC16FV_04235"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HIR2849307.1"
                     /translation="MNQILTPSFEVKPEVLALAAKVKLLLFDVDGVLTDGRLVIGDDG
                     LEFKAFNSRDGHGIKMVQRNGIAVGIITGRTSRVVKHRVKDLQIRHVYQGCKEKLPVY
                     QKLIKKLKLKPEQTAYVGDDVVDLPIMLRVGLAVAVQNAHPLVKQHAHWITPSIGGYC
                     AAREACDMLLHAHGLYHTEMRRYLAVDKEPFA"
     gene            complement(5813..>6077)
                     /locus_tag="AC16FV_04240"
     CDS             complement(5813..>6077)
                     /locus_tag="AC16FV_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR2849308.1"
                     /translation="VDRHGALVGIFTDGDLRRVLARGIDVYNAKITEVMTRNPKTTRA
                     DRLAVEIVQLMQSLKINGLFVVDDKNHVLGALNMHDLFRAGII"
BASE COUNT         1012 a         1787 c         1972 g         1306 t
ORIGIN      
        1 gaacagcagc gtcagtcccg cggtatcctc gccgcgcgcg aagttgcgca cctgcgagcg
       61 cagctcgtgc ggcgtggtgc gggtggtatc gatgcgcagc gcggcgtcga acgacagcgg
      121 ttcaagcagc gtcttttccg cgcggatcgc ctccaccagc ggcgtctggc gatccagcat
      181 cggatgcttg cgccgcgttt ccttgaaacg cttgaccagg atggaatcct cggcttcgag
      241 gaaaatgatg cggtagtgga tgccgcgcgc gtgcagttct tccaggcttt tcggcaggtc
      301 ggcaaggaag gcgcggttgc gcacgtccag accgatggcc acgttcacga tggcgttgtg
      361 gcgcatgtcc gccgccaggc tggcgaagtg cggcagcagc gccgccggca ggttgtcgat
      421 gcagtagaag ccgatgtctt ccagagcctg gagcgcgatg gtttttccgg aaccggacag
      481 gccgctgacg atgaagaaac tcattcaatc cactttgttg cgcgccttgc gcaacgcgtc
      541 tcccgcgggc gccgccgcat ggcccgcgcc gctgctgttc atgtgctcgt ggtgacgctt
      601 catgaattcc tcgagcggat cgatgcccca catgcgcagg atgtaggcgc gggtcgcggc
      661 ctccaccagc accgccaggt tgcggccggg gccgacgaac agggcgacct cgggaatatc
      721 cacgtcgaga atggagcggg tccgttgttt cgactggagg cggtcgatct tgctcatttt
      781 cttgccctgg atcgcctcca ggcgcacgat caggtgcagt ttctttttgt gccgcacggc
      841 ggtttcgccg aacatcaggc gtatgtccag aatgcccagg ccacgcacct ccagaaagtc
      901 ggacagcagc gtgggacact ggcccacgat cacgtccggg ccgacgcgca cgaattccac
      961 ggcgtcgtcc gcgatcaggc ggtggttgcg cgacagcagt tccagcgcca gttcgctctt
     1021 gccgatgccg gactcgccgg tgatcagcac gcccatgccc atgacctcca tgtatacgcc
     1081 gtgcacggtg acgcgtggcg ccagcgcgcg cgtcaggtaa tactgaaggt gatcgatgac
     1141 gaccgggctt ggcagcgcgg acgaaaacag cgcaagcgag gcctggttgg cggcttcgat
     1201 caggtcggcg gtgggtttct tgtcgttggc gacgatgacg accgaagtct tgtcgcaggt
     1261 gaagaggtta tgcaccgctg cttcatgttc ggccttgtcg agcctggcga ggtaggcgag
     1321 ttccgcctcg ccgatgacct ggacgcggtt ggggtggacg aaattgagat gacccaccag
     1381 cgccatgccg gggaatttcg cggtcgtggg ttcgagcagc cggttcgtgc cgttttcgcc
     1441 tgctacccac ttgagcttga gcggtcccga ctcggcctcg aacagatcct gagccgtgac
     1501 catcggcgtg gttttcggat cggcgatcgg gccggccttc agcgcggtcg tagcacccgt
     1561 ggatcttttg cgttgtttgc gccgcaagca gcggttgcct cgaatagtga cgaaggattc
     1621 ctgcgcgtcg tgtgcggggc gggcagggcg tgcgccattg taacaggttt gccgctaatg
     1681 gaatgaggcc cgctccgcgc ggtcgggagc ggacggtgtc ggcaacggga gaatacaggt
     1741 gcgttcggta gtgtggggtc cgcgatcccg gacgggtcac accagcgatt tgcgctggtt
     1801 ggacggtgga atgctcatgg actcgcggta cttggcgacg gtgcggcggg cgacgttgat
     1861 gccttgctcc gccaggattt cggcgatctt gctgtcgctg ataggcttgg tcggcgtttc
     1921 gctttccacc agtttcttga tgatggagcg aatcgcggtg gccgagcagg tgccgccgtc
     1981 cgcggtggtt acatggctgg agaagaaata cttgagctcg aatatcccgc gtggcgtcag
     2041 catgtacttg ttggtggtga cgcgcgagat ggtggactcg tgcatgctga cggtttcggc
     2101 gacgtcgtgc agcaccagcg gtttcatggc ctccgcgccg tgctcgaaga aggcacgctg
     2161 gcggtccacg atcgtgcgcg cgaccttgag cagcgtgtcg ttgcgggaag cgaggctctt
     2221 gatgaaccag cgcgcctgct ggagctgatc ctgcagatag cggttgtcgg cgctggagtc
     2281 gccgcggtga atcatttttt cgtactggcg attgatgcgc agccgcggcg acacgtccgg
     2341 gttgagttcc gcgcgccaca cgccgcggaa tttccgcacg atgatgtcgg ggaccacgta
     2401 accggcctgc gaagaggtga cggaattgcc cgggcgcgga ttgaggccct ggatgaggtt
     2461 gatcgcctgc tgcaattcct ccgccgtgag cttgaggttg cgtcgcagct gggtgtaatc
     2521 ccggctggcg agcagggcga ggttcgccgg ggtggccagc tggatcgccg ccttcaggtc
     2581 gggcaggtcg ggcggcagca ccttcatttg caggcgcaag cactcggcga ggtcgcgtgc
     2641 gcccacgccg agggggtcga aattctgtat ctggtgcaat accgcctcga tctcgtccat
     2701 ctcgatggca tgattttttc ccagagactg ctggatctct tccagcttgc agctgaggta
     2761 gccgtcctcg ctcacggcgt cgatgagggc cagcgcgatc tgtttgtcgg tgtccgaaaa
     2821 cggcgtcatc tgcatctgcc acaacagatg atcgcgcaac gtctgcggcg gactgttgcg
     2881 tgcgtcaatg tcggggaagt cgtcgtcgga agtggagcgg ctggccgtgg gtgccgtcat
     2941 gggttcgaat gtttcttccc actccgcatc gctttcctca ccgggcgcgg tatcggcgtc
     3001 ggttgccggc aaagtgtcgg cttcatcgta ggatgactcg ccgctgtcct cgccgttggc
     3061 gtcggcggag tcggaggtgg acagctcggt ctcgctcttg gtctccagtg tctcgtcttc
     3121 gcgttcgttt tcctcgagca agggattgct ttcgagcgct tcctggattt cctgttgcag
     3181 ttcgatggag gaaagctgca gcagccggat ggcctgctgc agttgggggg tgatggttaa
     3241 atgttgaccc agtttaaggt caagtgactg cttcataatg ccaaaaaagt tctttgataa
     3301 gcggtccgcc ccgggagcgg tttgtgttgt ctacctagag tttagtccat tttcccggcg
     3361 ggaaggctac ttccgtcgtg caggcacaac cttgaacagc ggctatactt gaatcaaaac
     3421 tttaacatga agaagaatcc gcccgtctca gagacgaaaa tcctcgccca gatacacctt
     3481 cttaacggca tcattataca agatatcctc cggcgtgccg gccgtcagta gccggccgtc
     3541 gctcaggata tacgcgcggt cacaaatctt cagggtctcg cgcacgttgt gatcggtgat
     3601 cagcacgccg atcccgcgct tttgcaggtg cctgatcagt tgctgaatct ccagaaccga
     3661 aatcggatcc acgccggcga agggctcgtc gagcagcacg aagcgcggcg cggtcgccag
     3721 tgcgcgcgcg atctccacgc gccgccgctc gccgccggac aggctgatgc cgagtgtgtc
     3781 gcggcgcgcg gtgatgttga gatcgtgcat caactcctcg aggatgcgct tgcgctccga
     3841 atcaggcagt tcggcgcgtg tctcgagtat ggccatgatg ttttcgtgca ccgtcaggtt
     3901 gcggaacacc gacggttcct gtggcagata acccaacccc agacgcgcgc gcttgtgcat
     3961 cggcaggtcg ccgatggaag cgtcgtcgag gcggatcgcg ccgccgtcca tcggaatcag
     4021 tcccacgatc atgtagaagc aggtagtttt gccggcgccg ttcgggccga gcagcccaac
     4081 gatctcgccg ctgcggattt ccagggaaac gtccttgatg acctggcggc cgcggtattc
     4141 cttggtgagt ccttcggcca cgagcacgct cgcgggcgcg gtggacgtgg cgggcgcgac
     4201 cggcgcgcgc tggttgcggg ggcgtttgat ctggcggact ttgtcgttca tgcgcgcttc
     4261 acggattaga tgcgggctcg ctgcctggct ttttgggttg aatcacggca tgcacgcggc
     4321 catcgctctt ctcgtcgccc gcggcgctca ggctttttga actgagatcg tagtgcagct
     4381 tgtcggcggt gaacaggtct tcgccgcgcc gcaacgacac atggccgatc aggtcgactt
     4441 ttttcaagga gacatgataa tccacgcgat cggcctcggc gcgtatttcc ccgttttctc
     4501 cttcggcgcg ctggcgcaac ttggccggtt ttccggtggc gcgcacgagt tcggtcttgc
     4561 ccttgcgcgt ggtgacttca atgcgatccg cctggatgga caggcggccc tgttccacca
     4621 cgacgttgcc gctgtatacc accgcgccgg ttttttcatt ggcctccacg gagcgggcat
     4681 cgacgcgaat cggctcgtct tcctcccggc cggcggcctg cgttacagcc gccagaagcg
     4741 acaacatgag catgacaacc ggtcccaggg aaataatttt gtggtgagtc atgggtgtta
     4801 cgagtcgagc aggattttca tcttgtccac ccggatttcc ccgccggcgc cgcgcgggtc
     4861 gcgcccccgc gcagaaatgg cgttgccttg cagaagtatt tcgctcctgt ttttcggcat
     4921 ccatcccttg tcggcgcggg tatggatcgg ctccgaaccc tcgcgatact gaatcagata
     4981 aggctgttcc agctccgagc tgccatcgtc gccgtaatga acgagctgga cggcggagag
     5041 ggtatagcga cgctgcccac gttcgttcat gaccgtggcg ttgaaattct cgatggtgta
     5101 atcgggatcg tgacgcaatt tgccgtcgaa ggttttttcc ggaacggcca ccgtgtgcgt
     5161 caaccaccac gagccggtcg cgagcaacag cagcgtggcg aagatggcga ggcgtttggc
     5221 gtccaggatc atcaggcgaa cggctccttg tcgacggcaa gataacgccg catctcggtg
     5281 tgatacagcc cgtgggcgtg cagcagcatg tcgcacgcct cgcgcgccgc gcagtaacca
     5341 ccgatgctcg gcgtgatcca gtgggcgtgt tgcttgacca gcgggtgcgc gttctgcacc
     5401 gccaccgcca ggcccacccg cagcatgatc ggcagatcca ccacgtcatc gccgacatac
     5461 gcggtctgct ccggcttgag cttcagcttt tttatcagct tctggtacac cggcagtttt
     5521 tccttgcagc cctgatagac atgcctgatc tgaagatcct tgacgcggtg cttgaccacg
     5581 cgcgaggtgc gtccggtgat gatgccgacg gcgatgccgt tgcgctgaac catcttgatg
     5641 ccatgcccgt cgcgcgaatt gaaggccttg aattccagtc cgtcgtcgcc gatcacgagt
     5701 cggccgtcgg tgagcacgcc atcgacgtcg aacagcagca gtttcacttt cgccgccagc
     5761 gccagcacct cgggtttgac ttcgaatgac ggcgtcagaa tctggttcat ggtcaaatga
     5821 ttccggcgcg aaacaggtca tgcatgttga gtgctcccag cacatgattt ttgtcgtcca
     5881 caacgaacag gccgttaatt ttcaacgatt gcatcagctg gacaatctcg accgccaggc
     5941 ggtcggcgcg cgtggttttg gggttgcgcg tcatgacctc ggtgattttg gcgttgtaca
     6001 catcgatgcc gcgcgccagc acgcgccgca gatcgccgtc ggtgaagatg cccaccagcg
     6061 cgccgtgacg gtccacc
//
LOCUS       DBNGZE010000164         2355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1757103, whole genome shotgun sequence.
ACCESSION   DBNGZE010000164 DBNGZE010000000
VERSION     DBNGZE010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1757103"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..580
                     /locus_tag="AC16FV_04245"
     CDS             146..580
                     /locus_tag="AC16FV_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR2849309.1"
                     /translation="MLIAALLAAFLVILGTFTHYEVLRMLSAYVPRIHIRPRARLLVV
                     LLGTFSGHLLEISFYALAFFYLRDHFDLGTFGGQFADSFSTYLYFSAEAYTSVGLGDI
                     YPLGPLRLITGIEALNGLLLIGWSASFTYLAMQKFWNIRERD"
     gene            641..997
                     /locus_tag="AC16FV_04250"
     CDS             641..997
                     /locus_tag="AC16FV_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018923238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR2849310.1"
                     /translation="MLGGINSALGVMLGAFGAHGLKAKIPAEMLAVYQTGVHYHLFHA
                     LGLLAVGIVATQVADSIYLRWSGWLMLFGIILFSGSLYVLSVSGLRWLGMVTPFGGVA
                     FIASWIVFVIAIAKAA"
     gene            1235..>2355
                     /locus_tag="AC16FV_04255"
     CDS             1235..>2355
                     /locus_tag="AC16FV_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR2849311.1"
                     /translation="MRGIVMQDVLNISGRASFVRRVVPVISLSFGIIVVSLLLTLGAM
                     ELLSVARAYVAGEGLWSKAQRDAVYSLTLYVQTRDPKHFERYRQALSIPLSDQQARIE
                     MDRPDFDYTKAYSGLLGGDNHPDDIPGMIRLYRCCAEYSYFKRAVSIWKEGDAYIAQL
                     QHLGSDLHAEISSGRSSPKRIEAILNQVRAVDAGVKPLEDAFTTTLGEAVRWLRSMLA
                     WTIFSTISFLIVLGVYLSSRILAQARQTEENYGLLADAFAHTADGIMILDSNRRIVAV
                     NHAFTTITGHAPEEVVGQMFVRPQSVKIPGPTIYAVWEDVRSAGRWEGEVWNVHKNGT
                     VFPMRLSLSAVYDRRRDTVGHYVAVFNDISPYKANEERL"
BASE COUNT          445 a          637 c          681 g          592 t
ORIGIN      
        1 aacaatcact tcaacttcgc tccctacggt cgccggacgc tcttatcgtc gcgccggtta
       61 agtgagcgtt gtgcacggat gacaacataa aaataattct ctgacagcaa ctaatttcgt
      121 cccggcgggt catagctggg acattatgct gatcgcggca cttctcgcgg cttttctggt
      181 aatcctgggt accttcacgc actacgaagt gttgcggatg ctctcggcct acgtgccgcg
      241 tatccacatc cggccacggg cgaggctgct ggtggttttg ctgggaacct tctccggtca
      301 tctgctggag atcagttttt atgcgctggc ctttttctat ctccgggatc atttcgatct
      361 gggaactttt ggtggccagt ttgccgatag tttttccact tatctgtatt tctcagccga
      421 ggcctatacc tcggttggtc tgggcgacat ctatccgctg ggacctctgc gtctgattac
      481 cggcatcgag gcgctcaacg gcttgttgct catcggctgg tcggcgtcgt ttacctatct
      541 ggccatgcaa aaattctgga atatcagaga gcgggattag tgcgccgact cgcttgatcg
      601 tatactcctt cctgtgtctc agaccgcaaa attgttccta atgctgggcg gcatcaattc
      661 cgcgctgggg gtcatgctcg gcgcgttcgg tgcgcatggg ttgaaagcga aaatcccggc
      721 ggagatgctg gcggtgtacc agacgggcgt gcattaccat ctgtttcacg cgctcggatt
      781 attggccgtt ggtatcgtgg cgacgcaggt cgcggattca atttatctca gatggtccgg
      841 atggctgatg ctcttcggca tcattctttt ttccggcagt ctttatgtgt tgagcgtgag
      901 cgggttgcgc tggctgggaa tggtcacgcc tttcggcggc gtggcgttta ttgcctcatg
      961 gattgtgttt gtaatcgcga tcgcgaaggc ggcctgatgc agcttggcaa atcgttggcg
     1021 taaaaagcga caaaagttcc ggccctctca aaaaacaccg cgtttttcct gcataattct
     1081 tgtgcaattg gtaccactga taaatcccca ataaccgtgt gtcagtaatt tcctgtcgct
     1141 cccggatcgg cggcctaaaa ttaaactgaa gattcaaaaa acaccgacgt ctgatggatc
     1201 aggcggaatt tcgtgccggt gatccggtca gtgaatgcga gggatcgtaa tgcaggatgt
     1261 gttgaacatt tctgggcgcg ccagttttgt gcgacgggtc gtgcccgtca tcagtctctc
     1321 ttttggcatc attgttgtct ctctattgtt gacgctgggt gcgatggagt tgttgtccgt
     1381 cgcgcgcgcc tacgtggccg gcgagggtct gtggtcaaag gcgcagcgcg acgcggtata
     1441 ttcgctcaca ctctatgtcc agacgcgcga tcccaaacat tttgaacgtt atcgacaggc
     1501 gctttcaatc ccgttgagcg atcagcaggc gcggatcgag atggacagac ccgacttcga
     1561 ttacaccaag gcctattccg ggttgctggg aggcgacaac catcccgacg atatccccgg
     1621 catgattcgc ctgtaccgct gttgcgcgga gtattcctac ttcaagcgcg cggtgtcgat
     1681 atggaaggaa ggcgacgcgt acatcgccca gcttcagcat ctgggcagcg atctgcacgc
     1741 cgaaatttcc tccggaagat cttccccgaa acgtatcgag gcgattctca atcaggttcg
     1801 cgccgttgac gcgggtgtga agccgctgga ggacgccttt accacgacgc tgggcgaggc
     1861 ggtacgctgg ctcaggtcca tgctggcgtg gaccattttc tccaccatct cattcctgat
     1921 cgtgctgggt gtttacctgt cctcgcgtat tctggcgcag gcacgccaga ccgaagaaaa
     1981 ttatggcctt ctggccgacg catttgcgca taccgcggat ggcatcatga ttctggacag
     2041 caaccggcgc atcgtcgcgg tcaatcacgc gttcacgacg atcaccggcc acgcgcccga
     2101 ggaagtggtc ggtcagatgt ttgtccgccc ccagtcggtg aagattcccg gcccgacgat
     2161 ttatgccgtg tgggaggatg tgcgctccgc gggccgttgg gagggcgagg tctggaacgt
     2221 gcacaaaaac ggaaccgttt ttccgatgcg cttgagcctg agcgcggtgt acgaccggcg
     2281 gcgcgacacg gtcggccatt acgtcgcggt gtttaacgac atctcaccgt acaaggccaa
     2341 cgaggaacgg ctcaa
//
LOCUS       DBNGZE010000165         2387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_93562, whole genome shotgun sequence.
ACCESSION   DBNGZE010000165 DBNGZE010000000
VERSION     DBNGZE010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_93562"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC16FV_04260"
     CDS             complement(<1..334)
                     /locus_tag="AC16FV_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR2849312.1"
                     /translation="MQDTVSFELIYLAIPLSCLAGALLAGLLGWKIGRAGAHSVTILG
                     VAVAFALSAFVVLPDVLAGNTFNGPVYTWGISGGIPLEVGFLIDPLTALMMAVVTFVS
                     LCVHIYTIG"
     gene            complement(338..643)
                     /gene="nuoK"
                     /locus_tag="AC16FV_04265"
     CDS             complement(338..643)
                     /gene="nuoK"
                     /locus_tag="AC16FV_04265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR2849313.1"
                     /translation="MIPLSHYLILGAILFSLSVVGIFLNRKNLVILLMAIELMLLAVN
                     LNFIAFSHYLGDIAGQVFVFFILTVAAAESAIGLAILVVVFRNRNSINVEDLSSLKG"
     gene            complement(676..1281)
                     /locus_tag="AC16FV_04270"
     CDS             complement(676..1281)
                     /locus_tag="AC16FV_04270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR2849314.1"
                     /translation="MEFKQLIFYFFGAILVFAAAMVITVRNPVKAALFLVLAFVSAAG
                     LWLLLQAEFLAIALVLVYVGAVMVLFLFVVMMLDINLVRMREGFGEYLPIGGLVAVLL
                     VLEMSIILSTDQFGLKQMPDPGSLPADYSNTRELGRLLYTVYAYPFEIAAGILLVAIV
                     AAIALTQRRRKDSKYQDPSRQINIDPRERVRIVKMRAEKKS"
     gene            complement(1319..1810)
                     /gene="nuoI"
                     /locus_tag="AC16FV_04275"
     CDS             complement(1319..1810)
                     /gene="nuoI"
                     /locus_tag="AC16FV_04275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR2849315.1"
                     /translation="MLKTVKRYLNTFLLFELLKGLMVTGRHLFMRKFTIQYPEEKTPM
                     SPRFRGLHALRRYPNGEERCIACKLCEAVCPAAAITIESEVRADGTRRTTRYDIDLFK
                     CIYCGFCEESCPVDSIVETRFFEFHFENRGEHVIHKDQLLAMGDKYEKQLAEDRATDA
                     KYR"
     gene            complement(1826..>2387)
                     /locus_tag="AC16FV_04280"
     CDS             complement(1826..>2387)
                     /locus_tag="AC16FV_04280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR2849316.1"
                     /translation="GVLMAAGSLNLREIVLAQSGGLHQWYLLPLFPLFLVYLISGVAE
                     TNRAPFDVAEGESEIVAGFHVEYSGMAFAIFFLAEYANMILISALCAVMFLGGWLSPF
                     EGILSAKFLAVPVVGTMLGNGVHWLLLKISFILFFFLWFRATFPRYRYDQIMRLGWKV
                     FIPVTLVWIGVMGGVMFLTPWGFIFH"
BASE COUNT          570 a          744 c          668 g          405 t
ORIGIN      
        1 agccgatggt gtagatgtgc acgcacagcg acacgaaggt gacgaccgcc atcatgagtg
       61 ccgtcagcgg gtcgatcagg aacccgactt ccagcggaat gccgccggaa atgccccagg
      121 tatacaccgg cccattgaag gtgttgcctg ccaacacgtc cggcaggacg acgaaggccg
      181 acagcgcaaa ggccacggcc acgccgagga tcgtcacgct atgcgcgccg gcgcgtccga
      241 ttttccagcc gagcaggccg gccagcagcg ctcccgccag acaggacagc ggaatcgcca
      301 gatagatcag ctcgaaggag acggtgtctt gcattgtcta acctttcagg ctgctcaggt
      361 cttcaacatt aatactgttc cggttgcgga acaccaccac caggatagcc agaccaatag
      421 ccgactcggc cgcggccacg gtcaggatga aaaacacgaa aacctgtccg gcgatgtcgc
      481 cgagatagtg cgagaacgcg atgaaattca ggttcaccgc cagcagcatg agctcgatgg
      541 ccatgagcag gatgaccaga ttcttgcggt tgagaaaaat accgacgacg ctcagggaaa
      601 acaggatggc cccgagaatc aggtaatgcg acaacggtat catcgatccc cttccccttt
      661 tgatgctgtc atcgctcagc tcttcttttc cgcgcgcatc ttgacgatac gcacgcgctc
      721 gcgcgggtca atgttgatct gccgtgacgg gtcctggtat ttgctgtcct tgcggcggcg
      781 ctgggtcaac gcgatggccg caacgatggc caccagcaga atgccggccg cgatctcgaa
      841 cggataggcg tagaccgtat acagcagcct tcccagctca cgcgtgttgc tgtaatccgc
      901 gggcagcgag cccggatcgg gcatctgctt cagcccgaac tggtcggtgc tcaggataat
      961 gctcatctcc agcaccagca gcaccgccac cagcccgccg atcggcaggt attcgccgaa
     1021 accttcgcgc atgcgcacca ggttgatgtc gagcatcatg accacgaaca ggaacagcac
     1081 catgaccgcg ccgacgtaca ccagcaccag cgcgatcgcg agaaattccg cctgcaacag
     1141 cagccacagc ccggcggcgc tcacaaacgc caacaccagg aacagcgccg ccttcaccgg
     1201 attgcgcacg gtgatcacca tggcggcggc gaacaccagt atcgcgccga agaagtaaaa
     1261 aatgagttgt ttaaattcca taaattattc ttgtaaccgc gatgaatacg ggtttttcct
     1321 acctgtattt cgcatccgtc gcgcgatctt ccgcgagctg tttttcatac ttgtcgccca
     1381 tcgccagcag ctggtccttg tgaatcacat gctcgccgcg attctcgaaa tgaaactcga
     1441 aaaaccgggt ttcgacgatg gaatccaccg gacaggactc ctcgcagaac ccgcagtata
     1501 tgcacttgaa cagatcgata tcgtaacgcg tggtacggcg cgtgccgtcg gcgcgcacct
     1561 ccgattcgat ggtgatcgcc gccgccgggc acaccgcctc gcacagcttg caggcgatgc
     1621 agcgttcctc gccgttcgga taacggcgca gggcgtgcag gccgcggaaa cgcgggctca
     1681 tcggcgtctt ctcctccgga tactgtatcg tgaacttgcg catgaacaga tgacgcccgg
     1741 tcaccatcag ccccttcagg agctcaaaga gcaggaatgt gttcagataa cgtttgacgg
     1801 tcttgagcat ggcgcaccct tccactcagt ggaagataaa cccccaaggc gtgagaaaca
     1861 tgacgccgcc catcacgccg atccacacca gcgtcaccgg gatgaacacc ttccagccaa
     1921 ggcgcatgat ctggtcgtaa cgatacctcg gaaacgtggc gcggaaccac aggaaaaaga
     1981 aaagaataaa actgatcttg agcaacagcc agtggacgcc gttgccgagc atggtaccga
     2041 ccacgggcac ggccaggaac ttggcggaca aaattccctc gaacggcgac agccagccac
     2101 ccagaaacat gaccgcgcac agggccgaaa tcaggatcat gttggcgtat tcggccagga
     2161 agaaaatggc gaaggccatg ccggaatact cgacgtggaa cccggcgacg atctcggact
     2221 cgccctcggc cacgtcgaac ggggcgcggt tggtctcggc cacgccggaa atgagataca
     2281 ccaggaacag cgggaacagc ggcaggaggt accactggtg caaaccgcca ctctgtgcca
     2341 gcacaatctc acgcagattc aggctgccgg ccgccatcag cacgccc
//
LOCUS       DBNGZE010000166         3119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_857471, whole genome shotgun sequence.
ACCESSION   DBNGZE010000166 DBNGZE010000000
VERSION     DBNGZE010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_857471"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="AC16FV_04285"
     CDS             <1..1530
                     /locus_tag="AC16FV_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR2849317.1"
                     /translation="QTLQHITEVKHVAREEAQTMGFHVAEEESQTGMRLAEDGMVEIP
                     KWRHAIINIPHPLLQQGLVILDTPGLNALGAEPELTLNMLPSAHAVLFILAADAGVTK
                     SDIDVWHDHIGVRKDSPNKGRLVVLNKIDGLWDELRDWDEVQREIDRQIRETAKHLAI
                     PEQNVFPVSAQKALLGKVKKDTKILERSRILALEQALGGEIVPAKREIVRDNISGDME
                     DVIKAMRLIISQRTKNVYEHMDELGGLSGKNMDVIEHMMKKVKADKEVFEKSLQRFQA
                     TRSIFSQQTNVLYTHLNLKNLDSQIAQTKKEMETSLTTAGLKSCMEGFFKHAQDTMEE
                     ASKQAQEIKAMMEGVYKKFQEEHGLGNIKPGGFSVTRYLREIKRLQERHDQYMKGMSL
                     VFTEQRALTRKFFESSVAKVRAIYKMANRDADNWLKTIMSPMESQVREHQVQLRRRLE
                     SIKRIHQASDTLEERVQELEQIRDGIREQEKNLENRVEAVLQVINFDKTEGKMTAEKS
                     A"
     gene            complement(1547..>3119)
                     /gene="ggt"
                     /locus_tag="AC16FV_04290"
     CDS             complement(1547..>3119)
                     /gene="ggt"
                     /locus_tag="AC16FV_04290"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR2849318.1"
                     /translation="AHPLATEAGHEILARGGNAFDAAIAVAAALAVVEPWNSGLGGGG
                     FFLLHRAHDGFEVMIDARERTPLAATRDMYLRNGRPVAALSIDGALASGIPGTPAALV
                     HLAKKYGKLPLKQTLAPAIRLARDGFPVTPLYSKMLLRQHMRLTDHFPEAARVYLKEG
                     LIPPPGDIAKQPELAQTLIRLSEKGHAGFYAGVTAERLVQGVRAAGGIWSLKDLAEYR
                     VVEREPVHGTYRGIRVTSAALPSSGGIVLNQMLNLLQGFDLNAMPKARRDHAIIEAMR
                     LAYRDRARYLGDPDFVRVDVPRLLDSAYIAKLREEMRQPQPIPVGSPAKAVGEGANTT
                     HFSIVDREGNRVAATLSLNTPFGSGLMPRGTGVVLNNEMDDFAVKPDAPNTYGLVGGD
                     ANAIAPGKRPLSSMTPTFLESDDAVVVLGTPGGSRIITMVLLATLEFAHGRGGPKEWA
                     SLPRFHHQYLPDVVTHEARAFGDDEARVLQNLGHRLETMRGGYGNMQVVTWYKKTGRL
                     EAASDPRGEGTAVVK"
BASE COUNT          668 a          980 c          955 g          516 t
ORIGIN      
        1 cagacgctgc aacacatcac cgaggtcaaa catgtcgcgc gcgaggaagc ccagacgatg
       61 ggtttccacg tggccgagga ggaatcccag accggcatgc gccttgccga ggacggcatg
      121 gtggaaattc cgaagtggcg tcacgccatc atcaatatcc cgcaccccct gctgcaacag
      181 ggactggtca ttctcgacac gcccggcctc aacgcgctcg gcgccgagcc ggagctgacg
      241 cttaatatgt tgccctcggc gcacgcggtg ctgttcattc tcgccgccga cgccggcgta
      301 accaaatccg atatcgacgt ctggcacgat catatcggcg tgcgcaagga cagcccgaac
      361 aagggccgcc tggtggtgct gaacaaaatc gacggtctgt gggacgaact gcgcgactgg
      421 gatgaggtgc agcgcgaaat cgaccgccag atccgcgaga cggcgaaaca cctcgccatc
      481 ccggaacaaa atgtattccc ggtgtcggcg cagaaggcgc tgctcggcaa ggtcaagaag
      541 gacacgaaaa tcctcgaacg ctcgcgcatc ctagcgctgg agcaggcgct cggcggcgaa
      601 atcgttccgg ccaagcgcga gatcgtgcgc gacaacatca gcggcgacat ggaggatgtc
      661 atcaaggcca tgcgcctgat catttcgcag cgcacgaaaa acgtgtacga gcacatggac
      721 gaactcggcg gcctgtccgg caagaacatg gatgtgatcg agcacatgat gaagaaggtc
      781 aaggcggaca aggaagtgtt cgagaagagc ctgcaacgct ttcaggccac gcgcagcatt
      841 ttctcgcagc agaccaacgt gctgtacacg catctcaacc tgaaaaacct cgactcccag
      901 atcgcccaga ccaagaagga aatggaaacc agcctgacca cggccgggct caaatcgtgc
      961 atggagggtt tcttcaagca cgcccaggac accatggaag aggcttccaa gcaggcccag
     1021 gaaatcaagg ccatgatgga aggcgtatac aagaaattcc aggaggaaca cggcctcggc
     1081 aacatcaagc ccggcggctt ctcggtcacg cgctacctgc gcgagatcaa gcggctccag
     1141 gagcggcacg accagtacat gaagggcatg tcgctcgtgt ttaccgagca gcgcgcgctc
     1201 acgcgcaagt tcttcgagtc ctcggtggcc aaggtgcgcg cgatctacaa gatggccaac
     1261 cgcgatgccg acaactggct caagaccatc atgtcgccga tggaatccca agtgcgcgag
     1321 caccaagtcc agttgcgccg gcggctcgaa tccatcaagc gcatccatca ggcgagcgat
     1381 accctggagg agcgcgtgca ggaactggag cagatccgcg acggcatccg cgaacaggaa
     1441 aagaacctgg aaaacagggt cgaggcggtg ctacaggtga ttaacttcga caaaaccgag
     1501 gggaaaatga ccgccgaaaa atcggcgtga ccccgcgcag ttcctcctac ttcacgaccg
     1561 ccgtaccctc gccgcgcggg tccgaagccg cttccagcct gccggttttc ttgtaccacg
     1621 tcactacctg catgttgccg tagccgccgc gcatggtttc gagcctgtgt cccaggtttt
     1681 gcaatacgcg cgcttcgtca tcgccgaagg cgcgcgcttc gtgcgtcacc acatcgggaa
     1741 gatactggtg atggaaacgc ggcaagctcg cccactcttt cggcccgccg cggccatgcg
     1801 cgaattccag tgtggcgagc aataccatgg tgatgatgcg gctgccaccc ggcgtgccca
     1861 acacgaccac ggcgtcgtcg ctttcgagaa atgtcggcgt catgctcgac agtggtcgct
     1921 tgcctggcgc aatcgcgttg gcatcgccgc ccaccaggcc gtaggtattg ggggcgtcgg
     1981 gcttgacggc gaaatcatcc atctcgttgt tcagcactac tccggtaccg cgcggcatga
     2041 gaccggaacc gaacggcgtg ttcaggctca gcgtggcggc cacgcggttg ccttcgcgat
     2101 ccacgatgga aaaatgcgtc gtgttcgcgc cctcgcctac ggccttggca ggagaaccga
     2161 cgggaatcgg ctgcggctgg cgcatttctt cccgaagttt cgcgatatag gcggaatcga
     2221 gcagacgcgg aacatccacg cgcacaaaat ccggatcgcc gagataacgc gcgcggtcac
     2281 gataagccag gcgcatggcc tcgataatgg cgtgatcgcg tcgcgccttc ggcatggcat
     2341 tcagatcgaa gccctgaagc agattgagca tctggttcaa cacaatgccg cccgaggatg
     2401 gcagcgcggc cgaggtgacg cggatgccgc ggtaggttcc atgcaccggc tcgcgttcca
     2461 ccacacggta ttcggccaga tccttcaggc tccaaatgcc gcctgccgca cgcacgccct
     2521 gcaccaggcg ttcggccgtt acgcccgcgt aaaacccggc gtgtcccttt tccgacaggc
     2581 gaatgagcgt ttgcgccagc tccggttgct tcgcgatatc gcccgggggc gggatcagtc
     2641 cttccttcag ataaacccgc gccgcttccg gaaaatgatc ggtcaggcgc atgtgctggc
     2701 gcagcagcat ttttgaataa agcggcgtca ccgggaatcc gtcacgcgcg agccggatgg
     2761 cgggcgccag tgtctgcttc agcgggagtt ttccgtattt cttggcgaga tgcaccaacg
     2821 ccgccggcgt gccgggaatg ccggaggcca gcgcgccatc gatggaaagt gctgccaccg
     2881 gtcggccgtt gcgcagatac atgtcgcgcg tggcggccag cggcgtgcgc tcgcgcgcgt
     2941 cgatcatcac ctcgaaaccg tcgtgcgcgc gatgcaacag gaaaaatcca ccgccgccca
     3001 gaccggagtt ccacggctcg accacggcca atgcggccgc gacggcgatc gcggcatcga
     3061 aggcattgcc gccgcgtgcg aggatttcat gcccggcctc ggtcgccagc ggatgggcg
//
LOCUS       DBNGZE010000167         7944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_441033, whole genome shotgun sequence.
ACCESSION   DBNGZE010000167 DBNGZE010000000
VERSION     DBNGZE010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_441033"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16FV_04295"
     CDS             <1..510
                     /locus_tag="AC16FV_04295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR2849319.1"
                     /translation="EDVRVYRNDQITVTEIEKLKPDHIVISPGPCTPNEAGVSVETIQ
                     KLGGKFPILGVCLGHQSIGQAYGGKIVRAKQLMHGKTSMIQHANKGVFAGLPNPFEAT
                     RYHSLVIERASLPDVLEITAWTDDGEIMGVRHKSLPVEGVQFHPESILTQHGHELLAN
                     FLKSHRHAA"
     gene            521..1762
                     /locus_tag="AC16FV_04300"
     CDS             521..1762
                     /locus_tag="AC16FV_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR2849320.1"
                     /translation="MHEPIHHLHHSHRHLGLRLSFTVALLAMLAPFSIDTYLPSFPDI
                     AQEFAASPVALQQTLSLYLLAFAAMMLVYGPLSDAFGRKTVVLVSAVIYVATSVGCAL
                     ATNIHWLLLMRIGQGLSASGALVVGRAIIRDAFAGPAAQRVMSQVMLIFALAPAIAPI
                     IGGWLHDAFGWRSVFWFLVLLGLVVWLWSAFFLPETLPPVGRQSGHPRAIAAAYWRAL
                     ATGRFMVLIAAIALNFGGFFLYIAGSPDVMYRHLHYGADDFGRLFVPLVAGLMLGAFI
                     SGRMAGRFTHAQAVGAGFGLMLTASGVNLGLAAWLAHTAFTIIVPVMLYATGMSLAMP
                     NLSLMALDVFPSHRGLASALQGFSQAGFNAVVAGLFAPLLSHRVEFLAAGMLALNLAG
                     LGLWFYWRYRFNAHEPATALS"
     gene            1823..2848
                     /gene="trpD"
                     /locus_tag="AC16FV_04305"
     CDS             1823..2848
                     /gene="trpD"
                     /locus_tag="AC16FV_04305"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR2849321.1"
                     /translation="MDMPSAIRAVIERRNLSGAEMGEVMRLIMTGQATPAQIGGFLIG
                     LRMKGETVEEIAAAAGVMRELATHVGVSGPHLVDTCGTGGDGASTFNISTASAFVVAA
                     AGGKVAKHGNRSVSSKSGSADVLEAAGVSLELTPEQVARCVNEVGVGFMFAPRHHGAM
                     KHAIGPRREMGVRTIFNLLGPLTNPANAPNQVLGVFSDQWVEPLAEVLQRLGSEHVLV
                     VYAEDGMDEISIGAPTHVAELKEGRVRLFTVQPEQFGLKRSEVSKLAVTSPAESLKMV
                     ESVLNNEPGPARDIVALNAGAAIYVAGLAPDIGAGIKTAQTVIASGAAKKKLHDLVAL
                     SRELAAA"
     gene            2845..3672
                     /gene="trpC"
                     /locus_tag="AC16FV_04310"
     CDS             2845..3672
                     /gene="trpC"
                     /locus_tag="AC16FV_04310"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR2849322.1"
                     /translation="MNAPPDILRKILARKAEEVAERARRAPLEELKKRLADAPAPRGF
                     QKAIRSRIATGLPAVIAEIKKASPSKGLLRTDFRPAEIAMSYERHGATCLSVLTDVDF
                     FQGSDEHLKQARAACALPVLRKDFTMDAYQVYEARVLGADAILLIVAALGDASIRELA
                     ALAEELGLDVLVEVHDEAELERALKLSTPLIGINNRDLRTFRTTLTTTLDLLDKIPHD
                     RIVVTESGIHAPCDVAQMRGRGVNAFLVGEAFMKADDPGLKLQELFDVSVQRQARKS"
     gene            3669..4088
                     /locus_tag="AC16FV_04315"
     CDS             3669..4088
                     /locus_tag="AC16FV_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR2849323.1"
                     /translation="MKANIKWTGGVSFAGTADSGHTVVMDGAPESGGQNKGSRPMELV
                     LIGMGGCTAFDVVHILRKSRQEITDCVARIEAKRAETDPKVFTNIHIHFVVTGKNLDA
                     KKVQHAIELSATKYCSASIMIGKTAKITHDFEIVESV"
     gene            4113..4511
                     /locus_tag="AC16FV_04320"
     CDS             4113..4511
                     /locus_tag="AC16FV_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2849324.1"
                     /translation="MMRPPATAGLRHVALNVKDLETCEQFYTELLGMRVEWRPDADNV
                     YLTGGNDNLALHRAASAIDPKAPQRLDHIGFIVNEIGDVDIWFEFLRSKGVKILKEPK
                     THRDGARSFYCQDPAGNTVQMIYHPPLAEK"
     gene            complement(4516..5160)
                     /gene="coq7"
                     /locus_tag="AC16FV_04325"
     CDS             complement(4516..5160)
                     /gene="coq7"
                     /locus_tag="AC16FV_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR2849325.1"
                     /translation="MTDRTLTALDRLMGQLDQALHTVFGPAPTPGRASPATGKADTGL
                     STSERELSGRLMRINHAGEICAQALYQGQAATARLPEVRGKMEQASQEENDHLAWTEE
                     RIRELGGHTSYLNPLWYAGSFAIGALAGVIGDKWSLGFVAETEKQVVKHLDGHLQRIP
                     TEDSKSRAILEQMRDDEGRHATVAIESGGAELPEPVRQIMRCTSKIMTRTAYWI"
     gene            complement(5194..6441)
                     /locus_tag="AC16FV_04330"
     CDS             complement(5194..6441)
                     /locus_tag="AC16FV_04330"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR2849326.1"
                     /translation="MNPDFHIKREAAKCVACGLCLPHCPTYNLLHDEAESPRGRLSLM
                     LALAKDDLPLSAKLESHLARCLDCRACEKVCPSYVAYGQALDSARTLIESKRSTTAKP
                     VNRSVTLIQWLAEKPARMRALGKILRLYQWTGLQWLLRKSHALRLVGLNGLDAAIPKL
                     QAQKVFAEIYPTKNKSKGRIALFTGCQASLTDQQALAASIRLLNHLEYEVDVQPAQGC
                     CGALHLHSGQAAKAAGLMRQNIAAFVNGGDEIVSVASGCAATLCEYGKYLDDEAAQKF
                     SVRIRDINQFLADAVWPEDIAFRPLPKRIAVHDPCTLINVLRQENKPYALLAKIPGAE
                     IIPLPENKVCCGAAGTYYLTQTSIAEQLRTPKIDHLRRLAPDMLVTSNPGCATFLAAG
                     LREAGLAIEVLHPVTLLERQLKT"
     gene            6555..6869
                     /locus_tag="AC16FV_04335"
     CDS             6555..6869
                     /locus_tag="AC16FV_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR2849327.1"
                     /translation="MFYYRYHVFFCVNKREDGSACCANHPAQQMRDYAKARIKSLGMD
                     GKGGVRINQAGCMDRCEEGPVIAVYPEGTWYTYVDQVDIDEIIDRHLRQGQPVERLKL
                     KE"
     gene            7462..7890
                     /gene="rplM"
                     /locus_tag="AC16FV_04340"
     CDS             7462..7890
                     /gene="rplM"
                     /locus_tag="AC16FV_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR2849328.1"
                     /translation="MKTFSAKQESVKRDWYVIDATGKVLGRVATEIARRLRGKHKAEY
                     TPHVDTGDYIIVINADKLQVTGKKSRDKIYHRYSGYQSGLKAASFQDMMKKAPGDVIE
                     TAVRGMLPKNPLGRQMFRKLKVYRGANHRHTAQNPKVLEI"
BASE COUNT         1567 a         2233 c         2437 g         1707 t
ORIGIN      
        1 gaggacgtgc gggtgtaccg caacgaccag atcacggtta cggagattga aaaactcaaa
       61 ccggatcaca tcgtcatttc accgggaccg tgcacgccga atgaggcggg cgtgtcggtg
      121 gaaacgatac agaaactggg tggcaagttt ccaattctgg gcgtatgcct cggacaccag
      181 agcatcggcc aggcctatgg cgggaaaatc gtgcgcgcga aacagttgat gcatggtaag
      241 acctccatga tccagcacgc gaacaaaggt gtgttcgccg gtctacccaa tccgttcgag
      301 gcgacgcgtt accattcgct tgtcattgaa cgcgcgagcc tgccggatgt cctcgagatc
      361 accgcctgga ccgacgacgg cgagatcatg ggcgtgcggc acaagtccct gccggtcgaa
      421 ggtgtgcagt tccatccgga atccatcctc acgcagcacg gtcacgaact gctggcgaat
      481 tttctgaaga gccatcggca tgcggcctga gccggtcgcg atgcacgaac cgatacatca
      541 tttacatcat tcccatcgcc atctcggcct gcggctctcg ttcaccgtgg cgctgctcgc
      601 catgctggcg ccgttcagca tcgataccta tttgccgtcg tttccggata ttgcgcagga
      661 gtttgcggca tcgcccgtcg ccctgcagca gacactgagc ctgtatttgc tggcgtttgc
      721 cgcgatgatg ctggtgtacg gcccgttgtc ggacgccttc gggcgcaaaa ccgtggtgct
      781 ggtatcggca gtgatatatg tggcgacctc cgtcggttgt gcactggcca ccaatattca
      841 ttggctgttg ttgatgcgca tcggtcaggg attgtccgcc agtggcgcac tcgtggtcgg
      901 ccgcgcgatc atccgcgacg cctttgccgg gcccgcggcg cagcgcgtca tgtcgcaggt
      961 catgctgatc ttcgcgctcg cgcctgcgat cgctcccatc atcggcggct ggctgcacga
     1021 cgccttcggc tggcgttcgg tgttctggtt cctggtgctg ctggggctgg tcgtctggct
     1081 gtggtcggca ttctttttgc ccgagacctt gccgccggtg ggaagacagt cgggacatcc
     1141 gcgcgccatc gccgcggcct attggcgggc gctggccacg ggccgcttca tggtattgat
     1201 cgccgccatt gcactcaatt tcggcggatt ttttctctac atcgctggtt cgccggatgt
     1261 gatgtaccgc cacctgcatt acggcgcgga tgacttcggg cgcctgttcg tgccgctcgt
     1321 ggccgggttg atgctggggg cctttatttc cggcaggatg gccggacgct ttacgcacgc
     1381 tcaggccgtc ggcgcgggtt ttgggctgat gctgaccgcc tccggcgtga atctggggct
     1441 ggcggcgtgg ctggcgcaca cggcgttcac gattatcgtg ccggtgatgc tgtatgccac
     1501 cggcatgtcg ctggccatgc ccaacctgag tctgatggcg ctcgatgtgt ttccctcgca
     1561 ccggggtctg gcgtcggcgc tccagggatt cagccaggcg ggtttcaacg ccgtggtggc
     1621 aggcctgttt gcgccgctac tttcacaccg tgtggaattc ctggcggccg gcatgctggc
     1681 gctcaatctg gcgggtttgg gactgtggtt ttactggcgg tatcggttca acgcccatga
     1741 gccggcaacc gctttgtctt gaccgtcgtt gcggttcggc gacagaatcg cccccttgtc
     1801 attttcatcg ttgagataat tcatggacat gccatccgcc atccgcgccg tgattgaacg
     1861 gcgcaacctc tcgggcgccg agatgggcga agtcatgcgc ctgatcatga ccggacaggc
     1921 cacgccggcg cagatcggcg gttttctcat cggtctgcgc atgaagggcg agactgtcga
     1981 ggagatcgcg gccgccgccg gcgtcatgcg cgaactcgcc acgcacgtgg gtgtcagtgg
     2041 accgcatctg gtggatacct gcggcaccgg cggcgacggc gccagtacct ttaatatctc
     2101 caccgcctcc gccttcgtgg tggccgccgc cggcggcaag gtcgccaagc acggcaaccg
     2161 ctcggtatcg agcaagtcgg gcagcgccga cgtgctcgag gccgcgggcg tgagcctgga
     2221 actgacaccg gagcaggtgg cgcgctgtgt caatgaagtg ggcgtgggct tcatgttcgc
     2281 ccccaggcat cacggcgcga tgaagcatgc catcgggccg cggcgtgaga tgggcgtgcg
     2341 cacgattttt aacctgctcg ggccgttgac caacccggcg aacgcgccca atcaggtgct
     2401 cggcgtgttt tccgatcaat gggtcgaacc gctggcggag gtgttgcaga gacttgggag
     2461 cgagcacgta ctcgtggtgt atgccgagga cggcatggac gagatcagta ttggcgcgcc
     2521 tacgcatgtg gcggaactga aagaggggcg tgtgcggctc ttcaccgttc agccagaaca
     2581 gtttgggctg aaacgcagcg aggtgtcgaa gcttgccgtc accagcccgg ccgagagcct
     2641 gaagatggtt gaatcggtgc tgaacaacga acccggaccg gcgcgcgaca tcgtggcgct
     2701 caatgccggg gcggcgatct atgttgcagg cttggccccc gatatcggcg ccggtatcaa
     2761 gaccgcacag accgtgatcg ccagcggcgc agccaaaaag aaattgcatg atctggttgc
     2821 gttgagccgg gagctggctg ctgcatgaac gccccaccgg acatacttcg caagattctg
     2881 gcgcgcaaag ccgaagaggt cgccgagcgt gcgcgccgtg cgccgctgga ggagctgaaa
     2941 aagcgccttg cggatgcgcc ggccccgcgt ggttttcaga aggcgatccg cagccgcatt
     3001 gcgaccggac tgcctgcggt gatcgccgaa atcaagaagg cgagcccgag caagggcctg
     3061 ttgcggacgg atttccggcc ggccgagatt gccatgagtt acgagcgcca tggcgctacc
     3121 tgcctgtcgg tgctgacgga cgtggatttt ttccagggca gcgatgagca cctgaaacag
     3181 gctcgcgccg cctgcgcgct gccggtgttg cgcaaggatt tcaccatgga tgcgtaccag
     3241 gtttacgagg ctcgtgtttt gggtgccgac gccattctgc ttattgtggc tgcgctgggc
     3301 gatgcgtcga tacgcgaact ggcggcactg gccgaggagc tcggcctgga cgtgctggtg
     3361 gaggtgcacg atgaagcgga gctcgagcgc gcgctgaagc tctccacgcc gctgattggt
     3421 atcaacaacc gcgacctgcg tacgttccgc acgactttga cgaccacgct cgacctgctg
     3481 gacaaaatcc cgcatgaccg catcgtggtt acggagagcg gcattcatgc gccctgcgac
     3541 gttgcgcaga tgcgtggccg cggggtgaat gcctttctgg tgggcgaggc cttcatgaag
     3601 gccgacgatc cgggcctgaa acttcaggaa ttgttcgatg tatcggtgca acgacaagcg
     3661 agaaaatcat gaaggcgaat atcaagtgga ccggcggtgt cagctttgcc ggcacagcag
     3721 acagcggcca cacggtggtc atggacggcg cgccggaatc cggtggacag aacaagggct
     3781 cacggcccat ggagctggtg ctgatcggca tgggcggatg cacggcattc gatgtcgtgc
     3841 atattctgcg caagtcgcgg caggagatca cggattgtgt cgcccggatc gaggccaagc
     3901 gggccgagac cgatcccaag gtattcacga acattcacat ccattttgtc gttactggaa
     3961 agaacctgga cgccaaaaaa gtgcagcacg ccattgagct ctcagcgacc aagtattgct
     4021 ccgcctcgat catgatcggc aagaccgcca agatcacaca cgactttgag attgtcgaaa
     4081 gcgtttaaac gacggaaatc cagtaaaaat ttatgatgcg tccacccgct accgccggat
     4141 tgcgccacgt ggcgctgaac gtgaaggacc tcgagacctg tgagcagttt tacacggaac
     4201 tgctgggcat gcgcgtggag tggcggccgg atgccgataa cgtttatctg actggcggca
     4261 acgacaatct ggctctgcac cgtgccgcgt ccgccatcga cccgaaagcg ccgcagcgac
     4321 tcgatcacat cggctttatc gtcaatgaaa tcggagatgt ggatatctgg tttgaatttc
     4381 tcaggtcaaa gggtgtgaaa atactgaaag aacccaagac ccatcgcgat ggtgcgcgca
     4441 gtttctattg tcaggacccg gcgggtaaca cagtgcagat gatttaccat ccgccgctgg
     4501 cggagaagta atcttttaaa tccaatacgc cgtacgcgtc atgattttcg acgtgcagcg
     4561 catgatctgc cgcacgggtt ccggcagctc ggcgccacca gactcgatgg ccacggtggc
     4621 atgacggcct tcgtcgtcgc gcatctgttc caggatcgcc cggctcttgc tgtcttccgt
     4681 cgggatccgc tgcagatgcc cgtcaagatg tttcaccacc tgcttctcgg tttcggcgac
     4741 aaagccgagg ctccatttgt cgccgataac gcccgccagc gcgccgatgg cgaacgagcc
     4801 cgcgtaccag agcggattca gatagctcgt atgaccgcca agctcgcgga tgcgctcctc
     4861 ggtccaggcc agatgatcgt tttcttcctg tgatgcctgt tccatttttc cgcgcacctc
     4921 cggcagccgc gccgtggctg cttgcccctg gtacagggcc tgtgcacaga tttcgccggc
     4981 atgattgatg cgcatcagcc ggcccgacaa ttcacgctcc gatgttgaca gcccggtgtc
     5041 agccttgccg gtcgcgggac tggcgcgccc gggagtgggc gccggcccga acacggtatg
     5101 caacgcctga tcgagctgtc ccatcaggcg gtcgagggcg gtaagagtgc ggtcggtcat
     5161 aacgtatgag tatcaaactt aaggcctgaa gcttcaagtc ttaagctgtc tttcgagcag
     5221 ggttaccgga tgtaagacct cgatggccag cccggcctcg cgcaaaccag ccgccagaaa
     5281 cgtggcgcaa ccggggttcg aggtcaccag catgtccgga gcgagacgcc ggagatgatc
     5341 gattttcggc gtgcgcaatt gttcggctat tgatgtctga gtcagatagt aagtaccggc
     5401 agcaccgcaa cagaccttgt tttccggcag cgggatgatt tccgcgccgg gaattttcgc
     5461 cagcagggca tatggcttgt tctcctgacg caaaacattg atcagcgtgc atggatcatg
     5521 tacggcaatt cgcttgggca acgggcggaa tgcgatatcc tccggccata ccgcgtcagc
     5581 caggaattga ttgatgtccc tgatgcggac ggaaaatttt tgcgcagcct catcgtccag
     5641 atattttcca tattcgcata gcgtggcggc gcaaccgctg gccacggata cgatctcgtc
     5701 acccccattt acgaatgcag cgatgttctg tcgcataagt ccggcggctt ttgcggcttg
     5761 accgctatgc agatggaggg caccgcaaca accctgtgct ggctgcacgt ctacttcata
     5821 ttccaagtgg ttgagcaacc ggatcgaggc cgcaagtgcc tgctggtccg tcaggctcgc
     5881 ctgacagccg gtgaagaggg cgatccggcc cttcgactta ttttttgtgg gatagatttc
     5941 agcgaaaact ttctgggctt gaagcttcgg aatagcggca tcgagcccgt tcagcccgac
     6001 aagtctgagc gcatggcttt tccgcagcag ccattgcaat ccagtccatt ggtacaaacg
     6061 cagaattttt cccagcgcac gcattctggc gggtttttcc gccagccatt gaatcagcgt
     6121 cacggatctg ttcacgggtt tggcggtcgt ggaacgcttg gattctatta gcgttcgtgc
     6181 ggagtcgagt gcctgtccat aggctacata ggaaggacag actttttcgc aagcacggca
     6241 gtccaggcaa cgcgcaagat gggattcgag tttggcgctc agcggcaggt catcttttgc
     6301 cagcgccagc atcagcgaca ggcgcccacg cggcgactcg gcttcgtcgt gcaacaggtt
     6361 ataggtcgga caatggggca ggcacagtcc gcaggccacg catttggcgg cctcgcgttt
     6421 gatatgaaag tcggggttca agtcaggcgg tcaagaacag tgtggcaagg gataaatatg
     6481 cccaagaggg gccgatgacc tataattccg ttacgttatc gtctgttgcc cattgcttgc
     6541 aactttccaa acccatgttc tattaccgtt accatgtttt tttctgcgtg aacaaacgcg
     6601 aggacggcag cgcctgttgc gccaaccacc cggcgcaaca gatgcgcgac tatgccaagg
     6661 cgcgtatcaa gtcgctgggc atggacggca agggtggcgt ccgcatcaat caggcgggat
     6721 gcatggatcg ttgcgaagag ggaccagtca tcgcggttta ccctgagggc acctggtaca
     6781 cctatgtgga ccaggtagac atcgacgaga tcattgaccg gcacctgaga cagggtcagc
     6841 cggtcgagcg tctgaaattg aaggaatgac ccctgtcgct tccgctttgc ctcaagtgca
     6901 tgggggtgat ggagatcgtc ggttggcttg gagcggattt atcatctgct tttgaaccaa
     6961 ttttggacga taaggtcaca gttgctgagc gagagttatc gaaaaccaga agagggtatg
     7021 tattatttgg tatttaaatc attgagttac tgtagtactt gatgaatttt gcaggttgta
     7081 ttcaaacagt gttgacaata ggtaggaata ttagataata attatatact ttatcagcaa
     7141 gctttctggt tattggccca aagtttgcta atctgaatat atgtagttga aagatttctt
     7201 tacggcgctc aggcgccggg taatggctcc tccatcctcc tagtggtggt tcgggcgtgg
     7261 ctccccagcc acgccttttt tttgcctgtt ttccggcgca cgtgctggcc ctcttctgtt
     7321 gtcgttgaag accggattga tgatttttcc ttgtaactca tgagcgtctt ttgtaatatg
     7381 cgcggctttc cggcgggatg tctcgcaccg aaactgcgtg gcgcgaagcc ttcaagcaat
     7441 tgaattaccc gaagaatttt catgaaaaca ttttccgcaa aacaggaaag cgtcaaacgc
     7501 gactggtatg tgatcgatgc caccggcaag gtgctcgggc gcgtggcgac cgagattgcg
     7561 cggcgtctgc gcggcaaaca caaggctgaa tatactccgc acgtggacac gggtgattac
     7621 atcatcgtga tcaacgccga caaactgcaa gtgacgggca agaagagtcg cgacaagatt
     7681 tatcatcgct attccggtta ccagagcggc ctgaaagcgg cgagcttcca ggacatgatg
     7741 aaaaaggcgc cgggtgacgt catcgaaacg gcggtgcgcg gcatgctccc gaagaatccc
     7801 ctcggccgcc agatgttccg caagctcaag gtataccgcg gcgcgaatca ccggcatacg
     7861 gcacagaacc ccaaggttct ggaaatctaa atcttcaggt aacgaatacc atggcgcagg
     7921 caaacatcca tctcggcacc ggac
//
LOCUS       DBNGZE010000168         2568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1344444, whole genome shotgun sequence.
ACCESSION   DBNGZE010000168 DBNGZE010000000
VERSION     DBNGZE010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1344444"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="AC16FV_04345"
     CDS             <1..1064
                     /locus_tag="AC16FV_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR2849329.1"
                     /translation="TVTVIRLRGEEESAVGIATVIITLVILILTDLAPKTLAALHPER
                     IAFPSAFLLRPMLWLIYPVVWVVNGMANGLLRLIGVRVQAKPIDQVSPEELRAIIMEA
                     GVLIPESHQDMLLAILDLEKITVDDVMVPRGKIEGINLDAEWDELIGQITGSRYTRLP
                     VFRGSLDNVSGMIHVRRVLNLMREGKLDKESLEQAIVEPYFIPSGTTLTTQLLNFRQV
                     KRRIGLVVDEYGDIQGLVTLDEILEEIVGDFTAHAMGKIEDVHPQSDGSYLVRGTVNL
                     RDLNRQLDWKLPTDESRTLNGLIVEYLEDIPEPGTSLMIHGYQVEILRTRGTAIEIAR
                     VRPATETAPETSSTPSSAS"
     gene            1078..2025
                     /locus_tag="AC16FV_04350"
     CDS             1078..2025
                     /locus_tag="AC16FV_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR2849330.1"
                     /translation="MERIVFLDRDSIQAEIRRPGFAHEWRDYPATVAAQVSERLNDTT
                     IAISNKVPLMAASLTPLKNLKMIAVCATGTNNVDLDYCRVHGIVVSNIRNYAMHTVPE
                     HVFAMVLALRRNLLGYREDVNNGLWQKAEQFCLFTRPILDLHGGTLGVVGHGALGQAT
                     AKLGEAFSMRVLIAEHKGTAQTRPGYTPFETVLTESDVVTLHCPLTPATHHLIGAPEF
                     RKMRRHALLINTARGGLVDETALVQALKEGVIGGAGFDVLTTEPPRAGNPLLDLRLPN
                     FILTPHVAWASRNAMQIMADQLIDNIEAFVRGALQNRVV"
     gene            2055..>2568
                     /locus_tag="AC16FV_04355"
     CDS             2055..>2568
                     /locus_tag="AC16FV_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HIR2849331.1"
                     /translation="MTPAKTQRTVFFVSDRTGITAELLGKTLLTQFPGIEFQKRTFPF
                     VDTVEKARTTLAKIDETARREGHKPIVFSTLINEDVRTALASDNALVIDLFADFIRRL
                     EAELGLESSHALGLSHGMGEELAYQKRMDALNYTLSHDDGMHTNNYDRAEVILVGVSR
                     TGKTPTCLYLA"
BASE COUNT          541 a          807 c          741 g          479 t
ORIGIN      
        1 caacggttac cgtcatccgc ctgaggggcg aggaagaaag cgcggtcggc atcgccaccg
       61 tcatcatcac gctggtgatc ctgattctca ccgacctagc gccgaaaacg ctcgccgcgc
      121 tgcatccgga gcgcatcgcc ttccccagcg ccttcctgtt gcggccgatg ctgtggctca
      181 tctacccggt ggtgtgggtg gtcaacggca tggccaacgg actgttgcgg ctcataggag
      241 tacgggtcca ggccaaaccg atcgaccagg tgagtccgga agagctgcgc gccattatca
      301 tggaagccgg ggtgctgata ccggaaagcc atcaggacat gctgctggcg attctcgatc
      361 tggaaaaaat caccgttgac gacgtgatgg tgccgcgtgg aaaaatcgag ggcatcaatc
      421 tcgatgccga gtgggacgag ctcatcggcc agatcaccgg cagccgctat acccgcctgc
      481 cggtttttcg cggcagcctc gataacgtca gcggcatgat ccatgtgcgc cgggtgctga
      541 acctgatgcg cgagggcaag ctcgacaagg aatcgctgga acaggccatt gtcgagccct
      601 atttcattcc ctcgggcacg acgctcacga cgcagttgct caacttccgg caagtgaagc
      661 gccgtatcgg cctcgtggtc gacgaatacg gcgacattca ggggctggtc acgctggatg
      721 aaatcctcga ggaaatcgtc ggcgatttca ccgctcacgc catgggcaaa atcgaggacg
      781 ttcaccccca gagcgacggc agttacctcg tccgcggcac cgtcaacctg cgcgacctga
      841 accgccagct cgactggaaa ctgcccaccg acgaatcgcg caccctgaac ggcctgatcg
      901 tggaatacct ggaagacatc cccgagcccg gcacgagcct catgatccac ggctaccagg
      961 tggaaatcct gcgcacccgc ggcaccgcca tcgaaatcgc gcgcgtgcgg ccggcaaccg
     1021 aaaccgcgcc tgagacatcc tctaccccat cctctgcctc ctgaaccgga tttgcctgtg
     1081 gaacgcatcg tctttctcga tcgcgacagc atccaggctg agatacgccg acctggtttt
     1141 gcgcatgagt ggcgggatta ccccgccacc gtggccgcgc aggtcagcga gcgcttgaac
     1201 gacacgacga tcgccatcag caacaaggtt ccactcatgg ctgccagcct cacgccgctc
     1261 aaaaacctca agatgatcgc ggtgtgcgcc acaggcacca ataacgtcga tctcgattat
     1321 tgccgcgtgc atggcatcgt cgtttccaat atccggaatt acgcgatgca caccgtcccg
     1381 gaacatgttt tcgccatggt actggcgttg cggcgcaatc ttttgggtta tcgcgaagat
     1441 gtaaacaacg gactatggca aaaggccgag cagttttgcc tgttcacgcg ccccattctg
     1501 gatttgcatg gcggcacgct gggcgtggtc ggccatggcg ctctgggcca agccacggca
     1561 aaactgggcg aagcattcag catgcgcgta ttgatcgctg aacacaaagg cacggcgcaa
     1621 acgcgacccg gttatactcc cttcgaaacg gttctgacgg agagtgacgt ggttaccctg
     1681 cactgcccgc tcacgcccgc gacgcaccac ctgatcggcg cgccggaatt caggaaaatg
     1741 cgccgtcacg ccctgctcat caacaccgct cgcggtggtc tggtggatga aacagcgctc
     1801 gttcaggcgc tcaaggaggg agtgatcggt ggggccggat tcgatgtgct gaccacggaa
     1861 ccgccgcgcg cgggcaaccc gttgctggat ctgcggctgc ccaacttcat cctgacaccg
     1921 catgtcgcct gggccagccg caacgccatg caaatcatgg cggaccagct gatcgacaat
     1981 atcgaggcct ttgtccgcgg cgcactgcag aatcgggtcg tctgaccgct attacaggat
     2041 tacaatcggt gcacatgacg cccgccaaaa cccagcgcac cgtatttttc gtttccgatc
     2101 gcaccggcat caccgccgag ctgctgggca agacgctgct gacgcagttt cccggtattg
     2161 agttccagaa acgtacattc cctttcgtgg atacggtgga aaaggccaga accacgctgg
     2221 ccaagataga tgaaaccgcc cgccgggaag ggcacaaacc catcgtcttc agcacgctga
     2281 taaatgaaga cgtgcgcacg gcgttggctt ccgataatgc tttggtcatc gatctcttcg
     2341 cggatttcat ccggcgtctg gaggcggaat tgggcttgga gtcgtcccac gccctcgggc
     2401 tcagtcatgg catgggcgaa gagctggcct accagaaacg catggatgcg ctgaactaca
     2461 cgctgtccca tgatgacggc atgcatacca acaactacga ccgggccgag gtgatcctgg
     2521 ttggcgtgtc gcgcaccggc aagacgccca cctgccttta tctggcga
//
LOCUS       DBNGZE010000169         5684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1483561, whole genome shotgun sequence.
ACCESSION   DBNGZE010000169 DBNGZE010000000
VERSION     DBNGZE010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1483561"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16FV_04360"
     CDS             <1..198
                     /locus_tag="AC16FV_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR2849332.1"
                     /translation="IADALRLVLEKDPLIHADQIGVRVKNRIVTLTGLVGNKEEKKMA
                     ELDAWYLLGVNKVVNGIDIRH"
     gene            250..810
                     /locus_tag="AC16FV_04365"
     CDS             250..810
                     /locus_tag="AC16FV_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011770966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR2849333.1"
                     /translation="MKLPLQVVFRNMEPSAAIEAAVRERADKLDLFYEHIMSCRVVIE
                     LHHRHHHQGNLYHVRVDVKVPEAEIVASREPGEHHAHEDVYVAIRDAFNAVRRRLEDY
                     IRRRRGLVKAHEAPPHGRVVELYPDYGRIETLDGRLVYFHKNSVAGGDFDHLDVGTEV
                     RFVEEQGELGPQASTVHVIGKHHLVS"
     gene            886..1533
                     /locus_tag="AC16FV_04370"
     CDS             886..1533
                     /locus_tag="AC16FV_04370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008380247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR2849334.1"
                     /translation="MKVSSHPMTAAMTTTRSMEGISLPVPRQDGDFSIERALHERRSV
                     REFGKSALTQEEVSQLLWAAQGITSRDGLRTAPSAGALHPLELYLVVGMVRDLDPGVY
                     KYAPAGHKLVKIIAGDQRHKLASAAHGQDCVADAAAVLVFTAVERRTTRKYGSRGVRY
                     VHIEIGHASQNVFLQAMALGLGTVVVGAFEDDTVGELLQLPEGEAPLYLMPLGRP"
     gene            1572..2108
                     /locus_tag="AC16FV_04375"
     CDS             1572..2108
                     /locus_tag="AC16FV_04375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR2849335.1"
                     /translation="MKVLIISANRFEDSELLVPYYRLREEGIAVDIASLTKGFIKGKH
                     DYEIEANIAIADVHPEQYSALVLPGGKAPAALRENKRVLDVVRAFFEAGKPVSAICHG
                     PQILISAGVLKGRTATCYRSVAEELRGAGVNYHDAEVVVDGNLVTSRQPSDLPAFLRE
                     TMKKIHALGEHAPRVAAG"
     gene            complement(2131..2952)
                     /locus_tag="AC16FV_04380"
     CDS             complement(2131..2952)
                     /locus_tag="AC16FV_04380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR2849336.1"
                     /translation="MQDSDCVEFLQWALPRLHRRWPGFRKVRAQICKRIDRRYKVLGL
                     PGPRAYRDYLEQHPAEWEPLDRLCPITISSFYRDKAVFDFLGATLLPQLARDACQHAT
                     RDLRALSIGCASGEEPYTLKLAWHFRVAPVHREITLPIVATDVLPEMLERAREACYPS
                     SSLKRLPEAWRALAFEPVAGGYCLRAEYRQGIEFLQQDVRTTLPDGIFYLILCRNLVF
                     TYYDEGTQRQILARLLTRLAPGGALVIGRRETLPSGTSGLGPWPGTERLGIYRHG"
     gene            complement(2992..3261)
                     /locus_tag="AC16FV_04385"
     CDS             complement(2992..3261)
                     /locus_tag="AC16FV_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849337.1"
                     /translation="MDLTAKNLAEAKETVGILLEQLGLTAYLFEVEPRAERWEVRVEC
                     ALDSGWQSSVFTVEDTALRACRTDRLVRDQILGELHKRLAPPDTG"
     gene            complement(3272..3811)
                     /locus_tag="AC16FV_04390"
     CDS             complement(3272..3811)
                     /locus_tag="AC16FV_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HIR2849338.1"
                     /translation="MLDWNVVVSIREDCYKLAHKLLQAFGRVDHTDYYNVFALKVEDV
                     QGFLDALRQTTEQDSALARCLARVIPATRSFAFQSAEEFEARVKEAAAGFVPDLLGKT
                     FYVRMHRRGFHQKLASQREEQLLDVFLLEQLARTEAPGKIRFADPDAVIAVETIGPWA
                     GLSLWKREDRARYPFVRVN"
     gene            complement(3821..4327)
                     /locus_tag="AC16FV_04395"
     CDS             complement(3821..4327)
                     /locus_tag="AC16FV_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HIR2849339.1"
                     /translation="MGTAARKVSTSPRRIRRDRYIKEFEHDTYKTKGKLREPTVCSEC
                     GAVFHKGRWQWLKKPKGAHETLCPACHRTHDKYPGGYLTLSGPFQKQHGDEILRLAKN
                     NEAREKKEHPLRRIMSIDKQGDDILITTTTMGMARVIGDAVHDAYKGELCYQYTDEAN
                     ILHVAWKR"
     gene            4452..4649
                     /locus_tag="AC16FV_04400"
     CDS             4452..4649
                     /locus_tag="AC16FV_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR2849340.1"
                     /translation="MNQRESQRRLAEVVRDACGKAAQEAYENAGISGLCEEGRWECAV
                     AAIRALDVEAVINRLPDDLSK"
     gene            complement(4659..>5684)
                     /locus_tag="AC16FV_04405"
     CDS             complement(4659..>5684)
                     /locus_tag="AC16FV_04405"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0033499 - galactose catabolic process via
                     UDP-galactose, Leloir pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR2849341.1"
                     /translation="ESVLVATARGMRPKKAEAPAPRDTRAHVETCPFCTGNEHMTPPV
                     IAAYPAEKDWEIRIVENLYPVLGDDRQNSNLTFGLQQTIDGYGRHEVMIDHNQHGIAI
                     HEMSEAHLALLFGAYRDRMEQLYKSNSRLRYVLAFKNFGPAAGASIAHTHSQVIATPV
                     IPDNVQTEVANSRAFYHKNHHCIFCSLIDEALTFEATIYDRESGEIRRKINVGQYVIE
                     RVGKFIAIKPFASRYEWEVHILPLAHQSDFLRISKGDLDDLARVMRRTMARLDAVLGG
                     AQYNYFLHSLPHDVQCEDCEASYHWHLEITPRTSIPTGFELGSGLFVNTVAPEMAAEK
                     LRAINLD"
BASE COUNT         1081 a         1658 c         1825 g         1120 t
ORIGIN      
        1 attgccgacg cgctgcgcct ggtgctggaa aaagaccccc tgatccatgc cgatcagatc
       61 ggtgtccgtg tgaagaacag gatcgtcacg ttgactggct tggtcggcaa taaggaagaa
      121 aagaagatgg cggaactgga tgcctggtat ctgctgggtg taaacaaggt cgtgaatggt
      181 attgatatac gccattagcc ttcggaacgt acaccgcggc atggcctgcg ccaacaagag
      241 ggtaagcaca tgaaactgcc attacaggtt gtattccgta acatggagcc gtccgcggcg
      301 atcgaggcgg ctgtccggga gcgggcggac aagctcgatc tgttctatga acacatcatg
      361 agttgtcgcg tggtgatcga gctgcatcac cggcaccatc atcagggcaa tctctaccat
      421 gtacgcgtgg atgtgaaggt gcccgaggcg gagatcgtgg cgagccgcga accgggcgag
      481 caccacgccc atgaggatgt gtatgtcgcc atacgcgatg cgttcaacgc ggtgcgacgc
      541 cgactcgaag actatatcag gcgccgccgt ggcttggtga aggcgcacga agccccgccg
      601 catgggcggg tagtggagct gtatccggac tatggcagga tcgaaacgct ggacggccgg
      661 ctcgtctact ttcacaagaa cagcgtggcc ggcggggatt tcgaccacct tgatgtcggc
      721 acggaagtgc gcttcgtcga ggagcagggc gagctcggac cgcaggccag cacggtccat
      781 gtcatcggca agcatcacct tgtcagttag gttccgttcc ttgcctgcac ggagcgggcc
      841 ggcgttgcct gcgcgggcag cgcctggcgc cccggagtac tcacgatgaa agtttcatcc
      901 catcccatga ctgccgcgat gacgacgacg cgatcgatgg agggaatttc gctgccggtg
      961 ccgcgtcagg acggcgattt ctccatcgag cgcgcgctgc atgagcgccg ttccgtccgt
     1021 gagttcggca aaagcgcgct gacgcaggag gaggtttcgc agctcctgtg ggcggcccag
     1081 ggcatcacga gccgcgacgg tttgcgcacg gcgccttccg ccggtgcgct tcatccgctg
     1141 gaactgtatt tggtcgtggg gatggtgcgc gatcttgatc ccggggtcta taaatacgcg
     1201 cccgccgggc acaagctcgt gaagatcatc gcgggcgatc agcgtcataa gctcgcttcg
     1261 gccgcccacg gccaggactg cgtggcggac gcggccgcgg tgctggtttt caccgccgtc
     1321 gagcggcgca ccacacgcaa gtacggtagc cgtggtgtgc gctatgtgca catcgagatc
     1381 ggtcacgctt cgcaaaacgt ttttttgcag gcgatggcgc tgggtctcgg cacggtggta
     1441 gtgggcgcct ttgaggatga cacggtggga gaactgctcc agttgccgga aggcgaagcg
     1501 ccgttatatc tcatgccgct gggacggcct tagagtctgg cacggaagca agatgcacac
     1561 aggaggcgtt tatgaaagtg cttatcatca gtgccaatcg attcgaggat tccgagttgc
     1621 tggttcccta ctaccggctc cgggaggaag gtatcgcagt ggatattgcg tcattgacca
     1681 aagggttcat caagggcaaa cacgattatg agatcgaggc caatattgcc attgccgatg
     1741 tgcatccgga acaatacagc gcgctggtgc tgcccggcgg caaggcaccg gcggcgctgc
     1801 gcgaaaacaa gcgcgtgctc gacgtggtcc gcgccttctt cgaggccggc aagcccgtct
     1861 ccgccatctg ccatgggccg cagattctga tttccgccgg tgtgctcaag ggccggacgg
     1921 cgacctgtta ccggtcggtg gcggaagagc ttcgcggcgc cggggtgaat taccatgacg
     1981 ccgaggtcgt ggtggacggc aatctcgtga cgtcgcgcca gccttccgac ctgcctgctt
     2041 ttctgcgcga aaccatgaaa aagattcacg cccttgggga acacgccccg cgcgtggcgg
     2101 cgggataaag cgactctggc agccggcgtc tcagccatgg cggtaaatac cgagcctctc
     2161 cgtgcccggc catggcccca accccgaggt tcctgacggg agggtttcgc ggcgtccgat
     2221 cacaagcgcg ccaccgggcg caagacgggt cagcaggcgc gccaggattt gccgctgcgt
     2281 accctcgtca taataggtga acaccagatt acggcagaga atcaaataaa aaattccatc
     2341 tggcagcgtg gtacggacat cctgttgcag gaactcaatc ccctgacggt attccgcgcg
     2401 cagacagtag ccgcccgcca cgggctcaaa ggccagggcg cgccaagctt cgggcagccg
     2461 cttgaggctg ctggagggat agcaggcctc gcgcgcgcgt tcaagcatct ccggtaaaac
     2521 gtcggtggcg acgataggca gcgttatttc gcgatgaacc ggcgcgacac gaaaatgcca
     2581 tgcgagcttt agcgtgtagg gttcctcccc ggaagcacag cctatgctca gcgcgcgcag
     2641 atcgcgcgtg gcgtgttgac acgcgtcccg cgccagttgc ggtaacagcg tggcgccaag
     2701 aaaatcgaag accgccttgt cgcgatagaa gctggagatc gtgatggggc agagccggtc
     2761 gagcggttcc cattccgccg ggtgttgttc gagatagtcg cggtaggccc gcggtccggg
     2821 gaggccaagc actttgtagc gtcggtcgat acgcttgcag atttgcgcgc gcaccttgcg
     2881 gaatccgggc cagcgtcgat ggagccgtgg cagcgcccac tgcaggaatt caacgcaatc
     2941 ggagtcttgc attgaacggt ttttccaacc ggatggggac ggtatcggcg ctcagccagt
     3001 atccggcggg gccaggcgct tgtgcagctc gcccaggatc tgatcgcgca cgaggcgatc
     3061 ggtgcggcag gcgcgcaatg ccgtgtcctc cacggtaaac acggacgatt gccagccgct
     3121 gtcgagcgcg cactccacgc gcacctccca gcgctcggcg cgaggttcca cttcgaagag
     3181 gtaagcagtc agccccagtt gctccagcag aatgcccacc gtctccttcg cttcggccag
     3241 atttttggcg gtcaggtcca tagtcggctc ctcagttcac acgcacaaaa gggtaacgcg
     3301 cgcgatcttc gcgtttccac agcgacagac cggcccacgg accgatcgtt tccaccgcga
     3361 tcactgcatc gggatccgcg aagcggattt tccccggcgc ctcggttcgg gcgagctgtt
     3421 ccagcagaaa gacatccaac aactgctctt cgcgctggct ggccagcttc tggtgaaaac
     3481 cacggcggtg catgcggaca taaaacgtct tgccgagaag gtcgggaaca aaaccggcgg
     3541 cggcctcctt gacgcgagcc tcgaattcct ccgccgactg gaaggcgaag gagcgggtcg
     3601 ccgggatcac gcgtgccaga cagcgggcca gcgcggaatc ctgttccgtc gtttgtcgca
     3661 gggcgtcgag aaaaccctga acgtcttcga ccttgagcgc gaatacgttg taatagtccg
     3721 tgtggtccac cctcccgaag gcctggagca gcttgtgcgc cagcttgtag cagtcctcgc
     3781 gaatgcttac caccacgttc caatcgagca tgtcccgatc tcagcgtttc cacgccacgt
     3841 gcaggatgtt ggcctcgtcc gtgtactggt agcagagttc acccttgtag gcgtcgtgca
     3901 cggcatcgcc gatgacgcgc gccatgccca tcgtggtcgt ggtgatcaga atgtcgtcgc
     3961 cctgcttatc gatggacatg atgcgccgca gcggatgttc cttcttttcc cgcgcctcgt
     4021 tgtttttcgc cagacgcagg atttcgtcac catgctgttt ctggaacggg ccgctcagcg
     4081 tgaggtaacc gccggggtat ttgtcatggg ttcgatggca tgccggacac agggtttcgt
     4141 gcgcgccttt cggtttcttc agccattgcc agcgaccttt gtggaatacc gcgccgcatt
     4201 cgctgcacac ggtgggctcg cgcagcttgc ccttggtctt gtaggtgtcg tgttcgaatt
     4261 ccttgatata ccggtcgcgg cgtatgcggc gggggctggt gctgactttc cgagcggcgg
     4321 ttcccatggc gtcatcccct ttgtcgttgt tgctgtgata ctgtaaccat gctccggggc
     4381 atgggcaccc gctttgatgc aggtcaaagc tacaactacc ggcagaaaat gcagcgcagg
     4441 agataaccac catgaatcag cgggaatcac aacgacgcct ggccgaggtg gtacgcgatg
     4501 cctgcggcaa agccgcgcag gaggcctacg aaaacgccgg catcagcggg ctgtgcgagg
     4561 aagggcggtg ggaatgcgcg gtcgcggcca tccgcgcgct tgacgtggag gctgttatca
     4621 acaggttacc ggacgatctt tcgaaataac tggagatgct aatccagatt tattgcccgc
     4681 agcttttccg ccgccatttc cggtgcgacg gtattcacaa acagacctga acccagctcg
     4741 aaacccgtgg gaatgctggt acgcggcgtg atttcgagat gccagtgata actcgcctcg
     4801 cagtcctcgc actgaacgtc atgcggcagt gaatgcagga agtagttgta ctgcgcgccg
     4861 ccgagcacgg catcgagccg cgccatggtg cgacgcatca cgcgcgcgag atcgtcgaga
     4921 tcgcccttcg agatgcgcag aaaatcgctc tggtgcgcga gcggcaggat gtgcacctcc
     4981 cattcgtagc ggctggcgaa gggtttgatg gcgatgaatt ttcccacgcg ctcgatgacg
     5041 tactggccga cgttgatctt gcgccggatt tcccccgatt cacggtcgta gatggtggcc
     5101 tcgaaggtaa gcgcctcgtc gatcagggaa cagaaaatac aatgatggtt cttgtgataa
     5161 aacgcgcggc tgttcgcaac ctctgtctgc acgttgtcgg gaatgaccgg cgtggcgatg
     5221 acctggctgt gggtgtgcgc gatcgaggcg ccggcggcgg ggccgaaatt cttgaacgcc
     5281 agcacgtaac gcaggcgtga gttggactta taaagctgtt ccatgcggtc gcggtaggcg
     5341 ccgaacaaca gcgccagatg cgcttcgctc atctcgtgaa tggcgatgcc gtgctggttg
     5401 tggtcgatca tgacctcgtg ccggccatag ccgtcgatag tctgctgcaa tccaaaggtg
     5461 agattggaat tctggcgatc gtcgcccagc accggataca gattctcgac gatgcgtatc
     5521 tcccagtcct tctcggccgg ataggcggcg atgacgggcg gcgtcatgtg ttcgttgccg
     5581 gtacagaacg ggcaggtctc gacgtgggcg cgggtgtcgc gcggcgcggg cgcttcggcc
     5641 tttttcgggc gcatgccgcg cgcggtcgcc accagcaccg actc
//
LOCUS       DBNGZE010000170        15494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_163925, whole genome shotgun sequence.
ACCESSION   DBNGZE010000170 DBNGZE010000000
VERSION     DBNGZE010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 15494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15494
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_163925"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..887
                     /gene="folE2"
                     /locus_tag="AC16FV_04410"
     CDS             66..887
                     /gene="folE2"
                     /locus_tag="AC16FV_04410"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR2849342.1"
                     /translation="MKLKSKTHPDEEVIADVQNTPDLRHLPIDKVGIKDIRHPVLVKD
                     RSQGVQHTIASFNMYVELPHNFKGTHMSRFVEILNSHDMEISVESFKDMLAEMARRLE
                     AHTGHIEMSFPYFINKAAPISGVKSLMDYQVAFVGEITGGKDVMTIKVVVPVTSLCPC
                     SKEISEYGAHNQRSHVTLTVRTNTFVWIEDLIDLVENQASCQLYGLLKRPDEKYVTEK
                     AYENPKFVEDMVRDVAAKLNADDRIDAYVVESENFESIHNHSAYAMIRKDKTRDI"
     gene            complement(924..1000)
                     /locus_tag="AC16FV_04415"
     ncRNA           complement(924..1000)
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_04415"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            complement(1062..2516)
                     /locus_tag="AC16FV_04420"
     CDS             complement(1062..2516)
                     /locus_tag="AC16FV_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849343.1"
                     /translation="MRKAIATILSGVLLGASSAQALDHVWIVGGGPYPGNSQAQIEFN
                     VNWVIQVLNTGAGARQLHVYYTDGSSPGRDVVAWQAPAESKSSLQPLARVFGEQGPNG
                     ERFYSHRVPKVLGDTEATDLKAHLEKEFSELKPGDRALLIYNGHGLQDQGDPAGNTLR
                     LWNDSKLSVREMDQIMSRIDPAVPTRFIFTQCFSGGFARLMHSQAQDTLALGEANRCG
                     FFAESEDRESEGCSASIKVGDYRDYSTYFFAALNGRTRTGEAIGINPDRNGDNVVSLY
                     EAHLFALSQAHNADLPRSTSEVFLERWQPWYLRWIDTGAEPDNVYGRTARELAKKNGL
                     PESGRALIEEMRARRNAMTQKLAELEKEHASLGQEVKALQKEIRKELGLRWPEALSPY
                     TLNFVQFLRKDLDAAQDFILSHPRYPELVTRQDRRAALEFEEISNLDRDITQLDKILR
                     LRNLARLQSQFERHATEEERGWYQRLQSCEGQPL"
     gene            complement(2522..2974)
                     /locus_tag="AC16FV_04425"
     CDS             complement(2522..2974)
                     /locus_tag="AC16FV_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849344.1"
                     /translation="MKTVFLMLKKRKRAKSALILALLLAPLIAYTVYMLALFLLDSFR
                     GDGLMLYQYEFESRRRLALQTLSDGRQALPVFYAVGLLLWLEIHLLSRFGDWNGVLPA
                     AMAGALTGFAVAVIFVEMNWGVVVPAVTAGLLMSLLLAWAIRPSGSSS"
     gene            complement(2971..4653)
                     /locus_tag="AC16FV_04430"
     CDS             complement(2971..4653)
                     /locus_tag="AC16FV_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR2849345.1"
                     /translation="MNILGLNYFFHDSTACLLVDGRMAAVIEEERLTRNKHTGAFPEK
                     AIQRCLDMYHFKSSDIDAVAVSIKPSKDWFTKTVYGLSHITYARPFINHEVIRAYYKQ
                     RSLWDWYKKMWPSGGPKVHFVPHHESHAAGSFLVSPYESAAILSLDGSGEWATSFLGE
                     GEGTRVRCFQQSYFPMSLGSIYEAATEFCGFKPNYDEGKTMGLAPFGDPKPFQKIVSN
                     IVKVDEDGSIKIDLSYFNYQFWGYRRCSQKFYDTFGQPRKGKDFADNHHNVAAAFQHV
                     LEERALELCAILRKKTPHRHLIISGGVALNSVMNGRIVRESGFEDIYVMPAAGDNGTS
                     IGAAYYVYHVVHGHPRIQVHDDPYLGTSYSDDEIGKLIRECKLTAVKHNDIAGEAARL
                     LAEGNILGWFQGRMEIGPRALGNRSILANPTLPYMKDKINAEVKHREAYRPFAPSAIV
                     EKQSVYFDSIGDSPFMLKVCPVRPEMRDKLPAITHVDGSARLQTVSKDINPMYHDLIS
                     KFGEKTGVPVLLNTSFNIMGEPIVESPINAIRCFFSTGLDALALGSYLIRKT"
     gene            complement(4765..5949)
                     /locus_tag="AC16FV_04435"
     CDS             complement(4765..5949)
                     /locus_tag="AC16FV_04435"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2849346.1"
                     /translation="MIELHQEALSCEGGAGEISLAFHRGAQGFAGLREPWRQIDNSLS
                     GKRFFHLYSWYRGYIECLEHDPESVLFCVMSRGSSPIAIFPLKSITRKVCGIPLRVLE
                     LPTHDHIDLGDFIFAKTEDNATLVRTLVRHLRRASGIGWDMIHIPHALEDSATAFALQ
                     HAPPPLWRSTPAGKSNYTDCAMSYDLVATNFGRHFWRNIRRLGRRARDMGALQFHSYC
                     TVEDINRHFHELLQVEAAGWKGEGGTNTAIACDERIQRFYKNLVDGFGPSGQCMLNLM
                     TLNGKCIAGQLCLVVDGTVYILKIGFDETYAAIAPGNLLMEQLFHQCADDKQINAISF
                     VTGRDWNLLWGAKSLRVYDHQIFNRASPRGWVAYLLCRMKDLARSLARFSGLKRNNPA
                     TT"
     gene            complement(6137..8335)
                     /locus_tag="AC16FV_04440"
     CDS             complement(6137..8335)
                     /locus_tag="AC16FV_04440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2849347.1"
                     /translation="MPRDHNTIGRFRIPRPAGLDRLWQSVRRIGERSLVSRLLALLML
                     GAVVVYLIVNVGLWWTASNLIDDNLEKQAVRWLSELDELGTPLYASQGGRHVSNIDKR
                     IKHFPEIAFIRYYDASGKKVLGEFGDRRDVNIPPLTSAQIERLAGTGGADRNYLVDRS
                     ILSGAYLRFISPVSVRSIRSDGLLGFSLENEKPENVKIIGYIDLSIDSGYYKEQLVRS
                     IASGSLFIAALMAMGLFFGSRIIRKALAPLTALQVPLARLAQGETDVNVERAKDSEIA
                     VISDALNTTIRALRERDEALRKMAESDPLTGLVNRSYFLRELEKEIEHASHDAVPSAV
                     FFIDLDQFKYVNDTLGHAAGDKLLIRVAELLKSRVRDKDVVSRFGGDEFTVLARGVSR
                     EDAMDMARSFNQIMRDMRLVEKDKILSVNCSIGIAMIEPGRYSAEEVLAHADMACFDA
                     KSHGRNRMHMYEEAGTGKKEMAMDIGWFQHIKQIIEQDRFRLMYQPIADVMHPDKESY
                     EVLLRMPGPNGELVLPSIFLPVAQRFGLMTDIDRWVIIHALKALAEFRASGRDIIFSI
                     NLSGQSLEDASMLQLIKDHLAQNRLPPSRVIFEITEQSAMRYLDKAQRLIQGLVDIGC
                     RFALDDFGTGFSSFGYLKQLPVDFIKIDGSFVKNMAQNAIDETMVLSIVQIARSLGKQ
                     TVAEFVQDEKTSAMLKASGVDHLQGNYVGVPSEKLPVTLRVVSSAKKNAS"
     gene            complement(8325..9242)
                     /locus_tag="AC16FV_04445"
     CDS             complement(8325..9242)
                     /locus_tag="AC16FV_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2849348.1"
                     /translation="MQHSCFHLRPVWTTWLLSLAALVTGCSTMQPNPGIAILMSDRSP
                     AFVGVQREIEKRYSQRIENYYLDGNEAASSAVQKKIQSSDMPLVVSIGLPAAQVARGL
                     SGKRVVFCQVFNYEDTDLVTTWMKGVAATPPVNEQFRVWKQLSPQLKRVGVITGKNLR
                     GLMDEAQAAARENGLELVHIEALSDKETLYAYKQLLPKIQGLWLVPDNRVLSRDVIRD
                     VMAYSVREGKQVAVFSHQLLALGGLLSAESSYADIAQQVLERVKQAYKDTSEPVMPLS
                     GATIRINTVMAMRLNLTLPKTLQGLAHAP"
     gene            complement(9450..9953)
                     /locus_tag="AC16FV_04450"
     CDS             complement(9450..9953)
                     /locus_tag="AC16FV_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR2849349.1"
                     /translation="MNQRILIIGIGSPFGDDRFGWEAAQSLRHSAIMSAVTPSRIEIL
                     VLDRPGAMLPMHWREADIVILLDAVRSGAAPGTRHCLEAHDLPGTSALYSSHGFGIAS
                     AIGLARALGNMPSRLLLRGMEADPAWADFDVSPAITAALPSFVADIAEEALVLAGFHV
                     SLVSYGT"
     gene            complement(9950..11254)
                     /locus_tag="AC16FV_04455"
     CDS             complement(9950..11254)
                     /locus_tag="AC16FV_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR2849350.1"
                     /translation="MTESRHIPIQVPVLARVEGEGTLELRVHNGVIEDLRLRIFEPPR
                     MFEKLLEGRGYDEIPDMVARICGICPVAYQMSAVHAIESLFGVDPGPWVRAMRRVMYC
                     GEWIQSHSLHIHMLAAPDFLGYSSVVEMAGKYAKEVRRGLKLQALGNDIIRLLGARSV
                     HPVGARVGGFHHAPSREQVNLLREKLREALPEAEDLARWTAALDLPLDEQDFTCVALR
                     HSHEYPMNEGRLVSNHGLDIAVDQFETRFTEQQAPHSTALHCLLDGKPYLVGPLARLN
                     LNLDRLSDPVRKLVNGLDIKFPSRNMHHSIVARAIEIVIAIREATDLLESCQEPQKPY
                     VDIQPRAGSGVGCTEAPRGILWHRYALDAEGKVVRARIVPPTSQNQSRIEDDLRQSLQ
                     SFGLNQPEQALRRRGEMVIRNYDPCISCATHFLDLRINRRPA"
     gene            complement(11247..12053)
                     /locus_tag="AC16FV_04460"
     CDS             complement(11247..12053)
                     /locus_tag="AC16FV_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR2849351.1"
                     /translation="MNSKLETKNSKPRISIHKFASCDGCQLAFLNLGEDLLALAQMVD
                     IVHFAEAGPVDPEATVDIAFIEGSISTPEDVERIQRIRANSRYLVTIGACATAGGVQA
                     LRNFANASEWIASIYTKPEYIQSLATSTPIARHVKVDLEIWGCPVNGRQVLAAVRSLL
                     SGVSPWMEKDKVCMECKRRQTVCVMVAKGIPCMGPVTRTGCGALCPSFGRDCYACYGP
                     AENLNTESLARRLEGLGLVPTAVVRRFRFINAAAPAFADAGSRRGKISHD"
     gene            complement(12050..12439)
                     /locus_tag="AC16FV_04465"
     CDS             complement(12050..12439)
                     /locus_tag="AC16FV_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2849352.1"
                     /translation="MATFRSVEDIDAWKKARHLTDVIYRLTKSGPFSKDFALRDQVRR
                     ACISIMSNIAEGFERDGAGEFAQFLSMAKGSVGELRSQLYIALDQKYIDTSTFKDLTE
                     LCLEISKMIRGLMVYLRKSSIRGAKYK"
     gene            complement(12489..13322)
                     /locus_tag="AC16FV_04470"
     CDS             complement(12489..13322)
                     /locus_tag="AC16FV_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR2849353.1"
                     /translation="MVNASLPHEVEVVERIQESPTIFTLRLRFTDPAQHKAFRFAPGQ
                     FNMLYLYGVGEVAISIVSDPDDEHLFDHTIRVVGRVTRGLAQLKAGDRLGARGPYGRG
                     WPLKQAQDRDVMVITGGLGCAPVVSVINYVIRRRENFRRLVIMQGVKHSDDLIWRNQY
                     AHWSKQRDTQVLVAANVGASLWPWHVGNVTDFFSKVEFDPVRAMIMMCGPEGMMRAAM
                     DHLLERGVHENNIYLSMERSMNCAVGLCGHCQYGGKFVCKDGPVFSYSEAKPLFGMRG
                     F"
     gene            complement(13315..14421)
                     /locus_tag="AC16FV_04475"
     CDS             complement(13315..14421)
                     /locus_tag="AC16FV_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2849354.1"
                     /translation="MEAGFLPRAGFQALLDALHQAGYRCVGPRVRDGAIVYDALGKTG
                     ELPQGIHDRQGPGQYRLEKTASPRCFSWANGPQALKPFLFTPHEPMWRSERNARGEIR
                     FSEIKPAAESLAIIGVRACDLAALRLQDQHFLQGAFVDPYYAARRKNIFLVAVHCTHP
                     ADTCFCASTGDGPRATQGFDLALSELDEGYLVQAGSDMGSTIAAKLPLVPATDIQRRT
                     ADQEAQEAVRCQTRGLPSRNLRDALFANLDHPRWENVAERCLSCGNCTSVCPTCFCHS
                     EMDETALNGGHSMHARQWDSCFTQGHSYIHGMTIRADTRTRYRQWLTHKLGSWHDQYG
                     RSGCVGCGRCIAWCPVGIDITEEAREICGGGGHG"
     gene            complement(14481..14999)
                     /locus_tag="AC16FV_04480"
     CDS             complement(14481..14999)
                     /locus_tag="AC16FV_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849355.1"
                     /translation="MKRHCAWVADASLFCQFQRWPVWVFLLILAFFISACSSGQDSPE
                     AQVRAFLAQGETAAEKKESGVLRQMISERYSDSQGQDKKTVESILRYYFLRNESIHLF
                     TRIQQITIPQPDAAQAIVMVAMAGQPIVDARELERLRADLHRFEITLARENGEWKVTR
                     AEWRRAELADFL"
     gene            complement(15019..15390)
                     /locus_tag="AC16FV_04485"
     CDS             complement(15019..15390)
                     /locus_tag="AC16FV_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HIR2849356.1"
                     /translation="MLHKSATLTVLTTLLVLGSGCTREVSFKQDVFPILSENCLFCHK
                     AGGEGLVKSGLNMESYDGLMKGTKLGPIVVPGSAVGSTLVLLIERQAHPSINMPKDKP
                     PIAAGQIKTIRQWIDQGAKNN"
BASE COUNT         3293 a         4703 c         4383 g         3115 t
ORIGIN      
        1 aaacttgact gtatcactca actactacat tattggcggc ccgcgagtgg tagagagacc
       61 cggttatgaa actcaaaagc aaaacccatc ccgatgaaga agtcatcgcc gacgtgcaaa
      121 acacgccgga cctgcgccac ctgccgatcg acaaggtcgg catcaaggac atccgccatc
      181 cggtgctggt caaggaccgc tcgcagggcg tgcagcacac catcgccagt ttcaacatgt
      241 acgtggaact gccgcacaac ttcaagggta cccacatgtc gcgcttcgtg gagatactca
      301 acagccacga catggaaatc tcggtcgagt ccttcaagga catgctggcg gaaatggcgc
      361 ggcggctgga ggcccatacc ggccacatcg agatgagctt cccctacttc atcaacaagg
      421 cggcgccgat ctcgggggtc aagagcctga tggattacca ggtcgccttc gtcggcgaga
      481 tcacgggcgg caaggacgtc atgaccatca aggtggtggt gccggtcacc agcctgtgcc
      541 cctgctccaa ggagatttca gagtatggcg cccacaacca gcgctcgcat gtcacgctga
      601 cggtgcgcac caataccttc gtgtggatcg aggatctgat cgacctggtg gaaaaccagg
      661 ccagttgcca actttacggc ctgctgaagc gcccggacga gaaatacgtc accgagaagg
      721 cctacgagaa tcccaaattc gtggaagaca tggtgcgcga cgtcgcggcc aaactgaacg
      781 ccgacgaccg catcgacgcc tacgtggtgg aatcggaaaa tttcgagtcc atccacaacc
      841 actcggcata tgccatgatc aggaaagaca agacgcggga tatctgaggt agggtttacc
      901 cgcccgtcct aaagacagaa gctgtctcaa aaacgtcgcg agcggccgtc agccgggtga
      961 ggacggagcg caggaaccgg agtgtacacg gtaagtacat gaggattccg agcaccgccc
     1021 gatcccggat cacggacgcg cagcaggttt tgggacagct tctaaagagg ttgtccctcg
     1081 caagattgca accgctgata ccacccacgc tcctcttccg tggcgtggcg ttcgaactgg
     1141 ctctgcagac gtgccagatt ccgcagacgc agaatcttgt cgagctgggt gatgtcccgg
     1201 tccagattcg atatctcctc gaactccagc gcggcgcgcc ggtcctgcct ggtcaccagc
     1261 tccggataac gcggatgcga caggataaaa tcctgcgcgg cgtccaggtc tttcctgagg
     1321 aactggacga aattcagggt atacggcgaa agcgcctcgg gccagcgcaa gccaagttcc
     1381 ttcctgattt ccttttgcag cgccttgacc tcttggccca ggcttgcatg ctccttttcc
     1441 agctccgcca atttttgcgt catcgcattc cggcgcgccc gcatttcctc gatgagcgca
     1501 cgccccgatt cgggcagacc gttctttttc gccaactcgc gcgccgtcct gccatacacg
     1561 ttgtccggct ccgcgccggt gtcgatccag cgcagatacc acggctgcca gcgttcgaga
     1621 aacacctcgc tggtggaacg cggcagatcg gcgttgtgcg cctgtgacag cgcgaacaga
     1681 tgcgcctcat aaagcgacac cacattgtcg ccattgcgat ccggattgat gccgatggcc
     1741 tcgccggtgc gcgtgcggcc gttgagcgca gcgaagaaat aggtcgagta gtcacggtaa
     1801 tcgccgacct tgatgctggc cgaacatccc tccgattcac ggtcctccga ttcggcgaaa
     1861 aacccgcagc gattggcctc cccgagcgcg agcgtgtcct gcgcctgcga atgcatcagc
     1921 cgcgcgaagc cgccggagaa acattgcgtg aatatgaagc gcgtgggcac agcggggtcg
     1981 atgcggctca tgatctgatc catctcgcgc accgagagtt tgctgtcgtt ccataatcgc
     2041 agcgtgttcc ccgcgggatc gccctggtcc tgcaacccgt ggccgttgta gatcaacagc
     2101 gcccggtcgc cgggcttgag ttcggaaaac tccttctcca gatgtgcttt gagatcggtc
     2161 gcctcggtgt cgccaagaac tttgggaacc cggtggctgt aaaaacgctc gccattcggg
     2221 ccttgttcgc cgaaaacacg cgccagcggc tgcaacgagc ttttggattc cgcgggagcc
     2281 tgccacgcaa cgacatcccg gccgggactg gaaccgtcgg tgtaatagac gtgcaactgg
     2341 cgcgcgccag cgccggtatt caacacctgt ataacccagt tcacattgaa ttcgatctgc
     2401 gcctgcgagt tgccggggta gggcccgccg ccgacgatcc agacgtgatc cagggcctgg
     2461 gcggacgagg cgcccaaaag gacaccggaa aggatggtcg cgatagcctt gcgcatggcg
     2521 atcaagacga cgagccggaa ggtctgatgg cccaggcaag caacagcgac atcaacaggc
     2581 cggcggtgac ggcaggaaca acgacacccc agttcatctc cacgaagatg acggcgacgg
     2641 cgaaaccggt cagcgcgccg gccatggcgg ccggcaacac gccattccag tcgccgaacc
     2701 gcgacaacaa gtggatctcc agccataaca gcaaacccac cgcatagaac accggcagag
     2761 cctgacggcc gtcggacaaa gtctgaagcg ccagccggcg gcgcgattcg aactcatact
     2821 ggtacaacat cagtccgtcg ccacggaagc tgtccagcag gaacagcgcc agcatgtaca
     2881 ccgtgtatgc aatgagaggg gccagcaaca aggcaaggat gagcgcactt tttgcgcgct
     2941 tccttttttt cagcatgaga aaaacggttt tcaagtcttc ctgatcagat agcttcccag
     3001 cgccagcgcg tcgagcccgg tggagaagaa acaacggatc gcattgatgg gtgactccac
     3061 aatcggctcc cccatgatgt tgaagctggt gttcaacaat accggtactc cggtcttctc
     3121 gccgaacttg ctgatcaggt catgatacat cggattgatg tctttgctca ccgtctggag
     3181 gcgggcggaa ccgtccacat gcgtgatcgc cggtaatttg tcgcgcatct ccggtcgcac
     3241 cgggcatacc ttcagcatga agggactgtc cccgatggag tcgaaatata cactttgctt
     3301 ctccacaatc gcggaggggg cgaacggccg gtaagcctcg cggtgtttga cctcggcgtt
     3361 gatcttgtcc ttcatgtacg gcagcgtcgg attcgccaga atactgcggt tgccgagcgc
     3421 ccgcggcccg atttccatgc ggccctggaa ccagccgagg atattgccct cggccagcag
     3481 gcgcgcggcc tcgccggcaa tatcattgtg cttcaccgcg gtcagcttgc actcgcgaat
     3541 cagcttcccg atctcgtcat cggaatagga ggtgccgaga tagggatcgt cgtgaacctg
     3601 gatacgcgga tgcccatgca ccacgtgata gacataataa gcggcgccga tggaggtgcc
     3661 gttgtcaccc gcggccggca tgacgtagat gtcctcgaac ccgctttccc gcacgatgcg
     3721 gccgttcatg acgctgttca gcgccacgcc gccggagatg atgagatggc ggtgcggggt
     3781 ctttttgcgc aggatggcgc acagctccag cgcccgctcc tccaggacat gctgaaaggc
     3841 agcggcgaca ttgtggtggt tatccgcgaa atccttgcct tttcgcggct gtccgaaggt
     3901 atcgtagaac ttctgggaac agcgccggta gccccagaac tgataattga agtaagacag
     3961 gtcgattttt atcgagccgt cttcgtctac tttcacgatg ttcgacacta ttttttgaaa
     4021 aggtttggga tcgccgaagg gcgcgagacc catggtcttg ccttcgtcgt aattcggctt
     4081 gaagccgcaa aattccgtgg ccgcctcgta gatggacccg agcgacatgg gaaaatagct
     4141 ctgttgaaag catctgactc gggtgccctc gccttctccc agaaacgacg tggcccattc
     4201 gcccgagcca tcgaggctca ggatcgccgc gctttcatag ggagaaacca gaaagctccc
     4261 ggccgcgtgc gactcgtggt gcggcacgaa atgcactttc gggccgccgg agggccacat
     4321 ttttttgtac cagtcccaca gcgagcgctg cttgtagtag gcccggatga cctcgtgatt
     4381 gatgaacggg cgcgcgtagg tgatatgcga cagaccgtaa acggtcttgg tgaaccagtc
     4441 tttgctcggc ttgatggaga cggccaccgc gtcgatgtcc gaggacttga aatggtacat
     4501 gtccaggcag cgctgaatgg ccttctccgg gaatgccccg gtgtgcttgt tgcgcgtgag
     4561 ccgctcctcc tcgatcacgg ccgccatgcg tccgtccacc agcaggcagg ccgtggaatc
     4621 gtgaaagaaa taattaaggc caagaatatt cattgtcaga tttcccggtt cagtcagttg
     4681 taaatttctt gtcgccagat ccggcaacgg gttgtgataa atgtcgccct acttcgttcg
     4741 tctggacata agaataggta tgactcaggt cgtcgccgga ttatttcttt tcaatcccga
     4801 aaatctggcc aacgagcgcg ccaggtcctt catgcggcac agcagatacg cgacccagcc
     4861 acgcgggctg gcgcggttga aaatttgatg gtcgtatacc cgaagcgact tggcgcccca
     4921 cagcaaattc cagtccctgc ccgtcacgaa acttatcgca ttgatctgct tgtcgtccgc
     4981 gcattggtgg aataactgtt ccatgagcag gtttcccggt gcgatggcgg cgtaggtctc
     5041 gtcaaaaccg atcttgagaa tgtagacggt gccgtcgacc accagacaca gttgtccggc
     5101 gatgcatttg ccgttgagtg tcatgagatt gagcatgcac tggccggagg gaccgaatcc
     5161 gtccaccagg tttttataga agcgctggat tcgctcgtcg caggcgatcg ccgtgttcgt
     5221 tccgccttct cccttccagc cggcggcctc gacctgcagg agttcgtgaa aatggcggtt
     5281 gatgtcctcg acggtgcagt aactgtgaaa ttgcagcgcc cccatatcgc gcgcccggcg
     5341 gcccagtcgg cggatgttgc gccagaagtg tctgccgaaa ttggtggcga ccaggtcata
     5401 gctcatggcg caatcggtgt aattgctttt cccggcaggc gtggaccgcc ataacggtgg
     5461 tggagcatgt tgcaaggcga aggctgtcgc cgaatcctcg agcgcgtgag ggatgtgaat
     5521 catgtcccag ccgatgccgg aggcccggcg caagtgccgt accagcgtcc tcaccagtgt
     5581 ggcgttatct tcggtcttgg caaaaataaa atcgcccaag tcgatatgat cgtgggttgg
     5641 aagctccaga acccgaagcg gaataccgca gaccttccgc gtgatgctct tcagggggaa
     5701 tatcgcgatg ggcgacgatc cgcgcgacat gacgcaaaac aacaccgatt caggatcgtg
     5761 ctccaggcac tcaatgtaac cgcgatacca ggaatagaga tgaaaaaacc gctttccgga
     5821 gagtgaatta tcgatctgac gccacggctc gcgcagtccg gcgaaacctt gcgcgccacg
     5881 atgaaacgca agcgagattt cacccgcccc accctcgcat gagagcgctt cctgatgcaa
     5941 ttcgatcatt tgattctggc tctttttacg ggaccgaatg atggcggcgt gctggcacac
     6001 cccgccctcc cctcaaaaat gctgtcgcct gttttgtttt tatgataaca caggcttgga
     6061 atcccgcgga aaacccgctg gaaagccctt tcgccgcggt tcgcacttcc ctgtgccgac
     6121 gccatcctta cccccttcag ctcgcgttct ttttggccga cgagaccacg cgcagcgtca
     6181 ccgggagctt ctccgacggt acgcccacat aatttccctg cagatgatcc acgcccgagg
     6241 ccttgagcat ggcgctggtc ttttcgtcct gcacgaactc ggcgaccgtc tgcttgccga
     6301 gcgagcgcgc gatctggacg atcgagagca ccatggtctc gtcgatggcg ttttgtgcca
     6361 tgtttttgac gaaggacccg tcaatcttga tgaaatccac cggcagctgt ttcagatacc
     6421 cgaaggaact gaagccggtg ccgaaatcgt ccaacgcaaa gcggcagccg atatccacca
     6481 gcccctgaat gaggcgctgc gccttgtcga gatagcgcat ggccgattgc tcggtgatct
     6541 cgaaaatcac ccggctggga ggcaggcggt tctgcgccag atggtccttg atcaactgga
     6601 gcatggacgc atcctccagc gactggcccg acagattgat cgagaagatg atatcgcgcc
     6661 ccgaggcacg gaactccgcc agcgccttga gcgcatgaat gatcacccag cggtcgatat
     6721 ccgtcatcag gccgaagcgc tgtgccaccg ggaggaagat ggagggcaac accagctcgc
     6781 cgttggggcc aggcatgcgc aacaatacct catagctttc cttgtcggga tgcatgacat
     6841 cggcgatagg ctgatacatc agccggaaac ggtcctgctc gatgatctgc ttgatatgct
     6901 ggaaccagcc gatatccatg gccatttctt tcttgccagt gcccgcctct tcatacatgt
     6961 gcatgcggtt gcggccgtgc gatttggcgt cgaagcacgc catgtctgca tgcgccagca
     7021 cctcttccgc ggaatagcgt ccgggctcga tcatggcgat gccgatgctg caattgacgc
     7081 tgagaatctt gtccttctcc accaggcgca tgtcacgcat gatctggttg aacgatcgcg
     7141 ccatgtccat cgcgtcctcg cgggaaacgc cgcgggccag cacggtgaac tcgtcgccgc
     7201 cgaagcgcga caccacgtcc ttgtcgcgca cgcgcgactt tagcagctcg gccacgcgga
     7261 tcagcagctt gtcgcccgcg gcatgcccga gcgtgtcgtt gacgtacttg aactggtcga
     7321 gatcgatgaa gaacaccgcg ctcggcacgg cgtcgtgcga cgcgtgctcg atctcctttt
     7381 ccagctcgcg caggaaatag ctgcggttca ccagacccgt caatgggtcg ctttccgcca
     7441 ttttgcgcag cgcctcgtcg cgctcgcgca gggcgcggat ggtggtgttg agcgcgtcgc
     7501 tgatcaccgc aatttcagag tctttcgcgc gctcgacgtt gacgtcggtt tcaccctgcg
     7561 ccagccgcgc gagcggcact tgcaatgccg tcagcggcgc cagcgccttg cggataatcc
     7621 ggctcccgaa gaacaatccc atcgccatca gcgcggcaat gaacaggctg ccggaggcga
     7681 tgctgcgcac caattgttcc ttgtagtaac cggaatcgat gctgagatcg atgtaaccga
     7741 tgatcttcac gttctccggt ttttcgtttt ccagactgaa accgagcagg ccatccgaac
     7801 ggatggaccg gacacttaca ggagagatga atcgcagata ggcaccgctc aggatggagc
     7861 gatcgacaag ataattcctg tccgctccgc cggtgccggc cagtcgctcg atctgggcgg
     7921 acgtgagcgg cggaatattc acgtcacgac gatcgccgaa ttcgcccagg actttcttgc
     7981 ccgaggcatc gtaataacgg atgaaggcaa tttccggaaa atgcttgatg cgcttgtcga
     8041 tattcgacac atgccggccg ccctgcgagg catacagcgg cgtgcccaac tcatccagct
     8101 cgctcagcca gcggaccgcc tgtttctcga gattgtcgtc gataagattg ctggcggtcc
     8161 accacaggcc gacattgacg atcagataga ccaccacggc gcccagcatg agcagcgcca
     8221 gcaggcggct cacgagactg cgttcgccta tgcggcgaac cgattgccat aacctgtcca
     8281 ggccggccgg gcgcgggatg cggaaacggc caatggtgtt atgatcacgg ggcatgggcc
     8341 aatccttgca gcgttttggg tagcgtcaga ttcaggcgca tggccatcac ggtattgatt
     8401 ctgatcgttg ccccggaaag aggcatcacc ggctcgcttg tgtccttata cgcctgtttc
     8461 acgcgctcca atacctgttg cgcgatgtct gcgtagctgc tttccgcgct cagaagaccg
     8521 cccagagcca gcaattgatg gctgaagacc gccacctgct ttccttccct gacgctgtat
     8581 gccatcacgt cgcggatgac gtcgcgactc aacacacgat tgtccggcac cagccacagg
     8641 ccctggattt tcggcagaag ctgtttgtag gcataaagcg tctccttgtc cgacagcgct
     8701 tcgatgtgaa ccagttccag accgttctcg cgggccgctg cctgcgcttc atccatcagg
     8761 ccacgcaggt ttttgccggt gatgacgccg actcttttaa gctggggcga caactgtttc
     8821 cagacacgaa actgttcatt cacgggcggc gtcgcggcga cacctttcat ccaagtggtc
     8881 accaggtcgg tgtcttcata gttaaacacc tggcagaaga cgacgcgctt tcccgaaagg
     8941 ccgcgcgcca cctgggcggc gggcaggccg atgctcacga ccagcggcat gtcggaggac
     9001 tggatttttt tctgcacggc ggaggatgcc gcctcgttgc cgtcgagata ataattctcg
     9061 attcgctgcg aatatctttt ctcgatttca cgctgcacgc cgacaaacgc cggtgaccgg
     9121 tcgctcatca ggatggcgat gcccggattc ggctgcatcg tcgaacaacc cgtcaccagg
     9181 gccgccagcg acaacaacca cgtcgtccag acagggcgta gatggaaaca ggaatgctgc
     9241 aacagcgcac ctcatagcga ctttatttat acacatttaa tgcagcattc atgccatggc
     9301 ttgggtattt ccaggcagga aatgcccgcg cacaaccacc tgaaacagca gggcttttac
     9361 tggataagga actgcaagcc ggggccagcc tgaaccagcc tcaaaacagg cttgtaacca
     9421 tgggaattcc ctgccggaaa aggtctggct caggtcccat atgagacaag ggacacatga
     9481 aaacccgcga gcacaagcgc ctcttccgcg atgtctgcca cgaaagacgg caaggcggcg
     9541 gtaatcgctg gactcacgtc aaaatccgcc cacgcgggat ccgcctccat gccgcgcaac
     9601 aacagacgcg acggcatgtt gccgagggcc cgggcaagcc cgatggcgga agcgataccg
     9661 aaaccgtgag aggagtacaa cgcgcttgtg ccgggaagat cgtgcgcttc aaggcaatgc
     9721 cgcgttcccg gagcggcgcc ggaacggacc gcatccagca ggatcacgat gtcggcctcg
     9781 cgccaatgca tcggcaacat ggcgccagga cggtccagaa ctaatatttc gatccggctg
     9841 ggcgtgaccg cgctcatgat ggcggaatgg cgcagagatt gcgccgcctc ccagccgaaa
     9901 cgatcgtcac cgaaaggaga accgattccg ataataagta tccgctggtt catgccggcc
     9961 gcctgttgat gcgcagatcg agaaaatgcg tggcacaaga gatgcaggga tcgtaattgc
    10021 gaatcaccat ctcaccacgg cgacgcagcg cctgctcggg ttgattcagg ccgaaactct
    10081 gcagggactg gcgcaaatcg tcttcaatac gcgactggtt ctggctggtg ggcggaacga
    10141 tgcgcgcgcg gacaactttt ccttcggcgt cgagtgcata gcgatgccac agaatcccgc
    10201 gcggtgcctc ggtgcaacca actccgctgc cagcacgcgg ctggatgtcc acataaggct
    10261 tctgcggctc ctgacaggat tcgaggagat cggtcgcctc ccgtatggcg atcacaatct
    10321 cgatcgcacg cgccacgatg ctgtgatgca tattacggct gggaaatttg atatccaggc
    10381 cgttcacaag cttgcgcacg gggtccgata accggtcaag attgagattc aggcgcgcca
    10441 gcggaccgac cagatacggc ttgccatcga gcaggcaatg caatgcggtc gaatgcggcg
    10501 cctgctgttc ggtaaaccgt gtctcgaatt gatccactgc gatatccaga ccatggttcg
    10561 agaccagacg gccctcgttc atgggatatt catgcgaatg acgcaatgcc acacaggtaa
    10621 aatcctgttc atcgagcggc aggtcgagag cagctgtcca gcgcgccaga tcctcggcct
    10681 ctggcagtgc ctcacgcagt ttctcccgca acagatttac ttgctcccgc gacggtgcat
    10741 gatgaaagcc gccgacacgc gcgcccacgg gatgcaccga acgcgcgccg agcaatcgga
    10801 tgatgtcgtt accgagcgct tgcagtttca ggccacgccg tacttccttc gcatatttcc
    10861 cggccatttc caccacgctc gaataaccca gaaaatccgg cgccgccaac atgtggatat
    10921 gcaggctgtg actctgtatc cactcgccgc agtacatgac acgacgcatg gcgcgtaccc
    10981 agggacccgg gtccacgccg aacaggcttt cgatcgcgtg caccgcactc atctgataag
    11041 ccaccgggca gatgccgcag atgcgcgcca ccatgtcagg tatttcatca taccccctgc
    11101 cctccagcag tttctcgaac atccgcggcg gctcgaagat acgcaggcgg agatcctcga
    11161 tcacgccgtt gtgcacgcgc aattcgagcg tgccctcgcc ctcgacgcgc gcgagcaccg
    11221 gaacctggat cgggatgtga cgcgactcag tcatgggata ttttgcctcg gcgcgaaccg
    11281 gcgtctgcaa aggcgggcgc cgcggcattg atgaatcgga accgtcgcac cacggctgtc
    11341 ggcacgaggc caagcccttc caggcgtcgt gccagtgatt cagtattaag attttccgcc
    11401 gggccgtaac aggcgtagca gtcgcgcccg aaactcgggc agagggcccc gcagccggtg
    11461 cgcgtcacag gtcccatgca gggaatcccc ttcgccacca tcacgcacac ggtctgccgg
    11521 cgcttgcact ccatgcagac cttgtctttt tccatccagg gactcacgcc cgacagcagg
    11581 gaacgcactg cggccagcac ctgacgccca ttcaccggac aaccccagat ttcaagatcg
    11641 actttcacat gtcgggcgat gggtgtggag gtcgccaaac tttgaatata ttccggtttg
    11701 gtgtatatcg atgcgatcca ctccgaagca ttcgcgaaat tgcgcaatgc ctgcacgccg
    11761 ccggcggtgg cgcaagctcc gatggtgaca aggtagcgag agttggcgcg gatacgctgg
    11821 atgcgttcaa cgtcctctgg cgttgaaatg ctgccttcga taaaggcgat gtccactgtc
    11881 gcctccgggt ccaccggccc agcctcggca aaatgcacga tgtccaccat ctgtgcgagc
    11941 gccagcaggt cctcgcccag attcaaaaac gcaagctggc agccgtcgca agaagcgaat
    12001 ttatgaatcg aaattcgcgg tttcgagttt ttagtttcta gtttcgagtt cacttgtatt
    12061 tagcgcctct gatagaagac ttacgcaagt aaaccatcaa acctcgaatc atcttactaa
    12121 tttccagaca caattcggtc aaatctttaa acgtactggt atctatatat ttttgatcga
    12181 gtgctatata gagctgagac cttaactctc cgaccgaacc cttggccatg gaaaggaact
    12241 gggcgaattc tcccgcaccg tctctctcaa aaccctccgc gatgttagac ataatagaaa
    12301 tgcaagccct tctgacttga tcgcgcagcg caaagtcctt tgaaaatggt ccagatttcg
    12361 tgagtcgata gattacatcc gttaaatgtc ttgccttttt ccatgcatcg atatcctcga
    12421 cactccggaa agttgccatt cgccctcccc tgaactagaa actaaaaacc agaaactaga
    12481 aacttgaatt agaatccccg catgccgaaa agcggcttgg cctcggaata ggaaaacacg
    12541 ggaccgtcct tgcagacgaa cttgccgccg tattggcagt gcccgcaaag tcccacggcg
    12601 caattcatgc tgcgctccat gctgagatag atgttgttct cgtgcacacc gcgttcgagc
    12661 aaatgatcca tggccgcccg catcatgccc tcgggaccgc acatcatgat cattgcgcgc
    12721 acgggatcga actccacctt gctgaaaaaa tccgtcacgt tgccgacatg ccagggccag
    12781 agcgaggccc cgacattcgc cgccaccagc acctgggtat cgcgttgttt gctccagtgc
    12841 gcatattggt tacgccagat cagatcgtcc gaatgcttga cgccctgcat gatgaccagg
    12901 cggcgaaaat tttcgcgccg gcgaatgaca taattgatta ccgacaccac tggcgcgcag
    12961 ccaagcccgc cggtgatcac catcacgtcc cggtcctgcg cctgtttcag cggccaaccg
    13021 cgcccgtagg gaccgcgcgc gcccagccgg tcgccggcct tcagttgtgc cagcccgcgc
    13081 gtcacgcgtc cgaccacacg gatggtgtga tcgaacagat gctcgtcgtc cggatccgag
    13141 acaatggaaa tcgccacttc gccgacgccg tacagataaa gcatgttgaa ctggcccggc
    13201 gcaaaccgga acgccttgtg ctgcgccggg tcggtgaacc gcaggcgcag ggtgaagatg
    13261 gtgggtgatt cctgtatgcg ctccactact tcgacttcat gcggcagaga ggcattaacc
    13321 atgtccgcct cccccgcata tttcccgggc ctcttccgtg atatcgatgc cgaccggaca
    13381 ccaggcgatg cagcggccgc aaccgacgca accgctgcgc ccatattgat cgtgccagct
    13441 gccgagttta tgcgtcagcc actggcggta acgcgtacgt gtatcggcac ggatggtcat
    13501 gccgtgaatg tagctgtggc cctgggtgaa acaggaatcc cactggcgcg cgtgcatgct
    13561 gtgaccgcca ttgagcgctg tctcatccat ctcgctgtgg cagaagcagg tgggacaaac
    13621 cgacgtgcag ttgccacagg aaaggcagcg ctccgccacg ttctcccagc gcgggtgatc
    13681 gagattcgcg aatagcgcat cgcgcaggtt gcgcgagggc aagccacggg tctgacatcg
    13741 gacggcctcc tgtgcttcct gatcggcagt gcgtcgttgt atgtccgtcg ccgggacaag
    13801 cggcagtttc gcagcaatcg tgcttcccat gtcgctgcca gcctgcacca ggtaaccctc
    13861 atccagttcg ctcagggcaa gatcgaaccc ctgcgtggcg cgcggcccat cgccggtgga
    13921 ggcgcagaaa cacgtatctg ccggatgggt gcagtgaacc gcaacaagaa aaatattttt
    13981 tcgccgggcg gcgtaataag gatcgacgaa cgcgccttgc agaaaatgct ggtcctggag
    14041 acgcaatgcg gccagatcgc aggcgcgtac gccaatgatg gccagcgatt ctgccgccgg
    14101 tttgatttcc gaaaaacgga tttcgccgcg ggcattgcgc tcggaacgcc acatcggctc
    14161 gtgaggggtg aacagaaacg gcttcagcgc ctgcggacca ttggcccagc tgaagcaccg
    14221 cgggcttgcc gtcttttcaa gccgatactg gcccgggccc tggcggtcgt gaattccttg
    14281 cggcagctcc ccggtcttgc cgagagcgtc gtaaacgatc gcgccatctc gcacccgcgg
    14341 cccgacacaa cggtaaccgg cctgatgcag cgcatccagc agtgcctgaa acccggcgcg
    14401 tgggagaaaa cccgcctcca tgctcgcctc gtcggtatac cgtggttgac gacattacat
    14461 cacaattgtc ccacgccgtc tcagagaaaa tcggcgagct ccgcgcggcg ccattcggcg
    14521 cgtgtcacct tccactctcc attctcccgc gccagcgtaa tctcgaatcg gtgcagatcg
    14581 gctcgtaacc gctcaagttc cctggcgtcc acgatcggct ggccggccat ggccaccatc
    14641 acgatagcct gggccgcatc cggttgtgga atggtgatct gctggatgcg cgtaaacaga
    14701 tgaatcgact cattgcgcag aaaataataa cgcagtatcg actcgacggt ctttttatcc
    14761 tgaccctggc tgtcggaata tctttccgaa atcatctgtc gcagcacgcc tgattccttc
    14821 ttctcggccg cggtctcgcc ctgtgccagg aatgcgcgca cctgcgcttc ggggctgtcc
    14881 tgccccgatg aacatgcact gataaagaac gccaggatca acaggaagac ccataccggc
    14941 cagcgctgaa attgacagaa cagactggcg tcagcgaccc aggcgcaatg tcgtttcaat
    15001 catcttctcc cggatgaatc agttattctt ggcgccttga tcgatccact gtctgatggt
    15061 cttgatctga ccggcggcga tcggcggctt gtccttgggc atgttgatgc tgggatgtgc
    15121 ctggcgttcg atcaacaaca ccagcgtgct gccgacagcc gaaccgggaa cgacaatcgg
    15181 gcccagcttg gtgcccttca tcaggccgtc gtaactctcc atattaagcc cgcttttgac
    15241 aaggccctcg ccgccggctt tatggcagaa caggcaattt tcactcagga tcggaaacac
    15301 atcctgcttg aaactgactt cacgggtgca gccgctgccc agaacaagca aggtcgtgag
    15361 aacagtcagt gttgctgatt tgtgtaacat ggctcacctc ccgtttaggt ctcggatatc
    15421 ctcgaccata ttgtttattt acaccacgcc ggtaaccgaa aataaatgac gtgcgtcaaa
    15481 tatgccggac gccg
//
LOCUS       DBNGZE010000171         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_858883, whole genome shotgun sequence.
ACCESSION   DBNGZE010000171 DBNGZE010000000
VERSION     DBNGZE010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_858883"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..929)
                     /locus_tag="AC16FV_04490"
     CDS             complement(174..929)
                     /locus_tag="AC16FV_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR2849357.1"
                     /translation="MKPAALKKALYTSAFLFLCFLAAASVADETATVRHVLDGDTFIL
                     VDGRHVRLIGVNAPELGKDGAPGQPLAKQARNRLSRLVKGQPVTLSYEHERQDHYGRW
                     LAHARLAEGESVEEILLREGLVWAIAIPPNVDQLSSLFMVENEARAAGRGIWSEPAYR
                     PKPAESLTATDTGFRFIEGTVLKRAQGRQVIYFDLAPRVALVVPREEWKKYFDGKSTN
                     LVGRTVVARGWLTEHRGRLHLRVPHPAMLTWRD"
     gene            complement(936..1142)
                     /gene="rpmE"
                     /locus_tag="AC16FV_04495"
     CDS             complement(936..1142)
                     /gene="rpmE"
                     /locus_tag="AC16FV_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR2849358.1"
                     /translation="MKEAIHPAYQEITVNCSCGNSFTTRSTLDHELHVEVCSVCHPFY
                     TGKQKLVDTGGRVEKFRQKYGMKK"
     gene            complement(1294..>1800)
                     /gene="rho"
                     /locus_tag="AC16FV_04500"
     CDS             complement(1294..>1800)
                     /gene="rho"
                     /locus_tag="AC16FV_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151085.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR2849359.1"
                     /translation="KRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLSGGVDSNAL
                     QRPKRFFGAARNLEAGGSLTIMATALIDTGSRMDDVIYEEFKGTGNMEIHLDRRLAEK
                     RSYPSININRSGTRREELLLIPEELQKIWILRKLLHQMDELEASEFLLEKLRATKNNG
                     EFFDSMKR"
BASE COUNT          323 a          518 c          556 g          403 t
ORIGIN      
        1 ggaccttgct gtcgtagctg tcaccgagtt gaatctccct cgcctcggtc atgaacacga
       61 ggtctggcgt tccggtgaca ggattggtgg cgcagccgga gacaaagccg actgttgtgg
      121 cggcaagcag caggggaatc aggaagcttt ggaaaatacg gttacgcatt ggctcagtcg
      181 cgccatgtta gcatggccgg gtgcggcaca cgcaggtgca gccggccgcg gtgctccgtg
      241 agccagccgc gcgcgacaac ggttcgtccg accagattgg tagactttcc atcaaagtat
      301 tttttccact cctctcgggg cacgacgaga gctacgcgtg gggccagatc gaaataaatt
      361 acctgccgtc cttgggcgcg ttttagtacg gtcccctcga tgaaccgaaa tcctgtatcc
      421 gttgccgtga gactctcggc cggttttggc ctgtaggcgg gttcgctcca gatgccgcgt
      481 cccgccgcgc gcgcttcatt ttccaccatg aacaggctgc ttaattgatc cacgttcggc
      541 gggatagcga ttgcccagac gagtccttcc cggagcagga tttcctcgac gctttcaccc
      601 tccgcaagac gggcgtgtgc cagccagcgg ccataatgat cctggcgttc atgctcgtag
      661 gaaagagtca ctggttggcc ttttaccaac cttgagagtc tgttacgcgc ctgtttcgcc
      721 agcggttggc cgggcgcacc atccttgccc agttccggcg cgttaacgcc gatcagacgg
      781 acatgccggc catcaacgag gatgaaggta tcgccgtcga gtacatggcg gactgttgcc
      841 gtttcgtcgg caacgctggc cgcggccagg aagcacaaaa ataaaaaggc gctcgtgtag
      901 agcgcctttt tgagagcagc cggcttcacc ggtgcttatt ttttcatgcc gtacttctgg
      961 cggaatttct cgacgcgccc gccggtgtcc accagtttct gcttgccggt gtagaacgga
     1021 tggcacacgg agcacacttc cacgtgcagt tcgtgatcga gggtggaacg ggtcgtgaag
     1081 ctgttgccac agctgcaatt gaccgtgatt tcctggtaag ccggatggat agcttctttc
     1141 atgtcattga tctcactgga tttccgcccg atttcgcccg aacggacctt gaaagggcgg
     1201 gcattctaag cggaatcaag ggcttgggca agccgcgcgt tcagcgcttc gatttaagga
     1261 caaatcgagg gcgctggggc agggatgccc cgcctagcgc ttcatggagt cgaagaactc
     1321 cccgttattc ttggtggccc gcagcttctc cagcaggaat tccgaggctt cgagttcgtc
     1381 catctggtgc aagagttttc tcaggatcca gatcttttgc agctcttccg ggatcagcag
     1441 cagctcttcg cggcgggtgc cggaacggtt gatattgatc gaggggtagc tgcgtttttc
     1501 ggccagacgc cggtcgagat ggatttccat gttgccggtg cccttgaatt cctcgtagat
     1561 cacgtcgtcc atccgcgagc cggtgtcgat gagcgcggtc gccatgatcg tcagcgaccc
     1621 gccggcctcg aggttgcgcg cggcgccgaa gaagcgcttc gggcgctgga gcgcgttgct
     1681 gtccacgccg cccgacagga ccttcccgga ggcgggtacg acggtgttgt aggcgcgcgc
     1741 gagccgcgtg atcgagtcga gcaggatcac gacgtccttc ttgtgctcga ccaggcgctt
//
LOCUS       DBNGZE010000172         3019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1553538, whole genome shotgun sequence.
ACCESSION   DBNGZE010000172 DBNGZE010000000
VERSION     DBNGZE010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1553538"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="AC16FV_04505"
     CDS             <1..793
                     /locus_tag="AC16FV_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2849360.1"
                     /translation="SFTDAEKKQGYVLLCSHTAVTDLEIEATEIGGAQDIPEQQIATR
                     VKKIEPLGEDMALLHLQTPRTKRLQFLAGQAVTLETANGAEADCPVASCPCDDRNLQF
                     HVQRLPGNRLADYVFKKLKNGDIVALAGPKGSFTLNEESPRSLIFLAHGTGFAPIKSL
                     IEHAMARDVAESLHLYWLSGDGGHYLNNLCRAWTDALDNFHYTPLVADATKSAPALKQ
                     ITKDHPDLGGFDIYTAGPEAFLTSVKKYLHDRNFPENQLITNRVD"
     gene            complement(803..2731)
                     /locus_tag="AC16FV_04510"
     CDS             complement(803..2731)
                     /locus_tag="AC16FV_04510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2849361.1"
                     /translation="MNHRQTIAPYGSWRSPITADRVVGSSIGLGQIALDGSDVYWNEM
                     RPAEGGRYVIQRWRDGVVAEVLPAPFSARTRAHEYGGGAFAVDGGVLYFSNDRDQLLH
                     RLKPDHPPHAITSGADKRYADGVIDRQRHRILCVCEDHGAAGEPVNTIVAIDREGRRE
                     PRVLLSGQDFYASPRLSPDGRQMVWLSWNHPNMPWDGTELWLADLGPEGMPVNPRCVA
                     GGREESVFQPLFAPDGTLYFVSDRNNWWNLYRLRQGHIEPLAPMEAEFGLPQWIFGMS
                     TYAFDSARWLVCAFNRAGAWHIAVLDTDTLRLEEIETPFSDISHVVAGNGMAVFVGAS
                     PGLPASIVRLDLATRRYEILRTSTSDVPDTGYLSAPQALAYPTTRGDTAHALYYPPVN
                     HDFSVPEHEKPPLLVLSHGGPTSATSPSLNLRTQYWTSRGFAVLDVNYRGSTGYGRDY
                     RRKLDGQWGVADVEDCIAGARYLVDRGLADEQRLAIRGGSAGGYTTLCALTFHRLFRV
                     GAVYYGVSDLGALAHDTHKFEAHYLDRLIGPYKTSRARYHERSPIHHADRLSCPVIFF
                     QGLEDKVVPPDQTEKMVNALRTRNIPVAYVPFEGEQHGFRRAENIQRALEAEFYFYSR
                     LFGFTPADPIAPVAIENL"
     gene            complement(2728..>3019)
                     /locus_tag="AC16FV_04515"
     CDS             complement(2728..>3019)
                     /locus_tag="AC16FV_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR2849362.1"
                     /translation="QHTSASLLIQENADPAVRRDLEHWFNRLAPENDPLYTHQSEGPD
                     DMPGHIKSALTATQLSIPVSGGRLALGTWQGIYLWEHRHKRGARRIVVHLGA"
BASE COUNT          636 a          953 c          894 g          536 t
ORIGIN      
        1 cagcttcacc gatgcggaaa aaaaacaggg gtatgtactg ctctgctccc acacggcggt
       61 caccgatctg gaaatcgagg caaccgagat tggcggtgcg caggatattc cggaacaaca
      121 gatcgccaca cgggtcaaga aaatagaacc gttgggcgaa gacatggcgc tgctgcattt
      181 gcaaacgccg cgcaccaagc ggctacagtt cctggccggc caggcggtga ccctcgaaac
      241 cgccaacggc gccgaggcgg attgccccgt cgccagttgc ccgtgcgacg accgcaatct
      301 ccagtttcac gtgcagcgtc tccccggcaa ccggcttgcc gactacgtgt tcaaaaaact
      361 gaaaaacggg gacatcgtcg cgctcgccgg gccgaagggg agtttcacgc tgaacgagga
      421 atcgccgcgt tccctgattt ttctcgctca cggcaccggg ttcgcgccga tcaagagcct
      481 gatcgagcac gccatggcgc gcgacgtggc cgaaagcctg catctctact ggctaagcgg
      541 cgacggaggc cattatctca acaacctctg ccgtgcctgg accgatgcgc tggataattt
      601 ccactacacg cccttggtcg cggacgcaac caaatcggcc ccggcactca agcagatcac
      661 gaaggatcac cccgatctcg gcggcttcga tatctacacc gccggccccg aggccttttt
      721 aacttcggtg aaaaaatatt tgcacgacag aaacttcccg gaaaaccagc tgattaccaa
      781 tcgtgtggat tgattttcgc tgctagagat tttcgatggc caccggcgca attgggtcgg
      841 ccggcgtaaa accgaacagt cgcgaataaa aataaaactc cgcctcaagt gcgcgctgta
      901 tattctccgc gcggcggaag ccgtgctgct cgccctcgaa cggcacgtag gccacgggta
      961 tgttgcgcgt gcgcagggcg ttcaccattt tctccgtctg atccggcggc acgaccttgt
     1021 cctccagccc ctgaaagaaa atcaccggac aggacaggcg atccgcgtga tgaatcggcg
     1081 agcgctcgtg gtaacgggca cgtgatgttt tatacgggcc gatcagacgg tcgagataat
     1141 gcgcctcgaa tttgtgcgtg tcgtgcgcca gcgccccgag atcgctcacg ccgtagtaaa
     1201 ccgccccgac gcgaaacaat cgatgaaacg tcagggcgca caacgtggta tatccgccgg
     1261 cgctgccgcc acgtatggcg agacgctgtt cgtccgccag gccgcggtcc acaagataac
     1321 gtgcgcccgc gatgcaatcc tccacgtcgg cgacacccca ttggccgtcg agcttgcgcc
     1381 ggtaatcgcg gccgtaaccc gtcgatccgc gatagttcac gtcgagcaca gcaaacccgc
     1441 ggctggtcca gtattgcgtc ctgaggttca gcgatgggga cgtggccgag gtcggaccgc
     1501 cgtggctcaa taccagcagc ggcggtttct catgctccgg cacgctgaag tcatggttca
     1561 cgggcggata atacaaagca tgtgccgtgt cgccacgcgt cgtgggatag gcgagcgcct
     1621 gcggcgcgga cagataaccg gtatcgggaa catcactggt ggaagtgcgc aggatttcat
     1681 atcggcgcgt cgccagatcg agtcgcacga tggacgccgg caagccgggt gaggcgccga
     1741 caaaaaccgc catgccattg ccggccacga catggctgat atcgctgaac ggtgtttcga
     1801 tctcttccag cctgagtgtg tcggtatcga gcacggcgat atgccacgca cccgcgcgat
     1861 tgaatgcaca caccagccac cgcgccgaat cgaaagcata agtggacatc ccgaaaatcc
     1921 attgcggcaa accaaactcc gcctccatcg gcgccaaggg ctcgatgtgt ccttgtcgca
     1981 gacgatatag attccaccaa ttattgcggt cggagacgaa gtaaagcgtc ccgtcgggtg
     2041 caaacagcgg ctgaaaaaca gattcctcgc gaccgccggc gacgcagcgc ggattgaccg
     2101 gcattccctc cggtcccagg tccgcgagcc acagctccgt gccgtcccag ggcatgttcg
     2161 gatgattcca ggaaagccat accatctgcc ggccatccgg actcaggcgc ggagaagcat
     2221 aaaaatcctg tcccgacagc aacacgcgcg gctcgcgacg gccttcgcga tcgatggcga
     2281 cgatggtatt aaccggttcc cccgcggcac cgtgatcctc gcatacacag agaatgcgat
     2341 gacgctggcg gtcaatcaca ccatcggcat agcgcttgtc cgcgcccgag gtgatggcat
     2401 gcggtggatg atccggcttg agacgatgca gaagctggtc gcggtcgttg ctgaaataca
     2461 gcacgccgcc atccacagcg aacgccccac cgccatattc atgtgcccgc gtgcgggcgc
     2521 tgaagggcgc gggcaacacc tcggccacga cgccgtcgcg ccagcgctga atgacataac
     2581 gtccgccttc cgccggacgc atttcattcc aatagacatc ggaaccgtca agggcgatct
     2641 gcccaagacc gatggagctc ccgacgacac ggtccgccgt gatcggcgat ctccaggaac
     2701 cgtacggcgc gatggtttgg cgatggttca cgcgcccagg tgtaccacga tccggcgcgc
     2761 gccacgcttg tgccggtgct cccacagata aatcccctgc caggtgccga gcgctaggcg
     2821 cccgccggaa accgggatgg aaagctgcgt cgccgtgagc gccgatttga tgtgccccgg
     2881 catgtcgtcc gggccttcgc tctggtgggt atagagcggg tcgttttccg gcgccagtcg
     2941 gttgaaccag tgttccagat cacgtctgac cgcgggatcg gcgttttcct gaatcagcag
     3001 gctcgccgag gtgtgctgc
//
LOCUS       DBNGZE010000173         5636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_95262, whole genome shotgun sequence.
ACCESSION   DBNGZE010000173 DBNGZE010000000
VERSION     DBNGZE010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_95262"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16FV_04520"
     CDS             complement(<1..201)
                     /locus_tag="AC16FV_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849363.1"
                     /translation="MPLKKELGILLGIGVAAAVIFGPLWWRPAVPCVAGDLTARAQAR
                     VPDSLKEIYLDAENMRFGLGSAA"
     gene            complement(257..1372)
                     /gene="rnd"
                     /locus_tag="AC16FV_04525"
     CDS             complement(257..1372)
                     /gene="rnd"
                     /locus_tag="AC16FV_04525"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR2849364.1"
                     /translation="MNESLIQDPTSLARSCESLRTHAWMALDTEFMRTRTYYARLCLV
                     QVATPEVIACVDTVALSGIEPLLEIIYSPHVLKGIHAARQDLEVFADIRGTPPTPVFD
                     TQIAASLCGYDDQIGYGALVESITGHKLPKLHTRADWEARPLPPDQLHYAEDDVRYLR
                     DVYQFLAQKLEELGRADWLREECVALTDPGLYRNDPRQAWRRLKQGQSLPPMAQTILR
                     ELAAWRELAAQKHNLPRGWVASDAALVETALAAPTTVEALGHIAGMGGSPARKWGEEI
                     LQAVQRGLGLKPERLWEEPQRLDRQQQALYEQLQARVRAIAAEMKISATLIAPRRELL
                     KLIASDYSGSLARGWRRALIGEELLQLCGNPPMAAAT"
     gene            1556..1948
                     /locus_tag="AC16FV_04530"
     CDS             1556..1948
                     /locus_tag="AC16FV_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849365.1"
                     /translation="MSLLAACEPLWSTPESRARDFIEALVMAPAETQPLRDIASLPPE
                     QNPETLIDDLSARVGLDFLRARQAQGVSLKFVAGETRRTDDTHRTVMVRVTYLQPGTP
                     VTGEVRFQVRIDKDDQGRWRIARVTGDN"
     gene            1969..2469
                     /locus_tag="AC16FV_04535"
     CDS             1969..2469
                     /locus_tag="AC16FV_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849366.1"
                     /translation="MPLKILIGICLLRAAPQDLPASRAWMLGALMAYAAAGVLGVLDV
                     LMLENAILATAVDTLLLAAVTHLVLQWRRLENRVTQTLTALAGCGALLSLLAWVAAGL
                     VREPFQPEWVWAAFLVWYTLVFGHILRHALSITLAAGMAASFLYLLFSISVTGLFMNP
                     VSTATN"
     gene            2501..3868
                     /gene="mpl"
                     /locus_tag="AC16FV_04540"
     CDS             2501..3868
                     /gene="mpl"
                     /locus_tag="AC16FV_04540"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR2849367.1"
                     /translation="MRIHILGIGGTFMGGVALLARDLGHDVTGSDAKVYPPMSTQLEA
                     QGIRLMEGYDAAHLDAARPDLVIIGNALSRGNALVEAVLDRGLRYTSGAQWIAENVLH
                     GRWVLAVAGTHGKTSTSSMLAWILEHAKLKPGFLIGGVPENFGVSARLGNSPFFVIEA
                     DEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAISLQFHHFVRTVPGSGLL
                     VVNAGDENLSHVLTMGCWTPVERFGSEDGWQAIARNPEASRFQVLFKKQDQGEVAWDL
                     LGSHNMANALAAIAAARHAGVPVKTAIAALAKFRNVKRRLEVRGAVRGITVYDDFAHH
                     PTAIMATLEALRARVGNARILAVLELRSNTMRMGVHRDTLAGSLVYADKVWMLRPADL
                     NWNLERVTHALDGRGQACPTVEEIIESLSREAHSDDHILIMSNGGFGNIHARLLEHLQ
                     KAPVA"
     gene            3921..4559
                     /locus_tag="AC16FV_04545"
     CDS             3921..4559
                     /locus_tag="AC16FV_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR2849368.1"
                     /translation="MKRFLGGCVMALFLLPVLAHAGFEEGRHYMVLPFPAQVETGDKI
                     EVREFFWYGCPHCYVLEPALANWLKKIPANAQFVRTPGTAPRWMIHGQAYYTFEAMGA
                     LEKLHGAFFKAMQDKPGAFNDEKAIADFAANHGVDRKKFDEAFNSFGVRLKLEKAKQL
                     NQDLNISSVPALVVDGKYMTTAAMAGGEGQMLKLLDDLIGKAAKERKKKPAK"
     gene            4712..>5636
                     /locus_tag="AC16FV_04550"
     CDS             4712..>5636
                     /locus_tag="AC16FV_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 subunit family
                     protein"
                     /protein_id="HIR2849369.1"
                     /translation="MDRKTSITTKEQSLSARWRSSTLADGNAAYLEELYEQFLRDPAS
                     LPSAWRSYFESLPRVDGVERDVSHAEIREEFRRLAHRTPSVVAPSEASQAVSVESAHK
                     QVKVLQLINAFRYRAHQTARLDPLGLREVPGIPELDPRYHGLTDLDLDAVFETGSLVG
                     PRQTTLREILAQLRATYGGTIGAEYMHITDTAEKRWLQSSIESVHGVPAYPPETRRKL
                     LERLTAAEGIEHYLHTKYVGQKRFSLEGGESLIPLLDELIQRAGANGTKEVVIGMAHR
                     GRLNVLVNILGKSPAELFREFEGRAQSNGGTG"
BASE COUNT         1038 a         1720 c         1800 g         1078 t
ORIGIN      
        1 ggcggcggaa cccaggccga accgcatgtt ttcggcgtcc agataaatct ccttcagtga
       61 atcgggcacc cgcgcctgcg cgcgcgccgt caggtcgccg gccacgcagg gaacggccgg
      121 gcgccaccac agcggtccga aaatgaccgc ggccgcgacg ccgatgccga gcaggatgcc
      181 gagttctttt ttcagcggca tggcaacaga taatgacagg acgaacgaag cgtagcggag
      241 taggacttca ttttattcag gtagccgctg ccattggtgg gttgccacag agctgcaaca
      301 gttcttcgcc gatcagcgcg cgtcgccagc cgcgcgccag gctgccggag tagtcgctgg
      361 cgatgagttt cagcagttcg cggcgtggcg ctatcagcgt cgcgctgatt ttcatttccg
      421 cggcgatggc gcgcacgcgt gcctgcagtt gttcgtacag tgcctgctgc tgccggtcga
      481 gacgctgcgg ttcttcccac aggcgttcgg gcttcaggcc caggccgcgc tggacagcct
      541 gtaaaatttc ctcgccccac ttgcgcgccg gcgatccacc catgccggcg atgtggccga
      601 gtgcctccac ggtcgttgga gcagcgagag cggtttccac cagtgcggca tcggaagcga
      661 cccagccgcg cggcaggttg tgtttctgcg ccgcgagttc gcgccaggcc gccagctcgc
      721 gcaatattgt ctgcgccatg ggcggcaagg attgtccctg cttcaggcga cgccaggcct
      781 ggcgcgggtc gttgcggtaa agcccgggat cggtgagcgc gacacactcc tcgcgcagcc
      841 agtcggcgcg tccgagctcc tcgagtttct gcgcgaggaa ctgatacacg tcgcgcaggt
      901 aacgcacatc atcctcggcg taatgcaact gatccggcgg gagcgggcgg gcttcccagt
      961 cggcgcgggt atgcagcttg ggcagcttgt gaccggtgat ggattccacc agcgcgccgt
     1021 agccgatctg gtcgtcgtag ccgcacagcg aggcggcgat ctgggtgtcg aacaccggcg
     1081 tgggcggggt tccgcggata tcggcgaaca cctcgagatc ctgacgcgcg gcgtggatac
     1141 ccttgagcac gtgtggcgaa taaattatct ccagcagcgg ctcgatgccg gacaaagcga
     1201 ccgtatcgac acaggcgatg acctccgggg tcgccacctg taccaggcac aggcgtgcgt
     1261 aataggtgcg cgtgcgcatg aattccgtgt ccagcgccat ccacgcatga gtgcgtaatg
     1321 attcgcacga acgtgcaagc gacgtcgggt cctggatcag cgattcgttc atgcatgcaa
     1381 tccttgttct cgcagtgttc ttcctgtcat gagtattatt ggaagtgtat gcatttcccg
     1441 cgctcggccc aactgcgggt cagggcgatc gattatggtt tatcattagg cgatgaaccg
     1501 gtgcgcgcaa gagacgggta aacggaaaat aacatggacc gccggaatat ggctggtgtc
     1561 gcttctggcc gcttgcgagc cattgtggag caccccggaa agccgcgccc gggatttcat
     1621 cgaggccctg gtgatggccc cggcagagac ccagccgctg cgcgacatcg ccagtcttcc
     1681 gccggaacag aatcctgaaa cgctgatcga tgacctctcc gcccgcgttg gtctggattt
     1741 tctgcgcgcc cggcaggccc agggcgtgtc gctgaagttt gtggcgggcg aaacccggcg
     1801 gacggacgac acgcaccgca cggtcatggt ccgcgtcacc tatctgcagc ccggaacgcc
     1861 cgtgaccggt gaagtccggt ttcaggtccg gatcgataag gacgatcagg gccgctggcg
     1921 catcgcccgt gtcacgggcg ataactgaaa cacctgcatg tattaaacat gccgctgaaa
     1981 atattgatcg gtatttgcct gttgcgtgcc gcgccccagg acctgccggc atcgcgcgcc
     2041 tggatgctgg gggcgctcat ggcctacgca gcggcgggtg tgctcggcgt gctcgatgtt
     2101 ctcatgcttg aaaacgccat cctggccacc gcggtggaca cgctgttgct cgccgccgtg
     2161 acgcatctgg tattgcagtg gcggcggctg gaaaatcgcg tgacccagac gctcaccgcg
     2221 ctggccggtt gtggcgccct gctgtcgttg ctggcctggg tcgccgccgg tcttgtccgt
     2281 gaaccgtttc agccggaatg ggtatgggcg gccttcctgg tgtggtatac gctggtattc
     2341 gggcacattt tgcgacacgc cctgtctatt accctggcgg ccggcatggc cgcgagtttt
     2401 ttgtatctgc ttttttcgat aagcgtaacg ggcctgttca tgaatccggt ttcaacggcg
     2461 acgaactgat aatgacagga cgagaaacag gattttattg atgcgcatcc atattctcgg
     2521 tattggcggg acattcatgg gcggtgtggc gctgctcgcg cgcgatctcg gtcacgacgt
     2581 gaccggctcg gatgccaagg tgtatccgcc catgagcacg cagctggagg cgcagggcat
     2641 ccggctgatg gagggttacg acgcggcgca cctcgacgct gcccggcccg atctcgtgat
     2701 catcggcaat gcgctgtcgc gcggcaatgc gctggtcgag gcggtgctcg accgcggcct
     2761 acgctacacc tccggcgcgc aatggatcgc ggaaaatgtg ttacatggcc gctgggtgct
     2821 cgcggtcgcg ggcacgcacg gcaagaccag cacgtccagc atgctcgcct ggatcctgga
     2881 gcacgccaaa ctcaagcccg gttttttgat cgggggcgtg ccggagaact tcggtgtttc
     2941 ggcgcgtctc ggcaatagtc ctttttttgt catcgaggcc gacgaatacg acaccgcctt
     3001 tttcgacaag cgctcaaagt tcgtccatta ccgcccgcgc accgccatcc tgaacaacct
     3061 ggaattcgat cacgcggaca tctttcccga cctcgccgcg atctcgctcc agtttcatca
     3121 cttcgtgcgc accgtcccgg gatcgggact gctggtggtg aacgccggcg atgagaacct
     3181 gtcccatgtg ctgacgatgg gctgctggac ccccgtcgaa cgtttcggca gcgaggacgg
     3241 gtggcaggcc atcgcacgca accccgaggc cagccggttt caggtgttgt ttaaaaaaca
     3301 ggatcagggc gaggtggcgt gggatttact cggcagtcac aacatggcca atgccctggc
     3361 cgcgatcgcg gccgcgcgtc acgccggggt gccggtcaag accgccatcg cggcgctggc
     3421 gaaatttcgc aacgtcaagc gccggctcga ggtgcgcggc gcggtgcgcg gtattacggt
     3481 atatgatgat ttcgcgcatc atcccaccgc gatcatggcc acgctcgagg ccctgcgcgc
     3541 gcgcgtcggc aacgcgcgca tcctggcggt gctggaactg cgctccaaca ccatgcgcat
     3601 gggcgtgcac cgcgacacgc tggccggctc gctggtctat gccgacaagg tgtggatgct
     3661 ccggcccgcc gacctcaact ggaacctgga acgggtgacg catgccctgg acgggcgcgg
     3721 acaggcatgc ccgacagtag aggagattat cgagtcgttg tcccgcgagg cgcactcaga
     3781 cgatcatata ttgatcatga gcaacggcgg tttcggcaac atccacgccc gcttgctcga
     3841 gcatttacag aaggccccgg ttgcatagct tgcccggttt ttttacgctc aattaacaaa
     3901 tgtctttcgg agaaccataa atgaagcgat ttttaggcgg ctgtgtgatg gccctgtttt
     3961 tgcttccggt gctggcccat gccggcttcg aggaaggacg gcactacatg gtcctgccct
     4021 ttccggcaca ggtcgagacc ggcgacaaga tcgaggtgcg ggagtttttc tggtacggct
     4081 gcccgcattg ctatgtgctg gaaccggcgc tggcgaactg gctgaagaaa attcccgcga
     4141 acgcacaatt tgtgcgcaca cccggcactg ccccgcgctg gatgatccac ggtcaggcct
     4201 attacacctt cgaggcaatg ggcgcgctgg aaaaactgca tggcgctttt ttcaaggcga
     4261 tgcaggataa gccgggtgcg ttcaatgatg aaaaggccat cgctgatttt gccgcgaacc
     4321 acggcgtgga tcgcaagaaa ttcgacgagg ccttcaattc cttcggcgtg cgtctgaagc
     4381 tggagaaggc caagcagctc aaccaggacc tgaacatcag ttccgtgccc gcgctcgtgg
     4441 tggatggcaa gtatatgacc acggccgcca tggccggcgg cgagggacag atgctgaagc
     4501 tgctggatga cctgattggc aaggccgcca aggaacgcaa gaaaaaaccc gcaaaatagt
     4561 gatggcttgt gacgccgggg acgcccggcg gcttcaagtc gtgtcgcgtg cttcgcgtcc
     4621 cgcggcgcgc agtcgggagg cacagacctg ctaagctagt gttagcggtg caccgccaca
     4681 ttccggcatt ccggcgattt cacgctgtga catggacaga aagacgagca tcaccaccaa
     4741 ggagcaaagc ctgagcgccc gctggcgctc ctcgacgctg gcggacggca atgccgcgta
     4801 cctcgaggag ctctatgagc agtttttgcg cgatcccgcc tccctgcctt ccgcctggcg
     4861 cagttatttc gagagcctgc cgcgcgtcga tggcgtggag cgggatgttt ctcatgccga
     4921 gatccgcgag gaattccgcc ggcttgcgca tcgaacgccc tccgttgtcg caccctcgga
     4981 agccagtcag gcggtgtctg tcgaatccgc gcacaagcag gtcaaggtgt tgcaactgat
     5041 caacgctttc cgttatcgcg cgcatcagac ggcgcggctc gatccgttgg ggctgcggga
     5101 agtgccgggc atccccgagc tcgacccgcg ttaccacgga ctcaccgacc tcgatctcga
     5161 cgcggtattc gaaaccggat cgctcgtggg gccgcgtcag accacgctgc gcgagattct
     5221 ggcgcagttg cgcgccacct acggcggcac catcggtgcc gagtacatgc acatcaccga
     5281 cacggcagaa aagcgctggc tgcaaagcag catcgagagc gtacacggcg tgccggccta
     5341 tccacccgag acgcggcgca agttgctcga gcggctgacc gcggccgagg gcatcgagca
     5401 ctacctgcac accaaatacg tggggcagaa gcgcttctca ctggaaggcg gcgaaagcct
     5461 gattcccctg ctggacgagc tgatccagcg cgccggcgcc aatggaacaa aagaagtggt
     5521 catcggcatg gcgcaccgcg ggcggctcaa cgtgctggtc aacatcctcg gcaaaagccc
     5581 ggccgaattg ttccgcgagt tcgagggaag agcgcaatcc aacggcggca ccggcg
//
LOCUS       DBNGZE010000174         7515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_442312, whole genome shotgun sequence.
ACCESSION   DBNGZE010000174 DBNGZE010000000
VERSION     DBNGZE010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_442312"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC16FV_04555"
     CDS             complement(<1..272)
                     /locus_tag="AC16FV_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849370.1"
                     /translation="MIALGIAAPMLFLAALILSSRLHQNTPPLVHVILAAGVMPLIMA
                     AMIYFAPVLSHSRAPSWPVLLLPGVALVAGVGAAASVFWWRDLIFAP"
     gene            complement(330..470)
                     /locus_tag="AC16FV_04560"
     CDS             complement(330..470)
                     /locus_tag="AC16FV_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849371.1"
                     /translation="MKKSRWILIVTFVIVVWMALMVGASYFGYEYKSPVRIENWKPEP
                     AP"
     gene            complement(489..2171)
                     /gene="ilvD"
                     /locus_tag="AC16FV_04565"
     CDS             complement(489..2171)
                     /gene="ilvD"
                     /locus_tag="AC16FV_04565"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR2849372.1"
                     /translation="MASKLNRYSSRITQPKSQGASQAMLYGTGLTRTDMDKAQVGIAA
                     NWWEGNTCNMHLNDLAARVKEGVVAAGLVGMRFNTIGVSDGISMGTDGMSYSLQSREI
                     IADSIETVVGAQWYDALITIPGCDKNMPGCLIAMGRLNRPSLMIYGGTIKPGHSKKGE
                     TLDIVSAFQSYGAFIAGKIDEEAREDIVEHSCPGAGACGGMYTANTMASAIEALGMSL
                     PYSSSVPAVEPGKWQECLDAGKAIRHLLEKDIKPRDIMTRAAFENAMVIVMALGGSTN
                     AVLHLIAMARAVGVPLTIDDFQKVSDRVPFLADLKPSGKYVMEDLHNVGGTPAVMKYL
                     LENGLLQGDCLTVTGKTVAQNLSPLPGLKPGQDVFMPLEKPIKPSGHIRILRGNLAPD
                     GAVAKITGKEGLRFSGPARVYDCEEDMLKGLERSEIKKGEVVVIRYEGPKGGPGMPEM
                     LTPTSAIMGAGLGKDVALMTDGRFSGGSHGFIIGHVVPEAQEGGPIALLRDGDVITID
                     AGKNELSVAVSDAEMAKRKAAWKMPPYKATQGTLYKFIKNVKNASEGCVTDE"
     gene            complement(2304..2708)
                     /locus_tag="AC16FV_04570"
     CDS             complement(2304..2708)
                     /locus_tag="AC16FV_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849373.1"
                     /translation="MRAQKVSYFAMQVPNRAGEAGRILAGLAKAGINLLAFTGFPSGR
                     QAQMDFIPVNPKTFLAVARGMKLRLRERKTAFLIRGSDQRGAIARITARLATAKINVT
                     AVDAVSAGGGRFGAILWVKQKDVNRTARALGA"
     gene            3020..3694
                     /locus_tag="AC16FV_04575"
     CDS             3020..3694
                     /locus_tag="AC16FV_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HIR2849374.1"
                     /translation="MAEKPKIEPFAVKDCALSAIATGRRAQNLRELRDGLLTVHPGSI
                     YYHFWGRLLRPGFDDPEFNNDFASWVRHRLHDVKLAERLGVIDPADFSEIEALRQEII
                     DVIEERLDETEFLSWAPRDRQFHFTRAQTVVFDTRRRLEHPRELAATVPGLSVGSIYY
                     HAIDARRREPHGMDDFRAWLARFGDRYADLSTRLAGLDPYFVTLTELRDQLSFVFSDY
                     FKEDGR"
     gene            3691..4929
                     /locus_tag="AC16FV_04580"
     CDS             3691..4929
                     /locus_tag="AC16FV_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2849375.1"
                     /translation="MTMLSDYAGVVGEDVIDHLKQLAATLKGMKVVHVNSTRQGGGVA
                     EILSWLIPLQCELGMEARWEVISGKNAFYQCTKHFHNALQGNPALIAESLLKAYEETN
                     RNAAEELGPVLRDADMVFIHDPQPAALLRHFPARKGKWIWRCHIDASHPYRPVWKYLR
                     GLVAGYDASIFSLADFVQMLPHPVYLIPPSIDPLSEKNMELGHEEIQGAYARFGIDPA
                     RPMMLQVSRFDRFKDPIGVIEAYRLATAFIPALQLVLAGGGASDDPEGEAVLAEVRAA
                     AREDPDIHILLLPPDAHRTINALQRAADFVLQKSLKEGFGLTVAESMWKGKPVIGGDT
                     GGIRLQVINHHTGFLVNSPEGAALRIRYLLHHREKLTEMGGKAREFVRQNFLTTRQLR
                     EYLTLMVGLMHGAEDRVRLG"
     gene            4937..5734
                     /gene="otsB"
                     /locus_tag="AC16FV_04585"
     CDS             4937..5734
                     /gene="otsB"
                     /locus_tag="AC16FV_04585"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR2849376.1"
                     /translation="MQTADQQRHLADFFKRLATAPHRALLLDYDGTLAPFKIRRDEAL
                     PYPGVREILDAIMESGHTRLVIVSGRWTRDLIPLLGLRRLPEVWGSHGWEQLKPGGEY
                     TVARMDEAGLRHLADADTWTAEIEAQGGHCEIKPGGLGIHWRGLDQERIADIRSLVFQ
                     NLLMKEMHKTLVWHDFDGGIELRVPGRNKGFVVDTVLSDMGGETAAAYLGDDTTDEDA
                     FKAIHGRGIGVLVRPQFRTTAADFWLRPPAELLDFLKQWHETCGGKP"
     gene            5731..6798
                     /locus_tag="AC16FV_04590"
     CDS             5731..6798
                     /locus_tag="AC16FV_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR2849377.1"
                     /translation="MNLFERTFYGNPLYDWLAAAAIGIMLALSFHVAQRVMLRRLASM
                     SRRTETRIDDFIVDILDHTRFLFLLVIAFYFGSQYLELPPKTARLIAHIAIVILLVQV
                     ALWGNRGIALWLGDYLKRHRDTDAAGATTMSVLGFIARVALWSVLLLMILDNLGFNIT
                     ALVASLGIGGVAVALAVQNILGDIFASLSIAMDKPFAIGDFIIVDDAMGSVEYIGLKT
                     TRLRSLSGEQIVFSNNDLLKSRIRNYKRMYERRVVFGFGVTYQTPLSKLEAIPPKVKE
                     IVTAQQKTRFDRAHFKEYGDSSLNFEVVYFVQDPDFNLYMDIQQAINLALMRYFDQER
                     IDFAYPTRTLYLQRETGGVHG"
     gene            6791..>7515
                     /locus_tag="AC16FV_04595"
     CDS             6791..>7515
                     /locus_tag="AC16FV_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849378.1"
                     /translation="MASPTRPRITRSLRLGLLIAAIIGLTTGLFAWHQVQSERRNDIE
                     EVARRAHALAQQLSDRVLYALTLPDIQARALLQPRIEGYQRLLGYAVYRADGRLVAAG
                     KTISDFTNKLDGAMREALQSGNEVTQLTRGAGMRVHVFAKKFPEKGEAEGVLVVVHDV
                     SYIDDRALARLAQFAFWVLIVSLLAVALVVGSTWFVYERPLTRLADWMRRLRVENVAE
                     SPPPGLPSGRLRSESDRLAASFRA"
BASE COUNT         1405 a         2389 c         2288 g         1433 t
ORIGIN      
        1 ggcgcaaaaa tcagatcgcg ccaccagaat acactggctg ccgccccaac accggcaacc
       61 agggccacgc cgggaagaag cagcaccggc caagaaggcg cgcggctatg cgacagcacc
      121 ggcgcgaaat agatcatcgc ggccatgatg agcggcataa caccggccgc caggatcacg
      181 tgcacaagcg gtggggtgtt ttgatgcagg cgggaagaca gaatcagcgc ggcaagaaag
      241 agcatgggcg ccgcgatgcc cagcgcgatc atgacgcgac gcgcgggttg cgaggcatca
      301 cgcatgcctg gcgtccattc tggcgcgtgt cacggagctg gttccggttt ccagttttcg
      361 atgcgtaccg gtgacttgta ctcgtagccg aaatagctgg cgcccaccat gagcgccatc
      421 cagacgacaa taacgaacgt gacaatcaga atccagcggc tttttttcat ggcgccggtt
      481 gcttgctctc actcgtccgt gacacaacct tccgaggcgt ttttgacgtt cttgatgaac
      541 ttgtagagtg ttccttgcgt ggccttgtag ggcggcattt tccaagccgc cttgcgcttg
      601 gccatttccg cgtcgctcac cgccacgctg agttcatttt tcccggcgtc gatggtgatg
      661 acgtcgccgt cgcgcaacag cgcgatcggt ccgccttcct gcgcctccgg cacgacatgg
      721 ccgatgatga aaccgtgcga gccgccggag aagcgcccgt cggtcatgag cgccacgtcc
      781 ttgcccaggc ccgcgcccat gatggccgag gtcggcgtca gcatctcggg catgccggga
      841 ccgcccttcg ggccctcgta gcggatcacc accacttcgc cctttttgat ttccgaacgc
      901 tcgaggcctt tcagcatatc ctcctcgcag tcatacaccc gcgccgggcc ggaaaaacgc
      961 aggccttcct tgccggtgat cttggccacg gccccgtccg gcgcgagatt gccgcgcagg
     1021 atccggatat gtccgctcgg cttgatcggt ttttccagag gcatgaacac gtcctggccc
     1081 ggcttcaggc ccggtagcgg tgacagattt tgcgcgacgg ttttgcccgt caccgtgagg
     1141 caatcgccct gcagcaatcc attttcgagc aagtacttca tcactgccgg cgtgccacca
     1201 acgttgtgaa gatcttccat gacatacttg ccgctcggtt tgagatcggc gaggaagggt
     1261 acgcgatcgg acaccttctg gaaatcgtcg atcgtcagcg gcacacctac cgcgcgcgcc
     1321 atggcaatga ggtgcaacac ggcgttggtg gaaccgccga gcgccatgac gatgaccatg
     1381 gcgttctcga acgccgcgcg cgtcatgatg tcgcgtggct tgatgtcttt ctcgagcagg
     1441 tggcggatag ccttgccggc atcgaggcac tcctgccact tgcccggctc gactgccggc
     1501 accgacgagc tatacggcaa cgacatgccg agcgcctcga tggccgaggc catggtattg
     1561 gccgtgtaca tgccaccgca ggcaccggcg ccgggacagg aatgctcgac aatgtcctcg
     1621 cgcgcttctt cgtcaatctt gccggcgatg aacgcaccgt agctctgaaa cgcggagacg
     1681 atatccagcg tctcgccctt cttgctgtga ccgggcttga tggtgccgcc gtagatcatg
     1741 agcgaggggc ggttgagccg gcccatggca atgagacatc cgggcatgtt tttgtcgcag
     1801 cccggaatgg tgatgagcgc gtcgtaccac tgcgcaccca cgaccgtctc gatcgaatcg
     1861 gcgatgatct cgcgcgactg caacgaatac gacatgccgt cggtgcccat ggaaatgccg
     1921 tcagacacac cgatggtgtt gaagcgcatg cccaccagcc ccgcggccac cacgccctcc
     1981 ttcaccctgg cggcgaggtc gttcagatgc atgttgcagg tgttcccctc ccaccagttg
     2041 gcggcaatgc cgacctgggc cttgtccatg tccgtgcgcg tcagaccggt gccgtagagc
     2101 atggcctgcg acgcgccctg cgacttgggt tgcgtgatgc gggaactgta acgattcagt
     2161 ttgcttgcca tgaaaactcc gcccgtgtcc ggtgaccgac cgatgaaagc cggtcattct
     2221 agcgcagacc ggggagttcg ctgaagcgga aaacaggcga tacggatgct ccgccgcgcg
     2281 cggcggagcg gcatgctggc gtgtcaggcc ccgagtgcgc gcgccgtgcg gttcacgtcc
     2341 ttttgcttga cccagagaat cgcgccgaag cggccgccac cggcactgac cgcgtccacg
     2401 gccgtgacgt tgatcttggc cgtggccagt cttgccgtaa tcctggcgat ggcgccgcgc
     2461 tgatccgaac cgcggatgag aaacgcggtc ttgcgttcgc gcagccgcag tttcatcccg
     2521 cgcgcgacag caagaaatgt ttttggattg acgggaatga agtccatctg cgcctgccgt
     2581 ccactgggga aaccggtgaa cgcgagcaga ttgattccgg ccttcgccaa ccccgccagg
     2641 atccgtcccg cctcgcccgc gcgattcgga acttgcattg cgaaataact gactttttgt
     2701 gctctcatgg ttttctcacc tccgtgcgct gggaattgaa cgtccgtcgg tttttcataa
     2761 cacgccatcg gcggaaatac aaacatgcca ttcgacggtt ggcacgggcg catcaataaa
     2821 ggtaatattt gccgggacaa tcagattttc cttccgctgt cccgcccgcg ccaccgcaac
     2881 gaccttggcc gggccgggtc agccgctgta atcaagctgt tccggcgaag cggtatcgcg
     2941 ccaacaaaaa tgtaggttcc gcctccatgg gtggcggttc tcaagccgag tctgacaccg
     3001 gtattttttc gaggtgaaga tggctgaaaa accgaagatc gaaccgtttg ccgtcaagga
     3061 ttgcgccctg tccgcgatcg ccaccggccg gcgcgcgcaa aacctgcgcg agttgcgcga
     3121 cggcctgctc acggtgcacc cggggagtat ttattaccac ttctggggca gacttctgcg
     3181 accgggcttc gacgatcccg aatttaacaa cgacttcgcc tcctgggtgc gacaccggtt
     3241 gcatgacgtc aagctcgctg aacgcctcgg ggtcattgat cctgccgatt tcagtgaaat
     3301 agaagcgctg cgccaggaga tcatcgacgt catcgaggag cgtctcgatg agactgaatt
     3361 cctctcgtgg gcgccacgcg accgccaatt ccacttcacc cgcgcccaga ccgtggtgtt
     3421 cgacacccgg cgacgtctcg agcatccccg ggaactggcc gcgacggttc ctggcctgtc
     3481 tgtgggcagt atctattatc acgcgatcga cgcgcgccgc cgcgaacccc acggcatgga
     3541 cgatttccgc gcctggctcg cgcgcttcgg cgaccgctat gccgacctga gcacccgact
     3601 ggcggggctc gacccctact tcgtgacgct gaccgaattg cgcgatcagt tgtctttcgt
     3661 tttctcggac tatttcaagg aggacggccg atgacgatgt tatccgatta cgccggggtc
     3721 gtgggagagg acgtcatcga tcacctcaaa caactggccg cgacgctcaa gggcatgaag
     3781 gtcgtgcacg tcaattccac gcgtcagggt ggcggcgtgg ccgaaatcct gagctggctg
     3841 atcccgctcc agtgtgaact gggcatggag gcgcgctggg aagtcatcag cggcaaaaac
     3901 gcgttctacc agtgcaccaa gcattttcat aacgccttgc agggcaatcc cgctttaatc
     3961 gccgaaagcc tgctgaaggc gtacgaagag accaaccgca acgccgcgga agaacttggc
     4021 ccggtgctgc gcgacgccga catggttttc atccacgatc cccagccggc cgcactgctc
     4081 aggcacttcc cggcccgcaa gggcaagtgg atctggcgct gtcatatcga cgccagccac
     4141 ccctaccggc cggtgtggaa gtatctgcgc ggcctcgtgg ccggttacga cgccagcata
     4201 ttctcgctcg ccgatttcgt gcagatgctg ccgcacccgg tgtacctgat cccgcccagc
     4261 atcgatccat tgagcgaaaa aaacatggaa ttgggacacg aggagataca gggcgcatac
     4321 gcgcgcttcg gcattgatcc cgcacggccg atgatgctgc aagtatcgcg cttcgatcgc
     4381 ttcaaggacc cgatcggcgt gatcgaggcc tatcgcctgg cgacggcgtt cattcccgca
     4441 ttgcaactgg tactggccgg cggcggcgcc agcgacgatc ccgagggcga ggcggtgctg
     4501 gccgaggtgc gcgccgccgc ccgggaggac cccgatatcc atatcctgct gctgccaccc
     4561 gacgcgcatc gcaccatcaa cgcactgcag cgcgccgcgg acttcgtgct gcagaaatcg
     4621 ctcaaggagg gattcggcct gaccgtcgcc gagtcgatgt ggaaaggcaa acccgtgatc
     4681 ggcggcgaca ccggcggtat ccgcctgcag gtgatcaatc accataccgg cttcctggtg
     4741 aactcacccg agggcgcggc cctgcgcatc cgttacctgc tgcatcaccg cgaaaaactc
     4801 acggagatgg gtggcaaggc gcgcgagttc gtgcgccaga atttcctgac cacgcgccag
     4861 ttgcgcgaat atctgacgtt gatggtcggc ctgatgcacg gggccgaaga ccgggtcagg
     4921 ctgggataag cacgccatgc aaaccgccga ccaacagcgt catctcgcgg attttttcaa
     4981 gcgcctggcc acggcgccgc accgggcgtt gctgctcgat tacgacggca cgctcgcgcc
     5041 gttcaaaatc cggcgcgatg aggcgctgcc ctatcccggc gtgcgcgaga tcctcgacgc
     5101 catcatggaa agtggtcata cccggctcgt catcgtcagc ggccgctgga cccgtgatct
     5161 cattccgctg ctcggcctca ggcgtctgcc cgaagtctgg ggctcgcacg gctgggaaca
     5221 gctcaagccc ggcggtgaat acaccgtcgc gcgcatggat gaagcggggc tgcgacatct
     5281 cgcggacgcc gacacctgga ccgccgagat tgaggcacaa ggaggacatt gcgagatcaa
     5341 gcccggcggt ctgggcatcc actggcgcgg ccttgaccag gaaaggatcg ccgatatccg
     5401 cagcctcgta ttccagaacc tgttgatgaa ggaaatgcac aaaacccttg tatggcatga
     5461 cttcgacggc ggcatcgaac tgcgcgtgcc cggccgtaac aagggattcg tcgtggacac
     5521 cgtgctttcc gatatgggcg gggaaaccgc cgcggcctat ctcggtgacg acaccaccga
     5581 tgaagacgca ttcaaggcca tccacggccg cggcatcggc gtgctggtac gcccgcaatt
     5641 ccggaccacg gcggcggatt tctggctgcg gcccccggcg gaactgctcg actttctgaa
     5701 acagtggcac gagacctgcg gaggaaagcc atgaacctgt tcgagcgaac cttctacggc
     5761 aacccccttt acgactggct cgcggcagct gcgataggca tcatgctggc gctgtcattc
     5821 catgtcgccc aacgcgtcat gctccgccgc ctcgcctcga tgtcacgcag aacggaaacg
     5881 cgcatcgacg attttatcgt ggatattctg gaccataccc gcttcctgtt cctcctggtc
     5941 attgccttct atttcggatc gcagtatctg gagctgccgc cgaaaacggc gcgcctgatc
     6001 gcgcacatcg ccatcgtcat cctgctggtg caggtggcgt tgtggggcaa ccgcgggatc
     6061 gcgctgtggc tcggcgacta cctgaaacgg cacagggaca ccgacgccgc cggcgccacc
     6121 accatgtcgg tgctgggctt catcgcgcgg gtggcgttgt ggtcggtact gctgctgatg
     6181 atactggaca acctcggatt caatatcacc gcgctcgtcg caagcctcgg catcggcggc
     6241 gtcgccgtcg ccctcgcggt acagaacata ctgggcgata ttttcgcctc gctgtccatc
     6301 gccatggaca agcccttcgc gatcggcgac ttcatcatcg tggatgacgc gatgggcagc
     6361 gtcgaataca tcggcctcaa gaccacccgc ctgcgcagcc tgagcggcga gcagattgtt
     6421 ttttccaaca acgacctgct gaagagccgg atacgcaact acaagcgcat gtacgagcgg
     6481 cgcgtggtgt tcggcttcgg cgttacctac cagacgccgc tctcgaagct ggaggccatt
     6541 ccgccgaagg tgaaagaaat agtcacggca cagcaaaaaa cccgcttcga ccgcgcgcac
     6601 ttcaaggaat atggagactc gtcgctgaac tttgaggtcg tctatttcgt gcaggacccg
     6661 gatttcaacc tgtacatgga catccagcag gcgatcaacc tggcgctcat gcggtatttt
     6721 gatcaggaac gcatcgattt cgcgtatccc acgcgcacgc tgtacctgca gcgcgagacc
     6781 ggaggcgttc atggctagcc cgacccgtcc gcgcataacg cgttcactgc gcctcggtct
     6841 gctgatcgcc gccatcatcg gactgaccac cggcctgttc gcctggcacc aggtgcaaag
     6901 cgagcgtcgg aacgacatcg aggaggtggc gcggcgtgca catgcgttgg cgcagcaact
     6961 ttccgaccgg gtgttgtacg cgctgacgct gccggacata caggcgcgcg cgttgttgca
     7021 gccgcggatc gagggttatc agcgactgct cggctacgcg gtgtatcgtg ccgatggccg
     7081 gctggtcgcg gcaggcaaga ccatctccga cttcaccaac aagcttgacg gcgcgatgcg
     7141 tgaggcgctg caatccggaa atgaggtcac gcagctcacg cgcggcgcgg gcatgcgggt
     7201 gcatgtattt gccaagaagt ttcccgaaaa gggcgaggct gagggcgttc tggtggtggt
     7261 gcacgacgtg tcctatatcg atgaccgcgc tctcgcccgg cttgcgcagt tcgccttctg
     7321 ggttctgatc gtctcgctgc tcgcggtagc gctggtcgtg ggcagcacct ggttcgtgta
     7381 cgagcgaccg ctcaccaggc tcgccgactg gatgcggcgc ctgcgggtgg agaacgtcgc
     7441 cgaatcgccg ccgccggggc tgccctcggg acggctgcgc agcgaatcgg accggctggc
     7501 ggcgtcgttc cgcgc
//
LOCUS       DBNGZE010000175        11168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1206577, whole genome shotgun sequence.
ACCESSION   DBNGZE010000175 DBNGZE010000000
VERSION     DBNGZE010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1206577"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC16FV_04600"
     CDS             <1..322
                     /locus_tag="AC16FV_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849379.1"
                     /translation="THECNRLSRLMFNTTRSKPRFIAWLLSAMLLWVGAVAAAHELDH
                     ALDHHHELCALHHYAGHDGGLPSSGAHPPVPPVETRLDLPTLHQSISKSRPAPYAVRA
                     PPQV"
     gene            458..1717
                     /locus_tag="AC16FV_04605"
     CDS             458..1717
                     /locus_tag="AC16FV_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849380.1"
                     /translation="MYKPIFMASLLFAAHAVHAAEPPVTPQQLHDLEQRVRELEAANV
                     AAGKAEARQTSNNFNPAISLILDGKLSGFSRDPATYALPGFALGEGVGPGDEGLRLGE
                     SELVMSSNIDDKFYGQFTAALTPENETEIEEAFIETLALGGGATIKAGRFFSHIGYLN
                     PVHAHAWDFADQPLVYRAMLGNQYGDDGAQLRWVAPTDVFLELGAEIFRGDSFPAGGA
                     ANSGNGTRTVFAHIGGDIGADHAWRVGFSRLDADAVNRATGTVATPDLFTGNSTVTGV
                     DLVWKWAPNGNPKQRNFKFQTEYFKRDENGAFDPASSGSPLPYNGKQTGWYAQTVYQF
                     MPRWRVGLRQDRLHVDTANAALAGIVLDNTGHEPKRTSAMTDFSNSEFSRLRLQFNRD
                     ESRAMTKDNQWYLQYVMSLGAHGAHGF"
     gene            1734..2636
                     /locus_tag="AC16FV_04610"
     CDS             1734..2636
                     /locus_tag="AC16FV_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR2849381.1"
                     /translation="MKIFHQLLAGLVFLIAFPAMAEIKVFACEPEWSALVQELGGDKV
                     TVFTATTALQDPHHIEARPSLIARLRQADLLVCTGAELEAGWLPALLRQAGNAAVQPG
                     KAGYFEAANYVRKLEIPARLDRAEGDVHAAGNPHIQTDPRNIGAVATALARCLAEIDP
                     AGANLYQTRQRNFAQRWDVAIKRWEQQAAPLKGVHIVVQHKGFPYLENWLGLRQVAAL
                     EPKPGVEPTSAHLTEVLEQLQRQPAKMILRAAYSDDRSSEWLAGRAGIPAVVLPFTVG
                     GSDKAKDLFGLFDDTVQQLLGALK"
     gene            2633..3415
                     /locus_tag="AC16FV_04615"
     CDS             2633..3415
                     /locus_tag="AC16FV_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR2849382.1"
                     /translation="MNLDALDISILGPAFCAGLLVLATHVPMGQQVLARGIVFIDLAI
                     AQIAGLGVIAADGMGWESQGFAVQIAAVGAALLGALLLTFTEKRWPEVQEALIGVLFV
                     LAACAGILLLAGNPHGGEHLKDLLVGQILWVSYGQLLPVLPLTVGILALWFGARDRLG
                     RIGFYAMFALAVTASVQLVGIYLVFASLILPALATYRLRTAQRLTWGYGAGAGGYAIG
                     IMASALFDLPTGAVAVWTLALVAFATALLVPRLTKRREVAGT"
     gene            complement(3444..4064)
                     /locus_tag="AC16FV_04620"
     CDS             complement(3444..4064)
                     /locus_tag="AC16FV_04620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2849383.1"
                     /translation="MSIHHEKSAAPPSASVSGATDVHHDAIKIRIRKIYWADWRRLTE
                     MFREIFPELTAQQISHYILNYEDTIAVAEAREGIVGFYHFIPRKDEGTAWLNYLGVVS
                     SCHRGGVGTQLLRDYEERAAQMGLRWAEFDVLQHNQRAIKFYEKHGYTRLHPVDNKFR
                     YRKALAAGVQAVADSRPIRIRPRLERWGRRMLYLVLAGLSLWFASH"
     gene            complement(4086..4532)
                     /gene="fur"
                     /locus_tag="AC16FV_04625"
     CDS             complement(4086..4532)
                     /gene="fur"
                     /locus_tag="AC16FV_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR2849384.1"
                     /translation="MRNVNTGGAELKKAGLKATLPRLKILGILEAAGHPHMTAEEVYK
                     ELLNMGEEVGLATVYRVLTQFEAAGLVMRHNFEGGRSVFEINQGGHHDHMVCLECGKV
                     FEFYDTAIEERQHKIAEKAGFHIDDHSLYLYGVCDGMKKHGKCSKR"
     gene            4640..4978
                     /locus_tag="AC16FV_04630"
     CDS             4640..4978
                     /locus_tag="AC16FV_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR2849385.1"
                     /translation="MRHWLLPVCIALLLSGCLSVYKVEVQQGNVVTQEMIGKLKPGMT
                     RSQVRFVLGTPLVIDGFHPDRWDYYYYLRRSNEDTGETQRLTVIFKNDTLVSVQGGKL
                     DKTESQSPKS"
     gene            complement(5039..5347)
                     /locus_tag="AC16FV_04635"
     CDS             complement(5039..5347)
                     /locus_tag="AC16FV_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005503785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR2849386.1"
                     /translation="MKRKEVWRIEVAYATPLHQEVIEIPARPGTTIEQAIRESGVLER
                     FPEIDLSQHRVGIFGEIARLHDPVHDGDRVEIYRPLLSDPKEVRRRRAKTAGSRKKSK
                     "
     gene            complement(5344..5784)
                     /locus_tag="AC16FV_04640"
     CDS             complement(5344..5784)
                     /locus_tag="AC16FV_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA];
                     GO_process: GO:0045947 - negative regulation of
                     translational initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR2849387.1"
                     /translation="MTAINRSAIVPYSARQMFDLVADIPSYPKFLPWCGGARIVSAQG
                     DEVIAAIDIAYSGVHRTFSTRNLLQRDKMMEIRLLEGPFSYLQGFWQFKALDAQSSKI
                     SLDLEFEVANRIVSLALTPVFSSIANQLVDRFHRRAVELYGNKA"
     gene            complement(5781..7199)
                     /locus_tag="AC16FV_04645"
     CDS             complement(5781..7199)
                     /locus_tag="AC16FV_04645"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR2849388.1"
                     /translation="MIATQKHPARRNPLRSGRKNVHERWSSPGIFVLAAGGAAIGFNN
                     FWQFPHLAAQYGGGAFLIVYLLSVLLIGLPLLMAEFALGRSGRASPIGVFRFMTQRAR
                     ANSLWRLVGWMGVISVFLILSYLSVIAGWVIAYMLRAAFGVYVGLTADGMNAQFAQMV
                     KDPEKQLFWHSLFMTMTMLAAGHGVRRGLEAVVRYAVPLLLGVLLALAAYVATTDAFA
                     QAATVFFLPDFSKLTGMGVLTAMSHAFFSLGLGAGAMLMYGAYLSAEARIPRLSFYVA
                     GVDTLTSMVVSLVVFSILFAGNVELSSGPSLVFQSLPLAFDHIPHGRVLIVVFFALLL
                     IAALTSAIALAEPIMVWLSEQFGMSLRRSAILCGISAWSLGVVTILSFHDWAFSFKLF
                     GITKKLGFFDVMQVVTAHVLLPVSGILIALYAGWVLKPDMMREALHLRAGWVHSTWLW
                     LLRLVIPVLLLIVLFNLPELFA"
     gene            7423..7896
                     /gene="smpB"
                     /locus_tag="AC16FV_04650"
     CDS             7423..7896
                     /gene="smpB"
                     /locus_tag="AC16FV_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR2849389.1"
                     /translation="MSKKKEESHDPVIAQNRKAWHDYFIEDKYEAGLALQGWEVKSLR
                     AGRAQLKEGYVIIDNGEAFLVGAHFSPLTSTSTHIKANPVRSRKLLLHREELNKLIGA
                     TERRGYTLIPLRLYWKHGRAKLEVGLARGKQAHDKRADIKKRESDREMQRALKTR"
     gene            complement(7945..9048)
                     /locus_tag="AC16FV_04655"
     CDS             complement(7945..9048)
                     /locus_tag="AC16FV_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2849390.1"
                     /translation="MSPTLGGFMRKEFKQVLRDRRMWLLLFVTPIIQLTLFGIALSND
                     VKNIRLWAQPDSRDTVLQHVYERAVASKWFLPGAGDRNADPFELLRAGQIDAALIPPA
                     GGLTRALGRGEADLQLLVNAMNVIQAQSVEGYLKNIVNRVVQDDLEVVPPELPVRFDV
                     RVLYNPTLETSYFMVPGVMTILLCMTPLVLTSMSITREKELGTFEMLISAPVTASEVV
                     YGKTMPYVMLGMATVPLILAVAMFGFGVPMRGSLLVLLIASFAFVCTAVAIGTLISTF
                     ARNQQQSMLGGFLFLFPAIMLSGLVFPLENMPWVMKWISYVDPLAHFLALLRNIMLKG
                     GEAGFIALHVAVLVVMAVVSVYVSFKRFHTTLR"
     gene            complement(9045..10160)
                     /locus_tag="AC16FV_04660"
     CDS             complement(9045..10160)
                     /locus_tag="AC16FV_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2849391.1"
                     /translation="MSFRWSRAAAVARKEVYHVLRDPFTLMMSLGLPVFIVFVFGFAI
                     EFNVKDINVAMFDADHTRSSRQLADAFGSSGYFIVDHVASPDAAQRALTGDHDRAALI
                     VPGDFEKDLYAGRTARAQVLLDGSDNSTVGPILSYLGAIQTIASRRIGGFDPPEPVSV
                     KTRFLFNPELNSRWFAVPGLVVAVMAILSVLLTALTVAREWEDGSMELLLSTPVEPLE
                     IIVGKLAPYAVLGIMAVTFTYIMSRTVFGVPFEGSLITFGAGCVLFLGACLAQGLLIS
                     VVMRNQLIALQVAIITGLIPAMLLSGFVFPIESMPAFFRYFTMILPARWFIVITRESY
                     LKGSTLIEMGTAFVALGILCAIMVVLATRKFKKDLEP"
     gene            complement(10157..11083)
                     /locus_tag="AC16FV_04665"
     CDS             complement(10157..11083)
                     /locus_tag="AC16FV_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2849392.1"
                     /translation="MNDAPAVAVSNLTVKFGDFTAVDDVSFTVRRGEVFGFLGANGAG
                     KTTTIRVLCGLLPPTSGEVTVGGVGFDTGEMAIKRKVGYMSQKFTLYNDLTVEENLAF
                     SASLRKLDHATFLKRRDYILDFISFRRPLNSFVRDLPGGIKQQVSLAASMLHDPEIVF
                     LDEPTAGVTPAARARFWALIRDRAAQHKTIFVTTHYMDEAEQCDRIALMRTGRIVALD
                     TPAQLKKAVFPGRLYELDPRDKADYARLQELRRDSGIEYFEAYGLRFHAAFRDTAAGT
                     QLQSRLEQEFHVREIVPTLEDVFIRIVEGANP"
BASE COUNT         2329 a         3481 c         3341 g         2017 t
ORIGIN      
        1 gacccacgag tgcaacagac tgtcacgcct catgttcaat acaacacgaa gcaaaccgcg
       61 attcatcgcc tggctgctgt ccgccatgct gctgtgggtc ggcgcggtgg cggcggcaca
      121 cgagctggat cacgcgctcg atcaccatca cgagctgtgc gcgctgcatc actatgcggg
      181 tcatgatggc ggtctgcctt cctccggtgc tcatccgccg gtaccgccgg tcgaaacccg
      241 tcttgatctt cccaccttac accaatccat ttccaaatcg cgcccggcgc cttatgccgt
      301 gcgcgctcct ccgcaagtct gaagcgcgcc gtcccggcgc ccggtcccgt tatcgcggac
      361 cgttatcggg aattttcata cttcgataaa ccgcgcacaa cgcggatgtg attgtccttc
      421 atccggacgt gcgccaattc agattttgga gaatgtcatg tacaagccca ttttcatggc
      481 gagcctgctg ttcgccgcac acgccgtcca tgctgcggaa ccgccagtaa ctccacaaca
      541 gctgcacgat ctggagcaac gcgtccgaga acttgaagct gcgaatgttg cggcaggcaa
      601 agctgaggct cgtcagacgt caaacaattt caacccggcc atcagcctga ttctcgatgg
      661 caagctatcc ggattttccc gcgatccggc cacttatgca ctgcccggct ttgcgctggg
      721 cgagggagtt ggccccggcg atgaagggtt gcgcctcggc gagtcggaac tggtcatgag
      781 ttccaacatc gacgacaagt tctacggcca attcactgcg gcgctcacgc cggagaacga
      841 aaccgaaatc gaggaagcct ttatcgaaac gttggccctg ggcggtggcg ccaccatcaa
      901 ggccggccgg tttttttctc atattggtta tctcaatccg gtgcatgcac atgcctggga
      961 ctttgcggac cagccattgg tctaccgcgc catgctcggc aaccagtatg gcgatgacgg
     1021 cgcgcagctg cgctgggtgg cgccgacaga cgtgtttctg gaactgggcg ccgagatatt
     1081 ccgcggtgac agctttccgg cgggcggcgc ggccaacagc ggcaatggca cgcgcacggt
     1141 gttcgcgcac atcggtggcg acatcggcgc cgatcatgcc tggcgcgtgg gtttctcgcg
     1201 gcttgatgcc gatgccgtta accgcgccac cgggacggtt gccacacccg atttattcac
     1261 cggcaactcc accgtcaccg gcgtcgatct ggtctggaaa tgggcgccta acggcaaccc
     1321 gaaacagaga aacttcaaat tccagacgga atacttcaaa cgcgacgaga atggcgcgtt
     1381 cgatccggct tcctccggat cgccactgcc ttacaacggg aaacaaacgg gatggtacgc
     1441 acaaacggtg taccagttca tgccgcgctg gcgcgtggga ctgcgacagg atcggcttca
     1501 tgtcgatacg gcaaacgcgg cgctggccgg aatcgtactc gacaacacgg ggcatgaacc
     1561 caaacgcacc agcgcgatga cggacttttc caacagcgaa ttcagccggc tgcggctgca
     1621 gttcaatcgt gacgaatcgc gcgccatgac gaaggacaat cagtggtatt tgcagtatgt
     1681 catgagcctg ggcgcgcatg gtgcgcacgg tttttaagaa aaggaaaaca cacatgaaaa
     1741 tttttcatca attactcgcc gggcttgttt ttctgatcgc gtttccggcc atggcggaga
     1801 ttaaagtctt tgcctgcgag cccgaatggt ctgccctcgt acaggagctg ggtggcgaca
     1861 aggtcacggt gttcacagcc acgaccgcgc ttcaggatcc gcaccacatt gaggcacgtc
     1921 caagcctgat tgcgcgcctg cgccaggccg acctgcttgt ctgcaccggt gccgaactgg
     1981 aggccggatg gttgcccgca ttattgcgtc aagcggggaa cgctgcggtc cagcctggca
     2041 aagcaggtta cttcgaggcc gcgaattacg ttcggaaact ggagattccc gcgcggctgg
     2101 accgtgccga gggcgacgta cacgccgccg gcaacccgca tatccagacc gatccacgca
     2161 atatcggcgc cgtagccacg gcgctggcgc ggtgcctggc ggaaatcgac ccggcgggcg
     2221 ccaaccttta tcaaacacgc cagcgcaatt ttgcgcagcg ctgggatgtc gccatcaaac
     2281 gctgggaaca acaggccgcg cctctcaagg gcgttcatat cgtggtgcaa cacaagggct
     2341 ttccctatct ggaaaactgg ctgggcctac ggcaagtggc agcactcgaa cccaagcccg
     2401 gcgtggaacc gaccagtgcg catctcaccg aggtgctgga acaactgcaa cggcaacctg
     2461 ccaagatgat cctgcgtgcc gcctacagcg acgaccgttc ctcggagtgg ctcgccgggc
     2521 gcgccgggat tcccgccgtc gtgctgccgt tcacagtcgg cggatccgac aaagcgaaag
     2581 atcttttcgg cctgttcgac gataccgtgc agcagttact gggagcacta aaatgaatct
     2641 ggacgcgctc gatatcagca tcctcgggcc cgcgttttgt gcgggcctgc tcgtgctcgc
     2701 gacacatgtg ccgatgggtc agcaggtgct ggcgcgcggc atcgtgttca tcgatctcgc
     2761 gatcgcgcaa atcgccgggc tcggtgtcat cgccgccgat ggcatgggct gggagtcgca
     2821 gggcttcgcg gtgcagatcg cggccgtggg cgcggcgctg ctcggcgcgc tgctcctgac
     2881 cttcaccgag aagcgctggc ccgaggtgca ggaagcgctg atcggcgtgc tgttcgtgct
     2941 cgccgcctgc gccggcatcc tgctgctcgc gggcaacccg catggcggcg agcatctgaa
     3001 ggatctgctc gtcggccaga ttctgtgggt gagctacggc cagctgcttc cggtcctgcc
     3061 actcactgtc ggtatccttg cgctctggtt cggcgcgcgc gatcgattgg ggcgcatcgg
     3121 gttttatgcc atgttcgcgc tcgcggtcac ggcgtcggtg cagctcgtgg ggatatatct
     3181 ggtattcgcc agcctgattc taccggcact cgcaacttac cggctgagaa ccgcacaacg
     3241 gctgacgtgg ggatacggcg cgggcgcggg cggctatgcg attggcatca tggcctcggc
     3301 gttgttcgat cttccgaccg gcgccgtcgc ggtctggacg ctggcactcg tggccttcgc
     3361 cacagccctg ctggtgccgc gtctgaccaa acgcagggaa gttgccggga cgtaaaccct
     3421 gtaagaaaca acgcactcat tttttaatga ctggcaaacc ataatgacag gccggccagc
     3481 accagataca acatgcgccg accccaccgt tccagacgag gccgtattcg tatcggccgg
     3541 gaatcggcga cagcctggac gccagccgcc aatgccttgc ggtaacggaa cttgttatct
     3601 accgggtgca gacgggtata accgtgtttc tcataaaact tgatcgccct ctggttgtgc
     3661 tgcaacacat cgaactccgc ccagcgtaat cccatctgcg cggcccgttc ttcgtagtcc
     3721 cgcagcaatt gcgtccctac tccgccacgg tgacatgaag aaaccacgcc cagataatta
     3781 agccaggccg ttccctcgtc ttttcggggg atgaagtgat agaaaccaac gatcccttca
     3841 cgcgcctcgg caacggcgat agtatcttcg tagttaagta tgtagtgcga aatctgttga
     3901 gccgtgagtt ccggaaaaat ctcccggaac atctccgtca gccttctcca gtccgcccag
     3961 tagattttcc ggatgcgaat ttttatcgcg tcgtggtgca catcggtggc gccggaaacg
     4021 ctggctgaag gcggcgccgc tgatttttcg tggtgaatgc tcatggaccg atcgagatac
     4081 ggaaattatc tcttcgagca cttgccatgt tttttcatgc cgtcgcacac accatagaga
     4141 tacagcgaat ggtcgtcgat atgaaatccg gctttctcgg cgatcttgtg ctggcgttcc
     4201 tcgatggccg tgtcgtaaaa ctcgaacacc ttgccgcatt cgaggcacac catgtggtcg
     4261 tgatgcccgc cctggttgat ctcgaacacc gagcggccgc cctcgaagtt gtgacgcatc
     4321 accaggcccg cggcctcgaa ctgggtcagc acccgataca ccgtcgccag ccccacttcc
     4381 tcgcccatgt tcaggagttc cttgtaaacc tcctccgcgg tcatgtgcgg gtgcccggcc
     4441 gcctccagaa tgcccaggat cttgaggcgc ggcagcgtcg ccttgagtcc ggccttcttg
     4501 agttcggcgc cacctgtatt gacgttacgc atgcgtgata ttcacccggt cctcgaaggc
     4561 ctgcgcaccg ggtatacccg ggcaggtctt cgggttatca tcgccccagt ttaattcagc
     4621 tcgcgaccga ttaccaaaaa tgcgtcattg gctcctgccc gtctgtattg ccctgctgct
     4681 gtccggatgt ctctcggtct acaaagtcga ggttcagcaa ggtaatgtcg tcacgcagga
     4741 aatgatcggc aagctcaagc ccggcatgac gcgcagccag gtccgctttg tgctgggaac
     4801 accgctggtg atcgacggct ttcatcccga ccgctgggat tattactatt atctccggcg
     4861 cagcaacgaa gacactggtg agacccaacg gctcacggtg attttcaaga acgacacgct
     4921 ggtgtcggtg cagggtggca aactggacaa aaccgaaagc cagtccccca aaagctagtc
     4981 cgcctgcccg aaccggaagc ggcacatacc atttaaatcc gctttttgct tcgcgcaatc
     5041 attttgattt tttgcgacta ccggcggttt ttgcacgccg tcttctgact tccttcggat
     5101 cggacagcag cggccggtag atctcgacac gatccccgtc gtggaccggg tcgtgcaatc
     5161 gggcgatctc gccgaaaatt cccacgcggt gttgcgacaa atcgatctcg ggaaagcgtt
     5221 caagcacgcc ggactcgcga atggcctgtt cgatggtggt gcctggacgg gccggtattt
     5281 caatcacctc ctgatgcaat ggcgtggcat aggccacctc gatgcgccag acttctttcc
     5341 gcttcacgcc ttgtttccgt acagctcgac ggcacgccgg tgaaaccggt ccaccagctg
     5401 gttcgcgata ctggaaaata ccggcgtgag cgccaggctc acgatccggt tcgcgacctc
     5461 gaattccaga tcgagggaga tcttgctgga ttgcgcgtcc agtgccttga attgccaaaa
     5521 gccctgcaaa taactgaagg gtccttcgag cagccgaatt tccatcatct tgtcgcgttg
     5581 cagcaggttg cgcgtggaaa acgtccggtg cacaccgctg taggcgatat cgatggcggc
     5641 gatcacctca tccccctgtg ccgagacgat gcgcgcgccg ccacaccatg gcaggaattt
     5701 cggataggac ggaatgtccg ccaccagatc gaacatctgg cgcgcactgt aaggcacgat
     5761 ggcggagcga ttgatcgcgg tcacgcgaac agctcgggaa gattgaacag gacgatcagc
     5821 agcaaaacgg ggatcacgag ccgcagcagc cacagccacg tggagtgaac ccagccggcg
     5881 cgcagatgca gcgcctcgcg catcatgtcg ggtttcagca cccaaccggc ataaagcgcg
     5941 atgagaatac cggagaccgg caacaacacg tgcgccgtca ctacctgcat cacgtcaaaa
     6001 aagccgagtt ttttggtaat cccgaacagc ttgaaggaaa acgcccagtc atgaaaagac
     6061 agaatcgtga cgacacccag ggaccaggcg ctgatcccgc acagaatcgc cgagcgacgc
     6121 aggctcatgc cgaattgttc gctcagccag accatgatcg gttcggccag cgcaatcgcc
     6181 gaggtcaggg cggcgatcaa caacagtgca aaaaaaacga cgatcaatac acggccgtgg
     6241 gggatgtggt cgaacgccag cggcaacgac tggaacacca gactcggacc agaggacagt
     6301 tccacattgc cggcgaacaa tatcgaaaac accaccagac tgacgaccat gctcgtgagc
     6361 gtatccacgc ccgccacata gaatgaaagc cgcggaatgc gcgcctcggc gctgaggtac
     6421 gcgccataca tcagcatggc gcccgcgccc agacccaggc tgaaaaaggc gtgactcatg
     6481 gcggtgagca cgcccatgcc ggtgagcttg ctgaaatccg ggaggaaaaa taccgtggcg
     6541 gcctgggcaa aagcgtccgt cgtggcgaca taggccgcca gggccagcaa cacccccagc
     6601 agcaacggca cggcatagcg cacgaccgct tccaggccgc ggcgcacgcc gtgaccggcc
     6661 gccagcatgg tcatggtcat gaacagcgag tgccaaaaaa gctgtttctc gggatccttc
     6721 accatctgcg cgaactgggc attcatgccg tccgcggtca aaccgacata gacgccgaac
     6781 gcggcgcgca gcatgtaggc gatgacccaa ccggcgatga cgctgagata agagagaatc
     6841 agaaacacgc tgatgacgcc catccatccc accagcctcc acaatgaatt cgcgcgcgcc
     6901 cgctgggtca tgaaccggaa aacaccgatc ggcgaagcgc gcccgctgcg ccccagggcg
     6961 aattccgcca tcagcaacgg caggccgatc aggagcacgc tcagaagata aacaatcaga
     7021 aacgcgccgc cgccgtattg ggcggccaga tggggaaact gccagaaatt gttgaagccg
     7081 atggctgccc cgcccgcggc cagcacgaaa atgcccggcg aggaccagcg ctcatgcacg
     7141 tttttcctgc cggaacgcag agggttgcgg cgcgcgggat gtttttgcgt tgcgatcatt
     7201 atgatccggt caccgatgcc aggcttcatt cctctcggcc gggcaagaat gtggcatact
     7261 cgccgtgggc cgtccaccga caaacagctt catgcgcatc ggcatcatga tgctcattcc
     7321 tttatatatt gataagcata ggccaacagc gggatcgaat cggtacgcgt atccggcggt
     7381 ttcgcgcgac aataacggcc gtcacggcat gttcacgaga gaatgagcaa gaaaaaagaa
     7441 gagtcgcacg atcccgtcat cgcccagaac cgcaaggcct ggcatgacta tttcatcgag
     7501 gacaaatacg aagccgggct ggccctgcag ggctgggagg tcaaaagcct gcgcgccggg
     7561 cgtgcgcaac tgaaagaagg ctacgtgatc attgataacg gcgaggcctt tctggtcggc
     7621 gcgcatttct cgccgctcac gtccacctcc acccacatca aggcgaatcc cgtgcgcagc
     7681 cgcaaactgc tgttgcaccg cgaggagttg aacaagctga tcggcgcgac ggagcggcgc
     7741 ggttacacgc ttattccatt gcggctctac tggaagcacg gccgcgccaa gctggaggtc
     7801 ggcctcgcgc gcggcaagca ggcgcatgac aagcgcgccg acatcaagaa acgcgaatcc
     7861 gaccgcgaaa tgcagagggc gttgaagacc cgctgacggc acaggaccgg tctttcgccg
     7921 gcgcgcgctg cgcgatgatg acggttaccg cagcgtggtg tgaaatcgct tgaagctgac
     7981 gtaaaccgaa accaccgcca tgacgaccag caccgccacg tgcagcgcaa taaagccggc
     8041 ctcgccgccc ttgagcatga tgttgcgcag cagtgccaag aaatgggcca ggggatcgac
     8101 gtaggaaatc cacttcatga cccacggcat gttctccagc ggaaacacga gccccgaaag
     8161 catgatcgcc ggaaacagga acaggaaccc cccgagcatg gactgctgct ggttacgtgc
     8221 gaacgtggag atcaaggtcc cgatggcgac cgcggtacag acgaacgcga acgacgcgat
     8281 tagcagcacc aacagcgagc cgcgcatggg cacgccgaac ccgaacatcg ccaccgccaa
     8341 gatcaggggc acggtggcca tgccgagcat cacgtaaggc atggtcttgc cgtagaccac
     8401 ctccgacgcc gtgaccggcg ccgaaatcag catctcgaag gtgcccagtt ctttttcgcg
     8461 cgtgatcgac atgctggtca acaccaacgg cgtcatgcac agcaatatgg tcatcacgcc
     8521 ggggaccatg aaataggagg tttcgagcgt cgggttgtac agcacgcgca cgtcgaaccg
     8581 gaccggcagc tcgggcggca cgacctcaag gtcgtcctgg accacgcggt tcacgatgtt
     8641 cttcagatag ccctcgacgg attgcgcctg gatcacgttc atggcgttga ccagcagttg
     8701 caaatcggct tcgccgcgcc cgagtgcgcg cgtaagtccg cccgcgggtg gtatcagcgc
     8761 ggcgtcgatc tggcctgcgc gcagcagctc gaaggggtcg gcgttgcggt caccggcgcc
     8821 cggcaggaac cacttactcg ccaccgcgcg ctcgtagacg tgctgcagga ccgtgtcgcg
     8881 cgagtccggc tgcgcccaca gccggatgtt cttgacgtcg ttcgagagcg cgatcccgaa
     8941 caacgtgagc tggatgatcg gggtgacgaa cagcaacaac cacatgcgtc ggtcgcgcag
     9001 cacctgcttg aattccttgc gcatgaaacc gccgagcgtg gggctcatgg ctcgaggtct
     9061 ttcttgaact tgcgggtcgc gagcacgacc atgatcgcgc acaggatacc gagcgccacg
     9121 aacgccgtgc ccatctcgat cagggtcgag cccttgaggt aggactcgcg cgtgatgacg
     9181 atgaaccagc gcgccggcag gatcatggtg aaataccgga aaaaggccgg catactttcg
     9241 atcggaaaga caaaaccgga aagcagcatg gccgggatca acccggtgat gatcgcgacc
     9301 tgcagggcga tcaactgatt gcgcatgact acggagatca gcagcccctg cgccagacac
     9361 gcgccgagga acagcacgca accggcgccg aaggtgatca ggcttccctc gaacggaaca
     9421 ccgaatacgg tccgtgacat gatgtatgtg aacgtcaccg ccataatgcc gagcaccgcg
     9481 tagggcgcga gcttgcccac gatgatctca agcggctcga ccggcgtgga cagcagcagc
     9541 tccatcgagc catcctccca ttcgcgcgcc accgtgagcg ccgtgagcag caccgaaagg
     9601 atcgccatca cggccaccac gaggcccggg accgcgaacc agcggctgtt gagctcggga
     9661 ttgaagagaa aacgcgtctt gacgctgacc ggctcgggcg gatcgaaccc gccgatgcgg
     9721 cgcgaagcga tggtctggat cgccccgagg tagctgagga tcgggcccac ggtcgagttg
     9781 tcggagccgt cgagcaacac ctgcgcccgc gccgtgcggc cggcgtagag gtccttctcg
     9841 aaatccccgg gaacgatgag cgccgcgcga tcatggtcgc ccgtcagtgc gcgctgcgcg
     9901 gcgtccggcg aggcgacgtg atcgacgatg aaatagccgg aactgccgaa ggcgtcggcg
     9961 agctggcgcg acgagcgggt gtggtccgcg tcgaacatcg cgacgttgat gtccttgacg
    10021 ttgaactcga tcgcgaaccc gaacacgaac acgatgaaca ccggcagtcc gagcgacatc
    10081 atgagcgtga acgggtcgcg caacacgtga tacacctcct tgcgcgcgac cgcggcggca
    10141 cggctccagc gaaagctcat gggttggcgc cctccacgat ccggatgaac acatcttcga
    10201 gcgttggcac gatctcgcgc acgtgaaact cctgctcgag ccgggattgc agctgcgtcc
    10261 ccgctgcggt gtcgcggaac gcggcgtgga accgcaatcc atacgcctcg aagtattcga
    10321 tgcccgagtc gcgacgcagc tcctggagac gcgcgtaatc ggctttgtcg cgcgggtcga
    10381 gctcgtacaa ccgtcccggg aaaaccgcct ttttaagctg cgccggcgtg tcgagcgcga
    10441 cgatccgtcc agtgcgcatc agcgcgatgc ggtcgcactg ctcggcctcg tccatgtagt
    10501 gcgtggtgac gaaaatcgtc ttgtgctgcg ccgcgcggtc gcggatcagc gcccagaagc
    10561 gcgcccgcgc cgccggcgtc acgcccgcgg tgggttcgtc gagaaacacg atttcgggat
    10621 cgtgcagcat cgaggccgcg agcgagacct gctgcttgat gccgccgggc aggtccctaa
    10681 cgaacgagtt cagcggccgc cggaaggaga taaaatccag aatgtaatcg cggcgcttca
    10741 gaaaggtcgc atggtccagc ttgcgcaggc tggcgctgaa cgcgaggttc tcctcgaccg
    10801 tgagatcgtt gtacagggta aacttctgcg acatgtaacc gaccttgcgc ttgatggcca
    10861 tctcgccagt gtcgaagccg acgccgccga ccgtcacctc gcccgaggtc ggcggcaaca
    10921 gtccgcacaa cacccggatc gtcgtggtct tgccggcgcc gttggcgccg aggaacccga
    10981 agacctcccc gcgccttacc gtgaagctga cgtcatccac cgccgtgaag tcgccgaact
    11041 tcaccgtgag gttcgaaacg gcgacggccg gcgcgtcgtt cacgacacgg cctccgcgtg
    11101 cctgaggaac agctcgtcga acgtgcgcac gcgttcgcgc gcgagcagcg ctctcggctc
    11161 gccttcgg
//
LOCUS       DBNGZE010000176         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_442498, whole genome shotgun sequence.
ACCESSION   DBNGZE010000176 DBNGZE010000000
VERSION     DBNGZE010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_442498"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1249
                     /gene="arsS"
                     /locus_tag="AC16FV_04670"
     CDS             278..1249
                     /gene="arsS"
                     /locus_tag="AC16FV_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551656.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR2849393.1"
                     /translation="MRDTRPLLDKIPFPAIRRRKLETLQVNVGYRCNQSCFHCHVNAG
                     PHRTEEMTGEIADLVLDFLRRRRVAILDITGGAPELNPHFRRLVQEARMLGVKVIDRC
                     NLTILEQPGQEDLAEFLASEHVEVVASMPCYLQDNVERQRGKGVFEGSIRGLKRFNAL
                     GYGRDPELALNLVYNPQGPLLPPAQASLEADYKRVLREKYGIVFNKLYVLANMPIQRF
                     GVTLIKMGEFYAYLGLLQDAHLDANLDGVMCRSLISMDWRGYIYDCDFNQMLDLPMAR
                     GTRRHVHLADLLNDSIEGNPIRVAGHCYGCTAGQGSSCGGALKEAAE"
     gene            1246..1944
                     /locus_tag="AC16FV_04675"
     CDS             1246..1944
                     /locus_tag="AC16FV_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR2849394.1"
                     /translation="MSGTRLSIIIPVLDEAPFIEAALAALSPYRARGVEVIVVDGGSR
                     DGTLAVARPLADCVLSTRCGRATQMNAGAAIARGEILLFLHVDTWLPDDADILVHDGL
                     ARSRRAWGRFDVRFDHGGWLSLIAFIMNLRSRWTGIATGDQALFVTRAAFDATGGFPT
                     IALMEDIALSTNLKRISWPFCIRARATTSARRWRQYGTVRTTFLMWRLRMAYFFGADP
                     AQLARHYGYAPGEQ"
     gene            1925..>2430
                     /locus_tag="AC16FV_04680"
     CDS             1925..>2430
                     /locus_tag="AC16FV_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR2849395.1"
                     /translation="MPLASNSPVSIAILAKAPIPGLAKTRLIPAIGAHAAAVLQERLT
                     ERTVTTALAANIGPVTLWCAPDSTHSTFLRLVARLRITLKPQPQGDLGARMLAAAAAC
                     SGPALVIGTDCPALTEVHLRGAANDLRDGTDVILIPADDGGYVLIGTRSEQPALFAGI
                     AWGTKTVLA"
BASE COUNT          446 a          739 c          726 g          519 t
ORIGIN      
        1 gcctagtaag cggcggctcc tgaccctcaa cgaactttcg cgtgccggcg gccgttttct
       61 tgtcgccatt gctacggcag cacgacgtta gcctcccggc agcatgatcc gcgccgtatg
      121 ccaatcgtgc cagacttggt cagagaacca gcaagcggac gagatcggtc gtagcgccaa
      181 attttacaag cgtatcacgg cattttgtcc cgccgccatt tcgttcgggg tgacgtgggc
      241 gcgtgcttca tggcaagttg tgcggattga ggccccgatg cgcgacacac gtccgctcct
      301 cgacaagatt cctttcccgg cgatccggcg ccgcaagctg gaaacgctgc aggtgaatgt
      361 tggctatcgc tgtaaccagt cctgcttcca ttgccacgtc aatgccggcc ctcaccgaac
      421 cgaggagatg acaggcgaga tcgctgatct ggttttggat tttcttcgcc gccgaagagt
      481 cgcgatcctc gacattacag gtggcgcgcc ggagcttaac ccgcatttcc gacggttggt
      541 gcaagaagcg cgcatgctgg gcgttaaggt aattgatcgc tgcaatctca ccattctgga
      601 gcagccaggc caggaggacc ttgcagaatt tttggcgagc gagcacgtcg aggtggtggc
      661 gtcaatgcca tgctatctgc aagacaatgt cgagcggcag cgcggcaagg gtgttttcga
      721 gggctcgatc cgtggcctaa agcgcttcaa tgcgctcggc tacggtcgcg atcccgagct
      781 cgcgctcaat ctcgtttaca atccgcaggg accattgctg ccgccagcgc aggcatctct
      841 cgaagccgac tacaagcgcg tgctgcgcga gaaatatggc atcgtgttca acaagcttta
      901 tgtcctcgcc aacatgccga ttcagcgctt tggagtcacg ctaatcaaaa tgggcgagtt
      961 ctacgcttat ctcggcttac tacaagatgc acatctcgac gccaatcttg atggtgtcat
     1021 gtgccgcagc ctgatttcga tggactggcg aggctacatc tatgattgcg attttaacca
     1081 aatgctcgac ttaccgatgg cgcgcggcac gcgcaggcat gtgcatttgg cagatttact
     1141 taacgactcg atcgagggca atccgatccg cgttgcgggc cattgctatg gttgtactgc
     1201 aggccagggc tcgagctgcg gcggcgcgct gaaagaggcg gcggaatgag cggcacgagg
     1261 ttgtcgatca tcataccggt attggatgaa gctcccttca tcgaagcagc gctggcggcg
     1321 ctttcgccct atcgagcccg cggcgttgag gtgatcgtcg ttgatggcgg aagccgcgat
     1381 ggcacacttg cagtcgcccg ccccctcgcc gactgcgtgc ttagtacgcg ctgcgggcgc
     1441 gcgacgcaga tgaatgctgg cgctgcaatt gcgcgcggcg aaatcctgtt gttcctgcat
     1501 gtagacacat ggctgcccga cgacgccgat attctagtac acgatgggct cgcgcggtcg
     1561 cgccgcgcct ggggccgttt tgatgtgcgg tttgaccacg gcggatggct gtctttgatc
     1621 gcgtttataa tgaatctgcg gtcgcgttgg acgggcatcg ctacgggcga tcaggccctc
     1681 ttcgtaaccc gcgcggcctt tgatgccacc ggcggctttc cgacgatcgc gctgatggaa
     1741 gatattgcgc tgtccaccaa cctcaagcgc atcagctggc cattttgcat ccgtgcccgc
     1801 gctaccactt ccgcacgtcg ttggcgacag tatggcaccg tgcgcacaac gtttctgatg
     1861 tggcggctgc ggatggcata tttcttcggt gctgatccag cgcaacttgc acggcactat
     1921 ggctatgccc ctggcgagca atagtcccgt ttccattgcg atcctggcga aagccccaat
     1981 cccggggctg gccaagactc gccttattcc agcaatcgga gcgcatgcag cggcggtgct
     2041 acaggagcgc ctgacagagc gcacggttac gactgcgctc gccgccaata ttggccccgt
     2101 gacgttatgg tgcgcacccg acagcacgca cagcactttc ctcaggttgg tcgcacgcct
     2161 ccgcatcacg cttaagccgc agcctcaagg tgacctcggc gcgcgcatgc tcgccgctgc
     2221 agccgcctgt agcggccccg cgctggtgat cggtactgac tgccctgcgc tgaccgaagt
     2281 gcaccttcgc ggagcggcga acgacctccg cgacggcact gatgttatct tgatccccgc
     2341 cgatgacggc ggttatgtcc tgatcggcac acgctcggag cagcccgcgc tgttcgcagg
     2401 catcgcctgg ggcacaaaaa ctgtgctcgc
//
LOCUS       DBNGZE010000177         2191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_998674, whole genome shotgun sequence.
ACCESSION   DBNGZE010000177 DBNGZE010000000
VERSION     DBNGZE010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_998674"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="AC16FV_04685"
     CDS             complement(<1..841)
                     /locus_tag="AC16FV_04685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2849396.1"
                     /translation="MKPSKVQSPPEKDFHLSLLRAYIDSANDGIFVVCDEMKFHVANR
                     LLAEWLGETEKSLTAHKQRLPITRFFGDAEDKKLFKERFQSVLDGKPVRFECHLSPKH
                     GEARWVEISMNKVDLEAGDLVIGVVRDITERRAMLAEAHHHARHDDLTGLFNRREFER
                     RMKELFQFTQSENARHVLLYVDLDQFKIVNDSCGHMAGDELLRQIAARTQKEVRKNDT
                     FARLGGDEFGVLLENCQMDQALEIAEKLRESIAGFRFRWNDQVFDISASIGVCAITQA
                     SEST"
     gene            1214..1600
                     /locus_tag="AC16FV_04690"
     CDS             1214..1600
                     /locus_tag="AC16FV_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849397.1"
                     /translation="MNHPAANSSKRLLLSIIELGGYPDFKPLYRQAGYEVESAASVRK
                     ALGLLKKLSPDVIVAEFNFQSDFRDRTSSLESLLAVVQRLPKTRVIVFYEKDHAHQLT
                     RLTNQYPIHAAITYPIDTQLLDASLR"
     gene            complement(1577..>2191)
                     /locus_tag="AC16FV_04695"
     CDS             complement(1577..>2191)
                     /locus_tag="AC16FV_04695"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2849398.1"
                     /translation="IVKAGWQAVSERDGLRLYNRAVPGASVPAALARVRIEVPPAHLF
                     AVITDYDHFAEFVPYVTRSRVVRREDGLRRVSQHLRFPGPVADRHYVIESRDSVTPSP
                     RERWRVEWRLVPESDSPASGETGITPNAFTGYWELTPIAGGTATDAIYSLHFDPGGAL
                     PAWLVTFAMNRYLPKVIDAVRARATGSDRLSPTPDARLSKTRVE"
BASE COUNT          405 a          663 c          659 g          464 t
ORIGIN      
        1 cggtactttc gcttgcctgt gtgatggcgc aaacgccgat gctggcgctg atgtcgaata
       61 cctggtcatt ccaccggaaa cggaacccgg cgatgctctc tcgcagtttc tccgcgatct
      121 ccagcgcctg gtccatctgg cagttttcca gcagcacgcc gaactcgtcg ccgcccagtc
      181 gcgcgaaggt gtcgttcttg cgcacctcct tttgtgtgcg cgcggcgatc tggcgcagca
      241 attcatcgcc cgccatgtga ccgcaactgt cgttgacgat cttgaactgg tccaggtcca
      301 cgtagagcag gacgtgccgg gcattctcgg attgcgtgaa ctggaacagc tccttcatcc
      361 gccgctcgaa ctcgcgccgg ttgaacaggc cggtgagatc gtcgtgccgc gcgtggtgat
      421 gcgcctccgc cagcatcgcg cggcgctctg tgatgtcgcg caccacgcca atgacaagat
      481 cgccggcttc gagatccacc ttgttcatgc tgatttccac ccagcgcgct tcgccatgct
      541 tgggactcag atggcactcg aaacgcaccg gtttgccgtc gagcacggat tgaaatcttt
      601 ccttgaagag tttcttgtct tcggcgtcgc cgaagaaacg ggtgatgggt aatcgttgct
      661 tgtgcgccgt cagggatttc tccgtctcgc caagccattc ggcgagcagg cggttggcca
      721 cgtgaaattt catctcgtcg cacacgacga aaatgccatc gttcgcgctg tcgatgtaag
      781 cgcgtaggag ggaaagatgg aaatcctttt ccgggggcga ctggaccttt gacggtttca
      841 cagagatacc tccggcgcga cgaaacatcc ccggcaagac cgggaccgcc gcatttctta
      901 aatatagttc aagcatgatt tatttcctgc tcagtactgg caaatccggc ggcaaggcca
      961 tgttttgaca tttggcgtct cccgcgggtg gtctcaggca gctcgtcgtt tccagcgttt
     1021 cgcgtagctg taaccaccta aaaactgaca ggattccccg tttccgtggg agagacggca
     1081 tttcctccgc ttggtgggga aaagcggctt tgccgggggg tcatttaact ctttccattc
     1141 gtcgcagggt attccaccag ccgcggttgc caaatcgcac ggttgcggag atgatgcggg
     1201 ccggagtcca agcatgaatc atcccgccgc gaattcttca aagcgcctgc tgctttccat
     1261 catcgagctt gggggatatc ccgatttcaa accgctttac cgtcaggccg ggtatgaagt
     1321 ggaatcggcg gcctcggtgc gcaaggcgtt gggcttgctg aaaaaactca gccccgacgt
     1381 cattgtcgcc gaattcaatt ttcagtcgga tttccgtgac cgcaccagca gcctggaatc
     1441 gttgctggcg gtggtgcaac gcttgccgaa gaccagggtg attgtttttt acgaaaaaga
     1501 ccacgcgcac cagctcaccc ggctcacgaa ccagtacccc atccacgccg cgattactta
     1561 tccgatcgac acgcagctac tcgacgcgag tcttcgataa acgtgcgtct ggagttggcg
     1621 acaatctatc cgatccggtg gcccgggcgc gcacggcatc gatgaccttg ggcagatagc
     1681 gattcatcgc gaacgtcacg agccaggcgg gcagggcgcc gccggggtcg aagtgcagcg
     1741 aatagatcgc gtcggtggcc gtgccgccgg cgattggtgt cagttcccag tagcccgtga
     1801 acgcgttcgg cgtgattccc gtttcgcccg aggcgggtga gtcgctttcc ggaaccaggc
     1861 gccattcgac gcgccagcgt tcccgtggcg agggtgtgac actgtcgcgg ctttcgatga
     1921 cgtaatggcg gtcggccacg ggcccgggaa aacgcagatg ctgggatacg cggcgcagcc
     1981 catcttcacg gcgcaccacc cggctgcggg ttacgtacgg cacgaactcg gcgaagtggt
     2041 cgtaatccgt gatgaccgcg aacagatgcg ccggcgggac ttcgatgcgc acacgcgcca
     2101 gcgcggcggg tacgctggcg ccggggacgg cgcggttata gagccgcaga ccgtcccgct
     2161 ccgacaccgc ttgccagccg gccttcacga t
//
LOCUS       DBNGZE010000178         3421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1063352, whole genome shotgun sequence.
ACCESSION   DBNGZE010000178 DBNGZE010000000
VERSION     DBNGZE010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1063352"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="AC16FV_04700"
     CDS             complement(<1..1465)
                     /locus_tag="AC16FV_04700"
                     /inference="COORDINATES: protein motif:HMM:NF017081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HIR2849399.1"
                     /translation="MSREATAAKESIKKISALALLTLFYFAAGKLGLALAIVNPSATA
                     VWPPTGIAIAALLVLGYRAWPAILAGAFLVNITTSGSIPASLMIAGGNALEALAGAYL
                     INRYANGRRVFERAQDIFRFIVLAGMASTAISATIGVASLLLNGLVPPSNVPHVWLTW
                     WLGDATGALIVAPVLILWANYIRDQDRTRVLEAALLMLSLLVVGVIAFSDLSLADIRS
                     YPVYLLALPVVIWFAFRVGSPETATAILILSGIAISSTFAGSGPFSSYPANEALLLLQ
                     GCMSIVAITFLSLAAVVAERRRTEQALAHSEDKYNAVVETARDVVVTINERSEILYIN
                     AAAEKLFGYRVAEMIGQNLSMLMPERLRSRHRTSFQHYLHTGQRQIKWDGVELIGRHK
                     DGHEIPLEVSFGETSHGGMRLFTGVMRDITRRKQVEESNRWLATLVESSGDAVIGKTL
                     DGVVLSWNKGAEKIYGYTAGEMIGRPISILMSPDRLDD"
     gene            1943..2827
                     /locus_tag="AC16FV_04705"
     CDS             1943..2827
                     /locus_tag="AC16FV_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2849400.1"
                     /translation="MSSNDTRRFYPVAALLIGASMWGVIWYPMRLLEGGGLGGIWLTL
                     TLYGAALAVSLPFTLKSISEFTRCPGWLAMLMVTAGWTNIAFVEAVLEGNILRVLLLF
                     YLSPLWATLMAWAFLREHITRMGFASLVIAMGGALLMLWNPILGAPWPQGKADWMALT
                     AGFAFALSNTVTRKLHDVSIEAKVLSVWGGVSIVALVMITMFSLPMPQPSLTIFTGAV
                     ALGLFGILFMTLLVQYGVTHMPVHRSAVIALVELVAGAIAQQLLTDEIVTLREWVGGG
                     LIVIGAFLSARASSSRDY"
     gene            complement(2985..>3421)
                     /locus_tag="AC16FV_04710"
     CDS             complement(2985..>3421)
                     /locus_tag="AC16FV_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR2849401.1"
                     /translation="MTTGMRIHELTLEQQELWQRVNDLWALSLTRNATEIRNTLHPRY
                     VGWDMSSPAPHDREAAVQSVLGDSPVVTQYQLTPLSVEVYDHTVGIVHYAYSATVAPR
                     EAAPLQVTGKWTEIYLRQDQQWVMVGVSGRPDPQAGACNASS"
BASE COUNT          643 a         1011 c         1037 g          730 t
ORIGIN      
        1 tgtcgtcgag acggtcggga gacatgagaa tcgagatcgg gcggccgatc atttctccgg
       61 cggtgtaacc gtatattttt tccgcgccct tgttccaaga caggacgacc ccgtccaggg
      121 tcttgccgat aaccgcgtca cccgaggatt ccaccaatgt cgccagccag cggttggatt
      181 cttccacctg ttttcgccta gtgatgtcgc gcatcacgcc ggtgaacagg cgcatgccgc
      241 cgtgagaggt ttcgccgaac gatacctcga gagggatttc gtgcccgtcc ttgtgccggc
      301 caattagttc aacgccatcc catttgatct gtctctgccc ggtatggaga tagtgctgga
      361 atgacgtccg gtgcctggac cgcagccgtt ccggcatcag catggacagg ttctggccga
      421 tcatttcggc aacgcgatag ccaaacagtt tctcggccgc cgcgttgatg tagagaatct
      481 cgctgcgctc gttgatggtg acgacgacgt cgcgagcggt ctcaaccacg gcgttgtatt
      541 tgtcctcgct gtgggccagc gcctgttccg tgcgccggcg ttccgcgacc acggccgcca
      601 ggctcagaaa ggttatcgcc acgatgctca tgcaaccctg cagcaacagc agcgcctcat
      661 tcgccggata gctggaaaac ggtccgctgc ccgcgaacgt gctggagatg gcgatgccgg
      721 acaggatcag aatcgcggtc gcggtttcgg gtgaaccgac gcggaaggca aaccagatca
      781 ccaccggcag cgccagcaga taaaccgggt agctccggat gtcggcaagg gacaagtcgc
      841 tgaaggcgat cacgccgacg accagcaacg acagcatcaa caaggctgcc tccagtaccc
      901 gcgtccggtc ctggtcgcga atgtagttgg cccagagaat gagcaccggc gcgacgataa
      961 gggcgccagt ggcgtcaccc agccaccagg tcagccagac gtgagggaca ttggacggcg
     1021 gcacgagtcc gttcaggagc aggctggcca cgccgatggt tgcgctgatg gccgtgctgg
     1081 ccatgccggc aagcacgatg aaccggaaaa tatcctgcgc acgctcgaac acgcgccggc
     1141 cgttggcgta acggttgatc aggtacgccc cggcgagggc ttcgagcgca ttgccgcccg
     1201 cgatcatgag cgatgccggg atcgagccgg aggtggtgat gttgaccaga aacgcgcccg
     1261 cgagaatcgc cggccaggcg cggtaaccga ggaccagcag cgcggcaatc gcgattcccg
     1321 tcggcggcca caccgccgtg gcgctgggat tcacgatcgc cagtgccagc cccagcttgc
     1381 ccgctgcgaa atagaacagt gtcagcagcg ccagcgccga tatttttttt attgattctt
     1441 ttgccgctgt agcttcccgt gacacgggca tttctcctca ggccggctca gccgcacgca
     1501 ccgttttagg tggataaaag ggtcgagtcc gtgacggatt ttcacaatct acatagaatt
     1561 caagtgtagg ccagcttcgc ccggcaggaa gtaacggaaa cgatattagg ggtacttaat
     1621 ataggggaaa ggcggtgaaa attgagcata tttgcatgat ggtggcgtaa ttgccgctcg
     1681 catcaattta taccacgcgc tccggatcag ccccgtctta tcaatgtgac gcgcgcagat
     1741 catccggcga tggcccggtg cagctcggcg agcccttggt caccgggtaa tgcgacagcc
     1801 cgccaagatt ggacctgagg gcttccaaaa caaaagcggg tgcggcatgc tgcggctcgc
     1861 ccagggaggg ccgtaagctc gcgagtcggg aattcatgga gtccggtttg tttagaaaaa
     1921 agaaatatac ggaaagcccc tgatgtcctc caacgacacg cggcgttttt atccggtagc
     1981 ggccctgctg atcggcgcca gcatgtgggg cgtgatctgg tatccgatgc gcctgctcga
     2041 aggcggcggc ctcggcggca tctggctcac gctcacgctc tatggcgcgg cgctcgccgt
     2101 gagcctgccg ttcacgctca aatccatttc ggaattcacc cgctgtcccg gctggctcgc
     2161 catgctcatg gtgacggccg gctggaccaa catcgccttc gtcgaggcgg tgctggaagg
     2221 caatatcctg cgcgtgctgc tgctgttcta tctgtcgccg ctgtgggcca cgctcatggc
     2281 ctgggcgttt ctgcgcgaac acatcacgcg catgggtttc gccagcctcg tcatcgccat
     2341 gggcggcgcg ttgctgatgc tgtggaatcc gatcctcggc gcgccctggc cgcagggcaa
     2401 ggccgactgg atggcgctca ccgcgggctt cgccttcgcg ctgtccaata ccgtcacgcg
     2461 caagctgcac gacgtgtcca tcgaggcgaa agttttgtcc gtctggggcg gcgtcagcat
     2521 cgtggcgctg gtcatgatta ccatgttttc cttgcccatg ccgcagccgt ctctcaccat
     2581 cttcaccggc gcggtggcgc tcggcctgtt cggcatcctg ttcatgacgt tgctggtgca
     2641 atacggtgtc acgcacatgc cggtgcaccg ctcggcggta attgccttgg tcgagctcgt
     2701 cgctggcgcg attgcacagc agcttcttac agatgagatt gttactttga gggagtgggt
     2761 agggggaggg ttgattgtta ttggggcgtt tttgtcggcg agggcatcat cgtcgaggga
     2821 ttattagttt ttctctcctt accttttctg tcattcccgc gtaggcggga atccaggtag
     2881 taaatggttc gcgcgctttg cgcgcgatct caaaaacata attgcgggtg gctcccgccc
     2941 ctgcaccctg gggtccattt cttttgttgg gccgaaccat gcacctacga tgacgcattg
     3001 catgctcccg cttgaggatc aggccgccca ctgacaccaa ccatgaccca ttgttggtct
     3061 tgcctcaggt atatctcggt ccactttccc gttacttgta gaggtgccgc ctctcttggc
     3121 gccacagtgg cggaataggc gtagtgaaca ataccgaccg tatggtcgta tacctcgaca
     3181 ctcaacggag tgagttgata ctgagtaact accggcgaat cccccagcac ggactgaacg
     3241 gcggcttctc tgtcatgagg tgctggactg ctcatgtccc agccgacata tcgaggatga
     3301 agcgtgtttc gaatctctgt ggcgttgcgc gtaagtgaca gagcccacaa gtcattgact
     3361 ctctgccaca gttcctgctg ctccagcgtg agttcgtgaa tgcgcatacc ggtcgtcatt
     3421 g
//
LOCUS       DBNGZE010000179         5932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_442883, whole genome shotgun sequence.
ACCESSION   DBNGZE010000179 DBNGZE010000000
VERSION     DBNGZE010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_442883"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..975)
                     /locus_tag="AC16FV_04715"
     CDS             complement(7..975)
                     /locus_tag="AC16FV_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HIR2849402.1"
                     /translation="MTKTTSIHGAKIRLGISSCLLGQKVRFDGNHKLDSFLTGTLGQF
                     FEWVPVCPEVAIGLGVPRAPIRLVGNASAPRAVGVKDASIDVTDKLAAYGKQQARKLD
                     DLSGYVFKSRSPSCGMKRVKIYQHAGVPAKSGRGIYADAFLAGQPWLPAEEEGRLGDP
                     RLRENFIERVFVYRRWREYTSHGLTAARLVEFHTRHKLALMAHDMEAYRVLGRLVAQA
                     GRRNLKESGREYLLHLMQAFQRLATPARHANVLMHLMGYLKKYLDAEDKAELLGMIHA
                     YRQGEAPFAAPLILLKHHFRRHPDPYIAGQTYLNPDPRELLLRGGL"
     gene            complement(1098..1745)
                     /locus_tag="AC16FV_04720"
     CDS             complement(1098..1745)
                     /locus_tag="AC16FV_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849403.1"
                     /translation="MRNRCISVCAVLMLPWFTNVAHGDVGINLKAGTLGAGVELSKSF
                     SDKFSVGLGFNSYDFKTTDSASDIDYDFNFDLQSVALFANYHPFGGAFHFTGGAVYNK
                     NELNLIGKPTGTNYVINGQTYTAAQVGTLTGQLTFNSTAPYLGLGWGNRPGGRLGLSA
                     DIGVLYQGSPKLALSATGAAAGLASDVEQERRSAEEDLSDLKWYPVLSLGMYFRF"
     gene            1890..2630
                     /locus_tag="AC16FV_04725"
     CDS             1890..2630
                     /locus_tag="AC16FV_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR2849404.1"
                     /translation="MLIPQLVAHRGYPRHYPENTLIGLEAAIAAGARFVEVDVQLSRD
                     RVPLLFHDRDLKRLCGVHGKVHDLRYEELWRLRVSERSRFGDRFKDVHITRLAELGHL
                     LLRRPEVTAVVELKRSSLERFGIETLLTLVRRSLKPALDQCILISYSLEALLTARNKG
                     WPRIGAVIDRWNEHKQELIAQIRPQFLFCDVEGLPREDRLQADDMKLAVFEVADPQVA
                     LALAARGVDFIETFAVGEMLQEFSRLAP"
     gene            2630..3799
                     /locus_tag="AC16FV_04730"
     CDS             2630..3799
                     /locus_tag="AC16FV_04730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR2849405.1"
                     /translation="MATPTYDVVIIGGGIHGVGVAQAAAATGHSALVLERQALGSGTS
                     SRSSKLIHGGLRYLESMQFGLVRESLREREILLRNAPELVRRVSFYIPVYSSTRRSPW
                     MIRAGLSLYALLGGLSRNARFESVPRARWEDLDGLDTHGLRTVFRYEDAQTDDVLLTQ
                     AVMRSAQALGAELRWPANFLSAIRCETGFRVHYLDRHGEQACHARTLVNAAGPWVNAV
                     LDRIAPRPPMLTVELVQGAHILLEGETRHGIYYVEAPSDGRAVFVMPWKGQTLVGTTE
                     TVFNGDPASVKALPQEISYLQETWRHYFPRVPGRLLDSFAGLRVLPQGPGSVFHRSRE
                     TVLYPDDTSQVRLVTVYGGKLTGYRAAAAKVMRLLRKSLLARPAVADTAQLKLSL"
     gene            3990..4343
                     /locus_tag="AC16FV_04735"
     CDS             3990..4343
                     /locus_tag="AC16FV_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR2849406.1"
                     /translation="MNIFVGNLSAAVTADDLRRLFAGYGTTINAIIMRDTDTGLPLGY
                     GHVYVVPEPAAYEAIINLDKITLKGSPITVRECVYRARQERRLRRFLWRGSERRATES
                     RRHNGHDQTSQHRLG"
     gene            complement(4350..5474)
                     /locus_tag="AC16FV_04740"
     CDS             complement(4350..5474)
                     /locus_tag="AC16FV_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR2849407.1"
                     /translation="MSRLSIFMMTLLMLVAADGVRAHEIRPAIATASFGAERYDIEIS
                     ANMEAVIAGVSPQHKDTNESPNAQTYNRLRELPPAELERKIREFQLQYLEGIEIRFDD
                     ARVQPALCALDVPGVGDPGRARLTRLHLCGAIPAGARIFRWAYAANFGESIVRLPAVS
                     GGDAVALWLKNGARSEPYILGVGMEQKTRLEVAAQYTVLGFTHILPKGLDHILFVLGL
                     FLLSVRWKPLLIQVTAFTLAHTLTLGLSIYGIVSLSPAIVEPLIAASIAYVAVENLIT
                     AELKPWRAFVVFGFGLLHGLGFAGVLQEIGLPRSEFLTALLTFNLGVELGQLTVITLA
                     YLLVGLWWKTKTWYRARLVQPLSALIAAVGVYWTIERIVG"
     gene            5582..>5932
                     /locus_tag="AC16FV_04745"
     CDS             5582..>5932
                     /locus_tag="AC16FV_04745"
                     /inference="COORDINATES: protein motif:HMM:NF022135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HIR2849408.1"
                     /translation="MRNSRCLLIAALSVMTWPAAAHDFWIEPQAFRPKPGTAVGVLLF
                     VGQDFKGESVPYFPQKFERYVIVGPTGTQPIPGVLGDEPAGTVTPATPGLYVVGLHTK
                     PDSVSFDTAEEFERY"
BASE COUNT         1149 a         1855 c         1792 g         1136 t
ORIGIN      
        1 gggatctcag aggccgccgc gcagcagcag ttcgcgcggg tcgggattca ggtaagtctg
       61 accggcaatg tacggatcag gatggcgccg gaagtggtgc ttgagcaaga tcagcggcgc
      121 ggcgaacggc gcctcgccct gacgataggc gtggatcatc ccgagcagtt cggccttgtc
      181 ctcggcgtcc agatattttt tcagataacc catcagatgc atcaatacat tggcgtggcg
      241 cgccggcgtt gccagcctct ggaacgcctg catcaggtgt aaaagatact cgcgtcctga
      301 ttctttcagg ttgcgccggc cggcctgcgc caccagtcgc ccgagcacac gataggcttc
      361 catgtcatgg gccatgagcg cgagcttgtg gcgcgtgtga aactccacca accgcgcggc
      421 ggtcagtccg tgagacgtgt attccctcca gcgccggtag acgaacacgc gctcgatgaa
      481 attttcccgc aggcgcggat caccgagccg gccctcctct tcggccggca gccagggctg
      541 accggcgaga aaggcatcgg cgtaaattcc ccggccgctc tttgccggca ccccggcatg
      601 ctgataaatc ttgacgcgct tcataccaca actcggcgaa cggctcttga acacatagcc
      661 actcagatcg tcgagcttgc gtgcctgttg tttcccgtag gcagcgagtt tgtccgtgac
      721 atcgatcgag gcgtccttca cgccaaccgc gcgcggcgcg gaggcgttgc cgaccagccg
      781 gattggcgcg cgcggcactc ccaggccgat agcgacttcc gggcagacgg gaacccactc
      841 gaaaaactgt ccgagcgtgc cggtcagaaa actgtcgagc ttgtggttgc catcaaagcg
      901 tactttctgt cccagcaggc aactgctgat gccgagacgg attttggcgc cgtggatgct
      961 cgttgttttg gtcactggtg tctgtcctgt ctgaacacgg acatcattct aaatcagccg
     1021 gtacgcttgc acggaactcc atgcaaaaaa agcacgccgg catgagccgg cgtgctttga
     1081 tgcgggttga ggaatgatca gaatcggaaa tacataccaa gcgacagaac cggataccat
     1141 ttcaaatcgc tcaggtcctc ttccgcgctg cggcgttcct gttcgacatc cgacgccaag
     1201 cccgcggcgg cgccggtagc cgacaaggcc agcttcggcg aaccctgata gagcacgccg
     1261 atatccgccg acaggcccaa cctgccaccc ggacgattgc cccagccgag gccgagataa
     1321 ggcgccgtgc tgttgaacgt gagctggccg gtcagtgtac ccacttgcgc agcggtatag
     1381 gtctgaccat tgataacgta gttagtaccg gtgggcttgc caatcaggtt caattcattt
     1441 ttgttgtaga ccgcaccacc ggtgaaatgg aaggcgccac cgaaaggatg gtaattggcg
     1501 aacaacgcca cggactgaag atcaaaattg aagtcgtaat caatgtcgct ggcactgtcg
     1561 gtagttttga agtcatagga attgaatccc agacccacgc tgaatttgtc gctgaaggac
     1621 ttggacaact ccacgccggc gcccaacgtt ccggccttga ggttgatgcc aacatcgcca
     1681 tgggcaacgt tcgtaaacca gggcaacatc aggacagcgc aaacggaaat gcaacggttt
     1741 ctcatggttt ctcctgtaat gtatctcctt cgaaacttag accatcccga tcctggcaca
     1801 agccgtcgcc atggctgatg ccggtaacgg ctgacagccg cacaggtctg aacggtacgc
     1861 ggtcatgccg gggtgctaaa gttgcgctta tgctcattcc gcagctggtg gcgcaccggg
     1921 gctatccccg gcattacccg gaaaacaccc tgatcgggct cgaggccgcc attgccgcgg
     1981 gcgcgcgctt tgtcgaggtg gatgtgcagc tttcccgtga tcgcgtgcca ttactgttcc
     2041 acgaccgcga tctgaagcgc ctgtgcggcg tgcacggcaa ggtccacgat ctgcgctatg
     2101 aggaactgtg gcgcctgcgc gtctccgagc gcagccgctt tggcgatcgc ttcaaagacg
     2161 tgcacatcac ccgcctggcc gagctcggcc acctgctgct gcgccggccg gaggtgaccg
     2221 ccgtcgtcga actcaagcgc tcgagcctcg agcgcttcgg catcgagacc ctgctcacgc
     2281 tggtgcggcg ttcgctgaaa ccggcgcttg atcagtgcat cctgatttcc tattcgctcg
     2341 aggcgctgct gacagcgcgc aacaagggct ggccccggat tggcgccgtc atcgaccgat
     2401 ggaacgagca caaacaggag ctgattgccc aaattcgtcc gcagtttctt ttctgcgacg
     2461 tggaagggtt gccacgtgaa gaccggcttc aggcggacga catgaagctc gccgtgtttg
     2521 aggtagcaga cccgcaggtg gcgctggcgc tggccgcgcg cggggtggat ttcatcgaaa
     2581 ccttcgcggt tggcgaaatg ctgcaggaat tctctcgcct cgcgccctga tggccacgcc
     2641 gacctacgac gttgtcatca tcggcggcgg cattcatggc gtcggcgtgg cccaggccgc
     2701 ggccgcgacg ggtcattcgg cgctggtgct ggagcgccag gcgctcggca gcggcacctc
     2761 cagccgctcc agcaagctta ttcatggcgg cctgcgttat ctggaaagca tgcagttcgg
     2821 acttgtgcgc gaatcgctgc gcgagcgtga aattctcctg cgcaacgcgc ccgagctggt
     2881 gcggcgcgtg tcattctaca ttccggttta ttcctccacc cgccgttcgc catggatgat
     2941 ccgcgccggg ctgtcgctgt atgccctgct cggcgggctt tcccgcaacg cgcgttttga
     3001 atccgtgccg cgggcgcgct gggaggacct cgacgggctg gacacccacg ggctgcggac
     3061 tgtgttccgg tatgaagacg cccagaccga tgacgtcctg ctgacgcagg ccgtgatgcg
     3121 ttcggcgcag gcactcggag cggaactgcg ctggccggcg aattttcttt cggcgatacg
     3181 gtgcgagacc ggcttccgtg tgcattatct cgaccgccat ggagaacaag cctgccacgc
     3241 ccggacactg gtgaacgccg ccgggccgtg ggtcaacgcg gtgcttgatc gcatcgcgcc
     3301 gcgcccgccg atgctgacgg tggagcttgt tcagggcgcg catatcctgc tcgaaggcga
     3361 aacccggcac ggcatctatt atgtcgaggc cccgagcgat ggacgcgccg tgttcgtcat
     3421 gccgtggaag gggcaaaccc tggttggcac cacggaaact gtttttaacg gcgatcccgc
     3481 aagtgtgaag gcactgccac aggaaatttc ctacctgcag gaaacctggc gacattattt
     3541 tccacgcgtc cctggccgtc tgctggacag tttcgccggc ctgcgcgtgc tgccgcaggg
     3601 accgggctcg gtatttcatc gttcgcgcga gaccgtgctg tacccggatg acaccagcca
     3661 ggtacgtctc gttactgttt acggcggcaa actcaccggc taccgcgcgg cggcggccaa
     3721 ggtcatgcgc ctgctccgga aatcgctcct cgcgcgcccc gcggtcgcgg acacggcgca
     3781 gttgaagctt tcgctgtgac gtcatgtcgt aacgacagcg tctggaatgt gtcttttttc
     3841 agctggcaaa ctgcgtgggg tttgtctcca aactccttgg cgcaaaagca cttttcgatt
     3901 tgaagatcga ggcggcttgt caggcgcgcg catcttccac aaaatgcgga aaaacataag
     3961 gccatcaggc caaacgcaag gggcggttaa tgaatatctt cgtgggcaat ctctccgcgg
     4021 cagtgacggc tgacgatttg cgccggctct ttgccggcta tggcaccacc atcaacgcca
     4081 ttatcatgcg cgacaccgac accggcctgc cactcggtta cggccacgta tacgtggtgc
     4141 cggaaccggc cgcctacgag gccatcatta acctcgacaa gataaccctg aagggcagcc
     4201 ccatcaccgt gcgcgaatgc gtctaccgcg cgcgtcagga acggcgcctg cgccgtttcc
     4261 tgtggcgtgg cagcgagcgg cgggcgacag aatcgcgccg tcacaatggc cacgatcaaa
     4321 cgtcgcaaca tcgtctcggc tgaatcggct cagccgacga tccgttcaat cgtccagtaa
     4381 actccgaccg cggcgatcag cgccgacaac ggctgcacca gacgtgcgcg gtaccaggtt
     4441 tttgttttcc accacaggcc gaccagcaga taggccaggg taatcacggt gagctggccc
     4501 agctccacgc cgaggttgaa cgtcaacagc gccgtcagga attccgagcg cggcagaccg
     4561 atctcctgca ataccccggc gaacccgagc ccgtgcagca ggccgaaacc gaagaccacg
     4621 aaggcacgcc acggtttcag ttccgccgta atcagatttt ccaccgccac gtacgcaatg
     4681 gaggccgcga tcaacggctc cacgatcgcg ggtgacagcg agacgatgcc ataaatactg
     4741 agcccgagcg tgagggtgtg cgccagcgtg aaagcagtca cctggatcag caacggtttc
     4801 caacggacgc tcagcagaaa aagcccgagc acgaacagga tgtgatccag tcccttgggc
     4861 aggatatggg tgaagccgag cacggtgtat tgcgcggcaa cttcaagccg cgttttctgc
     4921 tccatcccga cccccagaat gtacggctca ctcctggcgc cattcttcag ccacagcgcc
     4981 accgcatcgc cacccgacac agccggcagg cgcacgatgc tctcaccgaa gtttgccgca
     5041 taggcccagc ggaaaatgcg cgcgccggcg gggatcgcac cgcacaaatg caggcgcgtc
     5101 agccgcgcgc ggcccggatc gccaaccccg ggcacgtcga gcgcacacaa ggccggctgg
     5161 acgcgcgcat cgtcgaaacg gatctcgata ccttcgaggt actgtagctg gaactcacgg
     5221 attttccgct cgagctccgc gggcggcagt tcgcgcagac ggttgtatgt ctgcgcgttg
     5281 ggcgattcat tggtgtcctt gtgctgcggg ctcacacccg cgatcactgc ttccatgttg
     5341 gcgctgattt ctatgtcata acgctcggca ccgaagctcg cggttgcgat cgcgggacgg
     5401 atctcatgag cgcgcacgcc atcggcagcg accagcatca gcaaagtcat catgaaaatg
     5461 gataatcggg acatggtttg cgctttccgt tttgcggcga aaggtgtcgg gcttatgtta
     5521 tgctgccgat cgacactgct tcatggacct gcccgtgacg acaaaattcc gcgcgcagtt
     5581 tatgcgaaac tcacgatgtc tgctcatcgc cgcgttatcg gtcatgacct ggccggcggc
     5641 ggcccacgac ttctggatcg aaccgcaggc gttccggccc aagccgggaa cggcagtggg
     5701 agtgctcctg ttcgtgggcc aggatttcaa gggtgagtcc gtgccctatt ttccccagaa
     5761 attcgagcgc tacgtcatcg tgggcccgac cggcacgcaa ccgatcccgg gggtgctggg
     5821 cgacgaaccc gccggcacgg tgacgccggc tacgcccggg ctgtacgtcg tcggcctgca
     5881 caccaaaccg gactcggtca gcttcgacac cgccgaggaa ttcgaacgct ac
//
LOCUS       DBNGZE010000180         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1693649, whole genome shotgun sequence.
ACCESSION   DBNGZE010000180 DBNGZE010000000
VERSION     DBNGZE010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1693649"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="AC16FV_04750"
     CDS             complement(<1..716)
                     /locus_tag="AC16FV_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR2849409.1"
                     /translation="MTVLRFGVTPKMKPVSPKRAAVVAGLDIGTSKVVCLVARLEPQA
                     PQDVLRRRSHGVRLLAFAHTAASGMKAGSVVDLVEAEDVVRQAIDIAESIAGVQLESV
                     VVSMSGGRLGSERFIANIDLAGRAVTEADISRVLAAASRHSVRDGRAVLHSLPIGYAV
                     DAAAGIREPRGMLGHRLGVDMHMVTADVATVRNLMLAVERSHLSVKAMVASPYVAGLA
                     ALADDEADLGAATVDMGAGTT"
     gene            complement(713..1663)
                     /locus_tag="AC16FV_04755"
     CDS             complement(713..1663)
                     /locus_tag="AC16FV_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR2849410.1"
                     /translation="MEGRVRLAQSLKLKRRSSTVLWPKSHSRHWLRLVRRWSVLLVGL
                     KVPRGAGSSAAAMLLLASVSYGAVKGGHVPQIIENAQDLCDAAANRLGFRIFEVALDG
                     EHEVARDEIQILAGITDRSSLLFFDAARARARLLANPWIADATVLKLYPDRLRIEIKE
                     RKPFALWQKDGRVSLVAADGTVLEATVPPRFNGLPRIIGKGAGQSARDFLALLARYPM
                     IAQMTEVSVLVAERRWNLHLKGGLEILLPEGETEQALRILIDLDRSDKLLSRDIVAVD
                     LRLPDRVTVRQSDSAAAAREQVLKDAEKTKKGKAGKGGEA"
BASE COUNT          356 a          579 c          520 g          333 t
ORIGIN      
        1 gtggttccgg cgcccatatc gaccgtagcc gcccccagat cagcctcgtc atcggcgagg
       61 gcggcgagac ccgccacata gggggaggcc accattgcct tcacagaaag atggctgcgc
      121 tcgaccgcaa gcatgaggtt ccgtacggtc gccacgtcgg cggtgaccat gtgcatatcc
      181 acacccagac ggtggccaag catgccacgc ggttcgcgga taccagcagc agcatcgacc
      241 gcgtagccga tcgggaggga atgcaggacg gcgcgtccat ctcgcaccga atgtcggctc
      301 gccgctgcga gtacccgact aatatcggct tcggtcactg cgcgaccagc gagatcgata
      361 ttcgcgatga agcgttcgct gccaaggcgg ccgccggaca tcgacaccac gacggactcg
      421 agttgaacgc ctgctatgct ttcagcaatg tcgatcgcct ggcggaccac gtcctccgcc
      481 tcgaccagat cgaccacaga accggccttc atgccgctcg ccgcggtatg ggcaaaggcc
      541 aggagccgta cgccgtggct gcgccggcgc aggacatctt gtggcgcttg cggttcaagc
      601 cgcgcgacga ggcagacgac cttgctagtg ccaatatcga gaccagcgac gacagctgcg
      661 cgcttgggcg aaaccggctt cattttcggg gtaacaccga agcgcagcac ggtcatgctt
      721 cgcctccttt gcccgccttg cccttttttg ttttctcggc gtccttcagc acctgctcgc
      781 gcgcagcggc agcgctgtca gactggcgca cggtgacacg gtcgggcagc ctgagatcga
      841 ccgccacgat gtcccgcgac agcagcttat cgctgcggtc gagatcgata agtatcctta
      901 gcgcctgctc ggtttcgcct tccggcaaca gaatctcgag gcctccttta aggtgcagat
      961 tccaacgccg ctcggccacc agaaccgaga cttcggtcat ctgcgcgatc atcggatatc
     1021 gcgcgaggag cgcgagaaaa tcccgcgcgc tctgcccagc acccttgcct atgatgcgtg
     1081 gcaatccgtt aaaacgcggc ggcacggttg cttcgagcac ggtaccatcg gccgcgacca
     1141 gcgaaacacg accgtccttt tgccagagcg caaatggttt gcgttctttg atctcgatgc
     1201 gtagccgatc aggatatagt ttgaggacgg ttgcatctgc aatccacgga ttggccaaca
     1261 agcgcgcacg tgcgcgcgca gcatcaaaaa acaacagcga cgagcggtca gtgatgcccg
     1321 cgagtatctg aatttcgtcc cgggcaactt cgtgttcacc gtcgagtgcg acctcgaaaa
     1381 tacgaaagcc gagtctattg gccgctgcgt cgcagagatc ttgcgcattt tcgatgatct
     1441 gcgggacatg gccgcccttg actgcgccgt agctgacgct ggcaagcagt aacatggcgg
     1501 ctgccgatga gcctgcgccg cgcggcactt tcaagccgac aagcaataca ctccagcggc
     1561 gcacaagcct cagccagtgc cgactatgcg acttcggcca caaaacggtc gatgagcgtc
     1621 gtttcaactt gagcgattga gcgaggcgca ctcgaccttc cattgcgacc tactttactt
     1681 gttgagccca acgtggggcc gcaagctccg ccatgagcat gacgtcgtca tgctccgata
     1741 tccggcttca gatgagacca tccccgtccc tcggaggccg ccgtcagc
//
LOCUS       DBNGZE010000181         2861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1554543, whole genome shotgun sequence.
ACCESSION   DBNGZE010000181 DBNGZE010000000
VERSION     DBNGZE010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1554543"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="AC16FV_04760"
     CDS             complement(<1..1268)
                     /locus_tag="AC16FV_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR2849411.1"
                     /translation="MFVVLLLATAGCATTSPETAQTAKDSASDAAVPLVSKDNSSAPA
                     AAVTSKPESSKPHATFDEQAASNHSDIWARIRAGLALEPLDSPLVDAEARWFANNPEY
                     MQRMMDRAKLYLYYIVEEVEKRGMPMEIALLPAIESAYKPLAYSRARASGLWQFIPST
                     GRLYGLKANWWYDGRRDVQASTQAALDYLEKLYNDFDGDWHLAIAAYNAGEGKVMRMM
                     EYNRRKGKPTEFQYLKLKRETQHYVPKLLAMASIVADPDKHGVKLADIPNEPYFARVE
                     TGSQVDLGVVAKLVDLPVDDLHMINPGYRRWATDPNGPHQLLVPADKKDVLIEGLNNL
                     PEEQRIKWQHHEVKRGETLHEVGRRYGLDVATLRTANNLRSNLLRVGQDLLIPISARP
                     LTVAAVRTTYRSSDARIAGRSEPVIHRVRRG"
     gene            complement(1434..2210)
                     /gene="gloB"
                     /locus_tag="AC16FV_04765"
     CDS             complement(1434..2210)
                     /gene="gloB"
                     /locus_tag="AC16FV_04765"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR2849412.1"
                     /translation="MTDVLHVPAFEDNYIWLIRGKSPERVAVVDPGDADPVLAALEKQ
                     RLTPVAILCTHHHGDHVGGVEDILKHYSIPVYGPARERIPTLTHRLKDGDHINIAGLD
                     LEFDVLDVPGHTAGHIAYVGGGMLFCGDTLFSAGCGRLFEGTAEQMHTSLSRFAALPE
                     ATRVYCGHEYTEANLRFAAAVEPDNADSLAYRERAQALRQQNLPTLSSTIGLERRVNP
                     FLRSGQPGVRRAAEKQAGRALVSEVEVFAAVRRWKDGFRG"
     gene            2245..>2861
                     /locus_tag="AC16FV_04770"
     CDS             2245..>2861
                     /locus_tag="AC16FV_04770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2849413.1"
                     /translation="MSERDSCSMQRSQLRQWFDSPLGLSLQAHEAHHLRVVLPNLYGT
                     VAAQLGRIGDLDLMDACIAPTRILLDDQVAPGACRVRSVPEELPLDTKSTDVVLLPHT
                     LDFCDDPHQVLREVSRVLRPEGHVVILGFNPMSLWGFRRLFTRRPRPAPWCGKFFRLS
                     RIKDWLGLLEFETTHGMMLYYRPPLRNEGFMQRLHFLDRMGDRWWP"
BASE COUNT          489 a          864 c          928 g          580 t
ORIGIN      
        1 ccacggcgta cgcggtggat gacgggttcg ctgcgaccgg cgatgcgcgc atccgatgag
       61 cgataagtgg tgcgaaccgc cgccaccgtg agcgggcggg cggaaatcgg tatcagcagg
      121 tcctgtccca cgcgcagcag gttgctgcgc aggttgttgg cggtgcgcag cgtcgcgacg
      181 tcgagtccgt aacgccggcc gacctcatgc agggtctcgc cacgtttgac ctcatgatgc
      241 tgccacttga ttctttgctc ttccggcagg ttgttcaggc cctcgatcag cacatccttc
      301 ttgtcggctg gcaccagcag ctggtgcgga ccgttcgggt cggtggccca gcgccgataa
      361 ccggggttga tcatgtgcag gtcgtccacc ggcaggtcca cgagcttcgc caccacgccg
      421 aggtccactt gggagccggt ttcaacgcgc gcgaagtaag gctcgttggg gatgtcagcg
      481 agcttgacgc catgtttgtc cggatcggcc acgatgctgg ccatggccag gagcttgggc
      541 acgtaatgct gcgtttcgcg cttgagcttg agatactgga attcggtcgg tttgcccttg
      601 cgacggttgt attccatcat gcgcatgacc ttgccctcgc cggcgttgta ggccgcgatc
      661 gcgaggtgcc agtcaccgtc aaaatcgttg tagagttttt cgagataatc cagcgctgcc
      721 tgcgtggagg cctgcacgtc gcgccggccg tcgtaccacc agttggcctt caagccatac
      781 aggcgacccg tggacggaat gaactgccac aggccggagg cgcgggcgcg cgagtaggcg
      841 agcggcttgt aggcgctttc gatggcgggc aacagcgcga tttccatcgg catgccgcgc
      901 ttttccactt cctcgacgat gtaatagaga taaagcttgg cgcggtccat catgcgctgc
      961 atgtattcgg gattgttggc gaaccagcgc gcctcggcat ccaccagcgg actgtcgagc
     1021 ggctcgaggg caagtccggc gcggattctg gcccagatgt cggaatgatt gctggccgcc
     1081 tgctcatcga aggtagcgtg cggtttgctg ctttccggct tggaggtgac ggccgcggca
     1141 ggcgcggagg aattgtcttt ggaaactaat ggaacagccg catccgatgc cgaatccttg
     1201 gcagtctggg cggtctccgg ggaagttgtt gcacatccgg cggtggccag gagcagaaca
     1261 acgaacaaga cctgattctt aattcttttc caatccatct ggggcaatag tatttgttgg
     1321 aggaatgagc gtcaacttgt tgaactgcat agttaagtaa atgccaatat cgcgatttgt
     1381 ccagtgcgag ggtaatcgga ttcggccctt ttgtccgaaa ccttgcccgc cgctcatcct
     1441 ctgaatccat ccttccatcg gcgcaccgcc gcgaacactt ccacctccga aaccagggcg
     1501 cgacccgcct gtttttccgc ggcgcggcgt actccgggct gcccggagcg caaaaatggg
     1561 tttacgcgcc gctccaggcc gatggtggag gacaaagtgg ggaggttttg ctggcgtagc
     1621 gcctgtgccc gctcgcggta ggccaggctg tcggcgttgt ccggttcgac cgccgccgca
     1681 aaacgcaggt tggcctcggt gtattcatgc ccgcagtaaa cccgtgtggc ttcgggcagc
     1741 gcggcgaagc gtgacagtga cgtatgcatc tgctccgccg tgccctcgaa cagccgcccg
     1801 caaccggcgg agaacagggt gtcgccgcag aacagcatgc cgccgccgac ataggcgatg
     1861 tgcccggcgg tgtggcccgg gacgtcgagc acgtcgaact ccaagtcgag tccggcaata
     1921 ttaatatgat cgccgtcttt gagccggtgc gtgagggtcg ggatgcgctc gcgcgccggg
     1981 ccgtacaccg gaatggaata atgtttgaga atatcctcca cgccgcccac gtgatcgccg
     2041 tggtgatggg tgcagaggat ggcaacgggc gtgaggcgct gcttttccag cgccgccagc
     2101 accggatcgg cgtcgccggg gtcgacgacg gccacccgct ccggggattt cccgcggatg
     2161 agccagatat agttgtcctc gaaggcaggg acgtgtaaaa catcggtcat gcatccagtt
     2221 tgagatgccg caggagggac gtcaatgagc gaacgtgatt cctgttccat gcagcgcagc
     2281 cagttgcgcc agtggttcga ttcgccgctc ggcctgtcgt tgcaggcgca tgaggcgcat
     2341 catctgcgcg tggtgctgcc gaacctgtac ggcaccgtgg cggcccagct cgggcgcatc
     2401 ggcgatctcg atctcatgga tgcctgcatc gcgcccacgc gcatcctgct tgatgatcag
     2461 gtcgcacccg gcgcttgccg ggtgcgcagc gtcccggaag aactcccgct cgacaccaag
     2521 agcaccgacg tggtgctgct gccgcacacc cttgatttct gtgatgaccc gcaccaagtg
     2581 ttgcgcgagg tcagccgcgt gctgcgtccg gaagggcatg tcgtgattct cggtttcaat
     2641 cccatgagcc tgtggggctt ccgccgcctg ttcacgcgcc ggccgcgccc cgcgccgtgg
     2701 tgcggaaaat tcttccgcct gtcgcgcatc aaggactggc tcgggctgct cgagttcgag
     2761 accacgcacg gcatgatgct ctattaccgt ccgccgctgc gcaacgaagg cttcatgcag
     2821 cggctgcact tcctcgacag gatgggcgac cgctggtggc c
//
LOCUS       DBNGZE010000182         9562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_650853, whole genome shotgun sequence.
ACCESSION   DBNGZE010000182 DBNGZE010000000
VERSION     DBNGZE010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_650853"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..266
                     /locus_tag="AC16FV_04775"
     CDS             24..266
                     /locus_tag="AC16FV_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024783.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2849414.1"
                     /translation="MDRPAIPAEIRRAVLVEAGHRCAIPTCKHPTTDIHHIVPWESCK
                     SHDFDNLIALCPNCHNNWGQITVFAKINRNRDLTTA"
     gene            301..714
                     /locus_tag="AC16FV_04780"
     CDS             301..714
                     /locus_tag="AC16FV_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849415.1"
                     /translation="MSDTPKYPWLIDSNERYLEVVRTLIGLSTASLVIPVFLMRDVFG
                     VEPTKSIMNFFTCSVYASWISFGIAILSGIIFHYFSAKWIRMAWDQPAWFIGKTLSKK
                     KVEMMLDLAFWISAAGFMFGLGFIIYFFASYVHAS"
     gene            complement(1155..1547)
                     /locus_tag="AC16FV_04785"
     CDS             complement(1155..1547)
                     /locus_tag="AC16FV_04785"
                     /inference="COORDINATES: protein motif:HMM:NF017660.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IceA2 protein"
                     /protein_id="HIR2849416.1"
                     /translation="MRTTWNLPDCCHENFIGRHRHRRRGIRLQHEQRRAVMGLELKIV
                     NGKIEEYEDGRCRRSYGSNVADAHTDGGIVAVVTADGRVEEYRDGMCQRSYCTNAQKV
                     RVSGSTLAVTLQDGRVAEFENGSCRRMY"
     gene            1799..4057
                     /locus_tag="AC16FV_04790"
     CDS             1799..4057
                     /locus_tag="AC16FV_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR2849417.1"
                     /translation="MPSARAIFSYRKFWAKRFGPAPFLPMSRAEMDELGWDSCDIILV
                     TGDAYVDLPSFGMAIVGRVLEAQGFRVGIIAQPDWRNAEAFKALGRPNLFFGISAGNM
                     DSMVNRYTADRKIRSDDAYTPGGAAGRRPDRSVIVYTQRAREAFGDVPIIIGGIEASL
                     RRIAHYDYWSDKVRRSILLDAKADLLVYGNAERQVVEIAHRLAVKEPIQNITDIRGTA
                     FVRNSLPEGWTELDSTHLDTPGALNPPVNPYASEEEMRATNSIRGELVEPRTDIRTST
                     GSVRTDEGLRVNVVQLHKRATKLDREKTVVRLPAFEAVANDHILYAHASRVMHVETNP
                     GNARALVQRHGDRDVWLNPPPLPLTTKEMDGIYELPYQRQPHPAYGKEKIPAYEMIRF
                     SIAIQRGCFGGCTFCSITEHEGRIIQNRSEESIIREIETIRDTVPGFTGVISDLGGPT
                     ANMYRLHCKSKEIESACRKPSCVYPDICQNLITDHAPLIHLYKRARELPGIKKVLIGS
                     GVRYDLAIQSPAYVKELVQHHVGGYLKIAPEHSREGPLSKMMKPGMGAYYKFKELFDK
                     YSREAGKEQYLIPYFIAAHPGTTDEDMLELALWLKQNGFRADQVQAFLPSPMATATAM
                     YHSGKNPLRKVTRGSEPVYIPKGLKQRRLHKAFLRYHDANNWPMLRAALRRMGRSDLI
                     GSGKHQLIPTYQPAGTGQVHEGHRGGKRFATQHTGLPKFTPKKSGQRSHHGQRPGKTL
                     HPGSPTGAGKRR"
     gene            4236..5417
                     /gene="dinB"
                     /locus_tag="AC16FV_04795"
     CDS             4236..5417
                     /gene="dinB"
                     /locus_tag="AC16FV_04795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR2849418.1"
                     /translation="MILHIDMDAFYASIEERDRPGLRDKPMVVGGNPDKRGVVAAANY
                     IARRYGIHSAMPTSQAKHLCPNLIVLPPRHSYYAQVSQQIRDIFHRYTPLVEPLSLDE
                     AFLDVKHSHLLFGDATEIGRRIKREIRDELKLTASVGIAQNKFLAKVASEAGKPDGFL
                     EVNPAGVQAFLDTLPVSKLWGVGKVGERSLRKHGIRTIGELRAGPRALLDELFGSWGG
                     TLWNLAHGIDTRTVIPDHKAKSLSHETTFAEDITDVEALRGWLLDLTRQVAMRLRCNH
                     LRGRIVHVKIRFADFRTITRSRTLPQSTDVTQELWDTGLTLLTQNLPLRHRGVRLLGF
                     SVSGFDSDESRQADLFSESERAKNVRLDTVSDWIQARFGAAALTRATELVSTALVSTA
                     E"
     gene            complement(5434..5631)
                     /locus_tag="AC16FV_04800"
     CDS             complement(5434..5631)
                     /locus_tag="AC16FV_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR2849419.1"
                     /translation="MARKYIDCREYPSEMNCTIAIFADSDKELLEAAVQHAVAVHGHK
                     DDPELREQLQKMFKEEHRRAA"
     gene            complement(5890..6087)
                     /locus_tag="AC16FV_04805"
     CDS             complement(5890..6087)
                     /locus_tag="AC16FV_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR2849420.1"
                     /translation="MIRKFIDCRDFPNEKNCTVAIFADNEHELLEAAVQHAVIAHGYE
                     DTPELRAQLLQASKIERRRAA"
     gene            complement(6351..6551)
                     /locus_tag="AC16FV_04810"
     CDS             complement(6351..6551)
                     /locus_tag="AC16FV_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR2849421.1"
                     /translation="MVRKFIDCRDVPGVKCTVAIFAENEEELLEAAIQHAVGAHGCED
                     TPELRERLRQHFKIEKRRAICG"
     gene            complement(6697..7950)
                     /locus_tag="AC16FV_04815"
     CDS             complement(6697..7950)
                     /locus_tag="AC16FV_04815"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HIR2849422.1"
                     /translation="MKVLVLGSGVIGVTSAWYLRRAGHEVTVVDRQAAPGLETSQANG
                     GQVSWGAGTPWATPGIPLKALKWMLRPHSPLVLRPRLDPVMWKWLFSMLANCTAERYA
                     VNRERMLRLSRYSHECLKALRRETGIRYDEHTTGVLELFRTAHELDDAVRDAALLKQW
                     GVDCRLLDRAGCMAQEPALRVSQEKIAGGLHFPGDESGDCFQFTQSLAKLAESQGVSF
                     ALNTRIERLEADGDRITRVHTDKGALAADACVLACGSYSPLLVRPLGIRLPVYPVKGY
                     SVTLPLTDAKAAPAGSVTDVTYKVVITRLGDRLRGAGTAELAGYDMSLRPSRLATISH
                     VLADLFPGAGDPARAQPWCGLRPMTPDNPPVLGVSRYKNLYLNTGHGTLGWTMACGSG
                     KILADIVSGRPADIDLNGLDLARFS"
     gene            8223..9065
                     /locus_tag="AC16FV_04820"
     CDS             8223..9065
                     /locus_tag="AC16FV_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2849423.1"
                     /translation="MSMIKHVIRFIQWAAVSLLLGVAVTQGAIEKSTAEFPYRSQYDV
                     NIIETAEFSVKLSAAVAVDVRTKYEYETLHIKGAINIPLDKNFIDNVRKLRAKDTRPI
                     IFYCNGKTCRKSYDAATMAGKAGISDVYAYDAGILVWAETHPENTVLRGKASIQRSDL
                     IGEKDFASYVLAPKAFATLFEQLGDKAMVLDIRDRVQRDNPVFGLVEERIPLDDAARL
                     DAFIETVKKHRKTLLVYDKAGHQIRWFQYYLREKGVRDYRFLKGGSEGYFVETLGLKE
                     FGKK"
     gene            complement(9212..>9562)
                     /locus_tag="AC16FV_04825"
     CDS             complement(9212..>9562)
                     /locus_tag="AC16FV_04825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR2849424.1"
                     /translation="RDDKDARLKAWNNNYSFFGAPVGLLFFLDRALEKGSWVDMGMFI
                     ENVMLSALGHGLATCPQASIAEYPDIVRKLVNVPDTRALLCGMALGYPDAGAPVNNYR
                     TAREPVASFTAWLT"
BASE COUNT         2018 a         2800 c         2782 g         1962 t
ORIGIN      
        1 ggttttttca ggctgaccac gctatggata gaccagcaat ccctgctgaa attcgtcgcg
       61 ccgttctggt tgaggcagga catcgctgcg cgattccaac gtgcaaacac ccaacgactg
      121 atattcatca cattgtcccg tgggaatcat gcaaatcaca cgatttcgat aatctgattg
      181 cgctttgccc aaactgccat aacaactggg gtcagatcac agttttcgct aaaattaacc
      241 gaaaccgtga tttgaccaca gcttagttca tgcaaaatgt aggaaggagg agtccatccc
      301 atgagtgaca caccaaagta tccatggctg atagatagta atgaacgcta tctggaggta
      361 gtaagaactc ttattggctt gtcgacggcg tcactagtga tccccgtgtt cctcatgagg
      421 gacgtatttg gcgtcgagcc aaccaagtcg atcatgaact tttttacctg cagtgtttat
      481 gcatcgtgga tctcgtttgg aatagcgata ctttcaggga tcatctttca ttacttttct
      541 gccaaatgga ttcgaatggc gtgggatcaa cctgcatggt tcatcggcaa aacactatcc
      601 aagaaaaaag ttgaaatgat gcttgatttg gcattctgga tttcagctgc tgggtttatg
      661 tttggtttag gcttcataat ctacttcttt gccagctatg ttcatgcttc ttagcttgct
      721 gctcggaatt taagaaaaaa gaagatgcct caggctcaat tattcaataa accagtggaa
      781 aggaaatggc aacatatcga gagattcagc aggacatcaa aaagcgacac gggcgcacag
      841 tgaaaacttg ctggattgca catgtgaaag agctaaatgg cttacgtccg aggatagcgc
      901 caaacagaca ttcgaagaca aggcgcgagg taccgtgtcc tcccgaaatg cgtccactca
      961 ttgaagcttc gatgcgtcgg ctaggtttgc tctcataaga ggtagtcctt tcaagccaac
     1021 cgtggaattc agcccagttc acgggtagac tgcgcctcct gccttgatac ggcgttccgg
     1081 cgccacctca gttcccatgc aaaccgcgcg tcggcttgat ttcccgggtc gctaaaccgg
     1141 tcgccaatcg ttttttaata cattcgccgg cacgacccgt tctcgaattc tgcgacccgc
     1201 ccatcctgaa gcgtcacggc taaggtgctc cccgaaaccc ggaccttctg ggcgttggta
     1261 cagtaggaac gttggcacat gccatctcga tattcctcga cccgaccgtc tgccgtaacc
     1321 acggcaacaa tgccgccatc ggtatgcgcg tccgcaacgt tcgacccata agagcgtcga
     1381 catctgccgt cctcatactc ttcgatcttg ccattgacta tcttaagctc aagccccatc
     1441 actgctctcc tttgttcatg ctgcaaccga atgccgcgcc tgcggtggcg atgacggcca
     1501 ataaaattct catggcaaca atctggcaag ttccaggttg tgcgcattgc atggaatttg
     1561 attcaagctg gttttcgcga gcgccggttg ttgcaggttg cggatcaaca gcaaacatca
     1621 ccatggatac cttctcattg acctacgtca agagttcggc atagcgggtg acgtcgaagt
     1681 gccaaaaaat cggaataatt ggggacttac cacgcagcgc tgtggaattc gccccatttc
     1741 ataggtagac tgcgcctcct gccttgatac ggcggtactg cgccgccatc tctttcccat
     1801 gccatccgcg cgtgctattt tttcctaccg caagttctgg gcgaaacgct tcggtccggc
     1861 gccgttcctg cccatgtcgc gcgcggagat ggacgagctc ggctgggatt cctgcgacat
     1921 catattggtt accggcgacg cctacgtgga cctgccgagc ttcggcatgg ccatcgtcgg
     1981 gcgcgtgctg gaggcgcaag gctttcgcgt cgggattatc gcgcaaccgg attggcgcaa
     2041 cgccgaggcg tttaaggcct tgggccggcc gaatctgttc ttcggcatca gcgccggcaa
     2101 catggattcc atggtcaacc gctacaccgc cgaccgcaaa atccgctcgg acgacgctta
     2161 tacccccggc ggggcggcgg gtcggcgccc ggaccgctcg gtgatcgtgt acacccagcg
     2221 ggcgcgtgag gccttcggag acgtacctat tattatcggc ggcatcgagg cctcgttgcg
     2281 gcgcatcgcg cattacgatt actggtccga caaggtgcgc cgctcgatcc tgctggatgc
     2341 caaggccgac ctgctggtct acggcaatgc tgagcgccag gtggtcgaaa tcgcgcatcg
     2401 cctcgcggtc aaagagccga ttcaaaatat caccgacatc cgcggcaccg ccttcgtgcg
     2461 caacagtttg ccggaaggct ggaccgagct ggattccacg catctggata cgcccggcgc
     2521 gctgaatccg ccggtgaatc cttatgcgag tgaggaagag atgcgggcaa ctaattccat
     2581 tcgtggtgag cttgtcgaac cacgaactga cattcgcact tcgacaggct cagtgcgaac
     2641 ggatgaaggg cttagggtga acgtggtgca gttgcacaag cgcgcaacga aattggaccg
     2701 cgagaaaacc gtggttcgac tgccggcgtt cgaagcggtg gccaacgatc acatcctgta
     2761 tgcccacgcc tcgcgcgtga tgcatgtcga aaccaatccc ggcaacgcgc gcgcgctggt
     2821 gcagcgccac ggcgaccgcg acgtgtggct caacccgccg ccgttgccgc tcacgacgaa
     2881 agaaatggac ggcatctacg agctgccgta ccagcgccag ccgcacccgg cctacggcaa
     2941 ggaaaagatc ccggcctacg agatgatccg tttttccatc gcgatccagc gcggctgttt
     3001 cggcggctgc accttctgct ccatcaccga gcacgagggg cgcatcatcc agaaccgctc
     3061 ggaggagtcg atcatccgcg agatcgaaac catccgcgac accgtgcccg gcttcaccgg
     3121 cgtgatctcc gacctcggcg ggccgacggc caacatgtac cggctgcatt gcaagagcaa
     3181 ggagatcgaa tccgcctgcc gcaagccctc gtgcgtgtac cccgacatct gccagaacct
     3241 gatcaccgat cacgcgccgc tgattcattt gtacaagcgc gcacgcgagc tgccgggcat
     3301 caagaaggtc ttgatcggct ccggcgtgcg ctacgacctc gccatccagt cgcccgcgta
     3361 tgtcaaagag ctcgtacaac atcacgtcgg cggctatctc aagatcgcgc ccgaacattc
     3421 acgcgagggg ccgctgtcca agatgatgaa gccggggatg ggggcgtact acaagttcaa
     3481 ggagctgttc gacaaatatt cccgggaggc aggcaaagag cagtatctga ttccctattt
     3541 catcgcggcg catccgggta ccaccgacga ggacatgctg gaactcgccc tgtggctgaa
     3601 gcagaacggc ttccgcgccg accaggtgca ggccttcctg ccgagcccga tggcgaccgc
     3661 cacggcgatg taccactccg gcaagaaccc actgcgcaag gtgacccgtg gaagcgagcc
     3721 ggtgtacatc ccgaaggggc tgaagcagcg ccgcctgcac aaggccttcc tgcgctatca
     3781 cgacgctaac aactggccga tgctgcgcgc tgcgctgcgg cgcatgggcc ggtccgacct
     3841 cattggctcg ggcaagcacc agctcatccc gacctatcag cctgccggga ccggccaggt
     3901 gcacgaggga catcgaggcg gcaaacgctt tgccacgcag cacaccggct tgccgaaatt
     3961 caccccgaag aaatccggcc agcgctcgca tcacggtcag cgacccggca agaccttgca
     4021 tcccggcagc cccaccggcg ccggcaagcg gcgctgaatg aagcccgttc gtggtgagct
     4081 ggtcgaacca tgaacgaaca atttagcctg ctctgaatat gtcgcacttc gacaggctca
     4141 gtgcgaacgg agcttaggca tacatttcat tgcgtgctcg tgttacgatg cacgtccaac
     4201 atgacccgct attcccgcag gctgaatcac aagagatgat cctgcacatc gacatggatg
     4261 cgttctacgc ctccatcgaa gagcgggatc gcccgggcct tcgcgacaag ccgatggtcg
     4321 tcggcggcaa tcccgacaag cgcggggtgg tggcggcggc gaattacatc gcgcggcgtt
     4381 acggcatcca cagcgccatg ccgacgtcac aggccaagca tctttgtccc aatctgattg
     4441 tcctgccgcc gcggcattcc tattacgccc aggtctcgca gcagatccgc gacatttttc
     4501 atcgctacac gccgctggtc gagccgctgt cgctggatga ggcgtttctg gatgtaaaac
     4561 acagccatct gctgttcggc gacgccactg aaatcggccg gcgcatcaag cgcgagatcc
     4621 gggacgaact gaaactcacc gcgtcggtgg gtatcgcgca aaacaagttt cttgccaagg
     4681 tggccagcga ggcagggaag ccggacggtt ttctggaagt gaatcccgcc ggcgtgcagg
     4741 ccttcctcga tacgctcccg gtgtcgaaac tctggggcgt gggcaaggtc ggcgaacgga
     4801 gtttgcggaa acacggcatc cgcaccatcg gcgaattacg tgcgggcccg cgagcgctgc
     4861 tggatgagtt gttcggcagc tggggtggta cgctctggaa tctggcgcac gggatcgaca
     4921 cgcgtactgt gattccggac cacaaggcaa aatccctctc gcacgaaacc acgttcgcgg
     4981 aggacatcac cgatgttgag gcgttgcgcg gctggctgct cgatctgacg cgtcaggtgg
     5041 ccatgcgcct gcggtgcaac catctgcgcg ggcggatcgt gcacgtgaaa atccgcttcg
     5101 ccgatttccg caccatcacg cgctcgcgca cgttgccaca gtccacggac gttacgcagg
     5161 agctgtggga caccggcctg acgctgctga cgcagaatct ccctctgcgg catcgcggcg
     5221 tgcgcctgct cgggttcagc gtgagcggat tcgacagcga cgagtcgcgg caggcggatc
     5281 tgttcagcga atcggagcgg gccaagaacg tccggctcga taccgtcagc gactggatac
     5341 aggcgcgctt cggcgcggcg gcgctgacac gcgccaccga actggtttct accgcactgg
     5401 tttctaccgc ggaataaccc tgtcgcgcgc ggctcaggcg gcgcgccgat gctcttcctt
     5461 gaacatcttt tgaagctgtt cgcgcagttc cgggtcgtcc ttgtgcccgt gcacggctac
     5521 ggcgtgttgc accgccgctt ccagcagttc cttgtcgctg tcggcaaata tggcaatggt
     5581 gcaattcatc tcgctcggat attcacggca atcgatgtat ttcctggcca tggctgtctc
     5641 cttgctggtt cagaaaagat gcttttgtga gggaggttac ggctagggac ccggaaaacg
     5701 gatgaatggg tccgaaatca cgctatacct ttccctccgg aggtgtcaag gaagtctcga
     5761 aggcagcggg tttttgaggc gtgaatggtg gtcgctggac tcttgttcag gcgcaaaaga
     5821 aacacgccag ccccgccaga ccaggccggc acacgagacg actgtattac gccaaaacgc
     5881 tgaggaaaat caggccgcgc ggcgtctttc tatcttggat gcctgtaaca attgagcgcg
     5941 cagctccggc gtatcttcgt acccatgagc gatgaccgcg tgctgcaccg cagcttccaa
     6001 caactcgtgc tcgttgtcgg cgaagatcgc caccgtgcag tttttttcgt ttggaaaatc
     6061 ccgacagtct atgaacttcc tgatcatggc tcctccattt tattgtttcg agtataactt
     6121 tcggaaaaga ccagctaatt caggaccccc acatccaatt cgtgagtcct gtcccaagct
     6181 taatcctaga gggaagtgtg tcaagcaata cagctttgta acttttgttt cgttctgcga
     6241 cggaaggaca cggagggggt caacaaactg tcagaataat cgacacagca tgatgtcttc
     6301 aggtcaaaat gcgtgccaac ggcccggaca ccgctgtcga ggacaggtga tcagccgcag
     6361 atggcgcggc gtttctcgat tttgaaatgt tgccgcaagc gttcccgcag ttcgggcgtg
     6421 tcctcgcacc cgtgagcgcc taccgcgtgc tggatcgcag cctccagcag ctcctcctcg
     6481 ttttcggcaa agatggccac cgtgcatttg acgcccggaa catcgcgaca atcaatgaat
     6541 tttctgacca tgacccccag acccctgaat gaaaccggtg taagtccggt gccggaccaa
     6601 agccgctgga caaaaggctt atgtgtggca tgcctcaagt ccgggttgcc ccggcatcag
     6661 caacatagac gcggggaggg ttagtctcat tccatttcag gagaagcggg cgagatcgag
     6721 accgttcaga tcaatatcgg cggggcgacc cgaaacaata tccgccagga tttttcccga
     6781 gccgcaggcc atggtccagc cgagcgtgcc gtggccggtg ttgaggtaga gatttttgta
     6841 tcgtgaaacg cccagcaccg gcgggttatc cggtgtcatc ggtcgcagcc cgcaccaggg
     6901 ctgtgcccgc gccggatcgc cggcgcccgg aaagagatcg gccagcacat gcgaaatggt
     6961 ggcgagccgt gagggccgca acgacatatc gtagcccgcc agttccgccg tccccgcgcc
     7021 gcgcagcctg tcgccgagac gcgtgatgac gaccttgtat gtaacgtcag tcacactgcc
     7081 ggccggagcc gctttggcat ccgtgagcgg aagggtgacg gaataacctt tgacggggta
     7141 aaccggtagc cgaatgccga gcggacgcac gagcaacggc gaataactgc cgcaggcgag
     7201 cacgcaggca tccgcggcca atgcgccctt gtcggtgtgg acgcgggtga tgcggtcgcc
     7261 gtcggcttcg agccgctcga tgcgagtatt gagcgcgaag ctcacgcctt gcgattcggc
     7321 aagtttcgcg agcgattgcg tgaattgaaa acaatcgcca gattcgtcgc cgggaaaatg
     7381 caggccgccg gcgatttttt cctgcgagac acgcaacgcg ggttcctgtg ccatgcagcc
     7441 ggcgcgatcg agcagccggc aatccacgcc ccattgtttg agcagggcgg catcccgcac
     7501 cgcgtcatcg agttcgtgtg ccgtgcgaaa caattcgagc acgccagtgg tgtgctcgtc
     7561 gtaacggatg ccggtttcgc gccgcagcgc cttcaggcat tcgtggctgt aacgcgaaag
     7621 gcgcagcatg cgttcccggt tgacggcgta acgttcggcg gtgcagttgg cgagcatgct
     7681 gaagagccat ttccacatga ccggatcgag acgcgggcgc agcaccagcg gcgaatgcgg
     7741 gcgcagcatc catttcagcg ccttgagcgg aatgccgggc gtggcccagg gcgtgccggc
     7801 gccccacgac acctgaccgc cgttggcctg gctcgtttcc aggcccggtg ctgcttgccg
     7861 atccacgaca gtgacctcgt ggcccgcgcg ccgcagatac caggcgctgg tgacgccgat
     7921 caccccgctg ccaaggacaa gaactttcat gggataccta aatgaggaaa cggattttgt
     7981 catttatgcc gtgtcgtgga gtctgccact ggtttgcgcg cctgcggtag tggttgttcc
     8041 cgtgcatgga tatgctgtcg cgacgatgat gtattgattt ttaaaggttt ttagggcgat
     8101 tcggcccggc aaatacccat ttttaactgg catgtgaatt gcttacttaa agttggttca
     8161 gggtatttgc cgccgccttt gcggcatagc cggttttctt acaaagaggc aaggagttct
     8221 ccatgtcgat gatcaagcat gttattcgat ttatccagtg ggcggcggta tcgctgctgc
     8281 tcggcgtcgc ggtgacgcag ggcgccatcg aaaagtccac ggcggaattt ccctatcgtt
     8341 cgcaatatga cgtcaacatc atcgagacgg cggaattcag cgtcaagctt agcgcggcgg
     8401 tggcggtcga tgtccgaacg aagtatgaat atgaaacgct gcatataaag ggcgcgatca
     8461 acatcccgct cgacaagaat ttcatcgaca acgtgcgcaa gctacgcgcc aaggatacgc
     8521 gcccgatcat tttttactgc aacggcaaga cctgccgaaa gagttacgac gcggcgacca
     8581 tggccggcaa agcgggaatc agcgatgtct acgcctacga cgcaggcata ctggtctggg
     8641 ccgagacgca ccccgaaaac accgttttgc gcggcaaggc gtcgattcag cgcagcgatc
     8701 tgatcggcga aaaggatttc gccagttacg tgctcgcgcc caaggcattc gcgacgttgt
     8761 tcgagcagct tggcgacaag gccatggtgc tcgatatacg cgaccgcgta caacgcgaca
     8821 atccggtatt cgggcttgtg gaggaacgca tcccgctcga cgacgcggcg cgcctggacg
     8881 cgtttatcga gacagtgaaa aaacatcgta agacgctgct ggtgtacgac aaggccggtc
     8941 atcagatccg ttggtttcaa tattacctgc gcgaaaaggg tgtacgcgat tatcgcttcc
     9001 tcaagggcgg ttccgaaggc tacttcgtgg aaacgctggg tctcaaggag ttcggcaaga
     9061 aatagcccgg ctgtttatta agacagcaac ggggagtcgt ctcgatatat accggcgcgc
     9121 gttcgcgcgc cgttccccgt ttttcaggaa agcgagccat ttttttgcaa agacgccggg
     9181 acggagggcc ggggattctt caatatgcgt tctacgttag ccaggcggtg aagctcgcca
     9241 ccggctcgcg cgcggtacgg tagttgttca ccggcgcccc ggcatcggga tagccgagcg
     9301 ccatgccaca gaggagggcg cgggtgtcgg gcacattcac cagtttgcgc acaatgtcgg
     9361 ggtattccgc gatcgatgcc tgcgggcagg tggcgaggcc atgcccgagc gcggagagca
     9421 tgacgttctc gatgaacata cccatgtcca cccaggaacc tttttcgagc gcacggtcga
     9481 gaaaaaacag cagtcccacc ggcgcgccga agaaggaata attgttgttc caggccttga
     9541 ggcgcgcgtc cttgtcgtca cg
//
LOCUS       DBNGZE010000183         2781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1416079, whole genome shotgun sequence.
ACCESSION   DBNGZE010000183 DBNGZE010000000
VERSION     DBNGZE010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1416079"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1469
                     /gene="gatB"
                     /locus_tag="AC16FV_04830"
     CDS             36..1469
                     /gene="gatB"
                     /locus_tag="AC16FV_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR2849425.1"
                     /translation="MEWETVIGLEVHAQLMTKSKAFSGAATKYGAEPNTQACAVDIAL
                     PGVLPVFNKEAARMAVKFGLAIGAAINHRSVFARKNYFYPDLPKGYQISQYELPVVAK
                     GQITIDVDGVEKIVGITRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPDMR
                     SPKEAVAYLKKLHELVRYLEICDGNMQEGSFRCDANVSVRPKGEKKFGTRAELKNINS
                     FRFVEKAIEFEVERQIELIEGGGKVIQETRLYDSDKDVTRSMRSKEEAMDYRYFPDPD
                     LPPLVLDQAFIDAVKTTLPELPNTKRARFMKDYGLNAYDAGVLTSSRELADYYEQVVR
                     ESKADSKIAANWVTGELAAFLNRDNKDITASPVTVAMMSGLLKRLADNTISGKIAKEV
                     FEAMWNGEGDADAVIEKKSLKQITDTGAIEKVIDEIIAKNPKQHEQYRAGKEALFGYF
                     VGQVMKATQGKANPAQVNELLKKKLSA"
     gene            1673..2059
                     /locus_tag="AC16FV_04835"
     CDS             1673..2059
                     /locus_tag="AC16FV_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2849426.1"
                     /translation="MTVHLDHLMVPSRNKVASAKLLAELLGVPWSETGVGPFVPVYVN
                     DGLTIDFDEWTDPIPMIHYCFRVSQYDFNAILARIKAAGIPYRSSVHGPVDFQIDTQH
                     GGSIVYWNEPDGHQWELLTVSYARQA"
     gene            complement(2151..2219)
                     /locus_tag="AC16FV_04840"
                     /pseudo
     CDS             complement(2151..2219)
                     /locus_tag="AC16FV_04840"
                     /inference="COORDINATES: protein motif:HMM:NF046915.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2262..>2781)
                     /locus_tag="AC16FV_04845"
     CDS             complement(2262..>2781)
                     /locus_tag="AC16FV_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HIR2849427.1"
                     /translation="VIAFCIVVATAVTLNQQGITDIQTSAQAAEALRPIAGKFAFELF
                     AFGIIGTGLLAVPVLAGSAAYAVSELFGWKAGLSHGFHEARGFYLIIAVATGVGTLMS
                     VFEVDPIKALVWSAIVNGVISVPIMTVMMVIGQSKNLMGRYTISTRHRVFGWAATGIM
                     AVAVAFMFATSF"
BASE COUNT          643 a          797 c          788 g          553 t
ORIGIN      
        1 gactggcacc agcgcatgcc gaagggattt agctgatgga atgggaaacg gtcatcggtc
       61 ttgaagtcca cgcccagctc atgacgaaga gcaaggcgtt ctcgggcgcg gccacgaaat
      121 acggcgcgga gccgaatacg caggcctgcg cggtggatat tgccctgccg ggcgtgttgc
      181 cggtattcaa taaagaagcc gcgcgcatgg cggtgaagtt cgggcttgcc atcggcgcgg
      241 ccatcaacca ccgctcggtg ttcgcgcgca agaattattt ctaccccgac ctgcccaagg
      301 gctaccagat cagccagtac gaactgccgg tcgtggccaa gggccagatc accattgacg
      361 tcgatggcgt ggaaaagatc gttggcatta cgcgtgcgca tctcgaagaa gacgctggaa
      421 aaagcctgca cgaggacttc cacggcatga ccgggatcga cctcaaccgc gccggcacgc
      481 cgttgctcga aatcgtctcc gagccggaca tgcgctcgcc caaggaggcc gtggcctacc
      541 tgaaaaaact gcacgagctg gtgcgctatc tcgaaatctg cgacggcaac atgcaggaag
      601 gttcgttccg ttgcgacgcc aacgtctcgg tgcggcccaa gggcgagaag aaattcggca
      661 cgcgcgccga gctcaaaaac atcaattcct tccgttttgt cgaaaaggcc attgaattcg
      721 aggtcgaacg ccagatcgag ctgattgaag gtggggggaa agtgattcag gaaacgaggc
      781 tgtacgactc ggacaaggac gtcacgcgtt ccatgcgcag caaggaagag gcgatggatt
      841 atcgctactt ccccgacccc gacctgccgc cgctggtgct ggatcaggcc ttcatcgatg
      901 ccgtcaaaac caccttgcct gaattgccga acaccaagcg agcgcggttt atgaaggatt
      961 acggtctgaa tgcctacgat gccggcgtat tgacctcctc gcgcgagctg gcggattact
     1021 acgaacaggt ggtgcgcgaa tcgaaggccg attccaagat tgcggccaac tgggtcacgg
     1081 gggaactggc ggccttcctc aaccgcgaca acaaggacat taccgcttca ccggtgacgg
     1141 tggcgatgat gtcaggcctg ctcaagcgtc tcgctgacaa caccatctcc ggcaagatcg
     1201 ccaaggaagt cttcgaggcc atgtggaacg gcgaaggcga cgccgatgcc gtgattgaaa
     1261 agaagagcct gaaacagatc accgataccg gcgcgatcga gaaggtcatt gacgagatca
     1321 ttgccaaaaa ccccaagcag catgagcagt accgtgctgg caaggaagcg ttgttcggct
     1381 atttcgtcgg tcaggtgatg aaggccaccc agggcaaggc caatcccgcc caggtcaacg
     1441 agctgctcaa gaagaaactc agcgcctagg tgcccacacc ccgtgggcac gtgtaacttt
     1501 ggcctgtttg tattcgcctc gccaatcttg acctgtgctc acgagctgtc gggatgggtg
     1561 acaggcacca ctccatgcag tattttgttg ctttataaag caacatctag gttgcctaat
     1621 tctggttgag cattacttgt aacctgacac ccttcaagaa caggagggca cgatgaccgt
     1681 tcatcttgat caccttatgg tgccgtcccg caacaaagtg gcatcggcga agctattagc
     1741 cgaactgctt ggggttcctt ggtcggaaac gggtgtaggg ccgttcgtgc ccgtgtatgt
     1801 gaacgatgga ttaactatcg attttgatga atggaccgac cccattccca tgattcacta
     1861 ctgttttcga gtgagtcagt acgacttcaa tgccatcctt gcgcgaatca aggcagctgg
     1921 aataccgtac cgtagcagcg ttcatgggcc ggtcgacttc cagattgaca cgcagcacgg
     1981 cggcagcatc gtttactgga acgaaccaga tggtcatcaa tgggagctgt tgacggtcag
     2041 ctatgcacgt caagcgtagc aattttatcc tgagacggtg tgcccaaccc attggtttaa
     2101 gcagattacc ttcggcagcc gctgaactcg aacattagac gtacgcgaca tcaaatctct
     2161 atctgcgttc ccagctcgat cacccggttc gcgggcaggt tgaaatattc caccgggctg
     2221 ctggcgttgc gcgttccggc ccggtttgat gccccgcatg ctcaaaaact cgtcgcgaac
     2281 atgaacgcca ccgccaccgc catgattcct gtggcggccc agccgaatac acggtgacgc
     2341 gtgctgatgg tatagcgtcc catgaggttc tttgactgcc cgatcaccat catcactgtc
     2401 atgatcggta ccgagatgac gccattgaca atcgcgctcc aaaccagcgc tttgatgggg
     2461 tcaacttcaa agacgctcat cagggtaccc acgcccgtgg ctaccgcgat gatgaggtaa
     2521 aatccccggg cttcgtgaaa tccgtgcgac agccccgcct tccagccgaa taattcactg
     2581 accgcgtagg cggcggaccc cgccagaacc ggaaccgcca gcaacccggt tccgatgatt
     2641 ccaaacgcga acagctcgaa tgcaaatttg ccggctattg gcctcaatgc ctccgccgcc
     2701 tgggccgaag tctgaatatc cgtgatgcct tgctgattca gggtgacggc ggtcgcgaca
     2761 acgatgcaga acgcgatgac g
//
LOCUS       DBNGZE010000184         2132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1207481, whole genome shotgun sequence.
ACCESSION   DBNGZE010000184 DBNGZE010000000
VERSION     DBNGZE010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1207481"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /gene="treZ"
                     /locus_tag="AC16FV_04850"
     CDS             complement(<1..1997)
                     /gene="treZ"
                     /locus_tag="AC16FV_04850"
                     /EC_number="3.2.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033942 -
                     4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HIR2849428.1"
                     /translation="MDQAARQLTAGLGNGTISSSSTGGIAPPKPIPPAHPAKPLPTAS
                     AQTARENSQAVTRRLPVGAEVFTDRGVHFRVWVPRGARVEVVLEGQRSHADAQSSAID
                     LTREEDGYFSGWAPEAAASTRYRFRLDNKSELYPDPASRFQPEGPHGPSVVVDPASFE
                     WHDGSWRGITLEGQVIYEMHVGAFTPDGTWLSASRELEELKTLGISVIEVMPIHEFAG
                     RFGWGYDGVNLFAPTHLYGEPDDLRRFVDTAHSLGLGVILDVVYNHFGPDGNYLKDFS
                     PDYFTDRYHTDWGEAINFEGAKAAPVREFIISNAGYWIDEFHFDGLRLDATQNIYDRS
                     AEHILAAVTRKVRHAAAGRGTIVVAENEPQHTNLARPADQGGYGIDGLWNDDFHHAAM
                     VALTGHNEAYYTDYFGQPQELISAIKWGHLYQGQRYKWQKRRRGTSTFGLKPATFIHY
                     LQNHDQIANSGTGRRIHYLSHPGCYRAMTALLLLAPQTPMLFQGQEFAASSPFNYFAD
                     HQPELAAAVRQGRREFMSQFPSVASSEAQEGLPDPADRATFEACILDFSERIKHAPVY
                     RLHRDLLKLRKEDAVFSAQRPGGVDGAVLNGQALVLRFFGSGNDDRLLLVNLGVDLHL
                     NPAPEPLLAPPEGEIWTLLWSSEDPSYDGAGSKASDDEENW"
BASE COUNT          469 a          641 c          590 g          432 t
ORIGIN      
        1 atccagttct cctcgtcatc ggacgctttc gagccagcgc cgtcatagga aggatcctcg
       61 ctcgaccaga gtagcgtcca gatttcccct tcagggggcg ccagcaacgg ctcgggtgcc
      121 gggttcaaat gcaagtcgac cccaaggttc acgagcaaaa gccgatcgtc gttaccgctg
      181 ccaaaaaacc gcaacaccaa tgcctggcca ttcaacaccg caccgtcgac gccacccggt
      241 cgttgcgcgc tgaacaccgc gtcctccttg cgcaacttaa gcagatcacg atgcaagcgg
      301 tacaccggcg catgcttaat acgctcggaa aagtccaata tgcaagcttc gaaagtggcg
      361 cgatcggcag gatccggcag cccttcctgg gcctcggaag acgcaacgct ggggaactgg
      421 ctcataaatt ctcggcggcc ctggcgcaca gccgcggcaa gctccggctg atgatcggca
      481 aaataattga atgggctcga cgcggcgaac tcctgcccct gaaacagcat cggcgtctgt
      541 ggcgccaaaa gtaacagcgc ggtcatcgct ctataacaac ccggatgact gagataatgg
      601 atacgccgac ccgtgccgct attggcgatc tggtcatgat tttgaagata atgaataaaa
      661 gttgccggtt ttagcccgaa agtggaagtg ccgcgccttc gcttctgcca tttatagcgc
      721 tgcccctggt ataggtggcc ccacttgatc gctgagatca attcctgggg ttggccaaaa
      781 tagtcggtat aataggcttc gttgtgcccg gttaacgcta ccatcgccgc atgatggaaa
      841 tcatcgttcc acagtccatc gatgccataa ccgccttgat cagccggccg cgccagattg
      901 gtgtgctgcg gctcgttttc agcgacgacg atggtcccgc ggcccgcggc ggcgtggcgg
      961 actttcctgg tgacggccgc cagaatgtgt tcagccgaac gatcgtatat gttttgcgtg
     1021 gcatccaaac gcagaccgtc aaaatggaac tcatcaatcc agtagccggc attgctgata
     1081 ataaattccc gcaccggcgc cgctttcgca ccctcaaaat taatcgcttc gccccagtcg
     1141 gtgtgatagc gatcggtaaa atagtcaggg gaaaaatcct tgagatagtt tccatcggga
     1201 ccaaaatggt tgtaaaccac gtccaggatg acgcccaatc ccagactatg cgccgtatcg
     1261 acaaagcgac gcaagtcgtc gggttcgccg tagagatgcg tgggcgcgaa cagattcacg
     1321 ccatcgtaac cccagccgaa gcggccggcg aattcgtgaa tcggcatcac ctcgattacg
     1381 ctgatgccca acgtttttag ctcctctagt tcgcggctgg cggagagcca ggtcccgtcg
     1441 ggcgtgaagg cgccgacgtg catctcgtag atgacctgcc cttcgagcgt tatcccccgc
     1501 catgaaccgt cgtgccattc aaaactggcc ggatcgacga ccaccgaagg gccgtgaggc
     1561 ccctctggtt ggaaacgaga agcaggatcg ggataaagct cgctcttatt gtcaagccga
     1621 aatcgatacc gagtactagc ggccgcttcc ggggcccaac ccgaaaagta accatcctct
     1681 tctcgcgtca aatcaatggc ggatgattgc gcgtcggcgt gactccgctg tccttccaag
     1741 accacttcaa cgcgcgcgcc acgcggcacc cagacccgga aatgaactcc ccggtcagta
     1801 aacacctcgg ctcctacggg cagccgcctg gtcaccgctt gagaattttc cctggcggtt
     1861 tgagcggaag ccgtcggcag tggtttcgct gggtgggccg gtggaattgg ttttggcggg
     1921 gcgatcccgc ccgtagacga gctggagatt gttccatttc ccaggccggc ggtgagttgt
     1981 cgagccgctt gatccatgac tattcgccca tggttatcgt cgcttgcaaa gcaaaattcc
     2041 aatcgcttga taaagcaatg tgggtagagc caaatccacg gcgccgagac ccggggtcat
     2101 cacgcggcac ggcgcggttc tacctacacc ct
//
LOCUS       DBNGZE010000185         5565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1064027, whole genome shotgun sequence.
ACCESSION   DBNGZE010000185 DBNGZE010000000
VERSION     DBNGZE010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1064027"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="AC16FV_04855"
     CDS             complement(<1..1655)
                     /locus_tag="AC16FV_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849429.1"
                     /translation="MKPQLKTARGNRLLSAAIGILLTSTVGFASEAWADTVSTSDALL
                     SSSSSGPTLPKNTFNILMNYELGMHCTGFEFSYCCVLPPYNSILAQVVKTQQHLPTVH
                     SHDLGSTDIHGTVFPTLLEGDPRVGLDFLGRPTVLRDTELDSNGNFKKYVLRYWHDAQ
                     PRNDGRGAPQTSTLISQVEGNTLLMWNTQFDAAATDSGNKLIYGDYGSTVPAVLPPDP
                     NAHQASSPKNNVLLGNGSYADPEDNYANAWLNHLYIYADLEGSNPTANISAEKDKIRL
                     GLRNQNASTPFATVLPQNSGPAFHPLGPGSTPGLQNVLTFSGDTGTVVFTQMKVLENL
                     PVMLTSPRIWEALGLPLDPFEDTINFFGDPGKVTEETIRPYVQMKAALHNYDPTQLGG
                     IGTAVLQNGNPVIGMGDAPIDIPNCERCHALPASVNNPNNANISATDIYRPYTAAKVQ
                     LERDFWNAYYNIDVQAGDSDWYSRLKAAAISILSIHDNEHRTDFTLNYPQTGGNPPQL
                     TRLGHESVLCQRCHADNVIAVVKSATSRTPEQLIPPLTEALHN"
     gene            complement(1624..1809)
                     /locus_tag="AC16FV_04860"
     CDS             complement(1624..1809)
                     /locus_tag="AC16FV_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849430.1"
                     /translation="MDVIRLTDKFVNSFLDALISRLFLTRIIGTQIAPGFEKYVPRHG
                     HEQKGGVNETATEDRSR"
     gene            1971..3356
                     /locus_tag="AC16FV_04865"
     CDS             1971..3356
                     /locus_tag="AC16FV_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR2849431.1"
                     /translation="MKRNTVIESLINAQESPAVVIDKHYRIIAANRAYCTSYGVEADT
                     VIGHCCHEISHHSPVPCHQNGEDCPHKTVLTTSKPFKVTHTHYDYANQPDRMHIKAYP
                     IETANGDRLVLETIHRLTPPVHLTCEEMQMVGHAPVFLQCLQTMRLAAKSYIPVLLYG
                     ETGVGKEIAAQYIHEQSGRRAKSYVELNCAAIPEALFESELFGHERGAYTGCVGLKQG
                     LLETADKGTLFLDEIGELPLAMQVKLLHVFDSGEFRRLGGTSTIKVDVRIITATNQNL
                     MAMVTDGSFREDLYFRIAGIKIDIPPLRERRSDIPALVEALIKRMCHREKTERCYLTQ
                     AAMDCLMSYHFPGNVRELLNILQQARALTPDGIITPEHIRLGENHHSPRPSGDTLPTH
                     PVPAGRTAAATETNAPRSLADAEARHIATLLEHHDHNRHAVAETLGISERTLYRKIKR
                     YKLNSHAAVEE"
     gene            complement(3615..3703)
                     /locus_tag="AC16FV_04870"
     tRNA            complement(3615..3703)
                     /locus_tag="AC16FV_04870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3667..3669),aa:Leu,seq:taa)
     gene            complement(3752..3825)
                     /locus_tag="AC16FV_04875"
     tRNA            complement(3752..3825)
                     /locus_tag="AC16FV_04875"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3791..3793),aa:Cys,seq:gca)
     gene            complement(3876..3951)
                     /locus_tag="AC16FV_04880"
     tRNA            complement(3876..3951)
                     /locus_tag="AC16FV_04880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3916..3918),aa:Gly,seq:gcc)
     gene            complement(4091..4312)
                     /locus_tag="AC16FV_04885"
     CDS             complement(4091..4312)
                     /locus_tag="AC16FV_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR2849432.1"
                     /translation="MAKLRVLSGREVCRILGKHGFVEVRRRGSHVVMQRKIETGSITV
                     PVPDHSEIKIGTLLSIIRQSGLPRSEFES"
     gene            complement(4305..4529)
                     /locus_tag="AC16FV_04890"
     CDS             complement(4305..4529)
                     /locus_tag="AC16FV_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR2849433.1"
                     /translation="MAKNLYTAILEREGDLYVALCPELDIASQGKTVEDATANLKEAV
                     ELFLETADPSEIKQRLHDQILVTTFEAAHG"
     gene            complement(4748..5335)
                     /gene="pgsA"
                     /locus_tag="AC16FV_04895"
                     /pseudo
     CDS             complement(4748..5335)
                     /gene="pgsA"
                     /locus_tag="AC16FV_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345722.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
     gene            5370..>5565
                     /locus_tag="AC16FV_04900"
     CDS             5370..>5565
                     /locus_tag="AC16FV_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849434.1"
                     /translation="MRADRVGAWYAPYITGLSSPRKRGSSAVYVIARKARDTIQNLDA
                     RLRGHDGCYNFPLFIPHHGTH"
BASE COUNT         1249 a         1535 c         1590 g         1191 t
ORIGIN      
        1 ttgttgtgaa gggcctcggt cagcggcggg atcagttgtt ccggcgtgcg gctggtcgcc
       61 gacttgacca cggcgatcac attgtccgca tggcacctct gacacagcac gctttcgtgc
      121 ccgagccggg tgagctgcgg aggattcccg cccgtctgcg gataattcaa ggtgaaatcc
      181 gtgcggtgct cattgtcgtg gatcgacagg atgctgatcg cggccgcctt cagacgcgag
      241 taccagtcgg agtcaccggc ctggacgtcg atgttgtaat acgcattcca gaaatcgcgc
      301 tcgagctgca ccttcgccgc cgtgtaggga cggtagatat cagttgcgct gatgttcgca
      361 ttgttcgggt tgttgacgct agcggggagc gcgtggcagc gctcgcagtt gggaatatcg
      421 atcggggcat cgcccatgcc aatgaccggg ttgccattct gcagcactgc agttccgatc
      481 ccgcccagtt gggtcgggtc gtagttatgc agcgccgctt tcatctggac ataaggccgg
      541 atagtctctt cggtgacctt tcccgggtcg ccgaagaaat tgatcgtatc ctcgaatgga
      601 tcgagcggca ggcccaaggc ttcccagatc cgcggcgagg tcagcatgac aggcagattt
      661 tccagcacct tcatctgggt gaagaccacg gtgcccgtgt cgccggagaa ggtcaggaca
      721 ttctgcagac cgggtgtcga tccgggtccc aaggggtgaa aggccggacc gctgttctgc
      781 ggcagaaccg tcgcaaaggg agtcgacgca ttctgattgc gcaaacccag gcggatcttg
      841 tctttctcgg cgctgatgtt tgcggtggga ttacttcctt ccaggtcggc atagatgtac
      901 agatggttca accacgcgtt ggcgtagttg tcctcgggat cggcgtaact gccgttaccc
      961 agcaagacat tattcttggg agatgaagcc tgatgcgcat taggatccgg aggaagaacc
     1021 gcaggaaccg ttgacccgta gtcgccgtag atcagtttgt tgccgctgtc ggtagcggcg
     1081 gcgtcgaatt gggtgttcca catcagcagg gtgttaccct ccacctggct gatgagggta
     1141 ctcgtctgcg gagcgccgcg accgtcgttc cgcggctggg cgtcatgcca gtaccttaat
     1201 acgtacttct tgaaattccc gttcgaatcc agctcggtgt cccgcagcac cgtgggccgg
     1261 ccgagaaagt cgagccccac cctgggatcg ccctccagca gcgtggggaa caccgtccca
     1321 tgtatgtccg tgctgcccag gtcgtgcgag tgtaccgtcg gcaggtgttg ctgggttttg
     1381 accacttgag ccaggatgga attatacggc ggcagcacgc aacaatagga gaactcgaat
     1441 ccggtgcagt gcatgcccag ctcatagttc atcaatatat tgaaagtgtt cttgggtagc
     1501 gtcggaccgc tagaagaact gctgagtaat gcatcgctgg tagaaaccgt gtcggcccat
     1561 gcctccgagg caaatccgac ggtcgatgta agcaagatac cgattgccgc cgacaacagc
     1621 cgattaccgc gagcggtctt cagttgcggt ttcattgact cctccttttt gttcatgccc
     1681 atgacgggga acatattttt cgaaaccggg agcaatctgc gtgccaataa ttcttgtgag
     1741 aaagagccgt gaaatcagcg catctaaaaa tgaattgacg aatttgtcag tcaggcgtat
     1801 gacatccatg tcattcacat gacagaaatg tcaatttgaa tcttatactg acaatatatc
     1861 gggataccgg gggtttcacg ggtttattgc cattaacaaa aatactcaac agtacagtat
     1921 gcaatcaagc cgtggcatga acaaacaacg ggtaattgaa ggaggagatc atgaaacgga
     1981 acaccgtgat tgagtcgctg atcaatgccc aggaatcgcc cgccgtggtc atcgacaaac
     2041 attatcgaat catcgccgcc aatcgggcct actgtacatc ttatggcgtg gaagctgaca
     2101 cggttatcgg acactgctgt cacgaaatat cccaccattc tcccgttccc tgtcaccaga
     2161 atggagaaga ttgtccgcat aagacggtgc tgaccactag caaaccgttc aaggtcacgc
     2221 acacccacta tgattacgcc aaccaacccg accgcatgca catcaaggcc tatcccatcg
     2281 aaaccgcgaa tggcgaccgg ttagtccttg aaaccatcca tcgcctgacg ccccccgtac
     2341 acctgacctg tgaagagatg cagatggtcg ggcacgcccc cgtgttcctg caatgcttgc
     2401 agaccatgag actggctgcc aaatcctata taccggtgct gctttacgga gaaaccggcg
     2461 tgggcaaaga aatagccgca cagtatatcc atgagcaatc aggacgccgc gccaaatcct
     2521 acgtcgagct gaattgcgcg gcgatcccgg aggcgctttt cgagagcgag ttgtttggcc
     2581 acgagcgcgg cgcctacacc ggatgcgtgg ggctcaagca agggctgctc gagacggcgg
     2641 acaaagggac cttgtttctg gacgagatcg gcgaactgcc attagccatg caggtaaagc
     2701 tgctgcacgt attcgattcg ggcgaatttc gccgcctggg cggcacatca acgatcaagg
     2761 ttgacgtgcg catcatcact gccaccaacc agaacctgat ggccatggtc actgatggca
     2821 gtttccggga agacctttat ttccgcattg ccggtatcaa gatagacatt ccaccattac
     2881 gcgagcggcg gtcggatata cccgcattgg tagaggcact gatcaagcgc atgtgccacc
     2941 gggaaaagac ggaacgctgc taccttaccc aagcagccat ggactgcctc atgagttatc
     3001 actttcccgg caacgtgcgc gagctgttga acatcctgca gcaagcccgc gcgttaactc
     3061 cggatggaat tattacgcct gaacacatcc gccttggcga gaatcatcat tcaccgcgtc
     3121 cttccggcga taccttgccg acccatccag tcccggctgg acgaacagca gccgcgacag
     3181 aaacaaatgc gccccgatcg ctggcggacg ccgaagcccg ccatatcgcc acgctgctgg
     3241 aacatcacga tcacaaccgt cacgccgttg cggaaacgct gggtatcagc gagcgcacgc
     3301 tttatcgcaa gatcaaacgt tacaagctta attcgcacgc ggcggtggag gaataacgta
     3361 atgtggtgcc cggggccgga ctcgaaccgg cacggtgttg ccaccaaggg attttcttac
     3421 ccactacggc tttcgccgcc gcgccggagc gcctttgggg tctggacttt acctttgccg
     3481 tggcgccggg agacacggta ggcaggagcc gtcaaaactg taggttgggg tgaatgaaat
     3541 gaaccccaac cccttgggcc aagctgttgg ggttcgctac gctcacccca acctacgaaa
     3601 atcagtagtg ttggtggtgc ccagggccgg aatcgaaccg gcacggaggt tgccctccaa
     3661 cggattttaa gtccgttgcg tctaccaatt ccgccacccg ggcttcgggg tgagtgtaat
     3721 ggatggggtg ggactgcgaa aggaaggaaa ttggaggctg gggccggaat tgaaccggcg
     3781 tacgcggctt tgcaggccgc tgcataacca ctctgctacc cagcctaaac ctgaacaaaa
     3841 aagggaggac ccgtctgggc gcctccctgt gtgtctggag cgggaaacga ggctcgaact
     3901 cgcgacctta accttggcaa ggttacgctc taccaactga gctattcccg caggagaagt
     3961 gcattgtaag gacggcggac attctgtcaa gaaatttgct gagatttcct tgttttatag
     4021 ctgtttgcgt tcaccgtaga catgcagaga aagatttatc agggtaaagc aaggcgaatc
     4081 ttggcttacc tcaggactca aattcgctgc gcggtagccc actttgccga atgatggaaa
     4141 ggagcgtacc gatttttatc tctgaatgat ctggaaccgg aacggtgatg gagcctgtct
     4201 cgatcttacg ctgcatgacg acatggctgc cgcggcggcg gacctcaaca aacccatgct
     4261 tacccaagat acggcagact tcacgtcccg agaggacgcg cagtttagcc atgcgccgct
     4321 tcgaaggtgg ttaccaatat ctggtcgtgt agacgttgtt taatttcgga aggatcagcg
     4381 gtttccagga ataactccac cgcctctttc agattggctg tggcgtcttc cacggttttt
     4441 ccctgactgg ctatatccag ttccgggcac aaggcgacat ataaatcgcc ctcacgttct
     4501 aaaattgccg tatatagatt tttagccatt tggtactcct gtaccggaag ataatagcac
     4561 gccctgccct tcagccttga aataaagtcc tgggagcggt atcggactcg ctcttggaca
     4621 ttctggaccg ccccacctga cttcccgtcg agggtcagga aatcttgatg tcgcaaaatg
     4681 cgacctcaag atatgatgaa tttattgaaa gaaattgtaa atcgcaatga atttattgtg
     4741 aaaagtttta cggcgcgcgc ggggcgatga ggttgggcca ggcggccttg aggtagatga
     4801 gcatggacca cagcgtcagc gccgcggcga ggtagagcag gatctcgccg atacgaaaga
     4861 tcggcaggtc ccagaacgac ctctgccaca acaggaacgg gatggcgagc atctgggaag
     4921 cggtcttgat cttgcccacg tacgacaccg ccacgcgcga gcgcttgccc acctccgaca
     4981 tccattcgcg cagggccgag acggtgattt cgcgtcagac gatgaccagc accaccacgg
     5041 caaacaggcg gatgttcagg acgtgcctct gtaccatcgg gtctgccacc agcatgacca
     5101 gcgccgccgc gaccatgagc ttgtcggcca ccgggtcgag aaaggcgccg aagggcgagg
     5161 tctgctccca cttgcgcgcc agatagccgt cgagccagtc ggtgagtgcg gccaggataa
     5221 acagcccgct ggcgatcatg tgggcccagg gatgggtcca gtagtacgcg acgatgaacg
     5281 ccgggatcag cgcgatgcgc aggagcgtga ggatgttggg ggtgtttaga agcatatgat
     5341 tttcttgaac cgtttatgta gggcgcgcca tgcgcgccga tcgagttggc gcgtggtacg
     5401 cgccctacat tactggattg tcatccccgc gaaagcgggg atccagtgct gtttatgtca
     5461 tcgcgcgcaa agcgcgcgat accattcaaa acctggatgc ccgcctacgc ggacatgacg
     5521 gctgttataa ctttcctctt tttattcctc accatggaac gcatc
//
LOCUS       DBNGZE010000186         2001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_304915, whole genome shotgun sequence.
ACCESSION   DBNGZE010000186 DBNGZE010000000
VERSION     DBNGZE010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_304915"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16FV_04905"
     CDS             complement(<1..220)
                     /locus_tag="AC16FV_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2849435.1"
                     /translation="MPAETRLFLCRSDNYGVLLHDPESGATATIDAPEAAPIEAALKA
                     TGWKLTDILVTHHHADHTDGIKTLKEKYK"
     gene            343..1074
                     /locus_tag="AC16FV_04910"
     CDS             343..1074
                     /locus_tag="AC16FV_04910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2849436.1"
                     /translation="MSIDVVDLRNFYGQRLGVVARRFIGRGIRTLWGDTAGQRVLGIG
                     YATPYLGLFREEAERCLALMPAGQGVVKWPTARPALSALVDENELPLADSAVDRVMLV
                     HALEMTNDPGALLREVWRVLASGGRLLAVVPNRRGLWARMDTTPFGHGRPYSRSQINQ
                     LLRETWFTPTGWSEALYVPPVPRGWFLRSAVAWERTGATLSAPFAGVHLVEATKQVYR
                     AIPARREKRRLVPALEPALAPSPGT"
     gene            complement(1163..1945)
                     /locus_tag="AC16FV_04915"
     CDS             complement(1163..1945)
                     /locus_tag="AC16FV_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HIR2849437.1"
                     /translation="MRNGQKRMRGRGNHRKSQNPLTRVYESNGPDVKIRGTASHIAEK
                     YIQLARDAQGSGDPVAAENYYQHAEHYFRLIAAAQEQFRQQNPYYNQQPQQQGAPGQQ
                     TADEGFDGDDDDAQPFMGQNEPSYAPQPQVQQAQPYLPRDGQPFPPRDQQVHNRPQHQ
                     PQPYQQPNQGGHEGGQEVGGLPAFITGSGGQPQQNPAQSQNGHDQGDRGQDRFGRHRR
                     RRHRGGGGFRPDRPDFGGGHAQGAGPQPAQQDESGGSDPRPD"
BASE COUNT          304 a          591 c          649 g          457 t
ORIGIN      
        1 acttgtattt ttctttcagt gtcttgatcc cgtcggtgtg gtcggcatga tgatgagtga
       61 ccaggatgtc agtcaatttc cagccggtcg ctttcagcgc tgcctcgatc ggcgcggctt
      121 cgggagcgtc gattgtagcg gtagctccac tttctgggtc atgcagcagc accccgtaat
      181 tgtcggatcg gcagaggaaa agacgggttt ccgcgggcat gggcttggtc ctgttcacgt
      241 actggaatgg gttaaccgta tcacgcgggt gaatgatcgt gcacgtccgg gtatcccgcg
      301 ccgttagcgg tttgctacgc tgtttcatgg tagtttcgcc gcatgtcgat cgacgttgtg
      361 gacctgcgca atttctacgg ccaacggctg ggggtagtcg cacgccgctt tattggtcgc
      421 ggcatccgca cactgtgggg tgatactgct ggccagcgtg tgctcggcat cggctatgcc
      481 acgccttatc tcggcctgtt tcgcgaggag gccgagcgct gcctcgccct catgccggcc
      541 gggcaggggg tcgtgaaatg gccaaccgcg cgcccggcat tgtccgcgct agtagacgaa
      601 aacgagctac cgcttgctga ctccgcagtc gaccgcgtca tgctggtgca tgcgctggaa
      661 atgacgaacg atccgggtgc cctgttgcgt gaagtttggc gagtgctggc atctggagga
      721 cggttgctgg cggtggttcc aaaccggcgc ggtttgtggg cgcgcatgga caccacaccg
      781 ttcggacatg gtcggcctta ttcgcgcagc caaatcaacc agctcttgcg cgagacctgg
      841 ttcacgccga ctgggtggag cgaagcactc tatgtaccgc ccgtgccacg tggttggttc
      901 ctgcggtcgg cggtggcatg ggagcgcacc ggagcgacgt tgtcggcacc attcgccggc
      961 gtacatctgg tggaagcgac caagcaagtt tatcgcgcaa tcccggcgcg acgcgagaag
     1021 cggcgattgg tgcccgcgct ggagccggca ttagcgccgt cgccggggac gtgatccgcc
     1081 gctgatccaa actccgattt tggctggtcc cgattctgca gcgcaccact tcgtgcagcg
     1141 ctgcgcccgg gacaagggcc gctcagtccg gccgcgggtc gctgccgccg ctttcatctt
     1201 gttgggccgg ttgtggcccc gcgccttgcg catggccgcc accaaaatcg ggacggtccg
     1261 ggcggaagcc accgccgccg cgatgacgac gacgacgatg cctgccgaaa cgatcctgac
     1321 cacgatcgcc ctgatcgtgg ccgttctggc tctgggcggg attctgttgc ggctggccgc
     1381 cgctgccagt gatgaacgcc ggtaggccgc caacctcttg tccgccttca tggccgccct
     1441 ggttcggttg ctggtagggc tgcggttgat gctgcggacg gttgtgcacc tgctgatcgc
     1501 gcggcgggaa tggctggcca tcccgtggca aataaggctg cgcttgctgc acttgcggct
     1561 gcggcgcata gctcggttcg ttctggccca tgaaaggttg agcatcgtca tcgtcgccat
     1621 caaagccctc gtctgcagtt tgctgaccag gcgctccctg ttgctgcggt tgctggttgt
     1681 aatagggatt ctgttggcgg aactgttcct gcgcggcggc gattaggcga aaataatgct
     1741 cggcgtgctg atagtagttt tcagcggcca ccggatcacc cgagccctgt gcgtcacgcg
     1801 caagctggat atatttttcc gcaatatgcg aagcagtgcc gcggattttg acatcgggac
     1861 cgtttgattc ataaacgcgc gtcagcggat tttggctttt gcggtggttg ccgcgtcccc
     1921 gcatgcgctt ctgaccattt ctcatgatgt gcctgttttc gcctttcgtc tgtctcggcg
     1981 ttcgccgccg aacgctcaac c
//
LOCUS       DBNGZE010000187         4281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_443890, whole genome shotgun sequence.
ACCESSION   DBNGZE010000187 DBNGZE010000000
VERSION     DBNGZE010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_443890"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="AC16FV_04920"
     CDS             <1..2116
                     /locus_tag="AC16FV_04920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2849438.1"
                     /translation="HKLCESTGWLLGQAWIPGETGRLECMPVWHGEAKGLERFRKASL
                     DMTFSPGIGLPGRVWESKKPVWIKDVTQEGNFPRADAATEAGLRAGLGIPVTVGEEVI
                     AVLEFFMREPREEDERLTGVVSSAAAQLGSVIQRKRAETALRRSENQLRAIVDAEPEC
                     VKIVKADGTLVQMNAAGLAMIEASRLEQVIGKPIFSLLVPEYREPFRAFVANVLRGNK
                     GIMEFEIVGFKGTHRWMETHSVSLPGEEGGPPLMLSITRDITERKQAERRLRQLAHFD
                     SLTDLPNRVQFIERLEQALADADQNERLVGVVFLDLDRFKYINDSLGHEKGDSLLREV
                     AIRLNGVLRRGDTVARLSGDEFALVLADMGHVDDAIHVAQKILDVFHQPFRVAGHDLF
                     VTASMGITLYPFDDRGAHELLRNADVAMYRAKESGKNNYQFYVAEMTAKVAERLTLEN
                     DLRSALERGEFSVNYQPIADCQSGKIVGMEALLRWKHPERGMISPALFIPLAEETGYI
                     ISIGEWVLRTACEQCRRWQKMGFPSLYVAVNLSSRQFHQKDLPASIYRILQETGLNPA
                     SLGLELTEGLVMQQAEASVNTLRELKAMDIRISIDDFGTGYSSLSYLKRFPIDVLKVD
                     QSFVRDIPKDEDDAAIASTIVTMAHSLGLKVVAEGVETVDQLKFMREHRCDAMQGYYL
                     SKPLPPEQFESFLKNGSRLDVA"
     gene            2354..2869
                     /locus_tag="AC16FV_04925"
     CDS             2354..2869
                     /locus_tag="AC16FV_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR2849439.1"
                     /translation="MKKFLLLLPLIVFLNDAVLAAEKPAIAYVDVRKVLLESKVGKKN
                     KAEFEKMIKEKESVLNKEEEKLKAMQEAFQKDQLLMTDEQKKAKQKAFQEKADAYQKM
                     VRDAKQEINKKDNEYAGKSLTEIRGIVAELAKEMKLSLVLEASESGLLYAEEGMDLTS
                     KVMEKYDAKAK"
     gene            2960..>4281
                     /gene="leuS"
                     /locus_tag="AC16FV_04930"
     CDS             2960..>4281
                     /gene="leuS"
                     /locus_tag="AC16FV_04930"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR2849440.1"
                     /translation="MDEQYLPDKVEKDAQACWEQHRMFKAVEDPKREKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVIARYQRMQGRNVLQPMGWDAFGLPAENAAMANGVPPAKWTY
                     DNIAYMKKQLKSLGFAIDWERELATCRPEYYRWNQWLFLRMLEKGIAYKKTGVVNWDP
                     VDQTVLANEQVIDGRGWRTGAVVEKREIPMYYLAITQYAQELLADLDQLPEWPERVRA
                     MQANWIGKSVGVRFAFPYKLDGREEKLRVFTTRADTIMGVTFCAVAAEHPLATHAAKN
                     NPKLAAFIEECKRGSVMEADLATIEKKGMPTGISVTHPLTGEQIPVWVGNYVLMGYGE
                     GAVMAVPAHDERDFHFARQYKLPIKQVIAVGNEQFSLDGWREWYADKEHGRCVNSGKY
                     DGLGYSAAVDAIAADLKAKGLGDKQVMWRLRDWGISRQRYWGCPIPII"
BASE COUNT          942 a         1215 c         1337 g          787 t
ORIGIN      
        1 gcacaagctg tgcgaatcca ccggctggct gctgggacag gcctggatac cgggtgaaac
       61 cggtcgtctc gagtgcatgc cggtgtggca tggagaggcg aaaggcctgg aacgctttcg
      121 caaggcgagc ttggacatga cattttcccc gggcatcggg ttgcctggcc gtgtgtggga
      181 atcgaagaaa ccggtatgga tcaaggacgt gacccaagag ggtaattttc cacgcgccga
      241 cgccgccacg gaagccggcc tgagagcggg gttgggaatt ccggtgacgg tgggggaaga
      301 agtcatcgcc gtgctcgaat ttttcatgcg cgaaccgcgc gaggaagacg aacgcctgac
      361 cggtgtcgtt tccagcgccg cggcccagct gggttcggtg atacagcgca agcgcgcgga
      421 gacggcgctg cgccgcagcg agaatcagct gcgcgcgatc gtcgacgccg agccggaatg
      481 cgtcaagatc gtcaaggcgg acgggacgct ggtgcagatg aacgccgcgg gcctggccat
      541 gatcgaggcc agccgactcg agcaggtgat cggcaagccg atattcagtc tgttggtgcc
      601 ggagtatcgt gaacccttcc gcgcattcgt cgcgaacgtc ctgcgtggca acaagggcat
      661 aatggaattc gagatcgtcg gattcaaggg gacgcaccgg tggatggaga cgcactccgt
      721 ttccctgccc ggcgaagaag gcggtcctcc gctcatgctg agcatcacgc gcgacatcac
      781 cgagcgcaag caggccgagc ggcgtctcag acaactcgcg catttcgaca gcctgaccga
      841 tcttcccaac cgcgtgcaat tcatcgagcg gctggaacag gccctggccg acgcggacca
      901 gaatgaacgg ctggtgggcg tcgtctttct cgatctcgac cggttcaagt acatcaatga
      961 cagtctcggc catgaaaagg gggacagtct gctgagggag gtcgcgatac gcctgaacgg
     1021 cgtgttgcgc cggggtgaca cggtcgcgcg cctgagcggc gatgaattcg cgctggtgct
     1081 ggcggacatg gggcacgtgg atgacgccat tcacgtggcc cagaaaatcc tggacgtctt
     1141 ccatcagcca tttcgtgtcg ccgggcatga tttgttcgtc acggccagca tgggcatcac
     1201 gctttatccc ttcgacgacc ggggcgccca cgagctgttg cgcaacgccg acgtggccat
     1261 gtaccgggcc aaggagtccg gcaagaacaa ctaccagttc tacgtggcgg aaatgaccgc
     1321 caaggtggcc gagcggctga cactcgaaaa cgatctgcgc tcagcgcttg agcgcggaga
     1381 attttccgtg aattaccagc cgatcgccga ttgccagtcc gggaagatcg ttggcatgga
     1441 ggcgttgcta cgctggaagc atccggaacg cggcatgatt tcaccggcgc tgttcattcc
     1501 gctcgccgag gaaaccggct atatcatttc catcggcgaa tgggtgttgc gcacggcctg
     1561 cgagcaatgc cggcgctggc agaaaatggg attcccgtca ctctatgtgg cggtgaatct
     1621 ttcctcgcgc cagtttcatc agaaggattt gcccgcatcc atctaccgca ttttgcagga
     1681 aaccgggctt aatccggcct cgcttggcct ggagctgacg gagggtctgg tcatgcagca
     1741 ggccgaggcc tccgtgaaca cattgcgcga gctgaaagcg atggatatca ggatatccat
     1801 cgacgatttt ggcaccggtt attccagcct cagctacctc aagcgtttcc cgattgacgt
     1861 gctcaaggtc gaccagtcgt tcgtgcgcga tattccgaag gatgaagatg acgccgccat
     1921 tgccagcacc attgtcacca tggcccacag cctggggctg aaggttgtgg ccgagggcgt
     1981 cgaaaccgtc gatcagctga aattcatgcg cgagcatcgt tgcgacgcca tgcagggtta
     2041 ctatctcagc aaacccctcc cgccggagca attcgagagt tttctgaaaa acggaagccg
     2101 tctcgacgtt gcctgatggc gcgtcaccgg tgcttccggc gctgttgttt tccattccgc
     2161 aacttcctta tccggtcgtc accgcacgca cccgcgatca cggcgcgaaa gcggtgcaaa
     2221 tgcagttccg cccgctgagc tcgctcgtct taccgatgat aacgtagccc tgttcgctac
     2281 gctcactcgt cttgctgata taattcgccc gtcccgccgt tggcggtttt tgtcatcttc
     2341 caaggactga aatatgaaaa aatttcttct gctgctgccg ttgatcgtgt ttctgaatga
     2401 cgccgttctc gccgccgaga aaccggccat cgcctacgtg gatgtgcgca aggtgctgct
     2461 ggagagcaag gtcggcaaga aaaacaaggc cgagttcgag aaaatgatca aggaaaagga
     2521 aagtgtcctg aacaaggagg aggagaagct caaggccatg caggaggcat tccagaagga
     2581 ccagctgctg atgacggacg agcagaaaaa ggccaagcag aaggctttcc aggaaaaggc
     2641 cgacgcctac cagaagatgg tcagggacgc caaacaggag atcaacaaga aagacaacga
     2701 atacgccggc aaatcgctga cggaaatacg cggcatcgtc gcggaactgg ccaaggaaat
     2761 gaagctgagc ctcgtgctgg aggcatccga atccggcctg ctgtacgccg aggagggcat
     2821 ggacctgacg tcgaaggtga tggagaagta cgacgcgaag gccaaataat cttcgctcat
     2881 gcgccgccct gctgaaaaag acggaggccg tgaattacgg tatccttccg gccctcagct
     2941 ttgagccgaa ccaaacccga tggacgagca atacctgcct gacaaggtag aaaaagacgc
     3001 ccaagcctgc tgggagcaac accggatgtt caaggcggtg gaagacccga aacgggagaa
     3061 attctactgc ctgtcgatgt ttccctatcc ctccggcaag ctgcacatgg gccatgtgcg
     3121 caactacacg atcggcgatg tcatcgcgcg ctaccagcgc atgcagggcc gcaacgtgtt
     3181 gcaacccatg ggctgggacg ccttcggcct gccggcggag aacgcggcga tggcgaacgg
     3241 cgtgccgccg gcgaagtgga cctacgacaa tatcgcttac atgaagaagc agttgaagtc
     3301 gctcggtttc gcgatcgact gggagcgtga gcttgccacc tgccggccgg aatattacag
     3361 gtggaaccaa tggctgttcc tgcgcatgct ggaaaagggc atcgcctaca agaaaaccgg
     3421 cgtggtgaac tgggacccgg tggaccagac ggtgctcgcg aacgaacagg tgatcgacgg
     3481 gcgcggctgg cgcaccggcg ccgtggtcga gaagcgcgag atcccgatgt attacctggc
     3541 gatcacgcaa tacgcccaag aactgctggc cgatctcgat caattacccg aatggcccga
     3601 gcgcgtgcgc gccatgcagg ccaactggat cggcaagagc gtgggcgtgc gtttcgcctt
     3661 cccatacaag ctggatggac gcgaggaaaa actccgggtc ttcaccacgc gcgccgacac
     3721 catcatgggt gtcactttct gcgccgtggc ggcggagcat ccgctcgcga cgcacgccgc
     3781 gaagaacaat ccaaaactcg ctgcattcat cgaagaatgc aagcgcggct cggtcatgga
     3841 ggccgacctc gccaccattg aaaagaaagg catgccgacc ggcatcagcg tgacacaccc
     3901 actcaccggc gagcagattc cggtgtgggt cggtaactac gtgctcatgg gttacggcga
     3961 aggcgcggtg atggcggtac cggcgcacga cgagcgtgat ttccattttg ccaggcagta
     4021 caagcttccc atcaagcagg tgattgcggt cggaaacgag caattctctc ttgatggttg
     4081 gcgggaatgg tacgcggaca aggagcatgg ccgttgcgta aattcgggca agtacgacgg
     4141 tcttggctac agcgccgcgg tcgatgccat tgccgccgac ctgaaagcga aaggtctcgg
     4201 tgacaagcag gtcatgtggc gcctgcgcga ctggggcatt tcgcgccagc gctattgggg
     4261 ctgcccgata cctattattc a
//
LOCUS       DBNGZE010000188         1976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_651612, whole genome shotgun sequence.
ACCESSION   DBNGZE010000188 DBNGZE010000000
VERSION     DBNGZE010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_651612"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..432)
                     /locus_tag="AC16FV_04935"
     CDS             complement(172..432)
                     /locus_tag="AC16FV_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003592126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR2849441.1"
                     /translation="MDDTQVGWIAAIIIGGIAGWLAEQFMKSDMGLLMNIVLGIVGAA
                     VASAILSFFGVHLGGWVGYLIAGFIGGVLLIWIVRQVRGRTV"
     gene            1341..1934
                     /locus_tag="AC16FV_04940"
     CDS             1341..1934
                     /locus_tag="AC16FV_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2849442.1"
                     /translation="MLASQMTRRLFLSRTFEGAVDTILDDVIALLGAEYGNVQLSIGD
                     ELVIAAQRGLSADFLRTFRRVKKDDGSACGRALRLGETVIIRDVENDPEFAAFRPDAK
                     KAGFRSVQSTPLFTKDGNVLGMVSTHFAHAHEPTPIEMQALKAYSIVAAEHLNKLLGD
                     VPLATKARQMSEELYTRTLGQQGVSAEPRSPGISTSP"
BASE COUNT          432 a          578 c          548 g          418 t
ORIGIN      
        1 cgcgtggtgg ccttcaatac cgccgaaggc tggtcacgcg acgtgaccga agaaatagcg
       61 gcgaggctgc tagacctcaa tgcgcaaggg cgcatgctgg gagcatcagc acgcgatttt
      121 gttgagcgcc gggaaagctg taacggtcgt tgtcctagtt actcggcctc atcaaacggt
      181 ccggccccgt acttgccgga cgatccagat cagcaagacc ccgccgataa acccggcaat
      241 caggtagcct acccatcccc ctaggtggac cccaaagaag ctcaaaatgg cgctggcaac
      301 cgccgccccg acgataccga gcacgatatt catcaggagt cccatgtcac tcttcatgaa
      361 ttgttctgct agccagcctg caattccgcc gatgataatg gctgcgatcc agcctacttg
      421 tgtgtcatcc atggctcgtc tccccaaaaa ggcgcattcc ctgcgccgat ttcgattttc
      481 taatgcgcaa agtccattaa caagcctgta gacactcctg cgcgatcctc tttactcaca
      541 tggcgaatat cgacattgcg attctgactc ttctgccgtc gatgcccgca tgggcgctgc
      601 cgtgggtgcg ggtcgtggca aaggcacccc taccgccgcg agtgcgcgaa aaccgtatcc
      661 cgcaccgaca ttccagcaac gttcactggc gtaccaataa agatgcgcct tgggaaattt
      721 ggcgcgggcc tcgcttgccg tcatgcaagt cggcgagcca taagcccatg acggcccgat
      781 catgatggcg atggcaagga taaggataat gcgtattgtc attgcccccg atacgatacc
      841 agccccttca tgcggggaaa aagatcgccg gtttgcctgt gaatatcctg ttaataaccg
      901 ggccgtgggg ccaaccgcgc ccacgatcga gaaacccagc aatccggtga cttcataaaa
      961 cgaaccaggg ctgggccgct attcacgtcg ctgcgggggc ggaccctcct ggccgccttc
     1021 taaccaagct tggttgtttg cgagattctc ccacgccttc tccatgcgct tgaagcgagc
     1081 cctgtcctgc gaagtagtgg cggtctgcgc tttctcagcg caattctcgg cgttcttacg
     1141 aaggtcatcc gacttttgca tcgtgcgcct cctaaccgtg gaaacttgca attaacctat
     1201 ccgctaggaa ccggttccag ttcacggcca gcgtaaggac aaagctggtt tctttcgcgg
     1261 actgcggaac tgcgggcagt cgccatcgtt atggttttgg gcttaaccaa ccattgtaga
     1321 gaggacgggg gtaacgtcgc atgctggcgt cgcaaatgac ccgacgttta ttcctgagta
     1381 ggacgttcga gggagccgtt gataccattc tcgatgacgt catcgcgctg ctaggcgcag
     1441 agtacggtaa cgtccagcta tcgattgggg atgagttggt cattgccgcg cagcgcgggc
     1501 tttcagcaga ctttttgaga acttttaggc gggtcaaaaa agacgacgga tctgcgtgcg
     1561 gccgggctct gcggctcggg gaaacggtca tcattcgcga tgtagaaaac gatcctgaat
     1621 ttgccgcctt ccggccggac gccaagaaag ccgggtttcg ctcggtccag agtacgccgc
     1681 tcttcaccaa agacggaaac gtgctgggga tggtgtccac gcattttgca catgctcatg
     1741 agccgacacc aattgagatg caggcgttga aggcatacag catcgtagct gctgagcacc
     1801 tgaataaact gctgggtgac gtaccgctcg ccaccaaggc aagacaaatg agcgaggagt
     1861 tgtacactcg caccttagga caacaagggg tatcagctga gccccgatct ccgggcattt
     1921 ccacgtcgcc ttaaacgcgc ctcttccacg cgcatggcgc ggcagccatt ggagag
//
LOCUS       DBNGZE010000189         1693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_374848, whole genome shotgun sequence.
ACCESSION   DBNGZE010000189 DBNGZE010000000
VERSION     DBNGZE010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_374848"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC16FV_04945"
     CDS             <1..392
                     /locus_tag="AC16FV_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR2849443.1"
                     /translation="AAEGLRIVVNPNADGGMGTSLACGVAAVEADGWVIALADMPAIQ
                     STTIQAVAEALTVGAPLVAPVYHGQRGHPIGFARRFQAALTALTGDRGGRDILARHSN
                     ELRLIEVDDPGVLVDIDHAADIVVEEH"
     gene            complement(560..>1693)
                     /locus_tag="AC16FV_04950"
     CDS             complement(560..>1693)
                     /locus_tag="AC16FV_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2849444.1"
                     /translation="LFDKKLSQEEAWIRQGVKARRTRNEGRVRALEALREQRRQRRDR
                     VDKVDLRMEEGAASGQLVFEAEHVDFNYGDTAVIKDFSVRIMRGDRIGIVGPNGAGKS
                     TLIKLLLGELPLTRGHLRRGTRLQVAYFDQQRELLDSNAMVMDSVGEGSQTITINGRS
                     QHVAGYLQSFLFPPERLRSPVSTLSGGERNRLMLARLFARPANLLVMDEPTNDLDVET
                     LELLEELLLEYQGTLLLVSHDRAFLDNAVTSTLVFEGGGRIGEYVGGYTDWLRQKKSV
                     PKSESPAKTGKPATTVEAPRAKSRKLSYKDQRELEALPGKIEALEAEQSRLQASVSHA
                     GFYQQPGEAIAATLARLQVVTAELEECYVRWEILEAQQTTTGG"
BASE COUNT          291 a          517 c          526 g          359 t
ORIGIN      
        1 tcgcggccga gggtctgcgt atcgtcgtca atccgaatgc cgacggcggc atgggcacga
       61 gccttgcctg cggcgttgcc gctgtcgagg ccgatggctg ggttatcgcg ctggccgaca
      121 tgcccgcgat tcagtccacg actatccagg ccgtggccga agcactcacc gttggcgcgc
      181 ctctcgtcgc acccgtctat cacggtcagc gtggccatcc catcggtttc gcacgccgtt
      241 ttcaggcggc gctgactgcg ctcactggtg acaggggtgg acgtgacatc ctggcgagac
      301 actccaatga gttgcgcctg atcgaggtgg acgatccggg tgtgctggtc gatattgatc
      361 acgccgcgga tatcgttgtc gaggagcact aaggaacctc tgattaagtc tcccgttcgc
      421 cctgagcttg tcgaagggtg aacgggaaat accgcaaata acggataatt cgtccgttca
      481 tggttcgaca ggctcaccac gaacggactt gttcagaggt tccctaacac tctgctccaa
      541 agtaatcgac aggaaagttc tatccgcccg tggtggtctg ctgcgcctcc agaatctccc
      601 agcggacata gcattcttca agctcagcgg tgaccacttg caggcgcgca agcgtggcgg
      661 cgatggcctc gcccggctgt tggtaaaaac cggcatggct gacggatgcc tgcaaacgcg
      721 actgttccgc ttccagcgct tcgatctttc ccgggagggc ttcgagctcg cgttggtcct
      781 tgtacgacag cttgcgagac ttggcgcgcg gggcttccac ggtggtcgcg ggtttgccgg
      841 tcttggcagg tgactcggat ttcgggacgg attttttctg ccgcagccag tcggtataac
      901 cgccgacgta ttcgccgatg cggccgccgc cttcgaacac cagcgtgctg gtgacggcgt
      961 tgtcgaggaa cgcacggtcg tggctcacca gcagcaacgt gccctggtat tcgagcagca
     1021 gctcttccag cagttcgagc gtttccacgt ccagatcgtt ggtgggttcg tccatcacca
     1081 gcagattggc cgggcgggcg aacaggcgcg ccagcatcag ccgattgcgc tcgccgccgg
     1141 agagcgtgct taccggggag cgcagtcgct ccggggggaa cagaaagctc tgcaaataac
     1201 cggcaacgtg ttggctgcgg ccgttgatcg tgattgtctg gctgccttcg ccgacgctgt
     1261 ccatcaccat ggcgttggag tcgagcagtt cacgctgttg atcaaaataa gcgacttgca
     1321 accgcgtgcc tcggcgcaag tgtccgcgcg tcagtggcaa ttcgcccagc agaagtttga
     1381 tcagcgtgga tttgccggcg ccgttgggac caacgatgcc gatgcgatct ccgcgcatga
     1441 tgcgcaccga gaaatccttg atcacggcgg tgtcgccgta attgaaatca acgtgctcgg
     1501 cctcgaacac cagttggccg gacgctgcgc cctcctccat cctcagatcc accttgtcca
     1561 cacggtcgcg gcgctggcgg cgctgttcgc gcaaggcttc gagggcgcgc acgcggcctt
     1621 cgttgcgcgt gcgccgggcc ttcacgcctt gacgtatcca cgcttcttcc tgtgagagtt
     1681 tcttgtcgaa cag
//
LOCUS       DBNGZE010000190         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_305335, whole genome shotgun sequence.
ACCESSION   DBNGZE010000190 DBNGZE010000000
VERSION     DBNGZE010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_305335"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="AC16FV_04955"
     CDS             <1..567
                     /locus_tag="AC16FV_04955"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0061711 - tRNA
                     N(6)-L-threonylcarbamoyladenine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR2849445.1"
                     /translation="DDAVGEAFDKTAKLLGLGYPGGPAIARAAQGGDPKKFKFPRPMT
                     ERRKTQGAHPGLDFSFSGLKTAVVTAVQNQTLTPQLIADIAASFEEAAVDVLAIKCEW
                     ALERTGAKQLVVAGGVSANLKLRERLKQLSEKLGVRVYFPRPEFSTDNGAMIAYVGYL
                     RLSAGQSEPLAFNARARWRIEGIGAVKT"
     gene            complement(564..1151)
                     /gene="plsY"
                     /locus_tag="AC16FV_04960"
     CDS             complement(564..1151)
                     /gene="plsY"
                     /locus_tag="AC16FV_04960"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR2849446.1"
                     /translation="MMWLYFLPLLAYLLGSVSSAVVIARMMGLQDPRDVGSGNPGATN
                     ILRYGGKAAAILTLAGDILKGVIPVLVARALTTDAVIITLTGFAAFLGHLFPVFFGFR
                     GGKGVATALGVWLALNPWVGLLLLATWVLMAILFRYSSLSALTASVAAPLYVAWLSPD
                     MPYLITMVVMSAILIFRHRANIRNLLAGAETKIGR"
     gene            1189..1542
                     /gene="folB"
                     /locus_tag="AC16FV_04965"
     CDS             1189..1542
                     /gene="folB"
                     /locus_tag="AC16FV_04965"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR2849447.1"
                     /translation="MDIIYLHDLKIECIIGIWEWERRIKQTIIFDLDMAADIRRAAAT
                     DKIEDTLNYKAVSKRLGEFVGNSQFQLVETLAEKVAEILLTEFNLQWVRVRVNKKGAV
                     RGATDVGVIIERGKA"
     gene            1548..2042
                     /gene="folK"
                     /locus_tag="AC16FV_04970"
     CDS             1548..2042
                     /gene="folK"
                     /locus_tag="AC16FV_04970"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR2849448.1"
                     /translation="MPRVYVGIGSNIDRENSIRGAVRELTVHFGSLTLSPVYESKALG
                     FEGENFFNLVAGFDSVEPIEQIEETLSRIELRLGRVRQENRFSARTLDLDLLLYGDAV
                     RHDSKVNLPHPDIRRYAFVLRPLADIAPDLRHPETGRTCAEMWQQFDAGKQETWKTDF
                     NPRT"
     gene            complement(2054..>2542)
                     /locus_tag="AC16FV_04975"
     CDS             complement(2054..>2542)
                     /locus_tag="AC16FV_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2849449.1"
                     /translation="LDVLINNASRFYPTPVGEATESQWDDIVGTNLRAPYFIAQAAVP
                     HLKKSGGCIINIADIYGDRPLIGYPIYSVAKAGVIMLTKALARELGPEIRVNAIAPGV
                     ILWPESDLDEMSKQRIISRTPLKRSGDTSDVAQTVLFLVRDAGFITGQVLAVDGGRWV
                     VM"
BASE COUNT          548 a          780 c          784 g          430 t
ORIGIN      
        1 gatgacgcag tgggcgaggc cttcgacaaa accgccaagc tgctcggact cggttacccg
       61 ggcggcccgg ccatcgccag ggcggcgcaa ggcggcgatc ccaaaaaatt caaattcccg
      121 cgccccatga cggaacggcg caagacccag ggcgcgcatc cgggactcga tttcagtttc
      181 agcggcctca agaccgccgt ggtcacggcg gtgcagaacc agacgctcac gccgcaactc
      241 attgccgaca tcgcggcctc gttcgaggaa gccgcagtgg atgtgctcgc catcaaatgt
      301 gagtgggcgc tggaacggac cggcgcaaag caactggttg tcgcgggcgg cgtcagcgcc
      361 aatctgaaat tgcgcgagcg cctgaaacag ctctcggaaa aacttggcgt tcgtgtgtac
      421 ttcccgcgtc cggaattttc caccgacaac ggcgccatga ttgcttatgt cggttacctg
      481 cgtctctcgg cgggacagag cgaaccgctg gcgttcaacg cgcgggcgcg ctggcgtatc
      541 gaggggatcg gtgcggttaa aacctaacga ccaatcttgg tctcggcacc ggcgagcaga
      601 ttgcggatgt tggcgcggtg ccggaagatc aggatcgcgc tcatgacgac catggttata
      661 aggtaaggca tgtccggcga cagccacgcc acgtacagcg gcgcggccac cgaggccgtc
      721 agggccgaca gcgaggaata gcggaacaga atcgccatga gcacccaggt ggcgagcaac
      781 aacaaaccga cccacggatt gagcgcaagc cacacgccca gtgccgtcgc cacgcccttg
      841 ccgccgcgga agccgaaaaa caccggaaac aggtgaccga gaaacgcggc gaaccctgta
      901 agcgtgatga tcacggcatc cgtggtcaac gcgcgcgcga ccagcaccgg aatcacgccc
      961 ttcaggatgt ctcccgccaa ggtcaggatg gccgccgcct tgccaccata gcgcaggatg
     1021 ttggtggcgc cgggattccc ggagccgaca tcgcgcgggt cctgcagtcc catcatgcgg
     1081 gcgatgacca cggcgctcga caccgagccg agcaggtagg ccagcagtgg aaggaaataa
     1141 agccacatca tttaaagtga gcggtgtttt tcgttatagt ggcgacccat ggatattatc
     1201 tacctccacg acctcaagat cgagtgcatc atcggcatct gggagtggga acgccgcatc
     1261 aagcagacca tcattttcga tctcgacatg gccgcggaca tccgccgcgc cgcggccacc
     1321 gacaagatcg aggacaccct caattacaaa gccgtttcca aacgcctcgg cgagtttgtc
     1381 ggcaactcgc aattccagct ggtggaaacc ctggcagaaa aggtcgcgga aatcctgctg
     1441 accgaattca atctccagtg ggtgcgcgtg cgcgtcaaca agaagggcgc cgtgcgcggc
     1501 gcgactgacg tgggcgtgat catcgagcgc gggaaggcct gaactccgtg cctcgggtgt
     1561 acgtcggcat cggcagcaac atcgacaggg aaaacagcat ccgcggcgcg gtgcgcgaac
     1621 tgacggtaca tttcggttcc ctcacgctct ctcccgtcta tgaaagcaag gccctcggtt
     1681 tcgagggcga gaattttttc aatctcgtgg ctggcttcga cagcgtcgaa cccatcgaac
     1741 agatcgagga aacgctctcc cgaatcgagt tgcggctcgg acgcgtgcgt caggaaaatc
     1801 gcttcagcgc gcgcacgctc gatctcgacc tgctgctcta cggcgatgcc gtgcggcacg
     1861 acagcaaggt gaacctgccg catcctgaca tccggcgtta tgccttcgtg ctgcgcccgc
     1921 tggcggacat tgcgccggat ttgcggcatc cagaaacagg ccggacttgc gccgagatgt
     1981 ggcagcagtt cgacgctgga aaacaggaaa cgtggaagac ggattttaat ccgcggacct
     2041 gaagcgggcg gaattacatc accacccagc gcccgccgtc caccgccagc acctgcccgg
     2101 tgatgaaacc ggcgtcgcgg acgagaaaca gcacggtttg cgccacgtcg ctggtgtcgc
     2161 cagaacgctt gagcggggtg cgcgaaatga tgcgctgctt ggacatctcg tcgaggtcac
     2221 tctcgggcca caggatcacg ccgggggcga tggcattgac ccgtatctcc ggacccagct
     2281 cgcgcgccag cgccttggta agcatgatca cacctgcctt ggcgacgcta tagatcgggt
     2341 aaccaatcaa tggacggtcg ccgtagatat cggcgatgtt gataatgcag ccaccagact
     2401 tcttgagatg cggcaccgcc gcctgtgcga tgaaatatgg cgcccgcagg ttggtgccca
     2461 cgatatcgtc ccactgggat tcggtggcct cgccgacagg tgttgggtaa aaccgcgagg
     2521 cgttgttgat caacacatcc ag
//
LOCUS       DBNGZE010000191         3501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_930868, whole genome shotgun sequence.
ACCESSION   DBNGZE010000191 DBNGZE010000000
VERSION     DBNGZE010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_930868"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="AC16FV_04980"
     CDS             <1..393
                     /locus_tag="AC16FV_04980"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR2849450.1"
                     /translation="EHKLEHHFEIDSAGTHAYHVGEPPDERAQAACARRGIDISRLSG
                     RKAVVGDIQKFDYILAMDRENYENLLEICPSGFESRIRLFMEFAPNRPEKEVPDPYFG
                     GAGGFDRVLDMIEDAAQGLLEDIRRTRL"
     gene            complement(1122..2816)
                     /locus_tag="AC16FV_04985"
                     /pseudo
     CDS             complement(1122..2816)
                     /locus_tag="AC16FV_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003119808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
BASE COUNT          573 a         1057 c         1110 g          761 t
ORIGIN      
        1 gaacacaagc tggaacatca cttcgaaatc gattcggcgg gcacccatgc ctatcacgtc
       61 ggcgagccgc cggacgagcg cgcgcaggcc gcctgcgcgc gtcgcggtat tgacatcagc
      121 cgcctgagcg ggcgcaaagc cgtggtgggt gacatccaga agttcgatta catcctcgcc
      181 atggatcgcg aaaactacga aaacctgctt gagatctgtc cgtcgggttt tgaatcccgc
      241 atccggctgt tcatggagtt cgcaccgaac cggccggaga aggaagtgcc cgatccgtat
      301 tttggcgggg ccggcggctt cgaccgcgtg ctcgacatga tcgaggacgc cgcgcaaggc
      361 ttgctggaag acatccggcg cacccgtttg tagccctcga aaaaaaaccc gccttgcggc
      421 gggttttttg tttacttgtt gcctcggtca tcctcgttgt taaaccgggt ttcgacctga
      481 accagctccg gcttgggagc gggctcactg ctgtccgaac cgtgatgcgt ttcatgctcg
      541 gacggactcg tcgaaggggt gtgccagtcg tcattccgct ccgacggctg ctcccaggat
      601 gaaaccggag gcggggttgg cgtgaaggat tccgtcacgc cgccgaccgt gctggatggc
      661 gcctgctgtg tctcctcgcg cgcgggacgc ggattttgtt cctgccgctc cggcgctcca
      721 ttcgattcca tggcgccgtt gcgcgcgttg aaatcctggc gctcctgcgg cggcctgcct
      781 tcctggcccc ggccaccgcg acgacggccg cgacgacggc gtcttccacc gccaccttca
      841 tgccgttcac gctgcgcctg gttggcgtcg ccgccgccgg ggccaccggc agcctgttgt
      901 ggctgcggtg gacgcggacg gttttgatcc tggccctgac cttgttggcc accgggacgg
      961 cggtcgggat gcccgtgacg ctcgtgccgt tcatggcgct gaccaccgtg atgcgggcga
     1021 tgatgcggac gcccgccgcc gtgaccgcga cccggctgtt gtgggcgcat tggacggctc
     1081 tccggtgctg atggtttctt ttcttcccct tctttcttgc cgaacaggcc ggtgatgatt
     1141 ttcttgatga aaccaccgcc attggcggca gccggcttcg gacgttccgg cggcgcggcg
     1201 gcatgcgtga gcgggccgat gaccggcttt tccgcttccg ccgacaccgg ggtgggctgg
     1261 gcgggcttgc gctcttcctc ttcctcgtcc tcgaccaggt cgatctcgta gctcggcacg
     1321 tcagcctcgg cctcgtattc gtcgatgcgc aggcggcgga tgtggtagtg cggcgtttcc
     1381 atgtccgaca tcggactgat catgatcggc gtgccgatgc gcgattcgat ggcgctcatt
     1441 tccatgcgct tctcgttcag caggaaggtc gcggtgtcga tcggcaggtg cacgtgcacg
     1501 gccgcggtgt tttccttcat ggcctcttcc tggatcatgc gcaggatcga gagggcggag
     1561 gactgcacgc tgcggatgtg accggtgccg cggcagcgcg ggcagatcag gctggaggac
     1621 tcaccgagcg agggacgcag acgctggcgc gacatctcga gcagaccgaa acgcgatatg
     1681 cggccgacct gggtgcgggc gcggtccacc ttgagcgatt cgaacatgcg attttccacc
     1741 tcgcgccggt tacgctcggg cgtcatgtcg atgaagtcga tgacgatcag gccgccgagg
     1801 tcgcgcaggc gcaactggcg cgcgatctct tccgcggctt cgaggttggt gttgagcgcg
     1861 gtctcctcga tgtcggcgcc cttggtggcg cgcgccgagt tgacgtcgat cgtgacgagg
     1921 gcttcggttt tatcgaatac aattgcgccg cccgattcca ggcgcacctc gcgcgagaag
     1981 gcggattcga tctggtgctc gatctggaag cgcgagaaca gcggcgtttc gtccttgtac
     2041 agcttgagct tgtccaggaa ttgcggcgcc acctgctgca tgaatttcga catgcgctcg
     2101 tggatttccg gattgtccac catgacctcg ccgatgtcgg ccttgagata atcgcgcgtg
     2161 gcgcgcacca cgaggttgct ttcctggtag atcaggaacg gcgcggcgcg ctcctgcgag
     2221 gccttgtaga tggcgtccca caggtggatc aggtagtcga ggtcccactg cagctcttca
     2281 gccgaacgac cgatgccggc ggtgcgggcg atgacggaat aatcgtcggg cagcttgagc
     2341 tgtgacatgg cctcgcgcag ttcggcgcgt tcctcgccct cgatgcgacg ggaaatgccg
     2401 ccgcccttgg ggttgttggg catgagcacc agataacggc cggcgaggct gacgaaggtc
     2461 gtgagcgccg cacccttggt gccgcgttct tccttgtcca cctgcaccag cagttcctgg
     2521 ccttcgcgga tcacgtcctt gatgcgcacc tgactcatcg gggtgcgttc gttgtaaccc
     2581 tgaaagtaaa tacgggaaat ttccttgagg ggcaggaagc cctggcgctc ggagccgtag
     2641 tccacgaagg cggcttccag gctcggttcc acgcgggtga ccttgccctt gtagatgttt
     2701 ccttttttct gctggtaggt cgaggattca atgtcgaggt cgagcagttt ctggccgtcg
     2761 acaacggcca atcgcaactc ctcgggatga gttgcgttaa agagaatacg cttcattgtt
     2821 gatatatgtc cccgacgccc ggtttggcgc gctgctccgc ccgcgctgtt gattcagcgc
     2881 gacatggcga aacgtcatcc tgccgacagg gtgcagagac cgctaatgta gggttgacgg
     2941 ccttctgctt gaaatccgcg cttattgctg tgcgcggttg tgcaattcag actttaatca
     3001 cgccgtgaac agttttgctg tcccacgcgt cgtctgcctg tatgtgtcgg caggtgctcg
     3061 ctttatgagc gtctaatcca tacgcctcta caggaagtag gggagaggcg tgttacccat
     3121 ttgggctctg tccgctatgt gttcgcaaga gacaccggat cgtgtgggcc ggtgggcaac
     3181 ggtgcggcaa agcccgcgcc ggcttttacc tggcgcgaaa atccgttgca tttcgtgtac
     3241 ttgtgtcaaa ttctgaagaa tttactaaaa gtacaccttt gcactatagc aaacgaattg
     3301 actctccagc aatctgttat tatcaccccg gcatgaaaaa ggccgtagac cccgcaccct
     3361 ccgccgtccg ttacctcgaa attgacgaaa ccacgtcgaa cgcgacgtgg gtgcggccgc
     3421 tttgtacgcc atatccgaca tcgagccgaa aaacgccgag ccactcgaac cccacgccgg
     3481 tcgtgacccg cgtaccggga g
//
LOCUS       DBNGZE010000192         2303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1695184, whole genome shotgun sequence.
ACCESSION   DBNGZE010000192 DBNGZE010000000
VERSION     DBNGZE010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1695184"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1613
                     /gene="cysG"
                     /locus_tag="AC16FV_04990"
     CDS             216..1613
                     /gene="cysG"
                     /locus_tag="AC16FV_04990"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HIR2849451.1"
                     /translation="MYFLPIFLNLKKRPCVVIGGGEVASRKVSLLLDAGAEITVYAPD
                     LGETIRRWAGEGKIRHVTQEFRPEILDGCVLVIAATDDRAVNQRVSEAAKVRGIPVNV
                     VDQPDLCTFIMPSIIDRSPVMIAVSTGGRSPVLARLIRARLETLIPSGYGRLAKLVAT
                     FRDKVKQTFKHPPHRRQFWEEVLQGRIAELVYSGQDKAAEKALQERIDSADGDKTPIG
                     EVYLVGGGPGDPDLLTFRALRLMQQADVVVYDRLVSPAVLELVRRDAERIYAGKERDN
                     HAIPQEDINHLLVRLAKEGKCVVRLKGGDPFIFGRGGEEIDTLAAEGIPFQVVPGITA
                     AAGCAAYAGIPLTHRDYAQSVVFVTGHLQDGTVNLNWKALVQPDQTIVFYMGLHGIDT
                     ICRELIRHGMPPTTLAALVQQGTTRNQRVLTADLTTLPSIVEKAQVKAPTLIIVGDVV
                     ELHGRLKWFEPTAAN"
     gene            complement(1610..2278)
                     /locus_tag="AC16FV_04995"
     CDS             complement(1610..2278)
                     /locus_tag="AC16FV_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2849452.1"
                     /translation="MRVLLIDDHALVRKGLEELLHSRGVEVVASVGSGEEGIKQALAL
                     KPDVILLDIKMPGMNGIETLEKLRASNVTVPILMLTMSRDDADLRAALRGGAEGYLLK
                     DMDPEDLVPALEAALRGDNVVAKEMVGTLARIVQGQTNEPPPRSAAPLSDLTPREMEI
                     LSHVAEGQSNKMIARDLNITDGTVKLHVKAILRKLGVHSRVEAAVIAVEQGLGRKKTQ
                     SNEG"
BASE COUNT          419 a          684 c          717 g          483 t
ORIGIN      
        1 tggacgttca cgacaattct gccgggagca gaattggaca gtggagcgaa gcgacactgg
       61 ccctgcgtgc aaagcacgca gggcgagccc cagggatggg gcgaacaata ctacgcctca
      121 cccctccgcc aattttattt tctaccactt tacggatatc cgtaacggga tagaactccg
      181 cacggtgttt gggagaatcc gggtataatt tgtccatgta ttttctcccc attttcctaa
      241 atctcaagaa gcgtccctgt gtcgtcatcg gcggcgggga ggtggcgtcg cgcaaggtat
      301 ccttgctgct ggatgccgga gccgagatca ccgtgtatgc cccggatttg ggtgaaacga
      361 tccgacgttg ggcaggcgag ggaaaaatca ggcatgtcac gcaggagttc cgtccggaga
      421 ttctggacgg gtgcgtgctc gtgatcgcgg cgacggatga ccgcgccgtc aaccagcggg
      481 tttcggaagc cgccaaggtg cgcggcattc cggtcaatgt cgtggaccag cccgatctgt
      541 gcacattcat catgccgtcc atcatcgatc gttccccggt catgatcgcg gtttccaccg
      601 gtgggcgttc ccctgtgttg gcgcgcctga ttcgtgcgcg cctcgagacg ctgatccctt
      661 ccggttatgg aaggttggcc aagctggtgg cgacatttcg tgacaaggtc aagcagacgt
      721 tcaagcatcc accgcatcgc cggcagttct gggaagaggt gctgcaaggc cgcatcgccg
      781 agctggttta ttccggacag gacaaggctg ccgagaaggc gctacaagag agaatcgatt
      841 ccgccgacgg cgacaagaca ccgatcggcg aggtgtatct ggttggcggc gggccgggtg
      901 atcccgatct gctgacattt cgcgccttgc gtctgatgca gcaagcggac gtggtcgtat
      961 acgaccggct ggtgtcgccg gcggtgttgg agctggtgcg gcgtgacgcc gagcgtatat
     1021 acgccggcaa ggaacgcgac aatcacgcga tcccgcagga agacatcaat catctgctgg
     1081 tgcgcctcgc gaaggaaggt aaatgcgtgg tgcggctcaa gggcggcgat cccttcatct
     1141 tcggacgtgg tggcgaagag atcgatacgc tcgccgcgga aggcattccg tttcaggtgg
     1201 ttcccggcat taccgccgcc gctggctgcg ccgcttatgc cggcattccg ctcacacacc
     1261 gcgactacgc gcagtcggtc gtgttcgtta ccggtcattt gcaggacggg actgtaaacc
     1321 tcaactggaa ggcgctggtg caaccggatc agaccatcgt gttctacatg ggtttgcacg
     1381 gcatcgatac catctgccgc gagctgatcc gccacggtat gccaccgacc accttggcgg
     1441 cgctggtgca gcagggcacg acacgcaacc agcgcgtatt gaccgccgac ctgacaacct
     1501 tgcccagcat cgtggagaag gcgcaggtaa aagcgccgac attgattatc gtgggagacg
     1561 tggtggaatt gcacggtcgg ctcaagtggt tcgagccaac cgccgcgaat taaccttcgt
     1621 tgctttgtgt ttttttccgg cccagaccct gttcaaccgc gataaccgcg gcctcgacgc
     1681 gcgaatgcac acccagcttg cgcagaatgg ccttgacgtg gagtttgacg gtgccgtcag
     1741 tgatgttgag gtcgcgcgcg atcatcttgt tgctctgacc ctcggcgacg tggctcagaa
     1801 tctccatttc gcgcggcgtg aggtctgaca gcggcgccgc ggagcgtggc ggtggctcgt
     1861 tcgtctgtcc ttgcacgatg cgcgccaggg ttccgaccat ttccttggct accacattgt
     1921 cgccgcgcag ggcggcctcc agcgccggca ccagatcctc ggggtccatg tccttgagca
     1981 ggtaaccttc ggccccgccg cgcagggcgg cacgcaggtc ggcgtcgtcc cggctcatgg
     2041 tcagcatgag tatgggcacc gtgacattgc tggcccgcaa tttttccagg gtctcgatgc
     2101 cgttcatgcc cggcattttg atatccagca gaatgacgtc cggcttgagc gctagggcct
     2161 gtttgatgcc ttcctcgccg ctgcccacgg atgcgaccac ctccacgccc cggctgtgca
     2221 gcagttcctc gaggcccttg cgaaccaggg catggtcatc gatcagcaga acgcgcaatc
     2281 cggtttcctc ttgttctctg tta
//
LOCUS       DBNGZE010000193         4239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_305740, whole genome shotgun sequence.
ACCESSION   DBNGZE010000193 DBNGZE010000000
VERSION     DBNGZE010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_305740"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="AC16FV_05000"
     CDS             complement(<1..282)
                     /locus_tag="AC16FV_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR2849453.1"
                     /translation="MLVKEIMTRKVESIPPTATLDKAAQKMRDLKLGSLPVMVSGKLI
                     GMITDRDICCRGVASGLEPATTAVSEIMSRDIRFCFSDDTVTAAVRQMEE"
     gene            complement(419..1342)
                     /locus_tag="AC16FV_05005"
     CDS             complement(419..1342)
                     /locus_tag="AC16FV_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR2849454.1"
                     /translation="MPAYLALLERGELRSREQQAWWHLENCDLCARYCHVNRRETTKG
                     AVCRTGERAVVNSYGPHHGEEDPLRGVNGSGTIFFSWCSLRCVFCQNWDISQKGIGNE
                     VEPAELAGMMLRLQSLGCHNINFVTPSHVVAQIIAAVRIAAEQGLRLPLVYNTGGYDS
                     PEALHLLDGIIDIYMPDMKYGDSRLARQYSKVRDYVEINQAAVKEMHRQVGDLVLDER
                     GIAQRGLLVRHLVLPVGIAGTEKVLAFLSREISRNTYLNLMDQYRPCYRVDEYPPLDW
                     PVTGREFQDALQVAERYGLYRLDHRRSRYGL"
     gene            1606..1842
                     /locus_tag="AC16FV_05010"
     CDS             1606..1842
                     /locus_tag="AC16FV_05010"
                     /inference="COORDINATES: protein motif:HMM:NF042799.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HIR2849455.1"
                     /translation="MDETPIEGCWYETETGEVFMVVKVDTASGLADVQYLNGRPDQIE
                     RAIWNGLDLTHVEPPDEWRADMDTFFHERGRKKH"
     gene            1954..3939
                     /locus_tag="AC16FV_05015"
     CDS             1954..3939
                     /locus_tag="AC16FV_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HIR2849456.1"
                     /translation="MNHSMRREFSWFDNQPDELIRRLLADDRRVLLMGSPGIGKSCLA
                     AQLARLLAQGGRPCSCLSADPGSPAFGVPGAVTLGIWDSLRWRVVAAEALCTLDAGRF
                     RLPLVAAVQRLAAQVPAMTLLVDAPGAVRGVTGSELMQGLLAATAADLILVLARDEAS
                     VPLHDELRAQSAEVALVRAASLAQRPGKKARARTRTAAWDAYLHKPVEQTLTLPTLQI
                     VGTPPPLTVPSAWIGRQVALSDSTGVQVLGEVRAMTGQSLRLMLPRPLHAPRVLLVRD
                     AQRSLSGSLETAAVYAAEPLDYLPPPDVTPFARMVAAGGPWVTGEVGGLSVILVNGVF
                     GDPLLYVRLRHQRRNLLFDLGASVRLAARVAHLVTDVFISHTHLDHIGGFFWLLRARI
                     GEFPPCRLYGPPGLAQHIEALIRGILWDRVAERGPFFEIAELHDRRLRHYAIQAGQGG
                     LKFCGEEEAMGSVIRQEHGFRVRAVTLDHHTPVLAYAFEPAQQINIRKDRLQARRWTP
                     GPWLTELKQRLMAREYETDIHLPDGSCIAAGVLADDLTLIQPGKKLVYATDFADTSQN
                     RERLVHLAQGAHTFFCEASFVEADAALAARTGHLTTRACGEIASAAAVTRLVPFHFSL
                     RNRDEPDTVYDEIRAACPQVALPRHMRVFATTLPTDK"
     gene            complement(3995..>4239)
                     /locus_tag="AC16FV_05020"
     CDS             complement(3995..>4239)
                     /locus_tag="AC16FV_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849457.1"
                     /translation="QQQQTDRQRLEQHYRQQLQTPAPGRSQEQIRRQEQSEMKLLREE
                     HRREQRLLERRQQREYPAVREPRKDDKSNERRWDTR"
BASE COUNT          749 a         1283 c         1353 g          854 t
ORIGIN      
        1 ttcctccatc tggcgcacgg cggcagtcac ggtgtcgtcg ctaaaacaga accggatgtc
       61 acgcgacatg atctcgctca ccgcggtggt ggccggctcc aaaccgctgg ccacgccccg
      121 gcaacagata tcgcgatccg tgatcatgcc gatcagcttg ccgctcacca ttaccggcag
      181 cgagccgagt ttcagatcgc gcatcttctg cgcggccttg tccaacgtcg ccgtcggcgg
      241 gatggattcc accttgcgcg tcatgatttc ctttaccagc atggctacct ccttttgcag
      301 cccggtgctc gcacacatat cgtgaatatt caaatccaaa atcagcgatg gtgtcgttga
      361 tagtgatcaa aatgctgaag cattagccgg gtttatgacc ggcgcacagc ggttccggtt
      421 acaacccgta gcggctgcgg cggtggtcga gacgatacag tccgtaccgc tccgcgacct
      481 gtaacgcatc ctggaattcc cgtcccgtga cgggccaatc gagcggcgga tattcgtcca
      541 cgcggtaaca gggacggtac tggtccataa ggttgagata ggtgttgcgc gatatctccc
      601 gcgacaggaa ggccagcacc ttttccgtgc cggcgatgcc caccggcaac accagatgcc
      661 gcaccagcag gccacgttgc gcgatgccgc gctcgtccag caccagatcg ccaacctggc
      721 ggtgcatctc ctttacggct gcctgattga tctcgacgta gtcacgcact ttcgaatatt
      781 ggcgcgccag ccgcgaatca ccgtatttca tgtccggcat gtagatgtcg atgatgccat
      841 cgaggagatg cagggcctcc gggctgtcgt agcctccggt gttgtacacc agcggcaggc
      901 gcagtccctg ttcggcggca atgcgtaccg cggcgatgat ctgggccacg acgtggctgg
      961 gggtgacgaa gttgatattg tgacagccga gtgattgcag acgcagcatc atgccggcca
     1021 gctccgccgg ctcgacctca ttgccgatgc ctttttggct gatgtcccag ttctggcaga
     1081 acacgcagcg caggctgcac cagctgaaaa aaatcgtgcc cgatccgttc acgccgcgca
     1141 gcggatcttc ctcgccgtgg tgcggaccgt aactgttcac gacggcgcgc tcgccggtgc
     1201 ggcacacggc gcctttcgtg gtttcacgac gattgacgtg gcaatagcgc gcgcacagat
     1261 cgcaattttc caggtgccac caagcctgct gttcacggct gcgcagctcg ccgcgttcca
     1321 gcagggcgag gtaggccggc ataaacgtgc cggcggcaga ttggggagag ggctttgccg
     1381 gcatggtgga cgaaatagcg tgtgcaacga ttctgcgcct cggcaacgca caaaacaatg
     1441 tcttgcgctg catcggcggc acggcgaaga cacgccggaa tagggcgttt tgcgaatcgt
     1501 tgaccgcgat caacgttcgg cccgcgagac tggctgaaga ttgagcttcg ggaaatccgg
     1561 cgtcgtgctt gcgctggacg cagtttttca catggaggaa caactatgga tgaaacacca
     1621 atcgaaggct gctggtatga aaccgagacc ggcgaggtgt ttatggtggt gaaggtcgat
     1681 acggcgagcg gcctggccga tgtgcagtac ctgaatggca ggcccgacca aatcgagcgc
     1741 gcgatctgga atggtctcga tctcacccat gtcgagccac ccgacgaatg gcgcgcggac
     1801 atggacacgt tcttccacga acgcgggcgc aagaagcact gagccggttt cagttctgtc
     1861 atgtcggcgt acacgctgtg ccaataacct ttggcgcagg cgatcctcgg acttttgatg
     1921 ctcgggagaa acggtcttct ctgtgtgaat gaaatgaacc attccatgcg gcgagagttt
     1981 tcctggttcg acaatcagcc tgacgaactc atcagacgcc tgttggcgga cgaccggcgg
     2041 gtgctgctga tgggttcacc gggaattgga aaatcctgtc tggccgcgca actggcacgg
     2101 ctgttggcgc agggcgggcg gccatgcagt tgtctgagcg ccgatcccgg ttccccggcg
     2161 ttcggcgtgc cgggtgcagt aacgcttggc atctgggatt ctttgcggtg gcgtgtcgtc
     2221 gcggctgaag ccttgtgcac gctcgatgcc ggacgtttcc gcctgcccct ggtcgcggcg
     2281 gtgcaacgac tggccgcgca ggtgcccgcg atgaccttgc tggtggacgc gcccggtgcg
     2341 gtgcgcggtg tcaccggctc cgaattgatg caggggctgc tggccgcgac cgccgccgac
     2401 ctgatcctgg tgttggcgcg cgatgaggcc tccgtgccgt tgcacgatga gctgcgtgcg
     2461 caatcggcgg aggtggcatt ggtgcgcgcg gcatcactcg cacagcgacc cggaaaaaag
     2521 gcgcgggcgc gtacgcgcac agccgcctgg gacgcctacc tgcacaagcc ggtcgaacag
     2581 accctcacgc tgccgacgct gcagatcgtc ggtacgccac cgccactgac agttccctca
     2641 gcctggattg ggcggcaggt ggccttgtcc gacagcacgg gagtccaggt gttaggcgaa
     2701 gtgagagcca tgacagggca aagcttgcgg ctgatgttgc cgcgtccgct gcacgcgccg
     2761 cgagtgttgc tggttcgcga tgcccagcgt tcgctgtccg gcagtctgga gacggcggct
     2821 gtgtatgcgg ccgaaccgct ggattacctg ccgccaccgg atgtcacacc cttcgcgcgc
     2881 atggttgccg ccggaggacc ctgggtcacc ggagaggtgg gcggtctcag cgtcatactg
     2941 gtcaatggcg tgttcgggga tccgctgctg tatgtgcggt tgcggcatca gcgccgcaac
     3001 ctgctgttcg atctgggcgc gagcgtgagg ctggcggcac gcgtcgcaca cctcgtcacc
     3061 gacgttttca tcagtcatac ccatctggat catatcggtg gttttttctg gctgctgcgc
     3121 gcccggatcg gcgagtttcc gccatgccgg ctgtacggac caccgggact ggcgcagcat
     3181 atcgaagcgc tgatccgggg tattttgtgg gatcgtgttg ccgagcgtgg cccgtttttc
     3241 gaaattgccg aactgcatga ccggcggctg cggcactatg ccatccaggc aggccagggc
     3301 ggattaaagt tctgcggcga agaagaggcc atgggctctg ttatccgcca ggaacacggt
     3361 ttccgggtcc gcgcggtgac cctcgatcat cacacaccgg tgctggccta tgccttcgag
     3421 ccggcgcaac agatcaatat ccgcaaggac aggctgcagg cgcggcgctg gacgcccggg
     3481 ccgtggctga cggagttgaa gcaacgactc atggcgcgcg aatatgaaac ggatattcat
     3541 ctgccggatg gcagctgcat tgccgccggc gtgctggcgg acgatctgac cctgatacag
     3601 ccgggcaaaa agctggtcta tgcgaccgac ttcgccgata cctcgcagaa ccgggaacgc
     3661 ctggtgcatc ttgcccaagg ggctcatact tttttctgcg aggcgtcctt cgtggaagcg
     3721 gatgccgcgc tggccgcgcg aaccggtcac ctgaccaccc gtgcctgcgg ggagattgcc
     3781 agcgccgctg ccgtgacgcg cctggtaccg tttcatttct cgctgcgcaa ccgcgacgaa
     3841 ccagacacgg tctatgacga aatccgggcc gcctgtcccc aagtggcgtt accgaggcat
     3901 atgcgggtct tcgccacgac cttgccgact gataaatgaa tcaaggggct tcgccattcg
     3961 gcggcagggc atccgcggga ttccaccacg gcaactatcg cgtgtcccag cggcgctcgt
     4021 tggatttgtc atccttgcgc ggttctcgca ccgctggata ttctcgttgc tggcgccgct
     4081 ccagcaaacg ctgctcgcga cgatgctcct cgcggagcag cttcatttcc gattgctcct
     4141 gccggcgaat ttgctcctgg gatcgccccg gagccggggt ctggagttgc tggcgataat
     4201 gctgctcgag ccgttgccgg tcggtttgtt gttgctgga
//
LOCUS       DBNGZE010000194         7930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_306041, whole genome shotgun sequence.
ACCESSION   DBNGZE010000194 DBNGZE010000000
VERSION     DBNGZE010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_306041"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1024)
                     /locus_tag="AC16FV_05025"
                     /pseudo
     CDS             complement(221..1024)
                     /locus_tag="AC16FV_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392719.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(1168..1683)
                     /locus_tag="AC16FV_05030"
     CDS             complement(1168..1683)
                     /locus_tag="AC16FV_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849458.1"
                     /translation="MQKIFALFFLFIPFTSLAEIESSPARALILSVGVSSTSSKPFNP
                     LGEYENKSNQYSLRYEGRRFGLRLDHSIHGDDDGNTYITGDVLARPWGPIVLGAGLSY
                     AKQPLRGIGRQANLHAMFGVEDPTMFGPRTGAGAWFDHWSNGHIHQIIGWDDVPNPPR
                     NAISGGIIHRF"
     gene            complement(1720..2559)
                     /locus_tag="AC16FV_05035"
     CDS             complement(1720..2559)
                     /locus_tag="AC16FV_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849459.1"
                     /translation="MPKFVQPDYATQTGTAYPLNLDADMKVLSRMGAAYNPCAEDNPT
                     ALKVVVQAGAMFVNGALVANAAQTVTLAAAHATLPRIDRVVIDAVTGVASKIDGTAAA
                     SPTPPAITAGKLPVAQVLVTALMTVVTNNAITDERAIVFDAMAAAMARPAASGYTRIA
                     PNFCSRPLGTFQLLSTGVCTAVPLPDPAAKMVEVAIDVWTLSGNAVASRTAQVDSHNN
                     IVCSGGMVDRAYLAGYEFVATPGGILLDEDYQRIKAPPVAGNIYLNKVSGAAGYYITG
                     YWD"
     gene            complement(2563..6426)
                     /locus_tag="AC16FV_05040"
     CDS             complement(2563..6426)
                     /locus_tag="AC16FV_05040"
                     /inference="COORDINATES: protein motif:HMM:NF040662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HIR2849460.1"
                     /translation="MEIAPNLVYSPHPLLAAANRKLLYAPFVPGESLAIWLSRLGITI
                     GAHPVILALNDRIVPRDQWGAAFPQEGDIVTIRATIQGGGEGGSNPLRTVLTIALLIA
                     APQAGAFLAWGTGVPAAVGTALTIVGGTLIIDRLAPLPKPPRPGESPAASPTYSLAGA
                     GNRARPYEPLPLVLGTHRIYPDLGAKPYTEFRGNDQYLYLVFNFGLSDVVLSDFKIGD
                     TPIADFDGVEIQESGPDGALTLFPANVDTLAGGALTAAAGWITKTSSTAATALAVDIS
                     GLLFYSGNDGLQPRGVNLEIEYRAVGAGTWVPFLDSPSFGIPTWSANTYIGTARRNIT
                     GSGAIAIPTTFNGHYYRRTTRIQGTGEVGDQSYTYTGSSEPSWPLTSGTFVQDGSAFS
                     TGGDDPVTLVPGWEEVGTLSASNVYIQNGSRAPVRKTYRRNVPSGQYEVRVRRISGDE
                     TDDRAVSDMTWESLKTYQPDTTDYTGQKRIAIIVKASGQLQGALDTFNAIASARTLAW
                     NGSAWVLQATSNPAWWWLWFARGKSIGGRRAFGALLPDSRLDLEAIKAFGAYCTSKNL
                     TFDAVLDRVTNCREVLDLIARCGRGATTTATGKFGAVWDAANQPVVQVFGMGNIKRNS
                     FQVEYITGKLADEIVVNFINPALNWQQDTVRVPVAGALNPSRPAVVDLFGCKNKDMAG
                     AEANLIAAAQVWRRRRISWESDFEGMVSQRGDVVVLSHDLTQWGYSGRLLGGNGVTLQ
                     LERKVPFTPLTQHYIGITFPNGYYQIFDVQIAVGETDTIILTEAWPAVDDLGNALYTP
                     DTDPNHPPFDYKFVFDPKATPGKKVKILDIKPLSESYVRLTATDEEPTYYAAESASYT
                     YAPPAVFEAALPTITNVTITDTLIVVGGGYATRIAIAFNVSGTYGFATVRAGLAGMPL
                     QTVGQTDERRFEFTAPPSGVMDIEITARAPSYHFKAAGRYTTQYVILGKDRLPQNVQN
                     FSGSQNGRFIIYRWDEVSDVDRDGYEIRRAPAGIADTAEAWRRGVLVVEAEQGTEMTT
                     GVVPPGNWTHLIKAKDTSGNFSATAARFDLEVTNALDIIQQKQQAPDWLGTKTNFLVH
                     WTGKLVPESTKLASQHTREELFEQFVPYPYATCSYEATEIDLGFDSDARVWADIQAQL
                     GRGRSGIVDTPLSIDYRLAAGAYDGFEPWSIGDVRARYIKQKFTLDTSLGCCYVSSMN
                     AVVDSEEFDQSGTFISAIGGVTITFPEPFHVLSDFQITPEGAATAYTATYENLSTTGA
                     KGHVWRVSDATEIAGVNARWSAKGV"
     gene            complement(6417..6797)
                     /locus_tag="AC16FV_05045"
     CDS             complement(6417..6797)
                     /locus_tag="AC16FV_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849461.1"
                     /translation="MHWTQQYIGMPYIEGEQDCGEFAARIQREVFRREVRLPTERAAG
                     LRGKSRQIESLLDDYGLVTDAPVDGDAVLMKGGSLWHIGVYALIGGIPYVLHAMKNAG
                     QVVLHRLRDLENQGLKFEGFYKWK"
     gene            complement(6841..7317)
                     /locus_tag="AC16FV_05050"
     CDS             complement(6841..7317)
                     /locus_tag="AC16FV_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1833 family protein"
                     /protein_id="HIR2849462.1"
                     /translation="MPRNYSRSFQEKVNSTSANEAPLLLLEISHVDLVTPIRVVNDNQ
                     DLAHNGNTFVAMAFRITPPDDMQDGQSRAAIAVDNVGRELTQWLEMSGGGQGATVRII
                     QVLRSAPNTIEWEVTMDLADVRINQREVTGTLTFDDILNLPAVALVYRPDVAPGIY"
     gene            complement(7321..7674)
                     /locus_tag="AC16FV_05055"
     CDS             complement(7321..7674)
                     /locus_tag="AC16FV_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849463.1"
                     /translation="MIGYGKLLLRDYGERPESAVLRTEMESGFAKQAKIRSKVPVFRP
                     AVIDYSNTEYSQFKTWFKDTLARGTNWFAWTDPQDGVSKQARIVRGEYEARSYFKTEG
                     ETLSWEVSFLLETME"
     gene            complement(7671..>7930)
                     /locus_tag="AC16FV_05060"
     CDS             complement(7671..>7930)
                     /locus_tag="AC16FV_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849464.1"
                     /translation="LGIKRDEVPAILQEGETVLPRGARLGGTDKVSVIVENRGQPIKA
                     TDTKVDVDVEGTVVRVLVEDAQRGGPVSSTLARTFSLKRGG"
BASE COUNT         1499 a         2370 c         2461 g         1600 t
ORIGIN      
        1 gtttgtcttg ggacagaggg tgcaagagct atgccacccg ttctaaagct taagaatcaa
       61 caggttgtcc gaaggacggg atcgattcac caagatgaaa ttggactgaa acgggcgctt
      121 taatgactgt aaaccgacac ttggtttgcg attttccgac acttggtttg cgacgcccaa
      181 aatcggggta aaaagggtac ttaactcacc caaaatcgac ctaaaagagg ctcaaagtac
      241 ccgtttcgcg ccggcggtcc tcgcagtgct ggttccagat cagcagctcc cgggccgggg
      301 tgccggcgcc gccgccgacg gtgtaattga tggccagctc atctatatat aggccgtcga
      361 acacccggcg gatatctggg tggtcgttga tgctgatcag gaccgatccc tggactgtcc
      421 tggccagctc ggccatgcgc tcgtactgct cgaacccgaa ctccaccccg tagccctcgg
      481 tttgccagta gggcgggtcg aggtagaaca gagtgtgcgg ccggtcgtag cgagcgatga
      541 tctccggcca cggcagattc tcgacctaga cgccatgcag ccgcagatgc gcctggctca
      601 ggtcctcttc caaccgcagg aggttgaggc gcgggccgct ggtggtcgcg gtgccgtagc
      661 tctggccgtc cacccgcccg ccgaaggcgt gcttctgcag gtaatagaac cgcgcggcgc
      721 gctggatgtc ggtcagggtt tccggggcct ttttctgctc ccattcgaag acctggcggg
      781 agcttaaggc ccatttaaac tgccgcacaa actcctctaa atggtgctgc acgacccggt
      841 agagattcac cagctcgccg ttgatgtcgt tcagcacctc gacgtcagac ggctccttct
      901 cgaagaagat cgcggccgcg ccgcagaagg cctcgacgta acaggtgtgg gccgggaacc
      961 gatcgaagat cggcttggcc agacggcgct tcccaccgat ccacggaata ataggtgtgc
     1021 ccatcgtgag cctccgttct ttttttcgtg tactagactc caagtgctgc gtacgcggca
     1081 ggaggtgcct agcctggctc acagtgctca tcactgtgga tcgggggccg ggccgtgcgc
     1141 gaacacggtc cggtcgccct ctttcattca gaacctatgg atgatgcctc ccgagatcgc
     1201 gttccgcgga ggattcggca catcgtccca tccgatgatc tgatggatat ggccgttcga
     1261 ccaatgatcg aaccaggcac cggcaccggt gcgcggaccg aacatcgtcg ggtcttcgac
     1321 gccgaacatc gcatgcaggt tcgcttgccg gccgatgccg cgcagcggct gcttcgcgta
     1381 actcaagccg gcgcccagga cgatcggtcc ccacggccgc gccagcacgt cgcccgtgat
     1441 gtaggtattg ccatcgtcgt caccgtggat gctgtgatcg agacgcaggc cgaatcgccg
     1501 gccctcgtag cgcaggctgt attggttgct cttgttttca tactcaccca gcgggttgaa
     1561 cggtttcgag ctggtggagc tgacgccgac cgataatatg agcgcacggg ctggtgaaga
     1621 ttcgatctcg gccaacgatg tgaagggaat aaatagaaaa aacagagcaa atattttttg
     1681 catttaatct cctttattgc atgaaaagat tttcaatcat taatcccagt atccggtgat
     1741 gtagtaacca gctgctccgg aaactttatt caaataaatg tttccggcga ccggcggcgc
     1801 cttgatgcgc tggtaatcct catctaaaag aattcctccc ggggttgcga caaattcgta
     1861 ccccgccaga taggctctat ccaccattcc acccgagcat acgatattgt tgtgactatc
     1921 cacttgagct gttcggctgg cgacggcatt gcccgaaagc gtccatacat ctatggcgac
     1981 ttcaaccatc ttggcggcag gatcggggag cggaacagca gtgcaaacgc ctgtagataa
     2041 taattgaaat gttcccaagg gacggctaca gaaattcgga gcaatgcgcg tgtaaccgga
     2101 tgcggcggga cgggccatcg ccgccgccat cgcatcaaac acaatcgctc tttcatccgt
     2161 gatggcatta tttgtaacca ccgtcatcaa cgctgtcacc agaacctgcg ccaccggaag
     2221 ctttcctgcg gtgatggcag ggggtgtcgg tgaggcagcg gcggttccgt caatcttgct
     2281 ggcaaccccc gtcaccgcat caatcaccac gcgatcaatg cgcggcaagg tggcatgcgc
     2341 cgcggcgagc gtcaccgtct gcgcggcatt ggccaccagc gcgccgttga cgaacatggc
     2401 gccggcctgg acgactacct tgagggcggt gggattgtcc tcggcgcaag gattgtaggc
     2461 cgcccccatg cgagacagga ctttcatatc ggcatcgagg ttcagcggat aggcggtgcc
     2521 ggtctgggtg gcgtagtcgg gctgcacgaa tttaggcatc gttcaaactc ctttggctga
     2581 ccagcgcgcg ttcacgccgg cgatttcggt ggcatcggac acgcgccaga cgtggccctt
     2641 ggcgcccgtg gtgctgagat tttcataagt cgcggtatag gcggtggcag caccctccgg
     2701 cgtgatttgg aaatcgctga ggacatggaa tggctctggg aacgtgatcg tgacaccgcc
     2761 gatcgcggag ataaaggtgc cggattgatc gaactcctcg gaatccacca cggcattcat
     2821 actgctgacg taacaacagc cgagtgaggt atcgagcgtg aatttctgct tgatgtagcg
     2881 ggcgcggacg tcgccgatgc tccacggctc aaagccatcg taggcgccgg cggccagccg
     2941 gtaatcgatc gagagcggcg tgtcgacgat gccgctgcgg ccacggccga gctgcgcctg
     3001 gatatcggcc cacacgcgcg catcggaatc gaagcctaaa tcgatctccg tggcctcgta
     3061 gctgcaagtc gcgtagggat aaggcacgaa ctgctcgaac agttcctcgc gggtatgctg
     3121 gctcgcgagc ttggtgcttt cgggcaccag cttgccagtc caatgcacca aaaagttggt
     3181 cttggtgccg agccaatccg gcgcctgctg tttctgctgg atgatatcga gcgcgttggt
     3241 gacttcgaga tcgaagcgcg cggcggtcgc gctgaaattt ccgctcgtgt ccttggcctt
     3301 gatcagatgc gtccagttgc ccggcggcac cacgccggtg gtcatctccg tgccttgctc
     3361 ggcctcgacc acgagcacgc cacggcgcca agcttcggcg gtgtcggcga tcccggccgg
     3421 agcgcggcgg atttcgtagc cgtcgcgatc cacgtcggac acttcgtccc agcgatagat
     3481 gatgaagcgg ccgttctgcg agcccgagaa gttctgcacg ttttgcggca ggcgatcctt
     3541 gccgaggatg acgtattgcg tggtgtagcg gccggcggcc ttgaaatgat acgacggcgc
     3601 gcgtgcggtg atctcgatat ccatcacgcc cgagggcggc gcagtgaact cgaagcggcg
     3661 ctcatcggtc tgccccacag tttgcagcgg catgccggcg agaccggcgc gcacggtggc
     3721 gaagccataa gtgccgctga cattgaaggc gatcgcaatg cgcgtagcat aaccaccgcc
     3781 gaccacgatc agggtgtcgg tgatggtgac gttcgtgatg gtgggcagcg cggcctcgaa
     3841 caccgccggc ggcgcgtagg tgtagctggc cgattcggcg gcgtaataag tcggttcttc
     3901 gtcggtcgcg gtcaggcgca catacgattc ggagagcggt ttgatgtcca ggattttgac
     3961 tttcttgccc ggcgtggcct tgggatcgaa cacgaacttg tagtcgaacg gcgggtgatt
     4021 cggatcggtg tccggcgtat agagagcgtt ccccaagtca tcgaccgctg gccaggcttc
     4081 ggtgaggata atcgtgtcgg tctcgccgac cgcgatctgc acgtcgaaaa tctggtaata
     4141 gccgttcggg aaggtgatgc cgatgtagtg ctgagtgagc ggcgtgaagg gcaccttgcg
     4201 ctccagctgc agcgtcacac cattgccgcc caggagccgc ccgctgtaac cccactgcgt
     4261 gagatcgtgc gagagcacca cgacgtcgcc gcgctgggag accatcccct caaagtcgga
     4321 ttcccaagag atgcggcggc ggcgccacac ctgcgccgcg gcgatgagat tcgcctccgc
     4381 gccggccata tccttgttct tgcagccgaa cagatccacc accgccgggc gcgacgggtt
     4441 gagcgcgccg gccacgggca cgcgcaccgt atcctgctgc cagttgagcg cgggattgat
     4501 gaaattcact acgatctcat cggcgagctt gccggtgatg tattccacct ggaagctgtt
     4561 gcgcttgatg ttgcccatgc cgaatacctg caccaccggc tggttggcgg catcccagac
     4621 tgcgccgaat ttgcccgtgg cggtagtcgt ggcgccgcgc ccgcaccgcg cgatgaggtc
     4681 caggacctca cgacaattcg tcacgcgatc gagcacagca tcgaaggtaa gattcttgct
     4741 ggtgcaatag gcgccgaaag ctttgatggc ttcgaggtcc aggcggctat cgggcagcag
     4801 cgcaccgaac gcgcgccggc cgccgatgct cttgccgcgc gcgaaccaca gccaccacca
     4861 agcgggattg ctcgtggctt ggagcaccca ggccgagccg ttccatgcca gcgtgcgcgc
     4921 cgaggcgatg gcattgaagg tgtcgagcgc accctggagc tggccgctcg ccttgacgat
     4981 gatcgcgata cgcttctgtc cggtgtaatc ggtggtgtcc ggctgatagg tcttgagcga
     5041 ctcccaggtc atgtcgctca cggcgcgatc gtcggtttca tctccgctga tgcgccgcac
     5101 gcgcacttcg tattgccctg aaggaacatt gcgtcggtag gttttacgga ccggcgcgcg
     5161 cgagccgttc tgaatgtata cattcgaagc gctgagcgtg ccaacttctt cccagccagg
     5221 gacgagagtc accggatcgt caccaccggt actgaacgca cttccgtctt gcacaaaagt
     5281 gccggaggtc agcggccaag atggttccga tgaaccggta taggtataac tctggtcgcc
     5341 gacctcgccg gtgccctgta tgcgcgtggt gcgccgataa taatgcccgt tgaacgtcgt
     5401 cgggatcgcg atggcgcctg agccggtgat gttccgacgt gcggtgccga tataggtatt
     5461 ggccgaccaa gtcggaatgc caaagctggg cgaatcaagg aatggcaccc aagtgccggc
     5521 accgacggcg cggtactcaa tttcgaggtt cacgccgcgt ggctgtagac cgtcattgcc
     5581 ggaataaaac agcagaccgc tgatgtccac cgcgagcgcg gtggcggcgg tgctcgaggt
     5641 cttggtgatc cagccggcag cggctgtgag cgcgccgccg gcgagggtgt ctacgttcgc
     5701 cggaaatagc gtgagcgccc catcggggcc ggattcctgt atttccacac catcgaaatc
     5761 cgcaatcggt gtgtcgccga ttttaaagtc cgacagcacc acgtctgaca gaccgaagtt
     5821 gaacaccaga taaagatact gatcgttgcc gcggaactcg gtgtacggtt tcgcgccgag
     5881 atccgggtag atgcggtgcg tgcccagcac cagcggcagc ggctcatagg gccgtgcgcg
     5941 gttgccggca ccggcaagcg aataggttgg gcttgcggcc ggcgattcgc cggggcgcgg
     6001 cggtttcggc agcggcgcaa gacggtcgat gatcaaagtg ccgccaacga tggtcagggc
     6061 ggtaccaacg gccgccggca ctccagtgcc ccaggccaga aacgcgccag cttgtggcgc
     6121 cgcgatcagc aacgcaatgg tgaggaccgt ccgcagcgga ttgctgccac cctcaccgcc
     6181 gccctgaatg gtggcacgga tggtgacgat atcaccttct tgtggaaagg ctgcacccca
     6241 ttgatcacgc ggcacgatgc ggtcgttgag cgcgagaatt acgggatgcg ctccgatagt
     6301 aattccgagg cgcgagagcc agatcgcgag cgattcgcct ggcacgaacg gtgcgtagag
     6361 cagtttgcgg ttcgcggcgg cgagcagcgg gtgcggactg tagaccaggt tcggggctat
     6421 ttccatttat aaaatccctc gaacttcagt ccctgattct cgagatcgcg caggcggtgc
     6481 aagacgacct ggccggcgtt cttcatcgca tgcagcacgt agggtatacc gccgatgagg
     6541 gcatagactc cgatgtgcca caggctgccg cccttcatca gcacggcgtc gccgtccacg
     6601 ggcgcgtcgg tgaccagacc gtaatcgtcc agcagtgatt cgatctggcg cgatttgccg
     6661 cgcagcccgg cggcgcgctc ggtcggcagg cggacttcgc ggcggaatac ctcacgctgg
     6721 atgcgagcgg caaactcgcc gcagtcctgt tctccctcga tgtagggcat gccgatgtac
     6781 tgctgcgtcc agtgcattaa tgtcgcccta cttcgctgcg ctgcgttcgt ctggccatat
     6841 ttaataaatc cccggcgcca cgtcaggccg atagaccagc gcgaccgcgg gcagattgag
     6901 gatgtcgtcg aaagtcagcg tgccggtcac ttcacgctgg ttgattcgca catcggcgag
     6961 atccatcgtc acttcccact cgatggtgtt cggggccgag cgcagtacct ggatgatgcg
     7021 cacggtcgcg ccctggccgc cgccactcat ctccagccac tgcgtcagct cacggccgac
     7081 gttgtccacc gcgatcgcgg cgcgcgactg gccatcctgc atgtcgtctg gcggcgtgat
     7141 gcggaaggcc atcgcgacga atgtattgcc gttgtgtgct aggtcctggt tgtcattgac
     7201 cacgcggatc ggcgtgacca ggtcgacgtg ggaaatctcc agcaatagca gtggagcttc
     7261 gttggccgat gtggaattta ctttttcctg gaacgatcgc gagtaattgc ggggcatgct
     7321 ttattccatc gtttccagca ggaaagacac ttcccagctc agcgtctcac cttcagtctt
     7381 gaagtagctg cgtgcctcgt attcgccacg cacgatgcgt gcctgcttgg agacgccatc
     7441 ctgcggatcg gtccaggcga accagttggt gccgcgcgcc agggtgtcct taaaccacgt
     7501 tttaaactgg ctgtattccg tgtttgagta gtcgatgacg gccggacgga agaccggaac
     7561 cttgctgcga atcttggcct gcttggcaaa gccggattcc atctcggtgc gcagcacggc
     7621 gctctccggc ctctcgccgt agtcgcgcag cagcaatttg ccgtaaccga tcatccgccc
     7681 ctcttcaagc tgaaagtgcg cgcgagcgtg ctggaaaccg gcccgcctcg ctgagcatcc
     7741 tcgacaagga ccctcaccac cgtgccttcc acgtcgacat cgacctttgt gtccgtggcc
     7801 ttgatcggct ggccgcgatt ctcgacgatg acgctgacct tgtcggtgcc gccaaggcgt
     7861 gcgccacgtg gcaataccgt ttcaccctct tgcaggatgg ccggaacttc atcgcgttta
     7921 atgccaagcg
//
LOCUS       DBNGZE010000195         5340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_444926, whole genome shotgun sequence.
ACCESSION   DBNGZE010000195 DBNGZE010000000
VERSION     DBNGZE010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_444926"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16FV_05065"
     CDS             complement(<1..473)
                     /locus_tag="AC16FV_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR2849465.1"
                     /translation="MFEKTVAELSRALGRKEVSSVELTRVFLDRINKYRSLNAFITVD
                     PERSLAQAKAADARIARGEAGALTGVPIAQKDIFCADGWLTTCGSKILSNFVAHYDAT
                     IIDKFNQAGAVCLGKTNMDEFAMGSSNETSYYGAVKNPWGTDRVPGGSSGGSAAAV"
     gene            complement(503..790)
                     /gene="gatC"
                     /locus_tag="AC16FV_05070"
     CDS             complement(503..790)
                     /gene="gatC"
                     /locus_tag="AC16FV_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR2849466.1"
                     /translation="MSLDREQVRKIAHLARLHITGQEADAYAETLSRILGLIEQMNAV
                     DTAGVAPMAHPNDAGLRLREDAVTEPDQREKFQKIAPAVEAGLYLVPKVIE"
     gene            917..1960
                     /locus_tag="AC16FV_05075"
     CDS             917..1960
                     /locus_tag="AC16FV_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR2849467.1"
                     /translation="MFKSLRGFFSSDIAIDLGTANTLIFVRDKGIVLNEPSVVAIRQD
                     YTSRNNRAILAVGHEAKKMLGRTPGSIQAIRPMKEGVIADFTVTGEMLKYFIRKVHEN
                     RMFQPSPRIVICVPCGSTQVERRAIREAAMSTGAREVFLIEEPMAAAIGADLPIMEPT
                     GSMVLDIGGGTSEVGVISLGGIVYSHSLRIAGDKMDESIVNYVRRKYGLLIGEASAEQ
                     VKKEIGTAWEGSEIKQVEVKGRHIADGVPRAQVLNSQEIHAALSECLAGIIGAIKIAL
                     EQTPPELGADIAEKGLVVSGGGALLRDIDRMLAEQTGLPIIITEDPLTCVVRGCGRAL
                     MEAETLGDVFVYE"
     gene            2029..2886
                     /gene="mreC"
                     /locus_tag="AC16FV_05080"
     CDS             2029..2886
                     /gene="mreC"
                     /locus_tag="AC16FV_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR2849468.1"
                     /translation="MINVFGRSIGAPSHLTRFVLLSALSVALMMLDHRGHHLEKIRAG
                     LNTLAYPIQIIAATPAYVVGGMADFFTTRGTLRVDNEKLLTERQALLAKLQQFEALEQ
                     ENQRLRQMLVSAAQVADKAIAAELVEVSAEPFTRKVVVAKGAQDGVYVGQSVIDAHGI
                     MGQVTQVAGNVSRVTLITDAGHAIPVLDNRSGLRALVFGTGNPDTVKVPYLTANADIK
                     EGDLLVSSGMGGTFPPGYPVAQVIKIVNDPNESFLSITAKPAARLNAGKQVLLIWRGN
                     APKSGGKSK"
     gene            2883..3374
                     /gene="mreD"
                     /locus_tag="AC16FV_05085"
     CDS             2883..3374
                     /gene="mreD"
                     /locus_tag="AC16FV_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR2849469.1"
                     /translation="MRLGPDGRRRATLIGTFAAAVILAIMPFPVWAEPFRPDWVGLVL
                     IYWCLAIPDRVSVGTGWLLGFIQDILYGSLLGSNALGKTLVAFLAVRLHLQLRMFPRW
                     QQAVSVLGLLVANQLLVLWIRGAIGQAPETFSYWTPSIVGALLWPWLFVILRDLRRRG
                     GIA"
     gene            3553..>5340
                     /gene="mrdA"
                     /locus_tag="AC16FV_05090"
     CDS             3553..>5340
                     /gene="mrdA"
                     /locus_tag="AC16FV_05090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR2849470.1"
                     /translation="MLNIPIKDQFRESRVFNARLTVVGAIVLALILALFLRLVYLQIF
                     AHRHFETLSQANRIKPIPIQPPRGLILDRNGVVLAQNYPVYTLEIIPEQVDDMNSLLE
                     ELKQVVSLNESDLKNFQKQLRERPRFENLLLRSHLTEEEAARLAVKRTYFSGVELEAR
                     LQRHYPLAGLGVHFLGYVARINEQELERIDKATYRGMQHIGKLGIEVSYEKPLLGQVG
                     FEKVETNAHGRSLRILERIAPVAGRNLYLNIDAKLQALTEQALGNRRGAVIALEPATG
                     AILAFVSTPTYDPNPFVNGIDPDSYNALLNDPDKPLINRALNGQYSPGSTIKAFLGLA
                     ALERDDFGAGKPITCPGWFTLPGSSHQFRDWKKSGHGTMNLHDAVVQSCDVYFYKLAV
                     AMEIDAMRKFLEPFGFGKKTGVDLLNESEGLLPSPEWRQARGQKWYPGETVITGIGQG
                     SILVTPLQLATAIAAMANHGVRLKPQLVRAMEDAKTRVAQETVPETYAQIPLKDRQHL
                     DIQIQNLTDVVHTNRGTAYGIGYNAPYKIAGKTGTAQVKGIAQGQSYSEKATPERFRD
                     HALFVSFAPVGDPKVAVAVIVENGGHGSSA"
BASE COUNT         1015 a         1694 c         1633 g          998 t
ORIGIN      
        1 accgccgcgg ccgagccgcc cgagctgccg ccggggaccc tgtcggtgcc ccacgggttc
       61 ttcaccgcac cataatagga agtctcgttg gacgacccca tggcgaactc gtccatgttg
      121 gtcttgccga gacacacggc gccggcctgg ttgaatttgt cgataatggt cgcgtcgtag
      181 tgggcgacga agttggacag gattttcgag ccgcaggtgg tgagccagcc gtcggcgcag
      241 aaaatgtcct tctgggcgat ggggacgccg gtcagcgcgc cggcctcgcc cctggcgatg
      301 cgggcatcgg cggccttggc ctgggccagc gaacgctcgg gatccacggt aatgaaggcg
      361 ttgaggctcc gatacttatt aatccggtcc aggaataccc gggtcagctc gacgctcgag
      421 acttccttgc ggcccagggc gcgtgacagc tcggcaacgg ttttttcaaa catgggcttt
      481 cttgacgggt cggcggcgaa aactactcta ttaccttggg gactaggtac aggccggcct
      541 cgaccgccgg cgcgatcttc tggaatttct cgcgctggtc gggctcggtg accgcgtcct
      601 cgcgcagccg caggccggca tcgttcggat gggccatggg cgcgacgccg gcggtatcca
      661 ccgcgttcat ctgctcgatc agaccgagga tgcgggaaag cgtctcggca taggcgtccg
      721 cctcttgccc ggtgatgtgc aaacgcgcaa gatgcgcaat cttgcgtacc tgttcgcggt
      781 ccagagacat gcgatttcgt tcaagggata gaaatgaaat cgaaaactat catattcatc
      841 ccgccggttg aaccgcccga ttgcggttga tacagttagc gcgtttcgaa acgccttcaa
      901 ggacagaatt ctcctcatgt tcaagagtct tcgtggtttc ttttccagtg atatcgccat
      961 cgacctgggc accgccaaca ccctgatctt cgtgcgcgac aagggcatcg tgctgaacga
     1021 accctcggtc gtcgccatcc gccaggatta caccagtcgc aacaaccgcg ccatcctcgc
     1081 cgtcggccac gaagccaaga agatgctcgg ccgcacgccg ggatcgatcc aggcgatacg
     1141 gccgatgaag gaaggcgtca tcgccgactt caccgtcacc ggcgagatgc tgaaatattt
     1201 catccgcaag gtgcacgaga accgtatgtt ccagccgagc ccgcgcatcg tcatctgcgt
     1261 gccctgcggc tcgacgcagg tcgagcgccg cgccatccgc gaggccgcca tgagcaccgg
     1321 cgcgcgcgaa gtatttctta ttgaagaacc gatggccgcc gcgatcggcg ccgacctgcc
     1381 catcatggaa ccgaccggct cgatggtgct cgacatcggc ggcggcacca gcgaggtcgg
     1441 cgtgatctcg ctcggcggca tcgtgtattc gcattcgctg cgcatcgccg gcgacaagat
     1501 ggacgaatcg atcgtcaact acgtgcgccg caaatacggc ttgctcatcg gcgaggccag
     1561 cgccgagcag gtgaagaagg aaatcggcac cgcctgggaa ggcagcgaga tcaagcaggt
     1621 ggaagtcaaa ggccggcaca tcgccgacgg cgtgccgcgc gcgcaggtgc tgaacagcca
     1681 ggaaatccac gccgcgctct cggagtgcct cgccggcatc atcggcgcca tcaagatcgc
     1741 cctcgaacag acgccgccgg aactcggcgc cgacatcgcc gaaaagggac tggtggtctc
     1801 gggcggcggc gcgctgctgc gcgacatcga ccgcatgctg gcggaacaga ccggcctgcc
     1861 gatcatcatc accgaagatc ccctcacctg tgtagtgcgc ggctgcggcc gggcgctcat
     1921 ggaggccgaa acactgggcg atgtatttgt gtacgaatag gttgtcgccg gcattttgtg
     1981 ccggcgtcac ctggatttgc ccgcgcgtcg tcgcggcgcg catgatttat gattaacgtt
     2041 ttcggccgtt ccatcggggc gccgtcccat ctcacccggt ttgtcctgct gtccgccctg
     2101 tcggtggcgc tgatgatgct cgatcaccgc gggcatcatc tggaaaaaat ccgcgccggc
     2161 cttaatactt tggcctaccc catccagatc atcgccgcca ccccggcgta cgtggtcggc
     2221 ggcatggctg acttctttac cacccgcggc acgctgcgcg tggacaacga gaaactgctg
     2281 accgagcgtc aggcgctgct ggccaagctg caacagttcg aggccctgga gcaggaaaac
     2341 cagcgcctgc gccagatgct ggtgagcgcc gcgcaggtgg ccgacaaggc catcgcggcc
     2401 gaactggtcg aggtcagcgc cgaaccgttc acgcgcaagg tcgtcgtcgc caagggtgcc
     2461 caggacggcg tctacgtcgg ccagtcggtg atcgacgcgc acggcatcat ggggcaggtc
     2521 acgcaggtgg ccggcaacgt cagccgcgtg acgctcatca ccgacgccgg ccacgccatt
     2581 cccgtgctcg acaaccgcag cggtctgcgt gccctcgtct tcggcaccgg caatcccgac
     2641 acggtcaagg ttccctacct caccgccaac gccgacatca aggaaggcga cctgctggtg
     2701 agctccggca tgggcgggac gttcccgccg ggttatccgg tggcgcaggt aatcaaaatc
     2761 gtcaacgacc ctaacgaatc gtttctgtcc atcaccgcca aaccggcggc gcggctcaat
     2821 gccgggaaac aggtgctgct gatctggcgc ggcaacgcac ccaagagcgg gggcaaatcc
     2881 aaatgagact ggggccggac gggcgtcgcc gcgcaacgct gatcggcacc ttcgccgccg
     2941 ccgtgattct cgccatcatg ccgtttccgg tatgggccga gccgttccgg cccgactggg
     3001 tcgggctggt gctgatctat tggtgcctcg cgattcccga ccgtgtttcc gtcggcaccg
     3061 gctggctgct cggcttcatt caggacatcc tgtacggctc gctgctcggc agcaacgcgc
     3121 tcggcaaaac gctggtggcg tttctcgccg tgcggctgca tttgcaactg cgcatgttcc
     3181 cgcgctggca acaggcggtg tcggtgctcg gtttgctggt ggccaatcaa ttgctggtgc
     3241 tgtggattcg cggcgccatc ggccaggccc cggagacgtt cagttactgg acgcccagca
     3301 tcgtcggcgc cctgttgtgg ccgtggctgt tcgtcatcct gcgcgacctg cgccggcgcg
     3361 gcggcatcgc ctagatgctt atcctaaaga tctctaaacg cagattaacg cagatttatt
     3421 tttatgatga gcgcagatga tttttagtca gaaacatcga ggtcaggctt ttattccaga
     3481 atttatccgc gttcatctgt gtttttatct gtgttcatct gcggatgcct tgtctttgtt
     3541 tagcgaagct tcatgctgaa tatccccatc aaggatcagt tccgggaaag ccgggttttt
     3601 aatgcacggt tgacggtggt cggcgccatc gtcttggcgc tgatcctggc gctgttcctg
     3661 cgcctggtct atctccagat cttcgcgcac cgccacttcg aaacgctgtc gcaggccaac
     3721 cgcatcaagc cgatcccgat ccagccgccg cgcgggctga ttctcgaccg caatggcgtg
     3781 gtgctggcgc agaactatcc ggtgtacacg ctggaaatca ttcccgagca ggtggacgac
     3841 atgaactcgc tgttggagga attgaagcag gtggtcagtc tcaacgagtc ggacctcaag
     3901 aacttccaga aacagctgcg tgagcgcccg cgcttcgaaa acctgctgct gcgctcgcat
     3961 ctcaccgagg aggaagcggc acggctcgcg gtcaagcgga cctacttcag cggtgtggag
     4021 ctcgaggcac gcttgcagcg gcattatccg ctcgcggggc tgggcgtgca ttttctcggt
     4081 tacgtcgcgc gcatcaacga acaggaactg gagcgcatcg acaaggcgac ctaccgcggc
     4141 atgcaacaca tcggcaagct gggcatcgag gtcagctatg aaaaacccct gctcgggcag
     4201 gtcgggttcg agaaggtcga aaccaacgcc cacgggcgct cgctgcgcat cctcgagcgc
     4261 atcgccccgg tcgcgggccg gaatctttac cttaatatcg atgccaagct tcaagcgctg
     4321 acggaacagg cgctcggcaa ccggcgcggc gcggtcattg ctttggagcc cgcgaccggc
     4381 gccatcctcg ccttcgtcag cacacccact tacgacccca atccgttcgt caacggcatc
     4441 gatccggatt cctacaatgc attgctcaac gacccggata aaccgctgat caaccgcgcg
     4501 ttgaacggcc agtactcgcc cggttccacc atcaaggcct ttctcggact cgccgcgctg
     4561 gaacgggacg acttcggcgc cggcaaaccg atcacctgtc ccggctggtt cacgctgccc
     4621 ggaagctcgc accagttccg cgactggaaa aagagcggac atggaacgat gaacctgcac
     4681 gatgcggtgg tgcaatcctg tgacgtgtat ttctacaagc tcgcggtggc catggaaatc
     4741 gatgccatga gaaaattcct cgagcccttc ggcttcggca agaaaaccgg ggtggacctg
     4801 ttaaacgagt ccgaagggct tctgccttct cccgaatggc gccaggcgcg cggacaaaaa
     4861 tggtaccccg gcgaaaccgt catcaccggc atcggccagg gatcgatcct ggtgacgccg
     4921 ctgcaactgg cgacggccat cgccgcgatg gccaaccacg gcgtgcgcct gaagccgcaa
     4981 ctggtgcgcg ccatggagga cgccaagacc cgggtggcgc aggaaaccgt tcccgagact
     5041 tacgcgcaga ttccgctgaa ggacaggcaa catctcgaca tccagattca gaacctgacg
     5101 gacgtggtgc ataccaaccg gggaaccgcc tacggcatcg gttacaacgc cccctacaag
     5161 atcgccggca aaaccggtac ggcgcaggtg aagggcatcg cccagggcca gtcctacagc
     5221 gaaaaagcca cgccggaacg gtttcgtgac catgcgttgt ttgtttcctt cgcgccggtc
     5281 ggcgatccga aggtggcggt ggccgtgatc gtggaaaacg gcggccacgg cagctccgcc
//
LOCUS       DBNGZE010000196         3222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_306196, whole genome shotgun sequence.
ACCESSION   DBNGZE010000196 DBNGZE010000000
VERSION     DBNGZE010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_306196"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1993
                     /locus_tag="AC16FV_05095"
     CDS             443..1993
                     /locus_tag="AC16FV_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR2849471.1"
                     /translation="MSDRISSWRAVWLALRSPRTAAVSLLSFSSGLPLGLVWIAIPTW
                     LAREGVDIRIIGLFTLAQAPWSFKFLWSPVMDRYALPLPRLGRKLGWTLLWQIALLIG
                     TLLLGGVALQPETLWIIGSLTLAIAFASASQDIAIDAYAVEILEPDEQGVAVGARIAI
                     YRAAMFVAGGLSITLAGLWSWPVMFTILALLYLPMLVVTLLAPKSAADRLQAPPSLRE
                     AVWEPFVGFLSKRRALELLAFVVLYKLADNLGGALIRPFLVQTGFNDFDVGIATATIG
                     LIATLVGTFLGGMLTTAMGLGHALWVFGALQIASNLGYVLIAEIGVNRPLMYGAMGFE
                     SLTTGMGTGAFSVLLLRMTMKQFSATQYALFSSLFALPRILAGPVTGVMVDAIGWREF
                     FLFTIAVGIPGMLMLQRFSPLGRREPEIQQLAEIEPRVLSRAELFRRAVIGGLIGFGI
                     AALSVALLDAFKHQKISATFDVLTPLANLFAPADISGWVSLFGVALFSIVIALATAAT
                     AAARSQKS"
     gene            2011..2277
                     /locus_tag="AC16FV_05100"
     CDS             2011..2277
                     /locus_tag="AC16FV_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR2849472.1"
                     /translation="MARVKIYSTAVCPFCFKAKALLKKWKVPFEEIRIDTDRSKLAEF
                     ARVTNGARTVPQILIDGKCIGGFTELTELHMEGGLDELVEASGQ"
     gene            complement(2314..2643)
                     /locus_tag="AC16FV_05105"
     CDS             complement(2314..2643)
                     /locus_tag="AC16FV_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849473.1"
                     /translation="MMSGLRQLLNWLGPLRLLLVLGVLALLVLRPAPGTPPVSAGWDM
                     LPTLIAPALVPLFFMVLLLDAIMGAVWLSSYPAERRRYQTVIGVSLGMALLLTLWWWP
                     YFGATLR"
     gene            complement(2647..>3222)
                     /locus_tag="AC16FV_05110"
     CDS             complement(2647..>3222)
                     /locus_tag="AC16FV_05110"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR2849474.1"
                     /translation="GVALYARRAPDKVRVGFGSKEHDDEGRYLQADFGKLSIVSIYLP
                     SGSAGPHRQESKFRFMEHFYPVLKKLRHDGREYILCGDWNIAHKEIDLKNWKSNQKNS
                     GFLPEERAWLTQVFDELGWVDVFRTLNDKPDQYTWWSNRGRAYEKNVGWRIDYQIATP
                     GIARKAERERIYKDKRFSDHAPLILDYNHAL"
BASE COUNT          594 a         1002 c          963 g          663 t
ORIGIN      
        1 gtttgtcttg ggacagaggg tgcaagagct atgccacccg ttctaaagct taagaatcaa
       61 caggttgtcc gaaggacggg atcgattcac caagatgaaa ttggactgaa acgggcgctt
      121 taatgacgca tctggtagcg atggcgccgc ccgatattct tttccggcct caattgagat
      181 agcccatggg gcggttttgg tgaacgcctt tcatccgccg ccaaaccgcg aatagcggct
      241 ccggtcttgg tctttgctaa gctggtaaag gcaaaaggca tcatgaagcc gtgtaagatg
      301 ggcgccgctc gccatggacg ggaataatca aattcaggga tccaccattc tccaatctca
      361 aatccaacgg gcgcgccgcg actgcctccc gcttcccgcc tgagtgcgac atgccaaaga
      421 acgcgcgcgg agaaaaaata cagtgagcga ccggatttcc tcatggcgcg ccgtgtggct
      481 ggcgctgcgc agtccgcgca cggccgcggt gagcctgctc tccttttcct cggggctgcc
      541 gctgggtctg gtgtggatcg cgataccgac ctggctcgcg cgcgagggcg tggacatccg
      601 catcatcggt ctgttcacgc tcgcccaagc accgtggagt ttcaaattcc tgtggtcacc
      661 ggtgatggac cgctatgcgc ttcccctgcc acggctcggg cgcaagctcg gctggacgtt
      721 gctgtggcaa atcgcattgt tgatcggcac cttgctgctc ggcggcgtag ccttgcaacc
      781 agagacgctc tggataatcg gcagtcttac gctcgccatc gccttcgcct cggccagcca
      841 ggacatcgcc atcgacgcct acgcggtgga aattctggaa ccggacgaac agggcgtggc
      901 cgtaggcgcg cgcatcgcga tttaccgcgc agccatgttc gtggccggcg ggctcagcat
      961 cacgctggcc ggcctctggt cctggccagt catgttcacg atcctcgccc tgctctacct
     1021 gccgatgctg gtagtcacgc tgctcgcacc caaatccgcc gctgaccgtt tgcaggcgcc
     1081 gccgtcgctg cgcgaagcag tgtgggagcc gttcgtggga tttttatcca agcgccgcgc
     1141 gctcgaattg ctggcgtttg tcgtgcttta caagctcgcc gacaatctcg gcggggcgct
     1201 catccggccg ttcctggtgc agacgggctt caacgatttc gacgtcggca tcgccaccgc
     1261 cacgatcggc ctgatcgcca cgctggtggg caccttcctc ggcggcatgc tgaccacagc
     1321 gatgggcttg ggccacgccc tgtgggtgtt cggcgcgctg cagatcgcat ccaacctcgg
     1381 ctatgtgctg atcgccgaga tcggcgtgaa ccggccgttg atgtacggcg cgatgggttt
     1441 cgagagcctc accaccggca tgggtaccgg cgccttcagt gtgttgttgt tgcgcatgac
     1501 catgaagcag ttttccgcca cccagtacgc gctgttctct agcctgttcg cgttgccgcg
     1561 tatccttgcc ggaccagtga ccggcgtcat ggtggacgcc atcggctggc gtgaattttt
     1621 tctgttcact atcgccgtcg gcatccccgg catgctgatg ctccagcgct tctcgccgct
     1681 gggccggcgt gagccggaga ttcaacaact ggcggagatc gaaccgcggg tgctgagccg
     1741 ggcggaattg ttccggcgcg ctgtcatcgg tggtctcatc ggcttcggca tcgccgcgct
     1801 gtcggtggcg ctgctcgatg ccttcaagca ccagaagatt tcggccacct ttgatgtgct
     1861 tacgcctctg gccaatttgt tcgcgccggc ggatatttcg ggctgggtca gcttgttcgg
     1921 cgtggcctta ttcagtatcg tcattgcgct cgccaccgcc gcgaccgcgg cggcgcgatc
     1981 acaaaaatcc tgaagataaa acggatacca atggcaagag tcaaaattta cagcaccgcc
     2041 gtttgcccct tctgcttcaa ggccaaggcg ctgctcaaga aatggaaggt cccgttcgag
     2101 gaaatccgca tcgacaccga tcgcagcaag ctcgccgagt tcgcgcgcgt caccaacggc
     2161 gcgcgcacgg ttccccagat cctgatcgac ggcaaatgca tcggcggctt caccgagctg
     2221 accgaactgc acatggaagg cgggctggat gagctcgtgg aagcatccgg tcaatagccg
     2281 cgtttccgcc cgaccaccct ttcttcgctg agatcagcgt aacgtggcgc cgaaataggg
     2341 ccaccaccac agcgtcagta acagcgccat tccgaggctc acgccgatga cggtttgata
     2401 gcgtcgccgc tccgcggggt aactcgagag ccacaccgca cccatgatgg catcgagcag
     2461 cagaaccatg aagaaaagcg gcacgagtgc cggagcgatc agcgtgggca acatgtccca
     2521 gccggcggag accggcggag tacccggtgc cgggcgcagc accagcaacg ccagcacgcc
     2581 gagcacgagc aacaatcgca gcggtcccag ccagttcaac agttgtcgca gtccggacat
     2641 catgattcag agcgcgtggt tgtaatccag aattaacggc gcgtggtcgg agaagcgctt
     2701 gtccttatat atgcgctctc tctccgcctt cctggcgatg cccggcgtgg cgatctgata
     2761 gtcgatacgc caacccacgt tcttttcata ggcccgaccg cggttggacc accaggtata
     2821 ttgatcgggc ttgtcgttta gcgtgcggaa cacgtccacc cagccaagct cgtcgaacac
     2881 ttgcgttagc caggcgcgct cctcgggcag gaaaccggaa ttcttctggt tcgatttcca
     2941 gtttttcagg tcgatctcct tgtgcgcgat attccagtcg ccgcagagaa tgtattcgcg
     3001 cccgtcgtgg cgcagtttct tcagtaccgg gtagaagtgt tccatgaaac ggaacttgga
     3061 ttcctgccgg tgcgggccgg ccgagcccga aggcaggtag attgaaacga tgctgagctt
     3121 gccgaaatcg gcctgaagat agcgcccttc atcatcgtgc tccttcgagc cgaatccgac
     3181 acgcaccttg tccggcgcgc ggcgcgcata cagcgccacg cc
//
LOCUS       DBNGZE010000197         3975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_445191, whole genome shotgun sequence.
ACCESSION   DBNGZE010000197 DBNGZE010000000
VERSION     DBNGZE010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_445191"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="egtB"
                     /locus_tag="AC16FV_05115"
     CDS             complement(<1..1136)
                     /gene="egtB"
                     /locus_tag="AC16FV_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR2849475.1"
                     /translation="MSFVRGKPPQAERTARSLPTARETLAARYSGVRRMSEALCQPLA
                     PDDYGLQAMPDVSPAKWHLAHTSWFFETFLLKSFRPGYHPFHPQFEQLFNSYYEQLGA
                     PFPRPRRGLLSRPTTEEIFRYRAQVDDAMAALFASVEETHWPEVAARATLGCHHEEQH
                     QELLLTDIKFNFSVNPLKPAYRADLPVAPAGRNVTLEWIEQPGGIQEIGHDGTGFAFD
                     NESPRHRVLLPPYALGSRPVANGEYLAFVETGGYQRPEYWLADGWRVAHEQAWQAPLY
                     WEKIDGRWWLFTLAGMRALNVDEPVCHVSFYEADAYARWAGKRLPSEAEWETIARREP
                     VRGNLREAGYLHPVPAPADGIPAQIFGDVWEWTRSPYASYPGYRP"
     gene            1244..1528
                     /locus_tag="AC16FV_05120"
     CDS             1244..1528
                     /locus_tag="AC16FV_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR2849476.1"
                     /translation="MTQAIWNGAVLAESDACHIIEGNYYFPSESVRREFLRPSKMHTT
                     CRWKGVASYYDVVVNDKVNCKAAWHYPQPKDAALQIQHYVAFWHGVKIVP"
     gene            1864..2412
                     /locus_tag="AC16FV_05125"
     CDS             1864..2412
                     /locus_tag="AC16FV_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR2849477.1"
                     /translation="MWKIIGGFFGGIVFTGLVAWQMMPSMMFNERVSPYDMEETIARI
                     QQNIQQAGNGWTLTGLRNPAKAIQNDGGNVLPVMMVETCSPKYSKPILKEDNVRFLSI
                     LMPCKISVYKKSDGKTYIGSMNAGLMGKMFGPVVGEVMGHVAQDQVKFLTFDPSKPAP
                     KMIEGVPGGGSGGSGGGGGGAC"
     gene            2473..>3975
                     /locus_tag="AC16FV_05130"
     CDS             2473..>3975
                     /locus_tag="AC16FV_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HIR2849478.1"
                     /translation="MLKPRHVSVPMLILGTMCAAISAWADIKPSAPAATPPAAAVVGA
                     PAVEEKKPSSSTADHSKFKELQKTFATGPDVTKACLTCHTEAAKQIHKTKHWTWEFLN
                     PESKQRLGKKNIVNNFCTAVPSNYTFCTACHVGYGWKDQNFDFTSEVNVDCLVCHDTT
                     GTYRKLAGFSGHPPYKDIEFPPHSGKIVKAVNLKDIAQHVGKTSRATCGACHFYGGGG
                     DGVKHGDLDSSMKNPGKYLDVHMDKNGLNFTCATCHQTSGHDIPGSRYTPTAMDVKGA
                     RIRGKIDAANPVSCESCHGSKPHPAKMAKLNDHTDKLACQVCHIPLFARGGVPTKMLW
                     DWSTAGKLDKDGKPFLLKDSGGHVSYDSKKGDFKSESHVIPEYIWFNGTVKYTLLGDK
                     LDPAKVTQINRFEGSPTDGKSKIWPVKVFHGKQPYDKATLSLLVPHTFGPDDSAFWSN
                     FDWKKSLEYGMREVNREFSGQYGFVSTEMSWPITHMVAPKDDALECRQCHS"
BASE COUNT          865 a         1179 c         1190 g          741 t
ORIGIN      
        1 ggacggtagc cgggatagga tgcgtagggg ctgcgggtcc attcccacac gtcgccgaat
       61 atttgcgccg gaattccatc ggcgggagcg ggaaccggat gcaggtatcc cgcctcgcgc
      121 agattgccgc gcaccggttc gcgtctggcg atcgtctccc attcggcctc gctcggcaga
      181 cgcttgccgg cccagcgcgc gtaggcgtcg gcctcgtaga agctcacatg acataccggc
      241 tcatctacgt tcagcgcgcg cattccggcg agcgtgaaga gccaccagcg gccgtcgatt
      301 ttttcccagt acaacggtgc ctgccacgcc tgttcgtgcg cgacgcgcca accatccgcg
      361 agccagtatt ccgggcgctg gtaaccaccg gtctcgacga acgcgagata ctccccattc
      421 gcgaccggac gcgagcccag cgcatagggt gggagcaaca cacggtgacg cggggattcg
      481 ttgtcgaaag caaagccggt gccgtcatgt ccgatttcct gtatgccgcc cggctgctcg
      541 atccattcca gcgtcacgtt cctgccggcg ggtgcgaccg gcaggtcggc gcggtaggcc
      601 ggcttgagcg gattgaccga gaagttgaac ttgatatctg tcagcaacaa ctcttggtgc
      661 tgttcctcgt ggtggcaacc gagcgtggcg cgcgccgcca cttccggcca gtgcgtttct
      721 tccacggagg cgaacaacgc tgccatcgca tcgtctacct gtgcacgata gcgaaaaatt
      781 tcctcggtcg tggggcgcga cagcaggccg cgtcgcggac gtgggaacgg cgcgccgagc
      841 tgttcataat aagaattgaa cagctgttcg aactgcgggt gaaacggatg gtaaccggga
      901 cgaaagcttt tgagcaggaa ggtttcgaag aaccaggacg tgtgcgcgag gtgccacttg
      961 gccggactga catcgggcat ggcctgcaag ccataatcgt ccggcgcgag cggttggcac
     1021 agcgcctcgc tcatgcgccg cacgccgctg tatcgtgccg ccaacgtctc ccgcgcagtg
     1081 ggaaggctac gcgcggtgcg ttctgcttgt ggtggcttgc cgcggacgaa gctcatacta
     1141 agcctccctg cgatacgagg gtcaattgaa acacagccgc cgcggatttc aaaccccgtg
     1201 cggtggtcaa aggcgataac tccacattct gtgagggttg atgatgactc aggccatctg
     1261 gaatggtgcg gtgctggctg aaagtgatgc gtgccacatc atcgaaggca attattattt
     1321 cccgtcggaa tcggtgcggc gcgaatttct gcgcccaagc aagatgcaca ctacttgccg
     1381 ctggaaaggc gttgcgagtt attacgatgt cgtggtcaac gacaaagtaa actgcaaggc
     1441 cgcatggcat tacccgcagc ccaaagacgc cgccctccag attcagcatt acgtcgcctt
     1501 ctggcatggc gtgaaaattg tgccgtgagt ttcttgctgt gggtaaccgc taaatccctg
     1561 aaaaaatctc cctacctgtt cctgtgtctt agcaaatgca tctgtcgcgt aaattgatcg
     1621 ttttcgtgtt ctgccaacac gccaccgcga aatcttgagc gggatggtca ggattattga
     1681 cctttgtcat tactaaaaaa ataatggaat gacatagtaa aaacgcatat atcggtacat
     1741 gctaatttgc tgatatacca aaattgtcac aggcggacga ttggcaaatg acgaaacgat
     1801 aggcacgcgg cagtggcagg cggtgccgac gcgtgagcga ctttaacgag cggagatacg
     1861 accatgtgga aaatcatcgg aggttttttt ggcggcatcg tgttcactgg gctcgtcgcc
     1921 tggcaaatga tgcccagcat gatgttcaac gagcgggtca gcccttatga catggaagag
     1981 accatcgcgc gcatccagca gaacatccag caggccggca acggctggac cctcaccggt
     2041 ttgcgcaatc cagcgaaggc catacagaac gacggcggca acgtgctgcc ggtgatgatg
     2101 gtcgagacct gcagccccaa gtactcgaag ccgatcctga aggaagacaa cgtgcgcttc
     2161 ctctcgatcc tgatgccgtg caagatttcg gtgtacaaga agagcgacgg caagacctac
     2221 atcggcagca tgaacgcagg actgatgggc aagatgttcg gcccggtggt gggcgaggtg
     2281 atgggacatg tcgcgcagga ccaggtcaag tttctcacct tcgatccgag caagccggcg
     2341 cccaagatga tcgagggcgt tccgggcggc ggctcgggag gcagcggcgg cgggggtggc
     2401 ggtgcatgct gagcgccgtc attgcgtcac ggaacattac gctcgcgatt tctgaaaagt
     2461 aaggagacgc ccatgctgaa acctcgacat gtatctgttc caatgctgat tctgggaacg
     2521 atgtgtgcgg ccatctctgc ctgggccgac atcaaaccgt ccgcgccggc ggcgactcca
     2581 ccagcggctg cggtggtggg cgcgccggcg gtggaagaga agaaaccttc ctcttccacc
     2641 gccgatcatt ccaagttcaa ggagctgcag aaaaccttcg ccaccggtcc ggacgtcacc
     2701 aaggcctgtc tgacctgcca caccgaagcg gcgaagcaga ttcacaagac caaacactgg
     2761 acctgggagt ttctgaatcc ggaaagtaaa cagcggctcg gcaagaagaa catcgtcaat
     2821 aacttctgca ctgcggtgcc ttccaattac acgttctgca ccgcctgcca cgtcggctac
     2881 ggctggaagg atcagaactt cgacttcact tccgaggtca acgtggattg cctcgtgtgc
     2941 cacgacacca ccggcactta ccgcaagctc gcgggattct ccggtcatcc gccgtacaag
     3001 gacatcgagt tcccgccgca ctcgggcaag atcgtcaagg cggtaaacct caaggacatc
     3061 gcgcaacacg tgggcaagac cagccgcgcc acttgcggcg cctgtcactt ctacggcggc
     3121 ggcggcgacg gcgtgaagca cggcgatctc gacagctcga tgaagaatcc gggcaagtat
     3181 ctcgacgtgc acatggacaa gaacgggctc aacttcacct gcgccacctg ccatcagacg
     3241 tcggggcacg atatccccgg cagtcgctac acgccgaccg cgatggacgt caagggcgcc
     3301 cgcatccgcg gcaagatcga cgccgccaat ccggtgtcgt gcgaatcctg ccacggcagc
     3361 aagccgcatc cggccaaaat ggcgaagctg aacgaccaca ccgacaagct cgcctgccag
     3421 gtctgccaca ttccgctgtt cgcgcgcggc ggcgttccga ccaagatgct gtgggactgg
     3481 tccaccgccg gcaagctcga caaggacggc aagccgttct tgctcaagga tagcggcggc
     3541 catgtctcct atgacagcaa gaagggcgac ttcaagtccg agtcacacgt gattccggag
     3601 tacatctggt tcaacggcac ggtaaagtac acgctgctcg gcgacaagct cgatccggcc
     3661 aaggtcacgc agatcaaccg cttcgaaggc agtccgaccg acggcaaatc caagatctgg
     3721 ccggtgaagg tgttccacgg caagcagccg tacgacaagg cgacgttgtc actgctggtc
     3781 ccacacacct tcggccccga cgacagcgcc ttctggtcca atttcgattg gaagaagtcg
     3841 cttgagtacg gcatgcgcga ggtgaaccgc gagttcagcg gccagtacgg tttcgtctcg
     3901 accgagatga gctggccgat cacgcacatg gtcgcgccca aggacgacgc gttggaatgc
     3961 cgtcagtgcc acagc
//
LOCUS       DBNGZE010000198         9083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1904251, whole genome shotgun sequence.
ACCESSION   DBNGZE010000198 DBNGZE010000000
VERSION     DBNGZE010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1904251"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="AC16FV_05135"
     CDS             <1..2235
                     /locus_tag="AC16FV_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2849479.1"
                     /translation="LERIRPTLIPEGVQVNVTRNDGAKANDAVNLLMEHLAIAIASVV
                     ALLVFFLGWRAASIVTITIPLILFITLAIDFAAGQTINRITLFALILSLGLLVDDSIV
                     VIENIFRHYAQKGADRLKGAIAAVNEIGKPTNLATFTVMFAFLPMFWVTGMMGPYMAP
                     IPFNVPIAMLTSLAIAYTVAPWAAYRFLKIQGHSHEGAEHHGHHGQLERLYERHMGRL
                     IGSRGARNGFFIGVGLLLVVVLLLPAFGWVKFQMLPKNNTNTFNITVDMPEGTSLEET
                     DRVARLVGDIVRRHPQVETYEATVGEAGVVDFNGLLRGANLKTGSNVADVRVNLRNKH
                     ERGESSIDIVLELRQPIAELARQTGANIKLVEDPPGPPVRATILAELYGPDYAQLRKI
                     ARELRREVFEQTEDVVDIDDSVTDDWTEYRVVVDRGRATLAGIPPAQVAETLHAFLAG
                     LDASTVHLELEKEPVPIRFRIPAADRAGPQDLSKIFFTSPQGRQVPLSDIARVESRGA
                     PKPIYYKDQYPVVYVSGELGSSSQVYAVLKMWNWLRNHELPSGTKLTQYFMADPDTSG
                     YAVRWDGEMRLTLDVFRDLGSAFAVAIILIYLILVGYYRSFAIPLIVMGAIPLTVVGV
                     LPGHMIMGQHFTATSMIGVIALAGIVVRNSLLLIDFILEYRKGGHTLDEAVLQAGVVR
                     LRPILLTAFAIILGTLIMAFDPVFGGLAISLIYGTFASTVLTLFVIPLVYFIYEKRRA
                     IPTA"
     gene            complement(2245..2688)
                     /locus_tag="AC16FV_05140"
     CDS             complement(2245..2688)
                     /locus_tag="AC16FV_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849480.1"
                     /translation="MSAQMNEDPFYAAFRGRFTGVLSWEDLDAFWEVVRNNAAAGWYV
                     YAIGEAPPAAPATAEQVNTFLAEVDVLLRREHEEDYCGIVYTDSKTEPSMIKIFDPNN
                     LGVQCGFSDNPPLPGWVLSRLPPVALHDRAHRPENRKRWWRKLWS"
     gene            complement(2704..3336)
                     /gene="dsrE2"
                     /locus_tag="AC16FV_05145"
     CDS             complement(2704..3336)
                     /gene="dsrE2"
                     /locus_tag="AC16FV_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009562368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein DsrE2"
                     /protein_id="HIR2849481.1"
                     /translation="MSNVAEVSPGKFSSELLQKHGAVLDAWFDERFAKKLKETEESKT
                     PSLSIIATKGTMDWAYPPFILASTASALGWDVNVFFTFYGLELLKKDLNLQVSPLGNP
                     GMPMKMPFGPKWFQGVNWNIPNLVMAGVPGFEKLATTLMKKTVKNNGVASIQELRDVC
                     IEAGVNLVACQMTVDLFGYTRDDFIPEVKDWIGAASFLPIAQKSDVCLFI"
     gene            3556..3957
                     /locus_tag="AC16FV_05150"
     CDS             3556..3957
                     /locus_tag="AC16FV_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR2849482.1"
                     /translation="MENLDVVKLLKAAQNLERNISVSLMYSGLRLPQYRLLDALAGME
                     QATVTEMSEKLSIRRATASVLINELIESGIVEVTENNSDRRSFHIRLTRMGAGKLEAA
                     RKDIAVFREKLSQKYSPETIKMLNEFADRLA"
     gene            complement(4051..4281)
                     /locus_tag="AC16FV_05155"
     CDS             complement(4051..4281)
                     /locus_tag="AC16FV_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR2849483.1"
                     /translation="MSNTTQELDARGLNCPLPILRAKKALNGLQQGQVLRIRATDPGA
                     VKDFDAFCKSTGNELVSSEQNSGEYVFEIRKH"
     gene            complement(4348..4911)
                     /locus_tag="AC16FV_05160"
     CDS             complement(4348..4911)
                     /locus_tag="AC16FV_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR2849484.1"
                     /translation="MENKGSDKRVRLIEAANKLIHQQGFNQTTLADIARESEVPLGNV
                     YYYFKTKEEIGSALIEHRADFYRGLIESWNKLPHPKQRLVALIKMVSDERETLAQSGC
                     PIGSLCQELHKDGGPLADKSASLMAAILSWTEQQFRLLSKEKESSDLALHLVSALQGA
                     SLLTHTFRQPELIARETNRLRDWVNAL"
     gene            5094..6167
                     /locus_tag="AC16FV_05165"
     CDS             5094..6167
                     /locus_tag="AC16FV_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR2849485.1"
                     /translation="MDTLTHALSGALLARATEPQNPRTDQLPRRARMWVGFWAAAFPD
                     SDFITNFIDPLTYLTVHRGITHSVIMLPLWALGLSLLFMLLVRGKYSWRAFVGVVALG
                     IGVHIVGDVITAFGTMIFAPLSIWRAQIPTTFIIDPYFTAILVAGLVASAVWNLTRRP
                     AIIGLAVLAAYVGFQGILHQRAVAAGQDYATRHGLVAGTVEAIPQPFSPFNWMIVIPQ
                     KDEYYMAYVSLWRSEIPEFPPEGANWLRQVYASYYPLRDAMWKQVPRFGLGKDAKLAE
                     ELWYSDVLSPYRNFALFPALYGVDRMQGRLCVWFNDLRFSLAGRNMPFRYGACQTGKD
                     AAWHVYRLLNDDNGQEIFEAIPH"
     gene            complement(6335..8533)
                     /locus_tag="AC16FV_05170"
     CDS             complement(6335..8533)
                     /locus_tag="AC16FV_05170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR2849486.1"
                     /translation="MSATSRIYQVAVPTPLYRAFDYLAPVMLAPGVRVRVPFGRREVV
                     GVVVGEVARSELPSNRLKSITQPLDTRAIMPSSMLSLLQWAADYYHHPVGEVIHTALP
                     VRLRQGHAPVVGGVKVWSLTPAGRAADPQTLKRAPAQKHVFEALAAATEGLDAEQLAQ
                     VSQRFAAALKALRAKGWVSSHQRERQPVVNGNLQAAPSLNPDQKSAVTAMLAGLGGFH
                     PFLLHGVTGSGKTEVYLSVIEQALAQGKQALVLVPEISLTPQLVGRFERRFHTPIAVL
                     HSGLNEHERLSAWLRATEGKAPIVLGTRSAVFTPLKNPGLIVVDEEHDASYKQQDGFR
                     YSARDVAVMRAAREKIPIILGSATPSLESLKNARQNAYALIELPERTGSAVMPEVRLL
                     DMRRLKPSEGLSLPLREALADRLKKGEQSILFLNRRGFSPVWMCFDCGWVAPCKRCDA
                     QLTLHHRKQKLLCHHCGAEQEVMRRCPSCQSDNLHPLGEGTERVEGALQKFFPQSRIE
                     RIDRDSTARRGALEEKLRRVHEGEADILVGTQMLSKGHDFPNVTLVGILNADQGLYGT
                     DFRSGERLFQLIMQVSGRAGRADKPGEVLIQTWHPDHPLFAALQRHDFHGFAEFALNE
                     RRETGYPPYSHLALLRAESPAPGAALEFLREARALAVQLSPGKDVQLLSPAPAPMERR
                     AGRYRAQLLVQSAQRAPLHEFLAQWVRQLTEAKFAKKTRWSLDVDPVDMY"
     gene            complement(8859..>9083)
                     /locus_tag="AC16FV_05175"
     CDS             complement(8859..>9083)
                     /locus_tag="AC16FV_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849487.1"
                     /translation="ETINRFDSSSRPISGPYCFTHLHAPFMFILLFRGSRHRIPCFSR
                     LPAQGCSKNSQISPDDSVGGCLFYAQRNRI"
BASE COUNT         1847 a         2698 c         2618 g         1920 t
ORIGIN      
        1 ctggaacgta tccgcccgac gctgattccc gagggcgtgc aagtgaatgt cacgcgcaac
       61 gacggcgcca aggctaacga cgccgtcaac ctgctgatgg aacatctcgc catcgccatc
      121 gccagtgtgg tcgcgttgct ggtgttcttc ctcggctggc gggcagcctc cattgtcacc
      181 atcaccattc cactgattct ttttatcacg ctcgccatcg atttcgccgc aggccagacc
      241 attaatcgca ttacgctgtt cgcactcatt ctctcacttg gcctgctggt cgacgattcg
      301 atcgtggtca tcgaaaacat ttttcggcac tatgcacaga aaggtgcgga ccggctcaag
      361 ggcgcgattg cggcggtgaa cgagatcggt aagccgacca atctcgccac cttcacggtg
      421 atgttcgcgt tcctgccgat gttctgggtc accggcatga tgggaccgta catggcgccg
      481 atcccgttca atgtgccgat tgcgatgctc acctcgctcg ccatcgccta caccgtggcg
      541 ccgtgggccg cgtaccgttt tctcaagata caaggacact cccacgaggg cgcggaacat
      601 catggacatc acgggcagct tgagcgtctg tatgaacgtc acatgggacg gctcatcggt
      661 tcacgcgggg cgcgcaacgg tttcttcatc ggcgtgggtc tcctgctcgt cgtggtgttg
      721 ttgctaccgg ccttcggctg ggtgaaattc cagatgctgc ccaagaacaa caccaacact
      781 ttcaacatca ccgtcgacat gcccgaaggc accagcctcg aagaaaccga ccgtgtggcg
      841 cggctcgtcg gcgacatcgt gcgacgtcac ccgcaggttg agacatatga ggccacggtc
      901 ggcgaggccg gcgtggtcga cttcaatggg ttgctgcgcg gtgccaacct gaaaaccggc
      961 tcgaacgtgg ctgatgtgcg tgtaaacctg cgcaacaagc acgaacgcgg cgaatcctcg
     1021 atcgacatcg tgctcgaatt gcgccagccg atcgcggaac tggcgcggca gaccggcgcc
     1081 aatatcaaac tggtcgagga cccgccgggt ccgccggtgc gcgccacgat tctggccgaa
     1141 ctctatggac ccgattacgc acagctgcgc aagatagcca gggaattgcg gcgcgaggtg
     1201 ttcgagcaga ccgaagatgt ggtcgacatc gatgattcgg tgaccgacga ctggactgaa
     1261 tatcgcgtcg tggtggatcg cgggcgtgcc acgctcgccg gcatcccgcc ggcgcaggtg
     1321 gcggaaacgc tgcacgcctt ccttgccggg ctcgacgcca gcaccgtaca tctggagctc
     1381 gagaaggaac cggtgccgat ccgcttccgc attcccgccg ccgaccgcgc cgggccgcag
     1441 gatttgtcaa aaatcttctt caccagtccg cagggccggc aggtgccgct cagcgacatc
     1501 gcgcgcgtcg aaagccgcgg cgcgcccaaa cccatttact acaaggatca gtacccggtg
     1561 gtgtacgtga gcggcgaact cggttcgagc tcgcaggtat acgcggtgct caagatgtgg
     1621 aactggttgc gcaaccacga gttgccgtcg gggacaaaac tgacacagta ttttatggcc
     1681 gacccggaca ccagcggtta cgcggtgcgc tgggacggcg agatgcgcct gacgctggac
     1741 gtgttccgcg acctgggttc ggccttcgcg gtggcgatta ttctgatcta cctgattctc
     1801 gtcggttatt accgctcgtt tgcgatcccg ctcatcgtca tgggcgcgat cccgctcacc
     1861 gtcgtcggcg tgctgccggg gcacatgatc atgggccagc acttcaccgc tacctcaatg
     1921 atcggcgtga tcgcactcgc cggcatcgtg gtgcgcaact cgctgctgct gattgatttc
     1981 atcctcgagt accgcaaggg cgggcatacg ctcgacgagg cggtgttgca ggccggcgtg
     2041 gtgcgcctgc gcccgatcct gctcaccgcc ttcgccatca tcctgggcac gctgatcatg
     2101 gccttcgacc ccgtgttcgg cgggctggcg atctcgctca tctatggcac cttcgcctcg
     2161 acagtgctga cgctattcgt aatccccttg gtgtatttca tctacgaaaa acgccgcgcc
     2221 attcccactg cgtaatccgc tgatctacga ccagagtttg cgccaccagc gcttgcggtt
     2281 ttccgggcgg tgcgcacggt catgcagcgc caccggcggc aaccggctca ggacccagcc
     2341 cggcaacggc gggttgtcgc tgaaaccgca ttgcacgccg aggttgttcg gatcgaaaat
     2401 cttgatcatc gatggctcgg tcttgctgtc ggtgtatacg atgccgcagt aatcctcctc
     2461 atgctcgcgg cgcagcagga cgtcgacttc ggcgagaaag gtattgacct gctcggccgt
     2521 ggcaggcgcg gctggaggtg cttcaccgat ggcatacaca taccaaccgg cggcggcatt
     2581 gttgcgcacc acctcccaga aggcgtcgag atcctcccag ctcaggacgc cggtgaagcg
     2641 gccgcgaaac gcagcataga acggatcttc attcatctgc gcactcatgg ctttcctttt
     2701 ttatcagata aacagacaaa cgtccgattt ctgcgcgatc ggcaggaagc tggcggcgcc
     2761 gatccagtcc ttgacctcgg gaatgaaatc atcccgtgtg tagccgaaca gatcaaccgt
     2821 catctggcac gcgaccagat tgacgccggc ctcgatacag acgtcgcgca attcctggat
     2881 agacgcgacg ccgttgttct tcacggtctt cttcatcagc gtggtcgcca gcttctcgaa
     2941 accgggcacg ccggccatga ccaggttcgg gatgttccaa ttgacgccct ggaaccactt
     3001 cggcccgaac ggcattttca tgggcatgcc cggattcccg agcggactca cctggagatt
     3061 gaggtctttt ttcagcagct ccagaccata gaaagtgaaa aacacattca cgtcccagcc
     3121 cagcgccgag gccgtggacg ccagaatgaa cggcggatag gcccagtcca tggtgccctt
     3181 ggtggcgatg atggagagcg acggtgtttt gctttcctcg gtctccttca gttttttcgc
     3241 gaagcgctcg tcgaaccagg catcgagcac cgcaccgtgc ttctgcaaca gttcactgga
     3301 aaatttacca ggggatactt cggctacgtt ggacatgagg cctccttgag atttcacgaa
     3361 atgttctgat gaatgtcacc gcaacttccg cccggattgt ttatatgctt cggcggccga
     3421 cgcggattga tatgaagatt ggcatcacct atagtcaatc ggcatattct gaaatgatta
     3481 aatatgccgg acgggcgaag cacaaatcag agaatttaat tagtttcgct aacaaacaag
     3541 actgggggcc acggcatgga aaatctcgat gtcgtcaaac tgctcaaggc ggcgcagaac
     3601 ctggagcgca acatctcggt gtcgctgatg tactcggggc tgcgccttcc ccagtaccgg
     3661 ctgctcgatg cactggcggg aatggagcag gcgacggtga cggaaatgag cgagaagttg
     3721 agcatccggc gcgccacggc cagcgtgctg atcaatgaac tgatcgaatc ggggatcgtt
     3781 gaggtgaccg aaaacaactc ggaccggcgt tcgttccaca tccgccttac ccgcatgggc
     3841 gcgggcaagc tcgaagcggc gcgcaaggac atcgccgtgt tccgcgaaaa actgtctcaa
     3901 aaatattcgc cggaaacgat aaagatgttg aatgaattcg ccgaccggct ggcctagccg
     3961 atcggcgaat tgggccgagc acgcatgtgt gatcgtcaag cgcggttact ccttagtaaa
     4021 cgcttgtcat gaccgacgga ggtttatccg tcagtgcttg cgaatctcga acacgtactc
     4081 cccgctgttc tgttcggaag aaacgagttc attgccagtc gacttgcaga aggcatcgaa
     4141 atccttgacc gcgcccggat cggtggcgcg gatgcgcaga acctgaccct gctgcaatcc
     4201 gttgagggcc ttcttggcgc gcaaaatggg tagcgggcaa ttcaatccac gggcgtcgag
     4261 ttcctgggtt gtgtttgaca tggcgttctc ctgtttgggt tgagggggtc ttcatatttc
     4321 agttgggcac tcgaaccggt tgatgcgcta cagggcgttg acccagtccc tgagccggtt
     4381 tgtttcgcgc gcgatcaatt ccggttggcg gaaggtgtgg gttaacaaac tggcgccctg
     4441 cagcgccgag acaaggtgca atgcgagatc ggaagattct ttctctttgc tcagcaggcg
     4501 aaactgctgc tcagtccagg ataatatggc ggccatcagg ctggcggact tgtccgccag
     4561 cgggccgcca tccttgtgca gctcctggca caggctgccg atggggcagc cgctttgggc
     4621 gagggtttcg cgctcgtcgg acaccatctt gatcagcgcc accagtctct gcttcgggtg
     4681 gggcagtttg ttccatgatt cgatcaagcc acgataaaaa tccgcgcggt gttcaatcag
     4741 ggcactgccg atttcctcct tggtcttgaa gtagtaatac acattgccta acggcacctc
     4801 ggactcccgg gcgatgtcgg cgagcgtggt ctggttgaag ccttgctgat gaatcagctt
     4861 gttggccgct tcaatcaggc gcacacgctt gtctgatcct ttattttcca tttaaattca
     4921 tctacttatt atataagtta gttatctggc taaattatgg attactaata tagacctgtc
     4981 aagcaagaaa ccatgcaatc tctgatgggg atgctcctga cacaaagttg agcatcaaag
     5041 ccctgcctga tgccacgata tactttcttg aattgccgct gaattgaaaa aacatggaca
     5101 ccctgacaca tgccttgagc ggcgcgctgc tggcgcgcgc gacggaaccg caaaacccgc
     5161 gaaccgatca gttgccgcgg cgcgcacgca tgtgggtcgg cttctgggcc gcggcgtttc
     5221 ccgacagcga tttcatcaca aacttcatcg atccccttac ctacctcacc gtgcatcgcg
     5281 gcatcacgca ctcggtgatc atgctgccgc tgtgggcgct gggcctgtcg ctgttgttca
     5341 tgctgctcgt gcgcggaaaa tattcctggc gtgcctttgt gggcgtggtg gcgctgggca
     5401 tcggcgttca tatcgtcggc gacgtcatca ccgccttcgg caccatgatc ttcgcgccgc
     5461 tctccatctg gcgcgcgcag ataccgacga cctttatcat cgatccctat ttcacggcga
     5521 tactcgttgc cggactcgtg gcctcggcag tgtggaatct cacacgcagg cccgccatca
     5581 tcggcttggc cgtgctcgcc gcctacgtgg gctttcaggg aattctgcat cagcgcgccg
     5641 tcgcggcagg ccaggactat gcgacccggc atggactcgt ggccggcacg gtcgaggcta
     5701 ttccccagcc gttttcgccg ttcaattgga tgattgtcat cccgcaaaag gatgagtact
     5761 acatggccta cgtgagtctc tggcgcagcg agatcccgga atttccccca gagggcgcca
     5821 attggctgcg gcaggtttat gcctcgtatt acccattgcg ggatgctatg tggaaacagg
     5881 taccgcgttt cggtttgggc aaggatgcca aactcgcgga agagctgtgg tattccgatg
     5941 tgctttcgcc ctatcggaat tttgccctgt tcccggcgct atatggcgtg gatcgcatgc
     6001 agggcagact atgtgtgtgg ttcaacgatc tgcgtttttc cctggcgggc aggaatatgc
     6061 ccttccgtta tggcgcgtgt cagactggca aggatgcggc atggcatgtt taccgcctgc
     6121 tcaacgacga caacggccag gaaatcttcg aagccattcc acactagcat taaataattt
     6181 ctcgccgcaa agacgcgaag aacgcaaaga aattctccat caaaactagc cagaaaaaat
     6241 ctttgcgccc attgtgctgt acaggaagta cgaatgtcgc gggcgcaggg atgcgcggga
     6301 gcgaccgcct ttgcagcaaa taatcatatc ttccctaata catatccacg ggatcgacat
     6361 ccagcgacca gcgcgttttc ttggcgaact tcgcctccgt caactgccgc acccactgag
     6421 cgagaaattc atgcagcggc gcgcgttggg cggattgcac caacaattgc gcgcggtaac
     6481 gtccggcgcg ccgttccatg ggcgccggtg ccggtgacag cagctgaacg tccttcccgg
     6541 gtgacaactg caccgcaagc gcgcgtgcct cacgcagaaa ttcgagcgcc gcgcccggcg
     6601 cgggcgactc ggcacgcaac agcgccagat ggctgtaggg cggatagccg gtctcgcggc
     6661 gctcattcag cgcgaattcg gcgaagccgt gaaagtcatg ccgctgcaac gcggcgaaca
     6721 agggatgatc cggatgccag gtttgaatca acacctcgcc cggcttgtcg gcgcggccgg
     6781 cgcggccgct cacctgcatg atcaactgga acagccgttc gcccgagcgg aaatcggtcc
     6841 cataaagccc ctgatcggcg ttcagaatcc cgaccagcgt gacattgggg aaatcatggc
     6901 ctttggagag catctgtgtc ccgaccagga tatcagcctc accctcgtga acgcgccgca
     6961 gtttttcctc gagcgcgccg cggcgcgcgg tgctgtcacg gtcgatgcgt tcgatgcgcg
     7021 actgcgggaa aaatttttgc aatgcgcctt ccacccgttc cgtgccctcg ccgagtggat
     7081 gcaggttatc gctctggcag gacgggcaac gccgcatgac ttcctgttcc gcgccacaat
     7141 gatgacaaag cagtttctgc ttcctgtggt gcagggtgag ctgcgcgtca catcgcttgc
     7201 agggcgccac ccagccgcag tcaaaacaca tccacaccgg ggagaaaccg cgacggttca
     7261 gaaacaggat gctctgctca ccctttttaa gccggtccgc gagcgcctcg cgcaacggca
     7321 gcgagagtcc ctcgctcggc ttgagccggc gcatgtcgag caggcgtacc tccggcatga
     7381 ccgcgctccc ggtgcgctcc ggtaactcga tcagtgcata ggcgttttgc ctggcattct
     7441 tgaggctctc cagcgaaggc gtggccgaac cgagaatgat cggtatcttt tcgcgcgcgg
     7501 cgcgcatcac agccacgtca cgcgccgaat aacggaaacc gtcctgctgc ttgtacgagg
     7561 catcgtgctc ttcgtccacg acgataagac cgggattttt cagcggcgtg aagacggccg
     7621 agcgcgtgcc gagcacgatg ggcgccttgc cttccgtcgc cctcagccac gcactcaggc
     7681 gttcgtgttc attaagcccg gaatgcagca ccgcaatcgg cgtgtgaaaa cgtcgctcga
     7741 aacgacccac caattgcggc gtgagactga tttccggcac cagcaccaac gcctgttttc
     7801 cctgcgccag cgcctgctcg attacgctca ggtaaacctc ggtcttgccg ctgccggtaa
     7861 cgccgtgtaa caggaatgga tgaaaacccc ccagcccggc aagcatggcg gtcacggcgg
     7921 atttctgatc cgggttgagc gacggcgcgg cctgcaaatt cccgttcacc acaggctgtc
     7981 gttcacgttg gtgactgctc acccacccct tggcacgcaa cgccttgagc gcggcggcaa
     8041 aacgctgcga tacctgagcg agctgttccg catcgagacc ctccgtggcg gccgccagcg
     8101 cctcgaaaac atgtttttgc gccggggcgc gtttcagcgt ttgcgggtca gccgccctgc
     8161 ctgccggcgt gagcgaccac acctttacgc cgccaacaac cggcgcgtgg ccctgacgca
     8221 accgcaccgg cagggccgta tgtatgactt cgccaacggg atgatggtaa taatccgccg
     8281 cccactggag cagacttagc atggacgaag gcatgatggc tctcgtatcc agcggctggg
     8341 tgatggactt cagccgattg gagggcaact cgcttcgtgc gacttcgccc actactacac
     8401 caaccacttc gcgccggccg aatggcacgc gtacgcgcac ccccggcgcc agcatcacgg
     8461 gagccagata gtcaaatgcg cgatacaaag gggtcggcac ggccacttga taaattcgcg
     8521 acgttgcaga catggcggcg actctatcac aggccgattg gcgtaatttt ttgaacattt
     8581 tctgtgcaat acgtgaaaca ctcgcgctcc acgttcacgg gtttgtcatg aaatgctgct
     8641 aagcgcttgt atctaaacga tccaaacgtt actcacagaa tctgtggata acaatgttga
     8701 tgaagctgta acgcacaatt tgttgcacgg caatttccgg ggttgcgtta atttgattaa
     8761 aaaataaact gacgtattta ttgcttcgcg atcaatacgt taataaaaaa tacccgggtt
     8821 ttattgaggg atgattcgcg cgcaggccac ggtgaaactc agatgcggtt gcgttgtgca
     8881 taaaacagac aaccgccgac ggaatcgtca ggggaaattt gactgttttt tgagcaaccc
     8941 tgtgctggca agcgggaaaa acaagggata cgatgccgcg aaccacggaa aaggagaatg
     9001 aacatgaaag gcgcatgcaa gtgggtgaaa caatacggac cactgatcgg acggctgctg
     9061 ctgtcaaacc tgtttatcgt ttc
//
LOCUS       DBNGZE010000199         6452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1418586, whole genome shotgun sequence.
ACCESSION   DBNGZE010000199 DBNGZE010000000
VERSION     DBNGZE010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1418586"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..708
                     /gene="nth"
                     /locus_tag="AC16FV_05180"
     CDS             73..708
                     /gene="nth"
                     /locus_tag="AC16FV_05180"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR2849488.1"
                     /translation="MTPAKRAEIFRRLKKANPRPTTELKYRSPFELLVSVILSAQATD
                     KSVNKATAELFKVANTPKQIIGLGLRGLKRHIRTIGLYNTKAANILKTCRLLIEKHHG
                     QVPATREELQALPGVGRKTANVMLNTAFGQPTIAVDTHIFRVANRTGLATGKNVLEVE
                     MKLLKAVPPEYRHDAHHWLILHGRYTCIARKPRCANCVIYDLCEYRAKVKA"
     gene            705..1853
                     /locus_tag="AC16FV_05185"
     CDS             705..1853
                     /locus_tag="AC16FV_05185"
                     /inference="COORDINATES: protein motif:HMM:NF021926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HIR2849489.1"
                     /translation="MKPGYVATGLSHGHRALPEHAEKAVKRALAKAGCERANAILLFL
                     TPEYAANPEPALRAAARAGGCLQVVGCTGAGILTEEEWVLDSPGAAAMVLSGDIQLTI
                     SPHTNRNDEDMVLSLCTPAGLTANWLDVPARRFGAVSGDLFGHGPFKVWSGARVSDAE
                     QVDAVLSGVLGVLGASQGVRALTSPIEVGEVQGYDIKRLGNYPALSVLVQSLPPAVRE
                     MERVPLHLIMGGVTIGDPETAIREGRYRLNHIVAANPNDQSITLSERPARGERIFWAM
                     RDALVAERDMLACITRSTRELGTEPDFALLFPCMGRGPNFFGNRDRDLDNLRSRFPGL
                     PVIGMYGNGEIGPLDGVNHLFQYSAVLGLFCCAEKRGRRETQDSAETI"
     gene            1856..2293
                     /locus_tag="AC16FV_05190"
     CDS             1856..2293
                     /locus_tag="AC16FV_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HIR2849490.1"
                     /translation="MLVGKDRDQTREVFFRAWRAHREGRPLEGVEKLIVQVVLRHPEY
                     HPVLEQSETTRERDYFPEAGETNPFMHLGMHIAIEEQLSIDQPPGIRGYYQKLLSRYP
                     DEHTVQHHMMECLGEMLWQASRQATAPQETLYLDCLRRLTSEP"
     gene            2414..2869
                     /locus_tag="AC16FV_05195"
     CDS             2414..2869
                     /locus_tag="AC16FV_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2849491.1"
                     /translation="MALAQSTARLVTPIVKGLDSAHSLIDFVVRLWVANVFWKSGVAS
                     LHDWETTVDLFRYEYQVPLLSPQAAAILGTGVELFFPVLLVVGLATRFSAASLFIFNI
                     IAVISYPALGEAGLKDHIYCGILLLVTLLHGPGKISIDHFIRRKWMGDR"
     gene            2918..3907
                     /gene="arsS"
                     /locus_tag="AC16FV_05200"
     CDS             2918..3907
                     /gene="arsS"
                     /locus_tag="AC16FV_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079748.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR2849492.1"
                     /translation="MSRTAVKIKPVTGPSFATHLDRHGLGPLRRDRLRELQINVGRLC
                     NQACNHCHVDAGPKRTEIMAWGTMEKILDWAKTAGVMAVDITGGAPEINPDFRRFVDG
                     FLAQGMHVTVRCNLTVLTETGQEDLASWYARRKIHLVCSLPCYTRKNVDAQRGDGVFE
                     KSIEALKRLNAAGYGGDETLVLDLVYNPGGAFLPGAQEKLEADYKQRLKEDFGVVFSR
                     LMTITNLPINRFAHFLERTGQHDNYMQLLEQNFNPATVPGLMCRHLISVDWLGRVYDC
                     DFNQMLDIPLGDKPHRHLWDITVEQLAGAPIAVDSHCLGCTAGAGSSCGGALA"
     gene            complement(4026..4652)
                     /locus_tag="AC16FV_05205"
     CDS             complement(4026..4652)
                     /locus_tag="AC16FV_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR2849493.1"
                     /translation="MSQPQLILFARQPIPGQVKTRLQPDYSPERAAEIAAFMIRATVE
                     LAVSAWPGDVTLSVWPGTDHPLFRQLADEFHIRLAPQSDGDLGAKMLDALRAGIVRGE
                     CTAIMGCDVPHCGWDVIDQANDWLARGRNVLGPTEDGGYYFIGLQEARPELFEAMPWG
                     SDRVLEMTLARAEKLGMEFNLLKTLRDIDTASDLWLVAQKYEPLKKFL"
     gene            complement(4802..5509)
                     /locus_tag="AC16FV_05210"
     CDS             complement(4802..5509)
                     /locus_tag="AC16FV_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR2849494.1"
                     /translation="MRVAVILPVLNEAAHLDAVLSALLELQHFDEIIVVDGGSTDASV
                     EIVCKFMSSDEPDAHPVPILIQSPRGRASQMHAGAQAAQADVLLFLHADCTLPPGAAD
                     DIRDAIGQGHAWGRFDVRLSGRHFLFRVIERLMNWRSRFTGIATGDQAVFVRSDVYRV
                     LGGYAPMALMEDIEFSARLKSVGRPVCLPGPVLVSSRRWEKHGIIRTILMMWTLRFLY
                     WLGVSPERLARWYYQRP"
     gene            complement(5817..6056)
                     /locus_tag="AC16FV_05215"
     CDS             complement(5817..6056)
                     /locus_tag="AC16FV_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008029053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HIR2849495.1"
                     /translation="MQWLDKIPLTALVIAALALGLAPLTPEPHLWEKLKMLAHGTLAR
                     PIVIFDLIMHASLPLLLVVRLIRMVRSGNKPGPSA"
     gene            complement(6062..>6452)
                     /locus_tag="AC16FV_05220"
     CDS             complement(6062..>6452)
                     /locus_tag="AC16FV_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2849496.1"
                     /translation="LRRKDYPDGKFTLAFDARHPWRAPLRGALKCAQFHSRRNCVGYG
                     PETEHAVVELTYNWGTGKYDLGNGFGHIALEVDDAYKACADIKTRGGKVTREAGPMKH
                     GSTVIAFVEDPDGYKIELIQRGTQGAR"
BASE COUNT         1319 a         1984 c         1899 g         1250 t
ORIGIN      
        1 gcggagattt ttcgattatt tctgtttttc tctgcgtcct ctgcgtctct gcgtcagata
       61 atcatatcaa tcatgacgcc agccaagcgc gcagaaatct tccgccgcct caaaaaggcc
      121 aacccacgtc cgacgacgga gctgaaatac cgcagcccgt tcgagctgct cgtgtccgtc
      181 atcctctctg cgcaggcgac ggacaaaagc gtcaacaagg ccacggcgga gcttttcaaa
      241 gtcgcgaata cgccaaaaca aatcatcggt ctcggactgc gcggtctcaa gcgtcatatc
      301 aggaccatcg gcctgtacaa caccaaggcc gcgaacatcc tcaaaacctg ccgccttctg
      361 attgagaaac atcatggcca ggttcccgcc acgcgcgagg aacttcaggc cctgccgggc
      421 gtcggccgca agaccgccaa cgtgatgctc aacaccgctt tcggccagcc gaccatcgcc
      481 gtggatactc atatcttccg tgtggccaat cgcaccggac tcgctaccgg caaaaatgtg
      541 ctggaggtgg agatgaagct gctgaaagcc gttccgccgg aatatcgcca cgacgcccac
      601 cactggctga ttctgcacgg gcgttatacc tgcatcgccc gcaaaccacg ctgcgcgaat
      661 tgcgttatct atgatttatg cgagtacagg gctaaagtta aagcatgaaa cccggatacg
      721 tcgccaccgg cttgagtcac ggtcaccgcg cccttcccga gcacgcggag aaagcggtga
      781 agcgcgcgct tgccaaggcc ggatgcgagc gcgccaatgc catcctgctg tttctcaccc
      841 cggagtacgc cgccaatccc gagccggcgt tgcgcgccgc ggcgcgggct ggcggttgcc
      901 tgcaggtggt gggctgcacc ggtgccggca tcctgacgga agaggaatgg gtgctggaca
      961 gcccgggcgc ggccgccatg gtgctgagcg gcgacataca gctgacgatt tctccccata
     1021 ccaatcgcaa tgatgaagac atggtgctga gcctgtgcac gcccgcgggg ctcacggcga
     1081 attggctcga cgtgccggcg cgccgcttcg gcgcggtgtc gggcgatctt ttcggtcacg
     1141 gtccattcaa ggtctggagc ggtgcgcgcg tttcggatgc cgaacaggtg gacgccgtgc
     1201 tcagcggcgt gctcggcgtg ctcggcgcct cgcagggcgt gcgtgcgctg acctcgccca
     1261 tcgaagtagg agaggtgcaa ggttacgaca tcaagcgcct tggcaattat ccggcactga
     1321 gcgtgctggt gcagtcgctg ccgccggccg tgcgtgaaat ggagcgcgta ccattgcacc
     1381 tgatcatggg cggcgtgacc atcggcgatc cggagacggc catccgcgag ggacgttacc
     1441 gcttgaatca catcgtcgcc gccaacccga acgaccagtc catcactctc tcggagcgtc
     1501 cggcgcgggg cgagcgaatt ttctgggcca tgcgcgacgc cctggtggct gagcgcgaca
     1561 tgcttgcctg catcacgcgc agcacgcggg aactcggaac cgagccggat ttcgccctgc
     1621 tgtttccatg catggggcgc gggccgaact tcttcggcaa ccgcgaccgc gacctcgaca
     1681 atctgcgttc acggtttcct ggattgccgg tcatcggcat gtacggcaat ggcgaaatcg
     1741 gaccgctcga cggcgtcaat cacctgttcc agtattccgc tgtcttgggc ctgttctgct
     1801 gcgctgaaaa gcgcggacgt cgcgagacgc aggacagcgc cgaaaccatc tgacaatgct
     1861 tgtcggcaag gaccgcgacc agacgcgcga ggtttttttc cgtgcttggc gcgcgcaccg
     1921 tgaaggacgt cctctcgaag gcgtggaaaa gctgatcgtg caagttgtgt tgcggcaccc
     1981 ggaatatcac ccggtgctgg aacaatccga gacgacgcgc gagcgtgatt attttccgga
     2041 agcgggcgaa accaacccgt tcatgcatct cggcatgcat atcgccatcg aggaacagct
     2101 gtccatcgat cagccgcccg gcatccgcgg ttattatcag aaactcctca gccgctatcc
     2161 ggacgaacac acggtccagc accacatgat ggaatgcctc ggtgaaatgc tgtggcaggc
     2221 cagccgccag gcgaccgctc cccaggagac gctatatctc gactgcctgc gccgactgac
     2281 gtcggagccg tagcggctat cctttcccgg aaacgggcca agccgaaact tttgtataga
     2341 cgccgggtct cattgcctgt ccttgcccga acgcaacaat atcgccaata aagaataccg
     2401 tcaggagaac cccatggcac ttgcccaaag caccgcacgg ctggtaacac ccatcgtcaa
     2461 aggactggat tctgcccatt cgctgatcga ttttgtcgtg cgtctgtggg tggccaatgt
     2521 cttctggaaa tccggcgtgg cttcgctgca cgactgggag accacggttg acctgttccg
     2581 ctacgaatat caggtgccgc tcctgtcgcc tcaggccgcg gcaatactgg gcacgggcgt
     2641 ggagcttttc ttcccggtgt tgctggttgt ggggctggct acccgtttca gcgccgcatc
     2701 cctgttcatt ttcaatatca tcgcggtcat ttcctatccc gccctcggcg aggccggact
     2761 caaggaccac atttactgtg gcattctgct gctggtgact ttgttgcacg gaccgggcaa
     2821 gatttcgatc gatcatttca tccgacggaa atggatgggc gatcggtagc cctactccgc
     2881 ttcgctcatt cgtcttatga taagaattga tctcgacatg tcacgcacag ccgtcaagat
     2941 aaagccggtt accggccctt cgttcgccac ccacctcgac cggcatggtc tgggtccgct
     3001 gcgccgtgac cgcctgcgcg aactgcaaat caacgtcggc cgtctgtgca atcaggcctg
     3061 taatcattgc cacgtggacg ccggccccaa gcgcaccgag atcatggctt ggggcaccat
     3121 ggaaaaaatc ctcgactggg ccaaaacggc cggtgtcatg gcggtggaca tcaccggtgg
     3181 cgcgcccgaa atcaatccgg attttcgccg cttcgtggat ggattcctcg cgcaaggcat
     3241 gcacgtcacc gtgcgctgca atctcacggt gctgaccgaa accggccagg aggatctggc
     3301 gtcatggtat gcgcggcgga aaatccatct ggtctgttcc ctgccctgct acacgcggaa
     3361 aaacgtggac gcccagcgcg gcgacggcgt gtttgaaaaa agcatcgagg ccctgaaacg
     3421 cctgaatgcc gcgggatacg ggggcgacga aacgctggta ctggacctgg tctacaaccc
     3481 cggcggcgcg ttcctgccgg gcgcgcagga aaaactggag gccgattaca agcagcggct
     3541 gaaggaagac ttcggcgtgg tcttctcgcg cctcatgacc atcaccaacc tgccgatcaa
     3601 ccgcttcgcg cacttcctcg aacgcaccgg tcagcacgac aactacatgc aactactgga
     3661 gcaaaacttc aatcccgcga ccgtgccagg attgatgtgc cggcatctca tcagcgtgga
     3721 ctggctcggc cgggtgtacg actgcgattt caaccagatg ctcgacattc cgctgggcga
     3781 caaacctcat cgtcatctgt gggacatcac cgtggaacaa ctggccggcg ccccgattgc
     3841 cgtggactcc cactgcctcg gctgcaccgc cggcgccggg agcagctgcg gtggcgcgct
     3901 ggcttaatgt ccttgacaaa ataaatagac aggattaaca ggatttttca ggattcacag
     3961 gattaaggat ttaatagaag agaaatcctg taaatcctgt ctgatttttt tcttttccta
     4021 gattcttaca gaaatttctt gagcggctcg tatttctgcg ccaccagcca gaggtctgac
     4081 gcggtgtcga tgtctcgcag cgtcttcagc agattgaatt ccatgcccag tttttcggcg
     4141 cgcgccagcg tcatctccag cacgcgatca ctgccccagg gcattgcctc gaacagctct
     4201 ggtcgcgcct cctgcaaacc aatgaagtag taaccgccgt cttcggtggg cccgaggaca
     4261 tttcggccac gcgccagcca atcgttggcc tgatcgatga cgtcccaacc gcagtgcggg
     4321 acatcgcaac ccatgatcgc cgtacattcg ccgcgcacga taccggcgcg caacgcatcc
     4381 agcatcttgg cgccgagatc gccgtccgat tgcggcgcga gccggatgtg aaattcatcg
     4441 gcaagctggc ggaacagcgg gtgatcggtt ccgggccaga cagatagcgt gacatcgccc
     4501 ggccaggcgg aaacggccag ctcgaccgtg gcgcggatca tgaaggcggc aatctcggcc
     4561 gcccgctccg ggctgtaatc cggttgcagg cgggttttga cttggcctgg aatcggctgc
     4621 cgggcgaaaa gaatcagttg cggctggctc atgccactcc tttcgttatt agagtttcta
     4681 acaaaatgaa tatgattttg aaccgccaag tcgctaaggc gccaagaaag gccttttttt
     4741 caacagtaaa aacttggcgt tcttggcgtc ttggcggtta agtttctttt cgttaggggt
     4801 tctaagggcg ctgatagtac cagcgcgcca ggcgctcggg cgaaacaccc agccagtaca
     4861 ggaagcgcag ggtccacatc ataaggatcg tgcggataat tccatgcttt tcccagcgcc
     4921 gcgaggaaac cagcaccgga cctggcaggc acactggccg gccaaccgac tttaggcggg
     4981 cggaaaattc gatgtcttcc atcagcgcca tcggcgcgta gccaccgagc acgcgataga
     5041 catcgcttcg cacaaagacg gcctgatcgc cggtggcgat tccggtgaaa cgcgaacgcc
     5101 agttcatcag gcgttcgatg acgcgaaaca aaaaatgccg tcccgacaaa cgcacgtcga
     5161 aacggcccca cgcatgcccc tgaccgatgg cgtcgcgaat atcgtcggcg gcgccgggcg
     5221 gcaacgtgca gtccgcatgc aggaacaaca atacatccgc ttgcgccgcc tgtgcgcccg
     5281 cgtgcatttg cgatgcccgc ccgcgcggac tttggatcag gatcggcacc ggatgcgcgt
     5341 ccggctcgtc ggaagacatg aatttacaca cgatctccac gctggcatcg gtgctgccgc
     5401 catcaaccac gatgatttca tcgaaatgct gaagctcaag cagcgccgag agaacggcgt
     5461 cgagatgggc cgcttcattc agaaccggca ggatgacggc tactctcatg gcaagacgag
     5521 cgtagcgcag cgaagcaggg ctaccggatc catgacagga cgaacggacc gaagcgcagt
     5581 agggctaccg gtttcatgac aggacgaccg cagcgagtcc gcaatcgcgc ctgctcttat
     5641 gccaggacga gcgaccgaag ggagcagggc ttccttttcg gcgcgcggcc ctcgcctcgt
     5701 tcccatcggc gaagcggatc atgtctcagg ttccatggca aaacgaacgg agcgcagcgt
     5761 agcagggctg ccgtattgag agcaggagaa accgacgcaa ggcagcgcgg cggcgctcag
     5821 gcggaagggc cgggtttgtt tccggaacgc accatgcgaa tcagcctgac aaccagcaat
     5881 aatggcaggg acgcatgcat gatcagatcg aaaataacga tgggccgtgc gagcgtccca
     5941 tgcgccagca tcttgagctt ctcccacagg tgcggctccg gcgtgagcgg cgccagtccc
     6001 agcgcaagtg cggcgatcac gagcgcggtg agcggaattt tatccagcca ttgcatgatg
     6061 actatctcgc gccctgtgtg cctcgttgga tcaattcgat cttgtagccg tccgggtcct
     6121 cgacgaaggc gatgaccgtg ctgccgtgct tcatcggccc ggcttcgcgc gtcacttttc
     6181 cgccgcgcgt cttgatgtcc gcgcaggctt tgtaggcgtc gtccacttcg agcgcgatgt
     6241 ggccaaaacc gttgccgaga tcgtatttgc cggtgcccca attgtaggtc agctcgacga
     6301 cggcatgctc ggtttcggga ccataaccga cacaattccg ccgggagtgg aattgggcgc
     6361 actttagtgc gccgcgaagc ggcgcgcgcc aaggatggcg cgcgtcaaac gcgagcgtga
     6421 acttgccgtc gggataatcc ttccggcgca ac
//
LOCUS       DBNGZE010000200         2974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_932118, whole genome shotgun sequence.
ACCESSION   DBNGZE010000200 DBNGZE010000000
VERSION     DBNGZE010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_932118"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /gene="hisB"
                     /locus_tag="AC16FV_05225"
     CDS             complement(<1..501)
                     /gene="hisB"
                     /locus_tag="AC16FV_05225"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR2849497.1"
                     /translation="MSSRRAEVTRNTKETQITLSVNLDGSGASRLNTGLPFLEHMLEQ
                     VARHGLIDIVLEAKGDLHIDAHHTVEDIGITLGQALAKAVGDKKGIRRYGHAYVPLDE
                     ALSRVVIDFSGRPGLEYHVDFPRSRVGEFDVDLVHEFFQGFCNHALVTLHVDCLRGKN
                     AHHNAET"
     gene            complement(561..1646)
                     /gene="hisC"
                     /locus_tag="AC16FV_05230"
     CDS             complement(561..1646)
                     /gene="hisC"
                     /locus_tag="AC16FV_05230"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR2849498.1"
                     /translation="MVDTIRKKVEALVRPEIQALKAYHVPDATGLIKLDAMENPYSWP
                     EPMKREWFDALQQVALNRYPDPEARRLRERLRAALGIPLGMELVLGNGSDELIQIILM
                     AVARPGAVVLAPTPTFVMYEMIATFTGMKFVGVPLAPDFSLDMPAMRKAIERHQPAVI
                     FLAYPNNPTGNLFAREDIVRVIERTPGLVVLDEAYHAFAGQSFMDQLGKHDNLLVMRT
                     LSKQGLAGLRLGVLAGPRPWLEEFNKVRLPYNIGVLTQASAEFALAHLALMEEQAGLI
                     RAERERVFESLSALKDVRAWPSRTNFILFRVEKGDADRVFEGLCQRGVLIKNLNSAGG
                     VLGNCLRVTVGTPEENNSFLKALEASL"
     gene            complement(1745..>2974)
                     /gene="hisD"
                     /locus_tag="AC16FV_05235"
     CDS             complement(1745..>2974)
                     /gene="hisD"
                     /locus_tag="AC16FV_05235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR2849499.1"
                     /translation="AQATDPKVETTVKEIIANVRARGDEALLDYTRRFDRRELSSAAE
                     LEIPRTRLREAARALNKDQYEALANAAERIRAYHERQRSESWSYTEPDGTRLGQQVTP
                     IERIGLYVPGGKAAYPSSVLMNAIPAKVAEVHELVMVVPAPEGELNPLVLGAAHLGGV
                     DRVFTIGGAQAVAALAYGTASIPRVDKIVGPGNIYVATAKRLVFGAVDIDMIAGPSEI
                     VVLCDGQTDPEWIAMDLFSQAEHDEDAQSILISLDEAYTAKVRAAIEKLLPGMERRDI
                     IRTSLEARGALITAANLDEALALVNRIAPEHLELSVENPEDAAGRVRHAGAIFLGRYT
                     AEAVGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRTSIIQCSADGASILGHTASVLAR
                     GEGLTAHARSAEYRIKK"
BASE COUNT          571 a          929 c          912 g          562 t
ORIGIN      
        1 ggtctcggca ttgtgatgcg cgttcttgcc gcgcaggcaa tccacgtgca gcgttaccaa
       61 ggcgtggttg cagaagccct ggaaaaattc gtgcaccagg tccacgtcga attcgcccac
      121 gcgcgagcgc gggaaatcca cgtgatattc caggccggga cgtcccgaga agtcgatgac
      181 gacgcgcgag agtgcctcgt cgagcggcac gtaggcgtga ccataacggc ggatgccctt
      241 cttgtctccc accgccttgg cgagcgcctg acccaaagta atgccgatgt cctcgacggt
      301 gtggtgtgcg tcgatatgca ggtcgccctt ggcttccagg acgatgtcga tcaggccatg
      361 ccgcgccacc tgctccagca tatgctcgag aaacggcagg ccggtattga ggcgcgacgc
      421 accgctgccg tccaggttca cggacagggt aatctgggtt tccttggtgt tgcgcgtgac
      481 ctccgcgcgc cggctagaca cgaagtttct ccgggggatt cgtgaatttt atggtatcac
      541 accaataata catagacgaa tcagagtgac gcctcaagcg ccttgaggaa ggaattgttt
      601 tcctcgggcg tgccaacggt cacgcgcagg cagttcccga gcacgccacc ggcggaattc
      661 aggttcttga tcagcacgcc acgttggcac aacccttcga agaccctgtc tgcatcaccc
      721 ttctccacgc ggaacaggat gaaattggtc cgactcggcc aggcccgcac atctttcagg
      781 gcgctcagcg attcaaagac gcgttcacgt tcggcccgta tcagtcccgc ctgctcctcc
      841 atgagcgcga gatgcgccag cgcaaattca gcgctggcct gcgtcaggac gccgatgttg
      901 tagggcagcc tgaccttgtt gaattcctcg agccagggtc gcggacccgc gagcacgccg
      961 aggcgcagcc cggcgaggcc ctgtttggag agcgtgcgca tcaccagaag attgtcatgc
     1021 ttgcccaatt gatccatgaa gctctggccg gcgaaggcat gataggcctc gtccagcacc
     1081 accagccccg gcgtgcgttc aatcacccgc acgatgtcct cgcgcgcgaa cagatttccg
     1141 gtaggattgt tcgggtaggc aagaaaaatc accgccggct ggtgcctctc gatcgccttg
     1201 cgcatggccg gcatgtcgag tgaaaaatcc ggcgccagag gaactccgac gaatttcatg
     1261 ccggtgaacg tcgcgatcat ctcgtacatc acgaacgtcg gcgtcggcgc cagcaccacc
     1321 gcaccgggcc tggccacggc catcagaata atctgaatca gctcatccga accgttgccc
     1381 aacaccagct ccatgcctaa cgggatgccg agcgccgcgc gcagacgctc gcgcaagcga
     1441 cgggcttcgg ggtcgggata gcggttgagc gccacctgtt gcagcgcatc gaaccattcg
     1501 cgcttcatcg gctccggcca ggaataggga ttttccatgg cgtcgagctt gatgaggccg
     1561 gtggcgtccg gcacgtgata ggccttcaat gcctggattt caggccggac caaggcttcg
     1621 actttttttc gaatcgtatc gaccatataa ataagaagtt aaaccgccaa ggcgccagga
     1681 tcgccagggt aacaaaatta attgaaatct tttcttggcg tccctggcgt cctggcggtt
     1741 caaactattt tttgatccgg tattccgccg agcgtgcgtg cgccgtcagg ccctcgccgc
     1801 gcgcgagcac ggaagcggtg tggccgagga tggaggcgcc gtcggccgag cactggatga
     1861 tgctggtgcg cttctggaaa tcgtaaacgc ccagcggcga ggagaaacgc gcggtgccgg
     1921 aggtcggcag cacgtgattc ggtccggcgc agtaatcgcc caccgcctcc gcggtataac
     1981 gcccgagaaa aatcgcgccg gcgtgacgca cgcgcccggc ggcgtcttcg ggattttcca
     2041 ctgaaagttc gagatgctcg ggcgcgatgc ggttcacgag tgccagcgcc tcatcgagat
     2101 tcgcggcggt gatcagcgcg ccgcgggctt cgagcgaggt ccggatgatg tcgcgccgtt
     2161 ccatgccggg tagcagcttt tcaatggccg cccgcacttt cgcggtgtag gcctcgtcga
     2221 ggctgatgag aatggattgc gcatcctcgt cgtgctccgc ctgcgagaac agatccatgg
     2281 cgatccactc gggatcggtt tggccgtcgc acagcaccac aatctcggaa ggaccggcga
     2341 tcatatcaat gtcgaccgcg ccgaacacga gccgcttggc cgtggccaca tagatattgc
     2401 cggggccgac aatcttgtcc accctgggaa tggacgccgt gccgtaggca agcgccgcca
     2461 ccgcctgcgc accgccgata gtgaacacac ggtcgacacc acccagatgc gcagcaccga
     2521 gcaccagcgg gttgagctcg ccctccggcg ccggcaccac catcaccagt tcatgcacct
     2581 cggcaacctt cgccgggatc gcgttcatga gcaccgacga gggataggcg gccttgccgc
     2641 cgggaacata aagaccaatg cgctctatcg gcgtcacctg ttgcccaagc cgggtaccat
     2701 cgggttcggt atagctccag gattcggagc gctgacgctc gtgataggcg cggatgcgct
     2761 cagcggcgtt ggccaacgcc tcgtactggt ccttgttgag tgcgcgcgcc gcttcgcgca
     2821 ggcgcgtgcg aggaatctcg agttccgccg cggaggataa ttcgcggcga tcgaaacggc
     2881 gggtgtaatc gagcaaggcc tcatcgccgc gggcacgcac gttcgcgatg atttccttca
     2941 ccgtcgtctc gactttcgga tcggtcgctt gggc
//
LOCUS       DBNGZE010000201         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_445719, whole genome shotgun sequence.
ACCESSION   DBNGZE010000201 DBNGZE010000000
VERSION     DBNGZE010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_445719"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="AC16FV_05240"
     CDS             complement(<1..974)
                     /locus_tag="AC16FV_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2849500.1"
                     /translation="MNRMKKNVWLLSFCQALMNSGNILLVATSPLVGYLLAANKALAT
                     LPLGLQYLGTMLTTIPASIFMKHVGRRAGFIVGTLLGLAGAALAAWAIVHGSFFFFCA
                     GAVLFGVFNGFGTYYRFAAADVATDAYRPTAISYVMAGGVIAAVVGPNLAKWSIGLLP
                     SAEFAGSYLALAGLYVASLAVLLFLDIPRPSEHEAHDTGRSLRAIAAQGKFVVAVLGA
                     AVGYGIMSLVMTATPLAMHAHAHPFHDTAFVIEWHVLGMFAPSFATGHLIRRFGVLNI
                     MLTGAVLNALCVVVNLGGTDLTHFWGALVLLGVGWNFLFVGGTTLLTET"
     gene            1261..2256
                     /locus_tag="AC16FV_05245"
     CDS             1261..2256
                     /locus_tag="AC16FV_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR2849501.1"
                     /translation="MLKTLLVYLHLLATAFALVELLKFDIRLLLNLNRPLAAVDLQRL
                     ISTKNTVTLALVVLWVTGIWFVVNGAIDEPGKYLTNQKLWMKVFTVSLLTVNGMLMHS
                     IGFTRLRPGAVFSEMALRDQWLLLGMGVVSSVSWMFAAFLGIARAWNYTASFDDLLVM
                     YLWFLWGGFTVANLPLLYLRLRHNNARVTDTLKFNLLKTLSLFDGFSDDTLWEFLHLT
                     EWHRFKPEQTLIEEGKAGRSFYILANGEASVEKNGKPIGVLKVGDYFGDIAYVREQAA
                     ARAATVTARSEAVVIRIKSKTLTTASDDLRFSLHRAFLRTLDDRPASTDYQLAAP"
BASE COUNT          467 a          705 c          706 g          399 t
ORIGIN      
        1 gtctcggtga gcagcgtcgt cccgccgacg aacaggaaat tccagcccac gcccagcagc
       61 acgagcgcgc cccagaaatg cgtcaggtcc gtgcccccca ggttcaccac cacgcacagg
      121 gcattcagca ccgcgccggt cagcatgatg ttgagcacgc cgaagcggcg gatcagatgg
      181 ccggtggcga aggatggcgc gaacatgccg agcacgtgcc attcgatcac gaacgcggtg
      241 tcgtgaaacg ggtgcgcgtg ggcgtgcatg gcgagcgggg tggccgtcat caccagcgac
      301 atgatgccat aaccgacagc cgcgccgagc acggccacca cgaactttcc ctgcgcggcg
      361 atcgcgcgca gcgaccggcc ggtatcgtgc gcctcgtgtt cactcgggcg cgggatgtcg
      421 aggaagagca gcaccgcaag cgaggcaacg tagaggcccg cgagcgcgag gtagctgccg
      481 gcgaactccg ccgacggcag cagcccgatg ctccacttcg cgagattcgg tcccaccacg
      541 gcggcgatca cgccgccggc catgacatag gaaatggcgg tcgggcggta agcgtcggtc
      601 gcgacatccg ccgcggcgaa acggtagtag gtcccgaaac cgttaaacac gccgaacagc
      661 acggcgccgg cgcagaaaaa gaaaaatgag ccgtgcacga tagcccaggc cgccagcgcc
      721 gcgccggcca gaccgagcag ggtaccgacg atgaaacccg cgcgccggcc gacatgcttc
      781 atgaatatcg aggccgggat ggtggtcagc atggtaccga ggtactgcag gccgagcggc
      841 agggtcgcga gcgccttgtt cgcggcgagg aggtaaccca cgagcgggga cgtggcgacg
      901 agcagaatat tgccgctgtt catgagcgcc tggcagaacg acagcagcca gacgtttttc
      961 ttcattcggt tcatgcaagc actttaaagt cgggattgtg ggcagagtct aacccggctt
     1021 ccgcgcgggc aagaaatcgc gcacgactca ccattaatcg gctgtaatgg cctttttccc
     1081 gacgaaaacg ccacatcgcc cgaccaacaa acaccgccgc aaacggtccg gaccgtatag
     1141 ttgctgtccc tgcttataga aacggtgcgt taacgttgtc cgcgacactg tcgaattgat
     1201 cagccccgaa ttaccggccg caaggaagca atgcaattct tatttgcctc aggaggaatc
     1261 atgctcaaaa ccctgctcgt ttatcttcat ctgcttgcga ctgcctttgc gctggtcgag
     1321 ttgctcaaat tcgacattcg cctgctgctc aacctgaacc ggccgctcgc ggccgtcgac
     1381 ttacaacggc tgatcagcac caagaatacc gtcacgctgg cactggtcgt gctgtgggtt
     1441 acgggtatct ggttcgtggt caacggagcg atcgacgagc ccggcaagta cctgaccaac
     1501 cagaagctgt ggatgaaggt gttcacggtc tcgctgctga ccgtgaacgg catgctgatg
     1561 cattcgatcg gcttcacgcg tctgcggcct ggcgcggtat tcagcgaaat ggcgctgcgg
     1621 gaccagtggc tcctgctcgg catgggcgtg gtgtcgtcgg tgtcgtggat gttcgccgcg
     1681 ttccttggca tcgcccgggc gtggaattac accgccagtt tcgatgacct gctcgtcatg
     1741 tacttgtggt ttctgtgggg cggtttcacc gtcgccaatc tgccgttgtt gtatctgcga
     1801 ttgcgccata acaatgcgcg cgtgacggac acgctcaagt tcaatctgct caagacgcta
     1861 tcgctgttcg acggattcag cgacgacacg ttgtgggaat tcctgcacct gacggaatgg
     1921 catcgcttca agccggagca gacactgatc gaggaaggca aggccggtcg ctcgttttat
     1981 attctggcga acggcgaggc cagcgtggag aagaacggca aacccatcgg cgtgctcaag
     2041 gtcggcgact acttcggcga catcgcctac gtccgcgagc aggccgcggc gcgcgccgcc
     2101 accgttaccg cccggagcga ggcggtggtg atcaggataa aaagcaaaac cctgaccacg
     2161 gccagcgacg acctgcggtt ttccctgcat cgcgccttcc tgcgcaccct ggacgatcgc
     2221 ccggccagca ctgactacca gctggccgcg ccctgaaccc gataaagaaa cgcccgg
//
LOCUS       DBNGZE010000202         7738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1487949, whole genome shotgun sequence.
ACCESSION   DBNGZE010000202 DBNGZE010000000
VERSION     DBNGZE010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1487949"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="AC16FV_05250"
     CDS             complement(<1..836)
                     /locus_tag="AC16FV_05250"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR2849502.1"
                     /translation="MLTLMPLEQDIASQFVLDGKISAIRPHGQGLINDTFVVTTDTGT
                     QVILQRINRHVFPHPERIMENLRVLTAHIRQHAASDTEGRTLRLPEILTAHNGRDFVI
                     DADGGFWRSQEFIEHTRTLDTISDIAQAEEVGFALGRFHSLIHDLDPARLHRTLPGFH
                     DAPGYFTRFLRASARPRQTGASHDLLYGLSFVERRWSLTRVLEQARREGKLATRAIHG
                     DTKLNNFLFDTRTGKAVSLIDLDTVQPGLIHYDIGDCLRSCANPAGESPGDIAAIRFD
                     PD"
     gene            1138..1761
                     /locus_tag="AC16FV_05255"
                     /pseudo
     CDS             1138..1761
                     /locus_tag="AC16FV_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            1945..2151
                     /locus_tag="AC16FV_05260"
     CDS             1945..2151
                     /locus_tag="AC16FV_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849503.1"
                     /translation="MRDKEGANFCDWFKPRPEAHRSRGGEKTQAVKARLDDLFGGAQT
                     SGDKTEAAWDKLSDLFAPDKKPGK"
     gene            2165..2662
                     /locus_tag="AC16FV_05265"
     CDS             2165..2662
                     /locus_tag="AC16FV_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HIR2849504.1"
                     /translation="MYRIGECRDCEALNATLRARIELLTPGEIVRRHHYLGGRYENLY
                     VMIESIPELRVILDLATAQAAHILNRPKKDLRLGWWLNIMQPGDITYPHTHDEGDELL
                     SGVYYIDVPPHSGELLLTHGAQRLEIEPRAGRFVFFAPDILHEVKRNESDRPCISVGF
                     NVGPA"
     gene            2715..3073
                     /gene="rnpB"
                     /locus_tag="AC16FV_05270"
     ncRNA           2715..3073
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16FV_05270"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3348..3812
                     /gene="mraZ"
                     /locus_tag="AC16FV_05275"
     CDS             3348..3812
                     /gene="mraZ"
                     /locus_tag="AC16FV_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR2849505.1"
                     /translation="MFRGVNTLSLDSKGRMAIPTRYRDQLARACNGQMVLTINALDSD
                     RCLLLYPQPEWEEIERKIMKLSSFNKQTRKLQRMLVGHATECEMDGNGRILLTPPLRE
                     FSGLNKEIVLIGQGNKFEIWDEGTWNTRRGEWLEGEGKGDDKLPPDLESLSL"
     gene            3822..4772
                     /gene="rsmH"
                     /locus_tag="AC16FV_05280"
     CDS             3822..4772
                     /gene="rsmH"
                     /locus_tag="AC16FV_05280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR2849506.1"
                     /translation="MNEHHHVPVLLEEALQALQVKEDGVYVDATYGRGGHAQEILKKL
                     GERGRLLVFDRDPHACEDARRRMGNDQRVQIHQGPFSTIERMIESLGLTGSINGILFD
                     LGVSSPQLDDPQRGFSFRTEGPLDMRMDPRTGESAAEWINRAEENEIVRVLRDYGEER
                     FARRIARAILRERSIEPIATTRRLAEIVARAVPTREKSKDPATRTFQAIRIHINQELE
                     ELMTALPQAVQALATGGRLAVISFHSLEDRVVKHFLRTEAKGRELPPGLPVRHDQFRP
                     RLKIVGKAIRAGATETDRNPRARSAVLRVAERTEEATAHA"
     gene            4765..5043
                     /gene="ftsL"
                     /locus_tag="AC16FV_05285"
     CDS             4765..5043
                     /gene="ftsL"
                     /locus_tag="AC16FV_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR2849507.1"
                     /translation="MPRRLVILFAAVIVVSIAVVQTRHESRQRFAQLQAQQAERDALN
                     VEWGKLLLEEGAWSQHRRVEIMARSRLNMSTPEAANIRVVRVMPDSAP"
     gene            5040..6755
                     /locus_tag="AC16FV_05290"
     CDS             5040..6755
                     /locus_tag="AC16FV_05290"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR2849508.1"
                     /translation="MSRRAKSRYPVRNAIVLLFFLGGLVLLAARAVYLQVLNSDFLQS
                     QGNQRHSRLVKDNSHRGMILDRHGSPLAISTPVDSVWAHPPTLAEERRKWPRLAALLE
                     MSPRELAQLMRRHEGREFMYLKRHVPPPLAERVMALKIPGVALQREYRRYYPLGSVAG
                     HVIGFTNIDDQGQEGVELAYDASLRAVPGTKRLLRDLQGNAVEVAESVVLPKPGKDMV
                     LSIDRRIQYLAYRELKAAVFEHGARAGSAVVLDARTGEVLALVNEPDFNPNNRAGLRS
                     GMFRNRAVTDLFEPGSTLKPFTIACALESGKYSPGSIIDTTPGVLQVGDRTFRDVHNY
                     GVLTVAGVIEKSSNVGVSKIALALNKKSMWEMLRHAGFGQTTGAQLPGEVAGLLNPYS
                     NWVPVDQASISFGYGISVTPLQLARAYLALANDGMALPLTLQHSETTPEGERIMSSKT
                     ARRLQSMLELAVSDAGTGKAAQVADYRVAGKTGTVRKLTSNGYSEDKYVAWFAGFAPV
                     NSPRLVMVVTVDEPAHGAYYGGEVAAPVFGHVMAGALRLLDIPPDAPRKETRLMTVKN
                     EQVAQ"
     gene            6752..>7738
                     /locus_tag="AC16FV_05295"
     CDS             6752..>7738
                     /locus_tag="AC16FV_05295"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR2849509.1"
                     /translation="MTRQVTDLRELLEGMARVPGVANREVRGLQTDSRRVRPGDLFLA
                     VPGMVADGREFIGDAVASGAGAVAYETDDGYVLRGAPVPVFGISGLSRKIGIIADRFH
                     GSPSRRLVVIGVTGTNGKTTCTQLLVQALDQPPRRCAVIGTLGSGFPGALNASIHTTP
                     DAVTVQRLMADFLGQGAAHVAMEVSSHALEQGRVNGVLFHVAVFTNLTRDHLDYHGDM
                     TAYGLAKARLFAYEGLKYAVINHDDDFGRRLLADVGAPVTCLSYGIEGGDVRAREVRP
                     SPEGLWLRVETPYGETELRSPLIGHFNVYNLLAVLATLLALGVELRDAAARLA"
BASE COUNT         1554 a         2196 c         2466 g         1522 t
ORIGIN      
        1 atatcgggat cgaagcgtat tgcggcaatg tcaccgggcg actcaccggc ggggttggca
       61 caggagcgca ggcagtcgcc gatatcgtaa tgaatcagcc ccggttgcac ggtatcgaga
      121 tcgatcaggc ttaccgcctt gccggttcgt gtgtcgaaaa ggaaattatt gagcttggta
      181 tcaccgtgga tcgctcttgt cgccagtttt ccctcgcgcc tggcctgctc caggacacgc
      241 gtgagactcc aacgcctttc cacgaatgac aggccataaa gcagatcatg gctcgcgccg
      301 gtctggcgtg gccgggccga ggcccgcagg aagcgcgtaa aataacccgg cgcatcgtga
      361 aagcccggca gcgtccggtg cagccgcgcg ggatcgagat cgtggatcag gctatggaag
      421 cggccgagag cgaaaccgac ctcttcggcc tgcgcgatat cgctgatggt atcgagcgtg
      481 cgcgtatgtt cgataaattc ctgcgaccgc cagaaaccac catcggcatc aatgacaaaa
      541 tccctgccgt tatgggccgt caatatttct ggcagacgca gcgtgcgccc ttcagtgtcg
      601 ctggcggcat gctggcgaat atgggccgtc aagacacgca ggttttccat gatgcgttcg
      661 ggatgcggaa agacgtggcg gttgatccgt tgcaggatga cctgggtgcc ggtatcggtg
      721 gttacgacaa aggtatcgtt gatcaacccc tgcccgtgcg ggcggatggc gctgattttt
      781 ccatcgagaa cgaattggct ggcgatgtcc tgttcaagcg gcataagggt gagcacgttt
      841 tggcattccg gtacttgtgt tcgggcgttt gacgggcgaa cccgcgcggc attaggctga
      901 gtctagccgc tgggacattt ctgtgaagtg ttctggtggt attgaatcaa tagccaggac
      961 aaggagaagg atcatgcgga gatgggcatt aactgttctg atgtttttta tcggcttggc
     1021 ttcggctcag gccgctatca agggtaatgt ggtcgaatac aaggccggcg acacggcgct
     1081 gcggggttat ctggtttatg acgacgccgt gaagaccagg cgccgggcgt gctggtggtg
     1141 catgaagggt ggggacataa cgaacatgcg cgcaacagcg cccgcaagct cgcggaagcg
     1201 ggttacgtgg cgctggcgct cgacatgtac ggcgatggca agtaggcgaa ccatcccgac
     1261 caggccggaa aattctccgg cgaggtgggg aagaatctgc cactcatgaa atcgcgtttt
     1321 gaggccggca tgaagctgct gcgcaagcag gcgcaggtcg atgccaagcg cgtggccgcg
     1381 atcggttatt gtttcggcgg cagtgtcgtg ctccagatgg cgcgcgaagg cgaaaatctg
     1441 cgcgccgtgg cgagctttca cggcgggctc gcggccgagc atccggccaa gcccggcaag
     1501 gtcaaagcca aagtactggt gatgaacggc gcggaagacc ccttcgtgcc ggcggagcag
     1561 attgcggctt tcaagaagga aatggaaagc gccaaggtcg actacaagtt cgtgaactat
     1621 ccgggcgcga agcacagctt caccaacccg gacgcggata ccaacggcgc caagttcaat
     1681 atgccgcttt cctataacgc caaggccgac caggaatcct ggaccgagct gcaatccttc
     1741 ctcaagcttg cctttaaata attgatgcga ttcgcgaccg atctggtttg ctggaaatgt
     1801 ggtgcttcgc tggcagaact gcccctgcct ttgagtcggc tcgcggaatg tccgaagtgc
     1861 cgcgcctatt tgcacagttg tcgtctgtgt catttctaca atccgcggtt gacgagtcag
     1921 tgtgacgaag agcgcgctga agaagtgcgt gacaaggaag gtgcgaattt ctgcgattgg
     1981 ttcaagcccc gtcccgaggc gcaccgttct cgcggtgggg aaaagacgca ggccgtcaag
     2041 gccaggctcg atgatctgtt tggcggtgcg caaacctccg gcgacaagac ggaggcagca
     2101 tgggacaaac taagtgatct ttttgccccc gacaaaaaac ccggaaaata atgcaattcc
     2161 cccgttgtat cgaatcggtg agtgtcggga ttgtgaggcg ctgaatgcca cgctgcgcgc
     2221 gcgcatcgaa ctgttgacgc cgggtgaaat tgtccgccgc catcattatc tgggtgggcg
     2281 ttatgaaaat ctttatgtaa tgatcgagtc gatcccggag ctgcgcgtga tcctggatct
     2341 ggcgacggca caggccgcgc atatcctgaa tcgcccgaaa aaagatctgc gcctggggtg
     2401 gtggctcaac atcatgcagc cgggcgatat tacttacccg cacacgcacg atgagggaga
     2461 cgaactgctc tccggtgttt attacatcga cgtgccgccc cattccggcg aactgttgtt
     2521 gacacatggc gcgcagcgcc tggaaattga gccgcgtgcg ggaaggttcg tgtttttcgc
     2581 gccggatata ctgcacgagg tcaagcgtaa cgaatccgac cgcccatgca tatccgtcgg
     2641 ttttaacgtc gggccggcat agcgcaggca aggtttccgg aaacaaggca gcgacgactg
     2701 gtataatagt cgccggagtt ggccagacag ccgctgcctg ggcacgttcg cgtggccggg
     2761 tggaggaaag tccgggctcc acagggcagg gtgccaggta acacctgggc ggcgcgagcc
     2821 gatggaaagc gccacagaaa atacaccgcc gaagctcgca agagctggta agggtgaaat
     2881 ggtgcggtaa gagcgcaccg cacgggtggc aacatcccgt ggcagggcaa gccccacccg
     2941 gagtaaggcc aaatagggga acgatagtgc tgcccgcact gttcccgggt aggccgcatg
     3001 aggcgtctgg cgacagacgt cccagatgaa tggctgtcca cgacagaacc cggcttaccg
     3061 gccaactcct tttttcacat taacttagtc gtttaatttt ttgtttttaa tccatttttt
     3121 agtctcactt tcagttacaa atagtgtcac ttttggtctc aaaagtgcgc ggtggtgcat
     3181 tcgtccctcg aaattgcacc tttcatcact aactccctgt cctgcaaaga agaaattttt
     3241 cagaaatgac agttttgtct tgacactcct tattatcgct atctatagtg tcaataagtg
     3301 gataaatgtg gttttttgtg gacgagagcg ggaaataacg gataaaaatg tttcgcggtg
     3361 tcaacacatt gtcgctcgac agcaaaggcc gcatggccat cccgactcgt tatcgcgatc
     3421 agctggcgcg cgcgtgcaac ggccagatgg tactcaccat caatgcgctc gacagtgatc
     3481 gctgcctgct gctgtatccg cagccggagt gggaggaaat cgaacgcaag atcatgaagc
     3541 tttcgagctt caacaagcag acgcgcaagc tgcagcgcat gctcgtcggt cacgccacgg
     3601 aatgtgaaat ggacggcaac ggccgcattc tcctgacgcc gccgttgcgc gagttttcgg
     3661 gtcttaacaa ggagattgtg ctcatcggtc agggaaacaa attcgaaatc tgggacgagg
     3721 gcacatggaa cacgcgtcgc ggtgaatggc tcgaaggtga aggcaagggc gatgacaaat
     3781 tgccgccgga tctcgagtcg ctctcgcttt agcaagaaag cgtgaatgaa catcaccatg
     3841 ttccggtact gctggaggag gccttgcagg ctctacaggt gaaggaagac ggagtgtatg
     3901 tggatgccac ttatggacga ggcgggcacg cgcaggaaat tttgaaaaaa ctcggtgagc
     3961 ggggacggct gctggtgttt gaccgggatc cgcacgcctg cgaagacgcg cgccgccgga
     4021 tggggaatga ccaacgtgtg cagattcacc aaggtccgtt ctcgacaatt gagcggatga
     4081 tcgaaagtct gggtctcacg ggaagcatca acggcattct tttcgatctt ggtgtgtcct
     4141 cgccgcagct cgacgatcct cagcgtggtt tcagtttccg caccgaaggc ccgctcgaca
     4201 tgcgcatgga cccacgcacc ggcgaatccg ccgcggaatg gatcaatcgt gcggaggaga
     4261 atgaaatcgt gcgtgtccta cgcgattacg gcgaggaacg cttcgcccgg cgcatcgcgc
     4321 gcgccatcct gcgcgaacgt tccattgagc ccatcgcaac cacgcgccgc cttgccgaga
     4381 tcgtcgctcg tgccgtgccc acgcgcgaga agagcaagga tccggcaaca cgtacatttc
     4441 aggctattcg cattcatatc aatcaggagc ttgaggaatt gatgacagcg ctcccgcaag
     4501 cagtgcaggc gcttgcgact ggcgggcggc tggcagtgat cagttttcat tcgctcgaag
     4561 accgggtggt gaagcatttt ttgcgtaccg aggcaaaagg tcgcgagctt ccgcccgggc
     4621 taccggtgcg tcacgatcaa ttccgcccgc gcctgaagat cgtcggcaaa gcaatccgtg
     4681 ccggcgcgac agaaactgat cgcaacccgc gtgcccgcag tgccgtgctg cgtgtggccg
     4741 agcgtactga agaggcaact gcccatgcct aggcgcctag ttattctttt cgcagcggtg
     4801 atcgtggttt ccatcgcggt ggtgcagacg cggcatgaaa gccgtcaacg ctttgcgcaa
     4861 ttgcaggcac aacaggcgga gcgggatgcg ctgaatgtcg agtggggcaa gctgctgctc
     4921 gaggaaggcg catggtcgca gcatcgccgc gtcgagatca tggcgcgcag tcgcctgaac
     4981 atgagcacgc ccgaggccgc gaacatccgc gtcgtgcggg tgatgccgga ttccgcgcca
     5041 tgagtcgccg cgccaaaagt cgttacccgg tgcgcaacgc catcgtgctg ctgttcttcc
     5101 tgggcgggct cgtcctgctg gcggcacgcg cggtatacct gcaggtgctc aattccgatt
     5161 ttctccagag ccagggcaat cagcgtcatt cgcgcctggt caaggacaac tcgcaccggg
     5221 gtatgattct cgatcgtcac ggttcgccgc tggccatcag cacgccggtg gattcggtat
     5281 gggcgcatcc gccgacactg gccgaggagc gccggaaatg gccgcgcctg gcggcgttgc
     5341 tcgagatgag cccgcgcgaa ctggcgcagc tcatgcgccg gcacgagggc cgcgagttca
     5401 tgtacctcaa gcgccatgtg ccgccgccgc tggccgaacg cgtgatggcg ctcaagattc
     5461 ccggcgttgc actgcagcgc gaataccggc gctactatcc gttgggttcc gtcgccggcc
     5521 atgtgatcgg tttcaccaat atcgacgatc agggccagga aggcgtcgag cttgcctatg
     5581 atgcctcgtt gcgcgccgtg cccggcacca agcgcctgtt acgagatctg cagggcaatg
     5641 cggtcgaagt cgccgagagc gtggtgttgc ccaagccggg caaggatatg gtgctttcca
     5701 tcgatcggcg catccagtat ctcgcttatc gcgaactcaa ggcggccgtc ttcgagcatg
     5761 gcgcgcgcgc cggcagtgcc gtggtgctgg atgcgcgcac cggcgaggtg ctggcgctgg
     5821 tcaatgaacc cgatttcaat cccaataacc gcgccggcct gcgcagcggc atgttccgca
     5881 atcgcgccgt gacggatctc ttcgagccgg gctcgaccct caagcctttc accatcgcat
     5941 gcgcgctgga gagcgggaaa tattcacccg gcagcatcat cgacaccaca ccgggcgtgt
     6001 tgcaagtcgg tgatcgcaca ttccgtgatg ttcacaacta cggcgtgctg acggtggcgg
     6061 gcgtgatcga gaaatcgagc aacgtcggcg tttccaaaat cgcgctggcg ctgaacaaga
     6121 agagcatgtg ggagatgctg cgtcacgccg ggtttggaca aactaccggc gcgcaacttc
     6181 cgggcgaagt ggcggggctg cttaatccct attcaaactg ggtgccggtg gaccaggcga
     6241 gcatttcctt cggttacggt atttccgtaa cgccattgca gctcgcgcgc gcctatcttg
     6301 cgctcgcgaa cgatgggatg gcgctgccgc tgacattgca gcattcggaa accacgccgg
     6361 aaggcgagcg catcatgtcc agcaaaaccg cgcgccgtct ccagtccatg ctggagctgg
     6421 ccgtgagtga cgccggcacc ggcaaggcgg cgcaggtggc ggattaccgc gtggccggca
     6481 agaccggcac ggtgcgcaaa cttacctcga acggatattc cgaggacaag tatgtcgcct
     6541 ggttcgccgg ttttgcgccc gtcaattcac cgcgcctggt gatggtggtg acggtggacg
     6601 aacccgcgca cggcgcttat tacggcggcg aggtggccgc gccggtgttc ggtcacgtga
     6661 tggccggcgc gttgcgcctg ctcgacatcc cgcccgatgc accgcgcaag gagacgcgcc
     6721 tcatgacggt gaaaaacgag caggtggctc aatgacgcgc caagtgaccg acctgcgtga
     6781 attgctcgaa ggcatggcgc gcgtgccggg cgtggccaac cgcgaggtgc gcggcctgca
     6841 aacggacagc cggcgcgtgc ggccgggcga tctgtttctg gcggtgccgg gcatggtagc
     6901 ggacggacgc gagttcatcg gcgatgccgt ggcctcgggc gccggtgcgg tggcctacga
     6961 aacggacgat ggctatgtcc tgcgcggcgc gccggtgccg gtcttcggca tatccggtct
     7021 gagccggaaa atcggcatca tcgcagaccg ctttcatggc tcgccctcgc gccgtctggt
     7081 ggtgatcggc gttaccggca ccaacggcaa gacaacctgc acgcaactgc tggtgcaggc
     7141 cctcgaccag ccgccacggc gctgcgccgt catcggcacg ctcggcagcg gtttccccgg
     7201 cgcgctcaac gcctcgatcc ataccacgcc cgatgccgtc acagtgcagc ggctcatggc
     7261 ggattttctc ggtcagggcg cggcgcacgt ggccatggag gtctcgtcgc acgcactcga
     7321 acaggggcgg gtgaatggcg tgctgtttca tgtcgcggta ttcaccaacc tgacgcgcga
     7381 ccatctcgat tatcacggcg acatgacggc ttatggcctg gccaaggcca gattattcgc
     7441 atacgaagga ttgaaatacg ccgtgatcaa ccacgacgac gatttcggac gccggttgct
     7501 ggccgacgtc ggcgcgccgg tgacttgttt gagctacggc atcgagggcg gcgacgtgcg
     7561 cgcgcgcgag gtgcggcctt cgcctgaagg actgtggctg cgggtcgaaa cgccttacgg
     7621 cgagaccgaa ttgcgcagtc cgctcatcgg gcactttaac gtatacaacc tgctggcggt
     7681 gttggcgacc ctgctggctc tgggagtgga gttgcgcgac gccgccgcgc gtctggcg
//
LOCUS       DBNGZE010000203         8953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_723913, whole genome shotgun sequence.
ACCESSION   DBNGZE010000203 DBNGZE010000000
VERSION     DBNGZE010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_723913"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..2499)
                     /gene="glgP"
                     /locus_tag="AC16FV_05300"
     CDS             complement(268..2499)
                     /gene="glgP"
                     /locus_tag="AC16FV_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR2849510.1"
                     /translation="MAYFCAEFGLHESFQIYSGGLGILAGDHCKAASDLNIPFVAVGM
                     LYRQGYFTQTIDARGSQVAHYHPTHFTDLPVDATLDAEGKEIHVQVDLPGKRVILKVW
                     KAKAGHITLYLLDSDLPENDEADRRITYQLYGGDINTRMQQEIVLGIGGVRALAAVGL
                     KPTVWHINEGHAAFLILERCREFVAQGLDFDSALELVAANTVFTTHTPVPAGHDIFDR
                     ALMDRYFGDKLKALGTSMEKLFSLGSSPGSQGGFNMTALALRGSRFRNGVSRIHGEVA
                     SRMAAYIWPQIPPEENPIGCITNGVHVPTFLAREWTNLFDMRFGGGWRNELLNEGFWK
                     HIDDIPDHSFWSLHQSLKAEMLAEVKRRVISQNRRNGTSEALIERYVQYLTPHDADVL
                     TVGFARRFATYKRATLLFSDPARLARLLNDPKRRMLLIFAGKAHPHDLPAQRLIEVIH
                     EYSRRPEFEGKLILLEGYDLALARKLVTSVDVWLNTPEYPLEASGTSGEKAGINGVIN
                     LSVLDGWWGEGYNGENGWAITPHGPQYDSTYRDRVEAQELLDIMEKQVIPLYYHRNGH
                     GYSEQWVKKSKISMKSLIPRFNAQRMVMDYVRNYYAPAKRQRLMMLGNNFARAREIAA
                     WRRRLDQSWPKVSARRVDDGRAEIRAGEALALRVKARLNGLHPDDVLVECLVGYEAEN
                     GEFIKLDSHIFAAETTEESGETQFKLDLYPRLPGLQYYKIRLFPFHPALSHRFESGYM
                     IWL"
     gene            complement(2604..2825)
                     /locus_tag="AC16FV_05305"
                     /pseudo
     CDS             complement(2604..2825)
                     /locus_tag="AC16FV_05305"
                     /inference="COORDINATES: protein motif:HMM:NF023325.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
     gene            2937..3974
                     /gene="queA"
                     /locus_tag="AC16FV_05310"
     CDS             2937..3974
                     /gene="queA"
                     /locus_tag="AC16FV_05310"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR2849511.1"
                     /translation="MRKSDFNYSLPPELVAQYPAPARTASRLLHLDGASGEYRDMKFA
                     DLPALLRAGDLLVFNDTRVIPARLFGHKDSGGRIEVLIERVLDAHRVLAQVRASKPPK
                     PGQELILEEDVTVTVRSRDGEFYELAFDTDKPVLDVLDNIGHVPLPPYIGRTDETADR
                     ERYQTVYARHRGAVAAPTAGLHFDEAMLGRLEDMGVDSAFVTLHVGAGTFQPLRVDDI
                     REHRMHAEYLRVTPEVCDKINAAKREGRRVVAVGTTVVRALETAAKDGRVHAFDSDTD
                     IFIYPGYRFQIVGALLTNFHLPESTLLMLVCAFGGTENVLNAYRHAVEQKYRFYSYGD
                     AMFVSLPPWAS"
     gene            complement(3929..5260)
                     /locus_tag="AC16FV_05315"
     CDS             complement(3929..5260)
                     /locus_tag="AC16FV_05315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2849512.1"
                     /translation="MLASPQLAGANNTIVRMVTNVGSFNVELYDTEAPITVANFLQYL
                     NGGFYNGTVFHRSIPGFALQGGGYVFVDFFGTRYFYEFDKFPPIQNEFSPSRSNIRGT
                     LAMAKLGGDPNSATSEWFINLADNGGSPDTDPPGLDYQNGGFTVFGRVLEPGMKVVDT
                     IADPDKVIATNVQIMGGAPRFFPALPVVDKIDKYPVVFNKICINNDSDGACPEIEDLA
                     PRGDGNGDGIPDRDQANVTTVQTLLGATATFEAEPMMRLDPVNAVNTPTATSLLTMFK
                     SPSDQSVHFNNGMYTFSITGVIDPAGNIVTMYDGASARPTHYYAFGPTSDNLVPHWYD
                     FAFDGETGAEIKSDSIMLHFVDGKRGDYDLTVNGNIDHIGAQAVVTAVDNGSASGGGC
                     SIAGTPPDVTRGGDWIVVSLFLAALVVVRRRARSGRRQDAQGGKDTNIASP"
     gene            5578..6723
                     /gene="tgt"
                     /locus_tag="AC16FV_05320"
     CDS             5578..6723
                     /gene="tgt"
                     /locus_tag="AC16FV_05320"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR2849513.1"
                     /translation="MSLIYESLGTDGLARRGRMVFERGTVETPAFMPVGTYGTVKGMT
                     PEELRETGAEIILGNTFHLMLRPGVEAIKAHGGLHRFMHWDGPILTDSGGFQVWSLGQ
                     LRKLSEKGVWFQSPVDGAKIFLGPEESMAVQQALDADIIMIFDECTHHPATEPEARAS
                     MELSLRWAERSKTAHADHPAALFGIVQGGMYEHLRDISLTGLKQIGFDGYAIGGLSVG
                     ETKEEMFRILDHLAPQMPADKPRYLMGVGMPEDLVEAVRRGMDMFDCVLPTRNARNGW
                     LFTHDGAVKIKNSCYARDTGPVDPLCDCYTCRHYSRAYLRHLQQANEILGARLATIHN
                     LHYYQRLMRGLRQAIGEKKLDDFIEKFYRMRRPAASPEALEDSTDAN"
     gene            6735..7079
                     /gene="yajC"
                     /locus_tag="AC16FV_05325"
     CDS             6735..7079
                     /gene="yajC"
                     /locus_tag="AC16FV_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR2849514.1"
                     /translation="MNFLISDAWAEAPAAPAGAPAQGSPFMTLIMLGVLFAAFYFILI
                     RPQAKRAKEHKAMITALAKGDEVIAAGGVLGKVTNLSDGYVTVDIAEGVEIKVQRQAV
                     QTVLPKGTIKSV"
     gene            7176..>8953
                     /gene="secD"
                     /locus_tag="AC16FV_05330"
     CDS             7176..>8953
                     /gene="secD"
                     /locus_tag="AC16FV_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR2849515.1"
                     /translation="MNKYPLWKYLLILAVVVIGFVYALPNIYGEYPAVQISPTRTTKV
                     DQALLTLAESQLKQANISYTSATLDEQGARIRFVDTETQIRARELIQTALGENYVVAL
                     NLLPATPAWLAAINGRPMYLGLDLRGGVHFLMQVDTAAVLKKAEENYTDDMRRVMREK
                     KVQYLTVNRLERGGIEIRFRDKAEREKGLELIRKELPDLEFTDIERSGEFIIQAKMKQ
                     TAVVEKSRFALEQNMNSLRNRVNELGVTEPIIQQQGQERIVVQLPGVQDTAKAKEILG
                     RTATLEIMMVDEEHDVAGALSGQVPAGSKLYKMRDGRPILLKNRLIYSGDNIIDSAPG
                     FESQSNTPIVSITLDARGAAINQRVTGENVGKRMAVIYIEIKSEPKLDEQGRPVLDAN
                     GRPLRVSKRVEEVITAPVIRSQLGKRFQIEGLDSVQEANELSLLLRAGAFAAPVEIIE
                     ERTVGPSLGADNIAKGFHSTWVGFAAISLFMIVYYVVFGFISVVALAVNVVLLVALLS
                     MLQATLTLPGMAGIALTVGMAIDANVLINERIREELRNGNTPQASIYAGYERAFGTIL
                     DSNVTNLIAGLALFMFGTGPIKGFAVV"
BASE COUNT         1765 a         2606 c         2760 g         1822 t
ORIGIN      
        1 atcccagtgg aaataacggc catgcatcca gcggtagccg cgcacgccca gcgcgatccc
       61 gaccatcgtg gccagcgccg gggtagcgcc ccttgatttt ggtcagtctt gcgctaccca
      121 tcgtcgaggg caccgaggcc tactcggggg atttcaacct ataggggcca tttcaaaccg
      181 cgcggcctgg ccagacatgt ggttcggtta tggaccgcac cggggcaggg cagcacggac
      241 ttacgccgcc tgcaagacca gggactgtta cagccagatc atgtaccccg actcgaagcg
      301 gtgcgagagc gcggggtgga acgggaacag gcggattttg tagtactgca gtcccgggag
      361 ccgcggataa aggtccagct tgaattgggt ctcgcccgat tcttccgtcg tctcggccgc
      421 gaagatgtgg ctgtctagct ttatgaattc gccgttctcc gcttcgtaac ccaccaggca
      481 ttcgaccagc acgtcgtccg gatgcaggcc attcaggcgc gccttcacgc gcagcgcgag
      541 cgcctcgccg gcgcggattt cagcccggcc gtcatcgacg cgcctggcgc tgactttcgg
      601 ccagctttga tcgaggcgcc tgcgccacgc cgcgatctcc ctggcgcgcg cgaagttgtt
      661 gcccagcatc atcagacgct ggcgcttggc gggcgcgtaa tagttgcgca cgtaatccat
      721 caccatgcgc tgggcattga agcgcgggat cagcgatttc atggaaatct tggatttttt
      781 cacccactgt tccgaatagc cgtggccgtt gcggtggtaa taaagcggaa tgacctgctt
      841 ttccatgatg tcgagcagtt cctgggcctc cacgcggtcg cggtaggtgc tgtcgtactg
      901 cgggccgtgc ggcgtgatgg cccagccgtt ctcgccgttg tagccctcgc cccaccagcc
      961 gtcgagcacg ctcaggttga tgacgccgtt gatgccggcc ttctctccgg aggtgccact
     1021 cgcttccagc ggatactccg gcgtgttgag ccagacgtcg acgctggtga cgagcttgcg
     1081 cgcgagcgcg agatcgtatc cctcgagcag gatgagcttg ccctcgaatt ccggacggcg
     1141 cgagtattcg tgaatgacct cgatcagccg ttgcgccggc aggtcgtgtg ggtgcgcctt
     1201 gccggcgaat atcagcagca tgcggcgttt cggatcgttg agcagccgcg ccaggcgcgc
     1261 cggatcggag aacagcagcg tggcgcgctt gtaggtggcg aagcgtcgcg cgaaacccac
     1321 cgtgagcacg tccgcatcgt gcggcgtgag gtattgtacg taacgctcga tcagggcctc
     1381 gctggtgccg ttgcggcgat tctgggaaat cacccggcgc ttgacctcgg ccagcatttc
     1441 cgccttgagc gactggtgca ggctccagaa actgtgatcg ggaatgtcgt cgatgtgctt
     1501 ccagaatccc tcgttgagca gctcgttgcg ccagccgccg ccgaagcgca tgtcgaagag
     1561 attggtccat tcgcgcgcca gaaacgtggg cacgtgcacg ccattggtga tgcagccgat
     1621 cggattctcc tccggcggga tctgcggcca gatgtaggcc gccatgcgcg aggcgacttc
     1681 gccgtgaatg cgggaaacgc cgttgcgaaa gcgcgagccg cgcagcgcca gcgccgtcat
     1741 gttgaagccg ccctggctgc cggggctcga tccgagggaa aacagttttt ccatgctcgt
     1801 gcccagcgcc ttcaatttgt cgccgaaata ccggtccatc agcgcgcggt cgaagatgtc
     1861 atggccggcc ggcacgggcg tgtgggtggt gaaaacggtg ttggccgcga ccagttccag
     1921 cgcgctgtcg aagtccagac cctgggcgac gaattcgcgg cagcgttcga gaatcaggaa
     1981 cgcggcgtgg ccttcgttga tatgccagac cgtcggtttg agcccgaccg cggccagcgc
     2041 gcgcacgccg ccgatgccga gcacgatttc ctgctgcatg cgcgtattga tgtcgccgcc
     2101 gtatagttga taggtgatgc gccggtcagc ctcgtcgttt tccggcagat cgctgtcgag
     2161 caggtagagc gtgatatggc cggccttggc cttccagacc ttgaggatga cacgcttgcc
     2221 cggcaggtcg acctgcacgt ggatttcctt gccttccgcg tccagtgtcg cgtccaccgg
     2281 caggtcggtg aagtgcgtgg ggtggtaatg cgccacctgg ctgccgcgcg cgtcgatggt
     2341 ctgggtgaaa tagccctggc ggtaaagcat gccgacggcg acgaagggga tgttgagatc
     2401 gctggccgct ttgcagtggt cgccggcgag aatgccgaga ccgccggagt agatctggaa
     2461 actttcatgc aggccgaatt cggcgcagaa gtaggccacc aggtcttcct cgggtcgagg
     2521 tgttgcagga tgtcggcgcg cgcggtttcc tgatggtagg tgtcgtagac cgacagcacg
     2581 cggttgtagt cctccatgaa cacgcggtcc tcggcggctt cgttcagccg cttctgcgcc
     2641 acgcgccgca gaaacacctt gggattgtgt ccgcaggatt cccacagttc gcgatcgagg
     2701 cggaagaaca gcgcgcgcac ctcgcggtcc cagctataga gcagatcgtt ggccaattcc
     2761 ttcaggcgtt tcaggcgatc cggaaggacc ggttggactt caagtgtgta gcgtgtgccg
     2821 ggcatatatt ctcgggttcg ttcggagaca atctcaggct acatagtata gtatgaccgc
     2881 ccgttcaggg tgactgcgtc ggttccatgc gcccaatgcg gcgtcccgtc aacatcatgc
     2941 gtaaatccga ttttaattat tccctgcctc ccgaattggt ggcgcaatat ccggcgccgg
     3001 cgcgtacggc gagccgtttg ctgcaccttg atggtgcaag cggcgaatac cgcgatatga
     3061 aatttgccga tttacccgcg ctgttgcgcg ccggtgattt gctcgtattc aatgacacgc
     3121 gcgtgattcc ggcgcgtttg ttcggacaca aggacagcgg tggacgcatc gaggtattga
     3181 tcgaacgcgt actcgatgca caccgcgtgc tcgcccaggt gcgggcgagc aaaccgccca
     3241 agccagggca ggaactaata ctggaagagg atgtaacggt gacggtgcga agccgtgatg
     3301 gcgagttcta tgaacttgcg tttgatacgg ataagccggt tttggacgtt ctcgataaca
     3361 tcggccatgt gcccttgccg ccgtacattg gtcgcacgga cgaaaccgcc gaccgggaac
     3421 gctaccaaac ggtgtacgcg cgtcatcgtg gtgccgtcgc ggcgcccacg gccgggctgc
     3481 atttcgacga ggccatgctc ggccggctgg aggacatggg cgtggacagc gctttcgtca
     3541 cgctgcacgt cggcgccggc acgtttcaac cattgcgggt ggacgatatc cgcgagcatc
     3601 gcatgcatgc ggaatatctc cgtgtcacgc cggaggtatg cgataaaatt aatgctgcga
     3661 aaagagaagg gcggcgcgtg gtggcggtcg gcactaccgt ggtcagggcg ctggagactg
     3721 ccgcgaagga tgggcgtgtg catgcctttg acagcgatac cgatatcttt atttaccccg
     3781 gttatcggtt ccagatcgtg ggcgcgctgt tgaccaactt ccatctgccg gaatccacgc
     3841 tgctcatgct ggtgtgcgcc ttcggcggaa cggaaaacgt gctcaacgcc taccgccacg
     3901 cggttgaaca gaaataccgg ttttacagct atggcgatgc gatgtttgta tccttgccgc
     3961 cttgggcatc ttgacgccgt ccgctgcggg cgcgccttcg tacgaccacg agcgcggcca
     4021 agaacagaga taccacgatc cagtcaccgc cacgcgtcac gtccggaggc gttccggcaa
     4081 ttgaacaacc cccgcctgag gccgagccgt tatccaccgc cgttaccacg gcctgcgcgc
     4141 ctatgtggtc aatgttgcca ttcacagtca ggtcgtaatc gccacgttta ccatcgacga
     4201 aatgcagcat gatactgtcg ctctttatct cggcgcctgt ttctccatcg aacgcaaaat
     4261 cgtaccagtg agggacgaga ttatccgatg tcgggccgaa ggcgtagtag tgggtaggac
     4321 gtgctgaggc gccgtcatac attgtgacaa tgttacctgc tggatcgatt acaccggtta
     4381 tcgaaaaggt gtacatgccg ttgttgaaat gcacggattg gtccgacggc gatttgaaca
     4441 tcgtcagcag cgaagtggct gtgggcgtgt taacggcatt aaccggatcc agccgcatca
     4501 ttggctccgc ctcgaatgta gccgtggcgc ccaacagcgt ctgtaccgtc gtaacgttag
     4561 cctgatcgcg gtccggtatt ccgtccccgt taccatcccc ccttggcgcc agatcctcga
     4621 tttccgggca cgcaccgtca ctgtcgttgt tgatgcagat tttgttgaag accaccgggt
     4681 atttatcgat cttgtccacg accggcagcg cgggaaaaaa tcttggagca ccacccatta
     4741 tttggacatt tgttgctatc actttatccg ggtcggcgat ggtatccacg accttcatcc
     4801 cgggctcgag aacacggcca aaaacagtga atcccccatt ctggtaatcg agaccgggag
     4861 ggtctgtgtc gggcgagccg ccgttgtcgg ccagattgat gaaccattca ctggtcgcgc
     4921 tgttcgggtc gccgcccaac ttggccatgg cgagtgtgcc acggatattc gatcgagaag
     4981 gagaaaactc gttctgaatc ggcggaaatt tatcaaattc gtaaaagtat cgcgtgccga
     5041 aaaaatcgac aaagacgtat ccgcctccct gcaaggcgaa gcccggaatg ctacggtgaa
     5101 aaactgtccc attatagaac ccaccattga ggtactgaag aaagttggca acggtgattg
     5161 gggcttctgt gtcgtaaagt tcgacgttga acgacccgac attagttacc atccgcacta
     5221 tcgtattgtt tgcgcctgcg agctggggtg aggcgagcat gacaaccaga aacgtacaga
     5281 cagtggcacg aaaaaatgcc attatttttc gacaagggtg acggaaaaaa accactcggg
     5341 aaattgcctg cgtaagaatc actaccctcc tcccgctccc tgtacattta ggagctttat
     5401 ttgtaccgcg cattgtcaca tagcgcgtgg aaggttccaa attcggaagt aacaggctgt
     5461 tttggtgtgc ttttacataa gtatgtagac gccctcagga ctgttattac cacggcggca
     5521 ttgatcgcac cgatgcaata agggtgtgga aaggctgtgc taccatcccc gcccgacatg
     5581 agcttgatat atgaatcgct tggcaccgac gggttagccc gccgcggacg catggtattt
     5641 gagcgcggga cggtcgagac gccggccttc atgccggtcg gcacctacgg cacggtcaag
     5701 ggcatgacgc cggaggaact gcgcgagacc ggcgccgaga tcattctggg caataccttt
     5761 catctcatgc tgcggccggg cgtggaggcg atcaaggcgc acggtggcct gcaccgtttc
     5821 atgcactggg acggtcccat cctcaccgac tcgggcggtt tccaggtgtg gagcttgggg
     5881 cagttgcgca agctgagcga gaagggcgtg tggttccagt cgccggtgga tggcgcgaag
     5941 attttcctcg ggccggagga gtccatggcg gtgcagcagg cgctcgacgc cgacattatt
     6001 atgatattcg acgagtgcac gcatcatcca gcgaccgagc ccgaggcgcg cgcctcgatg
     6061 gagctgtcgc tgcgttgggc cgagcgctcg aaaaccgcgc acgccgatca tccggccgcg
     6121 ctgttcggca tcgtgcaggg cggcatgtac gagcacctgc gcgatatttc gctcaccgga
     6181 ctcaaacaga tcggttttga cggttacgcc atcggcggcc tgtcggtggg cgaaaccaag
     6241 gaagaaatgt tccgcattct cgaccatctc gcgccgcaga tgccggccga caaaccgcgt
     6301 tatctcatgg gcgtgggcat gccggaagac ctcgtcgagg ccgtgcgtcg cggcatggac
     6361 atgttcgatt gcgtgctacc cacgcgcaac gcgcgcaatg gctggttgtt cacccacgat
     6421 ggcgcggtca agatcaaaaa cagctgttat gcccgggaca ccgggccggt ggatccgctg
     6481 tgcgactgtt atacctgccg ccattacagc cgcgcctatc tgcgccactt gcagcaggcc
     6541 aacgaaatcc tgggcgcccg gctcgccacc atccacaatc tgcactatta ccagcgcctg
     6601 atgcggggat tgcgtcaggc catcggtgaa aaaaaactgg atgatttcat cgagaagttt
     6661 tatcgtatgc gccgcccggc agcctccccg gaggcgctgg aagactcaac cgacgcaaac
     6721 tgaaggaaaa accgatgaat tttctgattt ccgacgcctg ggccgaggcc ccggccgctc
     6781 ccgcgggcgc accggcgcag ggcagccctt tcatgacatt gatcatgctc ggcgtgctgt
     6841 tcgccgcgtt ctatttcatc ctcatccgtc cgcaggccaa gcgcgccaag gaacacaagg
     6901 ccatgatcac cgcgctcgcc aagggcgatg aggtcatcgc cgccggcggc gtgctcggca
     6961 aggtcacgaa tctcagcgac ggctatgtca cggtggacat cgccgagggc gtggagatca
     7021 aggtacagcg tcaggcggtg cagaccgtcc tccccaaggg cacgatcaag tcggtttaag
     7081 aaatttaaac cgccaaggcg ccaagatcgc caagaaacaa gagttaatgt gattttttct
     7141 ggcgcccttg gcgtcttggc ggttcaagat caaaaatgaa taaatacccg ctctggaaat
     7201 acctgctgat tcttgccgtc gtcgtgatcg gctttgttta cgccttgccg aacatctacg
     7261 gcgaataccc ggcggtccag atatccccca cgcgcaccac caaggttgac caggcgctgc
     7321 tcacgctggc cgagagccag ttgaagcagg cgaatatttc ctataccagc gcgacattgg
     7381 atgagcaggg cgcgcgcatc cgtttcgtgg acacggaaac ccagatacgc gcgcgcgaac
     7441 tgatccagac tgcgctgggt gaaaactatg tcgtcgcgct caacctgctg ccagcgacgc
     7501 cggcctggct ggcggcgatc aacggcaggc cgatgtacct cggcctcgac ctgcgtggcg
     7561 gcgtgcattt cctgatgcag gtggacacgg cggcggtgct caaaaaggcc gaggagaatt
     7621 acaccgatga catgcgccgt gtcatgcgcg agaagaaggt gcagtatctg acggtgaacc
     7681 ggttggaacg tggcggcatc gaaatccgtt tccgcgacaa ggccgaacgc gaaaaaggcc
     7741 tggagctcat ccgcaaggag ctgcccgatc ttgaattcac cgacatcgaa cgcagcggcg
     7801 agttcatcat ccaggccaaa atgaagcaga cggcggtcgt ggaaaaaagc cggttcgcgc
     7861 tggaacaaaa catgaattcc ttgcgtaatc gcgtcaacga gctgggcgtg accgagccga
     7921 tcattcagca acaggggcag gagcgcatcg tggtgcagtt gcccggcgtg caggacaccg
     7981 ccaaggccaa ggagattctg ggacgtaccg cgacccttga aatcatgatg gtggatgagg
     8041 agcatgacgt ggcgggcgcc ctgtccggtc aggtgccggc cggatcgaag ttgtacaaga
     8101 tgcgcgatgg acgtccgatc ctgctcaaga atcgtctgat ctattcgggc gacaacatca
     8161 ttgattccgc ccccggcttc gagagccaga gcaacacccc gatcgtttcc atcacgctcg
     8221 atgcgcgcgg cgcggccatt aaccagcgtg tcaccggcga gaacgtcggc aagcgcatgg
     8281 cggtgattta catcgaaatc aagtccgagc ccaaactcga cgagcagggc aggccggtgc
     8341 tggatgcgaa cggacgtccg ctgcgcgtga gcaagcgcgt ggaagaggtg attaccgcgc
     8401 cggtgatccg ctcgcaactc ggcaagcgct ttcagatcga aggcctggac tcggtgcagg
     8461 aagcgaacga gctgtcgctg ctgttgcgtg ccggcgcttt cgccgcgccg gttgaaatta
     8521 tcgaggaacg caccgtcggc ccgagtctcg gcgctgacaa cattgccaag ggtttccact
     8581 cgacttgggt cggtttcgcg gcgatttcgc tgtttatgat cgtttattat gttgtctttg
     8641 gctttatctc ggtggtggcg ctggccgtca acgtggtgtt gctggtggcc ctgttgtcca
     8701 tgctccaggc cacgctgacc ctgcccggca tggccggtat cgcgctcacc gtcggtatgg
     8761 cgattgacgc caacgtgctg atcaacgagc gcattcgcga ggagttgcgc aacggcaata
     8821 cgccgcaggc cagcatctac gccggctatg agcgcgcctt cggcaccatc ctcgattcca
     8881 acgtgaccaa tctgatcgcg ggactggcgc tcttcatgtt cggtaccggc cctataaaag
     8941 gatttgccgt cgt
//
LOCUS       DBNGZE010000204         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1904623, whole genome shotgun sequence.
ACCESSION   DBNGZE010000204 DBNGZE010000000
VERSION     DBNGZE010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1904623"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1590
                     /gene="rng"
                     /locus_tag="AC16FV_05335"
     CDS             124..1590
                     /gene="rng"
                     /locus_tag="AC16FV_05335"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HIR2849516.1"
                     /translation="MSEEILINVTPQETRVAVVENGVLQEVYIERARSRGIVGNIYKG
                     KVVRILPGMQAAFVDIGHERTAFLHASDIRSVSAAEIGAGPPPPIHELLQDGQEVIVQ
                     VLKDPIGTKGARLTMQITLPARYLVYLPYSRHIGISQKIGDEATRERLRALVKSAMPE
                     GAEEGGYILRTLAETAAEEEIETDIRYLRRVWAACQERICSAPVLSLIHEDLPLALRA
                     MRDLVRGNVEKIRIDSREIFIKARDFGIEFIPEVAERIEHYPGERPIFDLYSVEDEIQ
                     KALDRKVMLKSGGYLILEQTEAMTTIDVNTGAYVGKRNLEETLFKTNLEAAQTIARQL
                     RLRNLGDMIIIDFIDMAEPEHKRQVLRALERAIARDRTKVYMTEMSPLGLVELTRKRT
                     RESLEHVLCQPCPVCEGRGMVKTPQTVCYEIFREILREARQYGADGYLVLASQPVIDL
                     LFDEEAGGMAKLQEFIGKPIQLKVEPLYTQEQYDVVLM"
     gene            1751..4126
                     /locus_tag="AC16FV_05340"
     CDS             1751..4126
                     /locus_tag="AC16FV_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR2849517.1"
                     /translation="MKKRLRQYAFHVGRVTLHVSRWTLYISAVVLVLLTITFGIARYL
                     LPMITEQKTNLEQYLSQRSGHQVRIDSLHAYWDGLHPGARVRGLQVFAADGIRPAIRL
                     SEVRISLALLPLLWGNFEINSLVVVNPSLALERLTDGRFRISGFDPLAPVERGQDEKF
                     VNWLFQQGRLEIENGELQWFDHRETGAALHLGRANLSLRNNGDRHRLTFNADFPDGMC
                     DECSLVLDVTGNPLASSDWDGDIYVRAGHVNSSALPLIAREKLPDNFRGKFALQLQSD
                     WEEGRPVSVRGYVKVADLRLPIRGWDTPLGIREASGDLVWRTRRAGWRLDMANPMLGL
                     NGPPWAAGHLRIEYQPDESEIQIKHVDLADVTGFVTRLKGEITESAKSAPGNAGALLD
                     AWLAAKPGGAVNNFSLRLAGDWMAPENYSLEADIKDGAALAWRKYPGVQGLGGHLSLS
                     RETGNFRIDSGNVKLSLPWVFRNPLAAQRVSGDLSWEKYADYWQVNGSDLRVTAEDGR
                     GTGKLSLQLPHDHAVSPVLKLRVDFQGGNGAHAARYYPASHLTPGTLAWMERSFVAGD
                     ITEGYLIYDGPIRDFPFHNRTGKFELRGHVRNGVYLFLPGWEPVKQAEVDVVIDGSEV
                     TVIGSGKIGKLDASQVVVQSRDSGMGHHVVHVGGKVSGALNETLNVLRDVKPEPGSAR
                     WLAYVPGGMQGSGTGILSLDLTIPLSDARATHVEGEYRFMKNTLRFAGSPVAVEGVEG
                     SVRFTEAGLHEGNLRARFLGGETVLAAAQREGRLVVRGQGAVMAQGLTPIV"
BASE COUNT          794 a         1205 c         1356 g          781 t
ORIGIN      
        1 aacaaaatga atatgatttt gaaccgccaa ggcgccaagg ccgccaagaa aggcgaattt
       61 caaattcata aaaacttggc gttcttggcg tcttggcggt taattcttat taattagaat
      121 ggcatgagcg aagagatact tatcaacgtt accccgcagg agacccgcgt cgccgtcgtg
      181 gaaaacggcg tgctgcagga ggtgtacatc gagcgcgcgc gcagccgcgg gatcgtcggc
      241 aatatatata aaggcaaggt agtccggatt ctgccgggca tgcaggcagc gttcgtcgac
      301 atcgggcatg agcgcacggc gtttctccac gcttcggaca tccgctctgt gtccgccgcc
      361 gagatcggcg ccggtcctcc gccgcccatc catgagttgt tgcaggacgg gcaggaggtg
      421 atcgtgcagg tgctgaagga ccccatcggc accaagggtg cgcgcctgac catgcagatc
      481 acgctgccgg cgcgatatct ggtttatctt ccctatagca ggcacatcgg catttcgcag
      541 aagatcggcg acgaggccac gcgcgagcgc ctgcgggcgc tggtgaaatc cgcgatgccg
      601 gaaggcgccg aggaaggtgg gtatatcctg cgcacgctgg cggagacggc ggcggaggag
      661 gagatcgaaa ccgacatccg ttatctgcgg cgggtatggg ccgcctgcca ggagcgcatc
      721 tgcagcgcgc ccgtgctcag cctgattcac gaggacctgc cgctggcgct gcgcgccatg
      781 cgcgatctgg tgcgcggcaa cgtcgaaaaa atccgcatcg actcgcgcga gattttcatc
      841 aaggcacgcg atttcggcat cgaattcatc cccgaggtgg ccgaacgcat agaacattac
      901 cccggcgaac gacccatatt cgatctgtac tcggtcgagg acgaaatcca gaaggccctc
      961 gaccgcaaag tcatgctcaa gtccggcggt tacctgatcc tggagcagac cgaggccatg
     1021 accacgatcg acgtgaacac cggcgcctat gtcggaaagc gcaacctgga agagacgctt
     1081 ttcaagacca acctggaagc ggcgcagacc atcgcgcgcc agctgcggtt gcgcaatctg
     1141 ggcgacatga tcattatcga cttcatcgac atggccgagc ccgagcacaa gcgtcaggtg
     1201 ctgcgcgcgc tcgagcgcgc catcgcgcgc gatcgcacca aggtctacat gacggagatg
     1261 tcgccgctgg ggctggtgga actcacgcgc aagcgcacgc gcgagagcct cgaacatgtc
     1321 ctgtgccagc cctgcccggt ctgcgaggga cgcggcatgg tgaagacgcc gcagaccgtc
     1381 tgttatgaga tcttccggga aatcctgcgc gaggcgcgcc agtacggcgc cgacggctat
     1441 ctggtgctgg cgtcgcagcc ggtgatcgat ctgttgttcg acgaggaggc cggtggcatg
     1501 gcgaagttgc aggagttcat cggcaagccg atccagctca aggtggaacc gctctatacg
     1561 caggaacaat acgatgtcgt gctgatgtga tgccggtctg tgtcggccgg cgcgcatgaa
     1621 cttacgtgga aatgcggtct caaaggaatc attccctgtc tcccgttgat atttttctgc
     1681 ttttggtccg gatgcggcag aatagcgcta tgaacttttc aggcaagccg gtgcaaggca
     1741 gtccattgac atgaagaaac gactgcgcca gtacgccttt catgtcggcc gcgtgacgct
     1801 gcacgtgagc cgctggaccc tgtatatctc ggcggtggtg ctggtgctgc tgacgatcac
     1861 tttcggcatc gcgcgctatc tgctgccgat gatcacggag cagaaaacga atctcgagca
     1921 gtatctgagc cagcgttccg gccatcaggt gcgcatcgat tcgctgcacg cctactggga
     1981 tggcctgcat cccggcgcgc gcgtccgggg attgcaggtg ttcgcggccg acggaatcag
     2041 gcccgccatc cggctgagcg aagtccgcat cagtctggcg ctgttgcccc tgctctgggg
     2101 taatttcgag atcaacagtc tggtggtggt gaatccgagc ctggcgctgg agcgcctcac
     2161 ggacggacgt ttccgtattt ccggattcga tccgctcgcg cctgtcgagc gcggtcagga
     2221 tgagaaattc gtcaactggc tgttccagca gggacggctg gagatcgaaa acggcgagct
     2281 gcagtggttc gatcaccgcg aaaccggcgc ggccctgcat ctcgggcgcg cgaacctgag
     2341 cctgcgcaac aacggcgacc ggcaccgcct cacattcaac gccgatttcc cggacggtat
     2401 gtgcgatgaa tgttcgctgg ttctcgatgt cacgggaaac ccgctggctt cgtctgattg
     2461 ggatggcgat atctatgtgc gcgccggaca cgtcaatagc agtgccctgc cgctgatcgc
     2521 gcgcgagaaa ctgccggaca attttcgcgg aaagtttgcc ctgcaactgc agtccgactg
     2581 ggaggagggc cgccctgttt ccgtcagggg ttatgtcaag gtcgccgacc tccggttgcc
     2641 gatacgggga tgggacacgc cgctcggaat acgcgaggcg agcggcgatc tggtctggcg
     2701 cacccgccgc gcgggctggc gtctcgacat ggccaatccc atgctgggcc tgaatggtcc
     2761 gccgtgggcc gccgggcatt tgcgcatcga atatcaaccg gatgaaagcg agatccagat
     2821 caagcacgtg gatctggccg acgtcaccgg ttttgtgacg cgcctgaaag gcgaaatcac
     2881 cgagagcgcg aaaagcgcgc cggggaacgc gggcgcgttg ctggatgcct ggctggcggc
     2941 caaacccggg ggcgctgtca ataatttcag tctgcgtctg gcgggcgact ggatggcgcc
     3001 cgagaactat tccctggaag cggatatcaa ggatggcgcc gcgctcgcgt ggcggaaata
     3061 tccgggcgtg cagggtctgg gtgggcatct gtccctgtcg cgcgagaccg gaaactttcg
     3121 gatcgattcc ggcaacgtga agctgtccct cccgtgggtg ttccgcaatc cgctggccgc
     3181 gcagcgcgtc agcggcgacc tgagctggga gaaatatgcc gattactggc aggtcaacgg
     3241 cagcgacctg cgcgtcaccg ccgaggatgg gcgtggcact ggcaagctca gcctgcaact
     3301 gccgcacgac catgccgtct cgccggtcct gaaactgcgc gttgattttc agggcggcaa
     3361 cggcgcgcac gccgcgcgtt actatccggc ctcgcacctg acgcccggca ccctcgcctg
     3421 gatggagcgt tcctttgtcg ccggcgatat caccgagggt tacctgatct atgacggacc
     3481 catacgtgat tttcccttcc acaaccgcac gggaaaattc gagctgcgcg gccatgtgcg
     3541 aaacggtgtt tacctttttc ttcccggctg ggagccggtc aagcaggctg aggtggacgt
     3601 ggtcatcgac ggttccgaag tgacggtgat cggcagcgga aaaatcggca agcttgatgc
     3661 gtcacaggtc gtggtgcagt cgcgcgattc cggcatggga caccatgtcg tgcatgtcgg
     3721 cggcaaggta agcggcgcgc tcaacgaaac gctgaatgtc ttgcgcgatg taaagcccga
     3781 gcccggcagc gcgcgctggc tggcctatgt gcccggcggc atgcagggtt cgggcacggg
     3841 cattctcagt ctggatctga cgattccgtt gagcgatgcg cgtgccaccc acgtcgaggg
     3901 cgagtaccga ttcatgaaaa acacgttgcg attcgccggt tcgccggtgg cggtggaagg
     3961 ggtcgagggc agcgtgcgtt tcacggaggc gggtttgcac gagggcaatc tgcgtgcgcg
     4021 ttttctcggt ggcgagaccg tgctggccgc ggcccagcgc gaaggccggc tggttgtgcg
     4081 cggacagggc gccgtcatgg cacagggact gacgccgatc gtttgagcga agattg
//
LOCUS       DBNGZE010000205         1746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_99123, whole genome shotgun sequence.
ACCESSION   DBNGZE010000205 DBNGZE010000000
VERSION     DBNGZE010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_99123"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16FV_05345"
     CDS             complement(<1..611)
                     /locus_tag="AC16FV_05345"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR2849518.1"
                     /translation="MAKKALITGITGQDGAYLAEFLLKKGYEVHGIKRRTSLFNTDRI
                     DHLYQDPHVKARHFILHYGDMTDSSSLIRIVQQVQPNEIYNLAAQSHVAVSFEEPEYT
                     ADSDALGALRLLEAIRILGLEKKTRFYQASTSELYGKVQEIPQRETTPFYPRSPYAVA
                     KLYAYWITVNYREAYRIYACNGILFNHESPVRGETFVTRKITRA"
     gene            complement(614..>1746)
                     /locus_tag="AC16FV_05350"
     CDS             complement(614..>1746)
                     /locus_tag="AC16FV_05350"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR2849519.1"
                     /translation="ALTLAALALLKHSSNDALMLVMPADHVIQNNEQFHAAVREGVAL
                     AEKGRLITFGVVPASPETGYGYIKRGDGNEVEAFVEKPDAKTAAQYVASGEYLWNSGM
                     FMMLASIWLDEIKRFHPAMLEACKAAYEGGRRDADFYRVDRQAFGSCQSNSIDYAVME
                     ETDRAAVVALDAGWSDIGAWSSLWDASARDAQGNVVQGDVYMHATQNALLISQHRFVA
                     AVGLDDIIVVETPDAVLVAHKKHAQDVKEVVNRLKSDKRSEYQTHRRVYRPWGSYEGI
                     DAGARFQVKRLMVNPGAALSLQMHHHRAEHWIVVKGTARVTRGDEIFMLTENQSTYIP
                     LGTTHRLENPGNIPLEIIEVQSGSYLGEDDIVRFEDKYNRTK"
BASE COUNT          336 a          531 c          491 g          388 t
ORIGIN      
        1 gcccgcgtaa tctttcgggt gacaaaggtc tcgccgcgca ccggagattc atggttgaag
       61 agaatcccgt tgcaggcgta tatgcggtag gcctcgcggt aattgaccgt gatccagtag
      121 gcatacagct tggccacggc atagggcgaa cgtggataaa acggcgtggt ctcgcgctgg
      181 gggatttcct gaaccttgcc gtatagctcg gatgtcgagg cctggtaaaa gcgggttttc
      241 ttttccaggc ccaggatacg gatggcctcc agcaggcgca acgcccccag ggcatccgaa
      301 tccgccgtgt attccggttc ctcgaatgac acggccacgt ggctctgggc cgccagattg
      361 tagatctcgt tcggctgcac ctgctgcacg atgcgaatca ggctgctgga atctgtcatg
      421 tcgccgtagt gaaggatgaa gtggcgcgcc ttcacatgcg gatcctgata caggtggtcg
      481 atgcgatcgg tgttgaacag cgaggtgcgg cgcttgatgc cgtggacttc atagcccttc
      541 ttgagcagga attcggccag ataggccccg tcctggccgg tgatgccggt aatcaatgct
      601 ttctttgcca ttttcatttt gtccggttgt acttgtcttc gaaacgcacg atgtcatcct
      661 caccgaggta gcttccggat tgcacctcga tgatctcgag cggtatgttt ccgggatttt
      721 ccagccggtg cgtcgtcccc aatgggatat aggtggactg gttttccgtg agcatgaata
      781 tctcgtcccc ccgcgtcacc cgggccgttc ccttgaccac gatccagtgc tcggcgcgat
      841 ggtgatgcat ctgcagcgac agcgccgcgc cgggattgac catcagccgc ttcacctgga
      901 agcgcgcacc ggcatcgata ccctcgtaag aaccccacgg acgatacacc cgtcggtggg
      961 tttgatattc gctgcgcttg tcgcttttca gacggttgac cacttccttc acgtcctgcg
     1021 cgtgtttctt gtgggccacc agcacggcat cgggtgtttc gaccacgata atatcgtcaa
     1081 gccccacggc ggcgacaaaa cggtgctgcg aaatcagcag cgcgttctgg gtcgcgtgca
     1141 tataaacatc cccctgcacg acgttcccct gtgcatcacg cgcggaggcg tcccacaggg
     1201 atgaccaagc accgatatcc gaccagcccg catccagcgc cacgactgcc gccctgtcgg
     1261 tttcctccat gacggcgtaa tcgatggaat tcgactggca actgccgaag gcctgccggt
     1321 caacccggta gaaatcggca tcgcgtcgcc ctccttcata ggcggccttg caagcctcca
     1381 gcatcgcggg atgaaaccgt tttatttcat cgagccagat tgaggcgagc atcatgaaca
     1441 tgccgctgtt ccacaaatac tcgcccgagg ccacatattg tgcggcggtt ttggcgtccg
     1501 gcttttccac gaacgcttcc acctcattgc cgtctccccg cttgatgtaa ccgtaaccgg
     1561 tttcaggtga cgcgggcaca accccgaagg taatcagccg acctttctcc gccagggcaa
     1621 ccccctccct gacagcggca tgaaactgtt cgttgttctg tataacatga tccgccggca
     1681 tcaccagcat caaggcgtca ttcgacgagt gcttcaagag cgccaaggca gcgagcgtca
     1741 gagccg
//
LOCUS       DBNGZE010000206         6503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_99170, whole genome shotgun sequence.
ACCESSION   DBNGZE010000206 DBNGZE010000000
VERSION     DBNGZE010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_99170"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="rplD"
                     /locus_tag="AC16FV_05355"
     CDS             <1..436
                     /gene="rplD"
                     /locus_tag="AC16FV_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR2849520.1"
                     /translation="PWRQKGTGQARAGTIRSPLWRGGGKIFPASPDENFEQKVNRKMY
                     RGALRSILSELLRQGRLVTVAEFKMDQPKTKALVDKLKKLETPDVLIVTDALDNNLSL
                     AARNLPGVDVRMVGAADPVSLIRHAKVILTQGAVKKFEEMLA"
     gene            433..726
                     /gene="rplW"
                     /locus_tag="AC16FV_05360"
     CDS             433..726
                     /gene="rplW"
                     /locus_tag="AC16FV_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR2849521.1"
                     /translation="MNDEKLFQVILSPHVSEKSSRLADKNRQMVFEVRPDATKPVIKQ
                     AVEKMFNVQVESVTVTNVQGKRKGGRNAGRRQDWKKAYVRLKPGQDINFVGQQ"
     gene            757..1578
                     /gene="rplB"
                     /locus_tag="AC16FV_05365"
     CDS             757..1578
                     /gene="rplB"
                     /locus_tag="AC16FV_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR2849522.1"
                     /translation="MALVKMKPTSAGSRAMVRVVKPDLHKGAPHTRLLEKKNRGSGRN
                     NYGRVTTRHQGGGHKQHYRMIDFKRDKDGVPGRIERLEYDPNRSANIALVLYADGDRR
                     YIIAPKGVEVGAEIQSGANAPIKPGNCLPLRSVPVGSTVHCVELKQRGGAQLGRAAGA
                     GIQLVAREGDYAQLRLRSGEVRKVHVDCRAVIGEVGNAEHSLRKLGKAGASRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRTKGGRHPVSPWGTPTKGYKTRANKRTNSMIVHRRK"
     gene            1596..1883
                     /gene="rpsS"
                     /locus_tag="AC16FV_05370"
     CDS             1596..1883
                     /gene="rpsS"
                     /locus_tag="AC16FV_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR2849523.1"
                     /translation="MPRSVRKGPYVDAHLATKVEKARMESSKKPIKTWSRRSTITPDF
                     VGLTVAVHNGRQHVPLLITENMVGHKLGEFAATRTFKAHSGNRKVTTEETT"
     gene            1888..2259
                     /gene="rplV"
                     /locus_tag="AC16FV_05375"
     CDS             1888..2259
                     /gene="rplV"
                     /locus_tag="AC16FV_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR2849524.1"
                     /translation="MQATAILKYVRVSAQKGRLVADQIRGMPVARALELLQFSPKKAA
                     GHVRKVLESAIANAEHNEGADVDELKVASIQVDGGPTLRRFHSRAKGRGVRILKRTSH
                     ITVVVSDEKKPSKTAVKKEKS"
     gene            2259..2942
                     /gene="rpsC"
                     /locus_tag="AC16FV_05380"
     CDS             2259..2942
                     /gene="rpsC"
                     /locus_tag="AC16FV_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR2849525.1"
                     /translation="MGQKVNPIGFRMGVSRDWTAKWYAGTKTYTQNLISDIALRDYLK
                     ARLKNAAVSTIVIERPAQSINITVHTARPGIVIGKKGEDIEKLRQELTKLAGRPVQLA
                     VEEVRQPELDAQLVAENIAQQLEKRIMFRRAMKRAVTNAMRLRALGIKIMVAGRLNGS
                     EIARTEWYREGRVPLHTLRADIDYGFSEAHTTYGKIGVKIWIFKGEVFDKDAPKMEDA
                     EAQKKGATA"
     gene            2960..3370
                     /gene="rplP"
                     /locus_tag="AC16FV_05385"
     CDS             2960..3370
                     /gene="rplP"
                     /locus_tag="AC16FV_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR2849526.1"
                     /translation="MLQPKRTKYRKQMKGRNRGLALRGNKVSFGEYGLKAITRGRLTS
                     RQIEAARRALTHFIKRGGRVWIRVFPDKPVSKKPLEVRMGSGKGNVEYWVAQVQPGAV
                     LYEMEGINEQEAREAFKLAAAKLPVRTTFVTRTL"
     gene            3382..3570
                     /gene="rpmC"
                     /locus_tag="AC16FV_05390"
     CDS             3382..3570
                     /gene="rpmC"
                     /locus_tag="AC16FV_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR2849527.1"
                     /translation="MNAKDYRAMDAAARQKEMTDMLREQFNLRMQKATGQLSNTANLT
                     KVRRNIARLRTVMNEKSG"
     gene            3574..3840
                     /gene="rpsQ"
                     /locus_tag="AC16FV_05395"
     CDS             3574..3840
                     /gene="rpsQ"
                     /locus_tag="AC16FV_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR2849528.1"
                     /translation="MTEQTKNARSATGRVVSNKMNKTITVQVDRRIRHPLYGKIITRR
                     SKLHAHDESNECKEGDLVVIEECRPLSKTKAWRLVKVLERAREV"
     gene            3929..4297
                     /gene="rplN"
                     /locus_tag="AC16FV_05400"
     CDS             3929..4297
                     /gene="rplN"
                     /locus_tag="AC16FV_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR2849529.1"
                     /translation="MIQMQTLLMVADNSGAKTVQCIKVLGGSHRRYAGIGDVIKVSVK
                     EAIPRGKVKKGDVYDAVIVRTRKGVRRTDGSVIRFDTNAAVLLDNKRELIGTRIFGPV
                     TRELRSEQFMKIISLAPEVL"
     gene            4311..4631
                     /gene="rplX"
                     /locus_tag="AC16FV_05405"
     CDS             4311..4631
                     /gene="rplX"
                     /locus_tag="AC16FV_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR2849530.1"
                     /translation="MKKIRKGDKVVVLAGRDKGKQGTVLRVFEDSRVLVESVNMVKRH
                     TKPNPNKGVTGGILDREAPIHVSNVALFNPATGKGERVGMRVMQDGSKVRFFKKSGEV
                     ADVK"
     gene            4643..5182
                     /gene="rplE"
                     /locus_tag="AC16FV_05410"
     CDS             4643..5182
                     /gene="rplE"
                     /locus_tag="AC16FV_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR2849531.1"
                     /translation="MARLKEHYRKNVIPALQAKFGYKNVMQVPRITKITLNMGVGEAM
                     ADKKVIDNAANDMTLISGQKPQITRARKSIANFKVRQNWPIGCKVTLRGARMYEFLDR
                     LISVAIPRIRDFRGLPSRSFDGRGNYSLGIKEQIIFPEIDFDKVDAIRGMDITIATSA
                     KTDAEARALLEGFNFPLRS"
     gene            5193..5498
                     /gene="rpsN"
                     /locus_tag="AC16FV_05415"
     CDS             5193..5498
                     /gene="rpsN"
                     /locus_tag="AC16FV_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR2849532.1"
                     /translation="MAKTSMINRDKNRTALVAKYAAKRAELRDKVKNLKLSDEERHAA
                     MIALQKLPRDSSYIRRRNRCSLTGRPRGYYRKFGLSRSKLREIMMRGEVPGVVKASW"
     gene            5513..5905
                     /gene="rpsH"
                     /locus_tag="AC16FV_05420"
     CDS             5513..5905
                     /gene="rpsH"
                     /locus_tag="AC16FV_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR2849533.1"
                     /translation="MMTDPIADLLTRIRNGQMAEKVSVRIPASRLKLAVAKVLQDEGY
                     IEACREVTEDGRPLIEVALKYHDGQPVIERLERVSTPGLRRYEGKHKLPRVEGGMGVA
                     IVSTSKGIMSDRAARKAGLGGEILCIVA"
     gene            5919..6452
                     /gene="rplF"
                     /locus_tag="AC16FV_05425"
     CDS             5919..6452
                     /gene="rplF"
                     /locus_tag="AC16FV_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR2849534.1"
                     /translation="MSRIAKNPVAVPSGVQVALTGAKLSVKGPKGNLEHEVHPLVKVT
                     QEGNLLKFQRTNDSTLARAVSGTTRALVNNMVNGVSKGFEKKLTIIGVGYRVAVQGKK
                     LNLTLGYSHPIVYDIPNGITIEAPDQTNLVIKGADRQLVGQVAAEIRDYRAPEPYKGK
                     GVRYVNEVVVKKEAKKK"
BASE COUNT         1504 a         1867 c         2103 g         1029 t
ORIGIN      
        1 gccgtggcgc cagaagggca ccggccaggc gcgcgcgggc acgatccgca gccctctgtg
       61 gcgcggcggc ggcaagattt tcccggcctc gccggatgaa aacttcgaac agaaggtcaa
      121 ccgcaaaatg tatcgcggcg cgctgcgctc gatcctgtcc gagctgctgc gacagggccg
      181 gctggtgacg gtggccgaat tcaagatgga tcagccgaaa accaaggcac tggtggacaa
      241 gctcaagaag ctcgagacgc cggacgtgct gatcgtgacc gatgcgctgg ataacaacct
      301 gtcgctggcg gcgcgcaatc tcccgggcgt ggacgtgcgc atggtcggcg cggccgatcc
      361 ggtgagcctg atccgccacg cgaaagtcat cctgacgcaa ggcgcggtca agaagttcga
      421 ggagatgctg gcatgaacga tgagaaactg tttcaggtga ttctctcgcc gcacgtctcc
      481 gagaaaagtt cacgcctggc ggacaagaac cggcagatgg tattcgaggt acgtcccgac
      541 gcgaccaagc ccgtgatcaa gcaggcggtg gaaaaaatgt tcaacgttca ggtggaatcg
      601 gtgacagtga cgaacgtgca gggcaagcgc aagggcggac gcaacgccgg ccgccggcag
      661 gactggaaaa aggcctatgt gcgtctgaag ccgggccagg acatcaattt cgtcggacag
      721 caatagcgaa agattaaaga ttaaggaaat caggcaatgg cactggtcaa gatgaaaccg
      781 acgtcggcag gttcgcgcgc catggtccgg gtggtaaagc ccgacctgca taaaggcgcg
      841 ccgcatacgc gcctgctcga aaagaaaaac cgcggcagcg gtcgtaacaa ctacggccgc
      901 gtcacgacgc gccaccaggg cggcggtcac aaacagcatt accgcatgat cgatttcaag
      961 cgcgacaagg acggtgtgcc gggtcgcatc gagcgcctgg aatacgatcc caaccgcagc
     1021 gccaacatcg cgctggtgtt gtatgccgac ggcgatcgtc gttacatcat cgcgcccaag
     1081 ggcgtggagg ttggcgccga aatccaatcg ggtgcgaatg ccccgatcaa gccgggcaac
     1141 tgcctgccgc tgcgcagcgt acccgttggc agcaccgtgc attgcgtcga gctcaagcaa
     1201 cgcggcgggg cccagctcgg acgcgccgcg ggcgccggca tacagctggt cgcgcgtgag
     1261 ggcgactacg cccagctgcg gctgcgttcc ggtgaagtac gcaaggtgca cgtcgactgc
     1321 cgcgccgtca ttggcgaggt cggcaacgcg gaacacagcc tgcgcaagct cggcaaggcg
     1381 ggcgcatcgc gctggcgcgg cattcgtccg acggtgcgcg gcaccgccat gaacccggtg
     1441 gatcatccgc acggtggcgg cgaaggccgc accaagggcg ggcgtcatcc ggtcagcccc
     1501 tggggtacgc cgaccaaggg ttacaagacg cgcgcgaaca agcgcaccaa ttccatgatt
     1561 gttcaccggc gcaagtagaa aaggggtttt gatccgtgcc acgttcagtt agaaaaggac
     1621 catacgttga tgcgcacctc gccacgaagg tggagaaggc gcgcatggaa agcagcaaga
     1681 agccgatcaa gacctggtcg cgccgctcga cgatcacgcc ggatttcgtc ggcctgacgg
     1741 tcgcggtgca caacggacgg cagcatgtgc cgctgctgat caccgagaac atggtcggcc
     1801 acaagctcgg cgaattcgcg gccacccgca cgttcaaggc gcactcgggc aaccgcaaag
     1861 tgacgacgga agagacgaca taacgccatg caagccactg ccattctgaa atatgtccgg
     1921 gtgtccgcgc agaagggacg cctcgtcgcc gaccagattc gcggtatgcc ggtcgcgcgc
     1981 gccctggaac tgcttcaatt cagccccaag aaggccgcag gacacgtgcg caaggtgctc
     2041 gaatccgcca tcgccaacgc cgaacacaac gaaggcgcgg acgtggacga actcaaggtg
     2101 gccagcatcc aggtggacgg cgggccgacg ctgcggcgct ttcattcgcg cgccaagggg
     2161 cgtggcgtgc gcattctcaa gcgcaccagc catatcaccg tggtggtgag cgacgagaaa
     2221 aaaccgtcca agacggcggt gaagaaggag aagagctgat gggccagaaa gtcaatccga
     2281 tcggattccg catgggggtc agccgtgact ggaccgccaa gtggtatgcc ggcaccaaga
     2341 cctacaccca gaatctgatc tcggacatcg cgctgcgcga ttacctcaag gcgcgactga
     2401 agaacgcggc ggtcagcacg atcgtgatcg agcgtccggc ccagagcatc aatattacgg
     2461 tgcacacggc gcgtcccggc atcgtcatcg gcaagaaggg cgaggacatc gagaagctgc
     2521 gccaggaact gaccaagctc gccggccgtc cggtgcagct ggcggtggaa gaagtgcgtc
     2581 agccggaact ggacgcgcag ctggtggccg agaacatcgc ccagcagctg gagaagcgca
     2641 tcatgttccg ccgcgccatg aagcgcgccg tgaccaatgc catgcgcctg cgcgcgctgg
     2701 gcatcaagat catggtcgcc gggcgactga acggttccga gatcgcgcgc accgagtggt
     2761 atcgcgaagg tcgcgtgccg ttgcatacgc tgcgcgccga catcgattac ggtttttccg
     2821 aggctcacac gacctacggc aagatcggcg tgaagatctg gatcttcaag ggcgaggtgt
     2881 tcgacaagga cgcgcccaag atggaagacg ccgaggccca gaagaaaggc gcgacggcgt
     2941 aacttaggaa ttcattacca tgttgcaacc gaaacgaacg aaatatcgca agcagatgaa
     3001 gggccgcaac cgcggcctgg cgctgcgcgg caacaaggtc agcttcggcg aatacggcct
     3061 gaaggccatc acgcgcggac gcctgacttc gcgccagatc gaggccgcgc gccgcgcgtt
     3121 gacgcacttc atcaagcgtg gcggacgcgt gtggatccgc gtgttcccgg acaagccggt
     3181 ttccaagaaa ccgctggaag tccgcatggg cagcggcaag ggtaacgtgg agtactgggt
     3241 ggcgcaggtt caaccgggcg ccgtgctgta tgaaatggaa ggcatcaacg agcaggaagc
     3301 ccgcgaggca ttcaagctcg ctgccgccaa gctcccggtc cgaaccacgt tcgtgaccag
     3361 aacactgtag gcagaatgac gatgaatgca aaagattatc gagcgatgga tgccgcggca
     3421 cgtcagaagg aaatgaccga catgctgcgc gagcagttca atctgcgcat gcagaaggcg
     3481 accggacagt tgtccaatac ggcgaatctg accaaggtgc ggcgcaacat cgcgcgcctg
     3541 cgcacggtca tgaacgagaa gtcaggataa ggcatgactg aacagacgaa aaatgcacgc
     3601 tcggccacgg gtcgcgtggt cagcaacaag atgaacaaga cgattaccgt gcaggtagat
     3661 cgtcgtatcc ggcatccgtt gtacggcaag atcatcacgc gccgctccaa gctgcatgcg
     3721 cacgatgaaa gcaacgagtg caaggaaggc gatctggtgg tgatcgagga atgccgtccg
     3781 ctttccaaga ccaaggcctg gcgcctggtc aaggtactgg agcgggcccg ggaagtttga
     3841 ataaggtctt gtttatggcc ggttttgagg caagtgttat aatcgcgcgt tttcgcccgg
     3901 ggcggagacg ccgtgtggag acaggacaat gatccagatg caaaccctgc tgatggtcgc
     3961 tgacaacagc ggggcaaaaa cggtgcagtg catcaaggtg ctgggcggct cccatcgccg
     4021 ctatgccggc attggcgacg tgatcaaggt cagcgtgaag gaagcgattc cgcgcggcaa
     4081 ggtcaagaag ggcgatgttt acgacgccgt catcgtgcgt acccgcaagg gcgtgcggcg
     4141 cacggacgga tcggtcatcc gcttcgacac caacgcggcg gtcttgctgg ataacaagcg
     4201 cgagctgatc ggcacccgta tcttcggacc cgtgacgcgt gagctgcgca gcgagcagtt
     4261 catgaagatc atctcgttgg cgccggaagt actgtaagag gcaggaagtc atgaaaaaga
     4321 ttcgaaaggg cgacaaggtg gtggtacttg ccggtcgcga caagggcaag cagggcacgg
     4381 tcctgcgcgt gttcgaggac agccgcgtgc tggtcgagag cgtgaacatg gtcaagcgtc
     4441 acaccaagcc gaaccccaac aagggggtga ccggcggtat tctcgaccgc gaggcgccga
     4501 ttcacgtctc caatgtcgcg ctgttcaacc cggccacggg caagggcgaa cgcgtgggca
     4561 tgcgcgtgat gcaggacggc agcaaggtca ggtttttcaa gaagagcggc gaagtcgccg
     4621 acgtgaaata aggtttcaga cgatggcaag gcttaaggaa cattaccgga aaaacgtgat
     4681 acccgcgttg caggccaagt tcggctacaa gaacgttatg caggtgccgc ggatcaccaa
     4741 gatcaccctc aacatgggtg tgggcgaggc catggctgac aagaaggtga tcgataacgc
     4801 cgccaatgac atgacgctga tcagcggcca gaagccgcag atcacgcgtg cgcgcaagtc
     4861 gattgccaac ttcaaggtgc gccagaactg gccgatcggt tgcaaggtga cgctgcgcgg
     4921 cgcgcgcatg tatgaatttc tggatcgcct gatcagcgtt gcgatcccgc gcatccgcga
     4981 tttccgcggc ctgccttcgc gttcgttcga cggacgcggc aattattcgc tcggcatcaa
     5041 ggaacagatt attttccccg agatcgattt cgacaaggtc gacgcgatcc gcggaatgga
     5101 catcaccatc gccaccagcg ccaagaccga tgcggaagca cgcgcgctgc tggaaggatt
     5161 caattttccg ctgcgcagct gaggattttt gcatggccaa gacaagcatg atcaatcgtg
     5221 acaagaaccg cacggcgctg gttgccaagt acgccgccaa gcgcgccgaa ctgcgcgaca
     5281 aggtgaagaa cctgaagctg tccgacgagg aacggcacgc cgccatgatc gccctgcaga
     5341 aactgccgcg cgattcgagc tacatccgcc gccgcaaccg ttgcagcctg accggccgtc
     5401 cgcgcggtta ttaccgcaaa ttcgggctgt cccgcagcaa gctgcgcgag atcatgatgc
     5461 gcggcgaagt gccgggcgtg gtcaaggcga gctggtagga gatactgaat atatgatgac
     5521 cgatccgatt gccgacctgc tgacccgcat ccgcaacggc cagatggccg agaaggtgag
     5581 cgtgcgtatt cccgcttcgc gcctcaagct tgcggttgcc aaggtgttgc aggacgaagg
     5641 ctatatcgaa gcctgccgtg aggtcacgga agacggccgg ccactgatcg aggtggccct
     5701 gaagtatcac gacgggcaac cggtgatcga gcgccttgag cgcgtgagca cacccggtct
     5761 gcgccgctac gaaggcaagc acaagctgcc gcgcgtcgaa ggcggcatgg gcgtggccat
     5821 cgtctccact tccaagggca tcatgtccga ccgcgccgcc cgcaaggccg gcctgggcgg
     5881 cgaaatcctg tgcatcgttg cgtaaagagt attgaaccat gtcacgaatc gcgaaaaatc
     5941 cggtggctgt cccgagcggc gtgcaagtgg cgctcacggg cgccaagctc agcgtcaagg
     6001 gccccaaggg caatctggag catgaggttc acccactggt aaaagtgacc caggagggaa
     6061 atctgctcaa gtttcagcgc accaacgatt cgacgctggc gcgcgctgtt tccggcacca
     6121 cgcgggcgct ggtgaataac atggtgaacg gcgtgtccaa gggcttcgag aaaaaactta
     6181 cgatcattgg cgtcggttat cgcgtcgcgg tgcagggcaa gaagctcaat ctgacgctgg
     6241 gttactcgca tccgatcgtg tacgacatcc ccaacgggat caccatcgaa gcgccggacc
     6301 agaccaatct cgtgatcaag ggtgcggacc ggcagttggt aggacaggtg gccgccgaga
     6361 tccgcgacta ccgcgcaccg gaaccgtata agggtaaggg cgtgcgctac gtcaatgaag
     6421 tcgtagttaa gaaagaagcc aagaagaagt aacgggcttc gagtcaggta aacgaatttt
     6481 atgaaaagag aaatcgatac acg
//
LOCUS       DBNGZE010000207         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1762669, whole genome shotgun sequence.
ACCESSION   DBNGZE010000207 DBNGZE010000000
VERSION     DBNGZE010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1762669"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /gene="ubiU"
                     /locus_tag="AC16FV_05430"
     CDS             complement(<1..907)
                     /gene="ubiU"
                     /locus_tag="AC16FV_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiU"
                     /protein_id="HIR2849535.1"
                     /translation="MELVCPAGSMPALKAAVDNGADAVYLGFRDGTNARNFPGLNFDL
                     DEARRAVHYAHQRGARVFLAINTYPRPSQWQKWCRAVDQAATLGIDALILADPGLMDY
                     TAQTYPDLRLHLSVQGSATSHEAIDFYVRQFGVRRVVLPRVLSLTQVQNVMKRVNAEV
                     EVFGFGSLCVMVEGRCTLSSYVTGESPNSSGVCSPAKHVKWEESSGGRRSRLNGVLID
                     VYAPEEPAGYPTLCKGRFEVGGNVYYAMEEPTSLNVLDILPELYRAGVAAIKVEGRQR
                     SPAYVAQVTRTLREAIDACAAAPETF"
     gene            complement(933..1568)
                     /locus_tag="AC16FV_05435"
     CDS             complement(933..1568)
                     /locus_tag="AC16FV_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HIR2849536.1"
                     /translation="MPETREAAQQAGPEQVVGYTLRDSRYLNITNRCTLRCAFCPKFN
                     DEWTVQDYRLRLEHEPTLEEIIAAVGNPEEYREIVFCGLGEPTLRLYTLLEAATRLRH
                     RAKRIRINTDGLANLVYGRDVTPDMEGLIDALSISLNAQNAEIYNLHCRPKLPGAYEA
                     ILDFVKHAREFVPEITLTAIDGLPGVDIAACERIARDLGVNFRRRVLNVVG"
     gene            complement(1693..>2004)
                     /locus_tag="AC16FV_05440"
     CDS             complement(1693..>2004)
                     /locus_tag="AC16FV_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849537.1"
                     /translation="QFLAGIQQMLEAAERNDAVAIAKIARPLGMAAAHAVPAGLAAKL
                     PLEFKTQGHGVHGDFDRIAMDAEAMNDVRLSLRQLGETMKKCVACHAAYQIATAPGPQ
                     R"
BASE COUNT          366 a          637 c          635 g          366 t
ORIGIN      
        1 caaatgtttc cggcgcggcg gcgcaggcat cgatggcctc gcgcagggtg cgcgtgacct
       61 gcgccacgta ggccgggctg cgctgccggc cctcgacctt gatcgccgcc acgccggcac
      121 gatacaactc cggcaggata tcgagcacgt tgagactggt cggctcctcc atggcgtaat
      181 aaacgttgcc gccgacttcg aaccgtccct tgcacagcgt cggatagcca gcgggttcct
      241 ccggcgcgta aacgtcgatc agcacgccgt tcagacgcga gcgccggccg ccggacgatt
      301 cctcccattt cacgtgcttc gcgggcgaac acacgccgct ggaattcggc gactcgccgg
      361 tgacgtagga cgacagcgtg caacggcctt caaccatgac gcacaggctg ccgaaaccga
      421 aaacctccac ctctgcattc acgcgcttca tgacattctg cacctgcgtg agcgacagca
      481 cgcgcggcaa caccacgcgc cgcacgccga actgccgcac gtaaaaatcg atcgcctcgt
      541 ggctggtggc cgaaccctgc accgagagat gcagacgcag atcggggtag gtctgcgcgg
      601 tgtaatccat gagtccgggg tcggcgagga tcagcgcgtc gataccgagg gtggccgcct
      661 ggtccacggc gcggcaccac ttctgccact gggatggacg cggataggta ttgatcgcca
      721 ggaaaacgcg cgcgccacgc tggtgcgcgt agtgcacggc ccggcgcgcc tcgtccagat
      781 cgaaattgag cccggggaaa ttgcgcgcgt tggtgccgtc acgaaagccg agataaacgg
      841 catcggcgcc gttgtccact gcggccttca gcgccggcat gctcccggcc ggacagacaa
      901 gctccatttt tccggcggct gacaaggatg aatcaaccta cgacgttgag tacgcgacga
      961 cggaaattca cgccaagatc gcgtgcgatt ctctcgcacg ccgcaatatc cacgccgggc
     1021 aatccgtcaa tcgcggtcaa ggtgatctcg ggcacgaact cgcgcgcgtg tttcacgaaa
     1081 tccagtatcg cctcataagc cccgggcagt ttcggccgac agtgcaggtt gtatatttcg
     1141 gcattctggg cattgagtga aatcgacagt gcgtcgatca ggccttccat gtcgggtgtc
     1201 acatcgcgcc cgtacacgag attggccagc ccatcggtgt tgatgcgtat gcgcttggcg
     1261 cggtggcgca ggcgcgtcgc cgcttccagc agcgtgtaca ggcgcagggt gggctcgccc
     1321 agcccgcaga acacgatctc gcggtattcc tccggattgc ccacggcggc gatgatttcc
     1381 tcgagcgtgg gttcatgctc gagacgcagc cgataatcct gcaccgtcca ctcgtcgttg
     1441 aatttcgggc agaacgcgca gcgcaatgtg cagcggttgg tgatgttcag gtaccggctg
     1501 tcacgcaggg tataaccgac tacttgctcc gggccggctt gttgcgccgc ttcccttgtt
     1561 tcaggcattg cacgtaccct ccgagaatgc ggaattttag gcacatcgcc gggatgcgcg
     1621 cttgatacag atcaaaggcg gtccgccgcg ggcgggcgga aaaaacaggt gaaaatcggc
     1681 gtgccgggga aactaccgct ggggtccagg tgctgttgcg atctggtagg cggcgtggca
     1741 agccacgcat ttcttcatgg tctcacccag ctggcgcagc gacagccgca cgtcgttcat
     1801 ggcctcggcg tccatggcga tgcggtcgaa atcgccgtgc acgccgtgcc cctgtgtctt
     1861 gaattccagc gggagtttcg cggccaggcc ggcgggaacg gcatgcgccg ccgccatccc
     1921 gagcggacgc gcgatcttcg cgatcgcgac ggcgtcgttg cgttcggccg cttccagcat
     1981 ttgctggatg ccggcgagga actg
//
LOCUS       DBNGZE010000208         3318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_307508, whole genome shotgun sequence.
ACCESSION   DBNGZE010000208 DBNGZE010000000
VERSION     DBNGZE010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_307508"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1652)
                     /locus_tag="AC16FV_05445"
     CDS             complement(429..1652)
                     /locus_tag="AC16FV_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HIR2849538.1"
                     /translation="MSRPVLEVADIFRGHGPAWRQANAGHVSLGQLKVMSAIENCRTA
                     ALGGHVARCENEKCAYTEIAYNSCRNRHCPKCQGTAAREWLAEREAELLPVPYFHVVF
                     SLPAQIADIAYQNKAVIYDILFKASAETMIAIAADPKHLGARIGVLSVLHTWGSALTH
                     HPHVHMIVPGGGISLDRKRWVACRPNFFLPVRVLSRLFRRLVLERLDAAHRAGQLQFF
                     GKHVALTNAPAFAAYLAPLHGSEWVVYSKRPFGGPEEVLRYLARYTHRVAISNRRLVA
                     LDDNGVTFKWKDYRIEGPDRYKLMTLDTHEFIRRFLMHVLPQGFHRIRYYGWLTSQTR
                     VKNIERIRELLAAPLIPIDAIKAVDAKPEEPKAPEHPCPCCGRRMRIIETFLRGQQPK
                     HCRSPVSPKVRIDTS"
     gene            complement(1665..2585)
                     /locus_tag="AC16FV_05450"
     CDS             complement(1665..2585)
                     /locus_tag="AC16FV_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015496924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR2849539.1"
                     /translation="MTTGTISPLRQRMIEDMNARKLCAGTQRGHISSCKRFAAFLKRS
                     PDTATLEDIRRFQLHLAETGASICNRNRIMTGLRFLFRVTLRRLDLAAEIYHLREPRK
                     IPLVMSTDETKRLLAVASSLKARLLLSLGYGCGLRAGEVVRLKVKHIDSAQKIIRIEQ
                     SKGRKDRNVMLSSETLDLLRQWWKVRPSRQDAQTPAPERWLFPGTRAGKPMTTRQLNR
                     LFHEAAEAAGISKGVTLHALRHSFATHLLERGTDIRIIQALLGHDKLDTTARYTRVAT
                     GMIAAIESPLDLLSLPRNKPRKNRKDPPPA"
     gene            complement(2896..>3318)
                     /locus_tag="AC16FV_05455"
     CDS             complement(2896..>3318)
                     /locus_tag="AC16FV_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR2849540.1"
                     /translation="TAPPGNWVESRFGGGSRSGLSGQFGVGLLLGAVWAPCAGPTLGA
                     ASVLAAQGRNLLQVALTMLFFAVGTALPLLALGFASREALMRWRTRMLSAGSTGRLIL
                     GSALTVTGVFILTGWDKMAATVLVGRMPTWMSELAASF"
BASE COUNT          606 a          986 c         1115 g          611 t
ORIGIN      
        1 gcgagcgctt cgcccaggtc tcctgcggca cgccctctac ctcacctcgc acttctgctt
       61 ctgtgcaggt tttcggatgc cgggatggcg ggactgtcca cgcgctccag cggccggcgt
      121 ccgaaaagcg ccaaggcacg aaatcgcggg atgtggacgg cagcggtgcc gtgggtgcta
      181 tggggatttg gccacgggct caggtgtgcc tgtgatggcg cgactcggca gcgagacccg
      241 ttttgcctgg taggcggaac ataggttgaa gcacgatcga gcaatgggcg ttttgagcga
      301 cgagattttg tgctttttgg ttgtgattgc gcaacaatcc ggcgtggcga ggcaagcggc
      361 ggcgctgtcg ggcaagagcc aacacaggcg gtgagtgttg ccatgtgtgt cgaggactgg
      421 cgtcggcctc atgaggtgtc gatcctgacc tttggcgaga ccggcgagcg gcagtgcttg
      481 ggctgttgcc cgcgcaagaa ggtctcgatg atgcgcatgc ggcggccgca gcaaggacaa
      541 ggatgctctg gcgcttttgg ctcttcgggc ttggcgtcga cagccttgat ggcatcgatc
      601 gggatgagtg gcgctgcaag cagttcgcgg atgcgttcga tattcttgac gcgggtttgg
      661 ctggtgagcc aaccgtaata acggatacgg tggaagcctt gcggcaagac atgcatcagg
      721 aagcggcgga tgaactcgtg ggtatcgagc gtcatcaact tgtatcggtc gggaccttcg
      781 atgcggtagt ccttccactt gaaggtgacg ccgttgtcgt cgagcgcgac caggcggcgg
      841 ttggagatgg cgacgcggtg ggtgtagcgc gcgagatagc gcaacacttc ctcggggcca
      901 ccgaacggcc gtttgctgta aacgacccat tcgctgccgt gcagtggagc cagataggcg
      961 gcaaaggctg gggcattggt cagggcaacg tgcttgccga agaactgtag ctgcccggcg
     1021 cggtgagcgg catcaagcct ctccaggacc agcctgcgga acaggcgcga gagcacgcgc
     1081 acgggcagga agaagttggg gcggcaggcc acccagcgct tgcgatcaag cgagatgccg
     1141 ccgcctggca cgatcatgtg gacatgcgga tggtgggtga gcgccgagcc ccaggtgtgg
     1201 aggacggaga ggacgccgat gcgggcgccc aggtgcttgg gatcggccgc gatggcgatc
     1261 atggtctcgg ccgaagcctt gaacaggatg tcgtagatca cggccttgtt ctggtaggca
     1321 atgtcggcga tttgggccgg cagcgagaac acgacgtgga agtaaggcac cggcaatagc
     1381 tcggcctcgc gctcggcgag ccattccctt gccgccgttc cctggcattt ggggcaatgc
     1441 cggttgcggc aggagttgta ggcgatctcc gtataggcgc acttctcgtt ctcgcagcgc
     1501 gcgacatgac cgccgagcgc tgccgtacgg cagttctcga tcgccgacat caccttgagt
     1561 tggccgaggc tcacatggcc ggcattggct tggcgccatg ccggtccatg gccgcggaag
     1621 atatccgcaa cctccaatac tgggcgggac atggccgcgg gcggttatgc cggcggcggg
     1681 tctttcctgt tcttcctggg cttgttgcga ggcaacgaca gcaggtcgag cgggctctcg
     1741 atggcagcga tcatgccggt ggcaacgcgc gtgtagcgcg ccgtggtgtc caatttgtcg
     1801 tgccccaaca acgcttggat gatgcggatg tcggttccgc gttccagcag gtgggtcgcg
     1861 aagctgtgac gcagcgcgtg cagcgtcacg cccttgctga tcccggccgc ctcggccgcc
     1921 tcatgaaaca ggcggttgag ctggcgggtg gtcatgggct tgccggccct ggtgccagga
     1981 aatagccaac gttccggcgc aggcgtttgc gcatcctgac gcgatgggcg caccttccac
     2041 cattggcgca agagatcgag cgtctcggat gacagcatga cattgcggtc cttgcggccc
     2101 ttggattgct cgatgcgaat gatcttctgc gcactgtcga tatgtttgac cttcagccgg
     2161 accacctcgc cggcgcgcaa cccacagcca tagccgagac tgagcagaag gcgagccttg
     2221 aggctgcttg cgacggccag caggcgcttc gtctcatccg tactcatcac cagcggaatc
     2281 ttccgaggct cgcggaggtg atagatctcg gccgcaaggt ccaatcgccg cagcgtcacg
     2341 cggaacaaga accgcagccc ggtcatgatg cggttgcggt tgcagatgct cgcgccggtc
     2401 tcggccaagt gcagctggaa gcggcggatg tcctcaagtg tggccgtgtc tggggagcgc
     2461 ttgagaaacg cagcgaaccg tttgcagctg ctgatatggc ccctctgcgt gcccgcgcac
     2521 agcttgcgcg cgttcatgtc ctctatcatg cgctggcgaa gcggactgat agtacccgtg
     2581 gtcatacacg actcctgtct gggatgaggt ggatcacacc tcgatcttca agacagggcg
     2641 ccacgcccgc caccgacgat ctctcctctg gaaacaagct cgcggtcagg ccgcccgcct
     2701 cagcgggcta tcgcgcagcg atttagtgct gtggcccatt gcgaccaagg ccgcctgccg
     2761 cctggatgtc ggctatgagg agtagacagg acttcacctg cgcccggccg aaacgacgcg
     2821 aatgacccaa gaccgacgtg tctgaacatg cagtgcactc ccgactgctt cgcccgacgc
     2881 gccgtggtga tggttctaga acgaggcagc aagttctgac atccaggttg gcatgcggcc
     2941 tacaaggacc gtcgctgcca tcttgtccca gcctgtcagg atgaagacgc ccgttacggt
     3001 aagtgcgcta cccagaatga gcctgccggt gcttccggcc gaaagcatac gagtcctcca
     3061 gcgcataagt gcttcccgcg acgcgaagcc gagcgccagc agcggcaacg cggttccaac
     3121 cgcaaaaaac agcatcgtca aagcaacctg aagaaggttg cggccctgcg cggcgagcac
     3181 ggaagcggcg cccagtgtag gcccggcaca aggcgcccac accgcgccaa gcagcaggcc
     3241 gacgccaaac tgtccggaaa gcccggaccg ggacccgcca ccaaaccggc tttcaaccca
     3301 gtttcccgga ggcgccgt
//
LOCUS       DBNGZE010000209         4431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1696949, whole genome shotgun sequence.
ACCESSION   DBNGZE010000209 DBNGZE010000000
VERSION     DBNGZE010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1696949"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="AC16FV_05460"
     CDS             <1..1896
                     /locus_tag="AC16FV_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HIR2849541.1"
                     /translation="GISSSAPATPVAVVATSDNSVYDAAVTDPTILGLPASATSTDVS
                     TLVNWMRGRDAYDRNANSNITETRSWNFADPLHSRPVAMTYGCVGGGACTSSSTPIIK
                     LFLGANDGTIRIINNNTGAEEWAFIPQELLSEQYALSQDADGDHIYGTDDSPAFLVND
                     LNNDGVIDPAVGDKVYMYIGMRRGGRNIYAFDVTPSAKMTSQSDTVTPKLVWAILGGT
                     GNFTKLGQTWSRPVVARIRAKCTGSVCDDGNPATDDSESRMAMILGGGYDTNEDNGIP
                     AGTDGMGNAIYIVDPLTGERIWWASSDTSASLVLSNMQYAIPSEVTAVDSNNDKSVDR
                     IYVGDMGGQVWRIDLGDQVGAASNDGSSGFVFADVGCTGGTRMSCTATANQDRRKFFY
                     PPDVAQVDDPNFSVNPYYDLVTIASGDREDPLDLLTTNLSSGREAVHNRIYAIRDYNY
                     KTGKPTTFPPAVITETDMFDNTTDAFGDPSAPSYATSVAAIRTSKGWYIDLKEQTSVA
                     LTNGLTTQWVGEKSLAKTLIYAETLYVTTFVPANDSTAANTCQANEGEGRYYALNYLN
                     GSAAYDLDGNAGNDRFAVVGGGIPSEVIIVIRDGGVTGLVGTSGGAAATKVKGDLPRY
                     KTYWYDE"
     gene            1991..2473
                     /locus_tag="AC16FV_05465"
     CDS             1991..2473
                     /locus_tag="AC16FV_05465"
                     /inference="COORDINATES: protein motif:HMM:NF028042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR2849542.1"
                     /translation="MRRTQRGFTLIELMIVVVIIGILAAIAYPSYNRYSTQTRRSDAK
                     IALTRAASLQEKFFSDCNWYAMTPNGARACGTAGNAASILGIPLTSPEGHYTLAFAAG
                     NIAGGCATYSCGFTITATPVAGGRQAGDGTFRIDALGTKQWDRNNNGAYEATENSWGK
                     "
     gene            complement(2558..3487)
                     /gene="ispH"
                     /locus_tag="AC16FV_05470"
                     /gene_synonym="lytB"
     CDS             complement(2558..3487)
                     /gene="ispH"
                     /locus_tag="AC16FV_05470"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR2849543.1"
                     /translation="MQVYLANPRGFCAGVDRAIEIVERALELFGAPIYVRHEVVHNRF
                     VVDDLRAKGAVFVDELDQVPSGATVIFSAHGVSRAVQKEAAQRGLRVFDATCPLVTKV
                     HMEVVRHRNNGTECILIGHAGHPEVEGTMGQADNGMYLVESVEDVARLQVKDEANLSY
                     VTQTTLSMDDTAKIVSALRARFPNIKGPKKDDICYATQNRQDAVKTLAKSVDVMLVVG
                     SRNSSNSNRLREVAEGRGVRAYMIDGPQDIRREWLTGANNVGLTAGASAPERLVQQVI
                     AQLREWGAEVRGEEEAGTESVVFPVPKALRARD"
     gene            complement(3629..3874)
                     /locus_tag="AC16FV_05475"
     CDS             complement(3629..3874)
                     /locus_tag="AC16FV_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849544.1"
                     /translation="MSMYVKFFMSLAAAAMLSASPWASAVDRPNNQKIDPNDPVVKCQ
                     KDCQREKDSERYESCMLKCNELGKHQIPIPAVPNQKK"
     gene            complement(3998..>4431)
                     /gene="lspA"
                     /locus_tag="AC16FV_05480"
     CDS             complement(3998..>4431)
                     /gene="lspA"
                     /locus_tag="AC16FV_05480"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR2849545.1"
                     /translation="QLTKFLAADYLTRHGEIHLTPFLNLVLVHNTGAAFGFLGSAGGW
                     QNIFFIIVAVVASIFILWLTWRLDSKDKLLAVALMLVLGGALGNLTDRLIHGYVIDFV
                     DVYYQNRHWPAFNVADSAITIGAVLLLLDTLNRSPGKKRQD"
BASE COUNT         1006 a         1349 c         1214 g          862 t
ORIGIN      
        1 gggatatcct cgtctgcccc ggctacaccc gtcgcggtgg tcgccacctc cgacaattcg
       61 gtttacgacg ccgcggtaac cgacccgacc atccttggcc tgcctgcctc cgccaccagt
      121 accgacgtca gcacgctggt caactggatg cgtggccggg acgcctacga caggaatgcc
      181 aacagcaaca tcaccgaaac gcggtcatgg aacttcgccg atccgttgca cagccgtccg
      241 gtggccatga cctacggatg tgttggcggt ggtgcgtgca ccagcagttc tacgccgatc
      301 atcaaattgt ttctcggcgc caatgacggc acgatccgca tcatcaacaa caataccggc
      361 gcggaagaat gggcgtttat cccgcaagaa ttgctcagcg agcaatacgc gctctcgcag
      421 gacgccgacg gtgatcacat ctacggcacg gacgactcgc cggctttcct ggtcaatgac
      481 ctcaacaatg acggtgtcat cgatcccgcc gtgggcgaca aggtatatat gtacatcggc
      541 atgcgccggg gtggacgcaa tatatacgcc ttcgacgtga caccatcggc caaaatgact
      601 tcgcagagcg atacagtcac gcccaagctt gtgtgggcaa tcctgggcgg tacgggcaat
      661 ttcacgaagc tgggacaaac ctggtcgcgg ccggtggtgg cgcgcatccg cgccaaatgc
      721 actggcagcg tctgtgacga tggcaacccc gccactgacg acagcgagtc cagaatggcg
      781 atgatcttgg gcggcggtta cgacaccaac gaggacaatg gcatccccgc cggcacggac
      841 ggcatgggca acgccattta catcgttgat ccgctcaccg gggaacgcat ctggtgggcc
      901 agcagcgata cctccgcctc gctggtgctg tccaatatgc agtacgccat tccatctgaa
      961 gtcaccgcgg tggactccaa taacgacaag tcggtggatc ggatttacgt aggggatatg
     1021 ggcgggcagg tctggcgaat tgatctgggc gatcaggtcg gcgccgccag caacgacggc
     1081 tcctcgggat tcgtgttcgc cgatgtcgga tgcacaggcg gcacacggat gtcctgcacg
     1141 gcgaccgcca accaggaccg gcgcaagttc ttctatccgc cggacgtggc gcaggtcgac
     1201 gatccgaact tctcggtgaa cccgtactac gatctggtga cgatcgcttc cggcgaccgc
     1261 gaagaccctc tggatcttct gacaacgaac ctgagttcgg gtcgggaggc ggtgcacaat
     1321 cgcatctacg ccatcaggga ctacaactac aagaccggaa agccgacgac ctttccgccg
     1381 gcggtgatca cggagaccga tatgtttgac aacacgaccg acgcgtttgg ggacccatcg
     1441 gcaccctcat acgcaacatc ggttgcggca atcaggacgt caaagggctg gtacattgat
     1501 ctcaaggaac agaccagcgt tgccctgact aacggtctca cgacgcagtg ggtgggtgaa
     1561 aagagcctgg ccaagacgct catctacgcc gagaccctct atgtcaccac cttcgtgccg
     1621 gccaacgaca gcacggcggc gaacacctgc caagccaatg aaggcgaagg gagatattac
     1681 gctctcaatt atctcaacgg atcggcggcc tacgatctcg acggtaatgc agggaatgac
     1741 aggtttgccg tcgtcggcgg cggtattccg tccgaagtca tcatcgtcat ccgtgacggc
     1801 ggcgtgaccg gtttggtggg caccagcggc ggcgcggcgg cgaccaaggt caaaggcgac
     1861 ctgcctcgct acaagacgta ctggtacgat gagtagtcta aaataacgtt gtactcataa
     1921 tgaccatctc ctccgggcac ggaggagatg gtttttatgg caaccacgaa aacacagaag
     1981 gaatatcgct atgcgacgca cgcaacgggg attcactctc atcgagctga tgatcgtcgt
     2041 ggtcatcatc ggcatactcg cggccatcgc ctatccgagc tacaaccgct actcgacaca
     2101 gacgcggcgc tccgacgcga aaattgccct tacccgggcc gccagtctgc aagaaaagtt
     2161 cttctccgat tgtaactggt acgctatgac acctaatgga gcgcgtgcgt gcggcaccgc
     2221 cggaaacgcc gcctcgattc ttggcatacc cctcacttcg ccggaagggc attacacatt
     2281 ggcctttgcc gccggcaata tcgccggtgg ttgtgcgacc tacagttgcg gtttcaccat
     2341 caccgctacc ccggtggctg gcgggcgcca ggccggcgac gggacattcc gaatcgacgc
     2401 cttggggacc aaacagtggg accgaaacaa taacggcgct tacgaggcaa ccgaaaacag
     2461 ctggggcaaa taaatctcac tcactgaaaa gtgggggact gttacataac cgctattcag
     2521 gaattccccg gagcgtcgca ttaatttgca acaaacatta atcccgggcg cgtagcgcct
     2581 tgggcacggg aaacaccacc gactccgtgc ccgcttcctc ctctcctctg acctcggcac
     2641 cccattcgcg caactgcgcg ataacctgct gcaccagccg ttccggggcc gaagcacccg
     2701 cggtaagccc cacattgttc gcgccggtca gccattcgcg ccggatgtcc tgcggcccgt
     2761 caatcatgta ggcgcgcacg cctcgccctt ccgccacctc acgcaaacga ttggaattcg
     2821 agctgttgcg cgagccgacc accagcatta catcaactga ctttgccaga gtcttcacgg
     2881 catcctggcg gttctgggtg gcataacaga tgtcgtcttt cttcggtcct ttgatgttcg
     2941 ggaatcgcgc ccgcaaagcg gagacgatct tcgccgtatc atccatggac agcgtcgtct
     3001 gggttacgta cgacaggttg gcttcatctt tcacctgaag tctggcgaca tcctcgaccg
     3061 attccaccag atacatcccg ttgtcggcct gtcccatggt gccttccact tcgggatggc
     3121 cggcatggcc gatcaggata cattcagtgc cgttattgcg gtggcgcacg acttccatgt
     3181 gcaccttggt taccagcggg caagtggcat cgaatacccg cagacctcgc tgcgcggcct
     3241 ccttctgtac tgcgcgggaa acgccatggg cgctgaagat cacggtcgcc cccgaaggca
     3301 cctgatcgag ttcatcgaca aaaaccgcgc ccttggcccg gagatcgtcg accacgaaac
     3361 ggttgtgcac cacttcatga cgcacataga taggcgcgcc gaaaagttcc aacgcgcgct
     3421 cgacgatctc gatagcacgg tccacgccag cgcaaaaacc gcggggattg gccagatata
     3481 cctgcatggc gccatgatac cctgttttgg cgccaacttc aggggcttgg caagttcacc
     3541 ggtaaacgcg actcatacac atcttaagat gccgaaaaaa caacgcccgg ttagccgggc
     3601 gttgaggaca acaactattg agatcgcgct actttttctg attcggaaca gcggggatgg
     3661 gaatctgatg cttgcccagc tcgttacact ttagcatgca cgactcgtag cgctcactgt
     3721 ctttctcgcg ttgacaatcc ttctggcact tcaccaccgg atcattggga tcgattttct
     3781 ggttgttggg gcgatcgacc gcgctcgccc atggagatgc cgacaacatc gctgcggcgg
     3841 ccaacgacat aaaaaatttc acatacatgg acatatcaga aactccttat agagaaaacg
     3901 agtatggcgc caacctgcat gagccattgc cctggttaca cgtctaaatt ctagccgcgt
     3961 gccgattatt tgccataaac gcttgtcggc gcgtttttca atcctggcgt ttcttccccg
     4021 gggaccggtt cagggtatcg agcagcaaca gcacggcacc gatggtaatc gcggaatcgg
     4081 ccacgttgaa cgccggccaa tgccggtttt ggtagtagac atcaacgaaa tcaatcacat
     4141 aaccatggat cagacgatca gtgagattgc ccagcgcgcc gcccagcacc agcatgagcg
     4201 caacagcaag cagcttgtct ttgctatcca ggcgccaagt cagccagaga ataaaaatac
     4261 tcgccaccac ggcaacaata atgaaaaaaa tattctgcca cccgcccgca ctgcccagaa
     4321 aaccgaatgc cgcaccggta ttgtggacca atacaagatt caggaaaggc gtaagatgaa
     4381 tctcgccatg ccgggtcaga taatcggcgg caaggaattt cgtcagctga t
//
LOCUS       DBNGZE010000210         4796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1279790, whole genome shotgun sequence.
ACCESSION   DBNGZE010000210 DBNGZE010000000
VERSION     DBNGZE010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1279790"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /gene="guaB"
                     /locus_tag="AC16FV_05485"
     CDS             <1..1205
                     /gene="guaB"
                     /locus_tag="AC16FV_05485"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR2849546.1"
                     /translation="KKFEAGIISNPITVTPDTTVRKVLELTRAQHISGVPVTQGDKLV
                     GIVTSRDLRFETRYDEPVSRIMTPQDKLVTVKEGASRDEILKLLHQHRIEKILVVDEK
                     FGLKGLITVKDIQKSTEYPRSAKDPKGRLLAGAAVGVGAGTEERVEMLVDAEVDVIVV
                     DTAHGHSQGVLDRVRWIKKHHPDMQVLGGNIATAEAAKALRDAGADGVKVGIGPGSIC
                     TTRMVAGVGVPQLTAISNVVEALKGTSIPVIADGGVRYSGDIAKALAAGAHTIMIGSL
                     LAGTDESPGQVEIYQGRSYKSYRGMGSLGAMGEGSSDRYFQEHAKGDKLVPEGVEGRV
                     PYKGPMINIIHQLMGGLRSSMGYTGSATIEAMRTKTQFVRITSAGIRESHVHDVMVTK
                     EAPNYQRE"
     gene            1330..2904
                     /gene="guaA"
                     /locus_tag="AC16FV_05490"
     CDS             1330..2904
                     /gene="guaA"
                     /locus_tag="AC16FV_05490"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR2849547.1"
                     /translation="MTDPHSQRILILDFGSQYTQLIARRVREAGVYCELHSWDSPIEE
                     IRQFAPRGIILSGGPESVTLDQTPRAPKEIFDMKVPILGICYGQQTMAAQLGGAVEAG
                     DKREFGYARVRMHGHSKLFRDLRDEKEESGAEWLHVWMSHGDKVTKLPPGFKVIAETP
                     SAPFAGIADEARGFYGIQFHPEVTHTTQGRSILQRFVHDICGCPSLWTPGNIIESMVQ
                     SVRAQVGKDEVILGLSGGVDSSVVAALLHRAIGKQLTCIFVDNGLLRLNEGDQVMETF
                     ARHMGVKVLRIDAEERFLSALKGVSDPEKKRKIIGGIFIDVFEEEAAKIKNARWLAQG
                     TIYPDVIESAAAKTKKAHVIKSHHNVGGLPEKMNIKLLEPLRELFKDEVRRIGEELGL
                     PHQMIYRHPFPGPGLGVRILGEVKKEYADLLRKADAIYLEELYRHNLYDKISQAFAVF
                     LPVKSVAVLGDARAYDYVVALRAVETVDFMTANWAHIPYEVLGTISNRIINEVRGISR
                     VVYDISGKPPATIEWE"
     gene            2895..3362
                     /gene="tadA"
                     /locus_tag="AC16FV_05495"
     CDS             2895..3362
                     /gene="tadA"
                     /locus_tag="AC16FV_05495"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR2849548.1"
                     /translation="MGVTATDARDEAYMRRALDLARHAEQAGEVPVGALVVLNDEVIG
                     EGWNQPIVSHDPTAHAEIVALRAAAARMKNYRLTDTTLYVTLEPCAMCAGAIVQARVA
                     RVVYGAADPKAGAAGSVFNLLESSSLNHRAQITGGVLAEECGQSLKAFFQARR"
     gene            complement(3365..>4796)
                     /gene="mltF"
                     /locus_tag="AC16FV_05500"
     CDS             complement(3365..>4796)
                     /gene="mltF"
                     /locus_tag="AC16FV_05500"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HIR2849549.1"
                     /translation="QRIGRFHAVSPYLRPVLLLALFIPSLLSCSLFEKNKLQSIIASG
                     ELVVLTRTSPTTYYETPEGPAGFEHDLVKSFAESLGVNPRFIVAEKFSDIMPRFLKGE
                     ADMAAAGITVTESRARLVQFSPPYQEVRQQVIYRLGTNRPPANVQEMIGHQIEVQTGT
                     SYVERLNEFKLEYPELKWTEVDKETEELLQTVWEGLLEFTIADSNIVAVSRQYFPDLQ
                     VAFSLPKPESLAWAFPQGQDQSLHDAAMKFLAKSRASGELAQLIDRYYGPASRSNFIN
                     LSVYKIRLRSVLPRYQLLFEKAGKEYGLDWRLLAAIGYQESFWDPEATSPTGVRGLMM
                     LTEETADQLGIANRLDAAQSIDGGARYFQGLITRMPTGITGPDRIWMALAAYNIGISH
                     LEDARILTQKQGHDPNKWNDVKNYLPWLADETWYTKTKFGYARGMEPVLFVNRVRTYY
                     DVLVKIDGEEKARNKPQALDLKAPAI"
BASE COUNT          932 a         1432 c         1512 g          920 t
ORIGIN      
        1 tgaagaaatt cgaggccggc atcatcagca atccgatcac cgtcacgcct gacaccaccg
       61 tccgcaaggt gctggagctg acgcgggcgc agcacatctc tggcgtgccg gtcacgcagg
      121 gcgacaaact ggtcggcatc gtcaccagcc gcgacctgcg cttcgagacg cgttacgacg
      181 agccggtgtc gcgcatcatg acgccgcagg acaagctggt gacggtgaag gaaggcgctt
      241 cgcgcgacga aatcctgaag ctgctgcatc agcaccgcat tgagaaaatt ctggtggtgg
      301 atgagaagtt cggcctcaag ggcctcatca cagtcaagga tatccagaaa tccaccgaat
      361 acccgcgttc ggccaaggac cccaagggcc gtctgctggc cggcgccgcg gtcggcgtcg
      421 gcgcgggcac cgaggaacgc gtggagatgc tggtggacgc cgaggtggac gtgatcgtgg
      481 tggacaccgc gcacggccat tcgcaaggcg tgctcgaccg ggtgcgctgg atcaagaaac
      541 atcatcccga catgcaggtg ctcggcggca acatcgccac cgccgaggcg gccaaggcgc
      601 tgcgtgacgc cggcgcggac ggagtgaagg tcggcatcgg cccgggttcc atctgcacta
      661 cgcgcatggt ggccggcgtc ggcgtgccgc aactcacggc catatccaat gtggtggaag
      721 ccctgaaagg caccagtatt ccggtgatcg ccgacggcgg cgtccgctat tccggcgaca
      781 tcgccaaggc gctcgccgcc ggcgcgcaca cgatcatgat cggcagcctg ctggccggca
      841 ccgacgaatc gcccggccaa gtcgagatct atcagggccg ctcctacaaa tcctaccgcg
      901 gcatgggttc gctcggcgcc atgggcgaag gttcgagcga ccgttacttc caggaacatg
      961 ccaagggcga caagctggtg ccggaaggcg tcgagggtcg cgtgccctac aaggggccga
     1021 tgattaacat cattcatcag ctcatgggcg gcctgcgttc cagcatgggc tataccggca
     1081 gcgcaaccat tgaagcaatg cgtaccaaga ctcagttcgt gcgcatcacc agtgccggca
     1141 tccgcgagtc gcacgtgcac gacgtgatgg tgaccaagga agcgcctaat tatcagcgtg
     1201 aatgattcac aaccgccagg gcgccagggt cgtcaggaaa agatcaaaca gggttgttcg
     1261 tgcgcagtgt gatttctttg gtttccctgg cgttcctggc gtcttggcgg tgaaaactaa
     1321 tatcaagcca tgaccgaccc acattcccag cgaattctca tcctcgattt cggttcccag
     1381 tacacgcaac tgatcgcgcg ccgcgtgcgc gaggccggtg tttattgcga gctgcattcc
     1441 tgggatagcc caatcgagga aatccgtcag ttcgccccgc gcggcattat tctttccggc
     1501 ggcccggagt cggtgacgct ggaccagacc ccgcgcgccc cgaaggaaat cttcgacatg
     1561 aaggtcccca tcctcggcat ctgctacggc cagcagacca tggcggcgca actgggtggg
     1621 gcggtcgagg ccggtgacaa gcgcgagttc ggctatgcgc gggtgcggat gcacgggcat
     1681 tccaaacttt tccgcgatct gcgcgacgag aaggaggaat ccggcgcgga gtggctgcac
     1741 gtgtggatga gccacggcga caaggtcacg aagctgccgc cgggcttcaa ggtcatcgcc
     1801 gaaaccccgt ccgcgccgtt tgccggcatc gccgacgaag cgcgcgggtt ctacggcatc
     1861 cagttccatc ccgaggtgac gcataccacg cagggacgtt caatactgca acgcttcgtg
     1921 catgacatct gcggctgtcc gtcgctttgg acgccgggca acatcatcga gagcatggtg
     1981 cagtcggtgc gtgcgcaggt cggcaaggac gaggtgatac tcggcctgtc gggcggcgtg
     2041 gattcctccg tggtcgcggc gctgctgcac cgcgccatcg gcaagcagct gacctgcatc
     2101 ttcgtggaca acgggctgct gcgcctgaac gagggcgatc aggtgatgga aaccttcgcg
     2161 cgtcacatgg gcgtgaaggt gcttcggata gacgccgaag agcggtttct gtcggcgctc
     2221 aagggcgtgt ccgatccgga aaagaaacgc aagatcatcg gtggcatctt catcgatgtt
     2281 ttcgaggaag aggcggccaa gatcaagaac gcccgctggc tggcgcaggg gacgatttac
     2341 ccggacgtga tcgaatccgc cgcggccaag accaagaagg cgcacgtgat caagtcgcac
     2401 cacaacgtcg gcggcttgcc cgaaaagatg aacatcaagc tgctcgaacc gttgcgcgaa
     2461 ctgttcaagg atgaggtacg ccgcatcggc gaggaactgg gactgccgca ccagatgatc
     2521 taccgccatc ctttccccgg accgggtttg ggcgtgcgga tcttgggtga ggtaaaaaag
     2581 gaatacgccg atctgctgcg caaggcggac gcgatttacc tcgaagagct gtaccgtcac
     2641 aatctttacg acaagatcag ccaggcgttc gcggtgttcc tgccggtcaa atccgtggcc
     2701 gtgctcggcg acgcgcgcgc ctacgattat gtcgtcgcgt tgcgcgcggt ggagacggtg
     2761 gatttcatga ccgccaactg ggcgcatatt ccttacgaag tcctcgggac gatttccaac
     2821 cgcatcatca acgaagtgcg aggcatctcg cgcgtggtgt acgacatctc cggcaaaccg
     2881 ccggccacca tcgaatggga gtgacggcaa cggacgcgcg tgacgaggcg tacatgcgcc
     2941 gcgcgctcga tctggcccgt catgccgaac aagccggtga agttccggtg ggcgcgctgg
     3001 tggtgctgaa cgatgaagtg atcggcgaag gctggaacca gccgattgtc tcgcacgatc
     3061 ccaccgccca tgccgagatc gtggccttgc gcgccgccgc cgcgcgcatg aaaaattacc
     3121 ggttaacgga taccaccctt tatgtgaccc tcgaaccctg cgccatgtgt gccggagcca
     3181 tcgtgcaggc gcgcgtcgcg cgtgtggtgt acggcgccgc cgatccgaag gctggcgccg
     3241 ccgggagcgt gtttaatttg ctggaatcat cttcgctgaa tcatcgcgcg caaatcacgg
     3301 gtggtgtgtt ggcagaggag tgcggacaga gtctcaaggc attttttcag gcacggcgtt
     3361 agactcagat cgccggcgcc ttgagatcga gcgcctgggg tttgttcctg gccttttctt
     3421 ccccgtcgat ctttaccaga acgtcataat acgtccgcac ccggttgacg aagagcaccg
     3481 gttccatccc gcgggcatag ccgaattttg tcttggtgta ccaagtctcg tcggcgagcc
     3541 acggcagata attcttcacg tcgttccact tgttcgggtc gtgcccctgt ttctgcgtga
     3601 gaatgcgcgc gtcttccaga tggctgatgc cgatgttgta ggccgcgagc gccatccaga
     3661 tccggtccgg cccggtgatg ccggtcggca tgcgcgtgat cagtccctga aaatagcgtg
     3721 cgcctccatc aatgctctgg gccgcatcca gacgattcgc gatacccagt tgatcggcgg
     3781 tttcctcggt cagcatcatc agtccgcgca cgccggtcgg cgaagtagcc tccgggtccc
     3841 agaaggattc ctgatagcca atggcggcga gcaggcgcca gtccagtcca tattccttgc
     3901 cggccttttc gaatagaagc tgatagcgcg gcagaacact gcgcaggcgt atcttgtaga
     3961 cgctcagatt gatgaaattc gaccggctgg ccgggccgta atagcggtcg atcagctgcg
     4021 ccagttctcc cgatgcccgg ctcttcgcca gaaatttcat ggccgcgtca tgcaggcttt
     4081 ggtcctggcc ctgggggaag gcccaggcca gcgattccgg cttcggcaaa ctgaaagcta
     4141 cctgaagatc gggaaaatac tgccggctca ccgccacgat gttcgagtcc gcgatggtga
     4201 attcaagcag tccttcccac acagtttgca gcagctcctc ggtttcctta tccacctcgg
     4261 tccatttcag ttccgggtat tccagtttga attcattcag gcgctcgacg tagctggtgc
     4321 cggtctgcac ttcgatctgg tgcccgatca tctcctggac gttggctggg gggcggttgg
     4381 tccccagccg gtagatcact tgttgccgga cttcctggta gggcgggctg aattgcacca
     4441 gccgggcgcg cgattcggtc acggtgatgc cggcggccgc catgtcggct tcgcccttga
     4501 gaaaacgggg catgatgtcg ctgaattttt cggccacgat gaagcggggg ttcacgccca
     4561 gtgactcggc gaaacttttc accagatcgt gttcgaaccc ggccggccct tccggcgttt
     4621 cataataggt ggtcgggctc gtgcgcgtca gcaccaccag ttcgccgctg gcgatgatgg
     4681 attgcagctt gtttttctcg aacagcgagc aggacaggag agaggggatg aacaatgcca
     4741 gcagcaggac agggcggaga tatggcgaca cggcgtgaaa ccgtccaatc ctttgt
//
LOCUS       DBNGZE010000211         3695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1627906, whole genome shotgun sequence.
ACCESSION   DBNGZE010000211 DBNGZE010000000
VERSION     DBNGZE010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1627906"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="AC16FV_05505"
     CDS             complement(<1..1370)
                     /locus_tag="AC16FV_05505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2849550.1"
                     /translation="MIWQSLRLRLIAGGRVAILLALSLSGAGLVLLFERHVTRTIADD
                     LNVILKQVIAAIDVDAQGQLIVTSPPADPRFAEPLSGLYWQVTDDRGELLRSRSLWDT
                     VLKLARDTPAPGEVHQHATDGPAERRVLVVERMIQLTVAGRRMPVRVAVAGDLGRVAA
                     AATAFSWDLAAALAILGLVLVTATAIQVTLGLRPLASLRQGVADIRAGRIRYLAPAAP
                     VEVQPLVAEVNSLLDEQERVIERSRSRAADLAHGLKTPLAALAGDVRRLRAEGQTSIA
                     SDIESVAEDMSRHVDRELARARVGAVRRNVSVTTELAPLVQSLVGTLVRTPSGARVLF
                     EQRIVDALFLPIDRTDLAEVLGNILENAARHAKSRVRITAQAASMIVIEDDGPGVSAE
                     ARTRVLERGIRLDQRKDGAGLGLAIVQDVLEVYGWRLVLGASHDLGGLQATIAPVSAS
                     ISDQNVS"
     gene            complement(1367..2032)
                     /locus_tag="AC16FV_05510"
     CDS             complement(1367..2032)
                     /locus_tag="AC16FV_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2849551.1"
                     /translation="MRVLLVEDDRRIAADVSQALQASGYVVETVGDGEEAWFRGDTED
                     YGAIILDLGLPKMDGLSVLKRWRANGRHMPVLILTARGSWAERVDGIDAGADDYLPKP
                     FQMEELLSRLRAIVRRASGQASSVVAVGDVTLDERQMKVNVRGLPIALSPLEYRVVAY
                     LVRRRGRIVSPQELDENVYGQGEEHGSNALEVLVGRVRRKLGVGLIETRRGFGYIVPD
                     SPP"
     gene            complement(2032..2331)
                     /locus_tag="AC16FV_05515"
     CDS             complement(2032..2331)
                     /locus_tag="AC16FV_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR2849552.1"
                     /translation="MLLALAALFAATLQLADARDQDALRRDQVRRAVEAGEIRSLADI
                     LKAARDKLPGEVAGVKVEHEEGRWFYEFRVVDGRGRLFEVYIDARSGEIERIKEK"
     gene            2520..2849
                     /locus_tag="AC16FV_05520"
     CDS             2520..2849
                     /locus_tag="AC16FV_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR2849553.1"
                     /translation="MEKISSFKVGAAILGFSALLKISVFAIVIAMLGITSPAQAGSLG
                     RPCTDAPQKQWLSVEALQSKVEALGFKVQMAKVKNCCGELYALNKLGNRVELFVDPSD
                     GHVVGLL"
     gene            2849..3421
                     /locus_tag="AC16FV_05525"
     CDS             2849..3421
                     /locus_tag="AC16FV_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR2849554.1"
                     /translation="MVLLVSLVDNAIDAGGATPPAMVKVWDPFVRVFHWSLAALFVIA
                     YVTGDEVERVHIAAGYAIAGLLALRIVWGFIGPRHARFADFLRSPREVLAYLRDALLF
                     RTLRYVGHNPAGGLMVITLIVMLAGTCLTGYLMTTDAYWGSEVLDEVHGAMANATLAL
                     VMLHVLGVLVASFEHRENLVKAMVTGRKRQ"
BASE COUNT          700 a         1203 c         1123 g          669 t
ORIGIN      
        1 gaaacgttct gatcagagat cgaggccgaa accggtgcaa tcgtggcctg caagccccct
       61 aggtcgtgcg aggcgccaag gacgaggcgc cagccgtata cctcaagaac gtcctgcacg
      121 attgcgagcc cgaggccggc gccatccttg cgctggtcca gccgtatccc gcgttccaac
      181 actctcgtcc gggcctccgc gctgacaccc gggccatcgt cctcaatgac gatcatcgaa
      241 gccgcctgcg cggtgatacg tacccgcgat ttggcgtggc gggcggcatt ctccagaatg
      301 ttgccgagca cctcggcgag atcagtacgg tcgatcggca aaaaaagtgc atccactatg
      361 cgctgctcga aaaggacgcg agcgccagag ggtgtgcgca cgagcgtgcc gacgagcgat
      421 tgcaccagtg gcgccaattc ggtggtcaca ctgacattgc ggcgcacggc cccgacgcgc
      481 gcgcgcgcca gttccctgtc cacatgccgg ctcatgtctt cggccacgct ttcgatgtcg
      541 cttgcgatgc tggtttgccc ttctgctcgc aaacgccgaa catcgccagc gagcgcagct
      601 agcggtgttt tcaacccatg tgccagatcc gccgcacggc tgcgcgaccg ctcgatcacg
      661 cgctcttgct cgtcgagcag cgaattcacc tcggccacca gcggctgaac ttcgaccggt
      721 gcggccggcg ccaaataccg gatacggccc gcgcggatgt cggccacgcc ctgccgaagc
      781 gaagccagcg ggcgcaggcc cagggttacc tggattgcgg tcgcggtcac gagcaccaga
      841 cccaggatcg ccaaggctgc ggcaaggtcc caggaaaagg ctgtggccgc cgcggcgact
      901 ctccccagat cgccggccac cgcgacgcgt accggcatcc gccggccggc gacggtcagt
      961 tggatcatgc gctccacgac cagcacacgg cgttctgccg gaccgtccgt cgcatgctga
     1021 tgcacttcac ccggagcggg agtatcgcgc gcgagcttga gcaccgtgtc ccacagcgag
     1081 cgcgagcgca gcagttctcc tcggtcgtcg gtgacctgcc aatagagtcc cgaaagcggt
     1141 tcggcaaaac gaggatcggc aggcgggctg gtgacgatca gctgcccttg ggcatcgacg
     1201 tcgatggcgg cgatgacctg cttgagaatg acgttgagat cgtctgcgat cgttcgcgtc
     1261 acgtgccgtt cgaaaagcag cactagcccg gcgcccgaca ggctgagcgc caggagaatg
     1321 gcgacccggc cgccggcaat cagccgtaag cgcagcgact gccaaatcat gggggggaat
     1381 ccggcacgat gtagccgaat ccgcgccggg tttcgatcaa cccaacgccg agcttgcggc
     1441 ggacgcgacc aaccaggacc tccaacgcgt tcgacccatg ctcttcacct tgcccgtaga
     1501 cattctcatc caattcctgc ggcgagacga tgcggccacg gcgcctgaca aggtaggcga
     1561 cgacacgata ttctagcggc gacagcgcga tcggcaggcc gcgcacgttc accttcatct
     1621 gccgctcgtc cagcgtcacg tcaccgacgg cgacgaccga cgacgcttgt cctgacgcgc
     1681 ggcgcacgat ggcgcgaagg cgcgagagca attcctccat ctgaaacggc ttgggtagat
     1741 agtcgtcggc gccagcgtcg atcccgtcga cgcgttccgc ccagcttccg cgcgcggtca
     1801 ggatcagcac gggcatatga cgtccattgg cgcgccagcg cttgagtacc gacagcccat
     1861 ccatcttcgg caggccgaga tcaagaatga tggcaccgta atcctcggta tcgcctcgga
     1921 accaggcctc ttcgccatcg ccaaccgttt cgacgacata gccgctggcc tgcagggcct
     1981 gcgatacatc cgctgcgatc cgccgatcgt cctcgacaag gagcacgcgc atcatttctc
     2041 cttgatacgc tcgatctcac cgctgcgggc gtcgatatag acttcaaaca gccggccccg
     2101 gccgtcgacg acacggaatt cgtagaacca gcggccctct tcgtgttcga ctttgacgcc
     2161 agctacctcg cccggcagtt tatcgcgggc ggctttgaga atatccgcca aggagcgaat
     2221 ctcgcccgcc tcgaccgcgc ggcggacctg gtcgcggcgc agtgcatcct gatcgcgggc
     2281 gtccgccaat tgcaacgtgg ccgcaaagag cgcagccagc gcgagcagca ttgccaatac
     2341 cgttaaatcc cgacgtaagc gcatgacgta tcacaccaaa ttggctgtgg ccgcataccg
     2401 caaacgacct gacatgtcgc tgacatggat caacaggctg ccgtcagcac cactcaggca
     2461 aggtcccgtc aacgtccacc gcatggtgcg gcgggaccgc caactcggag accaaaaaaa
     2521 tggaaaaaat ctcatccttt aaggtaggtg ctgcgattct gggattttcg gcgttgctga
     2581 agatttcggt cttcgcaatc gtcatcgcga tgctcggcat cacgagtccc gcccaagcgg
     2641 gcagcctagg caggccatgt accgacgccc cgcaaaaaca gtggctctcc gttgaggcct
     2701 tgcagagcaa agtcgaggca ctcggcttca aagttcagat ggctaaagtc aagaattgct
     2761 gcggcgaact gtatgctctc aacaagcttg gcaaccgggt cgagttgttt gtcgatccca
     2821 gcgacgggca cgtcgtcggc ctgctctgat ggtgttgctc gtgagcctcg ttgacaacgc
     2881 gatcgacgct ggcggcgcga cgccgccggc aatggtcaag gtctgggatc cgttcgtccg
     2941 cgtgttccac tggtcgctgg ccgcactgtt cgtgatcgcc tatgtaacgg gcgacgaggt
     3001 cgagcgggtg cacatcgccg ccggttacgc tattgccggc ctgctggccc tgcggatcgt
     3061 gtggggtttt atcggtcccc ggcacgcccg ctttgcggat ttcttgcgct cgcctcgcga
     3121 agtgctcgcc tacttgcgcg acgcgttgct gttccggacg ctccgctacg tcggccacaa
     3181 tccggctggc gggcttatgg tgatcacgct cattgtcatg ttggccggca cctgcctgac
     3241 cggctacctg atgacaaccg acgcctattg gggatcagaa gtattagacg aagtgcacgg
     3301 tgccatggcg aatgccacgc tcgcgctagt aatgcttcac gtgctcggtg tgcttgtcgc
     3361 tagctttgag catcgggaaa acctcgtgaa ggcgatggtc actggccgca agcgccaata
     3421 gcagctcaac gaatgtccgc tttgggtcat tagcggacat tgcgacatgt ccacgccata
     3481 tccgctttac ccccgaaagc ggacaatgat tgatggggtc cgcgccgcgc gaggtgcttg
     3541 accgctgtct ggcgaacgcg acaatgcacc cctggggcgg gcgcggtgac cccgcatgtg
     3601 gagccatgac ggacccgggg caaggatcga gtcgttctct gcttaagcgc accgagcacg
     3661 gactcgaagg cgtgatcttc accagtcgct ggttg
//
LOCUS       DBNGZE010000212         2048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1763109, whole genome shotgun sequence.
ACCESSION   DBNGZE010000212 DBNGZE010000000
VERSION     DBNGZE010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1763109"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="AC16FV_05530"
     CDS             complement(<1..728)
                     /locus_tag="AC16FV_05530"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR2849555.1"
                     /translation="MARDQIDMTPIETRDELVAWLEAGCKPKAQFRVGTEHEKFAFTI
                     ADHRPVPYSGKRSIRALLEGMHDLLGWEPIMERENIIGMFDVTGGGAISLEPGGQFEL
                     SGAPYDNVHQTAAELMAHLAQLREVAKPLGIGFLGLGMTPNWTRADMPKMPKGRYQIM
                     TAYMPKVGSLGLDMMYRTCTVQANLDFASEADMVKKLRVSLALQPVATALFANSPFTE
                     GKPNGFLSFRSEIWRDTDNQRAGTL"
     gene            1121..>2048
                     /locus_tag="AC16FV_05535"
     CDS             1121..>2048
                     /locus_tag="AC16FV_05535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR2849556.1"
                     /translation="MPQFTPHQNQALNVVAAWLKSKPGTNGTPQVFRLFGYAGTGKTT
                     LATHIADEADGEVKFAAFTGKAASVMRGKGCRGASTIHSLIYRARESGEEIPSFDLWD
                     EAPASNAELIIIDECSMVDAELGRDLLSFGVPVLVLGDPAQLPPIQGGGFFTEAEPDA
                     MLTEVHRQAQDDPIVRLSMDIRAGEYLEPGRYGETEVIRKADLDPQRVLDADQVLVGR
                     NATRRSYNKRMRERRGFEDDMPSAGDKLVCLRNNRKKGLFNGGLWMVKERGGRKGGTK
                     TGIMTMRLLPDDETATRGVKVSVRPECFTGGIE"
BASE COUNT          396 a          623 c          646 g          383 t
ORIGIN      
        1 agcgtgcccg cacgctggtt gtcggtatcg cgccagattt ccgaacggaa cgagagaaag
       61 ccgtttggct tgccttcggt gaacggagaa tttgcgaaca acgcagtagc caccggttgt
      121 aacgcgagcg atacgcgcag cttcttgacc atgtcggctt cgctggcgaa gtcgaggttc
      181 gcctgtacgg tgcatgtgcg atacatcatg tcgaggccga gcgagccgac cttcggcata
      241 taggcggtca tgatctggta gcggcccttc ggcattttcg gcatgtcggc ccgagtccag
      301 ttcggcgtca tgccgaggcc cagaaaaccg atcccgagag gttttgccac ttcgcgcagt
      361 tgcgcgagat gcgccatcag ttcggccgcc gtttgatgca cattgtcgta cggcgcaccg
      421 gagagttcga actgcccgcc cggctccagg gaaatcgcgc caccgccggt gacgtcgaac
      481 atgccgatga tgttctcgcg ctccatgatc ggttcccaac cgagcagatc atgcatgcct
      541 tcaagcagcg cgcggatact gcgtttgccg gaataaggaa cgggacggtg atcggcgatg
      601 gtaaaggcga acttctcgtg ctcggtgccg acccggaatt gcgcctttgg cttgcagccc
      661 gcttcgagcc aggcgacgag ttcgtcgcgc gtttcgatcg gggtcatgtc aatctggtca
      721 cgagccatac agacgtccgg gcaaaaaggt cgcgacctta aacatgggag ccgggaaagg
      781 gcaacgtgac ggctacgcga atttgcgggc cttgcaggcc cgtatcaccg attcactttt
      841 caaaaagctg acgctgcacg cgccgggcgt gccggctccc ccgccatcag accgggcgcg
      901 agtggggtgc ggctgatgca cccaaggacg atgcggctgg accgccaggg ggagcgcgcc
      961 gcatcttcga cgctgccggc cacccccgcg tctggagacg ctgaccagac acccctcggt
     1021 aacggggcgg gatacgcata ggaaaacata ccttagtcaa gagaattgtt catattttgt
     1081 tctttcgctt gcagcatgct tgttctattg atcgtgcccc atgccgcagt tcaccccaca
     1141 ccagaatcag gccctcaacg tcgtcgccgc ctggctcaaa tccaagcccg gcaccaatgg
     1201 taccccgcag gtgttccgcc tcttcggcta tgccggtact ggcaagacca cactcgccac
     1261 gcacattgcc gatgaggccg atggtgaggt gaagttcgcc gccttcacgg gtaaggcggc
     1321 gtctgtcatg cgcggcaagg gctgtcgcgg cgcctccacc atccacagcc tgatctaccg
     1381 tgcgcgcgag agcggcgagg aaatcccgag ctttgacctg tgggacgagg cgcccgcctc
     1441 gaacgctgaa ctgatcatca ttgacgagtg ctcgatggtt gatgccgagc tcggccgtga
     1501 tctcttgtcg ttcggcgtgc cggtattggt actcggcgat cccgcacagc tgccgccgat
     1561 ccagggcggc ggatttttca cagaggccga gcccgatgcg atgctcacgg aggtgcaccg
     1621 ccaggcacag gacgacccga tcgttcggct gtcgatggac atccgcgccg gcgagtacct
     1681 cgagcctggt cgctatggcg agaccgaagt gatccgcaaa gccgacctcg acccgcagcg
     1741 tgtgctcgat gccgatcagg tactggtcgg acgcaacgcc acgcgtcgtt cctacaacaa
     1801 gcgcatgcgt gagcggcgtg gcttcgaaga cgatatgccc tcggccggcg acaagctggt
     1861 gtgcctgcgc aacaaccgca agaagggtct gttcaatggc ggactatgga tggtgaagga
     1921 acgcggcggg cgcaaaggcg gtaccaagac cggtatcatg acgatgcggc tcttgccgga
     1981 cgacgaaaca gcgacacgcg gcgtgaaggt gtcggtgcgt ccggaatgct tcaccggtgg
     2041 catcgagc
//
LOCUS       DBNGZE010000213         4230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_168861, whole genome shotgun sequence.
ACCESSION   DBNGZE010000213 DBNGZE010000000
VERSION     DBNGZE010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_168861"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="AC16FV_05540"
     CDS             <1..520
                     /locus_tag="AC16FV_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849557.1"
                     /translation="GGRRRTTPSLTSRTESGLGDIALAGSPVLAQDAGWRLGLGGLIK
                     FGTADEQDNLGTGEDDVAVQLEAEKTYGNNTLFGTAGYRILGDPPGIDYDNVFYGSVG
                     VSHQLDANRTVGAELYAQEAPLPGVDGKSELTLFLSGKSGPKTRLTGYLIAGFADGSP
                     DWGMGVALKMSQ"
     gene            complement(530..979)
                     /locus_tag="AC16FV_05545"
     CDS             complement(530..979)
                     /locus_tag="AC16FV_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR2849558.1"
                     /translation="MLVGKICNREVVFVEPDTSIAEAARLMREHHVGGLVVIKEKSGK
                     RVPVGIITDRDLVIEVIAEGVEMGDISVGDIMSDQLVTARESDDLLETIKMMRAKGIR
                     RLPVIDDDGALAGILTVDDLIDLFSEQIADLARLIAFEQKREQEKRK"
     gene            1283..1942
                     /locus_tag="AC16FV_05550"
     CDS             1283..1942
                     /locus_tag="AC16FV_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR2849559.1"
                     /translation="MFDLFVDSLIILLVVVDPVAVAPLFVALTHGESAASKRRIALRG
                     TFIAGGILVVFALAGGTLLDALGIGMPAFQIAGGALLFLLAVDMVFARHSGLRSTTER
                     EQREAETKKDISVFPLAIPLIAGPGALTSVLLMVGEQGDDPAVIGTVLMVILVVLLVT
                     LVSLMASARIMAFMGETGANVVSRVLGVVLAALAVQFVLNGWQEGFMPIEENVSQMLT
                     V"
     gene            complement(1955..3226)
                     /locus_tag="AC16FV_05555"
     CDS             complement(1955..3226)
                     /locus_tag="AC16FV_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR2849560.1"
                     /translation="MSKTKLDVQQLKIGMYVRELDRPWRDTPFLFQGFEIRSEDDIRE
                     LRRHCQHVYIDIPETYQKPAPRTPAERAVAEEMLQHKHVERDLLIKVASHPLLKPVYH
                     DQTSLEEEIEVVRELHRETRELVYTLLEDVRLGKNINSLAAKKLVGELVESVIRNPDA
                     LTSFAQLKKKDEYTAQHSMRVCILALSFGRHLGLERHELNLLGIGALLHDIGKMKVPN
                     EILNKPGALTEYEYALMKSHVPHGVEILEKSSGIPRPAIEVARCHHERYGGAGYSGGL
                     KGDEIGLFGMIGGIVDCYDAISSDRAYHAGMSAHAALKKMYEWRHRDFHPGLVEQFIQ
                     CMGIYPIGSVVELNTGEVGVVVTMNRTRRLKPRVALVLQPDYYPVPGARTVDLTDYKT
                     RDGRPCEIDRVLDSGAYGIDPVQYLPVNTAA"
     gene            3430..3639
                     /locus_tag="AC16FV_05560"
     CDS             3430..3639
                     /locus_tag="AC16FV_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HIR2849561.1"
                     /translation="MSEHTYKVVELVGSSTQGTDKAIQNAIARAAKTLRNLDWFEVVE
                     TRGHLANGKIAHWQVKLKVGFRMED"
     gene            3649..3963
                     /locus_tag="AC16FV_05565"
     CDS             3649..3963
                     /locus_tag="AC16FV_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR2849562.1"
                     /translation="MLRLCTAANLPEAHLLLHRLAHAGIEARVFNEHAQGGLGEIPFT
                     HAYPEIWIMEPAQEARAREIVAEFERPSVALPPARCSGCGEMNPAGFEICWQCGRPLP
                     AS"
BASE COUNT          768 a         1197 c         1329 g          936 t
ORIGIN      
        1 gggtgggagg cgtcgcacga cgccgagcct gacgagccgg actgaatccg gattgggcga
       61 cattgcgctg gcaggctcgc ccgtgcttgc gcaggacgcc ggctggcgcc tgggcctggg
      121 tggactcatc aagtttggca ccgccgatga acaggacaat ctgggcacgg gagaagacga
      181 cgttgccgtt cagcttgaag cggaaaagac ctacggcaat aacacgctct tcggcaccgc
      241 tggatacagg atcctgggtg atccgccggg aatcgattac gacaatgtct tttacggatc
      301 ggtgggagta tctcaccagc tcgatgccaa tcgtaccgtt ggcgcggaac tgtatgcgca
      361 ggaagcgccg ctccccggcg tggacggaaa gtcggaattg acgttgtttc tgagcggcaa
      421 atcgggaccg aaaacccggt tgacggggta cctgatcgcg ggctttgccg atggcagccc
      481 cgactggggc atgggcgtcg ccctcaagat gagtcagtag cgccgcggat tatttccttt
      541 tttcctgttc gcgcttctgc tcgaacgcga tcaggcgcgc gagatcggcg atctgttccg
      601 agaacaggtc tatcaggtca tccacggtga ggatgccggc cagcgcgccg tcatcgtcga
      661 tgacgggcag gcgccggatg cctttggcgc gcatcatctt gatcgtctcc agcagatcgt
      721 cgctttcgcg cgccgtcacg agctggtcgc tcatgatgtc accgacgctg atgtcgccca
      781 tctccacgcc ctcggcgatg acttcgatca cgaggtcacg atccgtgatg atgccgacgg
      841 gaacgcgttt accggatttt tcctttatta ccaccagacc gccgacgtga tgctcgcgca
      901 tcagccgcgc cgcttccgca attgaggtgt ccggctcgac aaagacaacc tcgcggttac
      961 agatttttcc gacgagcatg gttgcctcct tggtttaaaa ttattctaga acggcgacgc
     1021 gatgtcttct tgatctgtgt caatgtaccg aggcggtgtt tctgccagag tgcgcgcgtt
     1081 attgcctcag cagggcaatc cgccgctcgg tgtctccgca cggccatcac ttctccctgg
     1141 tggcctgatt ttgagcggcg gaattttttt gcccgcgtgt tttcgcgcaa tatcccccga
     1201 ttttccgctt ctgccaactg ttgtttcagg atttccccgc ccgaggtaaa gttagactca
     1261 ttcttgagcg aagggaggcg ccgtgttcga tctgttcgtg gattcgttga ttattctgct
     1321 ggtggttgtc gatccggtag ccgtcgcgcc gcttttcgtg gcgctgacgc acggtgaatc
     1381 ggcggccagc aaacgccgca tcgccctgcg cggcacgttt atcgcggggg ggatcctcgt
     1441 ggtattcgct ctggccgggg gcacgttgct ggatgcgctg ggcatcggca tgccggcgtt
     1501 tcagatcgcg ggcggggcgc tgctcttttt gctcgcggtt gacatggtgt tcgcgcgcca
     1561 ttcggggctg cgctccacca ccgaacgcga acaacgcgag gcggaaacga aaaaggacat
     1621 ttcggttttc ccgctggcga ttccgcttat tgccggaccg ggagcattga cctcggtgct
     1681 gctcatggta ggcgaacagg gcgatgatcc ggccgtgatc ggcacggtct tgatggtgat
     1741 tctcgtggtg ctgctggtca cgctggtctc gctgatggcc tcggcgcgca tcatggcctt
     1801 catgggcgag accggcgcca acgtcgtcag ccgcgtgctc ggcgtggtgt tggcggcatt
     1861 ggccgttcag ttcgtgctga acggctggca ggaaggattc atgccgatcg aggaaaacgt
     1921 gtcgcagatg ctgacggtct gagcatggag aaaatcaggc ggcggtgttg acggggagat
     1981 actgtaccgg gtcgatgccg taggcgcccg aatcgagcac gcggtcgatc tcgcacggcc
     2041 gcccgtcgcg cgttttgtag tccgtgagat ccacggtcct ggcgcccggg accgggtagt
     2101 agtcgggttg cagcaccagc gccacccgtg gtttcagacg gcgtgtgcgg ttcatggtca
     2161 cgaccacgcc gacctcgccg gtgttgagtt ccaccacgct gccgatcgga taaattccca
     2221 tgcactggat gaattgctcc accaggccgg ggtggaaatc gcggtgacgc cactcgtaca
     2281 tttttttcag cgcggcatgg gccgacatgc cggcgtgata ggcgcggtcg ctggagatcg
     2341 cgtcgtagca gtccacgatg ccgccgatca tgccgaacag gccgatctcg tcgcccttca
     2401 ggccgccgct gtagccggcg ccaccgtaac gctcgtgatg acagcgcgcc acctcgatgg
     2461 ccggacgtgg aataccggag gatttttcca ggatttccac gccgtgcggc acgtgacttt
     2521 tcatcagcgc gtattcgtat tcggtgagcg cgccgggttt gttcaggatt tcgttcggca
     2581 ccttcatctt cccgatgtcg tgcaatagag cgccaatgcc gagcaggttg agctcgtgtc
     2641 gttccaatcc gaggtggcgg ccgaatgaca gcgccaggat gcagacacgc atgctgtgct
     2701 gcgcggtgta ttcgtccttc tttttcagtt gcgcgaagct ggtgagcgcg tccgggttgc
     2761 ggatgacgct ttccaccagt tcgccaacca gctttttggc ggccaggctg ttgatgttct
     2821 tgccgaggcg cacgtcctcc agcagggtgt agaccagttc ccgggtttcg cgatgaagct
     2881 cgcggacaac ctcgatttct tcttccagac tggtttgatc gtggtagacc ggcttgagca
     2941 gcgggtggct ggcaaccttg atcagcagat cgcgctcgac atgcttgtgc tgcaacatct
     3001 cctcggcgac ggcccgctcc gccggggtgc gcggcgccgg tttctgatag gtttcgggaa
     3061 tgtcgatgta gacgtgctgg cagtggcgcc gcagctcgcg gatatcgtcc tcgctgcgga
     3121 tttcgaagcc ctgaaagaga aaaggggtgt cgcgccaggg acggtccagt tcgcggacgt
     3181 acatccctat cttgagctgc tgaacgtcga gttttgtttt gctcattgtt ggtaacccgc
     3241 cagttccccg cttgtccata attcggtgcg aaactgctgt cacagctgta tagacattaa
     3301 ttgtaggaca gaatattccg cccccccaag aaagacaccc gtctattccc gatctgtgtc
     3361 ggcagcggac aggaacggga gaagctgcca caatggacac gtccctacac tccatggagg
     3421 aatccgaaca tgagcgaaca tacctataaa gtggtggaat tggtgggttc atccacccag
     3481 ggcacggaca aggccatcca gaatgccatt gcccgcgccg ccaagaccct gcggaatctg
     3541 gactggttcg aggtggtcga gacccgcggc catctcgcga acggcaagat cgcgcactgg
     3601 caggtcaagc tcaaggtcgg cttccgtatg gaggactagg gaggcgggat gctgcgtctc
     3661 tgcaccgcgg ccaatctgcc ggaggcccat ctgctgttgc accgcctggc gcacgccggg
     3721 atcgaggcgc gcgtgttcaa tgaacacgcg caaggcggtc tcggagagat tcccttcacc
     3781 cacgcttatc ccgaaatctg gatcatggaa ccggcgcagg aagcgcgggc gcgcgagatt
     3841 gtggcggagt tcgagcgtcc ttccgtggcg ttgcctccgg cgcggtgctc aggttgcggc
     3901 gagatgaatc cggcggggtt tgaaatctgc tggcagtgcg gcaggccttt accggcgtca
     3961 tgacacgaga cgaagagtgg atgaaaaaat tattttttta agattgtgaa accgcagatg
     4021 aacgcggata aacgcagata gaaaataaat aaacagaatt ccgcaatatt atgttgttat
     4081 ccgcgttaat ctgcgtttat ctgcggttcc aaaaataatt ataatcgtgt gctgaggtcg
     4141 gcctcggcct gcaccttgga gcgcagcagg tgctcggcga tatgcgccca gggcgcacgc
     4201 ttctccgaaa tgatgtcgga aatcttgcgc
//
LOCUS       DBNGZE010000214         1923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1280143, whole genome shotgun sequence.
ACCESSION   DBNGZE010000214 DBNGZE010000000
VERSION     DBNGZE010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1280143"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..960
                     /locus_tag="AC16FV_05570"
     CDS             103..960
                     /locus_tag="AC16FV_05570"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR2849563.1"
                     /translation="MLTVMLTTLVDTETLSRHLDDPRWIVVDCRFVLTDPEAGRRAYA
                     AGHIPGARYAHLNEDLSSPIIPGSGRHPLPFPNALAEKLGRWGIDKTSQVVAYDDLFG
                     AMAARLWWLLRWLGHEAVAVLDAGYPKWAREKRPVTTDPPKIQPTQFHLTINHSMWVD
                     ADRVLEMARAKDGLVLDARAEERFRGEIEPLDKVAGHVPGANNMPYEDNLDFSGEFMS
                     DEALREHYQSVLGKVPPEKVVHMCGSGVTACHSILAMEHAGLSGAKLYAGSWSEWITD
                     PSRPVARGE"
     gene            1079..>1923
                     /locus_tag="AC16FV_05575"
     CDS             1079..>1923
                     /locus_tag="AC16FV_05575"
                     /EC_number="1.13.12.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR2849564.1"
                     /translation="MKRVDDFRLRLGKKELVPIMTGGMGVDISTAELSLVAARLGGIG
                     HISDAMVPTVSDRRFKTGFVKEKQKKYKYNVANRDKSEIHFDLERLAEATRLHVGRTM
                     EAKQGDGLIFINTMEKLTMNSPRETLRVRLSAAMDGGIDGITLSAGLHLGSLALVQDH
                     PRFRDVKFGIIVSSLRALLLFLRKNARLDRLPDYIIVEGPLAGGHLGFGMDWAQYDLK
                     TIVDEIIQYLKQEKLNIPVIPAGGIFTGSDAVEFLEAGAAAVQVATRFTVTKECGLPD
                     KVKQE"
BASE COUNT          401 a          554 c          604 g          364 t
ORIGIN      
        1 ctgccagagt cacgccgcaa gggcggctat taagaatacg tacactcggg ctgtcagcct
       61 ccagcatgca acccgcctcc gggcaggccg gctgatggct gaatgctgac agtcatgctc
      121 accaccctcg tcgatacgga aacgctgtcg cggcatttgg acgacccgcg ctggatcgtc
      181 gtggattgcc gtttcgtcct gaccgatccg gaagcaggcc gacgcgccta cgccgccggg
      241 cacattcccg gcgcgcgcta cgcgcatctc aacgaagacc tgtcatctcc catcataccc
      301 ggcagcggcc ggcatccgtt gccgttcccc aatgcgctgg cggaaaagct cggacgctgg
      361 gggatagata aaaccagtca ggtcgtcgct tacgacgatt tattcggcgc catggcggcg
      421 cgtctgtggt ggttgctgcg ctggctcgga catgaagcgg tggcggtgct cgatgccggt
      481 tatccgaaat gggcgcgtga gaagcggccg gtcacgaccg acccgccgaa aatccagccc
      541 acgcaatttc atctgaccat caaccactcc atgtgggtgg acgctgatcg cgtgctggaa
      601 atggcgcgcg ccaaggatgg gcttgtgctc gatgcgcgcg cggaggaacg cttccgcggc
      661 gagatcgagc cgctggataa agtagcgggc catgtccccg gcgcgaacaa catgccgtat
      721 gaagacaatc tcgacttcag cggcgaattc atgtctgatg aagccttgcg cgagcactat
      781 cagtcggtgc tgggcaaggt cccgccggaa aaggtcgtac acatgtgtgg atcgggcgtg
      841 accgcctgtc acagtatcct cgcgatggaa catgccgggc tttcgggtgc aaaattgtat
      901 gctggcagct ggagcgagtg gatcaccgat cctagccgtc cggtggccag gggcgaatga
      961 aagaaagaat ttatatgatt atttgccgca gagacgcaga gtgcacagag aaaacagttg
     1021 tgaacattga tcggaaaaat ctctgcgatc tctgcgtctc cgcggcgaga actcatacat
     1081 gaagcgcgtt gatgattttc gtctgagact cggcaagaag gagctggtgc cgatcatgac
     1141 cggcggcatg ggcgtcgata tctcgacggc ggagctttcg ctggtcgcgg cgcgcctggg
     1201 tggcatcggg cacatctcgg acgcgatggt gccgaccgtt tccgaccggc gtttcaagac
     1261 cggcttcgtg aaggaaaagc agaagaaata caaatacaac gtcgctaacc gcgacaagtc
     1321 tgaaattcac ttcgatctcg aacgcctggc cgaagccacg cgactccacg tcggccgcac
     1381 catggaagcg aagcagggtg acggcctgat cttcatcaac accatggaaa agctcaccat
     1441 gaacagcccg cgcgaaacgc tgcgcgtgcg tctgtcggcc gcgatggacg gtggcatcga
     1501 cggcatcacg ctgagcgccg gtctgcatct cggttcgctg gcgctggtgc aggaccatcc
     1561 gcgcttccgc gacgtgaagt tcggtatcat cgtttcctcg ctgcgcgcgc tgctgctgtt
     1621 cctgcgcaag aacgcgcgtc tcgaccgcct gccggattac atcatcgtgg aagggccgct
     1681 ggccggcggg catctcggtt tcggcatgga ctgggcgcaa tatgacctca agaccatcgt
     1741 tgacgaaatc atccagtact tgaagcagga aaaattgaat atcccggtca ttccggccgg
     1801 cggtattttc accggcagcg acgcggtgga atttctggaa gcgggcgcgg cggcggtgca
     1861 ggtggcgacg cgtttcacgg tgacgaagga atgtggcctg ccggacaagg tcaagcagga
     1921 ata
//
LOCUS       DBNGZE010000215         3375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_724823, whole genome shotgun sequence.
ACCESSION   DBNGZE010000215 DBNGZE010000000
VERSION     DBNGZE010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_724823"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2584
                     /gene="lon"
                     /locus_tag="AC16FV_05580"
     CDS             164..2584
                     /gene="lon"
                     /locus_tag="AC16FV_05580"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR2849565.1"
                     /translation="MANREELLKTEAEDAAATPAVVESEGGKLARPGDVLPGLLHILP
                     LANRPFFPAQLVPLVVERDPWQRTLEAAVEAGQYVGLVLIRGDDPQTAGPDDFYTMGT
                     ASRIHKVVQLDDKLQLFVEGLQRFRIESWVTRERPFAVRAEYFPETSYKDMSELKAYT
                     VAVINTIKELLPLNPLYGEELKYFLNRFGPDDPARLADFAASLTTSDKQELQEVLEKT
                     HIRERMEKVLVLLKKEVEVAKAQMQIHKNVEESMEKRQREFLLREQMKAIQKELGIAK
                     DDRTAELDKFRARVEDLTLPEEARKRIDEEIEKMSVLEVGSPEYGVTRNYLDWLTQLP
                     WGKYSKDNLDLKNARKILDRDHDGLVDVKDRIIEFLAVGAMKGEIAGSILLLVGPPGV
                     GKTSIGRSIAAALGRKFYRFSLGGMRDEAEIKGHRRTYIGAMPGKFIQAIKDVGVANP
                     VIMLDEIDKIGASYQGDPASALLEVLDPEQNRDFLDHYLDVRFNLSKVLFVSTANQLD
                     TIPAPLLDRTEIIRLSGYITSEKMAIAQHHLIPRQLERAGLKMGQLRIESDAVREIVE
                     RYAREAGVRNLEKRLGQIARKSVVKILNGAKPPIRVGVKNIEEYLGKPLFRPEKPQYG
                     VGVITGLAWTPLGGATLDVEATRVHQATRGFKLTGQLGDVMKESAEIAYSYISSHCKD
                     YGAKANYFEKAFVHLHVPAGATPKDGPSAGVTMATALLSLARGNRIARPVAMTGELTL
                     TGQVLPVGGIREKVIAARRAKISELILPEANRGDFDELPAHIRKGLKTHFASHYRDVA
                     KLVFPRKK"
     gene            2602..2880
                     /locus_tag="AC16FV_05585"
     CDS             2602..2880
                     /locus_tag="AC16FV_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849566.1"
                     /translation="MGRFVSFKCRFCQYEEPSLGVGRGKAAFPFLALYRCNHCHTIGS
                     TWIYENKIPRCSQCYDDALTLLPDDTRRVNCPKCGEPAVIQPKEGCWE"
     gene            complement(2884..3198)
                     /gene="cyaY"
                     /locus_tag="AC16FV_05590"
     CDS             complement(2884..3198)
                     /gene="cyaY"
                     /locus_tag="AC16FV_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HIR2849567.1"
                     /translation="MNESEFDKKATDTLLRIEQAVEESGADIDFEAAGGILTLEFGNG
                     SKIIINKQGAAKQIWVAAKSGGFHYGYVNGQWINDQGGGELMSELSRLVREQSGEDVD
                     LG"
BASE COUNT          696 a         1012 c         1051 g          616 t
ORIGIN      
        1 gctagtttta tctgaaacat gacttgccgg cagccggtcc cggttgccgg cgggacctgt
       61 ccggcaggct ggataagatc gtcaccagcc actccaccct tctcggaccc ggcgccgacg
      121 ggacatactt gaatcattgc ggcccgaccc caatgatgaa gccatggcca accgggaaga
      181 actgctaaaa acggaagctg aagacgccgc cgcaaccccc gcggttgtcg agagcgaggg
      241 cggcaagctc gcgcgcccag gcgatgtatt gccgggactg ctgcatatcc tgccgctcgc
      301 caatcgtccg ttcttccccg cgcaactggt gccgctggtg gtggagcgcg acccgtggca
      361 acgcacgctg gaagcggcgg tcgaggccgg ccagtacgtc ggtctggtgc tgatccgcgg
      421 cgacgacccg caaaccgccg gacccgacga tttctacacc atgggtacgg ccagccgcat
      481 tcataaagtg gtgcagctgg atgacaaact gcaactgttc gtcgagggcc tgcaacgttt
      541 ccgcatcgaa tcgtgggtga cgcgcgagcg cccgttcgcg gtgcgcgccg aatattttcc
      601 cgaaaccagc tacaaggaca tgtcggagct caaggcctac acggtggcgg tcatcaacac
      661 catcaaggag ttgctgccgc tcaatccgtt gtacggcgag gagctgaaat attttttaaa
      721 ccgcttcgga ccagatgacc cggcgcggct ggccgatttc gccgccagtc tcacgacttc
      781 cgacaagcag gaactccagg aggtactgga aaagacccac atccgcgagc gcatggaaaa
      841 ggtgctggtg ctgctcaaga aggaagtcga ggtggccaag gcgcagatgc agatccacaa
      901 gaacgtggaa gagagcatgg agaagcgcca gcgcgagttc ctgctgcgcg agcagatgaa
      961 ggcgatccag aaagagctcg gcatcgccaa ggacgaccgc accgccgagc tcgacaagtt
     1021 ccgcgcgcgc gtcgaggatt tgacgctgcc cgaggaagcg cggaaacgca tcgatgagga
     1081 aatcgagaaa atgtcggtgc tcgaggtcgg ttcgccggaa tacggcgtga cgcgcaatta
     1141 cctcgactgg ctgacgcaac tcccgtgggg aaaatattcg aaggataatc tcgacctcaa
     1201 gaatgcgcgc aagattctgg atcgcgacca cgacggtctc gtggacgtca aggatcgcat
     1261 tattgaattt ctcgccgtcg gcgccatgaa gggcgagatc gcgggctcca tcctgctgct
     1321 ggtcggtccg cccggcgtgg gaaagacttc catcggccgt tccatcgccg ccgcgctggg
     1381 ccgcaagttt taccgcttct cgctcggtgg catgcgcgac gaggccgaga tcaagggcca
     1441 ccggcgcacc tacatcggcg ccatgccggg caaattcatc caggcgatca aggacgtcgg
     1501 cgtggccaat ccggtgatca tgctggacga gatcgacaag atcggcgcct cctaccaggg
     1561 cgacccggcc tcggcgctgc tggaggtgct ggacccggag cagaaccgcg atttcctgga
     1621 tcattatctg gacgtgcgtt tcaacctctc caaggtgttg ttcgtgtcca ccgccaacca
     1681 gcttgacacc attcccgcgc ccctgctcga ccgcacggaa atcatccgtc tttccggcta
     1741 catcacgagt gagaaaatgg cgatcgcaca gcaccacctg attccgcgcc agttagagag
     1801 ggcgggcctg aagatggggc agctgcggat tgaaagcgat gccgtgcgcg agatcgtcga
     1861 gcgctacgcg cgcgaggccg gcgtgcgcaa tctggaaaag cgtctcggcc agatcgcgcg
     1921 caagagcgtg gtgaagattc tgaatggcgc caagccgccg atccgcgtgg gcgtgaagaa
     1981 tatcgaggag tatctgggca agccgctgtt caggcccgaa aaaccccagt atggcgtggg
     2041 tgtgatcacg ggtctggcgt ggacgccgct gggcggggcg acgctggatg tcgaggccac
     2101 gcgcgtgcac caggcgacgc gcggtttcaa gctcaccggc cagctcggcg atgtgatgaa
     2161 ggaatcagcc gagatcgcct acagctatat ctcctcgcac tgcaaggatt acggtgccaa
     2221 agcgaattat ttcgagaagg ccttcgtgca cctgcacgtg ccggccggcg ctactccgaa
     2281 ggacggcccg agcgccggcg tcaccatggc gaccgcattg ctttcgctcg cgcgcggtaa
     2341 caggatcgcc cgcccggtcg ccatgacagg cgaacttacg cttaccggcc aggtgttgcc
     2401 ggtcggcggt attcgcgaga aggtgatcgc ggcgcggcgc gcgaagattt ccgagctgat
     2461 actgcccgag gcgaatcgcg gcgatttcga cgagctgccg gcgcacatcc gcaagggact
     2521 caaaacacat ttcgcgagcc attaccggga cgtggcgaag cttgtttttc cgagaaagaa
     2581 ataaaagggg ggagttatcc cgtgggacgt tttgtcagct tcaagtgcag gttttgccag
     2641 tacgaggagc ccagccttgg cgtcggccgc ggcaaagcag catttccatt cctggcgctg
     2701 tatcgctgca accactgcca taccatcggc agcacctgga tatatgaaaa caagatcccg
     2761 cgttgcagcc agtgttacga cgacgcgctc accctgctgc cggacgacac ccggcgtgtg
     2821 aactgcccca agtgcggcga accggcggtg attcagccga aggaaggatg ctgggagtga
     2881 acctcagccg agatcgacgt cttcgccaga ttgttcgcgc acgaggcgcg acagttcact
     2941 catgagttcc ccgccgccct gatcgttgat ccactggccg ttgacgtaac cataatgaaa
     3001 gccgccggat ttggcggcga cccagatctg tttcgccgca ccctgcttgt tgatgatgat
     3061 cttcgagcca ttaccgaatt cgagcgtgag gatgccgcca gcggcctcga aatcgatgtc
     3121 cgcgccgctt tcctcgacgg cctgctcgat gcgcaacagg gtgtcggtgg cctttttgtc
     3181 aaattcggat tcgttcatgg tgtatgttct gtgccgctgt cacccgcatg ttagcgaagg
     3241 cgatgaccgc tgaacaggcc gcccgatctg atatgatccc gcggcaatta tgggaaagcg
     3301 gattcttgtc ctgggagggt atggcacttt tggccggcac atcagtcggg ccatcgccgc
     3361 catgccatcc gtcga
//
LOCUS       DBNGZE010000216         7525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1419884, whole genome shotgun sequence.
ACCESSION   DBNGZE010000216 DBNGZE010000000
VERSION     DBNGZE010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1419884"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="AC16FV_05595"
     CDS             complement(<1..450)
                     /locus_tag="AC16FV_05595"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HIR2849568.1"
                     /translation="MITVEPVAGLREQIARLRQSGKRIAFVPTMGNLHAGHMRLMQEA
                     RRHAQAVVASIYVNPLQFGQGEDFDSYPRTPSHDKVVLLSAGVDVLFKPAESEIYPRG
                     KTAQTFVEVPGLSNELCGAFRPGHFRGVTTVVNRLFNLVTPDVAVFGK"
     gene            complement(447..1253)
                     /gene="panB"
                     /locus_tag="AC16FV_05600"
     CDS             complement(447..1253)
                     /gene="panB"
                     /locus_tag="AC16FV_05600"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR2849569.1"
                     /translation="MKPVTLSTIREMKRRGEKITCLTAYDYSFASLLDNAGIDMIMVG
                     DSLGMVMQGHDTTLPVSVADMAYHSRCVARGTKRALIIVDMPFMSYQQSPEHAFASAG
                     RLMQKGGAQVVKLEGGEPMAETVRFLVERGAPVCAHLGLTPQSVHQLGGYRVQGRETT
                     SAERIRHDARVLQEAGASLLVLEAVPSELAKTITSDLEIPTIGIGAGPDCDGQVLVLQ
                     DMLGIYPRPSPKFSKNFMRGAASIDEAVKAYIAAVKSGTFPGPEHSFHSS"
     gene            complement(1250..1900)
                     /locus_tag="AC16FV_05605"
     CDS             complement(1250..1900)
                     /locus_tag="AC16FV_05605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR2849570.1"
                     /translation="MNANNPGYVVVEGPIGVGKTSLAVRLAEVFGAKPLLEHPEENPF
                     LERFYQSRKSFALPTQLFFLFQRARQMEQIKQSDMFSSGYVSDFLLDKDKLFARANLD
                     DDELRLYEQVYAQMSLNLPVPDLVIYLQAPVEVLHERVRRRGVEYERLIDRDYLQILV
                     DAYTQFFHHYTAAPLLVVNAERINIVDREADFQTLLSHIRKIRSGRHFFNPLAANL"
     gene            complement(1897..2409)
                     /gene="folK"
                     /locus_tag="AC16FV_05610"
     CDS             complement(1897..2409)
                     /gene="folK"
                     /locus_tag="AC16FV_05610"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR2849571.1"
                     /translation="MSKPSTITVRAYIGLGSNLTDPVVQVRTGIAALGQLEQTQVETC
                     SSFYRTAPVGLREQPDFINAVCRLRTGQAPAALMQNLLEIEHAHGRVRHGDKGGPRTL
                     DLDLLLYGDRVIQTAELTVPHPRLHERAFVLYPLHEIEPDLVIPEHGALRELLAACTG
                     QRVEKLDNLL"
     gene            complement(2402..3793)
                     /gene="pcnB"
                     /locus_tag="AC16FV_05615"
     CDS             complement(2402..3793)
                     /gene="pcnB"
                     /locus_tag="AC16FV_05615"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR2849572.1"
                     /translation="MLGHCLRNDPGGIKINEPVIIPRAQHVITRAQISENALKVLHRL
                     KEAGYASLLVGGCVRDLMLGREPKDFDVVTDARPEQIRKLFHNARVIGRRFRLVHVRF
                     GWDIIEVATFRAIPRDISEEPLSTEEEAAESEDVEDSSPVEAPDHNVFGSQEEDAVRR
                     DFTVNALYYDVHDFSVIDYVGGVPDLKAGILRVIGDPETRYREDPVRMLRAVRFAAKL
                     GFKLEDKTAAPIRELASLLASVPPARMFEEVLKLFHGGYALETYELLRHYGLFQYLFP
                     LTEKSLEHEEEGFPVTLVPRALANTDARVNEDKPVTPAFLFAAMLWEPVREQMGEYIV
                     RGMNGHDAMFRAAEHVLREQLRHITIPKRFSVPMREIWSMQGRLERRAGQQAFRLLES
                     KRFRAAYDFLLLRAETGEADPALAEWWTRFQSVGENERRIMVAEFATPGGGGKRRRRR
                     RRRSSSQQAPRHE"
     gene            3873..3948
                     /locus_tag="AC16FV_05620"
     tRNA            3873..3948
                     /locus_tag="AC16FV_05620"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3907..3909,aa:Glu,seq:ctc)
     gene            complement(4003..4671)
                     /locus_tag="AC16FV_05625"
     CDS             complement(4003..4671)
                     /locus_tag="AC16FV_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR2849573.1"
                     /translation="MVILKESPRDWGLVAVVVLLSIGANLPEEWTNWIDLDRRYLLGG
                     LIAVIAVALVRYLKFTLILVIVVLAAGANVPADIAREFGIDPQISLLGLVAMIVVSLS
                     NRLLKLPTGLEKSGRSKTAHGAAALFTAILKGRIAVVQSLLNQGVNVNVRTVSGKTPL
                     MAAAYKGYSDIVQMLLDNGADVTSKDGRGDTAEKIAERGGYTRIVELLKKGSSTSNVM
                     APAQ"
     gene            complement(4859..5584)
                     /locus_tag="AC16FV_05630"
     CDS             complement(4859..5584)
                     /locus_tag="AC16FV_05630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2849574.1"
                     /translation="MHGWLRLSRGRLRGMDIRPREEAVVEITTGTQPMASIIWLHGLG
                     ADGHDFEAIVPELRLPQEPVLRFVFPHAPYRPVTINGGYVMRAWYDIAMSEHGFEQNI
                     EHIRESEKILQALIENEIRRGISCERIVLAGFSQGGAIALYTGLRYPKPLAGIMSLSA
                     PVPYVESLMAEISPANTAVPVFMAHGTDDRMIPFNLAQQAHERLEVHGLHLERHTYVM
                     GHTVVPDEIRDIARWLARVLGNK"
     gene            complement(5548..5907)
                     /gene="queD"
                     /locus_tag="AC16FV_05635"
     CDS             complement(5548..5907)
                     /gene="queD"
                     /locus_tag="AC16FV_05635"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HIR2849575.1"
                     /translation="MEIFKIFNIEAAHHLPNVPENHKCRRLHGHSFRIEIHVAGPVQD
                     QSGWIMDFADLSRAFQPLYEQLDHHYLNEIEGLQNPTSEHLARWIWRKLKPALPALSK
                     VVVRETCTAGCVYRGED"
     gene            6178..6993
                     /locus_tag="AC16FV_05640"
     CDS             6178..6993
                     /locus_tag="AC16FV_05640"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR2849576.1"
                     /translation="MTQPLLPLIVEPAELEKNLGADNLLIVDLCRPETYAQAHLPGAA
                     HLDYAQIIAARPPAMGMLPDAARLSDVLGSIGMKPDNHVVAYDDEGGGRASRLLWTLD
                     VIGHSRYSLLNGGLHAWLNDRHPVSNETIKRTRSDYTVRFDDKVVADKNYIMNHLRDT
                     GVVIVDTRTPAEYRGETKRAERSGHIPGAVNFDWMNAIDQTRSVRLKTAEELKRVLTG
                     LGVTPDKEVITYCQTHHRSAHTYIVMKSLGYPRVRGYPGSWSEWGNSPETPIE"
     gene            complement(6983..7444)
                     /locus_tag="AC16FV_05645"
     CDS             complement(6983..7444)
                     /locus_tag="AC16FV_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849577.1"
                     /translation="MNQWRLENSGVADVIAGMNVYAISYTTTVHLNHAICRHGIFIRE
                     IICNSACFDNNPSSETKVAHELPFLSCKVRITGVGGGGEMKKWLNKIKSGMTALTSAK
                     TDDAAWQETAAMVYVGDIYAVLLRDRVARRLAQTDSRGSDYRRDASWPFIR"
BASE COUNT         1618 a         2268 c         2134 g         1505 t
ORIGIN      
        1 cttgccgaac acagcgacat cgggcgtcac taggttgaac aaacggttca ccaccgtggt
       61 gacgccgcga aaatgcccgg gacggaaagc gccgcaaagc tcattgctca agcccggcac
      121 ttccacgaag gtctgggcgg ttttcccgcg cggatatatc tcggactccg ccggcttgaa
      181 cagcacgtca acaccggcgg aaagcaggac caccttgtcg tgcgacggcg tacgcggata
      241 ggaatcgaag tcctcgccct gcccgaactg caggggattg acgtaaatgc tcgccaccac
      301 cgcctgcgca tgacggcgcg cctcctgcat caggcgcata tggccggcat gaagattgcc
      361 catggtcgga acgaaggcga tgcgcttgcc gctttggcgc agccgcgcga tctgttctct
      421 caacccggca acgggttcaa ccgtaatcat gaggaatgaa agctgtgttc cggaccgggg
      481 aaggtgccgg atttgaccgc agcgatgtac gcctttaccg cttcatcgat actggcggcg
      541 ccccgcatga aatttttcga aaacttgggg ctcggccgcg gatagatacc gagcatgtcc
      601 tgcagcacca gcacctggcc gtcacagtcc gggccagcgc cgataccgat cgtggggatt
      661 tcgagatcgg aggtaatcgt cttcgccagt tccgacggta ccgcttcaag cacaagcagc
      721 gacgcgccgg cttcctgtaa aactctggcg tcgtgacgaa tccgttccgc ggaggtagtt
      781 tcacgtccct gcacgcgata accacccagc tggtgcaccg attgaggcgt caaccccaga
      841 tgcgcgcaca cgggagcacc gcgttcgacc agaaaccgga ctgtctcggc catgggttca
      901 ccgccctcca gcttcaccac ctgcgcgccg cccttttgca tcaatcgtcc agccgaggca
      961 aaggcgtgtt ccgggctctg ctgataactc atgaaaggca tgtcgacaat aatcagcgcg
     1021 cgttttgtcc cgcgcgccac gcagcggctg tgatacgcca tgtcggcgac gctgaccggg
     1081 agcgtggtgt cgtgaccctg catgaccatg ccgagggaat cccccaccat gatcatgtcg
     1141 atgccggcgt tgtcgagcag gctggcgaag ctgtagtcgt aggcggtcag gcaggtgatt
     1201 ttctcgccgc ggcgcttcat ctccctgatc gtgctgagcg tgaccggctt cacaggttgg
     1261 cggcgagagg attgaagaaa tgacggccac tgcggatctt gcggatatgg gagagcaaag
     1321 tctgaaaatc cgcctcgcgg tccacgatgt tgatccgctc ggcattgacg acgagcaagg
     1381 gcgcagccgt gtagtgatga aaaaactggg tataggcatc caccaagatt tgcaggtaat
     1441 cgcgatcgat aagccgttcg tattccacgc cgcgacggcg cacgcgctcg tgcagcacct
     1501 ccaccggcgc ctgcagatag atcaccagat ccggtaccgg cagattcaga ctcatctggg
     1561 catacacctg ttcatacagc cgcagctcgt cgtcatccag attggcgcgc gcgaacagct
     1621 tgtccttgtc gagcaggaaa tccgagacat aaccgctgct gaacatgtcg ctctgcttga
     1681 tctgctccat ctggcgcgcg cgctggaaca ggaagaacag ctgggtcggc aacgcaaacg
     1741 atttacgtga ctggtagaac cgttcgagaa aagggttctc ttccgggtgt tcgagcaagg
     1801 gcttggcgcc gaaaacctcc gccaggcgca cggcgagact ggtcttcccc acaccgatcg
     1861 gcccttcgac aacgacatac cctggattgt tggcgttcat aataaattgt ccagcttctc
     1921 tacccgttgt ccggtacagg ctgccagcag ttcgcgcaat gcgccgtgct ccggaatgac
     1981 cagatccggc tcgatttcgt gcaaaggata caggacgaag gcgcgctcgt ggagtctcgg
     2041 atgcggcacg gtcagttccg ccgtctggat cacccgatcg ccgtacaaca acaggtcgag
     2101 atccagcgtg cgcggaccgc ctttgtcccc atgccgcacg cgtccgtgcg catgttcgat
     2161 ttcaagcagg ttctgcataa gcgccgctgg cgcctgaccg gtgcgtaaac gacagacagc
     2221 attgatgaag tccggttgtt ctcgtagtcc caccggtgca gtgcgataaa acgacgagca
     2281 tgtctcgacc tgagtctgct caagctgtcc cagcgccgca atcccggtgc gcacttgtac
     2341 gacgggatca gtcaggttac tccccagccc gatgtaagcg cgtaccgtaa ttgtcgaagg
     2401 cttactcatg cctcggcgcc tgctgggacg acgatcggcg ccggcggcgg cgacgccgtt
     2461 tgcccccacc gccgggcgtg gcgaattccg cgaccatgat gcggcgttca ttctcgccca
     2521 cagactgaaa acgcgtccac cactcggcca aggccggatc ggcctcgccg gtttcggcgc
     2581 gcagcaacag aaagtcataa gcggcgcgaa agcgtttgct ctcgagcaga cggaaggcct
     2641 gctggccggc gcggcgctca aggcggccct gcatgctcca tatttcacgc atcggcacac
     2701 tgaagcgttt cgggatggtg atgtgtcgca actgctcgcg caatacatgt tccgcggcgc
     2761 ggaacatggc gtcatggccg ttcatgccgc gcacgatgta ttcacccatc tgctcccgca
     2821 ccggttccca cagcatggcg gcaaaaagaa acgccggcgt caccggcttg tcctcattca
     2881 cgcgcgcatc cgtattcgcc aacgcacgcg gtaccagtgt cacggggaaa ccctcttcct
     2941 cgtgttcgag acttttttcc gtcagcggga acagatactg gaatagaccg tagtggcgca
     3001 gtaattcgta ggtttcgagc gcataaccgc cgtgaaacag cttcaatacc tcttcaaaca
     3061 tgcgtgccgg cggtacggaa gccagcaaag acgccagttc gcggatgggt gcggcagtct
     3121 tgtcctcgag tttgaaaccc agcttggcgg cgaagcgcac cgcgcgcaac atgcgcaccg
     3181 ggtcctcgcg atagcgcgtc tcgggatcgc caattacgcg cagaatgccg gccttgagat
     3241 cgggtacgcc cccgacataa tcgatgacac tgaaatcatg aacgtcgtag tacagcgcgt
     3301 tgacggtgaa atcgcgccga accgcgtctt cttcctggga accgaacacg ttgtgatccg
     3361 gcgcctccac cggagaactg tcttcgacat cctcgctttc tgccgcttct tcttccgtcg
     3421 aaagtggttc ctccgaaatg tcgcggggaa tagcgcggaa ggtggcgact tcgatgatgt
     3481 cccatccgaa acgcacgtgc accaggcgga aacgccggcc gatcacgcgt gcgttgtgga
     3541 acagcttgcg gatttgctcg ggtcgtgcgt cggtcacgac gtcgaaatcc tttggctcgc
     3601 gtcccagcat gagatcgcgc acgcatcctc ccaccagcag gctggcgtag ccggcttctt
     3661 tcaggcggtg caggaccttg agggcgtttt cgctgatttg agcgcgcgtg atgacgtgtt
     3721 gcgcgcgcgg aatgatgacc ggttcgttaa tctttattcc acctgggtcg ttacgaagac
     3781 aatggccgag gatgccgtat tatcagttgg tgctttgtca gccggcacgt ataataccat
     3841 cgcttttagc gccacgcgcg ctatctccca acgctcccat cgtctagtgg cctaggacgc
     3901 tggcctctca cgccggtaac aggggttcga atccccttgg gagcgccaaa acttaagagg
     3961 accctttatg ggtcctcttc gtttttcagg caacgcgccg gcttattgtg ccggcgccat
     4021 cacgttgctc gtactactac cttttttcaa caactccacg atacgcgtat acccgccgcg
     4081 ctcggcgatc ttttccgccg tgtcgccacg tccatccttg gaggtcacgt ccgcgccgtt
     4141 atcgagcagc atctggacaa tatcgctgta acctttatag gccgccgcca tcaacggcgt
     4201 cttgccactg acggtgcgca cgttcacatt cacgccctgg ttcaacaatg actgcaccac
     4261 ggcgatgcgc cctttgagga tggcggtgaa cagcgccgcc gcgccgtgcg ctgttttgga
     4321 acggccgctc ttctcgagac cggtcggcag cttgagcagg cggttgctga gcgaaaccac
     4381 gatcatggcc accagtccaa gcaggctgat ctgtgggtcg atgccgaatt ccctggcgat
     4441 atccgcgggc acgttggcgc cggcggcgag aaccacgatg accaggataa gagtaaattt
     4501 aaggtagcga accagcgcca cggcaataac cgcgatcaga ccgccaagca aatagcggcg
     4561 atccaaatcg atccaattcg tccactcttc gggaaggttg gcgccgatac taagcaacac
     4621 gacgacggca acgagtcccc agtcccgtgg cgattctttt aatatgacca caatacctct
     4681 cctctgtttt ctatcaggtg atcaccgcca ccagcacccc tgtcgcggta catagcctta
     4741 agtctagatg catttttcgc cctgtgaagc cggcagaaat gcaaaaaact ggcgcagaat
     4801 agcggaaaac tccggacatt ccgggcaaaa ctgccagcaa ccggtttatc cacccttgtt
     4861 acttgttacc gagaacacgc gccagccaac gggcaatgtc ccgaatttcg tccggcacca
     4921 cagtatgccc cataacataa gtatgccgct caagatgaag tccgtgcacc tccagccgct
     4981 catgcgcctg ctgcgccagg ttaaatggaa tcatgcgatc gtctgtgcca tgcgccatga
     5041 aaaccggcac ggcggtgttg gccggactga tttcagccat caggctctct acataaggca
     5101 caggcgcgga caaagacatg atccccgcga gcggcttggg ataacgcagg ccggtataca
     5161 acgcgatggc gccgccctgg gaaaatccgg caagcacgat acgctcgcag gagatgccgc
     5221 gccggatttc gttctcgatc aaggcttgca ggattttctc ggattcgcga atatgttcga
     5281 tgttctgctc aaaaccatgc tcagacatgg cgatgtcata ccaggcacgc atgacataac
     5341 cgccattgat agttaccggc cgatagggcg catgcggaaa cacgaagcgc aacacgggct
     5401 cctgcggcaa ccgcagctcg ggaacgatgg cttcaaaatc gtggccgtcc gcgcccaatc
     5461 catgcaacca gataatactg gccattggtt gagttcctgt tgtgatctca acaacagctt
     5521 cttcgcgcgg ccggatatcc atgcccctca gtcttccccg cgatagacgc agccagccgt
     5581 gcaagtctca cgtacgacga ccttggaaag cgccggtagt gccggcttga gcttgcgcca
     5641 gatccagcgg gcaagatgtt cgctggtggg gttttgcagg ccctcaatct cgttcagata
     5701 atgatgatcg agctgctcgt aaagcggctg gaaagcgcgg gacagatcgg caaagtccat
     5761 gatccagccg gactgatcct gcaccggtcc tgcgacgtga atctcgatcc ggaatgaatg
     5821 gccatgcaga cgccggcatt tatgattttc cggcacatta ggcaggtgat gcgccgcttc
     5881 gatgttaaaa atcttgaata tttccacggg aataaccgga cgaaaaaaat gaatacacaa
     5941 gtttagcggc tttcgcgtta cccggaaacc gcggcccatg agaagtggtg caggggtgca
     6001 gggaggcacc gcttacggag ccaaggacgc aatgggccgt ctgcggtgcc gagggacgca
     6061 ggagtggtac agggaggtac cgtctacgaa gctaaggacg caaaaccatg catcgcactc
     6121 gcggtgctgc cgacaggggg ctagaataat ccagcgttcc aatccccccg aggagtcatg
     6181 acccaacctc ttctgccgct aatcgtggaa ccggcggagc tggaaaagaa tcttggcgcg
     6241 gacaatcttc tgatcgtgga tttgtgccgg cccgaaacct acgcgcaggc ccatctgccc
     6301 ggcgcggcgc atctcgatta cgcgcaaatc atcgccgcgc gcccacctgc catgggcatg
     6361 ctgccggatg ccgcgcgcct cagcgatgtg ctcggttcca ttggcatgaa accggacaac
     6421 catgtcgtgg cctatgacga cgaaggtggc gggcgcgcct cacgcctgct ctggacgctg
     6481 gatgtcatcg gacattcgcg ctattcactg ctgaacggcg gcctgcacgc ctggctcaat
     6541 gaccggcacc ccgtcagcaa cgagactatc aaaagaacaa ggagcgatta cacggtccgg
     6601 ttcgacgaca aggtcgtggc ggacaagaac tacatcatga atcatctgcg cgataccggc
     6661 gtggtcatcg tggacacgcg cacgccggcg gaataccgtg gcgaaaccaa acgcgcggaa
     6721 cgcagcggac atatccccgg agcggtgaat ttcgactgga tgaacgccat cgaccagacg
     6781 cgcagcgtgc gcctgaaaac agcggaagaa ctaaagcggg ttctgaccgg ccttggtgtg
     6841 acgccagaca aggaggtgat tacgtattgc cagacgcatc accgttcggc gcatacctat
     6901 atagttatga aaagcctggg ctatccgcgg gtacgcggct atcccggctc atggtcagaa
     6961 tggggcaaca gccccgaaac gcctatcgaa tgaagggcca gctggcgtcg cggcgatagt
     7021 cgctaccacg gctgtcagtc tgtgccaaac ggcgagcaac acggtcgcgc aacagcacgg
     7081 catagatatc cccgacgtaa accattgccg ctgtttcctg ccacgcggcg tcatccgtct
     7141 ttgcggatgt cagcgccgtc attcccgact tgatcttgtt caaccacttt ttcatttctc
     7201 cccctccccc caccccggtg atccggacct tgcatgatag gaatggcaat tcgtgtgcca
     7261 ctttcgtttc ggaagagggg ttattatcga aacacgctga attacaaatt atttccctga
     7321 taaaaatccc gtgacggcat attgcatggt tgagatgaac ggtcgttgtg taagaaatgg
     7381 catacacatt catgccggcg ataacatcgg ccacgccgga attttccagt ctccattgat
     7441 tcatgaagga tcttgccgca agcaatcaag gatttagccc ctatgctcaa aatggaggca
     7501 tagctttcgc tacgtttggc ggatg
//
LOCUS       DBNGZE010000217         3875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_446948, whole genome shotgun sequence.
ACCESSION   DBNGZE010000217 DBNGZE010000000
VERSION     DBNGZE010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_446948"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="AC16FV_05650"
     CDS             <1..1422
                     /locus_tag="AC16FV_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849578.1"
                     /translation="PVSQLSLEFTKQYIGLPLPLSEKHKSIAEQNRQFQMEMANGYKR
                     IVLGISSPSTHDTQEHAFRALVIERAIRYLTGALAVSYQTYSPCPEGAWKEIHALYAH
                     AENLGLAEIEVADPFNKAISACSVSHAYKQALLLDFSDPYHLPPRMIDWTHHYLDRWA
                     PLAQLAAATSVYHPTCQFLIDQHNDHAGIAYTTGTVPEDPKRYRLLNTVELARQVHAQ
                     LTQLINGEKPSAEGLQENFFQECGQDLLRRLVNAWGVNPQRSFRRSHAINRQAEIAVG
                     MDRINYWINGGKKFVVSSTFVGPVPQRTMLASEEIKHKDTRIPGRELSTWDVEDEGAG
                     GLALSKSGQIHLHIQVGDLLVTRTPGDGNPWSVGVIRWVRNTGSSNLEIGIQHLAPRA
                     DPVVIKTVNESGKESDFLPALLLPEIKPLKQPQTLITHRGVFRPENGVFMDNGYRLYR
                     IAPARLVEASHSFEQFSFDILNT"
     gene            complement(1429..2283)
                     /locus_tag="AC16FV_05655"
     CDS             complement(1429..2283)
                     /locus_tag="AC16FV_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR2849579.1"
                     /translation="MNFLSAFLRLSRWVVQRFREDRCTRVAGALSFTTLLAIVPLTAV
                     TFAIFSRFEIFESWMTMVQEFIYGNFVPASGEAVSGYLRKFAANAGKLTIWGLLFLFL
                     TSLMLMATIERVFNDIWHVPHTRKRLHRYLGYGALLVLGPALMGISLASTSYLISLPL
                     FARHAPLGDVKVLLLAAAPVIFEWLAFWALYVVVPNYHVRLRHGLIGSLIATVAFEIA
                     KRGFAFFVTHFANYKAIYGAVAALPVFLIWVYLSWTIILAGAVVTATLPEWRRPQAVR
                     QWRRKRPT"
     gene            2384..2731
                     /gene="arsC"
                     /locus_tag="AC16FV_05660"
     CDS             2384..2731
                     /gene="arsC"
                     /locus_tag="AC16FV_05660"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366186.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR2849580.1"
                     /translation="MAVTIYHNPRCSKSRQTLALLEKRGIEPKIIEYLVTPPTETELK
                     HLLKLLGISPRELLRTKEEEYKKAKLDKPDVSDAEIIRAIAKYPRLMERPIVVAGNKA
                     ALGRPPESILKIL"
     gene            complement(2728..3465)
                     /gene="hda"
                     /locus_tag="AC16FV_05665"
     CDS             complement(2728..3465)
                     /gene="hda"
                     /locus_tag="AC16FV_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HIR2849581.1"
                     /translation="MSHQLSLNLRLKDASSFGNFLPGPNREALEQLRASVVKAATQAN
                     NATASMLYLWGAEGSGKTHLLQAACRLAQELGIAPVYVPLADVVALEPSLLEGVEAMP
                     LVCLDDAERAAGRAEWEAALFSLVERMRSVGGMLAIAASAPADRLGLRLPDLASRLAW
                     GTVYALQPLDDAQKLEAVRLRARHRGFEMPEDVARYILSRYPRDLRSLFDLLDRIDQA
                     SLAQQRRVTIPFLRRLEELGHKSVETA"
     gene            complement(3462..>3875)
                     /locus_tag="AC16FV_05670"
     CDS             complement(3462..>3875)
                     /locus_tag="AC16FV_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR2849582.1"
                     /translation="LVGLDLALPIGIVAGLLSFVPYLGFITGLLSAAVAAYVQFQDVI
                     MLLGVAIVFLVGQALEGLWLTPRLVGNRIGLHPVAVIFAVMAGGRLFGFTGILLALPV
                     AAVLKVWLRHLHEFYISGAANQPVKKRRALRRPPP"
BASE COUNT          902 a         1265 c         1093 g          615 t
ORIGIN      
        1 ccggtcagtc agctttcact ggagttcacc aagcaataca tcggtctgcc gttgccgctt
       61 tccgagaagc acaagagcat cgccgagcag aatcgccagt tccagatgga gatggcaaat
      121 gggtacaagc gcatcgtgct gggcatatcc tccccgtcca cgcacgacac ccaggaacat
      181 gcattccggg ccctcgtgat cgaacgcgcg attcgctacc tgaccggcgc gctcgcggtt
      241 tcctatcaga cctactcacc ctgccccgag ggcgcgtgga aggaaattca cgcactctat
      301 gcgcatgcgg agaatctcgg gctggccgag atcgaggttg ccgatccctt taacaaggca
      361 atatcggcgt gcagcgtaag ccacgcctac aaacaagcgc tgctgctcga cttcagcgat
      421 ccttaccacc tgccgccgcg catgatcgac tggacgcatc actatctcga tcgctgggcg
      481 ccgctggcac aactggccgc ggccaccagc gtctaccatc ccacctgcca gttcctgatc
      541 gatcaacaca atgaccatgc gggcatcgcc tacaccaccg gtaccgttcc ggaagatccg
      601 aagcgttacc ggctcctgaa cacggtcgag ctggcgcgac aggtgcatgc gcagcttact
      661 caactcatca acggcgaaaa gccgtcagcc gaaggattgc aggaaaattt ctttcaggaa
      721 tgcggccagg acctgttgcg gcggctggtc aacgcctggg gcgtcaatcc gcagcgatcc
      781 ttccgccgca gtcatgcaat caatcgccag gcggaaatcg ccgtcggcat ggaccggatc
      841 aattactgga tcaacggcgg gaaaaaattc gttgtcagct cgaccttcgt cggtcccgtg
      901 ccacaacgta ccatgcttgc ctccgaggaa atcaaacaca aagacacacg catccccggg
      961 cgcgaattgt ccacgtggga cgtcgaggac gaaggcgccg gcggactcgc gctttcgaaa
     1021 agcggtcaga tccatctgca tatccaggtc ggcgacctgc tcgtcacgcg cacgccggga
     1081 gacggcaacc cctggagcgt gggcgtcatc cgctgggttc gcaacacggg ctcgtcgaat
     1141 ctcgagatcg gcatccagca cctcgccccg cgtgccgatc cggtggtcat caaaaccgtg
     1201 aatgagagcg gaaaggagag cgactttctc ccagcactgc tgttgccgga aatcaaaccg
     1261 ctcaaacaac cgcaaacgct gatcacccat cgcggcgtgt tcaggccgga aaacggggta
     1321 ttcatggaca acggttaccg tctgtaccgg atcgcgcctg cccggctcgt cgaggccagt
     1381 cactcgttcg agcaattcag cttcgacatc ctgaatacct gatcaacctc atgttgggcg
     1441 cttgcggcgc cactggcgta cggcctgcgg gcgtcgccac tcgggcaggg tcgccgtcac
     1501 caccgccccc gccaggatga tggtccacga cagataaacc cagatcagaa acaccggcag
     1561 cgccgccacc gccccgtaga tcgccttgta gttcgcgaag tgcgtcacga aaaacgcgaa
     1621 accgcgcttg gcgatctcga aagccaccgt ggcaatcagg cttccgatca gcccatgacg
     1681 cagccgcacg tgataattcg gcaccacgac gtacagtgcc cagaacgcca gccattcgaa
     1741 aatcaccggc gccgccgcca gcaataacac cttcacgtcg ccgagcggcg catggcgcgc
     1801 gaacagcggc agggaaatga gataagaggt ggacgccagg ctgatgccca tcagcgccgg
     1861 acccagcacc agcaaggcgc cgtaaccgag ataacggtgc aggcgcttgc gcgtatgcgg
     1921 cacgtgccag atgtcgttga acacgcgctc gatcgtggcc atgagcatga gcgaggtgag
     1981 gaacaggaac agcaggcccc agatcgtgag cttgcccgcg ttggcggcaa acttccgcag
     2041 atagccgctg actgcctcgc ccgaggccgg aacaaaattg ccgtagatga actcctgcac
     2101 cattgtcatc cacgactcga atatctcgaa acgggaaaaa atcgcgaacg tcacggccgt
     2161 caacggcacg atcgccagca gcgtcgtgaa cgacagcgcc ccggccacgc gcgtgcagcg
     2221 gtcttcccgg aaccgttgca ccacccagcg cgacaaccgc agaaatgccg ataaaaagtt
     2281 catgtcccta tgttagtcga attacggcca ccggcacttg ctggcggcgg ccacccgtat
     2341 caaaatcgga accgcatcgc cgccgaaaaa cccgggagaa tccatggccg tcacgattta
     2401 ccacaacccc cgctgctcga aatcgcgtca gacgctggcg ctgctggaga aacgcggcat
     2461 cgaaccgaaa atcatcgaat atctcgtcac cccgccaacg gagacggaac tcaagcatct
     2521 gctcaaactt ctcgggatat cgccacgcga attattgcgc acaaaagaag aggaatacaa
     2581 aaaggcgaaa cttgataagc cggatgtttc cgatgccgag atcatccgcg ccatcgccaa
     2641 atacccgcgc ctgatggagc ggcccatcgt ggtagcgggg aacaaggcgg cactggggcg
     2701 gccgccggag agtattctga agattctcta ggcagtttcc acgctcttgt gacccaactc
     2761 ttccagccgg cgtaaaaagg gaatggtcac gcgccgctgt tgcgccagcg aggcctgatc
     2821 gatgcgatcg agcaaatcaa agagtgagcg cagatcgcgc ggataacggc tcaaaatata
     2881 ccgcgccacg tcctccggca tctcgaaacc gcggtgccgc gcgcgcagac gcacggcctc
     2941 cagtttttgc gcatcatcca ggggctgcaa ggcgtaaacc gtgccccagg cgagacggct
     3001 cgcaagatcg ggaagtcgca aaccaagacg gtccgccggc gcggatgcgg caatcgccag
     3061 catgccaccg acactgcgca tgcgctcgac cagactgaac aatgccgctt cccattcggc
     3121 acgacccgcg gcgcgttccg cgtcgtcgag acacaccagg ggcatggctt caacaccctc
     3181 cagcaatgac ggctccagcg ccacgacatc ggccagcggc acatacaccg gcgcgatgcc
     3241 gagttcctgc gccagacgac aggccgcctg taataaatgg gtcttgcccg aaccttcggc
     3301 gccccacaga tacagcatcg aggccgtggc attatttgcc tgcgttgcgg ccttcaccac
     3361 ggaagcacgc aattgctcca gcgcttcccg attgggaccg ggaaggaaat tgccgaagga
     3421 tgacgcatcc ttcaggcgca gattgagcga gagctgatgg ctcatggcgg aggccgccgg
     3481 agcgcgcggc gttttttgac gggctggttg gcggcgccac taatatagaa ttcgtgcagg
     3541 tgccgcaacc agaccttcag caccgccgcc accggcaatg ccagcagaat gccggtgaat
     3601 ccaaaaagcc gcccacccgc catgacggcg aagatcaccg ccaccggatg cagcccgatg
     3661 cgattgccta cgagcctcgg cgtgagccac aagccttcca gcgcctgacc gacgagaaac
     3721 acgatggcca cgcccagcaa catgatgacg tcctggaatt gcacataggc cgcgacggcc
     3781 gccgacaaca ggccagtgat gaagccaaga taaggcacga aactcaagag acccgccacg
     3841 atgccgatgg gtagcgccag gtccaggccc accag
//
LOCUS       DBNGZE010000218         2127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1142058, whole genome shotgun sequence.
ACCESSION   DBNGZE010000218 DBNGZE010000000
VERSION     DBNGZE010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1142058"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..936)
                     /locus_tag="AC16FV_05675"
                     /pseudo
     CDS             complement(55..936)
                     /locus_tag="AC16FV_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            complement(999..1823)
                     /locus_tag="AC16FV_05680"
     CDS             complement(999..1823)
                     /locus_tag="AC16FV_05680"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HIR2849583.1"
                     /translation="MNIQAPLRLEQSSRPLEGRVSLITGSTSGIGLGIARALAAAGSS
                     VMLNGFGKPGDIAETQAKIVSEFGVKVRYSPADMSKAAPIADMIAMALDTFGRLDVLV
                     NNAGIQHISPLEQFPVEKWDAILAINLSSAFHTTRAALPSMRQNKFGRIINIASAHGL
                     IASPFKAAYVAAKHGIVGLTKVTALETAEANITCNAICPGYVYTPLVEAQIDGQAKSH
                     GIPREQVIRDVLLAQQPNKRFATVEELGALSVFFASDAAASITGTALPVDGGWTAH"
BASE COUNT          419 a          583 c          658 g          467 t
ORIGIN      
        1 gaaagcggcg agcgcttgca cttgttgtgt cctgcggact tcttccggca gctacttggc
       61 gaggtcacga atgacatgcc gcaggtggtg catctgacgt tgtcgtgcga tgtggcttgc
      121 gacgcggctg gaatactgga tgtccttgtg acgattgagg acctcccaaa tagtacgtgg
      181 ctcatcaccc attgggttcc acaagtggac ggcaaagatc agtgagttct tgcgtggatt
      241 gtcgtcgaag atcacttcgg ttggcgtgtt cgaaaggata ccaccgtccc aataaagctc
      301 accctcgatg cgaacggcag gaaaggccgg cggcaacgcg cccgacgcca taatgtgctt
      361 gacgccgatc tcgccgtcgc ggctatcaaa atagcgcatc tcgctggtac gaacgtgagc
      421 agcgccaacg gtgagtcgcg gattgcactt gttcaccagc gagaagtcga cgagttcgag
      481 cagtgtcttt tccagcggag cggtggagta gaagccggct gcgtcgggcc caaggggaac
      541 gtgcgtcccg aggaatgccc cagcattagg ctcgaaaaaa tccggaatac cgcgaagaag
      601 cgtcttccag taggagatca tgtcaggcaa tccggtccac gccggaacgg cgaacggcgg
      661 ccgatacgcc atccgcgtcc aaaattcttt tagcgtttcg aggcgatttc cgggtggatt
      721 gccggcgatc aggcttgcat tgatcgcgcc gatcgacgtg ccgacgatcc agtccggttc
      781 aatgccgtgt tcgtgcagtg cgtgataaac gccggcttgg taggcgccga gcgcccctcc
      841 gccttgcaga accaagacga cctggcgatc gttttgtttt tgagtctgtt gcgtatccgc
      901 gagcttcgcc tcactttttg atgaattctt ttccatgatt tcctccctaa aatcctgatt
      961 gctgacttgg gcttgcctag gcggggatct ccttcgcctt aatgcgcggt ccagccgcca
     1021 tcaaccggca gcgccgttcc ggtaatcgag gccgcggcat cacttgcgaa aaacaccgaa
     1081 agagcaccga gttcctcgac tgtggcgaaa cgtttgttcg gttgttgggc gagcagcacg
     1141 tcacgaatga cctgctcgcg cgggatgccg tgcgatttgg cctgaccatc tatctgcgct
     1201 tcgacgagcg gcgtgtagac atagccagga cagatggcat tgcaggtgat gtttgcttcg
     1261 gctgtttcca acgcagtgac tttggtcagg ccgacgatac cgtgcttggc tgccacatag
     1321 gctgccttga atggcgatgc gatgagcccg tgggccgaag cgatattgat gatgcggccg
     1381 aatttgttct gacgcatcga aggtagcgcc gcacgagtgg tatggaacgc cgacgaaagg
     1441 ttgatggcca ggatcgcatc ccacttctcg accgggaatt gctcgagtgg cgagatgtgc
     1501 tggatgccag cgttgttcac caggacatcg aggcggccga acgtatccag cgccatggcg
     1561 atcatgtcgg cgataggcgc ggcttttgac atgtccgctg gtgaatatcg gaccttaact
     1621 ccgaactcgg aaacgatttt ggcctgggtc tcggcgatat ctcctggttt gccgaagccg
     1681 ttgagcatga ccgaggagcc cgctgctgcc agcgcgcgag cgattccgag cccgattccg
     1741 ctcgtagatc ctgtgatgag ggatacgcgg ccctccagag ggcgggagct ctgttcaagg
     1801 cggagcggcg cttggatgtt catggcctga ccctttccgt tgcacgctgg cgcggcgcgc
     1861 cgtcgttatg tcgaacttgc acggtggagg gatcggcggg aaatgccggt tggctaaaga
     1921 aacgatagca agctgctttg gagcccagat agacggcggc tatggcgcat atcgccattc
     1981 ggtattttta aaacggttac aagcgcgctt ccttagcggc gctggcgcag tcacgcgcga
     2041 gtcaaactca gagggttgat ccatgctgac cagagcaaaa ttcctgcgcc tgtcgggctt
     2101 gaccgccgcg gcgctagcca tttcacc
//
LOCUS       DBNGZE010000219         4432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1697852, whole genome shotgun sequence.
ACCESSION   DBNGZE010000219 DBNGZE010000000
VERSION     DBNGZE010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1697852"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="AC16FV_05685"
     CDS             complement(<1..1497)
                     /locus_tag="AC16FV_05685"
                     /inference="COORDINATES: protein motif:HMM:NF026042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR2849584.1"
                     /translation="MSAASTIALPATTERKAKTYPVIPIVHAARASESGAQDLLPRRY
                     SLAVQLFAFAGFTLAFVGWLNETWLFLFDNPIWLNRYTEYAIILGFGLWRIYAEQNRY
                     QRRRFTVLVVMVTVFWWLIPWLRPMYEPYVGYLWGQPIFPSIHIPGTITFFLILAAVF
                     LFGRRVVCGWNCPCVGIRETVGFAFRDRTVRGQWAWRLRHSKWFFFVFYVGVMIVTLY
                     PPNSWTVSFVGLFYLIIGLTYFGTFFVAPLTGNRFYCRYLCPYGATFGLLNHAGFYDV
                     RMNREKCNDCRRCEQVCDMGIPVWEQGKKFGRITGIEDCMGCGRCVVSCPTDALEIRD
                     VRNLFRPNLVQNGSYLMKQAPAASVARVEPTTRASEVRVRDTKEIYMRPTLAEIQKQA
                     SRCLDCGVPGCRSGCPLTNRIPDWMQAAARGEFIEAAEIAHATSPFPEICGRLCPQHR
                     LCEGACTRTKEDGAVTIGAIERYVTEIALESGWTPPAPKPRSGRRVAVI"
     gene            complement(1505..2431)
                     /locus_tag="AC16FV_05690"
     CDS             complement(1505..2431)
                     /locus_tag="AC16FV_05690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2849585.1"
                     /translation="MTPSLQRFLLLPLLFLFGTPALAAPLQVQKVTDGVYALVGEFGQ
                     RSPENFGNNATFGVIVTGDGVVLVDPGGSYNGAKQIHEAIRSISDKPVQLVINTGGQD
                     HRWLGNGYFKAQGARIVASKAAVTDQRARVTDQLITLNRFVGEKLMAGTEAVHAGETF
                     ENRLDLEVGGVRLVLRHTGTAHTPGDSFVWLPQKKVVFAGDIVYVERLLVVNAESRSQ
                     SWIKAFEEMAALKPEHVIPGHGPVTTLARARADTYDYLVALRKQVKVFMDGGGEIYDV
                     GKIDQSRFKHIKLYEELKGRNAQQVFQEMEWE"
     gene            complement(2453..2689)
                     /locus_tag="AC16FV_05695"
     CDS             complement(2453..2689)
                     /locus_tag="AC16FV_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR2849586.1"
                     /translation="MTMWNDPGSHGWWWGLGALPMLLFWVLIIVAIVALGKWIFDRSG
                     DTPRGKSALDILKERYARGEIGKEEFEQKKRDLE"
     gene            complement(2686..3114)
                     /locus_tag="AC16FV_05700"
     CDS             complement(2686..3114)
                     /locus_tag="AC16FV_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR2849587.1"
                     /translation="MKKVSVMCFHDELCQVFNALMTALSLLREGSKVTLFFGSRGINA
                     VHRDKMKTLTCLPDEPKEIGEAVMTKMDEMGLPLVEDLFVMLIAEGATVLACPLNLSL
                     FGMTPKDLVDGVKVADPVKYYKEVVIPADMNLTFWPERRI"
     gene            complement(3126..4280)
                     /locus_tag="AC16FV_05705"
     CDS             complement(3126..4280)
                     /locus_tag="AC16FV_05705"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2849588.1"
                     /translation="MNSKTIVILGAGVGGVVAASLLRKSLSRAHRVVLVEREERHVFP
                     PSLLWLMTGQRTPERISRPFDALRKRGIELIRGNIEHIDPAARTVKVNGTELTGDYLV
                     IALGTEYAPDTIPGLVQAGHNFYTLGGAESLNEARLNFRAGRLVVLVSGVPFKCPAAP
                     YEAAMLLESDLRRRSVRARSTVELYSPEPGPMGVAGAEVSAMVRQMVEGKGIAYHPEH
                     VVTKVDPPARRIYFSNDTTADFDLLAYVPPHRAPKVVRDAGLCGESGWVPVNRFKLET
                     KYPGVYAIGDVTGIALAIGKPLPKAGVFAHEQARVVAHNIARAISGGNEDGIFQGHGE
                     CFVEIGDGRAGFGSGNFYAEPKPQVRLYPPSRWRHYGKVLYEQYWLRRWF"
     gene            complement(4308..4418)
                     /locus_tag="AC16FV_05710"
     CDS             complement(4308..4418)
                     /locus_tag="AC16FV_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849589.1"
                     /translation="MDARGIADTDLVTGAVRGSMAMLAEWTGWADKVIVY"
BASE COUNT          838 a         1405 c         1357 g          832 t
ORIGIN      
        1 gatcacggcg acgcgccggc cgctgcgggg cttcggcgcc ggcggcgtcc agccgctttc
       61 gagggcgatc tcggtgacgt aacgctcgat cgcgccgatc gtgacggcgc cgtcttcctt
      121 cgtgcgcgtg cacgcaccct cgcacaaacg atgctgcgga cacagccgcc cgcagatttc
      181 cgggaacggg ctggtggcgt gcgcgatttc ggcggcctcg atgaactcgc cccgggccgc
      241 ggcctgcatc cagtcgggga tgcggttggt gagcggacag ccgctgcgac agccgggcac
      301 gccgcagtcg agacaacgcg acgcctgttt ctggatctcc gccagcgtcg ggcgcatgta
      361 gatctctttt gtgtcgcgca cgcgtacttc ggacgcgcgc gttgtcggct cgacacgcgc
      421 caccgacgcg gctggagcct gcttcatgag gtaactgccg ttctgcacca gattcggacg
      481 gaacaggttg cgcacgtcgc ggatctcgag cgcgtcggtc gggcacgaca ccacgcagcg
      541 cccgcagccc atgcaatcct cgatgccggt gatgcggccg aattttttcc cctgttccca
      601 taccgggata cccatgtcgc agacctgttc gcagcgccgg cagtcgttgc atttttccct
      661 attcatgcgc acgtcgtaga agccggcgtg gttgagcagg ccgaaggtcg cgccgtaggg
      721 gcagagatag cgacagtaga agcggttgcc agtgagcggg gcgacgaaga aggttccgaa
      781 gtaggtgagc ccgatgatga gatagaacag acccacgaag ctcacggtcc aggagttcgg
      841 tggatacagc gtgacgatca tcacgccgac gtaaaagacg aagaagaacc acttgctatg
      901 gcgcagccgc cacgcccatt ggccgcgcac ggtgcggtcg cggaaggcga agccaacggt
      961 ctcgcggatg ccgacgcagg gacagttcca gccacacacc acgcgccggc cgaacaggaa
     1021 caccgccgcc aggatcagaa agaacgtgat ggtgccgggg atgtggatgg acggaaagat
     1081 cggctgaccc cagagatagc cgacataagg ctcgtacatc ggtcgcaacc agggaatgag
     1141 ccaccagaaa accgtgacca tgacaaccag caccgtgaag cgccggcgct ggtagcggtt
     1201 ctgttccgcg taaatacgcc acaggccgaa gccgaggatg atcgcgtact cggtgtagcg
     1261 gttgagccag atcggattgt cgaacagaaa cagccaggtt tcgttgagcc agccgacgaa
     1321 ggcaagtgtg aaccccgcaa aggcgaacag ttggacggcc agactgtaac gacgtggcag
     1381 caggtcctgc gcgccgcttt cgctggcgcg tgccgcatgc acgatgggaa tgaccgggta
     1441 ggtcttggct ttccgctcgg tagtggcagg cagcgcgatg gtgctggcgg cgctcatgac
     1501 cggctcactc ccactccatt tcctgaaaga cctgctgtgc gttgcggcct ttcagctctt
     1561 cgtacagctt gatgtgcttg aaacgtgact ggtctatctt cccgacgtca taaatctcgc
     1621 caccgccgtc catgaacacc ttgacctgtt tccgcagtgc gaccagatag tcgtaggtat
     1681 cggcgcgtgc ccgcgcgagc gtggtcaccg gtccgtgacc ggggatgaca tgctcgggtt
     1741 tgagcgcggc catttcctcg aacgccttga tccaggattg actgcgcgac tcggcgttca
     1801 ccacgagcag ccgctcgacg tagacgatgt cgccggcgaa aaccaccttc ttctgcggca
     1861 gccacacgaa gctgtcgccc ggggtgtgcg ccgttccggt atgacgcaac accagacgga
     1921 caccgccgac ctcgagatcg agacgatttt cgaatgtttc accggcgtgt accgcttcgg
     1981 ttcccgccat gagtttttcg ccaacaaacc ggttcagggt aatcagctgg tcggtgacgc
     2041 gggcgcgctg atccgtgacc gcggccttgg aggcgacaat tcgcgcaccc tgcgccttga
     2101 agtagccgtt gccgagccaa cggtggtcct ggccgccggt gttgatgacg agctggacgg
     2161 gcttgtcgct gatcgaccgg atcgcctcgt gaatctgttt ggcgccgtta tagctgccgc
     2221 ccgggtccac cagcacgacg ccgtcgccgg tcacgatcac gccgaaggtc gcgttgttgc
     2281 caaaattttc gggtgagcgc tgcccgaact cgccgaccag cgcgtacacc ccatcggtga
     2341 ccttctgcac ctgcaacggt gctgccagcg cgggagtgcc gaaaagaaac agcaggggaa
     2401 gcagtaaaaa ccgctggagc gaaggtgtca tttgttttcc cctcaatagg tgttattcaa
     2461 gatcacgctt tttctgttca aattcttcct tgccgatttc gccgcgggca tagcgttcct
     2521 tgaggatgtc gagtgcggac ttgccgcgcg gcgtatcgcc ggaccgatca aagatccact
     2581 tccccagcgc cacgatcgcc acgatgatca gcacccagaa cagcagcatg ggaagtgcgc
     2641 cgagacccca ccaccaaccg tgacttcccg gatcgttcca catcgtcata tcctcctttc
     2701 cggccagaaa gtcaggttca tgtcagccgg gatgaccact tccttgtagt acttcaccgg
     2761 gtcggccacc ttgacgccgt ccaccaggtc cttcggcgtc atgccgaaga gcgagaggtt
     2821 gagcggacag gcgagtacgg tcgcgccttc cgcgatcagc atgacgaaca ggtcctcgac
     2881 cagcggcaat cccatctcat ccatctttgt catcaccgct tcgccgattt ccttgggctc
     2941 atccggcagg caggtcagcg tcttcatctt gtcgcggtgc acggcgttga tgccgcgtga
     3001 gccgaagaag agcgtgacct tcgagccctc gcgcaacaga ctcagcgccg tcatcagggc
     3061 gttgaacacc tgacacagct cgtcgtggaa acacatcacg gatacctttt tcatcgggct
     3121 actccttaaa accagcgacg gagccagtac tgctcgtata gcaccttgcc gtaatgtcgc
     3181 caccggctgg gtggatagag ccggacctgc ggtttcggct cggcgtagaa atttccgctg
     3241 ccaaaacccg cgcggccatc gccgatttca acgaagcact cgccgtgtcc ctgaaatatc
     3301 ccatcctcgt ttccgccact aatggcgcgc gcgatgttgt gcgcgaccac ccgggcttgc
     3361 tcgtgggcga atacccccgc cttgggcaac ggttttccaa tggcgagcgc aatgccggtg
     3421 acgtcgccga tggcgtaaac gccgggatat ttggtttcga gcttgaaccg gttcaccggc
     3481 acccagccgc tttcgccgca caggccggcg tcgcgcacca cctttggcgc gcgatgcggc
     3541 gggacatacg ccagcaggtc gaaatccgcc gtggtgtcgt tgctgaaata aatccgccgc
     3601 gccggcggat cgactttggt aacaacatgt tcggggtggt aggcgatgcc cttgccctcg
     3661 accatctgcc gcaccatggc ggaaacctcc gcaccggcca cgcccatcgg tccgggttcg
     3721 ggcgagtaaa gctcgacggt ggagcgagcg cgcacgctac gccggcgcaa atcggattcc
     3781 agcagcatgg ccgcttcata cggtgccgcc gggcatttga agggaacgcc gctgaccaga
     3841 accaccagcc gtcctgcgcg gaaattcagg cgtgcctcgt tcagtgattc cgcgcccccg
     3901 agggtgtaga agttatgccc ggcctgaacc aggccgggga tggtatccgg cgcatattcc
     3961 gtgcccagcg cgatgacgag atagtcgccg gtcaattcgg ttccattcac cttgaccgtg
     4021 cgcgctgcgg gatcgatgtg ctcgatgttt ccccgtatca gctctatccc ccgtttccgg
     4081 agcgcatcga acggacgcga gatgcgctcc ggcgtgcgct ggccggtcat gagccagagc
     4141 agggaaggtg gaaacacgtg gcgttcctcg cgttccacca gcactacccg atgcgcgcgc
     4201 gacaggctct tccgcagcag gctcgcggcc acgacgccgc cgacgccggc gccgagaatc
     4261 acgatggtct tggagttcat tggtttcctt ttcgcgagga catgtcgtta gtacacgatc
     4321 actttatcgg cccatccggt ccactccgcc aacatggcca tgctgccgcg caccgcgccg
     4381 gtcacgagat cggtatcggc aataccgcgg gcgtcgatgc aggtgccgca gg
//
LOCUS       DBNGZE010000220        17364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_30809, whole genome shotgun sequence.
ACCESSION   DBNGZE010000220 DBNGZE010000000
VERSION     DBNGZE010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 17364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17364
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_30809"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1361
                     /gene="carA"
                     /locus_tag="AC16FV_05715"
     CDS             180..1361
                     /gene="carA"
                     /locus_tag="AC16FV_05715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR2849590.1"
                     /translation="MPSALLALEDGSLFYGESIGVPGQAVGEVVFNTAMTGYQEILTD
                     PSYARQIVTLTYPHIGNTGTNPEDMESGGVYATGLVIRDLPLALSNFRATESLPEFMR
                     RHMLIGIAGIDTRRLTRLLRERGAQNGCILAVEKGAKPDKKAALKAARAFPGLKGMDL
                     AKVVTTRKSYSWDEGLWQLGKGHVKQEHARFHVVAYDYGIKRNILRMLAARGCRLTVV
                     PAQTLADEILKMKPDGVFLSNGPGDPEPCDYAIEAIRKIVETGLPVFGICLGHQLLGL
                     ASGARTIKMKFGHHGANHPVIELGTGRVMITSQNHGFAVDETDMPSNLRVTHRSLFDG
                     TVQGLQRIDRPAFCFQGHPEASPGPHDVSPLFDRFIKMMEESDLRTKVKVIREAHKSD
                     V"
     gene            1388..4618
                     /gene="carB"
                     /locus_tag="AC16FV_05720"
                     /pseudo
     CDS             1388..4618
                     /gene="carB"
                     /locus_tag="AC16FV_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            4620..5096
                     /gene="greA"
                     /locus_tag="AC16FV_05725"
     CDS             4620..5096
                     /gene="greA"
                     /locus_tag="AC16FV_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR2849591.1"
                     /translation="MKKTPMTVAGAEKLKLELQRLKTVERPRIIQAIAEARSHGDLSE
                     NAEYHAAKEQQGFIEGRIADLDIKLATAQIIDPKTVNANGRVVFGATLNLMEEHSGQE
                     VTYQIVGDHEADIAQGKISISSPIARALIGKELGDVVEVQVPGGVRSYEILDIRYV"
     gene            complement(5196..5489)
                     /gene="yhbY"
                     /locus_tag="AC16FV_05730"
     CDS             complement(5196..5489)
                     /gene="yhbY"
                     /locus_tag="AC16FV_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR2849592.1"
                     /translation="MALNAKQRKHLKALAHHRKPVVQVGNAGVTPAVIREITQALDAH
                     ELLKIRLPGVERDARHTLLEQICEAAGAEAVQEIGRMAVIYRRAKKPRLKLPE"
     gene            5554..6177
                     /gene="rlmE"
                     /locus_tag="AC16FV_05735"
     CDS             5554..6177
                     /gene="rlmE"
                     /locus_tag="AC16FV_05735"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HIR2849593.1"
                     /translation="MARTKSSQRWLARNARDKYVKRARQEGARSRAIYKLEEIDRRDH
                     LLRPGMTVVDLGAAPGGWSQYAKSRVGDAGRVLALDILPMEPIVGVEFIEGDFMEQPV
                     LDLLMQRLQGQPVDLVISDMAPNMTGVASVDQARSMNLAELALDFSDKSLKPGGSLLI
                     KTFQGAGFNEFHAQLRRRFEKLVTRKPSASRAESKEIYLLGRGFRGA"
     gene            6293..8200
                     /gene="ftsH"
                     /locus_tag="AC16FV_05740"
     CDS             6293..8200
                     /gene="ftsH"
                     /locus_tag="AC16FV_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR2849594.1"
                     /translation="MNNLTKNLLLWLVIAIVLMSVFNNFGNRQQAARPMEYSTFIQKV
                     RQNEVDRVTIQGREVSGKLKNNESFTTYSPGDPGMIGDLIDHGVAFDAKPQEEQSLLL
                     TIFINWFPLLLLVGVWVFFMRQMQGGMGGRGAMSFGKSKARMLSEEQNKITFDDVAGV
                     EEAKEEVGELVEFLKDPSKFQKLGGRIPKGVLMTGSPGTGKTLLAKAIAGEAKVPFFS
                     ISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGHDEREQ
                     TLNQLLVEMDGFEGTEGVIVIAATNRPDVLDPALLRPGRFDRQVYVPLPDIRGREQIL
                     KVHMRKVPVADDVDANIIARGTPGFSGADLANLVNEAALFAARANKRLVDMQDFEKAK
                     DKVIMGAERRSIVMPEKERKNTAYHESGHAVVAKSLPNTDPVHKVTIIPRGRALGVTM
                     QLPTEDRYSHDRESLLSTIAVLMGGRIAEEIFMHQMTTGAANDFERATDLARNMVMRW
                     GMSDVLGTRVYGDNQSEVFLGRDVMTHKNMSNAVAEVVDKEIRRIIDEQYAKARAIIE
                     ENHDKVERMARALLEWETLESDQIEDIMTGKEPRPPAGTSGTPKGDAGSPKKPSGKTV
                     KPRLDSPAGEH"
     gene            8207..9049
                     /gene="folP"
                     /locus_tag="AC16FV_05745"
     CDS             8207..9049
                     /gene="folP"
                     /locus_tag="AC16FV_05745"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR2849595.1"
                     /translation="MTVLDCAGRPLDLRRPAVMGILNITPDSFSDGGIFLSPDKAIAQ
                     ALRMTEEGADIIDIGGESTRPGARPVSAQEEIDRVLPVVESLHKQVSLPISIDTSKPE
                     VMRAAVKAGAGFINDVRALREAGAMEAAAQLGVPVCLMHMQGEPRTMHDDPRYADIVG
                     EVRDFLKQRLDACLNTGIPAQRLVVDPGFGFGKTLEHNIELLRGLRALQDLGVPVLVG
                     LSRKSMIGKALGLPVEQRQHASVALALMAVQQGARIVRVHDVGPTVEALRMWSAVYPD
                     SSEV"
     gene            9054..10412
                     /gene="glmM"
                     /locus_tag="AC16FV_05750"
     CDS             9054..10412
                     /gene="glmM"
                     /locus_tag="AC16FV_05750"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR2849596.1"
                     /translation="MHKKRHFFGTDGVRGKVGEEPITPETVLKLGWAAGCVLGKGSAD
                     HAKILIGKDTRVSGYLLESLLEAGLSAAGVDIRLLGPMPTPAIAYLTRTARARAGIVI
                     SASHNPFDDNGIKFFSSEGTKLPDEVELAIEEKMQQPLTTAGPAALGKAKRYDDAGGR
                     YIEFCKSTFPHRLSLEGLKIVVDCANGAAYHIAPLVFSELGAEVVAVANEPDGFNINR
                     QCGATFPIFLKKTVLAHKADLGLALDGDGDRVIMVDSRGDIVDGDHLLYIIATDRKQS
                     GRLRGGVVGTLMSNLGLEHACSKLGVPFKRASVGDRYVLGLLNEHGWELGGETSGHII
                     CLDRATTGDGIIAALQVLAAMRGSGKSLTDLRSGLEIYPQTMINVRMARRFDVKESPR
                     VQQAIKEVESELADNGRVVLRASGTEPVVRVMVEGRDAAQVERLTRHLADTVKSAAEE
                     AA"
     gene            10664..11422
                     /gene="tpiA"
                     /locus_tag="AC16FV_05755"
     CDS             10664..11422
                     /gene="tpiA"
                     /locus_tag="AC16FV_05755"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR2849597.1"
                     /translation="MVQRRPLVAGNWKMNGSRTESAALLDGVRKGISHASKAEVAVCP
                     PFILIALAAEKLAGSPVVWGGQNLSVHKSGAYTGEISGPMLRDYGCTYVIVGHSERRT
                     LYGENDAVVSEKYGAAQTAGLIPILCVGETLQERETNQTEAVVARQLDAVLGKHGIGS
                     FRNAVTAYEPVWAIGTGKTATPQQAQDVHRFIRSRLMARDKAVAEGTRILYGGSMKGS
                     NAKELLAQPDIDGGLIGGASLQVEEFLTICRAAD"
     gene            11478..11825
                     /gene="secG"
                     /locus_tag="AC16FV_05760"
     CDS             11478..11825
                     /gene="secG"
                     /locus_tag="AC16FV_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR2849598.1"
                     /translation="MRDFVLVIDVISAVVLIGLILLQQGKGADIGAAFGSGASQTLFG
                     SRGSANFLTRTTAILGTVFFLSNLGLAWLYAQQTVPTSVTKSVVIEQPAKTEVPVPPA
                     DVPAAPKAPEVPK"
     gene            11867..11951
                     /locus_tag="AC16FV_05765"
     tRNA            11867..11951
                     /locus_tag="AC16FV_05765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11901..11903,aa:Leu,seq:gag)
     gene            12178..12534
                     /locus_tag="AC16FV_05770"
     CDS             12178..12534
                     /locus_tag="AC16FV_05770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR2849599.1"
                     /translation="MLQAYLPILIFIGVALVMGGVVILAGRVLGPHRPDSAKLSPYEC
                     GFEAFEDTRMKFDVRYYLVAILFIIFDLEIAFLFPWAVALKEIGLFGFLSMMVFLGIL
                     VVGFIYEWKKGALEWE"
     gene            12525..13001
                     /locus_tag="AC16FV_05775"
     CDS             12525..13001
                     /locus_tag="AC16FV_05775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR2849600.1"
                     /translation="MGIEGHFEKGWVTTQADKLINAARTGSLWPMTFGLACCAVEMMH
                     AAAARYDMDRFGIIFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRTNTIAR"
     gene            13022..13627
                     /locus_tag="AC16FV_05780"
     CDS             13022..13627
                     /locus_tag="AC16FV_05780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR2849601.1"
                     /translation="MTDSNQDLAAHAQELFGAAITDVRESLGELTLEVRRDEIIPVCT
                     RLRDDPRLAFAQLMDLCGVDYLDYGRESPAGPRKGPRFAVVYHLLSLKHNRRIRLRVY
                     LDDEVPLIASVVGIWNSAAWYEREAFDLFGIVFEGHPDLRRILTDYGFVGYPFRKDFP
                     LIGQVEMRYDPEKQRVVYQPVSIEPRVLVPRVIREEGFAKS"
     gene            13771..15024
                     /locus_tag="AC16FV_05785"
     CDS             13771..15024
                     /locus_tag="AC16FV_05785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR2849602.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVVVRADPHIGLLH
                     RATEKLAESKPYNQSIGYMDRLDYVSMMSNEHAYVLAIEKLLGIEPPLRAQYIRVMFD
                     EITRLLNHIFWIGAHALDIGAMTVFLYAFREREDLFDIYEAATGARMHATYYRPGGGY
                     RDLPDSMPQYQPSKFRSAADLTRLNSNRQGSLLDFIWDFTERFPANVDEYETLLTDNR
                     IWKQRTVGIGVVSPERALQLGFTGPMLRGSGVEWDLRKKQPYEVYDRLDFDIPVGVNG
                     DCYDRYLVRVEEMRQSNRIIRQCVEWLRRNPGPVMIDDHKIAPPRREEMKADMEALIH
                     HFKLFTEGYCIPAGEAYATIEHPKGEFGVYLISDGANKPYRVKIRSAGFAHLAAMEEM
                     VRGHMIADVVAIIGTMDIVFGEIDR"
     gene            15024..15530
                     /gene="nuoE"
                     /locus_tag="AC16FV_05790"
     CDS             15024..15530
                     /gene="nuoE"
                     /locus_tag="AC16FV_05790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR2849603.1"
                     /translation="MPQSRPASKIHLLSDATRAEIDRWIAKYPPDRKQAAVMAALRIA
                     QDQNGGWLPVDLIEAVAEYLEMPPIAVHEVATFYTMYDLKPVGRHKVCVCTNISCQLN
                     GSARIMDHLQKKLGVKPGETTSGGKFTLKEVECLGACGGAPMMMVDKTYYENLTPEKV
                     DEILDRLE"
     gene            15535..16821
                     /gene="nuoF"
                     /locus_tag="AC16FV_05795"
     CDS             15535..16821
                     /gene="nuoF"
                     /locus_tag="AC16FV_05795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR2849604.1"
                     /translation="MANELCFRNIHLDQPWRLSVYQGSGGYETWKKILQEKTPPEKII
                     DELKKSALRGRGGAGFSTGLKWSFMPRNTPGQKYIVCNSDEGEPGTFKDRDILRFNPH
                     ALIEGMAIAGYAIGATVGYNYIRGEFWEPYERFEAALKEAYAAGLLGKNVLGSGVDFD
                     LYTHLGAGAYICGEETALLESIEGKKGQPRFKPPFPANYGLFGRPTTINNTETLASVP
                     DIIRNGADWFLNLGKPNNGGPKLFSVSGHVNKPGNYEVPLGTPFAKLLEIAGGVRHGH
                     HLKAVIPGGSSANVLPAGIMMEVTMDYDSLAKAGSMLGSGGVIVMDDSTCMVHALARI
                     AHFYYEESCGQCTPCREGTGWMSRVIHRIEHGQGRPEDLDMLTDVASNIEGRTICALG
                     DAAAWPVKSFIKYFRSEFEYHIEHRRCMPGTHGSHA"
     gene            16818..>17364
                     /locus_tag="AC16FV_05800"
     CDS             16818..>17364
                     /locus_tag="AC16FV_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR2849605.1"
                     /translation="MSANPKDVKTPPVSDTVTIEVDGRKLSARKGQMLIEVTDASGIY
                     IPRFCYHNKLSVAANCRMCLVDVEKAPKPLPACATPVMDGMVVHTRSEKARTAQKGTM
                     EFLLINHPLDCPICDQGGECDLQDQAMAYGVDASRFHERLSIAGNCRMCLVELKGSPK
                     PIASCAWGVRDCRPGPKGEPPE"
BASE COUNT         3526 a         5072 c         5425 g         3341 t
ORIGIN      
        1 aatgacgcgc cgcgcgcggc gcttccgtgt ttcgtcgttg attccagccg ggctgtcacg
       61 taaaattccg cttcagccgg aaacaccctt attttaggta ttcaaggcgg gagagaagca
      121 ccaacagcac ggcgcctctc ccgtttttgt gtgcctgcgc gtcgagaggt aattggtata
      181 tgccgtctgc actgcttgcg ctggaagatg gttctctgtt ctacggggag tccatcggcg
      241 ttccggggca ggcggtgggc gaggtggtgt tcaacaccgc catgaccggc taccaggaaa
      301 tcctcaccga tccttcctac gcgcgccaga tcgtcacgct gacttatccc cacatcggca
      361 ataccggcac caaccccgag gacatggaat ccggcggcgt ttatgccacc ggccttgtca
      421 tccgtgacct gccgctggcc ctcagcaatt tccgcgcgac ggaatcgctg ccggaattca
      481 tgcgccgtca catgctgatc ggcatcgccg gtatcgacac gcggcgactg acgcgcctgt
      541 tgcgcgaaag gggcgcgcag aatggatgca tcctggccgt cgaaaagggc gcaaagcccg
      601 acaagaaagc ggcactgaag gccgcgcgcg ctttcccggg cctcaagggc atggaccttg
      661 ccaaggtagt gacgacgcgc aagtcctaca gctgggacga aggcctgtgg cagctgggca
      721 agggccatgt gaagcaggag catgcacggt tccatgtggt tgcctacgac tatggcatca
      781 agcgcaacat cctgcgcatg ctggccgcgc gcggctgccg gcttacggtg gtgccggcgc
      841 agaccctggc ggacgagata ttaaagatga aaccggacgg tgtgtttctc tcgaatggtc
      901 ctggcgatcc ggagccgtgc gactacgcca tcgaggcgat tcggaaaatc gtggaaacag
      961 gtttgccggt gtttggcatc tgcctgggtc accagctcct gggcctggcc tccggtgcgc
     1021 gtactatcaa aatgaaattt ggccaccatg gcgcgaacca tccggtgatc gaactcggga
     1081 caggtcgcgt catgattacc agccagaacc atggttttgc ggtggacgaa acggacatgc
     1141 cgtccaatct tcgcgtgacg caccgctcgc tgttcgatgg cacggtgcag gggttgcagc
     1201 gtatcgacag gccggcgttc tgtttccagg gccatcccga ggccagcccc ggcccgcacg
     1261 acgtgtcgcc gctgttcgac cggtttatca agatgatgga ggaatcagac cttagaacca
     1321 aggtcaaggt catcagggag gcccacaaat ctgatgtttg aactatggac tcggatttga
     1381 cgcaacaatg ccaaaacgta ctgacataaa aagcatactg atcgtgggcg cggggccgat
     1441 catcatcggc caaggctgcg agttcgacta ttcgggcgcg caggcctgca aggcgctcaa
     1501 ggaagagggc taccgtgtca tcctcgtcaa ttccaatccg gccacgatca tgaccgatcc
     1561 ggagatggcc gacgcgacct atatcgagcc cattacctgg ccgacgattt ccaggatcat
     1621 cgagaaggag cgccccgacg cgctgctccc gaccatgggc ggccagacgg cgcttaatac
     1681 cgcgctcgac ctggcgcgtg aaggcgtgct ggaaaaattc ggtgtcgaaa tgatcggcgc
     1741 caagcgtgaa gcgatcgaca aagccgagga ccgcgaacgg ttcaagaagg ccatggagtc
     1801 catcggcctg ccatgcgcgc gcggcgcgat cgcccacagc atggaagaag ccatgcaggt
     1861 gcaggcggcg atcggtttcc ccgtcatcat ccgcccttcg ttcaccctgg gcggcaccgg
     1921 cggcggcatc gcctacaaca aggacgaatt cacggtcatc tgcgagcgcg ggctggatgc
     1981 ttcgcccacg cgcgaattgc tcatcgagga atccatcatc ggctggaagg aattcgagat
     2041 ggaggtggtg cgcgaccaca aggacaactg catcatcatc tgttcgatcg agaatctcga
     2101 tcccatgggc gtacatacgg gcgattcgat cacggtcgcg ccggcgcaga cgctcaccga
     2161 caaggaatac cagctgatgc gcgatgcctc gatcgcctgc ctgcgcgaga tcggggtgga
     2221 caccggcggc tcgaacgtgc agttcgcgat caatccgaag gacgggcgca tgatcatcgt
     2281 ggagatgaat ccgcgtgtgt cgcgttcttc ggcgctggcc tccaaggcca caggttttcc
     2341 gattgcgaaa atcgccgcca aactcgcggt cggttacacg ctggatgagc tgaaaaacga
     2401 catcaccggt gggaagacgc cggcctcgtt cgagcccacc atcgattacg tggtaaccaa
     2461 gattccgcgc tttaccttcg agaaattccc caaagccgat ccgacgctca ccacccagat
     2521 gaaatcggtg ggcgaggcga tggcgatcgg ccgcaccttt caagaatcgt tccagaaggc
     2581 gctgcgctcg ctcgaaaccg gaagctacgg tctcgaatcg cggcttgatc ccgccgcgga
     2641 caaggacgaa acgcgcaagc gcctgtcgct ggccctgcgt gtaccgggtt cggagcgcgt
     2701 ctggcatgtc gccgatgctt tccggttcgg catgggtcag caggagattc atgacctttg
     2761 ttacatcgat ccctggttcc tggcgcaaat cgccgatctg gtggagaccg agaaaacgat
     2821 cgcgaagtat ggcaggaaaa agccgtccgc cgatctgctg cgcgcctgga agcgcaaggg
     2881 attttccgac cgccgcctgg cgcaattgat gaatgtgaag gaaaaacagg tgcgccaatg
     2941 gcgccatgcc gccaaggtgc gcccggtcta caagcgcgtc gattcctgtg ccgccgaatt
     3001 tgcctcggcc acggcctata tgtattccac ctatgaagag gagtgcgagg ccgcgcccga
     3061 gaaaaggaaa aagatcatcg tgctgggtgg cggcccaaac cgcatcggcc agggcatcga
     3121 gttcgattac tgctgcgtgc acgcctcgtt tgcgctgcgc gaggatggtt accagaccat
     3181 catgatcaac tgcaacccgg aaacggtgtc aaccgactac gacacctccg accggctgta
     3241 cttcgagccg ctgacgctgg aagacgtgct tgaaatcatt cgcctggaga aacccgtggg
     3301 cgtgatcgtg cagtacggcg gccagacgcc gctcaagctc gcgcgcgggc ttgaggcggc
     3361 gggcgcgccg atcatcggta cgacgccgga atccatcgac gtggccgaag atcgcgaacg
     3421 gttccagaag ctgatcaaaa aactgaaact gctgcagcca ccgaacgcca cggcgcgcac
     3481 cgcgcaggag gcggtgaaac tggccgacaa gatcggctat ccgctggtgg tgcgcccttc
     3541 ctatgtgctg ggtgggcgcg ccatggaaat cgtgcacgcg cgcgaggacc tggagaccta
     3601 gatgcgcgtg gcggtgcgcg tgtccgagga ttcgccggtg ctgctggacc gcttcctcaa
     3661 cgatgcgacc gaagtggacg tcgacgccat ctccgacggc aagcaggtca tcatcggcgg
     3721 catcatggaa catatcgagg aggccggcgt gcattctggc gattcggctt gctcgttgcc
     3781 gccgttttcc ctgtcgaaga agatccagga tgagctgcgc aaccagaccg tcaagatggc
     3841 caaggcactc aaggtggtcg gcctgatgaa cgtgcagttc gccatccaga atggcaagat
     3901 ttacgtgctg gaggtgaacc cgcgcgcctc gcgcacggta ccgtacgtgt ccaaggccac
     3961 cgggcggccg ctggccaaga ttgcggcgcg ctgcatggcg gggcagtcgc tcaagaagca
     4021 gggtgtgacg ggagagattg tgcctgagta ttattcggtg aaggaggcgg tgttcccctt
     4081 catcaagttc cccggcgtgg acacgattct gggcccggag atgaaatcga ccggcgaggt
     4141 catggggatt ggccgcagtt tcggcgaggc ctttaccaaa tcacagatcg ccgcgggcag
     4201 tccgatcccg aacagcggcc tcattttcat cagcgtgcgc gacgcggatc agaaaggggt
     4261 ggtcgaggtc gcgcgctact tcagcaagca cggattcgag attctggcca cccgtggaac
     4321 cgccgcggtg ctcgaggccg gcggcgtggc gacgcgcgcg gtcagcaagc tgtccgaggg
     4381 gcggccgaac atcatcgaca tgatcacgaa tgacgagatt gacatcatca tcaatacggt
     4441 ttctgataaa cagagcctcg ccgattcata cgctatccgg cgcgaggcgt tgcagcacaa
     4501 ggtgaccaac tacacgacgc tggcggcggc caaggctgcc tgtgaggcgc atcgtgccgc
     4561 cggacgcatg cgcgtcagcc ggttgcagga cctgcataag gaattggtag gaaattagta
     4621 tgaagaaaac gcccatgacc gttgccggcg ccgagaagct caagcttgag ctgcagcgcc
     4681 tgaagaccgt cgagcgcccg cgcatcatcc aggcgatcgc ggaagcgcgt tcgcacggcg
     4741 acctatcgga gaacgccgaa tatcatgcgg ccaaggagca gcagggcttt atcgagggac
     4801 gcatcgccga tctcgatatc aagctggcga cggcgcagat catcgatccc aaaaccgtca
     4861 atgccaacgg ccgtgtggtt tttggcgcca cgctgaatct catggaagag cattccggcc
     4921 aggaagtcac ctaccagatc gtcggcgacc acgaggctga catcgcgcag gggaagatct
     4981 ccatcagttc gcccatcgcc cgcgcactga tcggcaagga gctgggtgac gtggtcgagg
     5041 tccaggtgcc gggtggcgtg cgctcttacg aaatcctaga cattcgatac gtttgaattc
     5101 acaggattaa aacaggatta acaagattaa aaccaaaaac tttacaattt aatcctgtaa
     5161 atcctgaaaa atcctgttaa tcctgtctat ttattttatt ccggaagctt caggcgcggt
     5221 tttttcgcgc ggcggtagat gaccgccatc cggccgattt cctgcacggc ctcggccccg
     5281 gcggcttcgc agatttgctc cagcaatgtg tgtcgtgcat cgcgttccac gccaggaagc
     5341 cgtatcttga gcagctcgtg ggcatcgagg gcctgtgtta tttccctgat taccgcgggg
     5401 gtgacaccgg catttcccac ctgcaccacg ggtttgcggt gatgggccag cgccttcagg
     5461 tgtttccgct gttttgcatt caaagccata tatataatga tcatcacttc ccgataatcc
     5521 cgcgcatcat accgcagcct cgcccgatcc cccatggccc gcaccaagag cagccagcgc
     5581 tggctggcac gcaacgccag ggacaaatac gtcaaacgcg cgcgtcagga aggcgcccgc
     5641 tcgcgtgcga tctacaagct tgaggaaatc gatcgccgcg atcatctgct gcgcccgggc
     5701 atgacagtgg tcgatctggg cgccgcgcct ggaggctggt cgcagtacgc caaaagccgc
     5761 gtcggcgatg ccggacgggt gctggcgctg gacatcctgc cgatggaacc gattgtcggc
     5821 gtcgaattca tcgagggcga tttcatggaa cagccggtgc tagacttgtt aatgcagcgt
     5881 ctccagggtc aaccggtgga ccttgtaatc tccgacatgg cccccaatat gacgggtgtg
     5941 gcctccgtgg atcaggcgcg cagtatgaat ttggcggaac tcgccctcga tttctccgac
     6001 aaatcactga agcccggagg gtcgttgctg atcaagacct ttcagggcgc ggggtttaac
     6061 gagtttcacg cgcaattgcg tcgccgcttc gagaagctcg tcacgcgcaa accgtcggcg
     6121 tcacgcgcgg agagcaagga aatttatctt ctgggcaggg ggtttagggg cgcctgagca
     6181 aaaacgttgt ttgccgggag acatcatagg aaaatgcgga tattgaatga ctggctgata
     6241 aaaatgtagc cctacttcgt tcgtcctgac atattatatt aagaggtaat acttgaacaa
     6301 tctcaccaag aatctcctgt tatggctcgt catcgccatc gtgctgatgt cggtgttcaa
     6361 caatttcggc aaccgccagc aggccgcgcg cccgatggaa tactccacct tcatccagaa
     6421 ggtgaggcag aatgaggttg atcgcgtcac catccaggga cgcgaggtca gcggcaagct
     6481 gaagaacaac gaatcgttca cgacctactc tcccggcgac cccggcatga tcggcgacct
     6541 gatcgaccat ggcgtggcgt tcgatgccaa gccgcaggaa gagcaatcgc tgttgctgac
     6601 cattttcatc aactggttcc cgctgctgct gctggtgggc gtgtgggtgt tcttcatgcg
     6661 ccagatgcag ggcggcatgg gcggccgtgg cgccatgagt ttcggcaaga gcaaggcgcg
     6721 catgctttcc gaggaacaga acaagatcac cttcgacgac gtggccggcg tggaagaggc
     6781 caaggaagag gtgggcgaac tggtggaatt cctcaaggac cccagcaagt tccagaaact
     6841 cggcggccgc atccccaagg gcgtgctcat gaccggatcg cccggcaccg gcaagacgct
     6901 gctcgccaag gccatcgccg gcgaggccaa ggtgccgttc ttctcgattt ccggttccga
     6961 cttcgtcgag atgttcgtcg gcgtgggcgc ctcgcgcgtg cgcgacatgt tcgagcaggc
     7021 gaagaagcac gcgccctgca tcatcttcat cgatgaaatt gacgctgtcg gtcgccagcg
     7081 tggcgccggc ttaggcggcg gccatgacga gcgtgagcag acgctgaacc agttgctggt
     7141 ggaaatggac ggtttcgagg gcaccgaagg tgtgatcgtt atcgccgcga ccaaccgccc
     7201 ggacgtgctc gacccggcct tgctgcgccc gggccgtttc gaccgccagg tgtacgtgcc
     7261 gctgcccgac atccgcgggc gcgaacagat cctcaaggtg cacatgcgca aggtgccggt
     7321 ggccgacgac gtggacgcca atattattgc ccgtggcacg cccggtttct ccggtgcgga
     7381 tcttgcgaac ctcgtgaacg aagcggcgtt gttcgccgcg cgcgccaaca agcgcctcgt
     7441 ggatatgcag gatttcgaga aggccaagga caaggtcatc atgggcgcgg agcgtcgctc
     7501 catcgtcatg ccggaaaagg aacgcaagaa cacggcatac catgaatccg gtcacgcggt
     7561 agtggcgaag tcgctgccga acaccgatcc ggtacacaag gtgaccatta tcccgcgcgg
     7621 tcgcgcgctg ggcgtcacca tgcaactgcc gaccgaggac cgctacagtc acgaccgtga
     7681 aagcctgctc tcaaccatcg cggtgctgat gggtggccgt attgccgagg aaatattcat
     7741 gcaccagatg accaccggag cggccaacga ttttgagcgc gccaccgacc tcgcgcgcaa
     7801 catggtcatg cgctggggca tgtccgacgt gctcggcacg cgtgtctacg gtgacaacca
     7861 gagcgaggta ttccttgggc gtgacgtgat gacgcacaag aacatgagca atgccgtggc
     7921 cgaggtggtg gacaaggaaa ttcgccgcat catcgatgag caatacgcca aggcgcgcgc
     7981 catcatcgag gagaaccacg acaaggtcga gagaatggcg agagccttgc tcgagtggga
     8041 gacgctcgaa tccgaccaga ttgaggacat catgacgggc aaggagccgc gtccgccggc
     8101 aggtacgagc ggtacgccca agggtgatgc cggcagtccg aagaaaccga gcggcaagac
     8161 cgtgaagccg cgtctcgact cacccgcggg tgaacactga gctgaaatga ctgtcttgga
     8221 ttgcgccggg cggccgctcg atctgcggcg tcccgcggtc atgggcatcc tgaacatcac
     8281 ccccgactcc ttttcggacg ggggtatttt tttgtctccc gataaagcga ttgcccaggc
     8341 gttgcggatg acggaggagg gcgccgatat tatcgatatc ggcggcgaat ccactcgtcc
     8401 cggcgcccgg ccggtatcgg cgcaggagga aatcgatcgg gtgcttcccg tggtggagtc
     8461 gcttcacaag caagtctccc tccctatttc gatagacact tccaagccag aagtcatgcg
     8521 cgccgccgtc aaggcgggcg cgggatttat caacgatgtg cgtgcgctgc gtgaagccgg
     8581 agcaatggaa gcggcggcgc agctcggcgt gccggtatgc ctgatgcaca tgcagggcga
     8641 gccgcgcacc atgcatgacg atccacgcta cgccgatata gtcggcgagg tgcgggattt
     8701 cctgaaacaa cgcctggacg cctgtctgaa cacgggtatc ccggcgcagc ggcttgtggt
     8761 cgatcccggc ttcggtttcg gcaagacgct ggaacataat atagaattac tgcgtggcct
     8821 gagggcgctg caagacttgg gcgtgccggt gctggtcggg ttgtcgcgta aatccatgat
     8881 cggcaaggcg ctgggtctgc cggtggagca gcgacagcac gcaagcgtgg cgctcgccct
     8941 gatggcggtt cagcaaggtg cccgcatcgt gcgcgtgcac gacgtcggtc cgaccgtcga
     9001 ggcactgcgc atgtggtcag cggtgtatcc ggacagtagt gaggtgtaaa gatatgcaca
     9061 agaagcgtca ttttttcggc accgatggtg tccgcggcaa ggtgggtgaa gagccgatca
     9121 cgccggaaac cgtgctcaag ctcggttggg ccgcaggctg cgtgctcggc aagggttcag
     9181 ccgatcacgc caagattctc atcggcaagg acacgcgtgt ttccggttat ctgctggaat
     9241 ccctgctgga agccgggctc tcggccgccg gcgtggacat ccggctgctc ggaccgatgc
     9301 cgacgcccgc gattgcctac ctcacgcgca ccgcccgcgc ccgcgccggc atcgtgattt
     9361 ccgcctcgca caatcccttc gacgacaacg gcatcaagtt tttttcctcc gagggcacca
     9421 agttgccgga tgaggtcgag ctggcgatcg aggaaaagat gcaacagccg ttgacgaccg
     9481 ccggccccgc ggcgttgggt aaggccaagc gctacgatga tgccggcggt cgctacatcg
     9541 agttttgcaa gagcaccttc ccccaccgtc tgagccttga agggctcaag atcgtggtgg
     9601 attgcgccaa cggcgcggcc taccacatcg cgccgctggt attcagcgag ctcggcgccg
     9661 aggtggtggc ggtcgctaac gagccggacg gtttcaatat caaccgccag tgcggcgcca
     9721 ccttcccgat ttttctcaag aagacggtgc tggcgcacaa ggccgatctc gggttggcgc
     9781 tcgacggcga cggcgaccgg gtgatcatgg tcgacagccg gggtgacatc gtggatggtg
     9841 accacctgct ctatattatc gccacggacc ggaagcagtc cggtcggctg cgcggcggag
     9901 tggtgggcac gctcatgagc aatctcggac tggagcacgc ctgcagcaag ctgggcgttc
     9961 ctttcaagcg cgcctcggtc ggcgaccgtt atgtcttggg cctgctcaac gagcatggct
    10021 gggaactggg cggcgagact tccggccata tcatctgtct cgatcgcgcc accacgggcg
    10081 acggcatcat tgccgcgctg caagtgttgg cggccatgcg cggctccggt aaaagcctca
    10141 ctgatctcag gagcggtctc gagatctatc cgcagaccat gatcaacgtg cgcatggcgc
    10201 ggcgtttcga cgtcaaagaa tcgccgcgcg tgcagcaggc catcaaggaa gtcgaaagcg
    10261 agctggcgga caacgggcgc gtggtgctgc gtgcctccgg taccgagccc gtggtgcgcg
    10321 tcatggtcga gggccgcgac gccgcgcagg tcgagcgcct cacacggcat ctcgccgata
    10381 ccgtcaagag cgccgccgaa gaagcggcct gaggaatacg catcttaatg acaggacgaa
    10441 cggagcgaag cggagtaggg ctactgctat ccgcggatgc acgctgatat ttaataagat
    10501 gaacgcagat ttgaagagat aaacgagaca tttcttgttt atctgcgttt gtctgcgacg
    10561 tcttcagcgt tcatctgcgg tcccataatt gctttcccgc cccggcatcg gtaacatcgc
    10621 gcccgttttt cgagagattt aggacgaata cggggaggtt ccgatggtgc agcgccggcc
    10681 gctggtggcg ggtaactgga aaatgaatgg ttcgcggacg gagtccgcgg ctctgctgga
    10741 tggagtcagg aaaggcatca gccatgcgtc caaggcagaa gtggcggtgt gtcccccgtt
    10801 tatcctgatt gcgctggcgg cggaaaaact ggccggcagt ccggtggttt ggggtggtca
    10861 gaatctttcc gtgcacaaat ccggggccta taccggcgaa atctccggtc cgatgctcag
    10921 ggattatggc tgcacatatg ttatcgtcgg acattccgag cggcgcacgc tatatggcga
    10981 gaacgacgcc gtggtgtctg agaagtacgg cgcggcccag accgcgggcc tgattcctat
    11041 attatgtgtc ggcgagaccc tgcaggaacg cgagacgaat cagaccgagg cagtggtcgc
    11101 gcgacagctc gacgctgttc ttggcaagca tggcatcggc agtttcagga atgccgtgac
    11161 cgcctatgaa cccgtatggg ccattggcac cggcaaaaca gcgacgcccc agcaggcgca
    11221 ggatgtgcat cgttttatcc gcagccggtt gatggccagg gataaagccg ttgccgaagg
    11281 gacgcgcatt ctttatggcg gcagcatgaa gggcagcaat gcgaaggaac ttctggccca
    11341 gcctgatatc gacggaggtc tgatcggcgg ggcgtcgttg caggtggaag agttcctgac
    11401 gatttgccgc gctgccgact gaaagagata tattttatga caggacgaac ggagcgaagc
    11461 ggagtagggc tacatttatg agagattttg tactggtgat tgatgttatt tcggccgtgg
    11521 tgctgatcgg cctgattctg ttgcagcagg gcaagggcgc ggatatcggc gcggccttcg
    11581 gcagcggtgc ctcgcagacc ctgttcggca gccgcggctc ggccaatttt ctgacgcgta
    11641 ccaccgcgat tctgggaacg gttttctttc tctccaacct cgggctggcc tggttgtatg
    11701 cgcagcagac ggtgccaacg agcgtcacga agagtgtggt cattgagcag ccggcgaaga
    11761 cagaggtacc ggtgccgccc gctgatgttc ccgccgcgcc caaggcgccc gaggtgccca
    11821 agtagttccg gcaacatgtt cccggtggct ggagatcttt agcagagccg acgtggtgaa
    11881 attggtatac acgctacctt gaggtggtag tggcgaaagc cgtgagagtt cgagtctctc
    11941 cgtcggcacc atttaattca tgtttgatcc cgcgcgcatt gtgtgacgta aaaaatacgc
    12001 acatactgtt tgcatcaata ggcgttcttt atacccccaa aagcgcctta accctttgaa
    12061 gggtatcggt ttgtaatcct gcggtttgct tgacatcctt tgcagcgaaa gcctagactc
    12121 gctcgctcat tcaatcatga gcggatcaaa atccgccgac gaattcctgg ggcccggatg
    12181 ctgcaagctt atctccccat cctgattttt atcggcgtcg ctctggtcat gggcggtgtt
    12241 gtcattctgg ccggtcgggt gctcggaccg caccgcccgg acagcgccaa gctttctccc
    12301 tacgagtgcg gtttcgaggc gttcgaggac acgcgcatga agttcgacgt gcgttattac
    12361 ctcgtcgcca tcctgttcat cattttcgat cttgaaatcg cgttcctgtt tccttgggcc
    12421 gtggcgctca aggaaatcgg cctgttcggg ttcctttcca tgatggtgtt tctcggcatc
    12481 ctcgtcgtcg gcttcatcta cgagtggaag aagggggctc tggagtggga atagagggtc
    12541 actttgaaaa aggctgggtc acgacgcagg cggacaagct gatcaatgcc gcgcgtacgg
    12601 gttcactctg gcccatgact ttcggactcg cctgctgcgc ggtcgagatg atgcacgccg
    12661 ctgccgcgcg ctacgacatg gaccgctttg gaatcatatt ccggccaagc ccgcgccaat
    12721 cggatgtcat gatcgtggcc ggtaccctgg tgaacaagat ggcgccggcc ttgcgcaagg
    12781 tctatgacca gatggccgag ccgcgctggg tgatctccat gggttcctgc gccaatggcg
    12841 gcggctatta ccattattcc tattccgtgg tgcgtggctg cgatcgcatc gtgccggtgg
    12901 atgtctacgt tcccggctgc ccgccgacgg ccgaggcctt gctgtatggc atcatccagc
    12961 tgcaaaacaa gattcgtcgc accaacacca tcgcccgcta gcgcccgagg gaatccggac
    13021 catgactgat tcgaatcagg accttgccgc tcacgcccag gaattattcg gcgcggcaat
    13081 cacggatgtg cgtgaatcgc tcggagagct gacgctggaa gtgcggcgtg acgaaattat
    13141 tccagtctgc acccgcttgc gtgacgaccc tcggctcgcc ttcgcccagt tgatggatct
    13201 gtgtggcgtg gattatctcg attacgggcg tgaatcgccg gcgggcccgc ggaaaggacc
    13261 gcggttcgcc gtggtgtatc acctgctgtc actgaagcac aaccgacgga tccgcttgcg
    13321 tgtgtacctc gatgacgaag tgccattgat tgcctcggtg gtaggcattt ggaattccgc
    13381 cgcctggtac gaacgcgagg ccttcgatct cttcggcatc gtgttcgagg ggcacccgga
    13441 cctgcgccgc attctcaccg attacggttt cgtggggtat cccttccgca aggattttcc
    13501 gctcatcggc caggtcgaga tgcgctacga ccccgagaaa cagcgcgtcg tgtatcaacc
    13561 ggtgtccatc gagccgcgcg tactggttcc acgcgtgatt cgtgaagaag gttttgccaa
    13621 aagctgatcc cgtggccgag attagaaatt acacgatgaa ttttagtgtc ggccgcgctt
    13681 cgcgcttagc attcgctgcg cggatgttag cccccattga agttcatcgt ggacataaca
    13741 taactcacgt tgcacaagag gttgaacatc gtggctgaga ttcggaacta cacgatgaac
    13801 tttggccccc agcaccccgc ggcccacggg gtgttgcgcc tggtgctgga actggatggc
    13861 gaggtggtgg tgcgcgccga tccgcatatc ggcctgctgc atcgcgccac cgagaagctg
    13921 gctgagagca agccgtacaa ccagtccatc ggctacatgg accgtctcga ctacgtgtcc
    13981 atgatgagca acgagcacgc ctatgtgctc gccatcgaga aactgctcgg catcgagccg
    14041 ccgctgcgcg cgcagtacat ccgggtgatg ttcgacgaga tcacgcgcct gctcaatcat
    14101 atattctgga tcggcgcgca cgcgctcgat atcggcgcga tgacggtgtt cctgtacgcg
    14161 ttccgcgagc gcgaggatct gtttgacatc tacgaagcgg cgaccggtgc tcgcatgcac
    14221 gccacctatt accgcccggg cggcgggtat cgagacctgc ctgattccat gccgcaatac
    14281 caaccgtcga aattccgcag tgccgcggat ctgacccgac tcaacagcaa tcgccagggt
    14341 tcgctgctcg atttcatctg ggacttcacg gaacgcttcc cggccaacgt cgatgagtac
    14401 gagacgctgc tgactgacaa ccgtatctgg aagcaacgca ccgtgggcat cggcgtggtg
    14461 tcgccggaac gcgccctgca actcggcttt accgggccga tgctgcgcgg atcgggtgtc
    14521 gaatgggact tgcgcaagaa acagccctac gaggtttatg accgtctgga tttcgacatt
    14581 cccgtgggcg tcaacggcga ctgctacgat cgctatctcg tgcgcgtgga ggagatgcgt
    14641 caatcgaacc ggatcatccg ccagtgcgtg gaatggctgc gccggaatcc ggggccggtg
    14701 atgatcgacg atcacaagat tgctccgcca cggcgcgagg aaatgaaagc cgacatggaa
    14761 gcgttgatcc atcatttcaa actgttcacc gagggttact gcattccggc gggcgaggcc
    14821 tacgccacca ttgagcaccc gaagggcgag ttcggcgtgt acctgatatc agacggcgcg
    14881 aacaaacctt accgcgtgaa aatccgctcc gccggtttcg cgcacctcgc ggcgatggaa
    14941 gagatggtgc gcggacacat gatcgccgat gtggtggcga tcatcggcac catggatatc
    15001 gtgtttgggg agatcgatcg gtgatgccgc aatccagacc ggcctcgaag atccatttgc
    15061 tctcggatgc cacgcgcgcc gagatcgacc gctggatcgc caagtatccg cccgaccgga
    15121 agcaggcggc cgtcatggcg gcgctgcgca tcgcccagga ccagaacggc ggctggctcc
    15181 cggtggattt gatcgaggcg gtggcggaat atctcgagat gccgccaatc gcggtgcatg
    15241 aagtggcgac gttctacacc atgtatgacc tcaagcccgt ggggcgccac aaggtttgcg
    15301 tctgcaccaa tatttcctgc caactgaacg gttccgcccg catcatggat cacctgcaga
    15361 aaaagctcgg ggtgaaaccc ggggaaacca cgtcgggcgg caagttcacc ctgaaggaag
    15421 tcgaatgcct gggcgcctgc ggcggcgcgc ccatgatgat ggtcgacaag acttattatg
    15481 agaatctcac accggagaag gtggacgaga ttctggacag gctggagtag cgccgtggcc
    15541 aacgaactct gcttccggaa tattcacctg gaccagccgt ggcgcctttc ggtgtaccag
    15601 ggcagtggcg gttacgagac gtggaaaaag atcctgcagg aaaagacgcc accggagaaa
    15661 attatcgacg agctgaagaa atcggcgctg cgcggccgtg gcggcgcggg tttctcgacg
    15721 ggcctgaaat ggtccttcat gccgaggaac acgccgggcc agaaatacat cgtgtgtaat
    15781 tccgacgagg gcgagccggg cacgttcaag gaccgcgata tcctgcgctt caacccgcat
    15841 gccctgatcg agggcatggc catcgccggg tatgccattg gcgcgaccgt gggttacaac
    15901 tacatccgcg gcgagttctg ggaaccttat gagcgtttcg aggccgcgct caaggaggcc
    15961 tatgcagccg gcctgctggg caagaatgtt ctcggttctg gcgtggattt cgacttatat
    16021 actcatctgg gcgccggggc ctatatctgc ggcgaagaga cggcgctgct cgaatccatc
    16081 gagggcaaga agggtcagcc gcgcttcaag ccgccgttcc cggccaatta cggtctgttc
    16141 ggacggccga ccactattaa taataccgag acgctggcct cggtcccgga cattatccgc
    16201 aacggcgccg actggtttct caatctggga aaaccgaata acggcggacc gaagctgttc
    16261 tcggtctcgg gccatgtcaa caagcccggc aactacgaag taccgctcgg cacgccgttc
    16321 gcaaagttgt tggaaatagc cggtggcgtg cgccacggcc atcatctcaa ggcagtcatc
    16381 cccggcggtt cctcggccaa cgtgctgccg gccgggatca tgatggaggt aaccatggat
    16441 tacgattcgc tcgccaaggc cggctccatg ctcggctcgg gcggcgtgat cgtgatggac
    16501 gacagtacct gcatggtgca cgcgctggcg cgcatcgcgc acttctatta cgaggaatcc
    16561 tgcggccagt gcacgccgtg ccgcgaaggc accggctgga tgtcgcgcgt gatccatcgc
    16621 atcgagcacg ggcagggccg gcctgaggac ctcgacatgc tcacggacgt cgcctccaat
    16681 atcgaggggc gcaccatctg cgcgctcggc gatgccgcgg cttggccggt aaaaagcttc
    16741 atcaagtact tccgcagcga attcgaatac cacatcgagc accgtcgttg catgccggga
    16801 acgcacggga gccacgcatg agcgccaatc cgaaagacgt caaaacgccg ccggtctccg
    16861 atacggtcac tattgaagtc gatggccgca agctgtcggc gcgcaagggt cagatgctga
    16921 tcgaggtgac cgacgcctcc ggcatttaca tcccgcgctt ctgttaccac aacaagctgt
    16981 cggtggcggc caactgccgc atgtgcctgg tggacgtgga aaaagcgccc aagccgctgc
    17041 cggcctgcgc cacgccggtg atggacggca tggtggtgca cacccgctcc gagaaagcgc
    17101 gcacggccca gaaaggcacg atggaatttc tgctgatcaa tcatccactc gattgcccga
    17161 tctgcgacca gggcggcgag tgcgaccttc aggaccaggc catggcctac ggcgtggacg
    17221 ccagccgctt ccacgagcgg ctgtcgatcg ccggcaattg ccggatgtgc ctggtcgagc
    17281 tcaagggctc gccgaagccg atcgcgagct gcgcctgggg cgtgcgtgac tgccggcccg
    17341 gtccaaaggg cgagccgccc gagg
//
LOCUS       DBNGZE010000221        11566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1280933, whole genome shotgun sequence.
ACCESSION   DBNGZE010000221 DBNGZE010000000
VERSION     DBNGZE010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1280933"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..777)
                     /locus_tag="AC16FV_05805"
     CDS             complement(43..777)
                     /locus_tag="AC16FV_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849606.1"
                     /translation="MRLKWVFAALVLANLGLWMWASWYREAPVEETRAARAPVAPEKM
                     RLFNEPGVKLIPRKAPPPANAKLTTGAAPACFHIGPFPDSGLVARAETRLNELQLTYS
                     RRSEETRLITGYQVYLPPLSSQKAAERKRQELTRLGFKDHALMQEEGMQNAISLGLFS
                     VEANAASRVRELAAKKVEARIQPFQQTRMRFWLDVAVKVPSETADKIRQIDWAAKDVQ
                     VQEITCPAGTSLPPDTPPAPRDNAST"
     gene            complement(774..1514)
                     /locus_tag="AC16FV_05810"
     CDS             complement(774..1514)
                     /locus_tag="AC16FV_05810"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR2849607.1"
                     /translation="MHLLIDLGNSRLKWAQYAAGLWRSDAALLDSEKNIEVLFDKAWG
                     KIAKPQRVIVCSVSSAERLQALGQWVRMHWSVTAYIVRSQMEQLGVKNLYHRPEQLGA
                     DRWAALIGARGLTNSAACVVDAGTAVTVEALSARGEFLGGAIFPGLRLLRDSLARGTE
                     AISAVTGNATDCLGRSTEDGVAAGTLFGLAGAVERLIDEYRRSLGEPMEIFLTGGDAP
                     ALAAHLHVPTAMIPDLVLKGLARIADSL"
     gene            complement(1528..2511)
                     /gene="birA"
                     /locus_tag="AC16FV_05815"
     CDS             complement(1528..2511)
                     /gene="birA"
                     /locus_tag="AC16FV_05815"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:NF008847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HIR2849608.1"
                     /translation="MTTRGEILRLLSDGGFHSGTDLGKKLGITRAAVCKNVHQLAQSG
                     LEVHRVTGRGYKLDTPLTLLDRSQFLKLMGGAAAEIRDRLHVLDEVDSTNRYLAEHVA
                     THMDIDGATCVTEAQSGGRGRRGRTWITTPYCNLMLSMAWRFPGGPSLVSGLSLAAGV
                     ALLRALEEYGVSGVGLKWPNDVLWDNRKLAGLLVDVQGEAAGPTQVILGVGINGYISP
                     QDAAHIDQPWIDLQGITGETTDRNRLAALVMRHLLGMFHLFAAKGFAPFRAEWQKRHL
                     FHGRRVRLIQGEREFTGTADGIDETGGLIIRHARNRQVFHSGEVSLRPARV"
     gene            complement(2508..2681)
                     /locus_tag="AC16FV_05820"
     CDS             complement(2508..2681)
                     /locus_tag="AC16FV_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2849609.1"
                     /translation="MHPRDRQLPADAILAVAKRQKCDLIFMASHGRRGLPGRKVVGER
                     SRRLNMTPVRMAP"
     gene            complement(2668..5370)
                     /locus_tag="AC16FV_05825"
     CDS             complement(2668..5370)
                     /locus_tag="AC16FV_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR2849610.1"
                     /translation="MATCFWHSMDVGLVLKELQVDPHRGLAPEEVQSRLSQYGPNELR
                     QEEKASPLALFFNQFKNILIVILLIATLLSALVGEYVDAVIIFVIIVFCAGLGFIQEY
                     RAERALEALKNMLSPTITVLRGGQEWEAPSKDLVPGDVLVLEAGDKIPADARLVEIHS
                     VHCDEAPLTGESIPVGKVLALSAEDASVGDRKNMVFTGTTVTYGRGKAVVTATGMATE
                     FGRIAEEVAAVTQEKTPLELRTEEIGKWLGFITLGICVLVVGISVAREAWAGTFRLDS
                     TLPIILFAIALAVAAVPEALAAIVTGALAIAMHEMAKRNALVRKMPAVETLGCTSVIC
                     SDKTGTLTKGEMTVRRLFVGGRMIEVSGVGYAPTGSLSSSLDDRALGLLLKGGVLASD
                     AVLAQDAGRWFVKGDPTEGALVVLAAKGRVRVQETRLACPRIEEFPFSSERKRMTTIH
                     TMEDGRRLAFTKGAPEVLLERCASTWAGDQAVPLGTDQRAKLAEANEAMAGDALRVLG
                     VAYKELPAENVCTEELAETDLTFLGLVGMMDPPRDEAIDAVKVCRQIQIKPVMITGDH
                     KLTAVAVAKEIGIFRDGDLVLTGDELAKMDEAALATVVERVTVYARVSPMDKLKIVSA
                     WKAKGQVVAMTGDGVNDAPALKHADVGVAMGISGTDVAKEAADIVLSDDNFASIVKAI
                     ERGRWIYDNIKKYLTYLLRANLTEVVVLGGVVVVMGPEFLPLLPAAILYINLATDGLP
                     ALALGVAPPDPDIMRRPPRDPKESVFSTEVRTLILLAVVIECPLFLWVFFAGFADIEH
                     ARTEIFLMFVLIELILALNFRSLRYSILEAPPHKWLLIAIGWELLLIAVLIQFDAVRE
                     SFGIAMPTFSDVAFVVALGFGVMGSIEIVKAVLRRRVSPASAST"
     gene            complement(5648..6382)
                     /locus_tag="AC16FV_05830"
     CDS             complement(5648..6382)
                     /locus_tag="AC16FV_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR2849611.1"
                     /translation="MIAWLSDAHTWMALFEIIGVNIVLSGDNAVVIALACRTLPTPQQ
                     RVAILAGSAGAIALRIVLTGFAAVLLSKPYLRLVGGILLIWIAIKLLLPETEADGDAQ
                     DKSGMFDAIKTIVIADFVMSLDNVLGVAAVAKGDLALLILGLVISMPLIVYGSTVIMK
                     LMERFPVVILLGAALLGYVAGDMAVSDGVVDPWIEAHVPLLHSLAAILSAILVVSAGK
                     LIAYRRSAVLASDAPEQDLEPPRESR"
     gene            complement(6448..7329)
                     /locus_tag="AC16FV_05835"
     CDS             complement(6448..7329)
                     /locus_tag="AC16FV_05835"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HIR2849612.1"
                     /translation="MKLGFIGLGIMGRPMALNLRRAGHALWVHGRRPVTMEVLVEAGA
                     QACHTVADVAASAEIIFIMVSDTPDVEHIVLGENGLIHRLRAGQMIVDMSTISASATR
                     RLAQELETRGVEMLDAPVSGGEIGAINANLSIMVGGKEKTFQRVKPLFDVLGKNVVHV
                     GDHGAGQVAKACNQIVAALTIEAVSEALTFARRNGVDAARVREALMGGFAGSKILEVH
                     GQRMLDNDFKPGFKVKLHQKDLRIVIEDAHKLGIGLPGAALVAQHLNALMGSGDGELD
                     SSAIVKVVERMSGMEVK"
     gene            complement(7503..8858)
                     /locus_tag="AC16FV_05840"
     CDS             complement(7503..8858)
                     /locus_tag="AC16FV_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR2849613.1"
                     /translation="MGDLHHLGVKEALHGLSEGRFTCEDLVRDCLVQIARLDPKIEAW
                     VWLKSEAALEHAREADRHRHAGKLGALLGIPVGVKDIIDAQGVPTRMGSPAFEDYLPS
                     MSARVVRRLDEAGAVMLGKTVTAELAYYVPGKTRNPWNPEHTPGGSSSGSAAAVAARF
                     VPAAIGTQTNGSIIRPAAFCGVVGYKPSAGLISRSGILKFSHTLDQVGVFARNVPDAA
                     VVASALIGHTQDDPDSLSDFALVPHDLDPKPLFQPPRLAAVRTPVWSLAETDQQENFT
                     RSIAALRMIGTVIETVMLPDAFARAHDVHRTILPYEGARAFAHLQTQQRDRLSTEINL
                     LIDDGLQIPEAAYHAALESRTRLRGELGEFMYRYDAVLTPPARGEAPAMLTNTGDPVF
                     CTLWTLCGAPAITLPAGLGQHGLPLGLQLVGGYLQDARLLQVAQWCSLQLGFRHSPPG
                     V"
     gene            complement(8859..9830)
                     /locus_tag="AC16FV_05845"
     CDS             complement(8859..9830)
                     /locus_tag="AC16FV_05845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR2849614.1"
                     /translation="MSRPRVLVTREVFDDVLEYLAHHFDVTPNQADTPMSPTALAQAL
                     ADKEGALTTLSERVDAALLAQCPKLRAVCNIAVGFNNIDLKACQARGVMATNTPGVLD
                     DSTADFTWALILATARRVTEAEQTLRAGKWTGWKLKQFLGVDVHHATLGILGMGRIGQ
                     AVARRARGFDMTVLYHNSRRLSAEVEKSCRAAYVDMNELLARADILTIHVPYSPATHH
                     IIDAAQLAKMKPGASLINAARGGVVDDAALIEALKCGTIAAAGLDVFENEPALHPDFL
                     KLANVVLTPHIASSSEATRRRMAMLAAENLVAALTTGKPPNLLHPPA"
     gene            complement(9846..>11566)
                     /locus_tag="AC16FV_05850"
     CDS             complement(9846..>11566)
                     /locus_tag="AC16FV_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR2849615.1"
                     /translation="VLIAIPLFLFMGYLVERAQLIDRLFHSLHLATARVPGSLAVATI
                     VTCAIFATATGIIGAVVTLMGLLALPAMLKAGYNVKFAAGAITAGGCLGILIPPSVLL
                     IVYGAVAGVSVVQLYAGAFLPGFMLAGLYIGYIILFAKWKPHLMPPLAESERRVPLPP
                     LSQRLAPHHRNALMGLWRALTGGVGAGVAKHTVAGQFIVALLPALAMVAILGVTYRVA
                     TAPVIQASTTGLVEAGGLVAAPVEEQEPAGLIGAPDAEADKSGLQEPPAEDVKEVAAP
                     AAAEAAKPEVVEKAAVVEAERLPVPTWFWVVCGSAIALVVVRYRRFNWQRLEIFKMLL
                     TSFFPLALMILAVLGSIVVGLATPSEAAAVGAFGGFILAGAYRFIAYWQEGKSSGKNT
                     GKVIWSTVKELGVIVKESSFLTAKTSAMVCWLFVGSGIFAAAFALLGGQELIEKWVLS
                     LDMTPLQFMILAQFIIFILGWPLEWTEIIIIFMPIFSPLLPHFHIDPLFFGLLVALNL
                     QTAFLSPPVAMAAFYLKGVSPPHVTLNQIFAGMMPFMGIQLIALILLYMFPQIGLWLP
                     QVFYNQ"
BASE COUNT         2261 a         3774 c         3459 g         2072 t
ORIGIN      
        1 aaacgcggca ttgtaagtat acccccgtga ccgggcaacc actcaggtcg acgcattatc
       61 gcgcggcgca ggcggcgtgt ccggcggcaa gctggtaccg gccgggcagg tgatttcctg
      121 tacctgaacg tctttcgcgg cccagtcaat ctgtctgatc ttgtccgccg tttccgatgg
      181 aaccttaacg gccacatcca gccagaaacg catgcgcgtc tgctggaacg gctgaatcct
      241 ggcctccact tttttggcgg ccagctcacg cacgcggctt gcggcattgg cctcgacgct
      301 gaacaaaccg agcgagatcg cgttttgcat accttcttcc tgcatcaggg catgatcctt
      361 gaacccgaga cgggtgagtt cctggcgttt gcgctccgcg gctttctggg acgacagcgg
      421 cgggagatac acttggtaac cggtgatcag tcgggtttcc tcggaacggc gtgaataagt
      481 gagctgaagt tcgttcaaac gggtctccgc tctcgcgacg agaccggaat ccggaaacgg
      541 gccgatatga aaacaggcag gtgcggcacc tgtcgttaat ttcgcattgg ccggcggtgg
      601 cgccttgcgc ggtatcaact tcacgcccgg ttcgttgaac agacgcattt tttccggcgc
      661 caccggcgcg cgcgccgcgc gtgtttcctc caccggcgct tccctgtacc aactggccca
      721 catccagagc ccgaggttgg ccagcaccaa cgcggcgaaa acccatttga gtctcatagg
      781 ctatccgcga tccgtgccag acctttcagc accaggtccg gtatcatggc ggtcggcaca
      841 tgcagatgcg cagccagcgc cggggcatcg ccaccggtga gaaaaatctc cattggttcg
      901 cccaagcttc gccggtattc atcaatcaag cgctccaccg cgcccgccag accgaacagg
      961 gtgccggctg cgacgccatc ctcggtggaa cgaccgagac agtccgttgc gttgcccgtg
     1021 acggcggaaa tagcctccgt gccgcgcgcc aggctgtcgc gcagcagacg caggcccgga
     1081 aaaatcgcgc cgccgagaaa ttcgcccctg gccgacagcg cttccaccgt cacggccgta
     1141 ccggcatcca ccacacatgc cgctgaattg gtcaggccac gcgcaccaat cagggcggcc
     1201 cagcggtcgg cgcccaattg ttccggccgg tgataaagat ttttcacgcc gagctgttcc
     1261 atttgcgagc gcacgatgta tgccgtgacg gaccagtgca tccgcaccca ttgaccgagc
     1321 gcctgcaatc gctcggcgct ggaaacactg catacgatta ctcgctgcgg tttcgcaatt
     1381 tttccccacg ccttatcgaa caaaacttcg atgttttttt cactgtcgag cagggcagca
     1441 tcgctgcgcc acagaccggc ggcgtattgc gcccacttca ggcggctgtt accgagatcg
     1501 attagcaggt gcatacgagg aatgccgcta gacgcgggcg ggcctcaggc tgacctcacc
     1561 ggaatgaaac acctgccggt tcctggcgtg acggatgatc agcccaccgg tttcgtcgat
     1621 gccatcggcg gtgccggtga actcgcgctc gccctggatc aggcgcacgc gccggccgtg
     1681 gaacagatgc cgcttctgcc attccgcgcg gaacggcgcg aagcccttgg ccgcgaacag
     1741 atgaaacatg cccagcagat gacgcatgac caacgccgcc aggcgattgc ggtcggtggt
     1801 ttcacccgtg atgccctgca ggtcgatcca cggctggtca atgtgcgcgg cgtcctgcgg
     1861 gctgatgtac ccattgatgc ccacgccgag gataacctgt gtcggtcccg ccgcctcgcc
     1921 ctggacatcg accaacaggc cagcgagttt gcggttatcc cacagcacgt cgttcggcca
     1981 cttgagcccc acgccggaca cgccgtattc ctccagcgcg cgcaacaacg caacaccggc
     2041 cgccaggctc aggcccgaaa ccagacttgg tccgccggga aagcgccagg ccatggacaa
     2101 catgagattg cagtatggcg tggtgatcca ggtgcgcccg cgccggccgc gaccgcccga
     2161 ctgggcttcg gtcacgcagg tcgcgccgtc gatgtccata tgcgtcgcga catgttcggc
     2221 gagataacga ttggtggaat ccacttcgtc caggacgtgc aggcggtcgc ggatttccgc
     2281 cgccgcgccg cccatcaact tcaggaattg ggaacgatcc agcaacgtca gtggcgtgtc
     2341 gagcttgtag ccgcggccgg tcacgcggtg aacctcgagg cccgattgcg cgagctgatg
     2401 gacattcttg caaaccgcgg cgcgcgtaat gccgagtttt ttgcccagat cggtgcccga
     2461 atgaaaaccg ccgtcggaga gcagcctgag aatttcaccg cgcgtcgtca tggcgccatt
     2521 ctaacagggg tcatattgag gcgacgagaa cgctcgccaa ccaccttcct gcccggcaac
     2581 ccgcggcggc cgtgcgaggc catgaaaata agatcgcact tctgccgctt cgccacggcg
     2641 aggatggcgt cggcgggaag ttgtcggtca cgtggatgca ctcgcaggcg acacccgcct
     2701 ccgcagcacc gctttgacga tctcgatcga acccatcacg ccgaatccaa gtgccactac
     2761 gaacgccacg tcggagaagg tcggcatggc gataccgaac gattcgcgaa cggcgtcaaa
     2821 ctgaatcagg acagcgatca gaagcagctc ccacccgatg gcgatcagga gccacttgtg
     2881 gggcggcgcc tccaggatgc tgtaacgcag cgagcggaag ttgagggcga ggatcaactc
     2941 gatcaggacg aacatgagga agatctcagt acgagcgtgc tcgatatcgg cgaaacctgc
     3001 aaagaaaacc cagagaaaga gcggacattc gatgaccacg gcaagcagaa tgagcgtgcg
     3061 cacctcggtt gagaaaacgc tctccttcgg atcgcgagga ggacgccgca tgatgtcggg
     3121 gtcgggaggc gccacgccca acgcaagtgc cgggagcccg tcagtcgcca gattgatgta
     3181 gaggatggca gccggcagca gcggcaggaa ttcaggtccc atgaccacca ccaccccgcc
     3241 cagcaccacg acctcggtca gattcgctct gagcagatag gtgagatact ttttgatgtt
     3301 gtcgtagatc caccgtccgc gctcgatggc cttgacaata ctggcgaagt tgtcgtcact
     3361 gaggacgata tcggcggcct ccttggccac gtcggtcccc gagatcccca tcgccacccc
     3421 cacgtcggca tgcttgaggg cgggggcgtc gttgacgccg tcgccggtca tagcgacaac
     3481 ctgccccttc gccttccatg cgctcacgat cttcagcttg tccatcggcg agaccctggc
     3541 atagacggtc acgcgctcca ccacagtcgc cagcgctgcc tcgtccatct tggcgagctc
     3601 gtcacccgta aggaccaggt ctccgtcccg gaagatcccg atttccttcg ccacggccac
     3661 ggcggtgagt ttgtgatccc cagtaatcat taccggtttg atttggatct gccgacagac
     3721 cttgacggca tcgatcgcct catcccgggg cggatccatc atccccacga ggccgaggaa
     3781 ggtcaggtcg gtctcggcca actcctccgt gcacacgttc tcggccggca gttccttgta
     3841 ggcgacgccc agaacgcgaa gggcatcgcc ggccatcgcc tcgttggcct cagccagctt
     3901 cgcccgctga tcggtcccga gcgggacggc ttggtcccca gcccacgtag aagcgcagcg
     3961 ttccagcagc acctccggcg cccctttggt gaaggcaagc cgccgcccgt cctccatcgt
     4021 gtggatggta gtcatgcgct tgcgttcgga actgaacgga aactcctcga tgcgcggaca
     4081 cgcgagccgg gtttcctgga ctcgtaccct gcccttggcc gccagcacta cgagggcccc
     4141 ttcggtcgga tcgcctttga cgaaccatcg ccccgcatcc tgggccaaca cggcgtcgct
     4201 ggcgaggaca ccgcctttga gcagcaagcc gagcgcgcga tcgtcgagcg agctgctcaa
     4261 gctaccggtc ggggcatacc ccactccgct cacctcgatc atccggccgc cgacgaacag
     4321 ccggcgtacg gtcatctcac ccttggtaag cgtaccggtc ttgtccgagc agatcacact
     4381 ggtgcagccg agggtttcga cggcgggcat cttgcggacc aacgcgttgc gcttggccat
     4441 ctcgtgcatg gcgatggcca gcgcacccgt gacgatggcg gcgagcgcct cgggtaccgc
     4501 cgcaactgcc agggcgatcg cgaacaggat aatcggcagc gtagagtcga gccggaaagt
     4561 gcctgcccat gcctcccgcg cgacgctgat gcccacgacc agcacgcaga tgccgagcgt
     4621 gatgaacccc agccacttgc cgatttcctc ggttcgcagc tcaagcgggg ttttctcctg
     4681 cgtcaccgcc gccacctcct ccgcgatccg accgaattcg gtcgccatcc cggttgcggt
     4741 caccactgct ttgccccgcc cgtacgtcac cgtggtgccg gtgaagacca tgttcttgcg
     4801 atcgcccacg gatgcgtcct ccgctgacag cgcaagtacc ttgccaacag gaatcgactc
     4861 cccggtaagc ggcgcctcat cacaatgcac cgagtggatc tcgaccagcc tggcatcggc
     4921 aggaatcttg tcgccggcct cgaggaccag gacatcgccc ggcacgaggt ctttcgacgg
     4981 tgcctcccac tcctgtccac ccctcagaac tgtgattgtc gggctgagca tgttcttcag
     5041 ggcttccagc gcgcgctcgg cacggtactc ctgaatgaag ccgagaccgg cgcaaaacac
     5101 aatgatcacg aagataatga ccgcgtccac gtactcgccg accaaggcgg aaagaagggt
     5161 ggcgatcagc agaatgacga tgaggatgtt tttgaactgg ttgaagaaga gcgcgagcgg
     5221 cgaggccttc tcctcctgcc taagctcatt gggaccgtat tggctgaggc ggctttgcac
     5281 ctcttccggt gcaaggcccc ggtgcgggtc gacttgcagt tctttcagga cgagaccaac
     5341 atccatcgaa tgccagaagc acgtagccat taggtcctcc cgacgttatc catcagtagt
     5401 gcgtaatgcg gtgtctgtct tgctccgccg cagccttagc ggctcaccag aagcatcgcc
     5461 tctccaaaaa aaatgcgcca ggacgaaaac gaccgggagc gtggccgcag tggtggcgga
     5521 tgcatttgca caccgccgcg cgggtgatac cgagttttta tccgaggttt gtgcccgaat
     5581 gaaaactgcc gtcggacagc agcctgagga tttcggcgcg cgtgctcatg cgggaattgt
     5641 aacccgatta tcgggattcc cgtggtggtt cgaggtcctg ctccggcgcg tcgctggcaa
     5701 gcaccgcgct gcggcggtac gcgattaatt ttccggccga taccaccagg atggcgctga
     5761 gaatggcagc cagcgagtgc agcagcggca catgcgcctc gatccaggga tccaccaccc
     5821 catcggagac ggccatgtca cccgcgacat aaccgagaag cgcggcgccc aacaagatca
     5881 cgaccggaaa gcgttccatc agcttcatga ttaccgtgct gccgtagacg atcaaaggca
     5941 tgctgatcac aagccccagg atcagcaaag ccagatcccc cttggcaacg gccgccacgc
     6001 cgaggacatt gtccaggctc atcacaaaat ccgcgatcac gatggttttg atggcgtcga
     6061 acatgccgga tttgtcctgc gcgtcaccat cggcttccgt ttccggcagc aacagcttga
     6121 tggcaatcca gataagcaaa atacccccga cgagccttag ataaggcttg ctaagcagta
     6181 ccgcggcgaa tccggtcaat actatccgca atgcaatcgc gccagcgctg ccggcaagaa
     6241 tcgccacacg ttgctgcggc gtcggcaaag tgcggcaagc cagggcgatc acgacggcat
     6301 tgtcaccgct caagacgatg ttgacgccga tgatctcgaa caatgccatc caggtatgcg
     6361 catcactcag ccaagcaatc attccaccct ccggtcctgt cttcggtccg ccgtatcaga
     6421 tggttaaata gtaacgccgg tcattcgcta tttcacttcc atcccggaca ttcgctccac
     6481 gactttgaca atcgcgctgg aatccagctc accgtcgccg ctgcccatga gcgcgttcag
     6541 gtgctgcgcc accagcgccg cgcccggcag gccgatgccc agtttgtgtg cgtcctcgat
     6601 gacgatgcgc aggtccttct ggtgcagctt caccttgaag ccgggtttga agtcgttgtc
     6661 gagcatgcgc tggccgtgca cttccagaat cttgcttcca gcgaatcccc ccatcagcgc
     6721 ctcgcgcacc cgggctgcgt ccacgccgtt cctgcgcgcg aaggtcagcg cctcgctcac
     6781 cgcctcgatc gtgagcgccg ccacgatctg attgcaggcc ttggccacct gaccggcgcc
     6841 gtgatcgccc acatgcacta cgttcttgcc gagcacgtcg aacagcggtt tcacgcgctg
     6901 gaaggttttt tccttgcccc cgaccatgat ggacaggttg gcattgatgg caccgatctc
     6961 gccacctgag accggcgcat ccagcatctc gacaccgcgc gtctccagct cctgcgcgag
     7021 gcggcgcgtg gccgaggcag agatggtgct catatccacg atcatctgcc cggcgcgcag
     7081 gcgatgaatc aggccgttct cgccgagcac gatatgttcc acatcgggcg tgtccgagac
     7141 catgatgaaa atgatctcgg cgctggcggc gacatccgca accgtgtggc aggcctgtgc
     7201 gccggcctcc accagtacct ccatcgtcac cggccggcgc ccgtgtaccc acagcgcgtg
     7261 cccggcccgg cgcaggttca gcgccatggg ccggcccatg atcccgagcc cgatgaatcc
     7321 cagtttcatc gccattgatg cacgcctcct tggaattctt aacaaacaat tttgacagga
     7381 tttacaggat taaaagagga ttaacaggat ttttgtttct tgaatcttca atcaatcctg
     7441 taaatcctga gtaatcctgt taatcctgtc tattttcatt ttgctggcgg ctactcttca
     7501 attcaaactc ccggcggact gtgccggaac ccaagctgca aactgcacca ctgcgccacc
     7561 tgcaacaggc gcgcgtcctg cagataaccg ccgaccagtt gcaatccgag cggcaggccg
     7621 tgctggccca gacccgcagg cagcgtaatg gccggtgcgc cgcacaacgt ccagagggta
     7681 cagaacaccg ggtcgccggt attcgtcagc atggcgggcg cttcgccgcg cgccggcggc
     7741 gtcaacacgg cgtcgtaacg atacatgaat tctcccaatt cgccacgcaa acgggtgcgg
     7801 ctttccagcg ccgcgtgata cgcagcctcc ggaatctgca aaccgtcatc gatcaagaga
     7861 ttgatctcgg tgctgaggcg gtcacgttgc tgcgtttgca gatgagcaaa cgcccgcgcg
     7921 ccttcgtagg gcaggatcgt gcgatggaca tcgtgcgccc gggcgaaggc atcgggaagc
     7981 atgacggttt cgatgaccgt gccgatcatg cgcaacgcgg cgatactccg ggtgaaattt
     8041 tcctgctggt cggtctccgc cagcgaccag accggcgtgc gcaccgccgc cagtcgcggc
     8101 ggctgaaaca acggcttggg gtcgaggtca tgcggcacca gagcgaagtc agacaggctg
     8161 tcggggtcat cctgcgtgtg accgatcagg gcactcgcca ccaccgcggc atccggcacg
     8221 ttgcgcgcga acacgccgac ctgatccagt gtatggctga acttgagtat tccggagcgc
     8281 gatataagcc cggcgctggg tttgtaaccg accacgccgc agaacgcggc gggacggatg
     8341 atggagccgt tggtctgggt gccgatcgcg gccggaacga atcgcgccgc caccgccgcg
     8401 gccgaaccgc tcgaagaacc gcccggcgtg tgttccgggt tccacgggtt gcgtgtttta
     8461 ccgggcacat aataggccag ctccgccgtc acggttttac ccagcatcac cgctccggcc
     8521 tcgtccagac gccgcaccac gcgcgcggac atcgacggca ggtaatcctc gaaagcgggc
     8581 gagcccatgc gtgtgggcac gccctgcgca tcgataatat ccttcacgcc caccggaatg
     8641 cccagcagcg cgcccaactt gccggcgtgc cggtgacgat cagcctcgcg cgcgtgctcc
     8701 agcgctgcct cggacttgag ccacacccag gcttcgatct tgggatcgag gcgcgcgatc
     8761 tgcaccagac aatcgcgcac caggtcttcg caggtgaagc ggccctcgga caggccgtgc
     8821 agggcttcct tcacacccag atgatgcagg tcccccattc aggcgggcgg atgcaggaga
     8881 ttaggcggct tgcccgtggt cagcgccgcc accagattct ctgccgccag catcgccatg
     8941 cggcggcgcg tagcctcgga ggaactggcg atgtgaggcg tcaggacgac attggcgagt
     9001 ttcagaaaat ccggatgcag cgccggttcg ttctcgaata catcgaggcc ggcggcggcg
     9061 atggtgccgc acttcagcgc ctcgatgagc gccgcgtcat cgaccacgcc gccgcgcgcg
     9121 gcgttgatga gactggcgcc gggtttcatt ttcgcgagtt gtgccgcgtc aataatatga
     9181 tgagtggccg gagagtacgg tacatgaatg gtaagaatat cggcgcgcgc gagcagctca
     9241 ttcatatcta catacgccgc gcggcagctt ttttccactt ccgccgacag acggcgcgaa
     9301 ttgtggtaca gcacggtcat gtcgaaaccg cgggcgcggc gcgccaccgc ctggccgatg
     9361 cgacccatcc cgagaattcc cagcgtggca tggtgcacgt ccacgccgag gaattgtttc
     9421 agcttccagc cggtccattt tccggcacgc agggtttgtt ccgcctcggt cacgcggcgc
     9481 gccgtggcca ggatcagcgc ccaggtgaaa tcggccgtcg agtcatccag cacgcccggg
     9541 gtgttggtgg ccatgacgcc gcgcgcctga caggccttga gatcgatgtt gttgaaaccg
     9601 acggcgatgt tgcacaccgc cctgagtttc ggacactgcg ccagcagcgc ggcatcgacg
     9661 cgttcgctca gggtcgttag cgcgccctcc ttgtccgcga gcgcctgcgc cagcgccgtc
     9721 gggctcatgg gcgtgtccgc ttggttcggc gtgacgtcga aatggtgcgc gagatactcg
     9781 agcacatcgt cgaacacttc gcgcgtcacc aagactcggg gccgactcat gccgaaacat
     9841 ccctgttatt gattataaaa cacctgcggc aaccacaggc cgatctgcgg gaacatgtag
     9901 agcaggatca gcgcgatcag ctggataccc atgaacggca tcatgccggc gaagatctgg
     9961 ttgagtgtga catgcggcgg cgagacgcct ttgagataga acgccgccat tgccaccggc
    10021 ggcgacagga acgccgtctg gaggttcagc gccaccagca gcccgaaaaa cagcggatcg
    10081 atatggaagt gcggcaagag cggactgaag atcggcatga agatgatgat gatctcggtc
    10141 cactcgagcg gccagccgag gatgaagatg atgaactggg cgaggatcat gaactgcagc
    10201 ggcgtcatgt cgagcgacag tacccatttc tcgatcagct cctgcccgcc gagcagcgcg
    10261 aatgccgccg cgaaaatacc ggacccgacg aacagccagc acaccatcgc gctcgtcttg
    10321 gcggtgagga aggacgactc cttgacgatg acgccgagtt ccttgaccgt gctccagatc
    10381 accttgccgg tattcttgcc gctggacttc ccctcctgcc aataggcgat gaaacggtag
    10441 gcccccgcca gaatgaaccc gccgaacgca ccgaccgccg ccgcctcgga aggtgtcgcc
    10501 agaccgacca cgatcgagcc gagcaccgcg aggatcatca gcgccagcgg gaagaacgag
    10561 gtgagcagca tcttgaatat ctcgagccgc tgccagttga agcgccggta gcggacgacg
    10621 accagggcta tggcgctgcc gcaaacgacc cagaaccatg tcggaaccgg gagccgttcg
    10681 gcttcgacga cggccgcctt ttccacgact tcgggcttcg ccgcctccgc cgcggctggc
    10741 gcagcaactt ctttcacgtc ttcggcggga ggttcctgga gccctgattt atccgcttcc
    10801 gcatccggcg ctccgatgag gcccgccggt tcctgttcct ctacgggcgc tgccaccagg
    10861 ccgcccgcct cgaccaaccc ggtggtgctg gcttggatca ccggcgcggt cgctacccga
    10921 taggtgacgc ccaagatggc gaccatggca agcgccggga gcagcgcgac gataaactgc
    10981 ccggctacgg tgtgtttcgc gacgccggcg ccaacgcctc cggtgagtgc acgccacagc
    11041 cccatcaacg cgttgcgatg atgcggcgcc aatctttgcg acaaaggtgg aagcggcacg
    11101 cgccgctcgc tctcagccag cggaggcatc agatgcggct tccacttggc gaacaagatg
    11161 atgtacccga tgtaaagtcc cgcgagcatg aagccgggca ggaaggcgcc ggcgtacagc
    11221 tggaccacgg atacccccgc cacggcgcca taaacaatca gcagcacgga gggtgggatc
    11281 aggatgccga ggcagccgcc tgcggtgatc gcgcccgcgg caaacttgac gttgtagccg
    11341 gccttgagca tcgccggtaa cgcgagcagg cccatcagcg tcaccaccgc gccgatgatc
    11401 ccggtggcgg tagcgaagat cgcgcaggtg acgatagtcg ccaccgcgag cgagcccggc
    11461 acgcgcgcgg tggcgagatg caggctgtgg aacagccggt cgatcaactg cgcccgctcg
    11521 acgagatagc ccatgaacag gaacagcggg atcgcgatca gcacat
//
LOCUS       DBNGZE010000222         5064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_378144, whole genome shotgun sequence.
ACCESSION   DBNGZE010000222 DBNGZE010000000
VERSION     DBNGZE010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_378144"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC16FV_05855"
     CDS             <1..568
                     /locus_tag="AC16FV_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HIR2849616.1"
                     /translation="GICSAAQAETVYIAERVRVGLRADMDETSAVVKTVETGAALEVV
                     ERQEKLVRVRDLQGTEGWIEARYLTPEPPARLQLAKLQEELAKSRAQTAEAQAQLKKT
                     QSTLAEQTDKLKELEKNSADRPAPAPVAPVAAKSPPVKPSGVDAGFNFNYLWLGISFA
                     MLGIGFAAGVKWLRESIRKRSGGMYLRV"
     gene            572..1132
                     /locus_tag="AC16FV_05860"
     CDS             572..1132
                     /locus_tag="AC16FV_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR2849617.1"
                     /translation="MERVKAILVITLAVAGLSAAQAGTLYKWVDSQGRVSYRDQPPPE
                     GSGYRVEEKNLGSGKKAEVDDTLEKIVEKFPVILYSVPVCGSCDLARAYLQKRKVPFS
                     EQNLENNAELQQTLKKKIGSLSAPTIMVGEKVMKGYVESILEGELDAAGYPKIESSGS
                     DKEEDKGQENTSASGDSGYQPARRRY"
     gene            1166..1933
                     /gene="xth"
                     /locus_tag="AC16FV_05865"
     CDS             1166..1933
                     /gene="xth"
                     /locus_tag="AC16FV_05865"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR2849618.1"
                     /translation="MKLATWNVNSIRVRLPQVLEWLKKEKPDVLCLQETKITDGEFPV
                     AAFRDIGYQAVYTGQKTYNGVATLSRAPAADVISVLPGADGDQKRLLATVVKGMRVIN
                     VYIPNGEEVGSEKYSYKLSWLKALEKFIARELKSHPRLVLLGDYNIAPEERDVHDPKR
                     WEGRVLFSDKERAAFQRLAQNGLTDVFRRFDQPEKSFSWWDYRAGAFHRNHGLRIDHI
                     LCSPTLASECRGCRIDIEPRKHERPSDHAPVIAEFGL"
     gene            complement(1991..2764)
                     /locus_tag="AC16FV_05870"
     CDS             complement(1991..2764)
                     /locus_tag="AC16FV_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2849619.1"
                     /translation="MLFSLTAVAEEEYILSAPPRETGENETEVYQPIADYLSKATGKK
                     IVYRHSDNWLSYQDGMRKGTYDLVFDGPHFVSWRMAHLKHVPLVKLPGNLAFVVAVRK
                     DNNRINDIKDLAGRSVCGMAPPNLATLTLYSQFGNPARQPLITEVRSFELAYQNVVNG
                     KCVAAVMRDQMLDKLNKDKDAVRVIFRSPGVPNQAFSAGPRFSPEDRNKIAESLLSPE
                     ARKQLPQFFGRYSKDKDLISARDTEFRDLLVLLKDSWGF"
     gene            3104..3943
                     /locus_tag="AC16FV_05875"
     CDS             3104..3943
                     /locus_tag="AC16FV_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR2849620.1"
                     /translation="MLATAIIVFREVLEAALVIGIVLAATQGLLRRGTWVSAGVVAGV
                     VGAGLVALFADVISSAFAGIGQEVFNAGVLLFAVIMLGWHNVWMKRHGREMAQHMRDV
                     GQAVRLGSRSMYMLAVVIGLAVLREGSEVVLFLYGIAVAQQDSPALMLSGGLLGLLAG
                     AMVGLLMYRGLLRISSRHLFTVTTWMITLLAAGMAAQAAGFLTQADLLPPLVQSMWDT
                     SGIVAQNSVFGRVLQTLIGYADRPSGMQVLFYAATLAGIAVLMRVYGGALSRKPVAVT
                     AEI"
     gene            4042..4605
                     /locus_tag="AC16FV_05880"
     CDS             4042..4605
                     /locus_tag="AC16FV_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HIR2849621.1"
                     /translation="MRSCNKRKLAIGAFLALTGMPGIAMAVEYPIGTPQQQHGMEIAA
                     VYLQPIEMEPEGMMRKAVESDVHLEADIHALSSNPNGFDEGAWVPSLLIKYELTKAGS
                     DWKASGDFMPMVANDGPHYGDNVKLGGPGKYHLKYTIYAPNAPENPYGKHFGRHTDRL
                     TGVRPWFKPFEVEYDFTFAGIGKKGGY"
     gene            4605..>5064
                     /locus_tag="AC16FV_05885"
     CDS             4605..>5064
                     /locus_tag="AC16FV_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849622.1"
                     /translation="MKPYSLCSFVLPYDEKPYSLRSFILTYRMRAIKSLALLAIAAYA
                     GAAVGAEPRSASEEIKELKERVHKLEQEKAREEKALSGERVSEEEPEIVTRLKAVEFQ
                     SLGMQKQARTIESLEGITAGVSFTTVAQHAGGSATTDGSSESQWNYRADVS"
BASE COUNT         1073 a         1407 c         1549 g         1035 t
ORIGIN      
        1 gggaatctgt tccgctgccc aggcggaaac ggtatacatc gccgagcgtg tccgcgtagg
       61 gttgcgcgcc gacatggatg aaaccagcgc ggtggtgaaa acggttgaaa ccggcgcggc
      121 gctggaagtg gttgagcgac aggaaaagct ggtgcgcgtg cgcgatctcc agggtaccga
      181 gggctggatc gaggcgcgct atctcacgcc cgagcccccg gcgcgtctgc aactcgccaa
      241 acttcaggag gaactggcca agagccgcgc gcagacagcc gaagcacagg cacagctcaa
      301 gaagacccaa tcgacccttg cggagcagac cgacaagctg aaggagctgg aaaagaacag
      361 cgctgacaga ccggcgcccg ctcccgttgc gccggtcgcc gccaaaagcc cgcccgtcaa
      421 gccaagcggg gtggatgccg gttttaactt caattatcta tggctcggga tttcctttgc
      481 tatgcttgga attggattcg ccgccggcgt gaaatggctg cgcgaatcca tccgcaaacg
      541 atcgggtggc atgtatctga gggtttgacc catggaacgc gttaaagcaa ttctggttat
      601 cacgctggct gttgccggct tgtccgcggc gcaggcagga acattatata aatgggtgga
      661 cagccaggga cgggtgtcgt atcgcgacca gccgccgccc gagggttccg gttatcgcgt
      721 cgaagaaaaa aatcttggct ctggcaagaa agccgaagtg gacgatacgc tcgaaaagat
      781 cgtcgaaaaa ttcccggtca tcctttattc cgttcccgtc tgcggctcct gcgatctcgc
      841 gcgtgcatat ctgcagaagc gcaaggtgcc gtttagcgag cagaatctcg aaaataacgc
      901 agaactgcaa cagacgctga aaaagaagat cggttcgctg tcggctccca ccatcatggt
      961 cggcgaaaag gtcatgaaag gttacgtgga atccattctc gagggcgagc tggacgccgc
     1021 cggctatcca aaaatcgaat cgtccggatc cgacaaggaa gaagacaaag gtcaggagaa
     1081 cacctctgct tcgggagatt ccggctacca gcccgcccgc cgccgttatt gaaccggcct
     1141 gaatccaggc cgtaaactcc ctgtcatgaa actggcgaca tggaatgtca attccatccg
     1201 tgtgcgtttg ccccaggtgc tggagtggct gaaaaaagaa aagccggatg tgttatgcct
     1261 gcaggaaacc aagatcacag acggggaatt tcccgtcgca gcattccgcg acatcggtta
     1321 tcaggccgtt tacaccggcc agaagaccta taacggtgtc gctaccctga gccgtgcccc
     1381 ggcggctgat gtcattagcg tgctacccgg cgccgacggc gaccagaaac gtttgctcgc
     1441 cacggtggtg aagggcatgc gcgtgatcaa cgtgtacata ccgaacggcg aggaagtcgg
     1501 gtcggaaaag tattcctaca aattatcctg gctcaaggcg ctcgagaaat tcatcgccag
     1561 ggagctcaaa agccacccgc gcctggtgct actgggcgat tacaacatcg cgccggaaga
     1621 gcgcgacgtg cacgacccca agcgctggga aggccgcgtg cttttcagcg acaaggagcg
     1681 cgcggcgttc caacggctcg cccaaaacgg actgacggac gtgttccgcc gtttcgatca
     1741 gccggaaaaa agcttcagct ggtgggatta ccgcgccggg gcatttcacc gcaaccacgg
     1801 cctgcgcatc gatcatattt tgtgcagccc cacgttggcg tcggagtgtc gtggttgccg
     1861 catcgacatc gagccgcgca aacacgaacg cccctccgat catgctccgg tgatcgcgga
     1921 gttcggtcta tagcgtattg atcggctgtt taagccgtga tgatcaaggt agcttgatca
     1981 tcagcgcaac ctagaatccc cacgaatctt tcagcaggac cagcagatca cggaattcgg
     2041 tgtcacgggc tgaaatcaaa tccttgtcct tgctgtaccg gccgaagaac tggggaagct
     2101 gtttgcgtgc ctccggggag agcagggatt cggcgatctt gttccgatct tccggggaaa
     2161 agcgtggtcc cgcggaaaac gcctgattcg gcacgccggg actccggaaa atcaccctga
     2221 cggcgtcttt gtccttgttc agtttatcga gcatctggtc acgcatcacc gcggccacgc
     2281 acttgccgtt aacgacgttc tgataggcca gctcgaaact ccgtacctcc gtgatcagcg
     2341 gctggcgagc cggattgccg aactgactgt agagtgtcag tgtggccaga ttgggcggtg
     2401 ccatgccgca cacggaacga cccgctaggt ctttaatgtc gttgatgcga ttgttgtctt
     2461 ttcggacggc gaccacgaac gcgaggtttc cgggcagctt gaccaacggc acgtgtttca
     2521 ggtgcgccat gcgccatgaa acaaagtgcg gtccatcaaa aacaagatcg taagttccct
     2581 tgcgcatccc gtcctgatag gacagccagt tgtccgaatg ccggtagacg atctttttcc
     2641 ccgtcgcttt cgataggtaa tccgcaatcg gctgatagac ctcggtttcg ttttcgcctg
     2701 tttcacgcgg cggcgcgctc aggatgtatt cctcttcggc gacagcggtt aaggaaaaaa
     2761 gcatggtgga cagcagcgca cacgtcagaa gattttttgc aggcatggaa tgtaactgaa
     2821 taaggcgttg gatggcgcgg ttaagacgct aatggcggca gccctattcc gtgggatccc
     2881 ctgtcgtgca gagaggaatg tggggccgcc ctgcgtggcg gtgtttctcg aggagcctgc
     2941 gtgatacacc aaatgcctga attcaggctg tttgccgccc tccggagggt ctgcttgaca
     3001 gcctggttta aatgtaaata ataacgattc ttatttacat acagagaccg ctcgcggcca
     3061 catcgtcacc aaataagcac gaacctcaga gcagacaatt atcatgctag ccaccgccat
     3121 catcgtgttt cgcgaagtcc tcgaagccgc gctcgttatc ggcatcgtgc tcgccgccac
     3181 gcagggtctg ctcaggcgcg ggacctgggt gagcgccggt gtcgtcgcgg gagtcgtcgg
     3241 ggcagggttg gtggcgttat tcgcggacgt catatcgtca gcctttgccg gtatcggcca
     3301 ggaagttttc aatgccggcg tattgctctt tgcggtgatc atgctcggtt ggcataacgt
     3361 gtggatgaaa cgccacggcc gtgaaatggc gcaacacatg cgcgacgtgg gtcaggcggt
     3421 caggcttggc agccgctcca tgtacatgtt ggcggtggtg atcggtcttg ctgtgttgcg
     3481 cgagggctcg gaagtggtgc tgtttctgta cggcattgcc gtggcgcagc aggattcgcc
     3541 cgccctgatg ttgtccggcg gtttgctggg actgttagcg ggtgcgatgg tgggtttact
     3601 catgtatcgc ggcctgctgc gcatatcgag ccgccatctg ttcacggtga cgacatggat
     3661 gatcacgttg ctggcggccg gcatggccgc gcaggcggcg ggttttctga cgcaggcgga
     3721 tctgctgccg ccgctggtgc aatccatgtg ggacacgtcg ggcatcgtgg cgcagaacag
     3781 cgttttcggg cgcgtgttgc agacgctgat cggttatgcc gaccgtccca gcggcatgca
     3841 ggtcctgttt tacgccgcca cgctcgccgg tatcgccgtg ctcatgcgtg tttatggcgg
     3901 ggcgctgtca cgcaaacccg tcgctgttac tgccgaaatt taattcatcc ccgcatgatc
     3961 gcctccggcc cgcgcgggga tttttttcag gctgtggttg tgaacaattc tcattaacta
     4021 ctaattttgg agatcttgac gatgaggtcg tgtaataaaa gaaaactcgc cattggcgcg
     4081 ttcctggcgt taacaggcat gcccggtatc gcgatggcag tggagtatcc gatcggcacg
     4141 ccgcagcagc agcacggcat ggagatcgcg gcggtgtatc tgcagccaat cgagatggag
     4201 ccggaaggca tgatgcgcaa ggctgttgaa tcggacgtac atctcgaggc cgacatccat
     4261 gcgctgtcga gcaatccgaa cgggttcgac gagggggcgt gggttccgag cctgctgatc
     4321 aaatacgagc tgaccaaagc cggcagcgat tggaaggcat ccggcgactt catgccgatg
     4381 gtggccaacg acggaccgca ctacggcgat aacgtcaagc tcggcggtcc aggcaagtat
     4441 cacctcaagt acaccatcta tgccccaaat gccccggaga atccctacgg caagcacttc
     4501 gggcgtcaca ccgaccgcct gacgggtgtg cggccttggt tcaaaccctt tgaggtcgag
     4561 tatgacttca ccttcgcggg catcggcaaa aagggcggtt attaatgaag ccctactcgc
     4621 tttgctcgtt cgtcttgcca tatgacgaga agccctactc gctgcgctcg ttcatcctga
     4681 catatcgaat gcgcgcgata aaatcgctgg cgctgcttgc gatcgcggcg tacgccggtg
     4741 cggccgtagg tgccgagccc aggtccgcat cggaggagat caaggaactc aaggagcgcg
     4801 tgcataaact ggagcaggaa aaggcgcgcg aggagaaagc actctccggg gaacgcgtga
     4861 gtgaagagga gcccgagatc gtcacgcgtc tgaaggccgt ggagtttcag tcactcggca
     4921 tgcaaaagca ggcgcgcacg atagagtcgc tcgaaggcat taccgccggt gtttcgttca
     4981 cgacggtggc gcaacacgct ggtggcagcg ccaccaccga tggctcgagc gaaagccagt
     5041 ggaactaccg cgccgatgtt tccg
//
LOCUS       DBNGZE010000223         6658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_31039, whole genome shotgun sequence.
ACCESSION   DBNGZE010000223 DBNGZE010000000
VERSION     DBNGZE010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_31039"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..686)
                     /locus_tag="AC16FV_05890"
     CDS             complement(123..686)
                     /locus_tag="AC16FV_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR2849623.1"
                     /translation="MKSPDSLANHFLIAMPSLADANFARSVTLICEHSEEGCMGIVIN
                     RLTDLHLGDIFDQLGIASDKAGHAETAVYLGGPVQNNRGFVLHEPLGDWESTLSVTDK
                     FGVSTSRDILEAIAENRGPEKFIVALGYAGWGAGQLEREITENSWLSGPANRDIIFNL
                     PVEQRWKAAASLVGVDLATLSSEAGHA"
     gene            complement(744..1634)
                     /locus_tag="AC16FV_05895"
     CDS             complement(744..1634)
                     /locus_tag="AC16FV_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR2849624.1"
                     /translation="MTAQTISGLSQTDRFGVSIFGSFLVHMVVILGVTFTLPKLRDLP
                     GLPSLEITLVQTASDKRPKDAEFLAQANQEGGGDSDAAEIAKNPLPVREISPVNRDFP
                     TFQSTPQKRVKSEREMITLLSQDASRKIKAQESRPDKKELQMQPPSLGLMTPTEILEE
                     RARLNAEISRTWQEFQKRPRHKFLNARTQEYKYAAYMDAWRAKVERIGNLNYPDEAKR
                     HQLTGNLLLDVALNADGSINEISVRRSSGQKILDDAAVRIVELSAPFAPFPPEIRADL
                     DVLHITRTWKFNESVLSSEN"
     gene            complement(1682..2650)
                     /gene="gshB"
                     /locus_tag="AC16FV_05900"
     CDS             complement(1682..2650)
                     /gene="gshB"
                     /locus_tag="AC16FV_05900"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR2849625.1"
                     /translation="MIKLGVVMDPIGSIKAYKDSTLAMLLEAQSRGWTIRYMEQGDLF
                     LRDGKAFAQQRALRVFDNTQRWFEWNEETTGPLSGLDVILMRKDPPFDMEYVYTTYLL
                     ERAENEGMLVVNQPRSLRDANEKLFIAWFPQCTPPTLVTRAAGRIRDFLAEQGDIILK
                     PLGGMGGESVFRLRRDDPNTSVVIETLTAHESRYAMAQRFIPEITQGDKRILLIDGEP
                     IPYALARVPAKGETRGNLAAGGTGVGVPLSERDRWICAQVGPTLREKGLLFVGLDVIG
                     DYLTEINVTSPTCIRELDRLYNLNISAKLMDAIAARLGKRRAQRQG"
     gene            complement(2726..4072)
                     /gene="gshA"
                     /locus_tag="AC16FV_05905"
     CDS             complement(2726..4072)
                     /gene="gshA"
                     /locus_tag="AC16FV_05905"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR2849626.1"
                     /translation="MGNESWAAVPHLTTALTGPLQEIEAHLLQNQAGIERWFRSQWLQ
                     TPAPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLNPAFQSLAVQAVQAALERICPSAGG
                     VLVIPENHTRNTYYFENLAALVEILTLAGLRVRVGSLLPDLHESRIIELPSGRRITLE
                     PLIREDNKVTVAGFNPCFALLNNDLSGGLPSILDNLDQPLVPPVSLGWWKRLKSRHFT
                     HYQQIVKEFSDIVRLDPWLINPVFRKCGKINFEKREGEECLANYIDEVLAEVRVKYRE
                     YGIDQEPFVIVKADAGTYGMAILTVKSADEVRSLNRKQRTKMSHLKEGQAVHDVMVQE
                     GVYTFETWGTKNAVAEPVVYMIDRYVIGGFYRVHTDRRYDENLNAPGMHFEPLAFAET
                     CAYPDPQQAPDANPNRFYAYGVIARLALLAAARESREAMETSDILKAKKATADERR"
     gene            4422..4808
                     /locus_tag="AC16FV_05910"
     CDS             4422..4808
                     /locus_tag="AC16FV_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2849627.1"
                     /translation="MSARVAHVSIKVMIIDDSNTIRRTAEALLKKAGYEVYTAADGFE
                     AMSMITDNHPDIIFVDIMMPRLDGYQTCQLIKNNKKFRDTPVIMLSSKDGLFDRARGR
                     IAGSEEHVNKPFTQEELIDVINKYVH"
     gene            4864..5226
                     /locus_tag="AC16FV_05915"
     CDS             4864..5226
                     /locus_tag="AC16FV_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2849628.1"
                     /translation="MAIKNVLIVDDSATDSHLLGEMLKKNGYTVLTATSGEEGIAKAK
                     KDKPDLILMDIVMPGMSGFEATRAISKDPETSAIPVIVCSTKGQETDKAWGLRQGAKD
                     YIVKPVVEKVLMEKIRAL"
     gene            5330..5872
                     /locus_tag="AC16FV_05920"
     CDS             5330..5872
                     /locus_tag="AC16FV_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR2849629.1"
                     /translation="MAQTRQDPFELLRKMQQESLENAPGLPQEVEATKLWSGVGFRLG
                     DIMLVTPLDHVLEILPPPPMTLVPGVKSWLKGVANVRGNLITIIDLPEYYGKPPVFMD
                     DKARMLIMNVQGLNAGLLVNEVLGLRHFDEELERQDLSGLDDPVLVQLNGAFLRDNVL
                     WGVFDMKSLAESPTFRHVAA"
     gene            5992..>6658
                     /locus_tag="AC16FV_05925"
     CDS             5992..>6658
                     /locus_tag="AC16FV_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025055.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HIR2849630.1"
                     /translation="MDQPSEDIIDLSDGGSSAAAADQVVEVGKPASALLSGGVMNMFG
                     SLPFLLGGMVIAMIAMIWLLVVNAQRSERESKYIEQSSQLLMLSQRLAKDAREAVLGQ
                     PTAFKTLKDSRDRFDAIVTSLRNGNPDQGVAPSPPEVATGPLQEIVKLWSPKPNEGLR
                     AQVDQILSLEKSLFNMHDSVMAINQMSPMLLTVTDEIVELGAKAGLKQDQLYLASRQG
                     MLSQ"
BASE COUNT         1325 a         1836 c         2101 g         1396 t
ORIGIN      
        1 gtttttggcg gagccgcgca gcgtggtcac gggttcggcc tggcgcgagt ggctgctgcc
       61 gaccgccatg ccgatatatt tttccccgta gtcgaaggcc aggtaagtgc gaacctggtc
      121 gttcaagcgt ggcccgcctc gctggagagt gtggcgaggt cgacgcccac gaggctcgcc
      181 gcggccttcc agcgctgttc cacaggcagg ttgaaaatga tgtcccggtt cgcggggccg
      241 gacagccagg agttttcggt aatctcgcgt tccagttgtc ccgcgcccca gccggcgtag
      301 ccgagggcca cgatgaattt ttccggaccg cgattttccg caatggcttc gagtatgtcg
      361 cgcgaagtgg aaacgccgaa tttgtcggtg accgaaagcg tggattccca gtcgccgagc
      421 ggctcatgca gcacgaagcc gcggttgttc tgcaccggac cgccgaggta aaccgccgtt
      481 tcagcgtggc cggccttgtc cgaggcgatg cccagctggt cgaaaatgtc gccgagatga
      541 aggtcggtca ggcggttgat cacgatgccc atgcagcctt cctcggaatg ttcgcagatg
      601 agcgtcacgc tgcgcgcgaa attcgcgtct gccaggctcg gcatggcgat cagaaaatgg
      661 ttggcgagcg aatcagggct tttcatgacg aacagtatcc ggcacccgga ggccagactc
      721 aagcaagcac gcgatccggc gcctcaattc tcgctggata gtaccgattc gttgaatttc
      781 caggtgcgcg tgatgtgcag cacatccagg tccgcgcgaa tctcgggcgg aaacggcgcg
      841 aaaggcgcgg acagttcgac gatgcggacg gcggcatcgt ccaggatttt ctgaccggaa
      901 gagcggcgca cggaaatttc gttgatcgaa ccatcggcat ttagcgccac gtcgagcagc
      961 aggtttcccg tgagctgatg ccgcttcgcc tcatcggggt aattgaggtt gccgatgcgc
     1021 tcgaccttgg cgcgccaggc gtccatgtag gcggcgtatt tgtattcctg cgtgcgggcg
     1081 ttgaggaact tgtgccgcgg gcgcttctgg aattcctgcc aagtgcggct gatctcggcg
     1141 ttgagacgcg cacgttcctc cagaatttcc gtcggcgtca tgagacccag actcggcggc
     1201 tgcatttgca attccttttt gtcaggccgg ctttcctgtg ccttgatttt acgggacgcg
     1261 tcctgcgaca gcagtgtgat catctcgcgc tccgacttga cgcgcttctg gggcgtggac
     1321 tggaaggtgg ggaagtcgcg gttgacggga ctgatctcgc gcaccggcaa cgggttcttg
     1381 gcgatctcgg cggcatcgct gtcgccaccg ccttcctgat tggcttgcgc gaggaattcc
     1441 gcatctttgg ggcgcttgtc gctcgcggtc tgtaccagtg tgatttcgag cgaaggcagc
     1501 cccggcaggt cgcgcagctt gggcagcgtg aaggtcacgc ccaggatcac gaccatatgc
     1561 accaggaacg agccgaagat gctcaccccg aagcggtcgg tttgcgaaag gccggagatc
     1621 gtttgggccg tcatgctcga aataaaaaca ccaagtccga attcgataca aaaagtatat
     1681 cttaaccctg ccgctgtgcg cgccgtttcc cgaggcgggc ggcgatggcg tccatgagtt
     1741 tggcgctgat gttcaggttg tacagccggt cgagttcgcg gatgcaggtc gggctcgtga
     1801 cgttgatttc cgtgaggtag tcgccgatca cgtccagccc cacgaacaac aatccctttt
     1861 cccgcagcgt ggggccgacc tgcgcacaga tccagcggtc acgctcggac aacggcacgc
     1921 ccaccccggt gccgccggcc gcgagattgc cgcgggtctc gcccttggcc gggacacgtg
     1981 cgagcgcata gggaatgggc tcgccatcga tcagcaggat gcgcttgtca ccctgggtga
     2041 tttccggaat gaagcgttgc gccatggcgt agcgggattc atgcgcggtc agggtttcga
     2101 tcacgacgct ggtgttcgga tcgtcgcgcc gcagacgaaa caccgactcc ccacccatgc
     2161 cacccagcgg tttcagaatg atatcgccct gctcggcgag gaaatcgcgg atacggccgg
     2221 cggcacgcgt caccagcgtg ggcggtgtgc actgcggaaa ccaggcgatg aaaagctttt
     2281 cgttggcgtc gcgcaggctg cgcggttggt tcaccaccaa catgccctcg ttctcggcac
     2341 gttccaggag ataggtcgta tagacatact ccatgtcgaa cggcgggtcc ttgcgcatca
     2401 ggatgacatc gaggccggac aggggtcccg tggtttcctc gttccattcg aaccagcgct
     2461 gggtgttgtc gaatacccgc agcgctcgct gctgtgcgaa ggcttttcca tcgcgcagaa
     2521 acaggtcacc ctgttccatg tagcggatcg tccagccgcg tgactgcgcc tcgagcagca
     2581 tggcgagtgt gctgtccttg taagccttga tggagccgat ggggtccatc accacgccga
     2641 gtttgatcat tgttatttat gatttatgga accgcaaaaa aacgcagaaa aagcaaaaaa
     2701 agtcctggat agattcgatc tgcatttatc tgcgttcatc tgcggttgct tttttcgctt
     2761 tgagaatgtc ggatgtctcc atagcttcac gggattcgcg cgcggcggcg agcagggcga
     2821 ggcgcgcgat gacgccgtag gcgtagaagc ggttgggatt ggcgtccggc gcttgttgcg
     2881 ggtcggggta ggcgcaggtt tcggcgaagg cgagcggctc gaagtgcatg ccgggggcat
     2941 tcaggttttc atcgtaccgg cgatcagtgt gcacgcggta gaaaccaccg atcacataac
     3001 gatcgatcat gtaaaccacc ggttcggcca cggcattttt tgtgccccag gtttcgaagg
     3061 tgtacactcc ttcctgcacc atgacgtcgt gcacggcttg gccttcttta aggtgtgaca
     3121 ttttggttcg ttgcttgcga ttgagtgaac gcacttcgtc ggcgctcttt accgtcagga
     3181 tggccatgcc gtaggtgccg gcgtcggcct tgacgatgac gaagggctcc tggtcgatgc
     3241 catattcgcg gtatttgacg cgcacctcgg ccagcacctc gtcgatataa ttggccagac
     3301 attcctcgcc ctcgcgtttt tcaaaattga tcttgccgca tttgcgaaag accggattga
     3361 tcagccatgg atcgaggcgc acgatatccg aaaattcctt gacgatctgc tgatagtggg
     3421 tgaagtgcct ggacttgaga cgcttccacc agccaagcga tacgggtggc accagcggct
     3481 ggtcgagatt gtccaggatc gacggcagtc cgcccgacag atcgttgttg agcagcgcga
     3541 agcagggatt gaaaccggcc accgtgactt tattgtcctc acggatcaac ggttcgagcg
     3601 tgatgcgccg gcccgagggc agttcgatga ttctggattc atgcaggtcg ggcagcagcg
     3661 accctacgcg cacgcgcagg ccggccagag tcaggatttc aacgagggcg gcgagatttt
     3721 caaagtaata ggtattgcgc gtgtgatttt ccggaataac cagcacgccg ccggcgctgg
     3781 ggcagatgcg ttccagcgct gcctgcaccg cctgcaccgc cagcgactgg aaggccggat
     3841 tgagattatt gaaaccgccc gggaacaggt tggtgtccac cggcgccagc ttgaatccgg
     3901 cgttgcgcag gtccacggaa caatagaaag gagcgggggt ctgcagccat tgggagcgga
     3961 accagcgttc gatgccggcc tggttctgca gcaggtgggc ctcgatttcc tgcagtggtc
     4021 cggtcagggc cgtggtcaga tgcgggacgg ccgcccaaga ttcgttaccc atggttccct
     4081 ggcatgcgtt ttatatgaat aaccttgact ccatggtatc cgattggttc tgcccaaggt
     4141 atgccagggg cttattggca tgaatcctgc tgacattcgg ctgtttttgg cgcttatggc
     4201 atgggccggg ctggtgaaga ccagtccggc gaagggattt cgagcactcg ctgaagcttg
     4261 gactatattg tgtagggaac ggccagcggg cggcacgtgc atggattttg ggtggaattt
     4321 atgctaccaa atcattgttg tagcaaaata actgcacaaa tcacatcaca caccgattta
     4381 tttccgcgcg cgcgggtcgt acacagcgtc cagcatgcag gatgtcggcg agggtagcgc
     4441 acgtgagcat caaagtgatg attattgacg acagtaatac gataagacgc acggccgagg
     4501 cgctgctgaa aaaggctggt tacgaggtgt acaccgccgc cgacggattc gaggccatgt
     4561 cgatgatcac ggataaccat ccggacatca ttttcgtgga catcatgatg ccgcgcctgg
     4621 acggatacca gacttgccag ctgatcaaga acaacaagaa gttccgggac acgccggtca
     4681 tcatgctgtc gagcaaggac gggcttttcg accgcgcccg cggacgcatc gccggctcgg
     4741 aagaacatgt gaacaagcct tttacgcagg aagagctgat cgacgtcatc aacaaatacg
     4801 tgcactgatc ggggcggatc ggcaaaggcg gaatttttta aaacgcagtg aatggagcgg
     4861 tctatggcca taaaaaatgt attgatcgtg gatgattcgg cgacggattc tcaccttctc
     4921 ggtgagatgc tcaagaaaaa cggctacacc gttctgaccg cgaccagcgg tgaggaaggc
     4981 atcgccaagg cgaagaagga caagcccgat ctgattctta tggacatcgt gatgccgggc
     5041 atgagcgggt tcgaggccac gcgcgccatc agcaaagacc cggaaacctc ggccattccg
     5101 gtcattgtct gttcgaccaa ggggcaggaa accgacaagg cctggggctt gcgccagggc
     5161 gcgaaggatt acatcgtcaa gccggtggtg gaaaaggttt tgatggagaa gatcagggcg
     5221 ctatagaaac gcagatgaac gcagataaaa aattgctgtt tcagtcctat ttaatccgcg
     5281 tttatccgcg catttatctg cgtccatctg tggacacagt tttttgacca tggcccagac
     5341 ccgacaggac ccgttcgagt tgctgcgcaa gatgcagcag gagagcttgg agaatgcccc
     5401 cgggctcccg caggaagtcg aggccaccaa gctgtggtcc ggcgtaggat tccggttggg
     5461 cgacatcatg ctggtgacgc cgctggatca cgtgctcgaa atcctgccgc cgccgccgat
     5521 gacgctggtg ccgggcgtga agtcgtggct caagggcgtg gccaacgtgc gcggcaacct
     5581 gatcacgatc atcgacctgc cggagtatta cggcaagccg ccggtgttca tggatgacaa
     5641 ggcgcgcatg ctcatcatga atgtgcaggg cctgaatgcg ggattattgg tgaacgaggt
     5701 gctgggactg cggcacttcg acgaggagct ggagcgccag gatctgtccg ggctggacga
     5761 tccggtactg gttcagctga acggcgcgtt tttgcgcgac aacgtactgt ggggtgtctt
     5821 cgacatgaag tcgttagcgg aaagccccac gttccggcat gtcgcggctt gagttgtgcg
     5881 gggattttgc gggacatctt tggcttcagg gcgcatgaaa tttttttgac agatctcagt
     5941 tcgagatgag gtgacagcat ggctagaaaa aattcttccc aggacggtct gatggaccaa
     6001 ccctccgagg atattatcga tctctcggat ggcggttcct ccgcggcggc tgcggaccag
     6061 gttgtcgagg tcggcaagcc ggcgtcggcc ctgctgtccg gcggcgtgat gaacatgttc
     6121 ggcagcctgc ccttcctgtt gggcggcatg gtgatcgcga tgattgccat gatctggctg
     6181 ctggtggtca acgcccagcg gtccgagcgc gaatccaaat acatcgagca gtcctcgcaa
     6241 ctgctgatgt tgtcgcagcg cctggcgaag gacgcccgcg aggcggtgct gggccagccg
     6301 accgcattca agacgctcaa ggactcgcgc gacaggttcg acgccatcgt cacctccctg
     6361 cgcaacggca atcccgacca gggtgtggca ccgagcccgc cggaggtcgc caccgggccg
     6421 ttgcaggaga tcgtcaagct ctggtcgccc aagccgaacg aaggcttgcg cgcgcaggtg
     6481 gaccagattc tgtctctgga aaaatccctg ttcaacatgc acgattcggt gatggccatc
     6541 aaccagatga gcccgatgct gctgacggtt accgacgaga tcgtggaact cggcgccaag
     6601 gctgggctga aacaggacca gctttacctc gccagccgcc agggcatgtt gtcgcaac
//
LOCUS       DBNGZE010000224         2025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1350633, whole genome shotgun sequence.
ACCESSION   DBNGZE010000224 DBNGZE010000000
VERSION     DBNGZE010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1350633"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16FV_05930"
     CDS             complement(<1..770)
                     /locus_tag="AC16FV_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR2849631.1"
                     /translation="MNSAIIFGLLIALMLTGMPISIALGLTVLSFLFVMTTVPIEAVA
                     LKLFTGIESFEIMAIPFFILAGNFLTHGGVARRMIRFATSMIGHWYGGMGLAGVMACA
                     LFAAVSGSSPATVVAIGSIMLPAMVQQGYPKRFGAGVIATSGALGILIPPSIVMVLVA
                     VATGGSVAFDPEGKRVLSASVAQLFMAGVVPGLILATMLGMTTFYRAWKNNYPRMQKA
                     SWLEAVVAFRDSVWGLLLILIVLGGIYTGAFTPTEAAAV"
     gene            complement(767..1336)
                     /locus_tag="AC16FV_05935"
     CDS             complement(767..1336)
                     /locus_tag="AC16FV_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR2849632.1"
                     /translation="MFLRILDRLEETLIASLIAAATLLIFVAVVQRYMVGVPFLYPLV
                     YHINLTWAQELCIYMFVWMAKFGAAYGVRTGIHVGVDVVVNMLDPGPRKKVVLFGLMC
                     GALFTFVVGTMGAKFVIELYPTDQVSPDMEMPRWIIFLCVPLGSYLMCFRFLQVAWAF
                     WRTGELPHHDPGHVEGVVESPAAARDLAR"
     gene            complement(1504..>2025)
                     /locus_tag="AC16FV_05940"
     CDS             complement(1504..>2025)
                     /locus_tag="AC16FV_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR2849633.1"
                     /translation="EDYKGLKFRIQSSKVLEAQFRLLGATPQVMAFGEVYQALQTGVV
                     DGQENTPSNIYTQKFYEVQKYTALTNHGYIGYVVVVNKKFWDDLPADVRGQLEKAMNE
                     ATAYANKISESENAEALDEIKKSGKTEIIIPTGAEMAAMQKAMEPLYQDMASRVGKQL
                     IDDVRKTAQGVTN"
BASE COUNT          429 a          629 c          606 g          361 t
ORIGIN      
        1 accgcggcgg cttcggtcgg ggtgaaggca ccggtataga tgccgccgag cacgatcaag
       61 atcagcagca ggccccacac cgaatcgcgg aaggcgacta ccgcctctag ccagcttgcc
      121 ttctgcatgc gtggatagtt gttcttccag gcgcggtaga acgtggtcat gccgagcatg
      181 gtggccagga tcagacccgg gacgacaccc gccatgaaca gttgcgccac cgaggccgac
      241 agtacgcgct tgccttcggg atcgaaggcc acactgccgc cggtcgccac cgcgaccagc
      301 accatcacga ttgagggcgg aatcaggatg ccgagcgcgc ccgatgtcgc gatcacgccg
      361 gcgccgaaac gttttggata accctgctgt accatcgccg gaagcatgat cgagccgatt
      421 gcaacgacgg tcgcgggcga ggaccccgac acggctgcaa acagtgcgca ggccatcacg
      481 ccagcgagac ccatgccgcc ataccagtgg ccgatcatgg aggtggcgaa ccgaatcatg
      541 cggcgagcga cgccgccatg ggtcaagaaa tttccggcaa gaataaagaa tgggatcgcc
      601 atgatctcga agctctcaat gccggtgaag agcttgagcg ctaccgcctc gatcggcacc
      661 gtggtcatca caaacaggaa gctgagcacg gtcaggccga gcgcaatcga aatcggcatg
      721 ccggtgagca tcagcgcgat cagcaggccg aagatgatgg cgctgttcat cgggccagat
      781 ccctggctgc agcggggctc tcgaccacgc cctcgacatg gccgggatca tggtgcggca
      841 gctcgccggt gcgccagaag gcccatgcga cctggaggaa ccggaaacac atgagataag
      901 agccgagcgg cacgcagagg aagatgatcc agcgcggcat ctccatgtcg ggcgaaacct
      961 ggtcggtggg gtaaagctcg atcacgaact tcgcgcccat ggtgccgacc acgaaagtga
     1021 atagcgcgcc gcacatcagg ccgaacagca cgaccttctt gcgcggtcca gggtccagca
     1081 tgttgaccac aacgtcgacg ccgacatgga tgccggtgcg cacgccatag gcggcgccga
     1141 acttcgccat ccacacgaac atgtagatgc acagttcctg cgcccaggtc aggttgatgt
     1201 ggtagacaag cggatagaga aacgggacgc ccaccatgta gcgctgcacg acggccacga
     1261 aaatgagcag cgtggcagcc gcaatcagac tcgcaatgag agtttcttcg agacgatcga
     1321 gaatgcgaag gaacacttac gcctcgtgcg cttggggact atgacgtgcc ccagcaggcg
     1381 gcaattcccg gattaacgac agccgctgcc cccacggccc tcgccggaca tcgatcaaca
     1441 cgcgggaccc cctacccctt gaagtaattg agccccggtg cagaggccgg ggctcgccgt
     1501 tttttagttg gttacgccct gcgccgtctt gcgaacatcg tcgatcaact gcttgccgac
     1561 gcggctggcc atgtcctgat agagcggttc catggccttc tgcatagcag ccatttcggc
     1621 accggtcggg ataatgatct ccgtcttgcc cgacttcttg atctcatcca gcgcctccgc
     1681 attctccgac tcggagatct tgttggcgta ggcggtcgct tcattcatcg ccttctcgag
     1741 ctggccacgg acatcggccg gcaggtcgtc ccagaacttt ttgttcacga cgaccacgta
     1801 gccgatatag ccgtgattgg tcagcgccgt gtacttctgc acctcataga atttctgggt
     1861 gtagatattt gacggcgtgt tttcctggcc gtcgaccacg ccggtctgca gcgcctgata
     1921 gacttctccg aacgccatca cctgcggcgt tgcgccgagc aggcggaatt gcgcttccag
     1981 caccttcgat gattggatgc ggaatttcag acccttgtag tcctc
//
LOCUS       DBNGZE010000225         6213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1142660, whole genome shotgun sequence.
ACCESSION   DBNGZE010000225 DBNGZE010000000
VERSION     DBNGZE010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1142660"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /gene="yidC"
                     /locus_tag="AC16FV_05945"
     CDS             <1..1495
                     /gene="yidC"
                     /locus_tag="AC16FV_05945"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR2849634.1"
                     /translation="APEPPAAETATAGPKPAEALASGMRIQVRTDMIDAVIDSQGGDL
                     RELKLRHYPVTLDKPEEAFPLLRDAGPELFIAQSGLIGLDGQYPTHKTPFTAEAGHYT
                     LAGSQNELRVPLSWRTPGGVQYTKTYIFHRGSYVVDVEFAVKNDTRREWSGYLYGQFQ
                     RMHTATTGIFNVAPSYTGGAIYTPENKYEKVPLDKMAASPLKREATGGWVAILQHYFV
                     GSWMPAKDERNEFYTDVLDGTRHVLGFKNLNPTKVAPGANGVIRTSLYAGPKEQKILK
                     TLPAEGMNLTVDYGFLTVISAPLFWLLSWFHRLLGNWGWAIILLTVLIKAVFYPLSAA
                     SYKSMAHMRKMQPKMQALKERFGDDRQKLNQAMMELYKTEKINPLGGCLPILIQIPVF
                     LALYWVLLESVEMRQAPWILWIKDLSSQDPYYILPIIMGVSMFVQQKLNPQPLDPIQQ
                     KVFMVKPFIFTAMFLIFTAGLVLYSTVNNILSIAQQWRITRVVEGKK"
     gene            1514..2860
                     /gene="mnmE"
                     /locus_tag="AC16FV_05950"
     CDS             1514..2860
                     /gene="mnmE"
                     /locus_tag="AC16FV_05950"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR2849635.1"
                     /translation="MIATATIAAIATPPGRGGIGIVRISGPATRNIAEGLLGKLPNPR
                     HASFANFMDAASQPIDQGLAIFFPAPNSFTGEDVLELHGHGGSVVLDMLLKRALELGA
                     RLARPGEFSERAFLNGKLDLAQAEAVADLIEAGSETAARSALRSLEGEFSQRVHVLVE
                     GLTRLRLYVEAAIDFPEEEIDFLADERAMQELDMLARDIEKLLASTQQGVLLHDGMTV
                     VLAGPPNAGKSSLLNALAARDTAIVSAIPGTTRDVLRERIHLDGMPLHIVDTAGLRDS
                     RDEIESEGIRRAREQMERADRVLLVLDDSSHDALPAEVLHHVPAKLPRSVIRNKIDIT
                     GRAAGVTKTPNETEIALSAKTGEGLNDLRQHLKECMGFQPAGEGAFMARRRHLDAIRR
                     AQACLVQGKARLQESRAGELLAEELRLAQEALSEITGEFTPEDLLGKIFSSFCVGK"
     gene            complement(2983..4125)
                     /locus_tag="AC16FV_05955"
     CDS             complement(2983..4125)
                     /locus_tag="AC16FV_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR2849636.1"
                     /translation="MELPTQAYIKPATKDSADKIVALPILQRGGLRILSWIAFGLNTA
                     TRLQPPVTRALARFRAPTWASKTTIARVLFLGLGLFTLLPIVSAGIALLMGMFFALTI
                     GNPYLLTTRSYAPILLQVSVVGLGAAMNLMTIAVVGMHGALYTAVGISMTLVLGLALG
                     RLIRTNYTTSLLLSVGTAICGGSAIAAVASTMRTKDHEVSLSLTIVFFLNALALFLFP
                     WIGHRAGLDESQFGLWSALAIHDTSSVVGAASQYGTKALDVATTVKLARALWIVPVIF
                     VVSALWMRKEGASAQSKVKRPWFILGFVLLAALVTWVPALIPAGHVISEISQRILVLT
                     LFLIGSGMTCASLKSIELRPLVHGLTLWIIVASVSFLAISIGWVKI"
     gene            4454..4630
                     /locus_tag="AC16FV_05960"
     CDS             4454..4630
                     /locus_tag="AC16FV_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849637.1"
                     /translation="MNAESLMYKIHLVVFLSVLNIFGTMVMAAQTHGPDSAAVHTPAV
                     TAESTAHIYQLSPY"
     gene            5060..>6213
                     /locus_tag="AC16FV_05965"
     CDS             5060..>6213
                     /locus_tag="AC16FV_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR2849638.1"
                     /translation="MMNGLSIKMRLYINMGLALMVMLILTGVDLYSVRQGSSALESVY
                     ERWVVPTSDLTAVDADLKDLRFRMAAYLVDQMPAVGNINQLGEARMQVPERWKKYKAL
                     TEQDKLNDEARELIGTIDNQIAILGPFLDKLEAAYRANRKAQVTSMLENEWPQVHAGL
                     LKPISSLLKTQETAVKSTYDHYAANGWRLISFVLIVLVAVMVVMIYSGLRNVQAIGKP
                     LEQAAALATRIASGDLTGKVEVRNHDEVGRLLMALKQMNDNLRRLVDGIRSSADSFAH
                     GSQEMSSAAEQLSSSAQEQASSLEETAASMEEMTSTVKQNADNSLRADKVASEAREAA
                     NEGVVMASSVQRSMEAINASSTKIADIIGVIDEIAFQTNLLALNAAVEAAR"
BASE COUNT         1369 a         1806 c         1805 g         1233 t
ORIGIN      
        1 ggcgccggag cccccagccg ccgaaacggc aaccgccggc cccaaacctg ccgaggcgct
       61 ggcgagcggt atgcgtatcc aggttcgcac cgacatgatc gacgccgtga tcgacagcca
      121 gggtggcgac ctgcgtgaac ttaagctgcg ccattacccg gtgaccctgg acaagccgga
      181 ggaggccttt ccactgctgc gcgatgccgg gccggaactg tttattgccc agtcgggatt
      241 gatcggtttg gacggtcagt atccaaccca caagacaccg ttcactgccg aggccggtca
      301 ttacacgctg gccggcagcc agaatgagct gcgcgtgcca ctgagctggc gcacccccgg
      361 cggtgtgcaa tacaccaaga cttacatctt ccatcgcggc agctacgtcg tggatgtaga
      421 gtttgcggtg aagaatgaca cgcgccgcga gtggagcggt tacttgtacg gccagttcca
      481 gcgcatgcac acggctacca ccggcatttt caacgtggcg ccgagctaca ccggtggcgc
      541 gatttatacc cccgaaaaca aatacgaaaa agtgccgctc gacaaaatgg ccgcgagccc
      601 gctcaagcgc gaagccacgg gtggatgggt ggcgatactg cagcactatt tcgtcggctc
      661 gtggatgccg gcaaaggacg agcgcaatga gttctatacc gacgtactgg atggaacgcg
      721 ccatgtgctc ggcttcaaga acttgaaccc aaccaaggtt gcgcccggcg cgaatggcgt
      781 catccgcacc tcgctgtacg ccggccccaa ggaacagaag attctcaaga ccttgccggc
      841 cgagggaatg aaccttacgg tggattacgg attcctcacc gtcatttccg cgccgctgtt
      901 ctggttgctg tcttggttcc accgcctgct gggcaactgg ggctgggcga tcatactgct
      961 cacggtcctg atcaaggccg tgttctatcc gctgtcggcg gcgagttaca agtccatggc
     1021 gcacatgcgc aagatgcagc ccaagatgca ggcgctcaag gaacgcttcg gcgacgatcg
     1081 ccagaagctc aaccaggcga tgatggagct gtacaagacc gagaagatca atccgctcgg
     1141 cggttgtctg ccgatcctga tccagattcc ggtgttcctc gctctttact gggtgctgct
     1201 cgagagcgtc gagatgcgcc aggcgccgtg gatcctgtgg atcaaggatc tgtcatcaca
     1261 ggatccgtat tacatcctgc cgatcatcat gggtgtttcg atgttcgtac agcagaagct
     1321 caatccgcag ccgctcgacc cgatccagca gaaggtgttc atggtcaagc cgttcatatt
     1381 caccgccatg ttcctgatct tcactgcggg cctggtgctg tactcgacgg tgaacaacat
     1441 cctgtccatc gcgcagcagt ggcgcatcac gcgcgtcgtc gagggcaaga agtaacgctg
     1501 tatcacgctg gccgtgatcg ccaccgctac cattgccgcc atcgccacgc cacccgggcg
     1561 cggcggcatc ggcatcgtgc gcatctccgg tccggctaca cgaaacatcg ccgagggttt
     1621 gctcggtaaa ctccccaatc cgcgccacgc gagtttcgct aattttatgg atgcggccag
     1681 ccagcccatc gaccagggtc tggcgatatt ttttcccgcg cccaactcct ttaccggtga
     1741 agacgtgctc gaactgcatg gccacggtgg atccgtggtg ctcgacatgc tgctcaagcg
     1801 cgcactcgaa ctcggcgcgc gtcttgcaag accgggggaa ttttccgagc gcgcgttcct
     1861 gaacggcaag ctcgatctgg cccaagccga agcggtggcc gatctcatcg aggccggctc
     1921 ggagacggcg gcgcgctcgg cgctgcgctc gctcgagggc gagttttcgc aacgcgtgca
     1981 cgtgctggtt gaaggcctca cgcgcctgcg cctgtacgtc gaggccgcga tcgattttcc
     2041 ggaagaggaa atcgatttcc tggcggacga gcgcgcgatg caggagctcg atatgctcgc
     2101 gcgggacatc gaaaaacttc tcgcttccac gcagcagggc gtgttgctgc acgacggcat
     2161 gaccgtagtt ctggccggcc cgccgaatgc cggcaagtcg agtctgctga atgcgctggc
     2221 cgcgcgcgac accgccatcg tcagcgccat tcctggcacc acgcgcgacg tactgcgcga
     2281 gcgcatccac ctcgacggca tgccgctgca tatcgtcgac accgccggcc tgcgcgactc
     2341 gcgcgacgag atcgaatccg agggcatccg ccgcgcgcgc gaacagatgg aacgcgccga
     2401 ccgcgtgctg ctggtgctgg atgattcatc ccatgacgcg ctcccggccg aagttctaca
     2461 tcatgtcccg gcaaaacttc cacgtagcgt cattcgcaac aagatagata tcactggtcg
     2521 cgcagccggc gtcacgaaaa caccaaatga aacggaaatc gccctgtcgg ccaaaaccgg
     2581 ggaagggttg aatgacctgc gtcagcacct gaaagaatgc atgggcttcc agcccgcggg
     2641 cgaaggcgcg ttcatggcgc gccggcgcca tctcgacgcc atccgccgcg cgcaggcctg
     2701 tctggttcag ggcaaggcgc ggctccagga atcgcgcgcc ggggagttgc tggcggaaga
     2761 actgcgcttg gcgcaggagg ctctgtccga aatcaccggc gagttcacgc cggaggattt
     2821 gctggggaaa attttttcca gcttttgtgt cggaaagtga cgtaagcccg ctgacatctg
     2881 gcgatgtcca aatttagccg aagcacagcg cctttgtgct cctctctaaa aagaggcggc
     2941 cggcacccgg ttccaagccc tttccacaca cgtgcctttt atttatatct ttacccatcc
     3001 tattgaaatt gccagaaagc tcacgcttgc gacgatgatc cacaacgtaa gcccgtggac
     3061 caacggacga agttcgatgc tcttcagcga tgcgcaggtc atgccgctgc cgatcaggaa
     3121 caatgtaagc acaaggattc gctgcgagat ctcagaaatc acatgaccag ctggtattag
     3181 ggcaggcacc caagtgacga gagccgccag caagacaaat cccagaatga accaaggccg
     3241 cttcactttg gattgagcgc ttgctccttc ctttcgcatc cagagtgcgc tgaccacaaa
     3301 tatcaccgga acgatccaga gcgctcgtgc caatttcaca gttgtggcga catccagcgc
     3361 tttggtccca tattgcgacg ccgctccgac caccgagctt gtgtcgtgta tggccaatgc
     3421 gctccaaagg ccgaactggc tttcgtcgag gcccgccctg tgcccgatcc aggggaacag
     3481 aaacagcgcc aaagcgttta gaaaaaatac gatcgtcaga gacagcgata cctcatgatc
     3541 ttttgtccgc atggttgatg cgaccgcggc gatcgcactc cccccgcaaa tcgcggtgcc
     3601 tactgagagc agaagcgagg tcgtgtaatt agttcggatt agccttccca gcgcgaggcc
     3661 gagaaccagc gtcatcgata tgccgacagc ggtatagagc gcgccgtgca ttccgacgac
     3721 ggcaatggtc atgagattca tcgccgcgcc aagtccgacg acggatactt gcagtaatat
     3781 gggtgcgtag ctgcgagtcg tcagaaggta aggattgccg atggtgaggg cgaagaacat
     3841 acccatcaag agggcgatgc cggccgatac gatcggcagc agtgtaaata aaccaagccc
     3901 aagaaagaga acccgagcga tcgtggtttt agacgcccat gtcggagccc gaaagcgggc
     3961 aagcgcgcgg gttacgggcg gctgtaatcg agtcgcggta ttcaacccga aagcgatcca
     4021 ggaaaggatg cgcaagcccc cacgctgcag gataggcagc gcaacgatct tatccgctga
     4081 atcttttgtc gcaggcttta tgtaagcctg agttggtaat tccacttttt agagtccaag
     4141 cgtgacaccg gtctaaccga tgccatgtcg gaccattggg ttgaagacaa tccctgcccg
     4201 aagtagatgc aaggaatata ccaaggcaat cggtgttgcg agacccggcg agcgacggca
     4261 gggtgtgctg cttgagaatt ggctcccccc ttatgtttct tcggactcgg cacgacaagc
     4321 aaattttgga tatccccctg tcgaaatttg cattgggggg attatccagc tctttgaaaa
     4381 gacagcctta ttgagttggc acggagcttg ctgctcctta tctggcggtt taattactca
     4441 aaggagagta ttcatgaacg cagaatccct gatgtacaaa atccatttgg tggttttctt
     4501 gtccgtgtta aacatattcg gcaccatggt catggcggcc cagacacacg gtcctgatag
     4561 tgcggctgtt cataccccgg ctgtcacggc tgaatccaca gcccacattt accagcttag
     4621 cccctactaa ataatcggtt tccagtcgaa ttggccacgg acggccgcgg gcttgaagtc
     4681 ctgggtcgtg ggtggcgtct ccaatcctgt acctgcaccg caaagattat ctattgtcca
     4741 gcgcggaatg gcggtcgaga ttcgcgccga ctatgctgaa cattagcgat gtggcgatag
     4801 tgtaagacta tcctgtcgag cggcaattca atgttccaat cgcccagttt tcgccggtct
     4861 ccaaaatgcc aggtgttctt cagccttaaa tccaggatca acagcacctg atataactgg
     4921 catcaacttt gcattaagag aatcggtatg agcaacaatg caagatcggc ctggtataag
     4981 caggcccggg cttcccgtcg agtatgttgg ttttcttgac tacgcggcag gaaacccacg
     5041 tccggagcga tacgcaatta tgatgaacgg cctaagtatc aaaatgcggc tctacatcaa
     5101 catgggcttg gcgctcatgg tgatgttgat tctaaccggg gtggacctct acagcgtcag
     5161 acagggttcg agcgcgcttg aatctgtcta cgagcgctgg gtggtcccga catcggatct
     5221 cacggctgtt gacgccgatc tgaaggattt acgcttccgc atggcggcct atctggtgga
     5281 tcagatgccg gcggttggca acatcaatca gcttggcgag gcgcgcatgc aggtaccgga
     5341 gcgctggaag aaatacaaag cgctcacgga acaggacaaa ctcaacgacg aggcgcgcga
     5401 gctgatcgga acgatcgata atcaaattgc gattctgggg ccttttctgg acaaattgga
     5461 ggcggcctac cgcgcgaaca gaaaagcgca ggtaacctca atgctggaaa acgaatggcc
     5521 gcaagtccat gccggcctgc tgaagccgat ttccagcctg ctgaagaccc aagagaccgc
     5581 ggtcaagagt acctatgatc attatgcggc caacggctgg cggctgatct cgtttgtact
     5641 gatcgtactc gtggctgtta tggtggtaat gatctattcc ggattgcgta acgttcaggc
     5701 cattggcaaa ccgctcgagc aggcggctgc cctggccacg cgcatcgcct cgggcgatct
     5761 caccggcaaa gttgaagtac gcaaccacga cgaagtcggg cgattgttaa tggcgctgaa
     5821 gcaaatgaac gataacctgc gtcgtctggt tgacggcatt cgcagcagcg ccgactcgtt
     5881 tgcgcacggc tcgcaagaga tgagttccgc ggcagaacaa ttatcctcct ccgcccagga
     5941 gcaggccagt tcgctcgaag aaaccgccgc gtctatggaa gagatgacca gcacggtaaa
     6001 gcagaatgcc gacaactcgc tgcgcgcgga taaagtggcc tcagaggcgc gcgaggcggc
     6061 aaacgaggga gtggtgatgg cctcctcggt tcagcgctcg atggaggcga tcaacgcgag
     6121 cagcaccaag attgcggaca tcatcggcgt gatcgacgag atcgcgttcc agaccaacct
     6181 gctggcgctc aatgccgccg tcgaggcggc gcg
//
LOCUS       DBNGZE010000226         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_725809, whole genome shotgun sequence.
ACCESSION   DBNGZE010000226 DBNGZE010000000
VERSION     DBNGZE010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_725809"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16FV_05970"
     CDS             complement(<1..279)
                     /locus_tag="AC16FV_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2849639.1"
                     /translation="MKINPVILSGGSGTRLWPLSREHYPKQLLCLLGEQTLLQQTVSR
                     LDGMGNVAAPLLVCNEEHRFLIAEQLRQMEKAPADIILEPVGRNTAPAL"
     gene            complement(324..1337)
                     /locus_tag="AC16FV_05975"
     CDS             complement(324..1337)
                     /locus_tag="AC16FV_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HIR2849640.1"
                     /translation="MTNILITGVAGFIGSALALRLLERGDTVYGIDNMSNYYDVHLKE
                     ARLKRTIDHHKFRFEKLDIVNRKGMAELFHREKFDAVMHLAAQAGVRYSITNPDAYID
                     ANLVGFGNILEGCRHNGVKHLVFASSSSVYGANTRLPFSEHDNVDHPVSLYAATKKAK
                     ELMAHSYAHLYRLPCTGLRFFTVYGPWGRPDMALFKFTKGILEGTAIPVYNHGKMIRD
                     FTYIDDIVEGVVRAIDQPARPDPAWSGNAPDPATSYAPYHIYNIGNNQPVELMRYIEV
                     LEKSLGKKAKLEMLPMQDGDVPATSANVDSLSKDVGFRPQTSVELGISRFVEWYKSFY
                     GVA"
     gene            complement(1334..2305)
                     /locus_tag="AC16FV_05980"
     CDS             complement(1334..2305)
                     /locus_tag="AC16FV_05980"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR2849641.1"
                     /translation="MAENKPILGRKRILITGGAGFLGSHLSERLLRDGHDVLCVDNFF
                     TGTKDNIVHLLGNPHFEHLRHDVTFPLYVEVDEIYNLACPASPIHYQFDPVQTTKTSV
                     HGAINMLGLAKRVKAKIMQASTSEVYGDPAVHPQTEDYWGNVNPIGFRSCYDEGKRCA
                     ETLFFDYRRQHNLRIKVARIFNTYGPRMHPNDGRVVSNFIVQALKGEPITVYGDGKQT
                     RSFCYVDDLIEGFVRLMNTPDDFTGPVNLGNPGEFTIRELAEKIIDLTGSKSKIINKP
                     LPSDDPRQRQPDISLARSKLGWSPTTTLDQGLPKTIAYFDNLLKSFP"
     gene            complement(2438..3040)
                     /locus_tag="AC16FV_05985"
     CDS             complement(2438..3040)
                     /locus_tag="AC16FV_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR2849642.1"
                     /translation="MNTKADREEERVLELLDAVGKQSNVSQRHLANNMGIALGLANSY
                     LKRCIRKGFIKISEAPANRYLYYLTPKGFAEKSRLTAKYLSTSFAFYRRAGESCMDVF
                     HRCETRGWRKILLYGMSDLAEIAMLRAEETDVDIVGILDPHTERRRFAGVSVWHSLQQ
                     VEPYDGCVLTELNSPLLSYEQLLKEMEKERILIPGILRLE"
     gene            complement(3092..3616)
                     /gene="rfaH"
                     /locus_tag="AC16FV_05990"
     CDS             complement(3092..3616)
                     /gene="rfaH"
                     /locus_tag="AC16FV_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HIR2849643.1"
                     /translation="MNTATNDSAARAWYLVYCKPRQESVARENLARQGYETYLPFMRD
                     VRRRQGKRVALIAPMFPRYLFIHLNRQTDNWAPIRSTLGVVSIVRFGRAAARVPDELL
                     AMLRSREDTQGIQILPVEEYKPGSRVRITQGGFAGYEGIFQAATGRDRVTVLLDVLGR
                     KARATVDSASIEPA"
     gene            complement(3625..>3860)
                     /locus_tag="AC16FV_05995"
     CDS             complement(3625..>3860)
                     /locus_tag="AC16FV_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR2849644.1"
                     /translation="PVADVEEARHEYGIELHSWESLAKAKAIVAAVAHKPFLGMAIDE
                     YLKKLERGGCFIDVKSRFNTKAFTDAGMHVWRL"
BASE COUNT          840 a         1100 c         1061 g          859 t
ORIGIN      
        1 cagagccggc gccgtgttgc gccccaccgg ctccaggatg atgtccgccg gtgccttttc
       61 catctgtcgc aactgttccg cgatcaggaa acgatgctcc tcgttgcaca ccagaagcgg
      121 agccgcgacg tttcccatcc catccaggcg ggagaccgtc tgctgcaaca gtgtctgctc
      181 gcccagcaga cacaataatt gtttggggta gtgttcacgc gagagcggcc atagccgcgt
      241 tccggaacca ccggacagta tgactggatt gattttcatg aatgcgggat tctcgacgtc
      301 agaaacggga actcggttgc atctcaggcg acaccataaa atgacttata ccactccacg
      361 aagcggctga ttccaagctc gacggaagtt tgcggtcgaa aaccgacgtc cttgcttaaa
      421 ctgtcgacat tggctgaagt cgccggcaca tccccgtcct gcattggcag catttcgagc
      481 ttggccttct tgcccaggga cttttccagc acttcaatgt agcgcatcag ttcgaccggc
      541 tggttattgc caatattata gatatggtaa ggcgcgtaac tcgtggccgg atcgggcgca
      601 ttgccggacc aggccggatc gggccgtgcc ggctggtcta ttgcacgaac aaccccttca
      661 acaatgtcat caatataggt aaaatcgcgg atcatctttc cgtgattgta aaccggtatg
      721 gccgttcctt ccaatatacc cttggtgaat ttaaacagcg ccatgtccgg ccgcccccag
      781 ggaccataaa ccgtgaaaaa ccgcaaaccg gtgcacggca accggtacag atgcgcgtaa
      841 ctatgcgcca tcaactcctt ggcttttttc gtggccgcat acaaggaaac cggatgatcc
      901 acgttgtcat gttcggaaaa aggaagtctc gtattggcgc cataaaccga gcttgatgag
      961 gcaaatacga gatgtttcac gccattatgc cggcagcctt ccaggatatt gccgaagccg
     1021 acgagattgg catcgatata agcatcgggg ttggtaatcg aataccggac acctgcctgc
     1081 gccgccagat gcatcaccgc gtcgaatttt tccctgtgga acaattcggc cattcccttg
     1141 cggttgacga tatccagctt ttcgaaccgg aacttgtgat gatcaatcgt gcgcttgagg
     1201 cgcgcctcct tgagatgaac atcgtaataa ttgctcatat tatcgattcc atacaccgta
     1261 tcgccacgct ccagcagcct gagcgcgagc gcggaaccaa tgaaaccggc aactccagta
     1321 atgagaatat ttgtcacgga aaactcttca gcaaattgtc aaaataggca atagttttgg
     1381 ggaggccctg gtcgagggtg gtggtcggcg accacccgag tttcgaccgg gccagggaga
     1441 tgtccggctg acgctgccta ggatcgtcgc tgggcagcgg tttgttgatg attttcgatt
     1501 tcgagcccgt caaatcgatg atcttctcgg cgagctcgcg tatggtgaat tcacccggat
     1561 ttccaagatt gaccgggccg gtgaagtcat ccggggtgtt catcaatcga acaaatccct
     1621 cgatcaggtc atctacatag cagaatgagc gtgtttgttt gccgtcgccg taaaccgtga
     1681 tgggctcgcc cttgagcgcc tgcacgatga aattcgaaac gacgcggccg tcgttcgggt
     1741 gcatgcgcgg accgtaggtg ttgaatatgc gcgccacctt gatacgtagg ttgtgctggc
     1801 ggcggtagtc gaagaacagc gtttcggcgc aacgcttgcc ttcgtcgtag cacgagcgaa
     1861 acccgatcgg attcacgttt ccccagtaat cctcggtctg cgggtgtacc gccgggtctc
     1921 cgtaaacttc gctggtggag gcctgcatga tcttggcctt gacccgtttc gccagcccca
     1981 gcatattgat cgcgccgtgc acgctggtct tggtcgtctg caccggatcg aactggtaat
     2041 gaatcgggga ggccggacag gccaagttgt agatttcatc cacctcgacg tagagcggga
     2101 acgtcacgtc gtggcgcaaa tgctcgaaat gcgggttccc caagagatgc acgatgttgt
     2161 ccttggtgcc cgtgaagaaa ttgtccacgc acagcacgtc gtggccgtcc cggagcaggc
     2221 gctcgctcag gtgggagccg agaaaaccgg ccccgccggt gatcaggatg cgtttgcgtc
     2281 cgagtatcgg tttattctct gccatgtgtc atctcccccg ggttattctg gcacgctacc
     2341 gccatgcaga acatatggtt ccgcacagcc cggctggttc atgcgcttgc tccgggctcc
     2401 cttcggccgg ccgaggggag cccgtttcgg gccaaggcta ttccagacgc aaaatgcccg
     2461 gaatcagtat gcgctccttc tccatttcct tgagcaattg ctcatagctg agcaacggcg
     2521 aattcaattc agtcagcacg caaccgtcat acggctcaac ctgttgcagt gaatgccaga
     2581 cagaaacacc ggcgaagcgg cgtcgttcgg tatgcggatc caggatgccg acaatatcga
     2641 catccgtttc ctctgcgcgc agcatggcaa tctcggccaa gtccgacatt ccatacagca
     2701 gaatcttgcg ccatccgcgt gtctcgcagc ggtgaaaaac atccatgcag gattcaccgg
     2761 cacgccggta aaaggcaaaa gaagtggaca gatatttcgc ggtaagccgg cttttttccg
     2821 cgaatccctt gggcgtcaga tagtagaggt agcgattggc cggggcttcg ctgatcttga
     2881 tgaagccctt gcgaatgcag cgcttgagat aggaattcgc cagacccagg gcaatcccca
     2941 tattgttggc cagatggcgc tggctgacgt tgctttgctt gccgacggcg tccagcagct
     3001 ccagtacccg ctcttcttcc ctgtcggcct tcgtgttcat aatatgaaca ctatagccct
     3061 ccgccgggcc ggtcaaggac aatatcgggc cctaggcagg ttcaatcgag gcagaatcga
     3121 cggtggcgcg ggccttccgg cccagcacat ccagcaagac cgtcacccgg tcacggccgg
     3181 tggcagcctg aaaaatccct tcatatccgg caaaccctcc ctgggtaatt cgtacacggc
     3241 tgcccggctt gtactcttcc acggggagaa tctgtatgcc ctgcgtgtct tctcgtgacc
     3301 tgagcatggc tagcagctca tccggaacgc gcgcagccgc gcgaccaaat cttacgatgg
     3361 aaactacacc cagagtggaa cggatgggag cccagttatc agtctggcgg ttgaggtgga
     3421 tgaacaaata acgcgggaac atcggcgcga tcagggcgac gcgcttgccc tgacgccggc
     3481 gcacgtcgcg catgaagggt agataggttt cgtaaccctg acgcgcgaga ttttcgcggg
     3541 cgacggattc ctgacgcggc ttgcaataaa ccagatacca ggccctggca gcggaatcat
     3601 tcgtcgccgt gttcaattta ggtatcaaag tctccagaca tgcatgccgg catcggtaaa
     3661 tgccttggta ttgaagcgcg atttgacgtc aataaaacag cctccgcgct caagcttctt
     3721 cagatactca tctattgcca tgccaagaaa cggtttatgc gcaaccgcag ccacaatcgc
     3781 cttggccttg gccaatgatt cccatgaatg cagttcaatg ccgtattcgt gcctggcttc
     3841 ttcaacgtca gcgaccgggt
//
LOCUS       DBNGZE010000227         5000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1837427, whole genome shotgun sequence.
ACCESSION   DBNGZE010000227 DBNGZE010000000
VERSION     DBNGZE010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1837427"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1054
                     /locus_tag="AC16FV_06000"
     CDS             47..1054
                     /locus_tag="AC16FV_06000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2849645.1"
                     /translation="MNVLVTGASGFLGRHVVAALRDAGHRVRALVRPATDIGPLDWSS
                     DIDVFRADLCDADNLADAFDGIDVLVHLAAQVRGDDRTRISATLAGTERLLDAMAAGA
                     TKRLILASSFSVYDHRALRGTLGETDPVESSDVHQRDSYAIAKVEQERMVRRYAQQHS
                     RELIVLRPGIIWGPGHLDPPNLGQHLGPLYLIIAPRATLPLTYVENCAEAFAKTVTAA
                     AGQTLNVVDDELVSAWRFAGEYLRRTGQAAIRVPVPYRFGLLAGHLASAVQRIILFGK
                     PSLPNILRPRCFEARFKPLHYSNDAARRALGWRPRFTFDQAWDRALGPPSPRPAEASH
                     A"
     gene            1047..2282
                     /locus_tag="AC16FV_06005"
     CDS             1047..2282
                     /locus_tag="AC16FV_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2849646.1"
                     /translation="MPERRVIAYLTTVYARASDTFIRGEVEQLRAAGYTVHTFSIRRS
                     AGSELTSEDIRREQRNTVYILDHIPHLFFATLRTFLASPFRFLGVLGLAWRMAIPGIR
                     GRLWSIAYLMEACVLARHLRDRRARHLHNHLGQNSATVAMLASGLTGIPYSLTIHGPH
                     EFDEPLDLALNEKISRAAFTVAVSEYGRSQLFRWCEYAHWGKIHVVHCGVDSHFLDGP
                     KPLPPKAPRLVCIGRLAGEKGQLLLVEAVNCLKRQGIYCDLALIGDGPMRAPIERLIA
                     RHGLQDHVRIHGWLDADAVRQEILGSRALVLPSFAEGLPVVIMEALALERPVIATHIA
                     GIPELVEPRVNGWLVPAGSVPLLADAMKQALSLTPEQLHRMGQQGSHRVRQQHHAPTE
                     AAKLAALMEESVNALAPRQ"
     gene            2671..2904
                     /locus_tag="AC16FV_06010"
                     /pseudo
     CDS             2671..2904
                     /locus_tag="AC16FV_06010"
                     /inference="COORDINATES: protein motif:HMM:NF046806.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            2914..3261
                     /locus_tag="AC16FV_06015"
     CDS             2914..3261
                     /locus_tag="AC16FV_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849647.1"
                     /translation="MEIDDCWFNDARVYIRKLLTIRRRTFLSPIRSVFKLPGAPRQKL
                     VNTRGNRMDYAAGVAELAEALLHGRSCRLSPRFSLHVTEVGLALQDAGTSGCAYHARS
                     RFEPIPPLDWTRG"
     gene            3444..4673
                     /locus_tag="AC16FV_06020"
     CDS             3444..4673
                     /locus_tag="AC16FV_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017653691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR2849648.1"
                     /translation="MSDSQSSDQHPADRPVNIAILGCGYVADFYMATLGNHPNLNAIG
                     VYDSNPPRLAEFSRYYSIKPYKNLDDLLNDPSVELVLNLTNPRAHYDLTRVCLEADKH
                     VYSEKPLAMTSDKAAELVKLAKQKKLYLAMAPCSMLGETAQTMWKALRDNAIGPVRLV
                     YASFDDGMVHRFNPGGWRSASGAPWPAKDEFETGCTYEHAAYVLSWLAAFFGPARRVQ
                     SYASIRIRDKGIAVDSMAPDFTVGCIEYDNDVVARVTCSIVAPLDKSIVIVGEKGTLY
                     TKYVRNDASPVYIEKTPPNRIFSGITSRLKYLQVRFEELFHLPFSISPFRFEKKYPYA
                     CRPGFHRSGGNKPVDFLRGPAEVAGAIRENRPCRLSPELGLHMTELIETLQYPERFES
                     PRTINSRFDPIQPLPWK"
     gene            4729..>5000
                     /locus_tag="AC16FV_06025"
     CDS             4729..>5000
                     /locus_tag="AC16FV_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849649.1"
                     /translation="MKNAGNRTVIAYLAPETLAPWATFVYEELLELERRGLSVIPMSL
                     RRPSQTARGQEALAERMLIVYEGSTLGLLVQGLVGVPLFGLGAWKA"
BASE COUNT          936 a         1593 c         1396 g         1075 t
ORIGIN      
        1 ccgatgccct gggcacaata aatcacggaa catcatttag atttcgatga acgttcttgt
       61 cacgggagcc agcggattcc tcggccgaca cgtcgtagcc gccttgcgcg acgccggtca
      121 tcgggtacgc gccctggtgc gtccggcaac cgatatcggg cctctcgact ggtcttccga
      181 cattgacgtc tttcgcgcgg atctctgcga cgccgacaat ctcgcggacg ccttcgacgg
      241 aatcgacgtt ctggtccact tggccgccca ggtccgcgga gacgaccgga cccggatttc
      301 cgcgacgctt gccggcacgg aacggctgct ggatgcgatg gcggccggcg ccaccaaacg
      361 tctgatcctc gccagcagtt tttcggtcta cgaccaccgc gcgctccgcg gcacgcttgg
      421 cgagaccgat cccgtcgaat catccgatgt gcatcagcgc gacagctacg ccatcgccaa
      481 ggtcgagcag gagcgaatgg ttcgacgcta cgcccagcaa cactcccggg agctgatcgt
      541 gctgcgcccc ggcatcatct ggggacccgg ccatctcgat ccacccaacc ttggccagca
      601 tctcgggcct ctgtatctca tcatcgcccc acgcgcgacg cttccgctga cctatgtcga
      661 gaactgcgcc gaggccttcg cgaaaaccgt caccgcggct gccggacaga ctctcaacgt
      721 ggtggatgat gaactcgtga gcgcctggcg ctttgccggt gaatatctcc ggcgcaccgg
      781 gcaagccgcc atccgcgtcc ccgtacctta ccgtttcggg ctgctggcgg gccatcttgc
      841 cagtgccgtg cagcgcatca ttctattcgg caaaccttct ctccccaaca ttctccgccc
      901 tcgttgcttc gaggcccgtt tcaagcccct ccattactcc aacgacgcgg ctcgccgggc
      961 tctggggtgg cgcccaagat tcacctttga tcaggcctgg gaccgggctc tgggcccgcc
     1021 gtccccgcgt ccggcggagg cctcccatgc ctgagcggcg cgtcatcgcg tacctcacca
     1081 ccgtctacgc ccgtgcttcc gatacgttca ttcgtggcga ggtcgagcag cttcgcgccg
     1141 ccggttacac cgttcacacc ttttctatcc gacgcagcgc ggggtcggaa ctgaccagcg
     1201 aggatatccg gcgcgaacag cggaacaccg tatacatcct cgaccacatt ccccacttgt
     1261 tcttcgccac gctccggact ttcctggcgt caccgttccg tttcttgggc gttctcggcc
     1321 ttgcttggcg catggccatc cccgggatca gaggccgcct ctggtccatc gcctacctca
     1381 tggaggcctg tgtcctggcc cgccacctgc gagacaggcg cgcgcgccac ctgcacaacc
     1441 atctcggcca gaactcggcg accgtcgcca tgctcgccag cggtctcacc ggcattccct
     1501 acagcctcac gatccatggt ccccacgagt tcgacgagcc gcttgatctg gccctgaatg
     1561 aaaaaatctc ccgcgccgcg ttcaccgtcg ccgtatccga gtacggccgc agccaactct
     1621 tccgctggtg cgaatacgct cactggggca agattcacgt ggttcactgc ggcgttgact
     1681 cacactttct tgatggaccc aagccgcttc caccgaaggc gccccgtctc gtctgcatcg
     1741 gacggctcgc cggggaaaaa ggccagctgc ttctcgttga agccgtcaat tgcctcaagc
     1801 ggcaaggcat ttattgcgat ctggccctga ttggcgacgg tcccatgcgc gcacctatcg
     1861 aacgcttgat cgcacgtcac ggcctgcaag atcacgtccg cattcacggc tggctcgatg
     1921 ccgacgctgt acgacaggaa atcctcggtt cccgtgccct tgtccttccc agctttgccg
     1981 aaggtctccc ggtcgtcatc atggaagccc tcgccctcga gcgccccgtc atagcgaccc
     2041 acatcgccgg tatccccgag ctcgtcgaac cccgcgtcaa cggctggctc gtccctgccg
     2101 gctcggtacc gcttctcgcg gacgccatga agcaagccct ctctcttacg cccgaacagc
     2161 tgcaccgcat gggccagcag ggctcccatc gcgttcgtca gcaacatcat gctcccaccg
     2221 aggccgcgaa actcgccgcg ctgatggaag agtcggtgaa tgccttggca ccacgacaat
     2281 agtgtcggct ctctttgtga ggcacctcgg gtcggcagtt tctgcgccga ccgcgggccg
     2341 gacggcaaat gggacgcgcg tcgtccgccc gcgttgactg atgccggatg cgtcgtgtca
     2401 cgccggctca tcaacgccac atggcagacg gttacgtttc gctccctgcg tcgctttacc
     2461 gtgtcattaa atattattat cgtagtgccg cggccctcag taaacgtttg gcacgtcccg
     2521 tgtgccaaac gccttcggtc tcgcggcgta ttttttgtcg cgaccgtcct gcggctgaga
     2581 ggcgccacaa tgactctacg ccgcaggtag tgcggctatc gcgggctctt cgccagcgct
     2641 tcgctttccc tggctcatcg tggttgcacg ctggagcacg ctggatacta tctgagctgg
     2701 ctgattgcat tcttcggtcc tgttaaaacc gtcaccgcct attccgactg cctgatttca
     2761 gaaaaaataa aagacgataa actcgaccct gcaaacacac ccgatttttc cgtgggcttg
     2821 ttaaaattcg agtctggcat cgtagcgcgt ctgacgacaa ccattgtcgg tccccatgac
     2881 cattccattc gtgtcattgg ataccggggc gtcatggaaa tcgacgactg ttggttcaac
     2941 gatgcgaggg tatatattcg aaaattgctt acgatccgtc gcaggacttt cctgtcgccg
     3001 atccggtcgg tgttcaagtt gcccggcgca cccagacaga agctggtcaa cacgcgcggc
     3061 aatcggatgg actacgccgc cggcgtcgcg gagttggctg aggccttgct gcatgggcgt
     3121 tcctgccggc tgtcaccgag gttctcgctt cacgtcacgg aggtgggctt ggcgttgcag
     3181 gacgcgggga catcaggctg cgcgtaccat gcgcgatcca gatttgaacc catcccgcca
     3241 ttggattgga cccggggctg agagcagcgc cccctggaaa acctggttgc cggtctcacg
     3301 gcacagggca ggcgacttcg tgtggccatg ctggtacccg ttcacgacaa ggcggccgtg
     3361 attccggtga gtcacaagat tcgttatgtt tgccgggaaa ttttggttat catgcctcgc
     3421 agccaaattt ttgaatcacg ctaatgtcag actcccaatc atcggatcag catcctgccg
     3481 atcggcccgt taacatcgcc attctgggtt gcggctatgt cgccgatttt tatatggcga
     3541 cgctgggaaa ccaccctaat ctgaacgcca tcggtgtata cgatagtaat ccgccacggc
     3601 tcgcggaatt ctcgcgatat tacagcatca agccgtataa aaatctggac gacttgttga
     3661 atgacccctc ggtggagtta gtgctcaatc tcaccaaccc gcgtgcgcat tatgatctca
     3721 ccagggtctg cctcgaggcg gacaagcatg tctattcgga gaagccgctg gccatgacgt
     3781 ccgacaaggc ggcggagttg gtgaaactgg ccaagcagaa aaagctttat ctcgccatgg
     3841 cgccatgcag catgcttggc gaaacggctc aaacaatgtg gaaggctctc cgtgataatg
     3901 cgatcggccc tgttcgattg gtttatgcca gctttgatga cggcatggtg caccgcttta
     3961 accccggcgg ctggcgaagc gcttcaggag ccccatggcc tgcgaaagat gaatttgaaa
     4021 caggttgcac ctatgaacat gccgcctatg tcctgtcatg gctcgcggca ttcttcggcc
     4081 cggcaagaag agtgcagtcc tatgcctcga tccggatccg agataaaggc attgccgtcg
     4141 actccatggc tccggatttt acggtcggct gcatcgaata tgataatgac gtggtggcgc
     4201 gggtcacttg cagtattgtc gccccgctgg acaagtccat agtcattgtg ggcgagaagg
     4261 gtacgctcta caccaagtat gtcagaaacg atgcatcgcc ggtttatatc gaaaaaacgc
     4321 cgcccaatag aattttttcc ggcatcacca gccgcctgaa atacctgcag gtgcgctttg
     4381 aggagttgtt ccatctgcct ttttccatca gcccgttcag gttcgagaaa aaatatccct
     4441 atgcatgcag gcccgggttt catcgctcgg gcggcaataa accggtggat tttctgcgtg
     4501 gccccgccga ggtggcggga gcaattcggg aaaataggcc ctgccggttg tcgcccgagc
     4561 tgggtctgca tatgacggaa ttaatcgaaa cgctgcaata cccggaaaga ttcgagagtc
     4621 cacggacgat caactcccgc ttcgatccca ttcagccgtt gccctggaag taagcggccg
     4681 gtacccggga atcagctgat attctattag cgacagcgac tcctgatcat gaagaacgcc
     4741 gggaaccgga ccgtgattgc ttatcttgcg cctgaaacac tggcgccatg ggcaaccttc
     4801 gtctacgaag agcttctcga actcgagcgt cgcggactgt cggtgattcc catgtcactg
     4861 cgtcggccgt cccaaacggc gcgcgggcag gaagcgctgg cagagcgaat gctgattgtt
     4921 tacgaaggtt cgacgctggg cctgctggtc cagggtctgg ttggggtgcc actcttcggt
     4981 ctaggtgcat ggaaggcgtt
//
LOCUS       DBNGZE010000228         4798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1837435, whole genome shotgun sequence.
ACCESSION   DBNGZE010000228 DBNGZE010000000
VERSION     DBNGZE010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1837435"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16FV_06030"
     CDS             <1..609
                     /locus_tag="AC16FV_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR2849650.1"
                     /translation="PMADRDDARSEFNPKHRIVGAIVLVALVVIFVPMILSEREPPPE
                     LKGMREPQPRTETAETKVVVTPVPTEEVKPKESSEAVKVVQVPVEPKPKPVPETKPAL
                     PAEKPAEAKKPEPAPAKPAAKIENGWMVQVGTFSNTQNALRLRDRLKSLGHSVHTDSI
                     TVSGKKALRLRVGPFADRAKADKAQAQIRKETGVNGVVQSQT"
     gene            614..1108
                     /locus_tag="AC16FV_06035"
     CDS             614..1108
                     /locus_tag="AC16FV_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR2849651.1"
                     /translation="MNSFDLLIIGILAIFAAIGAWRGLLSELISMLTWVLSVVLAWLY
                     AAPLSRFFKGLIEDIALRQLTAFALIFMLVFVLGLVASWLIHKYFPAKRAFRIANTAL
                     GGLVGAARGGVVVIVVFLVAGLTPFPQRDWWRESAIAPVFERAAVYVAGYIPRDIAQH
                     LRYG"
     gene            1142..2668
                     /gene="purF"
                     /locus_tag="AC16FV_06040"
     CDS             1142..2668
                     /gene="purF"
                     /locus_tag="AC16FV_06040"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR2849652.1"
                     /translation="MCGIIGILAKSPVNQAIYDGLLVLQHRGQDAAGMLTCDGNRIHL
                     RKDNGQVRDVFQERHMLGLKGNMGIGHVRYPTAGCESPAEAQPFYVNSPYGLALAHNG
                     NLTNADQLKDELYREDLRHINTDSDSEILLNVFAHELQAVAKLRLAPEHIFKAVSAVH
                     RRCRGAYAVTMLVTGFGILAFRDPYGIRPLVYGKRETDQGTEYMFASESVALDVVGFD
                     LVRDVAPGECIMVDVNGQVHSQQCAEKPIYAPCIFEFVYFARPDSMMDGIYVHKTRLR
                     MGERLAGKIKRVWPDHDIDVVIPIPDTSRTAALQMVYELDLPYREGFIKNRYIGRTFI
                     MPGQAERKKSVRQNLNAIDVEFRRKNVLLVDDSIVRGTTSKQIIQMARDAGARKVYFA
                     SAAPPVRFPNVYGIDMPSRNELIGNGRTEDEIAAVIGADRLIYQDLEDLIEAAREGNP
                     KIQKFDASCFNGEYITGDVDRAYLDRIENERNDSAKSSRSVSQNSNDLVTAEVLTFPR
                     "
     gene            3085..3714
                     /locus_tag="AC16FV_06045"
     CDS             3085..3714
                     /locus_tag="AC16FV_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849653.1"
                     /translation="MKFTAPNTAEQYFVITEHAVRHLYAGLDSCWSYYKSALNHWDVS
                     KVGQPLTKENKEALQKYLDLAKKYFDLKFSEAMFAGGILQIAFMAIKLYSSNNSIPSS
                     CTNLVKQKHKTAIPFCIGREYYGIPVGLIIYASRNQYSHWDDEEPHEVTRNVFDALSA
                     AFQNNMLADLAFELSNPAINIYANEVLLTAMKWMSYDIYLHEMKLTLQS"
     gene            3738..4655
                     /locus_tag="AC16FV_06050"
     CDS             3738..4655
                     /locus_tag="AC16FV_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR2849654.1"
                     /translation="MEHVAAWRGKVIVAFAAVYIIWGSTYLAIRVGVQQLPPALFAGS
                     RFVVAGILLALYARLSGQKFPTHLREWRTIAITALFMLVGANGLVVWGEQWVPSNQAA
                     LIVATTALWLAGFGTLGAHGQKLGPRTVLGLTVGFFGVAMLMLPENGFSLEHFGAQLA
                     VLAACPLWAAGSMVAKRSHLSTPPLMVAAMQSLVAGIVFCGFGFTAGETVRWSWSPLA
                     MAALAYLIVFGSCFAYAAYVWLLHEVSPAALGTYAYVNPLVAVVLGWLILDETLGAAQ
                     MAGMVVILIGVMLVSIARPKALAAPNQRT"
BASE COUNT          973 a         1438 c         1383 g         1004 t
ORIGIN      
        1 cccatggcgg accgagacga cgcccgttcc gaattcaacc ccaaacaccg catcgtcggc
       61 gcgatcgtgc tggtcgcgct ggtggtgatt ttcgtgccca tgatcctcag cgagcgcgag
      121 ccgccgccgg aactgaaagg tatgcgcgag ccgcaaccac gcacggaaac cgccgagacc
      181 aaagtcgtcg tcacgccggt acccacggaa gaggtgaaac cgaaggaaag cagtgaagcg
      241 gtgaaagtcg tgcaggtgcc ggtcgagccc aagcccaagc ccgtgccgga aaccaaaccc
      301 gctctgcctg cggagaaacc ggccgaggcg aaaaaacccg agccggcccc ggccaaaccc
      361 gccgctaaaa tcgaaaatgg ctggatggtg caggtcggaa ccttctccaa cacacaaaac
      421 gccttgcgtc tgcgcgacag gcttaaaagc ctggggcatt ccgtgcacac cgattcgatc
      481 acggtcagcg gaaagaaggc attacggctg cgcgtcggcc cgttcgccga tcgcgccaag
      541 gccgacaagg cgcaggcaca gatccgcaag gaaaccggcg tgaacggcgt ggtgcagtcg
      601 caaacctgac gctatgaaca gtttcgatct gctgattatt ggaattcttg cgatcttcgc
      661 cgccatcggc gcgtggcgtg gcctgctgag cgagttgatc tcgatgctga cgtgggtgtt
      721 gtccgtggtt cttgcctggc tttatgccgc gccgttgtca cgatttttca agggattgat
      781 agaagacatc gcgctgcgcc agctgaccgc cttcgcgctg attttcatgc tcgtgttcgt
      841 gctgggtctg gtagcctcgt ggctgatcca caaatatttc cccgccaagc gcgccttccg
      901 catcgccaat accgcgttgg gaggtttggt cggcgcggcg cgcggcggcg tggtagtcat
      961 cgtcgtgttc ctcgttgccg gcctgacgcc gttccctcaa cgcgactggt ggcgcgagtc
     1021 cgccattgcg ccggttttcg agcgcgcggc ggtctatgta gccggttata tcccgcgcga
     1081 tattgcccag cacctccgct acggctaaaa tcgcgtttta agtacaggag attccttcgg
     1141 catttgcgga atcatcggta ttctcgccaa atccccggta aaccaggcca tctatgacgg
     1201 cctgttggtc ctgcaacacc gcggccaaga cgccgccggc atgctcacct gcgacggcaa
     1261 tcgcatccac ctgcgcaagg acaacggcca agtgcgcgac gtgttccagg aacgccacat
     1321 gctcggcctc aagggcaaca tgggcatcgg tcatgtgcgc tatcccaccg ccggctgcga
     1381 gtccccggcc gaggcccagc cgttttacgt gaactctcct tacggtctgg cgctggcgca
     1441 taacggcaac ctgaccaatg ccgaccagct gaaggatgag ctgtaccgcg aggacctgcg
     1501 gcacatcaac accgactccg attccgaaat cctgctcaac gttttcgccc atgaattgca
     1561 ggccgtcgcc aagctgcgtc tggccccgga acatattttc aaggccgtgt ccgccgtgca
     1621 ccggcgttgc cgcggcgcct acgccgtcac catgctggtc accggcttcg gcatcctcgc
     1681 gttccgcgat ccctacggca tccgcccgct ggtatacggc aagcgcgaga ccgaccaggg
     1741 cacggagtac atgttcgcct ccgagtccgt cgcgctcgac gtggtgggat tcgacctggt
     1801 gcgcgacgtc gcgccgggcg agtgcatcat ggtggacgtg aacgggcagg tgcacagcca
     1861 gcagtgcgcc gagaagccaa tatatgcccc atgcattttc gaattcgttt attttgcgcg
     1921 ccccgactcg atgatggacg gcatctacgt gcacaagacg cgcctgcgca tgggcgagcg
     1981 cctggccggc aagatcaagc gtgtctggcc cgaccacgac atcgacgtgg tcatcccgat
     2041 ccccgacacc tcgcgcaccg cggcgctgca gatggtctat gagctcgacc tgccataccg
     2101 cgaaggcttc atcaagaacc gctacatcgg ccgcaccttc atcatgcccg gccaagccga
     2161 gcgcaagaaa tcggtgcgcc agaatctgaa cgccatcgac gtcgagttcc gccgcaagaa
     2221 cgtgctgctg gtggacgatt ccatcgtgcg cggcacgacc agcaagcaga tcatccagat
     2281 ggcgcgcgat gccggcgcgc gcaaggtgta tttcgcctcg gccgcgccgc cggtgcggtt
     2341 cccgaacgtg tacggcatcg acatgccgtc gcgcaacgaa ctgatcggca acggccgcac
     2401 ggaagacgag attgcggcgg tcatcggcgc cgacaggctc atctaccagg accttgagga
     2461 cctgatcgag gccgcgcgcg agggcaatcc caagatccag aagttcgatg cctcctgctt
     2521 caacggtgag tacatcaccg gagacgtgga tcgggcttat ctcgatcgca tcgagaatga
     2581 gcgcaacgat tccgccaaga gcagccggag tgtttcccag aacagcaacg atctggtcac
     2641 ggcggaagtg ttgacgtttc caaggtaatt tttctttttt gtcattcccg cgaaagcggg
     2701 aatccagggt ttgaataaca aaagccgcct taaggcggct ttttcatttg tgtcgccttc
     2761 ggcgacgatg aaaagctaag ggcggtttcc gcaccgcggg caggtccatt tcttttgttc
     2821 ggccaaaaga aatggacgaa agaaaaggcc gcccccgagt ctgcgccggc ttcgccggtt
     2881 ccctcacccc gtcggtcgcc tcagcgggtg cgcatacggc gcatccctgc gccggatgcg
     2941 cacgctcgcg atccctcgct cgccccttcg ggccggccct tcggcttccc tcggagttcg
     3001 gcttgactca gggggtggtt agcacaacca tgccggagtt atgcggtggc tttaaaatcg
     3061 aatttatagt caggtattca agaaatgaaa tttacagctc cgaacactgc cgagcagtac
     3121 tttgtaatta ctgaacatgc tgtacgacat ctttacgctg gcctggattc atgttggtcc
     3181 tactacaaaa gcgccttgaa ccattgggat gttagtaaag taggacagcc actaacaaaa
     3241 gaaaacaaag aagctctaca aaagtactta gatctagcaa aaaagtactt cgatctaaag
     3301 ttttctgagg caatgtttgc gggtggcatt ttgcaaatcg cctttatggc cataaaactc
     3361 tactcatcta ataactcaat tccgagcagc tgtacaaatt tggttaaaca gaaacacaag
     3421 acagcaatac cattttgtat tgggcgagag tattacggca ttcctgttgg gctaattatt
     3481 tatgctagta gaaatcaata tagtcactgg gacgatgagg agcctcatga agttacgcgg
     3541 aatgtttttg acgccttatc ggccgctttc caaaataata tgttggcaga tttagcgttc
     3601 gaattatcaa atcctgctat caatatttac gctaatgaag ttctgcttac ggcaatgaaa
     3661 tggatgtcat acgacattta cttacatgag atgaaattaa ctttacaatc gtaaagatta
     3721 agaaaaagta aaataacatg gaacacgtcg ccgcctggcg cgggaaagtc atcgtcgcct
     3781 tcgcggcggt gtatatcatc tggggctcga cttatctcgc gatccgtgtg ggcgtgcagc
     3841 aattgccgcc ggcgctgttt gccgggtcgc ggttcgtggt cgccgggatc ctgctggcac
     3901 tgtacgcgcg cttgagcggg cagaaatttc caacgcactt gcgcgaatgg cgcaccatcg
     3961 ccatcacggc gctgttcatg ctggtcggcg ccaacggcct ggtggtgtgg ggcgagcagt
     4021 gggtgccgtc gaaccaggcg gcgctgatcg tcgccaccac ggcgttgtgg ctggcgggct
     4081 tcggaacctt gggcgcacac ggccagaaac tgggaccgcg caccgtgctg gggctcaccg
     4141 tcggtttttt cggtgtcgcc atgctgatgt tgccggagaa cggcttctca ctggagcatt
     4201 tcggcgcgca gctcgcagtg ctggcggcct gcccattgtg ggccgccggt tccatggtgg
     4261 ccaagcgcag ccatctctcc accccgccgc tcatggtcgc ggccatgcaa tcgctggtcg
     4321 cgggtattgt tttctgcgga ttcggattca ccgccgggga aaccgtgcgc tggagctggt
     4381 cgccgctggc gatggccgcg ctcgcttatc tgatcgtgtt cggttcctgt ttcgcctacg
     4441 ccgcgtatgt gtggctgctg cacgaagtca gccccgcggc gctcggcact tacgcctacg
     4501 tcaatccgct cgtggccgtg gtgctcggct ggctaatcct cgacgaaaca ctcggcgccg
     4561 cgcagatggc aggcatggta gtaatattga tcggcgtcat gctggtgagc atcgcgcgcc
     4621 ccaaggcact ggcggcgccc aaccagcgga cttgataata cctaccggct gacgccgcac
     4681 tgtataattc gttgtccctt tccgttcctg tccgaccatg cagcattttg cctcgcttcc
     4741 gccgctgtcg ctctacgtgc acatcccctg gtgcgtgcgc aagtgtccgt actgcgat
//
LOCUS       DBNGZE010000229         1750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_309454, whole genome shotgun sequence.
ACCESSION   DBNGZE010000229 DBNGZE010000000
VERSION     DBNGZE010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_309454"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..209
                     /locus_tag="AC16FV_06055"
                     /pseudo
     CDS             1..209
                     /locus_tag="AC16FV_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422975.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="formamidopyrimidine-DNA glycosylase"
     gene            complement(147..>1750)
                     /locus_tag="AC16FV_06060"
     CDS             complement(147..>1750)
                     /locus_tag="AC16FV_06060"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR2849655.1"
                     /translation="GGDQSIRLITLEEATALFIGRLFPGYTVKGQGAFRAIRDSEVEI
                     EEEAEDLVRLYETALKQRRRGSVIRLELGASMPGELCNFVQRALAVADDEVFRVDGVL
                     ALNDLNQLTRLLDRPDLEFVPYNPRYPERIRDHGGDCFAAIRQKDLVVHHPYESFDVV
                     VQFLQQAARDPNVVAIKQTLYRTSAESPIVKHLVEAAEAGKSVTALVELKARFDEEAN
                     IRWARDLERAGAQVVYGFIELKTHAKLSLVVRREAGELATYVHVGTGNYHPVTARIYT
                     DLSFFTADAVIGRDVARIFNYITGYAEPGELERMAISPINLRKRILNHIAQEIAHAKV
                     GRPSSIWMKMNALVDPGIIDALYEASAAGVSVDLIVRGICCLRPGVPGLSKNIRVKSI
                     IGRFLEHGRIYCFGAGHPLPHPKAAIYISSADMTPRNLDRRVEVLCPILNATVHEQVL
                     GQIMVANFKDNEQSWTLLPDGSSTRIKAVPGEEPFNAHKYFMTNPSLSGRGKSLKESP
                     PRSLIRRLIQARDFGVRQSRAECQR"
BASE COUNT          366 a          523 c          508 g          353 t
ORIGIN      
        1 tcgaggtgct cgaagccgat cggccggcgt tcgccgccgc gctgcgccgc gagaaccaca
       61 cgctcaagcg cagcctgacc gacccccggc tgttctcggg tatcggcaac gcgtattccg
      121 atgagattct gcacgccgcc cgcctttcac cgctggcact cagcgcgaga ctgacggacg
      181 ccgaagtcgc gcgcctgtat gaggcgacgt ataaggctgc gcggcggcga ttccttgagc
      241 gattttccac ggcccgacag acttggattt gtcatgaaat atttatgggc gttgaagggt
      301 tcttcgcccg gcacggcctt tatgcgcgtg gaactgccat ccggcaagag cgtccagctt
      361 tgctcgttat ccttgaaatt ggcgaccata atttggccga gaacctgttc gtgcaccgtg
      421 gcattgagaa tcggacacaa aacttcgacc cggcggtcga gattgcgcgg cgtcatatca
      481 gctgaggaaa tataaatcgc ggccttcgga tgcggcagtg gatggccggc gccaaaacaa
      541 tagatgcggc catgttctag gaaacgccca ataatcgatt tgacgcggat attcttggag
      601 agccccggca cacccggccg cagacagcag atgccgcgca cgatcaggtc aactgaaacc
      661 cccgcagccg atgcttcgta aagcgcgtcg atgattcccg gatcgaccaa cgcgttcatc
      721 ttcatccaga ttgacgaagg ccgcccgacc ttggcatgcg cgatttcctg cgcgatatga
      781 ttgaggatgc gcttgcgaag gttgattggc gaaattgcca tgcgttcaag ctcacccggc
      841 tcagcgtagc cggtgatata attgaatatg cgcgcaacgt cgcggccgat gactgcatca
      901 gcggtaaaaa atgaaagatc ggtatagatg cgtgcggtga ctggatgata attcccggta
      961 ccaacatgca cgtaggtcgc tagctccccc gcttcgcgac gcaccaccaa tgataacttg
     1021 gcgtgcgttt taagttcgat aaagccatac acgacctgcg cgcccgcacg ttcgaggtcc
     1081 cgtgcccaac ggatattggc ctcctcgtcg aagcgggcct taagctccac cagtgcggta
     1141 acagattttc cggcctcggc cgcttccacc aggtgcttca cgatcggcga ttcagccgaa
     1201 gtgcgataga gcgtctgctt gatcgcgacc acgtttgggt cgcgagcggc ctgctgcagg
     1261 aactgcacta ccacatcgaa agattcgtag ggatggtgga ccacgaggtc tttttggcga
     1321 atagcagcaa agcaatcgcc gccgtggtcg cgaatgcgtt ccggataacg cggattgtag
     1381 ggcacgaatt cgagatcggg gcggtcgagg agcctggtga gctggttgag atcgttgagc
     1441 gcaagcacgc catcgaccct gaacacttca tcgtcggcga ccgcgagcgc gcgctgtacg
     1501 aagttgcaca actctcccgg cattgaggca ccgagttcca gccggatcac cgaaccgcgc
     1561 cgccgctgtt tgagcgcagt ctcgtataac cgcaccaggt cttcagcctc ttcctcgatt
     1621 tccacttcgg agtcgcggat ggcgcggaat gcgccctgcc ccttgaccgt gtagccggga
     1681 aacagccggc cgataaacag cgccgtcgct tcctccagtg taatcagccg gatactctga
     1741 tcgccgccac
//
LOCUS       DBNGZE010000230         4626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_309975, whole genome shotgun sequence.
ACCESSION   DBNGZE010000230 DBNGZE010000000
VERSION     DBNGZE010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_309975"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="AC16FV_06065"
     CDS             <1..235
                     /locus_tag="AC16FV_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HIR2849656.1"
                     /translation="ISSYRQAAMLNPDGSHGPVKCAACHTVNKYGVPSRYQELIGKQG
                     PAWNNYDKAVELQHLLRAGAAAPRRDNAQKQSE"
     gene            258..779
                     /locus_tag="AC16FV_06070"
     CDS             258..779
                     /locus_tag="AC16FV_06070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2849657.1"
                     /translation="MKRLLVMWLLCLAPLMPALAAGKIAFSLLDTDGKVHTLSQYTGK
                     WVVINYWATSCPPCIKEIPQLNMFHKKHKDQDAVVLGVDFEDISLAWLKDFMDSMSMS
                     YPVLRSDPSQQVTPFGVIIMLPTTFIISPTGELVAHQAGAVTAADLEAFIKRQKESGQ
                     ANAPAIKINTKIR"
     gene            974..1258
                     /locus_tag="AC16FV_06075"
     CDS             974..1258
                     /locus_tag="AC16FV_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR2849658.1"
                     /translation="MTQAIWNGAILAESDHCKVVEDNYYFPPESVRRDHLRPSTKHST
                     CPWKGVASYYDVMVDGKVNCKAAWYYPQPREAARDIKDYVAFWHGVQVVP"
     gene            1331..1546
                     /locus_tag="AC16FV_06080"
     CDS             1331..1546
                     /locus_tag="AC16FV_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR2849659.1"
                     /translation="MTTITLRIDDKLDAQLKRMSKRTGRPKSELIREALRRQLALTQF
                     EKLRQTAAPFAEAKGWLTDEDVFRELS"
     gene            1543..1944
                     /locus_tag="AC16FV_06085"
     CDS             1543..1944
                     /locus_tag="AC16FV_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR2849660.1"
                     /translation="MRVFLDTNVLVAAFATRGICADLFRAVLLEHDLITGEPVLREIH
                     RVLVRKLKFPESQTREIILFLRNHAEVTNPKKAANWPEADADDRWIVAAAIEGKAEIL
                     VTGDKDLLEARTQTGIRVVTPRGFWELLKAE"
     gene            2340..>4626
                     /locus_tag="AC16FV_06090"
     CDS             2340..>4626
                     /locus_tag="AC16FV_06090"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIR2849661.1"
                     /translation="MAIKKSELYSSLWSSCDELRGGMDASQYKDYVLTLLFVKYVSDK
                     YAGQKDALLTVPKGGGFVDMVAAKNDKEIGEKLNKIINKLADANESLKGAINVADFND
                     EDKLGKAQAMVDRLTKLVAIFEGLEFGKNRAEGDDLLGDAYEYLMRHFATESGKSKGQ
                     FYTPAEVSRVMSQVIGLGQATRASQSIYDPTCGSGSLLLKAHDEAKSRTGLDLALYGQ
                     EMDNATSALARMNTVLHDCPTAEIWQDNTLAHPHFKQNETTLKTFDYVVANPPFSFKA
                     WSSGFNPANDLYGRFEYGIPPVKNGDYAFLLHILKSLKSSGKGAVILPHGVLFRGNVE
                     ADIRTRIVKQGYIKGIIGLPANLFYGTGIPACIVVLDKENAAARKGIFMIDASKGFIK
                     DGNKNRLRAQDIHKIVDTFTKQLEIDKYSRLVPLAKIEAEGYNLNLPRYIDSTEPEDL
                     QSIEAHLNGGIPVADIEALQSYWDICPNLRTALFKPNRPGYLDLVVEKGAIKPAIYEH
                     PEFTAFTAGMNTHFDVWRAKVAKTLKALKAGFRPKEMIVSFSENLLAHYKDKPLIDHY
                     DVYQHLMDYWAETMQDDCYLVAAEGWLVGNRVREIIQVKNKDGKLVWPESHDYKRGKR
                     RFKSDLIPAAVLINRYFKPEQEGIDVLESAVAALEQQLDERREEQGAEGGLLEEVVEG
                     EGDKQKITAKAVKARLKEIGTDAAFADEQTALREYADLLEKLTETKSQLKQAQDDLDA
                     KLHAKYPKLTEAEIKTLVVDDK"
BASE COUNT         1073 a         1384 c         1319 g          850 t
ORIGIN      
        1 tatttccagc taccggcagg cggcgatgct gaatcccgat ggttcccatg gcccggtgaa
       61 atgcgcggcc tgccatacgg tcaacaagta cggtgtgccc tctcgctacc aggagctgat
      121 cggtaagcag gggccggcat ggaataacta tgacaaagct gtcgagctcc agcacctgct
      181 gcgagcgggc gccgcagcac cacgacgtga taacgcgcag aagcagtcag aataacgctc
      241 cgtgagcaga aactgaaatg aaacgacttc ttgtcatgtg gctgctttgc ctggcgccgc
      301 tgatgccggc gctggccgcc ggcaagattg ccttttcact gctggacacg gatggcaagg
      361 tccacacgct gtcgcaatat accgggaagt gggtggtcat caattattgg gcgacctctt
      421 gcccgccgtg catcaaggag attccgcagc tcaacatgtt tcataaaaag cacaaggatc
      481 aagacgccgt cgtgctgggc gtggactttg aggacatttc cctggcttgg ctgaaagact
      541 tcatggacag catgtccatg agctatccgg tgttgcggtc ggatccatcg cagcaagtca
      601 cgcccttcgg cgtcattatc atgttgccta ccacctttat catttctccg accggcgaac
      661 tcgtggcgca ccaagcgggc gccgttaccg ccgccgacct cgaagccttc atcaagcgcc
      721 agaaggagtc cggccaggcg aacgcgcccg cgattaaaat caacactaaa attcgttagg
      781 gaagctctga ttaagtccgt tcgtggtgag cctgtcgaac catgaacgga caaatcgcct
      841 gtaacttacg gcatttcctt tcaccccctt cgactggctc aggacaggct tcgacaagct
      901 cagggcgaac gggagactta atcagagctt ccttagcctg tcccgggcac cattcctgac
      961 gggagactgc cgcatgacac aagccatctg gaacggcgcg atcctggccg agagcgacca
     1021 ctgcaaggtc gtcgaggaca actactattt cccgcctgag tcggtgaggc gcgatcatct
     1081 gcgcccgagc accaagcaca gcacctgccc gtggaagggt gtggcgagct attacgacgt
     1141 gatggtcgac ggcaaggtga actgcaaggc ggcgtggtat tacccgcaac ccagggaagc
     1201 cgcgcgcgac atcaaggact atgtcgcttt ctggcacggg gtgcaggtcg tgccgtaggg
     1261 cgcgcgccgc gcgccattca tcccaaccgc gggcttgcgg ttcaggcgtc ttcgtattac
     1321 attgtaatac atgaccacga ttacccttcg catcgacgac aagctggatg cccagttgaa
     1381 acgaatgagc aaacgcaccg gccggcccaa aagcgagctg attcgcgagg cgctgcggcg
     1441 ccaattggcg cttacccaat tcgagaaact gcgtcagacg gcggcaccgt tcgccgaagc
     1501 gaaaggttgg ctgaccgatg aggatgtgtt ccgcgaactt tcatgagggt gttcctggac
     1561 acgaacgtcc tcgtggccgc ctttgccacg cgcggtatct gcgcagatct tttccgggcc
     1621 gttctgctgg aacatgacct gataaccggc gagccggtac tgcgggaaat tcaccgtgtg
     1681 ctggtccgaa aactcaagtt cccggaatcc caaacccgcg aaatcatcct cttcctgcgc
     1741 aatcacgctg aagtcaccaa tcccaaaaaa gcggccaact ggccagaagc agacgcggat
     1801 gaccgctgga tcgtggcggc tgcgatcgaa ggaaaagccg aaatactcgt gacaggggac
     1861 aaggatttgc tggaagcaag gacgcagacc ggtattcgtg tcgtgacacc gcggggtttc
     1921 tgggaactgc tcaaggcaga gtagagcccg ttggggttcg gcgtttacgc ctcaacctgc
     1981 ctgaagacct gccttgaaag gtccggtgaa cctttctccc gccgaacggg cgcaccacct
     2041 agcatgtcgt ccccgcgaag gcggggaccc aggacttcta aaaaacgcct ttcccaccgc
     2101 atcagccgtg cccaggaatt tccagccgtc gtcacccccg cagcctcgcc ccacgggagt
     2161 cttatgttag gattccagca taaatagaag ggattggagg agggcgcttt ggttctacag
     2221 gtagctcaag aagttgcttc gttgtggctt gctttgcctt cctctcgtca ttgcaatcta
     2281 tctgagtcat taacagtcca accatccaat atttccggcc gccctggacg cggaatacca
     2341 tggctataaa aaaatccgaa ctctatagct ccctctggtc cagctgcgat gaactgcgcg
     2401 gcgggatgga cgcctcacag tataaggact acgtcctaac gttgctgttc gtgaaatatg
     2461 tctccgacaa gtatgccggc cagaaggacg cgttgctcac tgttcctaag ggtggcggtt
     2521 ttgtcgatat ggtggccgcc aagaacgaca aggagatcgg cgaaaagctc aacaagatca
     2581 tcaataaatt ggccgatgcc aacgaaagcc tgaagggcgc gatcaacgtc gccgacttca
     2641 acgacgagga caagctcggc aaggctcaag ccatggtcga tcggcttacc aaactggtag
     2701 cgattttcga ggggctggag ttcggcaaga accgcgccga gggcgacgat ctgctgggcg
     2761 acgcctacga gtacctcatg cgccacttcg ccaccgagtc cggcaagagc aagggccagt
     2821 tttatactcc ggcggaagtc tcccgcgtga tgtcgcaggt gattggtctg ggccaggcga
     2881 cccgcgccag tcaaagcatc tacgacccca cctgcggttc cggttcgctg ctgctcaagg
     2941 cgcacgacga ggccaagagc cgcaccgggc tagacctcgc cctctacggt caggagatgg
     3001 acaacgccac ctcggcactg gcgcgcatga acacagtgct gcatgactgc cccaccgcgg
     3061 aaatctggca ggacaacacc ctggcccacc cgcacttcaa gcagaacgaa accaccctca
     3121 agaccttcga ttacgtcgtc gcgaatccac cgttttcgtt caaggcgtgg tcgagcggtt
     3181 tcaatccggc aaacgatctc tatggccgct tcgagtacgg catcccgccc gtcaaaaatg
     3241 gcgactacgc gtttctgctg cacatcctga agagcctgaa gagcagcggc aagggcgcgg
     3301 tgattctgcc gcacggcgtc ctgttccgcg gcaatgtcga ggccgacatc cgcacccgta
     3361 tcgtgaagca gggctacatc aagggcatca tcggcctgcc ggccaacctg ttttatggca
     3421 ccggcatccc tgcctgcatc gtggtgctgg acaaggagaa cgccgccgcc cgcaagggca
     3481 ttttcatgat cgacgccagc aagggcttca tcaaggacgg caacaagaac cgtctgcgcg
     3541 cccaggacat tcacaagatc gtcgacacct tcaccaagca gctcgaaatc gacaaatact
     3601 cgcgcctggt gccgctcgcc aagatcgagg ccgagggcta caacctcaac ctgccgcgtt
     3661 acatcgacag caccgaaccc gaggatttgc agagcatcga ggcgcatctc aatggcggca
     3721 ttcccgtggc ggacatcgaa gcgctccagt cgtattggga tatctgcccg aacctgagaa
     3781 ccgcgctctt taaaccaaac cgccccggtt acctcgacct ggtcgtggag aagggcgcga
     3841 tcaaacccgc catctacgaa caccccgagt tcaccgcctt caccgccggc atgaacacgc
     3901 acttcgatgt ttggcgcgcc aaggttgcaa aaaccctgaa agcgctcaag gcgggtttcc
     3961 gcccgaaaga aatgatcgtc agtttctccg agaacctgct tgctcactac aaagacaagc
     4021 cgctcatcga ccactacgac gtctaccagc acctcatgga ctactgggcc gaaaccatgc
     4081 aggacgactg ctatctggtc gccgccgagg gctggctcgt cggcaatcga gtccgggaga
     4141 tcattcaggt caagaacaag gacggaaagc tggtatggcc ggagtcgcac gactacaaga
     4201 gaggcaagag aaggttcaag tcggacctca tccccgccgc ggtcttgatc aaccgctatt
     4261 tcaagccgga acaggaaggc atcgacgtgc ttgaaagcgc cgttgccgcg ctggagcagc
     4321 agctcgatga acgccgagag gagcagggcg ccgaaggtgg cctgctcgaa gaagtcgtgg
     4381 agggtgaggg cgataagcag aagatcaccg ccaaggcggt aaaggcgcgt ctgaaagaaa
     4441 ttggcacgga tgcggccttc gccgacgaac aaacggcgtt gcgggaatat gccgatctgc
     4501 tcgaaaagct gacggagacc aagagccaac tgaagcaggc gcaggacgac ctggacgcca
     4561 agctgcacgc caagtacccg aagctcaccg aggccgagat caagacgctg gtggtggacg
     4621 acaaat
//
LOCUS       DBNGZE010000231         4535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_519288, whole genome shotgun sequence.
ACCESSION   DBNGZE010000231 DBNGZE010000000
VERSION     DBNGZE010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_519288"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16FV_06095"
     CDS             complement(<1..425)
                     /locus_tag="AC16FV_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR2849662.1"
                     /translation="MAPIFVRLSELGPIATAFHRLFLSLPALGLWLWIERRTQRVHRI
                     SLDRSTKRWLIAAGLFFAADLAVWHWSIKITTVANATLLANFAPIFVTLASWMFFGAR
                     FRVTFLMGMAVAFGGALVLMGDSLHLSRARLSGDLLGVLT"
     gene            complement(528..1745)
                     /locus_tag="AC16FV_06100"
     CDS             complement(528..1745)
                     /locus_tag="AC16FV_06100"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR2849663.1"
                     /translation="MAKIKKVVLAYSGGLDTSVILTWLRDTYDCEVVTFTADIGQGEE
                     LEPARAKAKKMGVKEIFIDDLKEEFVRDFVFPMFRANTLYEGEYLLGTSIARPRITKR
                     QIEIANKVGADAVSHGATGKGNDQVRFELGYYALRPDIRIIAPWREWDLTSREKLMTY
                     AEKRGIPVEMKRGKKSPYSMDANLLHISYEGGILEDPWKEPEESMWRWSVSPEKAPNR
                     PAYIELTYKKGDIVAINGKAMSPATVLATLNKLGGVNGIGRTDIVENRYVGMKSRGAY
                     ETPGGTIMLKAHRAIESITLDREVAHLKDDLMPRYASLIYNGYWWSPERKMLQAMIDT
                     SQETVNGVVRLKLYKGNVIVVGRKSDSDSLFDPAIATFEEDKGAYNKMDAEGFIKLNA
                     LRMRIAAKRRKKK"
     gene            complement(1849..3690)
                     /gene="lpdA"
                     /locus_tag="AC16FV_06105"
     CDS             complement(1849..3690)
                     /gene="lpdA"
                     /locus_tag="AC16FV_06105"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR2849664.1"
                     /translation="MPNMMEVKIPDIGDFTEVAVIEVMVKPGDTIKPEDPLITLESDK
                     ATMDVPSPAAGKVKDVKLKVGDKVAQGSLILLLETAEAAAPAPAKPAPKAEPAVPPRA
                     APAATPKFEVHAAEPAPTPRTGPLPGAARYTGKIDGECEVLVLGAGPGGYSAAFRAAD
                     LGKKTVLVERYATLGGVCLNVGCIPSKALLHVAAVTEEAQKLAEHGVSFGKPKFDLAK
                     LRDWKDKVVGKLVGGIGVMAKGRKVEVIRGSGRFIDPHHMEISLTAGEGKAPTSEKKI
                     IRFANAIIAAGSQAVKLPFIPEDPRIVDSTGALELRGVPKKMLVIGGGIIGLEMATVY
                     STLGCRLEVVEMLDGLMLGADRDLVRVWEKMNAHRFDRVMLKTKTTKVEALTTGIKVT
                     FEGEQAPDKPQVYDFVLVAAGRAPNGKNISAEKAGIAVSDRGFIEVDKQMRTNVPHIY
                     AIGDIIGQPMLAHKAVHEGHVAAEAAAGQKSIFDATVIPSVAYTDPEIAWVGMTEDEA
                     RKQGRAIGKAKFPWVASGRAIANGRDEGFTKIIVDEETHRVIGGGIVGTHAGDLISEL
                     ALAIEMGCDPEDIGKTIHPHPTLGESVGMVAELYEGVCTDLMPQKRR"
     gene            complement(3725..>4535)
                     /locus_tag="AC16FV_06110"
     CDS             complement(3725..>4535)
                     /locus_tag="AC16FV_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR2849665.1"
                     /translation="LDIIPWPKVDFSKFGSIETRPLSRIRKISGANLARNWVMIPHVT
                     QFDEADITGIERYRVVLNKAHEKDGIKVTVLAFLIKAAVEALKKFPEFNSSLDGENLV
                     IKHYYHIGFAADTPNGLVVPVIRNADRKSLLDLAREAAELAKKAREGKLQLAEMQGGC
                     FSISSLGGIGGTQFTPIINAPEVAILGVSRAEMKQKWDGKRFAPRLMLPLSLSYDHRV
                     IDGASGARFTTFFAQALASLVPAAGAPKRSTKPAKSRPAARKPTKKKSKKR"
BASE COUNT          808 a         1415 c         1389 g          923 t
ORIGIN      
        1 gtaagcacac ccagcaagtc accggataat cgcgcacggc tcaagtgcag gctgtcgccc
       61 atgagcacca gcgcaccacc gaatgctacc gccatgccca tgaggaaggt tacgcggaat
      121 cgcgcgccaa aaaacatcca acttgcgagc gtgacgaaaa tcggcgcgaa attcgcaagc
      181 agtgtggcgt tggcaacggt ggtgatcttg atggaccagt gccacaccgc cagatcggcg
      241 gcgaaaaata atccggcggc aatgagccag cgttttgtgc tccgatcgag ggaaatccga
      301 tgaacgcgtt gcgtacgtcg ctcgatccac aaccacaaac ccaacgctgg aagtgataaa
      361 aacagacgat ggaaagctgt ggctatcgga ccgagctcac tgagccggac gaagatcggc
      421 gccaacccga tggctgtggc accagtcaga agcgcgggga gcacaagacg gaaaggctgt
      481 tcaggatttg tgggcacgcc cttccaacca cgcagcgggg aaagggccta tttcttttta
      541 cgccgcttgg cagcgatgcg catgcgcaac gcattcagct tgatgaagcc ctcggcgtcc
      601 atcttgttgt aggcgccctt atcctcttcg aaggtcgcaa tcgccggatc gaacagactg
      661 tcggagtccg atttgcgccc gaccacaatc acgttgccct tgtagagctt gagtcgaacg
      721 acgccattca cggtttcctg cgaggtgtcg atcatggcct gaagcatttt gcgctcgggg
      781 ctccaccagt agccgttgta aatgagcgag gcgtaacgcg gcatcagatc gtccttcagg
      841 tgtgcgacct cgcgatccag cgtgatggat tcgatggcgc ggtgcgcctt gagcatgatc
      901 gtcccgcccg gcgtctcgta ggcgccgcgc gatttcatgc cgacgtagcg gttctcgacg
      961 atgtcggtgc ggccgatgcc gttgacgccg ccgagcttgt tgagcgtggc cagcaccgtc
     1021 gccggcgaca tggccttgcc gttgatggcg acgatatcgc ctttcttgta ggtcagctcg
     1081 atataggccg gcctgttcgg cgccttttca ggcgacaccg accagcgcca catggattct
     1141 tccggctcct tccacgggtc ctcgagaatg ccgccctcgt aggagatgtg caacaggttg
     1201 gcgtccatcg aatacggcga tttcttgccg cgcttcatct cgaccggaat gccgcgcttt
     1261 tcggcatagg tcatgagctt ttcgcgcgag gtcagatccc actcgcgcca cggcgcgatg
     1321 atgcggatgt cggggcgcaa cgcgtaataa ccgagctcga agcgcacctg gtcgttgccc
     1381 ttgccggtgg cgccgtgcga cacagcatcg gcgccgacct tgttggcgat ctcgatctgg
     1441 cgcttggtga tccgcggccg cgcgatggaa gtaccgagca ggtattcgcc ttcgtacagc
     1501 gtgttggcgc ggaacatggg gaacacgaag tcgcgcacga attcttcctt gaggtcgtca
     1561 atgaagattt ccttcacgcc catctttttc gccttggcgc gcgccggctc cagctcttcg
     1621 ccctggccga tgtcggcggt gaacgtcacc acctcgcagt cataggtatc gcgcagccag
     1681 gtgagaatga cggaagtatc gagaccaccg gaatacgcca gaacgacttt ctttattttt
     1741 gccatggttt agcgactgaa ataatcagca agaaagacgg cattgtagcg gattttccgg
     1801 cgtaccaccg ttacggcgga agccggatcg gcgccaccga catgcacgct accgccgctt
     1861 ctgcggcatc agatcggtac agaccccttc gtacaactcg gcaaccatgc cgaccgactc
     1921 gccgagcgtc ggatgcgggt ggatcgtctt gccgatatct tccgggtcgc agcccatttc
     1981 gatggcgagc gcgagctcgc tgatcaggtc accggcgtga gtacccacga taccgccgcc
     2041 gatgacgcga tgggtctctt catccacgat gatcttggtg aagccttcat cgcggccatt
     2101 ggcaatggcg cgaccggagg cgacccacgg gaacttggcc ttgccgatcg cccggccctg
     2161 tttcctggcc tcgtcctccg tcatgcccac ccaggcgatt tcggggtcgg tgtaggcaac
     2221 cgatgggatg accgtggcat cgaagatgga cttctgcccg gcggccgcct ccgccgccac
     2281 atggccctca tgtaccgcct tgtgcgccag catcggctgg ccgatgatat cgccgatcgc
     2341 atagatgtgc ggcacgttgg tgcgcatttg tttgtcgact tcgatgaaac cgcggtcgct
     2401 gacggcgata ccggccttct cggcggagat gttcttgccg ttcggtgcgc ggccggcggc
     2461 gaccagaacg aagtcataaa cctgcggctt atcgggcgcc tgctcgccct cgaacgtgac
     2521 cttgataccg gtcgtcaatg cctcgacctt ggtcgtcttg gtcttgagca tcacgcgatc
     2581 gaagcgatgc gcgttcatct tttcccacac gcgcacgaga tcgcggtcgg cgccgagcat
     2641 gagcccgtcg agcatttcga ctacctcgag gcggcaaccc agcgtcgaat acacagtcgc
     2701 catttcaagc ccgatgatgc cgccaccgat gacgagcatc ttcttcggca cgccccggag
     2761 ttcgagcgcg ccggtggaat cgacgatacg cgggtcttcg ggaatgaacg gaagcttcac
     2821 cgcctgggaa ccggcggcga tgatggcatt tgcaaagcgg atgatttttt tctcgctggt
     2881 cggcgccttg ccctcgcccg ccgtcagcga aatctccata tgatgcggat cgatgaagcg
     2941 cccgctgccg cgtatcacct cgaccttgcg acccttggcc atcacgccaa tgccgccgac
     3001 cagtttgccg accactttgt ctttccagtc gcgcagcttg gcgagatcga atttcggctt
     3061 gccgaaactg acgccgtgct cggcgagttt ctgtgcctcc tctgtcacgg ccgcgacatg
     3121 caacagtgcc ttggagggaa tgcagccgac gttgaggcac accccgccga gtgtcgcata
     3181 gcgctcgacg agcaccgtct tcttgccaag atcggcggca cgaaaggccg ccgagtagcc
     3241 gccgggaccg gcgccgagca ccagcacttc gcattcgccg tcgatcttgc cggtatacct
     3301 cgccgcgccc ggaagcggcc cggtacgtgg cgtcggtgcg ggttccgccg cgtgcacctc
     3361 gaacttcggt gtagctgctg gcgccgcccg gggtggcacg gccggctcgg ccttcggcgc
     3421 gggtttcgcg ggtgccgggg cagcggcttc cgccgtctcc agcagcagga tcagcgatcc
     3481 ctgcgcgacc ttgtcgccga ccttgagctt gacgtccttg accttgccgg cggccgggga
     3541 cgggacgtcc atggtcgcct tgtcggactc gagcgtgata agcgggtctt ccggcttgat
     3601 ggtatcgccg ggtttgacca tgacctcgat gacggcgact tccgtgaagt cgccgatgtc
     3661 ggggattttg acttccatca tgttaggcat ggcatccatt gccaccgcgc ggtggcctgg
     3721 tcagttagcg tttcttcgat ttcttcttcg tcggctttct tgcggcaggg cgcgactttg
     3781 ccggcttcgt gctgcgtttc ggcgctccag ccgctggcac cagcgacgcc agcgcctggg
     3841 cgaagaaggt ggtgaagcgc gcgcccgagg ccccgtcgat cacgcggtga tcgtaggaca
     3901 gcgacagcgg cagcatgagc cgcggcgcga agcgcttgcc gtcccatttc tgtttcatct
     3961 cggcgcgcga gacgccgaga atggccactt ccggcgcgtt gatgatcggc gtgaattgcg
     4021 tgccgccgat gccgccaagg ctcgagatcg agaaacaacc gccctgcatc tccgcaagct
     4081 ggagcttgcc ctcgcgcgcc tttttggcga gctcggccgc ctcgcgcgcg agatcgagca
     4141 gggatttccg atcggcgttg cggatcaccg gcaccacgag gccgttcggc gtgtcggcgg
     4201 caaaaccgat gtggtagtaa tgcttgatga ccaggttctc gccatcgagc gaactgttga
     4261 actcgggaaa cttcttgagc gcctccaccg cggccttgat gaggaacgcg agcacggtca
     4321 ccttgatgcc gtccttctca tgcgccttgt tgagcaccac gcggtaacgt tcgatcccgg
     4381 tgatgtccgc ctcgtcgaac tgcgtgacat gcgggatcat cacccagttg cgcgcgagat
     4441 tcgcgcccga gattttcctg atgcgcgaca gcggccgggt ctcgatcgag ccgaacttgc
     4501 tgaaatcgac cttcggccag ggaatgatgt cgagt
//
LOCUS       DBNGZE010000232         3508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1282309, whole genome shotgun sequence.
ACCESSION   DBNGZE010000232 DBNGZE010000000
VERSION     DBNGZE010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1282309"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="AC16FV_06115"
     CDS             complement(<1..410)
                     /locus_tag="AC16FV_06115"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR2849666.1"
                     /translation="MAVYAIGDVQGCYQPLQALLQKLRFDAACDCLWFTGDLVNRGPH
                     SLEILRFVRALGERAVCVLGNHDLHLLAVATGTAKTKKLDTLDGILHAPDRDELLYWL
                     RERPLLHHDADLGYTLIHAGLLPPWDLAQARRLAR"
     gene            complement(410..1378)
                     /gene="pip"
                     /locus_tag="AC16FV_06120"
     CDS             complement(410..1378)
                     /gene="pip"
                     /locus_tag="AC16FV_06120"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HIR2849667.1"
                     /translation="MHQLYPDIEPYATHRLRVDELHELHVEECGNVGGLPVLFVHGGP
                     GAGCEEAHRCFFDPNIYRIILFDQRGAGRSTPHASLDNNTTQDLVADMEKIRAHLGVD
                     RWVLFGGSWGSTLSLVYAETHPGRVLGLILRGIFLCRPWEIRWFYQEGANRVFPDHWR
                     EFIAPIPEPERHDLLHAHHRRLTGEDEVARMASAKAWSLWEGRAATLLPNKHVVEFFG
                     APHTALSLARIEAHYFVHDSFLKPEQILRNAPALSGIPGTIVHGRYDMVCPLQNAWEL
                     VQAWPDARLDIIPNAGHSAMEAGIINALVKATIDMAAQLGRPPQAS"
     gene            complement(1388..2203)
                     /locus_tag="AC16FV_06125"
     CDS             complement(1388..2203)
                     /locus_tag="AC16FV_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HIR2849668.1"
                     /translation="MVPKTNELSARLPANKPLPARACFLGERLNLRGLPEPAVTLGPL
                     TVPAGEAGMAVLFRYGAVVLFNLSEVEQKNFLTDLRPRVENPLRRMETEDVQLYVVGN
                     QPEGVAPEGIGIADLALARLQIVAIALARSVALAYYESAMASAFDLIEPLARRLENPR
                     GGGRRLRELLRHIGGTLLVLHKMTGRVEIQDKPDLLWDHPEVERLYLHLENEYELRER
                     NTVLERKLALINQTAETAVNLMQNRSMLRVEWYIVILIVFEVLLYTYEIWLKH"
     gene            complement(2222..3070)
                     /gene="metF"
                     /locus_tag="AC16FV_06130"
     CDS             complement(2222..3070)
                     /gene="metF"
                     /locus_tag="AC16FV_06130"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR2849669.1"
                     /translation="MPAQKKSQRTFSFEFFPPRNEDGRAKLRETVEQLAQLKPKFFSV
                     TFGAGGSTREGTYETVRNIQTMGQQAAPHISCIGTTRADIRALLDSYRKLGIRHLVAL
                     RGDLPKDAPKTGGDFHYANELVSFIRAETGDEFYLEVAAYPEFHPESPSPESDIRHFS
                     NKVRAGANSAMTQYFYNVDAYWRFVEEANALGVGVPIVPGIMPITNYKQLAKFSDACG
                     AEIPRWIRRRLEGFGEDLDSIRAFGLDVTTDLCDKLLEGGAPGLHFYTLNRAGPTCTV
                     WERLGL"
     gene            complement(3187..>3508)
                     /locus_tag="AC16FV_06135"
     CDS             complement(3187..>3508)
                     /locus_tag="AC16FV_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR2849670.1"
                     /translation="HVIFPDGKKIIVLAKGRLVNLGCATGHPSFVMSASFSNQTIAQM
                     ELFQNTSRYEKKVYVLPKKLDEKVARLHLKKIGAHLTPLSKTQADYIGVPVDGPYKPD
                     HYRY"
BASE COUNT          663 a         1061 c         1115 g          669 t
ORIGIN      
        1 cgcgccaacc gccgtgcttg cgcaagatcc cacggcggca acaggccggc gtgaatcaat
       61 gtgtaaccga gatcggcatc gtggtgcagc agagggcgct cgcgcagcca gtacagcaac
      121 tcatcacgat cgggcgcgtg caaaatcccg tccagcgtgt cgagcttttt tgtcttggcc
      181 gtaccggttg cgacggcgag cagatgcaga tcgtgattgc cgagcacgca cacggcgcgc
      241 tcgcccaatg ccctgacgaa gcgcaatatc tcgagcgagt gggggccgcg attgaccaga
      301 tcgccggtga accacaggca atcacacgcg gcatcgaaac ggagcttctg gagcagcgct
      361 tgcaggggtt gatagcagcc ctgcacatcg ccgatggcgt agaccgccat caggaggcct
      421 gcggcggtcg tcccagttgc gcggccatgt cgatcgtggc ctttaccagg gcgttaataa
      481 tgcctgcttc catggccgag tgccccgcgt tcggaatgat gtcgagccgg gcatcgggcc
      541 atgcctgcac cagttcccag gcgttctgca gcgggcagac catgtcatag cgtccgtgca
      601 cgatggtgcc ggggatgccg ctcagcgccg gggcgttgcg caaaatctgc tcaggcttga
      661 gaaaactgtc gtgcacgaaa taatgtgcct cgatgcgcgc cagcgacagc gccgtgtgcg
      721 gcgcgccgaa aaattccacc acatgcttgt tcggcaacag cgtcgccgcg cggccttccc
      781 acaatgacca ggccttggcg ctggccatgc gcgcgacctc gtcctcgccg gtcaggcgcc
      841 ggtgatgggc gtgcagcaga tcatggcgct ccggctccgg aatcggcgcg atgaattccc
      901 gccagtgatc gggaaacacg cggttagcgc cttcttggta gaaccagcgg atctcccagg
      961 gacggcacaa aaagataccg cgcaggatga gcccgagcac gcggccggga tgggtctcgg
     1021 catagaccag cgacagcgtc gagccccagg agccgccgaa caggacccag cgatccacgc
     1081 cgagatgcgc gcggatcttt tccatgtcgg cgaccaggtc ctgtgtggtg ttgttatcga
     1141 gactcgcgtg cggtgtcgag cgcccggccc cgcgctggtc gaacaggatg atacggtaaa
     1201 tgttgggatc gaaaaaacag cggtgcgctt cctcgcaacc ggcgccaggc ccgccgtgca
     1261 cgaacagcac gggcaggccc ccgacgttgc cgcattcctc gacatgcaac tcgtgcagct
     1321 cgtcgacccg gagccggtgc gtcgcatagg gttcgatgtc gggatagagt tgatgcacgg
     1381 cgttgcttca atgcttgagc cagatctcgt aggtgtacag caacacctcg aacacgatga
     1441 gaatcacgat gtaccattcc acgcgcagca tgctgcgatt ctgcatcaga ttcaccgcgg
     1501 tctcggcggt ctggttgatg agcgcgagct tgcgctcgag cacggtgttg cgttcacgca
     1561 gttcatattc gttttcgagg tgcagataca ggcgctccac ttccgggtgg tcccacaaca
     1621 ggtcgggctt gtcctggatc tcgacgcgtc cggtcatctt gtgcagcacc agcagcgtgc
     1681 cgccgatgtg acgcagcagc tcgcgcaacc ggcgcccgcc gccgcgtggg ttttccaggc
     1741 ggcgcgccag cggttcgatc aggtcgaagg cgctggccat ggcgctttcg taataggcca
     1801 gcgccacgct gcgcgccagc gcgatggcta cgatctgcag acgcgccagc gccaggtcgg
     1861 cgatgccgat gccctcgggg gccacgccct ctggctggtt gcccacgacg tatagctgaa
     1921 catcctcggt ttccatgcgg cgcagcggat tctccacgcg cggccgcaga tccgtgagaa
     1981 aattcttctg ctccacctcg ctcaggttga acagcaccac ggccccgtaa cggaacaaca
     2041 ccgccatccc cgcctctccc gctggcactg tcagtgggcc aagcgtgacc gcgggttcgg
     2101 gcagtccgcg cagattcagg cgctcgccca gaaaacaggc gcgcgccggc agcggcttgt
     2161 tggccggcag gcgtgcgctc agttcattgg ttttggggac catatcggtc tgtgatgaag
     2221 ttcagagtcc gagccgctcc cacacggtgc aggtcgggcc ggcgcggttg agggtataga
     2281 aatgcagccc cggcgccccg ccctcgagca acttatcgca caagtcagtg gtgacatcga
     2341 ggccgaaggc gcggatggaa tcgagatcct cgccgaagcc ttccagccgt cggcgaatcc
     2401 aacgcgggat ttcggcgccg caggcatcgg aaaatttcgc gagctgtttg taattggtga
     2461 tcggcatgat gcccggcaca atcggcacgc cgactcccag cgcgttcgct tcttcaacga
     2521 aacgccagta agcgtccacg ttgtaaaaat actgggtcat ggccgaattg gccccggcac
     2581 ggaccttgtt cgagaaatgg cggatatcgg actcggggct gggcgattcg gggtggaatt
     2641 ccgggtaggc cgccacctcc agataaaact catcgccggt ctcggcgcgg atgaagctca
     2701 ccagctcgtt ggcgtagtga aaatcgccgc cggttttggg cgcgtccttg ggtaggtcac
     2761 cacgcagcgc gacgagatgg cggatgccga gtttccgata ggaatcgagc agcgcccgga
     2821 tgtcggcgcg cgtggtgccg atgcaggaaa tatgcggcgc ggcctgctga cccatggtct
     2881 ggatgttcct gacggtttcg taagtgccct cgcgcgtgct gccaccggcg ccgaaggtca
     2941 cggagaaaaa cttgggtttg agctgtgcca gctgttccac ggtttcgcgc agtttggcgc
     3001 gcccatcctc gttgcgcggc gggaaaaact cgaagctgaa agtgcgctga gatttttttt
     3061 gtgcaggcat atatgagttt tcgccgcaaa gacgcgaaga acgcaaaggg aagcaaaatg
     3121 taaaatcagg ataactatcg ttcaatcccc gatttctttg cgtcctttgc gcctttgcgg
     3181 cagaaattaa taacggtaat ggtccggctt gtacgggccg tcgaccggca cgccgatgta
     3241 atcggcctgg gtcttggaca gcggtgtgag gtgggcgccg attttcttga gatgcaggcg
     3301 cgccactttc tcgtccagct tcttcggcag cacgtacacc ttcttctcgt accggctggt
     3361 gttctggaac agctccatct gcgcgatggt ctggttcgag aacgatgccg acatcacgaa
     3421 cgagggatgg ccggtggcgc agccgaggtt caccagtcgc cccttggcca gtacgatgat
     3481 tttcttgccg tcgggaaaga ttacgtga
//
LOCUS       DBNGZE010000233         4084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_519469, whole genome shotgun sequence.
ACCESSION   DBNGZE010000233 DBNGZE010000000
VERSION     DBNGZE010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_519469"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="AC16FV_06140"
     CDS             complement(<1..592)
                     /locus_tag="AC16FV_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR2849671.1"
                     /translation="MSLIRPFAGLRPVSRRAAEVIAPPYDVLSTEEARTRAQGRPWSF
                     LHISKPEIDLPVGTDPYSVAVYARGRENFDKILKEGVLGRDPEPCYYVYRLVMGAHHQ
                     TGIVAAASIHAYDANRIRKHEFTRPDKEDDRVRQIEALNAQTGPVFLTYRRSSAVDAI
                     IDKVTLGTPEVDITADDGVRHTQWVMRDPHQIDAITA"
     gene            734..1777
                     /locus_tag="AC16FV_06145"
     CDS             734..1777
                     /locus_tag="AC16FV_06145"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HIR2849672.1"
                     /translation="MADGCRNYLPPEWAPQSGVMLTWPHAYGDWAKRLKQVEPVFTEI
                     ARQVALREKVLISCYDRAHREHVGKLLAGVGVDMGRVILRTVPSNDTWARDHGPLTVM
                     CHGETMLLDFGFNGWGGKYGYELDNQISRKLYSMDAFGHAPMQTIDMVLEGGSIEVDG
                     SGTLLTTARCLLAPTRNPKLSKEQIEERLAAALGINRILWLQHGYLAGDDTDSHIDTL
                     ARFCDSRTIAYVRCDDPDDEHYAELKAMEAELKSFRAADGQPYRLVPLPWPRAKFDED
                     GNRLPATYANFLVINGAVLVPTYEDAADNEALARLKECFPDREAVAINCLPLIYQFGS
                     LHCVTMQLPEGVL"
     gene            complement(1866..2132)
                     /locus_tag="AC16FV_06150"
     CDS             complement(1866..2132)
                     /locus_tag="AC16FV_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849673.1"
                     /translation="MKRKLILLASLLLISPLAMAYQVTGPVLEVTDSKIVVQKGKEKW
                     ELARDAGTKVTGDVKVGDKVTVEYKMTATSIEVKGGGKAKGKSK"
     gene            2239..3126
                     /locus_tag="AC16FV_06155"
     CDS             2239..3126
                     /locus_tag="AC16FV_06155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HIR2849674.1"
                     /translation="MNSTLRVGLVQHACTEDRDRNLAASMAGIRDATARGAKLVLLQE
                     LHTGVYFCQTEDTKRFDQAEPIPGPTTQELGKIARELGVVIVGSLFERRAPGVHHNTA
                     VVLEADGSLKGQYRKMHIPNDPGYCEKFYFTPGDQGFAPIATSVGTLGVLVCWDQWYP
                     EAARLMAMHGAEILLYPTAIGWDPRDDEAEKTRQREAWIIIQRAHAIANGLPLVACNR
                     VGTEPDPSGRTPGIQFWGSSFIAGPQGEFLAQATTDKAEVLVAEVSRKRSEDVRRIWP
                     FFRDRRIDAYQDLLKRYGK"
     gene            3295..>4084
                     /locus_tag="AC16FV_06160"
     CDS             3295..>4084
                     /locus_tag="AC16FV_06160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR2849675.1"
                     /translation="MAKPILINSAAELLQRILSEKMPADRQMEGYFRAHRDMGVRDRG
                     FVAETVYGCLREKRWLEHVAGNALLLDVVAAWLLTHGYSARALEETGFRGDARGMVER
                     ARTFDKTTLPFAIQANMPDWLAQHLLAQFGEAEALALSAALNQPAPVDMRVNTLKAKR
                     DEVQTRLAQEGFPCEPTPWSPSGLRRHDRAPIFKTLCFKEGLFEVQDEGSQLLAPMLE
                     PKRHEMVVDFCAGAGGKTLHIGALMANTGTVYAFDVLAKRLEKLK"
BASE COUNT          811 a         1266 c         1233 g          774 t
ORIGIN      
        1 gcgccgtgat ggcgtctatc tgatgcggat cgcgcatgac ccactgcgta tgacgcacac
       61 catcgtcggc ggtgatgtcc acctccggcg ttccaagcgt gactttgtcg atgatcgcat
      121 ccaccgcgga tgagcgccga taggtcagaa ataccgggcc ggtctgggcg ttcagcgcct
      181 cgatctggcg cacgcggtcg tcctccttgt ccggccgcgt gaactcgtgc ttgcggatgc
      241 ggttggcgtc gtaggcatga atcgaggcgg ccgccacgat gccggtctgg tgatgcgcgc
      301 ccatcacgag acgatatacg taataacaag gctcaggatc gcgcccgagc acgccttctt
      361 tgagaatctt gtcgaaattt tccctgcccc tggcgtacac cgccacgctg tacggatcgg
      421 tgcccacggg cagatcgatc tcgggcttgg agatatgcag gaaactccag ggcctcccct
      481 gcgcgcgcgt gcgcgcctct tccgtgctga gcacatcgta aggcggcgcg atgacctccg
      541 cggcgcgacg tgacaccgga cgcaggccgg caaaaggacg aatcagggac atttcagctc
      601 cggggaagac aaaaaagagc gcacattcaa aaccacttgc cgacagaaat caaaccgccc
      661 gtgtgtgctt ggcgcggcgt catggctggg ctagcatacg cggcgttttt gaaccggacg
      721 cgataaaaac ccgatggccg acggctgccg caattatctt cctcccgagt gggcgcccca
      781 gagcggggtc atgctcacct ggccgcatgc gtacggcgac tgggccaagc gcctgaaaca
      841 ggtcgaaccg gtattcaccg agattgcgcg gcaagtagcc ctgcgcgaaa aggttctcat
      901 ctcctgctac gaccgcgcgc atcgcgaaca tgtcgggaag ctgctggccg gggtcggcgt
      961 ggacatgggg cgggtgattc tgcgcaccgt gccttccaac gatacctggg cgcgcgacca
     1021 cggaccactg acggtcatgt gccacggcga aaccatgctg ctcgatttcg ggtttaacgg
     1081 ctggggcggc aagtacggct acgagctgga caaccagatc agccgcaagc tgtacagcat
     1141 ggatgccttc ggccatgctc ccatgcaaac catcgatatg gttcttgagg gtggctcgat
     1201 cgaggttgat ggctccggca cgctgctcac cacggcgcgc tgcctgctcg cgcccacgcg
     1261 caacccaaaa ctctcgaagg agcagatcga ggagcgcctg gcggctgcgc tcggcatcaa
     1321 ccgcatcctg tggctgcaac atggttatct cgccggtgac gacaccgaca gccacatcga
     1381 cacgctcgcg cgtttctgcg acagccgcac gattgcctac gtgcgctgcg acgatccgga
     1441 cgatgagcat tacgcggaac tgaaggccat ggaagctgaa ttgaaatcct tccgtgccgc
     1501 ggacggccag ccctaccgcc tcgtgccact gccctggccg cgtgcgaaat tcgacgagga
     1561 cggcaatcgc ctgcccgcga cctatgccaa ctttcttgtc attaacgggg cggtgctggt
     1621 tccgacctat gaagatgcgg cggacaacga ggcgctggcg cgcctgaaag aatgtttccc
     1681 ggaccgcgag gcagttgcca tcaattgcct gccgctcatt taccagttcg gcagcctgca
     1741 ttgcgtgacc atgcagttgc cggagggcgt gctttagcta caggcaggat tgtcccggca
     1801 acgacccgaa caaaaacggg aaggccatga cgggccttcc cgtttttcaa gacagtaaac
     1861 gcgatttact tgctcttgcc cttggctttg cctccgccct tgacttcgat gctggtggcg
     1921 gtcatcttgt attcgaccgt caccttgtca cccactttga cgtcacccgt caccttggtg
     1981 ccggcgtcgc gggccagctc ccacttctcc ttgcccttct gcaccacgat cttgctgtcg
     2041 gtcacttcca gcaccgggcc ggtgacctga taggccatcg ccaacgggct gatcagcagc
     2101 aggctcgcga gaagaatcag tttgcgtttc atggatcaac tccttttgtt gtattgagga
     2161 agcaaaagat aggaaattgc cgccgccagt gtcaagcgtt gggaaaaatc cgcaacggaa
     2221 catgctaaca ttggcgccat gaactcaacg cttcgagtcg gtctggttca gcatgcctgc
     2281 actgaagacc gcgacagaaa tctggccgcc tcgatggcgg gcattcgtga tgccaccgcg
     2341 cgcggcgcca agctggtact gctgcaggaa ctccacaccg gggtttactt ctgccagacg
     2401 gaagacacca agcgcttcga ccaggccgag cccatacccg gcccaaccac gcaggagctg
     2461 ggaaagatcg ccagggaatt gggcgtcgtt atcgtcggct cgctgttcga acgccgcgcc
     2521 cccggggtgc atcacaacac ggcggtggtg ctggaggcgg acggcagcct gaaaggccag
     2581 taccgcaaga tgcatattcc gaatgacccg ggctactgcg aaaaatttta tttcacgccg
     2641 ggcgatcagg gtttcgcgcc catcgccact tcggtcggca cgctcggcgt gctggtctgt
     2701 tgggatcagt ggtatccgga agcggcgcgc ctgatggcga tgcacggcgc ggaaatcctg
     2761 ctctacccca ccgccatcgg ctgggacccg cgcgacgacg aggcggaaaa aacccgccag
     2821 cgcgaggcct ggatcatcat ccagcgcgcg cacgcgatcg ccaacggcct gcccctggtc
     2881 gcgtgtaacc gcgtcggcac cgaacccgat ccgtccgggc gcacgccggg catacaattc
     2941 tggggctcga gcttcatcgc cggaccacag ggcgaatttc tggcgcaggc aacaaccgac
     3001 aaggccgaag tcctggtggc ggaggtcagc cgcaaacgtt ccgaagacgt gcgccgcatc
     3061 tggcctttct ttcgcgaccg gcgcatcgac gcctaccagg atctcctcaa acggtatgga
     3121 aaataatttt gaaccgccaa gacgccaaga acgccaggag aatcaaaatt ataaagcctc
     3181 acgtatctgt ctttcgcgtg aatagcgatc atttctcgct gcttctttag cattattcag
     3241 cttacttgaa atttttttag acggctttct tggcgtcttg gcggttaact ttttatggct
     3301 aaaccaatcc tcatcaattc cgctgccgag ctgctgcaac gcattctcag cgaaaaaatg
     3361 cccgccgacc ggcagatgga gggctatttc cgggcacacc gggacatggg cgtgcgtgac
     3421 cgcggcttcg tggcggagac ggtctacggc tgtctgcgcg agaaacgctg gctggagcac
     3481 gtcgccggca atgccctcct gctggacgtg gtggcagcct ggctgctgac acacggctac
     3541 agcgcccgtg cgctcgaaga aaccggtttc cgcggcgatg cgcgcggaat ggtcgagcgt
     3601 gcacgcacgt tcgacaagac cacgctgccg ttcgcgatcc aggcgaacat gccggattgg
     3661 ctggcgcaac atctgttggc gcaattcggc gaggcagaag cgctggcatt gtccgcggcg
     3721 ctgaaccagc ccgcgccggt ggacatgcgc gtgaacacgc tcaaggccaa gcgcgatgaa
     3781 gtccagacgc gcttggcgca ggagggcttc ccgtgcgaac cgactccctg gtcgccttcc
     3841 ggtctgcgac gccatgaccg tgcgccgatc ttcaagacgc tttgtttcaa agagggacta
     3901 ttcgaggtgc aggatgaagg cagccagctg ctggcgccga tgctggaacc gaagcggcac
     3961 gaaatggtcg tggacttctg cgccggggcc ggcggcaaga ctttgcatat cggcgcgctc
     4021 atggccaata ccggcacggt gtacgccttc gacgtgctgg cgaaacgtct ggagaaactc
     4081 aagc
//
LOCUS       DBNGZE010000234         3900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1351791, whole genome shotgun sequence.
ACCESSION   DBNGZE010000234 DBNGZE010000000
VERSION     DBNGZE010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1351791"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16FV_06165"
     CDS             complement(<1..191)
                     /locus_tag="AC16FV_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849676.1"
                     /translation="MAKIGSETVGAAGEKSKMTLFKDDRAEDVGAIVVAALVIAYIVI
                     VTTGKSAPPAPAPAAPPAAT"
     gene            complement(210..1352)
                     /locus_tag="AC16FV_06170"
     CDS             complement(210..1352)
                     /locus_tag="AC16FV_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR2849677.1"
                     /translation="MTDTGFIRKLPSAIPGLALIVITMVVIRNWVEPWMADAVVFGQK
                     GWLVKVTHLNYILLGIIFGMLYRNVLFGGSMPAIFSDGFKLSRLMIKTGIILLGSLYT
                     VQAIVQLGAFAIFLIGGFVLLSIVMVLWLGKVFGIDRSMTGVLANALSICGVSAAVAT
                     SPAVEAKGAHVAYAIATILGFGILTMFISPFIGHALGLTDLQYGAWIGTGILNSGQVL
                     AAALAFNPKIEPGSAVSVAEIWNIMRVISIPFAVFIVTVWYWAGKEAAEAAQSKKGLG
                     TLLVEKFPVFVIGFAAMVLLTSMGAFGDVGLKGGPPPSDTIKMLRLWMTWIFGFGLVG
                     LGGYIDFKELSQAGGTPLKVGLIVGVTKYVLALIVVLMIKDYLVSI"
     gene            complement(1605..2696)
                     /gene="ygfZ"
                     /locus_tag="AC16FV_06175"
     CDS             complement(1605..2696)
                     /gene="ygfZ"
                     /locus_tag="AC16FV_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR2849678.1"
                     /translation="MRSRSAIDGNQAVNDAWKSFIQSQGAILEGERVQHFGKPVEERR
                     AAAQGNVLADLSDLSLIRARGADTQNFLNGQLSNDVRQLDAAHSQLASWCSPKGRMLV
                     IFRLLRRGDDTLLQLPSSLLEPTLKRLRMFVLRAKVTLEDVDAELVSFGIPGPDAERL
                     LRDVTGFAPGEINGSETRDEITIARLPGPHSRFEIIAPTDAATKLWNSLKAKAAPVGP
                     SAWAWLDIMAGIPSVHPETSEAFVPQMANLEIVGGVNFKKGCYPGQEIVARMQYLGRL
                     KQRMYRAHYAGDVLPRPGDSIFAPDFPGQSAGTVVSAQPAPDNGFDLLAVIQISSAEA
                     GELHLENESGPQLLLLPLPYSVTPITRAE"
     gene            2840..3226
                     /gene="sdhC"
                     /locus_tag="AC16FV_06180"
     CDS             2840..3226
                     /gene="sdhC"
                     /locus_tag="AC16FV_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR2849679.1"
                     /translation="MGKNKKRPVYLNLLKIRLPVGGILSILHRATGAFLVLLLPFALY
                     ILDRSLQDPAAFAEISKCLMSLEGRIALLIMVWAFAQHFMSGLRHLLLDIGIGDSKAT
                     ARLNAWLAFAAAGSVVILTGVCLWNA"
     gene            3214..3561
                     /gene="sdhD"
                     /locus_tag="AC16FV_06185"
     CDS             3214..3561
                     /gene="sdhD"
                     /locus_tag="AC16FV_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR2849680.1"
                     /translation="MERLRATGSARAGMGEWLWQRVTSIYMAGFVVYVIVHLSLSPLP
                     DQTAWKAWFALGYVRLAWAIFILSILAHAWIGMRSIYLDYLHPLWLRFSVSLLTALGL
                     LALGLWAARILLA"
     gene            3561..>3900
                     /locus_tag="AC16FV_06190"
     CDS             3561..>3900
                     /locus_tag="AC16FV_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR2849681.1"
                     /translation="MNIARRRFDCLVIGAGGGGLRAALQLAQGEAHVAVVSKVFPTRS
                     HTVAAQGGIAAPLANSGEDNWHWHMYDTVKGSDYLGDQDAIEYMCRSAAHLVIELEHY
                     GVPFSRLENGR"
BASE COUNT          776 a         1150 c         1152 g          822 t
ORIGIN      
        1 gttgctgccg gcggtgcggc cggtgctggg gctggcggag cagacttgcc cgtagtcact
       61 atcacaatgt aggcgatgac gagcgcggcc acgacgatgg caccgacgtc ctcggcgcgg
      121 tcgtccttga agagcgtcat tttgctcttc tcgccggcag cgccgacggt ttcacttcct
      181 atttttgcca tgttcgcttc tcctagtaat cagatggaga ccaggtagtc cttgatcatc
      241 aggaccacga tcagcgccaa cacgtatttg gtgacgccca cgatgagtcc gaccttgagc
      301 ggcgtgccgc cggcctggga gagttccttg aaatcgatat agccgcctag tcccacgagg
      361 ccaaaaccga agatccaggt catccacagg cgcagcatct tgatcgtgtc ggacggcggc
      421 ggtccgccct tgaggccgac atcgccgaag gcgcccatgc tggtcagcag caccatggcg
      481 gcgaagccga tgacgaacac cgggaacttc tccaccagca aagtaccgag acctttcttg
      541 ctctgcgccg cttccgccgc ttccttgccg gcccagtacc acacggtcac gatgaacacc
      601 gcgaacggga tcgagatgac gcgcatgata ttccagattt ccgccaccga taccgcggaa
      661 cccggctcga tcttggggtt gaaggccagc gccgccgcca gtacctgtcc cgagttgaga
      721 atacccgtgc cgatccaggc accgtattgc aggtcggtga gacccagcgc gtgaccgatg
      781 aaggggctga taaacatggt caggatgccg aagccgagga tcgtggcgat cgcataggct
      841 acgtgcgcgc ccttggcttc caccgccggc gaagtggcca ccgccgcgct gacgccgcaa
      901 atgctgagcg cattcgcaag cacgccggtc atggaacggt caatgccgaa taccttgccg
      961 agccacagca ccatcacgat gctcaggagc acgaaaccac cgatgaggaa gatggcgaag
     1021 gcacccagtt gcacgatggc ttgcacggtg tagagcgatc ccagcaggat gatgccggtc
     1081 ttgatcatga ggcgggagag cttgaaaccg tcgctgaata tcgccggcat cgaacctcca
     1141 aagagcacgt tgcggtacaa catgccaaag atgatgccta acaggatgta attgaggtgg
     1201 gtgactttga cgagccagcc tttttggcca aaaaccacgg catcggccat ccatggttca
     1261 acccagtttc ggatcacgac catcgtgatc acgatcagcg ccaggccagg aatggcgctt
     1321 ggcaattttc ggataaaacc tgtatcagtc atagtttgtg ttcctccata gaaccattct
     1381 tctgtcgata cgtcggtttg tttctactgc cagttgggtg tgaattgagc gggtgagagt
     1441 tatgccatcc ttgattgtct cctttggtca ctgggcctac tgctcgattg aagcgaagca
     1501 gtttattaga atctgcttat attgtgactt gtgcgaaagc attcaagcac aagcctagac
     1561 accaaacaag cgatgtcaac gaggtgacgg catgagttgg atgtttattc cgcgcgggtg
     1621 atgggcgtaa cagaataggg caggggaagt aacaaaagtt gcggaccgct ttcgttttcg
     1681 aggtgcagct cgccagcctc agcgctgctg atctggatca cagccagcag atcgaaaccg
     1741 ttatccggcg cgggttgagc tgagaccacc gttcccgccg actggccggg aaagtccggc
     1801 gcgaaaatgg aatcgcccgg tcgcggcaaa acatcgcctg cataatgcgc gcgatacatg
     1861 cgctgcttga gcctgccgag gtactgcatg cgcgccacga tttcctggcc gggataacag
     1921 cctttcttga aattcacgcc gcccacgatt tcaagattcg ccatctgcgg cacgaaggcc
     1981 tcgctggttt ccggatgcac gctgggaatg cccgccatga tgtcaagcca cgcccatgca
     2041 gacggtccaa ccggcgccgc cttggctttc aggctattcc ataatttagt ggcggcatcc
     2101 gttggcgcga tgatttcaaa gcgcgaatgc gggccgggaa gtcgcgcaat ggttatttca
     2161 tcgcgcgtct cgctaccgtt gatctcgccg ggcgcgaagc ccgtcacgtc gcgcaataat
     2221 ctttccgcat ccggccccgg tattccgaaa ctgaccaact cggcatccac gtcttcgagc
     2281 gtgaccttgg cgcgcagcac gaacatgcgc aggcgcttga gcgttggctc cagcagggag
     2341 gaaggtaatt gcagcagggt gtcgtcgcca cgccgaagca gtctgaagat gaccaacatt
     2401 cgccccttgg gactgcacca ggaagcaagc tggctgtgcg ccgcgtcgag ctggcgcaca
     2461 tcattgctca gctgaccgtt aagaaaattc tgcgtatccg cgccgcgcgc ccggatcagg
     2521 gataaatcgg aaaggtcagc cagcacgtta ccctgtgcgg ccgcgcggcg ttcttccacg
     2581 ggcttgccga aatgctgcac acgctcgcct tcaaggatcg ccccttggct ttgaatgaat
     2641 gatttccacg catcgttcac ggcttggttt ccatcgatgg ctgaccggga tcgcatgata
     2701 gcggaatctc ggctggcgcg aaaaacggct ggacgccaga aatagcggga ttaaactata
     2761 gccacgttgg gattcccttc ttctgggtgc ggctaatcgg catcaacggg acaggaaaag
     2821 acggatttct aaaaaactca tgggaaagaa caagaaacgt cccgtatacc tgaatctcct
     2881 caaaatccgc ctgccggtgg gcggtatcct gtccattctg caccgcgcca cgggcgcgtt
     2941 tctggtttta ttgctgccgt tcgctctcta tatattggat cgctcgctgc aagaccccgc
     3001 ggcctttgcc gaaatttcca agtgtcttat gagtctcgaa ggccggattg cgctccttat
     3061 tatggtatgg gccttcgccc agcatttcat gtccggcctg cgccatctgc tgctcgatat
     3121 cggcattggc gattccaagg ccacggcgcg tcttaatgcc tggctggcct tcgcggccgc
     3181 aggatcggtc gtgattctga cgggggtttg tctgtggaac gcctgagagc cacgggcagc
     3241 gcccgcgccg gcatggggga atggttgtgg cagcgcgtga cctcgatcta catggccggc
     3301 ttcgtggttt atgtcatcgt gcacctgagc ctgtcgccgt tgcctgacca aaccgcttgg
     3361 aaggcctggt tcgccctcgg ttacgtgcgc ctggcttggg cgattttcat cctgagcatc
     3421 ttggcacacg cctggatcgg catgcgcagc atttatctcg attatctcca tccgctgtgg
     3481 ctgcgcttca gcgtttcgct gctcacggcc ctcggcctgc tggcgctggg cctgtgggcg
     3541 gcgcggatcc tgctcgcatg atcaatatcg cgcgccggcg tttcgactgc ctggtcatcg
     3601 gcgccggggg tggcgggctg cgcgcagcgc tgcaactggc gcagggcgag gcgcatgtcg
     3661 cggtcgtgtc caaggttttt cccacgcgct cgcataccgt cgcggcgcag ggcggcatcg
     3721 cggcaccgct cgcgaattcg ggcgaagaca actggcattg gcacatgtac gacaccgtca
     3781 agggcagcga ctatctcggc gaccaggacg cgatcgaata catgtgccgt tcggcagcgc
     3841 atctggtgat cgaactcgag cattacggcg tgccgttctc tcgcctcgag aacggtcgca
//
LOCUS       DBNGZE010000235         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1491117, whole genome shotgun sequence.
ACCESSION   DBNGZE010000235 DBNGZE010000000
VERSION     DBNGZE010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1491117"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16FV_06195"
     CDS             complement(<1..303)
                     /locus_tag="AC16FV_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849682.1"
                     /translation="MKLLFSNSASVVLIGSAIAVFGSLIAAFGVYLGSLQDTKESQHL
                     LASVTGGDSYPMIIISSDGSFFIYVEGKYPLHEVQGDIVDVESMREVQAKGADFRNS"
     gene            complement(717..1934)
                     /locus_tag="AC16FV_06200"
     CDS             complement(717..1934)
                     /locus_tag="AC16FV_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HIR2849683.1"
                     /translation="MKSSYDIVIVGGGMVGATLACALGNSAFKIALLDRAPATPPPEK
                     DYDLRVSALTLASRALFENLGAWEGMTRRRVSPVREMRVWSGEGSGSIHFNAAEIGEP
                     ALAYIVENSVIQTALIERLHQFTNIHHLCPVEVSDITLADNGAVVTLKDGRSLSTKLL
                     VGADGADSEVRRAAGIETQSFNLHQKGIVATVTTEKPHEATARQRFLATGPLAFLPLD
                     EPHTCSIVWSADTARADQLLALDDAAFISELQRCFGESLGKIQTISPRAGFPLALSHA
                     RAYTAPHLALVGDAAHTVHPLAGQGVNLGFLDAATLAEVLLDAVAKQRDIGAHAVLRR
                     YERWRKGDNLAMVSVTGGFKYLFGNELPVVSQLRNLGLDLTNAATPIKNLIMRRASGL
                     EGDLPKLAKRVWH"
     gene            2365..>2733
                     /locus_tag="AC16FV_06205"
     CDS             2365..>2733
                     /locus_tag="AC16FV_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849684.1"
                     /translation="MRRHTVLILLPILLAGCMTAAEHRAAVKGEEAERLTVGKVQREI
                     RVGMSGAEVIQVLGSPNIVATDDERREVWTYDKVSTETVHSESRGGVAALILGVGESA
                     FGAVAPRYVAGAGARATTQRT"
BASE COUNT          618 a          809 c          784 g          522 t
ORIGIN      
        1 tgaattacga aagtcagcac cttttgcttg aacctcacgc attgactcta catctacaat
       61 atccccttgt acttcatgta aaggatattt gccctccaca taaataaaaa aacttccatc
      121 actagaaata ataatcatcg gataagaatc tcccccagtc accgatgcta atagatgttg
      181 agattccttc gtatcttgta atgaccccag ataaacgcca aatgcagcaa taagcgagcc
      241 gaacacagcg atcgcagaac ctatcagtac gactgatgca gaatttgaaa agagtaattt
      301 catctttttc tctaagtcag taggcaaagc agtattaacc gatcagacag accctgcaag
      361 atcagttcag tgaaactcag gcaagacaag gtgttttcag cccccgtatg gcgcgcccga
      421 gcatcgcagc cggaatcggg gttctcgcgc gcctcgtgtt tgagcccgaa gcgcgcagtt
      481 ggcgcccggc gagttggggc gcgcgccgat tccggcgaga agcgcagggg gtgttcgcgc
      541 catccggggg aatggttttg gtgacttttg ccgaaacaaa agtcacccag ggtgcggggc
      601 ggagcaaccc gcaattagct tttgaatcgc gggcgaagcc cgctcgacta acccccctct
      661 cctcaatcct ctcccgcgag gggagaggaa gaaactcaga atgcgcaacc caaaactcaa
      721 tgccaaactc gcttggcgag cttcggcaga tccccttcca atccactcgc ccttcgcata
      781 attaaattct tgatcggcgt cgcggcattg gtgagatcga gccccagatt ccgcagctga
      841 ctgacgaccg gcagctcatt accaaacaaa tatttgaacc cgcccgtgac ggaaaccatg
      901 gcgaggttgt cgcccttgcg ccagcgctcg tagcgccgca gcacggcgtg cgcgccgatg
      961 tctctctgct tggccacggc atccaacaac acctcggcca gcgtggcggc gtccagaaat
     1021 cccaggttca cgccctgccc ggcaagcgga tgcaccgtgt gcgcggcgtc gccgaccaag
     1081 gccaggtgcg gcgcggtata ggcccgggcg tgcgacagcg ccagtgggaa tcccgcacgc
     1141 ggactgatcg tctgaatttt tcctagggat tccccgaaac agcgctgcaa ctccgatata
     1201 aaagccgcat cgtcgagcgc caacaattga tcggcgcgcg ccgtgtccgc cgaccagacg
     1261 atggaacagg tatgcggctc atcaagcggc aggaaggcca gcggcccggt ggcgagaaag
     1321 cgctggcgcg ccgtggcttc atgcggcttt tcggtcgtga ccgtggccac gatgcccttc
     1381 tgatggagat tgaacgactg ggtttcgatg ccggccgcgc gccgcacctc ggaatccgcc
     1441 ccatcggcgc ccaccagcag cttcgtactc agactgcggc catccttcaa tgtcaccaca
     1501 gcaccgttgt ccgcgagcgt gatgtcgctg acctcgaccg gacagagatg atggatattg
     1561 gtgaactgat gcagccgctc gatcagcgcc gtctggatca cgctgttctc gacgatatat
     1621 gccagcgccg gctcgccgat ctcggcagcg ttgaaatgaa tcgaaccgct gccttcgccg
     1681 ctccacaccc gcatctcccg caccggagag acacgccgcc gggtcatgcc ctcccacgca
     1741 ccgaggtttt cgaacaaggc gcgcgaggcc agcgtgagag cgctgacgcg caggtcatag
     1801 tccttctctg gcggcggtgt cgcgggcgcg cggtcgagca gcgcgatctt gaacgccgaa
     1861 tttcccagcg cgcaggcgag cgtggcacca accatgccgc cgccaacgat cacgatgtca
     1921 taagaagatt tcataaaatt cgaataaaca tgattaacag gatttcacaa gattaacagg
     1981 attaaaacag ggccacgtta attctgacaa ttttgtctgg aacaaaattg gacgcgcttt
     2041 agcgcgcccg tcagggtgcg cgcccgggat ggcgcgcatc aaatcctgtc cattacgcca
     2101 agccagaccg aagactacgg cgatatcatg ttcggacgac atggtctgga ggaaccacct
     2161 ccacgatcgg gtcatgatcg tgctgtgatt cggccgacga atcaagcctc tcgcacggac
     2221 cgtcgacaaa cacgtagagg tgtcgtggag gtcctgagct tggcggcatg cgccaccttt
     2281 gatatatgtc aagacggcta agcatatgcg tgctatttta aataaaaaag tattcaagtg
     2341 cggtcggaga aaaggagcat ttccatgcgc agacatacgg tcctgatact gctgccgata
     2401 ctgctcgccg gctgcatgac ggctgccgag catcgcgcgg cggtgaaggg tgaggaagcg
     2461 gagcggctta ccgtcggcaa ggttcagcgc gagattcgcg ttggcatgag cggggcggag
     2521 gtgatccagg tgcttggctc gcctaacatc gtcgctaccg acgacgagcg gcgcgaggtg
     2581 tggacctacg acaaggtatc caccgaaacc gtccattcgg agagccgcgg tggcgttgcg
     2641 gcgctgattc tcggtgtcgg cgagagcgcg ttcggtgcag tcgcgccgcg ctacgtggca
     2701 ggcgccggcg cgagggcgac gacgcaacgg aca
//
LOCUS       DBNGZE010000236         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_866063, whole genome shotgun sequence.
ACCESSION   DBNGZE010000236 DBNGZE010000000
VERSION     DBNGZE010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_866063"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="AC16FV_06210"
     CDS             complement(<1..568)
                     /locus_tag="AC16FV_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2849685.1"
                     /translation="MPDIIGYLIYLVPLMAVLFFYMRRHKKKEQHHATQMKEAVEAGL
                     TEPASLHPAFDPRRCLGSGACVKACPEGAIGIIKGKGHLVKPAVCIGHGACVASCPHD
                     AITLVFGTAKRGMDIPQVNPNFETNVPGLYIAGELGGMGLIRKAVEQGRQAMESIRKK
                     KTGNAPLDVVVIGAGPAGSTASLGALEHK"
     gene            808..1704
                     /locus_tag="AC16FV_06215"
     CDS             808..1704
                     /locus_tag="AC16FV_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HIR2849686.1"
                     /translation="MSQQQAIIRTTQAVTPPSATRDASRRFWLWPAMVLLVVTGWAVR
                     NGHYYEAGDDFGYYLGVGGGVMMLALLLYSLRKHFRFMHNWGATKHWFRLHMFLGITG
                     PTLILFHSTFRTGSVNATVALSCMLLVAGSGVIGRVIYRKIHHGLYGRSATLQEVQTN
                     LGIIGGDVKSKFHFAPQLEARLKVFETSALAEHRSAWSRFWLFVTVGLRAGWTYYAVA
                     KELERLGRDHARKHAWDSIKLEKRLVAGKMAVRRYLDAVIDVARFSAYERLFSLWHIL
                     HVPFVFMLIISGIAHVIAVHMY"
BASE COUNT          345 a          549 c          540 g          403 t
ORIGIN      
        1 gtttatgctc gagcgcaccg agcgaggccg tactgcccgc cgggccggcg ccgataacga
       61 cgacatccag cggtgcatta cctgtcttct tcttgcgaat cgattccatc gcctgacgcc
      121 cctgctccac cgccttgcgg atcagcccca tgccacccaa ctcgccggca atgtagaggc
      181 ccggtacgtt ggtttcaaag ttcggattga cctgggggat atccatgccg cgcttggcgg
      241 tgccgaacac gagcgtaatg gcgtcatgcg gacaggaggc gacacaggcg ccgtggccga
      301 tacacacggc cggtttcacc agatgtccct tgcccttgat gatcccgatc gcgccctccg
      361 ggcacgcctt gacgcaggcg ccggaaccga gacagcgacg ggggtcgaac gccggatgca
      421 gtgaagccgg ctccgtcagt ccggcctcga cggcttcctt catttgcgtc gcgtggtgct
      481 gttccttttt tttatgccga cgcatataga aaaacagcac cgccatcagc ggaaccagat
      541 aaatcagata accaatgatg tcaggcatgg cttcacaaaa aattcacgat ttgacatgtc
      601 cgtttgtcag ccagtacacg cgcttcatgc ggcttatcgt ggtcttgaat ccgcggttac
      661 tctttaatta atcagttgca tgaaagtaat ggattttcgg gcttcggcat gcccatgaca
      721 agccgcgtag gcgggccgtc gtaagcaaga atagatcaaa aagatgatcc tgcccctgag
      781 gggcaggctt ggcagcaagg ggagacgatg agtcagcagc aggcaatcat tcgtaccacg
      841 caggccgtga cgccgccatc cgccacccgt gatgcgtcgc ggcgcttttg gctgtggccg
      901 gcgatggtat tgctggtcgt gaccggctgg gccgtcagga acggtcatta ttacgaggcc
      961 ggcgatgact tcggttatta ccttggcgtc ggcggcggcg tcatgatgct ggcgctgctg
     1021 ctctattcgt tgcgcaagca cttccgcttc atgcacaact ggggcgcgac caaacactgg
     1081 ttccgcctgc acatgtttct gggcatcacg gggcctaccc tgatcctgtt tcattccacg
     1141 ttccgcaccg gatccgtgaa cgcgaccgtg gcgctgagct gtatgctcct ggtcgccggc
     1201 agcggtgtca tcggccgcgt catctatcgc aagattcacc acggtttgta cggtcgcagc
     1261 gccaccctgc aggaggtaca gacgaatctc ggcatcatcg gcggcgacgt caaatccaaa
     1321 ttccacttcg ctccgcagct cgaagcccgg ctcaaggtat tcgaaacctc cgcgctcgcg
     1381 gagcaccgaa gtgcgtggtc acgcttctgg ctgtttgtca ccgtcgggtt gcgcgcgggc
     1441 tggacttatt atgcggtggc aaaagaactg gagcgtctcg gccgtgacca tgcccggaaa
     1501 cacgcttggg acagcatcaa actggagaaa cgtctggtcg ccggaaagat ggccgtccgc
     1561 cggtatctgg atgccgtcat cgacgtggca cgcttcagcg cctacgagcg tttgttctcc
     1621 ctgtggcaca ttctccatgt tccgtttgtg ttcatgctga tcatcagcgg gatcgcgcat
     1681 gtcatcgccg tgcacatgta ttgatctgac cgggaatggc tggcttggcg cggacatttt
     1741 tcacatggct ggtgatcggg cttttcagct tttgcctgcc gaccggctcc cgcgccgagt
     1801 cgctcgaaac cgtgctgatg ccggggaagg tcatcga
//
LOCUS       DBNGZE010000237         2329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1907936, whole genome shotgun sequence.
ACCESSION   DBNGZE010000237 DBNGZE010000000
VERSION     DBNGZE010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1907936"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC16FV_06220"
     CDS             <1..226
                     /locus_tag="AC16FV_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019589086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR2849687.1"
                     /translation="GKMLHIRARTREVFDVTGAGDTVIAAIGCAWAVGSGLEDAVQLA
                     NLAAGIVVGKPGAATASPAEIMQELGSKEG"
     gene            223..1236
                     /gene="rfaD"
                     /locus_tag="AC16FV_06225"
     CDS             223..1236
                     /gene="rfaD"
                     /locus_tag="AC16FV_06225"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HIR2849688.1"
                     /translation="MMYVVTGGAGFIGANLVKALNDRGEKDILVVDDLEHGDKFKNLV
                     DCEIADYLDKRDFLERINNGEFKKKFKAILHEGACSDTMEHNGVYMMVNNYEYSKVLL
                     YYCQTKRIPYLYASSAAVYGAGKVFRESHEHESPLNVYGYSKWQFDQYVRRLLPKKTA
                     QIVGFRYFNVYGDREQHKGRMASVAFHHFNQYRDSGKVKLFEGCDGYGNGEQRRDFIS
                     VEDVVAVNLFFLGQPQKSGIFNVGTGRAQPFNDVAAAVINGFRKLDGKPAASIKTLQD
                     EHQVEYIAFPEALKGKYQSFTQADVTALRRAGYKKAFLTVEEGVGRYISRLAEKEGIS
                     RKA"
     gene            1271..>2329
                     /locus_tag="AC16FV_06230"
     CDS             1271..>2329
                     /locus_tag="AC16FV_06230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR2849689.1"
                     /translation="MKKNDLENKTVAVVGLGYVGLPLAVEFGKRMATIGYDLNESKLA
                     SYRRCQDPTGGVGEDDLRAASKLKYSSKPTDMAPADFIIVAVPTPVDDAHQPDFSPLH
                     SACRTIAPHMKKGAIVIFESTVYPGATEEECIPVLEKGSGMRWMKDFHVGYSPERINP
                     GDKEHTLTTIKKVVSGDDTNTLETVAQLYELIVKAGVCRTSSIKAAEAAKVTENTQRD
                     LNIALMNELAIIFHKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHRIGVDPYYLTQNAD
                     RMGYHPEVILAGRRINDGMGKYVAEQTIKQIIQAGHSVNGSHVIVMGITFKEDCPDSR
                     NTRVIDVVNELKSYGVKVS"
BASE COUNT          548 a          606 c          691 g          484 t
ORIGIN      
        1 cgggaaaatg ctgcatatca gggcacgcac ccgggaagta ttcgatgtaa ccggcgccgg
       61 tgacacggtg attgccgcca tcggttgcgc ctgggctgtc ggcagcgggc tggaagacgc
      121 ggtgcagctg gccaacctgg ccgctggcat cgtcgttggc aaaccgggcg cagcgaccgc
      181 gagtccggcg gaaatcatgc aggaactggg atccaaggag ggatgatgta cgtcgtcacg
      241 ggtggtgccg gttttatcgg ggccaatctg gtcaaggccc tcaacgatcg tggtgaaaag
      301 gatattctcg tcgtggacga tctggagcac ggcgacaaat tcaagaatct ggtggattgc
      361 gaaatcgcgg actacctgga caagcgcgat tttctggagc gcatcaataa cggggagttc
      421 aagaaaaagt tcaaggccat cctgcacgaa ggcgcctgtt ccgacaccat ggagcacaac
      481 ggtgtttaca tgatggtgaa caattacgaa tattcaaagg ttctgctgta ttactgccag
      541 accaaacgca ttccttatct ctatgcttca tcggcggccg tttatggcgc cggcaaagtt
      601 tttcgtgaat cacacgagca tgagtcgccg ctcaacgtgt acggctactc caagtggcag
      661 ttcgaccagt acgtacgccg tctcctgcca aaaaagacgg cacagatcgt cggtttccgc
      721 tatttcaacg tctacggcga tcgcgagcag cataaggggc gcatggcctc ggtcgcgttc
      781 catcatttca accagtatcg cgattccggc aaggtcaagc tgttcgaagg ctgcgacggg
      841 tacggcaacg gcgagcagcg gcgggacttc atctccgtcg aggatgtcgt ggccgtgaac
      901 ctgtttttcc tgggccagcc gcaaaagagc ggcatattca acgtcggcac cggccgtgcc
      961 cagcctttca acgatgtcgc ggccgccgtg atcaacggtt tccggaaact ggacggcaag
     1021 cccgccgcga gcatcaagac ccttcaggat gaacatcagg ttgaatacat cgcttttccg
     1081 gaggcgttga aaggcaagta ccagagcttc acgcaagcgg acgtaacggc actgcgacgc
     1141 gcaggctaca agaaagcgtt tctgacggtg gaagagggag tcgggcggta catttcccgg
     1201 ttggcggaga aagaaggtat cagccgtaag gcgtgataaa aatccatttt taagtattga
     1261 gcatcaaggc atgaaaaaaa acgatttgga aaataaaaca gttgctgtcg tgggtctcgg
     1321 ttatgtgggt ttgccgctcg cggtggagtt cggaaaacgg atggcgacga tcgggtacga
     1381 cctgaatgag tccaaactcg ccagctatcg ccgttgtcag gacccgaccg gtggcgtggg
     1441 tgaggatgat ttgcgcgccg ccagcaagct caaatactcg agcaagccga cggatatggc
     1501 accggcggat tttattatcg tggctgttcc caccccggtg gacgatgcgc accaacctga
     1561 tttctcgcct ctgcactccg cttgccggac gatagcgccg cacatgaaga aaggagctat
     1621 cgtcatcttc gaatccacgg tttacccggg cgccacggag gaggagtgca ttccggtact
     1681 tgaaaagggt tcggggatgc gctggatgaa ggattttcat gtcggctact cgcccgaacg
     1741 catcaatccg ggcgacaagg aacatacact gacgaccatc aagaaagttg tttccggcga
     1801 tgacaccaac acgttggaaa cggtggcgca actttatgag cttatcgtca aggccggtgt
     1861 ctgccgcact tccagcatca aggcagctga agcggcaaag gtgaccgaga acacccagcg
     1921 cgatctgaac atcgcgctga tgaacgaact cgctattatt tttcacaagc tgggtatcga
     1981 cacgctcgag gtgctcgaag cggccggtac caagtggaat ttcctgccgt tccggccggg
     2041 actggtcggt ggccatcgca ttggtgttga tccttattat ctgacccaga acgctgatcg
     2101 catgggttac cacccggaag tgattctggc cggacgccgt atcaatgacg gcatgggaaa
     2161 atatgtggcg gagcagacca tcaagcagat tattcaggcg ggccattctg ttaacggcag
     2221 ccacgtgatc gtcatgggca ttaccttcaa ggaagattgc cccgattccc gcaatactcg
     2281 ggtgatcgat gtcgtcaatg aactcaagtc gtatggcgtg aaagtctcg
//
LOCUS       DBNGZE010000238         3280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_656755, whole genome shotgun sequence.
ACCESSION   DBNGZE010000238 DBNGZE010000000
VERSION     DBNGZE010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_656755"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1431
                     /locus_tag="AC16FV_06235"
     CDS             121..1431
                     /locus_tag="AC16FV_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR2849690.1"
                     /translation="MNKRVSKRVLILAWAALAAGSALAADPTTIDWTKVPGKTVTLFY
                     PGQSSYEWLRNDHKDEEGRKGVQRGQACDRCHDGEEKTLGDALVKSGSLEPTPVKGKD
                     GYKDLTVQAAFDDKNAYLRFQWKTLNNFPGSEHQYLRFDGKEWKVYGYPKLDKVVQEG
                     KQPGIYEDRMSVMIDDGKVPGFAQQGCWLTCHDGQRDMAKQFTKEEVAANPMLTAIKK
                     NDVRKYLPDTRTNPSDWKTGKSVEEIAKIKAAGGFVDLIQWRAHRSYPVGMADDGYVL
                     EFRNSDTGKDMFSGNADKETHLPKFMWDEKKVGYKSITAEQLRKGDHFLIREKNAVPF
                     DPKAGWKEGDMIPDYLVSREDAKGSAADNNAIASWKDGKWTVVIVRPLGLANDDDKAL
                     KAGGVYHVGFAVHDDNITTRGHFVSFVRTLGLGAKADIQAVKLP"
     gene            1904..2890
                     /locus_tag="AC16FV_06240"
     CDS             1904..2890
                     /locus_tag="AC16FV_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HIR2849691.1"
                     /translation="MKFLRQVVVLCALIGGLGGISIALAVDLPTLSEGKEKSAALGAK
                     DLFLKGDAKCTRCHDETDSPKPLAIGKTKHGTTADSRTPTCTTCHGDSDTHRVDVAEG
                     QVQPKPDRIFSKKSTTPAKELSETCLGCHEGSKRIHWQGSTHDARDVSCASCHTIHAS
                     HDKVRDKLTQAEVCFTCHKEQRAQMSRPSRHPIKEGKVACSDCHNPHGTAGPKLLVRD
                     NVNDTCYQCHMEKRGPFVRTHEPVQEDCSICHNPHGTTNPNLLKVRPPFLCQSCHEPT
                     SHHSTVPASGLVGGGNPSTNQAVIMARGCVNCHTNIHGSNNAADGSGRGFRR"
     gene            2924..>3280
                     /locus_tag="AC16FV_06245"
     CDS             2924..>3280
                     /locus_tag="AC16FV_06245"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HIR2849692.1"
                     /translation="MNAIRKYFLRLNALTAAVFATFGTAVAAEVDEVTELTKPESTVS
                     AGIGHVSKDNQRFGQYTGLNEEGAYGLLDVDFMQRDEQTGTWLKLNGRNLGFDNRELR
                     FEHNRQGDWGYFIDLSQ"
BASE COUNT          772 a          961 c          976 g          571 t
ORIGIN      
        1 acccccgcgg ccgcgtcttg gtttgcaacc ttgatcggcc gctgcgaccg tccatgcgat
       61 cctgattaaa actgtgacga gaaaatcgtg acgaaaagcc gattcaccaa ggagatcatc
      121 atgaacaaga gagtgagcaa gagagtattg atcctggctt gggccgcctt ggccgcggga
      181 tccgccctgg ccgccgatcc gaccacgatc gactggacga aagtccccgg caagacggtg
      241 acgctgttct atcccggcca gtcgtcatat gagtggctgc gtaacgatca caaggacgag
      301 gaaggcagaa aaggggttca gcgcgggcaa gcctgcgacc gatgtcatga tggtgaggaa
      361 aagacgctcg gcgatgcgct cgtcaaaagt ggttcccttg agccgacgcc cgttaagggc
      421 aaggacggct acaaggatct cacagtgcag gccgcgttcg acgacaagaa tgcctacctg
      481 cgcttccagt ggaagaccct caacaacttc ccgggcagcg agcatcagta cctgcgtttc
      541 gacggcaagg agtggaaagt ctacggctat cccaagctcg acaaggtggt gcaggaaggc
      601 aagcagcctg gcatctacga agaccgcatg tcggtcatga tcgacgacgg caaggtgccg
      661 ggttttgcgc agcagggttg ctggctcacc tgtcatgatg gccagcgtga catggcgaag
      721 cagttcacca aggaggaggt cgccgccaac ccgatgctca cggcgatcaa gaagaacgac
      781 gttcgcaagt acctgccgga cacgcgcacc aacccgtccg actggaagac cggcaaatcg
      841 gtcgaggaga tcgccaagat caaggccgcc ggcggtttcg tcgacctgat ccagtggcgc
      901 gcccatcgca gttatccggt tggcatggcc gacgatggct atgtcctcga attccgcaac
      961 agcgacacgg gcaaggacat gttcagcggc aatgccgaca aggaaaccca cttgccgaag
     1021 ttcatgtggg acgagaaaaa ggtcggctac aagtccatca ccgccgaaca gttgcgcaag
     1081 ggcgaccact tcctgatccg tgaaaagaac gccgtgccgt tcgatcccaa agcgggctgg
     1141 aaggaaggcg acatgattcc ggattacctc gtcagccgcg aggatgccaa gggttcagcc
     1201 gccgacaaca acgctatcgc cagttggaag gatggcaagt ggactgtcgt gatcgttcgc
     1261 cccctcggct tggccaacga cgatgacaag gccctcaagg cgggtggtgt ctaccacgtc
     1321 ggcttcgcgg tgcatgacga caacatcacc acgcgcgggc acttcgtctc gttcgtcaga
     1381 acgctggggc taggggccaa ggcggacatc caggccgtca agcttccctg atatttacgc
     1441 gcccattcgc gtcaccgtgc tggcggtggc atcgtgcgtc cggaacaggc gagagatccg
     1501 cctggtgccg aagggaaacg aacgcgtccc gggttatttc gagtcgctcg cgcatttcag
     1561 gcgagattca gttgactgaa ctgtcaggga atagcgcacg aactgcccag ggctatgagt
     1621 agaaaagacc gggcttgact gcccggagct cgaacggggt ccgaccccct tccccgtggc
     1681 cacgttttga tttcaaccac gggcggcccg ggctcgggct gcttcaagaa ttgatcttgg
     1741 tcaaattccc aaatgttgac cctgatcaaa actccaattg cgatatatca attacattaa
     1801 gcgcggtcgt gtgggagtat gcgaccaaga ttggcggacg gagtggttac tttttgtggc
     1861 cacgctaacg gcgaagatga aagaggatga tagggggtta acaatgaaat tcctgcggca
     1921 agtggtagtc ttgtgcgcac tcatcggtgg cctcggaggg atctctatcg cgctggcggt
     1981 ggatcttcca acactgagtg aaggaaaaga aaaatccgcc gcgttgggag ccaaggatct
     2041 ttttctcaag ggcgatgcca agtgtacgcg ttgccatgat gaaaccgaca gccccaaacc
     2101 actcgcgata ggcaagacca agcacggcac cacggcagac agtcgcacgc cgacttgcac
     2161 aacctgccac ggtgacagcg atacccatag agtggatgtc gccgagggcc aggtacagcc
     2221 gaaacccgac cgcatattct ccaaaaaatc gaccaccccc gccaaggaac tcagcgagac
     2281 gtgcctcggc tgccatgagg gcagcaagcg tattcactgg cagggcagca cacacgatgc
     2341 gcgcgacgtg agctgcgcct cctgtcacac aatccatgca tctcacgaca aggtgcgcga
     2401 caagctcacg caggccgagg tgtgcttcac ctgccacaag gaacagcgcg cccagatgag
     2461 ccggccgtcg cgccacccga tcaaggaagg caaggtcgcc tgctcggatt gccacaaccc
     2521 gcacggcacc gccggaccca aactgttggt gcgcgacaac gtcaatgaca cctgctacca
     2581 gtgccacatg gagaaacgcg ggccgttcgt gcgcacgcac gagccggtgc aggaggactg
     2641 ttcgatctgc cacaacccgc acggcacgac gaatcccaat ctgctcaagg tgcgcccccc
     2701 cttcctctgc cagtcgtgcc acgagccgac ctcgcatcat tcaaccgttc cggcaagcgg
     2761 cttggtgggt ggcggaaacc cgtccacgaa tcaggccgtg atcatggcgc gcggctgtgt
     2821 gaactgccac accaacatcc acggcagcaa caacgcggcc gatggaagtg gccgcgggtt
     2881 ccgccgttaa gcgttaacga cagctagcta gagagaggac gacatgaacg cgattagaaa
     2941 gtactttctc cgattgaacg cacttactgc tgcggtgttc gccacttttg gtaccgctgt
     3001 cgctgcggaa gttgacgagg tcaccgagct taccaagccc gaaagcacgg tcagtgcggg
     3061 aatcgggcac gtgtccaagg acaatcagcg cttcggccag tacaccggat tgaacgagga
     3121 gggtgcctat ggactgctcg atgtagattt catgcaacgc gacgaacaga ccggcacatg
     3181 gctcaagctc aacggacgca acctgggctt cgacaatcgc gagttacgct tcgagcataa
     3241 ccgccagggt gactggggtt atttcatcga cttgagccag
//
LOCUS       DBNGZE010000239         1613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1144373, whole genome shotgun sequence.
ACCESSION   DBNGZE010000239 DBNGZE010000000
VERSION     DBNGZE010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1144373"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16FV_06250"
     CDS             complement(<1..344)
                     /locus_tag="AC16FV_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR2849693.1"
                     /translation="MSKSAEQIDTLLKREYKAGFITDVEADSVPPGLNEDIIRLISAK
                     KNEPEFMREWRLKAYRHWLAMVTPAWAHVHYPPIDYQTIVYYSAPKAGKDGPKSLDEV
                     DPKLLETYEKLG"
     gene            complement(657..1199)
                     /gene="hemG"
                     /locus_tag="AC16FV_06255"
     CDS             complement(657..1199)
                     /gene="hemG"
                     /locus_tag="AC16FV_06255"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005553154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HIR2849694.1"
                     /translation="MPRTLILYATTEGHTAKIVQRIAQRLQAIRHVVETHHADAVPSG
                     LNPLAYDGIIVGASIHYGHHPRYLRVLVQRYRMALTSRPSAFFSVSLSAGGPGARPGT
                     AQRYLETFLRQVDWYPPQAATFAGALQYSKYGTFKRILMRMIVGMAGGDTDATRDYEY
                     TDWETVDRFADAFAQRFKQA"
     gene            complement(1275..>1613)
                     /locus_tag="AC16FV_06260"
     CDS             complement(1275..>1613)
                     /locus_tag="AC16FV_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR2849695.1"
                     /translation="GWLKSVYAGAARVVEALGGKVNPPPEGGRFPWDADKLIYVVREP
                     WPSKATGANLVYGVITKGKPLVVTSEMAENGTIFSDGIEADYLAFNAGLAATIRVADK
                     KANLVARSAS"
BASE COUNT          332 a          455 c          462 g          364 t
ORIGIN      
        1 atgccgagct tctcgtaggt ttcgagcagc ttggggtcga cctcatcgag actcttgggc
       61 ccatcctttc cggccttggg cgcggagtaa tagacgatgg tctggtaatc gatcggcggg
      121 taatgcacat gggcccaggc cggcgtgacc atggccaacc agtgccggta ggccttgagc
      181 cgccactcgc gcatgaattc cggttcgttt ttcttggccg aaatcaggcg aataatgtct
      241 tcattcaggc cgggcggaac cgaatccgcc tcgacgtccg tgatgaaacc cgccttgtat
      301 tcccgcttga gcagggtgtc gatctgttcc gctgatttgg acattgctac ctccaaatgc
      361 ccgcggctaa cgcagtttga aaatacccgc gctccgtgta agtaataaca tactggaata
      421 gtcaggtata aaaatactat tgttgatata ttcagtcaac tattatctac tcagatagcc
      481 ctggtaaacc caaggtttag cgccatcgca acacagcgct ttagatacac gcaatggcgt
      541 ggcacggatt cccgtgaatc tgagttgtag tggcccggcg taaaactggc gattttttcc
      601 ggatcttgcg gaatcagttt cgtgaaaaca cttcatccgc tcgcaaaaga catcgcttac
      661 gcttgtttga aacgctgtgc aaatgcatcc gcgaagcgat cgaccgtttc ccagtccgtg
      721 tattcataat cccgcgtggc atcggtatcc ccgccggcca tgccgacaat catgcgcatc
      781 aatattcgtt tgaacgtccc atacttgctg tactgcaatg ccccggcgaa ggtcgcggct
      841 tgcgggggat accagtcgac ttggcgcaaa aaagtttcga ggtagcgctg cgcggttccg
      901 ggtctggcgc ccggcccacc ggcgctgaga ctgaccgaga agaaagcgct gggccgggat
      961 gtcagggcca tgcggtaacg ctgtacaagc acgcgcaaat aacgcgggtg atggccgtag
     1021 tggatcgatg caccgacgat gattccgtca taggccaacg gattcaggcc ggacggaacc
     1081 gcatccgcat ggtgcgtttc gacgacatgc ctgatggctt ggagccgctg cgcgatgcgc
     1141 tgcacgatct tcgccgtgtg accttcggtg gtggcataaa ggatcagagt acggggcatc
     1201 ggacctgcct ttctgaccta actgtgcatg aaaccatagt ttggccgtta gtggaaagca
     1261 tggtttggcc attctcacga ggcgcttcgc gcgaccaggt ttgccttctt gtcggcgacg
     1321 cggatggtgg ctgcgagacc agcgttgaat gctaagtaat cggcctcgat cccgtcgctg
     1381 aaaatggtgc cgttctccgc catttcggag gtaaccacca gcggctttcc cttcgtgatc
     1441 acgccgtaga ccaggttcgc cccggtggcc ttgcttggcc agggctcgcg caccacgtaa
     1501 atgagcttgt cggcatccca ggggaagcgt ccgccttcag gtggcggatt gaccttgccg
     1561 cccaaggcct cgaccacacg tgcggcaccg gcgtagaccg atttgagcca acc
//
LOCUS       DBNGZE010000240         4483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1765867, whole genome shotgun sequence.
ACCESSION   DBNGZE010000240 DBNGZE010000000
VERSION     DBNGZE010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1765867"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="AC16FV_06265"
     CDS             complement(<1..468)
                     /locus_tag="AC16FV_06265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HIR2849696.1"
                     /translation="MTKIGAETKIAGMSAPQQSFRLLRLFLPKKLQPWLRGLRKRWKL
                     RSIKLDEPFRSVFPYVGASLPRQENLIRLSEYIEQRGIEGAVVECGVLDGGTAALMGY
                     ATKQSGRPIHLFDAWEGLPETTENDGEASKKWVGQVVGSPKRVAEIMSKLGISS"
     gene            956..1468
                     /locus_tag="AC16FV_06270"
     CDS             956..1468
                     /locus_tag="AC16FV_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849697.1"
                     /translation="MLYAVLQLKLKITFAQPTDLKRVAEPINRTNQFNLEGSRTSFKE
                     VAQWHASPRYVIVTAQTADRFGDMGTTCVAVARIDGEEMILLPFVLSCRVFGYGIERS
                     VLNYLKTLAADNGVRRIVGRYVPTPQNTPCRNFLPDNGFREEKERWVFDIGTPSPADA
                     EWLQVEVSVV"
     gene            1664..>4483
                     /locus_tag="AC16FV_06275"
     CDS             1664..>4483
                     /locus_tag="AC16FV_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HIR2849698.1"
                     /translation="MINQTKIWLACILAVGLIPVGTAHADTSDDTIRRPAVFRVTTDI
                     VNRNVLPFGALIGSGFGNTLIREGAGFEPVVFRNNFVALEDSPNRIVVAPDAMTYWDT
                     LREGFLDQATVHIYRIENGRFRMAREDRVTSGGAHASGWFRALANNQVVTPGVTRFRF
                     RWDDWNRPRARYFFTVRAIDKYGGLSPPASAFEIESPERLNKAPAAQNALVTFNPVKA
                     LFSNPTPPPAPGNFRGKLDSDGILTLEWEPVDSPDVAGYVVYRSDYPPQRHSGYYLQL
                     AGTPTSAEQNIKAGDMVIVSKKIYSPSRNRDISNRVWGASNEYDKLMPGLIHFFPDET
                     PGKTWSLVPHAANTPVDEPGETCLKLQLAADASESLSIYNHSGTGQFWYDVLEKKSYT
                     VEVWMRQEGSGTVQFKLGGFYKDAPQRIEPITFNVGPVWKKYVAHFTPTVIQSGARPN
                     DMVLKFTGPATFYVDNFRIYRTDTAYLEMLPHEYSAIKSSGILSLRTSGLIMTRTHTY
                     NMEQLTNSGGVINGTQKLNTLPQTLKIMRKAGVRPWLQIEFHMSPQEWLGFVEYMAAP
                     YDPKVDTPASKPWAYKRYRQGQARPWVEEFDQIYFELSNETWNRIFQPWIFNPMTDAV
                     SKKRYSPGQVYGLFQEYVHSIMRSSPYWRPADLDRKFDFILGGWESVMGYSRDAASVS
                     PSSDFLTIAPYIGGSDAAEGPPKPGAASLFNVLAQVNQSAIPVADLYAKELLDLNSKG
                     ARKLRLGTYEGGPGYVMNGLNNARVTEEEAHAQEQVMKSMAAGTATLDSFLARAYRGF
                     AVQNFSAFDSGDRWKSHAKWYHGGQAYPSWKLLALFNNEAGGDDMLRTETLSVPTAEL
                     RAFSRRQTIKDAPLAAIYATRKEDRYNLFVISRKVPGYPVVGDDGYTPVTVELPFSKA
                     KSITLYRMTGAPDANNLMGDNVRIEK"
BASE COUNT         1029 a         1342 c         1183 g          929 t
ORIGIN      
        1 ggaagaaatg cctagcttgg acatgatttc agcgacccgc tttgggcttc ctacgacttg
       61 gccaacccat ttcttgcttg cctccccgtc gttttccgtg gtttccggca aaccttccca
      121 cgcatcaaac agatggattg gacgaccgct ctgtttcgtg gcatatccca tcaatgccgc
      181 cgtccctcca tcaagtaccc cacactccac tactgcacct tctattccgc gctgttcgat
      241 atattcagat agacgaatta aattctcctg tctgggcaaa ctggctccca catatggaaa
      301 aacacttcga aaaggctcat ccagttttat tgaccgaagc ttccaccgct tccttaaacc
      361 tctcaaccag ggttgtagtt tcttcggcag aaacaacctc agcaacctga aagattgctg
      421 cggagcactc attcccgcta tttttgtttc agcaccaatc ttagtcatac acacctcatg
      481 gtcatcttgg taagactctg caattaatat aaacaacagt cagtagagct gacgaattgc
      541 gtttttatca gccatcatgg taatgcgttg tttgtttatt tgccacccaa atgtcattca
      601 gctgcctgtg gaagttcgct ctgccgaaaa acggtcattg gagtctgccg acatgttacc
      661 gcgtgacgaa gcgcacaccc cctcctgcct ctgccgggcg tcttgatata cgtgaccgat
      721 ggatgtttgt gagctctggc gagctggtac tatactgtcc gatccccaat gtgcgtggcc
      781 gaaaggctgg cttcatgacg gccttcccgc acagtgcgac cttgtccgct tgagaggccc
      841 aacatgacaa attcgaccga ccacggacac gcataacact gtggaaggaa tttctcgaag
      901 atgacctcga aatggatcgc accttacata ccaacagtgt gagaaggaaa agaccgtgct
      961 gtacgccgtg ttgcagttga agctgaagat cacgtttgcc cagcccaccg acctgaagcg
     1021 tgttgcagaa ccgatcaacc gaacgaacca gttcaacctg gaaggcagcc gtaccagctt
     1081 caaggaggtt gcacaatggc acgcctcacc ccggtacgtc atcgtgacgg cccaaacggc
     1141 cgatcgtttc ggtgacatgg ggacaacctg cgtcgccgtc gcgcgcattg atggcgagga
     1201 gatgatcctg ctgccattcg tgctgagctg tcgtgtcttc ggttatggga tcgagcgcag
     1261 cgtgctgaat tacctgaaaa cactggcggc agacaatggc gtgcggcgaa tcgtgggccg
     1321 ctacgtgcca acgccgcaga acaccccttg cagaaatttt cttcccgaca acgggttccg
     1381 tgaggaaaag gagcggtggg tgttcgacat cggcacgccc tcaccggccg acgccgagtg
     1441 gctgcaggtg gaagtgtcgg tggtatgatg gtgcaggtcc cggcgttgtg ccacgaccat
     1501 aaccaagatc acgatgaaac tatacgggct gatgtagcca cgaaggaaag actggcttca
     1561 atcctgttgc gcccaatcaa acaggaaaac acattcccat gatcagcctt tcactgccta
     1621 tcggtaccat caagatcgat ctctcacggc atggagattg tctgtgatta atcagacgaa
     1681 gatctggctg gcatgcattc ttgccgtcgg cctgattccc gtcggcaccg ctcacgcgga
     1741 tacgtccgac gataccatcc ggagacccgc agtcttcaga gtcacaacgg acatcgtcaa
     1801 ccggaacgtg ctcccgttcg gcgctctcat cggcagtggt ttcggcaata cactgattcg
     1861 cgagggggcc ggcttcgaac ccgtcgtctt ccgcaacaat ttcgtcgcgc tcgaggactc
     1921 gcccaaccgc atcgtcgtcg ctcccgatgc catgacttat tgggataccc tgcgcgaggg
     1981 cttccttgat caggccacgg tgcatatata tcgcatcgag aacggccgtt tcaggatggc
     2041 gcgcgaagac cgggtcacga gcggtggcgc gcacgccagc ggctggttcc gcgccctcgc
     2101 caacaaccag gtcgttacgc ccggagtcac ccgcttcaga tttcgttggg atgactggaa
     2161 ccgaccgcgc gccaggtact tctttaccgt gcgcgccatc gacaagtacg gtggcctgtc
     2221 tcctcctgcc tctgccttcg aaatcgaaag cccggaaagg ctgaacaaag caccggcggc
     2281 acaaaatgcg ctcgtcacct tcaatcccgt caaggcgctt ttctccaatc cgacgcctcc
     2341 acccgcaccg ggaaacttca gaggtaaact cgacagtgac ggaatcctga cactggaatg
     2401 ggaaccggtc gactcgcccg atgtggccgg ctatgtcgtc tatcgttccg actatccgcc
     2461 gcaacgacac agcggctact atctccagct ggccggcacg cccacctcgg cggaacagaa
     2521 catcaaggcg ggcgacatgg ttatcgtcag caagaaaatc tattcgccct cgcgcaatcg
     2581 agatatatcc aaccgggtct ggggagcatc aaatgaatac gacaagctga tgccggggct
     2641 aatccacttc ttccccgacg aaactcccgg caagacatgg tcgctcgttc cgcacgccgc
     2701 caatacgccg gtcgatgaac ctggcgaaac ctgtctcaag ctgcagctcg cggcggacgc
     2761 cagcgaatcg ctgagtatct acaaccacag cggcaccggt cagttctggt acgacgtgct
     2821 ggagaagaaa tcctatacgg tcgaggtctg gatgcgccag gaaggcagcg gcacggtaca
     2881 gttcaagttg ggcggtttct acaaagacgc accacaaaga attgagccga tcacctttaa
     2941 cgttggccca gtctggaaga aatacgtcgc gcattttacg cccacagtga tccaaagtgg
     3001 ggcccgcccc aacgacatgg tgctgaaatt caccggccca gccacctttt atgtcgacaa
     3061 tttccgcatc tatcggacgg acacggccta tctggagatg ctgccgcacg aatacagcgc
     3121 catcaagtcg tccggcattt tatccttgcg cacctcgggc ctgatcatga cccgtaccca
     3181 cacctataac atggagcagc tcaccaacag cggtggtgtg attaacggaa cacagaaatt
     3241 aaacaccctg ccccagacct tgaagataat gcgcaaggcc ggcgtgcggc cctggctgca
     3301 aattgagttc catatgtcac cccaagagtg gctgggtttt gtcgaataca tggctgctcc
     3361 gtacgatccg aaagttgaca ctcccgccag taagccatgg gcctacaagc gctaccgcca
     3421 ggggcaggcc agaccttggg tcgaggagtt cgaccagatc tatttcgaat tgagcaacga
     3481 gacctggaac cgcatcttcc agccatggat attcaacccc atgaccgacg ccgtgtcgaa
     3541 gaagcgctac agccccggtc aggtttatgg cctgtttcag gaatacgttc actcgatcat
     3601 gcgaagtagc ccctattggc ggcctgcgga cctggacaga aaatttgatt tcatactggg
     3661 cggttgggag agcgtcatgg gctacagccg tgacgcggct tccgtttccc cttcatcgga
     3721 ctttctcacc atcgcgcctt acatcggcgg ctcggatgcg gcagaaggcc cgcctaagcc
     3781 gggtgccgcc agcctgttca acgtcctggc ccaggtcaac cagtcggcca ttccggtggc
     3841 ggacctctat gcgaaagaat tgctcgacct gaatagcaaa ggcgcgcgca agttgcgcct
     3901 gggaacttac gagggaggac caggctacgt tatgaatggg ctcaacaacg cccgcgtaac
     3961 cgaagaggag gcgcatgctc aggagcaggt catgaaaagc atggcggctg gtacggcaac
     4021 gctggattca ttcctggccc gggcctaccg gggctttgcc gtacagaatt tcagcgcatt
     4081 tgacagcggc gatcggtgga agtcgcatgc gaaatggtat cacggcgggc aggcctaccc
     4141 atcctggaag ctgctcgcct tgttcaataa cgaagccggc ggcgatgaca tgctgcgcac
     4201 cgagaccctg agcgtcccga ccgcggaact cagggcgttc agccgccgac agaccatcaa
     4261 ggacgcgcct ctggcagcga tctacgccac gcgcaaagaa gaccgctaca atctttttgt
     4321 aatctcccgc aaggtaccgg gctatcccgt cgtcggagac gatggctaca ccccggtcac
     4381 cgtcgaactt cccttcagca aggcaaaatc catcacgctt taccggatga caggcgctcc
     4441 ggatgcgaac aatctgatgg gtgacaatgt caggatcgag aaa
//
LOCUS       DBNGZE010000241         6198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1213649, whole genome shotgun sequence.
ACCESSION   DBNGZE010000241 DBNGZE010000000
VERSION     DBNGZE010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1213649"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC16FV_06280"
     CDS             <1..215
                     /locus_tag="AC16FV_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849699.1"
                     /translation="NGAFIEGTPEGLPYFNAPVELGLTLTSGGETKQHRIPAKIRRIT
                     DNGVGLTFGDVGMDAYFSLVNLVYNA"
     gene            604..3006
                     /locus_tag="AC16FV_06285"
     CDS             604..3006
                     /locus_tag="AC16FV_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR2849700.1"
                     /translation="MFRYKSKYLIASGFVLVLALTVALAGVGLTRMTAIHNHLKLITE
                     KHNAKADIIFSMRHVARERALSTYAMFIMDDPFDREEELRRFTDMAGEFIHLRDRLIE
                     IGLEEKEQLALDKVLEQVRLYQPLHLALVDRITSERLAGVKEEILRHDLLRQRTMLGL
                     LDEMVELERVESEKAAAQAAREYNSAYLTMLALTAVIIISGILIAAYVIYCTRRVEQA
                     LAREKEHAEITLHSVGDAVIAADEQGNVNYLNPAAEQMTGWRNEEARGQPLRNIYHII
                     NETTRKPLEHPAMLGVLDTPIAGLDKHTLLISRSGRELAIEDNAAPIRNSDGEDIGAI
                     LVFRDVTEQRQMQKQLSWQASHDPLTGLANRREFEVLLERLISSAKEQDKRHALLYLD
                     LDQFKVINDTCGHVAGDELLRQIAGLMRPLIRDSDTLTRLGGDEFGILLEGCYITQAE
                     QIAHKVLETLEDFRFIWQDKTFRVGVSIGLVGIHAGSRTASSMLSAADGACYMAKDKG
                     RNRIWVHQDDDHETAHRQGEMEWVSRIMRAFDDNRFVLYFQRVLPLTTKADDPSYREV
                     LVRMRDENGELVPPMAFIPAAERYGVMSTVDRWVVKTAFAWLAIHPAEERLAINLSSQ
                     SLGDEDFLKFVMEQFRTTRLSPRRICFEITETAAIANWHRATHFIAALRTLGCRFALD
                     DFGSGMSSFGYLKSLPIDIIKIDGAFVRDMIDDEVDFAMVEAVNRIGHVMGIRTTAEY
                     VENDKILEKLRELGVDYAQGYGLHVPQPLEKITATTAGKVTTTGKKPFDMDMPVVGRP
                     AI"
     gene            complement(3003..3737)
                     /locus_tag="AC16FV_06290"
     CDS             complement(3003..3737)
                     /locus_tag="AC16FV_06290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR2849701.1"
                     /translation="MTDPLFYSEQLADPGATLVLTGDEAHHAAASRRLHDGDTLWLFD
                     GHGSIARTTLLHATARGRTLELHVEERRTEPPPRPAIHLACALPKGDRQNVLLDMATQ
                     LGMTRFTPLDCERSVVRPGASSMVRWRKICLEACKQSRRLYLPAIEATSTVRDVVERT
                     TVERGKVWLAHPAAEAVTVSTAVNQDTNDVTILVGPEGGFTEKEVEQAVAAGAKLLAL
                     GTTILRIEAAAVALVAAFALKTRSDD"
     gene            complement(3855..4673)
                     /locus_tag="AC16FV_06295"
     CDS             complement(3855..4673)
                     /locus_tag="AC16FV_06295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2849702.1"
                     /translation="MTPILFRRPSAVPVHPHPATIRIAVGFNGDAALKTRAEELAREL
                     SLPLTVAGDIDSDLQLVLTPEYLELRDCRDSRLGPVCVDFSHLDLRPYSANLSRRQPL
                     ARAFGKKTHTIVDATAGYAQDALLLALMGFHVTAIERSPVVTALARDGLQRLTSSTGL
                     ALSERLHLVNGDSRVLLPAITPRPDAIYLDPMFPPKRRKSAAVKKEMRLLRELVGDDA
                     DAPELLDLSRAIARDRVVVKRPDDALPLAPEPSMSLTGKLVRYDVYLTREPKNK"
     gene            complement(4783..5295)
                     /locus_tag="AC16FV_06300"
     CDS             complement(4783..5295)
                     /locus_tag="AC16FV_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR2849703.1"
                     /translation="MRRRLYFVLPDVKTAKAVFKELLLARIEQQHVFFLAKEGMALND
                     LPEATMFQKSDEIHGLALGLVVGGATGALAGVVAMMFPPSGLAIGLGVILAMSLIGAI
                     VGIWASGMIAADVPNTRLRKFSRDIEQGRILLMVDVPKDQVGEISSMIRDQYPDAAAG
                     GRDPTIPAFP"
     gene            complement(5464..>6198)
                     /locus_tag="AC16FV_06305"
     CDS             complement(5464..>6198)
                     /locus_tag="AC16FV_06305"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0097216 - guanosine tetraphosphate binding
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit assembly factor BipA"
                     /protein_id="HIR2849704.1"
                     /translation="ELHLGILIENMRREGYELGVSRPQVIVKMVNNEPHEPFESVTVD
                     IEQQHQGVVMEKMGLRRGDLQNMEPDGKGRVRLEYLIPSRGLIGFRNEFLTATQGSGL
                     LYSIFDHYGPVKKGEVTHRVNGVLISNGQGPSVAYSLFSLQERGRLFIGPGEELYEGM
                     IIGIHSRDNDLVVNPLKEKKLTNIRAAGSDENILLTPPIELTLEKAIEFIDDDELVEV
                     TPNHIRLRKRFLKETDRKRAERAAAG"
BASE COUNT         1249 a         1827 c         1844 g         1278 t
ORIGIN      
        1 tgaatggcgc ttttatcgag ggtaccccgg agggactgcc gtatttcaat gcccccgtgg
       61 aactgggtct gaccctgact tccggcggtg agaccaagca gcatcgcatc cccgccaaga
      121 tccgccgcat taccgacaat ggcgtcggcc tgactttcgg tgacgtcggg atggacgcct
      181 atttcagtct ggtcaatctg gtctataacg cctgaattct ggctttttcc tgtccatttt
      241 ctggttctct tcaccgtccg gttgctttcc ggacggacga aatccagtcg gttgccgttt
      301 tatgtaagcc tgccgccgtt ttccggcctg aatttggtga cctcaggcta aacctgtttc
      361 tgatcgctat actctaacca cctgtttcta cactgtttcc tgcagatatc tgccgctgaa
      421 tcatttaagg cacgtccctt gcacagaatt agccccaagg gacggggata accactcaat
      481 tgcgcgtcac ttcggatacc ggattcgaag gcgaagggcc ggtcagagca agttacgggc
      541 aaggttatcg gcgacaggcg gcaaagcaac ctggcagtca ttggccgggt gactgtattt
      601 ttcatgtttc gctataagtc aaagtatctc atcgcctcgg gcttcgttct ggttctcgcg
      661 ctcacggtcg cgcttgcggg cgtcggcctc acgcgcatga ccgcgatcca caatcacctc
      721 aagctcatca ccgagaagca caacgccaag gccgatatca tcttctcaat gcgccacgtc
      781 gcccgtgaac gggcgctcag cacctacgcc atgttcatca tggacgatcc cttcgaccgc
      841 gaggaggaac tgcgccggtt caccgacatg gccggtgagt tcatacatct gcgtgatcgg
      901 ctgatagaga ttggtctcga agagaaggaa caactggcgc tggacaaggt gctggaacag
      961 gtgcgcttgt accagccgtt gcacctggcg ctggtggaca ggattacgag cgagcgcctc
     1021 gcgggcgtga aagaggaaat cctgcggcat gatctgctgc gtcagcgcac catgctgggc
     1081 ctgctggacg agatggtgga actggagcgt gtcgaaagtg aaaaggccgc ggctcaggcc
     1141 gcccgggaat acaattcggc ctatctgacc atgctcgctc tgaccgccgt tatcattatt
     1201 tcgggcattc tcattgccgc ctatgtcatt tattgcacgc gccgggtgga acaggcgctg
     1261 gcacgggaga aggagcatgc cgagatcaca ctgcattcgg tgggcgatgc cgtcattgcc
     1321 gccgacgaac aaggcaacgt caattacctc aacccggccg ccgagcaaat gacaggttgg
     1381 cgcaacgagg aggcacgcgg ccagccgttg cgcaacattt accacatcat caacgaaacc
     1441 acgcgcaagc cgctcgaaca tcccgcgatg ctgggcgttc tggatacgcc catcgccgga
     1501 ttggacaagc acaccctgct gattagtcgc tccggccggg agctggctat cgaagacaac
     1561 gccgcgccga tccgcaacag cgatggggag gacatcgggg cgatcctggt gttccgcgac
     1621 gtgaccgagc agcgtcagat gcagaaacaa ctgagctggc aggccagcca cgacccgctc
     1681 accggattgg ccaaccggcg cgaattcgag gtgctgctgg agcggctgat ctcaagcgcc
     1741 aaggaacagg acaagcgcca tgccctgttg tatctcgatc tcgaccagtt caaggtgatc
     1801 aatgacactt gcgggcacgt cgccggcgac gaattgctgc ggcagatcgc cgggctcatg
     1861 cgtcctctca tccgcgacag cgacacccta acccgtctcg gcggcgacga attcggcatt
     1921 ttgctcgagg gctgctacat cacgcaggcg gagcagatcg cgcacaaggt gctggagacg
     1981 ctggaagatt tccgtttcat ctggcaggac aagaccttcc gcgtcggcgt ttccatcggc
     2041 ctggtcggta tccatgctgg cagccgcacg gcttcgagca tgttgagcgc cgccgatggc
     2101 gcctgctata tggccaagga caaggggcgc aaccgcatct gggtgcatca ggacgacgac
     2161 catgaaacag cgcaccggca aggggaaatg gaatgggtct cgcgcatcat gcgtgcgttc
     2221 gatgacaacc ggttcgttct ctacttccag cgcgtgctgc cgctcacgac caaggcggac
     2281 gatccatcct accgcgaggt gctggtgcgc atgcgcgatg aaaatggcga gctggtgccg
     2341 ccgatggcgt tcatcccggc cgccgagcgt tatggcgtga tgagcacggt cgatcgctgg
     2401 gtcgtcaaaa ccgcctttgc ctggctggcc attcaccccg ccgaagagcg gctggccatc
     2461 aatctttcca gtcagtcgct gggtgacgag gatttcctga aattcgtgat ggagcagttc
     2521 cgcaccacgc gtctgtcgcc gcgccgcatc tgtttcgaga tcacggagac cgcggccatc
     2581 gccaactggc accgcgccac gcatttcatc gccgcgctgc gcaccctcgg ctgccgcttc
     2641 gccctcgatg atttcggcag cggcatgtcg tccttcggtt atctgaaaag cctgcccatc
     2701 gacatcatca agatcgacgg cgcgttcgtg cgcgacatga tcgacgacga ggttgatttc
     2761 gccatggtcg aggcggtcaa ccgcatcggc cacgtcatgg gcatccgcac cacggcggag
     2821 tacgtggaaa acgacaaaat actcgagaag ctgcgtgaat tgggcgtgga ctatgcgcag
     2881 ggttacggcc tgcatgtgcc tcagccgctc gagaaaatta ccgcgaccac ggcagggaaa
     2941 gtaacgacca ctgggaaaaa gccgttcgac atggacatgc cggtggtggg tcgccccgcc
     3001 atttaatcgt cggatctggt ttttagcgca aaggcagcga ccagtgccac ggccgcggct
     3061 tctatccgca atatggttgt tcccagcgcg agcaattttg ccccggccgc gacagcctgt
     3121 tcgacctctt tttccgtgaa cccgccttcc gggccaacca gaattgtcac gtcattcgta
     3181 tcctgattaa ccgcggttga aaccgtcaca gcctctgcgg cggggtgggc gagccacact
     3241 ttgccgcgtt ccaccgtcgt gcgttcaaca acatcccgta ccgtggatgt cgcttcaatg
     3301 gccggaaggt acaaccgccg gctctgtttg cacgcttcga gacagatttt tctccagcgc
     3361 accatgctgc tggcgccggg tctgaccacg ctgcgttcgc aatcgagtgg cgtgaatcgc
     3421 gtcatgccga gctgggtcgc catgtcgagc agcacgttct gacgatcgcc cttgggcagg
     3481 gcgcaagcca gatgaatcgc cggccggggc gggggttcgg tacgccgttc ctcgacgtgc
     3541 aattccagtg tccgtccgcg cgccgtggca tggagcaacg tggtgcgcgc gatgctgcca
     3601 tgcccatcga aaagccacaa tgtatctcca tcgtgaaggc ggcgcgaggc ggcggcgtga
     3661 tgcgcttcgt ctcccgtcag caccagcgtc gcaccgggat cggcgagctg ttcggaataa
     3721 aatagcggat cagtcatatt ttgggaaaaa aatttcacag gatttacagg attaaaagca
     3781 ggattaacag gattatttct ttaaatttta aaatcctcaa tcctgtaaat cctgaaaaat
     3841 cctgttaatc ctgtttattt gtttttcggt tctcttgtga gataaacatc gtagcgcacc
     3901 agtttacccg tcaggctcat gctcggttcc ggcgcgagcg gaagcgcatc gtcgggccgt
     3961 ttcacgacca cgcggtcgcg ggcaatggca cggctgaggt caagcaattc cggcgcgtcc
     4021 gcatcgtcgc cgaccagctc gcgcagcagg cgcatttctt ttttcaccgc ggcggatttg
     4081 cgccgtttcg gcgggaacat gggatcgaga tagattgcat cgggacgtgg cgtgattgcg
     4141 gggagcagta cacgcgagtc gccattaacg agatgcaaac gctcggacag cgctagtcct
     4201 gtcgatgagg tgaggcgttg caggccgtcg cgcgccagcg ccgtgaccac cggcgaacgc
     4261 tcgatagccg tgacgtgaaa acccatgagc gccaacaaca acgcatcctg ggcatagccg
     4321 gctgtggcat ccacgatggt atgggttttt ttaccgaagg cgcgcgccag cggctggcgg
     4381 cgtgagaggt ttgcgctata ggggcgcagg tcaaggtgcg agaaatccac acagaccgga
     4441 cccaaacgcg aatcgcggca atcacgcagc tccaagtatt cgggcgtcag gaccagttgc
     4501 aaatcagaat ctatatcccc tgccaccgtc aaaggcagcg acagctcgcg tgccagttcc
     4561 tcggcgcggg ttttcaaggc ggcatcgccg ttgaaaccaa cggcgatgcg aattgtggcg
     4621 gggtgtggat ggacgggaac tgctgatggg cggcggaaca gaataggtgt catgatgcgt
     4681 ctagcataat cactctcgcc ttgccgcgca cccgcgagtc gatggcacaa agcaatatgc
     4741 cccgttacac ggggcatatt gggtacgaca gcggattttt ctttaaggaa acgccggaat
     4801 ggtcgggtcg cgtccgcctg cggcggcatc cggatattgg tcccggatca tgctgctgat
     4861 ttccccgacc tgatccttgg ggacgtccac catcagcagg attcttccct gttcaatatc
     4921 cctggaaaat tttctgagac gagtgttggg aacatccgcg gcgatcatgc cggaggccca
     4981 gatgccgacg atggcgccga tcaagctcat ggccaggatg acacccaggc ctatcgccag
     5041 gccggaaggt ggaaacatca tggcgacgac gccggcgagc gccccagtgg cgccgcccac
     5101 gactaggccc agggcaagac cgtggatttc atcgctcttc tggaacatgg tcgcctcggg
     5161 caagtcgttc aacgccatgc cttccttggc cagaaaaaat acatgttgtt gttcgatgcg
     5221 ggccaacagc agttccttga atactgcctt ggcggttttg acgtcgggga gcacaaaata
     5281 tagccgtctt ctcatcgcaa cctccttccc gcaacgggga ctggtgtcag acagagacca
     5341 ctttacgcct gtggcaacaa ataaagcata gccgaagtgc gacggcatcc caacgagatg
     5401 ccgtactttt cgtgtcagat cttccgggag gatggcgcga cgagtgccgt aacgccagcg
     5461 gaatcaaccg gctgcggcgc gttccgcgcg cttgcggtca gtttctttca gaaagcgctt
     5521 gcgtagccga atatggttcg gcgtgacctc gaccagctcg tcgtcgtcga tgaactcgat
     5581 ggccttttcg agcgtgagct cgatcggcgg cgtgagcagg atattctcgt ccgagccggc
     5641 ggcgcggatg ttggtgagct ttttctcctt gagcggattc accaccagat cgttgtcgcg
     5701 cgaatggatg ccgatgatca tgccctcgta cagctcttcg cccgggccga tgaacagacg
     5761 cccgcgttcc tgcaggctga acagcgagta ggcgacgctg gggccctggc cgttcgagat
     5821 cagcacgccg ttgacgcgat gtgtgacctc gccctttttc accggaccgt agtggtcgaa
     5881 gatggagtag agcaggcccg agccctgggt ggcggtgagg aactcgttgc gaaagccaat
     5941 gaggccgcgc gacggaatca ggtattcgag gcgcacgcgg cccttgccgt ccggttccat
     6001 gttctgcagg tcaccgcggc gcagtcccat cttctccatc accacgccct ggtgctgctg
     6061 ttcgatatcg accgtgactg actcgaacgg ctcgtgtggt tcgttgttga ccattttcac
     6121 gatcacctgc gggcgcgaca cgccgagctc atagccctcg cggcgcatgt tctcgatgag
     6181 gatgccaaga tgcagttc
//
LOCUS       DBNGZE010000242         8913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1700348, whole genome shotgun sequence.
ACCESSION   DBNGZE010000242 DBNGZE010000000
VERSION     DBNGZE010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1700348"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16FV_06310"
     CDS             <1..384
                     /locus_tag="AC16FV_06310"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HIR2849705.1"
                     /translation="LARNEAIKRNTPITLCKSSDGASCSDEAAWRDGWIVFTDHNKNR
                     EVDANEAIIRVQQALTGNMTLRYGNTGTYTYVRYNSSGEASRAATFTFCDGRGADKAK
                     AVIVYWTGRPRISTRTSEDESLTCT"
     gene            524..599
                     /locus_tag="AC16FV_06315"
     tRNA            524..599
                     /locus_tag="AC16FV_06315"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Asn,seq:gtt)
     gene            complement(614..1033)
                     /locus_tag="AC16FV_06320"
     CDS             complement(614..1033)
                     /locus_tag="AC16FV_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR2849706.1"
                     /translation="MSDNTLLTEKPALTLAAARKIIAAAETEALKQDWPVVIAVVDDG
                     GHLICLHRLDNTQFGSVEIAIQKARSAVAFKRPTKAWEELLVSGQHRVLGLPGVLSSE
                     GGVPLIWKGQIVGAIGVSGVKSSEDGQVARAGAGILE"
     gene            1165..2040
                     /locus_tag="AC16FV_06325"
     CDS             1165..2040
                     /locus_tag="AC16FV_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR2849707.1"
                     /translation="MLTLRLFAIVAPIFAIIAIGYAYGRWRQPDMTAANQVNMTVLLP
                     ALLFHVLSGKDFQIGAYGPLALGGVAVLLGSGLLALPIAKLARLPFKTFVPPMMFNNA
                     GNIGLPLAVFAFGEQALPAAVVLFLIENGLHFTLGVYLMDHRAPLYKVFMQPIVIATL
                     IGIAFGIFKWPVPPPLAIAIDMLGQASIPLLLFALGARLTSIDFGQWRVGVLGAVACP
                     LTGVLVVLLVRPFLDLSPLQSSLLLVFGALPPAVLNYLVAEQYRQEPGKVASIVLIGN
                     LAGFVSLPIALAFAL"
     gene            complement(2063..3775)
                     /locus_tag="AC16FV_06330"
     CDS             complement(2063..3775)
                     /locus_tag="AC16FV_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protein"
                     /protein_id="HIR2849708.1"
                     /translation="MRILFLTHSFNSLTQRLYVELTERGHEISIEFDINDAVTIEAVR
                     LFRPDLIVAPYLRRAIPEAIWRHHLYLVVHPGIVGDRGPSALDWAILNGKTEWGVTVL
                     QANSEMDAGPVWASVNFTTRATRKSSLYRNEVTEAAVTAVLQAIERFEQRSFTPSLPD
                     SGARGQWHPLMNQSDRHIDWLQDDTRTVLRKINSGDGAPGVADMLGEELYHLYDACEE
                     ESLGGKPGEIIARRHGAICRATRDGAVWIGHLKKIIPGEKTFKLPATVVLNGALARVP
                     EAPLPLDDSRDSKTYKDIWYKERDGVGYLHFDFYNGAMSTEQCQRLLEAYKQVRQRPT
                     RVIVLMGGQDFWSNGLHLNRIEAADSPADESWRNINAMDDLVQAIITTNTQLTIAALR
                     GNAGAGGAFLALAADRIYARAGVVLNPHYKNMGNLYGSEYWTYLLPRRVGEHGREIME
                     NRLPLGASAAKRFGLIDDCFGKDADGFAAQIESIAVALAQDASYETLLMEKHQRRMRD
                     EADKPLAQYRTEELERMKLNFYGFDPSYHVARYNFVFRVPNSWTPLHLARHRRASRKI
                     AATA"
     gene            complement(3779..4960)
                     /gene="hypD"
                     /locus_tag="AC16FV_06335"
     CDS             complement(3779..4960)
                     /gene="hypD"
                     /locus_tag="AC16FV_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR2849709.1"
                     /translation="MKYINEFRDRRVATALAAELAAEADPAREYSLMEFCGGHTHAIF
                     RYGVQDLLPPNVHLVHGPGCPVCVLPISRLDMAIELAEVPDVILCSYGDMLRVPGSRK
                     RSLLKSRAQGRDVRMVYSCTEVIQIAQDNPDKEVVFFAIGFETTTPPTAVAIKQAEML
                     GLKNFSVFCNHVLTPSAIQSILDSPDVRKLGSVRVDGFIGPSHVSTVIGSRPYDFFAQ
                     EYQRPVVIAGFEPLDVLQAIGMLVRQLNEGRAEVENQFTRGVTHAGNLKAQALVAEIF
                     ELRRSFEWRGLGVVPYSGLRIKKQYATYDAERRFPLAQKPVQENKACECGAILRGVKK
                     PTDCKIFGTVCTPQNPIGSCMVSSEGACAAYYKYRRHGGITETTQTEGKSAGVNIVSG
                     G"
     gene            complement(4944..5192)
                     /locus_tag="AC16FV_06340"
     CDS             complement(4944..5192)
                     /locus_tag="AC16FV_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR2849710.1"
                     /translation="MCLAIPAKVVQKLDNDRAVVEVGGVRNDISLMLVDGVEVGDYVI
                     VHVGFAITRLDVDEAEKSLALFDEIARHLETRPHEIHQ"
     gene            5454..7742
                     /gene="mrcB"
                     /locus_tag="AC16FV_06345"
     CDS             5454..7742
                     /gene="mrcB"
                     /locus_tag="AC16FV_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HIR2849711.1"
                     /translation="MTKFRNFFTRKRVRLLLLSLALLLSGFALYLDITVRTHFEGKRW
                     AMPARVYARPLELYPGMKLRPEQLAMELAMLDYHAAGDPQSPGNYQRQGDEFTLVTRP
                     FTFWDEAQASLPLQIDFDGAHLGTLTNRSTGKPVTLARLDPVLIGGIYPAHNEDRVLV
                     KFDEIPPHLVKALIAIEDRQFYSHHGFSVRGTARALVSILSGSGVQGGSTLTQQLVKN
                     FFLTPERTLRRKFTEVIMAMLLELHYSKDEILEAYANEIYLGQDGNRAIHGFGLASHF
                     YFDRPLSRLDLPQAALLVGLVKGPSSYDPRRQPARALARRNLVLQEMRKLDYITQAQF
                     VAARAASLGVAEKAPEGTSLYPAFVQLVHRQLRRDYREEDLRSEGLQIFTTLDPLVQT
                     SAERALTTRLAQLERDRRIPPKALEGAIVVSNSQNGEVQALVGGRNTRFEGFNRALDA
                     ARPIGSLIKPVVYLTALERPASYTLATLLDDSPLVWRQRGTSDWEPQNYDKQFHGQVP
                     LRVALANSYNVSTARLGLALGVRHVVDSAHRLGVERDMNAYASTLLGADSLSPLEVTQ
                     MYQSFASGGFRVPLRAIREVLMADGQPLQRYALSVEQVIEPGPAYLVTRALQTVVQEG
                     TADALKKYLSSDLHLAGKTGTTNDLRDSWFAGYTGDRLAVVWIGRDDNQPINMTGAGG
                     AMTVWGEMMARLDPDPLIPPLPENIETISIDPVSGLRADANCQEAVELPFIRGSGPTE
                     TAPCATHSPARSIKSWFERLFE"
     gene            7739..8164
                     /locus_tag="AC16FV_06350"
     CDS             7739..8164
                     /locus_tag="AC16FV_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849712.1"
                     /translation="MSKQRSIILFFIAAASVAGCAGFPPTAPEAQPVSDNTAVVALMD
                     TARTDIANGKLDAAVAPLERALRIEPRNPMLWQELARLRLQQGQYQQAEGMATRSNSW
                     AGDNKTLRAENWRIIGEARLKRGDHQGAQAAFDKAATQN"
     gene            8243..8635
                     /locus_tag="AC16FV_06355"
     CDS             8243..8635
                     /locus_tag="AC16FV_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA family protein"
                     /protein_id="HIR2849713.1"
                     /translation="MIQRGWLFLLFLVLTGCATPEAEILSKVESFPPTLKVEVLLDRP
                     TRPHKTIAILGDVYGGTPEEVNERLSRKAREIGADAIVIMSVHDKSTTDWLLDDPFYY
                     PHGVHWPRYRPVRYTYRIVRARAIKYTE"
     gene            8632..>8913
                     /locus_tag="AC16FV_06360"
     CDS             8632..>8913
                     /locus_tag="AC16FV_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR2849714.1"
                     /translation="MTAENLWLQGGGLVTGLIFGGIVQRQRFCMVAAIGNLVLMRDWR
                     HTHAFLAAFAVAIAGTQWLEASALVPVAESAYRKARFDWLGATAGGLIFG"
BASE COUNT         1719 a         2795 c         2601 g         1798 t
ORIGIN      
        1 ctcgcgcgca acgaagccat taaacgcaat acgcctatta ccttgtgcaa aagcagcgat
       61 ggcgcttcct gttcggatga agccgcttgg cgcgacggct ggatcgtctt taccgaccat
      121 aataaaaacc gtgaagtgga tgccaacgag gcaataatcc gcgtgcaaca ggcgttgacg
      181 ggaaatatga ccttgcgtta tggcaacacc ggcacttaca cctacgtgcg atacaattcg
      241 tccggcgagg cgtctcgggc cgccacattt accttctgtg acggtcgcgg cgcagataaa
      301 gccaaagccg tgattgtcta ctggaccggc cgtccgcgca tctcaaccag aacgtcagaa
      361 gatgagtcgc tgacctgcac ctagccgctt gccttgaccc tgcccgtcat ccgccctaac
      421 atacgcgccc ttctcgtctt atggcaagac gagcgagcga agcgagtagg gcttctcgtc
      481 atatgacagg acgagcgagc atagcgagca gggcttcatt tattccccgg tagctcagtc
      541 ggtagagcag gtgactgtta atcaccgggt cggcggttcg agtccgtccc ggggagccat
      601 ttccttcatc tcattactcc agaattcctg ccccggcacg ggcgacttgg ccatcttctg
      661 aggatttaac cccgctcacg ccaatggccc cgacgatctg gcccttccag atcagaggca
      721 cgccgccttc ggacgacaag actcccggca atccaagaac acgatgctgg ccgctcacca
      781 gcaactcttc ccaggctttg gtcggtcgtt tgaatgccac ggctgatcgc gccttttgaa
      841 tggcgatctc gacactgccg aactgggtat tatcgagacg gtgcagacaa atcagatgac
      901 cgccgtcgtc caccacggcg atgaccacgg gccagtcctg tttcagcgcc tcggtttccg
      961 cagcggcgat aatcttcctg gcagccgcca gcgtcaacgc cggtttttcg gtcagcaggg
     1021 tgttgtccga catgtctcct ctcctcgtgg tatccgtacc ggcgaataag ataccgggaa
     1081 ggcgttctga tgcaatcgtt atcgcatcgc gggaaaccgg gttttgccaa gccgcgtgaa
     1141 acacacgcat aatcccgttt cgccatgttg accctccggc ttttcgccat cgtcgcaccg
     1201 atcttcgcga tcattgcgat aggctatgca tacggccgct ggcgccagcc ggacatgacc
     1261 gcggcgaacc aggtcaacat gacagtgttg ttgccggcgc tgctgtttca cgtgctttcc
     1321 ggcaaggatt tccagatcgg cgcgtacgga ccgctggccc tcggcggagt cgccgtcctg
     1381 ctgggctcgg gcctgctggc gctgccgatc gcgaaactcg cgcgcctccc gttcaagact
     1441 ttcgtgcctc cgatgatgtt caacaacgcc ggcaacatcg gcctgccact cgcggtgttc
     1501 gccttcggcg agcaagcgct gcccgcggcg gtagtgctgt tcctgatcga aaacgggctg
     1561 cacttcaccc tgggcgttta cctcatggac caccgcgcgc cgctgtacaa ggtattcatg
     1621 caacccatcg tcatcgccac gctcatcggc atcgccttcg ggatattcaa gtggcccgtg
     1681 ccgccgccac tggccatcgc gatcgatatg ctcgggcagg cgtcgatacc gctgctgctg
     1741 ttcgcgctgg gcgcgcgcct caccagcatc gacttcgggc aatggcgggt cggcgtgctc
     1801 ggcgccgtcg cctgtcccct gaccggtgtg ctggtggtgc tgctggtgcg gccgtttctc
     1861 gacctgtcgc cgttgcaatc gagtctgctg ctggtgttcg gcgccctgcc gccggcggtg
     1921 ctgaattatc tggttgccga gcaataccgg caggaaccgg gcaaggtggc ctccatcgtg
     1981 ctgatcggca atctcgctgg ttttgtcagc ctgccgattg cgctggcctt cgcgctctaa
     2041 acgcaggatc agctgaactg aattatgccg tcgccgcgat cttccggctt gcacgccggt
     2101 gtcgcgcaag atgaagcggc gtccaggaat taggaacccg gaatacgaag ttatagcgcg
     2161 ccacgtgata actgggatcg aaaccgtaaa aattgagttt catgcgctcc agttcttcgg
     2221 tgcggtactg cgccagcggt ttatcggctt cgtcccgcat acgccgctgg tgtttttcca
     2281 tcagcagtgt ttcgtacgat gcatcctggg cgagtgccac cgcgatagat tcaatttgcg
     2341 cggcaaaacc atccgcatcc ttgccgaaac aatcgtcgat aaggccgaaa cgcttggcgg
     2401 cactggcgcc cagcggtaga cggttctcca tgatctcacg tccgtgctct cccacccgcc
     2461 gcggcaggag ataggtccag tactcggaac cgtagagatt tcccatgttc ttgtagtgtg
     2521 gattgagcac cacgcccgca cgcgcataaa tccggtccgc cgccaaggcc agaaaagccc
     2581 cgcccgcccc ggcattgcca cgcagggcgg ctatggtcag ttgcgtgttg gtcgtgatga
     2641 tggcctgcac cagatcatcc atggcgttga tgttgcgcca ggattcgtcc gccgggctgt
     2701 ctgcggcttc gatacggtta agatgaagcc cgttcgacca gaagtcctgc ccacccatca
     2761 ggactatcac ccgcgtaggg cgctgtcgaa cctgtttata tgcctcaagc aaacgttggc
     2821 actgttcggt gctcatcgca ccgttgtaaa agtcgaaatg caggtaacct accccatccc
     2881 tctctttata ccagatgtct ttatatgtct tgctatcgcg ggagtcgtcc agcggaagtg
     2941 gcgcctcagg cacgcgcgct aatgccccgt tcaacacgac cgtggcgggc aatttaaaag
     3001 tcttttctcc cggaatgatc tttttcaaat ggccgatcca cacggctcca tcgcgggtcg
     3061 cgcggcagat ggcgccgtgg cggcgcgcga tgatttcacc cggcttacca cccaggcttt
     3121 cctcttcaca ggcatcgtat aaatggtaga gctcttctcc cagcatatcg gccacgcccg
     3181 gcgcgccatc gccggagttt attttccgta acacggttcg ggtatcgtcc tgcaaccaat
     3241 cgatatgacg gtcggattga ttcatgagcg ggtgccattg accgcgagcg ccagagtccg
     3301 gcaatgacgg cgtgaaactc ctttgttcga aacgttcaat cgcctgcaac accgccgtga
     3361 cggcagcctc ggtcacttcg ttgcggtaca gactgctttt gcgcgttgcg cgcgttgtga
     3421 aattgaccga agcccaaacc gggccggcat ccatttcact attggcctgc agcacggtga
     3481 caccccactc tgttttaccg ttcaggatcg cccagtcgag ggccgagggg ccgcgatcgc
     3541 caacgatgcc ggggtgcact accagataaa ggtgatggcg ccagatggct tcgggaatag
     3601 cgcgccgcag atacggcgcc acgatcaggt ccggccgaaa cagccgaacc gcttcaatag
     3661 ttactgcatc gttgatgtcg aactcgatcg aaatctcatg cccgcgctcc gtcagctcga
     3721 catacaggcg ttgcgtcagg ctgttgaagc tgtgcgtgag aaaaaggatg cgcatgcatc
     3781 atccgcccga gacgatattg actccggcgc tcttgccttc cgtttgtgtc gtctctgtta
     3841 tgcctccgtg gcgccggtat ttgtaatacg ccgcgcaggc gccttccgag gacaccatgc
     3901 aggagcctat cgggttctgc ggcgtgcaaa ccgtgccgaa aatcttgcag tcggtgggct
     3961 tcttcacgcc gcgcaggata gcgccgcatt cgcaggcctt gttctcctgt accggtttct
     4021 gtgccagggg aaaacgcctc tcggcgtcat aggtcgcata ttgctttttg atgcgcaggc
     4081 cgctgtaggg aaccacaccc aaaccgcgcc attcgaacga gcgccgcaac tcgaagattt
     4141 ccgccaccag cgcctgcgcc ttgagattgc cggcgtgcgt gacaccgcgg gtaaactggt
     4201 tttcaacctc ggcacgacct tcattcaact ggcgtaccag catgccgatc gcctgcaata
     4261 cgtcgagcgg ttcaaaaccg gcgatcacca ccggtcgctg gtattcctgc gcgaagaaat
     4321 cgtacggccg gctgccgatc acggtgctca catgcgacgg tccgatgaaa ccatccaccc
     4381 gcacgctgcc gagcttgcgc acgtcgggtg aatccagaat gctctggatg gctgagggcg
     4441 tcagcacatg attgcaaaac acgctgaaat ttttcaggcc cagcatttcc gcctgcttga
     4501 tggccacggc ggtcggtggc gtggtggtct cgaagccgat cgcgaaaaaa actacttcct
     4561 tgtccggatt gtcctgtgcg atctggatga cctcggtgca ggaatacacc atgcgcacgt
     4621 cgcggccctg cgcacgcgat ttcagcaggc tgcgcttgcg cgagccgggc acgcgcagca
     4681 tgtcgccata actgcaaaga atcacgtccg gcacctccgc cagctcgatc gccatgtcga
     4741 ggcggctgat cggcagcacg cataccgggc aaccgggccc atgcacgaga tgcacgtttg
     4801 gaggcagcag gtcctggaca ccgtaacgga agatggcgtg ggtgtgaccg ccgcaaaatt
     4861 ccatgagact gtactcgcgc gccgggtccg cctccgcggc cagctcggcg gcgagcgcgg
     4921 tggctacgcg ccggtcacgg aattcattga tgtatttcat gcggccgggt ttcgagatgg
     4981 cgggcgatct cgtcaaacaa ggcgagcgat ttctcggcct cgtcaacgtc cagccgcgta
     5041 atggcaaagc ccacgtgcac gatcacgtag tcgccgacct ccacgccgtc caccagcatc
     5101 agcgaaatgt cgttgcggac gccgccgact tccaccacgg cacggtcatt gtcgagcttc
     5161 tgcaccacct tcgccggaat cgccagacac atgtctcaag ccccctttct cgttcacgcc
     5221 cgcgggatga atatgcataa tctaaggcac atcatcgttg atccacaaat gccgactttg
     5281 acccatatca acattaccgc cccatggccc ggtctgaacc tgccgcaccg tgaggtatca
     5341 aagggaaagg ctcaaacaca aggcaacatt cttcggtata atcacgcgcc gcttgtcagg
     5401 gaatccgcgc gccgatcgct cccgccacag acgcccgaat ggacagctca tacatgacca
     5461 aattcaggaa tttcttcacg cgcaaacgcg tgcgtctttt attgctgtcg ctggcgctgc
     5521 tgctgtcggg gttcgcgctt tatctcgata tcaccgtgcg cacgcacttc gagggcaagc
     5581 gctgggccat gccggcgcgg gtctacgcgc ggccgctcga gctttatccg ggcatgaaat
     5641 tgcgcccgga acaactggcg atggagctag ccatgctgga ttatcacgcg gcaggcgatc
     5701 cgcagagccc cggcaattac cagcgccagg gcgatgagtt cactctggtc acacgtccgt
     5761 tcaccttctg ggacgaggcc caggccagcc tgccgttgca gatcgacttc gacggcgcgc
     5821 atttgggcac cctgaccaat cgctctaccg gcaaaccggt gacactggcg cggctcgatc
     5881 ccgtgctgat cggcggcatt tacccggcgc ataacgaaga ccgcgtgctg gtgaaattcg
     5941 acgagatacc accgcacctg gtcaaggccc tcatcgcgat cgaagaccgg caattctatt
     6001 cccatcacgg cttttccgtg cgtggcacgg cacgcgcact ggtgtcgata ttgagtggca
     6061 gtggcgtgca gggtggcagt accctgacgc agcagctcgt gaaaaatttc ttcctcacgc
     6121 cagaacgcac cctgcggcgc aagttcacgg aagtgatcat ggccatgctg ctcgagctgc
     6181 actattcgaa ggacgagatt ctggaagcct acgcgaacga gatctatctc ggccaggacg
     6241 gcaatcgcgc cattcacggc ttcggcctcg ccagccattt ttatttcgac cgcccgctgt
     6301 cgcgcctcga tctcccgcaa gcggcgctac tggtcggtct ggtcaaagga ccgtcctctt
     6361 acgacccgcg gcgccagccg gcgcgcgcgc tggcgcggcg caacctggtg ctgcaggaaa
     6421 tgcgcaagct ggattacatc acacaggcgc aattcgtcgc ggcccgcgcc gcgtcattgg
     6481 gtgtggcgga aaaagccccc gaagggacat cgctttatcc ggcgtttgtg caactggtgc
     6541 accgccagtt acggcgcgat tatcgcgaag aggatctgcg ttccgagggc ttgcagatat
     6601 tcaccacgct cgacccgctg gtacagacca gcgccgaacg cgccctcacc acccggctgg
     6661 cgcaactgga acgggaccgg cgcattccac cgaaggcgct ggagggcgcg atcgtggtca
     6721 gcaactcgca aaatggcgag gtgcaggcgc tggtgggtgg tcgcaataca cgattcgagg
     6781 ggttcaaccg cgcgctcgac gccgcgcgcc cgatcggctc gctgatcaaa ccggtggttt
     6841 atctgacggc gttggaacgc ccggcctcct atacgctcgc cacgctgctc gacgacagcc
     6901 cgctggtgtg gcgtcaacgc gggaccagcg actgggagcc gcagaactac gacaagcagt
     6961 ttcacgggca ggtgccacta cgagtagccc tggccaattc ctataatgtc tccacggcgc
     7021 gtctgggtct ggcgctgggg gtgcgtcacg tcgtggacag cgcgcatcgc ctcggcgtcg
     7081 agcgcgacat gaatgcctac gcctccaccc tgctgggtgc ggacagcctg agcccgctgg
     7141 aagtcaccca gatgtaccag tccttcgcca gcggcggctt ccgcgttcca ttgcgcgcga
     7201 ttcgcgaggt gctgatggcg gacggccagc cgctgcaacg ctacgcgctt tcagtggagc
     7261 aggtgatcga gccggggccg gcgtatctgg tgacccgcgc gctgcaaacg gtggtgcagg
     7321 aagggaccgc cgatgcgctt aaaaaatacc tgtcatccga tctgcatctg gccggcaaga
     7381 ccggcaccac caacgacttg cgcgacagct ggttcgcggg ttataccggt gatcgtctgg
     7441 cggtcgtgtg gataggtcgc gatgacaatc agccgatcaa catgaccggc gcgggcggcg
     7501 ccatgaccgt gtggggcgag atgatggcgc ggctggatcc cgatccgctc attccgccgc
     7561 tgccggagaa catcgagacg atatcaatcg atcccgtgag cggcctgcgc gcggacgcga
     7621 attgccagga ggccgtcgag ctgcctttca ttcgcggctc gggacccacg gagacagcgc
     7681 cctgcgcaac gcactcgccg gcgcgttcca tcaaaagctg gttcgagagg ttatttgaat
     7741 gagcaaacag cgttccatta ttttgttttt tatcgcggcg gcgtcagtgg cgggctgtgc
     7801 cggatttcct ccaacagcgc cggaggctca acccgtatcg gataacaccg ccgtggtggc
     7861 attaatggat acggcacgaa ccgacatcgc caatggaaag ctcgatgcgg cggtagcgcc
     7921 cctggagcgg gcgctgcgca tcgaaccgcg caatcccatg ttgtggcagg aactggccag
     7981 actgcgcttg caacaagggc agtatcagca ggccgagggc atggcaacgc gatcaaacag
     8041 ttgggcagga gacaacaaga ccttgcgcgc tgagaactgg cgcatcatcg gcgaggcgcg
     8101 cctgaagcgt ggtgaccatc agggcgccca agccgccttc gacaaggcgg ccacgcaaaa
     8161 ttgaatgccg gctttccgct caacggccag aaagttcatg acaacgttgt ttcgggttcc
     8221 ggccctgctc taaaatcaca ccatgattca gcgcggttgg ctgttcctgt tgtttctggt
     8281 tctgacgggt tgcgccacgc cggaagcgga gatactgagc aaagtcgaat cgttcccgcc
     8341 cacgctcaaa gttgaagtat tactggaccg ccccacacga ccgcacaaaa ctatcgccat
     8401 cctgggcgat gtctacggag gcactccgga ggaagtcaac gaacggctct cgcgcaaggc
     8461 gcgcgaaatc ggcgccgacg ccatcgtcat catgagtgtg cacgacaaat ccaccacgga
     8521 ttggctgctg gatgatccct tttattatcc ccatggcgtg cactggccgc gctaccgccc
     8581 tgtgcgatac acctatcgca tcgtccgcgc acgcgccatt aaatataccg aatgaccgcc
     8641 gaaaacctgt ggctgcaagg tggcggtctc gtcaccggtc tgattttcgg cggcatcgtg
     8701 cagcgtcagc gtttctgcat ggtggcggcc atcggcaatc tcgtattgat gcgcgactgg
     8761 cgacataccc acgcctttct cgccgccttc gccgtagcca tcgccggcac tcagtggctg
     8821 gaggcgagtg cgctggtgcc ggtggcggaa tccgcctacc gtaaggcgcg tttcgactgg
     8881 ctgggcgcga cggccggcgg tctgatattc ggt
//
LOCUS       DBNGZE010000243         4112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_867003, whole genome shotgun sequence.
ACCESSION   DBNGZE010000243 DBNGZE010000000
VERSION     DBNGZE010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_867003"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /gene="dsrK"
                     /locus_tag="AC16FV_06365"
     CDS             complement(<1..1037)
                     /gene="dsrK"
                     /locus_tag="AC16FV_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrK"
                     /protein_id="HIR2849715.1"
                     /translation="MADIKAPALGEYQVIPHVMRDTMAGSKPYVAKPDHNKSLGFPGE
                     LVPDWENVAVKKLGELVSQSRALQVFLDSCVKCGACTDKCHYYLGTSDPKNMPVARQD
                     LLRSVYRRHYTFAGKFFPKLVGARDMTKDVLDEWYSYFHQCSQCRRCSVFCPYGIDTA
                     EISMAAREIMDSVGQGQKYCNEILGKVHKIGNNLGLPGPALEDTLLGLEEDVKAEVGL
                     DVKYPLDVKGADVLLVTPSADFFAEPHIDGLIGYGKVFHAAGISWTLSTHASEAGNFG
                     MFVGSNANLHKVAKRIREAALELGVKRIIVGECGHAWRVAYSFWNTLIGPFDFLDRRY
                     PIPQHICELTY"
     gene            complement(1041..1787)
                     /locus_tag="AC16FV_06370"
     CDS             complement(1041..1787)
                     /locus_tag="AC16FV_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR2849716.1"
                     /translation="MSAVTVIYALLFYVATAILVIGVARKIFIFARTPQPLKIPTTPA
                     PTTRTGVWLRMAREVTVFESLFKANKWTWLFGWIFHFCLCVELIWHLRYFTEPVLFFL
                     PWLQWFINFTGYGMVLGLLGLWGRRFLVDRVRYISNPSDHLMLALLVGIGASGLSMKF
                     LFHTDIVSLKAFFLGLMTFNWQPLPADAPLLVHLTLVATLMIVFPVSKLLHAPGVFFS
                     PTRNQVDNPREKRWVAGWSAKIDNTKAAGG"
     gene            complement(1851..2183)
                     /locus_tag="AC16FV_06375"
     CDS             complement(1851..2183)
                     /locus_tag="AC16FV_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR2849717.1"
                     /translation="MPITVAGKTIETDEEGYLTNRTDWSEEIAKEMAKVDNCDLSQNH
                     WEVINFLREYYDEYQIAPAVRVLTKAIGKKLGPDKGNSKYLYELFPYGPAKQACKYAG
                     LPKPTGCV"
     gene            complement(2219..2518)
                     /gene="tusB"
                     /locus_tag="AC16FV_06380"
     CDS             complement(2219..2518)
                     /gene="tusB"
                     /locus_tag="AC16FV_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HIR2849718.1"
                     /translation="MLHTVNKSPTERNTLESCLKHLKKGSAVLLIEDGVYGALKGTAS
                     TKLVEQALKNHPIYALYPDIEARGMQDRVIDGIKLVDYSGFVDLAVEYPSVQSWL"
     gene            complement(2531..2938)
                     /gene="tusC"
                     /locus_tag="AC16FV_06385"
     CDS             complement(2531..2938)
                     /gene="tusC"
                     /locus_tag="AC16FV_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HIR2849719.1"
                     /translation="MTEATGGIEMEEDAGGKVKKFLYINRKAPYGTIYALESLEVVLI
                     AAAFDQDVSLAFLDDGVYQVVKGQHTKPIDVKNFSPTYRALEGYDIEKLYVEKESMEV
                     RGLKEDDLIVPVKVVNAQEMADIMQSQDVVLSF"
     gene            complement(2963..3322)
                     /gene="tusD"
                     /locus_tag="AC16FV_06390"
     CDS             complement(2963..3322)
                     /gene="tusD"
                     /locus_tag="AC16FV_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HIR2849720.1"
                     /translation="MKLGILINEGPYTHQASDTAYLFAKAAIEKGHKIHRVFFYHDGV
                     YNSSRLTEPPQDDRHIVNRWQKLAKDHHIDLVVCVAAALRRGIKDENLQEGFRISGLG
                     QLIETGIQSDRLVTFGD"
     gene            complement(3420..>4112)
                     /gene="dsrB"
                     /locus_tag="AC16FV_06395"
     CDS             complement(3420..>4112)
                     /gene="dsrB"
                     /locus_tag="AC16FV_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HIR2849721.1"
                     /translation="TQGWLHCDIPGTDASGVVKALMDDLHDEFVKEDMPNRVHITTSC
                     CQINCGGQGDIAINVQHTKPPKINHDLVANVCERPSVVARCPVAAIRPAMVNGKPSLE
                     VDEKKCICCGACFPPCPPMQINDPEHSKLAIWVGGKNSNARSKPMFHKLVAAGVPNNP
                     PRWPEAGAIVKKILRVYKQDAKDWERIGDWVERIGWPRFFELTELPFTKFHIDNWRGS
                     RHMLNQSAHIRF"
BASE COUNT          781 a         1196 c         1251 g          884 t
ORIGIN      
        1 tcgtaggtga gctcgcagat gtgctgcggt atggggtagc gccgatccag gaaatcgaac
       61 gggccgatga gcgtgttcca gaaactgtag gccacgcgcc aggcatggcc gcattcgcct
      121 actattatcc gcttgacgcc gagttcgagc gcggcttcgc ggatgcgctt ggcgaccttg
      181 tgcaggttgg cgttcgagcc gacgaacatg ccgaaattgc cagcctcgga cgcatgtgtc
      241 gagagcgtcc aggaaatgcc ggcggcgtgg aataccttgc cgtagccgat cagaccgtca
      301 atgtgcggtt cggcgaagaa gtcggccgag ggcgtgacca gcagcacatc ggcgcctttg
      361 acgtcgagcg ggtattttac gtccaagccg acttcggctt tgacgtcttc ttcaaggccg
      421 aggagcgtgt cttcgagcgc gggtcccggc aggccgaggt tattgccgat cttgtgaacc
      481 ttgccgagga tttcgttgca gtacttctgt ccctggccga cggagtccat gatttcgcgc
      541 gcggccatgg agatctcggc ggtgtcgatg ccgtaggggc agaacaccga gcagcggcgg
      601 cactgcgagc actgatggaa gtagctgtac cattcgtcga gcacgtcctt ggtcatgtcg
      661 cgcgcgccga cgagtttggg gaagaacttt ccggcgaagg tatagtgacg acggtaaacc
      721 gagcgcagca ggtcctggcg cgccaccggc atgttcttcg gatcggaggt gccgaggtag
      781 taatggcatt tgtcggtgca ggcgccgcac ttcacgcagg agtcgaggaa cacctggagc
      841 gcgcgcgatt gtgaaaccag ttcgcccagt ttcttgaccg cgacgttttc ccagtccggc
      901 accagttcgc ccgggaaacc cagcgacttg ttatggtcag gcttggcaac ataaggcttg
      961 gagccggcca tcgtatcccg catgacatgg gggatgacct gatattcgcc gagggcgggt
     1021 gctttgatgt cagccatgac tcagcctccg gcggccttgg tattgtcgat cttggcgctc
     1081 cagccggcga cccagcgctt ctcgcgcggg ttgtccacct ggttgcgcgt ggggctgaag
     1141 aacacgcctg gggcgtgtag cagcttgctg acggggaaca cgatcatcag cgtcgccacc
     1201 agcgtcagat gcaccagcag cggcgcgtcg gccggcagcg gctgccagtt gaaggtcatc
     1261 aatccgagaa agaacgcctt gaggctgacg atatcggtgt gaaacaggaa cttcatgctc
     1321 aggccgctgg cgccgatgcc gaccagcagc gccagcatca ggtgatcgga cgggttgctg
     1381 atgtaacgca cgcggtccac caggaagcgg cggccccata gtccaagcag tccgagcacc
     1441 atgccgtagc cggtgaagtt gatgaaccac tggagccagg gcaggaaaaa caggaccggc
     1501 tcggtgaagt agcgcaggtg ccagatcagt tccacgcaca ggcagaagtg gaagatccag
     1561 ccgaacagcc aggtccactt gttcgccttg aacaggcttt cgaacacggt gacttcgcgc
     1621 gccatgcgca gccacacgcc cgtgcgggtc gtcggtgccg gtgtggtcgg gattttcaac
     1681 ggctgcggcg tgcgcgcgaa gatgaaaatc ttgcgcgcga cgccgatgac gaggatagcc
     1741 gtcgccacgt agaacagcag cgcataaatc actgtcacag ccgacatcga tgaccccgct
     1801 ggtgcccggc aggggaggaa tccccgccga gggttgtcga gaatatttct ttagacgcag
     1861 ccggtcggct tcggcaggcc ggcgtatttg cacgcctgct tggccgggcc gtacgggaac
     1921 agttcgtaca ggtacttgct gttgcccttg tccgggccga gcttcttgcc gatggccttg
     1981 gtcagcacac gcacggcggg cgcgatctgg tattcgtcat aatattcacg caggaagttg
     2041 atcacttccc agtgattctg cgacaggtcg caattgtcga ccttggccat ttccttggcg
     2101 atttcctcgc tccagtcggt acggttggtg agatatcctt cctcatcggt ttcgatggtc
     2161 ttacccgcta cggtgatagg catgtgagta ctcctcgtcg gttaaagaat gaaaatattt
     2221 aaagccagga ttgcacgctg gggtattcca ccgccagatc cacaaacccg ctgtagtcga
     2281 ccagcttgat gccgtcgatg acccggtcct gcatcccgcg tgcttcgata tcgggataaa
     2341 gcgcatagat cggatgattc ttgagcgcct gctcgaccag cttggtgctc gcggtgccct
     2401 tgagggcgcc gtagacaccg tcttcgatca gcagcacggc gcttcccttc ttgagatgct
     2461 tgaggcagct ctcgagggtg ttacgttcgg tgggtgactt gttcacggta tgcagcatcg
     2521 gtcattccct ctaaaagctt aaaaccacgt cttgcgattg catgatgtcc gccatttcct
     2581 gggcattcac caccttcaca gggacgatga gatcgtcctc cttcaggcca cgcacctcca
     2641 tggattcctt ctccacgtaa agcttctcga tgtcataacc ttccagcgca cggtaggtcg
     2701 gtgaaaagtt tttgacgtcg atcggcttgg tgtgctgacc cttgacgacc tgatacacgc
     2761 cgtcatccag aaatgccaga ctaacgtcct gatcgaacgc cgccgcgatc aacacgactt
     2821 ccagtgattc cagcgcgtaa atggtcccgt agggggcctt gcggttgatg tacagaaact
     2881 tttttacctt gccgcctgcg tcttcttcca tctcaatgcc gccagttgct tcggtcattc
     2941 tgttcccctc gatagttccg ggttaatcgc caaatgtcac cagtcggtcc gactggatcc
     3001 ccgtttcaat taattgcccc aggccggaga tgcgaaaacc ttcctgaaga ttttcgtcct
     3061 tgatgccccg gcgcagcgcc gccgccacgc agaccaccag atcgatgtgg tgatccttcg
     3121 ccagtttctg ccagcggttg acgatatgac ggtcgtcctg cggcggctcg gtgagccggc
     3181 tcgagttgta gacgccatca tgatagaaga acacgcggtg aatcttgtgt cctttctcga
     3241 tcgcggcctt ggcgaacagg taagccgtgt ccgacgcctg atgcgtatac ggcccttcat
     3301 tgatcaaaat acccaacttc atcatcgctc cccttgatat ttatcaacaa cccccgacga
     3361 tttacgggcc gacgccggga aaaacccggc gtcggatacg gctactatgt gctggcgtac
     3421 tagaaacgaa tatgtgccga ctggttgagc atgtgacgcg agccgcgcca attgtcgatg
     3481 tggaacttgg tgaacggcag ttccgtcagt tcgaagaagc gcggccagcc gatgcgctcc
     3541 acccagtctc cgatgcgttc ccaatccttg gcgtcctgct tgtagaccct gaggatcttc
     3601 ttgacgatgg caccggcttc cggccagcgc ggcggattgt tcgggacgcc cgcggccacg
     3661 agcttgtgga acatcggctt ggaacgggcg ttcgaattct tgccgccgac ccagatcgcg
     3721 agcttggagt gctccggatc gttgatctgc atcggagggc agggcgggaa gcaggcgccg
     3781 cagcagatgc actttttctc gtccacttcg agcgagggct tgccgttgac catcgcgggg
     3841 cggatcgccg ccaccgggca gcgcgccacg accgatggac gctcacacac gttggccacg
     3901 agatcgtgat tgatcttcgg cggcttggtg tgctgcacgt tgatcgcgat gtcaccctga
     3961 ccgccgcagt tgatctggca gcaggaggtg gtgatgtgca cgcggttcgg catgtcttcc
     4021 ttcacgaact cgtcgtgcag gtcgtccatc agcgccttga ccacgcccga ggcgtcggtg
     4081 ccggggatgt cgcagtgcag ccagccctgg gt
//
LOCUS       DBNGZE010000244         4253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1561780, whole genome shotgun sequence.
ACCESSION   DBNGZE010000244 DBNGZE010000000
VERSION     DBNGZE010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1561780"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1414
                     /locus_tag="AC16FV_06400"
     CDS             410..1414
                     /locus_tag="AC16FV_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849722.1"
                     /translation="MMASSLRKTAVAVGVAGMLAATGTTQANVVNFSWRGAFTFLNYT
                     GQPMSNSPSDYVAGYYANGDSTAGTGGAPYFPANVAPGADCTTWSPACVTNYGWYGNR
                     TPVSGTLSFDTSTGAGVGTINPFFFWGDTPGSGPYTSVGQFQNVTFQVIDTIGTILGS
                     MLFSWNGLGHSVSIVLDGSGLFANMLTMITNGPTSTVSGVGALPASNGVDFDPSKNVK
                     TYPLGPIPVATKSLNTGAGCDGLALATQVNAYSIVTNLANVGTCTTGMVDDGVGGDPM
                     TSYAIPFNMNFDLTSVHLDYPWQCLSCPPPPVPVPPAAWLFGSGLLGLIGLARRRRGH
                     "
     gene            1536..2255
                     /locus_tag="AC16FV_06405"
     CDS             1536..2255
                     /locus_tag="AC16FV_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849723.1"
                     /translation="MTDESHITRLIQQAETCLDNGDLAQARRLYAEICRARDSDAQAW
                     LMYGAINGELGNAEVARNALERAVALEPGNAEAHLALAHLLRAGQKTVEAMNAALRAV
                     EADPEFVEGWLFVAAIAGQLADWPRAEEACHKAVALAPERTEGYINLGNLLLATGRLP
                     QAEENFRKALALGETAEAWFGLGTALGAQNHYAEAEPALTSALRLDSDSAVFREALAG
                     CQNQLGRSADSAAVRPDSSPN"
     gene            2345..>4253
                     /locus_tag="AC16FV_06410"
     CDS             2345..>4253
                     /locus_tag="AC16FV_06410"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR2849724.1"
                     /translation="MPTDTASANDDTNSRFTQALALHQAGRLDEAGILYEKILASDDR
                     HAGALGNLGFIHLQRGDRKECIRLLAKSLEVFPNQPAALNVLGMALQDLNRPEEALES
                     YDRALALRPDLAETHYNRGNTLQALNRLDEAVAGYDRALALRPEFFEAQNNRSIALER
                     LNRPEEALASCDRALAMRPDVVQPHNNRGNILKKLGRPDEALASYDRAIALRPDYVEA
                     HYNRGNLLQFFLHRPDEALVSYERAIELKPDFPYLLGTWMHCKMHCCDWTDLDAAWAR
                     VTSAIDRGETAAAPFAIPAIPASPAQQQRCARICIRDRFPSCAAPIARESHVSRDRIR
                     VGYFSSDFGDHPVSHLIAQLIESHDRARFEVIGFSLGPNPRDAWRERLERAFDRLVDV
                     SSRTDREVVGLARELEIDIAVDLNGFTKGARTGIFALRAAPVQVNYLGFPGTLGADYM
                     DYLIADATVIPPEHRQYYDEKIIHLPHCFQVNNTTREISDWPLRRAELGLPDDAFVFC
                     CFNHNYKITPDVFDIWMRLLRATDGSVLWLSGGNAAAARNLHREAEKRGVSRERMVFA
                     PRVEKLADHLARYRQADLFLDTFYYNAHTTASDALWAGLPVLTCLGGALSGRFAASVL
                     TAIGLQELIARS"
BASE COUNT          820 a         1357 c         1295 g          781 t
ORIGIN      
        1 aattggatga ccgttgtctg cggtttcatg aaacccgtcg cttcgtcggc acagccagtt
       61 acgaccaggt cagccggccg ctgtaccggc agtccattgc ccggtggaag aactatgaac
      121 ggcatctggg gccactgctg gccgagttga atcaataatt tattcaagaa acaacgcgac
      181 gagcgcgtga cgacgcttct cgcggactgc gatcatattg tgagtcgtcc cacgcgaatt
      241 attgccttcg ggtccggcag gcgtatgatg gtttgcacgg tgtccgttac atgccaaacg
      301 gttccgcagt tcaggccatc gcggcgcttc ataccggaat cgctcacgga gtgattttgg
      361 attctctccg tcgagccatc ataaaccaac aagaaacagg aagagaccaa tgatggcgtc
      421 atcactcagg aaaaccgccg tggcagtggg cgtggcggga atgctcgcag ccaccggcac
      481 gacgcaggcg aacgtggtga acttcagttg gcgcggggcg ttcacctttc tcaactacac
      541 aggccaaccg atgtcaaaca gcccctcgga ttacgtggcg ggctattacg ccaacggtga
      601 cagtaccgcg ggcactggcg gggccccgta cttcccggcc aatgtcgctc ccggtgcgga
      661 ctgcacgacc tggagccctg cttgcgtgac gaattatggc tggtacggca accgcacacc
      721 ggtgagcgga acgctgtcgt tcgacacctc gacgggtgcg ggcgtcggca cgatcaatcc
      781 gttcttcttc tggggggata cccccggcag cggtccgtac acctcggtcg gccagtttca
      841 aaacgtgacc ttccaggtca ttgataccat cggcaccatc ctgggcagca tgctgttctc
      901 ctggaacggt cttgggcact cggtgtccat cgtgctggat ggctccggcc tctttgccaa
      961 catgctgaca atgatcacca acggaccgac ctcgaccgtc tccggcgttg gcgcgctgcc
     1021 agcgtcaaac ggcgtggact tcgatccaag caagaacgtc aagacctacc cgctcggtcc
     1081 gattccggtg gccaccaagt cactcaacac cggtgcgggt tgcgatggcc ttgccctggc
     1141 cacgcaggtg aatgcctatt ccatcgtcac gaacctggcg aacgttggta cgtgcacgac
     1201 gggcatggtg gatgacggcg ttggcgggga tccgatgacc agttacgcca tcccattcaa
     1261 tatgaacttc gacctcactt cggtccatct ggattatcca tggcaatgcc tgagctgccc
     1321 gccaccgccg gtcccggttc caccggcggc ctggctgttc ggctcggggc tgctcgggtt
     1381 gatcggcttg gcgcgacgca gacggggcca ttgagagatg atcgccggcg tctgccctac
     1441 cggtccgaca aaaccgcggc tagaatgctg gtgcttcccg attctggcgg gaagatcaag
     1501 tatcgttcaa aaatcggcat acgggcctat ttatcatgac tgacgaatcg catatcaccc
     1561 gattaatcca gcaggcggag acctgtctcg acaatggcga cctggcgcag gcgcgccggc
     1621 tttatgccga gatctgccgc gcgcgggaca gcgacgcgca ggcttggctg atgtacggcg
     1681 ccatcaacgg ggagctcgga aacgcggagg tggcccgtaa cgccctagaa cgcgccgttg
     1741 cgctcgaacc cgggaacgcc gaggcgcatc tggcgctggc acatctactc agggccgggc
     1801 aaaaaaccgt ggaggccatg aatgccgcct tgcgggcggt cgaagccgac ccggaattcg
     1861 ttgagggatg gctgttcgtg gcggcgatcg ccggacaact ggcggactgg ccgcgagccg
     1921 aggaagcctg ccacaaggct gtcgcgctgg cgccggaacg cacggaagga tatatcaatc
     1981 tcggaaacct gctgctggcc accggacgtt tgccccaggc ggaagaaaac ttccgcaagg
     2041 cgctggcact tggcgagacc gccgaagcct ggttcggtct cggcaccgct ctgggtgcgc
     2101 agaaccacta tgccgaagcc gaacccgcgc tgacctccgc gctgcggctc gattcagaca
     2161 gtgcagtctt tcgcgaagcc ctggcgggct gccagaacca gttggggcgt tccgccgatt
     2221 ccgcggcagt gcggccggat tcatcgccca actagaaagc gagccggttt catcatcgct
     2281 cgcttgccgg agccggtcac gcgagcctcg attaccgacc atggaaaaag acggtgcatc
     2341 cgacatgccc acggacactg cgtcagcgaa tgatgacacg aacagccggt tcacccaggc
     2401 actggcgctg catcaggcgg gcaggctcga cgaggcggga atactgtatg agaaaatcct
     2461 cgcctctgac gacaggcatg ccggcgctct cggcaatctc ggcttcatcc atctgcaacg
     2521 cggcgatcgc aaagaatgca tccggttgct cgcgaaatcg ctggaagttt ttcccaacca
     2581 gccagcggcg ctcaatgtcc tgggcatggc gctccaggac ctgaaccgtc ccgaagaagc
     2641 cctcgagagc tacgaccgcg cgctggcgct gcggccggac ctggccgaaa cgcactacaa
     2701 ccgcggcaac acgctccagg cgctcaatcg gctcgacgag gccgtggccg gttacgatcg
     2761 cgccctcgct cttcggcccg aatttttcga ggcacaaaac aaccgcagta tcgcgctcga
     2821 gcgcctcaac cgccccgagg aagcgctggc cagttgcgac cgcgcgctcg ccatgcggcc
     2881 cgacgtggtg caaccgcaca ataaccgggg caatattctc aagaaacttg gacgcccgga
     2941 tgaagccctg gcgagctatg accgcgcgat cgccctgcgg cctgactacg ttgaagccca
     3001 ctacaatcgc ggaaacctcc tgcaattttt cctccaccgt ccggacgagg cgctggtcag
     3061 ctacgagcgc gccatcgaac tcaaaccgga tttcccatac ctccttggca catggatgca
     3121 ttgcaagatg cactgttgcg actggacgga tctggatgcg gcctgggcca gggtgaccag
     3181 cgcgattgac cggggcgaaa ccgcggcggc gccctttgcc attccggcca ttccggcaag
     3241 tcccgcccag cagcaacgct gcgcgcggat ctgcatccgg gaccgctttc cgtcgtgcgc
     3301 cgcccccatc gcgcgcgaaa gtcatgtttc ccgcgaccga atccgcgtcg ggtatttctc
     3361 cagcgatttc ggtgaccatc cggtatcgca cctgatagcg caattgatcg aaagccacga
     3421 ccgcgcccgg ttcgaggtca tcggcttttc gctggggccc aacccgcgcg acgcctggcg
     3481 cgagcgcctg gaacgggcct tcgaccgcct ggtggacgtt tcatcccgca ccgaccggga
     3541 ggtcgtcggg ctggcgcggg aactggagat cgacatcgcg gttgacctga acggattcac
     3601 caagggcgcg cgaaccggaa tttttgcgct gcgcgctgcg cccgtgcagg tgaattacct
     3661 gggctttccg ggaacgctcg gcgcggatta catggattac ctgatcgccg acgccacggt
     3721 gatccccccg gaacaccggc aatattacga cgaaaaaatc atccatcttc cgcattgctt
     3781 ccaggtgaac aatacgacgc gggaaatttc cgattggccc ttgcgccgcg ccgagctggg
     3841 ccttcccgac gatgccttcg tgttctgctg cttcaaccac aactacaaga tcacgccgga
     3901 cgtgttcgac atctggatgc ggctgctgcg cgcgaccgac ggcagcgtgc tctggctgtc
     3961 gggcgggaat gcggcggcgg cgcgcaacct gcatcgcgag gcggagaagc gcggcgtatc
     4021 ccgcgaacgg atggtatttg cgccgcgcgt ggaaaaactg gccgatcatc tcgcacgcta
     4081 ccgccaagcg gatctttttc tggatacgtt ctattacaac gcccacacca cggccagcga
     4141 tgcgctgtgg gcgggcttgc ccgtgctgac gtgcctgggc ggcgccttat cggggcgttt
     4201 tgccgcgagc gtgttgaccg cgatcgggct gcaggaattg atcgcgcgct ccc
//
LOCUS       DBNGZE010000245         5843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1214323, whole genome shotgun sequence.
ACCESSION   DBNGZE010000245 DBNGZE010000000
VERSION     DBNGZE010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1214323"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /gene="asnB"
                     /locus_tag="AC16FV_06415"
     CDS             complement(<1..1224)
                     /gene="asnB"
                     /locus_tag="AC16FV_06415"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR2849725.1"
                     /translation="MCGIAGLIGRVGEPNRAALRRMNEAIAHRGPDSEGFWEAAPDER
                     GWAPMLAHRRLSILDTTTSASQPMVDPETGHVVVFNGEIYNYVELRDKLVASGHVLQS
                     TGDTAAMLRTIGVYGRETIGDLRGMFAFALWDPRQKKLVVARDALGIKPLYMARNPDP
                     NGDWSLIFASEVRAILASGLLGKPRLNPRAVASVVWNGFTVAPETAVSGVESVWPGEC
                     RVFNARGTEEHREFFWRMPPGREDGTMDERRLAGVLEETVRLHLATDVPLGVFLSGGI
                     DSSAVANLAQKSSVSPVHTFTLAFEEQERNEGAFARRIAAAIGTQHRELVLTERDFMA
                     RLEGALDSLDQPTFDGLNSYFMSQAVRDAGFKVALVGSGGDELFGGYTSFRDLPVLHR
                     WLRRARHVPTGLRVAL"
     gene            complement(1698..2678)
                     /locus_tag="AC16FV_06420"
     CDS             complement(1698..2678)
                     /locus_tag="AC16FV_06420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019438661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2849726.1"
                     /translation="MTRVIGVVVIGRNEGERLRKCLASVQRVAGRVVYVDSGSTDDSV
                     AMALGMGLEVVELDLSTPFTAARARNAGFNRLLESAPGVSYVQFVDGDCEIIGGWLET
                     AAAFLNEHDDIVMACGRLRERYPERSVFNLLCDIEWDTPVGASKACGGIAMVRAGAFK
                     QVGGYRANLIAGEEPELCVRLRAAGWKVWRLDTEMALHDAAMTSLGQWWKRALRGGYA
                     FAEGSYLHGAPPERHCVKQSRSIRFWGGMVPLATFAAILAWGPWGLTLLAIYPLQVVR
                     LAMQSKRSSARENWWWAVFMVMGRFPEMLGQLKYWWNRISNKTNSLIEYK"
     gene            complement(2946..3941)
                     /locus_tag="AC16FV_06425"
     CDS             complement(2946..3941)
                     /locus_tag="AC16FV_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR2849727.1"
                     /translation="MNSALKAMNKFDYIMISAHGRSGTNWLLEVLDLSVHTYCRNEPN
                     ELKDSLLNALGSEWAIENDGAVLNDMWDKAISRTAISIGTRDHRITSDKDFLRALPKS
                     LGVYRLISSSRMRRLINKIEPAANCHEWLASPLVFKRNQMHEALTVIKINQAPGWACW
                     LLENRKHAGILHIVRHPAGMLNSWKKRYLNKNVYEKVRAESRDRLNKIASLAPEWRNR
                     FGDIDNMSAEESELWFWRYSTESVHLAGRSSERYHLVLFDDLACNPDKVAREVYQFCG
                     LDYTTDICNQVRSLSENSQSIASSWKKTISDQDRQYIERICSGGLISEWWPDVAS"
     gene            complement(4168..5247)
                     /locus_tag="AC16FV_06430"
     CDS             complement(4168..5247)
                     /locus_tag="AC16FV_06430"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2849728.1"
                     /translation="MSITYRLIAAHELDATLIDAWRSIQAKDHALSSPYFCPEFTQAV
                     SQVRDDVRVVVIEDAGRPVGFFPHQRSAFGRGGPVGGPLSDYHGVIAEPNSAWDLRAL
                     MRAAKLSVWTFDHLVGDIGKFDAYVTARATSPQINLTPGYQQYAQGCRDAGSDYIKKT
                     EGLARKLGREFGELHFTLHETKSGALDQLIRWKRDQYRGAKLTDVFGVPWTGKLLARI
                     AHARSAGFSGVCSVLRVGDRIVAAHLGMRCREVLHYWFPAYDSEFAKFSTGIILLLRM
                     AETLAGDGVRTIDLGKGDAQYKQRLMTGAVELREGAVELPSFLSSARQLQRMAEARAA
                     RGGLAAVMRLPLKAIRRIQWTRKFR"
     gene            complement(5249..>5843)
                     /locus_tag="AC16FV_06435"
     CDS             complement(5249..>5843)
                     /locus_tag="AC16FV_06435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2849729.1"
                     /translation="QHPTVGIVGPAIIEGDADRIGELQVTGERPTPAMLLQAALPLER
                     YPAFTHPIVPGSAPSRTGWVCGAALMIRTDVMKRLHGFDPRFFLYWEETDVCQRAEDM
                     GFETWALGTAVARHTGGASSSLNDTRIGGCIAKHYYQSRYYYMVKHHGRFAAVIAELG
                     EFLLLGMLSIVDVARGRGLHRLRSRMQAPLFSEPERM"
BASE COUNT         1240 a         1821 c         1555 g         1227 t
ORIGIN      
        1 cagcgccacg cgcagcccgg tgggcacatg ccgcgcgcgc cgcaaccaac gatgcagcac
       61 cgggagatcg cggaacgacg tgtagccgcc gaaaagctcg tcgccgccgc tgcccacgag
      121 agcgaccttg aagccggcgt cacgcactgc ctgcgacatg aagtacgaat tcaatccgtc
      181 gaaagtcggc tgatcgaggc tatccagcgc tccttcgagc ctggccatga aatcccgctc
      241 ggtcaacacc agttcccggt gttgcgttcc aatcgcggcc gcgatccggc gtgcgaaggc
      301 gccttcgttg cgctcctgtt cctcgaaggc gagcgtgaag gtgtgtaccg gcgagacgga
      361 gcttttctgg gccaagttgg ccaccgccga cgagtcgatg ccgcccgaca ggaacacccc
      421 caggggaacg tccgtggcca agtgcaaccg gacggtttcc tcgagcacgc cggcgagtcg
      481 acgttcgtcc atcgtaccgt cttcgcggcc gggcggcatg cgccagaaaa attcgcggtg
      541 ctcctcggtg ccgcgcgcat tgaaaacgcg gcattcgccg ggccagacgg actcgacgcc
      601 gctgacggcg gtttcgggcg cgacggtgaa accgttccag acgaccgatg ccacggcgcg
      661 cgggttcagc cgcggtttgc ccagtagtcc gctcgcgagg atggcgcgaa cctcggaggc
      721 gaagatgagc gaccagtcgc cgttcggatc gggattgcgc gccatgtaca gcggcttgat
      781 gccgagcgcg tcacgggcca ctacaagttt tttctgccga gggtcccaca acgcgaacgc
      841 gaacatgccg cgcagatcgc cgatggtttc gcgcccatac acgccgatcg tgcgcaacat
      901 ggcggcggtg tcgccggtcg actgcaggac gtgtccggag gcgacgagct tgtcgcgcaa
      961 ctcgacgtag ttgtagatct cgccattgaa cacgacgacg tgcccggtct ccgggtcgac
     1021 catgggctgc gaggccgacg tcgtggtatc gagaatagac agccgccggt gcgccagcat
     1081 cggcgcccaa ccgcgttcat cgggcgcagc ctcccagaag ccttcgctat ccggcccgcg
     1141 atgcgcgatg gcctcgttca tgcgccgcaa tgcggcgcgg ttgggttcgc ctacgcgacc
     1201 gatcagtccg gcaatgccac acatggttaa tggttcttca atgcagaact ttgggatgtg
     1261 gaaaggcggc acggacgggc tgcccggcgc cgcgccacgt gatcgtaccc gccccgacag
     1321 tatcggtatc gaaaatcacg gacaaacagc cggcagaacc acgtcgtatg tcgcgcgctc
     1381 gcgagtatgg gcacggaagt ataaagaccc ttttttctga aagccatcga ccattccccg
     1441 gattcaggcg cccgtgatca cgccggtcgc ggacccggac ccgtcggttc cgtgtttcga
     1501 catgtgaaac cgcggttcgc ttttttttaa cttgccgccc cccggagccc cggcccgcgc
     1561 ccgggtccgt gaaagcggtc cggatcgccg gacatgatca aaccgcggat tcctcgaggc
     1621 gggcctgccg tgcgtgtcgg tgtaacgcaa ccgccgctga aaccggatgc gccggtgatt
     1681 aacaccttca tgggatgtta cttgtactca atgagtgagt tggttttgtt actgatgcgg
     1741 ttccaccaat acttcagctg ccccagcatt tccggaaacc tccccatgac cataaatacc
     1801 gcccaccacc agttttcccg ggcggaagac cgcttgcttt gcatagccaa acgaaccacc
     1861 tgcaaagggt aaatggccag caaggttaac ccccatggcc cccacgctaa tatggcagcg
     1921 aacgtggcca agggaaccat gccgccccag aaccgtatgc ttcgtgattg cttgacgcaa
     1981 tgccgttccg gcggagcacc gtgcagatac gacccctcgg caaacgcata gcctccgcgc
     2041 agcgcccgtt tccaccattg tccaaggctt gtcatggcgg catcatgcaa cgccatttcc
     2101 gtgtcgagcc gccaaacctt ccatccagct gcacgcaggc gaacacacag ttccggttcc
     2161 tctccagcga tcagattcgc gcgatacccc cctacctgct tgaatgctcc tgcccgtacc
     2221 atggcaatac caccgcaggc tttcgatgca cctacaggcg tatcccactc aatatcgcag
     2281 agcaggttaa agactgatcg ctccggatac cgctcccgga gtcggccgca tgccattacg
     2341 atgtcatcat gctcgttcag gaatgcagcg gccgtttcca gccagccacc gattatctcg
     2401 caatccccgt ccacaaactg gacataggac acgccaggcg cggattccag cagcctgttg
     2461 aaccctgcat ttcgtgcccg agccgcggta aaaggcgtgc tcagatccag ctcaacgact
     2521 tccaggccca tgccgagggc catggcaacg gagtcgtccg tcgaacccga atcgacataa
     2581 accacacgac cggccacccg ctgaacagac gccagacatt tccgcagacg ctcaccctca
     2641 ttgcgcccga tgaccacgac accaatcacg cgcgtcatgt accttcgggt cttgcggcgt
     2701 gtagtattgt tgcgaccatc ccgcggctgc aatgcgccac agcggctcga cgccggggtc
     2761 cgctgccgcc ggctgtcacg ggctcttcgt cagtgcttgg ctttccctgg ttcattgacg
     2821 ggctgtagat acggttatta attcacacac aatacgaaat ctctcggcgg gcatatgtgt
     2881 gattcaattg ttttttattt atcgtctttg acctggtgcc gggtaaaatt caacaataat
     2941 gatgttcatg aggcgacatc gggccaccac tcggatatca ggccgccgct acagattctt
     3001 tctatatatt gtctatcctg atcagatatt gtttttttcc atgaactggc gattgactga
     3061 gaattttcac tcaagctcct tacctgattg catatatcag tcgtatagtc caagccgcag
     3121 aattgataaa cctcgcgagc taccttgtcg ggattacagg ccagatcgtc gaataaaacc
     3181 agatgatatc tttcgctaga ccgtccagcc aggtgaacag attccgtgga ataacgccag
     3241 aaccatagct cgctttcctc ggcgctcatg ttgtcgatat cgccaaatct gtttcgccat
     3301 tcaggcgcca aactcgctat cttgttcagc ctgtcacgac tttccgcccg cactttttcg
     3361 taaacatttt tattcaaata tcgctttttc cacgaattca gcattccggc cggatgccgg
     3421 acaatatgca gaattcccgc atgcttgcga ttttcaagca accagcaggc ccaacctggc
     3481 gcctgattga ttttaataac agtcaatgct tcatgcattt ggttgcgctt gaacaccaga
     3541 ggtgatgcca gccattcatg gcaatttgcc gccggctcta ttttgttgat taatcgacgc
     3601 attcgagaac tgcttatcag acgatagacg cccaatgatt tgggaagtgc ccgcaggaag
     3661 tctttatcgc ttgttatcct gtgatcccgg gttcctattg aaatagctgt tctggatatc
     3721 gctttatccc acatgtcatt aagcacggcc ccgtcatttt ctatggccca ctccgagcca
     3781 agcgcattca acagggaatc cttcaattca ttaggttcat tacgacagta agtatgcacg
     3841 ctcaggtcta acacttcgag cagccagttg gtgcccgaac gaccatgcgc actaatcatt
     3901 atgtaatcga atttattcat ggctttgagt gctgagttca tggcttgggt tgcatggaat
     3961 aatgtcaggg ggggcaataa tcaatactac tatatcaagt cagcagcggc caaacctgga
     4021 atatgcccgt atcatatcca tagcaagatt caataaacat ttcttcgaat tacaaaatta
     4081 ttttggttat gaccggtaat gttcgggcag cggccttgcc tgccgccgac aaaaaacctg
     4141 cgtacgatcc ccagagatcc tgtctgctca gcgaaacttc cgagtccatt gaattcgccg
     4201 gattgccttg agcggcaatc gcatcaccgc cgccagtcca ccccgcgccg cgcgcgcctc
     4261 ggccatcctc tgcagttgcc gcgcactcga caggaacgaa ggcagctcga cggcgccttc
     4321 gcgcaattca acggcgccgg tcatgaggcg ttgcttgtac tgcgcatctc ccttgccaag
     4381 gtcaatcgtt cgcacaccat cgcctgccag cgtctccgcc attcgcagga ggagaataat
     4441 tccggtggag aatttcgcaa actcagagtc gtaggcggga aaccaatagt ggagtacttc
     4501 acggcaccgc atccccagat gtgcggcgac gatccgatca ccaacgcgaa gcacggaaca
     4561 cacgccggag aacccggctg accgtgcatg tgcgatccta gcgagcagtt ttccggtcca
     4621 aggcacgccg aaaacatccg tcagttttgc tccacgatac tggtctcgtt tccaacggat
     4681 gagttgatcc aacgctccgc tcttcgtctc atggagtgta aaatgaagtt ccccgaattc
     4741 acggcccagt tttcgcgcca acccctcggt ttttttgatg tagtcggagc cggcgtcgcg
     4801 acacccctgt gcgtattgct ggtagcccgg ggtcaggttg atctgcggag aggtcgcgcg
     4861 agccgtcaca taggcatcga actttccgat gtcgccaacg agatgatcga aggtccacac
     4921 agacaatttc gccgcgcgca tgagggcgcg taaatcccag gcgctgttgg gttccgcgat
     4981 tactccgtgg tagtcggaca ggggcccgcc aacaggcccc ccccttccga aggctgagcg
     5041 ctgatgtgga aaaaacccca ccggccggcc ggcatcctcg atcaccacca ccctaacgtc
     5101 atcgcgcacc tgactcacgg cctgtgtgaa ttccgggcaa aagtacggac tcgacaaggc
     5161 atggtccttg gcctgaatcg aacgccaagc gtcgatcagc gttgcgtcca gttcgtgagc
     5221 agcgataagc cggtaggtga tgctcacgtc acatgcgctc gggttcgctg aacaacggtg
     5281 cttgcatccg cgagcggagc cgatgcagtc cacgcccgcg agcaacgtcc acgatcgaaa
     5341 gcatgcccaa caacaggaac tcgccgagtt ccgcgataac tgccgcgaac ctgccgtgat
     5401 gctttaccat gtagtagtag cggctttggt aatagtgctt tgcgatacac cccccgattc
     5461 gggtgtcatt caacgaagag ctcgcgcccc ctgtgtggcg cgcgaccgcg gttccgagcg
     5521 cccaggtctc aaatcccatg tcttccgcac gctggcaaac atccgtttcc tcccaataca
     5581 ggaaaaagcg cggatcaaat ccatgcaaac gtttcatcac atccgttctt atcatcaggg
     5641 ccgcaccaca cacccaaccc gtccgggagg gcgccgagcc tggcacgatc gggtgagtga
     5701 acgcgggata ccgttccaaa ggtaaggcag cctgcaacag cattgccggc gtcgggcgtt
     5761 cacccgtcac ttgcagctcc ccgattctgt ccgcgtctcc ctcaatgatt gccgggccaa
     5821 caattccgac tgttgggtgt tgt
//
LOCUS       DBNGZE010000246         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1214331, whole genome shotgun sequence.
ACCESSION   DBNGZE010000246 DBNGZE010000000
VERSION     DBNGZE010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1214331"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1047)
                     /locus_tag="AC16FV_06440"
     CDS             complement(157..1047)
                     /locus_tag="AC16FV_06440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2849730.1"
                     /translation="MNKIGQRPLVVEQMSLRAELIRLCLPWFTRPRFPRDIQIEDVRR
                     RVARFGHFVPHPPRATEVIAIDAGGVKAERIATPRSRPNRYVLHLHGGAYLFGFPALF
                     RDLTWRIADAAGARVVCVDYRLAPEHPFQAAIEDATAAYRWLIAECAEPRHVAFIGDS
                     SGGGLALASMMRLRDEGSPLPAAAVVLSPWTDLALTGQYGFSDPMVPVELMPRAVELY
                     LAGADPRSPYVSPLYGDPAGLPPTLIHVGSDEALRDDAVRMAERLRVVGREVELEVWP
                     RMFHVWHMFARHGARLRGDI"
     gene            1301..2212
                     /gene="uppS"
                     /locus_tag="AC16FV_06445"
     CDS             1301..2212
                     /gene="uppS"
                     /locus_tag="AC16FV_06445"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR2849731.1"
                     /translation="MNSGLQPAERDAAALGLDPATRSALVDIDHAALGRRLRDKRAAP
                     SQLLRFVYWLYERRLLHQLERGSLPRHVGIILDGNRRHARRRGLGDPREIYQYGANKL
                     DDILDWCAELGISAVTLWVFSTENLKRPPAEVSGILAAIEAKLTALAHDPFMHQKRIR
                     VRAIGRLDILPESVVAAIRAAEVATAQYDSTTLTIAAAYGGREEIVDAVCSLLEAQAK
                     QGASLSDVIENMTSDAIARHLYAADLPDPDLIIRTSGEIRLSGFLLWQSVHSEFYFTD
                     VLWPAFRKIDFLRAIRAYQGRNRRFGH"
BASE COUNT          521 a          793 c          725 g          462 t
ORIGIN      
        1 cgcaacgcaa cgcgtcaagc gaacttgccc aatccgcgca gcggatgcgt ccgcggcgct
       61 tcgccacttg caacgccacg gggccatttt tatctgacac gaccacgcga ttcgccggtg
      121 cataaaattc agtttttggc acagcagcgc ccacactcag atatcgccac gcagtctggc
      181 cccatgccgc gcgaacatat gccagacatg gaacatgcgc ggccatactt cgagctcgac
      241 ctcgcggccc acgacgcgca ggcgctcggc catgcgcacc gcatcatcgc gcagtgcctc
      301 atcgctgcca acatgaatga gagtcggcgg caggcccgcc ggatcgccgt aaagcggaga
      361 gacgtagggc gagcgcggat cagcgccggc gagataaagc tctaccgccc tcggcatcaa
      421 ctcaaccggc accatcggat cggagaagcc gtattggccg gtcagcgcga gatcggtcca
      481 cggcgagagc acgacggcag cggccggcaa gggtgagccc tcatcgcgta gacgcatcat
      541 actcgcgagg gcaagcccgc cgccggacga atccccgatg aacgccacgt ggcgcggctc
      601 ggcgcactcg gcgatgagcc agcgataggc ggctgtcgca tcctcgatcg ccgcctgaaa
      661 gggatgctct ggcgcaagcc ggtagtcgac gcacaccacc cgcgcgcccg cggcgtcggc
      721 gatacgccag gtcaaatcgc gaaacagcgc cggaaagccg aacagatagg cgccgccgtg
      781 caggtgcagg acgtatcggt tgggacgcga gcggggcgtg gcgatacgct cggctttgac
      841 accgccagcg tcgattgcga tcacctcggt tgcgcgcggg gggtgcggca cgaaatggcc
      901 gaagcgcgcc acccgtcggc ggacatcctc gatttgaatg tcccttggaa accgtggccg
      961 cgtgaaccag ggcaggcaca ggcggataag ctcggcgcgc agactcattt gctcaaccac
     1021 cagaggacgt tgcccaatct tgttcacgcg ccgtaggccg cacccgtggc tccaaaacca
     1081 atcaggtttc tgtcttagca tcgggttacc cttggtatta aaccttcatt ccgaggctaa
     1141 accgcccttc atgctggcgc cctttaaatg tcgaaaatcg cgcgcagtac gctaccttgc
     1201 gtatgctttt tagaatgcac ccgggagccg ccgctttaag cgtaaactgg ccgatatatt
     1261 ccggttgacg caaaggggag agaaaaaggg ggggaagaag atgaattcgg gcctgcaacc
     1321 ggccgaacga gatgccgctg cgcttggcct cgacccagca acccgctccg ccttggtcga
     1381 tatcgaccac gcggctttag gtcgaaggct ccgtgacaaa cgtgccgccc cgtcgcaact
     1441 gctgcgattt gtctattggc tttacgagcg ccggctcctg caccagctcg aacgtggctc
     1501 gctgccccgc cacgtcggca tcatcctgga tggaaatcgc cgccacgctc gccggcgagg
     1561 cctcggcgac ccgcgtgaga tttatcaata tggggccaac aagctcgacg acatcctgga
     1621 ctggtgtgcg gagctgggca tcagcgcagt aacactctgg gtcttttcaa cggagaacct
     1681 taagcgacca ccggctgagg tttccggaat cctcgctgca atcgaagcca agcttaccgc
     1741 gctggctcac gacccgttca tgcatcaaaa acgcatccgc gtacgggcca tcggccgact
     1801 cgatatttta ccggaatcag ttgttgctgc gatccgcgca gcggaagtgg cgactgcaca
     1861 gtacgattca acgaccttaa cgattgcagc cgcatacggc ggccgggagg agatcgtcga
     1921 cgctgtctgc agccttctcg aagcgcaagc gaagcaagga gcatcattgt ctgatgtcat
     1981 agaaaacatg acatccgatg ctatcgcccg gcatctttat gcagccgatt tgccggaccc
     2041 cgacctgatc attcgaacga gtggtgagat ccgtttgtcc ggctttttgc tctggcagag
     2101 cgtgcacagc gagttttact ttaccgatgt cttgtggccg gccttccgca aaatagactt
     2161 cttacgtgcc attcgcgcct atcagggacg caaccgccgc tttggccact aattctcgtg
     2221 tcgcatcgca gaaacgcgcg cgatgattat ggaagcatca tgaccagttt gatgtgaatc
     2281 acttatggcg tcgaaatgag accgctgctg gtcgcgacta tcaaacggcc gccccaacgc
     2341 accatcgatt cgacccgtcc cactcccggc accgtgtctc cgcgcatcgc cttccagagg
     2401 ccattaggcc cttccaagac cgccgtgcca ttagtaatgc cacgaagagt ccagcctgga
     2461 attgttgtcg gtggtgtctc cggccacgga gtgagtttct c
//
LOCUS       DBNGZE010000247         2793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_728400, whole genome shotgun sequence.
ACCESSION   DBNGZE010000247 DBNGZE010000000
VERSION     DBNGZE010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_728400"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16FV_06450"
     CDS             complement(<1..185)
                     /locus_tag="AC16FV_06450"
                     /inference="COORDINATES: protein motif:HMM:NF021100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HIR2849732.1"
                     /translation="MYWQEAKENERYIVPDDIVDVVFSIQCRCLPVDHSYSLSQAISA
                     ALPWFADEEHAGLHTIHV"
     gene            complement(197..904)
                     /locus_tag="AC16FV_06455"
     CDS             complement(197..904)
                     /locus_tag="AC16FV_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849733.1"
                     /translation="MKYLKDESFKMDMDSAEDIQESTVLLEAQLAEFRRQMLELPPNY
                     DPLARADLLLQIGRTLIRLEKNQDAWVAGREAFDIFTAREAWEGAVQACDIMFLSDQP
                     ESLAALGQGIWLTVTFPMDPELSVAMLQHVVDETPSDSDGAAVAATMAHYLVDLRAEG
                     RQREDLMFYTNQLLATVARRHSDVKDQEAFGRWFKKLELEDPAKFLPRLRNIVDVLVQ
                     DEWWIDRDAIREKLPVN"
     gene            complement(956..1153)
                     /locus_tag="AC16FV_06460"
     CDS             complement(956..1153)
                     /locus_tag="AC16FV_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur relay protein DsrC"
                     /protein_id="HIR2849734.1"
                     /translation="MLHLSEVLLQHHDIQTFEQLLEVVRSRAQTEMFFRIDIKPPYPD
                     TPANWEDQLEGAFVGIHSVTR"
     gene            complement(1158..2102)
                     /locus_tag="AC16FV_06465"
     CDS             complement(1158..2102)
                     /locus_tag="AC16FV_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR2849735.1"
                     /translation="MNNSAQTAQTSRAMGSSPFYVENETAYQRWRMAKLENYPAAAEQ
                     LVVPVDNPMALTDAEAAAMLNVCRKTNMVIYASRARGEDKEIPRAVGERFGLRRLDSN
                     MLADDDGITSLQVVPGKSQRGYIPYSNKRLLWHTDGYYNSADNRIRAFVLHCVSPAAE
                     GGENSLLDHEIVYILMRDANPDYIKALMAPDAMTIPANTEAGVETRAAVSGPVFSVDP
                     VGGNLHMRYTARTRSIEWKQDTATRAAVQFLENLLAGESPYMFRHTMTAGQGLMCNNV
                     LHNRTAFTDDADRGIARLVYRARYYDRIRGTNFNETGH"
     gene            2471..>2793
                     /locus_tag="AC16FV_06470"
     CDS             2471..>2793
                     /locus_tag="AC16FV_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849736.1"
                     /translation="MAQNQPKNPEKHSTFIPKEKKSTKPFHKTPLLDQLESGPWPSFV
                     TGLKRLAHDNNMMVDLLGQLEHSYTTRKGYWKGGTVSVFGYGGGIIPRFTELMGSDGK
                     PMFPEA"
BASE COUNT          571 a          834 c          777 g          611 t
ORIGIN      
        1 acgtggatgg tatgcagacc ggcatgttcc tcgtccgcaa accagggaag cgccgcggaa
       61 atggcctgcg acaaggaata ggaatggtcc accggcaggc aacggcactg gatgctgaac
      121 accacatcca cgatatcgtc cgggacgata tagcgctcat tctctttggc ttcttgccag
      181 tacatatcct tcgccgtcag ttgaccggaa gtttctctcg tatggcgtcg cggtcgatcc
      241 accattcgtc ctgcaccagc acgtccacga tgttgcgcag gcgcggcagg aatttcgccg
      301 ggtcctcgag ctcgagcttt ttgaaccagc ggccgaaggc ttcctgatcc ttcacgtcgc
      361 tgtgccgccg cgccaccgtg gccaggagct gattggtata gaacatcagg tcttcacgct
      421 gacgcccttc ggcgcgcagg tcgaccagat agtgcgccat cgtcgccgcc accgcggcgc
      481 cgtcggaatc cgacggggtt tcgtccacca catgctgcaa catcgccacc gacagctcgg
      541 ggtccatggg aaaggtaacc gtgagccaga tgccctgccc gagggccgcg agcgattctg
      601 gctgatccga caggaacatg atgtcgcagg cctgcaccgc gccctcccag gcctcgcgtg
      661 ccgtgaaaat atcgaaggcc tcgcgaccgg cgacccaagc gtcctgattt ttctccagcc
      721 gtattagcgt gcgcccgatt tgcagcagca ggtcggcacg agcgagcgga tcgtaattgg
      781 gcggcagctc cagcatctgg cgcctgaact ccgccagctg ggcctcgagc aggacggtgg
      841 attcctggat gtcttccgcg ctatccatgt ccatcttgaa gctttcgtct ttgagatatt
      901 tcatgagttg actcttaaat cttggcggcc ttggcgtctt ggcggttcaa aaatattacc
      961 tggtcaccga atggatgccg acgaaggcac cttcgagctg gtcttcccag tttgccggtg
     1021 tgtccggata cggcggcttg atgtcgatgc gaaaaaacat ttccgtctgc gcgcggctcc
     1081 gcaccacttc gagcagctgt tcgaacgtct ggatgtcgtg atgctgaagc agcacttcgc
     1141 tcaaatgcaa catgatgtca gtgacctgtc tcgttaaagt ttgtcccacg gatgcggtca
     1201 taataacgcg cccggtatac cagcctggcg attccccgat ctgcatcgtc cgtgaacgcg
     1261 gtgcggttgt gcagcacatt gttgcacatt aggccctgtc ccgccgtcat cgtgtgacgg
     1321 aacatatacg gcgattcacc agccagcagg ttttccagga actgcacggc ggcgcgcgta
     1381 gccgtgtcct gtttccattc gatggagcgc gtgcgcgcgg tatagcgcat gtgcagattc
     1441 ccgccgactg gatcgacgga aaataccggc ccgctcaccg ccgcgcgtgt ttccacgccg
     1501 gcttccgtgt tggccggaat ggtcatggcg tccggcgcca tcagcgcctt gatgtaatcc
     1561 ggattcgcat cgcgcatcag aatgtaaacg atctcatgat ccagcagact gttttcgccg
     1621 ccttccgccg ccggcgacac gcaatgcaac acgaacgcgc ggatacggtt atcggcgcta
     1681 ttgtaatagc cgtccgtgtg ccacagcagg cgcttattgg aatacggaat gtaaccgcgc
     1741 tgcgacttcc ccggcactac ttgcaacgaa gtgatgccat catcgtccgc cagcatgttg
     1801 ctgtcgagac ggcgcagacc gaaacgctcg cccacggcgc gcggaatttc cttatcctcc
     1861 cctctggcgc ggctcgcgta aatcaccatg ttggttttgc ggcagacatt cagcatcgcc
     1921 gcggcctcgg catccgtgag cgccatggga ttatccaccg gtaccaccag ttgttccgcc
     1981 gccgcaggat aattctccag tttggccatg cgccagcgct ggtaagccgt ttcgttctcg
     2041 acataaaacg ggctgcttcc catggctcga ctggtttgtg ccgtctgcgc tgaattattc
     2101 atttcaaaat accccggaat gtttgtcaga taatggattc ccgggctagg tttccaagag
     2161 gcaaccgcgt tttaaaagac agcaacggcg cgggttttaa cgggacgata gtattttaca
     2221 aatcagatgg ttgtataata aacgctttct tgtggcggtc gcacgcttcc attcatatcc
     2281 ctttcgggag gggtaaaccc gggcgggctc tctctaacgt gtgattttct tattaagggc
     2341 caggtttaca ctgcccctcc gcaagcattc agttgcaagt ggtttcaacg gcagaaattt
     2401 ggacctttcg gcgtggaaca agcgctggaa ggcggaatgt acatccttcg acgcgcagga
     2461 ggtagtttaa atggcccaga atcaaccgaa gaacccggaa aaacattcca cgtttattcc
     2521 gaaagaaaag aaatccacca agccgttcca caaaactccg ctgctggatc agctggagag
     2581 cggcccgtgg cccagcttcg tgaccggact gaagcgcctg gcacacgaca acaacatgat
     2641 ggtggacctg ctcggtcagc tggaacactc ctataccacc cgcaagggct actggaaagg
     2701 cggcacggtc tccgtgttcg gatacggtgg cggcatcatc ccccgattca ccgaactcat
     2761 gggcagcgat ggcaagccca tgttccccga agc
//
LOCUS       DBNGZE010000248         2357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_867286, whole genome shotgun sequence.
ACCESSION   DBNGZE010000248 DBNGZE010000000
VERSION     DBNGZE010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_867286"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16FV_06475"
     CDS             complement(<1..250)
                     /locus_tag="AC16FV_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR2849737.1"
                     /translation="MKGIGYAFILVSLIAATACAGRRADVVIDPAGVDMGRYQQDLTE
                     CQEISKQVRGKAAGGAIGGAVVGGAVGGIVGNSDTAGKG"
     gene            complement(364..981)
                     /gene="msrA"
                     /locus_tag="AC16FV_06480"
     CDS             complement(364..981)
                     /gene="msrA"
                     /locus_tag="AC16FV_06480"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR2849738.1"
                     /translation="MNISRFVAVLGGAVLGMTLAATAAEPAAKGKTATATAIFAGGCF
                     WCMEPPFDALPGVSATTSGYTGGQKVNPSYEQVSAGDTGHVEAVQIAYDPKQISYEKL
                     LEVFWRNVDPLDSGGQFCDRGSTYTTAIFYANEEQKKLAEQSKTAIEKKLGKTVVTTI
                     RPATTFYAAEDYHQDYYKKNPLRYKYYRYSCGRDQRLEQLWGKKG"
     gene            complement(1147..1710)
                     /locus_tag="AC16FV_06485"
     CDS             complement(1147..1710)
                     /locus_tag="AC16FV_06485"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR2849739.1"
                     /translation="MSLSALHYSCLALGLILVSSPVLAGEFDVTVPMHDKGRATYYVN
                     AQVADLGASEFMVDTGSGYVTINEQTLNALQERKQAQYVKELRAILANGSELVIPVYA
                     INQLRIGGCTIHNVEAAVFPARTRQILGLSALNKAAPFTFSVDPPELVLSNCARNANI
                     PLAQPALAEATGPVVPATVGNTAPLRP"
     gene            complement(1943..2188)
                     /locus_tag="AC16FV_06490"
     CDS             complement(1943..2188)
                     /locus_tag="AC16FV_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR2849740.1"
                     /translation="MIKKHTLTALALMTVSGLAWAGSGAASFQDLDANRDGQITLDEA
                     KKSPEVKAKFTQADVNKDGKLDAAEFAALETMPKETK"
BASE COUNT          445 a          667 c          700 g          545 t
ORIGIN      
        1 cgcccttgcc ggcggtgtcg gaattgccca cgatgccgcc gactgcgcca ccgacgacgg
       61 ctccgccaat cgcgcctccc gccgccttgc cgcgcacctg tttcgagatt tcctggcact
      121 ccgtcagatc ctgctggtac ctgcccatgt ctaccccggc gggatcgatc acgacgtcgg
      181 cgcggcgccc ggcacatgcg gtggcggcga taaggctcac gagaatgaag gcatatccga
      241 ttcctttcac gacaggctcc cttttggatg gtctttacat gaatgtagga caccggccga
      301 atggattcca ggaatgtgtg gcacgttccg taaccttgtg tgtgtccgca ggttattcag
      361 gcgtcagcct ttcttgcccc agagctgttc gaggcgctga tcgcgcccgc agctgtagcg
      421 gtaatatttg tagcgcaggg gattcttttt gtagtaatcc tgatgataat cctcggcggc
      481 gtagaaggtc gtggccgggc ggatcgtggt gacgacggtc tttccaagtt tcttttcaat
      541 cgcggttttt gactgctccg cgagtttttt ctgttcctcg ttcgcataga aaatcgcggt
      601 ggtgtaggtg ctgccgcggt cgcagaactg tccgccgctg tccagcggat cgacattgcg
      661 ccagaacacc tccagcagtt tttcataact gatttgcttc gggtcgtagg cgatttgtac
      721 cgcctcgaca tgaccggtgt cgcccgcgga aacctgctcg tagctcgggt tcactttttg
      781 cccgccggtg tagcccgagg tggtggcgct tactcccggc aacgcatcaa acggaggctc
      841 catgcaccag aagcagccgc cggcgaatat cgccgtggcc gtcgctgttt ttcccttcgc
      901 tgccggctcc gccgccgttg ccgccagcgt catgcccaac actgctccac ccagcaccgc
      961 cacgaatcgg gatatgttca tgcgcatcgg gaaccctcat gttttgagtt gaattgcttg
     1021 tgggctttat gacccatctt tgggtcaatg gttgccaggc tgataaccgg ccggaaacaa
     1081 aaaagccacc tgacgggcgg cctttggctt cagttgggtt tgaaaaaaat gaatacgagc
     1141 gtgcggctag gggcgaagcg gagccgtgtt gccgacagtc gcaggcacga cgggtccggt
     1201 ggcctcggcc agggcgggtt gtgcaagcgg tatgtttgcg ttcctggcgc agttgctcaa
     1261 caccagctcg ggcggatcga cggagaaggt gaacggggcg gccttgttga gggcggagag
     1321 gccgagaatc tggcgcgtgc gcgccgggaa cacggctgcc tcgacgttgt ggatggtgca
     1381 gccgccgatg cgcagctgat tgatcgcgta gaccggaata acgagctcgg aaccgttggc
     1441 cagaattgcc cgcaattctt ttacatattg tgcctgtttg cgttcctgca acgcattcag
     1501 cgtttgttcg ttgatggtca catagcccga gccggtatcg accatgaact cgctggcgcc
     1561 gagatcggcg acctgggcgt tgacgtaata ggtagctctt cccttgtcat gcatgggtac
     1621 cgtaacgtcg aactcgcccg ccaggacagg agatgaaacc aggatcagcc cgagggccag
     1681 acaggaataa tgtaacgcgg atagagacat gcttccccac ataagccgtg ccggtcttca
     1741 gtctccggcg tgtcgtgttg cgaaactctc agcaccttca ataggaaata gcggagactt
     1801 tcgcaaagat caagcaaagg ggtacagcga tgccctggac cgttcgtaaa tgccgctttt
     1861 ttgaggttgg gggcgggaaa gaccccattc ccgcagacgg ggaatggggg tacagatccc
     1921 cgcacgggga agatacagat tgttacttgg tttccttcgg catggtctcc agcgcggcga
     1981 attccgccgc atcgagcttg ccgtccttgt tgacatcggc ctgggtgaac ttggccttga
     2041 cctcgggcga tttcttggcc tcgtcgagcg tgatctggcc gtcacggttc gcgtccaggt
     2101 cctggaacga agccgcgccg ctgccggccc aagccaagcc cgagactgtc attagtgcca
     2161 acgcggtaag tgtgtgtttc ttgatcatgg tgacctcctt tgtctgtgga taatctaaac
     2221 cgcttcctga atcaaggaag cggtctatta gtaacaaact ctcgaccatg gtttccactc
     2281 gccccgattg gtggttcatc aaccctgatc ggcggttgat gcagtgatga aaactcaata
     2341 acaccttaca ttcattg
//
LOCUS       DBNGZE010000249         5124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_590285, whole genome shotgun sequence.
ACCESSION   DBNGZE010000249 DBNGZE010000000
VERSION     DBNGZE010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_590285"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1214
                     /gene="rodA"
                     /locus_tag="AC16FV_06495"
     CDS             138..1214
                     /gene="rodA"
                     /locus_tag="AC16FV_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR2849741.1"
                     /translation="MDLQRLHLDKTLFYALLALAAISLFVVYSGSAKDVSATMGHLMR
                     LLLGFTILLVVAQIRPEILERWSPYVFVAGMLLLIAVLAVGVIGKGAQRWLHLGFLRF
                     QPAEVMKLAVPMMLSWFLARDNLPPTLFRLALAFLMMAIPAFMIIKQPDLGTALLVFG
                     AGVFVLFLGGMRWRVIVTLLAIIAVAAPFAWNHLHDYQRQRVLTLFNPEADPLGTGYH
                     TIQSMIAVGSGGIYGKGWLNSSQAHLDYLPESSTDFIFAVYAEEFGLLGSLLLLAIYV
                     IIVARGLLIAYLAKDTYTRLLAGSLSLTFFLYFFVNMGMVTGILPIVGVPLPVMSYGG
                     TSIVTIMAAFGILMSIQTHRKIAE"
     gene            1229..2239
                     /gene="mltB"
                     /locus_tag="AC16FV_06500"
     CDS             1229..2239
                     /gene="mltB"
                     /locus_tag="AC16FV_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR2849742.1"
                     /translation="MHSACPGRLPIICAALAMLAVTSGAQALEFKKNPALRSFTAEMT
                     EKHGFSAKQLRRVFRCATIRPDIIEAMERPRELPPWYEYRKTFVTEDNAIRGVRFWKE
                     HADDISRARARYGVSPEIIVAIIGVETRYGRNPGNYPILDALTTLTFEYPSRSAFFRG
                     ELEEFLLLARENSLDPCRVKGSYAGAMGFPQFMPSNYRRLAVDFDGDGKRDLLDSPAD
                     AIGSVAHYLHNNGWDMNAPVTDEAQLEGTLYFWVEKLGLKPALSVRQLAGYGIFPRHR
                     DNPERRAALISLEGENGPIYRLGYNNFYVITRYNHNKRYAMAVAELGEMIRQRMEENP
                     SS"
     gene            2236..3111
                     /locus_tag="AC16FV_06505"
     CDS             2236..3111
                     /locus_tag="AC16FV_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR2849743.1"
                     /translation="MTSRWLLLIPLLMLLSACGTLTRPGGYYEDDGPEVSPPADVANI
                     PDAVPKTEPRSASGNKPYSVYGVTYTPLSDTSGYRERGIASWYGKKFHGKRTSSGEAY
                     DMYAMTAAHKTLPLPSYLRVRNLQNDRSVVVRVNDRGPFLHNRLIDLSYAAAAKLGIL
                     GTGTGVVEIEAVSADEPSTQVVKTYPLRMIPPAAAAEEIPDSPAAATGPKLYLQVGAF
                     AQRDNAISLRNRLEREALRPIFVQSSQAGGNGGTDPAPIYRVRIGPLASVEEGDRLTE
                     RVAQLGIPDARIVVE"
     gene            3401..4543
                     /locus_tag="AC16FV_06510"
     CDS             3401..4543
                     /locus_tag="AC16FV_06510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR2849744.1"
                     /translation="MLKVIKKFPILLFACLIFSHAVAAPPEIPPPDIDAPAWILVDHN
                     SGQVLAGRNSDKPLPPASLTKLMTAYVLFAKLKAGKLRLDDEVAISAQAVKTNGARLF
                     LRPGTTARAEELLQGMIVVSANDAAIALAEHVAKNEANFVMEMNVTARALELRQTLFV
                     NATGHDEKGHVSTAHDLARLTSALIRDFPEYYKWFARKEFSYQDITQHNHNALLWREP
                     GVDGVKTGHTRAAGYCLSASATHEGMRLIATVLGARDDSARVSAGQKLLAHGFRHFET
                     RLLYAADVPALRVRLWMGDRSTLPLGVRQNLYLTLPRGWHEKLHARLVVKESLAAPVR
                     QGQVMGALALELDRQPYAEYPLLALQEIGTGNILQRAIDRVQLWLQ"
     gene            4625..>5124
                     /locus_tag="AC16FV_06515"
     CDS             4625..>5124
                     /locus_tag="AC16FV_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR2849745.1"
                     /translation="MQHVYLNGEFLPSDQARVSVFDRGFIFGDGVYEVIPVFGGRLFR
                     LPHHLSRLDASLSAIRLRNPHTPQEWNNIFTRLLAEGTGDQSIYLQITRGVAPRDHAF
                     PPNITPTVFAYAQPLKYSPPEQLAQGVAAVTAADIRWSRCDIKAIALLANALLRQEAI
                     DQGAVEA"
BASE COUNT          962 a         1670 c         1504 g          988 t
ORIGIN      
        1 tcgcgcgcaa ggtcatggac cacgtgatcc tcggcaaaac cgccgccgcg cccggcgcgg
       61 cggcaccggc gtcatcgccg gaaaacgacg aatgaaaccg ggacgaatga acgcatctgg
      121 ggttaaatag cgctcacatg gacttgcaac ggctgcatct cgataaaact cttttctacg
      181 cgctgctggc gctggcggcc atcagcctgt tcgtggtcta cagcggcagc gccaaggacg
      241 tgagcgccac catgggtcat ctcatgcggc tgctgctcgg attcacgatc ctgctggtcg
      301 tggcgcagat acggcccgag attctggagc gctggtcacc ctatgttttc gtcgccggca
      361 tgctgctgct gatcgccgtg ctcgccgtcg gcgtgatcgg caagggtgcg cagcgctggt
      421 tgcatctcgg ctttctccgg ttccagccgg cggaggtcat gaagctggcg gtgccgatga
      481 tgctttcgtg gtttctcgcg cgtgacaacc tgccgcccac gctgttccgg ctggcgctgg
      541 cctttctcat gatggcgatc ccggcgttca tgatcatcaa gcagcccgac ctcggcacgg
      601 cgctgctcgt gtttggcgcg ggggttttcg tgctgtttct cgggggcatg cgctggcgcg
      661 tgatcgtcac cctgctcgcc atcattgccg tcgccgcgcc gtttgcgtgg aatcacctgc
      721 acgattatca gcgccagcgc gtgctcacgc tgttcaaccc ggaggccgat ccgctcggca
      781 ccggctatca caccatacaa tccatgatcg ccgtcggttc cggcggcata tacggcaagg
      841 gctggctcaa ttccagccag gcgcatctcg attatctgcc cgagtcctcc accgacttca
      901 tctttgccgt gtacgccgag gaattcggtc tgctcgggag cctgctgctg ctcgcgattt
      961 acgtcattat cgtcgcgcgc ggcctgttga tcgcttatct ggccaaggac acatacacgc
     1021 gcctgctggc gggtagcctg agcctgacgt tcttcctgta tttcttcgtg aacatgggca
     1081 tggtcaccgg catcctgccc atcgtcggcg tgccgctgcc ggtgatgagt tatggtggga
     1141 cttccatcgt cacgatcatg gccgcgttcg gcatcctcat gagcatccag acgcatcgca
     1201 agatagccga atagtattta aatttaatat gcattccgcc tgcccgggaa gattgccgat
     1261 tatttgcgcc gccctggcca tgttggcagt cacttccggc gcacaggcgc ttgaattcaa
     1321 aaaaaacccc gcgctgcgaa gtttcaccgc cgagatgacc gagaagcatg gtttttccgc
     1381 caaacagctg cggcgggtgt ttcgctgcgc caccattcgt cccgacatca tcgaggccat
     1441 ggagcgcccc cgggagctgc cgccatggta tgagtaccgg aaaaccttcg tgaccgagga
     1501 caacgcgata cgcggcgtcc gcttctggaa ggaacacgcc gacgatatct cccgtgcgcg
     1561 cgcgcgttac ggcgtatcac ctgaaatcat cgtcgccatc atcggcgtcg aaacccgcta
     1621 tgggcgcaat cccggcaact atcccatcct cgatgcgttg accacgctga cgttcgagta
     1681 tccgtcgcgt tcggcctttt tccgcgggga gcttgaggaa ttcctgctgc tggcgcgcga
     1741 gaacagcctc gatccctgtc gcgtcaaggg ctcctacgcc ggggccatgg gttttccgca
     1801 attcatgccc agcaactacc gccggctggc ggtggacttc gacggcgatg gcaaacgcga
     1861 cttgttggac agtcccgcgg acgccatcgg cagcgtcgcg cactatctcc acaacaacgg
     1921 ctgggatatg aatgcgcccg tgaccgacga ggcgcaattg gaaggcacgc tatacttctg
     1981 ggtcgaaaaa ctgggcctga aaccggcgct gtccgtgcgc caattggcgg gttacggcat
     2041 cttcccgcgc catcgggata atccggaacg gcgtgccgcg ctcatttcgc tcgaggggga
     2101 aaatggcccc atctaccggc tgggctacaa taatttctac gtcatcaccc ggtataatca
     2161 caacaaacgc tacgccatgg ccgttgccga gctgggcgag atgatacgcc aacgcatgga
     2221 ggagaatccg tcatcatgac ctcacgctgg ttgctgttga ttcccctgtt gatgctgctt
     2281 tccgcctgcg gcaccctcac gcgtcccggc ggctattacg aagatgacgg ccccgaagtc
     2341 agcccgccgg ccgacgtcgc gaatatcccg gatgcggtac cgaaaaccga acctcgctcc
     2401 gcttccggca acaaacccta ctcggtctac ggcgtcacct acacgccgct ttcagacacg
     2461 agcggttacc gcgagcgcgg catcgcctcc tggtatggga aaaaatttca tggcaagcgc
     2521 acttcgagcg gcgaagccta cgacatgtat gccatgacgg cggcgcacaa gacgctcccg
     2581 ctgccttcgt acttgcgcgt gcgcaatctg cagaacgacc gttccgttgt cgtgcgcgtc
     2641 aacgaccgcg gaccttttct gcacaaccgc ctgatcgatc tttcctacgc cgcggccgcc
     2701 aaactcggca ttctcggtac cggcaccggc gtcgtggaaa tcgaagccgt cagcgccgac
     2761 gaaccctcga cacaagtggt caaaacctat ccgctccgga tgatcccgcc cgccgcggcg
     2821 gccgaggaaa tacccgattc gccggcggcg gcgaccggtc ccaagcttta tcttcaggtg
     2881 ggcgcgttcg cgcaacggga caacgccatc agcctgcgca accgtctcga acgcgaggcg
     2941 ttgcgaccaa tattcgtcca gtcttctcaa gccggcggga acggtggcac ggatccagcc
     3001 ccaatctacc gcgtgcgcat cggtccgctg gccagcgtgg aagaaggcga ccgcctgacg
     3061 gaacgcgtcg cgcagcttgg cattcctgat gcccgcatcg tcgtcgaata atcgtttgca
     3121 aaaaatcaat gaacaggatt aacagggcga gccggaaaca gacgaagaca cttaacgtgt
     3181 cttcgtcccg gcgagcgccc cgccacggat ggcggggccg agtatgtcga agttttcatg
     3241 gcctcgccag ggacggcata cattccgtcc caggcgttgc caccgtagct tcgatacacc
     3301 ccggcccgca cttccctgtg cgggcgctca gcggcgccga tgtacgttaa gtacatcggc
     3361 gacatctccg cttcgccctg ttaatcctgt gaaatttctc ttgttaaaag ttattaaaaa
     3421 attccccatt ctgcttttcg cgtgcctgat attcagccac gcggtcgcgg cgcctcccga
     3481 gattcccccg ccggacatcg atgcgccagc gtggatactc gtggatcaca acagcggtca
     3541 ggtgctggcg gggcggaaca gcgacaagcc gctcccaccc gcgagcctca ccaagctcat
     3601 gacggcctat gtgctgttcg cgaaactgaa ggcggggaaa ctgcggctcg acgacgaggt
     3661 cgccatcagc gcgcaggccg tcaaaaccaa cggggcgcga ctgttcctgc gccccggaac
     3721 cacggcgcgc gcggaggaac tgcttcaggg catgatcgtg gtttccgcca acgacgcggc
     3781 cattgccctc gcggaacacg tggcaaagaa cgaggcgaac ttcgtcatgg agatgaacgt
     3841 gacggcgcgt gcgctcgaat tgcgccagac cctgttcgtg aatgccacgg gccatgacga
     3901 gaaggggcat gtatcaacgg cgcacgatct cgcgcgcctg acctcggccc tgatccgcga
     3961 ctttcccgaa tactacaagt ggttcgcgcg caaggaattc agctaccagg acataaccca
     4021 gcacaatcac aacgcgctgc tgtggcgcga accgggggtc gatggagtga aaaccgggca
     4081 cacccgcgcc gccggctatt gcctcagcgc ctcggccacg cacgagggca tgcgccttat
     4141 cgccacggta ctcggcgccc gcgacgacag cgcacgagtg agcgccggac agaaactgct
     4201 ggcacacggg tttcgccatt tcgagacgcg cctgctctac gccgccgatg tgcccgctct
     4261 gcgtgtgcgc ctgtggatgg gcgaccggtc cacgcttccg ctcggcgtgc gccagaatct
     4321 gtatctgacc ctgccccgcg gctggcatga aaagctgcac gcgcggctgg tggtgaagga
     4381 gtcgctggcc gcgcccgtgc ggcaggggca ggtgatgggc gccctggcgc tggagctcga
     4441 ccggcagccc tacgcggaat accccttgtt ggcgctccag gagatcggca cgggcaatat
     4501 acttcaacgc gctatcgacc gagtccagct ctggctgcaa taacaacgta aaccaaacgt
     4561 gggtcgttgg aaggccgcgt taacgtttat acttcgcgat tcactactga cgattcaacc
     4621 atccatgcaa catgtctatc tcaacgggga atttctcccg tccgatcagg cccgggtatc
     4681 ggtcttcgat cgcgggttta ttttcggcga cggcgtctac gaggtcattc cggtattcgg
     4741 cggtcggctg ttccgcctgc cgcatcatct ctcacgcctg gacgcgagcc tctccgccat
     4801 ccgtctgcgc aatccgcaca caccgcagga atggaacaac attttcacgc gcctgctggc
     4861 ggagggaacc ggcgatcagt ccatctattt gcagatcacg cgcggcgtcg cgccgcgcga
     4921 ccacgcgttc ccaccaaaca tcacgccgac ggtgttcgcc tacgcccagc cactgaaata
     4981 ctccccgcct gagcaactgg cgcagggcgt ggcagccgtg accgcggccg acatccgctg
     5041 gagccgctgc gacatcaagg cgatcgcgct gctggccaac gcgctgctgc ggcaggaggc
     5101 gatcgaccag ggcgccgtgg aggc
//
LOCUS       DBNGZE010000250         3798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_936872, whole genome shotgun sequence.
ACCESSION   DBNGZE010000250 DBNGZE010000000
VERSION     DBNGZE010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_936872"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="AC16FV_06520"
     CDS             <1..675
                     /locus_tag="AC16FV_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2849746.1"
                     /translation="TSSAQVAQDFVAKYGSSNTVLKTTLGWSHDTLDSLLLPTAGSLQ
                     RLSAEIAVPGTDIEYYKLTYMAGRYWPLTEAFTFKMKGELGYGNSYGNTDQLPFYKNF
                     YAGGSSTVRGYRSRSLGPRDTLPPNDPIGGNRRVLVNAELLFPLPGSSTDNKSMRLSL
                     FTDGGMVYGPGEKVELGQLRYSAGLAFNWFSPVGPLSFSYAIPLNDKPGDRTESFQFT
                     LGVPLR"
     gene            691..1203
                     /locus_tag="AC16FV_06525"
     CDS             691..1203
                     /locus_tag="AC16FV_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR2849747.1"
                     /translation="MRIRTIVFRTAICLLCACALPAVAVSAELKIGYVNAVKVIEEAP
                     QGEAALKKLETEFAPRDKQIVEMQNKLKQLEQDLEKNALVLKDTEHRSKEFEIVSLKR
                     DLRRATQEFREDYNLRRNEELAALQKIVQKTIAEIAKQENYDLVLESAVYAGPRADIT
                     EKILKKLGKK"
     gene            1210..2247
                     /gene="lpxD"
                     /locus_tag="AC16FV_06530"
     CDS             1210..2247
                     /gene="lpxD"
                     /locus_tag="AC16FV_06530"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR2849748.1"
                     /translation="MAVTLAELARRFQGRVRGNPDLVIERVAPLENAGPHDIAYLSDR
                     KYLPKLAHTAAGAVILTETDSSRYAGSALIVTNPHLCFARVAQWLHPSPAFQPGVHPT
                     AVIIPSSRIARTAWIGPHSVVEGGVEIGDDAYVGPGCFIGNHVTIGARSRLVGHVSIG
                     DRCIVGEDCLLHPGVVIGSDGFGYAKDGDQWQKVPQLGRAVIGKNVEVGANTTIDRGA
                     LNDTVIGNGVKLDNQIQIAHNVHIGDNTAIAACVGIAGSTRIGMRCTIGGQAGIVGHL
                     EIADDVHITAGSLVTSSIRQAGAYSSSLKAEPAEKWRRNAARLHHLDEMAQRLGKLEE
                     KLQQHSEESEN"
     gene            2244..2687
                     /gene="fabZ"
                     /locus_tag="AC16FV_06535"
     CDS             2244..2687
                     /gene="fabZ"
                     /locus_tag="AC16FV_06535"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011759266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR2849749.1"
                     /translation="MNSLDIHEILKYLPHRYPFLLVDRVLTWTKDESITAIKNVTINE
                     PFFQGHFPNRPVMPGVLIIEAMAQSCAILSFKSMDALPKANSIFYFVGIDNARFKRPV
                     EPGDQLRFTSKIKRKVKGIWMFESSAHVGDELCCSAELMCTYKEA"
     gene            2688..3455
                     /gene="lpxA"
                     /locus_tag="AC16FV_06540"
     CDS             2688..3455
                     /gene="lpxA"
                     /locus_tag="AC16FV_06540"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR2849750.1"
                     /translation="MIHPLAIIDPGAKIGKDVQIGAFTIIGAGVEIGNGTWIGPHVVI
                     NGPTRIGDENRIYQFSSLGEGPQHLGYKGEPTWLEIGDRNIIREYCTFNRGTVAGGGV
                     TRLGHDNFIMAYCHLAHDCQVGNRTVFANGTSLAGHVRVEDQVIFGGFTMIHQFCRVG
                     AHSMTGINTVTFKDIPPYLLVAGNTAVPHGLNVRGLKRRNFSEQTIESLRQAYKLIYK
                     SGLRLSEATEQLGQAATNPEVRHFLEFIKQSERGIVR"
     gene            3474..>3798
                     /locus_tag="AC16FV_06545"
     CDS             3474..>3798
                     /locus_tag="AC16FV_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR2849751.1"
                     /translation="MTRIGIVAGEASGDLLGAGLIREIRKRLPTASFEGIGGPQMQAV
                     GCCSLFPMDRLSTIGFEGVEKYPEILGIMNKLTEHFLSNPPDLFIGVDVPDFNLGLEE
                     KLKAAG"
BASE COUNT          832 a         1052 c         1140 g          774 t
ORIGIN      
        1 accagcagcg cgcaggttgc ccaggatttc gtcgccaaat acgggtcatc gaatacggtt
       61 ctgaagacaa cgttgggatg gtcgcacgac acgctggata gcctgctgct gcccaccgca
      121 ggcagcctgc agcggctaag cgccgaaatt gccgtgccgg gcaccgatat cgaatactac
      181 aagctgactt acatggccgg ccgctactgg ccgctcaccg aggcctttac cttcaagatg
      241 aagggtgagc tcggttatgg caatagttac ggcaatacgg accagctgcc gttttacaag
      301 aatttctacg ccggtggcag cagcacggtg cggggctacc gctcgcgttc tctgggcccg
      361 agagacaccc tcccgccgaa tgatcccatc ggcggcaacc gacgggtgct ggtgaatgcg
      421 gaattgctgt ttccgctgcc gggttcgtcc actgacaaca agtccatgcg gctcagtctt
      481 ttcaccgacg gcggcatggt gtacggcccg ggtgaaaagg tcgaactcgg ccagttgcgc
      541 tattcagccg gtttggcgtt taattggttc tcgcccgtcg ggccgctcag cttcagttat
      601 gcgattccgc tgaacgacaa accgggcgat cggaccgaat cgttccagtt cacgctgggt
      661 gtgccgctgc ggtaaataca ggaggttgtt ttgcgtatca gaaccatcgt attcaggacc
      721 gccatatgtt tgctgtgcgc ctgcgcgctc ccggcggtgg ccgtatcggc cgaactcaag
      781 atcggatacg tcaacgccgt caaggtcatc gaggaggcac cgcagggtga ggccgcgctc
      841 aagaaactcg agaccgagtt cgcgccgcgt gacaagcaga ttgtcgagat gcagaacaag
      901 ctcaagcagc tcgagcagga cctcgaaaag aatgcgctgg ttctgaagga taccgagcac
      961 cgttccaagg aattcgaaat cgtttccttg aagcgcgatt tgcgccgcgc cacgcaggaa
     1021 ttccgcgagg attacaatct gcggcgcaac gaggaactgg ccgcgcttca gaagatcgtg
     1081 caaaagacga tcgcggagat cgccaagcag gaaaactacg atctcgttct ggaaagtgcc
     1141 gtttacgccg gtcccagggc ggacatcacc gaaaaaatcc tgaaaaagct gggaaagaaa
     1201 tagcgcacga tggccgtcac tctcgcagag ctggcgcgac gtttccaggg cagggtgcgg
     1261 ggaaatcccg atctcgtgat cgagcgcgtg gcgccgctgg agaacgccgg cccgcatgac
     1321 atcgcctacc tgagcgacag gaaatatctc ccgaaactcg cgcataccgc ggctggcgcg
     1381 gtgattctga ccgagaccga ttcatcccgc tatgccgggt ctgcgctgat cgtcaccaac
     1441 ccgcatttgt gtttcgcgcg cgtggcgcaa tggctgcatc cgtctccggc gtttcagcct
     1501 ggcgtgcatc caacggcggt aatcattccc tcgtcgcgta tcgccaggac ggcgtggatt
     1561 ggaccgcatt ccgtggttga gggcggtgtc gagatcggag atgatgcgta cgtcgggccg
     1621 ggttgtttca taggaaatca tgtgacgatc ggcgcgcgct cccggcttgt cgggcatgta
     1681 tccatcggtg atcgttgcat tgtcggcgag gattgcctgc ttcatcccgg ggtggttatc
     1741 ggcagcgatg gttttggtta cgccaaggac ggggatcagt ggcagaaggt gccgcaactc
     1801 gggcgcgctg ttatcgggaa gaatgtggag gttggcgcca acaccaccat cgatcggggc
     1861 gccctgaatg ataccgtcat cggcaatggc gtgaagctgg ataaccagat acagattgcc
     1921 cacaatgtgc acatcggcga taacacggca atcgcggcct gcgtgggcat tgcgggcagc
     1981 acaaggatcg gcatgcgctg cacgatcggc gggcaggcgg gtatcgtcgg acatctggaa
     2041 atcgccgatg acgtgcacat taccgcggga tcgctggtga cgagctcgat caggcaggcc
     2101 ggcgcctatt catcaagcct caaagccgaa ccggcggaga agtggcgccg caatgcggcc
     2161 agactgcatc atctcgacga gatggcgcaa cggcttggca agctggaaga aaaactgcaa
     2221 cagcattccg aggagtctga aaattgaact cgctggacat ccatgaaatc cttaaatatc
     2281 ttcctcatcg ctatccgttt ctgctggtcg accgcgtgct gacatggacg aaggatgaat
     2341 cgatcacggc catcaagaat gtgacgatca acgaaccctt ttttcagggc cactttccca
     2401 accgtcccgt gatgccgggc gtattgatca tcgaagccat ggcgcagtca tgcgccattc
     2461 tttcgttcaa gagcatggac gccttgccaa aggcgaattc cattttttat tttgtcggca
     2521 tcgacaacgc gcgcttcaaa cgtccggtgg agcccggaga tcagctcagg tttacgtcga
     2581 aaatcaagcg gaaagtaaag ggaatctgga tgttcgagag cagcgcccat gtgggcgacg
     2641 aattgtgctg cagcgccgaa ttgatgtgca cctataagga agcctgagtg atccatcccc
     2701 tggccattat tgatccgggc gccaagatag gaaaagacgt tcaaatcggc gcatttacca
     2761 tcattggagc cggcgtcgag atcggcaacg ggacctggat cggaccccat gtcgtgatca
     2821 acggtccgac acgtatcggc gacgaaaacc gtatttacca gttctcgtca ctgggagagg
     2881 ggccgcaaca tctgggctac aagggtgaac cgacctggct cgagattggc gaccggaaca
     2941 tcatccggga atactgcacc ttcaatcgcg gcacggtggc gggtgggggg gtcacgcgac
     3001 tgggccacga caacttcatc atggcgtact gtcatctggc gcacgattgc caggtcggaa
     3061 accgcacggt tttcgcgaac ggcacgagtc tcgccggtca cgtgagagtg gaagatcagg
     3121 taattttcgg cgggtttacc atgatccatc agttctgtcg cgtcggcgcg cactcgatga
     3181 ccggcatcaa taccgtgacc ttcaaggata taccgcctta tctgctggtg gcgggcaata
     3241 ccgccgtacc tcacgggctg aatgtacgcg ggctcaagcg ccggaatttt tccgaacaga
     3301 cgattgaatc tcttcggcaa gcctacaagc tgatatacaa atccggtctg cgactcagcg
     3361 aggcgaccga acagctggga caggcggcca cgaaccccga agtcagacac tttctcgaat
     3421 tcatcaagca gtccgaacgc ggaattgttc gttgatccgt ggcggcaagg cccgtgacaa
     3481 gaatcggaat cgtcgccggc gaggcttccg gcgatttgct gggcgccgga ctgatacgcg
     3541 aaatcaggaa acggcttccg acggcttcct tcgagggtat cggcggtccg caaatgcagg
     3601 ccgtgggttg ctgcagcctt tttcccatgg accggctttc taccatcggt ttcgagggtg
     3661 tcgagaagta tccggagatt cttggcataa tgaacaagct tacggaacat tttttgtcca
     3721 acccgccgga tctcttcatc ggtgttgatg tccctgattt caatcttggt ctcgaggaaa
     3781 agctgaaggc cgccggta
//
LOCUS       DBNGZE010000251         2916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1353535, whole genome shotgun sequence.
ACCESSION   DBNGZE010000251 DBNGZE010000000
VERSION     DBNGZE010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1353535"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="AC16FV_06550"
     CDS             complement(<1..155)
                     /locus_tag="AC16FV_06550"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR2849752.1"
                     /translation="MWRYLWIAVVVLVFDQLTKFMVADYLTKHGEIHLAPFLNFVLVH
                     NTGAAFG"
     gene            complement(155..2908)
                     /gene="ileS"
                     /locus_tag="AC16FV_06555"
     CDS             complement(155..2908)
                     /gene="ileS"
                     /locus_tag="AC16FV_06555"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005369962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR2849753.1"
                     /translation="MAKREPQWVRQWHDKGVYRRLRAIAKGRPRFVLHDGPPYANGDI
                     HIGTAVNKILKDIIVKARTLSGFDAPYVPGWDCHGLPIEHAVEKKIGKAGTQVEPAKF
                     RQACREYARVQVDRQREDFIRLGVVGDWQRPYLTMDFSIEADIIRALGRIIANGHVVR
                     GHKPVHWCSDCGSALAEAEVEYMDKTSPAIDVRFTVVDPAAFLARCEHAAGHTGEGPI
                     HVVIWTTTPWTLPANQAVALHPDLEYVLLQCSSPTGHERLLIADPLVKDVMARYGIDQ
                     YRVVANCRGKDLEGVKLKHPFYAREVPIILGEHVTTDAGTGAVHTAPGHGLEDYVVGE
                     RYELAVDNPVGEDGKFLPGTELFAGEHVFKANDHVVEVLKTRGALLHAEALNHSYPHC
                     WRHKTPIIFRATSQWFISMERLREPALRAIMGVRWVPDWGQARIQGMIENRPDWCISR
                     QRAWGVPIPLFVHRQTGEAHPNTDRLIEDVAQRVEKQGIDAWFDLKPRDLLGADAENY
                     EKVSDTLDVWFDSGVTHSCVLERRPELPFPADLYLEGSDQHRGWFQSSLLTSVAMRGE
                     APYKSVLTHGFTVDAKGRKMSKSLGNVIVPQKVIKVMGADILRLWVAATEYRAEMSIS
                     DEILTRMADSYRRIRNTTRYFLANLSGFDTASPLPVKDMLALDQWALARAHDLQKEVL
                     EAYEQFQFHLIYQKLHQFCVVDMGGFYLDILKDRMYTMPTNSRGRRSAQTAMFHILEA
                     LVRWLAPVLSFTAEETWRYMPGKRGESVFLEKWHSLPALPDGAVDRAFWGEVIGVRQA
                     VAKELEKLRVAGSIGSGLGAEVDIYCDDSWQGRLRRLGDELRFVFITSYLRVHPLSDK
                     PSDAVETEIKGLAVRAAPSAFTKCIRCWHHREDVGANKEHPQICGRCVQNVTGPGETR
                     SYA"
BASE COUNT          610 a          923 c          830 g          553 t
ORIGIN      
        1 aaaccgaagg ccgcaccggt gttgtggact aatacaaaat taaggaaagg tgcaagatga
       61 atctcgccat gcttggtcag ataatcggcg accatgaatt tcgtcagctg atcgaacacc
      121 agcacgacaa cggcaatcca caagtaccgc cacatcaggc gtaactccgt gtctcgcccg
      181 gcccggtcac attctgcacg cagcggccgc agatctgcgg gtgttccttg tttgcaccga
      241 catcctcgcg gtgatgccag cagcgaatac acttggtgaa ggccgagggc gcggccctta
      301 ccgccaatcc ctttatttcc gtttcaaccg catcggaagg cttatctgac aaaggatgta
      361 cccgcagata ggaagtaatg aaaacaaatc gcaattcatc tcccagacga cgtaaccggc
      421 cctgccagga atcatcacaa tagatatcca cttccgcgcc gagaccagaa ccaatgctgc
      481 cggcgacacg cagcttttca agctccttgg ccacggcctg acgcacacca atcacctcac
      541 cccagaaagc gcggtcaaca gcaccatcgg gcaacgcggg cagtgaatgc catttctcca
      601 gaaagacaga ctcgccacgc ttgccgggca tatagcgcca ggtttcctca gcagtgaaag
      661 acaacaccgg cgccagccag cgcaccagtg cttccagaat atgaaacatg gcagtttgag
      721 ccgagcgacg cccgcggcta ttggtcggca tggtgtacat ccgatccttg aggatgtcga
      781 gatagaaacc gcccatgtcc accacgcaga actgatgcag tttctgataa atcagatgga
      841 actggaactg ctcgtaagcc tccagcactt ctttctgcaa atcgtgcgcc cgcgccagcg
      901 cccactgatc gagcgcaagc atgtctttca ccggcagtgg tgacgcggta tcaaagccgg
      961 agagattggc cagaaaataa cgcgtggtat tgcggatgcg ccggtaggaa tcggccatgc
     1021 gcgtgaggat ctcgtcggag atactcatct ccgcgcggta ctcggtggcc gccacccaca
     1081 gacgcaggat atccgcaccc atgaccttga tcaccttctg cggcacgatt acgttgccaa
     1141 gcgacttcga catcttccgg cccttggcgt ccacggtgaa tccatgcgtc aacacgcttt
     1201 tataaggcgc ctcgccacgc atcgccaccg aagtgagcaa cgatgactgg aaccagccgc
     1261 ggtgctggtc cgatccttca agatataaat cggcgggaaa cggcagttcg ggtcggcgct
     1321 ccagcacgca actgtgcgtc acgccggaat cgaaccagac atcgagggta tcgctgacct
     1381 tctcgtagtt ttcagcgtcc gcgccaagca ggtcccgcgg tttcagatcg aaccaggcat
     1441 cgataccctg cttttccaca cgttgggcga cgtcttcgat caggcgatcg gtattcgggt
     1501 gcgcctcgcc cgtctgccgg tgcacaaaca gcgggatggg cacaccccag gcacgctggc
     1561 gtgaaatgca ccagtccggg cggttttcga tcatgccctg aatacgcgcc tggccccaat
     1621 ccgggaccca cctgacaccc ataatggcgc ggagggccgg ttcgcgcagc cgctccatgc
     1681 tgatgaacca ctgggatgtg gcgcggaaaa taatcggcgt cttgtggcgc cagcaatgcg
     1741 gataactgtg attcagcgcc tcggcgtgca gcaaggcgcc gcgcgtcttg agcacctcga
     1801 cgacatgatc gttggccttg aatacgtgtt cgccggcgaa aagttccgtg ccgggcagga
     1861 atttgccgtc ctcgcccacc gggttgtcca cggcaagctc atagcgctcg ccaaccacgt
     1921 agtcttccag gccgtgtccc ggcgccgtgt gcaccgcgcc ggtgccggcg tccgtcgtca
     1981 cgtgttcgcc aagaatgatc ggcacctcgc gcgcgtaaaa cggatgcttg agcttaacgc
     2041 cttccagatc cttgccgcgg caattggcca cgacgcgata ctgatcgatt ccgtagcgcg
     2101 ccatcacgtc cttgaccaga ggatcggcga tcaacaaacg ctcatgcccc gtggggctgg
     2161 aacactgcaa cagcacatac tccagatccg gatgcagcgc gaccgcctgg ttggcgggca
     2221 gcgtccacgg tgtcgtggtc cagatcacaa catgaatcgg accttcgccc gtgtgtcctg
     2281 cggcatgttc gcaacgcgcg agaaacgcag ccggatcgac cacggtaaaa cgcacatcaa
     2341 tggccggaga ggtcttgtcc atgtattcga cctcggcctc ggccagcgcc gaaccgcagt
     2401 cgctgcacca gtgcaccggc ttgtggccgc gcaccacgtg gccgttggcg atgatgcgtc
     2461 caagcgcacg gataatgtcg gcttcgatgg aaaaatccat tgtgagataa ggccgctgcc
     2521 agtcgcccac cacgccgagg cggatgaaat cctcgcgttg ccggtccacc tgcacgcgcg
     2581 catattcacg gcaggcttgg cggaatttcg ccggctcgac ctgggttcct gccttgccga
     2641 tttttttctc gacggcgtgc tcgatgggca ggccatggca atcccagccc ggcacgtagg
     2701 gtgcgtcgaa tccgctcagg gtccgggcct tgacgatgat gtccttgagg atcttgttga
     2761 ccgcggtgcc gatatggatg tcgccgttcg cgtacggcgg gccgtcgtgc aggacgaagc
     2821 gcggccggcc cttggcgatc gcgcgcagcc ggcggtagac gcccttgtcg tgccattgcc
     2881 tgacccattg cggctcgcgc ttcgccagat cgccgc
//
LOCUS       DBNGZE010000252         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1007543, whole genome shotgun sequence.
ACCESSION   DBNGZE010000252 DBNGZE010000000
VERSION     DBNGZE010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1007543"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..534)
                     /locus_tag="AC16FV_06560"
     CDS             complement(184..534)
                     /locus_tag="AC16FV_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849754.1"
                     /translation="MNTRKFFFSAAHFALLSACALALAACATDGTGPTAADAPKAEIA
                     TAPKAETPRIQAAKPSEPVRDTEPMTRSRAARECWMRTEKGNTREDLDKRADHVNKCI
                     EEKMRAASTPAAKT"
     gene            complement(1249..>2664)
                     /locus_tag="AC16FV_06565"
     CDS             complement(1249..>2664)
                     /locus_tag="AC16FV_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR2849755.1"
                     /translation="GIKHELHQRLDQLNAAGRLLEAQRLEQRTIFDLEMMEATGSCAG
                     IENYSRYLTGRKPGEPPPTLFEYVPDNALVFADESHVTIPQLGGMFRGDFRRKATLAE
                     YGFRLPSCMDNRPLRFEEWDAMRPQTMAVSATPGPWEMEEARGVFVEQVIRPTGLIDP
                     PVDVRPARTQVDDLVGEVRQVAQKGFRSLITVLTKRMAEDLTEYLHEQGIRVRYMHSD
                     IDTLERIEIIRDLRLGAFDALVGINLLREGLDIPECALVAILDADKEGFLRSETSLIQ
                     TIGRAARNVDGKVVLYADAVTGSMQRAIEETNRRREKQEAYNIANGITPESIKRSIHD
                     ILDSVYERDHVLISTGAGEGEFAEAATIGHNLEAVIVDLETRMREAAADLDFELAARL
                     RDEIKRLRATELAVLDNPTARDVMVAQTKGLQPRSRPHQPELDEMGIALSHEVKPARA
                     GSPRSKLGRPGMRGGWKGKQR"
BASE COUNT          431 a          806 c          835 g          592 t
ORIGIN      
        1 gcgccagctt ataggcggag agttgcttgg gtttgtcctt gtcggctttc ggctttttct
       61 tttccttggc ggctcgcatc tgaccttttg ccatcacgtc ctcctgaaga ggtctgggga
      121 tgctgaatgc taatcaccgc gccggctggc gcaaggggat ggcggcaagg ctcaggataa
      181 cctctaggtt tttgccgccg gtgtgcttgc cgccctcatt ttctcttcga tgcatttgtt
      241 cacgtggtcg gcgcgcttgt cgagatcctc gcgggtgttg cccttttcgg tcctcatcca
      301 gcattcccgc gcggcgcgcg aacgcgtcat aggctcagtg tcccggacag gctcggacgg
      361 cttggccgcc tggatcctgg gcgtttcagc cttgggcgcg gtggcaattt ccgccttggg
      421 cgcatctgcc gccgtcgggc cggtgccgtc ggttgcgcag gcggcgagtg ccagcgcgca
      481 ggcgctaagc agcgcgaagt gtgcggcgga gaagaagaat ttgcgggtat tcatacagta
      541 cgtttcggac tttcgatttt cggatcgatg gccgccagct ggtcggcgtg gcctggctat
      601 ttgcctgaaa tgacgcgtat acgcaacgcc ggtggaaccc gctttcacaa tcacattcgg
      661 cattatttgc aatacgtcgc agcaggcaga gctgacattc gaagccggca gcggcgggtt
      721 tgctggatac ggcagtgtga gcattggaga cattttgcca aggctggttc tgctagggga
      781 tttggtactc ggcgacagat ttccgaagcc gcacgtcaaa atcacaatgc gtccagaaga
      841 actggcaagc cggtggcagc taggacgatg gttatgctct gtccaaaagc aatcttgatc
      901 tcaccgtcgt cgaaacgaat gggctgccta tgacacatgt atctgtcatc catcgcatcg
      961 gctgcttcgg gcagtgcatc tgttccgtct aacgcggcag actatccgct tggcgcgacc
     1021 ggcaagctac tactgtctct acgcctacga cggatttgtt tatcactatc cttaggacga
     1081 catcgcttgc ctacggctac gcgcgccggc atcattcccg ttatcgcgtt tgatactagt
     1141 aggcgcgtaa caggagttgg cagtgacatt ccgcgccgtt ttgctttctc tgcgctgatc
     1201 gccatcagtg ctggcggttt ggcgatgtca tcgagagccg cgcgtttgtt accgctgctt
     1261 acctttccac ccaccccgca tgcccggtcg gcctaacttg ctgcgcggac tgccggcgcg
     1321 tgccggcttc acctcgtgag agagcgcgat acccatctca tcgagctccg gctggtgcgg
     1381 acgtgagcgt ggctgcaacc ccttagtctg cgccaccatc acatcgcgag cggtcggatt
     1441 gtccagcacg gccagctcgg tggcgcgcag gcgcttgatc tcgtcgcgca ggcgcgcggc
     1501 cagctcgaaa tcgagatccg cggcggcctc gcgcatgcgc gtctcgagat ccacgatcac
     1561 ggcctctaga ttgtggccga tggtcgcggc ctcggcgaac tcgccttctc cggcgccagt
     1621 cgaaatcagc acatggtcgc gttcgtacac gctgtcgaga atgtcgtgga tcgagcgctt
     1681 gatgctctcc ggcgtgatac cgttcgcgat gttgtaggct tcctgcttct cgcgccgccg
     1741 gttggtctcc tcgattgcgc gctgcatcga gccggtgacg gcgtccgcat aaagcaccac
     1801 cttgccgtcg acgttacgcg cagcgcggcc gatggtctgg atcaggctgg tctcgctgcg
     1861 aagaaatccc tctttatccg cgtcgagaat tgcgaccagc gcgcattccg gaatgtcgag
     1921 gccctcgcgc agcagattaa tgccgaccag cgcgtcgaag gcgccgagcc gaaggtcgcg
     1981 gatgatctcg atgcgttcca gcgtgtcgat gtccgagtgc atgtagcgca cgcgaatgcc
     2041 ctgctcgtgc aggtattcgg ttaggtcctc ggccatgcgc ttggtcaaca cggtgatgag
     2101 cgagcggaag cctttttgcg ccacctgccg cacttcgccg acgagatcgt ccacttgcgt
     2161 gcgcgctggc cgcacgtcta ccggcgggtc gatcaggccg gtcgggcgaa tcacctgctc
     2221 gacgaagacg ccgcgcgctt cctccatttc ccaggggccg ggcgtcgcgg acaccgccat
     2281 cgtctgcggc cgcatggcgt cccactcctc gaagcgtagc ggccggttgt ccatgcagga
     2341 gggcaggcgg aatccatatt cggccagcgt cgccttgcgg cggaaatcgc cacggaacat
     2401 gccgccgagc tgcgggatgg tgacgtggct ttcgtcagcg aataccagcg cgttgtcggg
     2461 cacatattcg aacagcgttg gcggcggctc gcccggcttg cgaccggtga gatagcgcga
     2521 atagttctcg atgcctgcac aggagccggt ggcctccatc atttcaaggt cgaagatcgt
     2581 gcgctgctcg aggcgttgcg cttccagcag gcggccggcg gcgttgagct ggtcaaggcg
     2641 ctgatgcagc tcgtgtttga tgcc
//
LOCUS       DBNGZE010000253         3854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1767922, whole genome shotgun sequence.
ACCESSION   DBNGZE010000253 DBNGZE010000000
VERSION     DBNGZE010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1767922"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16FV_06570"
     CDS             <1..375
                     /locus_tag="AC16FV_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR2849756.1"
                     /translation="ITSVAGSSEWFERGFVAYTNLAKREMLGVKTTILSRYGAVSEQT
                     ARAMAEGALTHSHAQVALAITGIAGPSGGTPEKPVGTVCFAWAGKKRDTSSIKQVFSG
                     DRESVRREAVATALQGVLDFIR"
     gene            550..1599
                     /gene="recA"
                     /locus_tag="AC16FV_06575"
     CDS             550..1599
                     /gene="recA"
                     /locus_tag="AC16FV_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR2849757.1"
                     /translation="MDTNKTKALNMALGQIEKQFGKGSVMRLGDAGVVQQVEAVSTGS
                     LGLDIALGIGGLPRGRVVEIYGPESSGKTTLALSVVAQIQKLGGAAAFIDAEHALDPL
                     YAKKLGVKIDDLLVSQPDTGEQALEIADMLVRSGGVDVVVIDSVAALTPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRIGAIKKGEEVIGSQTRVKVVKNKVAPPFRQTEFDIIYNEGISKEGELID
                     IGSESGIVEKTGAWYSFNKERIGQGKDNARQFLKDNPDIAAEIEKRIREKAVVATRSA
                     AAEDEAEEATAEEEA"
     gene            1641..2066
                     /locus_tag="AC16FV_06580"
     CDS             1641..2066
                     /locus_tag="AC16FV_06580"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR2849758.1"
                     /translation="MAMQWLARREHSRRELQDKLLKKGCGAALAAEVVGQLESEHLVS
                     DDRFMESLIRARRSRGYGPLRIQKELQEKGVAPEAISDWLDATSRDWIDDIRRVQRKK
                     FGARLPKNYTERARQARFLQYRGFTYDQIQQLLNPRGQD"
     gene            2079..>3854
                     /gene="alaS"
                     /locus_tag="AC16FV_06585"
     CDS             2079..>3854
                     /gene="alaS"
                     /locus_tag="AC16FV_06585"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR2849759.1"
                     /translation="MKSSEIRQRFLDFFKRHGHTIVPSSPLVPANDATLLFTNAGMVQ
                     FKEVFLGLEKRPYVRAVSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REGIRYAWEFLTQDLKLPPERLWVTVYKDDHEAADIWLKEMKVDPKRFAYLGESSNFW
                     QMGDTGPCGPCSEIFYDHGPGIPGGPPGSPDEDGDRYVEIWNLVFMQYNRDASGKLTP
                     LPKPSVDTGMGLERISAVQQGVHSNYQIDLFQNLIRAAIEITGASDHQSHSLNVVADH
                     IRAATFLIIDGVLPSNEGRGYVLRRIIRRAIRHGHRLGATETFFYKLVGPLCQQMGDA
                     YPELNKERAQVERVLQQEEDRFNETLDQGLKILEDGIAGLKGDTIPGELIFRLYDTYG
                     FPVDLTRDVAMERNLKIDMAGFEREMEAQRERARAASSFKTVEIGAVTVDQPTQFTGY
                     ERLSDEGKILQITRASDLAQTDSLRAGESGNIVLDRTSFYAESGGQVGDRGVLRVGDA
                     IFRVEDTQKHGQIHVHQGRVEKGQFRAGDKVTAEVDAVLRRATMLNHSATHLMHKALR
                     DVLGAHVQQKGSLVDDQHTRFDFSHF"
BASE COUNT          820 a         1124 c         1212 g          698 t
ORIGIN      
        1 atcacctcgg tggcgggcag ctccgagtgg ttcgagcgcg gttttgtcgc ctacaccaat
       61 ctggccaagc gcgagatgct gggcgtgaaa acgaccatcc tttcgcgtta cggtgcggtc
      121 agcgaacaga ccgcgcgcgc catggccgag ggcgcgctga cccattccca cgcgcaagtg
      181 gcgttggcga ttaccggcat cgccggcccg agcggcggca cgccggagaa accggttggc
      241 accgtctgct tcgcctgggc cggcaagaag cgcgacacca gcagtattaa gcaagtcttt
      301 tccggtgatc gcgagtcggt gagaagggag gcggttgcta ccgcgcttca gggcgtgctg
      361 gattttattc gctgagattc cgccgcgctt ttcatcaacc cttatttcgt aaggtcgggt
      421 tttagggccg tggcaggcgc cgtccgcgcg gggctaggcc gtgccggggg agatgtggaa
      481 taatcgtttt cacacacagc ttttcacaaa actgaacacc gtttcggcgg attgccgcag
      541 gagaacagca tggacacgaa caagaccaag gcgttgaaca tggccctggg acagatcgaa
      601 aagcagttcg gcaagggctc ggtcatgcgc ctcggcgatg ccggggtggt gcagcaggtg
      661 gaggcggttt ccaccgggtc gctgggcctg gatatcgccc tcggcatcgg cggtctgccg
      721 cgtggccggg tggtcgagat ctacgggccg gaatcctcgg gaaaaacgac cctggccctg
      781 tccgtggttg cccagatcca gaaactgggc ggggcagcgg cttttatcga cgcggaacac
      841 gcgctggacc ctctctatgc caagaaactc ggcgtgaaaa tcgatgattt gctggtgtcg
      901 cagccggata ccggcgaaca ggccctggaa atcgccgata tgctggtgcg ttccggcggt
      961 gtggacgtgg tggtgatcga ctcggtggcg gcgctcacgc ccaaggccga aatcgaaggc
     1021 gaaatgggcg actcgcacat gggcctgcag gcgcgcctga tgagtcaggc gctgcgcaag
     1081 ctcaccgcaa acatcaagcg ttccaacacg ctggtcattt tcatcaacca gatacgcatg
     1141 aaaatcggcg tcatgttcgg caatcccgaa accaccaccg gcggcaacgc gctcaagttc
     1201 tacgcctcgg tgcgccttga catccgccgc atcggcgcca tcaagaaagg cgaggaagtc
     1261 atcggcagcc agacgcgcgt gaaggtggtc aagaacaagg tcgcgccgcc gttccgccag
     1321 accgaattcg acatcattta taacgaaggc atctcgaagg aaggcgagct catcgacatc
     1381 gggtccgaga gcggcatcgt cgagaagact ggcgcctggt acagcttcaa caaggagcgc
     1441 atcggccagg ggaaggacaa cgcgcgccag ttcctcaagg acaacccgga catcgccgcc
     1501 gagatcgaga aacgcatccg cgagaaggcg gtcgtcgcca cccggtccgc ggctgcggaa
     1561 gacgaggctg aggaagccac ggcggaggaa gaggcctgag gcgggaaacg ccggaaatcg
     1621 atctcccgcg ggcgcgccag ttggccatgc aatggctcgc gcggcgcgag cacagccgtc
     1681 gcgagttgca ggataaatta ttaaagaagg ggtgcggcgc agcactggcc gcggaagttg
     1741 tcggccagct ggaaagcgag catctggttt ccgatgaccg cttcatggaa agcctgattc
     1801 gggcacggcg cagccgcggt tacgggccgc tgcgcattca gaaagaatta caggaaaaag
     1861 gggtggcgcc ggaggcgatc tcggactggc ttgatgccac gagccgggac tggatcgacg
     1921 atatccggcg ggtacaacgc aagaaattcg gggcacgttt acccaaaaat tatactgagc
     1981 gtgcgcgtca ggcgcgcttc ctgcaatatc gcggcttcac ttatgatcaa atacagcaat
     2041 tactcaatcc tcgtggacag gattagcctt cacgcgtcat gaaaagcagc gaaatccggc
     2101 aacgcttcct cgattttttc aagcgccacg gccatacgat cgtgccgtcc agcccactgg
     2161 tgccggcgaa tgacgcgacg ctgctgttca ccaatgccgg catggttcag ttcaaggaag
     2221 tctttctcgg tctcgaaaaa cgtccttatg tgcgcgcggt cagctcgcaa cgctgcgtgc
     2281 gtgccggcgg caagcacaac gatcttgaaa acgtcggcta caccgcgcgc catcacacct
     2341 tcttcgagat gctcggcaat ttcagcttcg gcgattactt caagcgcgaa ggcattcgct
     2401 atgcctggga gttcctgacc caggacctca agctaccccc cgagcggctg tgggtaacgg
     2461 tatataagga tgaccacgag gccgccgaca tctggctgaa ggagatgaag gtggacccga
     2521 agcgtttcgc gtatctgggc gaaagctcca atttctggca gatgggcgat accggccctt
     2581 gtggcccctg ttccgagatt ttttacgacc atggccccgg cattcccggc ggcccgcccg
     2641 gctcgcccga cgaagacggc gaccgctacg tcgaaatctg gaacctggtg ttcatgcagt
     2701 acaaccgcga cgcgagcggc aagctcacgc cgctgcccaa gccctcggtc gataccggca
     2761 tgggcctgga acgcatttca gcggtgcagc agggcgtgca cagcaattac cagatcgacc
     2821 tgttccagaa cctgatccgt gccgcgatcg agatcaccgg cgcaagcgat catcaaagcc
     2881 attcactgaa cgtggtggcc gatcacatcc gtgcggcgac gttcctcatt atcgatggcg
     2941 tgctgccgtc gaatgaaggc cgtggctacg tgttgcggcg catcatccgc cgtgccattc
     3001 gtcacgggca ccgcctcggc gcgactgaga catttttcta taaactggtt ggcccgctgt
     3061 gccagcagat gggcgatgcc tatcccgagc tcaacaagga gcgcgcacag gtcgaacgcg
     3121 tgttgcagca ggaagaagat cgttttaacg aaaccctgga tcagggcctg aagattctgg
     3181 aagacgggat tgccgggctc aagggcgaca ccattccggg cgagctgatt ttccggctgt
     3241 atgacaccta cggatttccg gtggacctca cgcgcgacgt ggccatggaa cgcaatctca
     3301 aaatcgacat ggcgggtttc gagcgcgaga tggaagcaca acgcgagcgc gcgcgtgctg
     3361 cctcgtcgtt taaaaccgta gagatcggtg ccgtgaccgt ggaccagccg acgcaattca
     3421 ccggctatga acgcttgagc gatgagggaa aaatcctgca aatcacgcgt gcttccgacc
     3481 tcgcccagac ggacagtctg cgtgctggtg aatcgggaaa tatcgtactc gaccgcacgt
     3541 cattttatgc cgaatccggc ggccaggtgg gcgaccgcgg cgtgttgcgc gtaggcgacg
     3601 ccatatttcg cgtggaagac acgcagaaac acggacagat ccatgtccat cagggacggg
     3661 tggagaaggg ccagtttcgc gcgggtgaca aggtaacggc cgaggtcgat gccgtcttgc
     3721 gccgcgcgac catgctcaat cactcggcca cgcatctgat gcacaaggcg ctgcgcgacg
     3781 tgctcggtgc gcatgtgcag cagaagggct cgctggtgga cgatcagcac acgcgtttcg
     3841 acttttccca tttt
//
LOCUS       DBNGZE010000254         3935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_313669, whole genome shotgun sequence.
ACCESSION   DBNGZE010000254 DBNGZE010000000
VERSION     DBNGZE010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_313669"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..555
                     /locus_tag="AC16FV_06590"
     CDS             184..555
                     /locus_tag="AC16FV_06590"
                     /inference="COORDINATES: protein motif:HMM:NF045049.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2849760.1"
                     /translation="MAIKKCTTCHKPLRGRQRKFCSRRCKNTDTNNKHQSYQAQQLRG
                     RERKLELIRLKGAKCEYCGYKKNYAALEFHHPNPHEKDFQLDIRTLSNRRWEAVMIEA
                     RKCLLLCSNCHAEEHNPDCVM"
     gene            complement(628..2484)
                     /gene="rpoD"
                     /locus_tag="AC16FV_06595"
                     /pseudo
     CDS             complement(628..2484)
                     /gene="rpoD"
                     /locus_tag="AC16FV_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(2586..>3935)
                     /gene="dnaG"
                     /locus_tag="AC16FV_06600"
     CDS             complement(2586..>3935)
                     /gene="dnaG"
                     /locus_tag="AC16FV_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR2849761.1"
                     /translation="RGITGEIARDFDLGFAPDGWDNLLRALGKDDATREALARAGLVV
                     KKDSGGYYDRFRDRVMFPIEDHRGRIVAFGGRVIDKGEPKYLNSPETPLFHKGRELYG
                     LFHARDAIKRENRVMVVEGYMDVVALAQFGIDFAVATLGTATTRDHLERLFRHAPEVV
                     FCFDGDRAGREAAWRALDNALPVLREGRQISFLFLPEGEDPDTLVRKEGRDAFLARLK
                     KALPLPDYLFDTLSRQVDLGRLDGRARLVELARPLLSKMPAGVLRQMMLDRLAELSRT
                     DPGNLPVSAGNPDQNATPREYRTASSGPREPPSMVRLAIAMLLQRPDLARNVHNANAL
                     AELDMPGIPLLVELLRVVKSTAEMNIASIIENFSDSEHKQNLAKLAFWQHPALEQDLE
                     TEFSGVMSRLEGALARQKTEQLLQKRQTGGLNQEEKDELARLLSLKRDNSRVSPTRH"
BASE COUNT          760 a         1162 c         1117 g          896 t
ORIGIN      
        1 tcggcatgaa cgcacctttg tgatgagtcc cctgctctaa ccaactgagc tacaggccca
       61 acgccgacat tctatcagct ttgagaatac caagtaccta tctgcaagat gacagtagac
      121 ctttagcgcc tcactctgta gattattaaa aataaaataa tctgtattta taaaggtcct
      181 cagatggcta taaaaaaatg cacgacatgt cataagccac tacgggggcg tcaaagaaaa
      241 ttttgttccc gtcgatgcaa aaataccgac accaacaata aacaccagtc ctatcaagcg
      301 cagcagcttc gcggacgcga acggaaattg gaactcattc gcttgaaagg agcaaaatgc
      361 gaatattgcg gttacaaaaa gaactacgcc gcactcgaat ttcaccatcc caaccctcac
      421 gaaaaagatt ttcagctgga tattcggact ctgtcgaacc gtagatggga ggcagtcatg
      481 attgaagcca ggaagtgtct cctgctttgc tcaaactgcc atgctgaaga acacaatcct
      541 gattgtgtta tgtgaccaca ctattgtcag ccagtcagaa acaaaaaggg cccagtaagg
      601 gccctttttg ttatgcggca ttataaatta gtcgaggaag ctgcgcagat gctcggagcg
      661 actcgggtgg cgcagcttgc gcagggcctt ggcttcgatc tggcggatgc gctcgcgcgt
      721 gacgtcgaac tgcttgccga cctcttccag cgtgtgatcg gtgttcatgc cgatgccgaa
      781 gcgcatgcgc aacaccttgg cctcgcgcgg tgtcaacgtc tcgaggatct ccgaggtcgc
      841 gaacttgagg ccgctgttgg tggcggcgtc cggcggcgcc acggaattgg cgtcctcgac
      901 gaaatcgccg agatgcgaat cctcatcgtc gccgatgggc gtctccatgg agatcggcta
      961 tttcgcgatc ttgagcacct tgcggatctt gtcctcgggc atgtccatac gctcggccag
     1021 ttcctcgggc gtggcctcgc ggcccatttc ctggagcatc tggcgcgaaa tgcggttgag
     1081 cttgttgatg gtctcgatca tgtgcaccgg gatgcggatg gtgcgcgcct ggtcggcgat
     1141 cgagcgcgtg atcgcctggc gaatccacca cgtcgcatag gtcgagaact tgtagccgcg
     1201 gcggtattcg aatttgtcca ccgccttcat caggccgata ttgccttcct ggatgaggtc
     1261 gaggaattgc aggccgcggt tggtgtattt cttggcgatc gaaatcacga ggcgcaggtt
     1321 ggcctcgacc atttctttct tggcgcggcg tgccttggcc tcgccaatgg acatgcggcg
     1381 attgacttcc ttgagctcgg aaatcggcag gcccgcgcgc tcctcgagct tcttgagctt
     1441 gtcctgggcg gcgtggatgg cgtcggtatt gttttcgatg accgaggcat tgccctcgcc
     1501 gcacttgacg gcctttttca cccagcgctt attggtctcg tggccgggga aaatcttgat
     1561 gaaggtcttg cgcggcatgc gcgccttgct gacgcagatg tccatcacga ccttttcctg
     1621 gccgcgcgca tattccacca gcgagcgcac gtattcgacc aagcggttga tctgcttgga
     1681 aacgaacttg atctgcatga attcgtcagc gaccctggcc tgaacccgct tgacctcggg
     1741 gctgtcgtaa ccctttttct cgatggcctt gagcaggttc gtgtgcagct tgcggatgcg
     1801 tgcgaagcgg atcagcgcct cttcccggtt cgggccggtt tcgtcgtcgc tgtcgctgct
     1861 ggaaccgctc tcgtcgtcat ccttctcgtc gtcgacatcg gccgcggcct tcacggccgg
     1921 agcctcgggc aggggcacgt caaccgcgtt tggatcgacg aaatccacta ctaggtcggt
     1981 caggcgcatc tggtcgatct cgaccaggtc catcatgcgc acgacttccg cgatcgcggc
     2041 cgggcaggcg gcaatggcct cggtcgtctg gcgcacgccg tcttcgatgc gcttggcgag
     2101 atcgatctcg ccttcgcgcg tgagcagttc caccgtgccc atctcgcgca tgtacatgcg
     2161 caccgggtcg gtggtgcgtc cgaattcgct gtccacggcg gaagtcacca cctgttccgc
     2221 ctcttccgca gcttcctctt cgtcttccgc cggcggcggg gtttcggtgg ttttgagcaa
     2281 cagggtgtcc gggtccggcg cctcgtcgta caccgcgatg cccatgtcgt tgatcatgtt
     2341 caccaccgcc tcgatctggt cggtgtcgtg aacgtcttcg ggcagatggt cgttgatctc
     2401 acgatacgtg agaaaaccct gctccttgcc ctgaatgatc agttttttga gctgtgaccg
     2461 ttgtgtttcc tgatccattg ccattgatcg ataacctgtg tcgccgtcta aggcgttgtg
     2521 ttgaaacgta aaagcccgcg aaaaacgcga gagtatagct taagtggttg cttgccccaa
     2581 acttttcaat ggcgagtagg tgatacgcgg gaattatctc tcttgaggct caataatcgt
     2641 gccaattcgt ccttttcctc ctgattgagc ccgccagtct gtcttttttg caatagctgt
     2701 tcggtttttt gtcgggccaa tgccccctcc agacgactca taacaccgct aaattcagtt
     2761 tcaaggtcct gttccagggc cggatgctgc cagaacgcca gttttgccaa attttgtttg
     2821 tgctcactat cactaaaatt ctctattata gacgctatat tcatttctgc tgtcgattta
     2881 accactctca gtaattccac caataacggg atccccggca tgtccagctc agccaaggca
     2941 ttcgcattgt gcacatttcg cgccaaatca gggcgctgca acaacatggc aatggccagc
     3001 cggaccatgg aaggcggttc gcgcggaccg ctgctggcgg tacggtattc acgcggtgtt
     3061 gcattctggt ccggatttcc ggcactcacc ggcaaattac ccgggtcggt gcggctcagc
     3121 tccgccagcc gatccagcat catctgacgc aagacacccg ccggcatctt ggacaagagc
     3181 ggacgggcaa gttccaccag gcgcgcgcgc ccatcgagac ggcccaggtc cacctggcgc
     3241 gacagggtat cgaaaaggta gtccggcaat ggcagcgcct ttttgaggcg ggccaggaac
     3301 gcgtcacggc cctccttgcg caccagcgtg tccggatcct cgccttccgg aagaaacagg
     3361 aaactgatct gccgtccctc gcgcaacacc ggcagggcat tgtccagcgc gcgccaggcg
     3421 gcctcgcggc cggcgcggtc gccatcgaag caaaaaacca cctccggagc gtggcgaaac
     3481 aggcgctcca gatgatcgcg cgtggtggcg gtgccgagcg tggccaccgc gaaatcgatg
     3541 ccgaactgcg ccagcgccac cacgtccatg tagccctcga ccaccatgac gcggttttcg
     3601 cgcttgatgg cgtcacgtgc atgaaacagc ccgtagagtt cacgtccctt gtgaaacagc
     3661 ggcgtctccg gggagttgag gtatttgggc tcgcccttgt cgatcaccct accgccgaag
     3721 gcgacgatac gtccgcgatg gtcttcgatc gggaacatga cgcggtcgcg gaagcggtcg
     3781 tagtagccgc cgctatcctt tttcacgacg aggccggcac gcgccagcgc ctcgcgagtc
     3841 gcgtcgtctt ttcccaaggc gcgcagcagg ttgtcccagc cgtcgggggc gaagccgagg
     3901 tcgaagtcac gcgcgatctc gccggtgatg ccgcg
//
LOCUS       DBNGZE010000255         4611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_451742, whole genome shotgun sequence.
ACCESSION   DBNGZE010000255 DBNGZE010000000
VERSION     DBNGZE010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_451742"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="AC16FV_06605"
     CDS             <1..379
                     /locus_tag="AC16FV_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849762.1"
                     /translation="EFRMALLDLGLVERRQSGTTSAIPGMRRKHRDTEGSPRRIWGGA
                     WLDFSMLAAALLLALTLGIFPASLGPTRNGADEMTRAQMTWKKPQPARKVIEVSRETE
                     ADAPPKVIERKDKYDALRQAWGG"
     gene            405..989
                     /locus_tag="AC16FV_06610"
     CDS             405..989
                     /locus_tag="AC16FV_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849763.1"
                     /translation="MKLPQRGLSSSHRLVLILAGCVQLISGCASFREMTCAQFEESRQ
                     EADYATRYLFMETESETAARNFKPLPKNASAIVRQYKMRVEPSAIKPCNHLTIHKDIY
                     LQRKMDRNLVLEEVREFYTSDGALIAAKTESIADQLRTSGFYSGSALLPIPEKAPPGK
                     YRLVSKLMLKRKNNSHVTRLAKTSVNFQVVTRKK"
     gene            complement(993..1649)
                     /locus_tag="AC16FV_06615"
     CDS             complement(993..1649)
                     /locus_tag="AC16FV_06615"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR2849764.1"
                     /translation="MFTGIIQAVGKIAAIEQKGGDARLRVDTGGLDMSNVNTGDSVAV
                     SGVCLTAVEHSASGFTADVSGETLSRTTLGQRRVSDAVNLEKALTLGTPLGGHLVSGH
                     VDGVGTVVSREAEARSVRFRFQAPDTLARYIAEKGSICVDGVSLTVNAVNGAQFDVNI
                     VPHTLAQTTLDSFRPGSKVNLEVDLLARYLERLMLGEGAAKSGSTVTREFLAQHGFLK
                     "
     gene            complement(1652..2758)
                     /gene="ribD"
                     /locus_tag="AC16FV_06620"
     CDS             complement(1652..2758)
                     /gene="ribD"
                     /locus_tag="AC16FV_06620"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR2849765.1"
                     /translation="MTTSAADARFMARALQLARRGMYTTDPNPRVGCVIVKDGKIIGE
                     GWHERAGEPHAEINALNQAGKMNTHSAGVYLTLEPCCHQGRTPPCTQALIKSGVRRVV
                     VAMRDPNPLVAGKGLKEIESHGIQTDVGLMEAEAATLNPGFISRMKRGRPFVRVKLAA
                     SLDGRTGMANGDSKWITSEAARADVQKWRARSSAILTGVSTVLADDPQLNVRDFKTGR
                     QPLRVVLDSRLRMPPRARMLRGEGETLIVTASEDVALAETLKKAGAEVAHISTPQKTV
                     DLAGLLKHLAWLEVNEVLVEAGATLCGALLRAGVVDELILYYAPHIMGVHERGMFALP
                     PLLRMSDRINVEITDVRAVGKDWRVMAKVSEKQD"
     gene            complement(2755..3309)
                     /gene="nrdR"
                     /locus_tag="AC16FV_06625"
     CDS             complement(2755..3309)
                     /gene="nrdR"
                     /locus_tag="AC16FV_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR2849766.1"
                     /translation="MRCPFCSADETRVVDSRLSDDGDSVRRRRECEVCGERFTTFERA
                     ELRLPQVIKSDGRREPFNEKKLNNGLSRALEKRPVDTDAVEKIIGRIRHKLMASGEGE
                     VPSRQIGEWIMEELKELDPVAYVRFASVYRSFDDLNAFRDEVQRLQNEPSAELRRKQL
                     SLIPEPEPPAKPATGARKSPGKKS"
     gene            complement(3448..4161)
                     /locus_tag="AC16FV_06630"
     CDS             complement(3448..4161)
                     /locus_tag="AC16FV_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849767.1"
                     /translation="MAIKKALGRMDYATDGGNQLAGAMVSDSDILWGTCGGSASYSVT
                     ADDVTGDFNGTINFSGYCDDGDTLTGGADFSGNIDINTLAFNSINLSTSSLTITSGGD
                     TFAVAGSFSFTSVMAGSETLLMTLNIRDAAGTVYRIENLQCAITYDSPTFGTDRIRIV
                     SGRYFHPVYGYVDVITTSDFLINSDYPYAGVLLVTGSGGSKAELTANSDAQTFSVMVD
                     ADGIGGYDGPATTYRWDSI"
     gene            complement(4183..4449)
                     /locus_tag="AC16FV_06635"
     CDS             complement(4183..4449)
                     /locus_tag="AC16FV_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849768.1"
                     /translation="MKISKLGDVEQYRLTSLIFIAFVLAACSGGGGGGSDSSSAVNYT
                     GATSQAVVTNSNGDDLSAGAALGVGTGSSTSLVGTATSEVNPPA"
BASE COUNT          957 a         1399 c         1326 g          929 t
ORIGIN      
        1 ggaattccgc atggcgttgc tggatctggg gctggtggag cgcagacaat ccggcactac
       61 cagcgccatt ccgggcatgc gccgcaaaca ccgcgacacc gaaggctctc cgcgccgcat
      121 ctggggcggg gcttggctgg atttttccat gctggcggcg gcgttgctgc tggcgctgac
      181 gctgggaatt ttccccgcga gtctcgggcc gacgcggaac ggcgccgatg agatgacgcg
      241 cgcgcagatg acatggaaga agccgcaacc cgccagaaag gtaatagagg tatcgcgcga
      301 aacagaggcg gacgcgccgc caaaggttat cgagcgcaaa gacaagtacg acgcgctgcg
      361 ccaggcctgg ggcggctgaa caacaatctc gccagggacg ggccatgaag ttaccgcaac
      421 gcggtttgtc ctccagccac cgattggtat tgatcctcgc cggttgcgtc cagctgattt
      481 cgggctgcgc gagtttccgc gagatgacct gcgcgcaatt cgaggaaagc cgccaggaag
      541 ccgactacgc cacgcgctat ctctttatgg agacggaatc ggaaacggcg gcgcgaaact
      601 tcaagccgtt gcccaaaaac gccagcgcca tcgtgcgcca atacaagatg cgcgtggaac
      661 cctccgcgat aaaaccctgc aatcatctga cgatccacaa ggatatttat cttcagcgca
      721 agatggacag gaatctggtg ctggaggaag tgcgggaatt ttatacgtcc gatggcgcac
      781 tgatcgccgc taaaaccgaa tcgatcgccg atcagttgcg caccagcggt ttctattccg
      841 gaagcgccct gctgccgatc ccggaaaaag ccccgcccgg aaaataccgc ctcgtcagca
      901 agctgatgct aaaaaggaaa aacaactcac acgtcacgcg gcttgccaaa accagcgtaa
      961 actttcaggt agtcacgcgt aaaaaataat tcttacttga gaaagccgtg ctgggcgaga
     1021 aattcgcggg tgacggtcga gccgctttta gccgcgccct caccgagcat gagccgctcg
     1081 agatagcgcg ccagcagatc aacttccaga ttcacttttg agccgggccg gaaactgtcc
     1141 aacgtggttt gcgccagcgt gtgcggcacg atgtttacat cgaattgcgc accgttgacc
     1201 gcgttgaccg tgaggctcac gccgtccaca cagatggaac ccttctcggc gatatagcgg
     1261 gccagtgtat ccggcgcctg gaaccggaac cgcaccgatc tggcttcggc ttcacggcta
     1321 accactgttc ccaccccatc cacatggccg ctgaccagat gccctccgag cggagtgccg
     1381 agcgtcaggg ccttttccag gttcacggca tcgctgacac gtcgctgtcc cagcgtcgtg
     1441 cgggaaagcg tttcccccga gacatcggcg gtgaagcccg atgcggaatg ttccacggcc
     1501 gtcaggcaca cgccgctgac ggcgacgctg tcgccggtat taacgttcga catgtccagc
     1561 ccgccggtgt ccacgcgcag gcgcgcatcg ccgccctttt gctcgatggc ggcgattttt
     1621 cccacggcct gaatgattcc ggtgaacatt tctagtcctg tttttcgctt actttcgcca
     1681 tgacacgcca gtctttcccc accgcccgca catccgtgat ttccacgtta atgcgatcgg
     1741 acatgcgcaa cagcggcggc aacgcgaaca tgccgcgctc gtgaacgccc ataatatgcg
     1801 gggcgtaata gagtatcagc tcatccacca ccccggcccg cagcagtgcg ccgcacaggg
     1861 tggcgccggc ctcgaccagg acttcgttca cttcgagcca cgccagatgt ttgagaaggc
     1921 cggcaaggtc cacggtcttc tgcggcgtgg agatatgcgc cacctcggcg ccagccttct
     1981 tcagagtttc cgccaaggcc acgtcttcgc tggccgtcac gatgagcgtc tcgccctcgc
     2041 cacgcagcat gcgcgcccgg ggcggcatgc gcaggcgact gtccagcacc acgcgcagcg
     2101 gctggcgtcc ggtcttgaaa tcgcgcacgt taagctgagg atcgtccgcc agcacagtgc
     2161 taacgccggt caggatcgcc gagctgcgcg cgcgccattt ctgcacgtcg gcgcgcgccg
     2221 cctcgctggt gatccatttg ctgtcgccgt tcgccatgcc ggtgcggccg tcgagactgg
     2281 cggcgagttt cacgcgcacg aagggccggc cgcgtttcat gcggctgatg aatccggggt
     2341 tcagtgtcgc cgcctccgct tccatcagtc cgacgtccgt ctggatgccg tgactctcga
     2401 tctccttgag ccccttgccc gccaccagcg gattgggatc gcgcatggcc accaccacgc
     2461 gccgcacgcc cgacttgatc agcgcctggg tgcagggtgg cgtacgcccc tgatggcagc
     2521 agggttccag cgtcaggtaa acaccggcgc tgtgggtgtt catcttgccg gcctgattga
     2581 gcgcgttgat ctcggcatgc ggctcgccgg cgcgctcgtg ccagccttcg ccgatgatct
     2641 tgccgtcctt gacgatcacg cagccgacgc gcggattggg atcggtcgta tacatgccgc
     2701 ggcgcgccaa ttgcagcgcg cgcgccatga aacgcgcatc ggcggcggag gtagtcatga
     2761 tttctttccg ggagacttgc gggcgccggt ggccggcttg gccggcggtt ccggttcggg
     2821 gatcagcgaa agctgcttgc ggcgcaattc ggcgctgggc tcgttttgca agcgctgcac
     2881 ttcgtcgcga aaggcgttca aatcgtcgaa gctgcggtaa accgaggcaa agcgcacata
     2941 ggccaccggg tcgagctcct tcagttcttc cataatccac tcgccgatct ggcgcgaagg
     3001 cacttcgcct tcgccgctcg ccatcagttt gtggcggata cgcccgataa ttttttcaac
     3061 cgcgtcggta tccaccgggc gtttttccaa cgcgcgcgaa agaccgttgt tcagcttctt
     3121 ttcattgaag ggttcgcgcc ggccgtcgga cttgatcacc tgcgggagtc gcagctcggc
     3181 gcgctcgaag gtggtaaagc gttcgccgca cacctcgcat tcgcgccgac ggcgcacgga
     3241 atcgccgtcg tcggacaggc gtgaatccac gacgcgggtt tcatcggcgg aacagaacgg
     3301 gcagcgcata gatcgtatcg cggacaaaat accgcgccat tgtcactttt cagcggtata
     3361 tcttaaatca gcgtccagga aatgaggacc gcgccagatt ggtaatccag cctgacgcca
     3421 ggctatatcc ggctgaatgg cgtgcaatca gatcgaatcc cagcggtaag tggtcgccgg
     3481 accgtcatat ccgccaatac cgtcggcatc caccatgacc gagaaggtct gtgcatcact
     3541 gttcgcggtc agttcggcct tgctgccacc actgcccgtc accaggagca cgccagcata
     3601 aggataatct gaattaatca gaaaatccga cgtggtaatg acatcaacat atccgtatac
     3661 cggatgaaag tatctgcctg acacaatgcg gatccgatct gtaccgaagg ttggcgaatc
     3721 gtatgttatc gcacattgca gattctcaat acgatacacg gtcccggcgg catcccggat
     3781 gtttaacgtc atgaggagag tttccgagcc agccatgacg ctggtgaatg aaaacgagcc
     3841 tgcaacagcg aaagtatcgc ctcccgaagt gatcgtcaga gaggaggtgg acaaattaat
     3901 ggaattgaaa gcaagcgtat ttatatcgat gtttcccgaa aaatccgcgc cccccgttaa
     3961 agtatcgccg tcatcgcaat agccgctgaa attgatggtt ccattgaagt cccccgtcac
     4021 atcatccgcg gtcacgctat atgacgcgct cccgccgcag gtgccccata aaatatctga
     4081 atcagaaacc atcgcgccgg caagttgatt tccgccgtct gtcgcgtaat ccatgcggcc
     4141 gagcgctttt ttgatcgcca tggaggctga caaggatcgt agctaggcgg gaggatttac
     4201 ttcactggtg gcggtaccga caagtgaagt cgatgaacct gttcccacgc cgagagcagc
     4261 gccggcgctg agatcatcac cattggaatt tgtaactaca gcctgggatg tggcgccagt
     4321 gtaattgacg gcgcttgagc tgtcgctgcc gccgccaccg ccaccgctgc aagccgccag
     4381 gacgaaagca atgaatatca gacttgtcag tcgatattgt tcgacgtcgc caagtttgga
     4441 aattttcatg ttccttcctc cattattatt cgtatgaaac tacgaaggcc tttaagccgg
     4501 tataccggat gaatatttta tacctcgcct gaattttaat ctccatttaa acgaaacggc
     4561 ggcttgaaaa ggtatttttt aatgttgata tacggggaag cgatggcaaa c
//
LOCUS       DBNGZE010000256         2931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_522660, whole genome shotgun sequence.
ACCESSION   DBNGZE010000256 DBNGZE010000000
VERSION     DBNGZE010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_522660"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16FV_06640"
     CDS             complement(<1..310)
                     /locus_tag="AC16FV_06640"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR2849769.1"
                     /translation="MSGNSIGKLFTVTTAGESHGAAYVAIVDGCPPGMALSEADIQPD
                     LDRRKPGKSRHTTQRRESDEVKILSGVFEGKTTGTPIGLLIQNTDQRSQDYSKILDRF
                     R"
     gene            complement(350..664)
                     /locus_tag="AC16FV_06645"
     CDS             complement(350..664)
                     /locus_tag="AC16FV_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849770.1"
                     /translation="MTPRTRDEFLDLVDQLIFEIEEVLMCAQDEGDPEDYEFSELLPV
                     YEQLSGDLKKLHAEVMQGKHVFGADQDLPFMPLVNKWKERMPFHHLFATLNAVNKQGL
                     PG"
     gene            complement(822..1634)
                     /locus_tag="AC16FV_06650"
     CDS             complement(822..1634)
                     /locus_tag="AC16FV_06650"
                     /inference="COORDINATES: protein motif:HMM:NF018352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HIR2849771.1"
                     /translation="MRQILIPSLIITLCLLVPSARAGDLPRWELGLGLAGLTIPDYRG
                     SDEQRGYLLPLPYVQYRGEIFQIDREGAHGKLFDSKRVRLELSAAAGPPARSRDNEAR
                     RDMPDLDPTVELGPSLEIYLTRDAARERAWLIRLPWRAVVATDLSHADGIGWVFAPSL
                     NYETRNNGGWGMGVAAGPLYASEKYHDYYYEVAPAYATPTRPVYDAPGGYSGSRLTFT
                     ASRRFPDFWVGAFARYDRLSGVAFEDSPLVKKKSSFMAGVGIAWVLAEAKKY"
     gene            1699..2658
                     /locus_tag="AC16FV_06655"
     CDS             1699..2658
                     /locus_tag="AC16FV_06655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR2849772.1"
                     /translation="MRRHSAIRPASFTTGFHSFIRLFLVALFVLPLWTGAAAATAAAK
                     SSVSAAEPAARYSKGLLWKIESPGAAANYLFGTIHSDDTRVTALPDAVTRSLDASSRF
                     VMEAIIDGDSLVRMAEVMFFNDERTLEQVIGGKLYAQTVKAMNARGIPTLGVEKQKPW
                     AVMMALSMPPPKTGEFLDIVLQARATQQNKPVSGLETVAEQLAIFDGLPLRDQVALLE
                     DTVRMQADLDKEFEALHKAYLARDLAAVVAISEKHEPGDERLYRDVMDRLLDQRNRRM
                     VERMAPMLKQGGAFIAVGAAHLPGEAGLLYRLEKAGYRVTAVY"
BASE COUNT          596 a          887 c          859 g          589 t
ORIGIN      
        1 ggcggaagcg gtccagaatt ttggagtaat cctgcgaacg ctggtcggtg ttctggatca
       61 gcaggccgat gggcgtgccg gtggtcttgc cctcgaacac gcccgagagg attttcactt
      121 cgtccgattc acgccgttgc gtggtgtggc gcgacttgcc gggtttgcgc cggtcgagat
      181 caggctggat gtcggcctcg ctcagcgcca tgcctggcgg acaaccgtcc acgatggcga
      241 cgtaggccgc tccgtgggat tcgcccgcgg tggtgacggt gaagagcttg ccgatggaat
      301 ttccggacat gttgcgatgt taccggcaaa caggcgaagt cacaaaccgt cagcccggca
      361 gaccttgctt gttcacggca ttgagcgtcg cgaacagatg atggaacggc atccgttcct
      421 tccatttgtt caccagcggc atgaaaggca ggtcctggtc cgcgccgaag acgtgcttgc
      481 cctgcatgac ctcggcgtgc agcttcttga gatcgcccga caactgttcg taaaccggca
      541 gcagttcgga gaattcgtag tcttcgggat cgccctcgtc ctgcgcgcac atcagcacct
      601 cctcgatttc gaagatcagc tgatccacca ggtccagaaa ttcgtcccgc gttctcggtg
      661 tcatcacctc atagagtttg ttaaaaaaat ccttaagtaa catccgaatc cgtcattccg
      721 gcggaagccg gaatccagca tattaaggat ttattgtttt aatatcctgg accccggctt
      781 tcgccggggt gacagacgtt ttttcaaaaa atagaaatcc tttaatactt cttcgcttcc
      841 gccaataccc aggcaatgcc caccccggcc atgaaagagg atttcttctt caccagcggg
      901 ctgtcctcga aggccacgcc ggaaaggcga tcataacgcg cgaacgcccc gacccagaaa
      961 tcagggaagc gacggctcgc ggtgaatgtc aggcgactgc cgctgtatcc gcccggcgcg
     1021 tcgtagacag ggcgcgtggg cgtggcgtag gccggcgcga cttcataata gtaatcatga
     1081 tacttctccg aggcgtacag cgggccggcc gcgacaccca tgccccagcc tccattattc
     1141 cgcgtctcgt aattcaggct cggcgcgaac acccagccga tgccgtcagc atgcgacaga
     1201 tcggtggcga cgaccgcgcg ccatggcagc cggatcagcc acgcgcgttc gcgcgccgca
     1261 tcgcgcgtca ggtaaatctc cagcgaggga ccaagctcca cggtcgggtc gaggtccggc
     1321 atgtcgcggc gggcctcgtt gtcacggctg cgggccggcg ggccggcagc ggcgctcagt
     1381 tccaaccgaa cgcgctttga gtcaaacagc ttgccatgcg cgccttcgcg gtcgatctga
     1441 aaaatctcgc cccggtactg cacatagggc aagggcaaaa gatagccgcg ttgctcgtcg
     1501 gacccgcggt agtcggggat ggtcaaaccg gccaggccca agcccagttc ccagcgcggc
     1561 aagtcgccgg cgcgcgcaga cggcacgagc agacaaagcg ttattattaa ggaaggaata
     1621 agtatttggc gcattggcat tcctaatata ttcttggata catggtaatc gggcccattt
     1681 cctgaagcca tccgttcgat gcgacgacat tccgccatcc gcccggcgtc gttcacgacg
     1741 gggttccatt cctttataag acttttcctt gtcgcccttt ttgtcctgcc gctctggacc
     1801 ggcgcggcag cggcaaccgc cgcggccaag agcagcgtca gcgccgccga accggccgca
     1861 cgttacagca aggggctgct gtggaaaatc gaatccccgg gcgccgcggc caattacctg
     1921 ttcggcacga tccactccga tgacacgcgc gttaccgccc tgcccgatgc cgtaacccgc
     1981 tcgctggatg cctcgagccg cttcgtcatg gaagcgatca ttgacggcga cagcctggtg
     2041 cgcatggcag aggtcatgtt cttcaatgac gagcgcacgc tggaacaggt catcggcgga
     2101 aaactctacg cgcaaaccgt gaaggccatg aacgcgcgcg gcattccgac gttgggcgtc
     2161 gagaaacaga aaccctgggc ggtgatgatg gcgctgtcca tgccgccgcc caagaccggc
     2221 gaattcctcg acattgtgct gcaggcgcgc gccacgcagc agaacaaacc cgtctccgga
     2281 ctggaaaccg tcgccgaaca gctggcgata ttcgacggac tcccgctgcg cgatcaggtg
     2341 gcgttgctgg aagacacggt acgcatgcag gcggacctcg acaaagaatt cgaggcgctg
     2401 cacaaggctt acctcgcgcg cgatctggcc gcggtcgtgg caatcagcga aaaacacgaa
     2461 ccgggcgacg agcgtttgta ccgggacgtc atggaccggt tgctcgatca gcgcaaccgg
     2521 cgcatggttg agcgcatggc gccgatgctc aagcaaggcg gagctttcat tgccgtgggc
     2581 gcggcgcacc tgccgggcga agccggcttg ctgtaccggc tcgaaaaggc cgggtatcgt
     2641 gtcaccgccg tttattaata atttaatccg ctgattaacg cagatcctaa aaagatgaaa
     2701 gcagataaaa caaaaactcg cgtgatctat ctgcgtttat catcatttat catccgcgtt
     2761 catctgcggt tgccttgtat ttttttagcg aagcttcatg ctgaatatcc ctatcaagga
     2821 tcagttccgg gaaagccggg tttttaacgc acggctgacg gtggtcggcg ccatcgtcct
     2881 ggcgctgatc ctggcgctgt tcctgcgcct cgtctatctc cagatcttcg c
//
LOCUS       DBNGZE010000257         2815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_244922, whole genome shotgun sequence.
ACCESSION   DBNGZE010000257 DBNGZE010000000
VERSION     DBNGZE010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_244922"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="holA"
                     /locus_tag="AC16FV_06660"
     CDS             complement(<1..830)
                     /gene="holA"
                     /locus_tag="AC16FV_06660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR2849773.1"
                     /translation="MQINADQLVNHLKRGLAPVYFVYGEELLLVEESCQAIRDAAQAN
                     GYLDRQSLTVESGFDWNGLFASTQSLSLFSERRLIELRLPTGKPGEAGAKILVEIATQ
                     PPADTVLLVSCGKLDKATREAKWAKALEGAGTVVVAWPLEAAQWPVWIRRRMEAKGLK
                     PGPGVVDLLAHLMEGNLLACAQEIDKFSLRFSGGEVGLDDIEDNLSDNARYNVFALAD
                     TCLRGETATVERILNNLRGEGVEPILVLWALAREARELAQMASLVASGQPVMRVLDAR
                     R"
     gene            complement(814..1254)
                     /locus_tag="AC16FV_06665"
     CDS             complement(814..1254)
                     /locus_tag="AC16FV_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR2849774.1"
                     /translation="MPPPLSTLEVSVEGNQMENNPLLAAMKNALRSQTDVQLQELGEA
                     PRLMLYGEQSDSQVLSVTSTGKVDEYLLKYEVSFRLINKDGKLLAPPQTVKVQRDHQF
                     DRLNVLAKEREEQELRREMQRDAVQQILRRLSRITLVNPDANQR"
     gene            complement(1404..>2815)
                     /locus_tag="AC16FV_06670"
     CDS             complement(1404..>2815)
                     /locus_tag="AC16FV_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR2849775.1"
                     /translation="AVDAIAADLKAKGLGDKQVMWRLRDWGISRQRYWGCPIPIIHCD
                     RCGDVPVPDKDLPVVLPEDCVPDGTGNPLNKRADFVNCQCPKCGKAARRETDTMDTFV
                     DSSWYYARYASKRTNAAMVDGETSYWMPVDQYIGGIEHAILHLLYSRFWWKVMRDIGL
                     VRGDEPFMRLLTQGMVLNEIFFRKPTSGRIQYFNPAEVEIKTDDKGGRLGTVLKSDGQ
                     PVESGGIGTMSKSKNNGVDPQSLIEQYGADTARLFMMFAAPPEMTLEWSDEGVAGASR
                     FLRRLWKLVAEHANQGLVTRGDFSSLSPALKDMRRQAHQTLSKVTDDIGRRRTFNTAI
                     ASVMELLNSLARLDDNSASAKAVRQEALEFAVLTLSPIVPHICHALWQALGHKEAVID
                     VRWPKPDDAALKQDNLEIVVQVNGKLRGRVSVPVQAGEDKVRELALADEAVARHVGGK
                     AVKKVIVVPGKLVNIVVAG"
BASE COUNT          524 a          928 c          802 g          561 t
ORIGIN      
        1 cgtcgcgcat cgaggactcg catcaccggc tgtccggagg cgaccaggct tgccatctgc
       61 gccaattcgc gcgcctcgcg cgccaacgcc cacagaacca gaatgggttc gacgccttca
      121 ccgcgcaggt tgttgagaat ccgctcgacc gttgcagttt caccccgcag gcaggtgtcc
      181 gccagcgcaa aaacattgta acgggcattg tcgctgagat tatcctcgat gtcatccaga
      241 cccacttcgc cgccgctgaa acgcagggaa aatttgtcga tttcctgcgc acaggcgagc
      301 aggttgcctt ccatgagatg cgccagcagg tccaccacgc ccggccccgg cttcaggccc
      361 ttcgcctcca tgcgacgccg tatccagacg ggccactgcg ccgcttccag cggccaggcg
      421 acaacgaccg tgcccgcgcc ttccagcgcc ttggcccatt tggcctcgcg cgtcgccttg
      481 tcgagcttgc cgcaactgac cagtaacacg gtatccgccg gaggttgtgt tgcgatttcg
      541 acgagaattt ttgcgccggc ctcgcccggt ttgccggtcg gcaggcgcaa ttcgataaga
      601 cgccgttcgg aaaagagcga gagcgactgc gtggaggcga acaggccgtt ccagtcgaag
      661 ccgctttcca ccgtcagcga ctgacggtcg agataaccat tggcctgcgc ggcgtcgcga
      721 atggcctgac agctttcctc gaccagcagc aactcctcgc cgtacacgaa gtacaccggc
      781 gccaggccgc gcttgaggtg attgactaat tgatcagcgt tgatttgcat cgggattgac
      841 aagagtgatg cgcgacaacc gtcgcaggat ctgttgcacc gcgtcgcgct gcatttcgcg
      901 ccgcaattcc tgctcttccc tttccttggc cagcacgttc agccggtcga actgatgatc
      961 gcgctgtacc ttgaccgtct gcggcggcgc caacagcttg ccgtccttgt ttatcagacg
     1021 gaaactcacc tcgtatttca gcaggtattc atcaaccttg ccggtcgagg ttaccgataa
     1081 tacctggctg tccgactgct cgccgtaaag catcaatcgc ggcgcctcac ccaactcctg
     1141 gagctgaaca tccgtctggg aacgcagagc gtttttcatc gctgccagca gcgggttgtt
     1201 ttccatctga ttgccttcga cgctgacctc aagggtcgaa agtggcggtg gcatttcgat
     1261 cttccccgcg ccgcgcaaat gaaaaccaca tacggtcgtt gtgagcgcca acaataaaaa
     1321 aaatgcccgc accataaaat ggcgctcgcg acgaccgtcc ccgcggaagc ggggacccag
     1381 gccaataatg tccttagatt gattcatccc gccacgacga tgttgaccag cttgcccggc
     1441 acgacgataa ccttcttcac cgccttgccg ccaacatgac gcgccacggc ttcgtccgcc
     1501 agcgcgagct cgcgcacttt gtcctcgccc gcctgcaccg gcaccgagac gcgtccgcgc
     1561 agttttccgt tcacctgtac tacgatctcg aggttgtcct gcttcaaggc cgcatcgtcg
     1621 ggcttgggcc agcgcacgtc gattacagcc tctttatggc caagcgcctg ccagagtgcg
     1681 tggcagatat gcggcacgat cggcgacagc gtcaacaccg cgaactcgag cgcttcctga
     1741 cgcaccgcct tggcggacgc cgagttgtca tcgagtctgg cgagactgtt cagcaattcc
     1801 atcaccgacg cgatcgcggt attgaaggtg cggcgccggc cgatgtcgtc ggtgaccttc
     1861 gacagcgttt gatgcgcctg gcggcgcatg tccttcagcg ccggcgacag cgaggagaaa
     1921 tcgcccctcg ttaccagtcc ctgattggca tgttcggcga ccagcttcca caggcggcgc
     1981 agaaaacggc tcgcgccggc cactccctcg tccgaccact cgagcgtcat ttccggcggc
     2041 gccgcgaaca tcatgaacag gcgcgcggtg tcggcgccgt actgctcgat cagtgattgc
     2101 ggatcgacgc cgttgttctt ggacttcgac atggtaccga tgccgcccga ctcgaccggc
     2161 tggccgtcgc tcttgaggac cgtgccgagg cggccgccct tgtcgtcggt tttgatctcg
     2221 acctcggccg gattgaaata ctgaatacgc ccactcgtcg gcttgcgaaa gaaaatctcg
     2281 ttcagcacca tgccctgcgt gagcagccgc atgaacggct catcgccccg tacgaggccg
     2341 atgtcgcgca tgaccttcca ccagaagcgc gagtacagca ggtgcagaat ggcgtgctcg
     2401 atgccgccga tgtactggtc cactggcatc cagtaactgg tttcgccgtc gaccatggcc
     2461 gcgttcgtgc gcttgctggc ataacgcgcg taataccagg acgagtccac gaaggtgtcc
     2521 atggtgtcgg tctcgcgtcg cgctgctttg ccgcacttcg gacactgaca gttcacgaaa
     2581 tcggcgcgct tgttgagcgg gttgccggtg ccatccggca cgcagtcttc cggcagaact
     2641 accggcaaat ccttgtccgg gaccggcaca tcgccgcaac ggtcgcaatg aataataggt
     2701 atcgggcagc cccaatagcg ctggcgcgaa atgccccagt cgcgcaggcg ccacatgacc
     2761 tgcttgtcac cgagaccttt cgctttcagg tcggcggcaa tggcatcgac cgcgg
//
LOCUS       DBNGZE010000258         3128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1768788, whole genome shotgun sequence.
ACCESSION   DBNGZE010000258 DBNGZE010000000
VERSION     DBNGZE010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1768788"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="AC16FV_06675"
     CDS             <1..1419
                     /locus_tag="AC16FV_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR2849776.1"
                     /translation="FTSGCATNPVTGTPDLVFMTEAREIHLGDSYDSKVRSQYGIYPN
                     EALQTYVQGIGQKLAAQSHRPGLKYHFTVLDSPEVNAFALPGGHVYITRGILAYINSE
                     AELAAVLGHEIGHVTARHGVRQHAAATTTGVIGAVLSAATGVQATQDLFNVFGNAMLS
                     GYGRNHELESDRLGAEYLARTGYDPQAMIKVIGILKNQEEFEKKRAEVEKRQPQIYHG
                     VFASHPSTDNRLQEVVAEADKFKTLAAPQVAREVYLKHVDNLGYGDSAGEGVRRGRMF
                     YHRDLNMALTFPEGWRIENSPNAIVSWPPQKDALVMVKKLALPSDSTTSLESHLKSQA
                     KLSSQFIGKPIADMKLPSVTGTDRMSSPFGTRETRVSAVHYGPNAYLFLGTTKEAMAF
                     ARFDSDFIATAASLRPLKAAEKRLAEGQHLRLVRARPGDTFANLAKASPLTEYNEPTL
                     RLINDKYPDGEPQAGELIKIIE"
     gene            1472..2725
                     /locus_tag="AC16FV_06680"
     CDS             1472..2725
                     /locus_tag="AC16FV_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR2849777.1"
                     /translation="MKQDMKQAALDYHAQPTPGKLAVVPTKPCETQQDLSLAYSPGVA
                     EPVREIAKDPEAAYRYTNKGNLVAVISNGTAILGLGNRGSLASKPVLEGKAVLFKRFA
                     NIDVFDIEIDAPDRDSFIDTVANIAPTFGGINLEDIAAPECFAIEEMLRARLDIPVFH
                     DDQHGTAVIICAGLLNALELQGKKLPEARIALLGAGAAGLATMQLLIALGAKRERMLL
                     LDSQGVVHPGRPDLNIYKRRYAQVTDKRTLADAMQGADVFIGVSAANVVLPEMVASMA
                     PKPVVFALANPDPEIRPELAKSVRGDLIMATGRSDYPNQVNNVLGFPYIFRGALDVHA
                     SSVSRSMLVGAVRALAKLAHEPVPAEVLRAYGLERLEFGPEYIIPKPLDSRLIKTIPP
                     AVAKAAVDSGVARAGYPAHYPSPEK"
     gene            2793..>3128
                     /locus_tag="AC16FV_06685"
     CDS             2793..>3128
                     /locus_tag="AC16FV_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR2849778.1"
                     /translation="MNKITIVGAGRVGESAAHNLAKAELCREVVLIDIKEGVPQGTAL
                     DIQESAPIFDFDTTLTGSNDLHAMAGSELVVITAGIPRKPGMSRSDVLDTNLAVVNGL
                     VDAMLKHAPN"
BASE COUNT          657 a         1024 c          908 g          539 t
ORIGIN      
        1 ttcacttccg gttgcgccac caatcctgtc accggaacgc cagacctcgt gttcatgacc
       61 gaggcgaggg agattcatct cggtgacagc tacgacagca aggtccggag ccaatacggg
      121 atttatccca atgaggcgct tcaaacctac gttcagggga ttggccagaa gctggcagcg
      181 caaagccatc gtcccggcct caaatatcac tttaccgtcc tcgacagtcc ggaggtgaat
      241 gccttcgcgt taccaggtgg tcatgtctat atcacgcgcg gcattcttgc ctacatcaat
      301 tccgaggccg aactggcggc ggtgctcgga catgaaatcg gtcacgtcac cgcccgccac
      361 ggcgtgcgcc agcatgcagc ggccaccacc accggcgtga tcggcgccgt gctcagtgcc
      421 gctaccggcg tccaggccac ccaggattta ttcaatgtgt ttggtaacgc catgctctcg
      481 ggttacgggc gcaatcatga gcttgagtcc gaccgtctcg gcgcggagta cctggcgcgc
      541 accggctacg acccgcaggc catgatcaag gttattggca tcctgaaaaa ccaggaagag
      601 ttcgagaaaa aacgggccga ggtcgaaaaa cgccaaccac agatctacca cggcgtattc
      661 gccagtcatc cgagcaccga caatcgtttg caggaagtcg tcgcggaagc cgacaaattc
      721 aaaacacttg cagcgcccca agtcgcgcgc gaggtttatc tgaaacatgt cgacaatctt
      781 ggctatggtg acagtgccgg ggaaggagta cgccggggtc gcatgttcta ccatcgcgac
      841 ctcaatatgg cattgacctt tcccgaaggg tggcgcatcg agaactcccc gaacgccatc
      901 gtcagctggc caccccagaa agacgccctc gtaatggtga agaaactcgc cctgccttcg
      961 gacagcacca cgtcgcttga atcacatttg aagagccagg caaaattgag ctctcaattc
     1021 atcggcaaac cgatagcgga catgaaactg ccatcggtca cgggcacgga ccgcatgtcc
     1081 agcccctttg gtacgcgtga aacgcgcgtt tcggccgtcc actacgggcc gaacgcttat
     1141 ctgttcctcg gcacaaccaa ggaagccatg gcgttcgcac gtttcgattc tgattttatt
     1201 gccacggccg ccagcctgcg tcccctgaaa gcagcggaaa aacgactggc cgaaggtcag
     1261 cacctgcgtt tggtgcgggc gcgcccgggc gatactttcg ccaatctggc caaagcatcc
     1321 cctcttactg aatacaacga accgacactg cgcctgatca acgacaagta tccggacggc
     1381 gaaccgcagg cgggcgaact catcaagatc atcgaatagg taatatccag atggcaagac
     1441 gaatggagcg aagcggagta gggctacatt tatgaaacag gacatgaaac aggcggcgct
     1501 cgactatcac gcgcagccga cacccggcaa gctggccgtg gtgccgacca agccgtgcga
     1561 gacccagcag gacctttcgc tcgcctattc gccgggggtg gccgagccgg tgcgcgagat
     1621 cgccaaggac cccgaggccg cctaccgcta caccaacaag ggcaacctgg tggcggtcat
     1681 cagcaacggc accgccatcc tcggtctcgg caaccgcggc tcgctggcga gcaagccggt
     1741 gctcgagggc aaggcggtgc tgttcaagcg tttcgccaat atcgacgtgt tcgacatcga
     1801 gatcgatgct cccgatcgcg acagtttcat cgacaccgtg gccaatatcg cgccgacctt
     1861 cggcggcatc aacctcgaag acatcgccgc gcccgagtgt ttcgccatcg aggagatgct
     1921 gcgcgcgcgc ctcgacatcc cggtgttcca cgacgaccag cacggcaccg ccgtgatcat
     1981 ctgcgccggc ctgctgaacg cgctcgaact tcagggcaag aaactgcccg aggcgcgcat
     2041 cgcgctgctt ggcgccggcg ccgccgggct ggccacgatg cagctgctga tcgcgctggg
     2101 cgcgaaacgt gagcgcatgc tgctgctcga cagccagggc gtggtccatc ctggccgccc
     2161 ggatctgaac atctacaagc gccgctacgc ccaggttacc gacaagcgca cgctggccga
     2221 cgccatgcag ggggcggacg tgttcatcgg cgtctcggcc gcaaacgtgg tattgcccga
     2281 gatggtggca tcgatggccc ccaaacccgt ggtgttcgcg ctggccaatc ccgacccgga
     2341 aatccgccct gagctcgcca aaagcgtgcg cggcgacctc atcatggcga ccggacgctc
     2401 ggattacccc aaccaagtga acaacgtgct cggcttcccc tacatctttc gcggcgcgct
     2461 cgacgtgcac gccagttccg tctcacgctc catgcttgtg ggcgcggtgc gcgcgctggc
     2521 caaactggcg catgagccgg tacccgccga ggtgctgcgt gcctacggct tggagcgcct
     2581 ggaattcggc ccggagtaca tcatccccaa acccctcgac tcgcgcctga tcaagaccat
     2641 tccgccggcc gtggccaagg cggcggtcga ctccggcgtg gcgcgcgcag gctatcccgc
     2701 gcattacccg agcccggaaa aataaccctc cgcaccgggc aggttttttc gcccggctat
     2761 actggtggaa gaaaaatgta cagggactgg ccatgaataa aatcaccatc gtcggcgccg
     2821 ggcgcgtcgg tgaatccgcc gcgcacaatc tcgccaaggc tgaactctgc cgcgaagtgg
     2881 tgctgatcga tatcaaggaa ggtgtgccgc agggcaccgc gctcgacatc caggaatcgg
     2941 cgccgatctt cgatttcgac accacgctca caggctcgaa cgatctccac gccatggccg
     3001 gctcggagct ggtggtgatc accgccggca tcccgcgcaa gcccggcatg tcgcgttccg
     3061 acgtgctcga caccaacctc gcggtggtca acggcctcgt ggacgcgatg ctcaagcacg
     3121 cgccgaac
//
LOCUS       DBNGZE010000259        12025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_800203, whole genome shotgun sequence.
ACCESSION   DBNGZE010000259 DBNGZE010000000
VERSION     DBNGZE010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12025
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_800203"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..251)
                     /locus_tag="AC16FV_06690"
     CDS             complement(87..251)
                     /locus_tag="AC16FV_06690"
                     /inference="COORDINATES: protein motif:HMM:NF048662.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7210 family protein"
                     /protein_id="HIR2849779.1"
                     /translation="MSSEKTKDAAPKKVKVRLLAEHTHAGEDKKPGDTIDLREDQAKR
                     LFEAKRAEPD"
     gene            complement(290..1042)
                     /locus_tag="AC16FV_06695"
     CDS             complement(290..1042)
                     /locus_tag="AC16FV_06695"
                     /inference="COORDINATES: protein motif:HMM:NF052337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR2849780.1"
                     /translation="MPELFDSKYFRGQGKVFLGDRNATTGAPEGLVFIGDVSAADLSP
                     QVQNEEHIESVTGSGGIGSSFTKSIEYQLTMTMHSIKPDHLAIALQGAATTKAGASVT
                     DEAHVVKLGKFSRMQHNKISAVVVTGAGGTPTYVNNTDYKIHADEGLIEWLTGGTVTE
                     GLAVLIDYAYAAQKWVTANPQNLTKYLVFAGKNSADNSKMTRCEVYKIKLDPGVLGMI
                     QEAHAGMQITGRILLDSLRAAGDQFFGWKIED"
     gene            complement(1059..1505)
                     /locus_tag="AC16FV_06700"
     CDS             complement(1059..1505)
                     /locus_tag="AC16FV_06700"
                     /inference="COORDINATES: protein motif:HMM:NF048220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HIR2849781.1"
                     /translation="MDDYLAAEPLIVARLESQVTGVKVLTAADLAGVKEAAQVTPAVH
                     VLYGGDRLADSVGHGIAQTWAQIWWVVVAVRNARNQGKGREARKAAGPYIAQVFQALT
                     GWQMSSTDHGPLKRVNPPGGPVYSPGGFAYFPLAFETIVQNLGNST"
     gene            complement(1517..1720)
                     /locus_tag="AC16FV_06705"
     CDS             complement(1517..1720)
                     /locus_tag="AC16FV_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2849782.1"
                     /translation="MELGTVKWFNDQKGYGFITPDHGGTDVFVHYSAIPGKGFKTLAE
                     GQRVEFEAMKGPKGMQAKTVQLI"
     gene            complement(1735..2157)
                     /locus_tag="AC16FV_06710"
     CDS             complement(1735..2157)
                     /locus_tag="AC16FV_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HIR2849783.1"
                     /translation="MTYATQQDMIDRFAQTELVELTDRSNTGNIDTTVLNRSLSDADV
                     LIDGYLQGRYTLPLVTVPKVLSLYAGDIARYLLYDNQAPEQVEKRYDDAIKFLQQVAQ
                     GKITLGLSAAGESPAEADGAQMESGGSVFKREDSTGFI"
     gene            complement(2154..3656)
                     /locus_tag="AC16FV_06715"
     CDS             complement(2154..3656)
                     /locus_tag="AC16FV_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849784.1"
                     /translation="MARIPFNAIIGAPKAYGITVVVVALDNIGSMTWSLAGQSGTVTL
                     SKWCDDRPPIPSGPRAGQTWAGWIGSFSISGLSLKGLEQKTGTITQNGVTLPIAFRAQ
                     PSKGQKFTVVKTTCFDKPSIPYSVSADIFNSEDPESPNAFAINNDDLSYVDNRILDDL
                     AGTGHLSTGAPQTTNLLYDFGIAWMYRLGLIGEGMQDEDIWYFSKLNHLFQPGNHEAV
                     TVSSGRTTWDNLFAHCKGDGFDTPRLAVTDCWQRSFGDYKFVCTSSTDIANNTDHVLD
                     VLDELGTVEAFKFNLMARPGPWNAMSAAPVPRFVAGHQRLFYASGQTPPSISDNVKTN
                     GGAGIVLNLNADRHNGWFVKYFAPADTGIVQEKFAIWNCGGTTSAGHIDGSTWELIGG
                     IWVRVDNYDEGTITDNAQVIYKVRLNPNAGIAAYDDPRGPQFAWTRITCHGDEFPPRV
                     TMEHRRQAANEGAVYSAFTRHWTVFKTDNYGSAVPVRMPRAAMSHQMVEA"
     gene            complement(3656..4069)
                     /locus_tag="AC16FV_06720"
     CDS             complement(3656..4069)
                     /locus_tag="AC16FV_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849785.1"
                     /translation="MIRAALFFLLLIAPMTSSARLLSISLGDWDAAYSRVAPKIIVDC
                     DCIVTENPDGTKNVSFNKFRFIKKVGNEISCVIANPSDFLTTIASNPMSLLYALFGGG
                     GASWSSYDNGVTWRRITPGNSVPEVPDAWCLQRLQ"
     gene            complement(4066..4986)
                     /locus_tag="AC16FV_06725"
     CDS             complement(4066..4986)
                     /locus_tag="AC16FV_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849786.1"
                     /translation="MNNFNPLDAGWGSLVEVWDGKVGVVAGTTGTWTGRKAGIVLGGN
                     FTSDGTFCDAAAGVHLTLQSGTLPSPGTKYPAAFVLARLPSGNSAPSLILGDPGGNGP
                     GIQLGLNGAPSYITKDVAHYLESTSGTDAKHPPSDIIAAAIAQTSRTAAVIEWMNKVP
                     NWAINTAYAVGQFRRPTGSSIGSPGLIAEVTAISGTGTSQATDTVAWPATVGATVVDN
                     AGANQITWTMRADVPKSETVSLVGGTDADLTGLTFPQVVQASGDNQTTGWGTIALFYL
                     TNPPTQALVQQALRQMRHFPGKLPLALYGK"
     gene            complement(5007..5501)
                     /locus_tag="AC16FV_06730"
     CDS             complement(5007..5501)
                     /locus_tag="AC16FV_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849787.1"
                     /translation="MLKAILRAISQITGRDEDIKSDAGALLVKIAGSSESGGAVPVRS
                     VYGLPLGSLSGQTPIAASASAVDQTLTQPARGAKFSLGDGSAADPDLYAIITWDMPDT
                     LTRDYVLNESNGGVPRHELRVSDEPLEITFTNPDGTETTISTLHVKFSAATAGAKLHW
                     GIVP"
     gene            complement(5536..5787)
                     /locus_tag="AC16FV_06735"
     CDS             complement(5536..5787)
                     /locus_tag="AC16FV_06735"
                     /inference="COORDINATES: protein motif:HMM:NF036841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HIR2849788.1"
                     /translation="MPTIKVTAKADRFRRAGFSFTREPVELNTDDLSKEQLKALKAES
                     MLVIEEVSSKDKDKDKDKDKDKDKDKNPAGGSAGNTSTK"
     gene            complement(5928..6962)
                     /locus_tag="AC16FV_06740"
     CDS             complement(5928..6962)
                     /locus_tag="AC16FV_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HIR2849789.1"
                     /translation="MKRALFHLFSFAAVMLTAVLMFTAGGPAVPPTLLAHDSALPFIG
                     LAGMIINRANLSALFTGIKTAFNTGFREAATYWEKIATLVPSTTSDEKYGWLGQFPRL
                     REWIGDRYVKSIAAHDYSIKNKTFEATVGIPRENIEDDQFGVYAPLYQEMGHASKTHP
                     DELIFALLAAGFTTTCFDGQFFFDTDHPVGDQEGAAVSVSNMQAGAGNPWFLIDASRP
                     LKPLIFQKRKDYNIQAMTTEDDEKVFMSKEFRYGVDARCNVGFGFWQQAFGSKATLDK
                     TNFDAAMAAGMAFKSDEGRPLGIKFTHLVCGPSNRAAANDVVLTERLANGASNPNFKA
                     VEVMVVPWLT"
     gene            complement(7020..7421)
                     /locus_tag="AC16FV_06745"
     CDS             complement(7020..7421)
                     /locus_tag="AC16FV_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849790.1"
                     /translation="MTALTADRNTPIRDAIDFEYGVGAGSKIFAGAIVMKNATGYAVK
                     GATATGQIALGVAQELADNTSGADGAIKVKVRKGTFRFANDGTITIADIGGTAYIVDD
                     QTVADNNGGATRSALGTIVDVDSTGAWVKIA"
     gene            complement(7462..8514)
                     /locus_tag="AC16FV_06750"
     CDS             complement(7462..8514)
                     /locus_tag="AC16FV_06750"
                     /inference="COORDINATES: protein motif:HMM:NF021627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HIR2849791.1"
                     /translation="MKFKHSPFAIGLAACQFQLQPGSRELQLLPAGHFRSAIDGRPGD
                     VPGGSWFIDAALAQRVITKAQARANPLVIDYEHQTLNAEKNGQPAPASGWWDAAKMVW
                     REGQGLFVTDVDWTDNASAKITAREYKFFSPVFKYDKKTGAVTEILMGAITNFPAIDG
                     MEAIAARAAARFDESNKPDEEIEPMKDLLKLLGLKEDATEAEAITAVKGLQTKITEGE
                     QAIAAAKTQAPDPAKFVSIETHTKLAGDFTALKTDLETRDLNAAIAGAIDEGRLPPGE
                     EQWAREFAKQHGLVALKASLEKRPVIAALKRTQTDGKRPEHDNNGELTDEQIAVCRSM
                     GLSEEEYKKTLGKKAA"
     gene            complement(8727..9164)
                     /locus_tag="AC16FV_06755"
     CDS             complement(8727..9164)
                     /locus_tag="AC16FV_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HIR2849792.1"
                     /translation="MAGARVTLEVDDRQVLSVLDRLIERLGDATPALRDIGEALLNSH
                     RRRFEEQTAPDGTPWAPLSPSYQAKKKKNADKILVLDGSLSGLLTYQVSPDGLELGTN
                     RIYGATHQFGDPARNIPARPFLGLSADDRAKVLEIFSKYLMRG"
     gene            complement(9275..9946)
                     /locus_tag="AC16FV_06760"
                     /pseudo
     CDS             complement(9275..9946)
                     /locus_tag="AC16FV_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
     gene            complement(10119..10346)
                     /locus_tag="AC16FV_06765"
     CDS             complement(10119..10346)
                     /locus_tag="AC16FV_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849793.1"
                     /translation="MRQLKGSIGEVALLEINGKNLRVFRCDRRFPPHKWICTYGDRFT
                     GGDTLIEAITHMRRRGVAVPKTLPKALLSLK"
     gene            complement(10358..11911)
                     /locus_tag="AC16FV_06770"
     CDS             complement(10358..11911)
                     /locus_tag="AC16FV_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HIR2849794.1"
                     /translation="MVAIVDRWGRPIQQETLDESQTSRVAHLAQQFASHPSRGLTPSK
                     LASILEEAERGNLMSQCDLFEDMEEKDAHIFAEMQKRKLALQTVSWDIAPPENASAAE
                     KNAAQAVKEMIQAIPDFEDVLFDLLDAIGKGYSCQEIEWQRLGKSWLPKEVHWRPPSW
                     FQVNQTDQNELRLRDNTAFGAELQAFGWIVHKHRGKSGYLARAGLHRVLAWPYLFKNY
                     SVRDLAEFLEIYGLPVRIGKYPSGASDKEKATLLRAVVSIGHNAAGIIPSGMEMELLE
                     AAKGTHDPFQAMMDWAERSESKAILGQTLSAEAKATGLGSGTADVHDEVRHDILVSDA
                     RQAAATLTRDLVYPIAALNVAGIDPQRAPRLVFDTQEAEDLKLYSEAVPALVDVGLCI
                     PSKWAHEKLRIPEPQNDEEVLARKPGASSFVTLKGMTAGAVAAAAELPIPDDQVDRLD
                     QEAGPVLDAMIGQIRRVVNEATSLEDLRDQLIEIFPQMDAAEFGRVMQRALLAAELAG
                     RFDIMGKLR"
BASE COUNT         2078 a         3756 c         3669 g         2522 t
ORIGIN      
        1 ttttcatcac tcattgccta actcaaaatt aagcgcggcg ggtatacccg ccccgcgatg
       61 acctaaatca ccggcggcgg ttcagttcaa tccggttcgg cgcgcttggc ttcgaacagg
      121 cgcttagcct ggtcttcacg cagatctatg gtgtcgccgg gcttcttgtc ttcgccggcg
      181 tgcgtgtgct ccgccagcag ccgcaccttg accttcttcg gcgcggcgtc cttggttttt
      241 tcgctgctca tgtcgtgtgc tcctgttcag ttagaaagaa tcagccagat cagtcctcga
      301 tcttccagcc gaagaactga tcgccggcgg cgcgcaggct gtcgagcagg atgcggccgg
      361 tgatctgcat gccggcgtgc gcttcctgga tcattccgag cacgcccgga tcgagcttga
      421 tcttgtagac ctcgcagcgc gtcatcttgc tgttgtcggc gctgttcttg ccggcgaaca
      481 ccaggtactt cgtgaggttc tggggattgg ccgtgaccca cttctgcgcc gcgtaggcgt
      541 agtcgatcag caccgcgagg ccttcggtca ccgtgccgcc ggtgagccac tcgatcaggc
      601 cctcgtcggc atgaatctta tagtcggtgt tattgacgta ggtcggcgtg ccgcccgcgc
      661 cagtgacgac caccgcgctg atcttgttgt gctgcatgcg cgagaacttg ccgagcttca
      721 ccacgtgcgc ctcgtcggtg acgctcgcgc cggccttggt cgtggccgcg ccctggagcg
      781 cgatcgccag gtgatccggt ttgatcgagt gcattgtcat cgtcagctga tactcgatcg
      841 acttcgtgaa gctcgatccg atgcctccgg agccggtgac gctttcgatg tgctcctcgt
      901 tctgcacctg cggcgagagg tccgccgcgc tcacgtcgcc gatgaagacc aacccctcgg
      961 gcgcgcccgt ggtggcgttg cggtcgccca ggaagacctt gccctggccg cgaaaatatt
     1021 tactgtcgaa tagttcaggc attgatgtgc tccttacgtt aagttgaatt gcccaggttc
     1081 tgaacgatgg tctcgaacgc cagggggaaa taggcgaagc cgcccggcga gtacaccggc
     1141 ccgcccggcg ggttcacgcg cttgagcggt ccgtgatcgg tgctgctcat ctgccagccc
     1201 gtgagtgcct gaaacacctg cgcgatatac ggcccggccg ccttgcgcgc ctcccggccc
     1261 ttgccctggt tgcgcgcgtt acgcaccgcg acgaccaccc accagatctg cgcccaagtc
     1321 tgggcgatac cgtggcccac gctgtccgcc aggcggtcgc cgccataaag cacgtggacc
     1381 gccggcgtga cctgcgcggc ttccttgacg ccggcgagat ccgccgcggt caacaccttg
     1441 acccccgtca cctggctctc caggcgggcg acaatcagcg gttcggcggc gaggtaatcg
     1501 tccattcaaa cgtcctttaa atcagttgta cggttttggc ctgcatgccc ttcggcccct
     1561 tcatcgcctc gaactccacg cgctggcctt cggcgagggt cttgaagccc ttgcccggga
     1621 tcgcggagta atgcacgaac acatccgtgc cgccgtgatc cggggtgatg aagccgtagc
     1681 ctttctgatc gttgaaccac ttcaccgtgc caagttccat gtctgttttg cctctcaaat
     1741 aaatccagtc gagtcttcgc gcttaaaaac actgccgccc gattccatct gcgcgccgtc
     1801 ggcttcggcc ggcgattcgc cggcggcgct caatcccaag gtgatcttgc cctgcgccac
     1861 ctgctgcagg aacttgatcg catcgtcgta acgcttctcc acctgctcgg gagcctgatt
     1921 gtcatacagc aggtagcgtg cgatgtcgcc ggcatagagc gacaacacct tcggcaccgt
     1981 tacgagcggg agcgtgtagc ggccctgcag ataaccgtcg atcaatacat cggcatcgct
     2041 cagcgagcga ttgagcaccg tcgtgtcgat gttgccggtg ttgctgcgat cggtgagttc
     2101 gaccagctcc gtctgcgcga agcggtcgat catgtcctgt tgggtggcgt aggtcatgct
     2161 tccaccatct ggtggctcat cgcggcgcgc ggcattctca ccggaaccgc cgagccgtaa
     2221 ttatcggtct tgaaaaccgt ccaatgccgc gtgaaggcgc tgtataccgc gccttcgttc
     2281 gccgcctgcc gccgatgctc catcgtcacg cgcggtggaa attcgtcgcc gtggcaggtg
     2341 atccgggtcc aggcgaattg cggaccgcgc ggatcatcgt aagcggcaat tcccgcattc
     2401 gggttcagcc ggaccttgta gatcacctgc gcgttgtcgg tgatggtgcc ttcgtcataa
     2461 ttgtccacgc gcacccagat accgccgatc aattcccacg tcgagccgtc tatgtgtccg
     2521 gcgctggtcg taccgccgca attccagatg gcgaatttct cctgcactat gccggtgtcg
     2581 gccggcgcga aatacttcac gaaccagccg ttgtgccgat cagcgttgag attcagaacg
     2641 attcccgcgc caccgttggt cttgacgtta tcgctgatcg agggcggcgt ctgccccgac
     2701 gcgtagaaca ggcgctggtg accagccaca aaacgcggca ccggagcggc gctcatggcg
     2761 ttccacggtc ccggtcgcgc catcaaattg aacttgaatg cctcgaccgt tcccaattca
     2821 tcgagaacat ctagcacgtg gtcggtattg ttagcgatat cggtgctgct ggtgcaaacg
     2881 aacttgtaat cgccaaagct gcgctgccag caatcggtga ccgcgaggcg cggtgtgtcg
     2941 aaaccgtcac ccttacagtg ggcaaacagg ttatcccacg tcgtgcgccc ggaactgact
     3001 gtaacagcct catgattccc cggttgaaac aggtgattca gtttgctgaa ataccagatg
     3061 tcctcgtcct gcatgccctc gccgatcagc cccagccgat acatccacgc gatgccgaag
     3121 tcataaagca gattcgtcgt ctgcggcgct cccgtcgaaa ggtgcccagt gccggcgaga
     3181 tcgtcgagga tgcggttatc cacgtaactc agatcgtcat tgttgatggc aaaagcgttc
     3241 ggcgactccg ggtcctcgct gttaaatata tcggcgctga ccgaataggg gatggacggt
     3301 ttatcgaagc aagttgtttt gacaacggtg aatttctgtc ccttggatgg ctgcgcccga
     3361 aaggcgatag gtaacgtcac gccattctgc gtgatcgtcc cggttttctg ctccagtcct
     3421 ttgaggctca ggccagagat gctgaaactg ccgatccacc cggcccaggt ctgccccgcg
     3481 cgcgggccgg agggaattgg cgggcggtcg tcgcaccact tgctgagtgt gaccgtgccg
     3541 gattgtccgg cgagactcca tgtcatcgac ccaatgttgt ccagcgcgac gactacaacg
     3601 gtgattccat aagccttcgg cgcaccgatg atcgcgttaa aagggatgcg cgccattatt
     3661 gcaacctctg caagcaccac gcatcaggca cttctggaac actgttcccc ggcgtaatcc
     3721 gccgccacgt cacgccgttg tcatagctcg accagctcgc gccgccaccg ccgaacaggg
     3781 catacagcaa cgacatggga ttactcgcta tcgtcgtcag aaaatccgag gggttggcga
     3841 tgacgcagga tatttcatta cctacttttt tgataaagcg gaatttattg aagctgacgt
     3901 tttttgtgcc atcgggattc tcggtcacga tgcaatcgca atccacgatg atcttgggcg
     3961 cgacgcggct ataggcggca tcccagtcgc cgagactgat gctgagcaac cgagccgatg
     4021 aagtcatcgg cgcgatgagc agcagaaaaa acaatgccgc ccgaatcatt tgccgtacaa
     4081 cgccagcggc agcttgccgg ggaagtgccg catctggcgc aacgcctgct gaacaagcgc
     4141 ctgcgtcggc ggattggtta aatagaacag cgcgatggtg ccccagccag tcgtttgatt
     4201 atcgccactg gcctgaacga cctgcggaaa tgtcagcccg gtaagatcgg cgtcggtgcc
     4261 acccaccagc gataccgttt cagatttcgg aacatccgcg cgcatcgtcc aggtgatctg
     4321 attcgcgccc gcgttgtcca ccaccgtcgc cccgactgtc gccggccacg cgacggtatc
     4381 ggtggcctgc gaggtgccgg tgccggaaat cgccgtgact tccgcgatca aacccggact
     4441 tccgatcgat gaacctgttg gacggcggaa ctggcctacc gcgtaggcgg tattgatcgc
     4501 ccaattaggc accttattca tccactcgat gacggcagcg gtgcgcgagg tctgcgcgat
     4561 ggcggcggcg atgatgtccg agggcggatg tttcgcgtcg gtgccagatg tgctttcgag
     4621 ataatgcgca acatctttgg tgatatacga cggcgctcca ttcaacccga gctgaatgcc
     4681 ggggccgttt ccaccggggt ctcccaatat caatgacggt gcagagttgc cgctcggcaa
     4741 ccgagctaaa acaaacgcag caggatattt ggtgccgggt gatggcaagg ttcccgattg
     4801 caacgtcaag tggacaccgg cagcggcatc acagaaggtt ccatccgatg tgaaattccc
     4861 tcccagcaca atgccggctt tccggcccgt ccacgtcccg gtggttcccg caacgacacc
     4921 caccttcccg tcccagacct cgaccagaga accccaaccc gcatcgagcg gattgaagtt
     4981 gttcatgacg ctccagggta tgtctctcat ggcacgatcc cccagtgcag tttggctccg
     5041 gcggtcgcgg cgctgaattt gacgtgcagc gtgctgatgg tggtttctgt cccgtcaggg
     5101 ttggtgaaag tgatttccag cggttcatcg cttacgcgaa gttcgtggcg cggcacgccg
     5161 ccgttggatt cgttcagcac gtagtcgcgc gtgagagtgt ccggcatgtc ccacgtaatg
     5221 atcgcgtata aatcaggatc ggcggcggag ccgtcgccca gggagaattt agctccgcgc
     5281 gccggctggg ttagggtttg atcaacggcc gaggcggatg cggcgatggg cgtttgccct
     5341 gagagcgaac ccagcggcag gccgtaaacc gaacgcaccg gcaccgcgcc gccgctctcg
     5401 ctcgaaccgg cgattttcac gagcaacgcg ccggcgtcgc tcttaatatc ctcatcgcgc
     5461 ccggtgatct ggctgatggc ccgcaggatc gccttgagca tgttttaatc ctcgatgtcg
     5521 gtgatcgcgg aggagttact tcgtgctggt gttgcccgcc gatccgccag cagggttttt
     5581 gtccttatcc ttatccttat ccttatcctt atccttatcc ttgtccttgg acgaaacctc
     5641 ttcgatcacc agcatggact cggccttgag cgccttgagc tgctccttgc tgagatcgtc
     5701 ggtgttgagt tcgaccggct cgcgggtgaa agaaaagccg gcgcggcgga agcggtcggc
     5761 tttggcggtg actttgatgg tcggcatgtt cgttctcctg ttcgtttcgg ttccggccct
     5821 ctggccaaac atggggagga tgtccacgcc ctccccatgc cttgggttgc ctccggcgat
     5881 cccctacgtg gtcagggaaa cgccctcatc ggccgcgtcg tgggttatta ggtcagccag
     5941 ggcaccacca tgacttccac ggctttgaag ttcgggttgc tcgcgccgtt ggccaggcgt
     6001 tcagtgagca ccacgtcgtt cgccgctgcg cggttcgagg ggccgcaaac caggtgcgtg
     6061 aacttgatgc cgagcggccg gccctcgtca gatttgaagg ccatgccagc cgccatcgcg
     6121 gcgtcgaagt tggtcttgtc gagtgtcgcc ttgctgccga aggcctgctg ccagaaaccg
     6181 aaaccgacgt tgcagcgggc atccacgccg tagcggaatt ccttgctcat gaacaccttc
     6241 tcgtcatcct cggtggtcat ggcctggatg ttgtagtcct tgcgcttctg gaagatgagc
     6301 ggcttgagcg gccgactcgc atcgatcagg aaccaaggat tgcccgcgcc ggcctgcatg
     6361 ttcgagacgc tgacggcagc gccttcctgg tcgcccaccg gatggtcggt atcgaagaag
     6421 aactggccat caaaacaggt ggtagtgaag ccggcggcga gcagcgcaaa gatcaactcg
     6481 tccggatgtg tcttggaggc gtggcccatc tcctgataga gcggggcgta gacgccgaat
     6541 tgatcgtcct cgatgttctc gcgcgggata ccgaccgtgg cctcgaaagt tttgttcttg
     6601 atcgagtagt cgtgcgccgc gatgctcttc acgtagcgat cgccgatcca ttcgcgcagc
     6661 cgcgggaact gaccgagcca gccgtatttc tcgtcgctcg tggtgctcgg caccagggtg
     6721 gcgatctttt cccagtacgt ggccgcctcg cggaagccgg tgttgaacgc cgttttaatg
     6781 ccggtaaaaa gggcgctcaa attggcccgg ttgatgatca tgccggcgag tccgatgaag
     6841 ggcagggccg agtcatgggc cagaagcgtg ggcggcacgg caggaccgcc ggccgtgaac
     6901 atcagcaccg ccgtcagcat gacggcggcg aaactgaaga gatggaacaa agcgcgtttc
     6961 atgatggtgt gtctcctgta ggttgcgccg agggttttcg ataagtgtgc gatgcagcgt
     7021 taggcgatct tgacccaggc gccggtgctg tcgacgtcaa cgatggtgcc gagcgccgag
     7081 cgcgtcgcgc cgccgttgtt gtccgccacg gtctggtcgt cgacgatgta ggccgtgccg
     7141 ccgatgtcgg cgatggtgat cgtgccgtcg ttggcgaagc ggaaggtgcc tttgcggacc
     7201 ttgaccttga tcgcgccgtc ggcgccgctg gtgttgtcgg ccagctcctg ggccacgccc
     7261 agggcgatct ggccggtggc ggtcgcgccc ttgacggcgt aaccggtggc gttcttcatc
     7321 acgatcgcgc cggcaaagat ttttgaacct gcgccgaccc catactcgaa gtcaatggcg
     7381 tcccggatcg gggtgttgcg gtcagccgtc agtgcagtca tcgttttctc ctgtcaggtt
     7441 tcggttggaa ggccgcgggg cttaggccgc tttcttgccc agcgtcttct tgtattcctc
     7501 ttcgctcagg cccatcgagc ggcacaccgc aatctgttca tcggtgagtt caccgttgtt
     7561 atcgtgctcg ggacgcttgc cgtcggtctg cgtgcgcttg agcgcggcga tgaccgggcg
     7621 cttttccagg ctcgccttga gcgcgaccag cccgtgctgc ttggcgaact cgcgtgccca
     7681 ctgttcctcg cccggcggca gccggccttc gtcgatcgcg ccggcgatcg cggcgttgag
     7741 atcgcgcgtc tcgagatcgg tcttgagggc ggtgaagtca cccgcgagct tcgtgtgcgt
     7801 ctcgatcgag acgaacttcg ccggatcggg cgcctgcgtc ttggccgcgg cgatcgcctg
     7861 ctcgccttcg gtgatcttcg tttgcagacc cttgacggcg gtgatggcct cggcttcggt
     7921 cgcgtcttcc ttcaggccca acagcttgag caagtctttc atcggttcaa tctcctcgtc
     7981 aggtttattg gattcatcga aacgcgcggc ggcacgggcg gcgatcgcct ccatgccgtc
     8041 gatcgcggga aaattggtga tcgcgcccat cagaatttcg gtcaccgcgc cggtcttttt
     8101 gtcgtacttg aagaccggcg agaaaaactt gtactcacgg gcggtgatct tggcgctggc
     8161 gttatcggtc cagtcgacgt cggtgacgaa cagcccctgg ccctcacgcc acaccatctt
     8221 cgccgcatcc caccacccgg acgccggcgc gggctggccg ttcttttcgg cattgagcgt
     8281 ctgatgctcg tagtcgatga caagaggatt ggcgcgtgcc tgcgcttttg taattacgcg
     8341 ttgcgcgagc gcagcatcga tgaaccaact gccgccgggg acatcgcccg gacgcccatc
     8401 gatcgccgag cgaaaatggc ccgcaggcag aagctgcagc tcgcgcgatc cgggctgcaa
     8461 ctggaactga cacgccgcca ggccgatcgc gaacggggaa tgcttgaatt tcaaccgcgc
     8521 acctcgatga atgaacgagg cgcagtatcg ggaagatcgg gcgggggaat ctattaaagc
     8581 gtttgaaaaa atgatttgaa ccggagagcg cggcgcgcag gatggagcgc gacagttcat
     8641 ttgtcaaacg cacgcagcga gacacgcgcc tgagcgcgag ggttgattag ggcagaaaag
     8701 ggtagatctg ggtaaaagat cgcgcgtcag ccgcgcatga ggtacttgct gaaaatctcc
     8761 agcaccttgg cgcggtcatc cgcggagaga ccgaggaaag gacgcgcggg aatgttgcgc
     8821 gcgggatcgc cgaactgatg cgtcgcgccg tagattcgat tagtgcccag ctccaggccg
     8881 tcgggcgata cctggtaagt cagcagcccg ctcagggaac catcgagcac caggattttg
     8941 tcggcgttct ttttcttctt cgcctggtag ctcggcgaga gcggcgccca gggcgtgccg
     9001 tccggcgcgg tctgctcttc gaagcgtcgg cgatgagaat tgagcaacgc ctcgccgatg
     9061 tcgcgcagcg ccggtgtcgc gtcgcccagg cgctcgatga gccgatccag gaccgacagc
     9121 acctggcgat catcgacttc gagcgtgact ctcgcgccgg ccattacacg tttctccgat
     9181 tttcaatctc ggcgaggaag gcttcgcgaa ttcccgccgg catctgctcg ctcttgcgcg
     9241 ccagctcgtc gcgcagctgc gcgctacggt cgaacgagcc gggctggtac tcgaagcccg
     9301 gatcgatgcc ttgcggtacg accaccgtgc gcgggttcgg ccccgtgatg ccgacagttt
     9361 tttcttccca ctgaatcggt ggcgcttcgt caggcccgtc tttgtcgagc ttgcgcaggt
     9421 cgcgctccgc gagcgtctcg acgtaacact gacagcccca accgttcggc gggaagtgcg
     9481 tgcgccacca cggatcgtcg tggcgcaaca ccaggccatc ccaggcgaga tgctccgggc
     9541 gcggatgctc gacggcatcg ctgtggcggt agcgccagta cggacggctc tcggcgactt
     9601 cctgcatctg cgcgtatcgc cccgcggcgt agctcgtgcg caggttggtg tcgtagatca
     9661 cgcgcgtgcg ccagttgcgc ccgcccttgt agctccagcc atgctcctcg acgatcgcat
     9721 cgaagtcccg gcgaaattcc gcgagcgtcg tcccctcgct gatcgccttg tcgatcgcgt
     9781 tgtagaaatc gagaacgaga tccgccttgg tcgcgcccgc caccatgaac gcgatgtcgt
     9841 gctcggcggc gtagatgtcg gtccaggcgc gcgtcggcag ccggagcttg cggcggaaga
     9901 agagaatctg ctcctcgaag ggcagggaac cgtagcgggc gggcatcagc cacccccaaa
     9961 gtgggggtat agaccgcccc gggagtggcc cgggaaggca tttaaatacc ctttaatttt
    10021 ttcgatcttt tttgaaacgg cccgtttccg cgtaaaaaac cgatccgcgc ccctagaggc
    10081 tctcaggcgg tttaacgcga tagaggcgca actcatggtt atttcaggct taaaagggct
    10141 tttggcagtg ttttcggcac cgccacccca cgccggcgca tgtgggtgat ggcctctatt
    10201 aaggtgtcgc cgccggtaaa gcggtcgccg taggtgcaga tccacttatg cggcgggaac
    10261 cggcgatcgc aacgaaaaac acgaaggttt ttgccgttga tctcaagcag ggcaacctcc
    10321 ccgattgacc ccttgagctg gcgcacgagc cacctgctca tcgcagcttg cccatgatgt
    10381 cgaaccggcc cgccagctcg gccgcgagca gcgcccgctg catcacccgc ccgaactcgg
    10441 cggcgtccat ctgcgggaag atctcgatca gctggtcgcg cagatcctcc aggctcgtgg
    10501 cctcgttcac gacacgccgg atttgcccga tcatggcgtc gagcaccggg ccggcttcct
    10561 ggtcgagccg gtccacctgg tcgtcgggga tcggcagctc ggctgcggcg gccacggcgc
    10621 cggcggtcat ccccttcagc gtcacgaacg acgaagcgcc gggcttgcgc gcgagtacct
    10681 cttcgtcatt ttgcggttcg ggaatgcgca gcttctcatg cgcccacttg cttgggatgc
    10741 acaggcccac gtccaccagc gccggcaccg cttccgagta gagtttgagg tcttccgctt
    10801 cctgcgtgtc gaacaccagg cgcggcgcgc gctgtggatc gatgccggcg acattgagcg
    10861 cggcgatcgg atagacaaga tcgcgcgtga gtgtggccgc cgcctggcgc gcgtccgaaa
    10921 cgagaatgtc gtgacgcact tcgtcgtgca cgtcggccgt gccgctgccc aagcccgtgg
    10981 ccttcgcctc ggccgaaagc gtctgcccga ggatcgcctt cgattcgctg cgctcggccc
    11041 aatccatcat cgcctggaac ggatcgtgcg tgcccttcgc ggcttcgagc agctccatct
    11101 ccatgccgct cgggatgatg cccgcggcgt tgtggccgat gctgaccacc gcgcgcagca
    11161 gcgtcgcctt ttccttgtcg ctcgcgccgc tcggatactt tccaatgcgc accggcagtc
    11221 cgtagatctc cagaaattcc gcgagatcgc gcaccgaata atttttgaac agatacggcc
    11281 acgcgagcac gcgatgcaac ccggcgcgcg cgaggtaacc gctcttgccg cgatgcttgt
    11341 gcacgatcca gccgaaggcc tgcagctcgg cgccgaacgc ggtgttgtcg cgcagccgca
    11401 attcgttctg atcagtctgg ttgacctgaa accaggacgg cggacgccag tgcacttcct
    11461 tcggcaacca gctcttgccc aggcgctgcc actcgatctc ctggcacgag taacccttgc
    11521 cgatggcgtc gagcagatcg aacaacacgt cctcgaaatc gggaatcgcc tggatcatct
    11581 ccttgacagc ctgcgcggcg ttcttctccg cggcgctggc gttctccggc ggcgcgatgt
    11641 cccaggagac ggtttgcagc gcgagcttgc gcttctgcat ctcggcgaag atgtgcgcgt
    11701 ccttttcctc catgtcctcg aacagatcgc actgcgacat caggttgccg cgctcggcct
    11761 cttcgaggat cgaggcgagc ttcgacggcg tgaggccgcg gctcgggtgc gaggcgaact
    11821 gctgcgcgag atgtgcgacg cgcgaggtct gcgactcgtc cagcgtctcc tgttgaattg
    11881 gtcggcccca acgatccacg attgctacca tgcaccacct ccagcattac tcagataatg
    11941 atcgtcctcg tcgtcatcgg gcttcccgtc ccaggcgcgc gccttgtccg gcgccggcgt
    12001 ccagtcgatc tcgtaaccct ccatc
//
LOCUS       DBNGZE010000260         1997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1633788, whole genome shotgun sequence.
ACCESSION   DBNGZE010000260 DBNGZE010000000
VERSION     DBNGZE010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1633788"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="AC16FV_06775"
     CDS             <1..536
                     /locus_tag="AC16FV_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR2849795.1"
                     /translation="LPWGQEVPLRPFFGVMAVAPPAAWGVIPTPPPRRNGGNIDNKEL
                     VAGTTLYLPIFTDGALFSVGDGHDAQGDGEVCITAIETGLIGTFRLTVRDDIRLEWPM
                     AETPTHVMTMAFDPDLDDCVVIALRQMLDLVCARAGLDRYQAYTLLSLAADLRITQVV
                     NGNKGVHVMLEKQYLMR"
     gene            complement(591..>1997)
                     /locus_tag="AC16FV_06780"
     CDS             complement(591..>1997)
                     /locus_tag="AC16FV_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR2849796.1"
                     /translation="PWSNGKVGLSGISYYAINQWQVASLQPKHLAAICVWEGFADFYR
                     ELSHNGGIYNTFAQNWYDMQVKTVQYGLGTKGHRSRMNGDWVSGPETLTAEQMGANRY
                     DLGKAYFAHPLDDDFYKAMRPDWSKVKVPMLSAANWGGQPLHPRGNFEGFYRAASKQK
                     WLEVHGIEHWTHYYTDYGVGLQKKFFGYFLKGERNGWDKHPRVQLNIRHPGENFVIRH
                     EDDWPLPRTQWTKFHLATDAQKLVTTAPSGSAEVTFDAIGDGVTFLTEPVKEETEITG
                     PVAAKLHVSSSTSDADLFLVLRVFSADMKEVVFQGALDPHTAIGQGWLRASHRKLDKN
                     LTRPFRPYHTHDKMQPLKAGEVYELDIEIIPTCIVVPKGYRIALSIRGRDYVYPGGSG
                     GRLSNMKNEFTGNGPFLHDDPRDRPLEIYGGKTTLHLGAGRENFVLLPVIPPRKAALA
                     KAKASKPAKRAARKKKRR"
BASE COUNT          366 a          588 c          618 g          425 t
ORIGIN      
        1 agctaccgtg gggacaagag gttccgctgc ggccgttctt cggcgtgatg gcggtggcac
       61 cgccggcggc ctggggcgtg atcccgacgc cgcctccgcg ccgcaacggc ggcaatatcg
      121 acaacaagga gctggttgcc ggcacgacac tttacctgcc gatcttcaca gatggcgcac
      181 tgttttccgt gggcgacggt cacgacgcgc aaggcgacgg tgaagtctgc atcaccgcca
      241 tcgagaccgg tctcatcggc acgtttcggc tgacggtgcg tgacgacata aggctggaat
      301 ggccgatggc ggagacaccc acacacgtca tgacgatggc cttcgaccca gacctcgacg
      361 attgcgtcgt tatcgcgctg cgacaaatgc tcgacctcgt gtgtgcgcga gcgggccttg
      421 accgctacca ggcctacacg ctgttgagtc ttgccgccga cttgcgcatt acgcaggtgg
      481 tcaacgggaa caagggtgta cacgtgatgc tggagaagca gtacctgatg cgttgacccg
      541 cagccgttcg tggattccgc ttgcgcggga atgagtggag tatcgggccg ctaccgccgt
      601 ttcttcttcc tcgccgcccg cttcgctggc ttggacgcct ttgcctttgc tagcgcggcc
      661 ttgcgcggcg gaatgaccgg caacaagacg aaattctccc gaccggcgcc gagatgcaat
      721 gtcgttttgc cgccgtaaat ttccagcggc cgatcgcgcg ggtcatcgtg caggaagggc
      781 ccgttgccgg tgaattcgtt tttcatgttg gagagccggc cgcccgagcc gccgggatag
      841 acataatcgc ggccgcggat actcagtgcg atgcgatagc ccttcggcac cacgatgcag
      901 gtcgggataa tctcgatgtc gagctcgtaa acctctccgg ccttgagcgg ctgcattttg
      961 tcgtgggtgt gatagggacg gaatggccgc gtaagattct tgtccagctt gcggtgcgat
     1021 gcgcgcaacc agccttgccc gatcgccgtg tgcgggtcga gtgcgccctg aaacaccact
     1081 tctttcatgt cggccgagaa cacgcgcagg actaggaaaa ggtccgcatc gctggtcgat
     1141 gatgagacgt gaagcttggc ggccacgggc ccggtgattt ccgtttcttc cttcacgggc
     1201 tcggtaagga acgtgacgcc gtcgcctatc gcatcgaagg tgacctctgc cgagccgctg
     1261 ggagccgtcg tgaccagttt ctgcgcatcc gtggccagat ggaattttgt ccactgcgta
     1321 cgcgggagcg gccagtcatc ttcgtggcgg atgacgaaat tttcgcccgg gtggcggatg
     1381 ttaagctgta cgcgagggtg cttgtcccaa ccgttgcgct caccttttaa aaaatacccg
     1441 aagaatttct tctgcagacc aactccgtag tcggtgtaat aatgtgtcca atgctcgatg
     1501 ccgtgcactt cgagccattt ctgcttggac gctgcgcgat agaagccctc gaaattgccg
     1561 cggggatgca gcggctgccc gccccagttt gcggccgaaa gcatgggtac cttcaccttc
     1621 gaccagtcag gccgcatcgc cttgtagaag tcatcgtcga gcggatgcgc gaaataggcc
     1681 ttgccgagat cataccggtt ggcgcccatt tgttcggcgg taagcgtctc ggggcccgac
     1741 acccagtcgc cgttcatacg gctgcggtga cctttggtgc caagaccgta ctgcacggtc
     1801 ttgacctgca tgtcatacca attctgcgcg aaggtgttgt agatgccacc gttgtggcta
     1861 agctcgcgat agaaatcggc aaacccttcc cagacgcaga tggcggcgag atgtttgggt
     1921 tgcaggctcg cgacctgcca ctggttgatg gcgtaatagg aaatcccgct aagacccact
     1981 ttaccgttcg accacgg
//
LOCUS       DBNGZE010000261         6764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_452505, whole genome shotgun sequence.
ACCESSION   DBNGZE010000261 DBNGZE010000000
VERSION     DBNGZE010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_452505"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1594)
                     /locus_tag="AC16FV_06785"
     CDS             complement(<1..1594)
                     /locus_tag="AC16FV_06785"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2849797.1"
                     /translation="MNDLAKELGSGIPAANATPDYARLAPLSRPVATSQHGTPLDLDK
                     LHPKLAGVPLRINGEETHSTNSTRYKQHGFHFNADNCIACHACESACSEKNNLPPHLA
                     FRKVGYLEGGSWPDVRRLNISMACNHCENPVCLKACPTRAYTKYAEYGAVLQDPDICF
                     GCGYCTWVCPYNAPQLDPVNGQVEKCNMCVDRLEQGLKPACVAACLGNALEFGVIEDL
                     PQGREQVKLAIPGFPDPSISRPNIRFQQVRSLPPSLRRTDGVPLQYQRGAPGAGRFEV
                     KTNLTSGKRTWGFDKLSSRENPLVAFTLLSQFTVGAFLALFLSPLVAGPAAAAFGAHP
                     LLASVAGWLLVALQAVALSLSASHLGKPHRFYRGFNNLRHSWLSREALVLSLFFGALA
                     AYMLATSFPALTSWLPSAFYAALPMLMGGGAALLGPAAIYCMYRIYRIKARPFWDHWH
                     TGAAFFASLMILGSLGTGLAFGMAEWLAGRAPDGLLRWLSLPLLAGLAMQALALALHW
                     RYLSRSSGEADVSRAQMLTTYGK"
     gene            complement(1594..3774)
                     /locus_tag="AC16FV_06790"
     CDS             complement(1594..3774)
                     /locus_tag="AC16FV_06790"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006031795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR2849798.1"
                     /translation="MLFGRRHKNSIKIPVKPVEKWAYTTCGYCSTGCSIEVGLDKNGQ
                     AITARGVGNAPVNRGKLCLKGIFEFELFNSSNRGKVPRLRENFFDPYRETTWEAALDR
                     TAAEIQRIQETHGRDAFAIVSTGQILTEEFYTLGKLARGVIGTNNYDGNTTLCMASAV
                     SGYKRSFGSDGPPGCYDDFNHTECLLAIGSNLPEQHPIIYWRLKQARDARKFPLIVVD
                     PRVTVFAQMADMHLPVNPGTDVVLLNSLAHVILKENLHSRDYIAAHTNGFDEFAKLVE
                     QYDPTTAAGICGIDEDTIRNVARLYGGARAAMTIWTMGINQSTHGSDGVVAINNLNLI
                     TGNIGKPGGTSLSITGQCNAMGTREFSSCSGLPAYRYLENKKDREFMAEYWGVDEDFF
                     PAKRGLFQTDIFPAIETGQIKGLWLIATNPMSSMPNTPRIRKTLEKLEFLVVQDAYAD
                     VETTQYAHAYLPAALWGEKEGVFTNTERRVNLVRKVMNPPGEARSDLWIFNELAKRFP
                     KPAKLGFPETPAGVFEEMRELSTGRLCDYSGMTHDRIEHHRGIQWPCNDRHPEGSARI
                     YTDGVFQHADGKAKLIPLPFVDNNERPDEQYPFWLNTGRVVEHWHTRTKTGKIGNLNK
                     FSPTPYIEINPSAAEKLGVGHMEYVRVVSRRSDAIIMAQLTERVAPDAVFIPMHFHEC
                     VNRLVLGLLDPYSRQPAFKQSAVRIEKIADQATAAEINLTARSF"
     gene            4032..4502
                     /locus_tag="AC16FV_06795"
     CDS             4032..4502
                     /locus_tag="AC16FV_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR2849799.1"
                     /translation="MSLTLASSGFQHQGEIPARYTCDGDDNSPPLAWSGVPAGAKSLV
                     LIVDDPDAPDPKAPKMTWVHWVLYNIPPTVTSLPEHVAPKNLPKGTLEGINDWKRTGY
                     GGPCPPIGRHRYFHKLYALDILLPDMNRPTKAKVESAMRGHILSQAEIVGTYER"
     gene            4566..5462
                     /locus_tag="AC16FV_06800"
     CDS             4566..5462
                     /locus_tag="AC16FV_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR2849800.1"
                     /translation="MRHPPTEKITSGTHRLFPRHFRLLLACLYLAGISACGTLPNQPD
                     AAAPPDEKTIADAGATEKDNAHDPLEGFNRAMYTFNDKFDKYLLKPVAKGYRAITPKP
                     VSKSIANFFGNLHDPGIMLNNLLQGKPKQAVSDLGRFLVNSTIGIAGLFDVATKMGME
                     KHNEDFGQTLAKWGVGDGPYLVLPFFGPSNVRDGAGLPVDWEMYPPNHMEETSTRDKL
                     LVVETVDKRAQYLEAGDILEQAAGQDPYIFVREAYRQRRINQIHDGNPPQAAPPPGLF
                     EEDEPAPKAPSGPAPGGATQSR"
     gene            complement(5450..>6764)
                     /gene="aceB"
                     /locus_tag="AC16FV_06805"
     CDS             complement(5450..>6764)
                     /gene="aceB"
                     /locus_tag="AC16FV_06805"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR2849801.1"
                     /translation="VERKMIINALNSGANVFLADLEDSLAPTWDNVVRGQINLHDAVR
                     RTISYDSPEGKHYALNPRTAILFVRPRGWHLIEKHMLVDGQPVSASLFDFGLYFFHNA
                     RALLERGTAPYFYLPKLESYLEARLWNEVFVWAQERLKIPRGTIKATVLIETILAAFE
                     MDEILYELREHSAGLNCGRWDYLFSSIKKFSHDPNFILADRGLVTMTAPFMRAYSLLL
                     IHTCHRRNAHAMGGMAAQIPIKNDPAANEAALTKVRTDKEREVHDGHDGTWVAHPGLV
                     ALAKEVFDQNMKMPNQIHRKREDVRVTASDLLAFSPSAPITQAGLRQDIEVGIEYLGA
                     WLAGTGCVQIHHLMEDAATVEISCSQTWHWIHSKKGVLSDGRKVTLELARKLIPEELQ
                     RIRQSLGEAAYAAGKYELAAKLFDKFISTEPLPQFLTLLGYDYLD"
BASE COUNT         1282 a         2014 c         2170 g         1298 t
ORIGIN      
        1 ttttgccata agtcgtcagc atctgcgcgc gcgacacgtc cgcttcgccg ctgctgcggg
       61 agagataacg ccagtgcaac gcgagcgcca gcgcctgcat ggcaaggccc gcgagcaacg
      121 gcagcgagag ccagcgcagc aggccatccg gcgcgcggcc ggcgagccat tccgccatcc
      181 caaaggcaag gcccgtgcct aaacttccca gaatcatcag gctcgcgaaa aacgccgcgc
      241 ccgtgtgcca atggtcccag aagggacgcg ccttgatgcg atagatacgg tacatgcagt
      301 agatcgcggc gggtccgagg agcgcggcgc ccccgcccat gagcatcggc agggccgcgt
      361 aaaacgcgga aggcaaccag gacgtcagtg ccggaaacga ggtggcgagc atgtaggccg
      421 ccagcgcccc gaagaagagc gacagcacca gcgcctcgcg cgagagccac gagtggcgca
      481 gattgttaaa accgcggtag aagcggtgcg gtttgccgag atgagaggcc gagagcgaga
      541 gcgccaccgc ttgcagcgcg accagcagcc atcccgcgac ggaggccagc aaggggtgcg
      601 cgccgaatgc cgccgcggcc ggaccggcga ccaagggtga caggaacagc gcgaggaacg
      661 cgccgaccgt gaattgcgac aggagcgtga aggcgaccag aggattttcg cgcgagctca
      721 gtttgtcgaa accccacgtg cgctttcccg atgtcagatt ggttttgact tcgaaacgcc
      781 ccgcgcccgg agcgccgcgc tgatactgga gcggcacgcc gtcggtgcgc cgcaggctcg
      841 gcggcagcga gcgtacctgt tggaagcgga tattggggcg cgagatcgac ggatccggga
      901 atccggggat ggcgagcttc acctgttcgc gtccctgggg caggtcttcg atgacgccga
      961 attcgagcgc gttgccgaga caggccgcga cgcaggccgg cttcaggccc tgttccagcc
     1021 ggtccacgca catgttgcat ttctcgacct ggccgttgac cggatcgagc tgcggcgcgt
     1081 tgtacgggca cacccaggtg cagtagccgc agccgaaaca gatatcgggg tcctgcaaca
     1141 ccgcgccgta ctcggcgtac ttggtgtagg cgcgcgtggg gcaggccttg aggcacaccg
     1201 ggttctcgca gtggttgcag gccatggaga tattgaggcg gcgcacgtcc ggccaggagc
     1261 cgccctcgag ataaccgacc ttgcggaagg cgaggtgcgg cggcaggttg ttcttctccg
     1321 agcacgccga ctcgcaggca tggcaggcga tgcaattgtc ggcgttgaaa tgaaagccgt
     1381 gctgcttgta gcgcgtggaa ttggttgaat gcgtctcctc gccgttgatg cgcagcggca
     1441 ctccggcaag cttcggatgg agcttgtcga gatcgagcgg cgtgccgtgc tggctagtgg
     1501 cgacgggccg tgacagcgga gcgagccggg cataatccgg cgtcgcattg gctgccggta
     1561 tcccgctgcc gagttccttc gccagatcgt tcatcagaag ctcctcgccg tgaggttgat
     1621 ttccgcggcg gtggcctggt ccgcgatttt ctcgatgcgc accgccgact gcttgaacgc
     1681 gggctggcgc gaataggggt cgagcaggcc gagcaccagg cggttgacgc actcgtggaa
     1741 gtgcatgggg atgaacacgg cgtcgggcgc cacgcgctcg gtcagttgcg ccatgatgat
     1801 ggcgtccgag cggcgcgaga cgacgcgcac gtattccatg tggccgaccc cgagcttttc
     1861 cgccgcggaa gggttgatct cgatatacgg cgtcgggctg aatttgttga gattgccgat
     1921 cttgccggtc ttggtgcgcg tgtgccagtg ttcgaccacg cgcccggtgt tgagccagaa
     1981 cggatactgt tcgtccggtc gctcgttgtt gtccacgaac ggcagcggga tgagctttgc
     2041 ctttccatcg gcgtgctgaa acaccccgtc ggtataaatc cgcgcgctgc cttccggatg
     2101 cctgtcattg cacggccact ggatgccgcg atgatgctct atccggtcat gcgtcatgcc
     2161 ggagtagtcg cacagccttc ccgtcgacag ttcgcgcatt tcctcgaaca ccccggcggg
     2221 ggtttcggga aaaccgagtt ttgccggttt tggaaagcgc ttggccagct cgttgaaaat
     2281 ccagagatcg gacctggcct cgcccggcgg gttcatcacc ttacgcacga ggttcacgcg
     2341 ccgctcggtg ttggtgaaca cgccttcctt ctcgccccac agcgccgcgg gcaggtacgc
     2401 gtgggcgtac tgcgttgtct cgacgtcggc ataggcgtcc tgcaccacga ggaattcgag
     2461 cttttccagc gtcttgcgga tgcgcggggt attcggcatc gaactcatgg gattggtggc
     2521 gatgagccac aggcccttga tctgcccggt ctcgatggcc gggaagatgt cggtctggaa
     2581 caacccgcgc ttggccggga aaaagtcctc atccacgccc cagtactcgg ccatgaactc
     2641 gcgatccttt ttgttttcca gatagcgata agccggcaga ccagaacagg aggaaaattc
     2701 gcgcgtgccc atggcgttgc actggccggt gatggacagc gaggtgccgc ccggcttgcc
     2761 gatgttgccg gtgatgagat tgagattgtt gatcgcgacc acgccgtcag agccgtgcgt
     2821 ggactgattg atgcccatgg tccagatggt catggcggcg cgcgcgcctc cgtacaggcg
     2881 cgcgacgttg cggatcgtgt cttcgtcgat gccgcagata ccggcggccg tcgtcgggtc
     2941 gtattgttcg acgagcttgg caaactcgtc gaagccgttc gtatgcgccg cgatgtaatc
     3001 cctggagtgc aggttttcct tgaggatcac gtgcgccagg ctgttcagca gcacgacgtc
     3061 ggtgccggga ttgacgggca ggtgcatgtc cgccatctgc gcgaagaccg tgacgcgcgg
     3121 gtccacgacg atgagcggaa acttgcgcgc atcgcgtgcc tgcttgagcc gccagtagat
     3181 gatcgggtgc tgctccggca ggttcgatcc gatggcgagc aggcattcgg tgtgattgaa
     3241 atcgtcgtag cagccgggcg ggccgtcgct gccgaaggag cgcttgtagc ccgacaccgc
     3301 cgaggccatg cacagcgtgg tgttgccgtc gtaattgttc gtgccgatca cgccgcgcgc
     3361 gagcttgccg agcgtgtaga attcctccgt cagaatctgg ccggtggaga cgatggcgaa
     3421 ggcgtcgcgg ccatgggttt cctggatgcg ctggatctcc gcggcggttc tgtcgagcgc
     3481 cgcctcccac gtggtttccc gataaggatc gaaaaagttt tcgcgcaggc gcggcacctt
     3541 gccgcggttg gaggaattga acagctcgaa ctcgaagatg cccttgagac acagtttgcc
     3601 gcggttgacg ggggcattgc ccacgccgcg cgcggtgatg gcctggccgt ttttatccag
     3661 gccgacctcg atcgagcagc ccgtggaaca atagccgcag gtggtgtagg cccatttttc
     3721 cacgggcttc accggaatct tgatcgaatt tttgtgacgg cgtccgaaaa gcatgaggtt
     3781 tccaagtgat ggtttgccat cctcaatagc aatgcctgtg ccagtctgga acggattatg
     3841 gttcaatgag ttagcctgca accgcttgca acaaagcacc aaaacgttcg gtatcgcgag
     3901 ggccgtgcgc ctacttggtg caacggcaac ggaggtatga tgacgctgct cacggcgccc
     3961 atttttccgc tggcgggagg cgcgcgatga atggccggtg cgccatccgg caatgtccag
     4021 gagaaccatg catgtcgctg actcttgctt ccagcgggtt ccaacatcag ggtgaaatcc
     4081 cggcgcgtta cacctgcgac ggcgacgata attcgccgcc gctcgcgtgg tccggcgtgc
     4141 cggccggcgc caagagtctt gttctgatcg tggacgaccc agacgcgccc gatcccaagg
     4201 cgccgaaaat gacatgggtg cattgggtgc tctataacat tcccccgacg gttaccagtt
     4261 tgccggaaca cgttgcgcca aaaaatcttc ccaagggaac actggagggt atcaacgact
     4321 ggaagcgcac gggttatggc ggaccttgtc cgcccatcgg tcgccaccgg tatttccata
     4381 aactttacgc gctcgatata ttgttgccgg acatgaaccg cccgaccaag gccaaggtcg
     4441 agagcgccat gcgcgggcat atcctttccc aagccgagat cgttgggacc tatgagcgtt
     4501 agcgccgagt cagatcaaat ggcgcggact ttgcagaaac agccgaattc cattagccta
     4561 gcggcgtgcg ccatcccccc actgaaaaaa taacaagcgg cacccatcgc ctgtttcccc
     4621 gccatttccg tctgctgctg gcctgtctgt atcttgccgg aatcagcgcc tgcggcacgc
     4681 tgccgaatca gcccgatgcc gcggcgccgc cggatgagaa gacgatcgcc gatgccggtg
     4741 caacggaaaa ggataacgcg cacgacccgc tggaagggtt caaccgtgcc atgtatacct
     4801 tcaatgacaa atttgacaaa tatctgctca agcctgtcgc caagggttat cgcgcgatta
     4861 cgcccaagcc ggtgagcaag agcatcgcaa atttcttcgg caatctgcac gacccgggga
     4921 tcatgctgaa caacctgcta cagggaaaac ccaagcaggc cgtttcggac ctcggtcgct
     4981 tcctggtgaa ttccaccatc ggtatcgccg gcctgttcga tgtcgcgacc aagatgggta
     5041 tggaaaaaca taacgaagat ttcggccaga ccctggcaaa gtggggcgtg ggtgacgggc
     5101 cttatctggt gttgccattc ttcgggccaa gtaacgtgcg cgacggcgcc gggttgcccg
     5161 tggactggga gatgtatccg ccgaatcaca tggaagagac aagcacgcgc gacaagctgc
     5221 tggtggtgga aacggtggac aaacgcgcgc aatatctcga agccggcgat attctcgaac
     5281 aggcggccgg ccaggacccg tatattttcg tgcgcgaagc ttatcgtcag cgccggatca
     5341 accagattca cgacggcaac ccgccgcagg ccgcaccacc accgggcctg ttcgaggagg
     5401 acgaaccggc gcctaaggcg ccatccggcc ctgcgccggg tggggcaact cagtcgagat
     5461 agtcgtagcc caacagcgtc aggaactgcg gcaggggttc ggtagagata aatttatcga
     5521 acaattttgc ggcaagttca tatttaccgg cagcataagc agcttcaccg agcgattgac
     5581 ggatgcgctg gagttcctcc gggatcagct tccgcgccag ttccagcgtc accttgcgcc
     5641 cgtcgctgag cacgcctttc ttgctgtgta tccagtgcca ggtctgcgag caggagattt
     5701 ccacggtggc ggcgtcttcc atgaggtgat ggatctgcac gcagccggtc cccgccagcc
     5761 acgcacccag gtattcgata ccgacctcga tatcctgccg cagcccggcc tgcgtgatcg
     5821 gcgcagaggg ggagaaggcg agcagatcgg aggcagtcac acgcacgtcc tcgcgcttgc
     5881 gatgaatctg gttcggcatt ttcatgttct ggtcaaaaac ctcttttgcc agcgccacca
     5941 gtcccgggtg cgccacccag gtgccgtcgt gcccgtcatg gacctcgcgt tccttgtccg
     6001 tccgtacctt ggtcagcgcg gcctcgttcg ccgctggatc gttcttgatc gggatctgcg
     6061 ccgccatgcc gcccatggca tgggcgttgc gccggtggca ggtatggatc agcagcagcg
     6121 agtacgcgcg catgaacggt gcggtcatgg tgaccagccc gcggtcggcg aggatgaaat
     6181 tggggtcgtg gctgaatttc ttgatggagc tgaaaagata atcccagcgc ccgcaattga
     6241 gaccggccga atgctcacgc aactcgtaca ggatttcatc catctcgaac gcggccagta
     6301 tggtttcgat cagtaccgtg gctttgatgg tcccgcgcgg aattttcagg cgctcctgcg
     6361 cccacacgaa cacctcgttc cataacctgg cctcgagata gctttccagc ttgggcagat
     6421 agaaataggg cgcggtgccg cgttccagca gcgccctggc gttgtgaaag aaatacaggc
     6481 cgaaatcgaa caggctggct gagaccggct ggccgtccac cagcatatgc ttttcgatca
     6541 gatgccagcc gcgcggacgc acgaacagga tcgccgtgcg cgggttcagc gcgtaatgct
     6601 tgccttccgg gctgtcgtaa ctgatggtgc gcctgaccgc gtcgtgcagg ttgatctgcc
     6661 cgcgcacgac gttatcccag gtgggcgcga gcgagtcttc gaggtcggcg agaaagacat
     6721 tggccccgga gttgagcgcg ttgatgatca tcttgcgctc cacc
//
LOCUS       DBNGZE010000262         1535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1564364, whole genome shotgun sequence.
ACCESSION   DBNGZE010000262 DBNGZE010000000
VERSION     DBNGZE010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1564364"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC16FV_06810"
     CDS             complement(<1..295)
                     /locus_tag="AC16FV_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2849802.1"
                     /translation="MKLNILYHHRTQGRGAEGVHITSIVHALQAMGHSVTVLSPPGVD
                     PLDPKYNTPVDKSKVKTGGVQTLWKWISRHMPNFLFELAEIGYNLPAWWRLRRM"
     gene            complement(310..1404)
                     /locus_tag="AC16FV_06815"
     CDS             complement(310..1404)
                     /locus_tag="AC16FV_06815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2849803.1"
                     /translation="MRVLHVIETLDFGGAEKVAVSLANGMVESHEVAICCVKRVGVLG
                     AKLDKRVRIFCLDKGEGNDYLLPLRLARLIRQHAFDVVHTHNWAVFLEGGLAGLLART
                     PVLLHTVHGPYTDYPLTLGSRLKIALRHLIERRLARRFRRIVAVSDAIRGYIEEDIRI
                     PADHLVTVHNGIPAATSGACAQRPERPHITFMTVGRLAPVKNHMMMLQAFREIAQTHP
                     QARLMIVGDGPELPALENYVRQQRLEDKITFPGFRDDIDALLPEADVFLLTSRYEGIS
                     IALLEAMRAGLPAIGTAVGGIPETIIDGKTGLLVAPNGLEILVKAMTKLAASKSLRDT
                     MGKQGYDYFMQEFTLASMLARYQQLYTNAV"
BASE COUNT          294 a          433 c          474 g          334 t
ORIGIN      
        1 acatgcgtcg taaccgccac caagcgggaa ggttatagcc gatttcggca agttcaaaaa
       61 gaaaattcgg catatgccgg ctaatccatt tccacagcgt ctggacgccg ccggttttga
      121 ccttggactt gtccacgggc gtgttgtatt tggggtcgag cgggtccacg cccgggggcg
      181 agagcacggt gaccgagtga cccatggcct gcaaggcgtg cacgatgctg gtgatgtgca
      241 cgccctcggc gccgcgaccc tgcgtgcggt ggtgatacag gatattgagt ttcatggtgg
      301 ctggaagctt cagacggcgt ttgtgtacag ttgttgatac cgcgccagca tggaggcgag
      361 cgtgaattcc tgcatgaaat aatcgtagcc ctgtttgccc attgtatcgc gcagcgattt
      421 cgaggcagcc agctttgtca ttgccttgac caggatttcc agaccgtttg gggcgaccag
      481 caggccggtt ttaccgtcaa tgatggtctc cggtatcccg cccaccgctg tgccgatggc
      541 cggcagaccg gcgcgcatgg cctccagcag ggcgatcgag atgccctcat agcgtgaggt
      601 cagcaggaaa acatccgctt ccggcagcag ggcgtcgatg tcgtcacgaa atcccggaaa
      661 ggtgatcttg tcttccagcc gctgttgtcg gacgtagttt tcgagggccg gaagttccgg
      721 accatcccct acgatcatca ggcgagcctg cggatgagtt tgtgcgattt cgcgaaatgc
      781 ctgcaacatc atcatatgat tcttgaccgg ggccaggcgt ccgaccgtca tgaaggtgat
      841 gtgcggtcgt tccggtcgct gcgcgcaagc accgctggtg gcggcgggta tgccattgtg
      901 caccgtgacc aggtggtcgg ccggaatgcg aatgtcctct tcgatatagc cgcggatcgc
      961 atccgagacg gcgacgatgc ggcggaaacg tctcgcgagc cggcgctcga tcagatggcg
     1021 cagcgcgatc ttgaggcgcg aacccagggt cagcgggtaa tccgtatagg gaccgtgcac
     1081 ggtatgcagc aacactggcg ttctcgccag gagtccggcc aggccgcctt ccaggaacac
     1141 cgcccagttg tgggtatgca cgacatcgaa agcgtgttgt ctgatcaggc gcgccagacg
     1201 cagcggtaat aaataatcgt tgccttcgcc tttatcgaga cagaaaatcc ggacgcgttt
     1261 gtcgagtttg gcgccaagga cgcccacgcg tttcacgcag cagatggcga cctcatgcga
     1321 ttccaccatg ccgttggcca ggctgacggc aaccttttcg gcgcccccga aatccagcgt
     1381 ttcaattacg tgcaagacac gcaagcgtca ttctccgcgt gattccactg gctgtttcga
     1441 gccatgggcg agttctttga ttttttcgta catttcccag gccgtgacgt agtgcagggc
     1501 gcattcgggc ccgcggcggt attgtttctc caatt
//
LOCUS       DBNGZE010000263         4147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_592620, whole genome shotgun sequence.
ACCESSION   DBNGZE010000263 DBNGZE010000000
VERSION     DBNGZE010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_592620"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="AC16FV_06820"
     CDS             complement(<1..591)
                     /locus_tag="AC16FV_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR2849804.1"
                     /translation="MEKAVMAAFEHDRSINLHRYPIRLEFDIARGALTLEGEVENIIA
                     KKRAFEIASQEDRVQGVIDRLTVAPSERRGDGAIRDSLVQELLEEPALLHCTIRVQHK
                     GAMETLRESRDASGNVINVFVKEGIIQLNGAVPSLSHKRLAGVLAWWTPGCRDVINGM
                     RVMPPETDNDDEIADALRLVLEKEPLIHADQIGVRVK"
     gene            complement(663..1352)
                     /locus_tag="AC16FV_06825"
     CDS             complement(663..1352)
                     /locus_tag="AC16FV_06825"
                     /inference="COORDINATES: protein motif:HMM:NF018946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HIR2849805.1"
                     /translation="MAKKAKPAAKDMASINPDQIISTDERQRMVAEAAYFHALERGFT
                     GDPLDDWLIAEREINHLLPTPQQQKEELAAYDKLRQAVRKILAEARGTVNAETVRQAF
                     DRATDELKKTGEYTAETVSKIATSLRKDMASASLKMGPKWETFTEKSADLFEVWRDRG
                     SLFLAQAASAVGEWLQQAGAKLEQQLYHTGEMVHSGTFECTGCGERVVLNTPAHLPPC
                     SKCRKMEFRRV"
     gene            complement(1388..1921)
                     /locus_tag="AC16FV_06830"
     CDS             complement(1388..1921)
                     /locus_tag="AC16FV_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2849806.1"
                     /translation="METATETRKTEGKKEVTATEKGQEIQKAQPAGALSPFEEMDRMF
                     DQYFRHGWMRPWRFEWPSFPEISLPEMKVPKVDVVDRDNEIVVRAAVPGVEKKDLDVS
                     VSDNAVTIKGSTRHEEKEEKGDYYRHEISTGAFSRTVALPADVDGSKAKASFKDGMLE
                     LTLPKVEKSKRHSIELD"
     gene            complement(2197..2319)
                     /locus_tag="AC16FV_06835"
     CDS             complement(2197..2319)
                     /locus_tag="AC16FV_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849807.1"
                     /translation="MTIDLYKARIEILRLHGKGLPLFGARGRGDLDLRIEVMIP"
     gene            2530..>4147
                     /locus_tag="AC16FV_06840"
     CDS             2530..>4147
                     /locus_tag="AC16FV_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2849808.1"
                     /translation="MATIERLDPKALCARCDPARFTFKTTSELDDLTEVVGQARAMQA
                     IEFGIGICRDGYNLFVLGTPGTGRHSVVRQFIEHKAATEPVPPDWCYVNNFEQQHKPR
                     LLELSAGTGAKLRRDMEQLIEDLRSAIPAAFESEEYRARRQELEQELKERQEHAFEEL
                     RQQAEGHGISMIRTPSGMAFAPVRKGEVLSPEEFHKLPEAEQKRIEAVVTSLEEQLEQ
                     IIHRLPQMRREGQRRIRELERDVTMSAVGHLIDELKKEYATLPKVVSYLEAVQQAVID
                     NADDFRRQEEGQEINILGISLSRAAGASALRRFQVNVLIDHSGAKGAPVVYEDNPSYQ
                     SLVGRVEHISQMGALMTDFTLIKPGALHRANGGYLILDVRKILMQPYAWEGLKRALSS
                     QEVRIESLGQALSLVSTVSLEPEPIPLNIKVVLLGERLLYYLLNYFDPDFGNLFKVAA
                     DFEDTMIRTEESDLLYARLIATIARKEGLLALDRAACARLIEHGARMAADAEKLSIRL
                     RDITDHLREADYWARQAGRDVVTGDDVQRAL"
BASE COUNT          812 a         1228 c         1154 g          953 t
ORIGIN      
        1 cttcacacgg acaccgatct gatcggcatg gatcaggggt tctttttcca gcaccaggcg
       61 cagcgcgtcg gcaatttcat cgtcgttatc ggtctcggga ggcatcaccc gcatgccgtt
      121 gatgacatct cggcaaccgg gcgtccacca tgccagcacg ccggcgaggc gcttgtgcga
      181 caggctcgga acagcaccat tcagctgtat gatgccttct ttcacaaata cattgatcac
      241 attgccagac gcatccctgg attcacgcag ggtttccatc gcgcctttgt gctgcacgcg
      301 gatggtgcag tgcaatagcg ccggttcctc caacaactcc tgaacgagcg agtcgcggat
      361 agcgccgtca ccccgccgtt cgctgggcgc cacggtgaga cgatcgatca caccttgtac
      421 tctgtcctcc tggctggcga tctcgaacgc gcgcttcttg gcaatgatgt tctcgacctc
      481 gccttcgagg gtgagcgctc ccctggcaat gtcgaattcg agccggatag gatagcggtg
      541 cagattgata ctgcggtcgt gttcaaacgc cgccattacc gctttttcca cggacgtcat
      601 ctccggcatc gtcgtcctcg ctatcctgac accacagcag ccgcctgaac ccgtctgcag
      661 aactagacgc gtcggaactc catcttgcgg cacttcgaac acggtggcag gtgggccggg
      721 gtgttcaata ccacgcgttc gccgcacccc gtgcattcaa aggtaccact gtggaccatc
      781 tcgccggtgt ggtagagctg ttgttcgagc ttggcacccg cctgttgcag ccactcgccg
      841 accgcgctgg cggcctgcgc cagaaacagg ctgccgcggt cgcgccagac ctcaaacaaa
      901 tcggccgatt tctccgtaaa cgtctcccac ttcggaccca ttttcagcga cgcgctggcc
      961 atgtccttgc gcagcgaggt ggcgattttg ctgacggtct ccgcggtata ttccccggtc
     1021 tttttgagct cgtcggtcgc cctgtcgaag gcctgtctca cagtctcggc attcaccgtc
     1081 ccgcgcgctt cggcaagaat tttcctgacc gcctggcgca gtttatcgta agccgccagt
     1141 tcctctttct gctgctgcgg agtcggaagg agatggttta tttctctttc ggctatgagc
     1201 caatcatcca gcgggtcacc ggtaaaacca cgctccaatg cgtgaaaata tgcggcttcc
     1261 gcgaccattc tttgacgctc gtcggtggag atgatctgat cgggattgat gctggccata
     1321 tccttcgcgg ctggctttgc ttttttcgcc atggtgttcc tcctgtttat ccatgcactg
     1381 acaacgctca atccagttcg atgctgtgcc gcttggattt ctccaccttg ggcagtgtca
     1441 gctccagcat accgtccttg aagctagcct tggccttgga gccgtccacg tcggcaggca
     1501 acgccaccgt gcggctgaag gcgccggtcg aaatttcgtg acggtagtaa tcgccctttt
     1561 cttccttctc ctcatgccgc gtggaaccct tgatcgtcac cgcgttgtcg ctgacggaga
     1621 cgtccagatc cttcttctcc acgccgggca cggcggcgcg cacgacgatc tcgttgtcgc
     1681 ggtcgaccac gtccactttc ggcaccttca tctccggcag cgagatctcc gggaagctcg
     1741 gccactcgaa gcgccaaggg cgcatccagc catgccggaa gtactggtcg aacatgcggt
     1801 ccatctcctc gaacgggctg agcgcccccg ccggctgagc cttctgaatt tcctgccctt
     1861 tttccgtcgc ggtcacttcc tttttaccct cggtctttct ggtttctgtt gcggtttcca
     1921 tgatctcgcc tccttgcagt atcggttaga taaaaccacc aagaaatata aatttatatt
     1981 gttacaaaat aaggtaatga atggtggaac acattgacat tcctcaaagt tagcgccgat
     2041 agcatcacct gaaagagcca tggattttta aaggcttatc gcctctgcga tcctcgttgt
     2101 ttttattgtt gtttttattc acgtcgtccc ccaccggtgc gaagggccca tcccgcgcag
     2161 cttttcgtac agggcacgct cttcggccgt gagtcgttac ggaatcatga cctcgatgcg
     2221 caagtcgaga tcgccgcggc cgcgcgcgcc gaacagcggc agccccttgc cgtgcaggcg
     2281 caggatctca atccgagctt tgtacaggtc gatagtcaca tccagtcata cgcgcaactt
     2341 catccccgtc cggagcctga acagccgact tcgataggtt ccttggatat tccgtatctg
     2401 cctgtttcca aacaggggga cggaaattga tttcgctcaa tattaagctg ccatggtcgg
     2461 cgctaacctt tgtgcatgtt gtctcgtggt ggtgacaaca acgattttta tgggcaaaaa
     2521 ggagagaaca tggccaccat cgaacgcctg gaccccaaag cgctttgtgc gcgctgtgat
     2581 ccagcccggt ttaccttcaa gaccacttca gagctggatg acctgacgga ggtcgtgggc
     2641 caggcccgcg ccatgcaggc tatcgagttc ggcatcggta tttgccgcga cggttacaat
     2701 cttttcgttc tgggcacccc cggaaccggg cgacacagtg tggttcgtca attcatcgaa
     2761 cacaaggccg cgacagagcc tgtgccaccg gactggtgtt acgtcaataa tttcgaacag
     2821 cagcataagc cgcgtctgtt ggagttgtcc gctggcacag gcgcaaagct gcgccgtgac
     2881 atggaacagt tgatcgagga tttacgcagc gccattcccg cggctttcga aagcgaggaa
     2941 taccgcgcgc ggcgtcagga acttgagcag gaactcaagg aacggcagga gcacgccttc
     3001 gaagaacttc ggcagcaagc tgaaggccat ggaatttcca tgatccgcac gccctccggc
     3061 atggcctttg cgcctgtacg caagggtgag gtgctaagtc ctgaggagtt ccataagctg
     3121 cccgaggccg aacagaaacg cattgaggcg gtggtgacca gcctggaaga gcaactggag
     3181 cagatcattc accggctgcc gcagatgcgt cgcgaggggc aacggcgtat tcgtgaactc
     3241 gagcgtgatg tcacgatgtc tgctgtcgga catctgatcg atgaactgaa aaaagaatat
     3301 gccacgttgc cgaaagtcgt gagttacctc gaggcggtgc aacaggcggt gatcgataac
     3361 gccgatgatt tccggcgcca ggaagaaggc caggaaatca acattctggg tatttccctt
     3421 tcacgcgcag caggcgcctc ggcactgcgg cgatttcagg ttaacgtgtt gatcgatcac
     3481 agcggtgcca agggtgcgcc ggtcgtatat gaggacaacc ccagttatca gtcgctggtc
     3541 ggaagggtcg agcatatctc ccagatgggc gcgctcatga cggacttcac actgatcaag
     3601 cctggtgcgt tgcaccgggc gaatggcggt tatctgattc tcgatgttcg caagattctg
     3661 atgcagccct atgcgtggga gggcctgaag cgcgcgctat cttcgcaaga agtgcgcatc
     3721 gaatctctcg gccaggcctt aagccttgtc agtacggttt cacttgagcc cgaacccatt
     3781 ccactcaata tcaaggtcgt actgctgggt gaacgtctgc tttattacct gctcaactat
     3841 ttcgaccccg atttcggcaa tctcttcaaa gtggccgcgg atttcgagga cacaatgata
     3901 cgcacggagg agagcgattt gctctacgcc cgccttatcg ccaccatcgc gcgcaaggag
     3961 ggtttgctcg cgctcgatcg ggcggcgtgc gcgcgcctga tcgagcatgg tgcacgtatg
     4021 gccgcggacg ccgagaagct ttctatccgt ctgcgtgaca tcaccgatca tttgcgcgaa
     4081 gcggactact gggcgcggca ggctggacgc gatgtggtga ccggggatga tgtacagcgt
     4141 gcgctcg
//
LOCUS       DBNGZE010000264         4564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_939219, whole genome shotgun sequence.
ACCESSION   DBNGZE010000264 DBNGZE010000000
VERSION     DBNGZE010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_939219"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="AC16FV_06845"
     CDS             <1..1560
                     /locus_tag="AC16FV_06845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR2849809.1"
                     /translation="FVRHPEHERFLEEDWFFEGMTETYLPLLNVFEGLARDGVHFRVT
                     MSLTPTLLSMMTDPLLQQRYLRYLDERLEALEGERHRTEGNAYFNPVILFYSDLYTRL
                     RRRFVEQDRMDVTQAFRRLMEAGHLDIITCGATHGYFPLLAVNEESLYAQLSMSVRTH
                     QRILGRRPRGIWLPECGYKPGVDRLLREFGIEYFILDSHGLLNAEPRPKYGVHRPIRT
                     PEGPAAFARDYESSKQVWAAEEGYPGDSTYREFYRDAGFDVDEPHIRKLRHAGIATFT
                     GLKYHRITGKTDWKEPYHPGWARERAAEHAGHFMFNRQQQVRWLASFFDRPPIILAPY
                     DAELFGHWWFEGPQWIDFLLRKIAHDQHDIKTITPSEYLDRFPDLQPVAPPECSWGAG
                     GFHEVWLNGANDWIYPYLHSATERMSALVRRFPNADGLLRWALDQAARELLLAQSSDW
                     PFIMKTGTSVDYAVRRFKTHLHRFHRLTDMIQSGNYDEGYLREVTARDSLFPDMDYRI
                     FQTRTWLSSSG"
     gene            complement(1580..2320)
                     /locus_tag="AC16FV_06850"
     CDS             complement(1580..2320)
                     /locus_tag="AC16FV_06850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2849810.1"
                     /translation="MISAKLKKLVHYFRQGGVYEVLLRLTASEYFPGWLLYFNEAQIL
                     RLDGLDSHAARHALSDYVYEQADRRAIDELLACSGATTQATRRKHFGEFFDRRARCHV
                     MKHDGGIVAYCWTFEDKYVLTYDEYRRKNIVFALDNNEVFMGNVFVAQEHRHHGVFSW
                     LFHQTLSRYPENTRFYSWAERTNDVSLQAHAHLGFAPLFRVLCVTICGITGYWMHAAT
                     ENGWRHISREELERSGLDPPDGRDNHQD"
     gene            complement(2440..3435)
                     /locus_tag="AC16FV_06855"
     CDS             complement(2440..3435)
                     /locus_tag="AC16FV_06855"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR2849811.1"
                     /translation="MRVLAGDIGGTKTLLQIAECKAGRCRAVRKQRFDSRSYDSLSPI
                     IREFLKQEREKNIKAVCFGVAGPVRETAKGQSVKVTNLPWEIHSQELKRRFKLPRLLL
                     VNDFQAIGYGIEALGKKDLTVLQKGKAIRHGPRALIGAGTGLGQGLLFWNRDHYMPVA
                     TEGGHANFAPANELQIELAHYLLKNAGRTSWELVLSGHGLVELYGFLKARGNMPESPS
                     VAGMMRNGDPAAAITQAALEQNDALANETLNLFVDIYGAQAGNLALTAGATGGVYIAG
                     GIAPKIISRLTDGRFMRAFLNKGKMKPYVADLPVQVVTRPEVGLIGAVLAATRLE"
     gene            3515..3601
                     /locus_tag="AC16FV_06860"
     tRNA            3515..3601
                     /locus_tag="AC16FV_06860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3549..3551,aa:Leu,seq:caa)
     gene            complement(3938..4414)
                     /locus_tag="AC16FV_06865"
     CDS             complement(3938..4414)
                     /locus_tag="AC16FV_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849812.1"
                     /translation="MAQSGPTDYSQLVSTIGQLVTWALVICGWLVVNWQHNKREARKE
                     LRAQLDKLRTTLSELEGQAEQYHTAPEHSEAEARRVKVMLQRLVYSLDHLNLLNNLAR
                     EMKVIALRRAITYRNFDTPKHSAQAIGGELIAGINAAVDDLVNALEERFRSHFPVT"
     gene            complement(4392..>4564)
                     /locus_tag="AC16FV_06870"
     CDS             complement(4392..>4564)
                     /locus_tag="AC16FV_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HIR2849813.1"
                     /translation="GVRGYGSSFLEEAFGGLVRAGYSPDLVRKTFKFVSTDSSIIAEI
                     EDYIVHGAERAD"
BASE COUNT          962 a         1386 c         1222 g          994 t
ORIGIN      
        1 ttcgtgcgcc atccggagca tgagcgcttc ctcgaggaag actggttctt cgagggcatg
       61 accgaaacct acctgcctct gctcaatgtc ttcgaaggcc tggcgcgcga cggcgtgcac
      121 tttcgcgtga cgatgtcgct cacgccgacg ctgctctcga tgatgaccga tccgttgttg
      181 cagcagcgct acctgcgtta tctcgacgaa cgccttgagg cgcttgaagg cgaacgccat
      241 cgcaccgaag gcaacgccta tttcaacccg gtgatattgt tctacagcga cctgtatacg
      301 cgcctgcgcc gtcgcttcgt ggaacaggat cgcatggacg tgacgcaggc gtttcgccgc
      361 ctgatggagg ccggccatct cgacatcatc acctgcggcg cgacccatgg ttattttccg
      421 ctgctcgcgg tcaacgaaga aagcctgtat gcccagcttt ccatgtcggt gcgcacgcat
      481 cagcgcatcc tcggccggcg gccgcgcggc atctggctgc cggagtgcgg atacaagccg
      541 ggtgtggatc gcctgttgcg cgaattcggc attgaatatt tcatcctcga ctcgcacggc
      601 ctgctcaacg ccgaaccgcg tccgaagtac ggcgtgcacc gcccgatccg cacacccgaa
      661 ggcccggccg ctttcgcgcg cgactatgaa tccagcaaac aggtatgggc cgcggaagaa
      721 ggttatccgg gcgattccac ctatcgcgag ttttaccggg acgccggatt cgacgtggac
      781 gaaccgcaca tacggaaatt gcgtcacgcc ggcattgcca cgttcacggg cctcaagtat
      841 caccgcatca cggggaagac cgactggaag gagccctacc accccggctg ggcacgcgag
      901 cgcgcggcgg aacatgcggg gcatttcatg ttcaaccgcc agcaacaggt cagatggctg
      961 gcctccttct tcgaccgtcc gcccattatt ctcgcgcctt acgacgccga gttgttcggt
     1021 cactggtggt tcgaaggccc gcaatggatc gatttcctgc tgcgcaagat cgcccatgac
     1081 cagcacgaca tcaaaaccat cacgccgtcg gaatatctcg accgtttccc cgatctgcaa
     1141 cctgtcgctc cacccgagtg cagctggggt gcgggcgggt ttcacgaggt gtggctgaac
     1201 ggcgccaatg actggattta cccgtacctg cacagcgcca ccgagcgcat gagcgcgctg
     1261 gtaaggcgtt ttccgaacgc cgacggcctg ctgcgctggg cactcgatca ggcggcgcgt
     1321 gaattgctgc tggcacaatc gagcgactgg cctttcatca tgaaaaccgg caccagcgtg
     1381 gactacgccg tccgtcgttt caagactcat ctgcaccgct tccaccgcct caccgacatg
     1441 atccagtccg gcaactacga cgaaggctac ttgcgggaag tcaccgcacg cgacagtctg
     1501 ttccccgaca tggattaccg gatctttcag acgcggacat ggctatcctc gtctggttga
     1561 ggactgagtg cagcgtggat taatcctgat ggttgtcgcg cccatcgggt gggtccaggc
     1621 ccgatctctc cagttcttct cgtgaaatat gacgccagcc attctccgtt gcggcgtgca
     1681 tccaatatcc ggtgatgcca cagatcgtga cgcacagaac acggaacagt ggcgcaaaac
     1741 caaggtgcgc atgcgcctgg agcgagacat catttgtccg ctccgcccag ctgtaaaacc
     1801 gggtgttctc cggataccgg gaaagcgtct gatgaaaaag ccacgagaaa accccgtggt
     1861 gacgatgctc ctgagcgaca aacacattcc ccatgaaaac ttcattgttg tccaatgcga
     1921 acacgatgtt cttgcgccga tactcatcgt aggtcagcac atatttgtcc tcgaacgtcc
     1981 agcaatacgc cacgatcccg ccatcgtgtt tcatcacatg acagcgggca cgccggtcga
     2041 aaaactcgcc gaaatgtttg cggcgcgtcg cctgcgtggt cgcgccggaa cacgccagca
     2101 gctcatcgat cgcccgccga tccgcctgtt catagacgta gtcggagagc gcgtgcctcg
     2161 ctgcatgcga atcaagacca tccagccgca ggatctgagc ctcgttaaaa tacagcagcc
     2221 agccgggaaa atactccgac gccgtcaggc gcaacagcac ttcgtatact ccaccctgcc
     2281 ggaaatagtg aacgagcttt ttgagcttgg cggaaatcat ggcatgaaga aaacttttct
     2341 catgcacgat catactcgaa atcggggcgg gaataagtta ataacccgtc gcgtccgatt
     2401 ggtggcgtca tgcttcgata ttggccctgc ttgaaagcgt tactccaggc gtgttgcggc
     2461 cagcactgca ccgatcaggc cgacttcggg tctcgtcacc acctgcaccg gcaggtccgc
     2521 cacatacggt ttcatcttgc ccttgttaag aaacgcccgc atgaaccggc cgtcggtcag
     2581 ccttgaaatg attttcggcg caatgccgcc ggcaatataa acgccgcccg tggccccggc
     2641 ggtcaacgcc agattgcctg cctgcgcgcc gtagatatcc acgaacagat tcagcgtctc
     2701 attcgcgagc gcatcgttct gctccaacgc cgcctgggta atggctgccg ccggatcgcc
     2761 gtttcgcatc atcccggcca ccgacgggga ttcaggcata ttccctctcg ccttgaggaa
     2821 accataaagc tcaaccaggc cgtgaccgga cagaacaagc tcccaggaag tacggccggc
     2881 gtttttcaga agatagtgcg cgagctcgat ctgcaattca tttgctggtg caaagttggc
     2941 gtgtccacct tcggtggcaa ccggcatgta atggtcccgg ttccagaaca gcaacccttg
     3001 tccaagtccg gtgccggcgc cgattagggc gcgcggtcca tgcctgatcg ccttgccctt
     3061 ctgcaatacc gtcaggtctt ttttacccag cgcctcaata ccgtagccaa tggcctgaaa
     3121 atcattgacg aggagaagtc ggggtaattt gaaacggcgc ttgagctcct ggctgtgtat
     3181 ctcccagggc aggttggtga ccttgacgct ttgacccttt gccgtttccc ggaccggccc
     3241 ggcgacgccg aagcagacgg ctttgatatt tttttctctc tcttgtttca gaaattcgcg
     3301 aatgatgggc gacagactgt cataagacct gctgtcgaag cgctgcttgc gcacggcgcg
     3361 gcagcgaccc gccttgcatt ccgcgatttg cagcagtgtt tttgttccgc caatgtcgcc
     3421 cgccagtacg cgcatataaa atcgtcctcg gtgtcgcgga caggacatca cattgtgaga
     3481 acgatggcaa gattgctata ctggcgcgcc tcaagccggg gtggcggaac gggtagacgc
     3541 gccagactca atatctggtg gtggcgacac cgtgtcggtt cgattccgac cctcggtacc
     3601 aactaaaaaa gggccacagc gatgtggccc ttttctttta gccctaaaat ttcaatccta
     3661 cccctacctg tacaagtggg aagtggggct gatttaccag caaggtctgc ccttctccac
     3721 gctgttcttg accgtggcgt cctgcccgat ctcttttgtc ctcagctatc cacccgcgca
     3781 tccgttcccc gttcgagttg cacctgtagt ctgtcccgcc gtagacacgt tgtggcacgt
     3841 tgattccggt tgctgtctgt tgctggcact gcgcggggta cacgggggaa acggcgaaac
     3901 gatggaactg ctttacgatt ttgttactaa attgggctta ggtaacggga aagtgactcc
     3961 gaaaacgctc ttctaatgca ttaactaaat catctactgc tgcattgatg ccagcaataa
     4021 gctctccgcc aatagcttgt gcagagtgtt taggcgtatc gaaattgcga taggtaattg
     4081 cgcggcgcag cgcgattacc ttcatctctc gtgccaagtt attcaaaaga ttgagatggt
     4141 cgagagaata gaccaagcgc tgcaacataa ccttgactcg tcgcgcttcc gcttcagagt
     4201 gttcgggagc tgtatgatat tgctctgcct gcccttcaag ttcagaaagc gtggtccgca
     4261 gcttatcaag ctgtgcgcgt agttcctttc gcgcctcacg cttgttatgc tgccagttaa
     4321 caaccagcca gccgcatatt accagcgccc aagtaacaag ctggccgata gtgctaacaa
     4381 gctgcgaata atcagtcggc ccgctctgcg ccatgcacaa tgtaatcttc tatttcagca
     4441 ataatactgg aatctgtcga aacaaactta aacgtttttc tcaccaaatc ggggctataa
     4501 cccgccctaa ccaacccgcc aaacgcctct tcgagaaatg aagacccata accgcgtacg
     4561 ccgt
//
LOCUS       DBNGZE010000265         1520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_245579, whole genome shotgun sequence.
ACCESSION   DBNGZE010000265 DBNGZE010000000
VERSION     DBNGZE010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_245579"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..>1520
                     /locus_tag="AC16FV_06875"
     CDS             93..>1520
                     /locus_tag="AC16FV_06875"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - obsolete glycogen debranching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0019156 - isoamylase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HIR2849814.1"
                     /translation="MFDPYAEQLFFPPDFSREAARQPGGNDGRAVIGVLPHEEAEFNW
                     GTPPPRHTHDAVVYELHVKGFTARPNSGVAQEKRGTFLGLIEKIPYLKELGVTVVELL
                     PVHQFDPQEGNYWGYMTLGFLAPHKAYGVRDAAAEFRQMVRAFHEAGIEVWLDVVYNH
                     TAEGDEAGPTYAYRGIDNSSYYLVDQEGRYQDHSGCGNTTRCAHPSVRALVLRSLQHW
                     SERMGVDGFRFDLASVFMRNEDGSINTDSPAIAAEISAIAVELDLRLVAEAWDVDAYQ
                     LGRSFPGLAWRQWNGQFRDDVRSFVKGDAGMVGALMQRLYGSDDLFPDGPSDACRPYQ
                     SVNYVTAHDGFCLYDLVSYNQKHNEANGQQNHDGPADNRSWNCGWEGDAGAPAEVLSL
                     RRQQVKNFFALLMLANGTPMFCAGDEFLNTQRGNNNPYNQDNETTWLDWSLLEKNRDI
                     FLFFQHMIAFRKARPWIARSRYWRED"
BASE COUNT          335 a          403 c          476 g          306 t
ORIGIN      
        1 agaagtcagc gccggcggcg cggtattatg cctatcaagt ggaaggaccg tgggacccca
       61 aggacggaca tcggttcgac tcaaacaaaa tcttgttcga tccatacgcc gaacaactgt
      121 ttttcccgcc agacttttcg cgcgaagcgg cacggcagcc gggtggaaac gacgggcgcg
      181 cggtaatcgg agtgttgcct catgaggaag cggaattcaa ttggggcacg ccgccgccgc
      241 gacacacgca cgacgcggtt gtctacgagt tgcacgtcaa aggattcacg gcgcggccta
      301 actcaggagt cgcgcaggag aagcgcggca cgtttctagg actaattgag aagattccct
      361 acttaaagga gctcggtgtc accgtcgtgg aattattgcc ggtgcatcag ttcgatccgc
      421 aggagggcaa ttactggggc tatatgaccc tgggcttcct ggcgccgcac aaggcctatg
      481 gcgttcgcga cgcggcagcg gagtttcggc agatggtgcg ggcgtttcat gaagcaggca
      541 tcgaagtttg gttggatgtt gtttacaacc acactgccga aggcgacgag gccggaccca
      601 cctatgcgta tcgcgggatc gataactcca gctactatct ggtggaccag gaagggcgtt
      661 accaggacca cagcggctgc ggaaacacga cccgctgtgc gcacccgagc gtgcgcgccc
      721 tcgtattgcg cagcttgcag cattggagtg agcgaatggg cgtggacggg tttcgtttcg
      781 acctggcgtc ggtgttcatg cgcaatgaag acggcagcat caacacggat tcgcctgcca
      841 tcgccgctga aatcagcgcg atagcggtgg agcttgattt gcgtcttgtt gcggaggcgt
      901 gggacgtcga tgcataccag ctggggcgga gtttccccgg actggcgtgg agacagtgga
      961 acgggcaatt ccgcgatgat gtgcgctcgt ttgtcaaagg cgacgccgga atggtcggtg
     1021 cgttgatgca gcgactgtac ggcagcgacg atttattccc cgatggtccg agcgatgcgt
     1081 gccggcctta tcaaagcgtt aactacgtaa ccgctcacga cggcttttgt ctctatgatc
     1141 tcgtgtctta caaccagaaa cataacgagg cgaatggtca gcaaaatcac gatggcccgg
     1201 ctgacaatcg aagttggaac tgcggctggg aaggcgacgc cggcgcgcct gccgaagtat
     1261 tgtcgttgcg gcgccagcag gtgaagaact tcttcgcgct gctgatgctg gcgaacggga
     1321 cgccaatgtt ttgcgccggc gacgagtttc taaacacgca acgcggcaac aacaatccat
     1381 acaaccagga caacgaaacg acatggctcg actggtcctt actggaaaag aaccgggata
     1441 tatttctttt ttttcaacac atgattgcgt tccgaaaagc gcggccatgg atcgcacgca
     1501 gccgctattg gcgcgaggat
//
LOCUS       DBNGZE010000266         2268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_870677, whole genome shotgun sequence.
ACCESSION   DBNGZE010000266 DBNGZE010000000
VERSION     DBNGZE010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_870677"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>2268)
                     /locus_tag="AC16FV_06880"
     CDS             complement(177..>2268)
                     /locus_tag="AC16FV_06880"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR2849815.1"
                     /translation="SPTSDALITGRNHHSSGFGVVSEISTGFPGYDSIITRDKATIGT
                     ILREHGYATSWFGKNHNTPSYQISTAGPFDQWPSGMGFDYFYGFMGGETSQWTPYLYQ
                     DHTQIFPWIGKSDWNLTTAMADDAIRYMRELNAAAPDQPFFLYYVPGGTHSPHHPTKE
                     WIEKFKGKFDMGWNAMREQIFANQKRLGVIPANAQLTAWPNELPTWDSLSAEQKKLYA
                     REAEVFAAYVAYTDHEIGRVIQEVENLGKLDNTLIIYISGDNGTSAEGTMTGAFNTYL
                     GYNGVTEVPMAANMAHYDGWGLPGTDPHMAVPWAWAFDTPFKWTKQIASHFGGTRQGM
                     AISWPAVIKDKGGIRTQFHHMIDIVPTLLDATRIKAPDTVDGIKQAPIEGVSMMYTFD
                     KANTNVPSRRTTQYFEMGGYRGIYHDGWYAATTPPIAPWSPVLNMTLPDVLTGYKWEL
                     YNLTDDYSQANDLAAKNPDKLAELQKVFLQEAAKYQVFPLDNRAFVRVLTPRPSATAG
                     ISEFTYTGEISGIPAGNAPPFLGRSFTITADIQVPASGVEGMLATQGGETSGYGFYVV
                     DGKPVFTYNLLGMERFRWAASAPLTPGKHSVAFEFTYDGPGLAKGGTGVLKVDGAEAE
                     TKKIPRTTPFTMPFDETFDVGVDTRTGVNNADYRPPFRFTGTIDKLTFKVGPAQFTSN
                     DQQVIQHALSKARD"
BASE COUNT          473 a          625 c          635 g          535 t
ORIGIN      
        1 atttatcttc ctcaatgcgc accattgcaa taaagattca cccgcctctg ccacccgccg
       61 ccagcgtcga tcatctgcac cgctatatcg agcttttgtt tgagcccgca aagcgcagag
      121 gaaaggaaat ctcatgagcg caaacaacac atttaaatga ctacaaaacg tcgttgttaa
      181 tctcgtgcct tgctgagcgc gtgctggatg acctgctgat cgttgctcgt aaattgcgcc
      241 ggtccgacct tgaaagtcag cttgtcgata gtgccggtga aacggaacgg cggccggtag
      301 tcggcattat tcactccggt tcgagtatcg acaccaacgt cgaaggtctc atcgaacggc
      361 atggtgaaag gagtggtgcg cggaatcttt ttggtttcag cttccgcgcc atcgaccttt
      421 aggacacctg tgccgccttt ggcgagccct ggaccgtcat aagtaaattc aaaggcgact
      481 gaatgtttgc ccggcgtcag cggcgctgat gccgcccagc ggaaacgctc catcccgagc
      541 agattgtagg tgaacaccgg cttgccatcg acgacataga agccgtaccc gctggtttcg
      601 ccaccctgcg tcgcaagcat gccttcaaca ccactggccg gcacctggat gtcggcagtg
      661 atggtgaatg agcgacctag gaacggtggt gcattgcccg ctggaatccc ggaaatctcg
      721 ccggtgtaag taaattcgct gattccggcg gtcgcgcttg gccgtggcgt caggacgcgc
      781 acgaaggctc ggttgtcaag cgggaagacc tggtacttcg ccgcttcctg taaaaagact
      841 ttctgaagct ctgcaagctt gtctgggttc tttgctgcga gatcattcgc ctgcgaatag
      901 tcgtcggtta agttgtacag ctcccatttg tagccggtaa gcacgtcggg caaggtcata
      961 ttgagcacgg gtgaccacgg cgcaatcggc ggggtagtcg ctgcatacca gccatcgtga
     1021 taaatcccgc ggtagccacc catctcgaaa tactgcgtcg tacgacggga cggcacattc
     1081 gtattggcct tatcgaaggt gtacatcatg ctcacaccct cgatcggcgc ctgtttgatg
     1141 ccatcaaccg tatctggcgc tttaattcgc gttgcgtcca ggagcgtcgg cacgatatca
     1201 atcatgtgat ggaattgcgt gcggataccg cccttgtctt tgatgaccgc cggccacgag
     1261 atggccatgc cctgccgagt gccgccaaag tgtgaggcga tctgcttggt ccatttaaag
     1321 ggcgtatcaa atgcccaggc ccacggcacc gccatatgcg gatcagtacc gggcaagccc
     1381 cagccgtcgt aatgcgccat gttggcggcc atggggactt cggtgacgcc gttgtatccc
     1441 agataggtat tgaacgcgcc ggtcattgtt ccttccgcgc tcgttccatt gtcaccagaa
     1501 atgtaaatga tcagtgtgtt atccagcttg ccaaggttct cgacctcttg gattacgcga
     1561 ccgatctcgt gatcagtata ggcgacgtag gccgcaaaca cctcggcttc gcgggcgtaa
     1621 agtttcttct gttcagcgct caaactatcc caggtcggga gttcgttcgg ccacgcggtt
     1681 agttgggcgt tggcgggaat gacgcctaac cgcttctggt tcgcgaagat ctgttcgcgc
     1741 atggcattcc accccatgtc gaacttaccc ttgaattttt cgatccactc cttcgtcgga
     1801 tggtgaggcg aatgagtgcc gccgggcacg taatagagga agaagggttg gtcgggcgca
     1861 gccgcgttca gctcccgcat gtagcggatg gcgtcgtcag ccatcgccgt ggtgagattc
     1921 caatcggact tgccgatcca cgggaaaatt tgtgtgtggt cctgataaag atacggcgtc
     1981 cattggctgg tttcgccgcc catgaaaccg taaaaataat cgaaacccat tcccgacggc
     2041 cactgatcga acggacctgc ggtgctgatc tggtacgacg gcgtattgtg atttttcccg
     2101 aaccatgatg tggcgtaacc gtgttccctg agaattgtgc cgatggtcgc cttgtctcta
     2161 gtgatgatgg aatcgtagcc gggaaaaccc gtcgatattt cactcacgac accaaaaccg
     2221 ctggaatgat gatttcttcc ggtgatcagt gcgtcactgg ttggcgag
//
LOCUS       DBNGZE010000267        12389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1009822, whole genome shotgun sequence.
ACCESSION   DBNGZE010000267 DBNGZE010000000
VERSION     DBNGZE010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1009822"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="pyk"
                     /locus_tag="AC16FV_06885"
     CDS             complement(<1..917)
                     /gene="pyk"
                     /locus_tag="AC16FV_06885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR2849816.1"
                     /translation="MLRRTKIVATIGPATEDPKVIDKLIEAGVDVVRLNFSHDRHEVH
                     VRRAETVRERAKAHEREIGVVVDLQGPKIRIGKFRNGPIKLAEGETFVLDVDHPLDAG
                     NQERVGVTYKALPQDVERGVTLLLDDGRIVLWVDKVDGSAVICRVVIGGELSNSKGIN
                     RQGGGLTAKALTDKDREDIRVAAQMHADYVAISFPRSADDVNEARQLLRAAGGHGGII
                     AKIERAEAVQNIEAIIDAADAIMIARGDLAVEMGDAAVPPIQKRLIRMARERNKVSIT
                     ATQMMESMIEHAIPTRAEVSDVANAVIDGT"
     gene            complement(974..2155)
                     /locus_tag="AC16FV_06890"
     CDS             complement(974..2155)
                     /locus_tag="AC16FV_06890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR2849817.1"
                     /translation="MSVIKLTDLDLKGKRVLIRSDLNVPVKDGKVTSDARISASLPTF
                     NHCLKAGAKVMVMSHLGRPTEGEYSEENSLKPVADDLSKKLGKPVRLVRDWVNGGFDV
                     AAGELVLLENVRFNKGEKKNVDETAQKYAKLCDVFVMDAFGTAHRAEGSTHGVAKFAP
                     VACAGILLTEELEALTKALLNPKRPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGI
                     ANTFLAAAGKKIGKSLAEADLIPTAKTLMEKMQKRGATIPIAVDVVVGKKFDANEKAT
                     LKDAGSVADDDMIFDIGPKSAQELADIIMKAGTVVWNGPVGVFEFDQFGAGTKKIADA
                     IANTKAFTLAGGGDTIAAIQKYNIYDKVSYISTAGGAFLEFLEGKKLPAVAMLEERAK
                     K"
     gene            complement(2274..3272)
                     /gene="gap"
                     /locus_tag="AC16FV_06895"
     CDS             complement(2274..3272)
                     /gene="gap"
                     /locus_tag="AC16FV_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR2849818.1"
                     /translation="MAIKVGINGFGRIGRMVFRAVAKDFKDIEIVGINDLLEPNYLAY
                     MLKYDSVHGRFNGDIKVEGNNLIVNGRKIRLTAVKDPAELKWNEVGADIVVESTGLFL
                     TKETCEKHLAAGAKKVVQSAPSKDDTPMFVYGVNHAKYAGEKIVSAASCTTNCLAPVA
                     KVLHDNFGIKRGLMTTVHAATATQKTVDGPSSKDWRGGRGILENIIPSSTGAAKAVGK
                     VLPELNKKLTGMAFRVPTSDVSVVDLTVELNKEASYEQICAAMKKASEGELKGVLGYT
                     DEKVVSTDFRGNSMPSVFDAEAGIALDPTFVKVVSWYDNEYGYTCNMMRFVQHVAK"
     gene            complement(3398..5440)
                     /gene="tkt"
                     /locus_tag="AC16FV_06900"
     CDS             complement(3398..5440)
                     /gene="tkt"
                     /locus_tag="AC16FV_06900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR2849819.1"
                     /translation="MTVETQAPKAGKGKTLTNRRELANAIRALSMDAVQKANSGHPGA
                     PMGMADIAEVLWNDFLKHNPANPKWADRDRFVLSNGHGSMLLYSLLHLTGYDLPMEEI
                     KNFRQLHSKTPGHPEYGYAPGVETTTGPLGQGITNAVGMAIAEKVLAGTFNRNGHHIV
                     DHYTYVFLGDGCLMEGISHEACSLAGTLGLGKLIAFYDDNGISIDGHVEGWFTDNTPK
                     RFEAYGWHVVRDVDGHDADAIRRAILEARSVNDKPSMICCKTVIGYGAPNLCGSHDCH
                     GAPLGNDEIKATRENLGWNYEPFVIPTEIYAGWDAKSKGAAAEKSWNDKFAAYKKDHP
                     ELAAEFERRVVKGELPKDWAAKSAEFIKSVDAKAETIASRKASQNTLNGYGPVLPEFL
                     GGSADLAGSNLTIWKGSKGISNTVSDGNYVYYGVREFGMSAIMNGIALHGGFIPYGAT
                     FLMFSEYARNALRMAALMKIQSIFVYTHDSIGLGEDGPTHQAVEQTATLRLIPNMSVW
                     RPCDAVESAVAWKFAVERKKGPSCLIFSRQNLPHQKRDAQQLAAISRGGYVLSEAEGG
                     KPQAVIIATGSEVGIAMEALKLLAAKGKKVRVVSMPSTDAFDAQDAAYRESVLPKGVK
                     RVAVEAGVTAIWHKYVGLEGRVVGLDRFGESAPAGALFKHFGFTGENIAKTVEEIL"
     gene            complement(5792..6607)
                     /locus_tag="AC16FV_06905"
     CDS             complement(5792..6607)
                     /locus_tag="AC16FV_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR2849820.1"
                     /translation="MTLPSLETLREIVRAASREELMPLFADVIRHIKHDGSVVTEADV
                     AMQHRLKSDLAHHWPEYDFLGEEMPGHEHEQLTAGGQGLWCVDPLDGTSNFATGIPYF
                     AVSLALIIDGRQELGMVYDPVRDECFMARRGGGARLNEMTLGVHEPPLPDVLRRCVAS
                     VDFKRLQASLAAQLGAYPPYGSQRNFGASSLEWCWLADGRFHVYLHGGQKLWDYAGGS
                     LILAEAGGMATTLEGEKIFSYGLEPRSVVAAATPALFSVWKSWIDANWKPTRN"
     gene            complement(6604..7017)
                     /locus_tag="AC16FV_06910"
     CDS             complement(6604..7017)
                     /locus_tag="AC16FV_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849821.1"
                     /translation="MIVEETFYRPPELSREPRTLPAETYNLAHVLLKRATAGCLFVPI
                     RSMQFLAVVDGEEFIFVDREGKRMIELAWQHFAPQGRSSLEEPVFYEAVYYSPVAAEI
                     MRRIQGEFHKALRDLEQKSVHGGRARVIPLNGTSR"
     gene            complement(7175..7729)
                     /locus_tag="AC16FV_06915"
     CDS             complement(7175..7729)
                     /locus_tag="AC16FV_06915"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2849822.1"
                     /translation="MVRTETPVCDFGRPAPDFRLQGVDGKIWTLNEARGKSGLLVMFI
                     CNHCPYVKSVRDRIIRDARELKDHGINSVAIMSNDPADYPEDSFDNMKQVAQELNFPF
                     PYLYDETQEIAKAYGAVCTPDFFGYNRDLKLQYRGRLDESRKEPVPNARRDLFEAMKQ
                     ISQTGQGPKDQIPSMGCSIKWKIA"
     gene            7791..9137
                     /locus_tag="AC16FV_06920"
     CDS             7791..9137
                     /locus_tag="AC16FV_06920"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HIR2849823.1"
                     /translation="MDNDSLTQRDLRVLWHPCTQMRDHETLPLIPIRRGRGVWLEDFN
                     GKRYLDAISSWWVNLFGHANPRINTAIENQLQNLEHVILAGFTHEPVVRLSERLIEIA
                     PHGLAHCFYASDGSAALEIALKASFHYWQNRGEKKRTRFISLANSYHGETLGALSVGD
                     VALYKETYRPLLLEAITVKSPDCYHRETGESCADYSTRAFAEMEKALERHAGEVCAVV
                     VEPLVQCAGSMRMYDPVYLKLLRQSCDRHHVHLIADEIAVGFGRTGTMFACEQAGITP
                     DFLCLGKGLTGGYLPLSACLATDEIYQAFYADYAARKAFLHSHSYTGNPLACSAALAT
                     LDIFRDDDVINHNRRLAAHTAQATAALAEHPHVAEVRQRGMILAIEMVKNKKTREPFP
                     WQERRGLAVYRHALEQGVLLRPLGDVIYFMPPYVIKPEEIDFLVKVAQAGIDRAVA"
     gene            complement(9141..9602)
                     /locus_tag="AC16FV_06925"
     CDS             complement(9141..9602)
                     /locus_tag="AC16FV_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR2849824.1"
                     /translation="MKKPRSGPRLHSLEVPLEGMTLLVPSAAVAEVINPQSMAPVPFS
                     QPWLNGAIGWRTLAVPVISFESLLGAAQTSRPASGKIVIFYPLAGRKDWEFFAMFATA
                     EPRPQVLDGSQPVAQASELPDSPYIAAGLKLDGRLMLVPDLDAMKKVFYPA"
     gene            complement(9602..10045)
                     /locus_tag="AC16FV_06930"
     CDS             complement(9602..10045)
                     /locus_tag="AC16FV_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR2849825.1"
                     /translation="MADPNQYLRLQLGETSYLLPSASGFTIEQREHLIVNKSPEGNIA
                     AWQSLRSTRMPAYCLDGMLRVTRHHDWHRAVFLDAAPHAVGLVVDEVQLLPRSETVVS
                     PFTPLGLPPTRMGHLFSGAWVTGNRIVLVFDPKAFAAYLQSLGDA"
     gene            complement(10074..>12389)
                     /locus_tag="AC16FV_06935"
     CDS             complement(10074..>12389)
                     /locus_tag="AC16FV_06935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR2849826.1"
                     /translation="SRAQVAELKRSLHTLKGGARMAGAMAMGNLSHNTETLLKNIEDS
                     KVSASDEVFDLLDEAHDMLVAMLDGARDGKPVPDTSVLNAKLVALAFGQPLPAVIEAP
                     APAAEADNAPSEEPVAEPAQVAAEITQDVEAAAPAMAAAIDEVDERRDQLETEEKQWP
                     EKMERRGQVRVNTTLLNDLVNYAGEVSLSRSRMEQQIYSFRDNLGELSRNIVRFRDQI
                     RELEIQSESQILYRMEHEGAEGRTEDFDPLEFDRFSRLQQLSRSLAESLHDLGTIQNN
                     LGNFVGEAEAVLQQQARINTELQEGLMRTRMVSFSTQAARLRHIVRQTARELGKRVEL
                     NLENAEVELDRNVLERMIGPFEHMIRNSIDHGIESEAERRRKGKPPHGRINIATSQEG
                     SEVIIRFSDDGAGLNIEGIRAKAIERGLMPADANPSEEELIQFILMAGFSTAEKITHV
                     SGRGVGMDVVHSEVKQLGGSMSVDTEPGVGTTFIIRLPLTLSITQALMVYVGDQLFAV
                     PLGSVVNIVEFPVEKIAGISMGKNPLLSHNEQVYPYMHLGQRLNIVSQPRNGKKVPVL
                     LARTGTREVAIQVDGLGGTREVVIKSVGPQLSEIKGLAGATILGDGRVILILDVPGLW
                     FRDDVIHFEHRPEGKVAQEVRARPVVMVVDDSLTVRKVTSKHLQKRGMDVLVAKDGLD
                     AVEQLRDHLPDVMLVDIEMPRMDGYELTTRVRSDENLKHIPIIMITSRAGAKHRQKAF
                     ELGVDMYMRKPYHEDELFKNIDTLLAKGRLT"
BASE COUNT         2256 a         3889 c         3683 g         2561 t
ORIGIN      
        1 gtgccgtcga tcacggcgtt ggcgacatcg gacacctcgg cgcgcgtcgg gatggcatgt
       61 tcgatcatcg actccatcat ctgcgtcgcg gtgatcgaaa ccttgttgcg ttcgcgcgcc
      121 atccggatga gccgtttctg tatcggcggc acggccgcgt ctcccatctc gaccgcgaga
      181 tcgccgcgcg cgatcatgat cgcgtccgcg gcatcgatga tggcctcgat gttctgaacc
      241 gcttccgcgc gttcgatttt ggcgatgatc ccgccgtgac cgccggccgc gcgcaacagc
      301 tgccgcgcct cgttcacgtc atcggcgctg cgcgggaagg agatggcgac gtaatcggcg
      361 tgcatctggg cagccacgcg gatgtcctcg cggtccttgt cggtcagcgc cttggcggtg
      421 agcccgccgc cctggcgatt gatgcccttg ctgttcgaga gttcgccacc gatcaccacg
      481 cggcagatca ccgcgctgcc atcgaccttg tccacccaca gcacgatgcg cccgtcgtcg
      541 agcaacaggg tcacgccgcg ttcgacgtcc tgcggcagcg ccttgtaggt gacgccgacg
      601 cgctcctggt tgccggcatc gagcggatga tccacgtcca ggacgaaggt ttcgccttcc
      661 gccagcttta tcgggccgtt cctgaatttg ccgatgcgga tcttcggacc ctgcagatcc
      721 accaccacgc cgatttcacg ctcgtgcgcc ttggcacgct cgcgcacggt ctcggcgcgg
      781 cgcacgtgaa cttcgtgccg gtcatgagag aaattgaggc gcaccacgtc cacaccggcc
      841 tcgatcaact tgtcgatgac cttgggatcc tccgtggccg gaccgatggt ggcgactatt
      901 ttagttcgac ggagcaaatt aactccttgt atggctggaa gtcgcaaagg cttggccgcg
      961 ggttccactt gacctatttt ttggcgcgct cttccagcat ggcgaccgcc ggcagttttt
     1021 tgccctcgag gaattccagg aatgccccgc cggccgtgga gatgtaggac accttgtcgt
     1081 aaatattgta tttctggatc gcggcgatgg tgtcgccgcc gcccgccagg gtaaatgcct
     1141 tggtattggc gatggcgtcg gcgatctttt tggtgccggc gccgaactgg tcgaactcga
     1201 acacgcccac cgggccgttc cacaccacgg tgccggcctt catgatgata tcggccagct
     1261 cctgcgcgct cttggggccg atatcgaaga tcatgtcgtc atcggccacg ctgcctgcgt
     1321 ccttgagtgt cgccttttcg ttggcatcga atttcttgcc caccaccacg tccaccgcga
     1381 tggggatcgt ggcgccacgc ttctgcattt tttccatcag cgtcttggcg gtcgggatga
     1441 gatcggcttc cgccagcgac ttgccgatct tcttgccggc cgcagccaag aaggtgttgg
     1501 cgatgccgcc gccgaccacc agttgatcga ccttctcgga cagcgattcg agtacggtca
     1561 gtttggtcga gaccttggaa ccgccgacga tcgctaccat cgggcgcttc gggttgagca
     1621 gcgccttggt gagcgcctcc aactcttccg tcagcaggat gccggcacag gcaaccggcg
     1681 cgaacttggc gacaccgtgg gtcgagccct cggcgcggtg cgcggtgccg aacgcgtcca
     1741 tcacgaacac gtcgcacagc ttggcgtact tctgcgcggt ctcgtcgacg ttcttcttct
     1801 cgcccttgtt gaaacgcaca ttctcgagca gtaccagctc gccggcagcg acatcgaaac
     1861 cgccgttgac ccagtccctg accaggcgta ccggcttgcc gagcttcttc gacagatcgt
     1921 ccgccaccgg cttaagcgaa ttctcttccg aatattcacc ctcggtcgga cggccgagat
     1981 gcgacatcac catcaccttg gcgccggcct tgaggcagtg attgaacgtc ggcaacgaag
     2041 cgctgatgcg cgcgtcggac gtaaccttgc cgtccttcac cgggacgttc aggtcggaac
     2101 ggatcaggac gcgcttgccc ttgagatcga gatcggtcag cttgatgacg gacatatttt
     2161 aaaccctaaa agattcttga accgccaagg cgccaagagc gccaggaaaa accagaatta
     2221 atttccaatt tcccgattct tggcgtcctt ggcgtcttgg cggtgaaatt tttttatttg
     2281 gcgacgtgct gcacgaaacg catcatgttg caggtgtagc cgtactcgtt gtcgtaccag
     2341 gacacgacct tgacgaaggt cgggtcgagc gcgatgccgg cttcggcgtc gaaaaccgac
     2401 ggcatgctgt taccgcggaa gtcagtagac accacctttt catcggtgta tccgagtacg
     2461 cccttgagct caccttccga ggccttcttc atggccgcgc agatttgttc gtagctggct
     2521 tccttgttca gttccacggt caggtcaacg acggacacgt ccgaagtcgg cacgcggaag
     2581 gccatgccgg tgagcttctt gttgagctcc ggcagcacct tgccgacagc tttggcggca
     2641 ccggtcgagg acgggatgat gttttccaga atgccgcggc cgccgcgcca gtccttgctg
     2701 gacgggccat cgacggtctt ctgggtggcg gtggcggcgt gcaccgtggt catcaggccg
     2761 cgcttgatgc cgaagttgtc gtgcagcacc ttggccaccg gggccaggca gttggtggtg
     2821 caggaagcgg cggaaacgat tttctcgccg gcatatttgg cgtggttcac gccgtatacg
     2881 aacatcgggg tgtcatcctt cgacggtgcc gactgtacga ccttcttggc gccggcggcg
     2941 aggtgcttct cgcaggtttc cttggtcagg aacagaccgg tggactcgac gacgatgtcg
     3001 gcgcccactt cgttccactt gagttcggcg ggatccttga cggcggtcag gcggattttc
     3061 ctgccgttga cgatcaggtt gttgccctcg accttgatgt cgccgttgaa gcggccgtgc
     3121 acggaatcgt acttcagcat gtaggcgaga tagttgggct ccagcaggtc gttgatgccg
     3181 acgatctcga tgtccttgaa atctttcgcg accgcgcgga ataccatgcg tccgatgcgt
     3241 ccgaatccgt taataccgac tttgattgcc ataacccaat ctccagaaag ttaattaaaa
     3301 actatataaa ccgccaagac gccagggacg ccaagaaaag caatttttaa atttaaaaaa
     3361 acttggcgct cttggcgtct tggcggttca aaattagtta caaaatttct tccaccgtct
     3421 tggcgatgtt ctcgccggtg aagccgaagt gcttgaacag cgcgccggcc ggggcggatt
     3481 cgccaaagcg gtccaagccc accacgcgac cttccaggcc cacgtacttg tgccagatgg
     3541 cggtcacgcc ggcctcgaca gcgacgcgct tcacaccctt gggcagcacg ctctcgcggt
     3601 aagccgcatc ctgagcatca aaagcatccg tggaaggcat cgacaccaca cgcaccttct
     3661 tgcccttggc ggcgagcagc ttcagcgcct ccatggcgat gccgacttcg gaaccggtgg
     3721 cgatgatcac ggcctgcggc ttgccgcctt cagcctcgga caacacataa ccgccgcggg
     3781 aaatcgccgc gagttgctgg gcgtcgcgct tctggtgcgg cagattctgg cgcgagaaga
     3841 tcagacacga aggccccttc ttgcgctcga cggcgaattt ccaggccacc gcgctctcga
     3901 cggcgtcaca gggacgccac acggacatat taggtatgag tcgtagcgtc gcggtctgct
     3961 cgaccgcctg gtgcgtcgga ccgtcttcgc cgagaccgat ggagtcgtgg gtgtagacga
     4021 agatcgactg tatcttcatc agcgccgcca tgcgcagcgc attgcgtgcg tactcggaga
     4081 acatcaggaa ggtagcgccg taggggatga agccgccgtg cagggcgatg ccgttcatga
     4141 tcgccgacat gccgaactcg cgcacaccat aatagacgta gttgccgtcc gaaacggtgt
     4201 tgctgatgcc cttcgagcct ttccagatgg tcaggttgga gccggcgagg tcggcggaac
     4261 ctccgaggaa ctccggcaac accgggccgt agccattcag ggtgttctgc gaggccttgc
     4321 gcgaggcaat ggtctcggcc ttggcgtcca cggatttgat gaactcggcg ctcttggcgg
     4381 cccaatcctt cggcaactcg cccttcacca cgcggcgctc gaactcggcg gccagttccg
     4441 ggtggtcctt cttgtaggcc gcgaacttgt cgttccagga tttctcggcg gcggcgccct
     4501 tcgatttcgc gtcccagccg gcgtagatct ccgtcgggat tacgaagggc tcataattcc
     4561 agcccaggtt ttcgcgggtg gccttgatct cgtcattgcc gagcggggcc ccgtggcagt
     4621 cgtgactgcc gcacaggttg ggcgcgccgt aaccgatcac ggtcttgcag cagatcatgg
     4681 acggcttgtc gttgaccgaa cgtgcctcga ggatcgcgcg ccggatggcg tcggcatcgt
     4741 ggccatcgac atcgcgcacc acgtgccagc cataggcctc gaagcgcttc ggggtattat
     4801 cggtgaacca gccctcgaca tgaccgtcga tcgagatgcc gttgtcgtcg taaaacgcga
     4861 tcagcttacc cagacccagc gtgcccgcga gcgagcaggc ctcgtgcgag atgccttcca
     4921 tcaggcaacc gtcgccaagg aatacataag tataatgatc gacgatgtga tgaccgttgc
     4981 gattgaaggt cccggcgagc accttctcgg cgatcgccat gccgacggcg ttggtgattc
     5041 cctgtcccag cggaccggtg gtggtctcga cgcccggcgc gtagccgtat tcgggatggc
     5101 ccggggtttt ggaatggagc tggcggaaat tcttgatttc ctccatcggc aggtcgtaac
     5161 ccgtcaggtg cagcaggctg tacaacaaca ttgagccgtg gccgttcgac agcacgaagc
     5221 ggtcgcgatc cgcccacttc gggttggccg ggttgtgctt gaggaaatcg ttccacaaga
     5281 cttcggcgat gtcggccatg cccatgggcg cgccgggatg gccggagttg gccttctgca
     5341 ccgcgtccat ggaaagcgcg cggatggcgt tcgccaactc acgccggttg gttaatgtct
     5401 tgcctttgcc cgccttgggg gcctgggttt ctaccgtcat gggttaatcc tcaatgctcg
     5461 aaatcttgcc gacccgaaat ggtctgtcgc cgttccgtgt ccgtcgtggg cgccggttcc
     5521 aaaaaaaata acgggccccc ctgcgccatt gggctggtca gtcaccaaag ggagcggaaa
     5581 ccggatacgc ctgatgattc tttctgtctt aaaaagcgcc gttacgtgtt gtacacggcg
     5641 accggggcct tggcacagct ccttaaaccg cgcgcatttt cgctcacggt cggtctttgg
     5701 gcaatagccg aaaacggtcc ctgctcattc cgttatccgc ccataaacag gtttttgtgc
     5761 atgaagcggt tattccgtaa gggataaaca atcaattgcg cgtcggcttc cagttggcgt
     5821 cgatccagct cttccaaacc gaaaataaag ccggcgtagc cgccgccacg accgaacggg
     5881 gctcaagtcc gtaactgaaa atcttttcgc cctcgagcgt ggtggccatg ccgccggcct
     5941 cggccaggat caggctgccg ccggcataat cccacagctt ctgcccgccg tgcagataca
     6001 catgaaagcg gccgtccgcg agccagcacc attccaggct gctggcgccg aagttgcgct
     6061 gcgaaccgta aggcggatac gcccccagtt gagcggccag cgacgcctgc aaacgtttga
     6121 aatccaccga cgccacgcag cggcgcagca cgtccggcag cggcggctcg tgcacaccca
     6181 gcgtcatttc gttcagtcgc gcgccgccac cgcgacgcgc catgaaacat tcgtcgcgca
     6241 ccggatcgta gaccatgccg agctcctgcc ggccatctat tatgagcgca agtgaaacgg
     6301 caaaataggg aatgccagtg gcgaagttgc tggtgccatc cagcgggtcc acgcaccaca
     6361 ggccttgtcc gccggcagtc agctgttcgt gctcgtgccc gggcatctcc tcgcccaaga
     6421 aatcatattc cggccagtgg tgtgcgagat cggatttcag ccggtgctgc atggccacat
     6481 cggcttccgt caccacgctg ccgtcgtgtt tgatgtggcg gatgacatcc gcgaacagcg
     6541 gcatcagctc ctcgcgcgat gccgcgcgca caatctcccg caaggtttcc agcgatggca
     6601 gcgtcatcgg ctcgtaccat tgagcgggat cacgcgcgca cggccgccgt gcacggactt
     6661 ctgctccaga tcgcgcaggg ccttgtgaaa ctcgccctgt atccgtcgca tgatctcggc
     6721 ggcaaccggc gagtaataca ccgcctcata aaaaaccggc tcttcgagcg agctccgtcc
     6781 ctgcggcgca aagtgctgcc acgccagctc gatcattctc ttgccctcac ggtcgacgaa
     6841 aatgaattcc tcgccatcca ccacggcaag aaattgcatg ctgcggatcg gcacgaacag
     6901 gcagccggcg gtggcgcgct tgagtaaaac atgcgcgagg ttataggtct ccgcgggcag
     6961 cgtgcgtggc tcgcgggaca gctccggcgg acgataaaag gtttcctcta ctatcatatg
     7021 aatttctgcc gcagagacgc agagtacgca gagagaatca ttttaaatac tgaatcacga
     7081 attcagagcc ggttgtattc actgttatcc tccggcattg aacattcaca attcatttta
     7141 ctctgcgttc tctgcgtctc tgcggcaaat aatcttatgc aatcttccac ttgatcgagc
     7201 agcccatgct cggaatctgg tccttcgggc cctgtccggt ctgcgagatt tgcttcatcg
     7261 cttcgaacag gtcacggcgc gcgttcggca ccggttcctt gcgcgactcg tccagtcgcc
     7321 cgcggtattg caatttcaaa tcccggttgt agccaaagaa atccggcgta cagacggcgc
     7381 cataggcctt ggcgatttcc tgggtttcat cgtacagata cgggaacggg aaattcagtt
     7441 cttgcgccac ctgcttcatg ttgtcgaagg aatcttccgg ataatccgcg ggatcgttcg
     7501 acatgatcgc caccgagttg atgccgtgat ccttcaattc acgcgcatcg cggatgatgc
     7561 gatcgcgcac ggatttgacg tagggacagt gattgcagat gaacatcacc agcaggccgc
     7621 tcttcccgcg cgcctcgttc agggtccaga tttttccatc gacgccctga aggcggaaat
     7681 ccggggccgg acggccgaaa tcgcacactg gggtttcggt acgcaccatg actgtctctc
     7741 cacgagcttc attaacatgg ggcaacgcgc cctgtatcat caggcagtct atggataacg
     7801 actccctgac gcaacgtgac ctgcgggtgc tgtggcatcc ctgcacacag atgcgcgatc
     7861 acgaaaccct tccactcatc cccatacgcc gcgggcgcgg cgtatggctg gaagatttca
     7921 atggcaaacg ctacctcgac gccatcagct cgtggtgggt caatctgttc ggccacgcca
     7981 atccacgcat caacaccgcc atcgagaacc agttgcagaa tctcgagcac gtgattttgg
     8041 cgggtttcac acacgaaccg gtcgtgcggc tctccgagcg actgatcgag atcgcgcccc
     8101 atgggctcgc gcactgtttc tacgcctcag acggttccgc ggcgctggag atcgccctta
     8161 aagcgagttt ccactactgg cagaaccgtg gcgagaaaaa acgcacgcgc ttcataagcc
     8221 tcgcgaacag ctatcacggt gaaaccttgg gcgcgttgtc ggtgggcgac gtcgcgttat
     8281 ataaggaaac ttatcgcccc ttattactgg aagcgatcac ggtcaaatcc ccggattgct
     8341 accaccgtga gaccggcgaa tcctgcgccg attattccac ccgcgcgttc gcggaaatgg
     8401 aaaaagcgct ggaacgtcac gccggtgaag tctgtgccgt ggtggtggaa ccgctggtgc
     8461 agtgcgccgg gtccatgcgc atgtatgatc cggtttatct aaaactgctg cgtcagtcct
     8521 gcgacaggca tcacgtgcac ctcatcgccg atgaaatcgc cgtgggcttc ggccgcaccg
     8581 gcacaatgtt cgcctgcgaa caggccggca tcacccctga tttcctgtgc ctcggcaagg
     8641 ggctgaccgg cggctatctg ccgctctcgg cgtgtctcgc gaccgacgag atctatcagg
     8701 cgttctatgc cgattacgcc gcgcgcaagg cgttcctgca ttcgcactcc tacaccggca
     8761 acccgctcgc ctgttccgcg gcgctcgcca cgctcgatat ttttcgtgat gacgatgtca
     8821 tcaaccataa ccggcggctc gctgcgcaca cggcgcaagc gacggctgcg ctcgccgaac
     8881 atccgcatgt ggccgaggtg cgccagcgcg gcatgattct ggcgatcgag atggtgaaga
     8941 acaaaaaaac ccgcgagcct tttccctggc aggaacggcg tgggttggcg gtttaccgcc
     9001 acgcgctgga acaaggcgtg ctgctgcgcc cgctcggcga cgtgatttat ttcatgccgc
     9061 cgtacgtgat caaaccggag gaaatcgatt ttctggtgaa ggtggcgcag gcggggattg
     9121 atcgcgccgt ggcctgaaac tcacgccgga taaaacacct tcttcatggc atccaggtcc
     9181 gggacgagca tcagccggcc atccagcttg aggccggccg cgatgtaagg cgagtccggc
     9241 agttcggagg cctgcgccac cggctgactg ccgtccagca cctgcggacg cggctcggcc
     9301 gtggcgaaca tggcgaagaa ttcccagtcc ttgcgcccgg caagcggata aaaaatgacg
     9361 atcttgccgc tcgccggcct ggacgtctgc gccgcgccca acagcgattc aaatgaaatc
     9421 accgggaccg cgagcgtgcg ccagccgatc gcgccattca gccacggctg gctgaaggga
     9481 accggggcca tgctctgcgg attgatcacc tccgccaccg cggcgctcgg aaccagcagg
     9541 gtcatgccct ccagtggaac ctccagcgaa tgcaggcgcg ggccgctgcg cggtttcttc
     9601 atcacgcgtc ccccaggctc tgcaaatacg cggcgaaggc cttgggatcg aaaaccagca
     9661 cgatgcggtt gcccgtcacc caggcgccgg aaaacagatg acccatgcgc gtgggcggaa
     9721 gcccgagcgg tgtgaagggc gagaccacgg tctccgaacg cggcagcaac tgcacctcgt
     9781 ccaccaccag cccgaccgcg tgcggcgccg cgtccagaaa caccgcgcgg tgccagtcat
     9841 gatgccgcgt gacgcgcagc atgccgtcca agcaatacgc cggcatgcgc gttgagcgca
     9901 gcgactgcca tgccgcgata ttgccctcgg gggatttgtt gacgatcagg tgctcgcgct
     9961 gctcgatggt gaagcccgag gcgctgggca agaggtagct ggtctctccc agttgcaggc
    10021 gcagatactg attcggatca gccaaggttt tttattcccg ggtatccagc gttttacgtc
    10081 aaccggccct tggcgagcag ggtgtcgatg ttcttgaaca actcgtcttc atgatagggt
    10141 tttctcatgt acatgtccac gcccagctcg aacgccttct gccggtgctt ggcgccagcg
    10201 cgcgaggtga tcatgatgat cgggatgtgc ttcaggttct cgtcggagcg cacgcgcgtc
    10261 gtcagctcgt aaccgtccat gcgtggcatc tcgatgtcga ccagcatcac gtcgggcagg
    10321 tgatcgcgca gttgctcgac ggcgtcgaga ccgtccttgg cgaccagcac gtccatgccg
    10381 cgtttctgca gatgcttgct cgtgactttg cgcacggtga gcgagtcgtc caccaccatg
    10441 accaccggcc gcgcccgcac ttcctgtgcc accttgcctt ccggacggtg ctcgaaatgg
    10501 atgacgtcgt cgcggaacca caggccgggg acgtccagga tcagtatcac gcgtccgtcg
    10561 ccgaggatgg tggcgccggc cagccccttg atctcggaca gttgcggacc gacggacttg
    10621 atcacgacct cgcgcgtgcc gccgaggccg tccacctgga tcgcgacctc gcgcgtgccg
    10681 gtgcgcgcca acagcaccgg caccttcttg ccgttgcgcg gttgcgaaac gatgttcagg
    10741 cgctgtccca ggtgcatgta cggataaacc tgctcgttgt gggacagcag cggattcttg
    10801 cccatcgaga tgccggcgat cttctctacc ggaaattcga cgatgttcac caccgaaccc
    10861 agcggcacgg caaacagctg gtcgcccacg tacaccatca gcgcctgggt gatggacagc
    10921 gtcagcggca gacggatgat gaaggtcgtg cccacacccg gctcggtgtc cacagacatg
    10981 ctgccgccca gctgtttcac ctcgctgtgc accacatcca tgccgacgcc gcggccggag
    11041 acatgcgtga tcttttccgc cgtcgagaat ccagccatga gaatgaactg gatcagttcc
    11101 tcttccgatg gattggcgtc ggcgggcatc agcccacgtt cgatcgcctt ggcgcggatg
    11161 ccctcgatgt tgagtcccgc gccgtcgtcg gaaaaacgaa tgatgacttc gctgccctcc
    11221 tgcgaggtgg cgatgttgat gcgcccgtgc ggcggcttgc ccttgcgacg gcgttcggcc
    11281 tcggattcga tgccgtggtc gatgctgttg cggatcatgt gctcgaacgg cccgatcatg
    11341 cgttccagca cgttacggtc gagctcgacc tcggcgtttt cgagattgag ttcgacacgc
    11401 ttgcccaact cgcgcgcggt ctggcgcacg atgtggcgca ggcgcgcggc ctgcgtggag
    11461 aaactcacca tgcgcgtgcg catcagacct tcctgcaact cggtgttgat gcgcgcctgt
    11521 tgttgcagca cagcctcggc ctcgccgaca aaatttccga ggttgttctg gatggtaccg
    11581 aggtcgtgca aactttccgc cagcgagcgc gacaactgct gcaggcggct gaaacggtcg
    11641 aactctagcg gatcgaaatc ctcggtacgc ccttcggctc cctcatgctc catgcggtag
    11701 agaatctgcg actcggactg tatttccagc tcgcgaatct gatcgcggaa gcgcacgatg
    11761 ttgcgcgaca gttcgcccag gttgtcgcgg aagctgtaaa tctgctgttc catgcgcgaa
    11821 cgcgagaggc tcacctcgcc cgcgtagttg acgagatcgt tcagcagggt cgtgttgacg
    11881 cgcacctggc cgcggcgctc catcttctcc ggccactgct tctcttcggt ttccagctgg
    11941 tcgcgacgct catcgacttc gtcgatggct gccgccatgg ccggtgccgc agcctcgaca
    12001 tcctgcgtga tctccgcggc cacctgcgcc ggttcggcga cgggttcctc gctgggcgca
    12061 ttgtccgctt ccgccgcggg cgccggtgcc tcgataacgg ctggcagcgg ctggccgaag
    12121 gccagtgcca ccagcttggc attcagcacg ctcgtgtcag ggacgggttt gccatcgcgc
    12181 gcgccatcca gcatcgctac cagcatgtcg tgcgcttcat ccagcaggtc gaatacttcg
    12241 tcgcttgccg ataccttgct gtcttcaatg ttcttaagca gcgtctcggt gttatggcta
    12301 aggttaccca tggccatggc gccggccatg cgcgcgccgc ccttgagcgt gtgcagcgaa
    12361 cgcttgagtt ccgccacctg cgcccggct
//
LOCUS       DBNGZE010000268         5002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1565727, whole genome shotgun sequence.
ACCESSION   DBNGZE010000268 DBNGZE010000000
VERSION     DBNGZE010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1565727"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="AC16FV_06940"
     CDS             <1..862
                     /locus_tag="AC16FV_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR2849827.1"
                     /translation="LISHIPFLWLIAGLLVAPAAVAVAPHGANTHNPPPASTTSNTSS
                     KFDEKAALTISQGAIGKNIGDYTLTAPDGRRVRLSEYRGKPLVISLIYTSCYHICPTT
                     TQHLAKVVRTARAALGPESFNVLSIGFDTPNDTPAAMRQFSLDQGIDMQGWLFLSTHA
                     QAMEGLTKELGFISYRAPHGFDHLIQATVIDAQGKIYRQVYGMTFETPLLVEQLKELV
                     FGTPATPSLLTSLSNKIKLFCTVYDPTTDKYRFDYSIFLGLLIGASSIGIVGFLVLRE
                     WRRTRRRQQG"
     gene            951..2546
                     /locus_tag="AC16FV_06945"
     CDS             951..2546
                     /locus_tag="AC16FV_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR2849828.1"
                     /translation="MLESLQRAGRRAFAFVENAFDAVFGPRFNPFYHLGALAFFFFWI
                     VAASGIYLFIFFETSIDGAYRSVEYLTHTQWYLGGVARSLHRYASDAMVVATLLHLAR
                     EFVLDRYRGVRWYAWITGVMLLWFLYASGMSGYWLVWDRLAQYIAVVTAEWLDWLPFF
                     GESIARNFLNSATVSDRFFSLLSFMHLGIPLFLLLGMWIHIQRISRAKASPPRAIMLG
                     TLAALIVLSLVYPALSQGPADLDTVVSMVRPDWFYLMLYPLIDAWSPGAVWIVAGGGT
                     LLRMLLPWLPRRPEEAAAVVSLPNCNGCGRCFADCPYSAVIMQPRTDGLPYDSQASVN
                     PDLCTACGICAGACPTATPFRRTTELVAGIDMPLLPVRVIRQRTLDAMARLSGNDRVL
                     IYGCRSGGDLTSLERSGVGVVTLPCVGALPPSFIDFAISRRHADGVILVGCAQGDCYQ
                     RLGIDWTEQRLAGQRDPYLRGRVPRNRVATIWAGPRDGAELARRVAAFRAQLRAMGPF
                     PQGGTPSDSSSQSEMQNVSYRMG"
     gene            2524..2916
                     /locus_tag="AC16FV_06950"
     CDS             2524..2916
                     /locus_tag="AC16FV_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR2849829.1"
                     /translation="MSLIEWDEKFSVGVAAVDHEHRELIGLVNETYDKLKRPDAGIAV
                     PDFLGEIYARISAHFALEEKLMRANRYAHLNEHKSDHERLLDEIRNIMDDYEDGRVFD
                     TEQFAQRLGAWFTEHFRTHDARLHRQLD"
     gene            3046..3552
                     /locus_tag="AC16FV_06955"
     CDS             3046..3552
                     /locus_tag="AC16FV_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849830.1"
                     /translation="MLKNILRYSGQAVFYGLFVAFIGYFSTAPAYVHFPSDQALIKVS
                     FSHAGQPLEECRVRTAEELAKLPPNMRVPTQCGRERSPLTFELELDGKPVYRAELPPR
                     GLSRDGASTVYQRFPVPAGQHRFRARLKDSVHVSDFNYTKEAEVTLAPGQVFVVDFNA
                     RAGGFVFK"
     gene            3604..4977
                     /locus_tag="AC16FV_06960"
     CDS             3604..4977
                     /locus_tag="AC16FV_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR2849831.1"
                     /translation="MNPSDFSLPVPDDARRNLARGWLLLGLASLLVSGVFSVLLVLAR
                     APYVQNFFPWVDFFHTALVVHVDLSVLVWFLAFGGVLWSLNSTPRFLGAGRLALLLAL
                     LGTLVMATAPFLGAGEALMSNYIPVLQHPLFLAGLLLFAGGTGVLVLRAMGAIPPVGQ
                     WMQGAAALRFGLNTAAVSAMLALMAFAWSYFAVPDFLRGKPYYELLFWGGGHVLQFTY
                     TLLMLVSWLWLASASNVRLALSPRVALIFFGFGLIAVFFTPIIYYSYTVTSVEHIKLF
                     TWLMQYGGSLATLPLSLALLHGLWRAERAQGDAQPLRAALIASMILFGAGGVIGFLIT
                     GTNVTIPAHYHGSIVGVTLSLMGLTYYLLPHFGYRRPDMKLGRLQPYLYGGGQLLHVL
                     GLVISGGYGVQRKVAGAEQALDSAGRIAGMGLMGLGGLISIAGGFLFIYVCYRAMWPK
                     RSLSAKP"
BASE COUNT          797 a         1482 c         1563 g         1160 t
ORIGIN      
        1 tttgatctcg catatcccct ttctttggct gatcgccggt ttgctcgttg caccggccgc
       61 cgttgcagtc gcaccgcatg gcgccaacac gcacaaccca ccaccggcgt cgacgacaag
      121 caatacgtcg tcgaagttcg atgaaaaggc cgcgctgacc atcagccagg gcgcgatcgg
      181 caagaacatc ggcgattaca cgctgacggc gccggacgga cgccgtgtgc gcctttcgga
      241 atatcgcggc aagccgctgg tgatcagcct catctacacc agttgctatc acatctgccc
      301 gaccacgacg cagcaccttg ccaaggtggt acgtacggcg cgcgccgcgc ttggaccgga
      361 gagcttcaac gttctcagca tcggtttcga cacgcccaat gacacgccgg cggcgatgcg
      421 ccagttttcc ctggaccagg gcattgatat gcagggctgg ctgttcctga gcacccacgc
      481 ccaggcgatg gaaggcctca cgaaagagct cggttttatt tcctatcgcg ccccgcacgg
      541 cttcgaccat ctgatccagg ccaccgtgat cgacgcgcaa ggaaagattt accgccaggt
      601 ttacggaatg accttcgaga cgccgctgct ggtggagcag ttgaaggaac tggttttcgg
      661 gacgccggcc acgccctcgc tgctcacgag cctgagcaac aagatcaagc tgttctgcac
      721 ggtctacgat ccgaccacag acaagtaccg cttcgattac tcgatttttc tggggctttt
      781 gatcggcgcc agttccatcg gcatcgtggg ttttctggtg ctgcgcgaat ggaggcgcac
      841 gcgccgtcgc cagcagggtt gacctgaatc atacgattgc cggtatttcc gggcacaatg
      901 tcagggtgtg tcatcgccgg ttctggtgat gagttttgtg gagacgggct ttgcttgaat
      961 cgctacaacg cgccggccgg cgggcgtttg cctttgtcga gaacgccttc gatgccgtct
     1021 tcggtccccg gttcaacccg ttttatcacc tcggggcgct ggcgtttttc tttttctgga
     1081 tcgtggcggc cagcggcatt tatctcttca tctttttcga gaccagcatc gacggcgcct
     1141 accgttcggt cgaatacctg acgcataccc agtggtacct gggcggggtt gcgcgcagcc
     1201 tgcatcgcta tgcctcggat gccatggttg tcgccacgct gctgcatctg gcgcgtgagt
     1261 tcgtgctgga tcgctaccgc ggggtgcgct ggtatgcctg gatcacgggg gtgatgctgt
     1321 tgtggtttct gtacgcctcg ggtatgagcg gttactggct ggtgtgggac cgtctggccc
     1381 agtatatcgc ggtggtgacc gcggaatggc tcgactggct gccgttcttt ggcgaatcca
     1441 tcgcgcgcaa ttttcttaac agcgcgacgg tcagcgaccg cttcttctcg ctgttgtcct
     1501 tcatgcacct cggcatcccg ctgtttctgc tgctcgggat gtggatccat atccagcgca
     1561 tcagccgcgc caaagccagt ccgccgcgcg cgatcatgct cggtacgctg gcggcgctga
     1621 tagtgctgtc gctcgtgtat ccggcgctga gccagggacc ggccgatctc gacacggtcg
     1681 tgagcatggt gcggcccgac tggttttatc tcatgctcta tccgctgatt gatgcctggt
     1741 cgccgggcgc cgtctggatc gtggcgggcg gcggcacctt gttgcggatg ttgttgccgt
     1801 ggctgccgcg ccggccggag gaggccgcgg ccgtggtcag tctgccgaat tgcaacggct
     1861 gcggacgctg cttcgcggac tgtccctaca gtgccgtgat catgcagcca cgcaccgacg
     1921 ggctgcctta tgacagccag gcttccgtga atccggattt gtgcacggcc tgtggaatct
     1981 gcgccggcgc ctgtccgacg gcgacgccat tccggcgcac gacggagctt gttgcgggca
     2041 tcgacatgcc gctcctgcca gtgcgcgtga tacgccaacg cacgctggat gccatggcgc
     2101 gactttccgg aaacgaccgg gtgctcatat acggctgtcg ttccggtggc gacctgacct
     2161 cgcttgaaag atcgggcgtc ggggtggtca ccttgccttg cgtcggggcc ttgccgcctt
     2221 ccttcatcga ttttgccatc agtcgccgcc acgccgatgg cgttatcctt gtcggttgcg
     2281 cgcagggcga ctgttaccag cgtttgggca tagactggac ggaacaacgc ctggcgggac
     2341 aacgcgatcc atacctgcgc ggccgggtgc cgcgcaatcg cgtggccacg atctgggcag
     2401 gaccgcggga cggcgcggaa ctggcgcgcc gggttgcggc gttccgggcg cagttgcggg
     2461 ctatggggcc tttcccgcaa ggcggaacac catccgattc atcttctcag agcgagatgc
     2521 aaaatgtctc ttatcgaatg ggatgagaaa ttttccgtcg gcgtcgcggc ggtggatcac
     2581 gaacatcgcg agctgatcgg gctcgtgaat gaaacctacg acaaactcaa gcgccccgac
     2641 gccggcatcg ccgtgccgga ttttctcggc gagatttatg cgcgtatatc ggcgcacttc
     2701 gcgctcgagg aaaagctcat gcgcgcgaac cgttacgcgc atctcaacga gcacaagtca
     2761 gaccatgagc gcctgctgga cgaaattcgc aatatcatgg acgattacga agacgggcgg
     2821 gttttcgaca cggagcaatt cgcgcagcgc ctgggtgcgt ggttcaccga acatttccgt
     2881 acccacgacg cgcgcctgca ccggcagttg gattaagaga gttttgattt actatgatca
     2941 tctgccgcaa aggcgcaaag acgcaaagat ttctcgggtt ttattctcct gagcttttct
     3001 ttgcgccttc gcgtctttgc ggcaagaatt cataaaaggg tttttatgct gaaaaacatt
     3061 ttgcggtatt ccggtcaagc agtgttttac ggcctgttcg tggcctttat cggttacttt
     3121 tcgacggccc cggcctacgt gcatttcccg tccgatcagg cgctgatcaa ggtaagcttc
     3181 agtcacgccg gccagccgct ggaggaatgc cgcgtgcgta ccgccgagga actggcgaaa
     3241 ctgccgccca acatgcgcgt gcctacgcag tgcgggcgcg agcgttcccc cctgacgttc
     3301 gaactggaac tggacggcaa gccggtttat cgggcggaat tgccgccccg gggcctgtcg
     3361 cgtgacggtg cttccaccgt gtatcagcgc ttcccggtgc ccgcggggca gcatcgtttc
     3421 cgcgcacgtt tgaaagatag cgtgcacgtg tcggacttta actacacgaa ggaggcggag
     3481 gtgactctcg cgccggggca ggtgttcgtg gtggatttca atgcccgcgc tggtggattt
     3541 gtatttaaat aaaagacgag aattgcgaac catgtgtgtc catccgtggt tccaaaaatc
     3601 ataatgaatc cttccgattt ctcgcttccc gtgcccgacg atgcgcgtcg taacctggcg
     3661 cgcggctggc tgctgctggg actggcctcg ctgctggtct cgggcgtttt ctcggtgttg
     3721 ctggtgctgg cgcgcgcccc ctacgtgcag aattttttcc cgtgggtgga ttttttccac
     3781 acggcgctgg tggtgcacgt ggacctctcg gtgctggtgt ggtttctggc ctttggcggc
     3841 gtgctgtgga gccttaattc cacgccgcgg tttttgggcg ccggccggct ggcgctgctg
     3901 ctcgctttac tgggaacgct ggtgatggcg actgcgccct tcctcggcgc aggcgaggca
     3961 ctgatgagca actatattcc cgtgttgcag catccgttgt ttctggccgg tttactgctg
     4021 tttgccggtg gcaccggcgt gctggtactg cgcgccatgg gcgcgattcc gccggtgggc
     4081 cagtggatgc agggcgcggc ggcactgcgt ttcgggctca acacggcggc ggtgtcggcc
     4141 atgctcgcgc tcatggcttt tgcctggtcg tatttcgcgg ttccggattt cctgcgcggc
     4201 aagccgtatt acgaactgct gttctggggc ggcgggcatg ttctgcaatt cacctacacg
     4261 ctgctcatgc tggtgtcatg gctgtggctg gcctcggcca gcaatgtgcg tctcgccctg
     4321 agcccgcgcg tggcgctgat cttttttgga ttcggtctga ttgccgtttt ctttacgccc
     4381 atcatttatt actcctatac ggtaacgtca gtggagcaca tcaagctgtt tacctggctg
     4441 atgcaatatg gcggcagtct cgccacgctg ccattgagtc tggcgctgct gcatggactg
     4501 tggcgggcgg agcgcgcgca gggagacgcg caaccgctgc gcgccgcgct gatcgcctcg
     4561 atgatcctgt ttggcgccgg cggcgtgatc gggtttctca ttaccggcac caacgtcacc
     4621 attccggcgc attatcacgg cagcatcgtc ggtgtgaccc tgtcgctcat ggggctgact
     4681 tattatctcc tgccgcattt cggataccgc cggccggaca tgaaactggg tcgtcttcag
     4741 ccttaccttt acggcggtgg ccagttgctg cacgtgctcg ggttggtgat atcgggcggt
     4801 tacggcgtgc agcgcaaggt cgcgggggcg gaacaggcgc tcgattccgc tggccgcatc
     4861 gccggcatgg ggctgatggg actgggcgga ctgatttcca tcgccggcgg gtttttgttc
     4921 atctacgttt gttaccgcgc catgtggcct aagcgatcgc tcagcgcaaa accctgagcc
     4981 gcttcacggt aattccttcg ag
//
LOCUS       DBNGZE010000269         9030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_316542, whole genome shotgun sequence.
ACCESSION   DBNGZE010000269 DBNGZE010000000
VERSION     DBNGZE010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_316542"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC16FV_06965"
     CDS             <1..210
                     /locus_tag="AC16FV_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR2849832.1"
                     /translation="RRRWLIEGVFDRELAFLIVPNGIKGVRFIQREHFSGFLAPLIMH
                     FISEKTLKAFNLMNLALKRTAETKH"
     gene            210..2528
                     /gene="parC"
                     /locus_tag="AC16FV_06970"
     CDS             210..2528
                     /gene="parC"
                     /locus_tag="AC16FV_06970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018168104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR2849833.1"
                     /translation="MAKKTTPGENGAKKKTPSKRGKVQPVARPRAASIERLPLAAFTE
                     KAYLDYSMYVILDRALPHLGDGLKPVQRRIIYAMSELGLNAASKHKKSARTVGDVLGK
                     FHPHGDTACYEAMVLMAQPFSYRYPLIDGQGNWGSPDDPKSFAAMRYTESRLTRFAQI
                     LLSELEQGTVDWTPNFDGTLEEPKLLPARLPHVLLNGTTGIAVGMATDIPPHNVREVA
                     AACVHLLEEPDAGIKDLCKHIKGPDYPTEAEVITPKNEIRTIYETGNGSIRARAKWES
                     EDGNIVITALPYQVSGSRVIEQIAQQMQQKKLPMVEDLRDESDHENPTRIVIVPRSNR
                     VDPVELMSHLFATTDLERSYRVNLNMIGLAGRPRVYNLKELLKEWLSFRTETVRRRLQ
                     HRFERVEARLHILDGLLVAFLNLDEVIRIIRNEEEPKPRLMKRFKLTETQAEAILETK
                     LRHLAKLEEMKIRGEQDELTRERNHLEKTLKSARLLKQLMRDEIIEDAKEFGDARRSP
                     LVEREAAQQIDQTALLPTEPVTVILSEKGWVRAAKGHDMDPRTLDYKAGDKFHQAARG
                     KTNQLAVFIDSTGRTYSLPAHKLPSARGHGEPLSSSLNPPPGATWAGVMMGAPKDLYL
                     LATNAGYGFVAKLEDLISNKKAGKTVLKATKDAKVLPPQPVTDINKDMLVAATTTGQM
                     LVFPVKDLPQMPKGKGNKILNVPTAKFAKGEEAIAGVAVFTEGKSFVVHTGKGDLTVS
                     REEVELYEGERAQRGSRLPKGYQEVQGIEVKE"
     gene            2595..2963
                     /locus_tag="AC16FV_06975"
     CDS             2595..2963
                     /locus_tag="AC16FV_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849834.1"
                     /translation="MNQPTLIFVYNADSGFVNTLLDIGHKIVSPQTYACNLCAITHST
                     FSMRDEWKNFVAGLGVPIEFLHRDELEKQYGMRHVGLPAVFRRTDGTLETWVSREEIN
                     NCRSLEDLERLIRAKLGSRE"
     gene            3091..3435
                     /locus_tag="AC16FV_06980"
     CDS             3091..3435
                     /locus_tag="AC16FV_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR2849835.1"
                     /translation="MDVMVNGRKIETDEQGFLLHGDDWSEGFVERVAEQDGVRLYDES
                     WGLIGYFHEYYQQYSTHPSMRQLVLTLGRQHGMHFHDRKAYEKFLYRLFPRDPMRELC
                     KLAGLPKPEPDT"
     gene            3682..4356
                     /locus_tag="AC16FV_06985"
     CDS             3682..4356
                     /locus_tag="AC16FV_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HIR2849836.1"
                     /translation="MLDIPTADILSLIWFIAIWVGYTWYADRGAWRKHSLRAVMHAHR
                     EEWMRQMVLRDNRVADVNILRNLLQGVSFFASTTLLILAGLMAILGASDRGIEIVRGL
                     PFAARTTLGQWELKLLVLGVIFVYAFFKFTWALRQFNYCSVLIGAAPKSTDGDYARRA
                     AEVSTHASKDFNQGLRAYYFSLAALGWFVHPWVFVAATTLVTVVLYLREHHSATMKML
                     LPGGMD"
     gene            4416..5195
                     /gene="truA"
                     /locus_tag="AC16FV_06990"
     CDS             4416..5195
                     /gene="truA"
                     /locus_tag="AC16FV_06990"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR2849837.1"
                     /translation="MRIAAILEYDGSNFSGWQRQEQARSVQATVEEALSKVADEPIHV
                     TVAGRTDAGVHALAQVLHFDTSAARSEYSWVRGANSNLPPDVALLWAGPVDDGFHARY
                     SATGRHYHYVILNRPIRPTYLAQRVTHEYRLLDVVSMQAAAKYLIGEHDFTSFRAVEC
                     QAKSPVRDLRGLEVKRHGEFVHIHAHANAFLHHMVRNLAGVLITIGAGEREPDWAKEV
                     LEARDRRAGGVTAAPHGLYLREIEYPEPFKLPRLSRDVGLW"
     gene            5220..5837
                     /locus_tag="AC16FV_06995"
     CDS             5220..5837
                     /locus_tag="AC16FV_06995"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR2849838.1"
                     /translation="MRTRVKICGITRIEDATAAARAGADAIGLVFEPKSPRYIKPDQA
                     LAIVRALPPFVTVVGLFVNAAPDTVETILGRVPLDLLQFHGDETPEQCRRYHRPYIKA
                     IHMRSDVNLHDQARRYADAEGLLLDTATANVAGGSGQTFDWSLVPTDLGKPVILAGGL
                     TPENVAEAVQKVRPFAVDVSSGVEQSKGIKDTQKISAFIEAVRNA"
     gene            5834..7033
                     /gene="trpB"
                     /locus_tag="AC16FV_07000"
     CDS             5834..7033
                     /gene="trpB"
                     /locus_tag="AC16FV_07000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR2849839.1"
                     /translation="MSAYDLPDKRGHFGAYGGMFVSETLMQPLKELREAYERLRDDKH
                     FQKEFEDDLRHYVGRPSPLYLARRLTENWGGAKIYLKREDLNHTGAHKINNTIGQALL
                     ARHMGKKRVIAETGAGQHGVATATVAARFGMQCVVYMGSEDMKRQAINVYRMKLLGAE
                     VVPVESGSKTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMLVRDFQSVIGRETR
                     TQIKELEKRLPDALVACVGGGSNAMGLFYPFIKDKGVAMYGVEAAGEGVATGHHAATL
                     CAGKPGVLHGNRTYLLETEDGQITETHSISAGLDYPGVGPEHAYLKDSGRAQYVSITD
                     KEALESFHELTRTEGIMPALESSHALAYAKKLAPELGKDKIIVVNLSGRGDKDIHTVA
                     NLEGIKF"
     gene            7161..8003
                     /gene="trpA"
                     /locus_tag="AC16FV_07005"
     CDS             7161..8003
                     /gene="trpA"
                     /locus_tag="AC16FV_07005"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR2849840.1"
                     /translation="MSRIAQKFEALKQEGRRALIPYIAAGDPEPWVTVPLMHALVKSG
                     ADILELGVPFSDPMADGPVIQRAAERALKHGISLTRVLAMVREFRDKDKTTPVVLMGY
                     LNPIEVMGYARFATEAADAGVDGVLTVDLPPEEAEAFQAALKPRHLDTIFLLAPTSPA
                     ERIKLIARAASGFIYYVSLRGVTGAANLDVREVTAKLKEIRAHTKLPLGVGFGISGPE
                     AAAQVAETADAVIVGSAVVRRMEEMAANPDKILSEVPAFIARLRAAMDHVTLTPTSAT
                     GASR"
     gene            8000..8917
                     /gene="accD"
                     /locus_tag="AC16FV_07010"
     CDS             8000..8917
                     /gene="accD"
                     /locus_tag="AC16FV_07010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR2849841.1"
                     /translation="MSWFDKLVPPKIKSQAGVKKASVPEGLWKKCPACATVLYNAEIE
                     SNLDVCPKCAHHMRIGARRRLDIFLDPEPRTEIGATLFPVDPMKFKDTKKYRDRLAEA
                     QQTTGERDALVIMEGALKGMPLVAGAFEFGFMGGSMGAVVGERFVRGVNVCLEKKIPL
                     VCFSASGGARMQEALMSLMQMAKTSAALTHMSDAGIPFISVLTDPTMGGVSASFAMLG
                     DINVAEPRALVGFAGPRVIEQTVRQKLPEGFQRSEFLLDHGAIDLIVDRREMRDKLAN
                     LLAMLTRQPAFADQPSRTAETESGISESP"
BASE COUNT         1876 a         2741 c         2745 g         1668 t
ORIGIN      
        1 cgccgccggt ggttgatcga aggtgtcttc gatcgcgaac tggcattttt gatcgtgccg
       61 aacggcatca agggcgttcg cttcatccag cgcgagcact tctccggttt tctcgcaccg
      121 ctgataatgc attttatttc agagaaaaca ctcaaagcct ttaatttgat gaatctggcg
      181 ttgaaacgaa ccgcagaaac gaaacactga tggccaagaa gaccaccccc ggggaaaacg
      241 gcgcgaagaa aaaaacgccg tcgaagcgcg gcaaggtgca gccggtcgcg cgtccacgcg
      301 ccgcctcgat cgaacgcctg ccgctcgcgg cctttaccga aaaggcgtac ctggattatt
      361 ccatgtacgt catcctcgac cgcgcgctgc cgcatctcgg cgacggcctg aagccggtgc
      421 aacggcgcat catctatgcg atgtcggagc tgggcctcaa cgccgcttcc aagcacaaga
      481 aatcggcgcg caccgtgggc gacgtgctcg gcaagttcca cccgcatggc gataccgcct
      541 gttacgaggc catggtgctc atggcccagc cgttcagtta ccgctatccg ctgatcgacg
      601 ggcagggcaa ctggggttcg cccgacgatc ccaagtcgtt cgccgccatg cgctacaccg
      661 aatcgcggct cacgcgcttc gcgcagattc tgctctccga gctggagcag ggcacggtcg
      721 actggacacc caacttcgac gggacgctgg aagagcccaa actcctgccc gcgcgcctgc
      781 cgcatgtgct gctcaacggc acgaccggca tcgccgtcgg catggcgacc gacatcccgc
      841 cgcacaacgt gcgcgaggtg gctgccgcct gcgtgcacct gctggaggag ccggacgccg
      901 gcatcaagga cctgtgcaag cacatcaagg gcccggatta tcccaccgaa gccgaggtca
      961 tcacgccgaa gaatgaaatc cgcacgatct acgagactgg caacggctcg attcgggcgc
     1021 gcgccaagtg ggagagtgag gacggcaata tcgtcatcac cgcgctgccg tatcaggtgt
     1081 cgggctccag ggtgatcgag cagattgcgc agcagatgca gcagaagaag ctgcccatgg
     1141 tcgaggacct gcgtgacgaa tctgaccatg agaatccgac gcgcatcgtg atcgtgccgc
     1201 gttccaatcg cgtcgacccg gtggaattga tgtcgcacct gttcgccacc accgacctcg
     1261 agcgcagcta tcgcgtgaac ctgaacatga tcggtctggc gggccgtccg cgggtgtaca
     1321 acctgaagga attgctcaag gaatggctca gcttccgcac cgagactgtg cgccggcgcc
     1381 tgcagcaccg cttcgaacgc gtggaggcgc ggctgcatat cctcgacggc ctgctggtgg
     1441 cattcctgaa cctggacgag gtcatccgca ttatccgcaa cgaggaagag ccgaagccca
     1501 ggctcatgaa gcgtttcaag ctgaccgaga cccaggcgga ggcgatactc gaaaccaaat
     1561 tgcggcactt ggccaagctc gaggaaatga aaatccgcgg cgagcaggat gagttgacgc
     1621 gcgagcgcaa ccacctggaa aaaaccctca agtcggcacg cctgctgaaa cagctgatgc
     1681 gcgacgagat catcgaggac gccaaggagt tcggtgacgc acgccgttcg ccgctggtcg
     1741 agcgcgaggc tgcgcagcag atcgaccaga ccgcgctgtt gccgaccgag ccggtaaccg
     1801 tgatcctgtc cgagaagggt tgggtgcgcg ccgccaaggg ccatgacatg gacccgcgta
     1861 cgctcgacta caaggccggc gacaaattcc atcaggccgc gcgcggcaag accaaccagc
     1921 tcgcggtatt catcgattcc accggccgca cctattcgtt gccggcgcac aagctgccgt
     1981 cggcgcgcgg ccacggcgag cccctgagtt ccagtctcaa tccgccgccg ggcgccactt
     2041 gggccggtgt gatgatgggg gctccgaagg atttgtatct gctggcgacc aacgcgggct
     2101 atggttttgt ggcgaagctc gaagacctga tctcaaacaa gaaggccggc aagaccgtgc
     2161 tcaaggccac caaggatgcg aaagttttgc cgccgcagcc ggtcaccgac atcaacaagg
     2221 acatgctggt ggccgcgacg accaccggtc agatgctggt gttcccggtg aaagacctgc
     2281 cgcagatgcc caagggcaag ggcaacaaga tactcaatgt tcccacggcg aaattcgcca
     2341 agggcgagga agccatcgca ggcgtggctg tcttcactga aggcaaatcg tttgtggtcc
     2401 atactggcaa gggcgacctc accgtttcgc gcgaggaggt cgagctttac gagggagaac
     2461 gcgcccagcg tggttcgcga ttgcccaagg gatatcagga agtgcaggga atagaagtga
     2521 aggagtagca aaaagccgcg aaagcggctt tttgctttcc gaaatttttt tgaaatgcag
     2581 gaggtggttt cgccatgaac caaccaaccc tcatcttcgt ctacaatgcc gacagcggtt
     2641 ttgtgaacac gctgctcgac atcggccaca agatcgtttc cccgcaaacc tacgcgtgta
     2701 atctctgtgc gatcacccat tccaccttca gcatgcgtga cgagtggaaa aacttcgtcg
     2761 ccgggctcgg cgtgccgatc gagttcctgc accgggatga gctcgaaaaa caatatggca
     2821 tgcgccatgt gggattgccg gcggtgtttc gcaggaccga tggcacactg gagacgtggg
     2881 ttagccggga ggaaataaac aactgccgct cgctggagga tctcgagcgg ctgataagag
     2941 ccaaactggg gtcacgggaa taacaccgtt attcgtgtca cattcgccta tacctatcaa
     3001 acgaacgtcg ggcgtaaaat aattctggaa cttgtcgtaa cgctgactgg taccttccat
     3061 gcggcggaaa tgttccgcag gaggtggccg atggatgtca tggtaaatgg ccggaaaatc
     3121 gagacggacg aacaggggtt tctgcttcat ggcgacgatt ggagcgaagg gttcgtcgaa
     3181 agagtcgcgg agcaagacgg cgtccggctc tacgacgaga gctggggttt gatcggctac
     3241 ttccacgagt actatcagca atacagcact catcccagca tgcgccaact cgtgctgacg
     3301 ctcggccggc agcatggcat gcacttccac gaccgcaagg cctacgagaa atttctgtac
     3361 cggctgttcc cacgcgatcc gatgcgcgag ctttgcaaac tggctggatt gcccaagccg
     3421 gaaccggaca cctaaggaac ctctgaacaa gtccgttcgt ggtgagcctg tcgaaccacg
     3481 aacggacgaa tcacccgtca tttacggcat ttctcgttca cccttcgaca agctcagggt
     3541 gaacgggaga cttaatcaga gactccctaa ttcaagacat cgctgtctgg cgcgcaacgg
     3601 aaatgacgcg ttcgtgtgga ttgaggcaac gcttgtgttc cggcatgata ctctctgcga
     3661 ataaaaggga ggcaaagtct cgtgctcgac atcccgaccg ccgatatcct gagtctgatc
     3721 tggttcattg ccatctgggt cggttacacc tggtacgcgg atcgcggcgc gtggcgcaag
     3781 cattcgctgc gcgcggtcat gcacgcacac cgcgaggagt ggatgcgcca gatggtgctg
     3841 cgggacaacc gtgttgctga cgtcaacatc ctgcgcaatc tgctgcaagg cgtgtcattc
     3901 ttcgcctcga ccactttgct gattctcgcc ggactgatgg ccattctcgg cgccagcgac
     3961 cgcggcatcg agatcgtgcg cgggctgccg ttcgcggcca ggaccacgct ggggcagtgg
     4021 gagctgaagc tgctcgtgtt gggcgtgatt ttcgtttatg cgtttttcaa atttacctgg
     4081 gcgctgcgcc agttcaatta ttgctcggtg ttgatcggcg ccgcgccgaa atccaccgac
     4141 ggcgattacg cgcgccgcgc cgccgaggtc agcacccatg cctcgaagga cttcaatcag
     4201 ggcttgcgcg cctattactt cagtttggcc gcgctcggct ggttcgtgca cccatgggtg
     4261 tttgtggccg cgaccacgct cgtgaccgtg gtgctgtacc tgcgcgagca tcattcggcg
     4321 accatgaaaa tgctgctgcc cggtggcatg gactgaatgc gtcgacggtt ttaattttca
     4381 ctccggcaag aatcagcagg tagaatacgc cccttatgcg tatcgctgcg atccttgaat
     4441 acgacggctc gaacttttcc ggctggcaac ggcaggagca ggcgcgctcg gtgcaggcca
     4501 ccgtcgaaga ggccttgtcc aaggtggcgg acgagccgat tcatgtcacg gtcgccggac
     4561 gcactgacgc cggcgtgcac gcgctggcac aggtgctcca tttcgatacc agcgccgcgc
     4621 gcagcgagta ttcctgggtg cgcggcgcca acagcaatct gccgccggat gtggcgctac
     4681 tgtgggccgg gccggtggac gacggtttcc acgcgcgcta ttccgccacc ggccggcatt
     4741 atcattacgt cattttgaat cgtccgatcc ggccaaccta tctcgcgcaa cgcgtgacgc
     4801 atgaatatcg cctgctcgat gttgtgtcca tgcaggctgc ggcgaaatat ctgatcggcg
     4861 agcacgactt cacctcgttt cgcgccgtcg agtgtcaggc gaaaagtccg gtgcgcgatc
     4921 tgcgcgggct tgaggtgaaa cgccacggcg agttcgtgca catccacgcc cacgccaatg
     4981 ccttcctgca tcacatggtg cgcaacctcg cgggcgtgct cataaccatc ggtgccggcg
     5041 agcgcgagcc ggactgggcc aaggaagtgt tggaagcgcg cgaccgccgg gccggcggcg
     5101 tgaccgccgc gccccatgga ctgtatctgc gggaaatcga atatcccgag ccgttcaaac
     5161 ttccgcgcct ttcgcgcgat gtcgggcttt ggtaagcttc gcggcctttc gagggcacca
     5221 tgcgcacgcg cgtgaaaatc tgcggcatca cccgcatcga ggatgccacg gccgcggcgc
     5281 gcgccggggc cgacgccatc ggcctcgtgt tcgagcccaa gagtccgcgt tacataaaac
     5341 cggatcaggc gctggcgatt gtgcgtgcgt tgccgccgtt cgtcactgtg gtgggactgt
     5401 tcgtgaatgc cgcgcccgac accgtggaaa ccatcctcgg tcgcgtgccg ctcgatctgt
     5461 tacaattcca cggcgacgaa acgcccgaac aatgccgtcg ctatcaccgg ccctatatca
     5521 aagccattca catgcgatcc gacgttaatc tgcacgacca ggcgcgccgt tacgccgacg
     5581 ccgagggcct gctgctcgac acggctacgg cgaacgtcgc cggcggttcc ggccagactt
     5641 tcgactggag cttggttccg accgatctgg gcaagccggt gattctggcc ggcggcctta
     5701 cgccagaaaa tgtcgccgag gccgtgcaga aagtgcgccc ctttgccgtg gatgtttcga
     5761 gcggtgtcga acagagtaaa ggaatcaagg atacgcagaa gatttccgcc ttcattgagg
     5821 ccgtgagaaa cgcatgagcg cctacgatct acccgacaaa cgcggccact tcggcgctta
     5881 cggcggcatg ttcgtttccg aaacgctgat gcagccgctc aaggaattgc gcgaggccta
     5941 cgagcgcctg cgcgatgaca agcactttca gaaggaattt gaagacgatc tgcgccatta
     6001 cgtcggccgt cccagcccgc tttatctcgc caggcgcctg accgaaaact ggggcggagc
     6061 gaagatttac ctcaagcgcg aagacctgaa ccacaccggt gcgcacaaga ttaacaacac
     6121 catcggccag gcgctgctcg cgcgccacat gggcaagaaa cgtgtcattg ccgagaccgg
     6181 cgccggccag catggcgtgg cgaccgcgac ggtcgccgcg cgtttcggca tgcagtgcgt
     6241 ggtgtacatg ggttccgagg acatgaagcg ccaggcgatc aacgtgtatc gcatgaagct
     6301 cctgggcgcc gaggtggtgc cggtcgagtc cggttcgaaa acgcttaagg acgcgctcaa
     6361 cgaagccatg cgcgactggg tgaccaacgt cgagaacacc ttttacatca tcggcaccgt
     6421 ggccggcccg cacccctatc cgatgctggt gcgcgatttc cagagcgtga tcgggcgcga
     6481 gacgcgcacc cagatcaagg aactggaaaa acgtctgccg gacgcgctcg tcgcctgtgt
     6541 cggtggcggt tccaacgcca tgggcctgtt ttatcctttc atcaaggaca agggtgttgc
     6601 catgtatggc gtggaggcgg cgggcgaggg tgtcgcgacc gggcaccatg ccgcgacgct
     6661 gtgcgccggc aagcccggcg tgttgcacgg caaccgcact tatttattgg aaaccgagga
     6721 cggtcagatc accgagaccc attccatctc cgccgggctc gattatccag gcgtcggtcc
     6781 ggagcacgcc tacctcaagg attcgggtcg cgcgcaatat gtttccatta ccgacaagga
     6841 agcgctcgaa tccttccacg aactgacccg caccgagggc atcatgccgg cgctggaatc
     6901 cagccacgcg ctggcctacg ccaaaaaact cgcgccggaa ctcgggaaag acaagatcat
     6961 cgtggtcaat ctctccggcc gtggcgacaa ggacattcac acggtcgcca atctcgaagg
     7021 aatcaaattt taataaatat ttatgattat ccaccgcaaa gacgcgaagt acgcaaagaa
     7081 aatctcatga gaataaaacc acagaaatct ttgcgatctt tgcacctttg cggtgaaaga
     7141 tattattcag gatcttttta atgtcgcgca tcgcgcagaa attcgaggcg ttaaaacagg
     7201 aaggccgccg cgccctcata ccttatatag cggcgggcga ccccgagccg tgggtgacgg
     7261 tgccgctgat gcacgcactg gtcaaaagcg gcgcggatat tctggagctg ggcgtgccgt
     7321 tttccgaccc aatggccgat ggcccggtga tccagcgggc cgccgagcgc gcgctgaaac
     7381 atggcatcag tctcacacgc gtgctcgcga tggtgcgcga attccgcgac aaggacaaaa
     7441 ccacgccggt ggtgctcatg ggctatctga atcccatcga ggtgatgggc tatgcacgct
     7501 tcgccacgga agccgccgat gccggggtgg acggcgtgct caccgtggac ctgccgccgg
     7561 aagaggcgga ggcgtttcaa gctgcactga agccgcggca tctggacacc atttttctgc
     7621 tggcgccgac cagcccggcc gagcgcatca agctcatcgc cagggccgcg agcggattca
     7681 tctattatgt ctcgctgcgc ggcgtgaccg gcgccgccaa cctggacgtg cgcgaggtga
     7741 cggccaagct caaggaaatc cgcgcccata ctaaactgcc gctcggcgtt ggcttcggca
     7801 tcagcggtcc cgaggcggcg gcgcaggttg cggaaactgc tgacgccgtg atcgtcggca
     7861 gcgccgtggt ccggcgcatg gaggaaatgg cggcgaatcc ggataaaatc ctgagcgagg
     7921 tgccggcctt catcgcccgc ttgcgcgcgg ccatggacca tgtcaccttg accccgacat
     7981 ccgccaccgg agcaagccga tgagctggtt cgacaagctg gtcccgccca agatcaagag
     8041 tcaggccggc gtcaaaaagg cctcggtgcc tgagggcctg tggaagaagt gccccgcctg
     8101 cgccacggtg ctgtacaacg ccgagatcga aagcaatctc gacgtctgcc ccaagtgcgc
     8161 gcatcacatg cgcatcggcg cgcgccgccg gctcgatatt tttctcgacc cggaaccgcg
     8221 cacggaaatc ggcgcgaccc tgttcccggt cgatcccatg aagtttaagg acaccaagaa
     8281 ataccgcgac cgcctggccg aggcgcagca gaccaccggt gagcgcgacg cgctggtgat
     8341 catggagggc gcgctcaaag gcatgccgct ggtggcgggg gcctttgagt tcggcttcat
     8401 gggcggctcc atgggcgcgg tggtgggcga gcgcttcgtg cgcggcgtga atgtttgtct
     8461 cgaaaagaaa atcccactgg tgtgtttctc cgccagcggc ggggcgcgca tgcaggaggc
     8521 gctgatgtcg ctgatgcaga tggccaagac cagtgccgcc ctcacgcaca tgagcgacgc
     8581 cggcattccg ttcatttcgg tgctgaccga cccgaccatg ggcggcgtat cggcgagttt
     8641 tgccatgctg ggcgatatca acgtggccga gccgcgcgcg ctcgtcggct tcgccggccc
     8701 gcgcgtgatc gaacaaaccg tgcgccagaa actgcccgag ggtttccaac gctcggaatt
     8761 cctgctggat cacggcgcca ttgacctgat cgtcgaccgg cgcgagatgc gcgacaagct
     8821 tgccaacctg ctggcgatgc tcacacgcca gcccgcgttc gctgaccagc catcccggac
     8881 cgcggaaacc gaatccggga tttccgaatc cccctgagat ctagaaaacc cctatccgca
     8941 gatgaacgca gatataaaag agataaacgc ggataaatca tgtgagtttt ttcttatcag
     9001 cgtacatctt ataaattgtc cgcgttaatc
//
LOCUS       DBNGZE010000270         2093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1149516, whole genome shotgun sequence.
ACCESSION   DBNGZE010000270 DBNGZE010000000
VERSION     DBNGZE010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1149516"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="AC16FV_07015"
     CDS             <1..206
                     /locus_tag="AC16FV_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2849842.1"
                     /translation="AETRLVELKRASDRIAQVIADEAKAMPAELLVMGTHGRRGLNHL
                     LLGSVAEGVMRLTPCPVLLVRSG"
     gene            complement(228..959)
                     /locus_tag="AC16FV_07020"
     CDS             complement(228..959)
                     /locus_tag="AC16FV_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR2849843.1"
                     /translation="MFTRISHRRYLLILGILFAIEWALLAMYPYDRADWALENALVAV
                     FVLVLVATHRIFPLSRVSCTLIFLFLLLHEVGAHYTYAKVPYDEAFTYFFGFSLNEAL
                     GFGRNHFDRAIHFSYGLLLAYPVREVFIRIATVRGFWAYFLPLDITMSTSMLFELFEW
                     GAAEFFGGDLGMAYLGTQGDVWDAHKDMALASLGALIAMLVTAAINLSLQRDFAAEWS
                     DSLRVKIFRPLGENAIKMMLRKNRR"
     gene            1102..1800
                     /locus_tag="AC16FV_07025"
     CDS             1102..1800
                     /locus_tag="AC16FV_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR2849844.1"
                     /translation="MPRFIRLIVTAFVLALPIAGPAAAADDTPRYNQIHFQVERSRPV
                     DNDRMHAVLSLTAEDENAARLADQINRTMDGALKTAKARPKIEVRTSSYRTWPVYDKN
                     KIRRWRATQELLLEGSDFAELGNLIGQLQERLQVNSIDFSVSPPRRATVEDELIAQAL
                     DAFKQRAELVRKQLAAKGYRLVDVSINTGGGQPVPLMRGMAMEAMEKSVAPPALEAGT
                     SVLNVSVGGVIELQ"
     gene            1878..>2093
                     /locus_tag="AC16FV_07030"
     CDS             1878..>2093
                     /locus_tag="AC16FV_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR2849845.1"
                     /translation="MPHSVTLITTLATGLGLALIMGFIAIRLKLPALVGYLIAGIIIG
                     PGTPGFVADVELSQQLAEIGVILLMFGV"
BASE COUNT          432 a          682 c          624 g          355 t
ORIGIN      
        1 gtgccgagac acggctggtc gaactcaagc gcgccagcga tcgcatcgcg caagtgatcg
       61 cggatgaggc caaggccatg cccgcggaac tgctggtcat gggcacgcac ggccggcgcg
      121 gcctgaacca cctgctcctc ggcagcgtgg ccgaaggcgt catgcgtctc acgccctgcc
      181 cggtgctgct ggtccgcagc ggctagccgc tacacgcgag accgcgttca gcgccggttc
      241 ttgcgcagca tcatcttgat cgcgttttcg cccagcggcc ggaagatttt cacgcgcaag
      301 ctgtcgctcc actcggccgc gaagtcccgc tgcaaactca ggttgatcgc cgcggtcacg
      361 agcatggcga tcagagcccc caggctcgcc agcgccatgt ccttgtgcgc gtcccagacg
      421 tcgccctgcg tcccgagata ggccatgccg agatcgccgc cgaaaaattc ggctgcgccc
      481 cactcgaaca gctcgaacag catggacgtg gacatggtga tgtcgagcgg caggaaatag
      541 gcccagaagc cgcgcaccgt ggcgatgcgg atgaacacct cgcggaccgg atacgccagc
      601 agcaacccgt aggaaaaatg aatcgcgcga tcgaaatgat tgcgcccgaa acccagcgcc
      661 tcgttcagac tgaaaccaaa gaaataagtg aaggcctcgt catacggcac cttggcgtag
      721 gtgtagtgcg cccctacttc atgcagcagg agaaacagga atatcagcgt gcaggaaacg
      781 cgcgacagcg ggaagatgcg atgggtcgcg accagcacca gcacgaacac tgcgaccagc
      841 gcattttcca gcgcccagtc ggcgcggtcg tagggataca tggccagcaa cgcccattcg
      901 atggcgaaca ggatgcccag tatcaacagg tagcgccggt gcgaaatgcg ggtaaacatt
      961 gaaacatggt atagctgttc ctggccggga taaatcggtt ccgattccat tcatccgttg
     1021 gcttcatccg ttgggcgcgg cctggcgcgg gccttcgggg tgttacaatc gcaccgtccc
     1081 catcgccgcg aggaatgcac catgccacgg ttcatacgcc tgatcgtcac tgctttcgtt
     1141 ctcgctctgc ctatcgccgg tccggccgcc gcggccgatg acacgccacg ctataaccag
     1201 atccattttc aggtcgagcg cagccgcccg gtcgacaacg atcgcatgca cgccgtgctg
     1261 agtctcaccg ccgaggacga aaatgccgcg cgcctcgccg accagatcaa ccgcaccatg
     1321 gacggcgcgc tcaagaccgc gaaggcccgg cccaaaatcg aggtccgcac cagcagctat
     1381 cggacttggc cggtgtatga caaaaataaa atccggcgct ggcgcgccac gcaggaactg
     1441 ctgctggagg gatcggattt cgcggaactg ggaaatctga tcggccaatt gcaggagcgc
     1501 ttgcaggtaa actccatcga cttctcggtg tcgccgccgc gccgcgcgac ggtcgaggat
     1561 gaattgatcg cacaggcgct cgatgccttc aagcagcgcg ccgagctggt gcgcaagcag
     1621 ctcgccgcca agggctaccg cctcgtcgat gtctccatca acaccggcgg cgggcaaccc
     1681 gtgccgctga tgcgcggcat ggccatggag gcgatggaaa aatcggtcgc accgccggcg
     1741 ctcgaagccg gcaccagcgt cctcaatgtc agcgtgggtg gagtgatcga actgcaataa
     1801 tcttgtatgg tcacaccgga tgaatcggga ctgaccccat tcattctgcg ccgttcaaca
     1861 aataagaggg aaaaccggtg ccccacagcg ttaccctgat taccacgctc gccaccggcc
     1921 tggggctcgc cctgatcatg ggcttcatcg ccatacgcct gaagctgcca gccctcgtcg
     1981 gctatctcat cgccggcatc attatcggtc ccggcacgcc cggcttcgtc gccgacgtcg
     2041 aattgtcgca acaactcgcc gagatcggcg tgatcctgct gatgttcggc gtc
//
LOCUS       DBNGZE010000271         5485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_871654, whole genome shotgun sequence.
ACCESSION   DBNGZE010000271 DBNGZE010000000
VERSION     DBNGZE010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_871654"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC16FV_07035"
     CDS             <1..420
                     /locus_tag="AC16FV_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HIR2849846.1"
                     /translation="ETMLRSDKYQEFFHHMYGDLPDHWNENLHGFDRLRVIINTFTRL
                     RYCDLDGNMDLRPKGPPGNQPAYLLPWFQVPGRRSHGLKIILGHWSALGLWQGDGVIG
                     LDSGCFWGRSLSAARLDVEPVEFFSVDCEPHRSPHVT"
     gene            471..857
                     /locus_tag="AC16FV_07040"
     CDS             471..857
                     /locus_tag="AC16FV_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR2849847.1"
                     /translation="MTWLADLVLAVHVAFILFVVGGQVLIIAGWILGWTWPRHWLFRL
                     LHLLAIGFVMLQAWLGVICPLTTLENFLRAPVVGGVTYESGFIRYWVRELVFYTAPGW
                     VFTLIYTVFAGFVVVTWFVYPPRRKL"
     gene            919..1302
                     /gene="apaG"
                     /locus_tag="AC16FV_07045"
     CDS             919..1302
                     /gene="apaG"
                     /locus_tag="AC16FV_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR2849848.1"
                     /translation="MTASDKHQIQIDVRTTYLPGQSEPGQNRYVFAYTITITNSGSVP
                     ARLVTRHWIITDANEQKREVHGEGVVGEQPYLLPGTSFQYTSGTILETPVGTMQGSYQ
                     MIADDGTAFDAEIPSFTLSIPRTLH"
     gene            complement(1353..3782)
                     /gene="gyrB"
                     /locus_tag="AC16FV_07050"
     CDS             complement(1353..3782)
                     /gene="gyrB"
                     /locus_tag="AC16FV_07050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR2849849.1"
                     /translation="MTQAKKGPTPTYDSHSIQVLKGLDAVRKRPGMYIGDTDDGTGLH
                     HMVFEAVDNSIDEALAGHCNRIAVTIHGDNSITVSDNGRGIPTEIIPEEGRSAAEVVM
                     TVLHAGGKFDQNSYKVSGGLHGVGISVVNALSDFLELTVRRDGKVHQQKYHLGEPQGP
                     LKVIGESSETGTDVRFRPSNKIFTDTTYSYDTLAKRLRELSFLNSGVRISLLDERTGK
                     EDVFEYKGGIAAFVGHLNRNKTPLHQKPIYISAERDGITVELCMQWNDTYQENIFCFT
                     NNIPQRDGGTHLAGLRAAMTRTLNQFIEKTGLAKKEKVEISGDDAREGLIAVLSVKVP
                     DPKFSSQTKEKLVSSEVKGVVESIVNEKLSEFLEENPADAKSIAGKIVDAARAREAAR
                     KAREMTRRKGVLDLAGLPGKLADCQERDPSKCELYLVEGDSAGGSAKQARDRKFQAVL
                     PLKGKILNVERARFDKMLSSAEVATLITALGTGIGNNDFDINKLRYHRVILMTDADVD
                     GSHIRTLLLTFFYRQMPDMIGRGHVYIAQPPLFKIKHGKQERYLKDESELQEFLLEAA
                     LTDAMLQTADGQNLAGKELASLCRAHLAVEQTIRRLARRYDAEALRHLIYMPRLTPAL
                     LKDKKQAGKIAQELQARLTRNVNHGSRYDISIGIDTRNNLHEMILARHEHGATTRTKI
                     DAEFISSGEYGAMRELGERLEKYFSAEAIIQRADKNQKVASINAMLEWLMTEAKRGQT
                     IQRYKGLGEMNPEQLWETTMDPQTRRLLKVNIEDGVAADDVFTTLMGEQVEPRREFIE
                     KNALDVSNLDI"
     gene            complement(3854..4954)
                     /gene="dnaN"
                     /locus_tag="AC16FV_07055"
     CDS             complement(3854..4954)
                     /gene="dnaN"
                     /locus_tag="AC16FV_07055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR2849850.1"
                     /translation="MQITITREDLLKPLGYVTGVVERRQTLPVLSYVLLRQQDNEMTL
                     TGTDLEIEVIAKVNKAGGGNAEMTLPARKLFDICRALPGDAEITIKKESEKSIVKSGK
                     SRFALATVPVADFPFVQASQWEQALTLKQNQLRRLLEQTHFCMAQQDVRYYLNGLLLE
                     VTDKKLRGVATDGHRMAVSETGLDKDVKGDRQVIVPRKGIQEMLRLLSDSDDQVELEF
                     GANHIRARTSEFTFTSKLIDGRYPDYNKVIPAKQSKKLNLDRDLFRETLSRVAILSSE
                     KYRGVRLSLSSKSLRFTAHNPEQEEAQEEISTDYSGEGMEIGFNVNYMIEAISALHTD
                     KVEFGLNDPNSSCTLASPDTPYPQYVIMPMRL"
     gene            complement(5173..>5485)
                     /locus_tag="AC16FV_07060"
     CDS             complement(5173..>5485)
                     /locus_tag="AC16FV_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2849851.1"
                     /translation="DLISFQDRLVTINNIQKTVAEYYKIRVSDLHYKSRSRQVARPRQ
                     VAMALAKELTEHSLPEIGDAFGGRDHTTVLHACRKVSELKSKDSRITEDYGNLMRILT
                     T"
BASE COUNT         1135 a         1490 c         1481 g         1379 t
ORIGIN      
        1 gagacaatgt tgcgcagcga caagtaccaa gaattttttc accacatgta tggcgacctg
       61 cccgatcact ggaacgaaaa tttgcacggc ttcgaccggc tgcgcgtgat catcaacacc
      121 ttcacgcgcc tgcgctactg cgacctcgac ggcaatatgg acctgcgccc gaaaggccca
      181 cccggtaatc agccggcgta tttgctgcca tggttccagg ttccgggacg ccgcagtcat
      241 ggactcaaaa ttattttagg gcattggtcc gcgctgggct tgtggcaggg cgacggcgtc
      301 atcggtctcg acagcggctg cttctggggc cgctcgcttt ccgcagcgcg gctggatgta
      361 gagccggtgg aattcttcag cgtcgactgc gagccgcatc ggtcacctca tgtcacctga
      421 gacagattaa tgtctgccta ctacgcttcg cttcgttcgc ccggtcatat atgacctggc
      481 tggccgatct ggttttggct gtgcacgtgg catttatttt gtttgtcgtt ggcggacagg
      541 ttttaatcat agccggctgg atcttgggct ggacttggcc acgtcactgg ttgtttcgct
      601 tgttgcatct gctcgccatc ggcttcgtga tgctgcaggc ctggctcggt gtcatctgcc
      661 cactgaccac gctggaaaat ttcctgcgcg cgccggtagt gggaggcgtg acatatgaaa
      721 gcggatttat tcgttactgg gtgcgcgagc ttgttttcta cacggcgccc ggctgggtat
      781 tcaccctgat ttatacggtc tttgccgggt tcgtggtggt gacctggttt gtctatcctc
      841 cccgccggaa actttagtga taccctagcg cactaacggt gaggcaaaat aatcctcgcc
      901 agcattcggg tgctacacat gaccgccagc gataaacacc agatacagat cgacgtccgc
      961 accacttatc tccctggcca atcggaaccg ggacagaatc gttacgtctt tgcctacacc
     1021 atcacaatca ccaattccgg ctcggtaccg gcgcgtctgg taacgcggca ctggataatc
     1081 accgatgcca acgaacaaaa acgcgaggtg catggcgagg gcgtggtggg tgagcagcct
     1141 tatctgttgc ctggcaccag cttccagtac accagcggca ctatcctgga aacgccagtt
     1201 ggcacgatgc agggtagtta tcagatgatc gccgatgatg gcaccgcgtt cgacgcagaa
     1261 ataccctctt ttacgctgag tatcccgcgc acgctgcact aaaggcaaag gaagttgcgg
     1321 ccagtggcca gtatcggccc agatgcggct gattaaatat ccagattgga aacgtcgagc
     1381 gcgttctttt cgatgaactc gcgccgcggc tcgacctgct cacccatcag ggtcgtgaag
     1441 acatcgtcgg cggccacgcc gtcctcaatg ttgaccttca gcagacgccg cgtttgcgga
     1501 tccatggtgg tttcccacag ctgttccggg ttcatctcac ccaggccctt gtagcgctgg
     1561 atggtctggc cgcgcttggc ctcggtcatc agccactcca gcatggcgtt gatactggcc
     1621 accttctggt ttttgtcagc acgctggatg atggcctcgg cgctgaaata tttttccagc
     1681 cgctcgccca attcgcgcat ggcgccgtac tcgccggagg aaatgaattc cgcgtcgatc
     1741 ttggtgcggg tggtggcgcc gtgctcatgc cgcgccagga tcatttcatg caggttgttg
     1801 cgggtgtcga taccgatgga aatgtcgtag cggctgccgt ggttcacatt gcgcgtcaga
     1861 cgtgcctgaa gttcctgggc gatcttgccg gcctgcttct tgtccttcag cagcgcgggt
     1921 gtgagccgtg gcatgtagat caggtgacgc agggcctcgg catcgtagcg gcgcgcaagg
     1981 cgccggatgg tctgttccac agcaaggtgt gcgcggcaga gcgaagccaa ttccttgccc
     2041 gcgagattct ggccgtccgc ggtttgcagc atggcatccg tcagcgcggc ttcgagcaga
     2101 aattcctgga gctcgctttc gtccttcaga tagcgctcct gcttgccgtg cttgattttg
     2161 aacaacggcg gctgggcgat gtacacgtgg ccacgaccga tcatgtcggg catctggcga
     2221 tagaaaaacg tcagcagcag ggtgcggata tgcgaaccgt caacatcggc atccgtcatc
     2281 aggatcacgc gatgataacg taacttattg atatcaaaat cattattccc gatcccggta
     2341 ccgagcgcgg tgatcagcgt tgcgacttcg gcggaggaca gcatcttgtc gaagcgggcg
     2401 cgctcgacat tcaggatctt gcccttgagc gggagcacgg cctgaaattt gcgatcacgg
     2461 gcctgtttgg cggagccgcc agcggagtcg ccctccacca gatatagctc gcacttcgac
     2521 ggatcgcgtt cctggcaatc cgccagcttg ccgggaagtc cggccagatc cagcacccct
     2581 ttccgtcgcg tcatctcgcg cgctttacgc gcagcttcgc gggcgcgggc agcatcaaca
     2641 atcttcccgg ctatggactt ggcatcggcc gggttttcct caagaaactc ggagagcttt
     2701 tcattaacga tggactcgac aacccctttc acttcggacg aaaccaattt ctctttcgtt
     2761 tgcgacgaga acttcggatc cgggactttc acggacagca ccgcgatcaa gccttcacga
     2821 gcgtcatcgc cgctgatctc gaccttttcc tttttagcga ggccggtttt ttcaatgaac
     2881 tggttgagcg tgcgcgtcat ggctgcgcgc aggccggcca gatgggtacc gccgtcacgc
     2941 tgtgggatgt tgttggtgaa acagaaaatg ttctcctgat aggtgtcgtt ccactgcatg
     3001 cagagctcga cggtaatgcc atcgcgctcg gctgatatgt aaatgggctt ctggtgcagt
     3061 ggggtcttgt tgcgattcag gtggccgaca aaggccgcga ttccgccctt gtattcaaaa
     3121 acatcttcct ttccggtccg ctcatcgagc aaggaaatgc gtaccccgga gttgagaaag
     3181 gacagctcac gcaggcgttt cgctagggtg tcatagctgt aagtggtgtc ggtgaatatt
     3241 ttgttactgg gtctgaagcg gacatctgtg ccggtttcgc tgctttcacc gattaccttg
     3301 agtgggcctt ggggttcgcc caagtgatat ttctgttgat gtaccttgcc atcgcggcgg
     3361 acggtcaatt ccaggaaatc cgacagggcg ttaaccacag aaatacccac accatggaga
     3421 ccgccggaga ccttatagga gttctggtcg aacttgccgc ccgcatggag gacggtcata
     3481 actacttccg cagcggaacg cccttcttcc gggattattt cagttgggat gccacgaccg
     3541 ttatctgaaa ccgttatcga gttatcccca tggatggtga cagcgatgcg attacagtgc
     3601 ccggccaagg cctcgtctat agagttatcc acagcctcaa agaccatatg gtgtagaccg
     3661 gtgccatcgt cggtgtcacc gatatacatt ccaggccgtt tgcgcacggc atccagaccc
     3721 ttcaggactt ggatactatg cgaatcgtat gtaggggtag gtcccttttt ggcttgtgtc
     3781 attgaatagc tagtatggtt ccacgtgaaa cagttaagac aatcctttgt gtttcacatg
     3841 gaacatggcg gcatcagagc cgcattggca ttataacgta ctggggataa ggtgtgtctg
     3901 gagacgccaa agtgcagctg ctgttaggat cgttgaggcc aaactcgact ttatcggtgt
     3961 gtagtgcact gatagcctcg atcatgtagt taacgttgaa accaatctcc atgccttcac
     4021 cggagtaatc cgtgctgatt tcctcttggg cctcttcctg ctccgggtta tgggcagtaa
     4081 accgcagcga cttgctactc aagctcaacc tcacgccacg gtatttctcg ctcgataaga
     4141 tagccacgcg gctcaaagtt tcgcggaaca agtcacggtc caagttaagc ttcttcgact
     4201 gctttgctgg tataaccttg ttgtagtcgg ggtaacgtcc atcaatcagc ttggacgtaa
     4261 aggtaaactc gctggtccgg gcacggatat ggttcgcacc gaactccaac tcaacttggt
     4321 catcactgtc actgagcaag cgtaacatct cttggatgcc tttgcggggg acgattactt
     4381 gcctgtcacc tttcacgtcc ttgtccaacc cggtttcgct gactgccatg cgatgtccat
     4441 ccgtggcaac accacgaagc tttttgtcag ttacttcaag cagcaggcca ttgaggtaat
     4501 agcgtacatc ctgttgcgcc atacagaagt gggtttgttc cagcagtctg cgcaattgat
     4561 tttgtttaag agtaagtgct tgctcccact gtgaggcttg aacaaacggg aaatccgcca
     4621 ctggtaccgt agccaaggcg aaacggctct tgccagattt gacaatagat ttctcactct
     4681 ctttcttgat agtgatttct gcatcaccag ggagagcacg acaaatatca aacagcttcc
     4741 gggccggtaa ggtcatttcc gcattaccac cgcctgcctt gttaactttg gcaatcactt
     4801 cgatctcaag atcagtgcca gtcaacgtca tttcgttgtc ctgttgtcgc aacaggacat
     4861 atgagagcac aggcagcgtc tggcgacgct cgacaacacc agtgacataa cccaagggtt
     4921 ttaacaaatc ctcgcgtgtt atggtgatct gcattgcttt ttagctaatt cctttttttt
     4981 aagatagtta cttgtaatta atcaaagctt tttttgtggg taaatccgtt ttgttctgcg
     5041 attctggcag atactgaagg agtaacatgt tagcaagccc tgtaaactct tgtgcatatc
     5101 gctgacagtt gtcgaatgat accggctcgc caaggttcta caaacagaat tcacagactt
     5161 atcccaaacc attcaggtgg tcaggatgcg catgagattc ccgtaatctt cggttatcct
     5221 agagtccttg gatttcaact ctgaaacctt gcgacatgcg tgtagcaccg tagtgtgatc
     5281 ccgacctcca aaagcgtcac cgatttccgg cagactatgt tcagtcaact ccttggctaa
     5341 cgccatggct acttggcgcg gacgtgccac ctgacgcgaa cggcttttgt agtgcaggtc
     5401 agaaacgcgg atcttgtaat actcagccac tgttttctgg atattgttga ttgtcaccag
     5461 acgatcttgg aatgaaataa ggtct
//
LOCUS       DBNGZE010000272         2802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_107946, whole genome shotgun sequence.
ACCESSION   DBNGZE010000272 DBNGZE010000000
VERSION     DBNGZE010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_107946"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC16FV_07065"
     CDS             <1..546
                     /locus_tag="AC16FV_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2849852.1"
                     /translation="HIEQFSSVADFAKAELSDGPTCLVLDVRMPGRSGLELQRDLAAA
                     KKQVPIIFITAHADVPMTVQAMKGGAIEFLTKPFRDQDLLDAVEAGLARDRARRESER
                     TLGVLRERFDSLSTREREIMIQVPAGRLNKQIAADIGITESTVKVHRTHMMRKMKARS
                     LPELSRMADMLKLPSGEQQRS"
     gene            827..1288
                     /locus_tag="AC16FV_07070"
     CDS             827..1288
                     /locus_tag="AC16FV_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849853.1"
                     /translation="MERPMTTDQALPTVQELRKRLALTEAQKAAEDARRVAAAEAEKK
                     ALLEQLSKPTGISVNEHMKRAAAVVERAVVNGQTEVLVTRFPHELCTDGGRAINQSER
                     GWENTLTGQPKELYGFWDRHLRPLGYRLRVQILDYPGGIPGDIGAMLCWGD"
     gene            1333..>2802
                     /locus_tag="AC16FV_07075"
     CDS             1333..>2802
                     /locus_tag="AC16FV_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HIR2849854.1"
                     /translation="MSSIQNSQSVVSASFGPLTPAVEYMVDAAQRSILYWDVMRQRGN
                     EYRAHLAETVPHVLEFAFELVMDGRGLDRPVNYGLVRIIPPKGAIIDPTRRPFVVIDP
                     RAGHGPGIGGFKADSEIGVALKAGHPCYFIGFLPDPLPGQTIEDVARAEAVFLERVIA
                     LHPQADGKPCVIGNCQAGWAVMILAAMRPDLFGPIIIAGSPLSYWAGVHGKNPMRYSG
                     GLLGGTRLTALTSDLCNGKFDGAWLVQNFENLNPANTVWTKQYNLHSKVDTEALRYLA
                     FERYWGGHVNLNAEEIQFIVDELFVGNHLAAGEMKLSDGTAIDLRNIDRPIVVFCSKG
                     DNITPPQQALGWILDLYRDVDEIRSHGQTIVYTIHESIGHLGIFVSAGVARKQYDKFS
                     SNIDLIDVLPPGLYEAVFEPRAEGTASSDLVTGEWIMRCEERTLDDIRALGGNDIADE
                     CRFATAAKVSEINLSLYRTLAQPLVRAAVPPAMGEAMRNL"
BASE COUNT          561 a          874 c          848 g          519 t
ORIGIN      
        1 catatcgagc aattttcgtc tgtcgccgat tttgctaagg ccgagctgtc ggatgggccg
       61 acttgtctag tactcgacgt gagaatgccg gggcgaagtg gcctcgagct tcagcgcgac
      121 cttgccgcgg cgaaaaagca agtgccgatc atcttcatta cagcgcacgc cgacgtcccg
      181 atgaccgtgc aagcgatgaa ggggggcgcc attgagttcc tcacaaaacc gttccgcgat
      241 caggacctgc tcgacgccgt cgaggccggc ctcgcgcgcg atcgtgcgcg gcgcgagagc
      301 gagagaacgt tgggggtgct cagggagcgc ttcgactcgc tcagcacccg cgagcgtgaa
      361 atcatgatcc aggtcccggc cggtcgcctg aacaaacaga ttgctgccga tattggcata
      421 acggaatcca ctgtgaaggt gcatcgcacc cacatgatgc gcaagatgaa agcccgatcg
      481 cttcccgagc tcagtcgaat ggcggatatg cttaagctgc cttctggaga acagcaacgg
      541 tcctgagctt ggcttgcgga tcctaggcga gcgttgcgcc agggtccgcc tgttcaacca
      601 acatcttcat cagttccctt ccgcagagta cgggtcgtcc gcaccgacct gcatcccggc
      661 ggtgggtgca gacgctgcga tatcgccgcc accttccaac atcgtctgta agcattcgct
      721 tagcgtggac aaccagaagc gaatttcgcg ctacccgcgc acaatggatt acgactccgc
      781 aaatcgtaat gctgccagac gacacgggcg agtaatacgc caatgaatgg agcgacccat
      841 gacgaccgat caggcgctgc ccaccgttca agagctgcgg aagaggctcg ctctcactga
      901 ggcgcaaaag gcggcggaag acgcgcgccg cgtcgcagcc gcggaggccg agaaaaaggc
      961 cctgctcgag cagctctcga agcccactgg catctccgtg aacgagcaca tgaaacgcgc
     1021 tgccgctgtg gtcgagcgcg cggtcgtgaa cggccagacc gaggtcctcg tcacccgctt
     1081 cccgcatgaa ctctgcacgg atggcggacg cgcgatcaat cagtcggagc gcggctggga
     1141 gaacacgctt accggacagc ccaaggagct ctacggattc tgggacaggc accttcgtcc
     1201 gctgggatat cggctgagag tgcagatact tgactacccc ggcgggatac cgggcgacat
     1261 cggcgcgatg ctgtgctggg gcgactagtc gctaagcgcg taattcacac taacgctcta
     1321 aggagcgctt ccatgtcttc gattcagaat tcgcagtccg tcgtttcggc gagctttggc
     1381 ccgcttacgc ccgccgtcga gtacatggtg gacgcggcgc agcgcagcat cctgtattgg
     1441 gacgtgatgc gccagcgcgg caacgaatac cgcgcacacc tggccgagac cgttccgcac
     1501 gtgctcgaat tcgccttcga gctcgtgatg gatggccgcg ggctcgaccg accagtcaat
     1561 tacggcctcg tgcgcatcat cccgccgaag ggcgctatca tcgatccgac gcgtcgtccc
     1621 ttcgtggtta tcgatccgcg cgctggccat ggcccgggaa ttggtggatt caaggccgac
     1681 agcgagatcg gcgttgcgct caaggcgggt catccctgct acttcatagg cttcctgccg
     1741 gacccgctgc cgggccaaac gatcgaggac gttgcgcggg ccgaggcggt ctttctcgag
     1801 cgcgtcatcg cgctacaccc ccaggccgac ggcaagccct gcgtcatcgg caactgtcaa
     1861 gccggctggg ccgtcatgat cctcgccgcc atgcggccgg atctgttcgg tccgatcatc
     1921 atcgcgggat cgccgctgtc ctactgggcg ggtgtacacg gaaagaatcc catgcgctac
     1981 tcgggtggcc tgctcggcgg cactaggctc accgcactca cctccgatct ctgcaacggg
     2041 aagttcgacg gcgcttggct ggtgcagaac ttcgaaaacc tcaatccggc gaatacggtg
     2101 tggaccaagc aatataacct ccactccaaa gtcgacaccg aggcgctgcg ctatcttgcc
     2161 ttcgagcgct attggggcgg acacgtcaat ctcaacgccg aagaaatcca gttcatcgtc
     2221 gacgaactgt tcgtcggcaa tcatctggcg gcaggcgaga tgaagctgtc cgacggcaca
     2281 gcgatcgacc tacgcaacat cgaccggccg atcgtcgtct tctgctcaaa gggcgacaat
     2341 atcacgccgc cgcagcaggc gcttggctgg atccttgatc tctaccggga tgtcgacgag
     2401 attcgctcgc acggccagac cattgtttac acgattcatg agagtatcgg gcatctcgga
     2461 atctttgttt cggctggcgt cgcgcgcaaa caatacgata agttctcaag caacatcgac
     2521 ctaatcgacg tgctgccgcc cggcctatac gaagcggtgt tcgagccgag ggccgagggg
     2581 accgcgagct ccgatctcgt gacgggagag tggatcatgc gctgcgagga gcgcacactc
     2641 gatgatatcc gcgcgctcgg cggcaatgat attgccgacg agtgccgctt tgcgacggcg
     2701 gctaaagtct cagagatcaa tctctcgctc taccgcacct tggcgcagcc gctggtacgc
     2761 gccgcggtac cgcctgcgat gggtgaggcg atgcgcaacc tg
//
LOCUS       DBNGZE010000273         1762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1427970, whole genome shotgun sequence.
ACCESSION   DBNGZE010000273 DBNGZE010000000
VERSION     DBNGZE010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1427970"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..>1762
                     /gene="argS"
                     /locus_tag="AC16FV_07080"
     CDS             89..>1762
                     /gene="argS"
                     /locus_tag="AC16FV_07080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR2849855.1"
                     /translation="MKHHIENLIASALAGLEATGALPLNHGATITIDHAKSKEHGDFA
                     CNIALVLAKHAHVKPRELAEKIVKALPASEKIVKVEIAGPGFINFFLGANAYHAVVRE
                     ILEKREAFGRGQMGAGRKVQVEFVSANPTGPLHVGHGRGAAYGATLANLLDAVGYQVH
                     REYYINDAGRQMDILATSVWLRYLELCGEKFDFPVNGYKGDYVYDIGATLHRENGDKY
                     RHATAKVFENIPSDEPQGGDKETHIDALIERTKSLLGPADYRKVFDLGLQVILDDIRQ
                     DLEEFGVTYDQWFSERALVEDGAANRAIERLKKSGHVYEKEGALWFRSTDYGDEKDRV
                     VVRENGQKTYFAHDIAYHMDKLERGYDQVIDIWGADHHGYVPRIKAALAAIGDDPERL
                     HVLLVQFAILYKGGERMQMSTRSGEFVTLRELRREVGNDAARFFYVLRKCEQHMDFDL
                     DLAKSQSNDNPVFYVQYAHARIYSVFRQMKEKGFSHDPANGNRNLARLTEPHELALIQ
                     RLARYPEVLESAAREHEPHQLAYYLRELAYDFHTYYGAHTFLVEDAALRN"
BASE COUNT          389 a          553 c          516 g          304 t
ORIGIN      
        1 gactccccct ctcccgcaaa cgcgggagag ggcaaggggt gagggctaaa cgccagacgc
       61 agacactaca aacaattttg gacatccctt gaagcaccac atcgaaaatc tgatcgcctc
      121 ggcgctcgcg ggcctcgagg ccactggcgc actgccgcta aaccacggcg cgaccatcac
      181 cattgatcac gccaagtcca aggagcacgg cgacttcgcc tgcaacattg cgctggtgct
      241 cgccaagcac gcgcacgtca agccgcgcga gctcgcggaa aaaatcgtca aggcgctgcc
      301 tgcgtccgaa aagatcgtca aggtcgaaat tgccgggccg ggcttcatca acttttttct
      361 gggcgccaat gcgtaccacg ccgtggtacg cgaaattctg gaaaaacgcg aggccttcgg
      421 ccgcggccaa atgggtgccg ggcgcaaggt gcaggtggaa ttcgtctcag ccaatcccac
      481 tggcccgctg catgtcggcc acgggcgtgg cgcggcctat ggcgcgacgc ttgccaacct
      541 gctcgatgcc gtgggctatc aggtgcaccg cgagtactat atcaatgacg ccggccggca
      601 gatggacatc ctcgccacca gcgtatggct gcgttacctc gaactgtgcg gcgaaaaatt
      661 cgactttccc gtcaatggct acaagggcga ctacgtctac gacatcggcg cgactctgca
      721 ccgagagaat ggcgacaagt atcgtcatgc cactgccaag gtatttgaaa acattccatc
      781 cgacgagccc caaggcggcg acaaggaaac acatatcgac gcgctgattg agcgcaccaa
      841 atccctgctt gggccagcgg actaccgcaa ggtcttcgat ctcgggcttc aggtcatcct
      901 cgatgacatc cgtcaggacc tcgaagagtt tggcgtcact tacgaccaat ggttctccga
      961 acgcgcgctc gtcgaagatg gcgccgccaa tcgcgccatc gaacgcctga agaagtcagg
     1021 ccatgtgtat gaaaaagaag gggcgctgtg gttccgctcc accgattatg gcgatgagaa
     1081 ggaccgcgtg gtggtgcgcg agaacggcca gaagacctat ttcgcccatg acatcgccta
     1141 ccacatggac aagctcgaac gtggctacga ccaggtgatc gacatctggg gcgcggacca
     1201 tcacggttac gtgccacgca tcaaggcggc gctcgcggcc atcggcgacg atccggaacg
     1261 gctgcacgtg ctgctggtgc agttcgccat cctctacaag ggcggcgaac gcatgcagat
     1321 gtccacccgc agcggagagt tcgtcacctt gcgcgagctg cggcgcgagg tcggcaacga
     1381 cgccgcgcgc ttcttctacg tgctgcgcaa gtgcgagcag cacatggact tcgatctcga
     1441 tctggcgaag agccagtcta acgacaatcc ggtgttttac gtgcaatacg cgcacgcgcg
     1501 tatttacagc gtgttccgcc agatgaagga aaaaggcttc agccacgacc cggcgaacgg
     1561 aaaccggaac ctggcgcgcc tgacggaacc gcacgagctg gcgctgatcc agcggctggc
     1621 gcgctacccg gaggtactgg aatccgcggc gcgtgaacac gaaccgcacc agcttgccta
     1681 ttatctgcgc gaactcgcct acgactttca cacctattac ggcgcccaca cctttctggt
     1741 ggaggatgcc gcgttgcgca ac
//
LOCUS       DBNGZE010000274         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_38772, whole genome shotgun sequence.
ACCESSION   DBNGZE010000274 DBNGZE010000000
VERSION     DBNGZE010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_38772"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="AC16FV_07085"
     CDS             <1..1557
                     /locus_tag="AC16FV_07085"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR2849856.1"
                     /translation="TDAITHFERACHLNPEDAELWMRLSEASWWEGKYSDALRAVERA
                     VKLRPDWAEAYNVLGRAQLETGTGVIERNRAAEQSFRKAVELKPDFTEAHIHLAASLR
                     SQGRYAEALESYRRVMTLSPRRPDAVGGASSMLERLGRFDEARELLATMADEGITTLD
                     LALPYAAISRYMGEKNEAMELLERLLANDDHNRELRTEAHFMLGKLYDSEKDYDRAFR
                     HYRAANDLDLDKFDIDSNGRTFERLIGYFAKETHHRRPRASNRSRLPVFIVGMPRSGT
                     TLTEQILASHPLVHGAGELPRMDEIKKTLHELMGEPWPQCVDRLDRKTLDKVAQEHLD
                     WLGRLAQGKARVTDKMPHNFLALGLIDLLFPEAQVIHCRRDPMDNCLSIYFQHFNGAH
                     AYANNLEHLGIYYRQYERVMAHWRQVVRVPILDVRYEQMVEEPEQSARRLVEFCGLDW
                     DDRCLRFYETERVVSTPSYDQVRQPIYKKSVARWKRYEKFLEPLMRSLGREETSAQPV
                     ADGSRRSTTT"
     gene            complement(1515..>2655)
                     /locus_tag="AC16FV_07090"
     CDS             complement(1515..>2655)
                     /locus_tag="AC16FV_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2849857.1"
                     /translation="EKCYREALRLQPNFPDALTNLGLVMQDRGRLTEAVELHRHALAL
                     APQHVDAHYNIGYALILLGEAKPAIPHLERVTQLRPGDARGWISLGGAYARTHDHARA
                     VQCYERAATLDPNNFENFSTLGVSYLTAGERDKSIAALKHALELKPGDADTRYWLAAA
                     GVGETPDMMSPDGVAKLFDGYAGNFDEHLVNKLRYRAPEMLSAALRRGLGDRVTLLSV
                     ADLGCGTGLLGAELRDIADYIAGVDLAPKMIEKARARGVYDDLVLQDVTEFLEATPRQ
                     FDAVVATDVFIYIGKLDRIFAAASACLVAGGLFGFSVEAHPGEEPYHLRTSGRYAQSL
                     RYLRELSSRYGFAEVSVDPVVLRLENGVPIDGYVVVLRRLPSATG"
BASE COUNT          522 a          833 c          830 g          470 t
ORIGIN      
        1 actgacgcaa ttacccactt tgagcgggca tgtcacctga acccggaaga cgccgagcta
       61 tggatgcgtc tgtcggaggc aagctggtgg gagggcaagt attccgacgc gcttcgtgcg
      121 gtcgagcgtg cggtgaaact caggccggat tgggcagagg cgtacaacgt gctcggtcgt
      181 gcgcaactgg agaccggcac cggggtcatc gaacgcaacc gcgccgccga gcagagtttt
      241 cgcaaggcgg tcgaactcaa gccggatttc acggaggccc atatccacct ggcggccagt
      301 ttgcgctcgc agggacggta cgcggaggcc ctggagtcct accggcgcgt gatgaccctt
      361 tcgccgcgga gacctgacgc ggttggaggc gcgtcatcca tgctcgaacg tctcggccgg
      421 ttcgacgagg cgcgcgagct cttggcgacg atggccgatg aggggatcac gacactcgac
      481 cttgcgcttc cctacgccgc gatttcacgc tacatgggcg aaaagaatga agcgatggaa
      541 ttgctggaac gtctgctggc caatgatgac cataatcgcg aattgcgcac cgaggcccac
      601 ttcatgctcg gcaagctcta tgacagcgaa aaagattacg accgggcgtt ccggcattat
      661 cgcgcggcca acgatctcga tctggataaa ttcgatattg acagcaacgg ccggacattc
      721 gaaaggctca tcggctactt cgcgaaggaa acgcatcacc ggcgcccgcg cgcgtcgaac
      781 cgctcgcggc tgccggtttt catcgtcggc atgccgcgtt ccggcaccac gctgaccgag
      841 cagatcctgg ccagccatcc cctggtccat ggggccggcg agttgccgag aatggacgag
      901 atcaagaaga cgctgcacga gctcatgggc gagccctggc cccaatgcgt ggaccgcctc
      961 gaccgcaaga cgctcgacaa ggtggcgcag gagcatctgg actggctcgg ccgcctggcg
     1021 cagggcaagg cccgtgtcac cgacaagatg ccgcacaatt ttctcgcgct cgggctgatt
     1081 gacctgctgt tcccggaggc gcaggtcatc cactgccggc gcgacccgat ggacaattgc
     1141 ctgtccatct attttcagca cttcaacggc gcgcacgcgt atgccaacaa cctggagcac
     1201 ctcggcatct actaccggca gtacgagcgg gtcatggcgc actggcgcca agtcgtgcgc
     1261 gtaccgatct tggacgtgcg ttatgaacag atggtggagg agcccgagca aagcgcccgc
     1321 cggctggtgg agttctgcgg actcgactgg gacgaccgtt gcctgcgttt ttacgaaacg
     1381 gagcgggtgg tatccacgcc cagctacgat caggtccggc agccgatcta caagaagtcg
     1441 gtggcgcggt ggaagcgtta cgagaaattc ctcgagccgc tcatgcgctc gctcggccgg
     1501 gaagaaacct cggctcagcc cgttgccgat ggcagccggc gcagtaccac gacgtaaccg
     1561 tctatcggga cgccgttttc cagtcgcagc acgaccgggt caacactgac ttccgcgaag
     1621 ccgtaacgcg acgacaactc gcgcagataa cgcagagatt gggcatatcg ccccgaggtc
     1681 cggaggtggt atggctcctc gccgggatgc gcctcgaccg agaagccgaa caggccgccg
     1741 gcgacgaggc acgccgatgc cgcggcgaaa atccggtcga gtttcccgat gtagatgaac
     1801 acgtcggtgg ccaccaccgc gtcaaactgt ctcggcgtgg cttccaggaa ctccgtgaca
     1861 tcctgcaata ccaagtcatc gtaaacgccc cgggcccgcg ccttctcgat catttttggc
     1921 gccaggtcca cgccggcaat atagtccgcg atgtccctca attccgcccc gagcaatccg
     1981 gtgccgcacc cgagatccgc gacggacagc agtgtcacac gatcgccgag gccgcggcgc
     2041 agggcggcgc tgagcatttc cggagcgcgg taccgcaact tgttcaccag atgttcgtcg
     2101 aaattgcccg cgtacccatc gaagagcttc gcgacaccat ccggactcat catatcgggc
     2161 gtctcgccga caccggcggc cgcaagccag tagcgggtat cggcatcgcc cggtttcagc
     2221 tcgagcgcgt gtttgagtgc cgcgatactc ttgtcacgtt caccggcggt gagataactg
     2281 acgccgagag tcgagaaatt ctcgaaattg ttcgggtcta gagtggcggc tcgttcgtag
     2341 cattgcaccg cccttgcgtg gtcgtgcgtg cgagcgtagg cacccccgag ggaaatccat
     2401 ccccgtgcgt cgccgggccg cagttgcgta acccgctcca gatgcggaat cgcaggtttt
     2461 gcctcgccca gcagaatcaa tgcatatccg atattgtagt gggcgtccac gtgctgcggg
     2521 gcgagcgcca gcgcgtgacg atgcagttcc accgcttccg tcaggcgacc gcgatcctgc
     2581 atgacgagcc cgagattggt gagtgcgtcc gggaaattcg gctgaagacg cagggcctcc
     2641 cggtagcact tctca
//
LOCUS       DBNGZE010000275         6303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1358535, whole genome shotgun sequence.
ACCESSION   DBNGZE010000275 DBNGZE010000000
VERSION     DBNGZE010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1358535"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="bufA2"
                     /locus_tag="AC16FV_07095"
     CDS             <1..718
                     /gene="bufA2"
                     /locus_tag="AC16FV_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BufA2 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR2849858.1"
                     /translation="RSAGGQLAGIGGAMAASAACFGAPVLVSAATAIGATALTSHATL
                     YPIFVGAIVLSLWQLYRSTRERGRLAPFFLGLAGGATAAVFLWLMVTGIVPLPGVALY
                     GALGVLVAGSLWEMAGRFLEVCVTKIRREMAYEKKIVPVGRKITNGAAISIAAAAAFY
                     GMYKSVEAFAPKAEAAEIACFGINACKGQTACATAWNACPGQNSCKGKGFLNTSAKEC
                     TDKGGVPLKGSPADPARQNT"
     gene            836..1333
                     /locus_tag="AC16FV_07100"
     CDS             836..1333
                     /locus_tag="AC16FV_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group I truncated hemoglobin"
                     /protein_id="HIR2849859.1"
                     /translation="MKPTMHLLPGALLLLFAAILLLVGCATAYSPSSEKAATVPLYDR
                     LGGVYAIATVVDDFIERLLVNDTLNANPAIKTARDRVPKSGLKHQVTAFVCQATGGPC
                     TYIGRSMKQSHAHLNIGEKEWQAMASEFKKSLDKFSVPQRDQDELFALVGSVKGDIVT
                     ATAKN"
     gene            1349..2140
                     /locus_tag="AC16FV_07105"
     CDS             1349..2140
                     /locus_tag="AC16FV_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR2849860.1"
                     /translation="MSMPSVSAEKSVNRIDLEQKVKEMYQRVALEPFGEFHFEMGHAL
                     AERLGYAPADLDRISREAVDSFAGVGYYFDLAAIKTGDTILDLGSGSGMDSFIAALKT
                     GATGKVLGVDMTDAQREKAGNLRDRDGFTQVSYHKSYIENTPFDNNTIDVVISNGVIN
                     LAADKAAVFREAARVLKPGGRLAISDIITEKELPEGVTCNATLWAACIGGALQQERYL
                     DAIRDAGLRLITLRSNPQYRFLTASAQKACATYGVKSVSLLAVKS"
     gene            2342..2788
                     /locus_tag="AC16FV_07110"
     CDS             2342..2788
                     /locus_tag="AC16FV_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849861.1"
                     /translation="MRLTQILPDQVRAFYAARGFDATSVELLAARACVFQTVFRNESA
                     KGAIEFNLADWRVITVKNERPLKLERDWQKEWEKRGVSAAARTAFRYALYPTEHRYEL
                     GDWNMGMTTYALPLGSRFDLRFVWRENGSQREAMLMGVHCAKETVR"
     gene            2716..3312
                     /locus_tag="AC16FV_07115"
     CDS             2716..3312
                     /locus_tag="AC16FV_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2849862.1"
                     /translation="MARKRFATRGHVDGRALRQGNGQVKTAVAVILACLLTLQHVVRA
                     ESPPQTMSAMPDRPTAPDFVLQDADRKLYRLSEQRGKVVMVNFWATWCPPCRREMPSM
                     QRAWEALKGEKFLMFAVNVGEDEDTVFGFTFSTGVELKFPILLDRDAVVIKAWPVIAL
                     PTSFIVDPRGRIVYRAVGGREWDDPELLGKIRELLRPE"
     gene            complement(3319..4677)
                     /locus_tag="AC16FV_07120"
     CDS             complement(3319..4677)
                     /locus_tag="AC16FV_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR2849863.1"
                     /translation="MSSNITSTLQSLINTHENPVVLIDENYEVVAANGAYQAAYGMEP
                     SQIIGKYCYEISHHRDSPCWQHGEDCPHREVFQNNRPCQSLHTHYDAIGRAEHVQIKG
                     YIIPRPDGSKLLAESIFRLTSPEELDCDEMRMIGRSPAFLHAIEHLTRAADSNANVLL
                     YGESGVGKELAAHYVHQHSPRKKGPFMAVDCASITESLFESEIFGHERGSFTGCAGRK
                     RGLFELSDGGTLFLDEVGELSAATQAKLLRVLETGEFRRVGGREVLRADVRVIAATNR
                     HIRAMVERGDFREDLYYRMACINVDLPPLRERRSDIPVLAEALLNRINLANHSHCYLT
                     GEAMEKLTNYDFPGNVRELRNILHRAAAMCGAHNGAITAAGLVFDDAGSPMPGGNGGR
                     KISSPAPLSTGKQLLSMREVESRYMADLLARYEGQRRPVAEAMGISERTLYRKLKRYG
                     LN"
     gene            4883..6160
                     /locus_tag="AC16FV_07125"
     CDS             4883..6160
                     /locus_tag="AC16FV_07125"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2849864.1"
                     /translation="MAHIVILGAGIGGLPAAYELRDVLGSKHRITVVNASESFQFVPS
                     NPWLAVGWREREQITFPLREPLERRGIGFIAQTVTKIDAAQNRLALANGDTLDYDYLI
                     IATGPKLAFDEVPGAGPGGHTHSICTTEHAEKAWLDDQKLLAEPGPVVIGAMAGASCF
                     GPAYEFAFIMDADLRRRKIRHKVPITFVTSEPYIGHLGLGGVGNSQGLLEAEMRDHHL
                     KWIVNAKVNRVEPGKMFVAQYNDKGEVAKEMEVPFKFSMMLPAFKGVDAVAAVEGLCN
                     PRGFVLVDQHQRSKKYRNIFSAGVCIAIPPVEVTPVPTGAPKTGYMIETMVTAIVRNI
                     KAEVDGYAPLYSGTWNAFCLADMGDKGIAFVAIPQIPPRNVSWMKKGRWVHWSKIAFE
                     KYFIRKMKKGKSEPYYEKYILRLLGIHRLDKAA"
BASE COUNT         1278 a         1924 c         1897 g         1204 t
ORIGIN      
        1 acgcagcgcg ggcggccagc tcgccggcat cgggggcgca atggcggcga gcgccgcctg
       61 ttttggcgcg ccggtgctgg tgtcggcagc cactgcgatc ggtgccacgg ccttgacctc
      121 gcacgcgaca ctctatccga tcttcgtcgg cgccatcgtg ctgagtctgt ggcagcttta
      181 ccgctccacg cgcgagcgcg gcaggcttgc gccgtttttt ctcggactgg cgggcggcgc
      241 caccgcggcg gtgttcctgt ggctcatggt cacgggcatc gtgccgctgc cgggcgtcgc
      301 gctctacggc gcgctcggcg tgctcgtcgc cggcagcctg tgggagatgg ccggacgttt
      361 cctggaggtg tgcgtcacga aaatccggcg tgaaatggcc tacgagaaga agatcgtccc
      421 ggtcggcagg aaaatcacca acggggccgc gatttcgatc gccgccgcgg cagcatttta
      481 cggcatgtac aagtcggtgg aggcgttcgc gccgaaggcc gaggcggcgg aaattgcctg
      541 cttcggcatc aacgcctgca agggccagac cgcctgcgcc accgcttgga acgcctgccc
      601 cggacagaat tcctgcaaag gcaaaggctt cctcaatacc tcggcgaagg aatgcaccga
      661 caagggcggc gtgccgctca aaggctcgcc cgctgatccc gcgcgtcaaa acacctgacg
      721 cgctgccccc tcgtaccgat tctgtctccc aaaatgcggg aactatcctg tgggcggttg
      781 cgtctttgcc gatgcaacac aaacatttgc actcgatgca tcccaggagg agatcatgaa
      841 acccacaatg catttgcttc ccggcgcgct attgttgctg ttcgccgcca tactgctgct
      901 cgtcggatgt gcaaccgcgt acagcccgtc atccgagaag gccgccacgg ttccgctcta
      961 cgaccgcctc ggcggtgtat atgccatcgc cacggtggtc gacgacttca tcgagcggct
     1021 gctcgtcaac gacacgctca acgccaaccc ggcaattaaa actgcgcgcg accgcgtgcc
     1081 caagtcgggg ctcaaacatc aggtcaccgc gttcgtgtgt caggccaccg gtgggccgtg
     1141 cacgtacatt ggccgcagca tgaagcagtc gcacgcgcac ctgaatatcg gcgagaaaga
     1201 atggcaggcc atggcctccg aattcaagaa gtcgctcgac aaattcagcg tgccgcaacg
     1261 cgaccaggac gaactgttcg ccctggtcgg cagcgtcaag ggcgacatcg tcacggccac
     1321 cgccaaaaac tgactcaagg agaaaaccat gagcatgcca agcgttagcg cggaaaaatc
     1381 cgtcaaccga atcgacttgg agcaaaaggt aaaagagatg taccagcggg tggcgctcga
     1441 gcccttcggc gagttccact tcgagatggg ccacgcgctc gccgagcgtc tcgggtatgc
     1501 gcccgccgat ctcgaccgca tctcgcgcga agccgtggac tcgttcgccg gcgtaggcta
     1561 ttacttcgat ctcgctgcga tcaagaccgg cgacaccatc ctcgacctgg ggagcggctc
     1621 cggcatggac agcttcatcg ccgcactcaa gaccggcgcc actggaaaag tccttggggt
     1681 agacatgacc gatgcccagc gcgagaaagc gggaaacctg cgtgatcgcg acggtttcac
     1741 ccaggtgagt taccacaagt cctatatcga aaacacaccg tttgacaata acaccattga
     1801 cgtcgtcatc agtaatggcg tgatcaatct cgcggccgac aaggcggcgg tgtttcgcga
     1861 agcggcgcgc gtactcaaac ccggcgggcg gttggccatt tccgacatca ttacagaaaa
     1921 ggagctgccc gagggcgtta cctgtaacgc cacactctgg gccgcgtgca tcggcggtgc
     1981 cctgcagcag gaacgttacc tcgacgcgat tcgcgacgcc ggattacgat tgattacgct
     2041 gcgttcgaac ccgcaatacc gtttcctcac cgcctcggcg cagaaggcct gcgcgactta
     2101 cggcgtgaag agcgtctccc tcctcgcggt aaagtcctga atgccgcgcc tcttcgctct
     2161 gacatacacc gggtgacggg ttcatgcttt aatcaacccc gtgatactgg cgttgacgcg
     2221 aaacgggata gtctgcctac tcctctgcac ggtggccgcc gcctcggcta tggaacagac
     2281 gcacacgaca gacccggaat caggaattga gacctgggag attgccacgc gaggcgtggg
     2341 cttgcgtctt acacaaatcc tgcccgacca ggtgcgcgcg ttttatgcgg cgcgcggttt
     2401 tgatgccaca tccgtggaac ttctcgccgc ccgcgcctgc gtgttccaga ccgtcttccg
     2461 caatgaatcg gcaaaaggtg ccatcgaatt caatctcgcc gactggcgtg tcatcaccgt
     2521 caagaatgaa cgaccgctca aactcgagcg tgattggcag aaggaatggg agaagcgagg
     2581 tgtgtctgcg gcggcacgca cggcattccg ctatgccctc taccctacgg aacaccgtta
     2641 cgaactcggc gattggaaca tgggcatgac gacctacgcg ctgcctctgg gaagccgctt
     2701 cgatctgcga tttgtgtggc gcgaaaacgg ttcgcaacga gaggccatgt tgatgggcgt
     2761 gcactgcgcc aaggaaacgg tcaggtgaaa acagcggtgg ctgtaatact tgcctgcctg
     2821 ctgacgctgc agcatgtggt gcgggcagaa tcaccgccgc agaccatgtc ggcgatgccg
     2881 gatcggccga cggcgccgga tttcgtgctc caggacgccg accgcaagct atatcgactc
     2941 tcggagcagc gaggaaaggt ggtgatggtg aatttctggg ccacctggtg cccaccctgt
     3001 cgccgtgaaa tgccttcgat gcaacgcgcc tgggaagcac tcaaaggtga aaagttttta
     3061 atgtttgcag tcaatgttgg cgaagacgaa gacacggttt tcggatttac attttcgacc
     3121 ggcgtggaac tgaaattccc gatcctgctg gaccgtgacg cggtggtcat caaggcctgg
     3181 cctgtcatcg ccctgccgac aagtttcatt gttgatccgc gggggcggat cgtctatcgc
     3241 gccgtgggtg gacgtgagtg ggatgatccc gaattgctgg gaaaaatccg cgagctgtta
     3301 cgtccggaat gaatagactt aattcagtcc gtaacgtttc agcttgcgat acagcgtacg
     3361 ttcgctgatg cccatggcct cggcgacggg gcggcgctgt ccctcgtaac gcgcgagcag
     3421 gtccgccatg tagcgcgatt ccacctcgcg catgctgagc agctgtttgc cagtggacag
     3481 cggtgccggg gatgaaattt tccgcccgcc gttaccgccc ggcatgggac tgccggcatc
     3541 atcgaatacc agacccgccg cggtgatcgc gccgttgtgc gcgccacaca tggccgcagc
     3601 gcgatgcagg atgttgcgca gttcgcgcac gttaccgggg aaatcgtaat tcgtaagctt
     3661 ctccatggcc tcgccggtca ggtagcaatg cgaatggttg gccagattga tgcggttgag
     3721 cagggcctcg gccagcaccg ggatgtcgct gcggcgttcg cgcaagggcg gcagatcgac
     3781 gttgatgcag gccatgcggt aatacaggtc ttcgcggaaa tcaccgcgct ccaccatggc
     3841 gcggatatgg cggttggtgg cggcgatgac gcgcacatcg gcgcgcaaca cctcgcgccc
     3901 accgacgcgc cgaaattcgc cggtctcaag aacgcgtaac aacttcgcct gcgtggccgc
     3961 cgataattcg ccgacctcgt ccaggaacag cgtaccgccg tcggaaagct cgaacagtcc
     4021 gcgcttgcgg ccggcgcaac cggtgaagga accgcgctcg tgcccgaaga tttcgctttc
     4081 aaacaacgat tcggtgatcg aggcgcaatc caccgccatg aacgggcctt tcttgcgcgg
     4141 gctgtgctga tgcacatagt gcgccgccag ctccttgccc acgcccgatt cgccatagag
     4201 cagcacgttg gcattgctgt cggcggcgcg cgtgagatgc tcgatcgcat gcagaaaggc
     4261 cggcgagcgg ccgatcatgc gcatctcgtc gcaatccagt tcttccgggc tggtcaagcg
     4321 aaaaatggac tcggcgagaa gcttgctgcc atcgggccgc gggatgatgt agcccttgat
     4381 ctgcacgtgt tccgcccgac cgatggcgtc gtaatgggta tgcagcgact ggcacggacg
     4441 gttattctga aacacctccc gatgcgggca atcctcgccg tgctgccagc agggagaatc
     4501 ccgatggtgc gagatttcat aacaatactt tccgatgatc tggctcggct ccatgccata
     4561 ggcggcctga tacgcgccgt tggcggcgac cacctcgtag ttttcatcga tcagaacaac
     4621 cggattttca tgagtattga taagcgattg aagcgtggaa gtaatattcg acgacataca
     4681 tcctccataa atgacacaaa ctgacatttt gtcagtcgaa ttggctgata atgacgccga
     4741 tgtcatgtca ttccggcaag cttgtcaatg atgtcggtca gggaagcggt ttttccggaa
     4801 aaatataata aatggtaatc tggtacaggg attgcattca ggccctccaa attttttatt
     4861 tttatatcag gaggagcaac acatggcaca tatcgtcatt ctcggcgccg gcatcggcgg
     4921 tctgcccgcg gcgtacgaac tgcgcgacgt gttgggcagc aagcaccgga tcaccgtcgt
     4981 caacgcctcg gaatcgttcc agttcgtgcc gtccaacccg tggctcgcgg tcggttggcg
     5041 cgagcgcgag cagatcacgt tcccgttgcg cgagccgctg gaacgccgcg gtatcggctt
     5101 catcgcccag acggtgacga agatcgatgc cgcgcagaac cggctcgcgc tcgccaacgg
     5161 cgacacgctc gattacgact acctgatcat cgccaccggt ccaaagctcg cgttcgacga
     5221 agtacccggc gccggtccgg gaggacacac ccattccatc tgcaccacgg aacacgctga
     5281 aaaggcgtgg ctggatgacc agaagctgct ggcggagccg gggccggtcg tcatcggcgc
     5341 catggccggg gccagttgtt tcggcccggc gtacgaattc gcgttcatca tggacgccga
     5401 cctgcgacgc cgcaagatcc gccacaaggt gccgatcacc ttcgtcacca gcgaaccgta
     5461 catcggccat ctcgggctcg gcggcgtggg caattcgcag ggactgctcg aggccgagat
     5521 gcgcgaccat catctcaagt ggatcgtcaa cgccaaggtc aatcgcgtcg agccgggcaa
     5581 gatgttcgtc gcgcagtaca acgacaaggg cgaagtcgcg aaggaaatgg aagttccgtt
     5641 caagttttcc atgatgctgc ccgcgttcaa gggcgtggat gcggtcgcgg cggtcgaggg
     5701 cctgtgcaat ccgcgcggtt tcgtgctcgt ggaccagcac cagcgcagca agaaataccg
     5761 caacatcttc tccgccggcg tgtgcatcgc gatcccgccg gtcgaggtca cgccggtgcc
     5821 caccggcgcg cccaagaccg gttacatgat cgagaccatg gtcaccgcca tcgtgcgcaa
     5881 catcaaggcg gaggtcgatg gttacgcacc gctgtacagc ggcacctgga atgctttttg
     5941 cctcgccgac atgggcgaca agggcatcgc cttcgtcgcc atcccgcaga taccgccgcg
     6001 caacgtgagc tggatgaaaa aaggccgctg ggtgcactgg tcgaagatcg cgttcgagaa
     6061 atatttcatc cgcaaaatga aaaaaggcaa atccgaaccg tattacgaga aatacatcct
     6121 gcggctgctc ggcatccatc ggctggacaa ggccgcgtaa atccccgtaa tctcctccaa
     6181 cggattgggc gcggtgtata ccgcgccctt ttttgcatcc ctcggcacca caagcagtac
     6241 ttttccgctg cgctatcctg cttcgcggaa aagcccgggg cgcacttccc tgtgcgcccg
     6301 ttc
//
LOCUS       DBNGZE010000276         2127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_455362, whole genome shotgun sequence.
ACCESSION   DBNGZE010000276 DBNGZE010000000
VERSION     DBNGZE010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_455362"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1222
                     /gene="ychF"
                     /locus_tag="AC16FV_07130"
     CDS             131..1222
                     /gene="ychF"
                     /locus_tag="AC16FV_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR2849865.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIDPNIGIV
                     EVPDPRLQQLAGIAKPEKILPTTIEFVDIAGLVAGASKGEGLGNKFLAHIREVDAIAH
                     VVRCFEDENIVHVSGGINPLADIETINTELALADLEAADKGVQRADKNAKSGNKDDIK
                     LRELLVRVHKHLDGGKPVRSMGLSEEEHRLLKPLCLLTDKPLMYIANVNEKGFSNNPW
                     LDQVDKLARSENSVVVPICARIEAELADMDAEEKKVFLADMGLEEPGLNRVIRAGYQL
                     LGLQTFFTVGPKEVRAWTVRKGAHAPQAAGVIHTDFERGFIRAETVSYEDFVRCKGEH
                     GAREAGKLRLEGKDYVVQDGDVMHFRFNV"
     gene            1305..1381
                     /locus_tag="AC16FV_07135"
     tRNA            1305..1381
                     /locus_tag="AC16FV_07135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Met,seq:cat)
     gene            1780..>2127
                     /locus_tag="AC16FV_07140"
     CDS             1780..>2127
                     /locus_tag="AC16FV_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR2849866.1"
                     /translation="MFQTSSGRSETKNTTCYMCACRCGIRVTLKDGEVRYIEGNPDHP
                     LNKGVICAKGASGIMKQHSPARLTRPLRRKPGAERGANEFEAISWDEAFAMLEERLAK
                     IRATDPKKFALFTG"
BASE COUNT          454 a          635 c          619 g          419 t
ORIGIN      
        1 gcggcggagc agacattaat tgacgcggct atcatgcatg gcaaggcgca cttgaccgag
       61 atcgtccacg gcgagttcca gaaggtcatg aacagcctgc acacgcacaa caagtaaacg
      121 agattccaag atgggtttca aatgcggtat tgtcggcctg ccgaacgtag gcaaatccac
      181 gctgttcaac gcgctcacca aggcgggcat cgccgcggag aattatccct tctgcaccat
      241 cgatccgaac atcggcatcg tcgaggtgcc ggacccgcgc ctgcaacaat tagctggcat
      301 cgccaagccg gaaaaaattc tccccaccac cattgaattc gtggacatcg cgggcctcgt
      361 agccggcgcc tccaagggcg aggggctggg caacaagttc ctcgcccaca tccgcgaggt
      421 ggacgccatc gcgcatgtcg tgcgctgttt cgaggacgag aacatcgtgc atgtttcggg
      481 cggcatcaat cctctggcgg acatcgaaac catcaacacc gaactcgcgc tcgccgatct
      541 cgaggcggcc gacaagggcg tacagcgcgc cgacaagaat gccaaaagcg gcaacaagga
      601 cgatatcaag ctgcgtgagc tgctggtccg cgtgcacaaa catctggatg gcggcaagcc
      661 ggtgcgcagc atgggcttga gtgaagagga acacaggctg ttgaagccac tgtgcctgct
      721 gaccgacaaa ccgctcatgt acatcgccaa cgtgaacgag aagggtttca gcaacaaccc
      781 ttggctcgat caggtggaca agctcgcgcg ctcggagaat tccgtcgtgg tgccgatctg
      841 cgcccgaatc gaggcggaac tggccgacat ggacgcggag gaaaagaagg tatttctcgc
      901 cgacatgggg ctggaggagc cgggcctgaa ccgcgtcatc cgcgccggtt accagttgct
      961 cggcttgcag acatttttta ccgtcggccc caaggaagtg cgcgcatgga ccgtgcgcaa
     1021 gggagcgcat gcgccgcaag ccgcgggcgt gatccacacc gatttcgagc gcggattcat
     1081 ccgcgccgaa accgtttcct acgaggattt cgtccggtgc aagggcgagc acggcgcgcg
     1141 cgaggccggc aagctgcgcc tggaaggcaa ggattacgtg gtgcaggacg gcgatgtcat
     1201 gcacttccgc tttaatgtgt agcgggatgg ttctatagtc aggccaccac ggcgtgattc
     1261 ccttgtcagc ctccgcggat atcggtatct tttgcggccc tttcggctag gtagctcaga
     1321 cggttagagc gcggcactca taatgctgag gtcggcggtt cgattccgcc cctagccacc
     1381 atcttctacc gacgaataac cagttttctt gtttttgttg ggaaccgccg acagctaatg
     1441 aatgctgagg tcgtcgcttg atgcgcgcca tccctggcgc gcaccctccg ggcggcctgc
     1501 ggccgtccaa ttttgttcct gacaaaattg tcgattccgc ccctagccac catcttcatt
     1561 tatctcgtgc ccactatttg tgtgagcctt ctttaagcag ttctcggcaa aaaatcgaaa
     1621 cacgagcttg tgtgcggtga tccattcgcc acatgagacc aggacatttc aaatcttatt
     1681 gccgcatcga atattgttaa ttgcgtgtaa catgcgggct tagtatcgta gctggcattc
     1741 gtatccttgt gcccgaccat ctggattggc ccagctccca tgttccaaac ctcttctggc
     1801 cgtagcgaga ccaagaacac gacctgctat atgtgcgctt gccgctgcgg tatccgcgtg
     1861 acgctcaagg acggcgaggt gcgctacatc gagggcaatc ccgatcaccc gttgaacaag
     1921 ggcgtcatct gcgccaaggg tgcctcgggc atcatgaagc agcattcgcc cgcgcgcctg
     1981 acccgaccgc tccgccgcaa gccgggcgcc gaacgtggcg ccaacgagtt cgaggcgatt
     2041 tcctgggacg aggcgttcgc gatgctggaa gaacgcctcg cgaaaatccg cgccaccgat
     2101 ccgaaaaagt tcgcgctgtt caccggg
//
LOCUS       DBNGZE010000277         7690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1567396, whole genome shotgun sequence.
ACCESSION   DBNGZE010000277 DBNGZE010000000
VERSION     DBNGZE010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1567396"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16FV_07145"
     CDS             complement(<1..296)
                     /locus_tag="AC16FV_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849867.1"
                     /translation="MNCRIIYLHRLEFTGSGQTIQVLRDYDSMLRHGHEVHLFYRAAR
                     EMSGTELGEALARHGLPPETGLQFHCITEGWNGKRRLYRDADALVGASDFPVMLV"
     gene            complement(318..1415)
                     /locus_tag="AC16FV_07150"
     CDS             complement(318..1415)
                     /locus_tag="AC16FV_07150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2849868.1"
                     /translation="MHIVHTEASRGWGGQEIRILTEAEGMIRRGHRVTLLCPPEARIL
                     SEAQQRKIPAVALPIGRKRLPGVLALRRWLKANPIDVINTHSSTDTWLVALASRLLER
                     PPPLVRTRHISAPVPKNRLTRWLYQKATTHIVTTGEKLRLQLIMENGYDPATITSVPT
                     GMDTGYFVPGVRAVRRAQLLLDGNALVIGIVATLRSWKGHEYLIEAFSRLSARSSQLV
                     ILVDGPQREVLHQRVQALGLHDRVFMPGNQRDVLPWLQAMDIFVLPSYANEGVPQAIM
                     QAMLCALPVVTTPIGSIGEIVTHDSTGLMVKPKDADELRQALERLAADASLRNRLGNQ
                     ARQFALAHFGLDPMLDKMEKIFAGAMAAPRR"
     gene            complement(1420..2382)
                     /locus_tag="AC16FV_07155"
     CDS             complement(1420..2382)
                     /locus_tag="AC16FV_07155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR2849869.1"
                     /translation="MKFIVNYRNFVPIREGLESCGYTVLENLWNPSDEQLRDCDGYLI
                     DMYDAMKRPWDTLRLKSRLHRRQIPLIGWNRDAPWNKGAKRRRLWWFRTLRVLDIYMT
                     HSLQGAATFSSEVHYLPNAARVLRYNLAGRTLEELRDSQGYRYDVSFVGTLDAQKYPE
                     FTERAKFFAELERRLTGFGVSTWFRHGKDMPLKDQIDLIQRSRINLTFGAACDDGPEK
                     SWGLPERCYGVPACGGFLLSDARVHAKDDFDIGKEWVDFSSMEDCVGKVRHYLANFPK
                     ARDIAGRAYQRVCREHTYEHRARTLVAAAREWKARAESRAAKTG"
     gene            complement(2379..3503)
                     /locus_tag="AC16FV_07160"
     CDS             complement(2379..3503)
                     /locus_tag="AC16FV_07160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2849870.1"
                     /translation="MLNIALIRQRYAQDGGAERFVARALDSLKTQNVRLTLVTREWRG
                     GNGFEVLVCRPFHIGRLWRDWAFARCACRTLAAGKFDLVQSHERIACCDIYRAGDGVH
                     REWLRQRSRVLGWTGRLGLALNPYHIYVKAAERALFTSSRLKAVICNSRMVRDEIRKY
                     FRLPDDKLPVIYSGVDTSVYHPDLKKHRAEIRARHAIPESATLFLFVGSGFERKGVPA
                     LLQAMTRLPENAFLLVVGRDKHADRFQRRAHALGLSGRVFFAGAQGDVKPYYGAADAL
                     TLPTLYDPFPNVALEAMASGLSVVTSLKCGAAELIENGKNGFACDALDIEGQAGFMRT
                     LLDAETRQRMGQAARRTVEPLTLAAMSDRLVQLYRGLVPA"
     gene            complement(3543..3956)
                     /locus_tag="AC16FV_07165"
     CDS             complement(3543..3956)
                     /locus_tag="AC16FV_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR2849871.1"
                     /translation="MEGNKNHDKEPTEAIDALVRKTLAAAVEVHRHLGPGYLESVYEE
                     AFATELSMQTIPFRRQIEVPVRYKNHLVGQGRLDFLVNGELVVELKAVDALLPIHKAQ
                     ILSYLKATQHRLGLLINFNVPVLMRGVQRIIYSNE"
     gene            complement(4007..5080)
                     /gene="rfaQ"
                     /locus_tag="AC16FV_07170"
     CDS             complement(4007..5080)
                     /gene="rfaQ"
                     /locus_tag="AC16FV_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HIR2849872.1"
                     /translation="MLKDAVDLSTLHRVLVIKLRHLGDVLLTSPVFTVLKNHAPQLEI
                     DALVYRDTEPMLSGHPAIANVFTIDRGWKQEEWTSRMANEWRLFRSLRARHYDLIIHL
                     TENSRGAVLSLLLKPHYSVARDYPAKRGRLWRNSFTHLYQSPDKRHTVEMHLDALRRL
                     GVYPRADERRLVLTTGVEAERSVRDLLARHGLTPRGFIHLHPTSRWMFKCWDADKNAA
                     LITALQEAGEPVVLTAAPSEPELQFAARITARLKKPVVDLSGKLNLKQLAALTAQAKC
                     FVGVDSVPMHIAAAVQTPCVAMFGPSNELIWGPWQVRHRVLTLPYSCRPCGIDGCGGG
                     KVSECLTELPVNSVLNAINEICD"
     gene            complement(5111..6124)
                     /gene="waaF"
                     /locus_tag="AC16FV_07175"
     CDS             complement(5111..6124)
                     /gene="waaF"
                     /locus_tag="AC16FV_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR2849873.1"
                     /translation="MANITQRLLIIGPAWVGDMVLAQSLFRVLRQRHPDARLEVLAPT
                     WTHPLLARMPEVDEAIAAPFVHGRLDLGARFRLGRELRARHYDRAIVLPNSLKSAIVP
                     FIARARVRTGFTGELRYGLLNDARKLDKQKLPRTVDRFVALALNSNEALPEIPLPRLR
                     ADTDKARAALSRLDRGLPAVPVLGLCPGAEYGPAKRWPAEYFAEVAKAGLAKGWEVWL
                     FGSSRDKPETGAVQSLTQGRCLDLAGKTTLEEAIDLLSLTTAVVTNDSGLMHVAAALD
                     RPVVALYGSSNPKHTPPMSAKATVLYLGLSCSPCFERECPLGHLNCLRQLGPERVLAA
                     LKG"
     gene            6465..7229
                     /locus_tag="AC16FV_07180"
     CDS             6465..7229
                     /locus_tag="AC16FV_07180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2849874.1"
                     /translation="MNDRYATKEIVREQAFRPIWSNELNEVFSDVARYYDRANVFATL
                     GLLNRLRNRFISTIDIQPNHKVLDVCAGTNVIGISLLKRQPDLDVYAVDRSVAMQEVG
                     RDRARRHGFRIKPFICDVHQLPFPDNYFDVVTLQWATRHLRVIEVFSEINRVLKPGGH
                     FHHCDMLRPANRLVEEIYYLYLKAVMGIIPRVFRSGPASMKCRNYFIEAIRLFYSTEE
                     LSTMLSELGFSHITGHSVLGGTVGIHKARKPQLDAA"
     gene            complement(7453..7656)
                     /locus_tag="AC16FV_07185"
     CDS             complement(7453..7656)
                     /locus_tag="AC16FV_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIR2849875.1"
                     /translation="MAANPKEQKLIEPNAQNRYEVTRADLPLSCPMPGMTLWNSHPRV
                     YLPIEESGEERCPYCGAVYVLKD"
BASE COUNT         1531 a         2406 c         2316 g         1437 t
ORIGIN      
        1 accagcatga cgggaaaatc gctcgcgccg accagcgcat cggcgtcgcg gtacaggcga
       61 cgctttccgt tccatccttc ggtgatgcaa tgaaactgca aaccggtttc cggcggcaat
      121 ccatgtcgtg ccagcgcctc tcccagctcg gtgccggaca tctcgcgcgc ggcccgataa
      181 aacaaatgca cctcgtggcc atggcgcagc atggaatcgt aatcccgcag cacctggatg
      241 gtctggcccg agcccgtgaa ctccaggcgg tgcaggtaaa tgatccggca gttcatgcgg
      301 aatgtgcgcg tgagcttcta gcggcgcggc gcggccatgg cgccggcgaa aatcttttcc
      361 attttgtcga gcatcgggtc cagcccgaaa tgcgccagcg caaactgccg tgcctgattt
      421 cccagccggt tgcgcaggct ggcgtccgcg gcgagtcttt ccagcgcctg ccgcagctcg
      481 tcggcgtcct tcggcttcac catcaggccg gtggaatcgt gcgtgacgat ctcgccgatg
      541 ctgccgatcg gggtcgtcac caccggcagc gcgcacaaca tcgcctgcat gatggcctgc
      601 ggcacgccct cgttggcgta ggacggcagc acgaaaatat ccatggcctg caaccacggc
      661 aggacgtcgc gctgattgcc cggcatgaac acgcggtcgt gcagacccag ggcctgcacg
      721 cgctggtgca acacctcgcg ttgcggcccg tcgaccagta tcaccagctg cgacgaccgc
      781 gcggacaggc ggctgaatgc ctcgatcagg tattcatggc ccttccagct gcgcagggtc
      841 gcgacaatcc cgatgaccaa cgcgttaccg tcgagcagca actgcgcgcg ccgcacggcc
      901 cggacgccgg gaacgaaata tccggtgtcc attcccgtcg gcaccgaagt gatcgtggcc
      961 gggtcatagc cgttctccat aatgagctgc aggcgcagct tttcgccagt ggtgacgata
     1021 tgcgtggtcg ccttctggta cagccagcgc gtcagccggt ttttcggcac cggcgccgag
     1081 atgtggcgcg tgcgcaccag cggcggcgga cgctcgagca ggcgtgaagc cagcgccacc
     1141 agccaggtgt cagtcgagct gtgggtgttg atcacatcga tgggattggc cttgagccag
     1201 cggcgcaggg ccagaacgcc cggcaagcgc ttgcgcccga tcggcagcgc cacggcggga
     1261 atcttgcgct gctgcgcctc ggaaagaatg cgcgcctcgg gcgggcacaa cagcgtcacc
     1321 cggtgcccgc ggcggatcat gccttccgcc tcggtaagga tgcgtatttc ctgtccgccc
     1381 cagccgcgcg aggcctcggt gtgaacgata tgcatggcgt cagcccgtct tcgccgcgcg
     1441 gctttccgcc cgcgccttcc attccctggc ggcggccacg agcgtgcggg cgcggtgctc
     1501 ataagtatgc tcccggcaca cgcgctggta cgcgcgcccc gcgatatcgc gcgcctttgg
     1561 aaagttcgcc aaataatgcc tgaccttgcc gacgcaatct tccatgcttg aaaaatccac
     1621 ccattccttg ccgatgtcga aatcatcctt cgcgtgcacc cgcgcatcgc tcagcaggaa
     1681 tccgccgcac gcgggcacgc cgtaacagcg ctcgggcagg ccccagctct tctcgggacc
     1741 gtcgtcgcag gccgcgccga aagtcagatt gatgcggctg cgctggatca agtcgatctg
     1801 atccttgagc ggcatgtcct tgccgtggcg gaaccacgtc gagacaccga aaccggtcag
     1861 acgccgttcc agctcggcaa aaaatttcgc gcgttcggtg aactcgggat atttctgcgc
     1921 atccagggta ccgacaaacg aaacatcata acggtagccc tgcgaatcac gcagctcctc
     1981 cagcgtgcgt ccggcgaggt tgtagcgcaa gacccgcgcg gcattgggca ggtagtggac
     2041 ctcggacgaa aacgtcgcgg ccccctgaag actgtgcgtc atatagatat ccagaacacg
     2101 cagtgtgcga aaccaccaca gccggcggcg tttggcaccc ttgttccacg gcgcgtcgcg
     2161 attccatccg atgagcggga tctggcgccg gtgcaggcgc gacttgagcc gcagcgtgtc
     2221 ccacgggcgt ttcatggcgt catacatgtc gatcagatag ccgtcgcagt cccgtagctg
     2281 ctcgtccgac ggattccaca gattttcaag aactgtatag ccacaacttt ccaggccttc
     2341 gcgtatcgga acgaagttgc ggtaattgac gatgaacttc atgccgggac cagtccccgg
     2401 tagagctgca ccagcctgtc gctcatggcc gcgagcgtca acggctctac tgtccgtcgc
     2461 gcggcctgtc ccatgcgttg ccgggtttcc gcatcgagca gggtacgcat gaagcccgcc
     2521 tgtccctcga tatccagggc atcgcaggca aaaccgtttt tcccgttctc gatcaactcg
     2581 gccgcgccgc acttgagact ggtgacgacg gacaggccgc tggccatcgc ctccagcgcc
     2641 acgttgggaa atggatcgta gagcgtgggc agggtcagcg catcggccgc accataataa
     2701 ggtttgacgt caccctgggc cccggcaaag aacacgcgtc cggacaggcc gagcgcatga
     2761 gcgcggcgtt ggaaccggtc cgcatgcttg tcccgcccca ccaccagcaa aaaggcgttc
     2821 tccggaagcc tggtcatggc ctgcaacaac gccggcactc ccttgcgttc aaaaccggaa
     2881 ccgacgaaca aaaacaatgt tgcgctctcc ggtatggcat ggcgcgcgcg aatttccgcg
     2941 cggtgctttt tcagatcggg atggtagacc gaagtatcga ccccgctgta tatcacgggc
     3001 aatttgtcgt ccggaaggcg gaaatatttc ctgatctcgt cgcgcaccat gcgtgaattg
     3061 cagatcaccg ccttgagccg cgagctggtg aacaaggccc gttccgccgc cttcacataa
     3121 atatgatagg gattgagcgc gagacccagc cggccggtcc agccgaggac gcgggatcgt
     3181 tgcctcaacc attcacgatg cacgccgtcg ccggcgcggt agatgtcaca acaggcgatg
     3241 cgttcatgcg actgaaccag atcaaatttt cccgcggcca gcgtccggca ggcgcaacgc
     3301 gcaaacgccc agtcgcgcca cagcctgccg atatgaaacg ggcggcacac caacacctcg
     3361 aagccgttgc cgccacgcca ttcgcgcgtc accagggtca ggcggacgtt ctgtgtcttg
     3421 agcgagtcga gtgcgcgagc gacgaaacgc tccgcgccgc cgtcctgcgc atagcgctgt
     3481 cgtatgagag cgatattcag catatgaatt tgaaccgcca ggacgccagg agcgccagga
     3541 tattattcat tcgaatagat gatgcgttga acaccgcgca tcaatacggg gacattgaag
     3601 ttaataagca acccgagtcg atgttgggtc gccttcaaat aagaaagtat ttgggcttta
     3661 tgtatgggca agagtgcgtc aaccgcttta agttccacaa ccagctcgcc attaaccaaa
     3721 aaatccagac gaccctgtcc cacgagatga ttcttatatc gaactggaac ctcgatctgg
     3781 cggcggaacg gaattgtttg catggataac tcagtggcaa aagcttcttc atagactgat
     3841 tcgagataac ctgggcccaa atgcctatgc acttcgacag cggctgctaa tgtcttgcgg
     3901 accaaagcat caattgcttc ggttggttcc ttatcgtgat ttttatttcc ctccatattt
     3961 tcctggcgct cctggcgccc tggcggttca tttatcaatt atatcgttaa tcgcatatct
     4021 cgttaatcgc attcaagacg ctattcacgg gcagttcggt aaggcattcg ctcaccttgc
     4081 cgccgccaca accgtcaatg ccgcagggcc ggcaggaata cggcagggtc agaacgcgat
     4141 ggcgcacctg ccacgggccc cagatcagct cgttgctcgg tccgaacatg gcgacacagg
     4201 gcgtctgcac cgccgccgcg atgtgcatgg gcacggaatc cacaccgacg aaacatttgg
     4261 cctgtgccgt cagggccgcg agctgcttga gattgagctt gccggacaga tccaccaccg
     4321 gcttcttcag gcgcgcggtg atgcgcgcgg cgaactgcag ctcgggctcg ctgggcgcgg
     4381 cggttagcac caccggctcg cccgcctctt gcaacgcggt gataagcgcg gcgttcttgt
     4441 ccgcgtccca acatttgaac atccaacgcg acgtgggatg gagatgaata aaacccctgg
     4501 gcgtcaggcc gtgacgcgcc agcaggtcgc gtaccgagcg ctcggcctcc acgccggttg
     4561 tcaataccag gcgacgttca tccgcgcgcg ggtaaacacc gagccggcgc agggcatcca
     4621 gatgcatttc cacggtgtgc cgcttgtccg gagactggta cagatgggtg aaactgttgc
     4681 gccacaggcg tccgcgcttg gccggataat cgcgcgccac gctgtaatgc ggtttcagca
     4741 gcagactgag caccgcgcca cgggaatttt ccgtgaggtg gatgatgagg tcgtagtgcc
     4801 gggcgcgcag ggaacggaac aggcgccact cattcgccat gcgcgaggtc cattcctcct
     4861 gcttccagcc gcggtcaatc gtgaaaacgt tcgcgatcgc gggatgaccc gacagcatcg
     4921 gctcggtgtc gcgatacacc agcgcgtcga tctcgagctg cggggcgtga ttcttgagga
     4981 ccgtgaaaac cggggaggtc aggagcacgt cgccgaggtg gcggagcttg atcaccagca
     5041 cgcggtgcag ggtggagagg tcgacggcgt ccttgagcat cgcgccaacg atagcagatt
     5101 tttttccggc ttaccccttg agcgccgcga gcacgcgctc gggcccgagc tgacgcaggc
     5161 agttgagatg ccccaacgga cactcgcgtt cgaagcacgg gctgcacgaa agaccgagat
     5221 aaagcaccgt ggccttggcg ctcatgggcg gcgtgtgttt cgggttcgac gaaccataga
     5281 gggccacgac cgggcggtcg agcgccgcgg ccacgtgcat gaggcccgaa tcgttggtga
     5341 ccacggcggt ggtcagcgac aacaaatcaa tcgcctcctc cagcgtggtc ttgccggcga
     5401 gatcgagaca acgaccctgc gtcagggatt gaacggcgcc ggtttcgggt ttgtccctgc
     5461 tcgaaccaaa cagccacacc tcccagccct tggcgagtcc cgccttggcc acctcggcaa
     5521 aatattccgc cggccagcgc ttggccgggc cgtattcggc gccgggacac agacccagca
     5581 ccggtaccgc aggcagaccg cgatccaacc gcgacaacgc ggcgcgcgcc ttgtccgtgt
     5641 ccgcccgcaa ccgtggcaag ggaatttccg gcagcgcctc attcgaattc agcgcgagcg
     5701 cgacaaagcg gtccacggtg cgcggaagtt tctgtttgtc gagcttgcgt gcatcgttga
     5761 gcagtccgta acgcagctcg cccgtgaagc cggtgcgaac acgggcacgt gcgataaaag
     5821 gaacgatcgc ggacttgagc gagttgggca acacgatagc gcggtcgtaa tgacgtgcgc
     5881 gcaattcacg accgagacgg aaccgcgcgc cgagatcaag acggccgtgg acaaatggcg
     5941 cggcgatcgc ctcgtccacc tccggcatgc gcgccagcaa tggatgggtc caggtgggcg
     6001 ccagcacctc gagacgcgca tccggatgcc gctgcctgag caccctgaac aggctttgcg
     6061 cgagcaccat gtcgccgacc caggccgggc cgataatcag taagcgttgc gtaatgtttg
     6121 ccaagcgctg ttacggcttg ccgcgctgag ggacaaaagg cgtcgaacga tgattcttcg
     6181 tggctatcgc gcgcatccgg cagattctag gcaggaccaa aatcgggttc aaccaaaaca
     6241 gatcgaccga ttaaaatgca cgcattgtgc tgggaaccgc ggccaaactc gctatcgcct
     6301 ttgccatgct gggaatattc gccattgtcc gttttaccgg gggtaactag cgcgccgtca
     6361 gggaaattga aataaatcaa atcatcgggc gaccggattc gcctattatt cgggtggcct
     6421 cccgcgcatg gaccgggacg accggcccga aacagaggaa cgacatgaac gaccgttatg
     6481 ccacaaagga aattgtccgc gaacaggcgt ttcggcccat ctggtcgaat gagctgaacg
     6541 aagttttttc cgacgtcgcc cgctattacg accgcgccaa tgtcttcgcc acgcttggcc
     6601 tgttgaaccg gctgcggaat cgtttcattt ccaccatcga cattcagccc aaccacaagg
     6661 tgctggacgt gtgcgccggc accaacgtga tcggcatcag ccttttaaaa cgccagccgg
     6721 atctcgatgt ctatgcggtg gaccggagcg tggcaatgca ggaagtcggt cgagaccgcg
     6781 cccggcgcca tggtttccgg ataaagccct tcatctgcga cgtgcaccag ctgcctttcc
     6841 ccgataatta ttttgacgtg gtgaccttgc aatgggcgac gcggcatttg cgcgtcatcg
     6901 aggtgttctc cgagataaac cgcgtgctca agcccggcgg gcatttccac cactgcgaca
     6961 tgctgcggcc ggccaacagg ctggtggagg agatctacta cctgtacctg aaagctgtca
     7021 tgggcattat tccacgcgtc ttccgcagcg ggccggcatc catgaagtgc cgcaactact
     7081 ttatcgaggc catccgcctg ttctactcga cggaagagct ttccaccatg ctctcggaac
     7141 tgggcttctc ccacatcacg ggtcattcag ttctgggtgg aacggtgggg attcacaagg
     7201 cgcgcaagcc acaacttgat gccgcctgaa cagccacggg attgccccgc ggagaaatta
     7261 gttgaagcta tctcaaaaac gtcgcgagcg gccgtcagcc gggtgagggt gaggcggaga
     7321 tttcacgaac gcccggtgga cgttcgtgcc accgaacggg ccgccgccag ggatggcggc
     7381 cctggacttt cccgcggagc cggatggcac agcggaaagg agcgcagcgc gcagcaggtt
     7441 ttgagatagc ttctagtcct tgaggacgta taccgcgcca cagtagggac agcgctcctc
     7501 cccgctttcc tcgattggca ggtatacccg cgggtgtgaa ttccacagtg tcatgcccgg
     7561 catcgggcag gacagcggca gatcggcgcg cgtgacctca tagcggttct gcgcgttggg
     7621 ctcgatcagc ttctgctctt tcgggttcgc ggccatagca tccatcctca cgccaccggc
     7681 gtcagccatt
//
LOCUS       DBNGZE010000278         7388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1428707, whole genome shotgun sequence.
ACCESSION   DBNGZE010000278 DBNGZE010000000
VERSION     DBNGZE010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1428707"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16FV_07190"
     CDS             <1..212
                     /locus_tag="AC16FV_07190"
                     /inference="COORDINATES: protein motif:HMM:NF044651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849876.1"
                     /translation="QAGNGFVEELKEILSPAAQGKCPVYLHYQGRQAEAEIVLGDEWR
                     ISPTNTLLERLSRLAGESNVHLIYR"
     gene            218..1177
                     /locus_tag="AC16FV_07195"
     CDS             218..1177
                     /locus_tag="AC16FV_07195"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR2849877.1"
                     /translation="MNQNYLEFEQSIADLEAKIEALRYADKGGAININEEINKLSAKS
                     RKLTESIFHALTPWQISQLARHPLRPYTLDYLARAFTDFEELHGDRAFSDDPAIVGGL
                     ARLDGRPVVVIGHQKGRDTREKVRRNFGMPRPEGYRKALRLMRIAERFHLPLITLIDT
                     PGAYPGVGAEERGQSEAIARNLYEMARLHVPIVSVVIGEGGSGGALAIGVCDRLMMLQ
                     YSTYSVISPEGCASILWRSADKAAEAAQAMGITAESLSRQGLVDEIVPEPLGGAHRDM
                     DEVAKILKSSLIAALDNLNSVPLERLLERRYDRLMHYGEFEEH"
     gene            1158..2570
                     /gene="tilS"
                     /locus_tag="AC16FV_07200"
     CDS             1158..2570
                     /gene="tilS"
                     /locus_tag="AC16FV_07200"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR2849878.1"
                     /translation="MNLKNIDDVQGRTNVAGDRMSGATPAFSPSALADVLHRLGLSSA
                     TPLKVAFSGGLDSCVLLHALSRLREALDLQLHAIHVDHGLHPDSSDWARRVQQTCERL
                     NISCTVERVQVTRIREHGLEDAARRVRYACLARHVGPDEVLLTAHQADDQAETVMLQL
                     LRGTGAHGLAAMPAMAAFYRGRLARPLLDFTRKQLESYARQENLQWIDDSSNKDPRFS
                     RNYLRHQIFPLLEAHWPEASRRIARSASMVAEAAELLDELAKSDLNGCCGADEAKLSV
                     NALRGLTPPRRRNLIRYWLRRQGFQMPTAMHLDQVLAQVMRDPHSNQAVIRWSGTEVC
                     RYRDELMAHKPRVEPDSGWRIGWNLPDALEIPGVGLLRVELSHGDGLSQERIARAPLS
                     VGLRQGGETCRLPGRAHHHKLKKLLQEAGIPPWERKRLPLVYVNGELAAIGDRWVCEP
                     YAARANEPGWKLRLDPALSE"
     gene            2783..4438
                     /locus_tag="AC16FV_07205"
     CDS             2783..4438
                     /locus_tag="AC16FV_07205"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR2849879.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLGTILEARGLKVTLLKLDPYI
                     NVDPGTMSPYQHGEVFVTQDGAETDLDLGHYERFVRTTMTKRNNFTTGKIYENVIRKE
                     RRGDYLGATVQVIPHITDEIKRCIQQGASEADVALVEIGGTVGDIESQPFVEAIRQMG
                     VEHGPSNVVYLHLTLVPYIAAAGELKTKPTQHSVKELLSLGIQPDILLCRADRPLPED
                     ERRKIALFTNVPTRAVITAMDVDNIYKIPRLLHEQGLDEIVVEKLHLNARRANLAEWD
                     KVVHAMEHPTGEITVAMVGKYMHLTESYKSLSEALKHAGLHTLTRVNIRYVDSEQIDA
                     QGTGQLEDVDAILVPGGFGERGIEGKIKAVQYARTKGIPYLGICLGMQVAVIEYARNV
                     AGLKGAHSTEFNPKTPYPTIAMITEWTTAEGAKEKRREGDDLGGTMRLGGQECKLMPG
                     THVRGLYGKDTIVERHRHRYEFNNHYREILQEKGLVIAGTSMDNMLVEIIELKDHPWF
                     VGVQFHPEFTSTPRDGHPLFTGYVQAARARRSQDVPRTQVAAT"
     gene            4435..5280
                     /gene="kdsA"
                     /locus_tag="AC16FV_07210"
     CDS             4435..5280
                     /gene="kdsA"
                     /locus_tag="AC16FV_07210"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR2849880.1"
                     /translation="MKLCGFEAGLNRPFFLIAGPCVIESEALALDTAGRLKEITQSLG
                     IPFIYKSSYDKANRSSSKSYRGLGMEQGLAILANVRKQIGVPVLTDVHDAEDVAAVAA
                     AVDVLQTPAFLCRQTDFIQAVVAAGKPVNIKKGQFLAPHDMKNVVDKAVEAGGRERIL
                     VCERGVSFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTKSGGQREFVPVL
                     ARAAVATGVAGLFMETHPDPDKALSDGPNAWPLGRMKDLLETLKELDAAVKRRGFAET
                     TLMPN"
     gene            5482..6777
                     /gene="eno"
                     /locus_tag="AC16FV_07215"
     CDS             5482..6777
                     /gene="eno"
                     /locus_tag="AC16FV_07215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR2849881.1"
                     /translation="MSVINDIRAREILDSRGSPTVEADVILKSGAVGRAAVPSGASTG
                     TREAIELRDGDARRYGGKGVKNACAHVNGEIRKALLGKDAMDQAGVDRVMVELDGTDN
                     KSRLGANALLAVSLACARAAASDAKQPLYRYLNGLAGNVPMQMPVPMMNIINGGVHAD
                     NNIDFQEFMILPVGAGSFAEALRCGAEVFHSLKKVLQGMKLNTAVGDEGGFAPDLKSN
                     EEALQVILQGIEKTGYKPGTDVCLGIDVASSEFNKDGKYELESEHASLTAEQFIDKLA
                     GWVGKYPIITIEDACGEADWAGWELLTKKLGRKIQLVGDDLFVTNTSILERGIKQGIA
                     NSILIKVNQIGTLSETLAAIAMARKAGYTSVISHRSGETEDSFIADLAVATGTGQIKT
                     GSLSRSDRVAKYNQLLRIEEEMGSKAGYPGRRACPMLAG"
     gene            6824..7138
                     /gene="ftsB"
                     /locus_tag="AC16FV_07220"
     CDS             6824..7138
                     /gene="ftsB"
                     /locus_tag="AC16FV_07220"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HIR2849882.1"
                     /translation="MKIATYVLLGILVLLQYPLWFGDGGVIAVWRLNREIAAQQKENA
                     QLKDRNQTLEAQVNDLKQGLEVIEGRARAELGMVKKGETFYQVIEQPRDKPPPADKKT
                     TP"
     gene            complement(7140..>7388)
                     /locus_tag="AC16FV_07225"
     CDS             complement(7140..>7388)
                     /locus_tag="AC16FV_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2849883.1"
                     /translation="EQEQQAEAARKQFETTVNGMPAATFSVEAGDPAEVVLQLANKPS
                     TDLLVLSRRVFQVSGRPSLKTLGEKLARKATRPFLLYP"
BASE COUNT         1520 a         2085 c         2297 g         1486 t
ORIGIN      
        1 accaggcggg caacggcttc gtcgaggagt tgaaggaaat tctcagcccc gcggcgcagg
       61 gcaagtgccc ggtgtatctg cattaccagg gccggcaggc ggaagccgag atcgtgctgg
      121 gcgatgagtg gagaatcagc ccgacgaata cgctgctgga aaggttgtcg cgccttgccg
      181 gcgagagcaa cgtacatttg atatatcgct gataaacatg aatcagaatt atctcgaatt
      241 cgagcagtcc atcgccgatc tggaagccaa gatcgaagcc ttgcgctatg ccgacaaggg
      301 cggggcgatc aatatcaacg aagagatcaa caagctcagc gccaaaagcc gcaagctgac
      361 cgaatccatt tttcatgcgc tgacgccatg gcagatttca cagctggcgc gccatccact
      421 gcgcccctat acgctggatt atctggcgcg cgcctttacc gatttcgagg agctgcacgg
      481 cgatcgcgcc ttctccgacg atccggcgat cgtcggcggg ctggcgcggc ttgacgggcg
      541 cccggtggtg gtcatcggac atcagaaggg gcgcgacacg cgcgagaagg tgcggcgtaa
      601 tttcggcatg ccgcgtccgg aaggttaccg caaggcactg cgtttgatgc gcatcgctga
      661 gcgcttccat ttgcctctaa tcacgctgat cgataccccc ggcgcttatc ctggcgtcgg
      721 cgcggaagag cgtggccaga gcgaggccat cgctcgcaat ctctatgaaa tggcgcggct
      781 gcatgttccc atcgtgagcg ttgtcattgg cgagggcggt tccggcggtg cgttggccat
      841 cggcgtgtgc gaccgattga tgatgttgca gtattcgact tattccgtca tctcgcccga
      901 aggttgtgcc tcgattctgt ggcgctccgc ggacaaggcc gcggaggcgg cgcaggccat
      961 gggcatcacc gccgaaagcc tgagccgcca gggcttggtg gatgagatcg tgccggaacc
     1021 gctgggcggc gcgcatcgcg acatggacga ggtggccaag atcctcaagt catccctgat
     1081 cgcggcgctc gacaacctga attcggtgcc gctcgagcgt ctgctggaaa ggcgctacga
     1141 tcgcttgatg cattacggtg aatttgaaga acattgatga cgtgcaggga cgcactaatg
     1201 tcgcgggaga caggatgtcg ggagcgacgc cggctttttc tccctcggca ctggcggacg
     1261 tcctgcaccg cctgggtttg tcctccgcga caccactgaa agtcgccttc agcggcggcc
     1321 tcgattcctg cgttcttttg catgcgctca gtcggttgcg cgaagcgttg gatttgcaac
     1381 tacacgcgat acatgtggat catgggctgc accccgattc ctccgattgg gcacgacgag
     1441 tccaacaaac ctgtgagcgt ctgaatattt cctgcaccgt ggaacgcgtg caggttacgc
     1501 ggatccgcga gcatggactg gaagacgccg cgcggcgtgt gcgctacgcc tgcttggcgc
     1561 ggcatgtcgg gccggacgag gtattgctca cggcgcatca ggcggacgat caggccgaga
     1621 ccgtgatgct tcagctgttg cgcgggacgg gtgcgcacgg tctggcggct atgccggcca
     1681 tggcggcgtt ttaccggggc cggctggcgc ggcccttgct ggattttacc cgcaagcaac
     1741 tggaaagtta cgccagacag gaaaatctgc aatggattga tgacagcagc aacaaggacc
     1801 cgcggttctc gcggaattac ctgcgtcatc agatttttcc attgctcgaa gcgcattggc
     1861 ctgaggcgtc acgccgaatc gcgcgctcgg cgagcatggt agcggaagcc gcggagctgc
     1921 tggacgagct ggcgaaatcc gacttgaatg gttgttgtgg cgctgatgaa gcaaaactat
     1981 cggtcaatgc cctgcgcgga ttgactccgc cgcgacggcg caatctcatc cgctattggt
     2041 tgcgtcgcca ggggtttcag atgccaacgg ccatgcatct cgatcaggtg ctggcgcaag
     2101 tcatgcgcga tccacactcg aaccaggccg tgattcgctg gtctgggacc gaagtgtgcc
     2161 gctatcgcga cgaactcatg gcgcacaaac cacgcgtcga accggattcc ggatggagga
     2221 ttggctggaa tttgccggat gcactggaaa ttcccggagt tggattgctg cgtgtggagt
     2281 tgtcgcatgg tgatggtttg tcacaagagc gtatcgcccg ggcaccgctc agcgtcgggt
     2341 tgcgccaggg tggggaaacc tgccgtttgc ccggccgtgc gcatcatcac aagctgaaga
     2401 aactgctgca ggaggccggc atcccgccgt gggagcgcaa gcgtttgccg ttggtgtacg
     2461 ttaacggcga acttgccgct atcggcgatc gctgggtttg tgaaccgtac gcggcgcgag
     2521 cgaacgagcc gggatggaaa ctgcggctcg atcctgcgtt atcggaatga atcaataaat
     2581 ataattttga accgccaggg cgccagggtc gccaggaaaa gcaattcttg gaaatttaca
     2641 tccctggcgt tcctggcgtc ctggcggttg attttgttct gttggggttt attggaataa
     2701 aaacggttgt gaaaacccct gccatctggt aacttgtgtt tccgttctgc ggcgttgttc
     2761 aactcctgct aaacgggccg aaatgaccaa atttgtcttt gtgaccggcg gcgtggtgtc
     2821 ctctctgggc aaaggcattg cctccgcatc gttgggcacc atcctcgaag cccgcggcct
     2881 caaggttacc cttctcaagc ttgatcccta cattaatgtc gatccgggca ccatgagccc
     2941 gtatcagcac ggcgaggtat tcgtcaccca ggacggcgct gaaaccgacc tggatcttgg
     3001 gcattacgag cgcttcgtgc gcactaccat gaccaagcgc aacaatttca ccaccggtaa
     3061 aatctacgaa aacgtcatcc gcaaggaacg tcggggcgat tatctgggcg ccacggttca
     3121 ggtcatcccg cacatcacgg acgagatcaa gcgctgcatc cagcagggcg cgagcgaggc
     3181 cgacgtggcg ctggttgaga tcggcggcac ggtgggtgac atcgaatcgc agccgttcgt
     3241 cgaggccatc cgccagatgg gtgttgagca cggtcccagc aatgtcgtgt atctgcacct
     3301 gacgctcgtg ccatacattg cagcagcggg agaactcaag accaagccga ctcagcactc
     3361 tgtcaaggag ttgctctcgc tcggcattca gccggacatt ctgctttgcc gcgcggatcg
     3421 cccgttgccg gaggacgagc gccgcaagat cgcgcttttc accaacgtgc ccacgcgcgc
     3481 ggtcatcacc gccatggacg tggataacat ctacaagatt ccgcggctgt tgcatgagca
     3541 ggggctggat gaaatcgtgg tggaaaaact gcatctgaac gcgcgccggg ccaacctggc
     3601 cgagtgggac aaggtggtgc acgccatgga gcatccgacc ggcgagatca ccgtggccat
     3661 ggttggcaaa tacatgcacc tcacggaatc ctacaagtca ttgtcggagg cgttgaagca
     3721 cgccggtctg cacacgctga cgcgtgtcaa tatccgctac gtcgattctg aacagattga
     3781 tgcgcaggga acgggccagc tcgaagacgt tgacgcgatt ctggtgccgg gcggttttgg
     3841 cgagcgcggg atcgagggca agatcaaggc ggtgcaatac gcgcgcacca agggaattcc
     3901 ctatcttggc atctgccttg gcatgcaggt ggccgtgatc gaatacgcgc gtaacgtcgc
     3961 cgggctcaag ggcgcgcaca gcaccgaatt caaccccaag acgccgtatc ccacgatcgc
     4021 catgatcacc gaatggacca cggcggaggg cgcaaaagag aaacgtcgcg agggtgacga
     4081 tctgggtggt accatgcgtc tgggcggtca ggagtgcaag ctgatgccgg gcacgcatgt
     4141 gcgcggcctg tatggcaagg acacgattgt cgaacgacac cgtcatcgct atgaattcaa
     4201 caaccattac cgcgagatat tgcaggagaa aggactggtg atcgctggaa cctccatgga
     4261 caacatgctg gtggaaatca tcgagctcaa ggatcacccg tggttcgtgg gcgtgcagtt
     4321 ccatcccgaa ttcacctcga caccccgcga tggccatccg ttgttcaccg gctatgtgca
     4381 ggccgcgcgg gcgcgccgct cccaggatgt gccgcgcacg caggttgccg cgacatgaaa
     4441 ctttgtggtt tcgaggccgg gctgaaccgg ccgtttttcc tcattgccgg accttgtgtc
     4501 attgaatccg aggcgctcgc gctcgatacc gccggccgcc tcaaggaaat tacccaaagc
     4561 cttggtattc cgttcatcta caaatcttcc tacgacaagg ccaatcgcag ctcatccaaa
     4621 tcctatcgcg gtctgggtat ggagcagggg ttggcgattc tggcgaatgt gcggaaacag
     4681 atcggcgtgc cggtgctgac cgacgtgcac gacgctgaag atgttgccgc cgtggctgct
     4741 gcggtggacg tgttgcaaac cccggcgttt ctgtgccggc agaccgattt tatccaggcg
     4801 gtcgtggccg ccggcaagcc ggtcaatatc aagaagggcc agtttctcgc gccgcatgat
     4861 atgaagaacg tggtcgacaa ggccgtcgag gccggtggcc gggaacgcat tctcgtgtgc
     4921 gagcgcgggg tgtccttcgg atacaataat ctggtttcgg acatgcgttc actggcgatt
     4981 atgcgcgaaa ccggctgccc ggtggtcttc gacgccacgc attcggtgca gttgccgggc
     5041 gggcagggga cgaagagcgg cggccagcgc gaattcgtgc ccgtgctggc gcgcgcggcg
     5101 gtggccacgg gtgtcgcggg gttgttcatg gagacccatc ccgaccccga caaggcgctg
     5161 tccgacggcc ccaatgcctg gccgttggga cgcatgaagg atttgctcga gacgctgaaa
     5221 gaacttgatg ctgcggtcaa gcgccgcggc tttgccgaga cgacgttaat gccgaattag
     5281 ttattatttt taccgccagg acgccagggc cgccaggaaa aacgattaag ctgtctcaac
     5341 acatcttgct acgaatacga cggctgatca gagagttttt atgtcttgat ttattttcaa
     5401 aaaatcattg agatattttt ttgatttcct ggcgtcctgg cggttcaaga tcaataatca
     5461 caacctttcc atataaaaac catgtccgtt attaacgata tacgagcgcg cgagatactc
     5521 gactcgcgcg gaagtcccac tgtggaagcc gatgtcatcc tgaagtccgg cgccgttggc
     5581 cgcgccgctg tgccttccgg cgcttctacc ggcacgcgcg aggccattga attgcgcgac
     5641 ggcgatgccc gacgctatgg cggcaagggc gtgaaaaacg cctgcgctca tgtgaatggc
     5701 gagattcgca aggccttgct cggcaaggac gccatggatc aggccggtgt ggaccgagtc
     5761 atggtcgagc tcgatggtac ggacaacaaa tcgcgtctcg gcgccaatgc gttgctcgcc
     5821 gtttcactgg cctgcgcgcg cgccgccgcg tctgacgcca aacagccgct ttaccgttac
     5881 ctcaatggtt tggcaggcaa cgtgccgatg cagatgccgg tgccgatgat gaacatcatc
     5941 aacggtggcg tgcatgctga caacaatatc gatttccagg agttcatgat cctgccggtg
     6001 ggtgcgggca gcttcgccga ggctttgcgc tgtggcgcgg aagtgtttca ttccctgaaa
     6061 aaagtgctgc aaggcatgaa gctcaacact gcggtcggcg atgagggcgg tttcgctccc
     6121 gatcttaaat ccaacgagga ggcgcttcag gtcattcttc aaggcatcga gaagacaggc
     6181 tacaagccag gcacggatgt gtgtcttggc atcgatgtcg ccagttctga attcaacaaa
     6241 gacggcaaat acgagctgga atcagagcac gccagtctga ccgccgaaca gttcatcgac
     6301 aagctggccg gctgggtcgg caaatatccg attatcacca tcgaggacgc ctgcggcgaa
     6361 gcggattggg cggggtggga gctgctgacc aaaaaactcg gcagaaaaat ccagctcgtc
     6421 ggcgacgatc tgttcgtcac caatacctcc attctggagc gtggcatcaa gcagggaatc
     6481 gccaattcca tcctgatcaa ggtgaaccag atcggcacct tgagcgagac cctggcggcg
     6541 atcgcgatgg cgcgcaaagc gggatatacc tcggtaattt cgcaccgctc cggggaaacc
     6601 gaggacagtt tcatcgccga tctcgcggtg gcgaccggca ccggccagat caagaccggc
     6661 tcgctatcgc gttccgatcg cgtggccaag tacaatcagc ttttgcgcat cgaggaagaa
     6721 atgggctcga aggccggtta tcccggccgc cgggcgtgtc ccatgctggc aggctgagtc
     6781 gccgactcag gcggcaggtg ttgcgtaggc tagaatgaat tttgtgaaga tagccacata
     6841 tgtcttgctc gggattcttg tcctgctgca atatccgctg tggtttggcg atggcggggt
     6901 gatcgctgtg tggcgtctca atcgggaaat tgccgcgcag cagaaggaaa acgcccaact
     6961 gaaggaccgc aatcagacgc tcgaggcgca ggtcaacgac ctgaagcagg ggctcgaagt
     7021 aatcgaaggc cgcgcgcgtg ccgagcttgg catggtcaag aagggagaga ctttttatca
     7081 ggtgatcgag cagccgcgcg acaagccgcc gccggcggac aaaaaaacga caccttgaat
     7141 tacggataca gcagaaacgg ccgggtcgcc tttctggcca gtttttcacc cagggtcttg
     7201 aggctgggcc gcccgctgac ctgaaacacc cgccggctca acaccagcag atcagtcgaa
     7261 ggcttgttcg cgagttgcaa caccacctcc gccgggtcgc ccgcttccac ggaaaatgtc
     7321 gccgcgggca tgccgttgac cgtggtttca aactgtttgc gcgcggcctc ggcctgctgc
     7381 tcctgctc
//
LOCUS       DBNGZE010000279         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1076402, whole genome shotgun sequence.
ACCESSION   DBNGZE010000279 DBNGZE010000000
VERSION     DBNGZE010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1076402"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16FV_07230"
     CDS             complement(<1..245)
                     /locus_tag="AC16FV_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR2849884.1"
                     /translation="MTTGLRIHELTLEQQELWQRVNDLWALSLTRNATEIRNTIHPRY
                     VGWDMSSPVPHDREAAVQSVLGDSPAVTQYQLTPLSV"
     gene            complement(347..658)
                     /locus_tag="AC16FV_07235"
     CDS             complement(347..658)
                     /locus_tag="AC16FV_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849885.1"
                     /translation="MSKDLEAQFHQEMLNVYHRAKSECRYNAIRFLEMVTDHGGLAAA
                     KMLLASKHHPEGLTRLWKEGRLDISMEALVLKEPWRRLFSEEELRMARKRLDDLGYKG
                     G"
     gene            complement(655..1119)
                     /locus_tag="AC16FV_07240"
     CDS             complement(655..1119)
                     /locus_tag="AC16FV_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849886.1"
                     /translation="MSDADAPAMTSATAAIAERLRQLETELSRLIHSPVIRFDERCRS
                     ALPELPGIYRIFDLAIPDETVRAGRTTSAARGLRQRVYQNHLMGDQASNLRAQLVAGG
                     ECSDVEAAKRMIRERFGVQLLVVENSEERASLEHFMLAVLRPRYSDKRGLTA"
     gene            complement(1679..2962)
                     /locus_tag="AC16FV_07245"
     CDS             complement(1679..2962)
                     /locus_tag="AC16FV_07245"
                     /inference="COORDINATES: protein motif:HMM:NF023299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR2849887.1"
                     /translation="MRVSEKKKIDVMDLRWGMYIASLDRPWLETPFLFQGFVLQTPQE
                     MELLKKHCQHVYIDIIKGVDALPSQNRTEYESRMRAIQVEEEKIALKVRELADSPAMP
                     RQRPAYIDQVPLETEIVRAKEVEASARKTMREVIESIQKGKDFSIALVTKVVTDMVNS
                     ILRNPDALVCLTYLRNINEYTALHSIRTCILGIAFGRHLVFTREELIDLGVGFMLHDV
                     GMLKIPREIIEKPAGLNTQEFEIMNRHVGWGVALLNKNGGIPPLAMQVVQQHHERRDG
                     AGYPLRLKGDAIDQAGFIGSIVDVYDAVTSDRIYSGGLSAEDALKRMYEWREKDFHAE
                     LVEDFIRCMGIFPIGSLVELSTGSIGVVISINRARRLKPKVALVLTADKTPYSHKIVT
                     DLMEHTDGQGREIKISRVLPVGTHGIHPMDHIVQL"
     gene            complement(3077..>3281)
                     /locus_tag="AC16FV_07250"
     CDS             complement(3077..>3281)
                     /locus_tag="AC16FV_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849888.1"
                     /translation="TWGDSVMTDDEHSDKPAFNRPERRKKIRRTGKDRRDQVRWELEN
                     PTRRKGLGRRALDRLINAPDPKR"
BASE COUNT          689 a          928 c          923 g          741 t
ORIGIN      
        1 tcgacactca acggggtaag ttgatactga gtaactgctg gtgaatcccc cagcacggac
       61 tgaacggcag cttctctgtc atgaggtacc gggctactca tgtcccagcc gacatatcga
      121 ggatgaatcg tgtttcgaat ctctgtggca ttgcgcgtca gtgacaaagc ccacaagtca
      181 ttgactctct gccacagctc ctgctgctcc agggtgagtt cgtgaatgcg cagaccggtc
      241 gtcattgtgc tggctcggtt aggcccaacg tcgtggtgaa cggtgggcca ataagaacgg
      301 tgcctgaaaa agcgtggcgc ttgcggcccg tccgttccac ctagagttag ccacctttat
      361 acccgaggtc gtcaaggcgc tttcgagcca tacgaagttc ctcttcagag aacaggcgac
      421 gccacggctc cttcaacaca agtgcttcca ttgaaatgtc gaggcggcct tccttccata
      481 agcgcgtgag tccttccggg tggtgcttcg atgccaggag catcttggct gcggcaagtc
      541 cgccatgatc ggtcaccatc tcaaggaagc gaatggcgtt gtaccgacat tcacttttcg
      601 cccggtggta gacgttgagc atctcttgat gaaactgagc ctcaaggtct ttgctcatgc
      661 agtaagccca cgcttatctg agtaccgtgg ccgcaacact gcgagcatga aatgttcgag
      721 cgacgctctt tcctccgagt tttccacgac aagtaactgc acgccgaatc tctcccgaat
      781 catccgcttt gccgcttcaa catcggaaca ctcgccgcct gcaaccaatt gcgcgcgtag
      841 attgctggct tgatctccca tcaagtggtt ctgatataca cgttggcgca gcccgcgtgc
      901 tgcgctagtc gttcgaccgg ctcgaacagt ttcatccggt attgcgagat cgaatatccg
      961 ataaatgcca ggcaattcgg gcaaagcact ccggcatcgc tcatcgaacc taattactgg
     1021 cgaatggatg agtcgcgata attctgtctc aagctgccgc aggcgctcgg caatggccgc
     1081 cgtagcggac gtcatagccg gtgcgtcggc atcactcatc ggtcctccgg tggctaacag
     1141 ttgagtatac ggatcatgcg gcaatattct gccgcagtgt ccggtatatt gggaatgagt
     1201 tcattgaaaa gaaagaaaaa aatctgggta tatgagcaac tccttatagg ggatataccg
     1261 cacttgccgg tatgcactta gaccctgcaa ggctggctta gtgaaattca ggcagggcta
     1321 ggtgttgctc cagaccccgt agcgcgcacc ccgggtacga aggaaggttt cgcgcgctcc
     1381 ggggggagtg gttttggtga cttttgccga aacaaaagtc acccagggtg cggggcggag
     1441 caacccgcaa ttactaaagc cgccgcagcg gctccaatta gaacagcatc gccactggcg
     1501 atctgcgtgc ttttcacgca agatcatgat cccaaccacc aaggagcaag acgatgagca
     1561 agggcatgga cagtaagaaa cagaccaaga aggaaccggc caagaccatg aaggaaaaac
     1621 gcgcggcgaa gcgggccaag agggccgcgc ggggcggcat agggtaacaa cagcagggct
     1681 atagctgaac gatatggtcc atggggtgga tgccatgcgt ccccaccggg aggacgcgcg
     1741 agatcttgat ctcccgtccc tggccgtccg tgtgttccat caaatcggtg acgatcttgt
     1801 ggctgtaggg tgttttgtcg gcggtcagta cgagcgccac cttgggtttg agacgacggg
     1861 cccggttgat cgagatgacg acgccgatac tgccggtgct gagttccacc agggagccga
     1921 tggggaagat gcccatgcat cgaataaaat cctccaccag ctcggcgtga aaatccttct
     1981 cccgccattc gtacatccgt ttcagcgcgt cttcggctga cagaccgcca gagtagatgc
     2041 gatcgctggt cacggcgtcg tagacgtcga caatcgagcc gatgaatcct gcctggtcga
     2101 tcgcgtcccc cttcaggcga agcggatacc ccgcgccatc gcggcgctcg tggtgttgct
     2161 ggacaacctg catggcgagg ggagggatgc cgccattctt gttcaggagc gctacgcccc
     2221 agcctacgtg ccggttcatg atttcaaatt cctgcgtgtt caggccggcg ggcttttcga
     2281 tgatttccct gggaatcttc aacatgccga catcgtgcag catgaagccc acgcccaggt
     2341 cgatcaattc ctccctggtg aacaccagat gacgcccgaa cgcgatcccc aggatgcaag
     2401 tccggatgct gtgcagcgcg gtgtactcat ttatatttcg caggtaagtg aggcacacca
     2461 gggcatccgg attgcgcagg atgctgttca ccatgtcggt gacgaccttg gtaacgaggg
     2521 caatactgaa atctttgcct ttctggatac tttcgattac ctcccgcatg gtcttccggg
     2581 cgctggcttc gacctccttg gcgcggacaa tttccgtttc aagcggaacc tggtctatgt
     2641 aggccgggcg ctgacgcggc atggcgggag aatcggcgag ctcccttacc ttgagtgcga
     2701 ttttttcttc ttccacctgg atcgcacgca tgcgcgactc gtattcggtg cgattctgcg
     2761 agggtaaggc gtcgacaccc ttgatgatat cgatgtagac gtgttggcag tgtttcttga
     2821 gcagttccat ctcctgcggt gtttgcagta cgaacccctg gaacaggaaa ggggtttcca
     2881 gccagggccg atccagggag gcgatataca ttccccaccg caagtccatg acatctattt
     2941 tctttttttc agatacacgc atgcgattaa aacgctattt ctcttcagca ttatttcgac
     3001 acgatcttgc gtggaccgtc ttcgccacgc ccgccccccc atggcaatca acaccagcgc
     3061 gagagtcggc ggctgcttat cgcttgggat ccggcgcgtt aattaaccga tcgagggcgc
     3121 ggcgccccag gcccttgcgc cgcgtgggat tttccagctc ccagcgcacc tgatcgcggc
     3181 ggtccttgcc tgtgcggcgt atttttttac ggcgttcagg acgattaaat gcaggcttgt
     3241 cagaatgttc atcgtctgtc atgacgctgt ctccccacgt g
//
LOCUS       DBNGZE010000280         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1289770, whole genome shotgun sequence.
ACCESSION   DBNGZE010000280 DBNGZE010000000
VERSION     DBNGZE010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1289770"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1700
                     /locus_tag="AC16FV_07255"
     CDS             30..1700
                     /locus_tag="AC16FV_07255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2849889.1"
                     /translation="MESALPRVGQSQAEGRAAVEAQNWKLLKYFNFYRLAIALAASGI
                     AISVGKFSPFGEAHPGLFLSTSVVYAILSLTAAFSIHWEKPDYDSQATLLSFADVTLL
                     TIIMHASGGLASGLGLMLVVAIAGTSLMLGKRLTIFYASLAVIAVLLEHSWAWLTGAE
                     TTEVEMLQGFPQVGLFGVGLYATAFFGYLLATRLRATEELAQRRGVDVANLTQLNELV
                     IQRMQSGVVICDPQGNIRLINQSAQKYLGIHNETEKKSPLNEISPDLAIQLFQWLGNS
                     AVNRGRKVFTSRVGYSLLPRFVSLGDDKNAVKLIFLEDMSILKQQAQQLKMAALARLT
                     ASIAHEIRNPLGALTNAAQLLGETMNTENTEEKRLVKIIDEQSKRMNVIVQNVTQLSR
                     RDRINPVRLELEPWLEDFLRQYGDTVSVPRDAFVAHDIQGLSICVDPDQLYQVISNLC
                     QNALRHSPPFTGTPLIKFQGSRDGEDRPILDVIDWGAGVNPDIADNIFDPFFTTTPKG
                     TGLGLYIARELCEGNGAALNYHPGEGGVGSRFRISFVRAEDCAELGTL"
     gene            1697..>2004
                     /locus_tag="AC16FV_07260"
     CDS             1697..>2004
                     /locus_tag="AC16FV_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008078522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2849890.1"
                     /translation="MNKPKNQVLIVDDEPDIREVLELTLGRMNLETRSASNIEEARHL
                     LEDYKFDLCLTDMRLPDGNGIELVRHIQDKHPYLPVAVITAFGNMETAVAALKAGAFD
                     "
BASE COUNT          418 a          624 c          554 g          408 t
ORIGIN      
        1 ctgtgctatt tccatagggt atgacagcta tggaaagcgc ccttccccgc gtggggcaga
       61 gccaggcgga aggccgggcc gcggtagaag ctcagaactg gaagctcctt aaatacttca
      121 atttttatcg tctggcgatt gcgctggccg cctccggcat tgcgatttcc gtcggaaaat
      181 tctcgccttt cggcgaggcc catcccggcc tgtttctcag caccagcgtg gtttacgcca
      241 tcctcagtct gacagcggcc ttttccatcc attgggagaa acctgattac gatagccagg
      301 caacattgct ctccttcgcg gatgtcaccc tgctcacgat catcatgcac gccagcggcg
      361 ggttggccag cggcctggga ttgatgctgg tggtggccat cgccggcacc agcctgatgc
      421 tcggcaagcg cctgacaatc ttttacgcct cgctcgccgt tatcgccgta ctgctggaac
      481 actcgtgggc ctggctgacc ggcgcggaaa caaccgaagt cgagatgctg caaggattcc
      541 cgcaggtggg actgttcggg gtcgggctct atgccacggc cttcttcggt tatctgctcg
      601 ccacccggtt gcgcgccacc gaagaattgg cccagcgtcg cggcgtggat gtcgccaacc
      661 tcacgcaact caatgaactc gtcatccagc gcatgcaatc cggtgtcgtc atctgcgacc
      721 cgcagggaaa catacgcctg atcaaccagt cggcgcagaa atatctcggc attcacaacg
      781 aaaccgagaa gaaatccccg ctcaatgaaa tctcgcccga tctcgccatc cagctgttcc
      841 aatggctggg caacagcgcc gtcaatcggg gacgcaaggt attcacctcc cgcgtgggtt
      901 attccctgtt gccccgtttt gtctcgctgg gtgatgacaa aaatgcggtg aagctgattt
      961 ttctggaaga catgtcaatc ctgaaacaac aggcgcaaca gctgaaaatg gcggcgctgg
     1021 cgcggttgac cgccagcatc gcgcacgaaa tccgcaaccc ccttggcgcg ctcaccaatg
     1081 ccgcacaact tctcggcgaa accatgaaca ccgaaaacac cgaggagaag cgtctcgtca
     1141 agattatcga tgagcaaagc aagcgcatga acgtcattgt gcagaacgtc acgcaactca
     1201 gccggcgcga ccgcatcaac ccggtccggc tggaactcga gccgtggctg gaggattttc
     1261 tgcgccagta tggcgacact gtttccgtcc cgcgtgacgc cttcgtcgca cacgacattc
     1321 aaggtctgtc catatgcgtg gacccggatc agctctatca ggtcatttcc aatctgtgcc
     1381 aaaacgccct gcgtcatagc ccgcccttta ccggcacgcc tctgatcaag ttccagggca
     1441 gtcgcgacgg cgaagaccgt cccatcctgg atgtcattga ctggggtgcc ggcgtcaatc
     1501 ccgacatcgc ggacaatatt tttgatcctt tcttcacgac cacgccgaaa ggaacagggc
     1561 ttggcctgta tatcgcgcgc gaattgtgcg aaggcaacgg cgccgcgctc aattaccacc
     1621 ccggggaagg cggggttggc agccgcttcc ggatttcatt cgtgcgcgcc gaagactgcg
     1681 ccgagctggg gacgctatga acaaaccgaa gaaccaggtt ctgattgtcg acgacgagcc
     1741 ggacattcgc gaggtcctcg agctgacgct gggccgcatg aatctcgaga cgcgttccgc
     1801 ctcgaatatc gaggaggcgc gccatctgct cgaggattac aaattcgatc tatgcctgac
     1861 tgacatgcgc ctgccggacg gtaatggcat cgaactcgtg cgtcacatcc aggacaagca
     1921 tccttacctg ccggtggcgg tcatcaccgc cttcggtaac atggaaactg ccgtggccgc
     1981 cctcaaggcc ggcgccttcg actt
//
LOCUS       DBNGZE010000281         5242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_109459, whole genome shotgun sequence.
ACCESSION   DBNGZE010000281 DBNGZE010000000
VERSION     DBNGZE010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_109459"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1779)
                     /locus_tag="AC16FV_07265"
     CDS             complement(19..1779)
                     /locus_tag="AC16FV_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR2849891.1"
                     /translation="MTLILTVIATSVATFLLTIWYWRDGGRHSSVEKWSASTEKEQLQ
                     AIMNNVTDGVVAIDARGVIGMFNPVATHIFGYSHAEVQGMNVDRVLTDIRTGGNGTIS
                     TGTREVVGIRKNGATFQADMTLCESGDRSNPGFIAIIRDITERKQAEQRLVYLAQYDA
                     LTGLPTRMLFHDRATQALARADREEKLVAIMYLDLDHFKHINDSMGHHAGDELLKAVA
                     KRVTSCLRSVDTVSRMGGDEFTVILESIPHIDNATIVAEKILDVMQEPFHLDGHDVHV
                     TVSIGITLYPMDDKDLTSLVKDADQAMYRAKQAGRNNYQLFGSDMAENAVAVKQMESQ
                     IGNALERGELCLHYQPRVDAVSGSITGVEALLRWQHPEMGLISAKDFMPLLERSRLVY
                     SVDEWVLRTACLQNHQWQKIGLPPVSVTVNISPYRFRRKGLAELVEKVLKETKLPPNY
                     LELDVPPENLVGVAHENYRSILNQLSALGVHVALVDPAVGGSILDSFQKLPQYVMKID
                     RSFVRDATVDSDHAAIVQAVVRLARTMKLKVVAEGVETREQLDALRDYGCDMVQGYLF
                     SKPVPTEDITRMLREGKRLV"
     gene            1967..2659
                     /locus_tag="AC16FV_07270"
     CDS             1967..2659
                     /locus_tag="AC16FV_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR2849892.1"
                     /translation="MEREYLTILIHLGAAVLAGGLIGLERAYHGRPAGFRTHTLVCTA
                     SSLLMLLTVYQWQLLAGVPLETVRVDPTRMAQGIMTGIGFLGAGVIMKDGLTVRGLTT
                     AASIWMTASIGILIGIGFYFPAIVATLLTLGTLSLFRWVEAIMPSQYYARLHVRFKRQ
                     ELTSEPELRTLITGHGFSVANLSYQLADEGKVFEYQMTIRTDDRDSYRRLAETLTAKE
                     QVLEFHIYPTGD"
     gene            complement(2673..3170)
                     /locus_tag="AC16FV_07275"
     CDS             complement(2673..3170)
                     /locus_tag="AC16FV_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR2849893.1"
                     /translation="MGILRVIRHLFMPPWRVRAVFSARTLRAIEAAIRAAESTHAGEI
                     RFAVESALPLIPLLRGQSARHRALEVFSQLRVWDTEHNNGVLIYLLLADRDLEIVADR
                     GVHMRVGSEGWEKICREVEARFRESKFEEGVLHGIRAVSVHLATHYPAQGKDRNELDN
                     RPVVL"
     gene            complement(3170..4039)
                     /locus_tag="AC16FV_07280"
     CDS             complement(3170..4039)
                     /locus_tag="AC16FV_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR2849894.1"
                     /translation="MMSAKIRGLARGLLLLAFFLASFAIRAEVAVPPLTARVTDLTAT
                     LTPSQRDTLERELRAFEARKGSQIAVLIVPTTQPEVIEQYSLRVAETWKLGRKGVDDG
                     VLLLIAKDDRELRIEVGYGLEGVIPDAVAKRIISEIIIPYFKQGDFYGGIQAGVGRLI
                     RLVDGEPLPPPQARDNSWSGIFDYAPFIIIAAFVGGGILRALFGRLIGGSVAGGVTGL
                     ILWFIAGSALMALIAGIVVFVLVLAAGVQLGSSRYGGWPGGGYGGDWSSGGGGGFSGG
                     GGGFGGGGASGKW"
     gene            complement(4061..4687)
                     /locus_tag="AC16FV_07285"
     CDS             complement(4061..4687)
                     /locus_tag="AC16FV_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR2849895.1"
                     /translation="MRNKIILPLLLVVYSMLGGCGYNTLQTTDEQIKAGWAEVLNQYQ
                     RRADLVPNLVNVVKGYAAHEKEVLEQVTNARAKVGSIQVTPELVNDEQALAKFQAAQG
                     ELSSALSRLLAVAENYPQLKADGLFRDLQAQLEGTENRITVARNRYIQAVQAYNVTVR
                     QFPTNLTAMLFGYKVKPSFTVENEKEIAKPPKVDFGGAPPSPTGAGKQ"
     gene            complement(4717..>5242)
                     /locus_tag="AC16FV_07290"
     CDS             complement(4717..>5242)
                     /locus_tag="AC16FV_07290"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR2849896.1"
                     /translation="AEKYSHLVAKFAEQYKVSPSLVYAVIRTESNFNPFAVSSAPAYG
                     LMQLVPGSGGREAYRRAKGQDETPSKQYLFDAGNNIELGAAYLNVLTYSQLEPVANSV
                     SREYCVISAYNTGPSNVLRTFSKDKVAAVNAINSLAPSAVYQKLRSHLPYEETRQYLY
                     KVVNYRKQFVTKVN"
BASE COUNT         1019 a         1634 c         1481 g         1108 t
ORIGIN      
        1 cgcggcgcgt cattgcgtct aaacgagccg cttcccttcg cgcagcatgc gggtaatgtc
       61 ctcggtcggc acgggtttgc tgaacagata gccctgcacc atgtcgcagc cgtaatcccg
      121 cagcgcgtcc agctgttccc gggtttccac gccctcggca acgaccttca acttcatggt
      181 acgggccagt ctgaccaccg cctgcacgat cgcggcatga tcgctgtcca ccgtggcgtc
      241 acgcacaaag gaacggtcga ttttcatcac atactgcgga agcttctgga aggaatcgag
      301 aatgcttccg ccaacggctg ggtccaccag cgccacgtgc acgcccaagg cactcaattg
      361 attcaaaata gaacggtaat tctcgtgcgc cacgcccacc agattttccg gggggacatc
      421 gagctccaga taattcggcg gcagcttcgt ttccttcagg actttttcga ccagctccgc
      481 aagacccttg cggcggaagc gatagggcga aatattgacc gtgacgctca cgggcggcaa
      541 accgatcttc tgccactgat gattctgcaa acaagccgtg cgcagcaccc attcatccac
      601 gctgtagaca agcctggatc gttccagcaa tggcatgaaa tccttggcgg agatgagccc
      661 catctccgga tgctgccagc gtaacaaggc ttccacgccc gtaatacttc cgctcacggc
      721 atccacgcgt ggctgatagt gcaggcacag ttcaccgcgc tccagcgcgt ttccgatctg
      781 tgactccatt tgcttcaccg ccaccgcgtt ttccgccatg tcgctaccga aaagttgata
      841 gttgttccgg cccgcctgtt tggcgcggta catggcttgg tcggcatcct tgacgaggct
      901 ggtcagatcc ttgtcatcca tgggatagag cgtgatgccg atgctgaccg tgacatgcac
      961 atcatgtccg tccagatgaa atggctcctg catgacatcg aggatcttct cggccacgat
     1021 cgtggcgttg tcgatgtgcg ggatgctctc cagaatcacg gtgaattcat cgcctcccat
     1081 gcgcgacacc gtatccacgc tccgcagaca ggaagttact ctcttcgcca ccgctttcag
     1141 cagttcatcg ccggcgtgat ggcccatgct gtcattgatg tgcttgaagt ggtccagatc
     1201 cagatacatg atcgccacga gtttctcctc gcggtcggct cgcgccagcg cctgcgttgc
     1261 gcgatcatgg aacagcatgc gtgtcggcag gccggtcagg gcatcgtatt gcgcgaggta
     1321 aaccaggcgt tgttccgcct gtttgcgttc ggtgatatcg cggatgatgg cgatgaatcc
     1381 gggattgctt ctgtcgccgg attcgcacag ggtcatatcc gcctggaacg tcgctccgtt
     1441 tttgcggatg cccaccactt cacgcgtgcc ggtactgatg gttccgttcc ctccggttcg
     1501 gatatccgtc aacaccctgt ccacattcat tccctgaacc tcggcatggg aatatccgaa
     1561 aatgtgcgtc gcgacgggat tgaacatgcc gatgacgcct cgcgcatcga tggcgaccac
     1621 gccatcggta acgttgttca tgatggcctg taattgctcc ttttccgtgg aggcgctcca
     1681 tttttcgaca gaggaatgac gaccgccgtc gcgccaatac catatcgtca ggagaaacgt
     1741 cgccaccgag gtggcgatta ccgtcagaat cagggtcatg acgtcaaatg gacatgatga
     1801 atgagggcca aacttcccga tgagttcata tgcctgcaac attatttttg ggcttgtcaa
     1861 ttccaagaat agactgcccg attgtgaatg ccattctgcg caacccccgt cctttcctgc
     1921 cgccagcccg tctggcgcgc gcgcggttta gggctaaact gcgcttgtgg aaagggaata
     1981 tctcaccatc ctgatacatc tcggcgcagc ggtgctggcg ggcggcctga tcggcctgga
     2041 acgcgcctat cacgggcgtc ccgccggctt tcgtacccat accctggtct gcacggcctc
     2101 cagtctgttg atgctgctga cggtttatca gtggcagttg ctggccggtg tgccgctgga
     2161 aacggtgcga gtagacccca cgcgcatggc gcagggcatc atgaccggca tcggcttcct
     2221 cggcgccggt gtgatcatga aagacggtct caccgtgcgc ggcctcacga cagcggcgtc
     2281 catctggatg accgcttcca tcggcatact gatcggtatc ggcttttact ttcccgccat
     2341 tgtcgcgacc ctgctcacgc tcggcacctt gtcgctgttt cgctgggttg aggccatcat
     2401 gccctcacag tactacgcgc gactgcatgt acgcttcaaa cgccaggaac tgacgtctga
     2461 acctgagctg cgcaccctga ttacgggaca tggtttctcg gtggccaacc tgagttacca
     2521 attggcggat gaggggaagg ttttcgaata ccagatgacg atacgcaccg acgatcggga
     2581 cagctaccgg cgcctcgccg aaacgcttac cgccaaagag caggtgctgg agtttcacat
     2641 ctatcccacg ggcgactgag tcgtccgcgc acctagagca cgaccggcct attgtcgagc
     2701 tcgttgcggt cttttccctg cgcgggataa tgtgtggcca gatgcacgct gacggcgcga
     2761 ataccatgca gcacgccttc ctcgaactta ctctcgcgaa accgtgcttc cacctcgcgg
     2821 cagatttttt cccaaccttc gctgcccacg cgcatgtgca cgccacggtc ggcgacgatc
     2881 tcaaggtcac gatccgccag cagcagataa atcaacacgc cgttgttgtg ctcggtgtcc
     2941 cacacacgca gctgcgaaaa tacctcgagc gcccgatggc gcgccgactg tccgcgtaac
     3001 aatggaatca atggcagtgc gctctccacg gcaaagcgga tttcgcctgc gtgagtggac
     3061 tcggccgcgc ggatggcggc ctcgatggcg cgcagcgtgc gcgccgaaaa caccgcacgc
     3121 acgcgccacg gtggcatgaa cagatgtcga atcacgcgca gaatacccat caccatttcc
     3181 ccgaggcgcc gccgcctccg aatccaccac ctccgccgct gaaacctcct ccaccaccgc
     3241 tgctccagtc gccgccataa ccgccgccag gccaaccgcc gtaacgcgag gaaccgagtt
     3301 gcacgcccgc ggccagcacc agcacgaaca cgacgatgcc ggcgatcagt gccatcaacg
     3361 cggaaccggc aatgaaccag aggatcagcc cggtgacgcc gccggccacg gacccgccga
     3421 tcagccggcc gaacaacgcg cgtaggatgc ccccgccgac aaacgccgcg atgatgatga
     3481 acggcgcata gtcgaagatg ccggaccagg aattgtcccg cgcctgcggc ggtggcaggg
     3541 gttcgccgtc gacgaggcgg atcaggcggc cgaccccggc ttgaatacca ccgtagaagt
     3601 cgccctgttt gaagtaagga atgatgattt cgctgataat gcgtttggcc acggcgtcgg
     3661 gtatcacacc ttcgagaccg taaccgacct cgatgcgcag ttcgcggtcg tccttggcaa
     3721 tgagcaacag cacaccgtca tccacaccct tgcgtccgag tttccaggtt tcagccacgc
     3781 gcagggaata ctgttcgatg acctcgggtt gcgtcgtggg cacgatcaac accgcgatct
     3841 ggctgccctt gcgcgcctcg aaggcgcgca gttcccgttc gagcgtgtcg cgctgggacg
     3901 gcgtgagcgt ggcagtcagg tcggtgacgc gtgccgtcag cggcggcact gccacttccg
     3961 cgcgaatggc aaaactggcg aggaaaaacg ccaggaggag cagtccgcgc gcgagccccc
     4021 tgatcttggc gctcatcatg ccgccgtcaa tagcctggcg ttattgtttt ccggcgccgg
     4081 taggcgaagg cggcgcgccg ccgaaatcga ccttcggtgg cttggcgatt tccttttcat
     4141 tctccacggt gaagctgggt ttcaccttgt agccgaaaag catcgccgtc aggttggtcg
     4201 ggaattggcg cacggtgacg ttgtacgcct gtaccgcctg gatgtagcgg ttgcgcgcca
     4261 cggtgatgcg gttctccgtg ccttcgagtt gcgcttgcaa atcgcggaac aggccatcgg
     4321 ccttgagctg cggataattc tccgccactg caagcaggcg cgacagggcg gaactcagtt
     4381 cgccttgcgc ggcctggaat ttggccagcg cttgttcgtc atttaccagc tccggggtaa
     4441 cctggatcga acccactttg gcgcgcgcat tcgttacttg ttccagcact tccttttcgt
     4501 gcgccgcata acccttgacc acgttcacca gattcggcac cagatcggcg cggcgctgat
     4561 actggttcag cacctcggcc cagcccgcct tgatctgttc gtcggtggtt tgcaacgtgt
     4621 tatagccaca gccgccgagc atgctataga cgaccagcaa caatggaaga atgattttgt
     4681 tacgcatggc tctctccttg atgcacgaca aaatgttcaa ttcaccttgg tgacaaattg
     4741 cttgcggtaa ttgaccacct tatataggta ttgccgcgtt tcctcgtacg gaagatgcga
     4801 gcgcaatttc tggtacaccg cggacggcgc aaggctgttg atggcattca ctgcggcgac
     4861 cttgtccttc gagaacgtcc gcaatacatt gctcggcccg gtgttgtagg ccgagatcac
     4921 acaatattcc cgcgacaccg aattagctac gggctccagc tggctgtagg tgaggacatt
     4981 cagataagcc gcgccgagtt cgatattgtt cccggcgtcg aacaggtatt gcttggaagg
     5041 ggtttcatcc tgacccttgg cacggcgata ggcctcgcgc ccgccgctgc cgggcaccag
     5101 ctgcatcaat ccatacgccg gcgcggaact gacggcaaag ggattgaaat tgctttcagt
     5161 gcggataacg gcatacacca ggctcgggct gaccttgtat tgctcggcga atttcgccac
     5221 cagatggctg tatttctcgg cc
//
LOCUS       DBNGZE010000282         3924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_456237, whole genome shotgun sequence.
ACCESSION   DBNGZE010000282 DBNGZE010000000
VERSION     DBNGZE010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_456237"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1252
                     /locus_tag="AC16FV_07295"
     CDS             152..1252
                     /locus_tag="AC16FV_07295"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2849897.1"
                     /translation="MNPIVLLLDDSRENHVLLPRMLKGLPGMEAIQTVCFASGGDALD
                     WCRENEPDLCVVDYQMPGMDGITFIAEARKLPRFGGIPMIMITGAADTELRHRALMNG
                     ANDFLTRPVDAEEFKARVYSLLSLRLSLVNPLDRVERLAHDVELLERQALEREHEQII
                     FKLSQLSTSRDEETGNHMHRVAHISRLIAHELGHDEQFCDMIYLAAPMHDIGKVGIPD
                     KILLKPGKLTPDEWEIMKTHTTVGYDVLKDSSSPLLRMGADIAHSHHEKYNGQGYPQG
                     LVGEAIPVVGRIVAVADELDALLSVRPYKHAWNLKDALEQMRRERGRHFDPACVDIML
                     RHIDAIVDIQKRFADVSEVVLPEALPLRRGVA"
     gene            complement(1273..2088)
                     /locus_tag="AC16FV_07300"
     CDS             complement(1273..2088)
                     /locus_tag="AC16FV_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR2849898.1"
                     /translation="MVEIVPKIQALFLLPPGIIIVIALLGFLIQIRWHLLGSFIVFIS
                     IAGLLVLSLPLTGYELMRGLESRIPPLRLTAAADAGARPGAIVILGGGRYTEAPEYGD
                     GDSVNHATLERLRYGAHLHRLTGLPILVSGGAPYAEKISEAELMQASLVNDFRVEVKW
                     VEKKSATTHENAKYTKMILAEAGVRRVYLVTHAAHMPRALWSFEIEGISAVPAPMGFS
                     TLNKEDRETLGYFPSAYGLRLSSNALRERLGLFWYKRKYGSDPAPETAPAPAR"
     gene            complement(2091..3047)
                     /gene="lipA"
                     /locus_tag="AC16FV_07305"
     CDS             complement(2091..3047)
                     /gene="lipA"
                     /locus_tag="AC16FV_07305"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010446712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR2849899.1"
                     /translation="MDKDKLPSHAPIDQKGAAKTARIPIKIAPQAELPRKPAWIRAKS
                     PTHLEVGRLKNLLRTHALHTVCEEASCPNLGECFSHGTATFMIMGRLCTRRCPFCDVA
                     HGRPLPLDADEPENLARAIAEMKLKFVVITSVDRDDLRDGGAAHFSACIQAVRRHTPA
                     TSIEVLVPDFRGRMEVALEALGAALPDVFNHNLETVPRLYKAARPGADYRHSLELLRR
                     FRERHSGIPTKSGLMVGLGETNEEILETMRDLRDHGCELLTVGQYLQPSRHHLPVTRF
                     VTPAEFDNFAQAARAMGFRNVASGPMVRSSYHAEQQAAGRSF"
     gene            complement(3064..3735)
                     /gene="lipB"
                     /locus_tag="AC16FV_07310"
     CDS             complement(3064..3735)
                     /gene="lipB"
                     /locus_tag="AC16FV_07310"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR2849900.1"
                     /translation="MRAARHATPADDVTLRSLGCVDYSATFRAMQDFTQARTPQTPDE
                     IWLLEHPPVFTMGLKGRGGTRTHIGDIPLAYTDRGGDITYHGPGQLVAYILMDLQRRG
                     WGPKRLVTALEQAVIELLADHGIRGERRVGAPGVYAAQGKIASLGLRVKQGRSYHGLA
                     LNINMDLTPFSRIDPCGYPGQAVTQLADLGVRADLTTIGRELAGKLTGELGYTDCHRS
                     LDKPR"
     gene            complement(3735..>3924)
                     /locus_tag="AC16FV_07315"
     CDS             complement(3735..>3924)
                     /locus_tag="AC16FV_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HIR2849901.1"
                     /translation="PVKTEILSAADEVWLTSSTKEILPITRIDGRPVGNGKPGPMHAK
                     MFALYKEYKQAFIQGQVD"
BASE COUNT          713 a         1205 c         1256 g          750 t
ORIGIN      
        1 gtctcaatcc aagtgtcggg gttcaagaat gccgtaaaat cgcaggtagc gacgtatccg
       61 gtcttctgat tcaacgctcc gttgagaggt cgcctctggc acggaacttg taccgttttc
      121 ccgcataaca tccgcatttt ggggtgcccg tatgaatccg atcgttctcc tattggacga
      181 cagcagggag aatcatgtgt tactgccacg gatgctcaag ggactgcccg gaatggaagc
      241 cattcaaacc gtgtgtttcg ccagcggcgg cgacgcgctc gactggtgtc gtgaaaacga
      301 acccgacctg tgcgtggtgg actaccagat gccgggcatg gacggcatca cgttcatcgc
      361 cgaagcccgc aagcttcccc gcttcggcgg cattcccatg atcatgatta ccggcgccgc
      421 cgacaccgag ctgcggcacc gcgccctcat gaatggcgcc aacgattttc tgacgcgacc
      481 ggtcgacgcg gaagaattca aggcgcgcgt ctacagcctg ttgtcgctgc gcctgagtct
      541 ggtcaatcca ctcgaccgcg tggagcggct ggcgcatgac gtggagctcc tcgaacgcca
      601 ggcgctcgag cgcgaacacg aacagattat tttcaaactc tcccagctca gcacctcgcg
      661 cgacgaggag accggcaacc atatgcatcg ggtggcgcat atctcgcgcc tcatcgcgca
      721 cgagctggga cacgacgagc agttctgcga catgatctat ctggcggcgc cgatgcatga
      781 catcggcaag gtcggcatcc cggacaagat actgctcaag cccggcaagc tcacgcccga
      841 cgaatgggag atcatgaaga cgcataccac cgtcggctat gacgtcctca aggattccag
      901 ctcgccgctg ctgcgcatgg gcgcggatat cgcgcactcg catcacgaga aatacaacgg
      961 tcagggttac ccgcagggac tggtgggcga ggcgataccg gtggtgggac gcatcgtggc
     1021 ggtggccgac gaactggatg ccctgctgtc agtgcgcccg tacaagcatg cctggaatct
     1081 caaggacgcg ctggagcaga tgcggcgtga gcgcgggcgg catttcgatc ccgcctgcgt
     1141 cgatatcatg ctgcggcaca tcgacgcgat tgtggatatc cagaaaagat tcgccgacgt
     1201 atccgaagtt gtgcttcccg aggcgctacc cctgcgacgt ggagtagcgt agttcgccga
     1261 gaaccccctg gatcagcgcg cgggagcggg tgctgtctcc ggcgctgggt ctgatccata
     1321 cttgcgcttg taccaaaaaa gccccagacg ctcgcgcagc gcgttgctgc tcaagcgcag
     1381 gccgtaggcg gacggaaaat atcccagcgt ttcacggtct tccttgttga gcgtggagaa
     1441 acccatgggc gccggcacgg cgctgatgcc ctctatttcg aacgaccaca gggcgcgtgg
     1501 catgtgtgcg gcgtgggtga cgaggtagac acgccgcact cccgcctccg ccaatatcat
     1561 cttcgtgtat ttggcgtttt catgggtagt ggcggatttt ttttccaccc atttgacctc
     1621 gacccggaag tcgttcacca acgaggcctg catcagctcc gcttcggaga ttttttccgc
     1681 atagggcgcg ccgccgctga ccaggattgg caggcccgtg aggcgatgca aatgcgcgcc
     1741 gtaacgcagg cgttccagtg tcgcatgatt tacgctgtcg ccatcgccgt attccggcgc
     1801 ctcggtgtaa cgaccacccc ccaggatcac aatggcgccg ggccgcgcgc cggcgtcagc
     1861 ggccgcggtc aaccgcagcg gcgggatgcg tgattcaagt ccgcgcatca gctcgtagcc
     1921 ggtcagcggc aggctcagga caagcagccc ggcgatgctt atgaaaacga tgaaactgcc
     1981 aagcaagtgc cagcggatct ggatgagaaa tccgagcagg gcgatgacga tgatgatgcc
     2041 cgggggcagc aggaatagcg cctggatttt ggggacgatc tcaaccacgg tcagaaggag
     2101 cgtccggccg cctgctgttc agcgtgatag gaagagcgca ccatcggacc gctcgcgacg
     2161 ttgcggaagc ccatggcgcg cgcggcttgg gcaaagttgt cgaattccgc cggcgtcacg
     2221 aagcgcgtca ccggcagatg atgccggctc ggctgcaggt actggccgac cgtgagcagc
     2281 tcgcagccgt ggtcgcgcag atcgcgcatc gtttccagaa tttcctcgtt ggtttcaccg
     2341 aggcccacca tcaggccaga cttggtcggg atgccggagt ggcgttcccg gaagcggcgc
     2401 agcaattcca acgagtgccg gtagtcggcg cccgggcgcg cagccttgta taggcgcggc
     2461 acggtttcca ggttgtgatt gaacacgtcc ggcagcgccg cgcccagcgc ctcgagtgcc
     2521 acttccatgc gtccgcggaa atccggtacc agaacctcaa tcgaagtggc gggcgtgtgc
     2581 cggcgcaccg cctggatgca ggcgctgaag tgcgccgcgc cgccgtcgcg caggtcgtcg
     2641 cgatccacgg aggtgatgac cacgaacttc agtttcatct cggcaatcgc gcgcgcgaga
     2701 ttttccggtt cgtcggcatc gagcggcagc ggccggccgt gcgccacgtc gcagaacggg
     2761 cagcggcgcg tgcacaaccg gcccatgatc atgaaggtgg cggtgccgtg gctgaagcat
     2821 tcgccgagat tcgggcacga ggcctcctcg cacaccgtgt gcagcgcatg cgtgcgcagc
     2881 agattcttca ggcggccgac ctcgagatgt gtcggacttt tggcgcggat ccacgccggt
     2941 ttgcgcggca gttccgcctg cggcgcgatc ttgatgggaa tgcgcgcggt cttggccgca
     3001 cccttttggt cgatgggcgc gtgtgacggc aatttgtcct tgtccacgac gtttacaaag
     3061 gttttagcgg ggtttgtcca gtgaccgatg gcagtcagta taacccagct cgccggtcag
     3121 tttcccggcc agctcccggc cgatggtcgt cagatcggca cgcacgccca gatcggccag
     3181 ttgcgtgacc gcttgccccg gataaccgca gggatcgata cgggaaaaag gcgtgagatc
     3241 catgtttata ttgagcgcca gtccgtgata actgcgtccc tgtttcacgc gcaagccgag
     3301 cgaggcgatc ttgccctgcg ccgcgtagac accgggcgcg cccacgcgtc gctcgccgcg
     3361 gatgccgtgg tcggccagca gctcgatgac cgcctgttcc agcgcggtga ccaggcgttt
     3421 cggaccccag ccgcgacgtt gcaggtccat gagtatgtag gccacgagct gtcccggtcc
     3481 gtgataggtg atgtcgccgc cgcggtcggt gtacgccagc ggaatgtcgc cgatatgcgt
     3541 gcgtgtgccg ccgcggccct tgagccccat ggtgaacacc ggtggatgtt cgagcagcca
     3601 gatctcatcc ggtgtttgcg gcgtgcgtgc ctgggtaaag tcctgcatgg cgcggaacgt
     3661 ggcggaatag tccacgcatc cgaggctgcg cagcgtcacg tcgtcggccg gagtcgcgtg
     3721 gcgcgccgcg cgcatcaatc cacctgcccc tgaatgaagg cctgtttgta ttctttataa
     3781 agcgcgaaca ttttggcgtg catcggtccc ggtttgccgt tgcctaccgg tcggccatcg
     3841 atgcgcgtga tcggcaggat ttccttggtc gagctggtca gccacacctc gtccgcggcg
     3901 gatagtatct cggttttcac cggc
//
LOCUS       DBNGZE010000283         3912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1707043, whole genome shotgun sequence.
ACCESSION   DBNGZE010000283 DBNGZE010000000
VERSION     DBNGZE010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1707043"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /gene="rsmI"
                     /locus_tag="AC16FV_07320"
     CDS             <1..766
                     /gene="rsmI"
                     /locus_tag="AC16FV_07320"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR2849902.1"
                     /translation="EADLIAAEDTRHSGKLLKHFGIGTKTEAIHEHNERSQVPRLVEI
                     LKGGKSIAFITDAGTPLVSDPGFHLVRAARQAGLRVVPEPGACAAIAALSAAGLPSDR
                     FVFEGFPPAKSAARLAVFEKLREESRTLIFYESPHRILESLKDMSEVFGPGREAVLAR
                     ELTKQFETLHADTLQELLEWVGRDANQQLGEFVILIHGVPRAEQEAMDEEAKRVLKIL
                     LDELPVSQASALAARITGLKKNKLYEYALSLKGGSE"
     gene            1229..3850
                     /locus_tag="AC16FV_07325"
     CDS             1229..3850
                     /locus_tag="AC16FV_07325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIR2849903.1"
                     /translation="MSKKKSNTKKPRKEVRKAKGGSQDLSERGRERGNPGQHVLPDDD
                     FSLESQTAPSEEAAKPHAGRNKSATPAGKKSGHPEVLSYRHQDKRKNNPEVGMVKPEN
                     DPDQPRTVWNYDPHLDPALQFDTGRAAIEKLIDEALASGDDAAMRAALQELKRLGEPY
                     LNWTGKAERTSFEVDTVSLHVHERIDPASILSAIRKNVGRISPSGRNPPNAYMHQPDL
                     FAAPFENLPLRDAIDFYKHERGWANRLIAGDSLLVMNSLLQKESLAGQVQMIYIDPPY
                     GIKYGSNFQPFVNKRDVKDRKDEDLTQEPEMIKAFRDTWELGIHSYLTYLRDRLLLAK
                     DLLHESGSVFVQISDENLHHATELCDEIFGPQNRISIIAFKKTNRALGSKYVGGVTDY
                     IIWYAKDELKLKFRTLFELKEAGEVGGTNYQFAELTSGQRVRTSAIDEGEIKDVRIFS
                     PVDLMASFYTASCDYEINFQGRSFTPRPGNSWATHPAGTQRLAKSARMMMVGERPYYV
                     AYLLDYPVLPLSNLWTDTRGEMSKIYTVQTTQRVIERCLLMTTDPGDLVLDPTCGSGT
                     TAYVAEKWGRRWITTDTSRVAVTLAKQRLMTASYDYYALRYPQEGLKGGFIYKTVPHV
                     TLKSIANNPEIDEIHARMYPAVEVALDQLNEALGRVRPTHQKRGAQGAPYMNEWEVPF
                     DFPEDWPQSARKPFEAFHAARQAMQKQMDASIAAHADQETLYDQPQPDKNKLRITGPF
                     TVEAVPFPTVLALDEAKPAQEADVAIARSGESWRQHQWRDELLKAGIRGKGGQMLKFA
                     ELETFPASEHIRHLHCSGTLAETGERVVVSFGPEHAALEQRQVKLALDEAEKLRPAPK
                     FIIFASFEFDPEAGQGH"
BASE COUNT          912 a         1159 c         1155 g          686 t
ORIGIN      
        1 ggaggccgat ctgatcgccg ccgaggacac gcgccacagc ggcaaactgc tgaagcattt
       61 cggtattggc accaagacgg aagccatcca tgagcataac gagcgcagcc aggttcccag
      121 acttgtcgaa atcctgaagg gcggtaagtc tatcgcattt attaccgacg cgggtacccc
      181 gctcgtgagc gaccccggct tccatctggt gcgcgccgcc cgacaggcgg gcctccgtgt
      241 ggtgcccgag cccggcgcct gcgccgccat cgccgcgttg tcggccgccg gtttgccttc
      301 cgaccgtttc gttttcgaag ggttcccgcc ggccaaatcg gccgcgcgcc tggccgtgtt
      361 cgagaagttg cgcgaggaaa gccgcacact catcttttat gaaagcccgc atcgcattct
      421 ggaaagcctg aaggacatga gcgaggtttt cggccccggg cgcgaggcgg tgctcgcgcg
      481 cgaactcacc aagcagttcg aaaccctcca cgccgacact ctccaggagc ttctcgaatg
      541 ggtcggccgt gacgcgaacc agcagctcgg cgagtttgtc atcctcatcc acggcgtgcc
      601 gcgtgcggag caggaggcca tggacgaaga ggccaaacgc gtcctcaaga tcctgctcga
      661 cgaactgccc gtgagccagg cgtctgcgct cgccgcaaga atcaccggcc tcaagaaaaa
      721 caagctttac gaatatgcct tgagccttaa gggcggcagt gaatagatca aacggcccgg
      781 agaatgacgg caaggacaga cccaacacta tatttaatgt agggcgggtg gagcgaagcg
      841 acaaaatcgc cgggagcgat tttggacagc cgaagactgg cccgcagggc ggagtccagg
      901 gatggagtcc gccaacccgc cgaacaatat aaggaacctc tgattaattc gtcattccgg
      961 ccaagggccg caggcccgcg agccggaatc caggaaaatc atatattaac tggatgccgg
     1021 ctttcgccgg catgacgaaa accgaattaa tcagaccttt cataatatga ttttgttggg
     1081 tccgccgctg gcgcggcttg acccaaccta catgagaagc gatgtgtggg cgctccctta
     1141 tgttaggatt cccgcaatga ataaagggat tgaggagatt cacctgtacc attagcgcgt
     1201 tgccaagaca ggcaacagag acttactcat gtcaaaaaag aaatcgaata caaagaaacc
     1261 gcgcaaggaa gtcaggaaag caaagggagg ctcgcaggac ctgtccgaac gcggccggga
     1321 gcgcggtaac cccggccagc atgtgctgcc ggacgacgac ttctcgctgg aatcgcagac
     1381 ggcgccaagc gaagaggccg ccaaaccgca cgcgggcaga aacaaaagcg cgacgcccgc
     1441 cggaaagaaa agcggacatc ccgaagtgct gagctaccgc caccaggaca agcgcaagaa
     1501 caacccggaa gtgggcatgg tcaagcccga gaacgatccg gatcagccca ggaccgtctg
     1561 gaactacgac ccgcatctcg acccggcgct gcaattcgac accggccgcg cggcgatcga
     1621 aaagctcatc gacgaggcgc tggcgagcgg cgatgacgcg gccatgcgcg ccgcgcttca
     1681 ggaactcaag cgcctcggcg aaccttatct caactggacc ggcaaggccg agcgcaccag
     1741 tttcgaggtg gatacggtgt cgttgcacgt ccacgagcgc atcgacccgg ccagcatcct
     1801 ctccgcgatc cgaaaaaacg tagggcggat tagtccgtca ggacgtaacc cgccgaatgc
     1861 gtacatgcac caacccgacc tcttcgccgc gccattcgaa aacctgccgc tgcgcgacgc
     1921 catcgacttc tacaagcacg aacgcggctg ggccaaccgg ctgatcgcgg gcgattcgct
     1981 gctggtgatg aactcgctgt tacagaagga aagcctggcc gggcaggtgc agatgatcta
     2041 catcgatccg ccctacggca tcaagtatgg ctccaacttc cagcccttcg tcaacaagcg
     2101 cgacgtgaag gaccgcaagg acgaagacct gacccaggaa ccggagatga tcaaggcttt
     2161 ccgcgatacc tgggagctgg gcatccattc ctatcttacg tatttgcgcg accgactgct
     2221 gctggcaaag gatttactgc acgagtcggg gagcgtgttt gtgcagattt cggatgagaa
     2281 tctgcatcac gcaactgaat tgtgcgatga gatttttggg cctcagaacc gaatctcaat
     2341 catcgccttc aagaaaacga accgtgctct cgggagcaag tatgtgggtg gtgttaccga
     2401 ctacatcatt tggtacgcaa aagatgagtt gaaattgaag ttccggactc tgttcgagct
     2461 gaaagaagcg ggagaggttg gtggaacgaa ttatcagttc gctgagctga cgagtggtca
     2521 gcgggttaga acaagtgcca ttgacgaggg cgagataaaa gatgttcgca ttttctcgcc
     2581 cgtagatctg atggcttcgt tctatacggc ttcatgtgac tatgaaataa attttcaagg
     2641 ccggtctttt acgccgcggc ctggaaatag ttgggcaacg catcccgcag ggacacagcg
     2701 tcttgcgaag tccgcgcgta tgatgatggt aggcgagcgc ccctattatg ttgcgtatct
     2761 attggactat ccggtactgc ctctgtccaa tctctggact gacacacgag gggagatgtc
     2821 caagatatac accgttcaaa ctacgcaaag ggtaatcgaa cgctgcctcc tcatgaccac
     2881 cgaccccggc gacctggtgc tggaccccac ctgcggttcc ggcaccacgg cctacgtcgc
     2941 cgagaagtgg ggccggcgct ggattaccac cgatacctcg cgcgtggcgg tgacgctcgc
     3001 caagcagcgg ctcatgaccg cgagctacga ctactatgcg ctgcgctatc cgcaggaagg
     3061 cctgaagggc ggcttcatct acaagaccgt gccgcatgtc acgctcaagt ccatcgccaa
     3121 caatccggag atcgacgaaa tccacgcgcg catgtatccg gcggttgagg tggcgctcga
     3181 tcagcttaac gaagcattgg gtagggtgcg tcccacgcac caaaaacgcg gtgcgcaggg
     3241 cgcaccctac atgaatgagt gggaggtacc gttcgatttc cccgaagact ggccgcaatc
     3301 cgcgcgcaaa ccgttcgagg ccttccacgc tgcgcggcag gcgatgcaaa agcaaatgga
     3361 cgcttccatc gccgcgcacg ccgatcagga aacgctctac gaccagccac agcccgacaa
     3421 gaacaagctg cgcatcaccg gcccctttac cgtcgaggcc gtgccgttcc cgaccgtgct
     3481 cgcgctcgat gaggccaagc ccgcgcaaga ggcggacgtg gcgattgccc gctccggcga
     3541 atcctggcgt cagcatcagt ggcgcgatga gttattgaaa gccggcatcc gcggcaaggg
     3601 cgggcagatg ctcaagttcg cggagttgga gacattcccg gcatcggagc atattcgcca
     3661 cctgcactgt agcggcacct tggccgagac cggagagcgc gtagtggtga gcttcggccc
     3721 cgagcacgcg gcgctggaac agcggcaggt aaagctggcg ctggatgagg ccgaaaaact
     3781 tcggccagcg ccgaagttca tcatcttcgc atctttcgag ttcgatcccg aggcgggcca
     3841 aggacattga cgaaatcaaa tggccgggca tgaccctgct caaggccaag atgaacaccg
     3901 atctcctgac tg
//
LOCUS       DBNGZE010000284         5958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1289914, whole genome shotgun sequence.
ACCESSION   DBNGZE010000284 DBNGZE010000000
VERSION     DBNGZE010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1289914"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC16FV_07330"
     CDS             <1..423
                     /locus_tag="AC16FV_07330"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR2849904.1"
                     /translation="AARLAAADVGTLAIAGDTAAQLYGLHMFANNIEDRPDNTTRFLV
                     IGTLETSPSGNDKTSLLLSSRNRPGALQRLLAPLAKNKINMTRIESRPSRQSMWEYVF
                     FVDIDGHMKDRKVAKALADLEKEAAFLKRLGSYPKAVL"
     gene            420..1532
                     /gene="hisC"
                     /locus_tag="AC16FV_07335"
     CDS             420..1532
                     /gene="hisC"
                     /locus_tag="AC16FV_07335"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR2849905.1"
                     /translation="MTATVADILRLATPGVQGLSPYQPGKPIEELEREYGVTGAIKLA
                     SNENALGPSPRALAAAREALADIHRYPDGNGFVLKSALAKKHGVKPECLTLGNGSNDI
                     LEFLARAFVLPENEVIFSEHAFAVYPIVTRAVGAKAVITRAKYWGHDLAAMRAAVNAR
                     TRLVFIANPNNPTGTWLKVDELESFVRDLPAHVLVAVDEAYFDYVEEREYPNTIAWCA
                     KYPNLVTARTFSKAYGLAGLRVGYGISSPAVADVLNRVRQPFNVNSVALAAASAALDD
                     TAHVARAVQTNRDGMRQLIEAFKELGLEYIPSVGNFICVDFKKPASGLYEAFLRQGVI
                     VRPVANYGMPNHLRITVGMPEENRRFIEALRKIVRD"
     gene            1525..2388
                     /locus_tag="AC16FV_07340"
     CDS             1525..2388
                     /locus_tag="AC16FV_07340"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR2849906.1"
                     /translation="MIERLCIIGIGLIGGSLARALRDAGYVREIIGYGRSVGNLQQAV
                     ELGVIDRAEVSLPDAVLGADMIVLAVPVGNMADILNTLGPVLPEQAVVTDVGSVKGSV
                     IRSARAALGARFAHFVPGHPIAGTEQSGVAASLPDLFQRRRVILTPEAETDGDALARV
                     RAMWEAAGAQVAMLTAVDHDRILAVSSHLPHMLAYCLMDMVVRHDDHRAILENSAGGF
                     SDTTRIAASDPVMWRDICLANRDALLDAMRQYHTDLGMFIEAIEKGDGKWLCETFTRA
                     KHARDGLNKKY"
     gene            2493..3803
                     /gene="aroA"
                     /locus_tag="AC16FV_07345"
     CDS             2493..3803
                     /gene="aroA"
                     /locus_tag="AC16FV_07345"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR2849907.1"
                     /translation="MNKSIDYLVQPGGKLTGRVRVPGDKSISHRSVMLGSLAEGETRV
                     TGLLEGEDVLSTLGAFCAMGVHAEGPSNGKLVIQGAGLHGLKAPTKPLDMGNSGTAMR
                     LMAGILAGQPFDSELIGDESLSKRPMKRVSEPLGRMGAVIETTEGGRPPLLIKGGQKL
                     HGIDYQMPMASAQVKSSLLLAGLYAEGETTVTEPAPTRDHTERMLRGFGYNVKTKGAR
                     ISLHGGGRLKGCNIDVPADISSATFFLVGASIAPGSDLVLEHVGINPTRIGVINILKL
                     MGANLELLNMREVGGEPVADIHVRAAKLRGIRIPEDQVPLAIDEFPALFIAAAAASGE
                     TVLTGAHELRVKESDRIAVMAQGLKQLGIDARETPDGIIIKGGRLRGGEIDSHGDHRI
                     AMSFAMAGLVAAGPITVRDCKNVDTSFPGFVSLAAQTGLSIRAI"
     gene            3933..4622
                     /gene="cmk"
                     /locus_tag="AC16FV_07350"
     CDS             3933..4622
                     /gene="cmk"
                     /locus_tag="AC16FV_07350"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR2849908.1"
                     /translation="MNSSIPVLTIDGPSGSGKGTVGQILAQRLGWHFLDSGALYRALG
                     VAAKQAGVSFEDRPALAQLASSMDIRFIPRADGSPAAVLLHGAEIGDQLRTEESGKLA
                     SIVAAIPEVRRALLQKQHSFRRSPGLVADGRDMGSTVFPDAILKVYLTASPEVRAERR
                     YKQLIEKGFDVNLPRLLDEIRERDARDAGRAVSPLKPAEDACILDTSQLDISGVVERV
                     HGLLQQRQRPG"
     gene            4737..>5958
                     /gene="rpsA"
                     /locus_tag="AC16FV_07355"
     CDS             4737..>5958
                     /gene="rpsA"
                     /locus_tag="AC16FV_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR2849909.1"
                     /translation="MTESFAQLFEESLNKIHLQSGELITGEVVHVDENVVVVNAGLKS
                     EAVIPVAEFQDGRGEVTVKVGDTVEVVIEMLEDGSGETRLSREKACRAKAWEDLEKAF
                     EDQSIVTGVMTGKVKGGYTVAMNTIRAFLPSSLVDVRPVRDPSYLEGKELEFKVIKID
                     RKRNNVVVSRRAVVEKEISAEREALLSGLEEGQTVKGVVKNLTDYGAFIDLGGIDGLL
                     HITDLAWKRVKHPSEVLKIGDEIEAKVLRYDRERNRVSLGLKQLGDDPWVDIQRRYPE
                     GTRLFGKVTNITDYGAFVEIEPGVEGLVHVSEMDWTNKNIHPTKVVQLGDEVEVMILD
                     IDTERRRISLGMKQCQPNPWEEFAANHNKNDKVRGQIKSITDFGVFVGLPGGIDGLVH
                     LSDLSWSLPGEAAVR"
BASE COUNT         1198 a         1750 c         1882 g         1128 t
ORIGIN      
        1 gcggcgcggc tcgcggcggc ggacgtcggc acgctcgcga tcgccggtga caccgcggcg
       61 cagctctatg gtctgcacat gttcgccaat aacatcgaag accgtccgga caacaccacg
      121 cgcttcctcg tgataggcac gctcgaaact tcaccgagcg gcaacgacaa gaccagtctg
      181 ctgctctcca gccgcaaccg tcccggtgcg ttgcagcgcc tgctcgcgcc actggcgaag
      241 aacaagatca acatgacacg catcgagtcg cgcccctcgc gccagagcat gtgggagtat
      301 gtcttcttcg tcgacattga cggccacatg aaggatcgca aggtcgccaa ggcgctcgcc
      361 gatctggaaa aggaagcggc gttcctcaaa cggctcggtt cctatcccaa ggccgtgctg
      421 tgacagccac ggtggcggat atcctgcgcc tggcaactcc cggtgtgcag gggctctcgc
      481 cctatcagcc gggcaagccg atcgaggaat tagagcgcga atacggcgtc accggtgcca
      541 tcaaactcgc gagcaatgaa aacgccctcg gccccagccc gcgcgcactg gccgcggcgc
      601 gcgaggcgct ggcggacatc caccgctatc ccgacggcaa cggtttcgtg ctgaaaagcg
      661 cgctggcgaa aaaacacggc gtcaagccgg aatgtctcac gctcggcaac ggctccaacg
      721 atattctgga atttctggcg cgcgccttcg tgctgccgga aaacgaagtc atattctcgg
      781 aacacgcctt tgcagtctat cccatcgtga cgcgcgcggt cggcgcgaaa gcggtgatca
      841 ccagggcgaa gtactggggc cacgatcttg cggctatgcg cgcggcggtg aatgcacgca
      901 cgcgcctcgt tttcatcgcc aatcccaata atcccactgg cacttggctc aaggtcgatg
      961 aactcgaatc cttcgtccgc gacctgccag cgcatgtgtt ggtggcggtg gatgaggcct
     1021 actttgatta cgtcgaggaa cgggaatatc cgaataccat cgcctggtgc gcgaaatacc
     1081 cgaatctcgt taccgcgcgc accttttcca aggcctacgg actggccgga ttgcgcgtgg
     1141 gttatggtat ttccagcccg gccgtagccg atgtcctgaa ccgcgtgcgc cagccgttca
     1201 acgtgaacag cgtggcgctg gcagccgcca gcgcggcatt ggatgacacg gcgcatgtgg
     1261 cgcgggcggt gcagaccaac cgcgacggca tgcgtcagtt gatcgaggca ttcaaggaat
     1321 tggggctgga atatattccc tcggtgggaa atttcatctg cgtcgatttc aagaaaccgg
     1381 cgagcgggct gtacgaggcc tttttgcgtc agggcgtgat cgtgcggccg gtggctaatt
     1441 acgggatgcc gaatcatttg cgcatcactg tcggtatgcc ggaggagaac cggcgcttta
     1501 ttgaagcgct aaggaaaatt gtccgtgatt gagcggctct gcattatcgg catcggcctg
     1561 attggcggct cgctggcgcg tgccttgcgc gacgccggct atgtgcgtga aatcatcggc
     1621 tatggccgca gcgtcggcaa tttgcagcaa gccgtggaac tcggtgtgat agatcgtgcg
     1681 gaagtatcgt tgccggacgc cgtgctcggc gccgacatga tcgtgctcgc cgtgccggtg
     1741 ggcaatatgg ctgatatcct gaacacgctc ggtccggtgc tgccggagca ggcggtggtg
     1801 accgatgtcg gcagcgtcaa aggcagcgtc ataagatccg cgcgcgcggc gctgggtgca
     1861 cgcttcgccc atttcgttcc cggacatccc attgccggca ccgagcaatc gggcgtggcg
     1921 gcgtcgctgc cggatttgtt tcagcgccgg cgcgtgattt tgacccccga agccgaaacg
     1981 gatggcgatg cgctggcgcg cgtgcgcgcc atgtgggagg cggcgggcgc acaggtggcg
     2041 atgctcacag ccgtggatca cgatcgcatt ctcgccgtta gcagtcatct gccgcacatg
     2101 ttggcctact gcctgatgga catggtggtg cggcacgacg atcaccgcgc catcctggaa
     2161 aattccgccg gcggcttcag cgacaccacg cgcatcgcgg ccagcgatcc ggtaatgtgg
     2221 cgcgacatct gcctcgccaa ccgcgatgcg ctgctggatg cgatgcgtca gtatcatacc
     2281 gacctcggca tgttcatcga ggcgatagag aagggcgacg gcaagtggct gtgtgagacc
     2341 ttcacacgcg ccaagcacgc gagagatggt ttgaataaaa aatattaata ttttgtaacc
     2401 accgatgaac acggatgaac accgataatt gcaaaaactg ttgtttgttt tatccgtgtt
     2461 aatctgtgtt catccgtggt tccagattca ttatgaataa atccatcgat tatttggtgc
     2521 agcctggtgg aaaattgacc ggccgggtgc gcgtgccggg tgacaagtcc atttcccatc
     2581 gctcggtcat gctgggctcg ctcgcggagg gcgagacgcg cgtaaccggc ctgctggaag
     2641 gcgaagacgt gctgtccacg ctgggcgctt tttgcgccat gggcgtgcac gccgagggcc
     2701 cgagcaatgg aaagctggtt attcagggcg ccggtctgca tggcctgaaa gcgccgacca
     2761 agccgctcga catgggcaat tccggtacgg ccatgcgcct tatggccggc atcctcgcag
     2821 gccagccgtt cgattccgaa ttgatcggcg atgaatcgct gagcaagcgc ccgatgaagc
     2881 gtgtttcgga accgctcggt cgcatgggcg ccgtcatcga aacgacagaa ggaggtcgcc
     2941 cgccgttact gatcaagggc gggcaaaagt tgcatggcat tgactatcag atgccgatgg
     3001 cgagcgcgca ggtgaaatcc tcgctgctgt tggccgggct gtatgccgaa ggcgagacca
     3061 cggtcactga gccggcgccg acgcgcgatc acaccgaacg catgctgcgt ggtttcggat
     3121 acaacgtgaa aacgaaaggc gcacgcatca gcctgcatgg cggcggcagg ctcaagggtt
     3181 gcaatatcga tgtcccggcg gatatttcct cggcgacatt ttttctcgtc ggcgccagca
     3241 tcgcacccgg ctccgatctg gtgctggagc atgtcggcat caacccgacg cgcatcggcg
     3301 tgatcaatat tctgaaactc atgggtgcga acctcgaact gctgaatatg cgcgaagtcg
     3361 ggggcgagcc ggtggcggat atccacgtgc gcgcggcaaa gctgcgcggc attcgcatcc
     3421 cggaagacca ggtgccgctc gccatcgacg aattcccggc gctgttcatc gccgctgccg
     3481 cggcttccgg cgaaaccgtt ctgaccggcg cgcatgaatt gcgcgtgaag gaatcggacc
     3541 gtattgcggt aatggcgcaa ggcctcaaac agctcggcat cgatgcccgc gaaaccccgg
     3601 atggcatcat tattaaaggt ggacgcctgc ggggcggtga gatagacagc cacggcgatc
     3661 atcgcatcgc gatgtcattc gcgatggcgg ggcttgttgc cgccggcccg atcacggtgc
     3721 gcgactgcaa gaacgtggat acctcgttcc cgggtttcgt cagcctcgcg gcgcagacag
     3781 gcctgtctat tcgagctatc tagataatat tttggaacgc agataaacgc agatctaaat
     3841 aagatgaacg cggattggct ggatattttc tttcgctgtt atctgcgttt atcaaattat
     3901 gaatccgcgt ccatctgcgg ttacacgatc atatgaattc cagcattcca gtgttgacca
     3961 tcgacggccc gagcggatcg gggaagggca cggtcgggca gattctggcg caacggctcg
     4021 gctggcattt tctcgacagc ggggccctgt accgggcgct cggggtggca gcgaaacagg
     4081 ccggggtcag cttcgaggat cgaccggcgc tggcgcaact ggccagttcc atggatatcc
     4141 gttttatccc ccgcgccgac ggcagcccgg cggcggtgct gttgcatggt gctgagatcg
     4201 gcgaccagct gcggacggaa gaaagcggca agctggcctc gatcgtggcc gcaatacccg
     4261 aggtgcgccg ggccttgttg cagaaacaac actcgttccg ccggtcaccg ggcctggtgg
     4321 cggacggccg ggacatgggg tccacggtct tccccgacgc gatacttaag gtgtatctca
     4381 ccgccagccc cgaggtgcgc gccgagaggc ggtataaaca gttgatagaa aagggattcg
     4441 atgttaacct tccgcggctt ttggacgaga tccgtgagcg cgatgcgcgc gacgccgggc
     4501 gggcggtttc accgctcaag ccggcggaag atgcgtgcat cctggatacc tctcagctcg
     4561 acatttccgg ggtggttgag cgtgtgcatg gcctgctgca gcaacggcaa cggccggggt
     4621 agtcgtgcct ttccagtgaa cggacggggc ctcaggatct ccctttaaac caacccgagt
     4681 aacgaagtcg ctgagtcacg atacgcggcc ggtgacagga accaaccttg acctttatga
     4741 ctgaaagttt cgcgcaatta tttgaagaga gcctgaataa aatccacctg caatccggcg
     4801 agctgatcac cggcgaagtc gtgcatgtgg atgaaaacgt agtggtggtc aacgccggac
     4861 tcaagtccga agctgtgatc cccgtggccg agttccagga cggccgcggc gaagtgaccg
     4921 tcaaggtcgg cgacaccgtg gaagtcgtga ttgaaatgct cgaagacggg tccggcgaga
     4981 cccgcctgtc gcgcgagaag gcctgccgcg ccaaggcatg ggaagatctc gagaaggcat
     5041 tcgaagatca atctatcgtc accggcgtca tgaccggcaa ggtgaagggc ggttacaccg
     5101 tcgccatgaa caccatccgc gccttcctcc ccagctccct ggtcgatgtc cgccccgtgc
     5161 gcgacccgag ctatctcgaa ggcaaggaac tcgaattcaa ggtcatcaag atcgatcgca
     5221 agcgcaacaa cgtggtggtg tcgcgtcgtg ccgtggtcga gaaggaaatc tccgccgagc
     5281 gcgaggcgct gctctcgggt ctcgaagaag gccagaccgt caagggcgta gtcaagaacc
     5341 tcaccgatta cggcgcgttt atcgacctcg gcggcatcga cggcctgctg cacatcaccg
     5401 atctggcgtg gaaacgcgtc aagcatccga gcgaagtgct caagatcggc gacgagatcg
     5461 aagccaaggt gctgcgctac gaccgcgaac gcaaccgcgt ttcgctgggc ctcaagcagc
     5521 tcggcgacga tccgtgggtc gacatccagc gccgctatcc ggaaggcacg cgcctgttcg
     5581 gcaaagtcac caacatcacc gactatggcg cgttcgtgga aatcgagccg ggcgtggaag
     5641 gcctggtgca cgtgtccgag atggactgga ccaacaagaa catccatccg accaaggtcg
     5701 tgcagctggg cgacgaggtc gaggtgatga ttctcgacat cgacaccgag cgccgccgca
     5761 tttcgctggg catgaagcag tgccagccga atccgtggga agagttcgcc gccaaccaca
     5821 acaagaacga caaggtccgc ggccagatca agtcgatcac cgacttcggc gtgttcgtcg
     5881 gtcttcccgg cggcatcgat ggcctcgtgc acttgtcgga tctgtcttgg tcgctgcccg
     5941 gcgaagccgc ggtgcgcg
//
LOCUS       DBNGZE010000285         2729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1915316, whole genome shotgun sequence.
ACCESSION   DBNGZE010000285 DBNGZE010000000
VERSION     DBNGZE010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1915316"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC16FV_07360"
     CDS             complement(<1..385)
                     /locus_tag="AC16FV_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR2849910.1"
                     /translation="MNAKPDILGVKHIGLAHGNGGRLMRELIERIFARHLSNPLLDVQ
                     ADAVPLPTPDGELMFTTDGFTVQPLEFPGGDIGSLAVHGTVNDLAVAGATPLYFSLNA
                     FIEEGLEVAQLERIIESLGRAATAAG"
     gene            complement(382..2679)
                     /gene="hypF"
                     /locus_tag="AC16FV_07365"
     CDS             complement(382..2679)
                     /gene="hypF"
                     /locus_tag="AC16FV_07365"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR2849911.1"
                     /translation="MSKRDLPLTSPDSGTARQALRLTVSGRVQGVGFRPFVYRLAHRL
                     GLRGWVRNETGTVEIWVEGEASPVETFEQVLLEQAPPLARPKIATRSPAEPQAFETFE
                     ILPSRAEAPARIHVPPDYFACDDCLRELRDPENRRYRHPFINCTQCGPRYTLIGKLPY
                     DRPNTSMAAFAMCPECRREYENPLDRRFHAEPVACPACGPRLQFSVPGGAMVRDNSAA
                     LTQAVAALRAGKIVAVKGIGGYHLMCDARNDIAVARLRVKKPRPHKPLAVMVPARGTD
                     GLEAVRVVAELTPEHEQWLRDPVRPIVLVNKKPHAPLSPRIAPGLREVGMMLPYSPLH
                     HMLLDEFGGPLVATSGNVSGEPVLTENDDVNQRLAHVAEAFLHHDRPIQRPADDPVYR
                     VIGGFARPLRHGRGSAPLELTLPFTLHKPVLAVGGHMKNTVAFAWDDRIVVSPHIGDL
                     DAPRSLSVFEQVITDLQKLYGVRAEYIVCDAHPVYVSSRWARRSGLPITQVLHHYAHA
                     SALAGEHPDVKNWLVFSWDGVGYGADGTLWGGEALLGAPGRWQRAGSLRPFYLPGGEK
                     AGREPWRSALSLCWEAGIEWKQCPEDPMLAHHAWRRRLNCPQTTSAGRLFDAAAALTG
                     LHHRSSYEGQGPMLLEAACTEDSAQAIPLQLARNRENLWETDWAPLLPFLMNTKLTVT
                     ERADGFHESLARALVAQANALREEHGEVAVGLGGGVFQNRVLTERAFAALRENGFDVR
                     LMHQVPCNDAGISYGQIIEAGARLA"
BASE COUNT          449 a          890 c          909 g          481 t
ORIGIN      
        1 cgccggccgc cgtggcggcg cgcccgaggc tttcgatgat gcgttcgagt tgcgccactt
       61 ccaggccttc ttcaataaag gcattaagac tgaaatagag tggcgtcgcg cccgcgaccg
      121 caagatcgtt gaccgtgccg tgcaccgcga gcgagccgat gtcgccgccg ggaaattcca
      181 atggctgcac cgtgaagccg tcggtggtga acatgagttc gccgtccggc gtgggtagcg
      241 gcacggcgtc ggcctgcacg tccagcagcg gattggaaag atggcgcgcg aaaatccgct
      301 cgatcagctc gcgcatcagg cgtccgccat tgccgtgcgc cagcccgatg tgtttgacgc
      361 cgagaatgtc gggtttggcg ttcatgcgag cctggcaccg gcctcgatga tctggccgta
      421 gctgatgccg gcgtcgttgc acggcacctg gtgcatgaga cgcacgtcga agccgttttc
      481 gcgcagcgct gcgaaggcgc gctccgtcag cacgcggttc tggaacacgc cgccgcccag
      541 acccacggcc acctcgccat gttcctcgcg cagtgcattg gcctgcgcca ccagcgcgcg
      601 cgcgaggctt tcgtggaatc catcggcgcg ctcggtgacg gtgagttttg tgttcatcag
      661 gaagggcagc agtggcgccc agtcggtttc ccacaggttc tcgcgattgc gcgccagctg
      721 caatggtatc gcctgcgcgc tgtcctcggt gcaggcggct tcgagcagca tcgggccctg
      781 gccttcgtag cttgaacgat gatgcagccc cgtcagcgcc gccgcggcat cgaagagtct
      841 gccggccgat gtcgtttgcg ggcaattcaa acgccgccgc caggcgtgat gcgccagcat
      901 cgggtcctcg gggcattgct tccattcgat accggcctcc cagcacagtg acaacgccga
      961 gcgccacggt tcgcgcccgg ccttctcgcc gccggggaga tagaaaggcc gcaggctgcc
     1021 cgcgcgctgc cagcgtccgg gcgcgcccag taaggcctcg ccgccccaaa gcgtgccgtc
     1081 ggcgccgtaa cccacgccgt cccacgagaa caccagccag tttttgacgt ccgggtgctc
     1141 accggcgagg gccgaggcgt gcgcgtaatg gtgcagtacc tgggtgatcg gcaggccgga
     1201 gcgacgcgcc caacgcgagc tgacgtatac cgggtgcgcg tcgcacacga tgtactccgc
     1261 gcgcacgcca taaagttttt gcagatcggt aatcacctgc tcgaacacgc tgagactgcg
     1321 cggtgcgtcg agatcgccaa tgtgcggcga cacgacgatg cgatcgtccc acgcgaacgc
     1381 gacggtgttt ttcatatgtc cgccgacggc gagcaccggt ttgtggagcg tgaacggcag
     1441 cgtgagttcc agcggcgcgc tgccgcgccc gtgacgcaac ggccgcgcga aaccaccgat
     1501 cacgcgatac accggatcgt ccgccggacg ctggatcggg cggtcgtgat gcaggaacgc
     1561 ctcggcgaca tgcgccagcc gctgattgac gtcgtcattt tcggtcagca ccggttcacc
     1621 gctgacgttg cccgaggtcg ccaccagcgg cccgccgaac tcgtccagca gcatatgatg
     1681 cagcgggctg tacggcagca tcatgccgac ctcgcgcagc cctggcgcga tgcgcggtga
     1741 cagcggagcg tgcggttttt tatttaccag cacgatggga cgcacgggat cgcgcaacca
     1801 ttgttcgtgt tccggcgtca gctccgccac gacgcgtacc gcctccagcc catccgtgcc
     1861 gcgcgcggga accattacgg ccagcggttt gtgtgggcgc ggttttttaa cgcgcaggcg
     1921 cgcaacggca atgtcgttgc gggcgtcgca catcaggtga tagccgccga tgcccttgac
     1981 cgcgacgatc ttgccggcgc gcaacgcggc gacggcctgc gtcagcgcgg cgctgttgtc
     2041 gcggaccatg gcaccgcctg gcacactgaa ctgcaagcgt ggtccgcaag ccgggcaggc
     2101 gaccggttcg gcatgaaacc gccgatcgag cgggttttcg tattcgcgcc ggcattccgg
     2161 gcacatcgca aacgccgcca tgctcgtgtt gggccggtcg tagggaagct tcccgatgag
     2221 cgtgtagcgc ggtccgcact gggtgcagtt gatgaacgga tggcggtaac ggcgattctc
     2281 cgggtcgcgc aactcgcgca ggcaatcatc gcaggcgaaa taatccggcg gcacatggat
     2341 gcgcgccggg gcctcggcgc ggctgggaag gatttcgaag gtctcgaagg cttgcggttc
     2401 ggcgggcgag cgcgtggcta ttttcggacg cgccagcggc ggtgcctgtt ccagcaagac
     2461 ctgctcgaaa gtctcgactg gcgatgcctc accttccacc catatctcga ccgtgccggt
     2521 ttcgttgcgc acccagccgc gcaggccgag ccggtgcgcc aggcgataga caaacgggcg
     2581 gaagcccacg ccctgcacgc gtccgctgac tgtcagacgc agcgcctgtc ttgctgttcc
     2641 tgaatcaggg ctggtaagag gaaggtctcg cttgctcatg tgggtattgt tcaatgcgcg
     2701 tgcgcagggg gcatcttccc gccataggc
//
LOCUS       DBNGZE010000286        10345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_804771, whole genome shotgun sequence.
ACCESSION   DBNGZE010000286 DBNGZE010000000
VERSION     DBNGZE010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_804771"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16FV_07370"
     CDS             complement(<1..425)
                     /locus_tag="AC16FV_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR2849912.1"
                     /translation="MSISLPVPAGVALRAEVPTEYASILTPEALDFVAGLARAFGPER
                     EQLLARRVERQAQLDRGIKPEFPPETRSVREADWTIAPVPAEIQDRRVEITGPVERKM
                     IINALNSGADVYMADFEDANTPNWFNQIQGQINIKDAVRR"
     gene            456..707
                     /locus_tag="AC16FV_07375"
     CDS             456..707
                     /locus_tag="AC16FV_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849913.1"
                     /translation="MIDISARHGRFASLIGYQAVWSGMSGFGNNRPRETLIWLRLLNI
                     SGFFGRAPSFPAVSRVESRPFSNLVRALSGRAGLFTEVR"
     gene            713..961
                     /gene="rpsP"
                     /locus_tag="AC16FV_07380"
     CDS             713..961
                     /gene="rpsP"
                     /locus_tag="AC16FV_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR2849914.1"
                     /translation="MVTLRLARGGANKRPYYRIIVADRRRALNGNNIERIGFYNPLAT
                     EKETKLQVNMERVQYWLSKGAKPTDRVADLLKRAAKSS"
     gene            968..1483
                     /gene="rimM"
                     /locus_tag="AC16FV_07385"
     CDS             968..1483
                     /gene="rimM"
                     /locus_tag="AC16FV_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930638.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR2849915.1"
                     /translation="MTNPSRPDGLVHFGRITGLFGVQGWVKVFSHARPREAIIDYSPW
                     FVKTAEGWREMVVEDGRAQGKGVVAKLVGVDDRDQASKLIGADIEIKFSQLPPPSKGE
                     YYWAQLVGLEVVNLAGQSFGKVDHLVETGANDVLVVRNGKERWLPVTPNVIREVNLEA
                     GVIRVDWDADF"
     gene            1487..2245
                     /gene="trmD"
                     /locus_tag="AC16FV_07390"
     CDS             1487..2245
                     /gene="trmD"
                     /locus_tag="AC16FV_07390"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR2849916.1"
                     /translation="MRIDVISLFPPMFEAVSAHGITGRAVKNGLLTLRVWNPRDYTED
                     RHRSVDDRPYGGGPGMVMMIEPLERAIRAARGEQRKEAKVIYLSPQGRRLDHAAVLEL
                     SKREGMILLAGRYEGIDERLLETEVDEEWSIGDYVLSGGELAAMVLIDAVTRQLPGAL
                     GDEDSAQQDSFVEGLLDCEHYTRPEEYTGRKVPEVLLSGDHEKIRRWRLKRSLGRTWL
                     RRPDLLEKLTLNGEQKQLLEEFQREYLKESGVEK"
     gene            2242..2589
                     /gene="rplS"
                     /locus_tag="AC16FV_07395"
     CDS             2242..2589
                     /gene="rplS"
                     /locus_tag="AC16FV_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR2849917.1"
                     /translation="MSKIMQELETRMMADKKLPEFAPGDTVTVHVKVQEGERERLQAF
                     EGMVIARSNNGYNSSFTVRKNSYGEGVERVFPLYSSAIAKIEVNRKGDVRRAKLYYLR
                     DLAGKAARIREKV"
     gene            3045..3566
                     /locus_tag="AC16FV_07400"
     CDS             3045..3566
                     /locus_tag="AC16FV_07400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR2849918.1"
                     /translation="MALIVVHMDNETMRRGKRARDFDAVIAAPFGRVGFILDGDAVTD
                     ISFLDKNTPLLPPRSAQARKVSRVLRSYFTNPKVSFRLPLKTSGTAFQQRVWRALRRI
                     PAGRTLSYGVLAQKLDTSARAVGNACRTNPIPIIIPCHRVVASNGVGGFMGKRSGSPL
                     NLKHWLLKHERRK"
     gene            3550..4449
                     /gene="xerD"
                     /locus_tag="AC16FV_07405"
     CDS             3550..4449
                     /gene="xerD"
                     /locus_tag="AC16FV_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR2849919.1"
                     /translation="MSAGNKDLIERFCDALWLQEGLSANTLEAYRRDLEGLDRWITKR
                     GTALMSASRADLLEYLSSNVGKGARPRTTARLLSSLRRFYGYLVREEKIKTDPTALID
                     SPKLGRPLPKSLSEEQVEKLLESPDIKTSLGLRDRAMLETLYATGLRVSELVGLTLSQ
                     VSLTQGVVRVIGKGDKERLVPLGEEAIAWISRYLAEGRPKLVKSRSTDALFTTARGGP
                     MTRQAFWHNLKTHTRKAGIDAKKLSPHTLRHAFATHLLNHGADLRVVQMLLGHADLST
                     TQIYTHVAQARLKTLHEQHHPRG"
     gene            complement(4452..5210)
                     /locus_tag="AC16FV_07410"
     CDS             complement(4452..5210)
                     /locus_tag="AC16FV_07410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR2849920.1"
                     /translation="MIRSTFTNKYGEDWEFEYNSELEKAIVKGSDVSWEEYPVIEGIA
                     CGLYMSKEEHEWLLSAWKAATQDKPNTGLYLGRDTEFQAGQDSCWLTNDYCPICLCKR
                     REFEVHHCIWSCDGGTYDASNLLHICNSCHAVITRGSIEDRFPKNKAALNHQLMYFGL
                     EFFPRNNPTKGRHTHLSFLEKNPFFKEELLGCDRLAETEKEHIDREIKRMARIEYQYF
                     RDMSLGKWSWEQYEIRELKPSLEFYRSLKEDNPT"
     gene            complement(5758..6162)
                     /locus_tag="AC16FV_07415"
     CDS             complement(5758..6162)
                     /locus_tag="AC16FV_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849921.1"
                     /translation="MKGPTTATAPSKEKKKTSPAAWVALLLIIGGIVWYFQSREFQEQ
                     SLPPMPVEVAFRGALLGPGLVLQVKNKSTRHLSILVTLKNPSMQQGKNYRLDVAPQGM
                     SEIGHMEGWTLASGDNIELFHNDYQSWKGKIP"
     gene            complement(6166..6240)
                     /locus_tag="AC16FV_07420"
                     /pseudo
     CDS             complement(6166..6240)
                     /locus_tag="AC16FV_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024645.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(6691..7425)
                     /locus_tag="AC16FV_07425"
     CDS             complement(6691..7425)
                     /locus_tag="AC16FV_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849922.1"
                     /translation="MSKRRKERARQRELAQDFSGESLTPEGFHTFYTKFINLRFPMKI
                     AHVLELRYLINHAVDHYREPPVTPSYRQFRESLQSALDSFAIENRRYSERMLKILSMF
                     RDIHYAHSIASRDAERQLRESMERNRNDYARAVRRGLFFVFAGVSFIVFWLATPSAHL
                     VIKLLPALCAWFALRNFHKLPALEKQHEKLTLEVNDVLRRRVNSLNWKTLIHKLALVL
                     GYKRVPGVEVFDVDIDHEQINRSAYH"
     gene            complement(7711..9723)
                     /locus_tag="AC16FV_07430"
     CDS             complement(7711..9723)
                     /locus_tag="AC16FV_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR2849923.1"
                     /translation="MPKLNPQQKAAVQYINGPLLVLAGAGSGKTSVITRKISWLIRDY
                     GIAPRNIVAVTFTNKAAREMKSRVADLMNGDYSKELLISTFHTFGLKILREHLDQIGY
                     RPGFSLYDGEDSQALLSKLLRGQYSGKNNPAEAVRHQISMWKNNLISPAEAPLAAGGE
                     SIQDVAARVYGEYERHLQAYNAMDLDDLILKPVRLFQNQPEILEEWRRRVRYLLVDEY
                     QDTNLCQYELVKLLAGHRGALTVVGDDDQSIYTWRGAHPENLKQLQQDFPMLKVIKLE
                     QNYRSSGRILKAANALIANNPHIFEKALWCDRDYGVPLRVLRARGDEHEAERVVSELL
                     YHKFKHNTDFRDYAILFRENNQSRVLERVLRERRIPYFLSGGTSFFDKTEIKDVMAYL
                     RLMCNPGDDNAFLRVINTPRREIGPATLEQLAAHAAELNTSLLQASMDPGIDTKLTAR
                     QETTLRSFTHWLMEMITKSEDEEPGKLVCDMLANLHYEEWLKDTCNDHKIAQWRMENV
                     LELVSWIQRLARQEEDLTLSDLVARLTLSGILDKGDQENPGDLVSLMTVHAAKGLEFP
                     HVFIVGMEEGLLPHHQSQSDAGVEEERRLAYVAMTRARQTLTFSYADRRKRGGEVLVC
                     EPSRFLKEMPADDLKWEEPDREPDPQAMLGRADVYLANLRNMLGNS"
     gene            complement(9820..>10345)
                     /locus_tag="AC16FV_07435"
     CDS             complement(9820..>10345)
                     /locus_tag="AC16FV_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR2849924.1"
                     /translation="PAFAQAGAARAESFDRLRDLFAGTSLYLEHTTDIEGISWSAVLK
                     NVYAILFGVADELGLGDNMRGYLATAAMHELERIVAGLGGSAGAAHRLAGLGDLITTA
                     TSQGSRHHELGCRLVRGETAALEGEGVHTLKMVRTHRLFDPRPHPLFDLAGRLLDKPS
                     AARELMKNFLEQLA"
BASE COUNT         2067 a         2903 c         3109 g         2266 t
ORIGIN      
        1 cggcgcacgg cgtccttgat gttgatctga ccttggatct ggttgaacca gttcggggtg
       61 ttggcgtcct cgaagtcggc catgtacacg tccgcccccg agttgagcgc gttgatgatc
      121 atcttgcgct ccaccgggcc ggtgatctcg acgcggcggt cctggatctc cgccggcacc
      181 ggcgcgatgg tccaatcggc ctcgcgcacc gaccgggtct cgggcgggaa ttccggtttg
      241 atgccccggt cgagctgcgc ctgccgctcc acgcgccgcg ccagcagctg ctcccgctca
      301 ggcccgaagg cgcgcgcgag accggcgacg aaatcgagtg cttccggggt caggatgctg
      361 gcatattcgg tgggaacctc ggcccggagg gccacgccgg cggggacggg taaagatatg
      421 ctcatattgc cccctcggtc aggatcgagt tggtcatgat tgatatatct gcccgccacg
      481 gccgctttgc aagcctgatc ggatatcagg ccgtctggtc cggcatgagc ggatttggca
      541 ataaccggcc acgggaaacc ttgatctggc tcaggctgtt gaatatcagc gggtttttcg
      601 gccgtgcccc ttcatttccc gcggtatcga gggtagaatc gcgccctttc tcgaaccttg
      661 tacgggccct gtccggccgg gcggggcttt ttaccgaagt gaggtagatc gaatggtaac
      721 cttacgtctg gcccgtggcg gcgcgaacaa gcggccctat taccgcatca tcgtcgctga
      781 ccggcgtcgc gcgttgaacg gcaacaacat cgagcgcatc ggattttaca atccgctagc
      841 gaccgagaag gaaaccaagc tgcaggtgaa catggagcgc gtgcagtact ggctgagcaa
      901 aggcgccaag ccgacggatc gcgtcgctga tctgctgaag cgggccgcga aatcatcctg
      961 acgccttatg accaacccgt cacgccctga cggcttggtg catttcggcc ggatcaccgg
     1021 cctgtttggg gtacagggtt gggtcaaggt tttttcccac gcccgtccgc gcgaagcgat
     1081 catcgattac tcgccctggt ttgtgaaaac cgccgaaggc tggcgcgaga tggtcgtcga
     1141 agacggacgc gcgcagggca agggtgtggt ggcgaagctc gtgggcgtgg atgaccgcga
     1201 ccaggccagc aagctcatcg gcgcggacat cgagataaag ttttcgcagc tgccgccgcc
     1261 gtcgaaaggc gagtattact gggcgcagct ggtcgggctg gaggtggtca atctcgccgg
     1321 acaaagcttc ggcaaggtgg atcacctcgt cgagaccggc gcgaatgacg tgctggtggt
     1381 gcgcaacgga aaagagcgct ggctgccggt gacgccgaat gtcatccgcg aagtgaatct
     1441 ggaagcgggc gtgatccggg tggattggga cgcggatttc tgacccatgc gcatcgacgt
     1501 gataagtttg tttccgccga tgttcgaggc cgtgagcgct cacggcatca cggggcgggc
     1561 ggtcaagaac ggactgctga cgctgcgggt gtggaacccg cgcgattaca cggaagacag
     1621 gcaccgcagc gtggacgacc ggccttatgg cggcggcccg ggcatggtga tgatgattga
     1681 gccgctggag cgcgcgattc gcgcggcgcg cggcgaacag agaaaagagg cgaaggtgat
     1741 ttacctttcg ccgcagggcc ggcggctgga tcatgccgcg gtgctggagt tgtcgaagcg
     1801 ggaagggatg attcttctcg ccgggcgtta cgaaggcatt gatgagcgcc tgctcgagac
     1861 ggaagtggat gaggagtggt cgatcggcga ttacgttctc tccggcggtg aactggcggc
     1921 gatggtgttg atcgacgccg tgacgcgcca gcttcccgga gccctgggcg acgaggattc
     1981 ggcacagcag gattccttcg tggaaggcct gctggattgc gagcattaca cgcgcccgga
     2041 agagtacacc gggcgcaagg tgccggaagt gttgttgtcg ggcgatcacg aaaagatccg
     2101 tcgctggcgg ctgaagcgct cgctgggacg gacctggttg cggcggccgg atttgctgga
     2161 aaagctgacg ttgaatggtg aacagaaaca gctgctggag gaattccagc gggaatatct
     2221 taaagagtca ggagtcgaaa aatgagcaag atcatgcagg aactggaaac gcggatgatg
     2281 gcggacaaaa agctccccga gtttgccccc ggtgataccg ttaccgtgca cgtgaaggtg
     2341 caggaaggcg agcgcgaacg tctccaggcc ttcgagggca tggtcatcgc gcgcagcaac
     2401 aacggctaca actcctcttt cacggtgcgc aagaactcct acggcgaagg cgtggaacgc
     2461 gtgttcccgc tgtacagctc ggccatcgcc aagatcgagg tcaaccgcaa gggcgacgtt
     2521 cgccgcgcca agctctacta tctgcgcgat ctcgccggca aggccgcgcg tattcgcgag
     2581 aaggtctgag ttttgttgtc atgccggctg aagccggcat cccggtagta tcaaatagcc
     2641 gccctttggg cggcttttta tttgtatctt gtcgtcaccc cggcgaaggc cggggtccag
     2701 gcactaaatg gttcgcgcgc cctgcgcgcg actcacaagc tatgtgcggg tggctccgcc
     2761 ccgcaccctg ggtgactttt gtttcggcaa aagtcaccaa aaccactccc cccggagcgc
     2821 gcgacttcct tctccactcc tcgccccacc gggtgctcga ccaactcgcc gggcgcatgc
     2881 acccggggcg tgccgatccg cggcgcgccc gttcggcggc atggaagtcc accggacttc
     2941 catgaatact ccgcctcacc ctcgggctcg aacacggtct cgcttaaaaa ctcccggtgg
     3001 ggctgcggtg ctcgacgcgc gctacggggt ctgaaaaaac cgttgtggca ctaatcgttg
     3061 tgcacatgga taatgaaacc atgcggcgcg ggaaaagggc cagggatttc gacgcggtaa
     3121 tcgccgcacc cttcgggcgg gtcggtttca tcctggatgg cgatgcggtc acggacatca
     3181 gctttctgga caaaaacaca ccactcttgc cgccgcgcag cgcacaagcc cgtaaagtat
     3241 cgcgcgtact gcggtcgtat ttcaccaatc ccaaggtttc ctttcgtctt ccactgaaga
     3301 cgtcaggcac ggcattccag cagcgtgtat ggcgcgcgtt gcggcgcatt cccgcgggcc
     3361 ggacgctcag ttacggcgtg ctcgcgcaaa aactcgacac ctcggcgcgc gccgtgggca
     3421 acgcctgccg aaccaatccc attcccatta tcattccttg ccatcgtgtc gtcgcctcga
     3481 atggagtggg cggtttcatg ggcaagcgct cgggctcgcc gctgaatctg aagcactggc
     3541 tgctgaagca tgagcgcagg aaataaggac ctcatcgaac gcttttgcga cgctctgtgg
     3601 ttgcaggaag ggctgagcgc caacacgctc gaagcctacc ggcgcgatct ggaagggctg
     3661 gaccgctgga ttacgaaacg tggcacggcg ctgatgtcgg cctcgcgcgc cgacctgctg
     3721 gaatatcttt caagcaatgt cggcaagggc gcccgcccgc gtaccacggc gcggctgctt
     3781 tccagcctgc ggcggtttta cggttatctg gtgcgcgagg aaaaaatcaa aaccgacccc
     3841 acggcgctga tcgactcgcc caagcttggc cggcctttgc ccaaatcgct gagcgaagag
     3901 caggtggaaa aactgctgga atcgccggac atcaaaacat ctttggggct gcgcgaccgc
     3961 gccatgctgg aaacgctcta cgccactggc ctgcgcgtgt cggaactggt ggggctgacg
     4021 ttgtcgcagg tgagcctcac gcagggcgtg gtgcgcgtca tcggcaaggg agacaaggaa
     4081 cggctggtgc cgctggggga ggaagccatc gcgtggatca gccgctatct ggccgaaggc
     4141 cgtcccaaac tcgtcaaaag ccgctcgacc gatgcgctct tcaccaccgc ccgcggcggc
     4201 ccgatgaccc gccaagcctt ctggcacaac ctcaagaccc acacccgcaa ggccggcatc
     4261 gatgccaaaa agctgtcacc ccacaccctg cgccacgcct tcgccacgca cctgctcaac
     4321 cacggcgccg acctgcgcgt cgtccagatg ctcctgggtc atgccgacct ctccaccacg
     4381 cagatttaca cgcatgttgc gcaggcaagg ttgaagacgt tgcacgaaca acatcatcca
     4441 aggggatagt gtcaggtggg attgtcttct ttaagacttc tataaaactc aagagatggc
     4501 ttgagttctc tgatttcata ctgctcccat gaccacttcc caagtgacat gtctcgaaaa
     4561 tactgatatt cgattcgtgc catgcgctta atctccctat caatgtgttc tttttctgtc
     4621 tctgctagtc gatcgcaacc aagcagttct tctttgaaga atggattttt ttcaagaaat
     4681 gacaaatgcg tatgccgacc ttttgttgga ttgttacgtg gaaagaactc taggccaaaa
     4741 tacattagct gatggttgag agccgcttta ttttttggga atcgatcttc aatgctgcca
     4801 cgagtaataa ctgcgtggca tgagttgcag atatgcagca gattagaagc atcgtaggtt
     4861 ccgccatcgc atgaccaaat acagtgatgg acttcgaact cccgtctctt acagaggcaa
     4921 atagggcaat agtcattcgt caaccagcaa ctatcctggc cggcctgaaa ttctgtatct
     4981 ctcccaagat aaagccccgt gttgggcttg tcttgagttg cagccttcca agcggagagg
     5041 agccattcat gctcctcttt gctcatgtac agtccacatg ctatgccctc aattacaggg
     5101 tattcctccc agcttacgtc tgagccttta acaatagctt tttctagttc gctgttgtat
     5161 tcgaactccc aatcttcgcc atatttattg gtaaaagtag aacggatcat atgttaatca
     5221 gaaattcgat ctcgaccgaa ctaaattgga cttcgatttc gccgcatagc ttggaattta
     5281 gaccccaacg tgattaattt gcttttaagt aatctggcta cttgattgcg caagtaacgt
     5341 tagaagttta tttaatcact atggccgata gtggtaacta tagttactgt agttcctttt
     5401 ttcagtttct tggaattttt ctgggaatga taatgacgga tagcgttgac aacttcgtca
     5461 cgagaaagct ttctgtatgc gattacattg tatgtgacgt ctttttcttt attcctgatt
     5521 gagttgctta tatgcggctg ctccattttg acactccttt atatatacga ggaacaacag
     5581 atcagagtac ttgaaaatgt gtagttagag ttttgttaat ggatctattt ttcatgtact
     5641 ctgacccctt ggttttccaa gcctactcac tgccgctatt cgccaatcac ccaaactatc
     5701 tgttggctca aacatagttt tgcgctttaa agaagaataa aaaacttaga tggcaattta
     5761 tggaatcttg cctttccaag actgataatc attgtgaaaa agttcgatgt tatctccaga
     5821 agccaaagtc catccttcca tgtgaccgat ctcagacatt ccttgaggag ctacatcaag
     5881 acggtagttc tttccttgtt gcatggaagg attttttagc gtgaccaata ttgagagatg
     5941 ccgggtagat ttatttttta cttgcagcac caaaccaggt cctaataagg ctccacgaaa
     6001 ggcgacttct accggcatag gtggaagact ttgttcctga aattcgcgag attggaaata
     6061 ccagacaatg ccaccgatta ttaggagtaa cgccacccac gcagctggtg aagttttctt
     6121 cttttccttg cttggagctg tcgctgttgt tggccccttc ataggggctc cacaattggg
     6181 gcatgtaaca gcttgatcag agatttgatg tttgcactct gggcactgca ctaaagccat
     6241 aagccatccc tccctaaact ttcgccacag aatctaggct atcgttttta actacgccat
     6301 gtttgtgctt tccaactctt gagtcaagcg aagccctgaa agtgctgatt ttttcccatc
     6361 ccccctaagg cgagccgaac accgaggaca gccgaagcgc gggcccgcag gggcgagcga
     6421 gggatcgtga gcgtgcgcat ccggcgcaag gaacgcgacg tatgcgcacc ccctgaggca
     6481 accgaggtgt gagggaaccc gagcggggtt ggcgagggtg aagccttggg ggcggccttt
     6541 tcttttgtcc atttcttttg gccgaacaaa agaaatggac cccagggtgc aggggcggga
     6601 gccacccgca attagctttt gaatcgcggg cgcagcccgc tcgacagaaa gaaaaaagga
     6661 aatttcgtgc gcggagcgca cgaaacctga tcaatgataa gccgaccgat taatctgctc
     6721 gtgatcaata tccacatcaa acacctccac ccccggtacg cgcttgtagc cgaggacgag
     6781 ggcgagcttg tggatcaggg tcttccagtt gagggaattg acgcggcggc gcaggacgtc
     6841 gttgacctcg agcgtcagtt tttcatgctg cttttccagc gccggcagct tgtggaaatt
     6901 gcgcagcgcg aaccaggcgc acagcgctgg cagtagcttg atgaccaagt gcgcgcttgg
     6961 cgtcgctagc cagaagacaa tgaaactgac gccggcgaag acgaaaaaca ggccgcgacg
     7021 gacagccctg gcgtagtcgt tgcggttgcg ttccatgctc tcgcgcagtt gtcgttcggc
     7081 gtcgcgcgag gcgatggagt gggcgtaatg gatgtcgcgg aacatgctca gtatcttgag
     7141 catgcgctcg ctgtagcgcc ggttctcgat ggcgaatgag tccagcgccg attggaggct
     7201 ttcacggaac tggcggtagc tcggcgtgac tggcggttcc cggtaatggt cgacggcgtg
     7261 gttgatcagg tagcgcagtt ccagcacatg cgcgatcttc atcggaaagc gcaggttgat
     7321 gaacttggtg tagaacgtgt gaaaaccctc cggcgtcagg ctctcgccgg agaaatcttg
     7381 tgccagctcc cgttggcgtg ccctttcctt cctgcgtttg ctcatttttc ttgtcatgga
     7441 tggaaaaaac cgttcaattt cccgccccgc tatcacgata atgaaccatc ccttagttct
     7501 aatgggggaa aggataacgg ataaagcagg ggttttctac ccgccggggc ggggttccgc
     7561 gggtgcggat tagggtctca aaagcaaaag aatgaatatg attttgaacc gccaagacgc
     7621 caaggtcgcc aagaaaatat ctgttttttg aagcaaaaac ttggcgttct tggcgtcttg
     7681 gcggttaatt ttctttttta gggtgttttc ttagctgtta cccagcatgt tgcgcaggtt
     7741 agcgaggtaa acgtcggcgc ggccgagcat ggcctgcggg tccggctccc ggtccggctc
     7801 ctcccatttg aggtcgtccg ccggcatttc cttgaggaag cggctgggct cgcagacgag
     7861 gacctcgccg ccgcgcttgc gccggtcggc gtaggagaag gtcagtgtct ggcgcgcgcg
     7921 cgtcatggcc acgtaggcga ggcggcgctc ttcctccacg cccgcgtccg actggctctg
     7981 gtgatgcggc agcaggcctt cctccatgcc gacgatgaag acatgcggaa actccagccc
     8041 cttggcggcg tgcaccgtca tgagcgacac cagatcgccg gggttttcct ggtcgccctt
     8101 gtccaggatg ccggagaggg tcaggcgcgc caccaagtcg ctcagcgtca gatcttcttc
     8161 ctggcgcgcc aggcgctgga tccagctcac cagttccagc acgttttcca tgcgccattg
     8221 cgcgatcttg tgatcgttgc aggtgtcctt gagccattcc tcgtaatgca ggttggccag
     8281 catgtcgcac acgagcttgc ccggctcttc atcttccgac ttggtgatca tttccatcag
     8341 ccagtgcgtg aaactgcgca gcgtcgtttc ctggcgcgcg gtgagtttgg tgtcaatgcc
     8401 gggatccatg gatgcctgca acaggctggt gttaagttcg gcggcgtgcg cggcgagctg
     8461 ctccagcgtg gccgggccga tctcgcgccg cggcgtgttg atgacgcgca ggaaggcgtt
     8521 gtcatcgccg gggttgcaca tcaggcgcag gtaggccatg acgtccttga tctcggtctt
     8581 gtcgaagaac gaagtcccgc cgctcaggaa gtaggggatg cgccgctcgc gcagcacgcg
     8641 ttcgagcacg cgcgactggt tgttctcgcg gaacaggatg gcgtaatcgc gaaaatcggt
     8701 gttgtgcttg aatttgtggt acagcagctc ggagacgacg cgctcggcct cgtgctcgtc
     8761 gccgcgcgcc cgcagcacgc gcaggggcac gccgtaatcg cggtcgcacc acaacgcctt
     8821 ctcgaaaata tgcgggttgt tggcgatgag cgcgttggcg gccttgagga tacgcccgct
     8881 cgaacggtag ttctgttcga gcttgatgac tttcagcatc gggaagtcct gttgcagctg
     8941 cttcaggttc tcggggtgcg cgccacgcca ggtatagatg gactggtcgt cgtcgcccac
     9001 cacggtcagc gccccgcgat ggccggccag cagcttgacc agctcgtact ggcacaggtt
     9061 cgtgtcctgg tattcgtcca ccagcaggta acgcacgcgc cggcgccatt cctccagtat
     9121 ctccggctgg ttctggaaca ggcgcaccgg cttgaggatg aggtcgtcga ggtccatggc
     9181 gttgtaggcc tggaggtgac gctcgtattc gccatagacc cgtgccgcca cgtcctgaat
     9241 ggactcgccg ccggccgcga gcggcgcttc cgccggcgag atcagattat ttttccacat
     9301 ggagatctgg tggcgcacgg cctcggcggg attgttcttg ccggagtatt gcccgcgcag
     9361 cagtttgctc agcagcgcct ggctgtcctc gccgtcgtac agcgagaagc ccggccggta
     9421 gccgatctgg tccagatgtt cgcgcaggat tttcaggccg aaggtatgaa aggtcgagat
     9481 cagcagctct ttgctgtagt cgccgttcat aaggtcagcc acgcgcgact tcatctcgcg
     9541 cgcggccttg ttggtgaagg tgacggcgac gatattgcgc ggggcgatgc cgtagtcgcg
     9601 gatgagccag gagatcttgc gggtgatgac ggaggtcttg ccgctgcccg cgcctgccag
     9661 taccagcagg gggccattga tgtactgcac ggccgctttc tgttgcgggt tcagtttggg
     9721 caattccgct ggatcgtgat tggaaatgtc ataactggac cgacattcta acctcatttc
     9781 cccgcggttt gaaagatgaa tttccggaaa aatttttatt cacgccagtt gttcaagaaa
     9841 atttttcatc agctcccgcg ccgcggacgg tttgtcaagc agccggccgg cgagatcgaa
     9901 caggggatgc ggacgcgggt cgaacagccg gtgagtgcgc accatcttca gggtgtgcac
     9961 gccttcgcct tcgagcgccg cggtttcgcc gcgcaccagc cggcatccca gctcgtgatg
    10021 gcgggaacct tggctcgtgg cggtggtaat caggtcgccg agaccggcga gccggtgtgc
    10081 cgcgcctgcc gaaccgccca gaccggcgac gatacgctcc agctcgtgca tggctgcggt
    10141 ggcgagataa ccccgcatgt tgtcgcccag gcccagctcg tctgccacgc cgaacaggat
    10201 ggcgtagacg ttcttgagca cggccgacca ggaaatgccc tcgatgtcgg tggtgtgttc
    10261 gaggtacagc gacgtgccgg caaacagatc gcgcaaccgg tcaaagcttt cggcccgcgc
    10321 ggcgcctgcc tgcgcgaagg ccgga
//
LOCUS       DBNGZE010000287         8627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_40294, whole genome shotgun sequence.
ACCESSION   DBNGZE010000287 DBNGZE010000000
VERSION     DBNGZE010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_40294"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC16FV_07440"
     CDS             <1..1010
                     /locus_tag="AC16FV_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR2849925.1"
                     /translation="SCPHDAISLVFGTAKRGMDIPQVNPSFETNVPGLYIAGELGGIG
                     LIRKAVEQGRQAMESIRKKKTGNAPLDVVVIGAGPAGIAASLGALEHKLKFVTVEQED
                     SLGGTVYHYPRNKIVMTAPMQLPILGKVRMSEISKEALMEFWQGVVIKTGLKINFSER
                     METIARNGSGFTVKTSRGSYNTRTVLLAIGRRGTPRKLEVPGEELPKVVYRLIDPEQY
                     RNQQVLVVGGGDSALEAALALADEPGTTVTLSYRGEAFGRVKEKNRQRLQETEKKGRV
                     KVLLQSNVKKIERDKVTLDRAGKMIEIKNDIVIVSAGGVLPTPFLKEIGVLVETKYGT
                     A"
     gene            complement(1018..1557)
                     /locus_tag="AC16FV_07445"
     CDS             complement(1018..1557)
                     /locus_tag="AC16FV_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR2849926.1"
                     /translation="MKKYIVPLAIFAVIGGLLFYALYLSNTGRYSPKDIPSPLIGKAL
                     PAFSLSTVADPARKVSRDDMLGRVYLLNVWASWCVACRQEHPFLNELARNKAVTIIGL
                     NYKDKREDALGWLGGLGNPYELSLADGDGRLGIDLGVYGVPETFVIDKQGVIRYKQIG
                     PITPEAWEQKLAPMIKELS"
     gene            complement(1554..2012)
                     /gene="ccmE"
                     /locus_tag="AC16FV_07450"
     CDS             complement(1554..2012)
                     /gene="ccmE"
                     /locus_tag="AC16FV_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR2849927.1"
                     /translation="MKTRHKRLAIVISGVVGLTVAGLFVLNAFRSNLVFFYSPTEVLE
                     GKVAIAAGKTIRIGGLVEQGSVKRSSDGLKVDFIVTDLNKKLPVHYEGILPDLFREGQ
                     GVVAQGRLKDGNQFVASEVLAKHDEKYMPPEAAQALKGGTFTPGNEAKKP"
     gene            complement(2009..2179)
                     /gene="ccmD"
                     /locus_tag="AC16FV_07455"
     CDS             complement(2009..2179)
                     /gene="ccmD"
                     /locus_tag="AC16FV_07455"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR2849928.1"
                     /translation="MNWTEFFAMGGYGQYVWGSYGLAALILILNLYLPLRRRKTVRRL
                     LREFLRLKEQTR"
     gene            complement(2176..2904)
                     /locus_tag="AC16FV_07460"
     CDS             complement(2176..2904)
                     /locus_tag="AC16FV_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIR2849929.1"
                     /translation="MHKYASPKFFYTLAGQLIPWTAALTAVLLLAGLYLGLFVAPADY
                     QQGESYRIMFIHVPAAWMSMFIYMLMAGAGAVALVWNIKLAEIFASSCAPIGASFTFL
                     ALVTGSLWGKPMWGTWWAWDARLTSELILFFLYIGYMALQASIDDARRAARAAAILAL
                     VGVVNIPVIHFSVEWWNTLHQPASVSKIGTPSIHPSMIAPLLLMAFAFTFYFVAVALL
                     RMRNEILERERHTTWVADMLGSKA"
     gene            complement(2907..3563)
                     /gene="ccmB"
                     /locus_tag="AC16FV_07465"
     CDS             complement(2907..3563)
                     /gene="ccmB"
                     /locus_tag="AC16FV_07465"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR2849930.1"
                     /translation="MARLFWRDLTVAMRRSTDILTPLVFFVIVVSLFPLGLGPEPAVL
                     RTIAPGVIWVSALLATMLSLNRLFANDHADGTLEQMALAPYPLALLALTKIAAHWTLT
                     GLPLVLISPLLAVQMHLPDSAISTLVLSLLLGTPILSLLGAVGAALTLGLRGGGVLVS
                     LLVLPLYTPILIFGAGSVAGVIAGIDIEAHFSLLGAFLALALTFAPWAAAAALRVSLD
                     "
     gene            complement(3586..4197)
                     /gene="ccmA"
                     /locus_tag="AC16FV_07470"
     CDS             complement(3586..4197)
                     /gene="ccmA"
                     /locus_tag="AC16FV_07470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HIR2849931.1"
                     /translation="MLEAVDLECVRGDRPLFSHLSFSLKPGELLHVTGANGSGKTTLL
                     RTLCGLTRAHAGEVRWNGSAINKLGDDYHAQLAYVGHANGIQGDLTPVENLRVASSLS
                     GDADKSRIGTTLERLGLAAYHHFPSKILSQGQKRRLALARLVVEHKPLWILDEPLSGL
                     DVDSVSLMTTILMEHLAEGGLIVITSHQDVDVKTKTVLQIRIS"
     gene            complement(4244..4319)
                     /locus_tag="AC16FV_07475"
     tRNA            complement(4244..4319)
                     /locus_tag="AC16FV_07475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4284..4286),aa:Arg,seq:cct)
     gene            complement(4415..4490)
                     /locus_tag="AC16FV_07480"
     tRNA            complement(4415..4490)
                     /locus_tag="AC16FV_07480"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4455..4457),aa:Ala,seq:cgc)
     gene            complement(4606..5625)
                     /locus_tag="AC16FV_07485"
     CDS             complement(4606..5625)
                     /locus_tag="AC16FV_07485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2849932.1"
                     /translation="MRPKTRLSNQSALLNTAVLLPCHNEGPVIAEVIKAFRAALPEAD
                     IYVYDNNSTDDTAANALAAGAIVRRESMQGKGHVVRRMFADVEADIYVLADGDGTYDA
                     NIAPHMIDLLRNDNLDMVVGTRAQENNPRSYRFGHVFGNRFLTATVSKLFSSRFHDIL
                     SGYRVMSRRFVKSFPALASGFEIEAMLTIHTLDLRLPHAEVECAYFERQESTASKLNT
                     LRDGARILGTIMLLYKEFHPFRFFGYLSGLLVLTSLALGIPVVIEYLETGLVPRVPTA
                     ILASGLMVLAGIGLTCGLILDSVSRERREIKRLHYQSFPSIQEVVTESTARLGASGMV
                     SRNKP"
     gene            complement(5629..7002)
                     /locus_tag="AC16FV_07490"
     CDS             complement(5629..7002)
                     /locus_tag="AC16FV_07490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR2849933.1"
                     /translation="MQNIPGGVKQLLGRGIHIPRYVYVLLVILLVAFDLRIQSVVNSV
                     VDTPVRADAREYVLYAHNLKNFGIYSRSDTFAADGQANLVPDAKRAPLYPLFIALFLD
                     NQPTADNVATITRIQAVISTFTVLLVFWVARCFLPIPLALAASALTAISPHLVTMNIY
                     LLSETLFSFFVVLATLLLSKSAEKPGAVIPVFAGLVLGASALTHPMLLYFVVPLVFFL
                     LYSWGWNSGWKKVAAIVLGFSVLYGPWIARNIDTLGAPGDNTLMLAALRTGVYVDMMY
                     QKDPQSYGYPYRFDPNFEKTSGDLSSVLTEVGNEFRKAPAEQLRWYIFGKPLTLWSWS
                     NIEGPGDVFVYPVLSTPYRYLPQFKVTHALMQSIHWMLVALMAAGICIAWLPSRWSGL
                     SNEALFVARAISILLLYHTAIMIVGFPLPRYAIPMRPFLHIMSMLPVALAVRWVLEKQ
                     SAQTRPG"
     gene            complement(7063..7710)
                     /locus_tag="AC16FV_07495"
     CDS             complement(7063..7710)
                     /locus_tag="AC16FV_07495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2849934.1"
                     /translation="MFIKIPPADLVRLTDARHERNMYAHPNPVARGIFWQRLEVGYRL
                     LDDVPAASKVLDFGGGSGAFLPALAQRFADVSVVDRDLNDARHVAAHYQLKNARLFEQ
                     DITNWDSTETYDLVVTMDVLEHFADKSVPRRFLDGRLKPGGLLLVSLPTENWLYRLGR
                     AILRKTKPTDHYHPAHELVQYYREAGYTLLAHRYVPRLGPLALPLFYIVLFRKSR"
     gene            complement(7718..8455)
                     /locus_tag="AC16FV_07500"
     CDS             complement(7718..8455)
                     /locus_tag="AC16FV_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR2849935.1"
                     /translation="MSTHLDLHDRTLGRVYRRAYFAYKRILDRHLIASARKLAKPGTL
                     VLDVGANIGFFSITLARLSGISLLAFEPDRQNFQSLGKSIAEYALGSRIYPYMIALSD
                     TTGTGSLYLSDLAPTDHKLINTRSSRSIEIALARLDDFFSQHVDLAKTPVSLIKIDVQ
                     GAELLVLRGMHQTLRANNYPPILVEYSPDDLIQAGVSPVDFFGAFETLGYRPHTLPEL
                     KPRHPDWFIHNTRGAYRDLAMVHSTAS"
BASE COUNT         1974 a         2381 c         2641 g         1631 t
ORIGIN      
        1 cctcctgccc gcacgacgcc atctcgcttg tgttcggcac cgccaaacgc ggcatggata
       61 tcccccaggt caacccaagc ttcgaaacca atgtcccggg cctctacatt gccggtgagc
      121 tgggtggcat agggctcatc cgcaaggcgg tggagcaggg gcgtcaggcg atggaatcca
      181 ttcgcaagaa gaagacgggt aacgcaccgc tggatgtcgt cgttatcggc gccggcccgg
      241 cgggcatcgc ggcctcgctc ggcgcgctcg agcacaaact gaaattcgtc accgtggagc
      301 aggaagactc gctcggcggc acggtgtacc actatccgcg caacaagatt gtcatgaccg
      361 cgccgatgca gctgccgata ctcggcaagg tgaggatgag cgagatcagc aaggaagcac
      421 tgatggaatt ctggcagggt gtggtcataa aaaccggcct caagatcaat ttcagcgaac
      481 gcatggaaac catcgccagg aacggaagcg ggtttaccgt gaaaacctcg cgaggctcgt
      541 acaataccag gacagtatta ctggcgatcg gccgacgcgg cacgccacgc aaactcgagg
      601 tgccgggaga ggaattgccg aaggtggttt atcgcctcat cgacccggag caatatcgca
      661 accagcaggt gctggtggtg gggggcggcg acagcgcgct ggaagcggcg ctggctctgg
      721 ctgatgaacc tggaaccacc gtgacgctct cctatcgcgg cgaggccttc gggcgtgtga
      781 aggagaagaa tcgtcagcgc ctgcaggaga cggagaagaa aggccgggta aaggtgctgc
      841 tgcaatcgaa cgtgaagaaa atcgaacgtg acaaggtcac gctcgatcgc gccggaaaga
      901 tgattgaaat taaaaacgat attgtcatcg tcagcgccgg tggcgtgctg cccacgccgt
      961 ttctgaagga gattggcgtg ctggtggaga caaagtacgg gacggcgtga ttcggaacta
     1021 agacagttcc ttgatcatcg gcgccagttt ctgttcccac gcctcgggcg tgatcgggcc
     1081 gatctgcttg tagcggatga cgccctgctt gtcgatgacg aaggtttcgg gtacgccata
     1141 gacgccaaga tcgatcccca gccgcccatc accatccgcg agactcagtt catacggatt
     1201 acccaggccg ccgagccagc cgagcgcatc ctcgcgtttg tccttgtagt tgagaccgat
     1261 gatggttacc gccttgttgc gcgcgagttc gttgagaaac gggtgctcct ggcggcaagc
     1321 gacgcaccac gaggcccaga cattgagcag gtaaacccgc cccagcatgt cgtcacggct
     1381 gactttgcgc gccgggtcgg cgacggtgga gagggagaac gcgggcagcg ccttgccgat
     1441 caatggcgat ggaatgtcct tcggtgaata gcgccccgtg ttggatagat agagggcata
     1501 aaacagcaaa ccgccgatca ccgcgaagat tgccagcggg acaatatatt ttttcatggc
     1561 tttttcgctt cgttgcccgg ggtgaaggtt ccacctttca gggcctgcgc cgcttccggc
     1621 ggcatgtatt tctcgtcgtg cttggccagc acttcgcttg ccacgaattg gtttccgtcc
     1681 ttcaggcggc cctgcgccac cacgccctgg ccttcgcgga acaggtccgg caggatgcct
     1741 tcataatgca ccggcagctt cttgttgaga tcggtgacga tgaaatcaac cttgaggcca
     1801 tcggatgaac gtttcaccga tccctgctcc accaggccac cgatgcgaat ggttttgccg
     1861 gcggcaatgg cgaccttgcc ttccagcact tccgtgggag agtagaagaa cacgaggttg
     1921 ctgcggaagg cgttcagcac gaacaatccg gcgacggtaa gtccgacgac accggaaatg
     1981 acaatggcca gacgcttgtg tcttgtcttc atcgggtttg ttccttgagg cgcagaaatt
     2041 cacgcaacag gcgccgcact gttttgcgac ggcgcaacgg caaatacagg ttcaggatca
     2101 ggatcagcgc ggcaagaccg taggagcccc acacatactg gccgtagccg cccatggcga
     2161 aaaattccgt ccagttcatg ctttggatcc caacatgtcg gccacccagg tggtgtggcg
     2221 ttcgcgctcc aggatttcgt tgcgcatgcg tagcagcgcc acggcgacaa agtaaaaggt
     2281 aaacgcgaag gccatcagca gcagcggcgc aatcatgctt gggtgaatcg agggcgtgcc
     2341 gatcttgctc accgaggctg gctggtgcag ggtgttccac cattccacgg aaaaatgaat
     2401 gacgggaatg ttgaccacgc ccaccagcgc cagaatagcg gcggcacgcg ccgcgcggcg
     2461 ggcgtcgtca atactggcct gcaatgccat gtagcctatg tacaggaaaa acaggataag
     2521 ttccgaggtc aggcgcgcgt cccacgccca ccaggtaccc cacatgggct tgccccacag
     2581 cgagcctgtg acgagcgcca ggaaggtgaa ggaggcgccg atcggcgcac agctgctggc
     2641 gaagatttcc gcgagcttga tgttccagac gagtgccacc gcgcccgcgc cggccatgag
     2701 catgtaaatg aacatggaca tccatgccgc gggcacatgg atgaacatga tgcgataact
     2761 ttctccctgc tggtaatccg ccggcgcgac gaacaggccg agataaagac ccgccagcag
     2821 cagtaccgcg gtgagtgcgg cggtccacgg aatgagttgc ccggccagag tgtagaaaaa
     2881 tttgggtgag gcgtatttat gcatggtcag tccagagata cgcgcagtgc cgccgccgcg
     2941 gcccagggcg cgaaggtcag ggccagcgcg agaaaggctc ccagcaggga aaaatgcgct
     3001 tcgatatcga tgccggcaat gactcccgcc acgctgccag cgccgaaaat cagaatcgga
     3061 gtatacaacg gcaataccag cagagaaacc agcacgccgc cgccgcgcag ccccaatgtc
     3121 agcgccgcgc cgaccgcgcc aagcaggctc aggatcggcg tgcccagcag cagcgagagc
     3181 acgagcgtgc tgatggcgga atccggcaaa tgcatttgta ccgccagcag gggggagatc
     3241 agcaccagcg gcaatccggt gagcgtccag tgggccgcga tcttggtcag cgccagcaac
     3301 gccagcggat agggcgccag cgccatctgc tccagggtgc cgtcggcgtg atcgttggcg
     3361 aacagacgat tgagcgagag catggtcgcg agcagcgccg agacccagat aacgccgggc
     3421 gcgatggtgc gcaacacggc aggctcgggc ccgagcccga gcggaaaaag actcacgacg
     3481 atgacgaaaa aaaccagcgg cgtgagtatg tccgtgctgc ggcgcatggc aaccgtcagg
     3541 tcgcgccaga acaggcgggc aatggcttgc aataatttcg gcacgctagc tgatccggat
     3601 ctgtaatacg gtttttgttt taacgtcgac atcctgatgc gatgtgatga cgatcaatcc
     3661 gccctccgca aggtgctcca tcaggatggt cgtcatcagg gacaccgagt ccacgtcgag
     3721 cccgctcaag ggttcgtcca gaatccacaa tggtttgtgt tcaaccacca gccgcgcgag
     3781 cgcgagccgg cgtttctggc cctgcgacag gattttggaa gggaaatggt gatacgccgc
     3841 gaggccaaga cgttccagcg tcgttccgat tcgcgatttg tcggcatcgc cgctcagact
     3901 ggaagccacg cggagattct caaccggagt cagatcgcct tggatgccat tggcatgccc
     3961 aacgtaggcg agttgggcgt ggtagtcgtc gccgagcttg ttgatggcgc tgccgttcca
     4021 gcgcacctcg ccggcgtggg cacgcgtcag gccgcaaagc gtgcgcagca gggtagtctt
     4081 gccgctgccg ttggcgccgg ttacgtgcag cagttctccc ggtttgagag aaaaggacag
     4141 atggctgaac agaggccggt caccgcggac acattccaga tcgactgctt ccagcaaaga
     4201 cggcctccct caggcaggtt gaagatgcat caattcttga acatggtgcc ccaggccgga
     4261 gttgaaccgg cgacctgccg cttaggaggc ggctgctcta tccactgagc taccggggcc
     4321 cgccgctatt ctagcagcag gacagggcaa aaccgtccgg cgggaaggcc gggccgtaaa
     4381 tgaaaatggc cggaatttcc ggccatggcg ggattggtgg agcggaggag gatcgaactc
     4441 ccgaccttcg cattgcgaac gcgacgctct cccagctgag ctaccgcccc aaaaaggatg
     4501 ttgaggtaca gaaatgcttc ctcaaccaag gtatcaataa atcagggcgg caattctaaa
     4561 cacagccccc cgctgaaaca agacattatg ccggaattcg gcgtttcacg gtttgttccg
     4621 cgagaccatg cctgaggcgc cgagcctggc agtgctttct gtgaccacct cttgtatgga
     4681 aggaaaagac tggtagtgca gtcgcttaat ttctcttcgt tcacggctta ccgaatccag
     4741 tatgagtcca caggtcagcc cgattcccgc caataccatc agcccggagg caagtatcgc
     4801 cgtcgggacg cggggcacca gcccggtttc gaggtactcg ataacgaccg gaatgcccaa
     4861 tgccagggaa gtcagcacga gcagacccga gagataaccg aaaaaacgga agggatgaaa
     4921 ctccttgtac agcagcatga tggttccgag aattcgcgcg ccgtcacgca gggtgttgag
     4981 tttgctcgcc gtgctttcct gcctctcaaa ataggcacat tcaacctcgg cgtgaggaag
     5041 gcgcagatcc agcgtatgaa tggtaagcat cgcttcgatc tcgaacccgc tggcgagggc
     5101 cgggaaagat ttcacgaagc ggcgcgacat gacccggtag ccggaaagaa tgtcgtggaa
     5161 ccggctgctg aagagcttgc tgaccgtggc cgtcagaaag cgattgccga agacatggcc
     5221 gaaacgatag cttcgaggat tgttttcctg cgcgcgcgtt ccgaccacca tgtcgagatt
     5281 atcgtttctc agcagatcga tcatgtgagg ggcgatattc gcgtcatagg tgccgtcgcc
     5341 atccgccagc acgtagatgt cggcttccac gtcagcaaac atgcggcgca ctacatggcc
     5401 tttgccctgc atggattcac gccggacgat ggcgcccgcg gccagggcgt tggctgctgt
     5461 gtcgtcggtg gagttgttgt cgtacacata aatgtctgct tccggcaatg ccgcgcggaa
     5521 ggccttgatc acctcagcga taaccggacc ttcgttatga cacggcaaca acaccgctgt
     5581 gttcagcagg gcagactgat ttgagaggcg ggtcttgggg cgcacgattc agccgggtct
     5641 ggtctgggca gactgctttt ccaggaccca tctgacggcc agcgcgacgg gcaacatgga
     5701 catgatgtga aggaacggac gcatgggaat ggcgtaacgc ggcagcggga agccaacaat
     5761 catgatcgct gtatgataca gcaaaagaat cgagatggcg cgggcgacaa agagtgcctc
     5821 attcgaaagc cccgaccagc gcgacggcag ccaagcgata catatccccg ccgccatcaa
     5881 tgcgacaagc atccagtgaa tggactgcat cagcgcatgc gtcaccttga actggggcag
     5941 atagcgatat ggcgtgctca gcacgggata tacaaagaca tcgcccggcc cctcaatatt
     6001 gctccatgac catagcgtga gcggtttacc gaagatatac cagcgcaatt gctcggctgg
     6061 cgcctttctg aactcgttgc caacttccgt gagcacggaa gacaaatccc cggaggtttt
     6121 ctcgaaattg ggatcgaagc ggtatggata accgtaggat tgcggatcct tctggtacat
     6181 catgtcgaca tacacgcccg tgcggagcgc cgccagcatc agcgtgttgt caccaggcgc
     6241 acccagggtg tcgatattgc gcgctatcca tggcccgtac agaacgctga atcccagaac
     6301 aattgcggca acttttttcc atccggagtt ccatccccag ctatacaaaa ggaaaaacac
     6361 cagcggcacg acgaaataga gcagcatggg atgtgtcagc gccgacgcac cgagcaccag
     6421 ccctgcaaac acaggtatta cggccccggg tttttctgcc gatttgctca acaagagcgt
     6481 tgccaagacg acgaagaaag agaagagtgt ctcgctcagg agataaatgt tcatcgtgac
     6541 caggtgcgga ctgatggccg tcagggccga tgccgcgagg gccagcggta tcggcaggaa
     6601 acaacgggca acccagaaca ccaacagcac ggtaaacgtg ctgattacag cttgtatccg
     6661 ggtgatggtg gcaacattat cggcggtggg ctggttgtcc aggaatagcg caataaacaa
     6721 tggataaagc ggcgcgcgtt tcgcgtccgg tacgaggttc gcctgcccat cggctgcgaa
     6781 tgtgtcggag cgcgaatata tcccgaagtt tttcaggtta tgcgcataca ggacatattc
     6841 gcgcgcgtcg gcgcgtaccg gcgtatccac cacgctatta acgacggatt ggatgcgcag
     6901 atcgaaggct accagcagga tcaccagaag cacataaacg tagcgcggaa tatggatgcc
     6961 tctaccaaga agctgtttta caccgccggg aatgttctgc attggattat tatggttggt
     7021 aagttcggtt gcccatgacc tttagtatag gatggccacc gcttaccggg atttccggaa
     7081 gagcacaatg taaaacagag gtaatgcaag cggaccgagc cgcgggacat agcggtgagc
     7141 cagcagggta tagccggcct cccggtaata ttggaccagt tcgtgcgcgg gatggtaatg
     7201 atcggtgggt tttgtctttc ggaggatggc gcgccccagg cgatacagcc agttctcggt
     7261 gggcaggctg acaagcagaa gtccgccagg cttgaggcgg ccgtcgagaa aacggcgcgg
     7321 tacggattta tcggcaaagt gttccagcac atccatggtg accacaagat cgtatgtttc
     7381 ggtgctgtcc cagttcgtga tgtcctgttc aaaaagccgt gcattcttca gctggtaatg
     7441 cgctgccaca tgccgggcgt cattcaggtc acggtccacg accgaaacgt ccgcgaagcg
     7501 ttgcgccaga gcaggaagaa aagccccgct tccgccgccg aaatcgagca ctttcgatgc
     7561 ggcaggcacg tcatcgagca ggcgatagcc aacctcgagt cgttgccaga agatgccccg
     7621 tgcgacggga ttcggatgcg catacatgtt gcgttcgtgc cgtgcatcgg taagtcgcac
     7681 cagatccgcg ggaggtattt tgataaacat gatcccttca ggaagcagtg gagtggacca
     7741 tcgccagatc cctgtatgca ccgcgcgtgt tatggataaa ccagtctgga tgcctgggct
     7801 tgagttcggg gagcgtgtgc gggcgatagc cgagcgtttc gaatgcgccg aaaaaatcca
     7861 ccgggcttac gcctgcctga atgagatcat ccggcgagta ttcaacaaga atcggagggt
     7921 agttatttgc cctgagtgtt tgatgcatgc cacgcagcac aagcagctcc gcgccctgca
     7981 catcgatctt gatcagtgag acgggtgttt tcgcgagatc gacatgctgt gaaaaaaaat
     8041 catcgagtcg ggccagcgct atttcgatgc ttcgggagga acgtgtattg atgagtttat
     8101 ggtccgtggg ggcaagatcc gacaggtaca gcgaaccggt gccggtcgtg tcggaaagcg
     8161 caatcatata gggatatatc cgggaaccga gggcgtattc agcaatggat tttcccaggc
     8221 tctggaaatt ctgtcgatcc ggctcgaagg caagcaggct gatgccgctc aggcgggcga
     8281 gggttatgga aaagaagccg atgttggcgc caacgtccag gacaagggtg cctggctttg
     8341 caagcttccg ggcggaggca atgaggtgtc tgtcgagaat tcgcttgtag gcgaagtaag
     8401 cacgccggta taccctgccc aacgtcctgt cgtgcaaatc cagatgcgtg gacacgaacc
     8461 ggtgcatcga gaccagcaga tgctggaccg gcgaattaaa cagcttccct ggcataaaga
     8521 tgatgagtgg tcaatacttg tggttgcagt tatgagtcca tgattcgatc ggctcaggca
     8581 ggcgatcagg gatcttgtaa cagtgaagtt tagtatcagc gaaaagt
//
LOCUS       DBNGZE010000288         9575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1707916, whole genome shotgun sequence.
ACCESSION   DBNGZE010000288 DBNGZE010000000
VERSION     DBNGZE010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1707916"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="AC16FV_07505"
     CDS             complement(<1..1062)
                     /locus_tag="AC16FV_07505"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR2849936.1"
                     /translation="MELSNIQNVALWGFLAAVAFGAIANKTNFCTMGAVSDWVNMDDK
                     NRLRAWFLAIGVAIIGAQLLQAGGVVDLGKSIYLTANFGWLGHAAGGFLFGVGMTLGG
                     GCGQRTLVRVGGGNLKSLVVMLVLGITAYMTLRGLLAPTRINAIEVANLDLATRNMSD
                     QGIATLVQSVTGIADMKVVRWAVAAVVGLGFTLYALASREFLKSYKNLFAGFSIGLLI
                     VAGWYITGKLGFDEFEPVRLESYTFVAPVGESVNYLMTYTGSTINFGIAAVFGVIAGS
                     FLYAVLSGSFRIETFSSRGDMVNHVIGGVLMGFGGVIALGCTIGQGVTGMSTLALGSV
                     DAAFRECAEQAAAMVHATAF"
     gene            1299..1532
                     /locus_tag="AC16FV_07510"
     CDS             1299..1532
                     /locus_tag="AC16FV_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR2849937.1"
                     /translation="MPTFDKELDTTGLTCPMPVMKAKKMLQSLSTGQVLHLMATDPGT
                     RSDIPALVGKTGDQIVETSEEGGKIHFYIKKTS"
     gene            complement(1585..1878)
                     /gene="tusB"
                     /locus_tag="AC16FV_07515"
     CDS             complement(1585..1878)
                     /gene="tusB"
                     /locus_tag="AC16FV_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HIR2849938.1"
                     /translation="MLHTVNKSPFQNSSLENCLRVAQKGDVILLLEDGVYAASAGTAR
                     SSLIEKAVKQCTVYAIDADLKARGLGNLIKDVRVASYGDFVDLVEQHPVHAWL"
     gene            complement(1888..2289)
                     /locus_tag="AC16FV_07520"
     CDS             complement(1888..2289)
                     /locus_tag="AC16FV_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR2849939.1"
                     /translation="MAATGKKVMVTVRKAPHGSIYVQESIEVMFIFATYDMDLTVVFL
                     DDGVLALHAGQDTKGLGIKGFMASLGALVDWEVTKVYVDRQSLKERNIPEDAIIVIGK
                     DEETDAPIRPKVLDSAEIAAMMTVQHSLVSF"
     gene            complement(2307..2663)
                     /gene="tusD"
                     /locus_tag="AC16FV_07525"
     CDS             complement(2307..2663)
                     /gene="tusD"
                     /locus_tag="AC16FV_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HIR2849940.1"
                     /translation="MKLSVLVLEGPYNHEASDSAYNFIQAALAKGHTIRGIFFYDDGV
                     YNATKLMDPPQDDRHISKRWSELGAKGVDMIVCVAAAKRRGITNEVLVPNIRISGLGQ
                     LASMIDECDRLVTFGD"
     gene            complement(2770..3459)
                     /gene="queC"
                     /locus_tag="AC16FV_07530"
     CDS             complement(2770..3459)
                     /gene="queC"
                     /locus_tag="AC16FV_07530"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017698801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR2849941.1"
                     /translation="MPADADAGHKAVVLVSGGLDSATVLALARDAGARCYALTFDYGQ
                     RHRVELDAARQVARQLGAADHRVVKLDIGWMGGSALTDTRIAVPQESSPGIPITYVPA
                     RNTVFLSIALGWAEVLDAPDIYIGANAVDYSGYPDCRPEFIHAFENLANLATKAGVEG
                     RGFHVHAPLMHMRKHEIIHAGSRLGVDYALTLSCYDPDASGRACGRCDACRLRAQGFA
                     EAGVADPTRYR"
     gene            complement(3443..4135)
                     /gene="queE"
                     /locus_tag="AC16FV_07535"
     CDS             complement(3443..4135)
                     /gene="queE"
                     /locus_tag="AC16FV_07535"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIR2849942.1"
                     /translation="MPPSETAAVTSRPGSSTSDRLRITEIFYSLQGEARTVGYPTVFI
                     RLTGCPLRCTYCDTEYAFYGGQWMELDEILKRVAEHAPRYVTVTGGEPLAQRPCRDLL
                     TALCERGYEVSLETGGTLDIAGIDPRVSIVMDLKTPGSGEERRNLYANIPHLARKDQV
                     KFVICDRADYEWSRAKLAEYKLDQLCEVLFSPCHGQLNPTELAEWILQDHLPVRMQIQ
                     LHKLLWNDARGR"
     gene            complement(4139..4936)
                     /gene="ybgF"
                     /locus_tag="AC16FV_07540"
     CDS             complement(4139..4936)
                     /gene="ybgF"
                     /locus_tag="AC16FV_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR2849943.1"
                     /translation="MVMRHSRRVGLWMAGILAASLLAPAWAAEAPAGGRSLPPVVEMG
                     VGGATSSSPPSREVLADLLQQLESLQAELRSLRGQVEVQTNEIERLKARQRDLLADID
                     RRVSELERRGVSAPAPEASAAPESTAPAPAAQAESAQEQKDYDAAFNFMKQGNYERAA
                     KGFRDFLAKHPQSALRENAQYWLGEAFYVMRDFRKALTEFSKLMKNHPKSLKAPDALL
                     KIGYSHYELGEWAKARENLNQVIARYPGQPAAKSAEQRLAKMKKQGH"
     gene            complement(4946..5464)
                     /gene="pal"
                     /locus_tag="AC16FV_07545"
     CDS             complement(4946..5464)
                     /gene="pal"
                     /locus_tag="AC16FV_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR2849944.1"
                     /translation="MKNRKHLVASLFIALALAGCAGEEAVKPAAEEKGAAPAQTAPAE
                     GGAAPGTALSDREKQRRAELLAQKRVHFAFDSSAIDDEARAIIEAHSAHLVANPQLKV
                     TLEGNCDERGTREYNLALGERRAQSVEKMMKVLGVAGNRIKTVSYGEEKPLCQEHNES
                     CWRQNRRAEIGY"
     gene            complement(5468..6784)
                     /gene="tolB"
                     /locus_tag="AC16FV_07550"
     CDS             complement(5468..6784)
                     /gene="tolB"
                     /locus_tag="AC16FV_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR2849945.1"
                     /translation="MRLAAKGIALLGMLLWPVFAHAVLTIEITRGVDVGTPIAIVPFG
                     WEGTTPPPHSISDVIEADLARSGRFAVLPRKDFISLPHEDKDVVFKDWRILKAEALAI
                     GSMRQAAPGKFQVQFRLYDVFKGSQIAGFRYTIGAEMLRSVAHQISDIIYEKITGEPG
                     AFNTRIAYVTREEPPGVKPVYKLQVADSDGVNPQTVVRSPEPLMSPAWSPDSKRLAYV
                     SFEDKRSKVYIQNMMDGRREMVAEFSGINGAPAWSPDGRSLALTLSRDGNAEIYAMQL
                     DTRTLRRLTRDPAIDTEPSWSPDGRDIVFTSDRAGTPQIYRMSASGGAAERLTFEGDY
                     NARASYAADGKSITLVSRNQGGFRIATLRLDNRTMQVLTETSLDESPSFAPNGRMIIY
                     ATEVRGRGVLASVSADGRVRQLFKLEEGDVREPAWSPYNRELQYKE"
     gene            complement(6800..7555)
                     /gene="tolA"
                     /locus_tag="AC16FV_07555"
     CDS             complement(6800..7555)
                     /gene="tolA"
                     /locus_tag="AC16FV_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR2849946.1"
                     /translation="MFVKKLRHNPGRVRAIVYAVLVHAAVIGLAVIGFRWTMQSPSSE
                     VVQAVVVPESPARKPEEVNKRAQEEEEARRKAEAEKRRQDELKKKQEEEQAQQRLIVE
                     RKKKEAEEKEKQKRAQETERRKEEIRRQKQTEQSLQEQLAAEEKSRQQVAQAARAATA
                     VDKYRALIRQRVSQSWNRPVGVAKGLKCVVSVRLAPGGEVLSARVVRSSGNTVFDRSV
                     ENAVFKAAPLPLPGDPTLFDNFREIEFLFSPEE"
     gene            complement(7583..7960)
                     /gene="tolR"
                     /locus_tag="AC16FV_07560"
     CDS             complement(7583..7960)
                     /gene="tolR"
                     /locus_tag="AC16FV_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR2849947.1"
                     /translation="MSEINVVPYIDVMLVLLVIFMITAPLLSQGVKVDLPQAAAQPVE
                     SQDQETLVVTVDREGKYFLDDRRISSEELRRKVAAILRLRPKTPVLIRGDRQANYGEV
                     VKAMTLLQSAGAPSVGLLTEPPR"
     gene            complement(7996..8691)
                     /gene="tolQ"
                     /locus_tag="AC16FV_07565"
     CDS             complement(7996..8691)
                     /gene="tolQ"
                     /locus_tag="AC16FV_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR2849948.1"
                     /translation="MDITTTEGIALLSLIRHASLPVQLVMLLLLAASVLSWTMIFRKW
                     FALKSANQVADRFETNFWSGTNLNHMFTEQSKKEANATGMASIFVEGFREYKRLSQET
                     RIAPMDILEGVQRAMRVALSREIDYLETHLSFLATVGSTSPYVGLFGTVWGIMNSFRS
                     LGMVQQATIAHVAPGIAEALIATAIGLFAAIPAVIAYNRYSNKVERLVTRYEIFMQEF
                     ASILQRQAYAARG"
     gene            complement(8695..9102)
                     /gene="ybgC"
                     /locus_tag="AC16FV_07570"
     CDS             complement(8695..9102)
                     /gene="ybgC"
                     /locus_tag="AC16FV_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR2849949.1"
                     /translation="MERIFSIPIRVYYEDTDAGNVVYYANYLKFMERARTEWLRALGF
                     EQDELSRREGVLFAVRSARLEFLKPARFNDLLQATVRVSRRGRASLTFAQEIRRDELA
                     LCEGEVKVACLDAATFSPRPIPENIGSKIDAGI"
     gene            complement(9214..>9575)
                     /locus_tag="AC16FV_07575"
     CDS             complement(9214..>9575)
                     /locus_tag="AC16FV_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HIR2849950.1"
                     /translation="LRRARDFAQIKGNGRLTGDIAELALSMLEVDQCGFDMLDRKLLL
                     AVIEKFSGGPVGVDNLAAAIGEERDTIEDVIEPFLIQQGYLMRTPRGRLATAMAWRHF
                     GLKAPSAPEQRGMFEAE"
BASE COUNT         1905 a         3026 c         2739 g         1905 t
ORIGIN      
        1 gaaagccgtg gcatgaacca tggcggcggc ctgctcggcg cattcccgga atgctgcatc
       61 caccgagccg agcgccagtg tagacatgcc agtaacgccc tgaccgatgg tgcagcccag
      121 cgcaatgaca ccaccaaaac ccatgagcac gccgccgatg acatgattca ccatgtcccc
      181 acgcgagctg aaggtttcga tgcggaaact tccggacagc acggcataca ggaacgaacc
      241 ggcgatgaca ccgaacacgg cggcgatgcc gaagttgatg gtggagccgg tgtacgtcat
      301 caggtaattc acgctctctc ccaccggcgc gacgaaggtg taggattcca gccgcaccgg
      361 ctcgaattcg tcgaagccga gcttgccggt gatgtaccag ccggcgacga tgagcagacc
      421 gatgctgaac ccggcaaaaa gattcttgta gctcttgaga aattccctgc tcgccagcgc
      481 gtagagggtg aagccgaggc cgacgacggc cgccaccgcc cagcgcacca ccttcatgtc
      541 ggctataccc gtgaccgatt gaaccagtgt ggcaataccc tggtcactca tgttgcgcgt
      601 cgcgagatcc aggttggcaa cctcgatggc gttgatgcga gtgggggcaa gcagcccgcg
      661 cagggtcatg taggccgtta tccccagcac cagcatcacc accagcgatt tcagattgcc
      721 gccgccgacg cgcaccaggg tgcgttggcc gcagccgccg ccgagggtca tgccgacacc
      781 gaagagaaaa ccgccggcgg cgtggccgag ccaaccgaaa ttggcggtga gataaataga
      841 cttgccgaga tcgacaacgc ccccggcctg caacagttgc gcgccgatga tagcgacgcc
      901 gatggcgaga aaccacgccc gcagccggtt cttgtcgtcc atgttgaccc agtcgctcac
      961 cgcgcccatg gtgcagaaat tggtcttgtt ggcgatggcg ccgaacgcca ccgccgccag
     1021 gaaaccccac agcgccacgt tctgaatgtt actcaattcc atgaaaaccc ccgtttgccg
     1081 ccttgatgca gaaaaatttg accatctgag acgccggtgc ccgtcgcttt attcctggcc
     1141 attcaatacc agggattatt ttgcgtcggt ttcgcgtctt gcgccatacc acctgtcaat
     1201 ccggattgtt ttatcatggt attttgacgc agccagataa cgctttcccc ggaaaaaaca
     1261 ggctagtaaa atcatttttt tagccacgag gtatgccaat gccgacgttt gataaagaac
     1321 tggacaccac gggtctcacc tgccccatgc ccgtcatgaa ggccaagaaa atgctgcagt
     1381 cactgtcaac agggcaggta ttacatctga tggcaacgga ccccggcacc cgcagcgaca
     1441 tccccgcgct ggtcggcaag accggtgacc agatcgtcga gaccagtgaa gaaggcggaa
     1501 agattcactt ctatatcaaa aaaacttcct gaaagtaaaa aaaccggttt tgcgcccggt
     1561 ttttttacgg tcctcgctgg aatcctaaag ccaggcgtgg accgggtgtt gttccaccag
     1621 gtcgacgaaa tcaccgtagc tcgcgacacg cacatccttg atgagatttc ccaagccgcg
     1681 cgccttcagg tccgcgtcga tcgcataaac cgtgcactgc ttcacggctt tttcaatcag
     1741 ggatgatctc gccgttcccg ccgatgccgc gtacacgccg tcttccagca acaaaataac
     1801 gtcgcccttc tgcgccacgc gcaggcagtt ctccagcgaa ctgttctgaa acggggattt
     1861 gttcacggta tggagcatgg aagattccta gaatgacacg aggctgtgct gcaccgtcat
     1921 catggcggcg atctcggcgc tgtccagcac cttgggccgg atgggcgcgt cggtttcttc
     1981 gtccttgccg atgacgatga tcgcgtcttc cggaatattg cgctccttga gcgattgtcg
     2041 gtccacgtaa accttcgtga cctcccagtc cacgagcgcg ccgagcgagg ccatgaaacc
     2101 cttgatgccg aggcccttcg tgtcctggcc cgcatgcagg gccagcacgc cgtcgtccag
     2161 gaacaccacc gtgagatcca tgtcgtaggt cgcgaagatg aacatgacct cgatggattc
     2221 ctgcacgtaa atgctgccgt gcggcgcctt gcgcaccgtc accatcacct tttttcccgt
     2281 cgctgccatg tcgtgcctct gcctgatcag tcgccaaacg tcacaagccg gtcgcattca
     2341 tcgatcatgc tcgccagctg gcccagcccc gagatgcgaa tattcggcac cagtacctca
     2401 ttggtgatgc cgcggcgttt ggccgcggcc acgcagacga tcatgtcgac tcccttcgca
     2461 cccagttccg accagcgttt ggagatatgc cggtcgtcct gcggcgggtc catgagcttg
     2521 gtggcgttgt acacgccgtc gtcgtagaag aagatgccgc ggatggtgtg acctttggcg
     2581 agagcggcct gaatgaagtt ataggcgctg tcagaggctt cgtgattgta ggggccttcc
     2641 agaaccagaa ccgataattt catgcgtgct ccgaaatacc tgaaacgtgt tcagtaccgc
     2701 ccgggaaact cccctcgaaa gaatagccga atatcgccaa gatggaaacg gaataccagg
     2761 cccgcgatat tagcggtagc gggtgggatc agcgacgccc gcttcggcaa acccttgggc
     2821 gcgcaggcga caggcatcac agcggccgca ggcgcgaccg gacgcatccg ggtcgtagca
     2881 cgacagcgtg agcgcataat cgacgcccag acggctgcct gcgtgaatga tttcatgttt
     2941 gcgcatatgc atgagcggcg catgcacatg aaaacccctg ccttccaccc ccgccttggt
     3001 ggccagattc gccagattct caaaggcatg gatgaattcc ggccggcagt cgggatagcc
     3061 ggaataatcg acggcattgg cgccgatata aatatccggg gcgtcaagca cctcggccca
     3121 accgagcgca atggaaagaa atacggtatt gcgcgccggg acgtaggtga ttggaattcc
     3181 cggcgaggat tcttgcggca cggcaatgcg agtgtcggtc agggccgaac cgcccatcca
     3241 gccgatgtcg agcttgacca cgcgatgatc ggcggcaccc agttgccggg cgacttggcg
     3301 cgcggcatca agctccaccc ggtgtcgttg cccgtaatcg aaagtcagcg cataacaacg
     3361 tgcaccggcg tcccgggcga gcgccagcac cgtggcggaa tcaagaccgc cggaaaccaa
     3421 caccaccgcc ttgtggccgg catcagcgtc cgcgggcatc gttccagaga agcttgtgca
     3481 attgaatctg catgcgcacc ggcaaatgat cctgcaagat ccattcggcc agctcggtcg
     3541 ggttcagctg cccgtggcaa ggagagaaca gcacctcgca aagctggtcg agtttatatt
     3601 cggcaagctt ggcacggctc cattcgtaat cagcgcgatc gcagatgacg aatttcacct
     3661 gatccttgcg agcgagatgc ggaatgttgg cgtaaagatt acgacgctct tcgccggagc
     3721 cgggtgtctt gagatccatc acgatgctca cgcgcggatc gatgccggca atatcaagtg
     3781 tgccgcccgt ctcaagcgat acctcataac cgcgctcaca caacgcggtc agcaggtcac
     3841 ggcagggtcg ttgcgccagc ggttcgccgc cggtcacggt cacataacgc ggcgcgtgtt
     3901 cagccacgcg tttcaatatt tcgtcgagct ccatccactg cccgccgtag aaggcgtatt
     3961 ccgtgtcgca ataggtgcag cgcagcggac aaccggtcag gcgaatgaac accgtggggt
     4021 aacccacggt gcgtgcctcg ccttgcaatg aataaaagat ttccgtgatg cgcagccggt
     4081 cgctcgtgga agaacccggc cgggaggtta cagcagctgt ctccgatggc ggcacaaact
     4141 aatgcccctg tttcttcatc ttcgccagcc gctgctccgc cgatttggcg gcgggctgac
     4201 cgggataacg cgcgatcacc tgattgagat tctcgcgcgc cttggcccat tcaccgagtt
     4261 cgtaatggct gtagccgatc ttgagcaggg catccggcgc tttcaagctt ttgggatgat
     4321 ttttcatgag ctttgaaaac tccgtaagcg ccttgcgaaa atcgcgcatc acgtaaaagg
     4381 cctcgccaag ccagtattgt gcattctcgc gcagggcgct ttgcggatgt ttcgcgagaa
     4441 aatcgcgaaa gcccttggcg gcgcgttcgt aattcccctg tttcatgaag ttgaatgccg
     4501 cgtcgtaatc cttctgctcc tgcgccgact cggcttgcgc ggcgggcgcc ggagcggtgc
     4561 tttcgggtgc ggccgacgct tccggcgcag gcgcggaaac gccccggcgc tccagctcgc
     4621 tgacacgccg gtcaatatcg gccagtaaat cgcgctgtcg cgccttcagg cgctcgattt
     4681 cgttggtctg gacctcgact tggccccgca gactgcgcag ctccgcttgc agcgactcca
     4741 gctgttgcag gaggtcggct agcacttcgc gcgaaggcgg ggaggaggac gtggcacccc
     4801 cgacccccat ctccaccacc ggcggcaagc tgcggccacc cgcaggcgcc tccgcagccc
     4861 atgccggcgc gagcaggctg gcggccagta tccctgccat ccacagaccg acgcgccggg
     4921 aatgccgcat gaccatcagg ccgagttagt agccgatctc ggcgcggcga ttctgacgcc
     4981 agcaggactc attgtgctcc tggcacagcg gtttttcctc accgtaggac accgtcttga
     5041 tgcggttgcc ggccacgcca agcactttca tcatcttttc cacagactgg gcgcggcgtt
     5101 cacccagcgc caggttatat tcgcgcgtgc cgcgctcgtc gcaattgcct tcgagcgtca
     5161 ccttgagctg tgggttggcc accagatggg ccgaatgcgc ctcgatgatg gcgcgcgctt
     5221 cgtcatcgat agcgctgctg tcaaaagcga aatgcacgcg cttctgcgcc agcagctcgg
     5281 cgcgtctttg tttttcacga tcggacaagg ccgtgcccgg tgcagcccca ccttcagcgg
     5341 gtgccgtctg cgccggggcg gcgccttttt cctccgccgc gggcttgacg gcttcctccc
     5401 cggcgcagcc ggcgagcgcg agagcgatga acagcgatgc aacgagatgt ttcctgtttt
     5461 tcatgtttca ctccttatat tgcagttcac ggttgtaagg cgaccaggcg ggctcacgca
     5521 catcgccctc ctctagcttg aacagctgac gcacgcgtcc gtccgcggag accgaagcca
     5581 gcacgccgcg gcccctcacc tcggtggcat agataatcat acgcccattg ggcgcgaaac
     5641 tgggcgactc gtcgagcgag gtctcggtca agacctgcat cgtgcggttg tccagccgca
     5701 gcgtcgcaat gcgaaagccc ccctggttgc gactgacgag cgtgatggat ttcccgtccg
     5761 cggcgtagga ggcacgggca ttgtaatccc cctcgaaggt cagacgttcc gccgcaccgc
     5821 cggaagcgct catgcggtag atctgcggcg tgcccgcgcg atcggaagtg aaaacgatat
     5881 cgcggccatc gggcgaccag gacggttccg tgtcgatggc cggatcgcgc gtcagccgcc
     5941 tgagcgtacg ggtatccagc tgcatcgcat aaatctcggc gttaccgtcg cgcgacagcg
     6001 tcagcgccag gctgcggcca tccggcgacc atgccggcgc cccattgatg ccggaaaatt
     6061 ctgcgaccat ctcgcgccgg ccatccatca tgttctggat atacaccttc gaacgtttgt
     6121 cttcaaaaga tacgtaagcc agacgcttgc tgtccggtga ccaggccggc gacatcagcg
     6181 gctcggggga gcgcaccacc gtctggggat tgacgccgtc cgagtccgcc acttgcagct
     6241 tgtagaccgg ctttacgccg ggcggttcct cgcgcgtgac ataggcgata cgggtattga
     6301 acgctccggg ctcgcccgtg attttttcgt aaataatatc gctgatctga tgcgcgaccg
     6361 aacgcaacat ttcggcccca atggtataac gaaaaccggc gatttgggac cccttgaaga
     6421 cgtcataaag cctgaactgc acctggaatt ttcctggcgc cgcctgccgc atgctgccga
     6481 tcgccagagc ctcggccttg aggatgcgcc agtccttgaa caccacgtcc ttgtcttcgt
     6541 gcggcaaaga gatgaaatcc ttgcgcggca gcacggcaaa cctgccgctg cgcgccagat
     6601 ccgcctcgat cacgtcggag atgctatgcg gcggcggggt ggtcccttcc cagccaaatg
     6661 gcacaatggc gatgggcgtg ccgacgtcca cgccacgcgt gatctcgatg gtcaacacgg
     6721 catgagcgaa aaccggccat aacagcattc ccagcagggc aattcctttg gcagcaagtc
     6781 gcatcaggtt ttcgttctcc tattcctcgg gactgaacaa aaattcaatt tcacggaaat
     6841 tatcaaataa ggtcggatcc ccgggcaacg gcaagggcgc ggccttgaat acggcattct
     6901 ccaccgatcg atcaaatacc gtgttaccac tggatcgcac cactctggct gacagcacct
     6961 cgccacccgg cgccagacgc acgctcacca cgcatttcaa acctttcgcc acaccgactg
     7021 gccggttcca gctctggctg acacgctggc gaatcaacgc ccggtacttg tccaccgcgg
     7081 tggcggcgcg cgcggcctgc gccacctgct gacgcgactt ctcctccgcg gccagctgtt
     7141 cctgcagcga ttgctcggtc tgtttctgcc gccgtatttc ctctttccgg cgctccgttt
     7201 cttgcgcgcg tttctgtttc tccttctcct cggcttcctt tttcttgcgt tcgacaatga
     7261 gacgttgctg cgcctgttct tcttcctgct tttttttcag ctcgtcctgg cggcgttttt
     7321 ccgcctcagc cttgcgccgc gcctcctcct cttcctgcgc gcgcttgttc acttcctcgg
     7381 gcttgcgggc aggcgattcc ggcacgacca cggcctgcac cacctcgctg gaaggcgact
     7441 gcatggtcca gcgaaaaccg attacggcca gaccgatcac cgccgcgtgc accagcacgg
     7501 catacacgat ggcgcgaact cgtcccggat tatggcgcaa cttcttaaca aacacgttac
     7561 taatgaccct cggaaagagt cttcatcggg gcggctccgt caggagtccc acgcttggcg
     7621 cgccggcgga ttgcaacagg gtcatcgcct ttaccacttc gccgtaattc gcctgccggt
     7681 cgccgcggat cagcaccggc gtcttcggcc gcaagcgcag gatcgccgcc actttcctgc
     7741 gcaactcctc ggacgagatg cgccggtcat cgaggaaata ttttccctcg cgatccaccg
     7801 tcacgaccag cgtttcctgg tcctgcgact ccaccggttg cgccgccgcc tgcggcaggt
     7861 ccaccttgac gccctgcgag agcaatggcg ccgtgatcat gaaaatcacc agcagcacca
     7921 gcatcacgtc gatgtacggc acgacgttga tctcgctcat cagcttcttg tggcgcttgc
     7981 ggtagggctc cacgtctagc ccctcgccgc gtacgcctgg cgctgaagta tgctggcaaa
     8041 ctcctgcatg aaaatctcgt aacgcgtcac cagccgctcc accttgttgg agtagcggtt
     8101 gtaggcgatg accgcgggaa tggcggcgaa cagaccgatg gcggtggcga tcagggcctc
     8161 ggcgatgccg ggcgcgacat gcgcgatggt ggcctgctgc accatgccga gggaacggaa
     8221 ggagttcatg atgccccaca cggtgccgaa caggccgaca tacgggctgg tcgaaccgac
     8281 cgtggccagg aatgaaagat gcgtttccag ataatcgatt tcgcgcgaca gcgccacgcg
     8341 catggcgcgc tgcacgccct cgaggatgtc catgggcgcg attctggttt cctgactgag
     8401 acgcttgtat tcgcggaatc cctccacaaa gatgctggcc atgcctgtgg cgttggcttc
     8461 ttttttcgac tgctcggtga acatatggtt gagattggtg ccggaccaga aattggtctc
     8521 gaaccggtcg gccacctggt tcgcgctctt caatgcgaac catttgcgaa agatcatggt
     8581 ccaggaaagc accgaagcgg ccaggaggag cagcatgacc agctgcaccg gcagcgaggc
     8641 gtgccggatg agagacagca atgcaatgcc ttcggttgtg gttatgtcca tgcgctaaat
     8701 tcctgcgtcg atttttgatc cgatgttttc cggaatcggc cgcgggctga acgtggcggc
     8761 atcgagacaa gccactttca cttcgccttc acagagcgcg agctcgtcac gccggatttc
     8821 ctgggcaaag gtaaggctgg ccctgccacg ccggctcacc cgcaccgttg cctgtagcag
     8881 atcgttgaaa cgtgccggct tcaggaattc cagccgcgcg gaacgcacgg caaacaagac
     8941 accctcgcgg cgcgaaagtt catcctgctc gaagcccaag gcgcgcagcc actcggtgcg
     9001 ggcccgttcc ataaatttca gataattcgc gtagtacacc acgttgccgg cgtccgtgtc
     9061 ctcgtaatac acgcggatcg gaatggagaa aatccgttcc atattaattt ttaccgccaa
     9121 gacgccaaga gcgccaggga aagcaaaaca aaaaacgggt cgtttttaaa cctgactctc
     9181 ttggcgtccc tggcgtcctg gcggttcgca aaattattct gcctcaaaca tgccccgctg
     9241 ttcaggtgcg ctcggggcct tgagaccgaa gtgacgccac gccatggcag tggccagacg
     9301 cccgcgcggc gtgcgcatga gatatccctg ctgaatcaga aacggctcga tcacatcctc
     9361 gatggtatca cgctcctcgc cgatggccgc ggcaaggtta tccaccccca ccgggccgcc
     9421 ggaaaatttt tcgatcacag ccagcagcag cttgcggtcg agcatatcaa agccgcactg
     9481 gtccacttct aacatgctga gcgccaattc ggcgatgtcg cccgtcaggc gtccgttgcc
     9541 tttgatctgg gcaaaatcgc gcgcgcgccg caaca
//
LOCUS       DBNGZE010000289         3923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1708053, whole genome shotgun sequence.
ACCESSION   DBNGZE010000289 DBNGZE010000000
VERSION     DBNGZE010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1708053"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1763
                     /gene="polX"
                     /locus_tag="AC16FV_07580"
     CDS             36..1763
                     /gene="polX"
                     /locus_tag="AC16FV_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR2849951.1"
                     /translation="MPVHNADIAAIFDEIADLLEIQGENPFRIRAYRNAAREMQSLGV
                     PASEMIAKGEDLTELPGIGDDLAAKISEIVATGKCKALEKLRTKFPPTITTLLKLPGL
                     GPKRVKMLYDELKVESVAALKQAARAGLIRELPGFGEKTEAAILEALAQHAEEATRFK
                     LAIAAQYAEPLVTYLGKSRNVKQVEIAGSYRRFRETVGDLDILATTTGKSDVMERFVQ
                     YDEVREVMAHGETRATVRLQCGLQVDLRVVPQESFGAALQYFTGSKAHNIEIRRLGQQ
                     KKLKINEYGVFRGKQAIAGATEESVYATVGLPWIAPELRENRGEIEAARKGKLPKLVE
                     LKDLRGDLHAHTRATDGHHTLREMAEAARKRGFEYLAITEPSRRLTVAHGLDVHGLRK
                     QMEEIDRLNAKLSGITLLKGIEVDILEDGSLDLPDEVLGECDLVVGAVHSKFHLSRAK
                     QTARILKAMDHPHFTILAHPNGRLIGSREAYDVDMLKIIRAAHARGCYLELNAHPERL
                     DLLDIHCQMARDEGVLVAISSDAHSIQDFDNLLYGVGQARRGWLEKKDVLNTRPLKLL
                     KPMLGRTIS"
     gene            1894..3717
                     /locus_tag="AC16FV_07585"
     CDS             1894..3717
                     /locus_tag="AC16FV_07585"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR2849952.1"
                     /translation="MTTDPFLIEISRHIWDSKYRFRDGDRVHDQTVDDSWRRIARTLA
                     STEKSDWARWENHFYEALRDFRFLPGGRIQAGAGTGRRVTLFNCFVMGTIEDSMDGIF
                     DGLKEGALTMQQGGGVGYDFSTLRPRGTPAKSVGTVASGPVSFMQIWDAMCGTLLSTG
                     ARRGAMMATLRCDHPDIEAFIDAKRDSHALRRFNLSVLVTDAFMEAMHRDQDWALVFP
                     AEEKIPAGSELVSRTWSGQAQPVRCRVHRRVRARELWDKLMRATYEYAEPGVLFLDRI
                     NRMNNLWYREQINATNPCGEIPLPPYGACDLGSINLTAFVREPFTDRARLDLDEVRKT
                     AAIATRMMDNVIDASLFPLEKQAAQAQGTRHIGLGLTGLADTLIMLGLHYADAAARDI
                     AAQAMQAICHTAYRTSIGLAEEKGPFPFFDREKFLQGKFIQSLPHDIQDGIARLGLRN
                     SHLTAIAPTGTISLLANNVSSGLEPVYEFNNMRRVLEFDGSYTEHKLTDYALRLWRER
                     RGDEKLPAAFVDTHALAPAAHLAMQAALQPYVDNSISKTINVPRDYRFDDFKEIYEQA
                     YDMGLKGCTTFRPNPVTGEVLRGETDSEAGPHCCTVEREAD"
BASE COUNT          808 a         1269 c         1178 g          668 t
ORIGIN      
        1 tctgcgttta tcagcggttg caaaaatcat tcaatatgcc cgtccacaac gccgacatcg
       61 ccgcgatttt cgatgaaatc gccgacctgc tcgaaatcca gggcgagaac ccgttccgga
      121 ttcgcgccta tcgcaacgcc gcgcgcgaga tgcaatccct cggcgttccg gcctccgaga
      181 tgatcgccaa gggcgaggac ctgaccgaat tgccggggat cggtgacgac ctcgcggcga
      241 agatcagcga gatcgtcgct accggcaagt gcaaggcgct cgaaaaactg cgtactaagt
      301 tcccaccgac gatcacgacg ctcctcaagc tcccgggtct gggcccgaaa cgcgtcaaga
      361 tgctttatga cgagctcaag gtcgagtcgg tcgcggcact caaacaggcc gcgcgcgccg
      421 gcctgatccg cgaactgccc ggcttcggag agaaaaccga ggccgcgatc ctcgaagcgc
      481 tcgcgcagca cgcggaagaa gcgacgcgtt tcaaacttgc gatcgcggcg caatatgccg
      541 aaccgctcgt gacctatctc gggaaatcgc gcaacgtaaa acaggttgag atcgccggca
      601 gttaccgccg cttcagggaa accgtgggcg atctcgacat cttggccacc acgacgggca
      661 agtccgatgt catggagcgc ttcgtgcagt atgacgaagt gcgcgaggtg atggcccacg
      721 gcgagacgcg tgccacggtg cggctccagt gcgggctgca ggtggacctg cgcgtcgtgc
      781 cgcaggagtc gttcggcgcg gcgctccagt acttcaccgg atcgaaggcg cacaacatcg
      841 aaatccgccg actgggacag cagaaaaaac tcaaaatcaa cgaatacggc gtgtttcgcg
      901 gcaagcaggc cattgcaggc gcgacagaag agtcggtcta tgccacggtg gggctccctt
      961 ggatcgctcc ggagctgcgc gaaaaccgcg gcgagatcga ggccgcgcgc aagggaaaac
     1021 tgcccaagct cgtggaactc aaggatttgc gcggcgatct gcacgcccac accagggcca
     1081 ccgacggtca tcatacgctc agggagatgg ccgaggcggc gcgcaagcgc ggcttcgaat
     1141 atctcgccat caccgaaccc tcgcgccggc tcaccgtcgc gcacgggctc gatgttcacg
     1201 gactgcgcaa gcagatggaa gaaatagacc ggctgaacgc gaaactctcc ggcatcacac
     1261 tcctgaaagg catcgaggtg gacattctcg aagacggcag cctcgacctg ccggacgagg
     1321 tgctcggcga gtgcgatctg gtggtgggcg cggtgcacag caaattccat ctgtcacgcg
     1381 ccaaacagac cgcgcgcatc ctcaaggcca tggaccaccc gcacttcaca atcctcgccc
     1441 atcccaatgg acgcctcatc ggaagccgcg aggcctatga cgtggacatg ctcaagatca
     1501 tccgcgcggc gcacgcacgc ggctgttatc tcgaactaaa cgcccacccg gagcggctcg
     1561 acctgctcga catccactgt cagatggcgc gtgacgaagg cgtgctcgtg gccatcagct
     1621 ccgatgccca cagcatccag gattttgaca atctcctgta cggtgtgggc caggcccgac
     1681 gtggctggct ggaaaagaag gacgtgctca ataccagacc gctcaagctg ctcaaaccga
     1741 tgcttgggcg tacaatttcc tgaacagagg aattggagcg gacacaaaag ccgtgccgct
     1801 caattccgcc aggcgaagtg aaaaggagaa agcaagtatc tggcgtttcg cgatcagtac
     1861 tttccacttt acactgtgct ttttaccaat cctatgacca ccgacccttt ccttatcgag
     1921 atttcccgcc atatctggga cagcaagtac cgcttccgcg acggcgaccg ggtacacgac
     1981 cagaccgtgg acgacagctg gcgacgcatc gcacgtacgc tggccagcac ggaaaaatcc
     2041 gactgggccc ggtgggaaaa tcatttttat gaagcgctgc gggattttcg attcctgccc
     2101 ggcgggcgca tccaggccgg ggccggcacc ggccggcgcg tgacgctgtt caactgcttc
     2161 gtcatgggca cgatcgagga ttcgatggac ggcatcttcg acggcctcaa ggaaggcgcg
     2221 ctcaccatgc aacagggcgg cggcgtgggt tacgacttct ccacgctgcg gccccggggc
     2281 acgccggcga aaagcgtcgg cactgtcgcc tccggcccag tgtcgttcat gcagatctgg
     2341 gatgccatgt gcgggacgct gctttccacg ggcgcgcggc gcggggccat gatggcgacc
     2401 ctgcgttgcg atcatcccga tatcgaggcc ttcattgacg ccaagcgcga cagccacgcg
     2461 ctgcgacgct tcaatctctc ggtgctggtg acggatgcct tcatggaggc catgcaccgg
     2521 gaccaggact gggcgctggt tttcccggcg gaagagaaaa tccccgcggg aagcgaattg
     2581 gtttcgcgga cctggtcggg ccaggcccaa ccggtgcgct gccgcgtcca ccggcgcgta
     2641 cgcgcccgcg agctgtggga caagctcatg cgcgcgactt acgaatacgc cgagcccggc
     2701 gtgctgttcc tcgatcgcat caatcgcatg aacaacctct ggtatcgcga gcagatcaac
     2761 gccacgaatc cctgtggaga aatcccgttg ccgccatacg gcgcctgcga tctcggttcc
     2821 atcaatctga cggcctttgt acgcgaaccc ttcacggatc gcgcgcgcct cgatctggat
     2881 gaggtccgca agaccgcggc cattgccacg cgcatgatgg acaatgtcat cgacgcctcg
     2941 ctgttcccgc tggaaaaaca ggcggcacag gcgcagggca cgcggcatat cgggctcggt
     3001 ctcacgggac tggcggatac gctgatcatg ctcggactgc actatgccga tgcggcggcg
     3061 cgcgatatcg cggcacaggc catgcaggcc atctgccata ccgcctaccg cacttccatc
     3121 ggactggcag aggagaaagg tccatttccg ttcttcgacc gggaaaaatt cctgcaggga
     3181 aaattcatcc agagcctgcc gcacgatatc caggatggca tcgcccgctt aggattgcgc
     3241 aacagccacc tgacggcaat cgcgccgact ggcaccatca gcctgcttgc gaacaatgta
     3301 tccagcgggc tcgagccggt gtacgagttc aataatatgc gccgtgtgct ggaattcgat
     3361 ggcagctata ccgaacacaa gctcaccgat tacgcgttgc gcctgtggcg tgaacggcgc
     3421 ggtgatgaaa aactgccggc ggcgttcgtg gacacccacg cgctggcgcc cgcggcgcat
     3481 ctcgccatgc aggcggcgct ccagccctac gtcgacaact ccatctccaa gaccatcaac
     3541 gtcccgcgcg actaccgctt tgacgacttc aaagagattt acgaacaggc ctacgacatg
     3601 ggtctcaagg gctgcaccac cttccggcct aatcccgtca ccggcgaggt gctgcgggga
     3661 gaaaccgaca gcgaggccgg gccgcactgt tgcaccgtcg agcgcgaggc ggactgaaag
     3721 ctgcctcaaa acctgctgcg cactgcgctc ctttcccgct gcgccatccg gctttgcggg
     3781 aaagtccggg gccgccatcc ctggcggccc gttcggcggc acgaacttcg cgcttcgcca
     3841 tccctgcttc gcgcgaagtc cagcccgccc atccctgggc gggcgttcgc ggcttgcgcc
     3901 gctcaccccc cggacgttcg tga
//
LOCUS       DBNGZE010000290         5325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_110791, whole genome shotgun sequence.
ACCESSION   DBNGZE010000290 DBNGZE010000000
VERSION     DBNGZE010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_110791"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC16FV_07590"
     CDS             <1..425
                     /locus_tag="AC16FV_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR2849953.1"
                     /translation="VVVDYAHTPDALEKILLALREHTRGKLWCVFGCGGDRDRGKRPV
                     MGEIAERLADGVLLTDDNPRHESGDAIIADIAGGMHAAPRIIRDRRVAIATAIEGAQA
                     DDIVLIAGKGHEDYQQVGDERRPYSDREVVKQLMEAAA"
     gene            425..1792
                     /locus_tag="AC16FV_07595"
     CDS             425..1792
                     /locus_tag="AC16FV_07595"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR2849954.1"
                     /translation="MSLSILADVLKCPHKGPDAVFTGVCHDTRNLQPGDLYVAIKGDR
                     FDGHNFLAEAISAGAVGALLARDMETPLPYVRVPNTRLALGNLGAFWRNQFQIPVIAV
                     TGSNGKTTVKEMIGAIMGEGGPGCVTRGNLNNDIGVPLTLTRMRASDRYAVIEMGMNH
                     FGEIEYLSRLTRPTVALITNAGEAHLAGVGSVEGVARAKGEIFTGLRTGGTAVLNADD
                     EFFPLWKTLAATRKCLTFGLDHQADVSAEYRFTDNGSLVHLKTTTHGDIDMRVPLLGR
                     HNVMNTLAGAGAAIAAGASLDQVKSGLEKLKTVSGRLEVKRGISGARIIDDTYNANPA
                     SVAAGLQVLKEFKGERVLVLGDMGELGPSAADIHFRVGELASRIGIQRLFAIGELSRI
                     AVGAFGKGARHFESPEGLIEALQDCLHADMTVLVKGSRMMRMERIVAGITKPSPSVTG
                     NGGSR"
     gene            1793..2875
                     /gene="mraY"
                     /locus_tag="AC16FV_07600"
     CDS             1793..2875
                     /gene="mraY"
                     /locus_tag="AC16FV_07600"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR2849955.1"
                     /translation="MLYYLFESLARDFTFFNVFHYLTLRGILGVLTALLISFWIGPTM
                     IRLLGVRQLGQTVRKDGPQSHLAKAGTPTMGGAMILVAIAISTLLWADLSNRFVLAVL
                     FATASFGAIGWVDDYKKIINKDPRGIGAKAKFFWQTASGLAIALFLYYTAKSPADTAL
                     ILPLFKNIIIDLGPVYILLAYLVIVGSSNAVNLTDGLDGLAIMPTVMVAAALGIFAYV
                     MGHLKFSAYLGFPYIAGAGEVLIFCGAIVGAGLGFLWFNTYPAMVFMGDIGALALGAA
                     LGTVAVSVRQEIVLFIMGGVFVMETVSVMLQVASFKLTGKRIFRMAPLHHHFELKGWP
                     EPRVIVRFWIISLILVLIGLATLKIR"
     gene            2895..4235
                     /gene="murD"
                     /locus_tag="AC16FV_07605"
     CDS             2895..4235
                     /gene="murD"
                     /locus_tag="AC16FV_07605"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR2849956.1"
                     /translation="MTKMETKRALVVGLGLTGISCVRHLVARGYDVAVVDTRAQPPKL
                     AELQREFPRVAVHTGGLPAALFNNPGLLVVSPGVSVKEAEIARAVEQGAEAIGDIELF
                     AREATAPVIAVTGANGKSTVTALAGELCRQAGLKTAVGGNIGVPALSLLADSAPDVYV
                     LELSSFQLETTASLNARAATVLNITPDHMDRYRDIGEYAEAKARVFRGNGTMVLNTDD
                     PLVMRMRQSGRRVVAFGLGRPLAAQDYGLDEIRGETWLVHGKQPLMPAREVPLAGRHN
                     LANVLAAMALAEAVGVMPEAACVAVRAFKGLEHRTQLVTEHRGVRWYDDSKGTNVGAT
                     VAALNGMTTPVVLIAGGDGKGQDFSDLKPACVQHARAVVLIGRDAPQIEAALGKAVPV
                     QHAKDMHDAVRIAKGLAQAGDVVLLSPACASFDMFRNYEHRAEVFRAAIQETLK"
     gene            4232..>5325
                     /gene="ftsW"
                     /locus_tag="AC16FV_07610"
     CDS             4232..>5325
                     /gene="ftsW"
                     /locus_tag="AC16FV_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR2849957.1"
                     /translation="MMAAVMTGEVRAAGRARLAWDPWLLGSSFVLLATGVIMVYSASV
                     AYADREMGNSAYFLIRHASSVILGLVLMAFTMRTRVRWWEKAGPYLLLIGVVALSVVL
                     LPGVGAHVNGSSRWIRLGIVNLQPSEFMKLFAVVYVAGYLVRKQEALKNFTQGIVMVS
                     LVVAVIGALLLMEPDMGSLVVITVTVFAMLFLGGVRFWHFMMVLGVGVGSMVLLTIVS
                     PYRMARATSFMDPFADPFNTGFQLVQALIAFGRGEWFGVGLGASVQKLFYLPAAHTDF
                     VFAVMAEELGLVAVLGMMALFGVILVRAFSIARQAEAAGQIYGARLAQGIGLLLGGQG
                     MINMGVNMGLLPTKGLTLPFVSYGGSSMLV"
BASE COUNT          900 a         1616 c         1780 g         1029 t
ORIGIN      
        1 tggtggtggt ggattacgcg cacacgccgg acgcgctgga aaaaatcctg ctcgcgttgc
       61 gcgaacacac gcgcggaaaa ctctggtgtg ttttcggctg cggcggcgat cgcgaccgtg
      121 gcaagcgccc ggtgatgggc gagattgccg agcgcttggc ggacggggtg ttgctgaccg
      181 acgacaatcc gcgccacgag tcaggcgatg ccatcatcgc tgacatcgcc ggtggcatgc
      241 acgccgcgcc tcgcatcatc cgcgatcgcc gggtggcgat cgccacagcg atcgaaggcg
      301 cgcaagcgga cgacatcgtg ctcatcgccg gcaagggtca cgaggattac cagcaggtgg
      361 gcgatgaacg tcggccttac agcgaccgcg aagtagtaaa acagctgatg gaggcggccg
      421 cgtgatgtct ctgtcgatac tcgccgacgt cctcaagtgc ccgcacaagg gaccggacgc
      481 cgttttcacg ggcgtgtgtc acgacacacg caacctgcag ccgggcgatt tgtatgtcgc
      541 gatcaagggc gatcgcttcg acggacacaa ctttctggcc gaggccatca gcgccggcgc
      601 ggtcggcgcg ttgctggcgc gcgacatgga aacaccgctg ccctatgtgc gcgtgccgaa
      661 cactcgcctg gcgcttggta acctcggcgc attctggcgc aaccagtttc agattccggt
      721 catcgccgtc actggcagca acggcaagac cacagtcaag gaaatgatcg gcgccattat
      781 gggcgagggc ggccccggct gcgtcacgcg tggaaacctc aacaacgaca tcggtgtgcc
      841 gctgacgctc acgcggatgc gcgccagcga ccgctacgcc gtgatcgaaa tgggcatgaa
      901 ccatttcggc gagatcgaat atctctcgcg cctgacgcgc ccgacggtgg cgctcatcac
      961 caacgccggc gaggcgcatc tggcgggcgt cggctcggtc gaaggtgtgg cgcgcgccaa
     1021 gggcgaaatt ttcaccggtc tgagaacagg cggcacggcc gtgttgaacg ccgatgacga
     1081 attttttcct ctctggaaaa cgctggcagc gacgcgcaaa tgcctgacct tcgggctgga
     1141 tcaccaggcc gacgtgagcg cggaataccg gtttaccgac aatggcagtc tcgttcatct
     1201 gaaaaccact acccatggag acatcgacat gcgcgtaccc ctgttgggca gacacaatgt
     1261 catgaatacc ctggcgggcg cgggcgcggc gatcgcggcc ggcgcctcgc tcgatcaggt
     1321 caaatccggg ctcgaaaaac tcaagacggt ttccggccgc ttggaagtga agcgcggcat
     1381 cagcggcgcg cgcatcatcg acgacaccta taacgccaac cccgcctcgg tggcggcggg
     1441 gctgcaggtg ctcaaggaat tcaaaggcga gcgcgtgctg gtgctgggcg acatgggcga
     1501 gctcggaccg tccgccgccg acatccattt ccgtgtgggc gaactcgcca gccgcatcgg
     1561 cattcagcgc ttgttcgcca tcggtgagct ttcgcgcatc gccgtgggcg ccttcggcaa
     1621 gggtgcacgg catttcgaga gccccgaagg cctgatcgag gcgttgcagg actgcctgca
     1681 tgcggacatg acggtattgg taaagggttc gcgcatgatg cgcatggagc ggattgtcgc
     1741 cggcatcacg aagccatctc catctgtcac cggaaacggg gggagccgtt aagtgctcta
     1801 ttatctcttc gaatcgctgg cgcgggactt cacgtttttc aacgtgtttc attacctcac
     1861 gctgcgcgga atcctgggcg tgttgacggc gctcctgatc agtttctgga tcgggccgac
     1921 gatgatccgg ctcctcggcg tgcgccagct ggggcagacc gtgcgcaagg acggtcccca
     1981 gagtcatctc gccaaggccg gcaccccgac catgggcgga gcgatgattc ttgtcgccat
     2041 cgctatctcc accctgttgt gggcggatct gtccaaccgt ttcgtgctgg cggtgctgtt
     2101 cgccaccgcc tccttcggcg cgatcggctg ggtcgacgac tacaagaaga tcatcaacaa
     2161 ggacccgcgc ggcatcggtg ccaaggccaa gtttttctgg cagacggcat ccggtctggc
     2221 gattgctcta ttcctttatt acaccgccaa gagcccagcc gataccgcgc tcattttgcc
     2281 gctgttcaag aacatcatca tcgatttggg cccggtttat attctgctcg cctatctggt
     2341 gatcgtgggc agcagcaacg ccgtgaacct gacggacgga ctcgatgggc tggccatcat
     2401 gccgacagtc atggtggccg cggcgctggg catcttcgcc tacgtcatgg gtcacctcaa
     2461 gttttccgcc tacctcggtt ttccctacat cgccggcgcg ggcgaggtat tgatcttctg
     2521 cggcgccatc gtcggcgccg gactcggatt cttgtggttc aacacctatc ccgccatggt
     2581 gttcatgggc gatatcggtg cgttggcgct gggcgccgcg ctcggcacgg tggcggtgtc
     2641 ggtgcgccag gaaatcgtgc tgttcatcat gggcggggtg ttcgtgatgg agacggtgtc
     2701 ggtgatgttg caggtggcgt cgttcaaact caccggcaag cgcatcttcc gcatggcgcc
     2761 gctgcatcat cattttgaac tgaagggttg gcccgaaccg cgcgtgatcg tgcgcttctg
     2821 gatcatatcg ctgattctgg tgttgatcgg attggcaaca ttgaaaattc gctagcaacg
     2881 tgaaggccgt cgcgatgaca aagatggaga caaaacgcgc actggtggtg ggtctcgggc
     2941 tcaccgggat ttcctgcgtg cgccatctgg tcgcgcgcgg ttatgacgtc gcggtagtgg
     3001 acacgcgcgc gcaaccgccg aagctggcgg aactgcaacg cgaattcccg cgggtcgcgg
     3061 tgcacaccgg cggtttgccc gcggcgcttt tcaataaccc cggcctgctg gtggtaagcc
     3121 ctggtgtatc ggtcaaggag gccgagattg cgcgcgcggt ggaacagggc gcggaggcca
     3181 tcggcgacat cgagctgttc gcgcgcgagg cgactgcccc ggtcatcgcc gtgaccggcg
     3241 cgaacggcaa aagcacggtg acggcgctgg ccggagagct gtgccggcag gcgggtctca
     3301 agactgcggt cggcggcaac atcggcgtgc cggcgctgtc gttgctcgcc gattccgcac
     3361 cggatgtcta cgtgctcgag ctgtcgagtt tccagctgga aacgaccgcc agcctcaacg
     3421 cgcgcgctgc cacggtgctc aacatcacgc cggatcacat ggaccgttac cgcgacatcg
     3481 gcgaatacgc cgaggccaag gcgcgcgtct tccgcggcaa cggtaccatg gtgctcaaca
     3541 ccgacgatcc tctggtgatg cgcatgcgcc agtcgggccg gcgcgtggtc gctttcgggc
     3601 tcggccgccc gctggcggcg caggattacg gcctggatga aattcgcggc gagacctggc
     3661 tcgtgcacgg caagcagccg ctcatgcccg cgcgcgaggt accgctggct ggccggcata
     3721 acctggcgaa cgtactcgcc gccatggcgc tggccgaggc tgtcggtgtc atgcccgagg
     3781 cggcctgcgt cgccgtgcgc gcattcaagg gactcgagca tcgcacccag ctggtaaccg
     3841 agcaccgggg cgtgcgctgg tatgacgatt ccaaggggac caatgtcggc gccacggtgg
     3901 cggcgttgaa cggcatgacc acaccggtgg ttctcatcgc cggcggcgat ggcaagggcc
     3961 aggatttcag cgatctgaaa ccggcgtgcg tccagcatgc ccgcgcggtg gtgctcatcg
     4021 ggcgcgatgc gccgcagatc gaggccgcgc tcggtaaggc cgtgccggtg cagcatgcga
     4081 aggacatgca cgatgccgtg cgcatcgcca agggattggc gcaggcggga gacgtggtgc
     4141 tgctgtcgcc ggcctgcgcc agcttcgata tgttccgcaa ttacgaacac cgcgccgaag
     4201 ttttccgtgc agcaattcag gagacattga aatgatggcc gcggtcatga cgggtgaagt
     4261 gcgcgcggcc ggccgggcac gactggcttg ggacccgtgg ctgctgggca gcagcttcgt
     4321 gctgctcgcg accggcgtga tcatggtgta ctcggcgtcg gtggcctacg ccgaccggga
     4381 aatgggcaac agcgcctatt tcctgatccg ccacgcctcc tccgtaattc taggtctggt
     4441 gcttatggcg ttcaccatgc gcacgcgcgt gcgctggtgg gaaaaggccg ggccgtatct
     4501 gctgctgatc ggcgtcgtcg cattgtccgt ggtgctgctg ccgggcgtcg gcgcgcacgt
     4561 caacggcagc tcgcgctgga tccggctcgg tatcgtgaac ctgcaaccgt cggaattcat
     4621 gaagctcttc gcggtggtgt atgtcgccgg ctatctggtg cgcaagcagg aggcgctcaa
     4681 gaatttcacc cagggcatcg tgatggtcag tctggtggtg gcggtgatcg gcgcactgct
     4741 gctgatggaa cccgacatgg gttcgctggt ggtcatcacc gtgacggtgt tcgccatgct
     4801 gttcctgggc ggcgtgcgct tctggcattt catgatggtg ctcggcgtcg gcgtcggcag
     4861 catggtgctg ctcacgatcg tctcgccgta tcgcatggca cgcgctacca gtttcatgga
     4921 cccgtttgcg gatccgttca acaccggatt ccagctggtg caggcgctga tcgccttcgg
     4981 acgcggcgag tggttcggcg tgggtctggg cgcgagcgtg cagaaacttt tttatttacc
     5041 cgcggcgcat accgatttcg tgttcgccgt gatggccgag gaactggggc tggtggcggt
     5101 gctcggcatg atggcgctgt tcggcgtcat cctcgtgcgc gccttctcca tcgcgcgcca
     5161 ggcggaagcc gccggccaga tttacggcgc gcgtctggcg cagggcatcg gcttgctgct
     5221 cggcggtcag ggcatgatca acatgggcgt gaacatgggc ctgctgccga ccaaaggcct
     5281 cacgctcccg ttcgtgagct acggcggttc cagcatgctg gtgag
//
LOCUS       DBNGZE010000291         1778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_457759, whole genome shotgun sequence.
ACCESSION   DBNGZE010000291 DBNGZE010000000
VERSION     DBNGZE010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_457759"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16FV_07615"
     CDS             <1..272
                     /locus_tag="AC16FV_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HIR2849958.1"
                     /translation="EMVESRLAWLRSWQRPLLRPLLAKLLRQFIDVFYYPDARLYELA
                     GLRPGAEWVARAQANAHRRQFMERCLHPTLEIFRQRGFPLAETVK"
     gene            complement(300..1259)
                     /locus_tag="AC16FV_07620"
     CDS             complement(300..1259)
                     /locus_tag="AC16FV_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR2849959.1"
                     /translation="MDKTILKTLVPINSLTPENFRELETRAIIENLSAGSQLFKQGDR
                     DNHSIFLLSGEVALSSSDAAVNRNVIAGTDEARYALAQLKPRQYTGVAKTPVTIARVD
                     SALLDRLLTWDQTTGYEVTEFDSSQDPEWMIRMLRSETFQRLPPSNINALFARFEPVE
                     VKTNQVVIRQGDPGDFYYIVKSGKASVSRKADKTGKVSVVGQLGEGDGFGEEALLSGA
                     PRNATVVMTADGVLMRLVRMDFDALMREPLVKWVTLDQARSMAQSGAGLLDVRTEDEF
                     RHGTVKGSLNLPLYLLRLKASVLDAKRHYVVFCQTGSRSCAAV"
     gene            complement(1329..1417)
                     /locus_tag="AC16FV_07625"
     tRNA            complement(1329..1417)
                     /locus_tag="AC16FV_07625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1380..1382),aa:Ser,seq:cga)
BASE COUNT          362 a          524 c          496 g          396 t
ORIGIN      
        1 gcgagatggt ggaaagccgt ctggcctggt tgagaagctg gcagcgcccg ctgttgcggc
       61 ctttactcgc aaagttgctg cgtcagttta ttgacgtttt ctattacccg gacgcacggc
      121 tctatgaact tgcgggtctt cgtcccggtg cggaatgggt ggcgcgcgca caggccaatg
      181 cgcatcgccg ccaattcatg gagcgctgcc tgcatcccac gctggaaatc tttcgtcagc
      241 ggggattccc gcttgcagaa acggtgaagt agaaaatcag gcaggatgtg cgatggcgtt
      301 cagaccgccg cgcaactgcg gctgccggtc tggcaaaaaa caacatagtg acgtttggca
      361 tctagcacgg aggccttgag ccgcaacaga tacagcggca gattcaaact gcccttgacg
      421 gtaccgtggc ggaattcgtc ttccgtccgc acatcgagca accccgctcc tgattgcgcc
      481 atggacctgg cctgatccag cgtcacccac ttcaccagcg gctcccgcat cagcgcatcg
      541 aaatccatgc gcaccaggcg catgagcaca ccatccgccg tcatcaccac cgtggcattg
      601 cgcggcgcgc cggatagcag cgcctcttcg ccgaagccgt cgccttcacc cagttgtccg
      661 acaacggaaa ccttgccggt tttatctgcc ttgcgcgata cgctggcctt gccggatttg
      721 acgatgtaat aaaaatcgcc cggatcgccc tgccggatga cgacctgatt tgtcttgacc
      781 tcgaccggct cgaagcgggc aaataacgcg ttgatattgg aaggcggtaa cctctggaat
      841 gtttcgctgc gcagcatgcg gatcatccat tccgggtcct ggctgctgtc gaattcggtt
      901 acttcataac cggtcgtttg atcccaggta agcagacgat ccagcagagc actgtccacc
      961 cgcgcgatag tcacgggtgt cttggcgacg ccggtgtact ggcgcggttt gagttgcgcc
     1021 agcgcatagc gtgcctcgtc ggtgccggca atgacattac gattgacagc ggcatcggag
     1081 gaagagagcg ccacttcacc tgacaacaga aatatcgaat gattatcacg gtcgccctgc
     1141 ttgaacagct ggcttcctgc cgacagattc tcgatgatgg ccctggtttc gagctccctg
     1201 aaattctcag gcgtcagcga attaatcggg accagggttt tgaggatggt tttgtccatt
     1261 ccgtggcatg actgtgtaaa ttaataaaca gtctagacca gattacctag gaacattgga
     1321 cggaaaaatg gcggagaggg agggattcga acccccgtgg cgtttccgcc catctgattt
     1381 cgagtcagcg ccgttgtgac cgcttcggta cctctccggg gaaagaaatt atatcaacaa
     1441 tcgtgtcttg agcttaattt aatagcggga gggattactc ggcgcgtccc tgcgcctcgc
     1501 cccttcgggg ccgccgctgt tgcgtcctta gctccgtaat cgatgcatcc ctgcaccact
     1561 cctgcggcgt tcgggatcgc tcccggcgat cctcgtcgaa cccccatggc gtttccgccc
     1621 atctgatttc gagtcagcgc cgttgtgacc gcttcggtac ctctccggag gggcgggatt
     1681 ataccagcac acaaggtttc agccacgcca aagcatgcgt gacaagggct ttccactgca
     1741 ctagttttac ttctatgtat cgcaagcagc ccggacaa
//
LOCUS       DBNGZE010000292         1542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1152868, whole genome shotgun sequence.
ACCESSION   DBNGZE010000292 DBNGZE010000000
VERSION     DBNGZE010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1152868"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..>1542
                     /locus_tag="AC16FV_07630"
     CDS             199..>1542
                     /locus_tag="AC16FV_07630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR2849960.1"
                     /translation="MQVAKHDRRRADYSLKKGGAARAQGRETHESFGIDVNAGTSINT
                     WGIDSMSDDIPIVIPAFLVSTGIVFYTGIQAIVVGSLGRRVPLYLTFAAVCLCAAGFL
                     GTTTLYYTAASVATAAAALRWQGAFILLLLPAFFVYVGLYTGQARIKPWLAALALVCA
                     GLLILNFTSPYGLRFSTLEATAPLSLPWGEQLANYSGTFSPWNGFMRVLVFVIFGWAI
                     WRTAILFQRGQRRRAAFFAAYLVLQFAATIWGALIDMGLVHSFYVTGFAFLGLVLLMS
                     VCMGIDLRDQTANLEMLTAKLRMEIDERRKAETQIRWMANRDDLTGLANRASLHQHLT
                     EVLDQATRSEQYNAMLLIDLDHFKTINDALGHDVGDEALREVARRLTNTVADYASLDI
                     FLVRLGGDEFVIVISGISRDKEEATTVSRELAERVIENMLRPLVVGERILNVGASI"
BASE COUNT          277 a          482 c          455 g          328 t
ORIGIN      
        1 acgccggctg acatcgcgcc gccggattca gcagcttcgc cgcccggccc aggacgcgga
       61 ccttcccaac cggaaacgcg tcctgaacgc ctttcgcagc cgaagcatga ctcagccgcc
      121 cagccgcggc tcgcatgcca gcaccgggaa acaccagtca tttcaaggat attccggcag
      181 cacaggtatt gccggcgctt gcaggttgcc aagcatgaca gacggcgcgc agactatagt
      241 ttaaaaaaag ggggcgccgc ccgtgcgcaa ggtcgggaaa cccatgaatc tttcggcata
      301 gacgtcaacg cagggacatc gatcaacact tgggggattg attctatgtc tgacgacata
      361 ccgatcgtca tccccgcatt cctcgtcagc acggggattg tgttttatac cggcatccag
      421 gccatcgtcg ttgggtcgct gggccggcgc gtgccgcttt atctgacctt cgcggcggtt
      481 tgcttatgcg ccgccggttt cctgggcacc accaccctgt attacacggc tgcttcggtg
      541 gccacggccg ccgcggcgct gcgctggcag ggcgctttca tcctgctcct tctgccggca
      601 ttttttgttt atgtcgggct ttataccggt caagctcgaa tcaaaccatg gttggcggcg
      661 ctcgccctcg tctgtgccgg cctgctgatc ctcaatttca cgtcgcccta cggtctccgc
      721 ttctcgacgc tggaggcgac ggcgccgttg tccctgccgt ggggagagca gctcgccaac
      781 tactccggaa cgttctcgcc ctggaacgga ttcatgcgtg ttctggtttt cgtgattttc
      841 ggctgggcca tctggcgcac cgcgatactg ttccaacgcg gtcagcgacg ccgcgcggct
      901 tttttcgccg cctacctggt gctgcaattt gcggccacca tctggggggc gttgatcgac
      961 atgggtctgg ttcactcctt ctatgtgacc ggcttcgcgt ttctcggact ggtgctgctc
     1021 atgagcgtgt gcatgggcat cgacctgcgc gaccagaccg ccaatctcga gatgcttacc
     1081 gccaagctcc gaatggaaat cgacgaacgc cgcaaggccg agacgcaaat ccgctggatg
     1141 gcgaaccggg acgatttgac cgggctggcc aaccgggcgt cgctgcacca gcatctgacg
     1201 gaggtgctcg accaggcaac gcggtctgaa caatataacg cgatgctctt gatcgatctg
     1261 gatcacttca aaacgatcaa cgacgcgctc ggccacgacg tgggcgatga ggcgttgcgc
     1321 gaagtcgccc gccgcctgac gaacaccgtc gccgactacg cctccctcga tatctttctc
     1381 gtgcgtctgg gcggcgatga gtttgtgatc gtcatatccg ggatatcccg ggacaaggaa
     1441 gaggcgacga cggtatcccg cgagctagcg gaacgggtaa ttgaaaatat gttgcgtccg
     1501 cttgtcgtgg gtgaacgcat cctcaatgtc ggcgccagca ta
//
LOCUS       DBNGZE010000293         1537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_111321, whole genome shotgun sequence.
ACCESSION   DBNGZE010000293 DBNGZE010000000
VERSION     DBNGZE010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_111321"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..>1537
                     /gene="ppx"
                     /locus_tag="AC16FV_07635"
     CDS             202..>1537
                     /gene="ppx"
                     /locus_tag="AC16FV_07635"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HIR2849961.1"
                     /translation="MKGHKRSPKIVTSAKGRLDHGPPIAVIDIGSNSIRLVIYEGLTR
                     SPTPIFNEKVLAGLGRQVQSTGLLPADAIDTALDALRRFRALCDILHVHRVWAIATAA
                     CRDARNGRAFIAEAERICRTTITILSGKREAHLTALGIVSGIHKPDGIVGDLGGGSLE
                     LVDVHGSRVRGGVTLPLGGLALQDITGKSLRKAEKFVEEAFEDLDMLHGGEDRTFYAV
                     GGTWRALARLHMWQTGYPFHVMHNYRISAREAQEFSRLVHHVDTETLSKIEVVNAARR
                     PLLAYAALVMENLVRTIKPRQVIISVLGVREGLLYSLLNQRERARDPLISAAADLNVL
                     RSRSPRHGEELIAWTDRLMTSSGLEESSDERRLRHAACLLGDIGWRAHPDYRGEQSMN
                     IIAHGAFVAIDHPGRAYLALSVYFRHAGLSEEELSPRLRELATTRMLDRARVL"
BASE COUNT          291 a          491 c          447 g          308 t
ORIGIN      
        1 acacccgcac ccttagagag caaggggtgc ggcaccccaa aacatctcac gtgcgcgaat
       61 ggtggtccac ccgtccggga tgcctgagcc acgcgccttg cgccaccggc ccctcgcctc
      121 cccttagtcg ctaaagacct ggtcgaaccg ctgcttgaaa cacgctccca aagtagcgtc
      181 ctcaccacct ctcagcacat catgaagggg cataagcgtt cgcccaaaat cgtcacgtcg
      241 gcgaaaggcc gtctcgatca tggccctccg atcgccgtca tcgatatcgg ctcaaactca
      301 atccgtcttg ttatctatga aggtctgacg cgctcaccga caccaatttt taatgagaaa
      361 gtgctggccg gcctcgggcg gcaagtgcag tccacaggac tattgcccgc tgacgccatc
      421 gacacagcgc tcgatgcgct ccggcgtttt cgcgccttgt gcgatattct gcatgtccac
      481 cgtgtttggg cgatcgctac ggccgcttgc cgcgatgcac gcaacggccg cgcattcatc
      541 gccgaagccg aacgcatctg ccgcaccacg atcacaatcc tttccggaaa gcgcgaggct
      601 catctcactg cgctcggcat cgtttccggc atccataagc ccgatggtat tgtcggagat
      661 ctgggcggcg gctcgcttga gctggtcgac gtgcacggct cccgtgtgcg cggtggcgtg
      721 acgctgccgc tcggcgggct tgcgctccag gacatcaccg gaaagtcgct gaggaaagcg
      781 gagaaattcg tcgaggaagc cttcgaagac ctcgatatgt tgcacggcgg cgaggaccga
      841 acgttttacg cggttggcgg cacttggcgc gcgctggcgc gactgcacat gtggcaaacc
      901 ggctatccat ttcacgtcat gcacaactac cgcatctcgg cgcgggaggc gcaggagttc
      961 tccaggctcg tgcatcacgt cgataccgag acgctgtcga agatcgaagt cgtaaacgcg
     1021 gctcgccgac ctctgctcgc ctatgccgcg ctggtgatgg agaacctggt ccgtacgatc
     1081 aagcccagac aggtcatcat ttctgtgctc ggtgtgcgcg aaggcctgct gtattcactg
     1141 ctcaatcaac gtgaacgcgc gcgagatccg cttatctcgg cggcggccga tctcaatgtg
     1201 ctgcgctccc gctcgccgcg ccacggcgag gagttgatcg cgtggaccga ccgcttaatg
     1261 acgtcatcag gactagagga aagctccgat gagcgccggc tgcgccatgc cgcttgtctg
     1321 ttgggagaca ttggctggcg cgcacatccg gattatcgcg gcgagcagtc aatgaacatc
     1381 atcgcgcatg gtgctttcgt tgctattgat catccgggcc gcgcgtacct ggcattgtca
     1441 gtctatttcc gccacgccgg tctgagcgaa gaggaattgt cgcctcggct gcgcgaactt
     1501 gccacaacac gcatgctgga tcgcgcacgt gtgctcg
//
LOCUS       DBNGZE010000294         3800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1079299, whole genome shotgun sequence.
ACCESSION   DBNGZE010000294 DBNGZE010000000
VERSION     DBNGZE010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1079299"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16FV_07640"
     CDS             complement(<1..461)
                     /locus_tag="AC16FV_07640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2849962.1"
                     /translation="MSAGRIIKHGLAAVFPSLVLIQQRRAKRDIAITFDDGPDPRHTP
                     RILDILDAAGASATFFLQGKIAEQHPALVRKVAARGHQIGNHGYSHLDARRSNLRAYV
                     EETMLAQNVLENILGRRIDKIFRPPYGNITGPSFLALARRGFRFIFWSVDSR"
     gene            complement(461..1810)
                     /locus_tag="AC16FV_07645"
     CDS             complement(461..1810)
                     /locus_tag="AC16FV_07645"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR2849963.1"
                     /translation="MNLYQDVFFRSLDFLRGRRTIERLHFLRRSQHWDRATLRSWQLA
                     RLNELLRQARDNSDYYGKLLRDVPLPLKGLEDLAHIPVLTKTLIQENFNGLQCRNMPR
                     SRFVLARTGGSTGEPTYYFWDKRGMDWNRASVYRSAEWAGVALGERTVQMSGSHFDYN
                     NAQKLLHRLTYFLQRYRDYSVAFLTEDLLERYYQGICRWRPTSIWGYASGIHAFADYI
                     RKRHPGTSFDFLRALITSSETLRPEQRGLINQVFGDNKVHDHYGSREMYMGAECHEHR
                     GYHLHADVLLVETVDRENRPCRPGELGRILITDLSNLVFPFIRYEIGDVGVMGDDAPC
                     PCGVTLPRLARIEGRIADILVLPDRLLTTPNITVLMSDMRGIKSYQIRQDSPHELKLF
                     IVPDSHYTEAVGHHVIDAFRQMTGDGVKLDLEIVPEIAVSESGKRRFIISSVSRGRL"
     gene            complement(1807..2898)
                     /locus_tag="AC16FV_07650"
     CDS             complement(1807..2898)
                     /locus_tag="AC16FV_07650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2849964.1"
                     /translation="MKPASNNSSLPVICLVSELPPPPGGMAVQAEMLTAGLRREGHAV
                     INVPTNPLAHASIWRRIPLLRGMVNLSLFLSRLWPACFKADVVHVFSNSYLSFFLFTF
                     PAVAAGKVMGKRVIIHYHGGSAGAFLKRWFWLARLALETADMIVVPSGFLAAVFLRHH
                     LRTHEVPNILALDNLPFRVRSSLQPHIVMARHLEPDYNVACGMRAFALLRQSHPHATL
                     TIAGDGSEKVMLTSLCHELGVADGVTFTGNVENAQMKTLYDRADIYLNSSRVDNQPVS
                     ILEAFACGLPVVSTAVGGIPYMTRHGEDAMLAPDDDAAQLAAHMAALLRDNALSTRLI
                     HQARQRIQEHSWAPVYSKLLRLYHGEPLT"
     gene            complement(2895..>3800)
                     /locus_tag="AC16FV_07655"
     CDS             complement(2895..>3800)
                     /locus_tag="AC16FV_07655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2849965.1"
                     /translation="MLATQKFDLIYERYAFFLVAGAVLARRHGIPFVLEANEVSGIAH
                     RARPQSYPRLCAWFERILFARCTGIHTVSSRLKQMILRQGIPDGRVHVAPNAFDVAKI
                     KTHGKNPALIEQYRLQNKLVLGFAGWFDRWDRLDFFVEVFRELRKNHDNLHLLLIGDG
                     PVLADVRKIIAHDNLAAHVTLTGPVTRDKIYEYLSLIDIAVLPHSNDFGSPVVMFEFM
                     GLKIPVVAPRLPPIMDVHTNGKTAMLFDPLGVEQCRNAIEQLVTAPELGHQIAQRAYQ
                     KLQTDHTWHCNAALILESAGLASTA"
BASE COUNT          791 a         1060 c         1209 g          740 t
ORIGIN      
        1 cggctgtcca ccgaccagaa aatgaagcgg aatccgcgcc gcgccagcgc cagaaacgac
       61 ggcccggtga tattgccata gggtggccgg aatatcttgt cgatgcgccg acccagtatg
      121 ttttccaaca cgttctgagc gagcattgtt tcttcgacgt aggcgcgcag attcgagcgg
      181 cgcgcatcga gatgcgaata accgtgattg ccgatctggt gcccacgtgc tgcgactttg
      241 cgcaccagcg ccggatgctg ttccgcgatt ttcccctgta aaaaaaacgt ggcgcttgcg
      301 ccggcggcat cgagtatatc gaggatgcgc ggagtatgcc gcgggtccgg gccgtcatcg
      361 aaggtgatgg cgatatctcg tttggcgcgt ctttgctgga tcagtaccaa gctagggaac
      421 acggccgcca gaccgtgttt gattattctg cccgcgctca ttagagccgc ccccggctga
      481 cggaagaaat gatgaagcgg cgcttgccgg attcggatac ggcaatttcc ggcacaatct
      541 cgagatcaag tttgacgccg tcgcccgtca tttgtctgaa ggcatcgatg acatgatgtc
      601 cgaccgcttc ggtgtaatga ctgtcgggca cgataaacaa tttgagttcg tgcggcgaat
      661 cctgccgaat ctggtaggac ttgattccgc gcatgtcgct catcagcacg gtgatgttgg
      721 gggtggtcag caggcggtcg gggagcacca ggatgtccgc aatccggccc tcgatcctgg
      781 ccagccgcgg cagcgtgaca ccacaggggc agggggcgtc gtcgcccatc acacccacgt
      841 cgccgatttc ataacggatg aacgggaaaa ccaggttgga caggtcggtg atgagtatcc
      901 gtcccagctc gccggggcgg caggggcggt tttcccggtc aacggtctcg accagcagga
      961 catcggcatg cagatggtag ccgcggtgct cgtggcactc ggcgcccatg tacatctccc
     1021 ggctgccgta gtggtcatgc accttattgt ccccgaatac ctgattgatc aggccgcgtt
     1081 gttccggtcg cagggtctcg gaactggtga tgagggcgcg gaggaaatca aacgacgtgc
     1141 ccgggtggcg ttttcggatg taatccgcga aggcatgaat gccactggca taaccccaga
     1201 tactggtcgg gcgccaccgg catatgccct ggtaataacg ttcgagcaga tcctcggtca
     1261 ggaaggcgac ggaataatcg cggtaacgct gcagaaaata cgtgagacga tgcagtaatt
     1321 tctgggcatt gttgtagtcg aagtgcgagc ctgacatctg cacggtgcgc tcgcccaggg
     1381 ccacgccggc ccattccgcg ctgcgataga cgctggcgcg gttccagtcc atgccgcgct
     1441 tgtcccagaa ataataggtt ggttcgccgg tggaaccgcc ggtgcgggcc agcacgaagc
     1501 gcgagcgcgg catgttacga cactgcaaac cgttgaagtt ctcctgaatc agggttttgg
     1561 tgagaacggg aatatgcgcc agatcttcga gtcctttgag cggaagcgga acatcgcgca
     1621 gaagcttccc ataataatcg gagttgtcgc gcgcctgccg cagcagctcg ttgagtcgcg
     1681 ccagctgcca tgaccgcagc gtggcgcggt cccaatgctg cgatcggcgc aggaaatgca
     1741 gccgctcgat ggtgcgccgg ccgcggagga aatccaggct tcggaagaat acgtcctgat
     1801 acaggttcat gtcaggggtt caccgtgata aagcctcagc agtttactat agacaggcgc
     1861 ccaggaatgt tcctgtatgc gctgccgggc ctgatgaata aggcgcgtgc tgagcgcatt
     1921 gtcccgcagc agggcggcca tgtgtgccgc gagttgcgcc gcgtcgtcgt ccggcgccag
     1981 catggcgtcc tcgccgtgcc gggtcatgta ggggatgccg cccaccgcgg tactgaccac
     2041 cggcagcccg caggcaaagg cttccaggat ggataccggc tggttgtcga cgcgcgagga
     2101 attgagatag atgtcggcgc gatcgtacag tgtcttcatc tgtgcgtttt cgacatttcc
     2161 cgtgaaggtg acgccatcgg caacgccaag ctcgtgacac aatgaggtca gcatgacctt
     2221 ctcactgccg tcgccggcaa tggtaagcgt ggcgtgggga tgggactgcc gcagcagtgc
     2281 aaaggcccgc ataccgcacg cgacgttgta atcaggctcc agatgccggg ccatgacgat
     2341 atgcggctgg agcgaagaac gcacgcggaa gggaagattg tcgagcgcca gtatattggg
     2401 aacttcgtgg gtgcgcaggt gatggcgcag aaataccgcg gccaagaaac cggagggaac
     2461 gacgatcatg tcggcggttt ccagcgccag ccgcgccagc cagaaccagc gtttaaggaa
     2521 cgcgccggcc gatcctccgt gataatgaat aatgacgcgc tttcccatga ccttgcccgc
     2581 ggcgacggcg ggaaaggtga aaagaaagaa actcaggtaa gaattgctga agacgtgcac
     2641 gacatccgcc ttgaagcagg ccggccacag gcgcgacaag aacaacgaca ggttgaccat
     2701 gccgcgcagc agcgggatcc ggcgccagat tgaggcatgt gcgagcggat tggttggcac
     2761 attgatcaca gcgtgacctt cgcggcgcag gccggcagtc agcatttcgg cctgtactgc
     2821 catgccgccc ggaggcgggg gcagctccga taccagacat atgaccggca gtgagctgtt
     2881 gttgctggcc ggtttcatgc agtcgaggcc aggccggccg attccagaat cagcgcggca
     2941 ttgcagtgcc aggtgtgatc ggtttgaagt ttctgatagg cccgttgtgc gatctggtga
     3001 cccaattccg gagccgtgac aagctgctca atcgcattcc ggcactgctc gacacccagc
     3061 ggatcgaaca gcatggcggt tttgccgttg gtgtgcacgt ccatgatcgg cggcagacgc
     3121 ggcgcgacca cgggaatttt gagacccatg aattcgaaca tcaccacggg cgaaccgaaa
     3181 tcgttggaat gtggcagtac cgcgatatcg atcagggaaa gatattcgta gattttatcg
     3241 cgcgtcaccg gcccggtaag ggtgacgtgc gcggccagat tgtcgtgagc gatgatcttt
     3301 ctgacatcgg ccagtaccgg gccgtctccg atcagcaaca ggtgcaggtt gtcgtggttc
     3361 ttgcgcagtt cgcgaaagac ctcgacgaag aaatcgagcc ggtcccagcg gtcgaaccag
     3421 ccagcaaagc cgagcaccag tttgttttgc agccgatatt gttcgatcag ggccggattc
     3481 tttccatgcg ttttaatttt ggcgacatcg aaggcattcg gcgccacgtg gacgcgcccg
     3541 tcgggaatgc cctgacgcag aatcatctgt ttgaggcgcg aggatacggt gtgaatcccg
     3601 gtgcagcgcg caaacagaat gcgctcgaac caggcgcaca ggcgcggata ggattgcggt
     3661 cgcgcgcgat gcgcgatgcc gctgacttca tttgcttcca gcacgaaagg tatcccgtgt
     3721 cgacgtgcca gcacggcgcc ggctaccaga aaaaacgcgt agcgctcgta gatcagatcg
     3781 aatttctgtg tcgccaacat
//
LOCUS       DBNGZE010000295         4026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1361742, whole genome shotgun sequence.
ACCESSION   DBNGZE010000295 DBNGZE010000000
VERSION     DBNGZE010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1361742"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..449
                     /gene="rpsF"
                     /locus_tag="AC16FV_07660"
     CDS             39..449
                     /gene="rpsF"
                     /locus_tag="AC16FV_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR2849966.1"
                     /translation="MRHYEVVFMVHPDQSDQVPAMIERYRSMIESAQGAIHRLEDWGR
                     RQLAYPLNKVHKAHYVLMNIECELGTLRELESAFRFNDAVLRSLVIKRKHAITSPSPL
                     VKENEEKSAAPVAAAADTDNGDSEAAESAAPDQA"
     gene            461..685
                     /gene="rpsR"
                     /locus_tag="AC16FV_07665"
     CDS             461..685
                     /gene="rpsR"
                     /locus_tag="AC16FV_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR2849967.1"
                     /translation="MSRFFRRKKFCRFTAEKVEEIDYKDIAVLSGYITETGRIIPCRN
                     TGTKARYQRQLTRAIKYARYLALMPYCDSH"
     gene            709..1158
                     /gene="rplI"
                     /locus_tag="AC16FV_07670"
     CDS             709..1158
                     /gene="rplI"
                     /locus_tag="AC16FV_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR2849968.1"
                     /translation="MEVILLEKVRNLGNLGDQVKVKSGFARNYLIPYGKAVTANEQNR
                     AKFEAQRAELEKAQADAFGKAQARAEKMTGFTVQIVRKVSEEGKLFGSVGIRDICDAV
                     EQAGFELARSELHLDRGPLKEIGDHEVSVSLHPEVNFKIIVSVIGEK"
     gene            1274..2665
                     /gene="dnaB"
                     /locus_tag="AC16FV_07675"
     CDS             1274..2665
                     /gene="dnaB"
                     /locus_tag="AC16FV_07675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR2849969.1"
                     /translation="MEERAFSRRGTESVADLLKVPPQSIEAEQSVLGGLLLDNQSWDR
                     IADILTADDFYRREHRLIFGAIAALCEENSPADVVTVSEWLERSGELEPAGGLSYLGS
                     LANNTPSAANIVAYAAIVRENSVMRELVRAAGEISTAAYTPEGRSATELLDFAEKRIM
                     DISEKGHRRGEFQPLNNLLSKAVDRIDELFRSKSSITGVPTGFTDLDDMTSGLQRSDL
                     VIIAGRPSMGKTSLAMNIAENAAVGHKIPVAVFSMEMPGTQLALRMMASLGRINAHRV
                     RTGKLDDDDWPRLTSAVSLLNEAPIFIDDSPGLTPMELRASARRLKREHGLGLIIIDY
                     LQLMQSTERMEENRATEISNITRALKGLAKELDVPVIAMSQLNRSVESRTDKRPVMSD
                     LRESGAIEQDADVIFFIYRDEVYNKDSAVKGTADIIIGKQRNGPIGDVRLTFLGEYTR
                     FENYTSAGFDGGF"
     gene            2806..3882
                     /gene="alr"
                     /locus_tag="AC16FV_07680"
     CDS             2806..3882
                     /gene="alr"
                     /locus_tag="AC16FV_07680"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR2849970.1"
                     /translation="MSRPARALLDAGALKHNLQQVRLHAKHSRVMAIVKADGYGHGLA
                     WVARTLGGSADAFGVSSIEEGLQLREAGIAQPICLLEGFFTTDELPLLSKQRLEPVIH
                     HETQLKVLAQASPLLRLTVWVKVDTGMHRLGFPPAAVPDVLKQLRSSRAVTGVRIMSH
                     FARAEFADDDVTQSQISDFASLIRNLELETSLANSAGILAWPSSHLDWVRPGIMLYGA
                     SPLMDKDAATLDLKPVMTLESALIAVQPRKKGDAIGYGGDWRCPEDMPVGVAAIGYGD
                     GYPRHAPPGTPVLLNGRRVPTVGRVSMDMITLDLRTQPQAKVGDPVVLWGRDLPVEQV
                     AESAGTVSYELLCHVTERIPRVVI"
BASE COUNT          846 a         1231 c         1194 g          755 t
ORIGIN      
        1 gcagcggaag gctgaagttt atccataagg agtgttgcat gcgtcattat gaagtcgttt
       61 tcatggtcca cccggaccag agtgatcagg tgcccgcgat gatcgagcgc taccgctcga
      121 tgatcgaatc cgcccagggc gcgatccacc gcctcgaaga ctggggccgc cgtcagctcg
      181 cttatcccct gaacaaggtg cacaaggcgc actacgtgct catgaacatc gagtgcgagc
      241 tcggaacctt gcgcgaactg gagtcggcgt tccgcttcaa cgacgcggtg ctgcgctcgc
      301 ttgtcatcaa gcgcaagcac gccatcacca gcccgtcgcc gctcgtcaag gaaaacgaag
      361 agaagtccgc ggcgcccgtg gcggcagcgg cggataccga taacggcgac agcgaagcgg
      421 ccgaatccgc cgcacccgat caggcttaag gagcacaacc atgtcacgtt ttttccgacg
      481 caagaaattc tgccgtttca cggccgagaa agttgaagag atcgattaca aggatatcgc
      541 ggtgttgagc ggttacatca ccgaaaccgg ccgcatcatc ccgtgccgca ataccggcac
      601 caaggcccgc taccagcggc agctgacgcg cgccatcaag tacgcgcgct atctggcgct
      661 gatgccctac tgcgacagtc actgaacgaa tacggcgagg tcttaacgat ggaagtcatt
      721 ctgctggaaa aagtgcgcaa cctgggcaac ctgggcgatc aggtcaaggt gaagtcgggt
      781 ttcgcgcgca actatctcat tccctacggc aaggcagtca ccgccaacga gcaaaatcgc
      841 gccaagttcg aggcgcaacg cgccgagctt gaaaaggccc aggccgacgc gttcggcaag
      901 gcgcaggcgc gcgccgagaa gatgaccggc tttacggtcc agatcgtgcg caaggtcagc
      961 gaagagggca aactcttcgg ttccgtcggc atccgcgaca tttgcgacgc cgtggagcag
     1021 gccggtttcg aactggcccg gtccgaactg catctcgacc gcggtccgct caaggaaatc
     1081 ggcgatcacg aagtttccgt gtcgttgcat cctgaagtca acttcaagat catcgtttcc
     1141 gtcatcggcg agaaatagcc cgtaatttcc gagccgcagc gcatctgttc aacaggatgc
     1201 gctgcgttat aatgcgtgac gcaagtgccg cgaggtagag aaacggcaat ccagggtcga
     1261 gagggggagg tcggtggaag aacgagcctt ttccaggcgt ggaacggagt cggtggccga
     1321 tctgctcaag gtcccgccgc aatccattga ggccgagcaa tccgtgctcg gcggtctgct
     1381 gctcgacaac cagagctggg accgtatcgc cgacattctc accgccgacg atttttaccg
     1441 ccgcgaacac cgcctgatct tcggcgccat cgccgccctg tgcgaggaaa acagcccggc
     1501 ggacgtggtc acggtttccg aatggctcga acgcagcggc gagctggaac ccgccggcgg
     1561 tctgtcctac ctcggctcgc tcgccaataa tacccccagt gccgccaaca tcgtcgcgta
     1621 cgccgccatc gtgcgcgaga actcggtcat gcgcgaactg gtgcgggcgg ccggggaaat
     1681 cagtaccgcc gcctatacgc ccgagggccg ctccgccacc gagctgctgg attttgccga
     1741 aaagcgcatc atggatatca gcgaaaaggg ccaccggcgc ggcgaattcc agcccctgaa
     1801 caacctgctc agcaaggccg ttgaccgcat tgatgagctg tttcgttcca agtcctccat
     1861 caccggcgtg cccacgggct ttaccgatct cgacgacatg acctccggcc tgcagcggtc
     1921 ggatctcgtc atcatcgcgg gccggccgtc catgggtaaa accagccttg ccatgaatat
     1981 cgccgaaaac gccgccgtcg gccacaagat cccggtggcg gtgttcagca tggaaatgcc
     2041 gggcacgcaa ctggcgttgc gcatgatggc ttcgctcggg cgcatcaacg cgcaccgggt
     2101 acgcaccggc aaactggacg atgacgattg gccgcgcctg acctcggccg tgagcctgtt
     2161 gaacgaggcg ccgatcttca tcgacgattc cccggggctg acgccgatgg aactgcgcgc
     2221 cagcgcgcgc cgcctgaagc gtgagcacgg ccttgggctg atcatcatcg attatttgca
     2281 actcatgcag tccaccgagc gcatggaaga gaaccgcgcc accgagattt ccaatatcac
     2341 gcgcgcgctc aagggcctgg ccaaggagct ggacgtgccg gtgatcgcca tgtcgcagct
     2401 caaccgcagc gtcgaatccc gcacggacaa aaggccggtc atgtccgatc tgcgcgaatc
     2461 cggcgccatc gagcaggacg ccgacgtgat cttctttatc tatcgcgacg aggtctacaa
     2521 caaggacagc gcggtcaagg gcaccgccga cattatcatc ggcaagcagc gtaacggacc
     2581 gatcggcgat gtgcgcctga ccttcctcgg ggaatacacg cgctttgaaa actatacttc
     2641 cgccggcttc gacgggggtt tttaatattt tttatccgca gataaacgca gatataaaaa
     2701 acggatgaac gcagataatc caggagcaga tatgccaaat ctgcgtttat cttcttatgc
     2761 atccgtgtgc atctgcggat gccttgatct taagatggct tctggatgag ccggccggcg
     2821 cgcgcgctgc tggacgccgg ggcgctcaaa cacaatctgc aacaggtccg gcttcacgcg
     2881 aaacactcgc gcgtgatggc catcgtcaaa gccgacggtt atggtcacgg actcgcttgg
     2941 gtcgcgcgca ccctgggcgg atccgccgat gccttcggtg tctccagcat cgaagaggga
     3001 ctgcaactgc gcgaagccgg cattgcccaa cccatctgcc tgctcgaggg tttcttcacc
     3061 accgacgagc tgccgctgct gtcgaagcag cgtctcgaac cggtgattca tcatgagacc
     3121 caactcaagg tcctggcgca ggcttcacca ttgctgaggc tcacggtctg ggtcaaggtc
     3181 gataccggca tgcaccgcct cgggtttccg cccgctgccg taccggatgt cctgaaacaa
     3241 ttgcgcagtt cccgtgccgt aaccggcgtg cgtatcatgt cgcatttcgc gcgcgcggaa
     3301 tttgccgacg atgatgtcac ccagtcacag atcagtgatt tcgccagcct gatccggaac
     3361 ctggaactgg aaacgagcct cgccaattcc gccggcatcc tcgcctggcc gtcgagccat
     3421 ctcgactggg tgcgccccgg catcatgctt tacggcgctt caccgctgat ggacaaggac
     3481 gcggcgacgc tggatctcaa acccgtcatg acgctcgaat cggcgctgat cgcggtgcag
     3541 ccgcgcaaaa agggcgatgc catcggctac ggcggggatt ggcgctgccc ggaggacatg
     3601 ccggtgggcg tggcggcgat cggttatggc gacggttacc cgcgccacgc cccgccggga
     3661 acgccggtgc tgctaaacgg ccgccgtgtg ccgaccgtgg gccgggtttc catggacatg
     3721 atcacgctcg acctgcgcac ccagccgcag gccaaagtgg gcgacccggt ggtgttgtgg
     3781 ggtcgggact tgccggtgga acaggtggcg gaaagtgcag ggactgtttc gtacgaattg
     3841 ttgtgccacg tgaccgagcg catcccgcgc gtggttatat gatttttatc cgcagatgaa
     3901 cgcggatgta taagaagata aacgcagatt tttatataag ttcctaaatt atctgcgttc
     3961 atccattttt atatctgcgt taatctgcgt tccaaaatat ttgaatcatg gccaaggtca
     4021 aaataa
//
LOCUS       DBNGZE010000296         1773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_737093, whole genome shotgun sequence.
ACCESSION   DBNGZE010000296 DBNGZE010000000
VERSION     DBNGZE010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_737093"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..571
                     /locus_tag="AC16FV_07685"
     CDS             92..571
                     /locus_tag="AC16FV_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR2849971.1"
                     /translation="MSIAHAVPAVTHGSSRWLLLGSLALNLFFVGVAVAMAIRTPAPS
                     YWDPNVFVRVERLAATLPPADADIVRGQINENHAAIDDAQTKYHSARQHIHDVLRQEP
                     FDVEAMRAATAQTRAARLAFDQTVQGVFANIAAKISPAGRQALANWPPGRRPVSDKR"
     gene            684..>1773
                     /locus_tag="AC16FV_07690"
     CDS             684..>1773
                     /locus_tag="AC16FV_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR2849972.1"
                     /translation="MLHGLCLTVPLGGCLLTGDKPEPGLDIPPAYTFGPRKPVAAEAA
                     VPPLDWWRAFRSRELTEIIEAAREGNLDIAAAVARIVQADANARIAGAALLPIIDFNG
                     NAEHSQQSKTTGSTNVISVSPNSRPSVAIINNNLRATLSVTSYEIDFWGKNRSAVRAA
                     EETAVATRYDREVVSLSSVVAAANAYFQVLAAQDRLRVAHQNLESATRVLNLIQQRLQ
                     AGTASALDTAQQESLVNNLRATIPPLEQTLRLNRVTLAVLMGRSPESVTIRGGSLRSI
                     AYPRVTPGLPSELLAQRPDIREAEANLAAANANLVNARAQFLPSIKLTGEGGYESAVL
                     KLLLRPESVIYTAAASLTQPIFHGGELLG"
BASE COUNT          337 a          577 c          538 g          321 t
ORIGIN      
        1 ttgacggtcc gggtgcgccg cccgcttttg tcggcggcgg cggtatcggc gcgatcacgt
       61 tcgagcccga cgcattcact ggaggccggc catgagcatc gcccatgccg ttccggccgt
      121 gacgcatggc tcttcgcgct ggctgctgct cggctcgctc gcgctcaacc tgtttttcgt
      181 gggcgtcgca gtggccatgg cgatccgcac gccagcgccg tcttattggg atcccaatgt
      241 gttcgtgcgc gtcgagcgat tggcggcgac gctgccgcct gccgacgctg acatcgtgcg
      301 cggccaaatt aatgaaaacc acgcggccat cgacgatgcg cagacaaaat accattctgc
      361 gcgccaacat atccatgacg tgctgcggca ggaacccttc gacgtcgagg ccatgcgcgc
      421 ggcaaccgcc cagacgcggg cagcacggct tgccttcgac cagacggtcc agggcgtgtt
      481 cgccaatatc gcggcgaaaa tctcgccggc tggccggcag gctttggcca attggccccc
      541 aggacgcaga cccgtcagcg acaaacggta gtacatttag taaacgagtc gctgttggcc
      601 gggttctgaa agtcttggga atgcaagggg ccgggaaggc aaaggaggcg ggaacgaaag
      661 tgcgggggcg cccgttttgg attatgcttc atggactatg cctgacggtg cccctcgggg
      721 ggtgcctgct gaccggcgac aagccggagc cggggctcga tattccgccg gcctatactt
      781 tcggtccgcg taagcctgta gcggcagaag cagcggttcc cccactcgac tggtggcgcg
      841 ccttccgctc gcgcgaattg actgagatta tcgaagcggc gcgcgagggc aatcttgata
      901 tagccgcagc ggtcgctcgc attgtgcagg cggatgccaa tgcccgcatc gccggcgctg
      961 cacttttgcc gatcatcgat ttcaacggca acgccgagca cagtcaacag tccaagacca
     1021 ccggcagcac gaacgttatc agtgtttcgc caaactcgcg ccctagcgtc gctatcatca
     1081 acaacaacct gagggccacg ctcagcgtca ccagctacga aatcgacttt tggggcaaga
     1141 accgttccgc ggtgcgcgcg gccgaagaga ccgcggttgc cacccgctat gaccgcgagg
     1201 ttgtcagtct cagctcggtg gtcgccgccg ccaacgccta tttccaggtg ctggccgcgc
     1261 aagaccgcct gcgtgtcgcg caccagaatc ttgagagcgc gactcgcgtg ctcaacctaa
     1321 tccagcagcg gcttcaggcg ggcaccgcgt ctgcacttga tacagcgcaa caggaaagtc
     1381 tcgtgaacaa cctgcgcgcc accattccgc cactcgaaca gacactgcgg ctaaaccggg
     1441 tcacgctcgc ggtgctgatg gggcgttcgc ccgagagcgt gactatccgc ggcggaagct
     1501 tacgtagtat cgcctatcca cgcgtgacac cgggcctacc atccgagctt ctggcgcagc
     1561 gtcccgatat ccgcgaagcc gaagccaatc tcgccgccgc caacgccaac ttggtgaacg
     1621 cgcgcgcaca attcctgcca agcatcaaac tcaccggcga aggtggctat gagagcgcgg
     1681 tcctcaaact gttgctgcgg ccggaatcgg tcatttacac ggccgctgcc agcctcactc
     1741 aaccgatttt ccatggtgga gagcttctcg gca
//
LOCUS       DBNGZE010000297         4979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_806690, whole genome shotgun sequence.
ACCESSION   DBNGZE010000297 DBNGZE010000000
VERSION     DBNGZE010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_806690"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="AC16FV_07695"
     CDS             complement(<1..1152)
                     /locus_tag="AC16FV_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2849973.1"
                     /translation="MLRLTEIKLDLDHPEAALKTAILRTLGIPEPELLGYRLRRRGYD
                     ARKSGEILFVYTLDVEVKDETVLLKRFHHSHHITRAPDETYRYPAQALANLKTRPVVI
                     GTGPCGIFAGLSLARMGFRPIILERGKSVRERTQDVWGFWRERHLNPESNVQFGEGGA
                     GTFSDGKLHTGIKDRGHHIRQILKDFVDAGAPEEILYINKPHIGTLRLVKVVENLREK
                     IIELGGEIHFQSHVTDIAVENGCVRGVTLAGGEQIATDHVVLAVGHSARETFEMLHSR
                     GVHIEPKPFSIGFRIEHPQHMIDVCRLGKHAGNPVVGAADYKLVHHCKNGRDVYSFCM
                     CPGGQVVAATSEEGRVVTNGMSHYSRNERNANAGIVVGITPRDFGSDHPL"
     gene            complement(1372..1686)
                     /locus_tag="AC16FV_07700"
     CDS             complement(1372..1686)
                     /locus_tag="AC16FV_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HIR2849974.1"
                     /translation="MNRPEYASEIDAMRDLRQRGFTTDLEFRDGALRETTTGRRFRPD
                     ELAIVEHHRFEGESDPEDMSVVYAIESEDGVRGIVVDAFGAYADPSLAAFLEKVKVHE
                     HV"
     gene            complement(1860..2162)
                     /locus_tag="AC16FV_07705"
     CDS             complement(1860..2162)
                     /locus_tag="AC16FV_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849975.1"
                     /translation="MLRARRRVVVRLGWPVRLMVVAGRDMNGVRLAASGNHGKYRRQR
                     RSRHDHGQQQRQCAFDSEATDHAPNFSRCWFPVKPDETRPRSSDQPATFRYIVWGL"
     gene            2424..4124
                     /locus_tag="AC16FV_07710"
     CDS             2424..4124
                     /locus_tag="AC16FV_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HIR2849976.1"
                     /translation="MSTRRHFVATPNLPRRRFIQGLAAGGILLGLSPWLRSARAQDAA
                     GTSIGTAKVYSGTEFDLTIAETPVNFTGAPRMATTINGSIPAPTLRWRQGDTVTLRVT
                     NRLPVDTSIHWHGILLPYQMDGVPGISFRGIAPGETFTYRFKVAQTGTYWYHSHSGMQ
                     EQTGMYGAIIIDPPARDSVRADREYVVLLSDWTDEDPMRIHAKLKMQSDYYNFNQSTA
                     GDFMRDVSRDGLRSAFYKRQMWQQMRMNPTDLADISGYTYTYLMNGVTPAGNWNGLFR
                     PGEKVRLRFINGSAMTFFDVRIPGLKMTVVQTDGQDVDAVTVDEIRIGVAETYDVIVT
                     PRDEAYTIFAQSMDRTGFARGTLAPRAGMTAAVPDLDKPEPLSMGDMMGDMMGGKRAH
                     HARTEYGPSVDMRVDTPRINLDDPGIGLRNNGRRVLTYADLRTIGGPLDKRGPGREIE
                     LHLTGNMERYTWSLDGLEFGRSTPVHFRRDERLRVILVNDTMMTHPMHMHGMWQELES
                     PDGRFQVRKHTINVQPAQRISFMVSADALGRWAWHCHLLYHMDSGMFREIVVARAHNG
                     "
     gene            4223..>4979
                     /locus_tag="AC16FV_07715"
     CDS             4223..>4979
                     /locus_tag="AC16FV_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HIR2849977.1"
                     /translation="MPEMDHSQMDHNKMDHGAAPAPDPHAGHDMGGMDHGSMQGGSAP
                     PDARDPHAYSGGYDFGPIPPPHMGDVHNFYGLRVDRFERVRTRENSYTEYDLQAWYGR
                     DYDRAVLKAEGEIDGGEHHELSTELLWSHAVAAYWNTQLGVRHDGGEEPSRAWLAFGV
                     QGLAPYWFEMDITGYLGEHARTALGIEAEYELLLTQRLILQPRIEANVYGKRDAEREL
                     GSGLSDLTAGVRLRYEIRREFAPYIGIERSGKFG"
BASE COUNT          911 a         1532 c         1555 g          981 t
ORIGIN      
        1 gagtggatgg tcgctcccga agtcccgtgg cgtgatgccg acaacgatgc cggcgttggc
       61 gttgcgttca ttgcgcgagt aatggctcat gccattggtg acaacacgcc cttcctccga
      121 ggtggctgcc accacctgcc cgccgggaca catgcagaaa ctgtaaacgt cacggccgtt
      181 tttgcagtga tggaccagtt tgtaatcggc cgcgcccaca accggattgc ccgcgtgttt
      241 tcccagacgg catacatcaa tcatgtgttg cgggtgttcg atgcgaaagc cgatggaaaa
      301 gggcttgggt tcgatgtgta ctccacggct gtgcagcatt tcaaaggtct cacgcgcgct
      361 gtggccgacg gcgaggacga cgtggtcggt ggcaatctgc tcgccgccgg cgagcgttac
      421 gccgcgcacg caaccatttt caacggcgat atccgtgacg tggctttgga agtggatctc
      481 gccgccaagc tcgatgattt tctcgcgcag attctccacc actttcacga gccgcaaggt
      541 gccgatatgc ggcttgttga tgtagaggat ttcctccggc gcccccgcgt ccacgaagtc
      601 cttcaatatt tgccggatat gatgcccacg gtccttgatg ccggtgtgca gcttgccgtc
      661 ggagaaggtg ccggcgccac cctcgccgaa ctgcacgttg gattccggat tcagatgacg
      721 ctcgcgccag aagccccaca cgtcttgcgt gcgttcacgc acgctcttgc cgcgttcgag
      781 gatgataggg cggaagccca tgcgcgcgag cgacagcccg gcgaatatcc cgcacgggcc
      841 ggtaccgatg accaccggcc gggttttcag attagccagt gcctgcgccg gatagcggta
      901 agtctcgtcc ggcgcgcggg tgatgtgatg gctgtgatga aagcgcttga gcagcacggt
      961 ttcgtctttg acctcgacgt cgagggtgta gacgaacagg atctcgccgg atttgcgcgc
     1021 gtcatagcca cgacggcgca agcggtagcc gagcagttcc ggctcgggta tgcctaaagt
     1081 gcgtaggatc gcggtcttga gggcggcctc ggggtggtcg aggtcgagtt tgatttcggt
     1141 cagtcgcagc acgatttttt tgcaggcggc gcggcggtat cgtccatgcc gtgtgggcaa
     1201 cgcagtataa gtcaattccg tccttcggtc cgtaagcgcg cctgctcaaa cgacaggacg
     1261 aacgagcgaa gcgagcaggg cttccgtctc ggcgcgcttc ccggttgcgc tgccgatgcc
     1321 cggggtcctg tttccattcg gatgaaatag tgtgcctggt ttaaccgcac actagacgtg
     1381 ctcgtgcact ttaacctttt ccaaaaaagc cgcgaggctg ggatcggcgt aggcgccgaa
     1441 ggcgtccacc acgataccgc gcacgccgtc ttcgctttcg atggcgtaca ccaccgacat
     1501 gtcctcggga tcgctctcgc cctcgaaccg gtgatgctcc acgatggcga gctcgtcggg
     1561 tcggaacctc cgcccggtgg tggtctcgcg cagagcgccg tcacggaact cgaggtcggt
     1621 ggtaaagccg cgctgacgca gatcgcgcat ggcatcgatt tcgctggcgt attcaggtcg
     1681 attcatgtgg cctcctttcc gtcctttggt ccgtaagcgc gccatccttg gcgcacttct
     1741 acatgggatg gccggggtgt ggcgacgggt gcttgccttg cgccttggca agacacggat
     1801 gacatcaatt aaacacctaa atctgtcctc cgtcgaaggg tcagcgccac ccgaacgact
     1861 caaagacccc acacgatgta tcgaaacgtc gcgggctgat cgctggaccg cggccgggtc
     1921 tcgtccggtt ttaccggaaa ccaacatctg ctaaagttcg gagcatggtc cgtcgcttcg
     1981 ctgtcaaacg cgcattggcg ctgctgttgg ccgtggtcat ggcggctacg ccgctgtcgc
     2041 cggtatttgc cgtggttgcc ggaagcggcg aggcgcacgc cgttcatgtc tcgcccggca
     2101 acgaccatca ggcggacggg ccagcccagg cggaccacca ctcgacgacg tgcacgcagc
     2161 atgattcctc cgccggccac gatcgcgact gctgcgcgca ttgcttcggg ctggtatctc
     2221 tcgtgcaggc agcgtatctc cactcgcatc cagtgcaaac accgatcttg actgagctcc
     2281 atccccgcct tctggcgacg tctccggacc gaccgccgcg cattctcccg ctctgattct
     2341 ccgttgtttc tgacgctgta aggcgtcgcg ccgccaccag cggcgtatcc ggtgtttcat
     2401 ttcatgcatt gcgggagagt gttatgtcta cacgtcgtca ttttgtggcc acgccgaatt
     2461 tgccgcgccg gcgatttatt cagggtttgg cggctggcgg catcctgctg ggactgtcgc
     2521 cctggctcag gtcggcccgg gcgcaagacg cagccggaac atccatcggg acggccaagg
     2581 tgtattccgg caccgagttc gatcttacga tcgccgaaac gccggtcaat ttcaccggcg
     2641 cgccgcgcat ggccaccacc atcaatggtt cgattccggc gcccactctg cgctggcggc
     2701 agggagatac cgtcaccctg cgcgttacca accggctgcc cgtggacact tccatccatt
     2761 ggcacggcat cctgctgcca taccagatgg atggtgtgcc gggcatcagt ttcaggggga
     2821 tcgcgccggg ggaaaccttc acttatcgct tcaaggtcgc gcagaccggt acctattggt
     2881 accactccca ttccggcatg caggagcaga ccggtatgta tggcgccatc atcatcgatc
     2941 cacccgccag agattccgtt cgtgccgacc gggaatatgt ggtgctgttg tccgactgga
     3001 ccgacgaaga cccgatgcgt attcatgcca agctcaagat gcagagcgat tattacaact
     3061 tcaaccagtc cacggccggc gatttcatgc gcgacgtatc ccgggatgga ctgcgatccg
     3121 cgttctacaa acgccagatg tggcagcaga tgcgcatgaa cccgaccgac ctggctgaca
     3181 tcagcggcta tacctacact tatctcatga atggcgtcac gccggcgggc aactggaacg
     3241 gcctgttccg tccgggcgag aaggtgcggc tgcgcttcat caacggttcc gccatgacgt
     3301 tcttcgacgt gcgcatcccg gggctcaaga tgacagtggt ccagaccgat ggccaggacg
     3361 tcgatgccgt gacggtggac gagatccgca tcggcgtggc cgagacctac gatgtcatcg
     3421 tcactccccg ggatgaggcc tatacgatct tcgcccagtc gatggaccgc accggtttcg
     3481 cccgcggcac cctcgccccg cgcgccggca tgacggcggc agtgcccgat ctggacaaac
     3541 ccgaaccgct ctcaatgggg gacatgatgg gagatatgat gggcggcaag cgcgcgcatc
     3601 acgcccgcac cgagtacggc cccagcgtgg acatgcgcgt ggacacgccg cgcatcaatc
     3661 tcgatgaccc cggcattggt ctcaggaaca acgggcgtcg ggtgttgacc tatgccgacc
     3721 tgcgcacgat cggcgggccg ctggacaaac gtggacccgg acgcgaaatc gagctgcacc
     3781 tcacgggcaa catggagcgt tatacctggt cgctcgacgg cctcgagttc ggccggtcca
     3841 cgccggtgca tttccgtcgc gatgagcgcc tgcgcgtgat cctcgtcaac gacaccatga
     3901 tgacccaccc catgcacatg cacggcatgt ggcaggaact ggaaagcccg gatgggcgct
     3961 ttcaggtgcg caagcacacc atcaacgtgc agcccgcgca gcgcatcagt tttatggtga
     4021 gcgcggatgc cctcggtcgc tgggcttggc attgccatct gctgtatcac atggattccg
     4081 gcatgttccg cgaaattgtc gtggcccgag cgcacaacgg ataatggaga acgaaatgat
     4141 aaacagcaga atgttgtttg ccgccgccgt ggtgatgctc gggatgtccg acgcctgggc
     4201 gcaggagccg gtggccccga cgatgccgga aatggatcac tcgcagatgg accacaataa
     4261 aatggatcac ggcgccgcgc cggctccgga cccgcacgcg ggtcacgaca tgggcggcat
     4321 ggaccatggt tcaatgcagg gcggctcggc gccgcctgat gcgcgcgacc cgcacgccta
     4381 ctcgggtggg tacgacttcg gtccgatccc gccgccgcac atgggggacg tgcataattt
     4441 ttacggattg cgggtggacc ggttcgaaag ggtgcgcacg cgcgaaaact cttacacgga
     4501 atacgacctg caggcctggt acggccggga ttacgaccgc gcggtgctca aggccgaggg
     4561 cgagatagat ggcggtgagc accatgagct gagcaccgag ctgctgtgga gtcacgcggt
     4621 ggccgcgtac tggaatacgc aattgggcgt gcgccatgac ggcggcgagg agccaagtcg
     4681 cgcttggctg gccttcgggg ttcagggtct tgctccttac tggttcgaaa tggatatcac
     4741 cggctacctc ggcgagcacg cccgcacggc gctgggtatc gaggcggagt acgagttgct
     4801 gctgacgcaa aggctgatcc tgcagccccg catcgaagcc aatgtctacg gcaaacgcga
     4861 cgccgagcgt gagttgggtt ccggcttgtc cgacctgacg gccggcgtgc gattacgtta
     4921 cgaaatccgg cgtgaattcg ctccttacat cggcatcgag cggagcggca aattcggcg
//
LOCUS       DBNGZE010000298         6276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1431812, whole genome shotgun sequence.
ACCESSION   DBNGZE010000298 DBNGZE010000000
VERSION     DBNGZE010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1431812"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..497)
                     /locus_tag="AC16FV_07720"
     CDS             complement(93..497)
                     /locus_tag="AC16FV_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849978.1"
                     /translation="MNRKILSTAVLFLAVVMAAPLAQARDGRDGWDGRDHYRPAPVYR
                     EKYVERHYYHPGQHRGWYKPHHYYRPAPRHHYRHDHGYYPAHRTSSSAPIILGSVIGG
                     VVGHEIGYGDPGHTAAGAVIGTVIGYEIARNR"
     gene            complement(635..1939)
                     /locus_tag="AC16FV_07725"
     CDS             complement(635..1939)
                     /locus_tag="AC16FV_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2849979.1"
                     /translation="MNRPRVKTASPADVHARLRRLRWTAFALVAMSYMLAFFHRMAPA
                     AIASDLQQAFNASATALGGLAATYFYVYTVMQIPAGVLADTLGPRRIVTLGGVIAAVG
                     SLLFGYATTITEASIGRMLIGLGVSVAFIALLKLNAVWFHDRHFGTAVGLTLVLGNVG
                     SVLAAAPLAWVLQFTTWRAVFIVLGGVSFVLAVFTWLLVRDHPGEAGLPSLREMDGHA
                     AHPPHEGHWFDGLMTVLRNRYTWPIFGMNLGIGGSFIAFAGLWAVPFLRGVYGMERVV
                     ATQHTSLMLAGFAVGAFLVGLVSDRLGRRRPVLITTALAHLLCWLPLVLALPLSLATS
                     YSLLLLMGLSAGSFTLSWACVKEVNPHALSGMATSVVNTGVFLGTGILQPLVGWTIDR
                     AGQAAGAAAQYRPGMEIFFACAVAGFAGSLRVRETYCRYVRG"
     gene            complement(2046..3248)
                     /locus_tag="AC16FV_07730"
     CDS             complement(2046..3248)
                     /locus_tag="AC16FV_07730"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR2849980.1"
                     /translation="MRIAIRPLAFLFFLCLVVAEGWAAPEPLTYEEQALMVDGKRQLV
                     RVPKGFRLELLTDRLDGPRLPTFADSGDLFIGSKSGRVYRLPPPYTAPGVLVRLDDYP
                     HSVAFRKGEILIARTNGVYRASYLPGQKQIAPESVKLLARLPGGGGHNSRTVGVGPDG
                     RVYVSLGIQGNCSDQYLGDSYPFDERRGGVFVLREEGGKAYWRAYGSGLRNPVGFAWH
                     PVTKVMYASNNGPDHLGYEQPPEYFSRVTEGSFHGMPWFQFDGKQLRRDDCVSSQPPR
                     PLKDIVPPVATFPARNAPMGVAFVPKGALNKALEFDAIVALRGSWGTQPSGGYLGDSA
                     TRRPPKLVAVRFRDGEAMRVDDLVTGFQLRDGERWARPVGVAIGPDGALYFTSDSETH
                     GLFRLRLK"
     gene            complement(3276..4037)
                     /locus_tag="AC16FV_07735"
     CDS             complement(3276..4037)
                     /locus_tag="AC16FV_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR2849981.1"
                     /translation="MKRPIYLSVMAVGVFLTGCVSTSTFEQKSAELAGCETRAKNELA
                     ACNTARGETETKLTAATQELDVYRKAAEANKQKLEAVQQQENQLRERMQKELTDKSVE
                     IEQLRGQLTVRMLDKIVFRSGSADILPEGMAVLDKLVEAIADSTDTIRVEGHTDDTPI
                     SDKLKKKYPSNWELSAARASAVARYIETKHHINPKRLESLGFSMYHPVAPNDTDENKQ
                     RNRRVEIVLKPAAETEAAPTVPAAPAPQDTTGKTQ"
     gene            4330..5166
                     /locus_tag="AC16FV_07740"
     CDS             4330..5166
                     /locus_tag="AC16FV_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2849982.1"
                     /translation="MQRISKHLRIGLRWLNGGWCLFMLNPWLLGGMGLTAAILIGVLA
                     LIPLLGGLLIALLAPIGLSSTYLAIDGVHKQPMVLPASLRAPALKQSPWQLIAVFRHD
                     AHVLPTAAACLYSAAAVLLTNLPVQVLAGNAWVADWSSLNHASQAGVLMAMALVFALY
                     AVLAASLIYALPLTFLRDEPLIPSIRQSLRASRHFAIALLVLLGALLAPLLLGAITSR
                     LSPWLGYLVWIITGSVILPVITASLYCSYLDIFAIRHHQTGTQMRRATDRQITGADAV
                     TR"
     gene            complement(5138..5521)
                     /locus_tag="AC16FV_07745"
     CDS             complement(5138..5521)
                     /locus_tag="AC16FV_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR2849983.1"
                     /translation="MIGFMLRLIVVAFGLWLAAELVPGIEVHGLWTLLGAALLLGVVN
                     AVVRPLLVILTLPITLLTLGLFLLVVNAAMLGLVAWMFDNFTIAGFWPAIFGSIVVSL
                     VGWFASYFIGPRGRVEVIVLRHRRQ"
     gene            5588..5908
                     /locus_tag="AC16FV_07750"
     CDS             5588..5908
                     /locus_tag="AC16FV_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR2849984.1"
                     /translation="MTWSKAGPLAPAQTWFVPDTGRGTMHGEHHHDLSLEFPELKQRI
                     HDLKLTSPEFNQLYAEYQALDKSIYRIEEEIETPSDSYTEELKKKRVRLKDKLYAMLA
                     TVGH"
     gene            5948..>6276
                     /locus_tag="AC16FV_07755"
     CDS             5948..>6276
                     /locus_tag="AC16FV_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2849985.1"
                     /translation="MYNRVLVPIDGSETSKRALHEAIRIGQCQKAAEIRLLYVIDTIS
                     YIPQTETPPSYIKEIYEQARKYGQDMLEQAKKEIERAGLRVETRLVELKRASDRIAQV
                     IADEAKA"
BASE COUNT         1285 a         1956 c         1880 g         1155 t
ORIGIN      
        1 agataatgaa ggttttgcgt gtcatcgttc atgcatctta acggactggc gcgaacgaag
       61 cacccgcggg cggaacgccg gcggcgttta ctctagcggt tgcgcgcgat ttcgtagccg
      121 atgacggtgc cgatcacggc gccggctgcg gtgtgcccgg gatcgccgta accgatctcg
      181 tgccccacca ccccgccaat cacgctgccg agaataatgg gcgcggacga actcgtgcgg
      241 tgtgccggat agtaaccatg gtcatgacga taatgatgcc gcggcgccgg tctgtaataa
      301 tggtgaggct tgtaccatcc acgatgctgt cccggatggt aataatgccg ctccacgtac
      361 ttctcgcgat acaccggagc cggacgataa tgatcgcggc cgtcccaccc gtcgcggccg
      421 tcacgcgcct gcgcgagtgg agcggccatg acaacggcca gaaataaaac agcggtactc
      481 aggattttgc ggttcatcgt tgcctccttc ataacgtttc aaggtttact cctgacttcc
      541 gtaaatcagg atacaaacct cacgctgaac ggagcctgaa cggatactcg cgttaactga
      601 ttgatatgaa taatctcctg ctctgaaatg gggttcagcc ccggacgtaa cggcaataag
      661 tctcgcgcac gcgcaggctg ccggcaaaac cggccacggc gcaggcaaag aaaatctcca
      721 tgccaggccg atattgcgcc gcggcacccg cggcctgtcc ggcgcggtcg atggtccagc
      781 ctaccagcgg ttgcagaatc ccggtgccga gaaacacgcc ggtattgacg acgctggtcg
      841 ccatgccgga cagcgcatgc gggttcactt ccttcacgca ggcccagctc agggtaaagc
      901 tccccgcgct caggcccatg agcaggagca gtgagtaact cgtagcgagc gacagcggca
      961 acgccaacac cagcggcagc cagcacagca gatgcgccag cgccgtggtg atgagcaccg
     1021 ggcgccgacg cccgaggcga tcggacacaa ggcccaccaa aaaagcaccg actgcaaaac
     1081 ccgcgagcat caaactggtg tgctgcgtcg caaccacgcg ttccatgcca tatacgcccc
     1141 gtagaaacgg taccgcccac aggccggcga aggctatgaa cgacccgcca atgccgagat
     1201 tcatcccgaa gatcggccac gtgtagcggt tcctgagcac cgtcatgagc ccgtcgaacc
     1261 agtgaccctc gtgcggcggg tgggccgcgt ggccgtccat ttcacgcaaa ctcggcagac
     1321 ccgcctcgcc cggatgatcg cgtacaagca gccaggtaaa cacggcgagc acgaatgaca
     1381 cgccgcccaa aacgatgaac acggcgcgcc aggtcgtgaa ctgcaggacc caggcaagcg
     1441 gcgcggccgc cagcaccgaa ccgacgttgc ccaggaccag tgtcaggccg accgcggtgc
     1501 cgaaatggcg gtcgtgaaac cagaccgcgt tgagcttgag gagcgctatg aaggccaccg
     1561 acacaccgag tccgatgagc atgcggccaa tggaagcctc ggttatcgtc gtggcatatc
     1621 cgaacagcag ggaaccgaca gcggcgatga caccgccgag cgtaacgata cgccgcggcc
     1681 cgagggtgtc ggccagaacc ccggctggaa tttgcattac ggtgtacacg tagaaatatg
     1741 ttgcggccag tccgcccaac gccgtggcac tggcattgaa tgcctgctgc agatcggaag
     1801 cgatggcggc tggcgccatc cggtggaaga acgccagcat gtaggacatg gccacgagag
     1861 caaacgcggt ccagcgcagc cggcgcaagc gggcatgcac atcggcaggg gaggcggttt
     1921 tcacgcgtgg tcgattcatg catgatccgg gaatgaagcg gtgattctag caagaatccg
     1981 tttttggctt ggagcaccgt cgccgtacga tacctgtgcc ccggaacctc gccatcgact
     2041 gacctctatt tcaacctcaa acgaaacaac ccgtgagtct cgctgtcgct ggtgaaatag
     2101 agcgcgccat caggaccgat ggccacgccg accggccggg cccaacgctc gccgtccctc
     2161 aactgaaaac ccgtgacgag atcgtccacg cgcatggcct cgccatccct gaatcgcacg
     2221 gcaacaagct tgggtggacg acgcgtggcg ctgtcaccga gataaccgcc gctgggctgg
     2281 gtgccccagg aaccgcgcag cgcgacaatg gcatcgaact caagcgcctt gttcaacgca
     2341 cccttgggca cgaaggcaac gcccatcggc gcgttgcgcg ccggaaaagt tgcgactggc
     2401 ggcacgatat ccttcagtgg ccgcggcggt tggctcgaca cgcagtcatc gcgcctcaac
     2461 tgttttccgt cgaactgaaa ccacggcatg ccgtgaaacg agccctcggt gacacggctg
     2521 aaatattccg gtggctgttc ataaccgagg tggtcggggc cgttgttgct ggcgtacatg
     2581 actttggtta caggatgcca ggcaaaaccc acgggattgc gcagcccgga gccgtatgcc
     2641 cgccagtatg ccttgccgcc ctcctcgcgc aacacaaaaa caccgccacg gcgctcatcg
     2701 aagggataac tgtcgccgag atattgatcg ctgcaattgc cctgaatgcc caggctcacg
     2761 tagacgcgac catcgggacc cacgccgacc gtgcggctgt tatgaccccc gccgccgggc
     2821 aggcgcgcca gcaacttcac cgattccggc gcgatttgct tctgaccggg taggtaggac
     2881 gcacggtata caccattggt acgagcgatg agaatctcac ctttgcgaaa cgcgacactg
     2941 tgcgggtaat catcgagcct gaccagcacc ccgggcgcgg tgtagggcgg gggcaggcgg
     3001 tagacccggc cggatttgga accgatgaag agatcgccgc tatccgcgaa cgtcggcagg
     3061 cgcgggccat cgagtcggtc ggtcagaagc tccagccgga atccttttgg cacgcgcacg
     3121 agctggcgct tgccatcgac catcagcgcc tgttcttcgt aggtcagcgg ctccggcgcg
     3181 gcccacccct ccgccacgac aaggcagagg aaaaacaaaa acgccaatgg gcgaatggcg
     3241 attcgcaaac gcgatatgct ccttcaatat ttgatttact gtgtcttgcc ggtggtgtcc
     3301 tgcggagcgg gcgcggccgg caccgtgggt gcggcttccg tctcggccgc cggtttcagc
     3361 acgatctcga cacggcggtt gcgctgcttg ttctcgtccg tgtcattggg cgccaccggg
     3421 tggtacatcg aaaaacccag cgattcgagg cgcttcggat tgatatgatg cttggtctcg
     3481 atgtagcgcg ccacggccga tgcccgcgcg gcggacagtt cccagttcga gggatatttt
     3541 ttcttgagct tgtcgctgat gggggtgtcg tccgtgtggc cctcgacacg aatggtgtcg
     3601 gtgctgtcgg cgatggcctc cacgagcttg tcgagcaccg ccatgccctc cggcaggatg
     3661 tcggcgctgc cggagcggaa cacgatcttg tccagcatcc gcaccgtgag ctggccgcgc
     3721 agctgctcga tctcgacgct cttgtccgtc agttccttct gcatgcgctc gcgcagctga
     3781 ttttcctgct gctgcacggc ctcgagtttt tgcttgttgg cctcggccgc cttgcgatag
     3841 acatcaagct cctgcgtcgc cgccgtgagt ttggtttcgg tttcgccgcg ggcggtattg
     3901 caggcggcga gttcattctt cgcgcgggtc tcgcagcccg ccagttcggc tgacttctgt
     3961 tcgaaagtcg aggtcgacac acaaccggtc agaaatacgc cgactgccat cactgataaa
     4021 tatattggac gtttcattat tcacctctgc tcaagcattt gttatcgggt ctgtttcttc
     4081 cccatccctt tctttccgtc gcgattttta atgacttcct gcggccggct gttgcagcga
     4141 tggtaacagg tgaccgccat gcggataagt ggggatgggc gccgtttatc cggatgaacg
     4201 gcgcgataac gtcggtcaac gcagtacgcg ggcatggccg gagcagcatg cgcctgtcat
     4261 acagaaattc ctgtgctagc tttattcagt acaaccgggg cggcgggaga ctgcccgagg
     4321 agaacgggct tgcaacggat atcgaaacat ctgcgaatcg ggctgcgctg gttaaacggc
     4381 ggctggtgcc tgttcatgct caacccgtgg ttgctcggtg gaatgggtct cacggcggcg
     4441 attctcatcg gcgtgcttgc gctgatcccg ctgcttggtg gtctgctcat cgcgctgctc
     4501 gcgccgatag gactttccag cacctacctc gccatcgatg gtgtgcacaa gcaaccgatg
     4561 gttttgccgg cctcattgcg ggcgcccgca ttgaaacaat cgccgtggca actcatcgcc
     4621 gtattccgcc atgatgcgca tgtattgcct acggcggcgg cgtgcctgta cagcgccgcg
     4681 gcggtgctgc tgaccaacct gccggtgcag gtcctggcgg gcaacgcctg ggtggctgac
     4741 tggtccagcc tcaatcacgc ctcgcaggcg ggcgtcctca tggcgatggc gctcgttttc
     4801 gcgctctatg ccgtgctcgc ggcgtcgctg atctacgcgt tgccgctcac gtttctgcgg
     4861 gacgagccgc tgatcccttc gatccggcaa agtctccggg ccagccggca tttcgcgatc
     4921 gccctgctgg tgctgctggg cgcgctgctc gcgccgctcc ttctcggcgc gatcacctcc
     4981 cggttgtccc cgtggctggg ttatctggtg tggatcatca ccggctccgt catactgccg
     5041 gtaataaccg ccagcctgta ttgcagctat ctcgacatct ttgccatcag acatcatcag
     5101 acgggaactc aaatgcgccg ggcgactgat cgtcagatta ctggcgccga tgccgtaaca
     5161 cgatgacctc gacacgcccg cgcgggccga tgaaataaga ggcgaaccag ccgaccaggc
     5221 tcacgacaat cgatccgaat attgccggcc agaagccggc gatcgtgaag ttgtcgaaca
     5281 tccacgcaac cagccccagc atggcggcgt tgacaaccag caggaacaat ccgagcgtca
     5341 gcagcgtgat ggggagcgtc aggatcacca gtagcggccg caccacggca ttgacaaccc
     5401 cgagcaacaa cgccgccccg agcagggtcc acaggccatg cacttctatg ccgggcacca
     5461 gttccgcggc cagccataaa ccgaatgcca cgacgatcag gcgcaacatg aaaccgatca
     5521 tgaacttata tcctcactac agggaatcga cgcttggcag tatggcacgg ccgttccacc
     5581 ccatttcttg acctggagca aagccgggcc tctggcgccg gcgcagactt ggtttgtccc
     5641 tgacaccggc cgaggtacca tgcacggcga gcatcatcac gatctgtcac tcgagttccc
     5701 cgagctcaag cagcgcattc acgacctcaa gctgacgagc ccggagttca accagctgta
     5761 tgccgagtac caggcgctgg acaagtccat ctatcgcatt gaagaagaga tcgagactcc
     5821 gtcggacagc tataccgaag aactcaagaa gaagcgcgtg cggctcaagg acaaactcta
     5881 cgccatgctc gcgacagtcg ggcattagcg gccgaagcca gggcgccatc tataaggaga
     5941 cagcatcatg tacaaccgtg ttttggttcc gatcgatggc agcgaaacct cgaagcgcgc
     6001 cttgcacgag gcaatccgga tcggacaatg ccagaaggct gccgaaatcc gcctgttata
     6061 tgtcatcgac accatcagct acataccgca gaccgagact ccgccgtcat acatcaagga
     6121 gatatacgag caggcacgga agtacggaca ggacatgctg gagcaggcga agaaggagat
     6181 tgagcgcgcg gggctgcgtg tcgagacacg gctggtcgaa cttaagcgcg ccagcgatcg
     6241 catcgcgcag gtgatcgcgg acgaggccaa ggccat
//
LOCUS       DBNGZE010000299         3317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1848693, whole genome shotgun sequence.
ACCESSION   DBNGZE010000299 DBNGZE010000000
VERSION     DBNGZE010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1848693"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1158)
                     /gene="corA"
                     /locus_tag="AC16FV_07760"
     CDS             complement(94..1158)
                     /gene="corA"
                     /locus_tag="AC16FV_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR2849986.1"
                     /translation="MTQLVQKRSRKTGLPPGTAVHIGEKKSERTRIQLLHYDAAQMTE
                     REVEGIEDGMSLRPGAGVTWVHVTGVHDVALFDKLGRAFGLHPLVVEDIANTDQRPKL
                     EDYGDYAYVVLKTMQDGSTGQETRIEQLSLILGADFVLSFEESEPTAFEAVRARIRDN
                     RAGIRARGADYLAYSLLDSVIDNYFLAIEGFGNRVEALQQDLIGRSSRRTEGTLHELR
                     REMVLLRKSVWPLREVIGTLERGGSRLFRQETWVYLRDVYDHVIHMVDTLETMHEMLT
                     SMLDLYLLNATNRLNEVIKVLTIIATIFSPPMLIASIYGMNFRFMPELGWPWGYGLAL
                     GLMTVSTVGMLLFFRRKKWI"
     gene            1270..2385
                     /locus_tag="AC16FV_07765"
     CDS             1270..2385
                     /locus_tag="AC16FV_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR2849987.1"
                     /translation="MDMFRVAHATAPDWRQAANACLAQIGDVPAEASLGFLYVTDLLS
                     GDLPNILNFFKEQTDIPHWVGSVGVGVCATSQEYNDQPALSVMIADFPVDSFRVFPAI
                     KSDIEEFSGHRDWCEKADAHFGVVHGDPRNPKTPAFIAELAEAINDGYLVGGLSSSRG
                     PNFQIAGGLTEGGLSGVLFSSRVPVVTAHTQGCSPLGQKHQVTECQNNIAIRIDDRPA
                     LDVLNEEIGEVLARDLSRAAGYIFAGFPIKGSDTGDYVVRNLMGVDTENKLLAVGGYL
                     NPGDTIMFCKRDIRTAYEDLLRMLRDIKSRLNGRPRGGVYYSCLGRGVHMFGEDSAEL
                     RAIRQELGDIPLVGFFANGEISHNRLYGFTGVLTLFS"
     gene            2397..2819
                     /locus_tag="AC16FV_07770"
     CDS             2397..2819
                     /locus_tag="AC16FV_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HIR2849988.1"
                     /translation="MAMVHVPTLFFFDPQAEEGKKQTVKTRATTKSKKEKRLFCAACR
                     HPVTHQDERIPVQGSHEHRCTNPHGITFHIGCFREAAGCSAIGAATTEYTWFPGYAWR
                     IALCANCHAHLGWRFQSPGDYFHGLIVARLTSAGAVKN"
     gene            complement(2825..>3317)
                     /locus_tag="AC16FV_07775"
     CDS             complement(2825..>3317)
                     /locus_tag="AC16FV_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR2849989.1"
                     /translation="FDHELGKEARFVEQEPYLAQEGLLEALHREGLAREACYRIVKLM
                     HEQKSVFVATFQEKVLGLGSADVNNPVKGALVMALSFILGGVLPLAPFAFMPLQWALV
                     AAVLITGAVLFSVGMFKGRLAGQSMGRSGLEFFAIALIATGLGYAVGFLLERYAGAPL
                     PVG"
BASE COUNT          649 a         1063 c          972 g          633 t
ORIGIN      
        1 gcatgctcat attcatcatt tgaggctgtt ggcagctcag gaaatcagca gcgcccataa
       61 tttgagaatt tccctgcgca cccactcagc ttctcaaatc cacttcttgc gcctgaaaaa
      121 gagcagcatc cccacggtgc tgacggtcat cagcccgagc gccaggccat agccccacgg
      181 ccagccgagt tcgggcatga agcggaagtt catgccgtag atgctggcga tcagcatcgg
      241 cgggctgaag atcgtggcga tgatggtgag caccttgatg acctcgttca gccggttggt
      301 agcgttgagc agatagagat cgagcatgga ggtgagcatc tcatgcatgg tttcgagcgt
      361 atccaccatg tggatgacgt ggtcgtagac gtcgcgcaga tacacccaag tctcctggcg
      421 gaacagccgc gagccgccac gctcgagcgt gccgatcacc tcgcgcagcg gccacacact
      481 cttgcgcagc agcaccattt cgcgccgtag ctcatgcagg gtgccctcgg tgcgccgcga
      541 ggaacggccg atgagatcct gctgcaaggc ctcgacacgg ttgccgaagc cctcgatcgc
      601 cagaaaatag ttgtcgatca ccgaatccag aaggctatag gcaagatagt ccgcgccacg
      661 cgcgcggata ccggcgcggt tgtctcggat ccgcgcgcgc accgcctcga aggcagtcgg
      721 ctcggactcc tcgaacgaca gcacgaaatc ggcgccgagg atcaggctca gctgctcgat
      781 gcgcgtctcc tgtccggtgc tgccgtcctg catggtcttg agcacgacat aggcgtagtc
      841 gccatagtcc tcaagcttgg ggcgctggtc ggtgttggcg atgtcttcca ccaccagtgg
      901 gtgcagcccg aaggcgcgac cgagcttgtc gaacaatgcc acatcgtgca cgcccgtgac
      961 atgcacccaa gtcacgcccg cccccgggcg cagactcatg ccatcctcga tgccttccac
     1021 ctcccgttcc gtcatttgcg cggcatcgta atgcagcagc tggatgcgcg tgcgttcgct
     1081 ttttttctcg ccaatgtgca ccgcggtgcc gggcggcagg ccggtcttgc gtgagcgttt
     1141 ttgcacgagc tgggtcataa attcaccttt ttatttcggg gtttgaggaa gcgggttcta
     1201 acccaatcca attatctata gtatcgctcc catcgggctc aagcccatcc tcttatcaag
     1261 ggatttgata tggacatgtt ccgtgtggcg catgccaccg ccccggactg gcggcaggcc
     1321 gccaatgcct gcctggccca gatcggcgat gttcccgcgg aggccagcct gggttttctg
     1381 tatgtcaccg atctgctgtc cggcgacctg ccgaacatcc tcaacttctt caaggaacag
     1441 accgacatcc cgcactgggt cggcagcgtg ggcgtcgggg tgtgcgccac ctcgcaggaa
     1501 tacaacgacc agcccgcgct cagcgtgatg atcgccgatt ttccggtgga cagcttccgc
     1561 gtgttccccg cgatcaagtc cgatatcgag gaattctccg gtcaccgcga ctggtgcgag
     1621 aaagcggacg cgcatttcgg cgtggtgcac ggcgacccgc gcaacccgaa aacgcccgcc
     1681 ttcattgccg aactcgccga ggccatcaac gacggctatc tggtgggcgg gctgagctcc
     1741 tcgcgcgggc cgaatttcca gatcgccggg ggactgaccg agggcggatt gtccggcgtg
     1801 ctgttttcct cccgtgtccc cgtcgtcacc gcccacaccc agggttgttc gccgctggga
     1861 cagaaacacc aggtcaccga atgccagaac aacatcgcca tccgcatcga cgaccgtccg
     1921 gcgctcgacg tactcaacga ggagatcggc gaggtgctgg cgcgcgacct gtcacgcgcc
     1981 gctggctata tcttcgcggg ctttcccatc aagggatcgg ataccggcga ttacgtggtg
     2041 cgcaacctga tgggcgtgga cacggagaat aaattgctgg ccgtgggcgg ctacctgaat
     2101 ccgggcgaca ccatcatgtt ctgcaagcgc gacatccgca ccgcctacga agacctgctg
     2161 cgcatgttgc gcgatatcaa atcgcgcctc aacggccgtc cccggggcgg cgtgtactat
     2221 tcctgcctcg gtcgcggcgt gcacatgttc ggggaagact cggcggaact gagagcgatt
     2281 cgccaagagc tgggcgatat cccgctcgtg ggctttttcg ctaacggcga gatctcgcac
     2341 aaccggctgt acggcttcac cggagtatta acgctgtttt cctgagtgaa acggccatgg
     2401 cgatggtgca tgtccctacc ctgttctttt tcgatcccca ggctgaagaa ggcaaaaaac
     2461 agacggtcaa aacacgcgcg acaacgaaat caaaaaaaga aaagcgccta ttctgcgccg
     2521 cctgtcgcca tcccgtcacg catcaggacg aacgcattcc cgtgcagggc agccacgagc
     2581 accgctgcac caatccccac ggcatcacct ttcatattgg ctgttttcgc gaggccgctg
     2641 gttgtagcgc catcggcgct gcgacgacgg agtacacctg gtttccggga tatgcctggc
     2701 gcatcgcctt gtgcgcgaac tgtcatgcgc acctcggctg gcgcttccag tcaccgggag
     2761 attatttcca cggattgatc gtggcccggc tgacctccgc cggtgcggtt aaaaactgag
     2821 gaactcatcc cacgggcagc ggcgcacccg cgtagcgttc gagcaggaag cccacggcgt
     2881 agccgaggcc agtggcgatc aacgcgatgg cgaagaactc cagccccgaa cgccccatgg
     2941 attggccggc aagccggcct ttgaacatgc cgacactgaa aagcacggcg ccggtgatca
     3001 acaccgccgc caccaaggcc cactgcaaag gcatgaaggc gaatggcgcg agcggcagca
     3061 caccgccgag gatgaaggag agcgccatca ccagtgcgcc cttgaccgga ttgttgacat
     3121 cggcgctgcc cagcccgagc accttttcct gaaacgtcgc cacgaacacg gacttctgtt
     3181 cgtgcatcaa cttgacgatg cgatagcagg cctcgcgtgc cagaccctcg cggtgcagcg
     3241 cctccagcag tccttcctgc gccagatacg gctcctgctc gacgaacctc gcttctttcc
     3301 cgagttcgtg gtcgaag
//
LOCUS       DBNGZE010000300         3946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1776081, whole genome shotgun sequence.
ACCESSION   DBNGZE010000300 DBNGZE010000000
VERSION     DBNGZE010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1776081"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="AC16FV_07780"
     CDS             complement(<1..778)
                     /locus_tag="AC16FV_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR2849990.1"
                     /translation="MPLLRLDKVSLAYGHRPLLERAQLEIRRGERVCLVGRNGEGKSS
                     LIRLLSGEATPDDGNIWIRPGTRIAHLAQEVSPDSDETVFDIVAGGLAGLGRLISQYH
                     HAAMELAHAPTPALTQRLAQLQHELEAGDGWRLEQRVEMVLSRLNLDGDVSMHALSGG
                     WRRRVMLARALVCEPDLLLLDEPTNHLDIEAITWLEEFLTDFTGALLFVSHDRAFLKR
                     LATRIIELDRGALTSWPGSYDDYVRRKQDQLAVEAQHNALF"
     gene            complement(802..1263)
                     /locus_tag="AC16FV_07785"
     CDS             complement(802..1263)
                     /locus_tag="AC16FV_07785"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR2849991.1"
                     /translation="MLQNLKQFFDRHILPAGREEGGNADHALQIATAALLFEVMRTDS
                     EIKDVERHAMTAAIQESFELTAGETASLTRLAEAEAEQATDYYQFTALINKHYTPEQK
                     EQVIEYMWQVASADHEIDRFERTLVHKIADLLHVPRPAQIAARERALRSAT"
     gene            1427..1660
                     /locus_tag="AC16FV_07790"
     CDS             1427..1660
                     /locus_tag="AC16FV_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2849992.1"
                     /translation="MDIPISDVMIHIDETLPREMLTKIEDAVREDECVISASVPADKQ
                     HMMLVAYNPDCTTATDILTRVNKQGVHAELVGF"
     gene            complement(1692..2036)
                     /locus_tag="AC16FV_07795"
     CDS             complement(1692..2036)
                     /locus_tag="AC16FV_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR2849993.1"
                     /translation="MSIEKYQFGHITIDGREYDHDVIVFPRRVRADWWRKHGHRLDVE
                     DLVEVFREPPQVLVIGTGYFGNMAVPQATLAELHERGIETRVLRTGEAVAEINRLQRE
                     LARVVGALHLTC"
     gene            complement(2087..2557)
                     /locus_tag="AC16FV_07800"
     CDS             complement(2087..2557)
                     /locus_tag="AC16FV_07800"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR2849994.1"
                     /translation="MAIATTLENFLKEHHVAYDVVTHPHTMTSMQTAAEAHVPGDRLI
                     KSVVVEDDDGYLMVALPSTHRLELGRLHRQLQRQLGLATENELAGLFKDCELGAIPPI
                     GAAYGIETLVDDSLLQGGDVYFEAGDHEELIHISGKQFQDLFAGAKHGRYSDRV"
     gene            2780..3196
                     /locus_tag="AC16FV_07805"
     CDS             2780..3196
                     /locus_tag="AC16FV_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR2849995.1"
                     /translation="MLVKEIMSHKMESILPTASLREAAKKMRDLDLGSLPVLEDGKLL
                     GMITDRDICCRGIAAGFDPVRTEVREIMSHDVWFCFNDDSVTAAARQMELHHIRRLAV
                     LNRDKTMAGFLSVDDLAHYSRQLAGEVLDTVRPAQH"
     gene            3298..>3946
                     /locus_tag="AC16FV_07810"
     CDS             3298..>3946
                     /locus_tag="AC16FV_07810"
                     /inference="COORDINATES: protein motif:HMM:NF042645.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HIR2849996.1"
                     /translation="MRKQFLALVVAILVLGGCSNTVPTAGADNMSVDFRVFYSELAPY
                     GHWINRLEFGWVWVPHDVPVSWRPYTYGHWIYTDDYGWYWVSYWPWGWAPFHYGRWVY
                     DDEYGWAWAPDTVWGPAWVDWRYGDGWIGWAPLPPIAVWRPRIGFSITIVEIERHIRP
                     TSWVFCRERNFRREHVYRHIELPARNMTIIKSTVNVTRYHLEQDRIVNHGYDVDKL"
BASE COUNT          756 a         1210 c         1200 g          780 t
ORIGIN      
        1 cgaacagcgc gttgtgctgc gcttctaccg cgagctggtc ttgcttgcgg cgcacgtaat
       61 catcgtaact tccgggccag gacgtgagcg caccgcggtc gagctcgatg atgcgcgtgg
      121 cgaggcgctt caaaaacgcg cgatcatggc tgacgaacag taacgcgcca gtaaagtcgg
      181 tgaggaattc ctccagccaa gtgatggcct cgatgtccag atgattggtc ggttcatcga
      241 gcagcagcag gtccggctcg cacaccagcg cacgcgccag catcacgcgc cggcgccagc
      301 cgccggaaag cgcatgcatg ctcacatctc catccagatt cagacgggat agcaccattt
      361 ccacccgttg ctccagccgc cagccatcgc cggcttcgag ttcgtgttgc aactgcgcca
      421 gccgttgcgt cagcgctggt gtcggcgcgt gcgcgagttc catggcggcg tgatgatatt
      481 gagagatcaa ccgaccgagt ccggccagcc cgccggcgac gatgtcgaac acggtctcgt
      541 cgctgtcggg gctgacttcc tgcgccaagt gcgcgatgcg cgtgccgggg cggatccaga
      601 tgttgccgtc atcgggcgtg gcctcgccac tcaagaggcg gatcagcgac gacttgccct
      661 cgccgttgcg tccgacgagg catacccgct cgccgcggcg gatttcgagc tgggcgcgct
      721 ccagtaacgg gcgatggcca taagccaggg aaaccttatc aaggcgcagc aggggcatga
      781 atatcgtaat cagtgacgat gttaagtagc ggagcgcagg gcgcgctcgc gcgcggcaat
      841 ctgcgccggg cgcgggacat gcagcagatc ggcgatcttg tgcaccaggg tgcgctcgaa
      901 gcgatcaatc tcgtgatcgg ccgaggctac ctgccacatg tattcaatca cctgttcctt
      961 ctgttccggg gtgtagtgct tgttgatgag tgcagtgaat tggtaataat cggtggcctg
     1021 ttccgcctcg gcctccgcga ggcgcgtgag cgacgctgtt tccccggccg tcagctcgaa
     1081 actctcctgg atcgcggccg tcatggcatg gcgctcgacg tccttgatct cgctgtcggt
     1141 gcgcatgacc tcgaacagca gtgcggccgt ggctatctgc aaggcatggt ctgcgttccc
     1201 gccctcttcc cgcccggccg ggaggatgtg ccggtcgaaa aattgtttca ggttctgcaa
     1261 catggcgccg gcgattttac cgcatcgcga cggcccaagt ccccggaatg cttgaaataa
     1321 cgggcctcac ggaatcccgc gccacgggaa ataaatacat tctattagtc ttcccacgct
     1381 ggaagaatta ttttgatcac gacgttggac acaggaggtg acaaccatgg atatccccat
     1441 cagtgatgtc atgatccata tcgatgaaac cctgccccgg gagatgctga cgaagatcga
     1501 ggacgctgtg cgcgaggacg agtgcgtgat cagcgccagc gtgcccgccg acaaacagca
     1561 catgatgctg gtggcctaca atccggactg caccacggcc acggacattc tgaccagggt
     1621 caacaagcag ggggttcacg ccgaactggt cggtttttaa ccggacatcc cggcgctctc
     1681 ccccgcgggg ctcagcaggt aagatgcagc gccccgacga cgcgcgccag ttcgcgctgc
     1741 aggcggttga tctcggccac ggcctctccc gtgcgcagca cgcgcgtctc aatgccgcgc
     1801 tcgtgcagct ccgcgagcgt cgcttgcggc accgccatgt tgccgaaata gccggtgccg
     1861 atcaccagca cctgcggcgg ttcccggaac acctccacga ggtcctccac gtccagccgg
     1921 tggccatgct tgcgccacca gtccgcgcgc acgcgccgcg ggaaaacgat gacgtcgtga
     1981 tcgtactccc ggccgtcgat ggtgatgtgt ccgaactggt acttctcaat gctcatcgtt
     2041 cacaccgtta ccgtccggca cgcaggacct tttgcacacg tccggactac acgcgatcgc
     2101 tgtagcgtcc gtgtttcgcg ccggcgaaca ggtcctggaa ctgcttgccg ctgatgtgga
     2161 tcagctcctc gtgatcgccg gcctcgaaat agacgtcgcc gccttgcaac aggctgtcgt
     2221 ccacgagggt ctcgatcccg taggcggcgc cgatcggcgg gatggcgcca agctcacaat
     2281 ccttgaacaa accggcaagc tcgttctcgg tggcaagccc gagctggcgt tgcaactgac
     2341 gatgcaacct ccccagctcc agccgatgcg tggacggcag cgccaccatc aggtagccgt
     2401 catcgtcttc cacgaccacg ctcttgatca ggcgatcgcc ggggacgtgc gcttccgccg
     2461 cggtttgcat gctggtcatg gtgtgtgggt gcgtgacgac atcgtaagca acgtgatgct
     2521 ccttcagaaa attctccaat gtggttgcga ttgccatggc cgtacctcct tgcgaggttc
     2581 atcgccccgc gtaagacagg gttcagagga aacaaacaaa atcatgataa cgccgctcac
     2641 cgatcggggg taccgcttga ccgtcgcggg tcataaaccc gactaatggt ggagtatttt
     2701 gatgcccatc aacgacacaa ccgctcggat tcattgaaat atcgccatgt tgccagctca
     2761 ggcagcaaag gaggtagata tgctggtaaa ggaaatcatg tcgcacaaga tggaatccat
     2821 cctgcctacg gcatccctgc gcgaggccgc gaagaaaatg cgcgatctcg atctcggctc
     2881 actgccggtg ctcgaggatg gcaagctgct gggcatgatc acggatcgcg atatctgttg
     2941 ccggggtatc gccgccggtt tcgatccggt gcgcaccgag gttcgcgaga tcatgtcgca
     3001 tgatgtctgg ttctgcttca acgatgacag cgtgaccgct gccgcgcggc agatggaact
     3061 gcaccacatc cggcggctcg ccgtgttgaa tcgcgacaag accatggcag gctttttgtc
     3121 ggtggacgac ctcgcgcact actcgcgcca gttggccggt gaggtgctgg atacggttcg
     3181 tcccgcgcag cactgaacgc agactcgcca gccaacgcgc cagcgcagta caggcaggtg
     3241 cgcgatcgtg tcccgatttg attctcgaca catcaacagt gtgcgggaag catcgcaatg
     3301 cgcaaacaat ttctggcgtt agtcgttgcc attctcgtcc tcggcggctg ctccaatacc
     3361 gtgcccaccg ccggggccga caacatgagc gtggattttc gggtgttcta cagcgagctc
     3421 gctccctatg gccactggat aaaccggctt gagtttggtt gggtgtgggt gccgcatgat
     3481 gtaccggtgt cctggcgccc gtacacgtat gggcactgga tatacacgga cgattacgga
     3541 tggtactggg tgtcgtattg gccatggggt tgggcgccgt ttcactacgg tcgctgggtt
     3601 tacgatgacg aatacggttg ggcgtgggct cccgacacgg tgtggggacc ggcgtgggtc
     3661 gactggcgct acggcgatgg ctggatcggc tgggcaccgc tgccacccat tgccgtgtgg
     3721 cggccaagga tcggcttcag catcacgatc gtggaaatcg aacgtcatat ccgcccgacc
     3781 agctgggtgt tttgccgcga gcgaaatttc cgacgcgaac acgtgtatcg ccacattgag
     3841 ctgccagccc gcaacatgac aatcatcaag agcaccgtca acgttacccg ctaccacctc
     3901 gagcaagacc gcatcgtcaa ccatggttac gacgtcgaca agctcg
//
LOCUS       DBNGZE010000301         9404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1917966, whole genome shotgun sequence.
ACCESSION   DBNGZE010000301 DBNGZE010000000
VERSION     DBNGZE010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1917966"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16FV_07815"
     CDS             complement(<1..233)
                     /locus_tag="AC16FV_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2849997.1"
                     /translation="MKRTVFLKLATRSLLRNRRRSLITVAAVMIGLAGLVFLWGYIDG
                     INRQMIANITSYLTGHVQVHQKGYHDDPTLDLA"
     gene            complement(230..907)
                     /locus_tag="AC16FV_07820"
     CDS             complement(230..907)
                     /locus_tag="AC16FV_07820"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2849998.1"
                     /translation="MPGVRTEKLTKVYRQDSVAVTALRDVDLEIAAGEFLALVGPSGS
                     GKSTLLNLLGGLDRPTSGRLWVEDAELGALSNRALAELRLKRIGFVFQEYNLIPVLSA
                     LENVEYVMLLQGVPDAERRTRALGILGEVGLSGLEDRRPSELSGGQQQRVAVARAIVS
                     EPALVLADEPTANLDSATGAGLMDLMRQLNEAKGVTFVFATHDTMVMERARRLVRLKD
                     GQIVTEP"
     gene            complement(918..1655)
                     /locus_tag="AC16FV_07825"
     CDS             complement(918..1655)
                     /locus_tag="AC16FV_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR2849999.1"
                     /translation="MFALAVWLVPVAMPAVEPTGRDLVAHVEQLLLGKTNEGLAEMTV
                     VTPRWTRTLELRFWMERPERTFIRILAPPKEAGIGSLRIKAEMWNYLPAVERTIKIPP
                     SMMLQPWMGSDMTNDDLVKESSVVEDYTHRLVGTEDVGGVTAYRVESIPKPEAAVVWG
                     KLVYKIRVADRLPLAQEYYDERGELIKAMRFTEVRNLSGREIPTRWTMQPVKKPQNKT
                     VFLLKEIRFDAPVDPEIFTLRNLQKVK"
     gene            complement(1730..2470)
                     /locus_tag="AC16FV_07830"
     CDS             complement(1730..2470)
                     /locus_tag="AC16FV_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG cluster 4 domain-containing protein"
                     /protein_id="HIR2850000.1"
                     /translation="MMRDPVCLMSIDPRTAVAMVEHGGRTYYFCSTGCRDKFLAQPAL
                     FLDKAPGMRLTVGVMGSADADHPETVRALARDLGRAIAARGCILVTGACPGLPYECAR
                     GTRAAGGLSVGISPALSLDEHVHKYLSPSDVYDVLIYTGSGLMGREVTNIRSSDMVVI
                     VGGRSGTLGEFAIAYDEGKLIGVLQGTGGIVERLPEVVASFGAKETGARLVYDADPVA
                     LIRRLAEMYTTHHYRKPSCFCDERAAAG"
     gene            complement(2467..3081)
                     /locus_tag="AC16FV_07835"
     CDS             complement(2467..3081)
                     /locus_tag="AC16FV_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850001.1"
                     /translation="MDNAVALVRAYLHVNGYFTVTEYPVVEAMRSGDYRMATDLDVLA
                     FRFPGAGCLVPGRHGRHEQVLETDSALGTSREQADMLIGEVKEGRAELNRGARNPEVL
                     RAVLTRFGCCAPEQVPAVVERLLRHGQGLTASGHRVRLVAFWTTTDDPGSGYETIALG
                     QVIQFLQEYLHQHWPVLRHAQFKDPALGFLMTLKKSQRAEKEVP"
     gene            complement(3169..3615)
                     /locus_tag="AC16FV_07840"
     CDS             complement(3169..3615)
                     /locus_tag="AC16FV_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2850002.1"
                     /translation="MDPKLLAVSLLILRIALAVFLLLFGIDKLVATDTTVEVFAHFYG
                     LSISAPVAQGAGILEILLSLAMLAGLWKTATYGLGLTLHAISTLATYEQLLSPFGENH
                     LFIAGIPALAAFVALFLLRRHDTLWSFGRARKGEPGDARLSKQRGQ"
     gene            complement(3634..4062)
                     /locus_tag="AC16FV_07845"
     CDS             complement(3634..4062)
                     /locus_tag="AC16FV_07845"
                     /inference="COORDINATES: protein motif:HMM:NF036887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AF1514 family protein"
                     /protein_id="HIR2850003.1"
                     /translation="MIKTDTSEKEGVVTCEVCLKEIPRSTARSLEGPDYVYHFCGSRC
                     YEKWLAGAGMREIGLEVSGIELDFEAAQSLAQTAARWYAEDAMMLAWFDRSRGKESPA
                     VPECQHKPGWLAYAESHGGDLKIDVNRGAYVFVFASRKQD"
     gene            complement(4172..4432)
                     /locus_tag="AC16FV_07850"
     CDS             complement(4172..4432)
                     /locus_tag="AC16FV_07850"
                     /inference="COORDINATES: protein motif:HMM:NF022603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HIR2850004.1"
                     /translation="MTERSTIRTEKVPASERTAPGFATKVTRRASSGARRAPVSAEER
                     ECMIAMAAYYRAERRGFAPGRELEDWYEAAAEIDRRLGSRSA"
     gene            complement(4485..4943)
                     /locus_tag="AC16FV_07855"
     CDS             complement(4485..4943)
                     /locus_tag="AC16FV_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850005.1"
                     /translation="MTTACVTRPGWLLAGLLLVVSATAAESSYRQIVDGVAVYFGMMP
                     AELVRGHPRQHPESEMHGGVPAGENHLTVALFDDKTGGRIADAQVTATITGPERFKLE
                     KKLEPMIVAGAATYGNYFNMPGPGPYRIALRIRVPGVGHDIQAVFTWARS"
     gene            complement(4999..5484)
                     /locus_tag="AC16FV_07860"
     CDS             complement(4999..5484)
                     /locus_tag="AC16FV_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850006.1"
                     /translation="MKQSMCNRLGAGLLVLGLALALPAYAQRGGGMGGGGTMGGQKGG
                     MMDGGKGMDSGAQQKGSGKMGGQQGMGGPGAQQMSGTMHDMSSQMMDMSGKMSKGSLS
                     AAEQKQMADRMREMAGMMDHMSGMAGKGMMMDADMQKQMDQMRKRMDEMMKPSAGGSK
                     K"
     gene            complement(5603..6259)
                     /locus_tag="AC16FV_07865"
     CDS             complement(5603..6259)
                     /locus_tag="AC16FV_07865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR2850007.1"
                     /translation="MNPAATAPTYGLWSLVVINSAVFIIFLYSFAKPQTKRDWRSFGA
                     ISAFLVALFTEMYGYPLTIYLLSGWLQSRYPNVNWFSHDAGHLLEMLFGWKTNPHFGP
                     FHLLSFAFIGGGFILLSNAWRVLYAAQKNRMLATTGPYARMRHPQYVGFVLIMLGFLV
                     QWPTILTLAMFPVLVFMYVHLARREEREVEAEFGAEYRRYAARTPAWIPRFGMKRALP
                     "
     gene            complement(6256..6477)
                     /locus_tag="AC16FV_07870"
     CDS             complement(6256..6477)
                     /locus_tag="AC16FV_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HIR2850008.1"
                     /translation="MTHEQHAHDATKRGPRINWVLIGFLAIAAFFLLTEHRAHALGAL
                     PFLLLALCPLLHFFGHGGHDHGAEKGDKP"
     gene            complement(6497..6748)
                     /locus_tag="AC16FV_07875"
     CDS             complement(6497..6748)
                     /locus_tag="AC16FV_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850009.1"
                     /translation="MSSAKTFRNRPRIGLCALAMALLAAPILLWWGVTPLTAVLIAIF
                     VSCLVAALYAWWLSWRVLRPLDTVKMPPAVDEINSGDQP"
     gene            complement(6745..9096)
                     /locus_tag="AC16FV_07880"
     CDS             complement(6745..9096)
                     /locus_tag="AC16FV_07880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR2850010.1"
                     /translation="MATDPVCDMTVDERGAAGSIAHAGVKYYFCSAKCMNEFKANPAA
                     YVHGRVVTMKTAVGQRHVHVMPSTAPSPRATMPSTKELAKDPICGMVVEKATALKTEH
                     AGRVYYFCSTGCQRTFESPEAELKSMKTRVTIALTGVLALAFLRAAAFIFLAAGATLV
                     TWAPIPQLPWFTWGMWLFILVTPVQFIGGWGFYKGTWTAIRTHTINMDTLIALGTTVA
                     YLYSVAVIFFPEVLPVKVEERDVYFEVSAVIIAFVLLGKYMEEMIKKRSSAAVRKLMD
                     LKPATAHVLRDGQEMEVPAESVMVNEILIVKPGEKIPTDGEVIEGASSVDESMLTGES
                     MPVEKKPGMSVIGGTLNKTGLFRFRATRVGAETALAQIIKMVEEAQASSAPIQRLADQ
                     VTRYFVPAVVVTAFVSFGGWWLTGNFPQGLLAFIAVLIIACPCALGIATPAALMVGVG
                     KGAEAGILIRGGEVLERARSLTTVVFDKTGTLTRGEPNVTDIMPFADLGEAEVLRLAA
                     AVEAGSEHPLGEAIVRAAKHREIALPKVSGFEAVPGHGIRGQIEDTAVLLGNRRLFAR
                     EGIEAKAAEETMTRLEQEGKTAMLIGANGKLAGVIAVADTLKPEAKEAIMALQQESIE
                     VVMLSGDNRRTAEAIGRELGIANVIAEVLPGDKAKIIQGLQQQGQVVAMVGDGVNDAP
                     ALAAADIGIAIGSGSDVAKETGGIILVRDDVREVVTSIRLSRATLGTIKQNLFWAFIY
                     NTVGIPVAALGFLNPIIAAAAMALSSLSVIVNSALLKRQRLAA"
     gene            complement(9134..>9404)
                     /locus_tag="AC16FV_07885"
     CDS             complement(9134..>9404)
                     /locus_tag="AC16FV_07885"
                     /inference="COORDINATES: protein motif:HMM:NF039498.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HIR2850011.1"
                     /translation="ERLNPWVMGAALTITFVVVYVLCAAAFAVAPAATIGFFNAWFHG
                     LNLAGLQVGAKPFTLGVFLYGTAGIAAVAFVCGVVFAASYNLLRR"
BASE COUNT         1745 a         3144 c         2823 g         1692 t
ORIGIN      
        1 aacgcgagat cgagcgtggg gtcgtcgtgg tagcccttct ggtgcacctg cacgtggccg
       61 gtgagatagc tcgtgatgtt ggcgatcatc tggcggttga tgccgtcgat gtagccccag
      121 agaaacacga gaccggcgag accgatcatg accgccgcga cggtgatgag actgcggcgc
      181 cggttgcgca gcaggctgcg cgtcgcgagc ttgaggaaga ccgtccgttt catggctcgg
      241 tcacgatttg cccgtccttc aggcgcacca gccggcgcgc acgctccatt accatcgtgt
      301 catgagtcgc aaacacgaag gtgacgccct tcgcctcgtt caattggcgc atgaggtcca
      361 tgagcccggc gccggtggcg gaatcgaggt tcgcggtcgg ctcgtcggcg agcaccagcg
      421 ccggctcgct cacgatcgcg cgcgccaccg ccacgcgctg ttgctgtccg ccggaaagct
      481 cgctgggcct gcggtcttcg agtccggaaa gccccacttc gccgagaatg ccgagcgcgc
      541 gggtacggcg ctcagcatct ggcacgcctt gaagcaacat cacgtattcg acgttctcaa
      601 gcgcggaaag caccgggatc aggttgtact cctggaacac gaaaccgatg cgtttcaggc
      661 gcagctccgc gagcgcgcga ttcgagagcg cgcccagttc tgcgtcttcc acccacagcc
      721 gcccagaggt ggggcggtcg agcccgccca gcagattgag cagcgtggac ttgcccgagc
      781 ccgacggtcc cacgagcgcg aggaactcgc ccgcggcgat ctcaagatcc acgtcccgga
      841 gcgccgtgac cgcgacgctg tcctggcggt agaccttggt gagcttttcg gtgcgaacgc
      901 ctggcatctg tccctcctta cttaaccttc tgcaaattgc gcaacgtgaa gatctctggg
      961 tccaccggcg catcgaagcg gatctccttg agcaggaaca ccgtcttgtt ctgcggtttc
     1021 ttcaccggct gcattgtcca gcgcgtcggg atttcgcgcc cgcttaagtt tcgcacttcg
     1081 gtgaagcgca tcgctttgat aagctcgccg cgctcgtcgt aatactcctg cgccagcggc
     1141 aagcggtcgg cgacacggat cttgtacacg agcttgcccc agaccaccgc ggcctcgggt
     1201 ttgggaatcg actcgacgcg gtaggccgtc acgcccccga cgtcctcggt cccgacgagc
     1261 ctgtgcgtat agtcctcgac cacgctgctt tccttcacca gatcgtcgtt ggtcatgtcc
     1321 gagcccatcc acggctgcag catcatcgac ggcgggatct tgatggtgcg ctcgaccgcg
     1381 ggcaggtaat tccacatctc cgccttgatg cgcagcgagc cgatgccggc ctccttcggc
     1441 ggcgcgagga tgcgaatgaa ggtacgctcg ggccgctcca tccagaagcg cagttcgagc
     1501 gtgcgcgtcc agcgcggcgt caccaccgtc atctccgcaa gcccctcgtt cgtcttgccc
     1561 aacagcagct gctcgacatg cgccaccaga tcccggccgg ttggttcgac ggctggcatc
     1621 gcaacgggaa cgagccagac agccagcgca aacatcgcgg cacgccgggg cctgaccgcc
     1681 ctagagcgcc tgacaagtcg tgcaaacatg cgtcttccct cccgcgcgct catccggccg
     1741 ccgcgcgctc gtcgcagaag cacgatggtt tcctgtagtg atgggtcgta tacatctcgg
     1801 cgagccggcg gatcaacgcc accggatcgg cgtcgtaaac gagacgcgca ccggtctcct
     1861 tggcgccgaa gctcgcgacg acctcgggca atcgctcgac gatcccgccc gtgccctgga
     1921 gcacgccgat cagcttgccc tcgtcgtagg caatggcgaa ttcgccgaga gtgccggagc
     1981 gcccgccaac gatcaccacc atgtcactcg accggatgtt ggtcacttca cgccccatga
     2041 gtccgctgcc ggtatagatc agcacatcgt aaacatcgga gggcgagaga tacttgtgca
     2101 cgtgctcgtc gagcgacagc gccggcgaaa tgccgaccga cagcccgccg gcggcgcgcg
     2161 tcccgcgcgc gcactcataa ggcaggcccg ggcaggctcc ggtaacaagg atacaaccac
     2221 gcgctgcaat cgcccgaccc aggtcgcgcg caagtgcgcg taccgtttcc gggtggtcgg
     2281 cgtcggcgga acccatcacg cccacggtga ggcgcatgcc aggcgccttg tcgagaaaca
     2341 gtgccggctg cgcgagaaac ttgtcgcgac aaccggttga gcagaaataa taggtccggc
     2401 cgccgtgctc gaccatggcc acggcggtac gcgggtcgat agacatgagg cagaccggat
     2461 cgcgcatcat ggcacctcct tttccgcacg ctggcttttt ttcagcgtca tcagaaaacc
     2521 cagcgccggg tccttgaact gcgcgtggcg caacaccggc cagtgttgat gcagatattc
     2581 ctgcaggaac tgaatcacct gtccaagcgc gatcgtttca taaccggaac cgggatcgtc
     2641 agtggtcgtc cagaaggcaa cgaggcgcac acggtgacca ctggcggtca acccctgccc
     2701 gtgacgcagg agccgttcca ccacagccgg gacctgttcc ggggcgcaac agccgaagcg
     2761 cgtcagcacg gcgcgcagga cctcggggtt gcgcgcgccg cggttgagct cggcgcgtcc
     2821 ttccttcacc tcgccgatca gcatgtcggc ctgctctctc gacgtcccga gggcggagtc
     2881 ggtttccagc acctgctcgt ggcggccatg ccggccgggc acgagacaac cggcgccggg
     2941 gaaccggaac gccagcacat cgagatcggt cgccatgcgg tagtcgccgg agcgcatggc
     3001 ctcgaccacc ggatactcgg tcacggtgaa ataaccgttc acgtgcaggt aggcacgcac
     3061 cagcgccacg gcgttatcca tcacggtccc tcctttcgct atctcaatgc ctcttcgttt
     3121 cgcgagcctc gtggcgcgcg gtcgcgttca tggccgccgc gctccgggtt attggccgcg
     3181 ctgtttcgat aggcgtgcat cgcccggctc gcctttccgt gcccgcccga aactccagag
     3241 cgtatcgtga cggcgcagca ggaacagcgc cacgaacgcc gcgagcgccg gtatgccggc
     3301 gataaaaaga tggttctcgc cgaagggaga cagcaactgt tcatacgtcg ccagggtgga
     3361 aatcgcgtgc agcgtcaatc ccaacccata agtcgcggtc ttccacaaac cggccagcat
     3421 ggccaggctc agcagaattt ccagaatccc ggccccttgg gcgacggggg cggaaatcga
     3481 aagaccgtaa aaatgcgcaa acacctccac tgtcgtgtcc gtcgccacca gtttgtcgat
     3541 gccgaagagc agcaggaaca cggccagggc gatgcgcaaa atgagaagcg aaacggcaag
     3601 cagcttgggg tccatggtga cctccttgga aagtcaatcc tgtttccggg atgcgaagac
     3661 aaatacatag gcgccgcgat tgacatctat cttcaagtcg ccgccgtggc tctcggcata
     3721 ggccagccac ccgggtttgt gctggcattc cgggaccgcc ggcgattcct tgccgcggct
     3781 gcgatcaaac caggcgagca tcatggcgtc ttccgcatac caccgtgccg ccgtttgcgc
     3841 caaagactgg gctgcttcga aatcgagctc tatcccgctc acctccagcc cgatttcgcg
     3901 catgcccgcg ccggccaacc atttttcata gcaccgcgaa ccgcagaaat gatagacgta
     3961 gtccgggccc tccagactgc gggccgtcga gcgtggaatc tccttgaggc agacctcaca
     4021 ggtgaccact ccttcttttt cagacgtgtc ggtcttgatc atggcgcacc cctgctttca
     4081 gtcgtttaaa ggttatcgat ttagagcgcc tgacgaaatg agcgtggccg ccacgcccct
     4141 ctcccggaag gagagggggt ttcagcagcc ttcaggcgct ccttgaacca agccgccggt
     4201 cgatctccgc cgcggcctcg taccagtcct ccagctcgcg gcctggagca aagccgcggc
     4261 gctcggcgcg gtaatacgcc gccatggcga tcatgcactc acgttcctcg gcgctgaccg
     4321 gagcgcgacg cgcgccgctg ctggcacgcc gcgtaacctt ggttgcgaaa cccggcgccg
     4381 tccgttccga tgccggaacc ttctctgtgc gaatggtgct gcgttcagtc atgatcaaac
     4441 ctccattatg agaacgattg aatgatccca acgatgcccg cggtttacga ccgcgcccag
     4501 gtaaacaccg cctggatgtc gtgccccaca cccggcaccc ggatacgcag cgcgatgcga
     4561 tacggaccag gaccaggcat gttgaagtaa ttgccgtaag tcgcggcgcc ggcgacgatc
     4621 atcggctcga gttttttctc cagcttgaag cgctccggac cagtgatcgt cgcggtgact
     4681 tgggcatcgg cgatgcgccc gccggtcttg tcatcgaaca gcgcgaccgt gaggtggttc
     4741 tcgccggcgg gcacaccgcc gtgcatctcg ctctcgggat gctggcgtgg atgcccgcgc
     4801 accagctccg ccggcatcat gccgaagtac acggcgacgc cgtccacgat ctggcggtag
     4861 gacgactccg ccgcggtggc ggataccacc agcagcaatc ccgcgagcaa ccacccgggt
     4921 cttgttacgc atgctgtcgt catgacacac ctccacgaga aaccacaatc ggcggtttcc
     4981 gaccgccggc ggcattgcct acttcttcga acctccggcc gatggcttca tcatctcgtc
     5041 catgcgcttg cgcatctgat ccatctgctt ctgcatgtcg gcgtccatca tcatgccctt
     5101 gccagccatg ccggacatat ggtccatcat gcccgccatc tcgcgcatgc gatcggccat
     5161 ctgcttctgc tcggccgcgc taagactgcc cttggacatc tttccggaca tatccatcat
     5221 ctggctggac atgtcgtgca tcgtgccgga catctgctgc gcgcccggcc cgcccatgcc
     5281 ctgctgcccg cccatcttgc cgctgccctt ctgctgtgcg ccggagtcca tgcccttgcc
     5341 accgtccatc atgccgcctt tctgcccacc catcgtgccg cctccaccca tgcccccgcc
     5401 cctctgggca taagcgggca gcgccagcgc aagccccagt accagcaggc ctgctcccaa
     5461 cctgttacac attgactgtt tcatggtttt gctccttggc tgtggggatt gctccccgat
     5521 catcattacg tcccgtgacg ggacgctcct ttttcggatc aagcgactcc gaaatcattg
     5581 ccaccgtacc ggcaccacgg tctcacggca gcgcgcgctt catgccgaag cgcggaatcc
     5641 acgccggcgt gcgcgccgcg tagcggcggt actccgcgcc gaactccgct tccacctcgc
     5701 gctcctcgcg ccgggcaaga tgcacgtaca tgaacaccag caccggaaac atggcgagcg
     5761 tcaggatcgt cggccactgc accaggaagc ccagcatgat gagcacgaag ccgacgtact
     5821 gcggatggcg catgcgcgcg tacgggccgg tcgtcgccag catgcggttc ttctgcgccg
     5881 cgtacagcac gcgccaggca ttcgacagca gaatgaaacc gccgccgatg aacgcgaaac
     5941 tcagcaggtg aaacggccca aaatgcgggt tggtcttcca gccgaacagc atctccagca
     6001 aatgcccggc gtcgtgggag aaccagttga cgttcgggta gcggctctgc agccagcccg
     6061 acaacagata gatggtgagc gggtagccgt acatctcggt gaacagcgcc accaggaacg
     6121 ccgagatggc gccgaaggag cgccagtcgc gcttggtctg cggcttggcg aagctgtaga
     6181 gaaagatgat gaacacagcc gagttgatga ccacgagcga ccacaggccg taggtgggcg
     6241 cagtggcggc ggggttcatg gcttgtctcc tttttccgcg ccgtgatcat gtccgccgtg
     6301 gccgaagaaa tgcagcagcg ggcacaatgc caacagcagg aacggcagcg cgccgagagc
     6361 atgggcgcgg tgctcggtca gcaggaagaa cgcagcgatg gcgaggaagc cgatcagcac
     6421 ccagttgatc cgcggtcccc gcttggttgc gtcatgcgca tgttgttcgt gtgtcatggc
     6481 acgaacctct cggtcttcat ggttggtctc ctgaattaat ttcgtcaacg gcaggcggca
     6541 tcttcaccgt gtcgagcggg cgcagcacgc gccacgaaag ccaccaggca tacaacgccg
     6601 cgacgaggca ggaaacgaag atggcgatga gcacggcagt gagcggcgtt acgccccacc
     6661 acaacaggat gggcgccgcc aatagcgcca ttgccagcgc gcacaggccg atgcgagggc
     6721 ggttcctgaa agttttcgcc gagctcatgc cgccagccgc tggcgcttga gcagcgccga
     6781 gttgacgatc accgagagcg acgacaacgc catcgccgcg gcggcgatga tcgggttcag
     6841 gaatccgagt gctgcaaccg gaatgccgac agtgttgtag atgaaagccc agaacaggtt
     6901 ctgcttgatc gtcccgagcg tcgcgcgcga cagacggatg ctcgtcacca cctcgcgcac
     6961 gtcgtcgcgc accaggatga tgccgccggt ttccttggcg acgtccgaac ccgaaccaat
     7021 ggcgatgccg atgtcggcgg cggccagcgc tggtgcatca ttcacgccgt cacccaccat
     7081 cgccacgact tggccctgtt gctgcaagcc ctgaatgatc ttggccttgt cgcccggcaa
     7141 tacctcggca atcacgttcg cgataccgag ctcgcggccg atggcctcgg cggtgcggcg
     7201 gttatcgccg gagagcatca ccacttcgat gctttcctgt tgcagcgcca tgatcgcttc
     7261 cttcgcttcc ggcttgagcg tgtcggccac ggcgatgacg ccggcgagct tgccgttcgc
     7321 gccgatgagc atcgcggttt ttccttcctg ttcgaggcgc gtcatcgttt cctccgcggc
     7381 tttggcctcg atgccttcgc gcgcgaacag ccggcggtta ccgagcagca ccgcggtatc
     7441 ctcgatctgc ccgcgaattc catggccggg aaccgcctcg aatcccgaaa ccttcggcag
     7501 tgcgatctcg cggtgtttcg ccgcgcgcac gattgcctcg ccgagcgggt gctccgagcc
     7561 cgcctccacc gcagccgcga gtctgagcac ttcggcttcg cccaagtcag cgaacggcat
     7621 gatgtcagtt acattcggct cgccacgcgt gagcgtgcca gtcttgtcga ataccacggt
     7681 agtaaggctg cgcgcgcgct ccaacacctc gccgccgcga atgaggatgc ccgcctccgc
     7741 acccttgccc acaccaacca tgagtgccgc cggggtggcg atgccgagcg cacacgggca
     7801 agcgatgatg agcacagcga tgaaggcgag cagtccttgc gggaagttgc cggtaagcca
     7861 ccaaccgcca aaggaaacga atgccgtcac caccactgcc ggcacgaagt agcgcgtgac
     7921 ctggtcggcg aggcgctgga tgggcgcgct gctcgcctgc gcctcctcga ccatcttgat
     7981 gatctgtgcc aacgcagtct cggcgcccac gcgtgtcgcg cgaaacctga ataggccggt
     8041 cttgttgagc gtgccgccga tgaccgacat gcccggcttc ttctccaccg gcatggactc
     8101 gccggtgagc atggactcgt caacgctcga ggcaccctcg atcacctcgc catcggttgg
     8161 gatcttttcg ccgggcttga cgatcaagat ctcattcacc attaccgatt cggccggcac
     8221 ttccatctcc tggccgtcgc gcagcacatg cgcggtagcg ggtttcaggt ccatgagctt
     8281 gcgcacggcg gcggatgaac gcttcttgat catctcctcc atgtacttgc cgagcagcac
     8341 gaaggcgatg atcacggccg atacttcgaa gtacacgtcg cgctcctcga ccttcaccgg
     8401 cagcacctcg gggaagaaga tcacggcgac actgtagaga tacgccacgg tcgtgccgag
     8461 cgcgatcaac gtgtccatat tgatggtgtg cgtccggatc gcggtccaag tgcccttgta
     8521 gaaaccccat ccgccgatga actgcaccgg cgtcaccaga atgaagagcc acatgcccca
     8581 ggtgaaccat ggaagctgcg ggatcggcgc ccaggtgacg agggtggcgc cggcggcgag
     8641 gaagataaat gccgcggcgc gcagaaacgc cagcgccagc acgccggtga gcgcgatggt
     8701 gacgcgcgtc ttcatgctct ttaactcggc ttccggcgac tcgaaggtac gctggcaacc
     8761 ggtggagcag aagtaataga cgcgcccggc atgttcggtt ttcagcgccg tggccttctc
     8821 caccaccatg ccgcagatcg ggtccttggc aagttccttt gtagaaggca tggtcgccct
     8881 aggcgagggt gcggttgacg gcatcacgtg gacatgacgc tggcccaccg ctgtcttcat
     8941 ggtcacgacc ctgccgtgca cataggccgc cgggttcgcc ttgaactcat tcatacattt
     9001 cgccgaacag aagtaatact tcacgccggc atgagcgatg ctgcccgccg ccccacgttc
     9061 atccaccgtc atgtcacata ctggatcggt tgccatccgt gctccttatt catgccaggc
     9121 ataccgcgcg gcgttagcgc cgcaacaggt tatagcttgc cgcaaacacc acgccgcaga
     9181 cgaacgccac cgcggcgatg cccgccgttc cgtaaagaaa tacacctaac gtgaatggct
     9241 tggcgccgac ttgcaggccg gccagattca ggccgtggaa ccaggcgttg aagaagccga
     9301 ttgtcgcggc cggcgcgaca gcgaacgccg cggcgcaaag cacgtagacc acgacgaagg
     9361 tgatggtgag cgccgcgccc atgacccagg gattaagcct ttcc
//
LOCUS       DBNGZE010000302         3430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1154320, whole genome shotgun sequence.
ACCESSION   DBNGZE010000302 DBNGZE010000000
VERSION     DBNGZE010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1154320"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="AC16FV_07890"
     CDS             <1..160
                     /locus_tag="AC16FV_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR2850012.1"
                     /translation="ELAYDFHTYYGAHTFLVEDAALRDARLNLIVATRQVIANGLKLL
                     GVSAPESM"
     gene            257..826
                     /locus_tag="AC16FV_07895"
     CDS             257..826
                     /locus_tag="AC16FV_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR2850013.1"
                     /translation="MARRRRNFSVRRFRKPGWLFLLVGIGIGVAGVMLWQMYSHRGDG
                     RSGLGNLFSSFSKPAEKPVKKEPEPAKPPKPKFDFYTILPETETVLPERRSKTKAAKA
                     KPEEGVSYILQAGSFAGFEEADQMKARLALAGLVAHIQKITIEGKGEYHRVRLGPYEN
                     IDQLDATAAQLQKMGIKAIRLKVKKGETG"
     gene            complement(837..2728)
                     /gene="speA"
                     /locus_tag="AC16FV_07900"
                     /pseudo
     CDS             complement(837..2728)
                     /gene="speA"
                     /locus_tag="AC16FV_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953937.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
     gene            2826..>3430
                     /gene="speE"
                     /locus_tag="AC16FV_07905"
     CDS             2826..>3430
                     /gene="speE"
                     /locus_tag="AC16FV_07905"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR2850014.1"
                     /translation="MALDKTWYTEEWAGQGSAISLKIKDKVHDEKSPYQRIEIYETET
                     FGTLMTLDGLVMVTDRDNFVYHEMMSHPALFTHPDPKRVLIIGGGDCGTLREVLRHQS
                     VELAEQVELDERVTRVSEKFFPELCESNNDPRAKLHFDDGIKWVADAKPETYDVIIID
                     STDPVGPAAGLFSETFYKNCFRALRPHGVVVGQSESPLFHA"
BASE COUNT          707 a         1024 c         1046 g          653 t
ORIGIN      
        1 cgaactcgcc tatgactttc acacctatta cggcgcccat acctttctgg tcgaggacgc
       61 tgcgctgcgc gacgcccgcc tcaacctgat cgttgccacg cgccaggtga tcgcaaatgg
      121 attgaagctt ctgggtgtgt cagctccgga atcaatgtaa agtaacttgt catatctcag
      181 gacgaacgag cggagcgagt agggctacca gtcatatctc aggacgaacg gagcgaagcg
      241 gagtagggct acatttatgg cgcgcaggcg cagaaatttc tcggtacgcc gttttcgcaa
      301 acccggctgg ttgttcctgc tggtgggcat cggcatcggc gtggccgggg tgatgctgtg
      361 gcaaatgtac tcccaccgtg gcgatggtcg cagcggactg ggaaatctct tttccagttt
      421 cagcaagccg gcggagaagc cggttaaaaa agaaccggag ccggccaaac cccccaagcc
      481 gaaattcgat ttctacacca tcctgccgga aaccgagacc gtgttgccgg agcggcgcag
      541 caagaccaag gccgccaagg ccaaaccgga agagggcgtc agctacattc tgcaagcggg
      601 ttcgttcgcg ggtttcgagg aagccgacca gatgaaggcc aggctcgcgc tggccggtct
      661 ggtggcgcat atccagaaaa tcaccatcga gggcaagggc gagtatcacc gcgtgcgcct
      721 cgggccctac gagaatatcg atcagctcga tgccaccgcc gcgcagttgc agaaaatggg
      781 catcaaagcg attcggctaa aggtcaaaaa gggcgaaacc ggttagctcc gcacttttag
      841 tcttccagat aggtataacc tttcaatccc tcggccagct cggccagata gctttcgcgg
      901 tttttcgcgc tgagggatgg cgcgcgctcg gtcttggcgc ggtaggaagc gagcagatcg
      961 tcggcgttga agtgcacgta gcgcagcacc gagtccacag tatcgccgcg cgcagcttgc
     1021 agcagccgat ggctgccgtc gggcatcagc tcgacgtgga tggaatcggt atcgccgaac
     1081 aggttgtgca tgtcgccgag aatctcctga taggcgccca ccagaaaaat ccccagcaaa
     1141 taaggctcgt gctcgcgcag cggatgcagc ggcaacgtcg agtcgatacc ttcgctgtcc
     1201 acgtacaggt cgaggcgccc gtcggaatcg caggtgatgt cctgcagcac ggcgcggcgc
     1261 gtcggcggct cgttcaggcg attcaacggt accaccggga aaatctggtc gatggcccag
     1321 gcgtcgggca tggactggaa tagcgaaaaa ttgcagaaat atttatcagc gagtttctcg
     1381 ttcagctcgt cgagaatctc gcggtgcgtg cggcttgccg gctgcaacag ctcgcgcacc
     1441 ttccagcaga tggcaaagta aatctgctcg gcgcgcgcgc gctgcgccag cgtcaacacg
     1501 ccgtggctga acatgccctg cgcctcggtc agccggtagg cggcatcgtg gtaggcctcg
     1561 accacggaac gctccgacag cgtcgcgagc gtctgccaca ggtcgcggat gatcgccggt
     1621 tcgctttcag tcgccttcgg caccgaatca gcgcccgggg cggactcggt gtccaccacg
     1681 ttggtgatca gcactgcgtg gtgcgcggtc atggcacggc cggattcggt gacgatgtcg
     1741 gggtggggca gattcttttc cgcgcagatc tcgctcagtg catgcacgat gttgtgcgcg
     1801 tattctgcga ccgagtaatt catggaacag aaactgcgcg agcgcgtgcc ctcatagtcc
     1861 acacccagtc cgccacccac gttgagcacc ttgatatccg cgcccgcggc gcggaattcg
     1921 gcgtagtagc gcgcggcctc gcgcatgccc agctggacat gctggatgtt ggcgatctgc
     1981 gaacccatat ggaagtgcat catttgcagg ctgggcagca gattcgccgt gcgcaggcgc
     2041 gcgatggcgt ccaggacctg agaggccgac agaccgaatt tcgatttctc gccgccggtg
     2101 ttctgccact tgccggcgcc gatcgaggcc aggcgcatgc gcagtccgat cagcggcgta
     2161 ataccaagat ttttggactc ctggatgacc atctcgagct ctgtcggttt ttccacgacg
     2221 atgtacacgc gatgtcccag ttccttgccg atcagcgcca gacgaatgta ctcgcggtcc
     2281 ttgtagccgt tgcacaccac cacaccgccg tcctggcgtg actgcgccaa caccgccagc
     2341 agctcgggct tggaaccggc ctcgagcccg acgcgcccgt cgttgcgcgc caggattttc
     2401 tcgaccacgc tcttctgctg gttgaccttg atcgggtaga ccgaggtgta atggccgcgg
     2461 taatggtcag cctgcatggc atggtcgaag gcgctgcaca gcgtctcgac gcggtggcgc
     2521 aggatatcgg agaagcgcac cagtaccggc agcgacagac ccgccgcctt gatttcgcgg
     2581 gaaagctcaa catgtcgatg ccggaatgtt cggcgcggcc atcgggccgg cacacgagat
     2641 ggcccttggc gttgatgtcg aagtaaccgc cgctccagtg ggcgatgttg taggtgtcgc
     2701 gggctttctg gatggaccag tcgatcatgg aatgaagtat caagtagcgg tctgcaagta
     2761 tcaagtagcc ggccacgcgc gcggcgtata aaatcgcgca actccccact catccatcac
     2821 acaacatggc gctcgacaaa acctggtaca ccgaggaatg ggccggccaa ggctcagcca
     2881 tctcgctcaa aatcaaggac aaggtgcacg acgagaaatc gccgtaccaa cgcatcgaga
     2941 tctacgaaac tgaaaccttc ggcacgctca tgacgctcga tggtctggtc atggtcaccg
     3001 accgcgacaa cttcgtgtac cacgagatga tgtcgcaccc ggcgctgttc acgcatcccg
     3061 atccgaagcg ggtactgatc atcggcggcg gcgactgcgg cacgctgcgc gaggtgctcc
     3121 ggcaccaatc cgtggagctg gccgagcagg tcgagctcga cgaacgcgtc acgcgcgtgt
     3181 cggaaaagtt ttttcccgaa ttgtgcgaat cgaacaatga tccgcgcgca aaactgcact
     3241 ttgacgatgg gatcaaatgg gtcgcggacg ccaaacccga aacttatgat gtcatcatca
     3301 tcgattcgac cgacccggtg ggaccggcag caggtctttt ttccgaaacc ttctataaaa
     3361 attgcttccg ggcgctgcgg ccgcacggcg tcgtggtcgg tcagagcgaa tcaccgctgt
     3421 tccacgccga
//
LOCUS       DBNGZE010000303         3010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1432511, whole genome shotgun sequence.
ACCESSION   DBNGZE010000303 DBNGZE010000000
VERSION     DBNGZE010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1432511"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="AC16FV_07910"
     CDS             <1..569
                     /locus_tag="AC16FV_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HIR2850015.1"
                     /translation="RFELGAATVDPCIEGFDPSFLKDYVAALGVPYFYRRQDIVGRAL
                     KTMRGDSFCAYCSRMRRGILYATAREQGYNVLALAHHLDDLAESFLMSAFYGGTLRTM
                     KAHYVNDAGDLRIIRPFVYARERQTRDFALRAGLPAIMDNCPACFSMPTQRMHIKSLL
                     SEQEKTQKRLFSNLLTAILPLMSERLPK"
     gene            complement(610..1473)
                     /locus_tag="AC16FV_07915"
     CDS             complement(610..1473)
                     /locus_tag="AC16FV_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR2850016.1"
                     /translation="MKIRIPVVLLLAAALCAPAAHALKLNFDVDKLKEVKGKLKTLKE
                     PSEADEIEIGKGVAANLLGAAAPVNDPQLQAYVNRVGQWVAMHTERPDLPWHFAVLDT
                     NGVNAFATPGGYVFITRGMLLRMRDEAELAGVLAHEVSHVVEKHALKTMRKGALAGLA
                     GDALSSYAEKKGKEEFTKIVSAGTEIYARGLDKEDEFAADRAGVVIAARAGYDPYGLP
                     SVLQTLASINPKDDAVALMFKTHPDSGTRLELVSNSMEGRLDKFVDNPRVESRFSSII
                     KSHIASYKATK"
     gene            complement(1486..2112)
                     /locus_tag="AC16FV_07920"
     CDS             complement(1486..2112)
                     /locus_tag="AC16FV_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR2850017.1"
                     /translation="MKQWITLVSLVGLAALNTACAEETGDKKNAGTATSETTSVAMNS
                     GAAATATATKSTGAAVRETGTTRTAVDLKATPHSDAKGAGQLAANTTVNILERRGGWL
                     RISAASKTGWAKLHQVRVGEGPEAKKSGEGLAILKNVGQTGRSGSQGIVATTGIRGMS
                     AEELKNAKPDPAAVTAIERYRVNASQAREYAKAAGLKEANVPALPKPE"
     gene            complement(2184..>3010)
                     /locus_tag="AC16FV_07925"
     CDS             complement(2184..>3010)
                     /locus_tag="AC16FV_07925"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR2850018.1"
                     /translation="GLSWLVASVALAAAAWAALVQMRMHIPVVVPLLFGGWFYYLLAA
                     LRAWRLERENRQRVTSLFSRFLDPRVVKGLVEKGETDASLSGQKREITVLFSDIQGFT
                     SLSERKSAQEIVNLLNRYFSLQVEVIFRHQGTLDKYIGDAIMAFWGAPTDQPDHARRA
                     LECAREMEQTLMRFRQELGPDGEHFDVGIGIHTGEAVVGFIGSPEHRQDYTVIGDTVN
                     TASRIEGATRGRSRILVSAAVRDTCRAQYRFNDHGLVALKGKEGKVHLYEPTWELS"
BASE COUNT          543 a          923 c          885 g          659 t
ORIGIN      
        1 tgcgtttcga gcttggggcg gcaacggtcg atccgtgcat tgaaggtttt gatccctcgt
       61 ttctcaagga ctatgtcgcg gccttgggcg ttccctattt ctaccgccgc caagacatcg
      121 tcggccgcgc gctcaagacc atgcgcggcg attcgttctg cgcctactgc tcgcgcatgc
      181 ggcgcggcat cctgtatgcc acggcacggg aacagggcta caacgtgctg gcgctggcgc
      241 atcatctgga tgatctggcc gagtcgtttc tgatgagcgc cttttacggc ggcaccctgc
      301 ggaccatgaa ggcgcactac gtgaatgacg ccggcgatct gcgcatcatc cgccctttcg
      361 tgtatgcgcg cgagcgccag acccgggatt ttgcccttcg cgctggtctt cccgccatca
      421 tggacaactg cccagcctgt ttcagcatgc ctacccaacg tatgcacata aaatcgttgc
      481 tttctgagca ggaaaagaca caaaaaagac ttttttcgaa tttattgaca gctattctcc
      541 cacttatgtc tgagagatta ccaaaatgac aatataacaa tgctatgtaa aaagtgggaa
      601 tatttaatac tatttagtcg ctttatagct tgctatgtgg gatttaatta tcgaagaaaa
      661 gcgcgattca acacggggat tatcgacaaa cttatctaaa cggccttcca tggagtttga
      721 aaccagctcc agacgtgttc cagagtccgg gtgggttttg aacatcagag caacggcatc
      781 atccttggga tttatggagg caagcgtctg taacaccgag ggcaaaccgt aggggtcgta
      841 gccggcacgc gccgcaatga ccacccccgc acggtcggcg gcgaattcgt cttccttgtc
      901 caagccgcgc gcgtagatct cggtaccggc gctgacgatc ttggtgaatt cctctttgcc
      961 cttcttctcg gcgtagctgc tcagggcatc gccggcgagc ccggcgagcg cgcccttgcg
     1021 catggtcttg agcgcgtgtt tctccacgac atgcgagact tcgtgggcca gcacgccggc
     1081 gagctcggcc tcgtcgcgca tgcgcagcag catgccgcgc gtgatgaaga cataaccgcc
     1141 cggtgtggcg aaggcattca cgccattggt atcgagcacg gcgaaatgcc aaggcagatc
     1201 agggcgctcg gtgtgcatcg ccacccattg tccgacgcga ttcacatagg cctgcagctg
     1261 cggatcgttc acgggcgccg ccgcgccgag caggttggcg gccacgccct tgccgatttc
     1321 aatttcgtcg gcctcggatg gttccttcag cgtcttgagc ttgcctttga cttccttcag
     1381 cttgtccacg tcgaagttga gcttgagcgc atgcgccgcg ggcgcgcaca gcgcggccgc
     1441 cagcaacaaa acgacaggaa tgcggatttt catgggtgcc tccgtttatt caggtttcgg
     1501 cagggccggg acgttggctt ccttgagccc cgcagccttg gcgtattcgc gcgcctggct
     1561 cgcgttgacg cgatagcgct cgatcgctgt caccgccgcc ggatcgggct tggcgttttt
     1621 aagttcctcg gcgctcatgc cgcgtatgcc ggtggtagcg acgatgccct gcgaaccgga
     1681 acggccggtc tgtccgacgt ttttcagaat cgccagaccc tcgccggatt ttttcgcttc
     1741 cgggccttcg cccacgcgca cctggtgtaa tttggcccag ccggttttgc tcgcggcgga
     1801 gatgcgcagc cagccgccgc gacgctcaag gatgttcacc gtggtgttgg cggccagttg
     1861 accggcgccc ttggcgtcgc tgtgtggagt tgctttcaaa tccaccgctg tgcgcgtggt
     1921 gccggtttcc cgtacggcgg cgccggtgct tttggtcgcg gttgccgttg ccgccgcgcc
     1981 gctattcatg gcgacgctag tggtttccga ggtggcagtg ccggcatttt tcttatcgcc
     2041 ggtttcctcg gcgcaggccg tgttcagcgc ggccagcccg acgagcgata ccagggtgat
     2101 ccattgtttc aaacctgtag ccctgctcgc ttcgcagctg tatttttggg acgctaagac
     2161 tacgctcgct ctttcatctt gattcatgat aactcccatg tgggttcgta cagatgaacc
     2221 ttgccttcct tgcccttgag cgccacaagg ccgtgatcgt tgaagcggta ttgcgcgcga
     2281 caggtatcgc gcacggcggc cgataccaat atacgagaac gtccgcgggt ggcgccctca
     2341 atgcggctcg cggtgttcac ggtgtcgccg atcacggtgt aatcctggcg gtgctccggt
     2401 gagccgatga aacccaccac ggcctcgccg gtatggatac cgatgccgac gtcgaagtgc
     2461 tcgccatcgg gacccagctc ctggcggaac cgcatcagcg tctgttccat ctcgcgcgcg
     2521 cattcgagcg cgcggcgggc gtgatccggc tgatccgtcg gcgcgcccca gaacgccatg
     2581 atggcatcgc ctatgtattt gtccagcgtt ccctgatgcc gaaaaatcac ttcgacctgc
     2641 aaactgaaat aacgattgag caggttcacg atctcctgcg ccgatttgcg ctccgacagc
     2701 gaggtgaaac cctggatgtc ggaaaacagc accgtgatct cgcgtttctg accggaaagc
     2761 gaggcatcgg tttcgccttt ttcgaccaac cctttcacca cgcgcgggtc gaggaagcgc
     2821 gaaaacagcg aggtgacacg ctggcggttt tcgcgctcaa ggcgccaggc gcgcagcgcc
     2881 gccagcaggt aatagaacca gccgccgaaa agcagcggca ccaccaccgg tatgtgcatg
     2941 cgcatctgca ccagcgccgc ccaggcggcc gccgccagcg ccacgctcgc gaccagccag
     3001 gagaggccga
//
LOCUS       DBNGZE010000304         4789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_390468, whole genome shotgun sequence.
ACCESSION   DBNGZE010000304 DBNGZE010000000
VERSION     DBNGZE010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_390468"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /gene="rlmKL"
                     /locus_tag="AC16FV_07930"
     CDS             <1..1642
                     /gene="rlmKL"
                     /locus_tag="AC16FV_07930"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HIR2850019.1"
                     /translation="VQAPLKENLAAAILLRANWPAIAKQGGMLVDLMCGSGTLPIEAA
                     LIAADIAPGLAREYFGFMRWKQHDAAVWTELLDEAHQRREMGLAQLPPIHGYDHDPLA
                     IRSARDNIKRAGLGNLIVAQQRELNDCAPEQVPIGLVVANPPYGERLGPSSELPALYT
                     ELGTQFKKCFPGWRGAVFTGNPELGKVMGLRAGKMHTLYNGAIECKLLHFEITPENVV
                     GERAPKPFEIRPGSSAEMFANRLRKDLQHFGRWARRQEIFAYRLYDADLHEYNLAIDV
                     YESDRRYVHVQEYEAPDTISPDKARQRLQHALGVIPLVLEIPRDQMFFKVRKRQKGGA
                     QYEKFDETGKFHEVREGPCRFLVNFTDYLDTGLFLDHRDTRTMLGQLASGKRFLNLFG
                     YTGTATVHAALGGATSTTTVDMSYTYLDWAQRNLELNQLPANKHELIQADVLVWLKEN
                     RQRRYGLIFLDPPTFSRSKRMEDTLDVQRDHVALIADTTALLEPGGTLIFSTNLRRFK
                     IERDALAGLHVEDISRKTIPKDFERDPKIHQCFRITRR"
     gene            1810..2301
                     /locus_tag="AC16FV_07935"
     CDS             1810..2301
                     /locus_tag="AC16FV_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2850020.1"
                     /translation="MATTVPVKRVHETRTGEMMRPLGIFEEMEQLAERMLPLSWMRPL
                     QMERSFWGGAMPQVDIIDRETELVVRAAVPGFDKDDIEISSTADTVTLRGCAEEEEKE
                     GEEEGEYYRHEIRCEDFLRTIRLPCLVDDSRAKATFKDGMLELTLPKAEAAKRHTVKI
                     EEA"
     gene            complement(2425..2748)
                     /locus_tag="AC16FV_07940"
     CDS             complement(2425..2748)
                     /locus_tag="AC16FV_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850021.1"
                     /translation="MAHTYHELKEMTVAQLREIAKDMEHEAVKGATQMNKDHLLKGLC
                     TALNIEMHEHHEVVGIDKAAIKTKIRALRKKRDEILASKERKELGGILRQIHALKRTI
                     RRATV"
     gene            3019..3966
                     /locus_tag="AC16FV_07945"
     CDS             3019..3966
                     /locus_tag="AC16FV_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR2850022.1"
                     /translation="MKPTRILVGLATLAGLVLATLHVTSDANVTPPVENMPTAPYISA
                     AEIIEYGQPNRLMLQSGVAMIMDDREGVMLYGRNVERQRPIASLTKLMTALVIIEAGL
                     PLDEQIKITREDRDTLRGTRSRLNFNAVFTRYDLLRAALGASDNRAAAALGRTFPGGE
                     EAMFRAMNEKAQKLGMTQTHYVDASGLHNGNVSTANDLVRLVNETQKHQLFHIMTTTP
                     TFSITDRASERQVAFRNTNRLVHRDSWDIGLSKTGYTSAAGNCLVMQTTISDRPLTIV
                     LLNSWGKLSRYGDARRIQHWLQKAERRVPPMLTARAFTS"
     gene            4088..4753
                     /locus_tag="AC16FV_07950"
     CDS             4088..4753
                     /locus_tag="AC16FV_07950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR2850023.1"
                     /translation="MDVLEAMRRRASTRAYLDKPVDRATIESILEAARWAPSGTNAQP
                     WQVVVVTGETKHKISAALLAERQAGRPENPDYSYYPKQWVEPYKSRRVACGLALYQAL
                     KIGRDDKDARLKAWNNNYSFFGAPVGLLFFLDRALEKGSWVDMGMFIENVMLAALGHG
                     LATCPQASIAEYPDIVRKLVNVPDTRALLCGMALGYPDAGAPVNNYRTAREPVASFTA
                     WLT"
BASE COUNT         1004 a         1480 c         1399 g          906 t
ORIGIN      
        1 ggtgcaggca ccgctcaagg aaaacctcgc cgccgcgatc ctgctgcgcg ccaactggcc
       61 cgccatcgcc aaacagggag gcatgctggt ggacctcatg tgcggctcgg gcacgctgcc
      121 gatcgaagcc gcgctgatcg ccgccgacat cgcgcccgga ttggcgcgcg agtacttcgg
      181 ctttatgcgc tggaaacagc acgatgccgc cgtgtggacg gaattactcg atgaggcgca
      241 ccagcggcgc gaaatgggtc tcgcgcagct gccaccgatc cacggctacg accacgatcc
      301 actggcgatc cggtccgcgc gcgacaacat caagcgcgcg gggctgggca atctcatcgt
      361 cgcgcagcaa cgtgagctga acgactgcgc cccggaacag gtcccgatcg ggctggtggt
      421 ggccaaccca ccctacggcg agcgcctggg tcccagttcc gagttgccgg cgctgtacac
      481 cgaactcggt acgcaattca aaaaatgttt tcccggttgg cgcggggcgg tgttcaccgg
      541 caatcccgag ttgggcaaag tcatgggcct gcgcgccggc aagatgcaca cactctacaa
      601 cggcgccatc gaatgcaagc tgttgcactt cgagatcaca cccgaaaatg tcgtgggcga
      661 acgcgcgccg aaaccgttcg agatccgccc cggctcgagc gcggagatgt tcgccaatcg
      721 cttacgcaag gatttgcagc atttcgggcg ctgggcacgg cgtcaggaaa tcttcgcgta
      781 tcgtttgtac gacgccgacc tgcacgaata taacctcgca atagatgtat atgagtcgga
      841 ccgacgctac gtgcatgtgc aggaatacga agccccggac acgatcagcc ccgacaaggc
      901 gcggcagcgg ctgcaacacg ccttgggcgt gattccgctg gtgttggaaa tcccgcgcga
      961 ccaaatgttc ttcaaggtac gaaaacgcca gaaaggcggc gcgcaatatg agaagttcga
     1021 tgagaccgga aagtttcacg aagtccgcga gggaccgtgc cgctttctgg tgaacttcac
     1081 cgattatctc gatacgggat tattcctcga ccatcgcgat acgcgcacga tgctaggtca
     1141 actggcaagc ggaaaacgtt ttcttaatct ctttggctac accggtactg ccacggtgca
     1201 cgcggcgctc ggtggggcca catccaccac gaccgtggac atgtcataca cctatctcga
     1261 ctgggcgcag cggaatcttg agttgaacca gctgccggcg aacaaacacg agctgatcca
     1321 ggccgatgtg ctggtgtggc tcaaggagaa ccggcaacgg cgctacggtt tgatctttct
     1381 cgacccgccg acgttctcac gctccaagcg catggaggac acgctcgacg tgcagcgcga
     1441 tcacgtggcg ctcatcgctg acaccacagc gctgcttgag cccggcggaa cgctgatctt
     1501 ctcgaccaac ctgcgccgct tcaagatcga gcgggatgcg ctcgcgggcc tccatgtgga
     1561 ggacatttcc cgcaagacga tccccaagga cttcgagcgc gatccgaaaa tccaccagtg
     1621 tttccgcatc acgcgccggt gaaccataga ttcttcttat ccggaataaa gaacccggtc
     1681 aggtgtgctt ttgacgggaa tcaatgaaat tattttattt gtgcgtatgt ttattaatgt
     1741 tcgatgatac aaatgtcggc ggaaattagc agaatggccg gcatgatctc atcgaaaagg
     1801 aggtgcatca tggcaaccac tgttcccgtc aaaagggtac atgaaacacg gaccggggaa
     1861 atgatgcgcc cgctcgggat attcgaggaa atggagcaat tggcggaaag gatgctcccg
     1921 ctttcgtgga tgcggccgtt gcagatggag cggtcgttct ggggcggagc gatgccgcaa
     1981 gtggatatca tcgataggga aacggagctc gtcgtacggg cggctgtgcc gggtttcgac
     2041 aaggacgaca tcgaaatctc cagcacggcg gacaccgtga ccctgcgcgg gtgcgcggaa
     2101 gaggaagaga aggaaggcga agaagaggga gagtattacc ggcatgagat tcggtgcgag
     2161 gattttctgc gcaccattcg tctgccttgc ctggtggatg acagcagggc caaggcgacg
     2221 ttcaaggatg gcatgctgga actgacactc cccaaggcgg aagccgccaa gcggcacacc
     2281 gtcaagatcg aagaagcctg aacatccccg actcgtatca aatgaagaac ccggtgacgc
     2341 atcgggttct tttttgcccg cgctccgatc gccggcgcgg ccgggaaatc cgtcccgccg
     2401 gaaccgatcg ggccggatcc tgctttaaac cgtcgcccgc cggatggtgc gcttgagggc
     2461 gtgaatctgc cgcaggatac cgccgagttc cttgcgttct tttgaggcca gaatttcatc
     2521 gcgctttttt cgcaaggccc ggattttggt cttgatcgcc gccttgtcga tgccgaccac
     2581 ctcatggtgc tcatgcatct cgatattgag cgccgtgcac aagcccttca gcaggtggtc
     2641 cttgttcatc tgtgtcgcgc ccttgaccgc ctcatgctcc atgtccttgg cgatctcgcg
     2701 cagctgcgcg acggtcattt ccttcagttc gtgataggta tgcgccatga tgctgctcct
     2761 tgtcagtgat gtatccgggg aagacggcca cagcttataa acccccgccc gccgtatcaa
     2821 gcggcgcccc cgccatccgg gaccgaaacc gccggtcggt gggacgcccc accgatgagt
     2881 ggcggtcggg agtggaaata cgttccttcg cgcggcttca caagacccat gccggcgggt
     2941 taaggttgcg tcctgccaac agactactaa tatgaagtgg tggcagcgaa aaaccgaaag
     3001 ggaggggttg ttattttgat gaaaccgaca aggattctgg tcggcctggc gactctcgcc
     3061 gggcttgttc tcgcgacgct tcacgtcacc tcggacgcca acgtcactcc gccggttgaa
     3121 aacatgccga cggctccgta catctcggcg gcggaaatta tcgagtatgg ccaacccaac
     3181 cggctcatgc tgcaatccgg cgtggccatg atcatggatg accgcgaagg cgtcatgctc
     3241 tacgggcgca acgttgagcg tcagcgcccc atcgcctccc tgaccaaact catgacagcg
     3301 ctggtcatca tcgaagcggg gttacctctc gacgagcaaa tcaaaatcac gcgtgaagat
     3361 cgtgacacct tgcgcggcac gcgctcgcgc ttgaacttca atgccgtgtt cacgcgttac
     3421 gacctgctgc gcgcggcgct cggtgcttcc gacaaccgcg ccgcggcggc gctggggcgc
     3481 accttccccg gcggcgaaga agccatgttc cgggccatga acgagaaagc acaaaaactc
     3541 ggcatgacgc agacgcatta cgtggacgcc agcggcctgc ataacggcaa tgtctcgacg
     3601 gccaatgatc tcgtgcgact cgtcaacgag acgcaaaaac atcagttgtt tcatatcatg
     3661 acaaccacgc cgacgttcag cattaccgat cgggcctcgg agcgccaagt cgcgtttcgc
     3721 aacaccaacc gcctcgtgca tcgcgacagc tgggacatcg gcctcagcaa aaccggctac
     3781 acctccgccg ccggcaattg cctggtcatg caaaccacca tttccgatcg gccgctcacc
     3841 atcgtgctgc tcaattcgtg gggcaagctc agccgctacg gtgacgcccg ccgcatccag
     3901 cattggctgc aaaaggccga gcgccgcgta ccgccgatgc tcaccgcgcg cgccttcacc
     3961 agctgatcga atcgtttctc ctgcaaacag cccggcacat gccgggcttt ttattttggg
     4021 aattcttccg ctgacgtaat cgggttacat tacgctctct acgatccgca cccgggagct
     4081 tccgatgatg gatgtccttg aagcgatgcg ccgccgcgcc tcgacgcgcg cctacctcga
     4141 caaaccagtg gaccgcgcca cgatcgagtc gatcctggaa gccgcgcgct gggcgccttc
     4201 cggcaccaac gcccagccat ggcaggtcgt ggtggtgacg ggtgaaacca aacataaaat
     4261 cagcgcggca ctgctggcgg aacgtcaggc cggccggccg gaaaatcccg attactccta
     4321 ttacccgaaa caatgggtcg aaccttacaa gtcgcggcgc gtggcctgcg ggctggcgct
     4381 ctatcaggca ctgaaaatcg gacgtgacga caaggacgcg cgcctcaagg cctggaacaa
     4441 caattattcc ttcttcggcg cgccggtggg actgctgttc tttctcgacc gtgcgctcga
     4501 aaaaggttcc tgggtggaca tgggtatgtt catcgagaac gtcatgctcg ccgcgctcgg
     4561 gcatggcctc gccacctgcc cgcaggcctc gatcgcggaa tatcccgaca tcgttcgcaa
     4621 actggtgaat gtccccgaca cccgcgccct cctctgcggc atggcgctcg gctatcccga
     4681 tgccggcgcg cccgtgaaca actaccgtac cgcgcgcgaa ccagtggcga gcttcaccgc
     4741 ttggctaacg tagaacgcat attgaagaat cccccgccct ccgtcccgg
//
LOCUS       DBNGZE010000305         6028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_530341, whole genome shotgun sequence.
ACCESSION   DBNGZE010000305 DBNGZE010000000
VERSION     DBNGZE010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_530341"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16FV_07955"
     CDS             <1..342
                     /locus_tag="AC16FV_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR2850024.1"
                     /translation="RRFYSDLLRTGVRIFEYQPRFLHAKTVLCDNWASIGSSNLDRWN
                     LRWNLEANQEVDDAAFAEAVRSMFEEDFRNSAECRYEDWRRRPWHVRWRERWWGRVDM
                     WLESLGRRKGR"
     gene            440..979
                     /locus_tag="AC16FV_07960"
     CDS             440..979
                     /locus_tag="AC16FV_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HIR2850025.1"
                     /translation="MPTMKKYFVISALGKDRPGIVHELSKAILDSGCNVEDSRMTVLG
                     GEFALILMVSGHWGAITRLERQLPPLEKKLELTILSKHTEPRTGAQDRVPYTVDVVAM
                     DHPGIVHEVADFFASREINIEEMGTWTYPAAHTGTSMFSLNMTVSIPADVHISRLRDE
                     FTSFCDNLNLDATIEPSRH"
     gene            complement(1098..2048)
                     /locus_tag="AC16FV_07965"
     CDS             complement(1098..2048)
                     /locus_tag="AC16FV_07965"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HIR2850026.1"
                     /translation="MSNKYPIVAVTGSSGAGTTTVRHAFQDIFRREGISADFVEGEAF
                     MRYGREEMDRVIRQAQTEGKLLSHFGPEVNQFDRLEALFKEYAERGTGQVRHYVAEDN
                     SEELGLPPGTFTPWRPIPQGTDLLFYEGLHGGVVARNWTRRKLSPSHNPVVVERRKAG
                     DGNNGVDVAQYVDLLIGVVPVVNLEWIQKIHRDTSVKGHTPEAVTSAILRRLRDYTHF
                     ITPQFSISDINFQRVPVVDTSNPFIARDVPAPDESVVVIRFRDPWKFDFPNLMKRIHE
                     SFMSRPNTMVIPGGKMHLALEIICTPLVHELLEKRNKKNT"
     gene            complement(2216..2746)
                     /locus_tag="AC16FV_07970"
     CDS             complement(2216..2746)
                     /locus_tag="AC16FV_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850027.1"
                     /translation="MKCYTVTKDGVVPGIQFLRKPYPHVAVGDPALSSADCRRVEVDT
                     ALADSATDNTIKACSYVIDMKNSEHQRSSYKLVAADGSDDDRMLVKIEARCAAPGQRT
                     FYDLPLNTLALANGWYLAGGKGPQAETPVNLIVLEKGDEIKIHRTVDMRKPAELVFSA
                     GFDDSELRQHQQRAAA"
     gene            2832..3161
                     /locus_tag="AC16FV_07975"
     CDS             2832..3161
                     /locus_tag="AC16FV_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850028.1"
                     /translation="MATIATFFLGILFSRMIGRNTSRKHKKFLIEIFPRRSSLIRAWT
                     FMRSTPKAGQEDIPVCYEKWNESEQVNKALEKAKKEADQMIEKAKTAPRPEPEKETRR
                     ETEPEAA"
     gene            complement(3228..4352)
                     /locus_tag="AC16FV_07980"
     CDS             complement(3228..4352)
                     /locus_tag="AC16FV_07980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR2850029.1"
                     /translation="MNALVRHLEHLQRHPDGGELLQLIKDALHHCRERGLVNGSCLSH
                     LHQQLLAYTQNNAVPSPIRLRARLIQQHLAIYLPQPDRIIPVREPTPAPKAAVPVPVK
                     TAPKPRALKAPDSQLTEMTPAMESPRTPEQKSAPAARTPEAPRAAEADTPPQLDELRQ
                     MLAKGMDEMMRERETLSRQLSDATTYLKMIEAEREQLREELNKARRRIRAGDKSVKRA
                     LGLPKRDVLVRQIESEVERVKRHGEPLALALIDIDNLEDIQQKHGAEIADAVLNRYTS
                     EVLGRFRSYDMVARYNKNEFAVLLPNTGKDNALRALEKVHKRAKESHFSHKGRSYPLP
                     GFGGVLTFYSPGEEPHQMLRRADEALVNLKLRGERQLVIV"
     gene            complement(4697..5509)
                     /locus_tag="AC16FV_07985"
     CDS             complement(4697..5509)
                     /locus_tag="AC16FV_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR2850030.1"
                     /translation="MDIRNEIPARDIAQFYAKALEFSALARALILSLLAAGFEVKRKP
                     DRSFVTSADLKVEERLRELIGKHFPDHGIIGEEYPPTRPAAPFQWIMDPIDGTEDFVQ
                     RMPAFGSILALHYRGEPVVGVIDHPVLDIRVSAALGLGAHKNGERLTLADLDPSAIDG
                     TERVMLPARANFIKHRDDGQLFDAVARAHPNHRIYRTCFTHTCAILGQADAAIDYGNP
                     IWDLAASRILIEEAGGKFLSVRAWDVPSIGRVYGSIMGKPALVDRLATHFNR"
     gene            complement(5514..5702)
                     /locus_tag="AC16FV_07990"
     CDS             complement(5514..5702)
                     /locus_tag="AC16FV_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850031.1"
                     /translation="MADALRRVPMVAGAAASPYANITNLPNSARRYLLPPTREFYKVA
                     WTTVKKQCFKTGKDGVRK"
BASE COUNT         1138 a         1744 c         1872 g         1274 t
ORIGIN      
        1 cggcgcttct acagcgactt gctgcgcacc ggtgtgcgca ttttcgagta ccagccgcgg
       61 tttctgcacg ccaagaccgt gctgtgcgac aactgggcct cgatcggctc gagcaatctc
      121 gaccgctgga acctgcgctg gaatctcgaa gccaatcagg aagtggatga cgccgcattc
      181 gcggaggcgg tgcggtccat gttcgaagag gatttccgca acagcgccga gtgccgttac
      241 gaggactggc gccggcgtcc ctggcacgtt cgctggcgcg aacgctggtg gggcagggtg
      301 gacatgtggc ttgagagtct gggacgccgc aaggggcgct gaaggctgcc gggttcagcg
      361 cgtgacggcg cgcgactggc cgggagcagt gcttgacgct aaaatccgcg ttccatacgg
      421 gcgatcgagg cccgattcca tccccaccat gaaaaaatat ttcgtcatct ccgcgctcgg
      481 caaggaccgt cccggtatcg tccacgaatt atccaaggcc atcctcgata gcggttgcaa
      541 cgtcgaggac agccgcatga ccgtgctggg cggcgagttc gccctgatcc tgatggtttc
      601 cggccactgg ggcgcgatca cgcgcctgga acggcagttg ccgccgctgg agaaaaaact
      661 cgagctgacc atcctgtcga agcacaccga gccgcgcacg ggcgcgcagg accgcgtgcc
      721 ctacacggtg gacgtggtgg ccatggatca ccccggcatc gtgcacgagg tcgcggattt
      781 tttcgccagt cgcgagatca atatcgagga gatggggacc tggacttatc ccgccgcgca
      841 caccggcacg tccatgtttt cgctcaacat gaccgtaagc atcccggccg acgtgcacat
      901 cagccgcctg cgcgacgagt tcacgagttt ctgcgacaac ctcaacttgg acgccaccat
      961 cgagccgtcg cggcattaga attatcttta tccgcagatt aacgcggatt catcttatta
     1021 agatgaacgc agataaaaca ataatttgtc tgattcatcc gcgtttatct tcataatcat
     1081 ctgcgtttat ctgcgtttca ggtgtttttc ttattccgct tttccagcag ctcgtgcacc
     1141 agcggcgtgc aaatgatttc cagcgccaaa tgcatcttgc cgcccgggat gaccatggtg
     1201 ttcgggcgcg acatgaacga ttcgtggatg cgcttcatca ggttgggaaa gtcgaatttc
     1261 cacgggtcgc gaaagcgaat gaccaccacg ctttcatccg gcgccggcac gtcgcgcgcg
     1321 atgaaggggt tggaggtgtc caccaccggc acgcgctgaa aattgatgtc actgatggaa
     1381 aactgcggcg tgatgaagtg cgtgtagtcg cgcaggcgcc gcaggatggc gctggtgacg
     1441 gcctcgggcg tatgtccctt gacgctggtg tcgcggtgga ttttctggat ccattcgaga
     1501 ttcaccaccg gcaccacccc gatcagcagg tccacgtact gggccacgtc cacgccgttg
     1561 ttgccgtcgc cggccttgcg ccgttcgacg accacgggat tgtgcgacgg gctcaatttg
     1621 cgccgcgtcc agttgcgcgc caccacgccg ccgtgcagtc cttcgtagaa cagcaagtcg
     1681 gtcccctggg ggatcggtcg ccacggggtg aaggtccccg gcggcaggcc gagttcttcg
     1741 ctgttgtctt cggcgacgta gtgccgcacc tgaccggtgc cgcgttcggc gtattcttta
     1801 aaaagagctt cgaggcggtc gaactgattc acttccggcc cgaagtgact cagtaatttc
     1861 ccctcggttt gtgcctgccg gatgacacga tccatctcct cgcgcccata acgcatgaag
     1921 gcctcgccct cgacgaaatc ggccgagatg ccctcgcgtc ggaaaatatc ctggaacgca
     1981 tgacgcacag tggtggtgcc ggcgcccgag gagccggtga ccgcgacgat gggatacttg
     2041 ttggacatgg tctgaacccg gacgcccgtg atgaatgtcg ctatcttctt ggttttcggg
     2101 tgcgccggtc aacggtggcg ggatgaaccc gtcgagacac gcgcccagcg gcaaaataaa
     2161 cgccggggcg gggtgcccca gccatctgga atgagtcaaa accgccgcaa gcccttcagg
     2221 ccgcggcgcg ctgctggtgt tgtctcagtt cggagtcgtc gaagccggca ctgaagacca
     2281 attcggcggg tttgcgcatg tccaccgtcc ggtgaatttt gatttcatcg cctttctcga
     2341 gcacgatcag attgaccggc gtttccgcct gcggcccctt gccgccggcg agataccacc
     2401 cgttcgcgag cgccagggta ttgagcggca ggtcgtagaa ggtgcgttga ccgggcgcgg
     2461 cgcagcgcgc ttcgatcttg accagcatcc tgtcgtcgtc actgccatcg gcggcgacca
     2521 gcttgtagga agagcgctgg tgttcggaat ttttcatatc aatgacgtaa gagcaggctt
     2581 tgatagtgtt gtcggtggcg ctgtccgcga gcgcggtgtc cacttccacg cgccggcagt
     2641 cggcggatga cagggccggg tcgccgaccg ccacgtgggg ataaggtttg cgcaggaatt
     2701 gaatgccggg aacaactccg tctttcgtaa cggtgtagca tttcatgttt gtctccttct
     2761 tgatggattg tcagtgttga aacaacagga ccgaaaaggg acggttagat tttttaccca
     2821 atcgcgcatg cttggcaacc attgccacgt tttttctggg tattttgttt tcgcgcatga
     2881 tcgggagaaa cacgtcaagg aaacataaaa aatttttaat tgaaattttc cctcgccgga
     2941 gtagcttgat tcgtgcatgg acgttcatgc gaagcacgcc caaggcaggg caggaggata
     3001 tccccgtgtg ctacgaaaaa tggaatgaga gcgaacaggt gaacaaggcc ctggaaaagg
     3061 ccaaaaaaga ggcggatcag atgatcgaaa aggcgaagac cgccccgcgt cccgagccgg
     3121 agaaggaaac ccggcgcgag acagagcccg aagccgcctg aagcgttctt tatggtgtca
     3181 aatttcaaac cccgcgcccg cggggtttgt tttttcaggg acgccgctta aacgatgacg
     3241 agctggcgct cgccgcgcag tttcagattt accagcgcct cgtcggcgcg gcgcagcatc
     3301 tggtgcggct cctcgccggg ggaatagaag gtgagcacgc cgccgaagcc gggcagcggg
     3361 tagctgcggc ccttgtggct gaaatgcgat tccttggcgc gcttgtgcac cttctccagc
     3421 gcgcgcaggg cgttgtcctt gccggtgttg ggcaacagca cggcgaattc gtttttgttg
     3481 tagcgcgcaa ccatgtcgta gctgcggaag cggccgagca cctcgctggt gtagcggttc
     3541 agcaccgcgt cggcgatttc cgcgccgtgc ttttgctgga tgtcttccag attgtcgatg
     3601 tcgatcagcg ccagcgccag cggctcgccg tgacgcttca cgcgctcgac ctcggattcg
     3661 atctgccgca ccagcacgtc gcgcttgggc aggccgagcg cccgtttcac ggatttgtcg
     3721 ccggcccgga tgcgccggcg cgccttgttg agttcctcgc gcaactgttc gcgttcggcc
     3781 tcgatcatct tgaggtaggt ggtggcgtcg gagagctggc gcgacagcgt ctcgcgctcg
     3841 cgcatcatct cgtccatgcc cttcgccagc atctggcgca gttcatcgag ttgcggcggt
     3901 gtatcggcct cggcggcgcg cggcgcctcc ggtgtcctgg cagccggcgc cgatttttgt
     3961 tccggcgttc ttgggctttc catggcaggg gtcatctccg tgagctggga atcgggagcc
     4021 ttgagcgcgc gcggttttgg tgcggtcttg acaggaacag ggacggccgc cttcggcgct
     4081 ggggtcggtt cgcgcaccgg gataatccgg tccggttgcg gcagatagat cgccagatgc
     4141 tgctggatca ggcgcgcccg caggcggatg ggcgagggca ccgcgttgtt ctgggtgtag
     4201 gcgagcagct gctggtgcaa atgggaaaga caggaaccgt tcacgagacc acgctcgcgg
     4261 cagtgatgca gggcatcctt gatcaactgc aacagttcgc cgccgtccgg atggcgctgc
     4321 aggtgctcaa ggtgccgcac caaagcgttc attattgatc cggcactact gttctcaagc
     4381 gaaggcaact tgttcacttt aacagtccac tctctcaggt tttaatgcgc cggatcaata
     4441 atgagaacct cttaagattg cttgttattg cggcggcaaa gcgctttatg tgcaaacacc
     4501 cgatccctaa ttgggtcggt gcttcaaatg ggttcgcgcc gccgcaataa ccggggtcga
     4561 cggagaggtt ttcgccgaca gccaacctgt gtgctcctag ggggttcggg gacctttatt
     4621 actttcttgt cgcgcgtaaa tgtaaccgta caagtgaaaa ataacaatat cttgtgatga
     4681 aaaaatatga atagcactac cggttgaaat gagtcgccaa ccgatcgacg agcgctggct
     4741 tgcccatgat cgaaccgtac acgcgtccga tcgaaggcac gtcccaggcg cgcacgctca
     4801 gaaacttccc gcccgcctct tctataagaa tacggctggc ggccaggtcc cagatggggt
     4861 tgccgtaatc gatggcggca tcggcctggc ccagaatcgc gcaggtatgg gtgaaacagg
     4921 tgcggtaaat gcggtgattc gggtgggccc gcgccacggc gtcgaacagc tgcccgtcgt
     4981 cgcggtgctt gatgaagttc gcccgcgccg ggagcatgac gcgctcggtg ccgtcgatgg
     5041 cggaggggtc gaggtcagcc aaggtcaggc gttcgccgtt cttgtgggcg ccaagaccga
     5101 gcgccgcgct cacgcggatg tccagcaccg gatggtcgat gacgcccact actggttcgc
     5161 cgcggtagtg cagcgcgaga atgctgccga aggccggcat gcgctgcacg aaatcctcgg
     5221 tgccgtcgat cgggtccatg atccactgga acggcgccgc gggacgggtc ggcgggtatt
     5281 cctcgccgat gatgccgtga tcgggaaaat gtttgccaat gagttcgcgc agccgctcct
     5341 cgaccttgag atcggcgctg gtgacaaagc tcctgtccgg cttgcgtttg acctcgaacc
     5401 cggctgctag cagggacagg atcagcgccc gcgccaacgc ggaaaattcc agcgccttcg
     5461 cgtaaaactg tgcgatgtcc cgcgccggga tttcgttgcg gatgtccacg gaattatttc
     5521 ctcaccccat cttttccagt tttaaaacat tgtttcttca ccgtggtcca cgcgacctta
     5581 taaaactccc gcgtgggcgg cagcaggtag cgccgcgccg aattcggcag gttggtgata
     5641 ttggcataag gcgaggctgc tgcaccagca accatcggaa cgcgcctcag cgcgtccgcc
     5701 actgtttcag gaatgccgtc tcccgggttg tcgcgggcgt tgcctcgtgt tttgtccccg
     5761 aacgcttttt cagccccttg ccgttaagga acctctgatt aatttcgttc gtggtgagct
     5821 tgtcgaacca cgaacgggca gggacatgat ccattcaatc gctttccgtt cgcccttcga
     5881 caggctcagg gcgaacgggc gaataaatca gaggttcctt aaaaaagaaa ttggaggccg
     5941 accgaagcgg tgtcaagatc ggtgtgcgct acgtcgtagc gttcccactc caggcgtaag
     6001 gccagatttc tggtaaagcc gagattga
//
LOCUS       DBNGZE010000306         6547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1571455, whole genome shotgun sequence.
ACCESSION   DBNGZE010000306 DBNGZE010000000
VERSION     DBNGZE010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1571455"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="AC16FV_07995"
     CDS             <1..586
                     /locus_tag="AC16FV_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2850032.1"
                     /translation="EIEQRGSPQPALLPLDLATATPSHYEQVAQTIAREVGGLDGLLH
                     NAADAGTLTPLEHYMPETWFRVMQVNLNAAWLLTRACLPLLHQSADASIVFTTAEVGR
                     KGRAYWGAYGVSCFGLEGLTQILADECAVEGRIRVNSLDPGTVRTGMRSRLYPGEDRA
                     TLPAPETLMPAYLYLLGPDSRGVNGQALNAQNFL"
     gene            862..3303
                     /gene="nirB"
                     /locus_tag="AC16FV_08000"
     CDS             862..3303
                     /gene="nirB"
                     /locus_tag="AC16FV_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HIR2850033.1"
                     /translation="MEKEKLVLVGNGMAGMRTLEELLKTAPDMYDITVFGSEPYGNYN
                     RILLSPVLAGEKAFHEILLNDEPWYETNGITLHKNCEVVEINRRARKVVARDGTTATY
                     DRLLLATGSHPFILPVPGSTLPGVITFRDINDVDTMISASKSLRHAVVIGGGLLGLEA
                     ANGLKQRGMYVTVVHLSRTLMDRQLDEVAGGMLHRSLVERGVEFLLGAETGEIIGKKR
                     VQGVRFKDGREITADLVVMAVGIRPNIDLAKKAGLYCERGIIVNDTLQTYDPRVYAVG
                     ECVQHRRQTYVLVAPLFEQAKVCADHLARLGIRRYQGSVTSTKLKVTGIDVFSAGDFA
                     GNEQTESIVLQDPGVGVYKKVVLKDNRIQGAVMVGDTVDGPWYFQLMREGTDISAFRT
                     RLLHGQAHLGDAGHGGANAVAAMDDKAEVCGCNGVCKGEIVQAIVGKKLFTLEDVRAH
                     TKASSSCGSCTGLVEQILAMTLGGDYSAAPKAKPLCKCTEYTHDEVRAQIRERHLTSI
                     GAVMQALEWKKPDGCAACRPALNFYLLATWPAEAKDDYQSRFINERAHANIQKDGTYS
                     VVPRMWGGVTTPAELRAIADVAEKFEVPEVKVTGGQRIDLLGVTKEQLPAIWGELGKA
                     GLVSGHAYGKAIRTVKTCVGSEWCRFGVQDSTNMGIQIEKMTWGSWTPHKFKMAVSGC
                     PRNCAEATIKDFGVVAVDSGWELHVGGNGGVKVRVTDLLCKVATPEEVLEHCGAFLQL
                     YREEARYLERTAPWVERVGLASIKQKIVENPAERKALYARFLESQKYAQVDPWAERAA
                     DGVDRHEFIPLKQIA"
     gene            3312..3653
                     /gene="nirD"
                     /locus_tag="AC16FV_08005"
     CDS             3312..3653
                     /gene="nirD"
                     /locus_tag="AC16FV_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HIR2850034.1"
                     /translation="MNNWIEIGRIEDIPSQGARVIRTSQGDIAVFRTATDEIFALRDR
                     CPHKGGPLSQGIVSGKTVTCPLHNWNIALDTGEAVAPDEGCAGHFPVKVVDGKISLLL
                     SAERVKLAVVC"
     gene            3750..4736
                     /gene="moaA"
                     /locus_tag="AC16FV_08010"
     CDS             3750..4736
                     /gene="moaA"
                     /locus_tag="AC16FV_08010"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR2850035.1"
                     /translation="MEPRLIDSFNRRIEYIRLSVTDRCDLRCTYCLPAGYHDFIEPEH
                     WLTFDEIERVIRAFTELGVYRVRLTGGEPLTRHNLPELAARLNALPQLDDLSLSTNGV
                     RLKKQAHTLRQAGVKRINVSLDSLQPERFAAITGGGKLDKVLDGLTAASEAGLQPVKI
                     NMVVMNGVNDDEVEDIVQYCIDRDFTLRLIETMPMGDTGRTASSHYLDLEVVRRRLEQ
                     KFKLVPAVMPGGGPARYVGVAGTNLRIGFITPISRHFCETCNRVRLSADGALYLCLGQ
                     DDKVELRPLLRAGISDEDLKDTIRAAIARKPERHEFREQPQKIVRFMSRTGG"
     gene            complement(4885..5367)
                     /gene="rsd"
                     /locus_tag="AC16FV_08015"
     CDS             complement(4885..5367)
                     /gene="rsd"
                     /locus_tag="AC16FV_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HIR2850036.1"
                     /translation="MTTEHKREMTPQENRAKERRTGSQELVQKLVTERTEMLSLYCQL
                     AGLEPYGNGKNPRVKHSQELLQKFCQVLVDYIAAGHFSLYERIVNGTERRQQISALAE
                     NLYPRIAKSTEAALDFNDKYDCGDHCEIAASFSDDLSRLGEELAARIELEDKLLRQLH
                     "
     gene            complement(5483..>6547)
                     /locus_tag="AC16FV_08020"
     CDS             complement(5483..>6547)
                     /locus_tag="AC16FV_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR2850037.1"
                     /translation="RPPAVILVAGLQGSGKTTTVAKLARFLREREKKSVMVVSADVYR
                     PAAIDQLQKLAAAVGAEFFPSRPDQKPVDIARQAVEEARKRLMDVLIVDSAGRLHVDQ
                     PMMQEIRAVHAALEPVETLFVVDSMTGQDAVNTAKAFNEALPLTGVILTKTDGDARGG
                     AALSIRQVTGKPIKFMGVGEKPAALEPFYPDRVAARILGMGDVLGLVEQATQAADKEK
                     AQEFAQKLVKGKGFDLEDFRDQLLQMEKMGGLASLMEKLPGVGEVSEVAKSQLGDRQV
                     RRLVAIINSMTPQERNHPDTIRGSRKRRIAAGSGTQVQDVNRLLKQFDQMQKVMKRMG
                     KGGLQRMLRDVKGRLPGMPG"
BASE COUNT         1285 a         2052 c         1968 g         1242 t
ORIGIN      
        1 cgaaatcgag caacgcggct cgccgcagcc ggcgctgctg ccgctggatc tggccacggc
       61 cacgccttca cattatgagc aggtggcaca aaccatcgcg cgggaagtcg gcggcctcga
      121 cggcctgctg cacaacgccg ccgatgccgg cacgctcacg ccgctggagc attacatgcc
      181 ggagacgtgg ttccgcgtga tgcaggtaaa tctgaacgcc gcctggctgc tgacgcgcgc
      241 ctgtctgccg ctgctgcacc aatccgcgga tgcctcgatc gtcttcacca ccgccgaggt
      301 cggacgcaag ggacgtgcct actggggcgc ttatggcgtt tcctgcttcg ggctggaagg
      361 gctgacgcag attctcgccg acgagtgcgc cgtcgagggg cgtatccgcg tcaacagtct
      421 cgaccccggc acggtccgca ccggcatgcg ctcgcgcctc taccccggcg aggatcgcgc
      481 caccctgccc gcgccggaaa ccctcatgcc cgcctatcta tacctgttgg gcccggacag
      541 ccgcggtgtg aacgggcagg cgctgaacgc ccagaacttc ttgtaaacga gaatagaggt
      601 agcccggatg aagcgcagcg gaatccgggg ctgatgcccg gattccattt cattccatcc
      661 gggctacata aatgatccaa atcgtgcatc cccactcctc catgcaccga gagagtgcaa
      721 acgcctggcg ctcgccgggc gcggcgtaac tcgcaccggt ttcaattcaa caagttatct
      781 gttcgcccgt gcgcgcgcac gctggcacgc cggttgctat gtgggcgata gcgttataac
      841 catttcgaca ccgcggagcc catggaaaaa gaaaagctgg tattggtcgg caacggcatg
      901 gccggcatgc gcacgctcga agagctgctc aagaccgcgc cggacatgta cgacatcact
      961 gtgttcggct ccgaaccgta cggcaactac aaccgcatcc tgctttcgcc cgtactcgcg
     1021 ggcgagaagg cctttcacga aatcctgctg aatgacgagc cgtggtacga aaccaacggc
     1081 atcacgctgc acaagaattg cgaagtcgtc gaaatcaacc ggcgggcacg gaaagtcgtc
     1141 gccagggatg gcactaccgc cacgtacgac cggctgctgc tggccaccgg ctcccatccg
     1201 ttcattctgc cggtgccggg cagcactctg cccggtgtca tcaccttccg cgacatcaac
     1261 gacgtggaca ccatgatcag cgcatccaaa tcgctgcgcc atgccgtcgt catcggcggc
     1321 gggctgctgg gtctggaagc ggccaacggg ctgaagcagc ggggcatgta cgtcaccgtg
     1381 gtacaccttt caagaacgct gatggatcgc cagctggacg aggttgcggg cggcatgctg
     1441 caccgctcgc tcgtggaacg cggcgtggaa ttcttgctcg gcgcggaaac cggggagatc
     1501 atcggcaaga agcgcgtgca aggcgtgcgc ttcaaggacg gacgggaaat taccgccgac
     1561 ctcgtggtca tggcggtcgg catacggccc aatatcgatc tggcgaagaa agccggtctg
     1621 tactgcgagc gcggcatcat cgtcaacgac acgctgcaaa cctacgaccc gcgcgtgtat
     1681 gccgtgggcg agtgtgtgca gcaccggcgc cagacttacg tacttgtggc gccgctgttc
     1741 gaacaagcca aggtctgcgc cgatcatctg gcccggctcg gcatccgccg ctatcagggc
     1801 tcggtcacct cgaccaagct caaggtcacc ggcattgacg tgttttccgc cggcgatttc
     1861 gccggcaacg agcagaccga gagcatcgtg ttgcaggacc cgggcgtggg cgtttacaaa
     1921 aaggtcgtgc tgaaggacaa ccgcattcag ggcgcggtca tggtgggcga caccgtggac
     1981 ggtccatggt acttccagct catgcgcgaa ggcacggaca tcagcgcctt ccgcacgcgc
     2041 ctgctgcacg gtcaagcgca cctgggtgac gccggccacg gcggcgccaa cgccgtcgcg
     2101 gccatggacg ataaggccga agtctgtggt tgcaacggcg tgtgtaaggg cgagatcgtc
     2161 caggccatcg tcggcaaaaa actatttact ctcgaggacg tgcgcgccca caccaaggcc
     2221 tcgtcctcct gcggttcctg caccgggctg gtggaacaaa tactggccat gacgctcggc
     2281 ggcgattatt ccgccgcgcc caaggcgaag ccgctgtgca agtgcaccga atacacccac
     2341 gacgaggtgc gcgcgcagat ccgcgagcgc cacctgacca gcatcggcgc ggtgatgcag
     2401 gcgctggaat ggaaaaaacc ggacggctgc gccgcctgcc gcccggcgct gaacttttat
     2461 ctcttggcca cctggccggc ggaagcgaag gacgattacc aatcgcgctt catcaacgaa
     2521 cgcgcgcacg ccaacatcca gaaagacggc acctattcgg tagtgccgcg catgtggggc
     2581 ggcgtgacca caccggcgga actgcgcgcc atcgccgacg tcgcggagaa attcgaggtg
     2641 cccgaggtca aggtcaccgg cggccagcgc attgacctgc tgggcgtgac gaaggaacag
     2701 ctgcccgcca tctggggcga gctcggcaag gccgggctgg tctccggcca cgcctacggc
     2761 aaggccattc gcaccgtgaa gacctgcgtc ggctccgagt ggtgccgctt tggcgtgcag
     2821 gattcgacca acatgggcat tcagatcgag aaaatgacct ggggctcctg gacgccacac
     2881 aagttcaaga tggcggtgtc gggttgtccg cgcaattgcg ccgaggccac catcaaggac
     2941 ttcggtgtcg tggccgtgga ctccggctgg gaactgcacg tcggtggcaa cggcggcgtg
     3001 aaggtgcgcg tcaccgacct gctgtgcaag gtcgccacgc ccgaggaagt actggaacat
     3061 tgcggcgcct tcctgcaact ctatcgcgag gaggcgcgct atcttgaacg caccgcaccg
     3121 tgggtcgagc gcgtgggcct ggcctcgatc aagcagaaga ttgtcgagaa ccccgcggaa
     3181 agaaaggcgc tgtatgcgcg ctttctggaa tcccagaaat acgcgcaggt ggacccgtgg
     3241 gccgaacgcg ccgccgatgg cgtggaccgg cacgaattta tcccactcaa acaaattgcc
     3301 tgatgaacct catgaacaac tggatcgaaa ttggccgcat cgaggatatc ccttcccagg
     3361 gcgcgcgcgt gatacgcacc tcgcagggcg acattgcggt tttccgcact gccacggacg
     3421 aaatcttcgc cctgcgcgac cgctgcccgc acaagggcgg accgctgtcg cagggcatcg
     3481 tgtccggcaa aaccgtcacc tgccccttgc acaactggaa catcgccctc gacaccggcg
     3541 aggccgtggc gccggacgag ggctgtgccg ggcatttccc cgtcaaagtc gtggatggaa
     3601 aaatctcgct cctgctttcg gcggaacggg taaaactggc cgttgtctgt tgaacaactc
     3661 cgccggattc ctgtctctgg gtataccagg ctaatcacct attatttcag gggtgtaccg
     3721 cgcgctgcgg tactcttata gtgtcgtaca tggaaccccg gctgatcgac agcttcaacc
     3781 gcagaatcga atacatccga ttgtcggtca ctgaccgctg cgatctgcgc tgcacctatt
     3841 gcctgccggc gggttatcac gatttcatcg agcccgaaca ttggctgacc ttcgacgaaa
     3901 tcgaacgcgt gatacgcgcc ttcaccgagc tcggcgttta ccgcgtgcgt cttaccggcg
     3961 gcgaaccgct gacgcgtcac aatctcccgg aactggcggc acgcctgaac gcgctcccgc
     4021 aactcgatga cctctcgctc agcaccaacg gcgtccggct gaaaaaacag gcccacacgc
     4081 tcagacaggc cggcgtaaaa cgtatcaacg tcagcctcga ctcgttacag cctgagcgtt
     4141 ttgccgccat caccggtggc ggcaagctcg acaaagtgct ggacggcctc accgccgcca
     4201 gcgaggccgg cctgcaaccg gtcaagatca acatggtggt gatgaacggc gttaacgacg
     4261 acgaggttga ggacatagtg caatactgca tcgatcgcga tttcacgttg cgcctgatcg
     4321 aaaccatgcc catgggcgac accgggcgca cggccagcag tcattatctc gatctggagg
     4381 tggtgcggcg gcggctggaa cagaaattca aactggtgcc cgcggtcatg ccgggcggcg
     4441 gaccagcgcg gtacgtgggc gttgccggga ccaatctgcg catcggattc atcacgccga
     4501 tctcgcgtca cttttgcgaa acatgcaatc gcgtgcgcct gtcggccgat ggcgcgcttt
     4561 atctctgcct cggtcaggat gacaaggtgg aattacggcc gttgctgcgc gcgggtatca
     4621 gcgacgagga tttgaaggat acgatccgcg ccgccatcgc gcgcaaaccg gaaagacatg
     4681 agttccggga acagccgcaa aaaatcgtcc ggttcatgtc ccgaactggg ggttaaaaat
     4741 tagacaggat taacaggatt tttcaggatt tacaggatta aagattttaa aaattaaatt
     4801 tttaaatttt aaatttaaaa tttttaaatc ctgttaatcc tgtttttaat cctgttaatc
     4861 ctgtgaaatt tttttcagaa gagcttagtg aagctgtcgg aggagcttgt cttcgagctc
     4921 gatacgggcc gcaagctcct cgccgaggcg ggaaagatcg tcgctgaatg acgcggcgat
     4981 ctcgcagtga tctccgcaat cgtacttgtc gttgaaatcc agtgccgctt cggtggattt
     5041 ggcgatgcgg gggtaaagat tctcggccag cgcggaaatc tgctggcggc gttcggtgcc
     5101 gttgacgatg cgttcgtaaa ggctgaaatg cccggcggcg atgtaatcaa ccaacacctg
     5161 gcaaaatttc tggagcaatt cctgcgagtg cttgacgcgc ggattcttgc cgttgccgta
     5221 tggctccagt cccgccagct ggcaatacag ggacagcatc tcggtacgct ccgtgaccag
     5281 cttctgcacc agctcctggc tcccggtgcg gcgctccttg gccctgtttt cctgtggtgt
     5341 catctctctc ttgtgctccg ttgtcatgat cccgccttcc gtttgttctg agtgggtggc
     5401 gaaactatac gccctgacaa tgctgttgtt aacagcgcca atgccgacaa taaccgctga
     5461 tgacaggaaa atgaaaataa tttcagccgg gcatccccgg caggcggccc ttgacatcgc
     5521 gcaacatacg ttgcagtccg cccttgccca tgcgtttcat caccttctgc atctgatcga
     5581 attgcttcag caggcggttg acgtcctgca cctgcgtgcc cgagccggcg gcgatgcgcc
     5641 gcttgcgcga accgcggatc gtgtccgggt ggttgcgttc ctgcggcgtc atggaattaa
     5701 tgatagccac caatcgcctg acctgccggt cgcccaattg tgacttggcg acctcgctga
     5761 cttcgccaac gcccgggagc ttttccatga gactggcgag cccgcccatc ttttccatct
     5821 gcaataattg gtcgcgaaaa tcctcaagat cgaagccctt gcccttgacg agtttctggg
     5881 cgaattcctg tgccttctcc ttgtcggcgg cctgggttgc ctgctccacc aggcccagca
     5941 cgtcgcccat gccgaggatg cgcgcggcca cgcgatcagg ataaaacggc tccagcgcgg
     6001 cgggcttctc gcctacgccc atgaacttga tcggcttgcc ggtcacctga cggatcgaga
     6061 gcgccgcgcc gccgcgcgca tcgccatcgg tcttggtcag gatcacgccg gtgagcggca
     6121 aggcttcgtt gaatgccttg gcggtattca ccgcgtcctg accggtcatg ctgtccacca
     6181 cgaacagggt ctcgaccggc tccagcgccg cgtgcacggc gcggatctcc tgcatcatcg
     6241 gctgatccac gtgcaggcgg ccggcgctgt ccacgatcag cacgtccatc aaccgcttgc
     6301 gcgcctcttc caccgcctgg cgcgcgatgt ccaccggctt ctggtcggga cggctcggga
     6361 agaattccgc gcccacggca gcggccagtt tctgcaactg atcgatcgct gccggacgat
     6421 agacgtcggc gctcaccacc atgacggatt tcttctcgcg ctcacgcagg aaacgcgcga
     6481 gcttggcgac ggtcgtggtt ttgccggaac cctggagccc cgccaccagg atcaccgccg
     6541 gcggacg
//
LOCUS       DBNGZE010000307         2576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1776597, whole genome shotgun sequence.
ACCESSION   DBNGZE010000307 DBNGZE010000000
VERSION     DBNGZE010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1776597"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="AC16FV_08025"
     CDS             <1..880
                     /locus_tag="AC16FV_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR2850038.1"
                     /translation="VTPIMTPPSPASLNTRTKLRIARTLYWTLTGLRRIMGLTDNAQV
                     ARRGIRWQLDLREGIDLAIYLFGYFEYETVRAYRRLLKPGDTVLDIGANIGAHTMHLA
                     RCVSPTGKVIAFEPTAYAYNKLRRNVSLNPPLAGLIQAEQIMLMSSEETQAKPRLYSS
                     WQIHGAVAGTHPKHGGRLMDTTGARNTTLDRFIAEHKPGVISLIKMDVDGHECQVLRG
                     AHELLRRDKPILLMEIMPYGLDEAGASLDELLEILSAHGYRLYELDGLTALPIDNSIR
                     QKIPPAGGINVLCYAK"
     gene            complement(907..2532)
                     /locus_tag="AC16FV_08030"
     CDS             complement(907..2532)
                     /locus_tag="AC16FV_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR2850039.1"
                     /translation="MRNKAVNISKRNIIAYAGLGSILVLTLFLRLYGIDWGLPTSLHP
                     HYSYHPDEVPLLVWAQWLSQGQIISKQFIYGGTFYFLILRACMYFGDMFGELIGGFNA
                     LANAILVARYLQVFLALLTVLLVYECGRLLYDRRTGLIAALVLAVAPAHIVATQTVRP
                     DAISAFLVILITLTAAKLLKSASSGRRKLLVYSGIAIGAAAAFRLPLIAFGLLPVFAY
                     IVARQRADGSSFRELVLDWDALWFTVTIVVTYIVLSPHILVYPEAFVMGIKVTTSYET
                     VVFPDATDRGPVFFQYAWRLLHQALGYPGYFLALGGVIYALMRRHTEDIIVLAGIGLY
                     FIMLASVTLTFVRYTLPMLPLLALLSGVVAVQLSERVHRARKIIYVVAACLFAWTLAA
                     DIALLNVTASKNSRVQAREWVAQNIPQGKSTLLVKQYDGDDFFNPVITSKHNNMAVLL
                     TDGSDSRRLFTEIKFDDVILHELFYRDMERLGSRHPRKEVWDFYEAMTGAGLKLIKEI
                     KVPVQFLGIDFSGSFEAIDFSVINPGIRIYQAP"
BASE COUNT          674 a          746 c          641 g          515 t
ORIGIN      
        1 agtcacaccg ataatgaccc ccccatcgcc ggccagcctg aatacccgga caaagctgcg
       61 cattgcgcgc acgctctatt ggacgctgac cggcttgcga cgcatcatgg gcctcacgga
      121 caatgcccag gtggcccggc gcgggatccg ctggcagctg gaccttcggg aaggaatcga
      181 tctcgcaatc tatcttttcg gatattttga atacgaaaca gtccgcgctt atcgtcgact
      241 gttgaaaccg ggggataccg ttctcgacat cggcgccaac atcggcgccc acaccatgca
      301 cctggcccgc tgcgtttcgc cgaccggcaa ggtcatcgcc ttcgagccca ccgcctatgc
      361 ctacaacaaa ctgcggcgga acgtttcact taacccacca ttggccggcc tgattcaggc
      421 cgagcagatc atgctgatgt cctcggagga gacacaggcc aagccacggc tctactcaag
      481 ctggcaaatt catggcgccg ttgccggcac ccacccgaag cacggcggac gcctgatgga
      541 taccaccggc gcgcgcaata cgacgctgga ccgcttcatc gccgaacaca aacccggcgt
      601 catatccctc atcaagatgg acgtggacgg acatgaatgc caggttctgc gcggcgcgca
      661 cgagctactc cggcgcgata agccgattct gctgatggaa atcatgccgt atggcctcga
      721 tgaagcgggt gcttccctgg acgagcttct ggaaatcctg tccgcgcatg gatacaggct
      781 ttacgagctg gatgggctaa ctgccctgcc cattgacaac agcatccggc aaaaaatccc
      841 gccggccggt ggaatcaatg ttctttgtta cgcaaagtaa tctgtttacc cgtggctgca
      901 ggagatctac ggcgcctggt agatacgtat tcccggattg atgaccgaaa agtcgatagc
      961 ctcgaatgaa cccgaaaaat ctattccgag aaactgcacc ggcactttaa tctccttgat
     1021 gagcttcaag cctgcgcctg tcattgcctc gtagaaatcc caaacctcct tcctgggatg
     1081 ccggctgccg agcctttcca tgtcgcgata aaacaattca tgcaggatca catcgtcaaa
     1141 ttttatttcg gtgaaaagcc tccggctatc gcttccgtct gtcagcaaaa cagccatatt
     1201 gttgtgcttt gaagttataa ccggattgaa aaaatcgtcg ccatcatatt gcttcacaag
     1261 cagtgttgat tttccttgcg gaatattctg cgctacccac tcacgggcct gcacacgcga
     1321 attctttgat gcagtcacat taaggagggc aatatcggcc gccaatgtcc aggcgaagag
     1381 acaagccgcg acaacatata ttattttgcg ggcacgatgt acgcgctcgg aaagctggac
     1441 ggcaacaaca ccgctcaaca acgccagtaa cggaagcatc ggcaatgtat aacggacgaa
     1501 tgtcaacgtt accgatgcaa gcatgatgaa gtacagccca atacctgcca atacaattat
     1561 atcttcggtg tgacgacgca tcagggcgta gatcacacca cccaaagcaa gaaagtagcc
     1621 cggataacca agggcctgat gcagcaggcg ccatgcatac tggaaaaata ccgggccacg
     1681 atccgtggcg tcgggaaaaa caacggtctc gtagctcgtt gtaaccttaa tacccataac
     1741 aaatgcttcc ggatacacca gaatatgcgg gctcagcaca atataagtca ccacgatagt
     1801 gacggtgaac catagcgcat cccaatcaag caccaattcc cggaatgaac tcccatcggc
     1861 tcgttgccgc gcaacgatat aggcaaacac cggcagaaga ccgaaggcga tcagtggcaa
     1921 tcgaaacgcc gccgctgcac cgatggcaat accggaatat acgagaagct tcctgcgccc
     1981 ggatgacgcc gatttcagca atttggcggc cgtcaacgtg atcaatatca ccagaaacgc
     2041 actgatggcg tcgggacgga cggtctgcgt cgcaacaata tgtgccggcg ccacggccag
     2101 aaccagcgcg gcaatcagtc cagtcctccg gtcatataac aagcggccac actcgtagac
     2161 cagcagtacg gtcaataatg ccagaaaaac ctgcaggtac ctggcgacca ggatggcgtt
     2221 agccagcgca ttgaagccgc caatgagctc tccgaacatg tcgccgaaat acatgcatgc
     2281 gcggagtatc agaaaataaa atgtcccgcc gtagataaac tgcttgctga tgatctggcc
     2341 ctgggaaagc cattgcgccc aaacaagcaa tggaacttca tccgggtgat aggagtaatg
     2401 tggatgaagc gaagttggca atccccaatc gatgccatac aacctgagaa acagcgtaag
     2461 caccagaata ctgccaagac cggcataggc gatgatgttc cgtttgctaa tatttaccgc
     2521 cttattcctc attgcaccgc ccggaagttg aattgataat ctttgctata cttttc
//
LOCUS       DBNGZE010000308         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_43559, whole genome shotgun sequence.
ACCESSION   DBNGZE010000308 DBNGZE010000000
VERSION     DBNGZE010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_43559"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="AC16FV_08035"
     CDS             complement(<1..355)
                     /locus_tag="AC16FV_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850040.1"
                     /translation="MCLTLFQIHSPFSYTAGGDSAPPDRTVRRSALRIFELTSGGLAR
                     IGEIRSSARENLEKQEQKGRKLYRPTPAGSMEPVGKRGFLGESGKKRLSGNGFYSLGR
                     RARTAKQPRVFAHGIA"
     gene            329..>3859
                     /gene="gltB"
                     /locus_tag="AC16FV_08040"
     CDS             329..>3859
                     /gene="gltB"
                     /locus_tag="AC16FV_08040"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR2850041.1"
                     /translation="MNLEQRKTQQGLYDPATEHDACGVGFVVDMKGRRSHKIVTQALT
                     VLRNLMHRGACGCEENTGDGAGILIQMPHAFLNRECGKLGIQLPEAGHYGTGMVFLPR
                     DGAEARRCQEQFAAIVREEGQTLLGWRDVPVDHSSLGPTACSAEPGFKQIFIGRAAGI
                     TDDMSFERKLYVIRKRIEHAIRNSDLKERALFYIPSLSARTLVYKGMLTPTQVEPMFP
                     DLADPAVESALALVHSRFSTNTFPSWPLAHPYRYIAHNGEINTLRGNINWMRAREALC
                     ASPLFGDDLKKILPIVMEGGSDSATFDNVLEFLYMAGRELPHAVLMMIPEAWSGHEMM
                     DENRKAFYEYHGCLMEPWDGPASIAFTDGTVIGAILDRNGLRPSRYYVTKDGLVVMAS
                     EVGVLDIPPENVLIKERLHPGRIFLVDTRAGRIIDDAELKQRYVGAQPYKEWLKQNLV
                     PLDQLPRPPQLPEPDHATVLQRQQAFGYTHEDLRILMAPMAQKGEEPVGSMGNDAALA
                     VLSDKPRLLYDYFKQLFAQVTNPPLDAIREELVTQIETPLGPERNLLQPGPESCRLLK
                     LKTPILSNEELAKIRHLDRPGFKTVTLPMLFPAGSDGQGLARALDELCRQASDAIAQG
                     ATFIILSDRGVDAHHMPIPTLLATAGVHHHLVREGTRVKVGLIVESGEPRETHHMALL
                     LGYGAGAINPYLAFETLDDMIRQNLLNGVDHAQAVKNYIKACNKGVVKVISKMGISTI
                     QSYRGAQIFEAIGLDKKFVDQYFTWTASRVGGIGIDIVAQETSLRHHRAYPDRPVGPP
                     ELDWGGHYQWRRDGEYHMLNPDTVAKLQHATRANSYERFKEFSRACDDGAQHLATLRG
                     LMELKLADRPIPIEEVEPVESIMQRFATGAMSYGSISLEAHEALAIAMNQIGGRSNCG
                     EGGEDPERYGTNRNSRIKQVASGRFGVTSHYLVNCTDLQIKMAQGAKPGEGGQLPGHK
                     VYPWIAKVRYSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANREARIHVKLVSEIGV
                     GTVAAGVAKAHADVVLISGHDGGTGASPLSSLKHAGLPWELGVAETQQVLVMNKLRDR
                     IVVQTDGQMKTGRDVVIAALLGAEEYGFSIAPLVVLGCIMMRVCHLNTCPVGIATQDP
                     ELRKKFAGNPEHVVNFFRFVAQETRELMAQL"
BASE COUNT          714 a         1246 c         1219 g          680 t
ORIGIN      
        1 ccgctatgcc atgcgcgaac acacgcggct gtttggccgt tctggcgcgc cggcctagcg
       61 aataaaagcc gtttccgctc aaccgcttct ttcccgattc gcccaaaaag ccccgtttcc
      121 caacgggttc cattgaaccg gccggggtgg gtcggtataa ttttcgcccc ttttgctcct
      181 gtttttcgag gttttcgcgg gcagaagaac gaatttcccc gatccgggcc aagcccccgc
      241 tggtgagttc gaaaatacga agggctgatc ggcgcaccgt cctgtccggt ggtgccgaat
      301 cgccgccagc ggtatatgaa aacggtgaat gaatttggaa cagcgtaaga cacaacaggg
      361 tctctatgat ccggccaccg agcacgacgc ctgcggcgtc ggcttcgtgg tggacatgaa
      421 aggccgtcgt tcgcacaaga ttgtcacgca ggcattgacg gttctgcgca acctcatgca
      481 tcgcggcgcc tgcggttgcg aggagaacac cggcgacggc gccggcatcc tgatccagat
      541 gccgcatgcc ttcctcaatc gcgagtgcgg caagctcggc atccaactcc ccgaggccgg
      601 tcactacggc accggcatgg tgtttctgcc gcgcgatggc gccgaggcgc gccgctgtca
      661 ggaacagttc gccgccatcg tgcgcgaaga ggggcagacc ctgctcggct ggcgcgatgt
      721 cccggtggat cattcctcgc tcggcccgac ggcgtgttcg gccgagcccg gcttcaagca
      781 gattttcatc ggccgcgccg ccggcatcac cgacgacatg agcttcgagc gcaagctcta
      841 cgtgatacgc aagcgcatcg aacacgcgat ccggaattcg gacctgaaag aacgcgcgct
      901 gttctacatt ccgagtctgt cggcgcgtac gctggtctac aagggcatgc tcacgccgac
      961 ccaggtggag ccgatgttcc ccgacctggc cgatccggcg gtggaatcgg cgctggcgct
     1021 ggtgcattcg cgcttctcga ccaatacctt tccgtcctgg ccgctggcgc acccgtaccg
     1081 ttacatcgcc cataatggtg agatcaacac cctgcgcggc aacatcaact ggatgcgcgc
     1141 gcgcgaggcg ctgtgcgcct cgcccctgtt cggcgacgac ctgaagaaga ttcttcccat
     1201 cgtgatggag ggcggttcgg actcggccac cttcgacaat gtgctggaat tcctgtacat
     1261 ggccgggcgc gagctgccgc acgccgtgtt gatgatgatt ccggaggcgt ggagcgggca
     1321 tgagatgatg gacgagaacc gcaaggcctt ctacgaatac cacggctgcc tgatggagcc
     1381 gtgggacggc ccggcctcga ttgccttcac cgacggtacg gtcatcggcg cgattctcga
     1441 ccgcaacggc ctgcgcccgt cgcgttatta cgtcacgaag gacggcctcg tggtcatggc
     1501 ctccgaggtc ggcgtactcg acatcccgcc ggagaacgtg ttgatcaagg agcgcctgca
     1561 tccgggccgc atcttcctgg tggacacgcg cgcgggccgc atcatcgacg acgctgaact
     1621 taagcagcgc tatgtcggcg cgcagcccta taaagagtgg ctgaaacaga atctggtccc
     1681 gctcgatcaa ttgccgcggc cgccgcagct gccggagccg gatcacgcca ccgtcctgca
     1741 acggcagcag gccttcggtt acacccacga ggacctgcgc atcctgatgg cgccgatggc
     1801 gcagaagggt gaggaaccgg tgggttccat gggcaacgac gccgcgctgg cggtgctgtc
     1861 cgacaagccg cgcctgctct acgactattt caagcagctg ttcgcgcagg tgaccaatcc
     1921 gccgctcgac gccatccgcg aggagctggt gacgcagatt gaaaccccgc tcggtcccga
     1981 gcgcaacctg ctccagccgg ggccagagtc ctgccgcctg ctcaagctca agacgccgat
     2041 tctgagcaac gaggagttgg cgaagatacg ccatctcgat cgccccggtt tcaagaccgt
     2101 gacgctgccc atgctgttcc cggccggttc ggacgggcag gggctggcgc gggccttgga
     2161 cgagctgtgc cgccaggcca gtgacgccat cgcccagggc gccactttta ttatcctctc
     2221 ggaccggggc gtggatgcgc atcacatgcc gattccgacg ctgctggcca cggccggcgt
     2281 gcatcatcat ctggtgcgcg aaggcacacg tgtgaaggtc gggctcatcg tcgaatccgg
     2341 cgagccgcgc gagacgcacc acatggcgct gctgttgggt tacggcgccg gcgcaattaa
     2401 tccatacctg gcatttgaaa cgctcgacga catgatccgc cagaatctcc tgaatggcgt
     2461 ggatcacgcg caggcggtca agaactacat caaggcgtgt aacaagggcg tggtgaaggt
     2521 gatttccaag atgggcattt ccaccatcca gtcctatcgc ggcgcgcaga ttttcgaggc
     2581 cattggtctc gacaagaaat tcgtcgacca gtatttcacc tggaccgcct cgcgtgtcgg
     2641 cggcatcggc atcgacatcg tggcgcagga aacgagcctg cgccatcatc gtgcttaccc
     2701 cgaccggccg gtgggcccgc ccgagctcga ctggggcgga cactaccagt ggcgccgcga
     2761 tggcgagtat cacatgctca atccggacac tgtggccaag ctccagcatg ccacgcgcgc
     2821 caactcttat gagaggttca aggaattttc gcgcgcctgc gacgatggcg cgcaacacct
     2881 ggcgacgctg cgcgggttga tggaactgaa actcgccgac cggcctatcc cgatcgagga
     2941 ggtcgagccg gtggaatcca tcatgcagcg tttcgccacc ggcgccatgt cgtacggctc
     3001 gatcagtctg gaggcgcacg aggcgctggc gatcgccatg aaccagatag gcggacgctc
     3061 caactgcggc gaaggcggcg aagacccgga gcgttacggc acaaacagga actcgcgcat
     3121 caagcaggtg gcctcgggcc ggttcggcgt caccagccac tacctcgtga actgcaccga
     3181 cctgcagatc aagatggcgc agggcgccaa gcctggcgag ggcggacagc tgcccggcca
     3241 caaggtttat ccatggatcg ccaaggtgcg ctactcgacg cccggcgtgg ggctgatctc
     3301 gccgccgccg catcacgaca tctattcgat cgaggacctg gcgcagctca tccacgacct
     3361 caagaacgcc aaccgcgagg cgcgcatcca cgtgaagctc gtctccgaga tcggtgtggg
     3421 cacggtcgcc gccggcgtgg ccaaggcgca cgccgacgtg gtgctcatct ccggtcatga
     3481 cggtggtacc ggcgcgagcc cgctgtcctc gctcaagcac gccggcctgc cgtgggaact
     3541 cggcgtggcc gagacccagc aggtgctggt gatgaacaag ctgcgcgacc gcatcgtggt
     3601 gcagaccgac ggccagatga agaccggccg cgacgtcgtg atcgcggcgc tgcttggcgc
     3661 ggaggagtac ggcttctcga tcgcgccgct ggtggtgctg ggctgcatca tgatgcgcgt
     3721 atgccatctg aacacctgcc cggtcggcat cgccacccag gatccggagc tgcgcaagaa
     3781 attcgccggc aacccggagc acgtggtgaa tttcttccgc ttcgtggcgc aggagacgcg
     3841 cgaactgatg gcgcagctc
//
LOCUS       DBNGZE010000309         2522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_667771, whole genome shotgun sequence.
ACCESSION   DBNGZE010000309 DBNGZE010000000
VERSION     DBNGZE010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_667771"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC16FV_08045"
     CDS             complement(<1..505)
                     /locus_tag="AC16FV_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HIR2850042.1"
                     /translation="MSQATRTRTSAPLTPRILRLLREAALYILGAVAIYLLISLWTYS
                     TSDPSWSHRGTNTIVFNTGGRAGAWLSDVLFNLFGYVAYLLPVMIGFSGWRIYQHRKD
                     PAPVGVKHRLLMTGSFFAAVIGACGLAGLHFAQSMTGMPFASGGWLGNAVGLGLASTF
                     SFTGATLF"
     gene            complement(577..1647)
                     /gene="ald"
                     /locus_tag="AC16FV_08050"
     CDS             complement(577..1647)
                     /gene="ald"
                     /locus_tag="AC16FV_08050"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR2850043.1"
                     /translation="MRIGIPKEIKVHEGRVALIPPAVNELVRNGHEVFVQSSAGVASG
                     YPDQDYRQAGAQIVPDATALYGSAQMVLKVKEPIAPEYDLLRKDHILFSYLHLAATPK
                     LAQVLVKKGLTAVGFETVVANGKLPLLAPMSAIAGRLAVHIGTTLLHGPAGGRGVMLG
                     GLPSTERGHVVILGAGAAGGNAAAVAAALGAQVTVFAPHREELERMHALGPNVTALPS
                     YAPLIAQAVSGADLLIGAVLIPGAKAPKLVSAELVRRMKPGSVIIDISVDQGGCIETT
                     RPTDYDHPTYIQDGVVHFGVTNMPGAVPRTASQALSTVLTPYVLRLATEGGLNDPAIA
                     AGVNVSGGRIVHLAVAAALASN"
     gene            1787..>2522
                     /locus_tag="AC16FV_08055"
     CDS             1787..>2522
                     /locus_tag="AC16FV_08055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2850044.1"
                     /translation="MTDKVKHCRLLILGSGPAGYTAAVYAARANLNPVLITGLEQGGQ
                     LMTTTDVDNWPGDIEGLQGPALMERMQKHAERFKTEIIFDHIHTADLKSRPFRLTGDS
                     GVYTCDALIIATGASAKYLGIPSETALKGKGVSGCATCDGFFYKGQKVAVIGGGNTAV
                     EEAIYLSNIASHVTVVHRRDKFKAEAILVDQLMAKTNHGNISVEWNHEVDEILGDKNG
                     VTGVRIKNNNGKTKQLDVTGVFIAIGH"
BASE COUNT          521 a          814 c          737 g          450 t
ORIGIN      
        1 ggaacagcgt cgcgccggtg aagctgaagg tcgatgccag accgagcccc acggcgttgc
       61 cgagccagcc gccgctggca aacggcatgc cggtcatgct ctgcgcaaag tgcaggccgg
      121 ccagcccgca ggcgccgatc acggcggcaa aaaagctgcc ggtcatcagc agccggtgct
      181 tcacgcccac cggggcggga tccttgcggt gttggtaaat gcgccagccg gaaaatccga
      241 tcatgactgg caggaggtag gcgacgtaac cgaagaggtt gaacagcacg tcggacaacc
      301 acgcccccgc ccgtccgccg gtattgaaga cgatggtgtt cgtgccccgg tgcgaccagg
      361 acgggtccga agtggaatag gtccacagcg atatcagcag atagatagcg acagccccga
      421 ggatgtagag ggccgcttca cgcaacaggc ggagaatacg cggtgtcagc ggtgcgctgg
      481 tcctggtccg ggtagcttgg ctcacaacct tccttagaat gccgtgtaat tggcggattt
      541 tcccattttt ccgcgctccg cgccaagtat ttaggcttaa ttactcgcga gcgccgcggc
      601 caccgccaga tgcacgatcc tgccgccgct gacgttcacg cccgcggcaa tcgccggatc
      661 gttcaatccg ccttccgtcg ccaagcgcaa cacgtacggc gtaagcaccg ttgacagagc
      721 ctgcgacgcc gtgcgcggca ccgcgcccgg catgttggtc acgccgaaat gcaccacgcc
      781 atcctgtata taagtgggat gatcgtaatc cgtcggccgc gtggtctcga tgcaaccgcc
      841 ctggtcaacc gagatgtcaa taatcacgct gcctggtttc atccggcgca ccagctccgc
      901 gctcacgagc ttgggtgcct tcgcgcccgg gatcagcacc gcaccgatca gcagatcggc
      961 gccggaaaca gcttgcgcga tcagcggcgc ataagatggc agcgccgtca cgttcggacc
     1021 aagcgcgtgc atgcgttcca gctcctcgcg gtgcggggcg aatacggtta cctgcgcgcc
     1081 gagggcggcg gcgaccgcgg cggcattgcc accagccgcg cccgcgccaa gaatcaccac
     1141 atgaccgcgc tccgtggacg gcagaccgcc cagcatcacg cctcttccac ccgcgggacc
     1201 gtgcagcagg gtggtgccga tgtgtaccgc caggcgtccg gcgatagcgc tcatgggcgc
     1261 gagcagcggc aatttgccat tggccacgac cgtttcaaat cccactgccg tcaggccctt
     1321 tttcaccaac acctgcgcca gtttgggcgt ggccgccaga tgcagatagg aaaacaggat
     1381 atggtccttg cgcagcagat catattccgg ggcaatcggt tccttgacct tgagcaccat
     1441 ctgcgcgctg ccatacagcg ccgtggcatc cgggacaatc tgcgcgcccg cctgacggta
     1501 atcctggtcc ggatacccgc tcgctacacc cgcgctcgac tggacgaaga cctcatgacc
     1561 gtttcgcacc aactcattca cggcgggcgg gatcagggcc acgcgccctt catggacttt
     1621 tatttctttt ggaataccga tacgcataca gtctccttta ccgcctttgc gtgcatgtcg
     1681 taacatacgc gtggaaatcg gcactaccac tgccggcgca gcacgataag aacttccacg
     1741 gtgtttgtct atcaaatgtt ttcttgtttc tcaacggagt caccgcatga cggataaggt
     1801 caaacactgc cggttgctga tcctcggctc cggccccgcc ggttacacgg ccgcggttta
     1861 cgccgcgcgc gccaacctga atccggtcct catcaccggc ctcgaacagg gtggtcagct
     1921 catgaccacc accgacgtgg acaactggcc gggcgacatc gagggcctgc aaggcccagc
     1981 gctgatggaa cgcatgcaga agcacgccga gcgtttcaag accgagatca tcttcgacca
     2041 tatccatacg gccgatctca agtcgcgacc gttcaggctc acgggcgatt ccggcgttta
     2101 cacctgcgac gcgctaatta tcgccaccgg cgcctccgcc aaatatctgg gcatcccctc
     2161 ggaaacggca ctaaagggca agggtgtgtc cggctgcgcc acttgcgacg gattctttta
     2221 taaaggtcag aaagtggccg tcatcggcgg cggcaacacc gccgtggaag aggcgattta
     2281 tctgtccaac atcgcctcgc acgtcaccgt ggtgcaccgg cgcgacaaat tcaaggccga
     2341 ggcgattctg gttgatcagc tcatggcgaa aaccaatcac ggcaacattt ccgtcgaatg
     2401 gaaccatgaa gtggacgaga ttctgggaga caagaacggc gtcaccggcg tgcgcatcaa
     2461 gaacaacaac ggtaagacaa aacagctcga cgtgacaggc gtgttcatcg ccatcggtca
     2521 cg
//
LOCUS       DBNGZE010000310         2849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_877391, whole genome shotgun sequence.
ACCESSION   DBNGZE010000310 DBNGZE010000000
VERSION     DBNGZE010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_877391"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC16FV_08060"
     CDS             <1..358
                     /locus_tag="AC16FV_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2850045.1"
                     /translation="DLVLEGMAQLIATTLRTTDIVARYGGEEILIIAPSTPVKTAANL
                     AERLRKVVEDASFEVPVELNRDISALGVTVSIGVACFSQHTKDMQDLIQDVDKAMYRA
                     KKEGRNRVVTACTTDA"
     gene            complement(372..1484)
                     /locus_tag="AC16FV_08065"
     CDS             complement(372..1484)
                     /locus_tag="AC16FV_08065"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR2850046.1"
                     /translation="MATCASYRLLRPLLFRLPAETSHHLTFSLLRLFYRLPGAGVMTR
                     ALFTRQPLALPVTVMGLGFPNPVGLAAGLDKNAEYIRPLADFGFGFLELGTVTPRPQP
                     GNPKPRMFRLPRQAALINRMGFNNAGIGRFLVNLARAGRTCPLGINIGKNRDTPLERA
                     VEDYLSALRTAYPQADYVTINISSPNTAGLRDLQNEENLEELLRALKTEQTALVERSG
                     RYVPIALKIAPDLDDAQIAAIARLVLEHKFDAIIATNTTITRPGLANDPLASQNGGLS
                     GRPLKPLSTEIIRKLYGHLQGKIPIIGVGGIENADDAWDKLVAGADLIQIYTALIYEG
                     PCVAQAIVRGLAERVRASGCATLTEAVAQARRQKSA"
     gene            complement(1539..>2849)
                     /locus_tag="AC16FV_08070"
     CDS             complement(1539..>2849)
                     /locus_tag="AC16FV_08070"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR2850047.1"
                     /translation="GNADRANADTEGRLTVPPDDARYTLKRVWLTREEEQGYYYGFSN
                     EGLWPLCHLVHERPVFRAGDWEHYRRANRRFADAVLDEAGSGKAVVLVQDYQLALVPR
                     MLKEARPDLRVGLFWHIPWPNPEAFRICPWGAEILDGMLGADLLGFHLQQYCNSFLET
                     VDRVLESRLDWDHFSVELRGIRTRVRPFPISVQPWSERHVPMDEALARQIAELKTQHQ
                     LGDAFIAVGVDRIDYTKGLPERFRAIAQLLEKYPQYRGRFTFVELGAPSRTHIPRYRE
                     HLDMIEALADEINWKYQSEGWKPIHFLVAHHDARTVHAFLRLAPMCIVSSLHDGMNLV
                     AKEYVAAQSGGDGVLVLSEFAGAARELGDALIVNPYDTEQFAEAIRYGLEMDTNERRA
                     RMARLGRQVEEHNIYRWAANFLSALAAERAAGVEATSHDKAASS"
BASE COUNT          528 a          860 c          925 g          536 t
ORIGIN      
        1 tgacctggtg ctggaaggca tggcgcaact catcgccacc accctgcgga ccacggacat
       61 cgtggcgcgc tatggcgggg aggaaatctt gatcatcgcg ccgagcacgc ccgtgaagac
      121 ggcggccaat ctggccgaga ggctgcgcaa ggtcgtggaa gatgcctcgt ttgaggtgcc
      181 ggtcgagctg aatcgcgata tcagcgcgct gggcgttacg gtgagtatcg gtgtggcctg
      241 tttcagccag cacaccaagg atatgcagga tctgatacag gacgtcgaca aggcgatgta
      301 ccgcgcgaaa aaagaagggc gcaatcgcgt ggtcacggcc tgcaccacgg acgcctgatt
      361 tcgcgtccca atcaggccga cttttgccgg cgcgcttgcg cgacagcttc tgtcagcgtc
      421 gcgcagccgg atgcccgcac gcgttctgcc aggccgcgca caatggcctg cgccacacac
      481 ggcccttcat aaatcagcgc ggtataaatc tggatcagat cggcgccggc aacgagtttg
      541 tcccaggcat cgtccgcgtt ctcgatcccg cccacaccga tgatcgggat ctttccctgg
      601 agatggccgt acagcttgcg gatgatttcc gtggaaagtg gcttgagcgg gcggccactc
      661 aggccgccgt tctgactcgc cagcgggtcg ttcgcaaggc cgggccgggt aatggtggtg
      721 ttggtcgcaa tgatcgcatc gaatttgtgt tccagcacca gccgcgcgat cgcggcaatc
      781 tgggcatcgt ccagatcggg cgcgatcttg agggcaatgg ggacgtaacg tccggacctt
      841 tcgacgagcg cggtctgttc cgttttcagt gcgcgcagca attcttcgag gttttcttca
      901 ttctgaaggt cgcgcagacc ggcggtattc ggcgaggaaa tattgatggt gacataatcg
      961 gcttgcggat acgccgtacg cagcgcgctg agataatctt ccaccgcgcg ttccagcggt
     1021 gtgtcgcggt tcttgccgat gttgattccg agcgggcagg tcctccccgc gcgcgcgaga
     1081 ttgacaagga accggccgat gccggcgttg ttgaatccca tgcggttgat cagcgctgcc
     1141 tgacgcggca gccggaacat gcgcggtttc ggattgccgg gctgcggccg gggcgtgacg
     1201 gtaccaagct cgagaaagcc aaagccgaaa tccgccagcg gcctgatgta ttcggcgttt
     1261 ttatccagac cagccgccag tcccaccggg ttgggaaaac ccagccccat cacggtgaca
     1321 ggcaatgcca gcggctgtcg tgtgaacaag gcgcgcgtca tgacgcccgc accgggcaag
     1381 cggtagaaca gacgcaacaa cgagaatgtc agatggtggc tggtttcggc cgggagccgg
     1441 aaaagcagcg gacgcaacaa cctgtacgag gcgcaggtgg ccatgagggt caggcatcat
     1501 aacccaattc cggttttgaa atgaacgatg aacgtccgct atgagctggc cgccttgtca
     1561 tggctggttg cctcgacgcc cgccgcgcgt tcggcggcga gggccgagag aaaattcgcg
     1621 gcccagcggt atatattgtg ttcctcgact tggcggccga ggcgcgccat gcgcgcacgt
     1681 cgttcattgg tatccatttc gaggccgtag cgaatggctt cggcgaactg ctcggtgtcg
     1741 taagggttga cgattagcgc gtcgcccagc tcgcgcgccg cgccggcgaa ctccgagagc
     1801 actagcacgc catcgccgcc ggattgcgcc gctacatact ccttcgccac cagattcatg
     1861 ccatcatgca gggagctcac gatgcacatc ggcgccaggc gcagaaaggc gtgcacggtc
     1921 ctggcatcgt ggtgcgcgac caggaagtga atcggtttcc atccctcgct ctggtacttc
     1981 cagttgattt cgtccgccag cgcctcgatc atgtccaggt gctcgcgata gcgggggata
     2041 tgcgtgcgcg agggcgcgcc gagttcgacg aatgtgaagc gcccgcggta ctgcgggtat
     2101 ttttcgagca attgcgcgat ggcccggaaa cgttccggca ggcccttggt gtagtcgatg
     2161 cgatccacgc caactgctat gaaagcatcc cccagttgat gttgcgtctt gagttcggcg
     2221 atctgtcgtg cgagcgcttc gtccatgggc acgtggcgct cggaccatgg ttgtacgctg
     2281 atcgggaacg gccgcacacg cgtgcggatc ccgcgcagtt ccacggaaaa gtgatcccag
     2341 tcgagccttg attcgagcac gcggtccacg gtctcgagga agctgttgca gtattgctgc
     2401 aggtgaaagc cgaggagatc ggcgccgagc atgccgtcga gaatctccgc accccagggg
     2461 cagatacgga acgcctccgg gttgggccag gggatgtgcc agaacaatcc gacgcgcaaa
     2521 tcggggcgtg cctccttgag catccgcggc acgagcgcga gctggtaatc ctgtaccagc
     2581 accacggcct tgcccgatcc cgcctcgtcc agcaccgcat ccgcgaagcg gcggttggcg
     2641 cggcgatagt gttcccagtc cccggcgcgg aagacgggac gctcgtgcac caggtggcac
     2701 aacggccaca ggccctcgtt cgagaagccg tagtaataac cctgttcctc ctcgcgcgtg
     2761 agccacacgc gcttgagcgt gtagcgcgcg tcgtccggcg gcaccgtgag ccggccctcg
     2821 gtatcggcat tggcgcgatc ggcgttgcc
//
LOCUS       DBNGZE010000311         7211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1293886, whole genome shotgun sequence.
ACCESSION   DBNGZE010000311 DBNGZE010000000
VERSION     DBNGZE010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1293886"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC16FV_08075"
     CDS             <1..305
                     /locus_tag="AC16FV_08075"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: protein motif:HMM:NF014849.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR2850048.1"
                     /translation="VGCWPRSGILWTGPVRTPESLLQLVQTLNAALTPCGYEPETRPY
                     AAHLTLARKVRRCHHRHPIEPLAWEVRQFCLVESHTHSDGARYEILRSWPLNSPAA"
     gene            complement(286..423)
                     /locus_tag="AC16FV_08080"
     CDS             complement(286..423)
                     /locus_tag="AC16FV_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850049.1"
                     /translation="MAQRSGRNALRILADEGKLTHLIAMRALLYEKLPSKGRVLTLLA
                     N"
     gene            complement(483..752)
                     /locus_tag="AC16FV_08085"
     CDS             complement(483..752)
                     /locus_tag="AC16FV_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850050.1"
                     /translation="MTGYFTPEEIVSQLRDKQPRGKCRHDDKRTILKAQIQQDIARLI
                     CTASDTNYFSYLIAKRGELALVIGVRGDLQNRTVTLELIRPNAGE"
     gene            complement(780..1406)
                     /locus_tag="AC16FV_08090"
     CDS             complement(780..1406)
                     /locus_tag="AC16FV_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR2850051.1"
                     /translation="MSDALNYLVSTRPEAMKAYLKFLKETGKSLDPKTRMLITVITKI
                     ATQTEKGFRQYLPRALDAGATPNEILDAIMHAFPALGLAKIVWAIDILLDMDIPEFRA
                     ENLGAEKRWHDVKPADQIPEGKITYCDSDGRSLFIYRVNGEFNVYDSRCPHQVTNIPH
                     LALEGFRLTCPKHHWAFDIKTGECVEKGNRPLKRFESKVENGRLFARW"
     gene            complement(1555..2838)
                     /locus_tag="AC16FV_08095"
     CDS             complement(1555..2838)
                     /locus_tag="AC16FV_08095"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HIR2850052.1"
                     /translation="MKKKIAIQDLQRGMYVSELVGRHWRETPFLFQGFEVQSDEQIEE
                     LGRYCRHVYIDTEQGHDITSKPRPTASAAPAPIVPAEHKEPVVKFEKVLERFTPGHRR
                     PPRYQDVTVLEEEIGHAREIVTETREAVYDIMGDVRLGRSINTTAAKKVVADMVDSVI
                     RNPDALMCLTQLKDKDEYTALHSLRVCILALAFGRHLDLSDEELNLLGIGALLHDIGK
                     MKVPNDIINKPGKLTDEEFALMRSHVPRGVEILEQTHGISTVSIDVARYHHERYAGGG
                     YALGSQGDQIGLFGSVGAIVDCYDAITSDRSYHTGMSAHDALNKMYSWRGKDFQPLLI
                     EQFIQCMGIYPIGSVVEFNNGSIGVVISINRTRRLKPKVAMVLNSDKTPFTPAKVIDL
                     MQEATEGGIRGLEIRKVLPPGEHGVIPTKYIPLGA"
     gene            complement(2908..3804)
                     /locus_tag="AC16FV_08100"
     CDS             complement(2908..3804)
                     /locus_tag="AC16FV_08100"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HIR2850053.1"
                     /translation="MSRKHPIIAITGSSGAGTTTVKRAFEHIFRRAQINPAIVEGDSF
                     HRYNRAEMREAMKKAEVSGNHHFSHFGPEANLFDELEKQFKTYGETGGGKKRYYLHSD
                     GEAAEHNKRLNTSLKAGEFTPWEDVPRGTDLLFYEGLHGGAVDGKAGVDVAQWVDLLV
                     GVVPIVNLEWIQKIHRDTAERGYSAEAVVDTILRRMYDYVHYITPQFSRTHVNFQRVA
                     VVDTSNPFIARDIPTPDESMVVIRFRDPKKVDFPYLYSMIQGSFMSRPNTIVVPGGKM
                     GFAMEIILTPMIHELLENKKKA"
     gene            complement(3888..5642)
                     /gene="soxB"
                     /locus_tag="AC16FV_08105"
     CDS             complement(3888..5642)
                     /gene="soxB"
                     /locus_tag="AC16FV_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR2850054.1"
                     /translation="MSLSRREFLQMLAMGAAAGLVFEGGLNGRKAFASDKKPGDLYDV
                     APFGNVTLLHMTDSHAQLIPTYYREPNINIGVAGARGKPPHLVGANLLKHFNIKPGSI
                     EAHAFTYLDFEEAARRYGKIGGYAHLATLVKNLRASRPDRTLLLDGGDTWQGSATSLW
                     TRGQDMVDAQKLLGVDIMTAHWEFTYGADRVREIVEKDLKGQIEFLAQNVSDATWGEL
                     IFKPYVIREINKIQVAVIGQAFPYTPIANPRYLVPDWTFGINDDHMQKMVDEVRAKGA
                     QVVAVLSHNGMDVDLKMASRVRGIDVILGGHTHDAVPAPSLVKNAGGKTLVINSGANS
                     KFLAVLDLEVKNGKVTDYRFKMLPIFANLLPPDKDMATYIEKVRAPYADKLGEKLAVT
                     EGLLYRRGNFNGTFDQLIVEALLAVQGADIAFSPGFRWGTTLLPGDTITLEHLMDQTA
                     ITYATATVNHLTGEQIKIIMEDIADNLYHADPYYQQGGDMVRVGGLQYTLDIAQKHGK
                     RVQNPTVGDKPLDPGKKYKVAGWASVQPIEPGKPVWDVVAEYLRARKTVRIDRPYTPR
                     LKGVSDNPGYAVLNTRQT"
     gene            complement(5803..6672)
                     /gene="soxA"
                     /locus_tag="AC16FV_08110"
     CDS             complement(5803..6672)
                     /gene="soxA"
                     /locus_tag="AC16FV_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR2850055.1"
                     /translation="MKKILMLLAALGVVAGTAPATMADPASDLKQFQAFFTKKFPTVK
                     FDEFSNGLYALPGLEEYREQWMLFNEFPPYELGLSLGKKMWDTPFKNGKTFASCFKNG
                     GKNIAQGYPYWDDKTQKVRTAEMDLMDCAHSNGENFSFLNADLSKDQGPRVQLAQLTA
                     YFYSLSQGQRIKIDLSSPGAVKAYEEGKKFWWQRRGQLNFSCANCHMDLAGKNFGGNQ
                     PLSAALGHTTAWPAQRLEWGRLETIHQRYATCNSQVRAMPFKHGAEIYNHLQLYETYM
                     SSGLPLTAPAMRN"
     gene            complement(6727..7047)
                     /gene="soxZ"
                     /locus_tag="AC16FV_08115"
     CDS             complement(6727..7047)
                     /gene="soxZ"
                     /locus_tag="AC16FV_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR2850056.1"
                     /translation="MADIQTKIRTKTQGGVTDVLVLVDHPMETGQRPDPKDKDKKVPA
                     HYIQKLNFSLNGKEVASTDLGPAVAKDPLIGIKLKGAKPGDKIKVTWSDNLGQSGSGE
                     ATVG"
     gene            complement(7087..>7211)
                     /locus_tag="AC16FV_08120"
     CDS             complement(7087..>7211)
                     /locus_tag="AC16FV_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR2850057.1"
                     /translation="YFSARMKMAQTSDVVAVVKAGGKLYSAKQNVKVTVGGCGG"
BASE COUNT         1297 a         2034 c         2216 g         1664 t
ORIGIN      
        1 aggttggctg ctggccgagg tctggcattc tgtggaccgg cccggtgcgt acgcccgagt
       61 cgttgctgca gcttgtgcag acactcaatg ccgcactcac tccatgcggt tacgagccgg
      121 agacacgccc ctatgcggcg cacctgacac tcgcgcgcaa ggtgcgccgg tgccaccacc
      181 gtcatccgat cgaaccgctt gcatgggaag tgcgccagtt ctgccttgtg gagtcgcaca
      241 cgcattccga cggcgcacgt tacgaaattc tccgttcctg gccgcttaat tcgccagcag
      301 cgtgagtacc cgtcccttgg acggcagttt ctcgtagagc aatgcgcgca tggcgatcag
      361 gtgggtcagc ttgccctcgt cggcgagaat cctgagcgcg ttgcgtccgc tgcgctgcgc
      421 catgcattcg cgccggcgca gatcctgaat gctcagaacg ggatcactgt aggaaaaata
      481 agctattcgc cggcgttggg gcggatgagc tcaagggtga ccgtgcggtt ttgcaggtcg
      541 ccgcgcacgc cgatgaccag agccagctcg ccgcgcttgg caatgagata gctgaagtaa
      601 ttggtgtcgc tcgcggtgca gatcaggcgc gcgatgtcct gctgaatctg ggccttaagg
      661 atggtgcgct tgtcatcatg ccggcatttc ccgcgcggtt gtttatcccg cagctgcgag
      721 acgatttcct cgggcgtgaa atatcccgtc atgccgtggc catccgctgg ataaggaact
      781 taccagcgag cgaacaaccg gccattttcc accttggact cgaagcgttt cagggggcgg
      841 ttgccttttt ccacgcactc gccggtcttg atgtcgaagg cccagtggtg cttggggcag
      901 gtgagacgaa agccctcaag cgccaggtgc ggaatgttcg tgacctggtg cgggcagcgg
      961 ctgtcgtaga cgttgaactc gccgttgacg cgatagatga acaggctgcg tccgtcgctg
     1021 tcgcagtagg tgatcttgcc ctcggggatc tggtccgcgg gcttgacgtc gtgccagcgc
     1081 ttttccgcgc ccagattttc ggcgcggaat tccgggatat ccatgtcgag caggatgtcg
     1141 atggcccata cgatcttggc cagccccagc gccgggaaag cgtgcatgat ggcatcgaga
     1201 atttcattcg gcgtggcccc ggcgtcgagt gcgcgtggaa gatactgacg aaagcccttt
     1261 tccgtctggg tggcgatctt ggtgatgaca gtgatcagca tgcgggtttt gggatcgagc
     1321 gacttgcccg tctccttgag aaacttcagg taagccttca tcgcctcggg gcgggtgctg
     1381 accagataat ttaatgcgtc actcatccag aactctcctg ttatttacct tataaagcga
     1441 aaggacaatg tcctgcgcgg ccagatatta tgcgcagcac acatgcgatg tgccagtcgt
     1501 ccaggtgcac gagacaacgg tcttttacgc gtatgacgta tgcgcgcccg tgtctcaggc
     1561 gccgagcgga atgtatttgg tgggaatgac gccgtgctcg cccggtggga ggaccttgcg
     1621 gatctccaga ccccggatgc cgccttcggt ggcctcttgc atcaggtcaa ttaccttggc
     1681 tggggtgaag ggcgtcttgt cggaattgag gaccatcgcc actttgggtt tcaggcggcg
     1741 tgtgcggttg atcgagatga ccaccccgat gctgccgttg ttgaattcga ctacgctgcc
     1801 gatgggatag atgcccatgc attgaatgaa ctgttcgatc agtagcggct gaaagtcctt
     1861 gccgcgccat gagtacattt tgttgagcgc gtcgtgcgcc gacatgccgg tatggtagga
     1921 acggtccgag gtgatggcgt cgtagcagtc cacgatggcc ccgacggagc cgaagagccc
     1981 gatctgatcg ccttgggaac cgagggcata accgccgccc gcgtaacgtt cgtgatggta
     2041 acgcgcgacg tcgatggaaa cagtagagat gccgtgggtc tgttccagga tttccacgcc
     2101 ccgcgggacg tggcttctca taagggcaaa ctcctcgtcg gtaagcttcc cgggtttgtt
     2161 gatgatgtca ttcggcactt tcatcttgcc gatgtcgtgc agcagcgcgc cgatgccaag
     2221 cagattcagc tcttcatctg aaagatcgag gtgtcgaccg aaggcgagcg ccaggatgca
     2281 gacccgcaga ctgtgcagtg cggtgtactc gtccttgtcc ttgagctggg tgaggcacat
     2341 gagcgcgtcc gggttgcgaa tgacgctgtc caccatgtcg gccacgacct tcttggcggc
     2401 cgtggtgttg atgctgcggc ctagacgcac atcgcccatg atgtcgtaaa cggcctcgcg
     2461 ggtttcggtc acgatctcgc gagcgtggcc gatctcctct tcgagcacgg tgacatcctg
     2521 gtaccgtggc gggcgccgat gacctggcgt gaaacgttcc agcacctttt cgaatttgac
     2581 gacgggctct ttatgctccg caggcacaat cggggccggc gcggcgctgg cggttgggcg
     2641 gggcttcgac gtgatgtcgt gcccttgttc agtgtctatg tagacgtgcc ggcaataacg
     2701 accgagttcc tcgatctgct cgtccgactg gacctcgaag ccctggaaca ggaacggcgt
     2761 ttcacgccaa tggcgtccca ccagctcgga gacatacatg ccgcgctgaa gatcctgaat
     2821 ggcaatcttc tttttcatat cataaggata gcagcaagat tcgcgccaat tcggcgcgaa
     2881 ttttactacc cggacggtgg cgacggttta agctttcttt ttgttttcaa gcagttcatg
     2941 aatcatcggc gtgaggatga tttccatggc gaagcccatc tttccgccag gcacgacgat
     3001 ggtattggga cgagacatga acgacccctg gatcatgcta taaaggtagg ggaaatcgac
     3061 cttcttgggg tcgcggaacc ggatgacgac catgctctca tcgggggtcg ggatgtcgcg
     3121 cgcgataaag gggttggagg tgtccaccac cgccacacgc tggaaattga catgcgtgcg
     3181 cgagaactgg ggcgtgatgt agtgcacata gtcgtacatg cggcgcaaaa tggtgtcgac
     3241 caccgcctcg gctgaatagc cgcgctcggc ggtgtcgcgg tgaattttct gtatccactc
     3301 gagattgacg atcggcacca cgcccacgag cagatccacc cattgggcca cgtccacgcc
     3361 ggcttttcca tccacggcgc cgccgtgcag tccttcataa aacagaagat cggtgccgcg
     3421 cgggacatct tcccagggag tgaactcgcc ggccttgagg ctggtgttga ggcgcttgtt
     3481 gtgctccgcc gcttcgccgt cgctgtgcag gtagtagcgc ttcttgcctc cgccggtttc
     3541 gccgtaggtc ttgaactgtt tttccagttc atcgaacaga ttggcctcgg gtccgaagtg
     3601 actgaaatga tgattaccgc tgacctccgc ctttttcatc gcctcgcgca tctcggcgcg
     3661 attgtagcgg tgaaagctgt caccctccac gatggccgga ttgatttgcg cccgccggaa
     3721 aatgtgctcg aaggcgcgct tgacggtggt ggtgccggca ccgcttgaac cggtgatggc
     3781 gatgatcggg tgcttcctgg acatcgttga taaccccttg ggtcgctaaa agcggcacat
     3841 tctacatcag ggcgattagc ggataccacc cgcggggcgg aggtgtctca ggtttggcgg
     3901 gtatttaata cggcataccc cgggttgtcg gatacacctt tcaggcgcgg ggtataggga
     3961 cggtcgatgc ggacggtttt tcgggcgcgc agatattcgg cgacgacgtc ccatacgggt
     4021 ttaccgggct cgatcggctg cacgctggcc caaccggcga ccttgtattt cttgccgggg
     4081 tcgagcggct tgtccccgac cgttggattc tggacgcgct tgccgtgttt ctgggcgatg
     4141 tcgagcgtgt attgcagtcc accgacacgc accatgtcgc cgccctgctg ataataagga
     4201 tcggcgtggt acaaattgtc ggcgatatct tccatgatga tcttgatctg ttcaccggtg
     4261 agatggttga cggtggcggt ggcgtaagtg atcgcggtct gatccatcag gtgttccagg
     4321 gtgatcgtgt cgcccgggag cagcgtggtg ccccagcgga agccgggcga gaaggcgatg
     4381 tccgcgcctt gcaccgccag cagcgcctct acgatcagct gatcgaaggt gccgttgaaa
     4441 ttgccgcgcc ggtagagcag gccttccgtt accgcgagtt tttccccgag cttgtctgcg
     4501 taaggcgcgc gcaccttctc aatgtaggta gccatgtcct tgtcgggcgg gagcaggttg
     4561 gcgaatatcg gcaacatttt gaagcggtag tcagtcacct tgccgttctt gacctcgaga
     4621 tcaagcaccg ccaggaattt gctgttggcg ccggaattga tgaccagcgt tttgccgccg
     4681 gcgtttttca ccagcgacgg tgccggcacg gcgtcatgtg tatgcccgcc gaggatgacg
     4741 tcgataccgc gcacacggct ggccattttg agatccacgt ccatgccgtt atgcgacagc
     4801 accgcgacca cctgcgctcc cttggcgcgc acctcgtcga ccatcttctg catgtggtcg
     4861 tcgttgatgc cgaaggtcca gtccggaacg agatagcgcg gattggcgat cggcgtgtac
     4921 ggaaatgcct gtccgatgac ggcgacctgg attttgttga tctcacgaat gacataaggc
     4981 ttgaaaatca gctcgcccca cgtggcgtcg ctcacgtttt gcgccaggaa ttcaatctgg
     5041 cctttcagat ccttttcgac gatttccctg acgcgatcgg cgccataggt gaattcccag
     5101 tgcgcggtca tgatgtctac gccgagcagt ttctgcgcgt ccaccatgtc ctgtcccctg
     5161 gtccagagtg aggtcgccga gccctgccag gtatcgccgc cgtccagcag cagcgtacgg
     5221 tcaggacggc tggcgcgcag atttttcacc agcgtggcca gatgcgcgta gccgccgatc
     5281 ttgccgtaac ggcgcgcggc ttcctcgaaa tcaaggtagg tgaaggcatg cgcctcgatc
     5341 gatccgggct tgatgttgaa atgtttgagc aggttcgcac cgaccagatg cggcggtttc
     5401 ccgcgggcgc cggcgacacc gatattgatg ttgggttccc tgtagtaggt cggtatgagt
     5461 tgcgcgtggg agtccgtcat gtgcagcagc gtgacatttc cgaatggagc cacgtcataa
     5521 agatcacccg gttttttgtc gctggcgaat gccttgcggc cattcaaacc gccctcgaaa
     5581 acgaggccgg cggcggcccc catggcgagc atttgcagaa attcgcgtcg ggataggctc
     5641 ataggatcat gcttttattc ggtgtggttt tacgtcgggg cagcgtcatc atgaactgcg
     5701 ttctacttaa gacgtccggt tacaaactcg tattcctcaa ctgccggaaa agaaaacccg
     5761 gcgttacatc gcgccgggtt ttggcgaaca atcctgccgc ctctagttgc gcatggccgg
     5821 cgccgtcagg ggcaggccgc tgctcatgta ggtctcatag agctggagat ggttgtatat
     5881 ctcggctccg tgcttgaagg gcatggcgcg cacctgcgag ttgcaggtgg cataacgctg
     5941 atggatggtc tcgaggcgac cccattccag gcgctgggcc ggccaggccg tggtgtggcc
     6001 gagcgcggcg ctgagcggct ggttgccgcc gaagtttttg cccgccagat ccatgtggca
     6061 attggcgcat gagaaattga gttgaccacg gcgttgccac cagaatttct tgccttcttc
     6121 gtaggctttc acagcgccgg gactggaaag atcaatcttg atgcgttgtc cctgcgagag
     6181 cgagtagaaa taggccgtca gctgggccag ctgcacgcgc gggccctggt ccttgctcag
     6241 gtcggcgttg aggaaggaaa agttttcgcc gttactgtgc gcgcagtcca tcaggtccat
     6301 ttccgcggta cgaacctttt gcgttttatc gtcccagtag gggtagcctt gggcgatgtt
     6361 tttgccccca ttcttgaagc agctggcgaa ggtcttgccg ttcttgaacg gcgtatccca
     6421 catcttcttg cccagggata gcccgagctc atagggcggg aattcattga acagcatcca
     6481 ttgctcgcgg tattcctcga ggcccggcag ggcatagagg ccgttcgaga attcatcgaa
     6541 cttgaccgtg gggaatttct ttgtgaaaaa ggcctggaat tgcttgagat cggaggccgg
     6601 atcggccatg gtcgccggcg ccgtcccggc aacaacaccc agagcagcca gaagcattaa
     6661 aatttttttc atcgcgggtt ctccttttgg tgaattcacg gaccggataa agcgacgctc
     6721 ctcgtttcag ccgacagtgg cttccccgct gccgctttgc cccaggttgt cactccaggt
     6781 gaccttgatc ttgtcgcccg gtttggcgcc cttgagtttg atgccgatca acggatcttt
     6841 cgctaccgcc gggccaagat cggtggaggc aacttccttg ccgttcaggg agaaattcag
     6901 tttctggata tagtgtgccg gaaccttctt gtccttgtcc ttggggtctg gacgctgtcc
     6961 ggtctccatc ggatgatcaa ccagcaccag gacgtccgtt acgccgcctt gtgttttggt
     7021 ccggattttc gtctgtatgt ctgccatgtg ttttttcctc agcgatattc gtttgccgtt
     7081 acggactcaa cccccgcacc cgcccaccgt caccttcacg ttctgcttgg cggagtagag
     7141 cttgcccccg gccttgacca cggcgaccac gtccgaggtc tgggccatct tcatgcgggc
     7201 cgagaagtag c
//
LOCUS       DBNGZE010000312         5669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1363712, whole genome shotgun sequence.
ACCESSION   DBNGZE010000312 DBNGZE010000000
VERSION     DBNGZE010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1363712"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..358
                     /locus_tag="AC16FV_08125"
     CDS             62..358
                     /locus_tag="AC16FV_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR2850058.1"
                     /translation="MHVTVVHVQVKPEHIQDFIDASRLNHEASIREPGNRRFDVAQSS
                     DDPSRFILYEAYASAEAAAAHKSTPHYLKWRDAVAPWMDAPRVGTPYIGIFPGD"
     gene            358..1557
                     /locus_tag="AC16FV_08130"
     CDS             358..1557
                     /locus_tag="AC16FV_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR2850059.1"
                     /translation="MFPIGAFSVSRLPRIVFGAGAIRQLPAIVAEYGDRLLIVTGARS
                     LRESASWSSLLKQLAAAIISVDDMKVEGEPSPRLVDDAVSAYRRKNINVVLGIGGGSA
                     LDAAKAIAGLLPSGRSVMDHLEGVGRDIPYTGPATPFIAVPTTAGTGSEATKNAVLSV
                     QGTDGFKKSFRHDALVAQVAVVDPELLATCQPEVIAANGMDAFTQLLESYVSARANPF
                     TDALAFSGMQAVRDGFFDAWRGGDSREAAQGRACMAYAALLSGVTLAQAGLGSVHGLA
                     SPLGAYFPIPHGVVCGTLVATATEVNIRALRNRAPENPALEKYAEVGKLLAGKSPSCR
                     EEAWDELVRILHEWTARLKIPALGTYSVNNSDVEKIVAGSRGSSMKTNPVVLTDEEIA
                     EVVRKRL"
     gene            complement(1564..4011)
                     /locus_tag="AC16FV_08135"
     CDS             complement(1564..4011)
                     /locus_tag="AC16FV_08135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2850060.1"
                     /translation="MEGLGLNSFRGRLLYVAALSICFMLIATWLAEKQVSEATRRGIS
                     DAADRQEINRLLHDVSNDIWAAETSLHHAPESGLSRGAITLTHLRSALAKSAPLADIM
                     KRQRAPAAQQETQQFIASLHQLHKEVTAGETSGLPLTTLHARAEPFLERVRTTLFMLE
                     KTLAAHTMEDMQTLAQAVEHQLRSLWIATLVGIVGIMAGFFLFEYCIRKPVVRITGAL
                     KAEAEGSKQIKISLTSTQETANLVSAFDHMRRQVHARQQRLETILDNAAEGIITFDAD
                     GRIESFNKASERLFGYTEEEVLERDLRLILPPQTRESREDYLKHFIRTEIQHLIGHEG
                     EVTGRHKDGTTFPMALKISAMMLEGKQLYTGLVADISERKAMLDNLKNMAEHDGLTGL
                     YNRSYFQQELERVVERVKRAGGMCCSLLYIDLDNFKYVNDTLGHAAGDRLIIEVASIL
                     NKRARKSDIIARFGGDEFTVLLFDTTPMLSTQVAESFRARLADYIFKHSGEQIDIGCS
                     IGVTGISPGTTSATEVLSQADLACHLAKRGGRNRVHLYNPEDAENVAAMSLDMGWSRR
                     IKEAIEKDRFVLACQPIVSTVTREVESYEVLIRMLDERNELIMPSGFLATADRFGLAA
                     DIDKWVIRHAIEGLARQRVKSPSLRYAINLSGRTLTTPGICELIRDGLKDNGLDPSAL
                     TFEVTETVAIADMAAAEIFLARLRSMGCHTALDDFGSGMASFAYLKDLPVNCVKIDGR
                     FVKNLATSPVDQAMVKAMNEIAHALGKQTIAEFVENEESFKLLAAYGVDFAQGYHLGR
                     PDVTLPCSALTQGRCAL"
     gene            4296..>5669
                     /gene="ppsA"
                     /locus_tag="AC16FV_08140"
     CDS             4296..>5669
                     /gene="ppsA"
                     /locus_tag="AC16FV_08140"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR2850061.1"
                     /translation="MRYIRTFSELGINDVPVVGGKNASLGEMFRHLSAEGVRVPDGFA
                     TTADAYRHYLEHNRLTERIQARLKGVASDDLAKLEQAGADIRGWILDAGMPPDLAGEI
                     TTAYENLARQYGAEPDVAVRSSATAEDLPNASFAGQQDTFLNIRGAENLLFVCRRVFA
                     SLFNDRAISYRVHQGFDHAQVALSIGVQKMVRSDMASSGVMFTLDTETGFRDVVFINA
                     AYGLGENVVQGAVNPDEFYVFKPTLMAGKRPILKRHLGSKAMKMTYAQDVADGNSTRN
                     IPVPEVERQRFSVTDDEALELARYAVKIEQHYSKLAGQDRPMDIEWAKDGTDGKLYIL
                     QARPETVRSREARDVYEVYALESRGKVLATGKSVGRRIAGGAAHVIETAADMSKLRAG
                     EVLVTDMTDPDWEPVMKTAAAIVTNRGGRTCHAAIVSRELGIPCVVGTEVATARLVDG
                     QQVTVSCA"
BASE COUNT         1068 a         1645 c         1785 g         1171 t
ORIGIN      
        1 gcgcaagagg tatcggtagt agatttcccg caagaaaaaa taccacaccg caggaggagc
       61 catgcatgtg accgttgtcc atgtgcaggt caaaccggag cacatacagg actttatcga
      121 cgccagccgg ctgaaccacg aggcgtccat ccgcgagccg ggaaatcggc gtttcgacgt
      181 ggcgcagtct tccgatgatc cttcccggtt cattctttac gaggcctacg ccagcgccga
      241 ggccgcggcc gcgcacaaaa gcacgccgca ttatctgaaa tggcgcgatg ctgtcgcgcc
      301 gtggatggac gcgccgcgtg tcggcactcc ttatataggg attttccccg gagactgatg
      361 ttccccatcg gcgccttctc cgtttcacgc ttgccgcgca ttgttttcgg cgccggtgcc
      421 atccggcaac tgcccgccat cgtcgcggaa tacggcgatc gtctgctgat cgtgaccggc
      481 gcgcgttcgc tgcgcgagtc ggcgagctgg tcgtccctgt tgaagcaact ggcggccgcg
      541 attatctctg tggatgacat gaaggtagag ggtgagccct cgccacgact cgtagatgac
      601 gccgtttccg cctatcgccg gaagaacatc aatgtagttc tcggcattgg tggcggcagt
      661 gcgctggatg ccgccaaggc gatcgcgggt cttttgccca gcggccgttc ggtcatggat
      721 catctggaag gtgttggccg cgatattcct tacaccggac cggccacacc gttcattgcc
      781 gtaccgacca ccgctggcac gggcagcgag gccaccaaga acgccgtgct cagcgtgcag
      841 ggtacagacg gtttcaagaa atcctttcgc catgacgcgc tggtggcgca ggtggcggtg
      901 gtagatccgg aactgctggc cacctgccag ccggaagtga tcgccgccaa tggcatggat
      961 gccttcacgc aattgctgga atcctacgtt tcagccaggg ctaatccatt taccgacgcg
     1021 ctcgccttct ccggcatgca ggcggtgcgg gatggattct tcgatgcctg gcgtggcggc
     1081 gacagtcggg aagcggcgca gggaagggcc tgcatggcct acgccgcgtt gctttccggc
     1141 gtcacgctgg cacaggcagg gctcgggtcg gtgcacggat tggcttcacc gctgggcgct
     1201 tattttccga ttccgcacgg cgtggtttgc ggcacgctgg tggcgaccgc gacggaggtg
     1261 aatatccgcg cgctgcgaaa ccgggctccg gaaaatccgg cgctggaaaa atatgccgag
     1321 gtcggaaaat tattggctgg aaaatcgcca tcatgccggg aagaggcatg ggatgaactg
     1381 gtgcggatac tccatgaatg gaccgcgcgg ctcaaaattc ctgcgctggg aacttattca
     1441 gtgaacaatt ccgatgtgga gaaaattgtc gccggttcgc gtggcagcag catgaagacc
     1501 aatccggttg tgctgacgga cgaggagatt gccgaagtgg tcaggaagcg cttgtgaccg
     1561 cgactacagg gcgcaacgtc cctgcgtgag cgcggaacag ggcagcgtca cgtccggtct
     1621 tcccaggtgg taaccctgcg caaagtccac gccataggcg gcgagaagtt tgaagctctc
     1681 ctcgttttcc acgaactcgg cgatggtctg cttgcccagc gcgtgggcga tttcgttcat
     1741 ggccttcacc atcgcctgat ccaccgggct ggtcgcaagg ttcttcacga aacggccgtc
     1801 aatcttgacg cagttcacgg gcaggtcctt gaggtaggca aatgaggcca tgcccgaacc
     1861 gaagtcgtcc agcgcggtat ggcaacccat ggagcgcaag cgcgcgagaa agatttcggc
     1921 cgccgccatg tcggcgatgg ccacggtttc cgtcacctcg aatgtgagag cggagggatc
     1981 caggccgttg tccttgagcc catcccggat cagctcgcag atacccggcg tggtcagggt
     2041 gcgtcccgac agattgatgg catagcgcag ggagggcgat ttgacgcgtt gccgcgccaa
     2101 gccctcgatg gcgtgacgga taacccattt gtcgatgtcc gccgccagcc cgaagcgatc
     2161 ggcggtggcg aggaaacccg agggcatgat gagttcgtta cgttcgtcga gcatgcggat
     2221 cagcacctcg taggactcga cttcgcgcgt gacagtgctg acgatcggct ggcaggcaag
     2281 cacgaaccgg tccttttcaa tcgcctcctt gatgcgccgt gaccagccca tgtcgagcga
     2341 catggcggcg acgttctcgg cgtcctcggg attgtacaga tgcacgcgat tccggccgcc
     2401 gcgtttggcc agatggcagg caaggtccgc ctgcgaaagc acctcggtgg ccgaagtcgt
     2461 gcccggactg atgccggtaa cgccaatgga acagccgatg tcgatttgtt cgccgctgtg
     2521 cttgaagatg taatcggcca gccgcgcgcg gaaggattca gccacctggg tggacagcat
     2581 gggcgtggtg tcgaacaaca gcaccgtgaa ttcatcgccg ccaaagcggg cgatgatgtc
     2641 gcttttgcgc gcgcgcttgt tgagaatgct ggcgacctcg ataatcagcc ggtcgccagc
     2701 cgcgtgcccg agcgtgtcgt tgacgtattt gaagttgtcg aggtcgatat aaagcagcga
     2761 gcagcacatg ccgcccgcgc gcttgacacg ctccacgacg cgctccagtt cctgctggaa
     2821 gtaactgcgg ttataaagcc ccgtgagtcc gtcatgctcg gccatgttct tgagattatc
     2881 cagcatggcc ttgcgttcgc tgatgtccgc caccagtccg gtgtacagct gtttcccctc
     2941 cagcatcatg gcgctgatct tgagcgccat cgggaaggtg gtcccgtctt tatggcggcc
     3001 ggtgacctcg ccttcatgcc cgatcaggtg ctggatttca gtgcggatga aatgtttcag
     3061 ataatcctcg cgactctccc gtgtctgtgg cgggagaatg agtcgcagat ccctttcgag
     3121 cacttcctcc tccgtgtagc cgaacaatct ttccgatgcc ttgttaaagc tttcgatgcg
     3181 gccgtcggcg tcaaaggtga tgatgccctc ggcggcgttg tccagaatgg tttccaggcg
     3241 ttgctggcgt gcgtgcacct gccggcgcat atggtcgaag gcggatacca gattcgccgt
     3301 ctcctgcgtc gaagtgaggg atatcttgat ttgtttcgaa ccctccgctt cggccttgag
     3361 ggcgccggtg atgcgcacga ccggtttgcg gatgcaatac tcgaacagga aaaaccccgc
     3421 cataatcccg acgataccca ccagcgtggc aatccagagc gagcgcaact gatgctcgac
     3481 cgcttgcgcc agcgtttgca tgtcttccat ggtatgggct gccagcgtct tctccagcat
     3541 aaacagcgtg gtccggacac gctccagaaa tggttcggca cgggcatgta atgtggtcaa
     3601 cggcagaccg gaggtctccc ccgcggtgac ctctttatgc aactggtgaa gactggcgat
     3661 gaattgctga gtttcctgtt gcgcggcagg agcgcgttgt cttttcatga tgtccgccag
     3721 cggagcggac ttcgccagcg cgcttctcaa atgcgtcagg gtgatggcgc cacgcgacag
     3781 gcccgattcg ggcgcatggt gcagactggt ttccgcggcc cagatgtcgt ttgacacgtc
     3841 atgcaataac cggttgatct cctgccgatc ggcggcatcg gaaattcccc gccgcgtggc
     3901 ttcgctgacc tgcttttccg cgagccaggt agcgatcagc atgaaacaga tggacaacgc
     3961 cgcgacatac agcaggcggc cgcgaaagct gttcagcccc agaccttcca cgatccgcgc
     4021 cgtcagtggg tgtttatgtt gcgatgtttc agatggatta gcggcggcct gcattcaaaa
     4081 aggacgtaca cccggttttt tgttcaagat gcaagcatag atcataccgg ccggcatggt
     4141 ggggattggc gcccgtggac gactatgttc tatttgtata agccgctgtt tctcttgttt
     4201 tattgttgat taatcccgtc gacattctcc gggcgatcct gttcaaaggg ttttattgga
     4261 aatccccccg gcatgggtta ttttgtgccc ccggcatgcg ttatatccgc acattttccg
     4321 agttggggat aaacgacgtc cccgtggtcg gaggtaagaa cgcttccctg ggcgagatgt
     4381 ttcgccactt gagcgccgag ggtgtgcggg ttcccgacgg attcgccacc accgccgatg
     4441 cctatcggca ttatctcgaa cacaatcgcc tcaccgaacg tatccaggcg cgcctcaagg
     4501 gcgtcgcctc ggacgatctc gcaaagctgg aacaggccgg cgccgacatt cgcggctgga
     4561 ttctcgatgc cggcatgccg ccggacctgg ccggggaaat cacgacggcg tacgagaacc
     4621 tggcgcgaca atacggggcg gagccggacg tggcggtacg cagttcggcc acggccgagg
     4681 acctgccgaa cgcctctttc gccggccagc aggacacttt tctaaacatc cgcggcgcgg
     4741 aaaatctgtt gttcgtctgt cgccgcgttt tcgcctcgct cttcaatgac cgcgcgattt
     4801 cctatcgcgt gcatcagggc ttcgaccatg cgcaggtggc gctctccatc ggcgtgcaga
     4861 agatggtgcg ttccgacatg gcctcctccg gtgtgatgtt cacgctcgat accgaaaccg
     4921 gattccgcga cgtcgttttc atcaatgctg cctacggtct cggcgaaaat gtggtgcagg
     4981 gcgcggtcaa tccggacgag ttttatgttt tcaagccgac attgatggcc ggcaagcgtc
     5041 cgatcctcaa gcgccacctc ggtagcaagg cgatgaagat gacctatgcg caggatgtcg
     5101 cggatggcaa ttcgacacgg aacatcccgg tgccggaagt ggagcgtcag cggttctccg
     5161 tgacggatga cgaggcgctg gagctggcgc ggtacgccgt gaagatcgag caacactatt
     5221 cgaaactcgc gggccaggat agaccgatgg atatcgaatg ggccaaggac ggcacggatg
     5281 ggaagctcta tattctgcag gcgcgtcccg agacggtgcg ttcgcgtgag gcgcgcgatg
     5341 tgtacgaggt ttatgcgctg gaatcgcgtg gcaaggtatt ggctaccgga aaaagcgttg
     5401 gccgtcgcat tgcgggcggg gcggcgcacg tgatcgagac agcggctgac atgtcgaaat
     5461 tgcgtgccgg tgaagtgctg gtgacggaca tgaccgatcc tgactgggag ccggtgatga
     5521 aaaccgccgc cgccatcgtc accaaccgcg gcgggcgcac ctgccacgcc gcgatcgtga
     5581 gccgggagct cggcatcccc tgcgtcgtcg ggacggaggt cgcgacggcg cggctcgtgg
     5641 acgggcagca agtcacggtg tcgtgcgcc
//
LOCUS       DBNGZE010000313         3542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_600583, whole genome shotgun sequence.
ACCESSION   DBNGZE010000313 DBNGZE010000000
VERSION     DBNGZE010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_600583"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /gene="coaBC"
                     /locus_tag="AC16FV_08145"
     CDS             complement(<1..576)
                     /gene="coaBC"
                     /locus_tag="AC16FV_08145"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR2850062.1"
                     /translation="MGTLTNKRILLGVTGGIAAYKSADAVRRLREAGAEVRVIMTAGA
                     TEFITALTLQAVSSHPVYQHLLDTDAEAGMGHIELARWADAVLVAPTSANFLARLAQG
                     RADDLLSAVCLATESPVAVAPAMNQQMWSNNATQTNLATLKKNGVRIFGPAEGSQACG
                     EVGPGRMLEPADLVVMISELFASGELDGLTEV"
     gene            700..1404
                     /gene="radC"
                     /locus_tag="AC16FV_08150"
     CDS             700..1404
                     /gene="radC"
                     /locus_tag="AC16FV_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR2850063.1"
                     /translation="MGNKNTKNSPKAISQWPEHERPREKLLHRGPAALSDAELLAIFI
                     RTGVPGKTAVDVARDLIERYGGLRELLAAGPEELCETPGLGEAKYVQLQASLEMGRRF
                     LAEKLKREITLGSTRETREFLQAQLRDRKNEVFCVLFLDNRHRVLVFEELFQGTLNGT
                     AVYPREIVKRALKHNAAAVILVHNHPSGVAEPSRADELLTNRLKEALTLVDIRLLDHL
                     IVGDGETVSFSERGLI"
     gene            1589..1825
                     /gene="rpmB"
                     /locus_tag="AC16FV_08155"
     CDS             1589..1825
                     /gene="rpmB"
                     /locus_tag="AC16FV_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR2850064.1"
                     /translation="MSRVCQVTGKRVRVGNNVSHANNKTRRRFLPNLQNRRLWVESEK
                     RWITLRLSSNGLRTIDKKGIDAVLAEMRARGEKV"
     gene            1963..2130
                     /gene="rpmG"
                     /locus_tag="AC16FV_08160"
     CDS             1963..2130
                     /gene="rpmG"
                     /locus_tag="AC16FV_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR2850065.1"
                     /translation="MAKSAREKIKLVSSAGTGHYYTTTKNKRTTPDKLEFKKYDPVVR
                     KHVAYKEAKIK"
     gene            2361..2915
                     /locus_tag="AC16FV_08165"
     CDS             2361..2915
                     /locus_tag="AC16FV_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2850066.1"
                     /translation="MPLEWIDFKEAVRVYHLGQVAYTCGVPLYKIRGGHNAITGKRSI
                     LEINSIVATHGDSHAVLKARAHYVPPLNNQTLFARDAHLCLYCGEQFSSRGLSRDHVI
                     PLSVGGTDTWNNVVTACKRCNNHKAGRTPEQAGLELIAVPFMPTHAEYIYLQGKRILA
                     DQMQFLLAHFPRHSPLHKRLARVA"
     gene            complement(3063..>3542)
                     /locus_tag="AC16FV_08170"
     CDS             complement(3063..>3542)
                     /locus_tag="AC16FV_08170"
                     /inference="COORDINATES: protein motif:HMM:NF049919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA/ISL3 alpha bundle tail domain-containing
                     protein"
                     /protein_id="HIR2850067.1"
                     /translation="GLARVKKIPPELTCDTAKQHIDLDTVRAVITGRFVVMAQFAREV
                     MQHVHREELKKVDRADRESWALIKRARRLMVREAAQLDETKHQLLQRALELNDRLKTV
                     YTMKQKLVDIWNRRATTQEHLIQALQEWCREAEATGIEALRHFSQKLRTYTLAPVPA"
BASE COUNT          722 a         1052 c         1075 g          693 t
ORIGIN      
        1 aacctcggta agtccatcga gttcgccgct ggcgaacaat tcggaaatca ttaccaccag
       61 atcggccggt tccagcatgc ggccgggccc gacttcgccg caggcctgcg agccttccgc
      121 cggcccgaaa attctgacgc catttttctt gagtgtcgcc aggttggtct gggtcgcgtt
      181 gttgctccac atctgctgat tcatggcagg cgcgacggcc acgggcgatt cggtcgcgag
      241 acaaaccgcg cttagcagat cgtcggcgcg gccttgcgcc agacgcgcca gaaaattcgc
      301 gctggtgggc gccaccagca cggcgtcggc ccagcgcgcg agttcgatgt gacccatgcc
      361 cgcctcggca tcggtgtcga ggaggtgctg atagactggg tggctgctca ccgcctgaag
      421 ggttaaagcc gtgatgaact ccgtcgcccc ggccgtcatg atgacgcgca cctcggcacc
      481 ggcttcgcgc aggcgccgta cggcgtctgc gcttttatac gcggcaatac cgccagtgac
      541 gccgagcagg atgcgtttat tagtaagtgt acccatatgt taaagtgtac ttgcctggga
      601 gcgagtgaac aaatttgccg ggagcaaatt tggaccgcca tacgcgggcc cgagccgtat
      661 ccccggcgag ggcgagcccc agggaggggg cgagcacaaa tgggtaataa aaataccaag
      721 aatagcccga aggccatcag ccaatggccg gagcatgagc gcccgcgcga gaagcttctg
      781 catcgcggcc cggcggcgct gtcggacgcc gagttgctgg ccattttcat ccgcaccggc
      841 gtgcccggta aaaccgccgt ggacgtggcg cgcgatctga tcgagcgtta tggcggcttg
      901 cgcgaattgc tggccgccgg tccagaggaa ttgtgcgaga cgccggggct cggcgaggcc
      961 aaatacgtgc agctgcaggc aagcctggaa atgggccggc gctttctggc ggaaaaactc
     1021 aagcgcgaaa tcacgctggg ttcgacccgg gagactcggg aattccttca ggcgcaactg
     1081 cgggaccgca agaacgaggt gttctgtgtc ctgtttctgg acaaccgcca ccgggtgctg
     1141 gtgttcgagg agctgttcca gggcaccctg aacggcacgg cggtttatcc gcgcgagatc
     1201 gtcaagcgcg cgctcaaaca caacgccgcc gccgtgatct tggtgcacaa ccacccctcg
     1261 ggggtggccg agcccagccg ggcggacgaa ctcctgacca accggctcaa ggaagcgctg
     1321 actttggtgg atatccggct gttggatcat ttgatagtag gggatggcga aaccgtctct
     1381 ttcagtgaga gaggcctgat ataggcattt ctgttaaatc agaggggtcc ctgggcccta
     1441 ccatgcaccc gtcttttttg acgtggacca tccctggtcc acgccctgcg ggcgcgctaa
     1501 agcgcgtcca attttggttc ccgacaaaat tgtgatataa cagccggcct ttccggggtt
     1561 ccggacgatt gttttaggga gattttcgat gtcgagagta tgtcaggtga cgggcaaacg
     1621 agtgcgcgtg ggcaataacg tgtcccatgc gaacaacaag acccgccgcc gttttctgcc
     1681 gaatctgcaa aatcgccgcc tgtgggtgga gagcgagaag cgctggatca cgctgcgcct
     1741 gtccagcaat gggctgcgca ccatcgacaa gaaaggcata gatgccgtgc tggccgagat
     1801 gcgcgcccgc ggcgagaagg tatagggcga gccgaaaacg gacgaagaca cttaacgtgt
     1861 cttcgttccg gcgagcgccc gtacatggag gtacgggccg gggtaaatcg aaacctatcg
     1921 gcaacgccat ggatggcatg acaagattta ttggaggttt atatggccaa atcagcacgt
     1981 gaaaagatca agctggtctc cagcgccggg accggtcatt actacaccac gaccaagaac
     2041 aagcgcacca cccccgacaa gctcgaattc aagaagtacg acccggtggt ccgcaagcac
     2101 gtggcctaca aggaagccaa gatcaagtag gcatgacccc ttgtgattct gaaaacaaag
     2161 cccggctctg ccgggctttg tttttttgcg ctatccttaa aaaaggaagt aacggggcag
     2221 gccgacctgg gcgtaatggt ccggctaggt acaaccccct ggttttctga gataactacg
     2281 gcccgtatcc gctggataag gtagggaacg tcgctgtgta tgcactgaat cagcaagttt
     2341 tgaggaccga cgcatccggg atgccgcttg aatggattga cttcaaagag gccgtgcggg
     2401 tctatcactt ggggcaggtg gcctacacct gcggggtgcc cctgtataaa atccgcggtg
     2461 gtcataacgc catcaccggc aaacgctcca ttttagaaat caattccatt gtcgccacgc
     2521 acggcgacag ccacgcggtc ctgaaggccc gggcgcacta cgtcccgccg ctcaacaatc
     2581 aaacgctgtt cgcccgcgac gcgcacctgt gcctgtactg cggtgagcag ttttcctccc
     2641 gcggcctgtc gcgtgaccac gtcatcccgc tgagtgtggg cggcaccgac acctggaaca
     2701 acgtcgtcac cgcctgcaag cgctgcaaca accacaaggc cggccgcact ccggagcagg
     2761 cgggactgga gctgattgcg gtgccgttca tgccgacgca cgccgagtac atctatctcc
     2821 agggcaagcg gatcctggcc gaccagatgc aattcctgct ggcgcacttc ccgcgtcata
     2881 gcccgctgca caagcgcctc gccagagtgg cctgaggaaa ttattcccca acaaaaaagc
     2941 ccgcatgatg cgggcttttt tgttcgcccc atccttgggg ctcaccctcg caaaatacgc
     3001 tcgggccggc ctacggccgt ccaattttgt tcatgacaaa attgtgttgg ttcaagggat
     3061 tgtcaggctg ggaccggcgc cagcgtataa gtgcgcagtt tctgcgagaa atgacgcagc
     3121 gcttcgatgc cggtggcttc ggcctcgcgg caccattcct gcagcgcctg gatgagatgc
     3181 tcctgggtcg tggcgcggcg attccagata tccacgagct tctgtttcat ggtgtatacg
     3241 gttttaagcc ggtcattcag ctccagcgcc cgctgcagca actggtgctt ggtctcatcg
     3301 agttgcgctg cttcgcgcac catcagtcgc cgtgcacgct tgattaaagc ccaggattcg
     3361 cggtcggcgc ggtcgacctt cttcagctcc tcgcgatgca catgctgcat gacttcgcgc
     3421 gcgaactgcg ccatgaccac aaaacgcccc gtgatcacgg cccgcaccgt gtcgaggtca
     3481 atgtgctgct tggcggtgtc gcaggtgagc tccggcggaa tcttcttgac gcgtgcgagt
     3541 cc
//
LOCUS       DBNGZE010000314         2718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1572563, whole genome shotgun sequence.
ACCESSION   DBNGZE010000314 DBNGZE010000000
VERSION     DBNGZE010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1572563"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..417
                     /locus_tag="AC16FV_08175"
     CDS             79..417
                     /locus_tag="AC16FV_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR2850068.1"
                     /translation="MKKIEAIIKPFKLDDVREALSEAGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIELVVDDKMLDRCIEAITGAARTGKIGDGKIFVTPVEQVVRI
                     RTGEQGPEAI"
     gene            complement(428..1210)
                     /locus_tag="AC16FV_08180"
     CDS             complement(428..1210)
                     /locus_tag="AC16FV_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR2850069.1"
                     /translation="MRCATGLLTGLFLLLIAGCAGVTEDPTKDWSPEKFYTEAKAKVA
                     DGDYETGIKYFETLEARYPYGRYAEQAQLEIAYANYKYEEPALAIAAADRFIRLHPTH
                     PNVDYAYYLKGLVNFHGEKGLVNWLLGSKDDLSDRDPKGALEAYNAFNEVVQRFPNSR
                     YAEDSRQRMAYLFEAQAKYETLVARFYYERAAYVAAVNRAKYALDNYPRTPSTEDALG
                     IMALSYKAMGMNDLLKDTLRILGRNFPNSRYLAEVGALTASR"
     gene            1289..2260
                     /gene="rluD"
                     /locus_tag="AC16FV_08185"
     CDS             1289..2260
                     /gene="rluD"
                     /locus_tag="AC16FV_08185"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008489820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HIR2850070.1"
                     /translation="MNQESRLSASIPSALAGQRLDQALPALFADITRSRLQQWIEDGR
                     VTLNGQVPRKRDKVKEGDAVEVRVPPPAESGWKAQALPLEIVHEDSELLVINKPPGLV
                     VHPGAGNPEGTLLNALIAHAPKLAKLPRAGIVHRLDKDTSGLLVVAKTEHARQNLIGQ
                     LQEHSVEREYLAIVNGVMVAGGTVEAPIGRHRTQRTRMAVSSQGKPAVSHYRVMKKYR
                     AHTLVQVRLESGRTHQIRVHMAHLHYPVVGDPVYGGRLKIPTGASEKLKEVLRGFKRQ
                     ALHALKLSLIHPKTGKRVQWATSVPEDLSKLMEALASDARAHSDHGV"
     gene            2333..>2718
                     /locus_tag="AC16FV_08190"
     CDS             2333..>2718
                     /locus_tag="AC16FV_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR2850071.1"
                     /translation="MAINTLEFVYPDWPAPVHVKAATTTRNGGFSVGPYASFNLSNLV
                     GDEPGSVKRNRALLRDVLKLPAEPIWLTQVHGSRVIDAFDAGPREEADGCVTGTGGVV
                     CAVLTADCLPVFLCDRRSTRIAALHAG"
BASE COUNT          541 a          805 c          869 g          503 t
ORIGIN      
        1 cgttcttggc gtcttggcgg tttaatcctg ttttgttctg gtttaacgat ccgaaaaact
       61 gatccgaggt ttccgacaat gaaaaaaatc gaggccatca tcaaaccctt caaactcgac
      121 gacgtgcgcg aggctctgtc cgaggccggc gtgagcggcc tgaccgtgac cgaggtgaaa
      181 ggcttcggcc ggcagaaggg gcacacggag ctctaccgcg gcgcggaata cgtggtggat
      241 ttcctgccca aggtaaaaat cgaactggtg gtggacgaca agatgctgga tcgctgcatc
      301 gaggccatca ccggcgcggc gcgcaccggc aagatcggtg atggcaagat ttttgtcacg
      361 cccgtcgaac aggtggtgcg tatccgcacc ggcgagcagg ggccggaggc gatttaaccg
      421 ccgtagacta acggcttgcc gtcagtgcgc cgacctcggc gagataacgg ctgttcggaa
      481 agttcctgcc gagtatgcgc agcgtgtctt tcagcaggtc attcatcccc atcgccttgt
      541 aggacagggc catgatcccg agagcgtctt ccgtcgaggg cgtgcgcgga tagttgtcca
      601 gcgcgtattt ggcgcgattg acggccgcga cataggcggc gcgttcgtag taaaagcgcg
      661 ccaccagcgt ttcgtacttc gcctgggcct cgaacagata ggccatgcgt tgccgcgaat
      721 cctcggcgta gcggctgttg ggaaagcgct gcacaacttc attgaaggcg ttgtaggcct
      781 cgagcgcgcc cttggggtcg cggtcggaga gatcgtcctt ggagcccagc agccagttca
      841 ccagcccctt ctcgccgtgg aaattcacca ggcccttgag gtaatacgcg taatccacgt
      901 tcggatgcgt cgggtgcaga cggatgaagc gatcggcggc cgcgatggcg agcgccggtt
      961 cctcgtattt atagttggcg taggcgattt ccagttgcgc ctgttcggca tagcgtccgt
     1021 aaggatagcg cgcctcgagg gtttcaaaat atttgatgcc ggtctcgtaa tcgccgtccg
     1081 ccaccttggc cttggcctcg gtgtagaatt tctccggcga ccagtccttg gtcgggtctt
     1141 cggtgacgcc ggcgcatccg gcgatcagca gcaggaaaag tccggtcagc agcccggtgg
     1201 cacaacgcat tcgatttacc tcgatacaac gtataaagtc gcgccagctt aacccgattt
     1261 cacttcgccg gaaagaccga tcacaggcat gaaccaggaa tccagactgt cggcaagcat
     1321 tccatccgcg ctggccgggc aacggctcga tcaggcgttg ccggcgctgt tcgcggacat
     1381 tacgcgttca cggctgcaac agtggattga agacgggcgc gtcaccctca acggccaggt
     1441 tccacgcaag cgcgacaagg tgaaagaggg cgacgcggtg gaggtcaggg tcccgccacc
     1501 agcggaatcg ggctggaagg cgcaggcgct tccgctggag atcgtgcacg aagacagcga
     1561 gctgctcgtc atcaacaaac cacccggcct ggtggtgcat ccgggcgcgg gcaatccgga
     1621 aggcaccttg ttgaatgcgc tgatcgcgca cgcgccgaaa ctcgcaaagc tgccgcgcgc
     1681 cggcatcgtg caccggctcg acaaggatac ctcggggctg ctcgtggtcg ccaagaccga
     1741 gcacgcccgc cagaatctga tcggacagtt gcaggaacac agtgtcgagc gcgagtatct
     1801 tgccatcgtg aacggtgtca tggtggcggg cgggaccgtc gaggccccga tcggacgcca
     1861 tcgcacccag cgcacacgca tggcggtgag cagccagggc aagcccgcgg tgagccatta
     1921 ccgcgtgatg aagaaatacc gcgcccacac gctggtgcag gtcaggctcg aatccggccg
     1981 cacgcaccag atacgcgtgc acatggcgca cctgcattat cccgtggtcg gcgatccggt
     2041 atacggcggc cggctcaaaa tccccactgg cgcgagcgaa aaactgaaag aagtattgcg
     2101 cggcttcaag cgccaggctc tgcatgcgct caaactgtcc ttgatccacc ccaaaacggg
     2161 gaaacgcgtg cagtgggcga cgtcggttcc tgaagacttg agcaaattga tggaggctct
     2221 ggcgtcggac gccagagccc attctgatca cggcgtttag cgtgttgtcg gtggcgacac
     2281 gtccatatcc cttgtaatat ctcccgccag cgggacagaa aaaccaaaga tggtggcaat
     2341 caatactctc gaattcgttt atcccgactg gccggcgccg gtgcacgtaa aggcggcgac
     2401 gaccacgcgc aacggcggtt tcagcgtcgg gccgtacgca tccttcaatc tcagcaatct
     2461 cgtgggcgat gagcccggta gcgtcaagcg caatcgtgcg ttgttgcgcg atgtgctgaa
     2521 gctcccggcc gagccgatat ggctcacgca ggtacacggc agccgcgtga tcgacgcctt
     2581 cgacgccgga ccccgcgagg aggcggacgg ttgcgtgacc ggcacgggcg gcgtggtgtg
     2641 cgcggtgctg acagcggatt gtctgccggt gttcctgtgc gaccggcgca gcacgcgcat
     2701 tgcagcgttg catgccgg
//
LOCUS       DBNGZE010000315         8705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_601174, whole genome shotgun sequence.
ACCESSION   DBNGZE010000315 DBNGZE010000000
VERSION     DBNGZE010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_601174"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1647)
                     /locus_tag="AC16FV_08195"
     CDS             complement(19..1647)
                     /locus_tag="AC16FV_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2850072.1"
                     /translation="MSTSPEQAGSGSSRPPTLTIPPAMGWQRKTYRLFLNRLSLPLLA
                     LALVFAGVGAEAYVILAYPKHANTLYPLLGLLMLAGLGLIWLIIARVRRDLVEPLSHL
                     RNWSLRMRGGNLSARIPVPESGEFRELAQDINRLSDELKSLTMEMDSHVRAQTVRLAR
                     KTQSLDILYDVASSLSQPGDLDKLLESFLDTFIELVDARAATVRILTDDGQTRLVASR
                     GLSAEVVEKDRTMDTSLCQCGWAAKEGGIRTQRGTHPCAKLLGMPMLKRDCQEFVVVP
                     VTYQDRLLGVYNLFLDRPRSALGEDIHDLLNSVGKHLGLALEKSRLDNQARQIAIMEE
                     RNIIGNELHDSLAQGLVSMRLQVKMLGESLHKKDLRASQSEVRHLRSAIDEAHDSLRE
                     LLANYRLKIDERGLVPAVAGMVERFSQETGINVYFHNECHKLNLTPLQESQIFHIVKE
                     ALSNIRKHSHACNARILLNNDSRGAYTLLIEDDGEGIAPVSEARPGEHIGLSVMRERA
                     ERLPGELTIESEPGEGTRIVLSFPVAPRQTAAAK"
     gene            complement(1810..2136)
                     /locus_tag="AC16FV_08200"
     CDS             complement(1810..2136)
                     /locus_tag="AC16FV_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR2850073.1"
                     /translation="MIKITPEAVTQVRKSAETSNAQSMALRIAVRREEDGTFVYGMGF
                     DDEGADDSHFVVEGINVLVSNSSKDLLTGATLDFVEINPGEHQFIFINPNDPAHAASP
                     PTGSQA"
     gene            complement(2182..3618)
                     /locus_tag="AC16FV_08205"
     CDS             complement(2182..3618)
                     /locus_tag="AC16FV_08205"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase CobB"
                     /protein_id="HIR2850074.1"
                     /translation="MNRLLISAAHKSSGKTTLSIGLCAALRQRGLAVQPFKKGPDFID
                     PMWLSLATGRDCHNLDFFLMGREEILRTFSARSEGADISLIEGNKGLYDGLSLDGSNS
                     NAALATLLQTPVVLVIDARGMTRGIAPLILGYQAFDKNIRIAGVILNQLGGSRHEAKL
                     RAVIEHYTNIPVLGAVHHDDRLTIVERHLGLMPSNEAAEARNKIDAIASLVAGSVDLD
                     RLLKIAAEAPALASPSPPLPLPLAGEGSEPSALRATPIRLGIARDAAFAFYYPGDLEA
                     LRAAGAELVPINTLQDKKLPEVDALLIGGGFPEVFMEQLEANTSLRHQIRLAIETGLP
                     AYAECGGLMYLSRSLSWKGKRCEMAGVIPGDVVMHDRPMGRGYVILRETGRGPWPATG
                     AGQTPAEIRAHEFHYSSLENLAPDTVYAYNVLRGTGIDGKHDGIVHKNLFACYAHLRD
                     LENNHWAARFVDFVRQHRRTGVKQIHAH"
     gene            complement(3742..4950)
                     /gene="nrfD"
                     /locus_tag="AC16FV_08210"
     CDS             complement(3742..4950)
                     /gene="nrfD"
                     /locus_tag="AC16FV_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2850075.1"
                     /translation="MAHAHFNTIEGRSPGFYGLLAILGAFLAAGGLAALYMEHNGHWV
                     TGMSNQIVWGTPHVFAVFLIVAASGALNVASIASVFGRQLYKPLTRLSGLLAITLLAG
                     GLAVLVLDLGRPDRLLEAMLYYNFKSIFAWNIILYTGFFAVVVIYLWMMMERRMNDYT
                     KPAGMLAFIWRLTLTTGTGSIFGFLVARDAYDTAVIAPMFIIMSFSYGLAIFLVVLMA
                     AMRWTGRELGDVVFGRLRNLLGVFVAAVLYFVTVYHLTNLYITQRHGIEGFILLHGGQ
                     FTQVFWIGQIVLGSVVPLVILFSRIGQSRAMVVLASLLVVLGGLAQMYVTIIGGQAYP
                     MPLVAGKEISSSFFDGVVASYVPSLPEAVLGIGGIALALALVVVALKVLPFLPRSLSD
                     ADADPHYKAA"
     gene            complement(4954..5679)
                     /gene="dsrO"
                     /locus_tag="AC16FV_08215"
     CDS             complement(4954..5679)
                     /gene="dsrO"
                     /locus_tag="AC16FV_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrO"
                     /protein_id="HIR2850076.1"
                     /translation="MSLSRREFIGVGSAAVAGMALAPGLRLVELAQARSPDEAVTSLQ
                     RWGLLVDISKCNDCDACVTACSTENGWYGHNRPASDPQWIRKVTLKDKQTGYVQTLPV
                     MCQHCESPPCVDVCPTGASFKRADGIVLVDKHICIGCRYCMMACPYKARSFVHEETTD
                     QKPYAPRGKGTVESCTLCVHRVDAGKIPACVEACNKDGNRAMVFGDLKDPNSDIRKHL
                     ASGASTQLRPDLNLNTGVRYRGI"
     gene            complement(5676..5993)
                     /locus_tag="AC16FV_08220"
     CDS             complement(5676..5993)
                     /locus_tag="AC16FV_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850077.1"
                     /translation="MKIHRGEKCVEPTDDMRRNHMKYILHQRDKTMHQGIRTTKHSLK
                     NCVDCHADPATNSVLGQDGFCSSCHQYASVQIDCFSCHTDKRDKNAVAAVPATNAIKG
                     TQP"
     gene            complement(6108..8111)
                     /locus_tag="AC16FV_08225"
     CDS             complement(6108..8111)
                     /locus_tag="AC16FV_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR2850078.1"
                     /translation="MATPADKAKEVKAGFTFRKFKDGDQEWPSIQSRIFTADETDKCP
                     TYVHKTPPCQASCPSGEDIRGWLNIVRGIEKPPKDVKWQEYAFRRSTDANPFPSMMGR
                     VCPAPCQTGCNRNVVEDYVGINAVEQFIGDWALQNNVAFKPAEQSTGKKVAIIGGGPA
                     GLAAAYQLRRKGHAVTLFDDHDELGGMAKYGVPGYRLPRESLDGEINRIINMGVEVKL
                     KTRVGVDVPMKDVEKNFDAVLFAIGCKSGRALPVPGANAPNCITGVAFLEAFNQGRLK
                     AVSGRVVVIGGGDTSIDVVSVARRLGYIKEMAETERPEYIVMGQTAHDVAVTAKKDGA
                     DVLLISRQPVEKMNAAKHEIEDAQREGVVIRGSLQPKEVILDDKGRAKALRVVELDFS
                     ANPPVEKPGTEKDLEADLIVSAIGQMGDFRGMEEFNNGKGLINSDKFYQVPGKPGHFV
                     CGDIIRPHLLTTVIGQGSIAADSIDHYLKKQEMSKRPKVDVHHFNLLAKLNEVGLTPQ
                     QFKPAGPEDERPIDRGLRGTSEQGKAVGGYAIHNYEDRADAEVCQSNELFLGHFQYTP
                     RLKRTEMVPDAKEVLGHFKERMLGLDEKQTVEEAKRCMSCGMCFECDNCVIYCPQTAV
                     KRTPKSESTMGRYVYTDYDLCIGCHICADVCPTGYIKMGMGDH"
     gene            complement(8151..>8705)
                     /locus_tag="AC16FV_08230"
     CDS             complement(8151..>8705)
                     /locus_tag="AC16FV_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR2850079.1"
                     /translation="RVAYSFWNTLAGPFDFLDPRYPVPQHICEFTYDLIQKGALKFDK
                     SANDDKVVTFHDSCNVARASRMGNMPGGQFIIPRAVVKASVNKFVDMAPETIYESTYC
                     CGGGGGLLTDELLDLRIKGSAPRMQALKEVVEEHGVTHIAAICAICKAQFTKILPYYQ
                     FPMDMIQSTHGLVSKAIRLGPVNE"
BASE COUNT         1578 a         2554 c         2807 g         1766 t
ORIGIN      
        1 gtttcgtctt gttctctgtt attttgccgc ggccgtctgg cgcggcgcga ccgggaacga
       61 taacacaata cgggtgcctt cgccgggttc gctttcgatg gtgagctcgc ccgggagacg
      121 ttccgcgcgc tcgcgcatga cggaaagccc gatgtgttcg ccggggcgtg cctcggaaac
      181 gggggcaatg ccttcgccat cgtcctcgat cagcagggta taggccccgc gactgtcgtt
      241 gttgagcaga atgcgggcgt tgcaggcatg gctgtgtttg cggatgttcg atagtgcttc
      301 tttgacgatg tgaaaaatct ggctttcctg gagcggcgtc aggttgagct tgtggcattc
      361 attgtggaaa tacacattga tgccggtctc ctggctgaag cgctccacca tgccggccac
      421 ggccggtacc agaccacgct catctatttt caggcggtag ttggccagca gttcgcgcag
      481 gctgtcatgc gcctcgtcga tggcgctgcg cagatggcgt acctcgcttt gcgaggcgcg
      541 caggtccttc ttgtgcaggg actcgccaag cattttgacc tgcagccgca tgctgacgag
      601 tccttgcgcc agcgagtcgt gcaattcatt gccgatgatg ttgcgttctt ccatgatcgc
      661 gatttgtcgc gcctggttgt cgagccggga tttttcgagc gcgaggccga gatgcttgcc
      721 gacgctgttg agcaggtcgt ggatatcctc gcccagcgcc gaccttggcc ggtctagaaa
      781 caggttgtac acgccgagaa ggcggtcctg ataggtgacc gggacgacga cgaattcctg
      841 gcaatcgcgt ttcagcatcg gcatgcccag caacttggcg caggggtggg tgccgcgctg
      901 ggtgcggatg ccgccttcct tcgcggccca gccgcactga cacaggctgg tgtccatggt
      961 gcggtctttt tccaccacct ccgccgacag gccacgactg gcgaccaggc gtgtctggcc
     1021 atcgtcggtg agtatgcgca ccgtcgcggc gcgggcatcc accagctcga tgaaggtgtc
     1081 gagaaagctt tccagcagct tatcgaggtc gcccggctgg ctgaggctcg aagccacgtc
     1141 gtagagaata tccagcgact gggtcttgcg cgccaggcgc acggtctggg cgcgtacgtg
     1201 cgagtccatt tccatggtca gcgatttcag ctcgtcgctc aggcgattga tatcctgcgc
     1261 cagctcgcgg aactcgccgc tctcgggaac cgggatgcgc gccgagagat tgccgccacg
     1321 catgcgcagc gaccagttac gcaggtgcga cagtggctcc accagatcgc ggcgcacgcg
     1381 cgcgatgatc agccagatca gtccgagacc cgccaacatc agcaagccca acaacggata
     1441 aagggtgttg gcgtgctttg ggtaggccag aatgacatac gcttccgcgc ccacgccggc
     1501 aaaaaccagc gccaaggcga gcaaaggcaa tgacaggcga ttcaggaaaa gcctgtacgt
     1561 cttgcgctgc cagcccatgg cgggcggtat cgtcagggtg ggcggtcggg aagagccgga
     1621 ccctgcctgt tccggggaag tgctcatata cacgccgcat ccatgagcgg cggaaatcga
     1681 ttgaacatta atcagctaga ttagccttgg cgccggatgg cgtccagcca cttgcggtta
     1741 aacccgtata ttccgtgaga gtccaggtcc ggcagcgtat gcgactgccg ctgacaatga
     1801 cgaggcctgt tatgcctgac tgccggtggg aggcgaagcg gcgtgcgccg gatcgttggg
     1861 gttgatgaag ataaactggt gttcgcccgg gttgatctcg acaaaatcca gtgtcgcgcc
     1921 cgtcagcagg tccttgctgg aattggacac gagcacattg atgccctcca ccacgaaatg
     1981 cgagtcgtcg gcgccttcat cgtcaaagcc catgccgtag acgaacgtgc cgtcttcctc
     2041 gcggcgcacg gcgatgcgca acgccatgct ttgcgcgttg ctggtttcgg ccgacttgcg
     2101 cacctgtgtg acggcttccg gggtgatctt gatcataaat gactcctcga acggatattt
     2161 aatgatggcg cgccgggccc gtcaatgtgc gtgtatctgt ttgacgccgg tacgacgatg
     2221 ctggcgcacg aaatccacga agcgggcggc ccagtgattg ttctccagat cgcgcaggtg
     2281 cgcgtagcag gcgaacaggt tcttgtggac gatgccgtcg tgcttgccat cgatgccggt
     2341 gccgcgcagc acgttatagg catacaccgt atccggggca agattctcca gactggagta
     2401 gtgaaattcg tgcgcgcgga tttcggccgg tgtctgtccc gcgcctgtcg ccggccacgg
     2461 gccacggccg gtttcacgca ggatgacata gccgcgtccc atgggacggt cgtgcatcac
     2521 cacgtcgccc ggaatcactc ccgccatttc gcaacgcttg cctttccaag aaagactgcg
     2581 tgacaggtac atcagcccgc cgcattccgc atacgccggc aggccggtct caatggccag
     2641 acgaatctgg tgtcgcagtg acgtgttggc ttccagttgt tccatgaaaa cctcgggaaa
     2701 gccgccgccg atgagcaggg catccacctc cggcaacttc ttgtcctgca gtgtgttgat
     2761 cggcaccagt tccgcacccg cggcgcgcag cgcctcgaga tcgcccgggt aataaaaggc
     2821 gaaagcggca tcgcgcgcaa tgcccagtct tatcggagtc gcgcgcagcg cgctcggctc
     2881 gctcccctct cccgcaagcg ggagagggag tgggggtgag ggcgaagcga gtgccggtgc
     2941 ctcggcagct atcttcagca gccggtcgag atcgacagag ccggcgacca gggaggcgat
     3001 ggcgtcgatt ttgtttcttg cttcggccgc ctcgttactg ggcatcaggc cgagatggcg
     3061 ctcgacgatg gtcaggcggt catcgtgatg taccgcaccc agtaccggta tgttcgtgta
     3121 atgctcgatg acggcgcgca acttggcctc atgacgcgag ccgccgagct ggttgagaat
     3181 gacaccggca atgcggatgt tcttgtcgaa ggcctgataa cccaggatca gcggggcgat
     3241 gccacgggtc atgccgcgcg catcgatcac cagcaccacg ggggtttgca gcagggtggc
     3301 cagggccgcg ttgctgttgc tgccgtcgag gctcaaaccg tcgtacaggc ccttgttgcc
     3361 ctcgatcaag gagatgtcgg cgccctcgga tcgggccgaa aaggtgcgca ggatttcctc
     3421 gcgccccatg agaaagaaat cgagattgtg acaatcgcgg ccggtcgcca gcgacagcca
     3481 catgggatcg atgaagtccg gtcctttctt gaacggctgc acggcgaggc cgcgctggcg
     3541 cagggcggcg cacaggccga tgctcagcgt ggtcttgccg gaggatttgt gcgcggcgga
     3601 gatgagcagc ctgttcatgc ggtcaagtat gaacttttgc cgcaaaggcg caaagaacgc
     3661 aaagattcgc aggttttaaa accgaatcct ttcctctttg cgttttcttt gcgggctctg
     3721 cgtctttgcg gcgaagaact attaagcagc cttgtaatgc ggatcggcgt cggcgtccga
     3781 caggctgcgc ggcaggaagg ggagcacctt cagggccacg accaccaagg ccagggccag
     3841 ggcgataccg ccgataccca gaaccgcctc gggcaggctg gggacgtaac tggcgacgac
     3901 gccgtcgaag aagctgctgc tgatttcctt gcccgccacg agcggcatgg gataggcctg
     3961 accgccgatg atggtgacat acatctgggc caggccgccg aggacgacga gcagcgaagc
     4021 gagcaccacc atggcgcgtg attggccgat acgcgagaac aggatgacca gcgggaccac
     4081 gctgccgaga acgatctggc cgatccagaa cacctgcgtg aactggccgc cgtgcagcag
     4141 gatgaagccc tcgatgccat ggcgctgcgt gatgtaaaga tttgtcaggt gatagacggt
     4201 gacaaaataa agcacggcgg cgacgaacac gccgagcagg ttgcgcagcc gtccgaacac
     4261 tacgtcgccc agctcgcgcc cggtccagcg catggccgcc atcaacacga ccaggaatat
     4321 ggccaagccg taggagaagg acatgatgat gaacatgggg gcgatcacgg cggtgtcgta
     4381 ggcgtcgcgc gccacgagga agccgaagat ggagccggta ccggtggtga gcgtgagacg
     4441 ccagatgaag gcgagcatcc cggccggttt ggtgtagtca ttcatccgtc gttccatcat
     4501 catccacaga taaatgacga ctacggcgaa gaaaccggtg tacagaatga tgttccacgc
     4561 gaagatggat ttgaagttgt aatagagcat ggcctcgagc agccggtcgg gccgccccag
     4621 atcgagcacc agcaccgcca gaccgccggc aagcagggtg atggccagca ggcccgaaag
     4681 ccgggtcagg ggtttataaa gctggcggcc gaaaaccgag gcgatcgagg cgacgttgag
     4741 cgcgcctgag gctgctacta tgaggaacac cgcgaagaca tgtggcgtgc cccacacgat
     4801 ctggttggac atgccggtga cccagtgacc gttgtgttcc atgtataaag cggccagacc
     4861 gccggcggcc aaaaaagcgc cgagaatggc aagcagaccg taaaaacccg ggctgcgccc
     4921 ttctatcgta ttgaagtgtg catgtgccat gcgtcagatc ccccggtaac gcacgccagt
     4981 gttgagattc aggtccgggc gcagttgagt ggatgctccc gaggccagat gtttgcggat
     5041 gtcgctgttc ggatccttga ggtcgccgaa caccatcgcc ctgttgccgt ctttgttgca
     5101 ggcctcgacg caggccggga tcttcccggc atcgacgcga tgcacgcaca gcgtgcagga
     5161 ctcgaccgtg cccttgccgc gcggcgcgta cggtttctga tcggtcgttt cttcgtgcac
     5221 gaacgagcgc gccttgtacg ggcaggccat catgcagtat cggcagccga tgcagatatg
     5281 cttgtcgacc agaacgatgc cgtcggcgcg cttgaacgag gcgccggtgg gacaaacgtc
     5341 cacgcacggc ggtgattcgc agtgctgaca catgaccggc agggtttgca cgtagccggt
     5401 ctgtttgtcc ttgagggtga ccttgcggat ccactgtgga tcgctggccg ggcggttgtg
     5461 tccgtaccag ccgttttccg tgctgcaggc ggtcacacaa gcgtcgcagt cgttgcactt
     5521 gctgatgtcg acgagcaggc cccagcgctg caggctggtg acggcctcat cgggtgagcg
     5581 ggcctgcgcc agttccacca aacgcagtcc gggggccagc gccatgccgg cgaccgcggc
     5641 gctgccgact ccgatgaatt cgcggcggct gaggctcatg gttgcgttcc cttgatggcg
     5701 ttcgttgccg ggacggcggc aacggcgttc ttgtcgcgct tgtcggtatg acagctaaag
     5761 cagtcgatct gcaccgaagc gtattgatga caggaggaac agaagccatc ctggcccagc
     5821 acgctgttgg tggcgggatc ggcatggcaa tcgacgcagt ttttcaggct gtgtttcgtg
     5881 gtgcggatgc cctgatgcat cgtcttgtcg cgctggtgca ggatgtactt catgtggttg
     5941 cgccgcatgt cgtcggttgg ttccacgcac ttctcgccgc ggtgaatttt cacggtcggg
     6001 ccgatgctcg caggtttttt cgcttcagca gcgaaagcca ctgatgtcgc aagctgaccg
     6061 gcgattatgc caacaaggag tcttttcatc gcacctttca ctgtcgttca gtgatcgccc
     6121 atgcccatct tgatgtagcc ggtggggcag acgtcggcgc agatgtggca accgatgcac
     6181 aggtcgtaat cggtgtacac gtagcgtccc atggtcgatt ccgacttggg cgtgcgcttg
     6241 accgccgttt gcgggcagta aatgacgcag ttgtcgcact cgaagcacat gccgcagctc
     6301 atgcagcgct tggcttcttc cacggtctgt ttctcgtcca ggccgagcat gcgttccttg
     6361 aagtggccca gcacctcctt ggcgtcgggc accatctcgg tgcgcttcag tcgcggcgtg
     6421 tactggaagt gcccgaggaa cagttcgttc gactggcaca cctcggcgtc ggcgcgatcc
     6481 tcgtagttgt ggatcgcgta gccaccgacg gccttgccct gttccgaagt gccgcgcagg
     6541 ccgcgatcga tcgggcgctc gtcttccggg ccggccggct tgaattgctg cggcgtcaga
     6601 ccgacctcgt tgagcttggc gagcaggttg aagtgatgca cgtccacctt cggacgcttg
     6661 ctcatttcct gtttcttcag gtaatggtcg atgctgtcag cggcgatcga accctgaccg
     6721 atcacggtgg tgagcaggtg tgggcgaatg atgtcgccgc acacgaagtg gccgggtttg
     6781 ccaggcacct gatagaactt gtcggaattg atcagaccct tgccgttgtt gaattcctcc
     6841 atgccgcgga aatcgcccat ctggccgatg gcggaaacga tcagatcggc ctccaggtcc
     6901 ttttccgtgc ccggtttttc caccggcggg ttggctgaaa agtcgagttc caccacacgc
     6961 aacgccttgg cgcggccctt gtcgtcgaga atgacttcct ttggttgcag cgagccgcgg
     7021 atgactacgc cttcgcgctg ggcatcctcg atctcgtgct tggcggcgtt catcttttcc
     7081 accggctggc gcgagatcag caggacgtcg gcgccgtctt ttttggccgt gacggcgacg
     7141 tcgtgggcag tctgtcccat gacgatatat tccggacgct cggtctcggc catttccttg
     7201 atgtaaccca ggcggcgcgc caccgacacc acgtcgatgg aggtgtcgcc gccgccgatc
     7261 acgaccacgc ggccggacac ggccttgagg cggccctggt tgaaggcctc gaggaaggcg
     7321 acaccggtga tgcagttcgg ggcgttggcg ccgggaacgg gcagggcgcg gccggacttg
     7381 caaccgatgg cgaacaggac ggcgtcaaaa ttcttttcca cgtccttcat cggcacgtcc
     7441 acgccgacgc gggtcttgag cttgacctcg acgcccatat taatgatgcg attgatttca
     7501 ccgtccagcg actcgcgcgg caggcgataa ccgggcacac cgtacttagc catgccgccg
     7561 agctcgtcgt ggtcgtcgaa cagcgtgacc gcgtggccct tgcggcgcag ttggtaggcc
     7621 gccgcgagac ccgccggtcc gccgccgatg atggcgactt ttttgccggt ggactgttcc
     7681 gccggcttga acgcgacgtt gttttgcagc gcccagtcac cgatgaattg ctcgacggca
     7741 ttgatgccga cgtaatcttc caccacgttg cggttgcagc cggtctggca cggcgccggg
     7801 cacacgcggc ccatcatgga tgggaagggg ttggcgtcgg tggagcgacg gaaggcgtac
     7861 tcctgccact tcacgtcctt cggcggcttc tcgatgccgc gcacgatatt gagccagccg
     7921 cggatgtctt cacccgaggg gcagctcgcc tggcacggcg gtgtcttgtg cacgtaggtc
     7981 gggcacttgt cggtttcgtc ggccgtaaaa atgcgcgact gtatggaggg ccattcctgg
     8041 tcgccgtcct tgaacttgcg gaaggtgaaa ccggccttga cctcttttgc cttgtctgcg
     8101 ggagttgcca ttgcgaatct cctgtctagc tactgtttgc gtgtaaaaga ctattcgtta
     8161 accgggccca accggattgc tttgctcacc agcccgtggg tgctctgaat catgtccatg
     8221 gggaactgat agtagggcag aatcttggtg aactgcgcct tgcagatggc gcagatcgcg
     8281 gcgatgtgcg tcacgccgtg ttcttcgacg acttccttga gcgcctgcat gcgcggggct
     8341 gagcccttga tgcgcaggtc cagaagttcg tcggtcagaa gtccgccacc gccgccgcag
     8401 cagtaggtgc tttcgtaaat ggtttcggga gccatgtcca cgaacttgtt gaccgaggcc
     8461 ttgaccacgg cgcgcgggat gatgaactgg ccgcccggca tgttgcccat gcgcgaggcg
     8521 cgcgcgacgt tgcacgagtc atggaaggtg acgaccttgt cgtcgttggc actcttgtcg
     8581 aacttgagcg cgcctttctg gatcaggtcg taggtgaact cgcaaatgtg ctgcggcacc
     8641 gggtagcgcg gatcgaggaa gtcgaacgga ccggccagcg tgttccagaa gctgtaggcc
     8701 acgcg
//
LOCUS       DBNGZE010000316         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_392100, whole genome shotgun sequence.
ACCESSION   DBNGZE010000316 DBNGZE010000000
VERSION     DBNGZE010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_392100"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..441
                     /locus_tag="AC16FV_08235"
     CDS             226..441
                     /locus_tag="AC16FV_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020553.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR2850080.1"
                     /translation="MGHQPITPDMTVWSVYRRYPQTLDVFFSFGCPDIRKGLFPLAMR
                     FMKLKWAARAHKVPVEDMIRALNAAVR"
     gene            503..1147
                     /locus_tag="AC16FV_08240"
     CDS             503..1147
                     /locus_tag="AC16FV_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR2850081.1"
                     /translation="MTDADRAFFEQEITDLLDRLYGTALRLAKNRADAEDLVAETVTK
                     AWANFGSLHDRHCFRGWLFRILTNTFLSECRKTVPTSLEALAEAQGEPDEEPAFSLFD
                     RLHQPFLLWWGNPEQEFLNKMLQQDLQKAVDALPEVFRVPLILCDLQGLNYQEIAETL
                     GVPVGTVRSRLNRGRSQLQKALWQHAQEAGLRHAPAADKESFPISQTGEHHERH"
     gene            1134..1373
                     /locus_tag="AC16FV_08245"
     CDS             1134..1373
                     /locus_tag="AC16FV_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2850082.1"
                     /translation="MSDIKEIGCEEALRRLFEYLDHELDGARHAEMEQHLHTCRACYS
                     RAEFERHLKGKLGAVGKEPAPPEFELRIKNLLRRF"
     gene            1393..>2171
                     /locus_tag="AC16FV_08250"
     CDS             1393..>2171
                     /locus_tag="AC16FV_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_391899561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR2850083.1"
                     /translation="MVAIVSNKREAILKAVEDMYTEVANCPMRSFHFPTGRHAAELVG
                     YPRAELNALPATTVESFAGVGYPFAAGVIREGDTVLDIGSGSGTDALLAARRAGPKGK
                     VYALDLTEAMRAKLARNIAVAGATNIEILAGNAEQIPLPDQSVDAVTSNGVLNLVPDK
                     MRAIREILRVLKPGGRLQISDIALARPITGKAKRDPKLWAECIVGAVEEDAYLDLFRE
                     TGFKHVEHVSSFDYFAHANGEDTRLMAGYFGAHAITFRARRP"
BASE COUNT          437 a          711 c          643 g          380 t
ORIGIN      
        1 ttatttacaa cacgctgttc gaagcgagag cggccagcgc ttgagcaggc gtctaaccat
       61 cggttgcacg ggacgccgtc gctgcgcgcc gccgcccgtg aaccgaaacg ttatcaaggg
      121 cgcattcaac cgggaaccct ccggcctcct gatgcgtcct ggtgtcaaca atacccgacc
      181 gtcgtaaact acagcaacag gcttccacta tggagaaggc gtgacatggg ccatcaaccc
      241 atcaccccgg acatgaccgt atggagcgtc taccgtcgct atccgcagac gctggatgtg
      301 tttttctcct tcggctgccc ggacatacgc aagggcctgt ttccgctcgc aatgcggttc
      361 atgaagctca aatgggccgc acgggcacac aaggttccgg tcgaggatat gatccgggcc
      421 cttaatgccg ctgtaaggtg acgccgggaa ccttgcgccc ggcatgaacg tccttgcgga
      481 gaatgaccca aagccattga ccatgaccga cgccgaccgc gccttcttcg agcaagagat
      541 taccgatctc ctcgaccgtc tctatggcac ggctctgcgg ctggccaaaa accgcgccga
      601 cgccgaggac ctcgtggccg aaacagtcac caaagcctgg gcaaatttcg gatcgttgca
      661 cgaccgccac tgttttcgcg gctggctgtt ccgcattctc accaacacat tcctgagtga
      721 atgtcgcaag accgtaccga cctcgctcga ggcgctggcc gaagctcaag gcgaaccgga
      781 cgaggaaccg gcgttctcgc tgttcgaccg gttacaccag ccgtttctct tgtggtgggg
      841 caatcccgaa caggagtttc tcaacaagat gctgcaacaa gacttgcaaa aagcagtgga
      901 tgccctgccc gaggtgtttc gggtgccgct aatcctttgc gacctgcagg gcctgaacta
      961 tcaggagatc gccgagacgc tcggcgtccc ggtcggcacc gtgcgctcgc gcttgaaccg
     1021 cgggcgctca cagttgcaaa aggcgctgtg gcaacacgcg caggaggccg gcctcaggca
     1081 tgctcccgcc gcggacaaag agtcattccc tatatcgcag acaggtgaac accatgagcg
     1141 acattaaaga gatcggttgc gaagaggccc tgcgccgctt gttcgagtac ctcgaccacg
     1201 agctcgatgg cgcgcgccat gccgaaatgg agcagcacct gcacacctgt cgcgcctgct
     1261 actcgcgcgc cgagttcgaa cgccacctca agggcaagct cggtgcggtc gggaaggaac
     1321 cggccccgcc cgagttcgag ctgcgtatca aaaacctgct gcgccggttc tgaaaaccgc
     1381 gaggaggatg acatggtcgc catcgtcagc aacaaacgcg aggccatcct gaaggccgtg
     1441 gaggacatgt ataccgaggt cgccaactgc cccatgcgct cctttcattt cccgacgggg
     1501 cgccatgcgg ccgagctcgt cggctacccg cgggccgagc tcaacgcact gccagccacg
     1561 acggtcgagt ccttcgccgg cgtaggttat ccgttcgccg ccggcgtcat ccgcgagggc
     1621 gataccgtgc tcgacatcgg cagcggctcg ggcaccgatg cgctcctcgc cgcgcggcgt
     1681 gcgggtccga agggaaaggt atatgccctg gatctcaccg aggcgatgcg cgcgaagctc
     1741 gcgcgaaaca tcgccgtggc gggcgccacc aacatcgaga ttcttgccgg caatgccgag
     1801 cagattccat tgccggacca gtcggtggac gcggtcacga gcaacggcgt gctgaatctg
     1861 gtgccggaca agatgcgcgc gatccgcgaa atcctgcgtg tactcaaacc cggcgggcgt
     1921 ctccagatca gcgacatcgc ccttgccagg ccgatcaccg gcaaggccaa gcgcgatccc
     1981 aaactctggg ccgagtgcat cgtgggcgcg gtcgaggaag atgcctatct cgacctgttc
     2041 cgggagaccg gcttcaagca cgtcgaacac gtttccagtt tcgattattt cgcccacgcg
     2101 aacggtgagg acacccgcct catggctggc tatttcggcg cccacgcgat caccttccgc
     2161 gcgcgccgcc c
//
LOCUS       DBNGZE010000317         1785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1082152, whole genome shotgun sequence.
ACCESSION   DBNGZE010000317 DBNGZE010000000
VERSION     DBNGZE010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1082152"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="AC16FV_08255"
     CDS             <1..239
                     /locus_tag="AC16FV_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2850084.1"
                     /translation="DTQDPQQDKNARPCLSRVFDLVLKLGGTLSGEHGIGVEKRDYIN
                     SAIDPVTLDLMRAIKRQFDPRGILNPDKIFPPLK"
     gene            374..1093
                     /locus_tag="AC16FV_08260"
     CDS             374..1093
                     /locus_tag="AC16FV_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850085.1"
                     /translation="MKHAFAFLAVSLAIIPAAEAASDIDQINQLIQADFRRLSEDLGA
                     ALSYKAVIPAKPLGLTGFDLGVEVTATKLQNRDAWDRASSNTAPDTLYIPKLHLHKGL
                     PLGFDIGAFYTSVPSSNIKLWGAELRYAIIEGGAVTPALGLRGTYSKLSGVDQLDFHT
                     RGLELLVSKGFTVFTPYAGVGRVWTVSEPVGVTNVSKEEFTQGKYFVGGNVNLGLVNL
                     ALEADKTGDAASYNAKLGFRF"
     gene            complement(1097..>1785)
                     /locus_tag="AC16FV_08265"
     CDS             complement(1097..>1785)
                     /locus_tag="AC16FV_08265"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR2850086.1"
                     /translation="VSSATDLRNMTTRFPILEAILSAGLLLAANVAVAVESVSLQALF
                     KDKAIILVDGARRVLKSGEESPEGVKLLVTDTQEEKAEIEIGGRRETLRLGVVTAGFA
                     SKGKGSVTLYPETNGHYFVDGLVNGAPVRFMVDTGATIIGMNSIVANRIGIDYRKQGR
                     PGFVNTAGGTVPTYYIKLNSVTVGDITLYNVDASIVEGSSPREALLGMSFLGHLNMKR
                     DSEKMELSER"
BASE COUNT          361 a          554 c          504 g          366 t
ORIGIN      
        1 acgacacgca ggacccgcag caggataaaa acgcgcgtcc ctgcctgtcg cgcgtgttcg
       61 atctggtcct gaaactggga ggcacgcttt ccggcgagca cggcatcggc gtggagaagc
      121 gcgattacat caacagtgcc atcgacccgg taacgctgga cctgatgcgc gccatcaagc
      181 gccagttcga tccgcggggg attttgaatc ccgacaaaat cttcccgccg ctgaagtaat
      241 tgcgaccgtt cttgctgccg cacgtttctt tcgggcggac aggcggtaag gtcccggacc
      301 gtgtttacct ttacgatgaa gccattgggc cagcggagaa tccggcccaa cccgcacgtc
      361 aaggagagat tttatgaaac acgcgtttgc gtttctggct gtctcgctgg cgattatccc
      421 cgcggccgag gcggctagcg atatcgacca gatcaatcag ttgatacagg cggacttccg
      481 ccggctgtcg gaagatctcg gcgcggcgct gagctacaag gccgtgatcc ctgccaaacc
      541 gctcggtctg accggcttcg atctcggcgt ggaggtcacg gccacgaagc ttcaaaaccg
      601 cgacgcctgg gaccgcgcca gttccaacac ggcgcccgac acgctgtaca tccccaagct
      661 gcatctgcac aagggcctgc cgctcggctt tgacatcgga gctttctaca cctcggttcc
      721 aagcagcaat atcaaactct ggggcgccga actgcgctac gcgatcatcg agggcggcgc
      781 cgtcacgcca gcgctcggtt tgcgcggtac atattcaaaa ctgtcgggcg tggaccagct
      841 cgatttccac accaggggtc tggagttgct cgtttccaag ggtttcaccg tctttacacc
      901 gtatgccggc gtggggcggg tatggacggt atccgaaccg gtgggtgtaa cgaacgtcag
      961 caaggaagaa ttcacccagg gaaaatattt tgtcggcggt aacgtcaatc tgggcctcgt
     1021 caatctcgcg ctcgaggccg acaaaaccgg tgatgccgct tcctacaacg ccaagctcgg
     1081 attccgcttc tgagcctcag cgctcgctca gctccatttt ctcgctgtca cgcttcatgt
     1141 tgaggtggcc gagaaaagac atgccgagca gcgcttcgcg cgggctagaa ccctcgacga
     1201 tgctggcatc cacgttatat aaagtgatat cgccgaccgt gacgctgttc agcttgatgt
     1261 agtacgtagg caccgtgcca cccgcggtgt tgacgaagcc aggacggccc tgcttacggt
     1321 aatcgatacc gatacggtta gccacgatgc tgttcatgcc gatgatggtc gcgccggtgt
     1381 ccaccatgaa gcgcacgggc gccccattca ccagaccatc gacaaagtaa tggccgttgg
     1441 tttcgggata gagcgtaacg ctgcccttgc ctttcgatgc gaatccggcg gtcaccacgc
     1501 ccagcctgag tgtttcgcgc ctgccgccga tttcgatctc ggccttttcc tcctgcgtgt
     1561 cggtgaccag cagcttgacg ccttccggac tctcctcgcc gcttttcagc acgcggcgcg
     1621 cgccgtccac aagaataatc gccttgtcct tgaacagagc ctggagggaa acgctctcca
     1681 ccgccacggc aacgttcgcc gccagcaaca gaccggcgga caatatcgcc tcaagtattg
     1741 gaaaacgcgt ggtcatattt cttaaatccg tcgctgacga tacaa
//
LOCUS       DBNGZE010000318         4772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_323641, whole genome shotgun sequence.
ACCESSION   DBNGZE010000318 DBNGZE010000000
VERSION     DBNGZE010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_323641"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2658
                     /locus_tag="AC16FV_08270"
     CDS             <1..2658
                     /locus_tag="AC16FV_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR2850087.1"
                     /translation="PGAERGANEFEAISWDEAFAMLEERLAKIRATDPKKFALFTGRD
                     QMQALTGLFAKQFGTPNYAAHGGFCSVNMAAGMIYTIGGSFWEFGGPDLDRAKLFVMI
                     GTAEDHHSNPLKIAISKFKRNGGKIISVNPVRTGYSAIADEWVPIKPGTDGALFLALI
                     HTLIKQGLYDRDFLVQYTNAAQLVNMDKNSSEYGMFVRAEVPVEEGCFDPQNKLWWDR
                     LTNKPVVTHSPGADPYLLGEFKLPDGTPVKPSFQLLVDRVRDYTPEWASAITGIPVAT
                     IKRLAHEMGVTARDQKIELPIAWTDIWGRDHETVTGNPVAFHAMRGLAAHSNGFHTIR
                     ALSILMSVLGTIDRPGGFRHKAPFPRPIPPCAKTPKGPEGVRPGKPLDGKALGWPADP
                     DDLFVDPDGAPVRIDKAFSWEYPLSAHGLMHNVITNAWRGDPYKLDTLLIFMANMAWN
                     SAMNTVEVRRMLNDKDENGEFKIPFIVVCDAFQSEMVSYADLVLPDTTYLERHDVMSM
                     LDRPISEFDGPVDSVRIPVVPPKGECKPFQEVLIELGSRLKLPAFVDNLGQRKFKNYP
                     DFIINYEIEPGAGIGFLAGWRGKGGEKFMKGEPNPNQWEMYAKNNCVYHHELPKSFQY
                     MRNWNKGYLEWAQRHRLTRYAEPILIHIYSDVLQQFRLAAQGKTHGKQPPQHLRKRIE
                     TYFDPLPFYYEPLETQLTDKQKYPLNAVTQRPMAMYHSWDSQNAWLRQIHTHNYLFVN
                     PKTAAAQGIEDGDWVWVESPWGKVRGMCRYSEATEPGTVWTWNAIGKQPGAWNLEPRA
                     NEARKGFLLNHLISEELPPRPEGEHLSNSDPVTGQAGWYDVRVRLYKAGPAEEKATWP
                     QFTPLPPVPGEPARRKNWLAYVAGKIRGD"
     gene            2794..3525
                     /locus_tag="AC16FV_08275"
     CDS             2794..3525
                     /locus_tag="AC16FV_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2850088.1"
                     /translation="MTQLALVIDLNVCVGCHACVTSCKEWNTSGAAGYMSDDRPYGED
                     PTGTFFNRVQTFEVGEFPNTETVHFPKSCLHCEDPPCVPVCPTGASYKRPEDGIVLVD
                     YDKCIGCKYCSWACPYGVREIDEHQKVMKKCTLCVDRIYDTALPEAQRKPACVLACPT
                     SARLFGDVHDPESAVSVAISENGGYALMSEWGTKPANNYLPRRKTRIKIHADELMRAD
                     NPLKKEGVQPKPDFREPSLDDVTSW"
     gene            3544..4521
                     /locus_tag="AC16FV_08280"
     CDS             3544..4521
                     /locus_tag="AC16FV_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HIR2850089.1"
                     /translation="MHPAFSVIFLTTLIGAGQGLFLALYTVQVYSTVELLPAQPNDFY
                     TLGSIISLVLLIAGLVASFFHLGHPERAWRSAARWRTSWLSREVIVLPALMGAVTVYG
                     LVHHFDLSLGIFGIETTLHGDLALVVGAGGVVLAFLLFLCTGMIYACIKFLQEWATWL
                     TVVNYTLFGLASGFLLATAYAAYRAPVLIYFYGAWTIIATLAVMVTRVASLIRNRRLK
                     HKSSLQTAIGVRHARIVQKSQGFMGGSFNTREFFHGATVWMFRSIKWIFLALVFPVTL
                     ALLFAGMIHGSAPLIASAFVVQYLGLLAERWFFFAQANHPQNLYYQTIS"
BASE COUNT          920 a         1458 c         1470 g          924 t
ORIGIN      
        1 ccgggcgccg aacgtggcgc caacgagttc gaggcgattt cctgggacga ggcgttcgcg
       61 atgctggaag aacgcctcgc gaaaatccgc gccaccgatc cgaaaaagtt cgcgctgttc
      121 accgggcgcg accagatgca ggcgctcacc ggcctgttcg ccaagcagtt cggcacgccc
      181 aactatgccg cgcacggcgg tttctgttcg gtgaatatgg ccgcgggcat gatctacacc
      241 atcggtgggt cgttctggga attcggcggg ccggatctcg accgtgccaa actgttcgtg
      301 atgatcggca ccgccgagga tcatcactcc aatccgctca agatcgccat ctccaagttc
      361 aaacgcaatg gcggcaagat catctccgtc aatccggtgc gcaccggtta ctcggcgatc
      421 gcggatgaat gggtgccgat caagcccggt accgatggcg cattgtttct cgctctgatt
      481 cacacgctca tcaagcaggg cctgtacgat cgcgacttcc tggtgcaata caccaatgcc
      541 gcgcagctgg tgaacatgga caaaaattca agcgaatacg gcatgttcgt gcgcgccgag
      601 gtgccggtgg aggagggctg cttcgatccg cagaacaagc tgtggtggga tcggctgacg
      661 aacaagcccg tggtcacgca tagccccggc gccgatccct atctgcttgg cgaattcaag
      721 ttgccggacg gcaccccggt gaagccgtcg ttccagttgc tggtggatcg cgtcagggac
      781 tacacccccg agtgggccag cgccatcacc ggcattccgg tcgcgacgat caagcgcctg
      841 gcgcacgaga tgggcgtgac ggcgcgcgac cagaagatcg agttgccgat agcctggacc
      901 gacatctggg gccgggacca cgagaccgtg accggcaatc cggtggcgtt tcacgccatg
      961 cgcggtctcg ccgcgcactc gaacggtttt cacaccatcc gcgcgctgtc gatattaatg
     1021 agcgtgctcg gcaccatcga ccgacccggc ggcttccgcc acaaggcgcc gttcccgcgc
     1081 ccgatcccgc cctgtgccaa gacccccaaa ggcccggaag gcgtgcgccc cggcaagccg
     1141 ctggacggca aggcgctcgg ctggccggct gacccggacg atctgttcgt cgacccggat
     1201 ggcgcgccgg tgcgcatcga caaggccttt tcctgggaat atccgctgtc ggcgcacggg
     1261 ctgatgcaca acgtcatcac caacgcctgg cgcggcgatc cgtacaagct cgacacgctg
     1321 ctgatcttca tggccaacat ggcgtggaac tcggccatga acacggtcga ggtgcgcagg
     1381 atgttgaacg acaaggacga gaacggcgag ttcaaaattc cgttcatcgt cgtgtgcgat
     1441 gcgttccagt cggaaatggt cagctacgcc gatctggtgc tgccagacac gacttacctc
     1501 gaacgccacg atgtcatgag catgctcgac cggccaatct ccgaattcga cggcccggtg
     1561 gattcggtgc gcatcccggt ggtgccaccc aagggcgagt gcaagccgtt ccaggaagtg
     1621 ctgatcgagc tcggttcgcg tctgaagttg ccggcgtttg tcgataatct cgggcagcgc
     1681 aagttcaaga actacccgga cttcattatc aattacgaaa tcgagccggg cgccggcatc
     1741 ggcttcctcg ccggctggcg cggcaagggt ggcgagaagt tcatgaaagg cgagcccaat
     1801 ccgaaccagt gggaaatgta cgccaagaac aactgcgtat accaccacga actgcccaag
     1861 tcgttccagt acatgcgcaa ctggaacaag ggttatctcg agtgggcgca gcgtcatcgt
     1921 ctgacgcgtt atgccgagcc gatcctgatc catatctatt ccgacgtgct gcagcagttc
     1981 cgcctcgccg cgcagggcaa gacccatggc aagcagccgc cgcaacatct gcgcaagcgc
     2041 attgaaacct atttcgatcc gctgccgttc tactacgaac cgctcgagac gcagctcacc
     2101 gacaagcaga aatatccgct caatgcggta acgcaacggc cgatggcgat gtatcactcg
     2161 tgggattcgc agaacgcctg gctgcgccag attcacacgc ataattatct gttcgtgaat
     2221 ccgaagacgg ccgcagccca aggcatcgag gacggcgact gggtatgggt cgagtcgccg
     2281 tggggcaagg tgcgcggcat gtgccgttac agcgaggcga ccgagcccgg cacggtctgg
     2341 acctggaacg ctatcggtaa acagcccggt gcatggaatc tcgaaccgcg cgcgaacgag
     2401 gcgcgcaagg gttttctgct caatcatctg atctccgagg aactgccgcc gcgtccggag
     2461 ggcgagcatc tgtccaattc cgatccggtg accggccagg ccggctggta cgacgtgcgc
     2521 gtgcgtctct acaaagccgg cccggcggaa gagaaagcga cttggccgca attcacgccg
     2581 ctgccgccag tgcccggtga gccggcgcgc cgtaaaaact ggctggccta cgtggccggc
     2641 aagatcaggg gagattagtc ttatgaaagg acgaacggag cgcagcggag tagggcgtcc
     2701 tcttatgaca ggacgaacgg agcgaatccg caaacgcgcc atccatggcg cgcggccctc
     2761 gcctcgatcc aatcggcgaa gcggagcagg gtcatgacac agctcgcgtt ggtgattgat
     2821 ctcaatgttt gcgtgggctg tcacgcctgc gtcacgagct gcaaggaatg gaacacctcg
     2881 ggcgcggccg gctatatgtc cgatgatcgt ccgtacggcg aagacccgac cggcactttt
     2941 ttcaaccggg tgcagacttt cgaggtgggc gagttcccca ataccgagac cgtgcatttc
     3001 ccgaaatcgt gcctgcattg cgaggacccg ccgtgcgtgc cggtgtgccc caccggcgcg
     3061 agctacaagc gcccggaaga cggcatagtg ctggtcgact acgacaagtg catcggctgt
     3121 aaatactgct cgtgggcctg cccgtacggc gtgcgcgaaa tcgacgagca ccagaaagtc
     3181 atgaagaagt gcacgctgtg cgtcgaccgt atttacgaca cggcgttgcc cgaggcgcag
     3241 cgcaagccgg cctgcgtgct cgcgtgcccg accagcgcgc gcctgttcgg cgacgtgcac
     3301 gatccggaat cggcagtgtc ggtcgcgatc agcgagaacg gcggttacgc gctcatgtcg
     3361 gagtggggca ccaagccggc caacaattat ctgccgcgcc gcaagacgcg catcaagatc
     3421 cacgccgacg aactcatgcg cgccgacaat ccgctcaaga aggaaggtgt gcagcccaag
     3481 ccggacttcc gcgagccgtc gctcgacgac gtcacgagct ggtaacgcta tcaggaacaa
     3541 cgcatgcatc ccgccttttc ggtcattttt ctgacgacgc tgatcggcgc tggccagggc
     3601 ctgtttctgg cgctgtacac cgtgcaggtt tattcaaccg tcgagctgtt gccggcgcag
     3661 ccaaacgact tctacacgct gggcagcatc atctcgctgg tcttactgat cgccgggctg
     3721 gtagcgtcgt tcttccatct cggccatccg gaacgcgcgt ggcgctcagc ggcgcgctgg
     3781 cgcacctcct ggctgtcgcg cgaggtaatt gtgctgccgg cattgatggg tgcggtaacg
     3841 gtctatggtt tggttcacca tttcgatttg agtctgggaa ttttcggcat cgagacgaca
     3901 ctgcacggcg atctggcgct cgtcgttggc gccggtggcg tggtgctggc gttcctgctg
     3961 tttctttgca ccggcatgat ctacgcttgc atcaagtttt tgcaggaatg ggcgacgtgg
     4021 ctgacggtcg tgaactacac gttgttcggg ctggcgtcgg ggttcctgct ggcgacggcc
     4081 tacgccgcgt accgggcgcc ggtgctgata tatttttacg gcgcctggac catcatcgcc
     4141 actctggcgg ttatggtgac gcgcgtggcc tcgctgattc gcaaccgccg gctgaaacac
     4201 aagtcgtcgc ttcaaactgc catcggcgtg cgccacgcgc gcatcgtcca gaagtcgcag
     4261 ggcttcatgg gtgggtcgtt caacacgcgc gagtttttcc acggcgcgac ggtgtggatg
     4321 ttccgctcga tcaaatggat attcctggcg ctggtatttc cggtaacgct tgccttgctg
     4381 tttgccggga tgatccacgg tagtgcgccg ctgattgcct cggcctttgt cgttcagtat
     4441 ttggggctgt tggcggaacg ctggttcttt ttcgcccagg ccaatcaccc ccagaatctg
     4501 tattaccaga cgatctcctg aatctgtcgg ttagatatca aggggatatc tcctccacat
     4561 tcgtcgggct ggatgcggca cttgttcacc acacgcggct aacacgctga cgcacaaggg
     4621 aatcaccgat catcccggtt aggccacctt gtatcggttc cggcttgtgc tttgggctcc
     4681 cccggccaag actttcttcc taagggacga tctgcgggca gaagggaatg acgggcatgc
     4741 agcccctctc gcagactgca tttcctgagg ag
//
LOCUS       DBNGZE010000319         7446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1919947, whole genome shotgun sequence.
ACCESSION   DBNGZE010000319 DBNGZE010000000
VERSION     DBNGZE010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1919947"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..729)
                     /gene="recR"
                     /locus_tag="AC16FV_08285"
     CDS             complement(133..729)
                     /gene="recR"
                     /locus_tag="AC16FV_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR2850090.1"
                     /translation="MNDITVLEALKQALRRLPGVGPKSASRMAYHLLERDREGARTIA
                     RALTDAVERIVHCKRCNNLSETELCPICSSNRRDKSLLCVVESPSDLVSLDQSGVYNG
                     MYFVLMGRLSPLDGIGPDEIGIPRLEALLDEGSIKEVVLATNLTVEGEATAHYISDLV
                     RARHIKATRIAYGVPIGGELEYTDRNTLARALAGRRET"
     gene            complement(734..1054)
                     /locus_tag="AC16FV_08290"
     CDS             complement(734..1054)
                     /locus_tag="AC16FV_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR2850091.1"
                     /translation="MKGGLGNIMKQAQAMQENLRKAQEQLATIEVTGSSGGGMVSVTM
                     TCRHDVKRVQIDPGLLQDDKEVLEDLIAAAMNDAARKVEKTTEEKMAGLTAGLNIPGM
                     KLPF"
     gene            complement(1072..1521)
                     /locus_tag="AC16FV_08295"
     CDS             complement(1072..1521)
                     /locus_tag="AC16FV_08295"
                     /inference="COORDINATES: protein motif:HMM:NF023592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HIR2850092.1"
                     /translation="MTTPASSVVAAPVAVSAPQSPGVTGTKWSEVVAQLGLTGLLREL
                     VNNTTQESHADGAMTLILDESHAKLFSKEREDELRKALEKHYGAPLRLKVNLGRPSAE
                     TPAKEKSRLQDERQQAAVQAIVEDPNVRALQEKFSARVNPASIRPKV"
     gene            complement(1588..2691)
                     /gene="dnaX"
                     /locus_tag="AC16FV_08300"
                     /pseudo
     CDS             complement(1588..2691)
                     /gene="dnaX"
                     /locus_tag="AC16FV_08300"
                     /inference="COORDINATES: protein motif:HMM:NF005942.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
     gene            complement(2739..2836)
                     /gene="ffs"
                     /locus_tag="AC16FV_08305"
     ncRNA           complement(2739..2836)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16FV_08305"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2966..6067)
                     /locus_tag="AC16FV_08310"
     CDS             complement(2966..6067)
                     /locus_tag="AC16FV_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2850093.1"
                     /translation="MKPLRLVMQAFGPYAARQEIDFRQLGGRSLFLIHGQTGAGKTSI
                     LDAICFALYGESSGAAREPRTLRSDHAPPDVPTEVSFDFSVGAERYRIKRMPEQERPK
                     KKGTGVTREPPEATLWRRSDMADDADDGTVLATQWIHVTEKVEQLLGFRCDQFRQVVM
                     LPQYQFQRFLMANSTERQRILEVLFSTEFYRQIEEALKQSAVDVRRRCEDLTGRSGFV
                     LAQAGVATAEELLDRQEALRRRAGEVEAGLTAAAARRELVDKQLRDAHQIDKVFVELT
                     EARARYARTEARRAEIDALDQRLEQARRAEALRDAEVYRQQRKQEHEAVLLKRTNFVT
                     QHERALEAKHAAERALAAEQAREAERELVRIEIARLEALTGKIGELEQGNTQLAEHSR
                     RVTQATHDRNRAQEELRECTRALRKHQKDLVRMIELSGKTAEWRAQVKELQRLHKIAA
                     DLAEHQGRLKMQEHALASAEALMRETKKSLNEAKKRFERLQEQRVKAQSTVLAKQLKR
                     GEPCPVCGSTRHPAPAHFRSAVPDEQEIAALQEELPRLEKTQDAAVQEAGQVVQRVAI
                     TKERIAKCLEDLGVHRKTSPAALAKQLEEAQAELDKAKAASEEQGDLRRTIAELQATE
                     TRLTEELPRLEEIFTEVSTAHGRLQERIAGLEKDIPATLRSLEALGAALAASQARHEA
                     LKTALEHARQNHDTAVAAWQACQAQLDGMEREVNETRERLEAVVEDFLKRLGEAGFVD
                     EEACQGARLEQEERDQLEQEIRTFRDAQVAAQARLDRVVDAARGKVRPDLAALETDAA
                     NAQAEVNGLLEERARTGEQSRQIDSSVGELKFLQGEQAQMEVQLAVVGRVAEVANGKN
                     IYGVTLQRYVLAALLEDVLAAASHRLRLMSRGRFDLQRATERVDLRIAGGLDLLVYDA
                     YTGTTRPVQTLSGGESCLASLSLAMGLADVVQSYAGGIRLETIFIDEGFDSLDSETLE
                     LAFQALGELLKSGRLVGLISHVAELTELVPARLEITKGKHGSHARLVHGETRIARHVA
                     AQA"
     gene            complement(6064..7215)
                     /locus_tag="AC16FV_08315"
     CDS             complement(6064..7215)
                     /locus_tag="AC16FV_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR2850094.1"
                     /translation="MRIIHTADWHLGRIFYGIHLTQDQAYVLDQFIKLVREAKPDAVV
                     IAGDVYDRAVPPPEAVSLLDETLSRLVIDLKTRVILIAGNHDSPERLGFGTRLLSQSG
                     LHVAGRVGLDPMWTSLTDNHGTVSVCAIPYAEPALVRERLGNPDLPDHAAAMRAVIAK
                     ARETRPRGTRSVLVAHAFVAGSEESESERPLSVGATGAVEPSVFDGFSFVALGHLHRP
                     QRAGNDRIHYSGSLLQYSFSEATHSKSVNLVELDAAGRCRLERIPLAPRRAVRCVTGT
                     LEALLNAPVGNDADDYLSVTLLDKGPVFDPIGRLRSKYPNVLELKRMERFGRGDPAAS
                     RVDYRNLSDREIFTSFFQTVKGEAASEDEINAYLGIVERLHALERESQS"
BASE COUNT         1263 a         2441 c         2265 g         1477 t
ORIGIN      
        1 ggcacgttcc agcatggcaa cgcgctcacc gatgtcacca caacctgcaa tcagcacatc
       61 agtggtggcg gggatcattg cgcaacgtca aacgtcatcg acgccagatg gcgccgattg
      121 atcatgaagt catcatgttt cgcgccggcc ggccaaggcg cgcgcgagcg tattgcggtc
      181 ggtgtattcc agctcgccgc cgatgggaac accgtaagcg atgcgcgtgg ccttgatatg
      241 acgggcgcgc accagatcgc tgatgtaatg cgcggttgcc tcgccctcga ccgtcaggtt
      301 ggtagcgagc acgacctcct tgatgctgcc ctcatcgagc agggcttcga ggcggggaat
      361 gccgatttcg tccgggccga tcccgtcgag cggcgacaac cgccccatga gcacgaaata
      421 catgccgttg tacacgccgg attggtcgag cgagacgaga tcggacggcg actcgaccac
      481 gcacagcagc gacttgtcgc gccggttcga cgaacagatc gggcacagct ccgtttcgct
      541 caggttattg cagcgcttgc aatgaacgat acgctcgacc gcatcggtaa gcgcccgcgc
      601 gatggtgcgc gcaccctcgc gatcgcgttc gagcagatga taggccatgc ggctggcact
      661 cttggggccg acaccgggca aacggcgcag ggcctgcttg agcgcttcga ggacggtaat
      721 gtcgttcatg acctcagaac ggcaatttca tcccggggat attcaatccg gcggtgagtc
      781 ccgccatttt ttcctcggtg gtcttctcga ccttgcgcgc cgcgtcattc atcgcggcgg
      841 cgatcaggtc ctccagcact tccttgtcgt cctgcaacag gccggggtca atctgtacgc
      901 gcttgacgtc atgccggcaa gtcatggtca cgctgaccat gccgcccccg ctgctgccgg
      961 tcacctcgat cgtcgccagt tgctcctggg ccttgcgcag attctcctgc atggcctgcg
     1021 cctgtttcat gatattgcct aatccacctt tcatgttttc ctcaccgtgc attaaacctt
     1081 gggtcgaatc gaagccgggt tgacccgggc gctgaatttc tcctgcaacg cgcgcacgtt
     1141 ggggtcttcg acaatggcct gcaccgccgc ctgctgccgt tcgtcctgca aacggctctt
     1201 ttctttcgcc ggcgtttccg cgctcgggcg acccagattc accttcagac gcagcggcgc
     1261 gccgtaatgt ttctccagcg ccttgcgcag ctcatcctcg cgctccttgc tgaacagctt
     1321 ggcgtgcgat tcatccagta tcagcgtcat ggcgccgtcc gcgtgcgatt cctgcgtcgt
     1381 gttgttgacc agttcgcgca acaggcccgt gagcccgagc tgggccacaa cttcgctcca
     1441 tttcgttccc gttacacccg ggctttgcgg ggccgatact gcgaccggag cggcaacgac
     1501 ggaacttgcc ggcgttgtca cgggcgacgc ggtccgtggc gccggcaccg atgccgccgg
     1561 cgttaccgtt ctgggcgcag acgcagcggc gacgggtgaa cgcacctcac ccgcttccgc
     1621 cggacggaac gccaccatgc gcaacaaaat catttccaat ccactgcgcg gatcgggggc
     1681 atagggcaga tcgcgttgtc ccatcacgcc aatctggtaa taaagctgca cgtcctgcgg
     1741 cgacatcgca cgggtatgcg caagcacgct ttcaccctct tcgcccgcca gcgccgcatc
     1801 ggcctgcgcc agtgcaatgc ggtgcagggc aagcaacagg tccttcagca gggcttcgta
     1861 atccaggccg aactcggtgg cctcggacac ggtcttgagc aacgcagcga catccccggc
     1921 ctccagtccc tgcaggatgg cctcgatgaa attctggtcg atcgtgccca gcatcgcgca
     1981 cacttccgcc tcattgacct gaccgccgcc ataggcaatt gcctgatcga gcagcgacag
     2041 gccgtcgcgc atgctgccat cggcggcgcg cgccagcagc ttgatggagg cggtgtcgtg
     2101 agtgatgccc tccgcgtcca gaatcttctg gatctgcgcg gcgatctgct ccaacggcag
     2161 acgtttcagg ttgaattgca ggcagcgcga cagcaccgtc accggcagtt tctgcgggtc
     2221 ggtggtggcg agcaggaatt tgacgtgcgg cggcggctct tccagcgtct tcagcagcgc
     2281 attgaagctg tggccggaga gcatgtgtac ttcgtcgatg agatagacct tgtagcggcc
     2341 gcgcgtcggg gcgtactgca cgttgtcgag cagttcgcgc gtgtcatcca ccttggtgcg
     2401 cgaggccgcg tccacctcga tcagatcgac aaaacggccc tcgtccactt ccttgcaggc
     2461 gctgcattgg ccgcacggct tcgaggtaac gccggtttca cagttcagtg atttcgccag
     2521 aatgcgggcg atggtggtct tgcccacgcc gcgggtgccg gtaaacagaa aggcatgatg
     2581 caatcggccg tggtccagcg cgttgaccag cggctgcacc acgtgttgct ggcccacgag
     2641 ttcggcgaaa ttacgcggtc gccatttacg cgctagtacc tgataactca tgaacttcgg
     2701 tttgctctca aaacggatgg tctaaaacgg gtggcggttc gagccaacca caccccagta
     2761 cccgaaatac ctgctgcggc tgcttccttc cggacctgac cgggttcaca agcttccgtc
     2821 gccgagggga ccggctctca ccgccataat caatcggtag gacgaaagtg taccacggcg
     2881 tgtgatggcg gtaacccaag gaagatgtcg cgccaatccc gaatgccccg caaaccactg
     2941 cggtcatggc gcgatgcggt ttgtttcagg cttgcgcagc gacgtgccgg gcgatgcgcg
     3001 tttcgccatg caccagccgt gcatggctgc catgcttgcc cttggtgatc tcaagccgag
     3061 ccgggaccag ctcggtaagc tcggcaacat gggagatgag tccgaccagg cgtccactct
     3121 tcagcaactc gcccagcgcc tggaaagcga gctcgagggt ttcggagtca agcgaatcga
     3181 acccctcgtc gatgaatatc gtctcgagac ggattccccc ggcgtatgac tgaacgacgt
     3241 ccgcgagacc catggccagc gacagcgatg ccagacagct ctcgccgccg gagagcgtct
     3301 gcaccggccg ggtcgttccc gtgtaagcgt cgtacacgag cagatccagt ccgccggcga
     3361 tccgaagatc cacccgttcc gtcgcgcgct ggaggtcgaa ccggccccgg ctcatgagac
     3421 gcaggcggtg ggaggccgcg gcgagcacat cctccagcag cgccgcgagc acgtagcgct
     3481 gcagcgtgac gccataaata ttcttcccgt tggccacctc cgccacccgt cccaccaccg
     3541 ccagctgcac ctccatttgc gcctgttccc cttgcagaaa cttcagctcc ccgaccgaac
     3601 tatcgatctg ccggctttgc tcgcccgtgc gcgcccgttc ttcgagcagg ccgttcacct
     3661 cggcctgggc attcgccgcg tcggtctcga gcgcggccaa gtcggggcgg actttcccgc
     3721 gcgcggcgtc gacgacgcga tcgagacgcg cctgggcggc cacctgcgcg tcacggaagg
     3781 tccggatctc ctgttcgagc tggtcgcgtt cctcctgctc gagcctggct ccctggcaag
     3841 cctcctcgtc gacaaacccg gcctccccga gacgcttcag aaaatcctcc acgaccgctt
     3901 ccaggcgttc gcgagtttcg ttgacctcgc gctccatgcc atcgagctgt gcctggcagg
     3961 cctgccacgc cgccacggcc gtgtcgtggt tttgacgggc atgctcgagc gcggttttca
     4021 gcgcctcatg ccgcgcctgt gacgccgcca gcgccgcgcc cagggcctcg agagagcgca
     4081 gcgtcgcggg aatgtccttt tcgagaccgg caatgcgttc ctgcaagcgg ccgtgtgcgg
     4141 tgctcacctc ggtgaagatc tcctccagcc ggggcaactc ctcggtaagc ctcgtttcgg
     4201 ttgcctgcag ctcggcgatt gtccggcgca agtcaccctg ctcctccgag gcagccttgg
     4261 ccttatcgag ctcggcctgc gcctcctcga gttgtttcgc cagtgccgcg ggagaagtct
     4321 tgcgatgtac tccgagatcc tccaggcact tggcgatgcg ttccttcgtg atggcgacac
     4381 gttgcacaac ctggccggct tcctgcacgg cggcgtcctg cgtcttttcg agacgcggca
     4441 actcctcttg caatgccgcg atttcctgtt catccggcac cgcggagcga aaatgcgccg
     4501 gtgccgggtg gcgcgtggag ccacataccg gacagggttc cccgcgcttg agctgtttcg
     4561 ccagcaccgt ggactgcgcc ttcactctct gctcttgcag gcgttcgaat cttttcttgg
     4621 cctcgttgag ggatttcttc gtttccctca tcagcgcctc ggcactcgcg agcgcgtgtt
     4681 cctgcatctt gagccggccc tgatgttccg ccagatcggc ggcgatcttg tgcagccgtt
     4741 gcagttcctt gacctgcgcc ctccactcgg cggttttacc ggacaactcg atcattctca
     4801 cgagatcctt ttggtgcttg cgcagcgcgc gggtgcactc gcgcagttcc tcttgcgccc
     4861 ggttccggtc gtgagtggcc tgcgtgacgc gccgcgaatg ttcggcgagt tgcgtgttcc
     4921 cctgttcgag ttccccgatc ttgccggtca gggcttcgag gcgcgctatc tctatccgga
     4981 caagctcgcg ttccgcttca cgggcctgtt cggccgccaa cgcgcgctcc gcggcatgct
     5041 ttgcctccag cgcccgttca tgttgcgtga cgaagttcgt ccttttgaga aggaccgctt
     5101 cgtgttcctg cttgcgctgc tggcggtaaa cctcagcgtc gcgcaaggcc tcggcgcgtc
     5161 gggcctgttc gaggcgctgg tcgagcgcat cgatttccgc gcgtcgcgcc tccgtccggg
     5221 catacctggc ccgggcctcc gtcaactcca cgaacacctt gtcaatctga tgcgcgtcgc
     5281 gcaattgctt gtcgaccagc tcccgtcgcg ccgcggcggc ggtcaatccg gcctcgacct
     5341 cgccggcgcg ccggcgcagt gcctcctgcc ggtcgaggag ttcctcggcg gtcgccactc
     5401 cagcctgtgc caggacaaaa ccgctccgtc cggtgagatc ctcgcaacgc cggcgaacgt
     5461 cgaccgccga ttgcttgagt gcctcctcga tttggcggta gaactcggtc gagaacagta
     5521 cttcgagaat ccgctggcgc tccgttgaat tcgccatcag gaaccgctgg aactggtact
     5581 gcggcaacat cacgacttgg cgaaactgat cgcaacgaaa accgagcagt tgctcgacct
     5641 tctcggtgac gtgtatccat tgggtcgcca gcacggtccc gtcgtccgcg tcatcggcca
     5701 tatcgctgcg gcgccagagc gtcgcttcgg gcggctcgcg cgtcacaccc gtgccttttt
     5761 tcttgggacg ctcctgctcc ggcatccgtt tgatacggta gcgctcggcg cccacggaaa
     5821 aatcgaagct gacttccgtc ggcacgtcgg ggggcgcatg atcgctgcgc agggtacggg
     5881 gctcacgcgc cgctcccgag gactctccgt aaagcgcgaa gcagatggca tccaggatcg
     5941 aggtcttgcc agcgccggtc tggccgtgga tcaggaaaag cgagcgtcca cccagctggc
     6001 ggaaatcgat ctcctgcctg gcggcatacg gcccgaaggc ctgcatcacg aggcgcaggg
     6061 gtttcacgac tgggattccc gttccaaagc gtgcagccgt tcgacgatgc ccaggtaggc
     6121 attgatctcg tcctcggacg cggcttcgcc cttcaccgtc tggaagaagg aagtaaaaat
     6181 ctcgcgatcg ctcaggttgc gataatccac gcgcgacgcc gccggatcac cccggccgaa
     6241 tcgctccatc cgcttgagct ccagcacgtt gggatacttg gaccgcaacc ggccgatggg
     6301 atcgaatacc ggacccttgt cgagaagcgt caccgacagg tagtcgtcgg catcgtttcc
     6361 gaccggcgcg tttaacaacg cttccagcgt cccagtgaca cagcgcacgg cgcggcgcgg
     6421 tgcgagcggg atccgctcga ggcggcatcg gcccgcggca tcgagttcca ccaggttgac
     6481 cgacttggaa tgcgtggcct cggagaagga gtattgcaac agcgatcccg agtagtggat
     6541 ccggtcgttg ccggcccgtt gcgggcgatg cagatggccg agcgcgacga aggaaaaacc
     6601 gtcgaacacc gacggttcga cggcgcccgt ggcgcccact gacagcggac gctcggactc
     6661 ggattcctcg ctcccggcga cgaaggcatg ggccacgagc accgagcgcg tgccgcgcgg
     6721 gcgagtctcg cgtgctttcg cgatgacagc gcgcatcgcg gcggcatgat cgggaaggtc
     6781 cgggttgccg agacgctcgc gaaccagggc gggctcggca taaggaatcg cgcagacgct
     6841 cacggtgccg tggttgtccg tgagcgacgt ccacatcggg tcgaggccga cacgccctgc
     6901 cacgtgcaaa ccgctctggg agagcagccg cgtgccaaaa ccaaggcgct caggactgtc
     6961 gtggttgccg gcgatcagta tgacccgcgt cttgaggtcg atgacaagtc gcgagagggt
     7021 ctcatcgagc aggctcactg cctcaggtgg tggaacggcg cggtcgtaca cgtctcccgc
     7081 gatgacaacg gcatccggtt ttgcctcacg gaccagcttt atgaactggt ccagtacgta
     7141 ggcttggtcc tgcgtcaggt ggataccgta aaagatccga ccgagatgcc aatcggcggt
     7201 atgaattatt cgcatcctgc caaccccgag tccttgcgcg acgattccgt agtgtccgaa
     7261 attccgcacg ggacttcaat gtgccccagc ttaccaacgg ataacgtgcg ctgacgaatg
     7321 gcggaaaatt atcattcgta actattacat cgggcgtttg aattcaattc ctcatgggac
     7381 aaataaaaat ggggctaaag gccccatttt tatttgactg gcggagaggg gtgaggcggg
     7441 gtttcc
//
LOCUS       DBNGZE010000320         3050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1434731, whole genome shotgun sequence.
ACCESSION   DBNGZE010000320 DBNGZE010000000
VERSION     DBNGZE010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1434731"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..826
                     /locus_tag="AC16FV_08320"
     CDS             146..826
                     /locus_tag="AC16FV_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HIR2850095.1"
                     /translation="MASLISYYWNYIIDGLIFLFCAVMLGGYQVRLIRRSRQSPTRTV
                     QGMNALARTAWVEQAMEKGWDVVSVQTLRNATMAATLMASTAVILILGILNMLANADK
                     IGPTLHILNLTGTHDSSIWIFKLMLILVDFFIAFFAFSLAVRGYNHVGFLINVPVDKN
                     TKSHGVTPATVAVHLNRAASYYTIGMRTYYFSVPLLLWVFGSIWMIISTFGVLLVLKH
                     LDHLPQQD"
     gene            complement(827..1711)
                     /locus_tag="AC16FV_08325"
     CDS             complement(827..1711)
                     /locus_tag="AC16FV_08325"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850096.1"
                     /translation="MSPRSRFFIFIPSLVLLIGASPAPTETPAEIAAITNSAERGNQG
                     AQLLLGLSYREGRGLPRDARKAFYWIGKSARERNPYAQYVLGQMYAAGEGTARDTKQA
                     LDWWQQSAAQNQAQAQLALGEAYLYGKGTKQDYREAEHWLAKAADQGVAQAQYLLGRM
                     YQQGHGVPQDLERGRDWLSRAAAQGHSDAIKLLHFFADVGEDTMGIYEASREQLKKRA
                     DQGDVEAQYQLAIRYETGAWGVRRHPEQALYWFNQAAEKGYKPAMRSLADIYEKGSLG
                     VKPDAKLAAQWRERANQK"
     gene            complement(1743..2954)
                     /locus_tag="AC16FV_08330"
     CDS             complement(1743..2954)
                     /locus_tag="AC16FV_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2850097.1"
                     /translation="MNPVAPGHNPKKLPRTVVIVGLVSLLNDFASEMVVPLIPLLLAT
                     VLAAGPVALGLIEGVADTVSNLLKLWAGRHSDLYGRRRKPYVVFGYLLSNLVRPLIGF
                     SGSWLAVLSIRVTDRIGKGVRTAPRDALISDAIDDDVAGRAYGFTRALDHTGAVLGAL
                     VAAAVVYWGTARLDVVIALSAIPGVLAVLLFAFGIRETPRTAALSAERAPLSWSRLNP
                     VSRRYLMVLAFFSLGKIPETFLLLRGHELGMSVVELLLLWAVMHVVKAAIAEQAGSHT
                     DRVGRRPLILTGWMVYTITLFALAFVAQPLMLWAWSLALGFYFGLTEGAERALVRDLA
                     TPAERGTAFGWFHMLVGTAAIPAGLLLGGLWSFHGVKIAFLVSSGLACLATIGLWRWV
                     QIKPQNSAPGI"
BASE COUNT          601 a          940 c          933 g          576 t
ORIGIN      
        1 tccttgtggg gcggcaagca taggggaatt tccagcaaat ggaaaccgcg gattccttct
       61 gcgcagccaa tggcaaaggc tgcgcgacgg ggacaatttt gctaagatgc cgcccgcttc
      121 gattccaaaa acaatcccgg acccgatggc ctcgctgatt tcttattact ggaattacat
      181 tatcgacggc ctgattttcc tgttctgcgc cgtcatgctt ggcggttatc aagtacgttt
      241 gattcggcgc agtcgccaga gccccacgcg cacggtgcag ggcatgaacg cactggcgcg
      301 taccgcctgg gttgaacagg ccatggagaa gggttgggac gtggtatcgg tgcagacgtt
      361 gcgcaatgcc accatggccg ccacgctcat ggcctcgacg gcggtcatcc tgatcctcgg
      421 catcctgaac atgctggcga acgccgacaa gattggcccg acgctgcata ttctgaacct
      481 gaccggcacc cacgattcca gcatctggat cttcaagctg atgctgatcc tggtcgactt
      541 cttcatcgcc ttcttcgcgt tctcgctggc agtgcgcggg tacaaccatg tcggttttct
      601 gatcaacgtg ccggtggaca agaacacgaa gagccacggc gtcacgccgg ctacggtcgc
      661 ggtgcatttg aaccgcgcgg cgtcctacta caccatcggc atgcgcacct attatttttc
      721 cgtaccgctg ctgctgtggg tgttcggttc gatctggatg atcatctcga ccttcggcgt
      781 gctgctggtg cttaagcacc tcgatcatct gccgcagcag gactgattat ttctgattgg
      841 cgcgttcgcg ccactgcgcg gcgagcttcg catctggttt cacaccgagc gatcctttct
      901 cgtagatatc cgcgaggctg cgcatcgccg gcttgtagcc tttctccgcc gcctggttga
      961 accagtacaa cgcctgctcg ggatgccggc gcacgcccca agccccggtt tcatagcgaa
     1021 tggcgagctg gtattgcgcc tccacgtcac cctgatcggc gcgttttttc aattgctcgc
     1081 gcgaggcttc gtaaataccc atggtgtctt cgccgacgtc ggcgaagaag tgcagcagct
     1141 tgatcgcgtc ggaatgtccc tgggccgcgg cgcgtgacag ccagtcccgg ccgcgttcga
     1201 ggtcctgcgg cacgccatga ccctgctgat acattctgcc gagcagatat tgcgcctgcg
     1261 ccacgccctg atcggcggct ttggccagcc agtgctcggc ctctcggtaa tcctgtttgg
     1321 tgcctttccc gtaaagataa gcctcaccca gcgccaactg ggcctgtgcc tgattctgcg
     1381 ccgctgattg ctgccaccag tccaacgcct gtttcgtgtc gcgcgcggtg ccttcacccg
     1441 cggcgtacat ctgcccgagc acgtattggg cgtacggatt gcgttcacgc gccgatttgc
     1501 cgatccagta aaacgccttg cgcgcgtcgc gcggcaggcc gcgaccttca cggtaactga
     1561 ggccgagcag cagctgtgcg ccctgattgc cacgttccgc ggaattggtg atcgcggcga
     1621 tctccgcggg tgtttccgtg ggcgccggtg aggcgccaat taacagaacg agtgacggaa
     1681 taaatatgaa aaaacgggag cgtggtgaca tgggttcctt gtgcgattta tccaaataag
     1741 tctcagattc cgggggcgct attttgcggc ttgatctgca cccatcgcca gaggccgatc
     1801 gtagcgaggc aagccaggcc gctggaaacg agaaacgcga ttttgacgcc atggaaggac
     1861 cagagcccgc ccagcaagag accggcggga atggccgcgg ttcccaccag catgtgaaac
     1921 cagccgaagg cggtgccgcg ctcggctggt gtggcgaggt cacgcaccag cgcacgctcg
     1981 gcgccctcgg tcaggccaaa atagaaaccg agtgcgagac tccaggccca cagcatcagc
     2041 ggctgggcca cgaaggcgag cgcgaacagc gtgatggtat aaaccatcca gccggtcaga
     2101 atcagtggcc ggcgaccgac acggtcggtg tggctgcccg cctgttcggc gatggcggcc
     2161 ttgacgacat gcatcaccgc ccacagcagc agcagttcaa ccaccgacat gccgagttca
     2221 tgtccgcgca gcagcaggaa agtttcagga attttcccta gcgaaaaaaa cgccagcacc
     2281 atgagataac gccgcgagac cgggttcagg cgtgaccagc tcagcggcgc gcgttcggcg
     2341 gacagggctg cggtgcgcgg cgtttcccgg atgccgaagg cgaatagcag cactgccagc
     2401 acgccgggaa tggcggacag tgcaatcacg acatcgaggc gcgcagtacc ccaataaacc
     2461 acggcggccg ccacgagcgc cccgagtacc gcgccggtgt ggtcgagcgc acgcgtgaat
     2521 ccgtaggcac ggcccgcaac atcatcgtcg atcgcgtccg agatcagcgc gtcgcgcggc
     2581 gcggtgcgca cgcccttgcc gatacgatcg gtgacgcgga tcgagagcac ggcgagccag
     2641 ctgccggaaa atccaatcaa cggtcgcacc agattcgaca gcagatagcc gaacacgaca
     2701 tagggtttgc ggcgtcgtcc atagagatcg gaatgccgtc cggcccagag cttgagcaga
     2761 ttggagacag tgtcggctac gccctcgatc aatcccagcg ccaccgggcc ggcggcgagc
     2821 acggtggcca gcagcaacgg tatgagcggc accaccattt cgctggcaaa gtcgttgagc
     2881 aggctgacca ggccaacaat aacgacggtg cgcggaagtt ttttcggatt gtggccgggc
     2941 gcgaccggat tcaagggagg cgttcgcaca tgtcggggcg cttactttag cggaccctga
     3001 ctcggcaggc aaaaagcaaa accccagcac aggggtgagc cgcgaagcgg
//
LOCUS       DBNGZE010000321         2320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_45469, whole genome shotgun sequence.
ACCESSION   DBNGZE010000321 DBNGZE010000000
VERSION     DBNGZE010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_45469"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="AC16FV_08335"
     CDS             <1..373
                     /locus_tag="AC16FV_08335"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR2850098.1"
                     /translation="YLPVLSRLSHDRLWFAEAGPKTKLVSNRFGIPEPVVKASELVRA
                     QELDLILMPLVGFDDKGNRLGMGGGFYDRSLEFLRHRNHWRKPHLLGIAYDFQRVNGL
                     APDPWDIPLAGVITDQAVYLY"
     gene            401..1213
                     /locus_tag="AC16FV_08340"
     CDS             401..1213
                     /locus_tag="AC16FV_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR2850099.1"
                     /translation="MNLLFIGDVVGQPGRRAISDQLPTLRNRLKLDLVVANAENIADG
                     AGITKKTATELFAAGVDVLSNGNHAWDKKEALEYITTEPRLLRPHNYPDGTPGTGWHV
                     ATTSAGHKVGILNLMGNVFMHPHLACPFAAADKALAAKPDDIKIVLVDLHAEVTSEKM
                     AMGWYLDGRVSAVVGTHTHVPTADERIMPQGTGYITDVGMTGCYDSVIGMDTQKSLKR
                     FVHKLPERFEVAEGIGTVCGVLLDLDESTGLCRNITRLRVSESVAQTKVKVA"
     gene            1340..1810
                     /locus_tag="AC16FV_08345"
     CDS             1340..1810
                     /locus_tag="AC16FV_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR2850100.1"
                     /translation="MNYWLMKSEPAAFSIADLKSRPKKTEHWDGVRNYQARNFMRAMK
                     KGDLAFFYHSSCEVPGVYGIIEIARAAYPDHTAWDPENHHYDPKSTPERPLWFMVDVR
                     FKRELKKPVTLTAIKMQPALKQMRLVQRGSRLSVMPVTVKEWNTILKLAEEKSR"
     gene            1807..>2320
                     /locus_tag="AC16FV_08350"
     CDS             1807..>2320
                     /locus_tag="AC16FV_08350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2850101.1"
                     /translation="MKRLFALFAIFLFTAGLPVAAKGPADNPRVRMTTSLGVVELELD
                     AKRAPGTVRNFMNYVERGYYNGMIFHRVIPGFMIQGGGFVPGMREKTTGVPIKNEADN
                     GLKNLAGTIAMARTSDPQSAAAQFFINTVDNPALDHRDKTDRGWGYAVFGKVTKGMDV
                     AKKIENVATHT"
BASE COUNT          514 a          726 c          669 g          411 t
ORIGIN      
        1 ttacctgccg gtgctgtcgc gcctgtcgca cgaccgcctg tggttcgccg aggccgggcc
       61 gaagaccaag ctcgtctcca accgcttcgg cattcccgaa ccggtggtca aggcgagcga
      121 gctggtgcgc gcgcaggaac tcgacctgat cctcatgccc ctggtcggct tcgacgataa
      181 gggcaaccgg ctgggcatgg gcggtggttt ttacgaccgc agcctggaat tcctgcgcca
      241 ccggaaccat tggcgcaagc cacatttgct cggaatcgcg tatgatttcc agcgcgtcaa
      301 cgggctggcg cccgatccct gggacatccc actcgccggc gtcatcacgg accaggcagt
      361 ctatctttac taacagacat tgatttaaaa ggtaagcgtt atgaatctgc tctttatcgg
      421 cgacgtggtc ggtcaacccg gacgtcgcgc catttcagat caactgccca cgctgcgcaa
      481 ccgcctcaag ctcgatctgg tcgtggccaa cgccgagaac atcgccgacg gcgccggcat
      541 caccaaaaaa accgccaccg aactgttcgc cgccggcgtg gatgtactct ccaacggcaa
      601 ccacgcttgg gacaagaaag aagcgctcga atacatcacc accgaaccgc gcctgctgcg
      661 tccgcacaat tatcccgacg gcacgcctgg caccggctgg cacgtggcca cgaccagcgc
      721 gggtcacaag gtcggcatcc tgaacctcat gggcaacgtc ttcatgcacc cgcacctggc
      781 ctgcccgttc gccgccgccg acaaggcgct cgccgcgaaa ccggatgaca tcaagatcgt
      841 gctggtggac ctgcacgcgg aagtgacgag cgagaaaatg gccatgggct ggtatctcga
      901 cgggcgcgtg agcgcggtgg tgggcacgca cacgcacgtg ccgaccgccg atgagcgcat
      961 catgccccag ggcaccggct acatcaccga cgtgggcatg accggctgct acgattccgt
     1021 catcggcatg gacacgcaga aatcgctcaa gcgtttcgtg cacaaattgc cggagcgctt
     1081 cgaggtggcg gaaggcatcg gcaccgtttg cggcgtgctg ctcgatctcg acgaaagcac
     1141 cggcctgtgc cgcaacatca cgcgcctgcg ggtatccgag tccgtcgccc agaccaaggt
     1201 caaggtcgcc tagagaaaag gcatccgcag atgaacacag atgtaaatac agataaacgc
     1261 agattggata cacatgtcca tgtttttatc tgcgttcatc ttaaaataaa ttcgcgttaa
     1321 tctgcggttt tctgtagtta tgaattactg gctgatgaaa tccgagccgg ctgcattcag
     1381 catcgcggac ctgaaatcgc gtccgaaaaa aaccgaacac tgggacggcg tgcgtaatta
     1441 ccaggcgcgc aatttcatgc gcgccatgaa aaaaggcgat ctcgcgtttt tctatcattc
     1501 gagctgcgag gtaccgggcg tctacggcat catcgaaatc gcgcgcgcgg cctatcccga
     1561 ccacaccgcc tgggacccgg aaaaccatca ctacgatccg aagagcactc cggagcggcc
     1621 cttgtggttc atggtggacg tgcgcttcaa gcgtgaactc aaaaaacccg tgacgctcac
     1681 cgcgatcaaa atgcagccgg cactgaaaca gatgcggctg gtgcagcgcg gcagccgcct
     1741 gtcggtgatg ccggtcacgg tcaaggaatg gaacacgatc ctgaaacttg ccgaggagaa
     1801 atcccgatga aaagactgtt tgccctgttt gcgatcttcc tgtttaccgc cggcctgccc
     1861 gtcgcagcca aggggccggc ggacaatccg cgcgtgcgta tgaccaccag cctgggcgtc
     1921 gtcgaactcg agctggatgc caaacgcgcg ccagggacgg tgaggaattt catgaattat
     1981 gtcgagcgcg gctattacaa cggcatgata ttccaccgcg tgatcccggg gttcatgatc
     2041 cagggcggtg gcttcgtgcc cggcatgcgc gaaaagacca ctggcgtgcc gatcaagaac
     2101 gaggccgaca acgggctgaa aaacctcgcc ggcactatcg ccatggcgcg cacctccgac
     2161 ccgcaatccg cggcggcgca gttcttcatc aacaccgtgg acaatcccgc gctcgatcac
     2221 cgcgacaaaa ccgaccgcgg ctggggctac gccgtcttcg gcaaggtcac gaagggaatg
     2281 gacgtggcga agaaaatcga aaatgtcgcg acccacaccg
//
LOCUS       DBNGZE010000322         4093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_393282, whole genome shotgun sequence.
ACCESSION   DBNGZE010000322 DBNGZE010000000
VERSION     DBNGZE010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_393282"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16FV_08355"
     CDS             complement(<1..485)
                     /locus_tag="AC16FV_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR2850102.1"
                     /translation="MLDQNQKIRWINKPILFIVALVPLAFLIYRALNNDLGANPIETI
                     NRYTGDWVLRFLMITLAVTPLRRMTGWNGLLRYRRMLGLFAFFYVCLHFLSWAWLDQY
                     FVLADIVKDVVKRPYITVGFVSFLMLIPLAVTSTNAMIRRLGAKRWQRLHRLVYLIGI
                     GG"
     gene            complement(501..1463)
                     /gene="msrP"
                     /locus_tag="AC16FV_08360"
     CDS             complement(501..1463)
                     /gene="msrP"
                     /locus_tag="AC16FV_08360"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR2850103.1"
                     /translation="MRKPNDINPSEITPKDVYLNRRHFLREAAAVAAAGASAALLPSM
                     VLAGQKIHNMRKSPFSTREKPTSVQDITGYNNFYEFGTDKKDPARNAQNLRIFPWSVA
                     IEGEVKKPAVYQFEDLLKLFPLEERIYRLRCVEAWSMVVPWVGFPLRDLVRRVEPTSR
                     AKYVEFTTLHDPAQMPGQKPGLFGAGLDWPYVEGLRLDEAMHPLTILAVGLYGEMLPN
                     QNGAPIRLVVPWKYGFKSIKSIVKIRFVEKQPLTTWMKAGPSEYGFYSNVNPTVDHPR
                     WSQASERRIGEDSLFSPKRKTLMFNGYAEQVASLYTGMDLKKYF"
     gene            complement(1607..2491)
                     /gene="htpX"
                     /locus_tag="AC16FV_08365"
     CDS             complement(1607..2491)
                     /gene="htpX"
                     /locus_tag="AC16FV_08365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HIR2850104.1"
                     /translation="MKRILLFVATNIAVLLVLSITASILIRALGIEEMPSGLNLQSLI
                     VFAAVIGFGGSFISLAMSKWMAKRATGAMVIEQPRNATEQWLFETVRRQAKQAGIGMP
                     EVAVYDAPDINAFATGMKRDAALVAVSTGLLQSMDKDEAEAVLGHEITHVANGDMVTL
                     ALIQGVVNTFVIVLSRVVGYFVDRVLLKNERGYGIGFYVSTMVAQLVLGILASMIVMW
                     FSRQREFRADRGGAKLAGREKMIAALERLKAAHEPAQLPDQMAAFGISGGRDGGWKRL
                     FMSHPPLDERIAALQQSR"
     gene            2715..3956
                     /locus_tag="AC16FV_08370"
     CDS             2715..3956
                     /locus_tag="AC16FV_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850105.1"
                     /translation="MLAAPAMKLDDKPAEPQYREALVSMTALAFIEEHALLRPDQLED
                     AKKSLQALFLHTSGEETMLRKLIDILKTTKQIHQSFASISNVLSGISRGVQTVDGKVA
                     AYKQALATMTLSAEENAAFAGPFLSFSQALLQKMSSFDDNMHQYVELKETEARYDSIY
                     RIAMDARERLKQRLSGVLGSQTRSEVENRIRHNVVSSFDYSEAESNLQFARRDAQNKA
                     EEILEQLEDIRAMCQMAKNPSMREKGDDSTNISVTVSSYSATPAAKKTKPKVEYDDVF
                     ALFAKALRAHPRLLHLKDIVLELFKFYQHSYGMFRLDCNNLNKAIETMFYNSEAYFEA
                     KEEDKDIRTKREKLKMIEGLIPFLEHTAVAMNEDTALNYGRFSRKISDIISEKRAPWA
                     HIAEHLLRSKVQAEADLSTRL"
     gene            complement(4012..>4093)
                     /locus_tag="AC16FV_08375"
     CDS             complement(4012..>4093)
                     /locus_tag="AC16FV_08375"
                     /inference="COORDINATES: protein motif:HMM:NF049739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7577 domain-containing protein"
                     /protein_id="HIR2850106.1"
                     /translation="CSGCGEMNPAGFEICWQCGKPLDREP"
BASE COUNT          906 a         1237 c         1164 g          786 t
ORIGIN      
        1 ccaccgatgc cgatgagata caccaagcgg tgcagtcgtt gccagcgctt cgcgcccaga
       61 cgccggatca tggcattggt ggaagtcacg gcgagcggaa tcagcatgag aaagctcaca
      121 aaacccaccg tgatatacgg ccgcttgacc acgtccttga cgatgtccgc gagcacgaaa
      181 tactgatcga gccaggccca ggacaggaaa tgcaagcaga cgtagaaaaa ggcgaacaat
      241 cccagcatgc gccgataacg cagcaggccg ttccagccag tcatgcgccg cagcggtgtg
      301 acggccagcg tgatcatgag aaagcgcagc acccagtcac cggtgtagcg gttgatggtc
      361 tcgatgggat tggcgccaag atcgttgttc agggcacggt aaatcagaaa tgccaacggc
      421 accaatgcga caataaaaag tattggcttg ttgatccagc ggatcttctg attttgatcg
      481 agcatcgatg cctggcaaat ttaaaaatat ttcttcaaat ccatgccggt gtagagactc
      541 gcgacctgct cggcatagcc gttgaacatg agcgtcttgc gcttgggtga aaacaatgaa
      601 tcctcgccga tgcgccgctc actggcctgg ctccagcgcg ggtggtccac ggtcggattc
      661 acgttcgaat aaaaaccata ctcgctggga ccggccttca tccaggtggt cagcggctgt
      721 ttctccacga aacggatttt cacgatggac ttgatgctct tgaagccgta tttccacggt
      781 accacgaggc ggatcggcgc gccgttctgg ttcggcagca tttcaccgta caaccccacg
      841 gccaggatcg tgagcgggtg catggcctcg tcgaggcgca gaccttcgac atagggccag
      901 tcgagaccgg cgccgaacag gcccggtttc tggccgggca tctgcgccgg atcgtgcaac
      961 gtggtgaact cgacatactt cgcgcgcgag gtcggttcga cgcggcggac caggtcgcgc
     1021 aagggaaatc ccacccacgg caccaccatg gaccaggcct cgacgcagcg caggcgatag
     1081 atgcgttcct ccagcggaaa gagcttgagc aaatcctcga actgatacac cgcgggtttt
     1141 ttcacctcgc cctcgatggc gacactccag ggaaagatgc gaagattttg cgcgttgcgt
     1201 gccggatctt ttttgtccgt gccgaactcg tagaaattgt tgtagccggt gatgtcctga
     1261 acggaagtgg gtttttctct ggtggaaaac gggctcttgc gcatgttatg gattttctgc
     1321 ccagccagca ccatggacgg caacaacgcc gcgctggccc cggcagcggc cactgctgcg
     1381 gcctcgcgca ggaagtgccg gcgattcaga tagacatctt ttggtgtgat ttcactcggg
     1441 ttgatatcgt tgggttttcg aatgataacc atacctcttc tccgggggac tgttggcccc
     1501 atggtgtcgg tattgcgacc gtccttttga atattagttg ctgccggaaa atgaaaaacc
     1561 cgctgcgttt tcacgcagcg ggttcgggtt caccggcttg tcacgctcag cgcgactgtt
     1621 gcagggcggc gatgcgctcg tccagcggtg ggtgtgacat gaacaggcgc ttccagccgc
     1681 cgtcacgccc gccggagatg ccgaaggcag ccatctggtc gggcagctgc gccggttcgt
     1741 gcgccgcctt gagacgctcc aacgcggcga tcattttctc gcggccagcc agtttggcgc
     1801 cgccgcggtc ggcgcggaac tcgcgctggc ggctgaacca catgacgatc atgctcgcga
     1861 ggataccgag cacaagctgc gcaaccatgg tgctgacata aaagccgatg ccataaccac
     1921 gctcattctt gagcagcacg cgatcgacga aatagccaac gacgcgcgac agcacgatta
     1981 caaaggtgtt gaccacgccc tggatcagcg ccagcgtcac catgtcaccg ttggcgacgt
     2041 gcgtgatttc gtgacccagc accgcctcgg cctcgtcctt gtccatggac tgcaacaggc
     2101 cggtgctgac cgcgaccagc gccgcgtcgc gcttcatgcc ggtggcgaag gcgttgatgt
     2161 ccggggcgtc gtagaccgcc acctccggca tgccgatgcc agcctgcttg gcctggcgtc
     2221 gcaccgtctc gaacagccac tgctccgtgg cgttgcgcgg ctgttcgatg accatggcgc
     2281 cggtggcgcg cttggccatc cacttggaca tggcgagcga gatgaaagaa ccgccgaaac
     2341 cgatcaccgc tgcgaacacg atgagggact gcaggttcag tccgctcggc atctcctcga
     2401 tgccgagcgc gcgaatcagg atgctggcgg taatgctgag gaccagcagg acggcaatgt
     2461 tggtcgccac gaacagcaaa atgcgtttca taaaatttgg attctccagt gaagtaaacc
     2521 gttttcgagc ttgttagata gggtcagcgc gccgcaagtt caatccccga aatcgccggc
     2581 gccagccatc tgtttcatta ttacggacag ccggtaaccc cgtataagtg ctctataatt
     2641 tataatgaag cgcctggaca aagccagtcc accttttgca atcaccctgt ctggctgtgc
     2701 cattcaggaa cgccgtgctc gccgcaccag ccatgaagct tgacgacaaa ccggccgaac
     2761 cccaataccg cgaggcgctc gtctcgatga cggcgctcgc ctttatcgaa gagcacgcgc
     2821 tgttgcggcc tgaccaatta gaggacgcca aaaaaagcct gcaagcactg ttcctgcata
     2881 cgagcggcga ggaaaccatg ctgcgcaagc tcatcgacat cctcaaaacg accaagcaga
     2941 tacaccagtc gttcgccagc atctccaatg tcctctccgg catcagccgc ggcgtgcaga
     3001 cggtggatgg caaggtcgcc gcctacaaac aggccctggc caccatgacc ctgagcgccg
     3061 aggagaacgc cgccttcgcc ggaccattcc tgtcgttctc gcaggcactg ttgcagaaaa
     3121 tgtcgtcctt tgacgacaac atgcaccagt atgtcgaact caaggagacc gaggcgcgct
     3181 atgacagcat ctatcgcata gccatggacg cgcgcgaacg tctgaagcag cgtctttccg
     3241 gcgtgctcgg ttcgcagacg cgcagcgagg tcgaaaaccg catccggcac aacgtcgtca
     3301 gttccttcga ctacagcgag gcggaaagca atctccagtt cgcccggcgc gacgcgcaaa
     3361 acaaggcgga ggagatcctg gaacagctcg aggacatccg cgcgatgtgc cagatggcca
     3421 agaatccctc gatgcgcgag aaaggcgatg actccaccaa catctcggtg acagtttcat
     3481 cgtattccgc cacgccggcg gcgaagaaaa caaaacccaa ggtcgaatac gacgacgtgt
     3541 tcgccctgtt cgccaaggcc ctgcgcgcgc atccgcgcct gttgcacctc aaggatatcg
     3601 tgctggagct gttcaagttc taccagcatt cctacggcat gtttcgtctc gactgcaaca
     3661 acctcaacaa ggccatcgaa accatgttct ataactcgga ggcctacttc gaggccaaag
     3721 aggaggacaa ggatatccgc accaagcgcg aaaaactcaa gatgatcgag ggcctgattc
     3781 ccttcctgga gcataccgcc gtggccatga acgaagacac agcgctgaac tacggacgct
     3841 tctcgcgcaa gatttccgac atcatttcgg agaagcgtgc gccctgggcg catatcgccg
     3901 agcacctgct gcgctccaag gtacaggccg aggccgatct cagcacgcgt ctgtgagccg
     3961 tgatccgtaa atcgtgaatt gtgaaccgat ccgcgaatta cgactcacga tttacggttc
     4021 acggtccagc ggcttgccgc actgccagca gatttcaaac cccgccggat tcatctcgcc
     4081 gcaacctgag cac
//
LOCUS       DBNGZE010000323         6425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1713098, whole genome shotgun sequence.
ACCESSION   DBNGZE010000323 DBNGZE010000000
VERSION     DBNGZE010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1713098"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1637)
                     /gene="hemN"
                     /locus_tag="AC16FV_08380"
     CDS             complement(249..1637)
                     /gene="hemN"
                     /locus_tag="AC16FV_08380"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR2850107.1"
                     /translation="MNHQIIFDAELLRRYDKSGPRYTSYPTAVQFNADFTETDYRAQA
                     QRSNATPTPRPLSLYFHLPFCDTVCFYCACNKVVTKDRKRASPYLGRLHREIALQAEL
                     FDRSRTVEQLHWGGGTPTFISHEEMRELMGVTREHFRLRDDDKGEYGIEVDPREIRPE
                     TLAVLRGLGFNRLSMGVQDFEPAVQKAVNRIQSEAETFAVLKEARALGFRSINMDLIY
                     GLPHQTVESFGRTLEKIIAAGPDRLSVFNYAHLPEMFKPQRRIRAEDLPSPQQKLNIL
                     QLTIERLTQAGYVYIGMDHFARPDDELAQAQRNGTLYRNFQGYSTHAECDLVGMGITA
                     IGMVGDCYSQNRKTLEDYYAALDAGHLPVMRGVVLDADDKLRRAIITQLICHFTLDPD
                     AIERVHGIRFTDYFAAELADLRIMQQDGLLEMDAKSIRVSPAGKLLIRNICMVFDRYL
                     REKREQRYSKVI"
     gene            complement(1767..3257)
                     /gene="ubiD"
                     /locus_tag="AC16FV_08385"
     CDS             complement(1767..3257)
                     /gene="ubiD"
                     /locus_tag="AC16FV_08385"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HIR2850108.1"
                     /translation="MKFKNLREFIAHLEKLGELLRIPVPVDPRLEITEICQRTLRAQG
                     PALLFEKPKGSAIPLLGNLFGTTRRVALAMGRESPEELREVGRLLAFLKEPQLPRGIG
                     DAMEKLPEFKQLLHVVPRVVSDAPCRQHVIEGKDVDLSQLPIQTCWPDDAGPLITFGL
                     VITKGPYKERQNIGIYRQQVIGRNRVIMRWLPHRGGAVDFREWKEAHPGQRFPVAVAI
                     GADPATLLAAVTPMPDTLSEFQFAGLLRGARSEVVRCHNGALYAPASAEFVLEGYIEP
                     NEEELEGPFGDHTGYYNSREKFPVFTIERITHRDQPIYHSAYMGRSPHDEPSILAMAL
                     NEVFIPLLQKQFPEIVDFYLPPEGCSYRIACVSIKKRYAGHARRIMFGVWSYLRQFTY
                     TKFVIVTDDDINVREWKDVVWAMTTRMDPVRDTLLVENTPIDYLDFASPVSGLGGKMG
                     LDATNKWPGETARAWGRPISMSADVVRRVDELWRRLGIEEVMHPRP"
     gene            3364..3843
                     /gene="ubiT"
                     /locus_tag="AC16FV_08390"
     CDS             3364..3843
                     /gene="ubiT"
                     /locus_tag="AC16FV_08390"
                     /inference="COORDINATES: protein motif:HMM:NF047962.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis accessory
                     factor UbiT"
                     /protein_id="HIR2850109.1"
                     /translation="MPPHPLLLPLRFIPDAVHAELFARVFNHLLRGQPLAARLSEING
                     KSVCIDIRDAGAKLHFRVGQGRLRPAAPGHTDVRISGKVEDYWQLATRREDPDTLFFS
                     RRLCIEGDTETGVHVKNLLDALDYDWEAHFRDVLGASLGAAAYAVFQRTVGQYLTAR"
     gene            complement(3968..5521)
                     /locus_tag="AC16FV_08395"
     CDS             complement(3968..5521)
                     /locus_tag="AC16FV_08395"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR2850110.1"
                     /translation="MSTRTGIVIDTGLRSGPENIRLDRAQWRAAQETDTPECQVRFHY
                     YRPTVALGAFETVGHALRAEYCRDHGIDMVRRVSGGAAVYLDADQLCWTLTLSRPGVW
                     GERNLAEWLARLCEGVAHGLQRLGVDAVFAPPNDIEVAGRKLASGYIALSDSALLFQG
                     SLLLDHDTETMMKALRVPTEKLTPDGVRSARGRFATLREAGVAFAPAVLERSVLAAWA
                     ALLDVEFGDRLELTPEEITGDENHAPPAGNWDAEHDSWYQALVKTPGGVIYVRLRLSE
                     RGDTLEKIEFAGGVQVYPAYLFASLSSWLASTRLTWLDEHFDDFFRVYPHDLLGFTVE
                     DLRHGLYLALDRYAQQLQFELTPEQANTLMLHAPAPLAAADVARAASVMLVPYCAKPS
                     WCKWRHRDGCPDCGLCEVGEAYRLARERGMRVVTITNFEHLEKTLGELRAQDTRAYVG
                     MCCRHFYLKREYAFRAAGIPAVLMDITGSNCYELQQEDLAYAGKFEAQAHLDVEVLRK
                     VMRRVPLAR"
     gene            complement(5518..>6425)
                     /gene="ubiV"
                     /locus_tag="AC16FV_08400"
     CDS             complement(5518..>6425)
                     /gene="ubiV"
                     /locus_tag="AC16FV_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiV"
                     /protein_id="HIR2850111.1"
                     /translation="FPAMAMKLSLGPIPYFWPRERVFDFYARVAAAPVDIVYLGETVC
                     SKRRALRLPDWLDIAARLTAAGKEAVLSTLTLIEAESEVSYMRTIIENGRYPVEANDM
                     AAINMLADIAPFVIGPHINAYNTRALDLMARLGARRWVMPLELDRETLAALQSSRPAG
                     LETEIFVFGRLPLSFSARCFTARAHNLPKDDCGFRCADYPEGMPLQTREGQSFLNLNG
                     IQVQSGETYNLINEIGEMQVFGVDVLRLAPQPQGMFEIIDAFHRVVDGHLEPSAAADV
                     LAPYQTAGFCDGYWHGVPGMNRVHV"
BASE COUNT         1290 a         2017 c         1925 g         1193 t
ORIGIN      
        1 ggggaacgtc catcgcgcct atttacatca tgattaagga atatgttgca tcgaaattcc
       61 cgccgttcat catgcatgac cgccggaaat ttcccataca ttcaataagt aaattaaagt
      121 gcgtcgaata ccacatcgta cgcggttatc gacggcacgt gtaaacgatt gttaacgcag
      181 cggggtatcg ggggaaacaa acgctacact gatcggtttg cgacggacgg cggcgggggg
      241 accggccgtc agatcacctt ggaatagcgc tgttcgcgct tctcgcgcag atagcggtcg
      301 aacaccatgc agatattgcg gatcagcagc tttccggcag gcgatacacg aatcgacttc
      361 gcgtccatct cgagcaggcc gtcctgctgc atgatcctga ggtcggcaag ttccgcggcg
      421 aagtaatccg tgaaacggat cccgtgcacg cgctcgatgg cgtccggatc gagcgtgaaa
      481 tgacagatca gctgcgtgat gatcgcgcgg cgcagtttgt cgtcagcgtc gagcacgacc
      541 ccgcgcatca ccggcagatg gccggcgtcg agcgcggcat aataatcctc cagggttttc
      601 cggttctggc tgtagcaatc gcccaccatg ccgatggccg tgatgcccat gccgaccaaa
      661 tcgcattcgg catgcgtcga gtagccctgg aaattgcggt acagcgtgcc gttgcgctgc
      721 gcctgcgcca gctcgtcatc cggacgcgcg aaatgatcca tgccgatgta cacataaccg
      781 gcctgcgtca ggcgctcgat ggtgagttgc aggatattga gcttctgctg cgggctcggc
      841 aggtcttccg cgcgaatgcg ccgttgcggt ttgaacattt cgggcaagtg cgcgtaatta
      901 aacaccgaca gccgatcagg accggcggcg atgatctttt ccagggtgcg tccgaaactt
      961 tcgacggtct ggtgcggcag cccgtaaatc aggtccatgt tgatggagcg gaaaccgagc
     1021 gcgcgcgcct ccttgagcac cgcgaaggtc tcggcttcgc tttgaatgcg gttgaccgcc
     1081 ttctgcaccg cgggctcgaa atcctgcacg cccatgctca ggcgattgaa gccgagacca
     1141 cgcaacacgg ccagcgtttc cggtcgtatc tcgcgcgggt cgacctcgat gccgtattca
     1201 cccttatcgt cgtcgcgcaa acggaaatgc tcgcgcgtga cgcccatcag ctcgcgcatt
     1261 tcctcgtgac tgatgaaggt cggcgtgccg ccgccccaat gcaattgctc caccgtgcgc
     1321 gaacggtcga acagctccgc ttgcaacgcg atctcgcgat gcagccgtcc gagatagggc
     1381 gaggcacgct tgcggtcctt ggtgacgacc ttgttgcagg cgcagtagaa acagacggtg
     1441 tcgcagaaag ggagatggaa atacagcgac agcgggcgcg gcgtcggcgt tgcattgctg
     1501 cgttgcgcct gcgcccgata atccgtttcg gtgaaatccg cgttaaattg caccgcggtt
     1561 ggatacgacg tgtaacgcgg tccgcttttg tcatagcggc gcagcagttc ggcgtcaaaa
     1621 atgatctggt gattcaagcg cggttaccgc aaagtcggtg gaaatattcg tcaaacatag
     1681 cgccaggcgg gcggaaaacc atgacttgta tcaataataa acgctgaaat aaagcggaaa
     1741 cgggcggaaa tacggtttgg ccgggctcac ggacgcgggt gcataacttc ctcgatgccg
     1801 agccggcgcc acagctcgtc cacccggcgc accacgtccg cgctcatgga gatcggccgg
     1861 ccccaggcgc gcgcggtttc gcccggccat ttgttggtcg catcgagtcc catcttgcca
     1921 ccgagtcccg agaccgggct ggcgaaatcc agataatcga tgggggtgtt ctcgaccagc
     1981 agcgtgtcac gcacgggatc catacgcgtg gtcatggccc acacgacatc cttccactcg
     2041 cgcacgttaa tatcatcgtc cgtcacgatc acgaatttgg tgtaggtgaa ctggcgcagg
     2101 tacgaccaca cgccgaacat gatgcggcgt gcgtgacccg cataacgctt cttgatgctg
     2161 acacaggcga tgcggtagga acagccttcc ggcggcagat agaaatccac gatctcggga
     2221 aactgtttct gcaacagcgg aatgaacacc tcgttgagcg ccatcgccag aatcgatggc
     2281 tcgtcgtgcg gcgagcgccc catgtaggcg ctgtgataaa tcggctggtc gcgatgcgtg
     2341 atacgctcga tggtgaacac cggaaatttt tcccgcgagt tgtaataacc ggtgtgatcg
     2401 ccgaacggtc cttccagctc ctcctcgttt ggctcgatgt aaccttccag cacgaactcc
     2461 gccgaagccg gcgcatacag cgcgccgttg tgacagcgca ccacctcgct gcgcgctccg
     2521 cgtaataatc ccgcgaactg gaattccgac aaggtatccg gcatcggcgt caccgccgcc
     2581 agcaacgtcg ccgggtcggc gccaatcgcc accgccacgg gaaagcgctg gccgggatgg
     2641 gcctccttcc actcgcggaa atccaccgca ccgccgcggt gcggcagcca gcgcatgatc
     2701 acccggttgc ggccgatgac ctgttgacga tagataccga tgttctgcct ttccttgtac
     2761 gggcccttgg tgatcacgag gccaaaggta atgagcggac cggcatcgtc cggccagcag
     2821 gtctgaatgg gaagttgcga caggtccaca tccttgcctt cgatgacatg ctgccggcac
     2881 ggcgcgtcgc tcaccacccg cggcaccaca tgcagcaact gcttgaactc cggcagtttc
     2941 tccatggcat cgccgatgcc gcgcggcagt tgcggctcct tcaggaacgc aagcagcctg
     3001 ccgacctcgc gcagttcctc aggggactcg cgccccatgg cgagcgccac gcggcgcgtg
     3061 gtgccaaaaa gattgcccaa cagagggata gccgatccct ttggcttttc gaacagcagc
     3121 gccggtccct gcgcgcgcag ggtacgctgg catatttcgg taatctccag ccgcgggtcg
     3181 accgggaccg ggatacgcag caactcaccg agcttttcca gatgcgcgat gaactcgcgc
     3241 aggttcttga atttcatgaa gcccctacaa taggacgcgc gacaagcgcc tgacttgacc
     3301 cagatcaaca aggcgcggcg tcggcgcctg tataaagcct ccgttcacca tttctcgact
     3361 tatatgccac cccatccact gcttttgccg ctgcgcttca tccccgacgc cgtgcacgcg
     3421 gaattgttcg cgcgggtgtt caaccacctg ctgcgcggcc agccgctggc ggcgcgcctg
     3481 tctgaaatca acggcaaatc cgtttgtatt gatatccgcg acgcaggagc caaactccat
     3541 ttccgcgtcg ggcaggggcg cctgcggccg gccgcgccgg gacacaccga cgtgcgcatc
     3601 agcggcaagg tggaggatta ctggcagctc gccacccggc gcgaggaccc ggatacgctg
     3661 ttcttcagcc gccgactctg cattgaaggc gacaccgaaa ccggcgtgca cgtgaagaac
     3721 ctgctcgatg cgctcgacta cgactgggag gcgcatttcc gcgacgtcct cggcgccagt
     3781 ctcggtgccg cggcatatgc cgtctttcag cgcaccgtcg gccaatacct cacggcgcgt
     3841 taagaaaagc ttgaataata tctcttgccg caaagacgca aagagcgcaa aggtttttgt
     3901 gtattttatt tcatgagttt ttctttgcgt tctctgcgtc tctgcggcaa aaaattcata
     3961 taaattttta tcttgccagc ggcacgcgcc gcatcacctt gcgcagcact tccacatcga
     4021 gatgcgcctg cgcctcgaat ttccccgcat aggccaggtc ctcctgctgc agctcgtagc
     4081 agttggaacc ggtgatatcc atcagcacgg cggggatgcc ggccgcgcgg aaggcgtatt
     4141 cgcgcttgag atagaaatgc cggcagcaca tcccaacgta ggcgcgcgta tcctgcgcgc
     4201 gcagctcacc aagggtcttt tccagatgct cgaaattcgt gatcgtgacg acacgcatgc
     4261 cgcgttcgcg cgcgagacgg tatgcctcgc cgacctcgca caggccgcaa tcgggacaac
     4321 cgtcccggtg acgccacttg caccaggacg gcttggcgca atagggcacc agcatcacgc
     4381 tggccgcgcg cgccacgtcc gccgccgcca gcggcgccgg cgcgtgcaac atgagcgtgt
     4441 tggcctgttc gggggtcagc tcaaactgaa gctgctgggc atagcggtcg agcgccagat
     4501 acaggccatg ccgcaggtct tcaacggtaa agcccagcag gtcgtgcgga tagacgcgga
     4561 aaaaatcatc gaagtgctca tccagccatg tcagacgcgt actggcaagc caggatgaaa
     4621 gcgaggcaaa caaatacgcc ggatagacct gcacgccacc ggcaaactcg attttctcca
     4681 aggtgtcgcc acgctcactg aggcgcaggc ggacatatat aaccccgccc ggcgttttta
     4741 ccagggcctg ataccaggaa tcgtgttccg catcccaatt accggcaggc ggcgcgtgat
     4801 tctcatcgcc tgttatttcc tccggcgtca attccagcct atcgccaaac tccacgtcca
     4861 gcaacgcggc ccaggcggcg agcacagacc gttccaagac cgccggcgcg aaggcgacgc
     4921 ccgcctcccg cagcgtggca aaacgaccgc gcgcgctgcg gacgccgtcc ggtgtgagct
     4981 tttccgtcgg gacgcgcaac gccttcatca tggtttcggt gtcgtgatcg agcaggagac
     5041 tgccctgaaa caacagcgcc gaatccgaca gtgcgatgta acccgaggcc agcttgcgcc
     5101 ccgccacttc gatgtcgttg ggcggcgcga acacggcgtc cacacccagc cgttgcagac
     5161 cgtgcgccac gccttcgcac agccgcgcga gccattcagc aagatttctt tcgccccaga
     5221 cgcccggacg cgacagcgtc agcgtccagc acagctgatc ggcgtcgaga taaaccgccg
     5281 cgccgccgct cacacgccgc accatgtcga tgccatgatc gcggcagtat tccgcgcgca
     5341 gggcatggcc cacggtttca aaagcaccga gtgcgacggt gggacgatag taatggaagc
     5401 ggacttggca ttcgggcgta tccgtctcct gcgccgcgcg ccactgcgcg cggtcgagcc
     5461 ggatgttctc cgggccggaa cgcagcccgg tatcgatgac aatgccggtg cgcgtgctca
     5521 tacgtgcacc cggttcatgc ccggcacgcc gtgccaatag ccgtcgcaga aaccggccgt
     5581 ctgatacggc gcgagaacat cggctgccgc agatggttct agatggccgt ccacgacacg
     5641 atggaaggca tcgatgatct cgaacatgcc ttgcggctgg ggcgcgagcc gcaggacatc
     5701 caccccgaac acctgcattt ccccgatttc gttgatcagg ttgtaggtct cgccggactg
     5761 cacctgaatg ccgttgagat tgagaaagga ctggccctcg cgcgtttgca gcggcatgcc
     5821 ttcgggataa tcggcgcagc ggaaaccgca atcgtccttt ggcaggttat gcgcgcgggc
     5881 ggtgaagcag cgcgcggaaa atgacagcgg caggcggccg aacacaaata tctcggtctc
     5941 caggccggcc ggacggctgg actggagcgc cgccagcgtt tcgcgatcga gctccagcgg
     6001 catcacccag cgccgggcgc cgaggcgcgc catgagatcg agcgcgcggg tgttgtaggc
     6061 attgatatgc ggaccaatga cgaacggagc aatgtctgcc agcatgttga tcgccgccat
     6121 gtcgttggcc tcgaccgggt aacggccgtt ttcgatgatc gtgcgcatgt aggaaacttc
     6181 cgattcggcc tcgatcagcg tgagcgtgga cagcaccgcc tccttgcccg cggcggtcag
     6241 gcgcgcggcg atgtcgagcc agtccggcag gcgcagggcg cggcgcttgg agcagaccgt
     6301 ctcgcccagg taaacgatgt ccaccggtgc agccgcgacg cgcgcgtaga aatcgaacac
     6361 gcgctcgcgc ggccagaaat acggaatcgg accgagggaa agtttcattg ccatggccgg
     6421 gaata
//
LOCUS       DBNGZE010000324         5053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_533261, whole genome shotgun sequence.
ACCESSION   DBNGZE010000324 DBNGZE010000000
VERSION     DBNGZE010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_533261"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /gene="fabG"
                     /locus_tag="AC16FV_08405"
     CDS             <1..641
                     /gene="fabG"
                     /locus_tag="AC16FV_08405"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR2850112.1"
                     /translation="ATSADGAEKITQALKQNNVAGRGVVLDVNSTDSIAAALSELEKS
                     GATPTILVNNAGITRDNLLLRMTEDEWDAILNTNLKSVYRMTKVCLRAMTKARWGRII
                     SISSVVGAIGNAGQVNYAAAKAGLIGFTKALAREVGSRNITVNAVAPGFIDTDMTRAL
                     PEAQREALLKQIPLGRLGLAEEVAAAVAFLASPQAGYITGTTLHVNGGMYMN"
     gene            761..997
                     /gene="acpP"
                     /locus_tag="AC16FV_08410"
     CDS             761..997
                     /gene="acpP"
                     /locus_tag="AC16FV_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR2850113.1"
                     /translation="MSSVEERVKKIVVEQLGVNEGEVKPAASFVDDLGADSLDTVELV
                     MALEEEFDCEIPDEQAEKITTVQQAIDYVNSRLN"
     gene            1097..2341
                     /gene="fabF"
                     /locus_tag="AC16FV_08415"
     CDS             1097..2341
                     /gene="fabF"
                     /locus_tag="AC16FV_08415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR2850114.1"
                     /translation="MSKRRVVITGLGILSPIGIGVAENWRNILAGKGGIGPITLFDAT
                     GYASTIAGEVKNFNPAAFGISDKEARRMDRFIQLGMAAGIEAIKDSGIQVTEANAERI
                     GVHLGSGIGGVGTIEDTTLTIKEKGPRRVSPFYVPMSIINMISGDLSVMYGLKGPNLA
                     MVTACATATHGIGDAGRLIEYGDADVMIAGGGEAAITPTAIAGFGNAKALSTRNDSPE
                     TASRPWDKDRDGFVLGEGAGVVVLEEYEHAKKRGAKIYCELVGFGMSGDAYHMTSPPE
                     NGEGAARCMRNALRNAGLNPDQVQYVNAHGTSTPLGDKAETQAVRAAFGDYARKLAVS
                     STKSMTGHLLGAAGGIEAVYSALAVHHQVAPPTINLITPDPECDLDYVPNTARQMKID
                     VAISNSFGFGGTNGTLVFRKLA"
     gene            2338..3747
                     /locus_tag="AC16FV_08420"
     CDS             2338..3747
                     /locus_tag="AC16FV_08420"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIR2850115.1"
                     /translation="MNARPAQKTVAAPGYVCRRLTALPDLVALHSRFPERYPHLLASV
                     AHGTPRARYDILFAFPGATLTLEADSVLYRDGQALGPGNFLDTFDRDWRAQSGTGGES
                     IVPEDALPFTGGWFVFLGYELVSQIEPTVSGIHFDAAFPVACATRVPAAIIIDRARQQ
                     TWLMCESERSKDLMPMLEQDALTSSAATAETLVLKDIHEEEPQRFLDHVRVVQEYIRA
                     GDVFQVNLSRLWRLTLNAPVKSAQLYRRLCAANPAPFAGLMTWGDDRAVISSSPERLV
                     AVRGRTVHTRPIAGTYPRSPDAAQDRALSQDLLRHPKERAEHVMLIDLERNDLGRVCR
                     TGSVQVKELMMLESYRHVHHIVSEVTGELREDVTPAQVIRAVFPGGTITGCPKVRCMQ
                     IIAELEQAPRTAYTGSMGYINCDGSMDLNILIRTIIRNGPEISLRAGAGIVADSVPER
                     ELAETRAKAKGMLAALGVY"
     gene            3865..4683
                     /gene="pabC"
                     /locus_tag="AC16FV_08425"
     CDS             3865..4683
                     /gene="pabC"
                     /locus_tag="AC16FV_08425"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:NF004761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HIR2850116.1"
                     /translation="MKILINGESGVSVSAQDRGFQYGDGIFETLAVADGKPLLWDRHM
                     RRFFHGAARLGFKTPAEELLRREAGQVCDGVKRGVLKIILTRGISGRGYAPSMDATPT
                     RTVGLMPWLDYPAQHRTDGVSVQFCHNLITRHNVLAGLKHLNRLEQILARMELRSDSA
                     EGLMQDETGHVIEGTMTNAFIVSRGVLLTPDLTYSGVEGVMRGLVLERAAALSIDCRI
                     TELMREDILGAEEIFLTNCLIGVWPVRQIGSRNYPVGRITQQIQDAIRDAIVAD"
     gene            4664..>5053
                     /locus_tag="AC16FV_08430"
     CDS             4664..>5053
                     /locus_tag="AC16FV_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR2850117.1"
                     /translation="MRSLLIKLAAGAAVICVIGAGYLVWAWNHALYPGAEIYDVKPGM
                     SLRGFARELSARGVLPESHSFVWLAHLTGRARGLKSGEYRFRDGMTASELLDQVVAGR
                     VIEYPLVLLEGWTFQQFLTTIREAPKLT"
BASE COUNT          994 a         1489 c         1596 g          974 t
ORIGIN      
        1 ccgccacttc ggccgacggc gcggaaaaaa tcacacaggc cctgaagcaa aataatgtcg
       61 ccgggagagg ggtcgtgttg gacgtgaaca gcaccgattc catcgccgcg gccctgtcgg
      121 aactggagaa atccggcgcc acgccgacga tactggtcaa caacgccggc atcacgcgcg
      181 acaacctgct gctgcgcatg accgaggacg agtgggatgc gatactcaac accaatctca
      241 agtcggtgta tcgcatgacc aaggtctgcc tgcgcgccat gaccaaggcg cgctggggac
      301 gcatcatcag catcagttcg gtggtgggcg cgatcgggaa cgcgggacag gtgaattacg
      361 ctgccgccaa ggccggcctg atcgggttca ccaaggcgct cgcgcgcgag gtggggtcgc
      421 gcaacatcac ggtcaatgcc gtggcgccgg gcttcatcga caccgatatg acccgcgccc
      481 tgcccgaggc acagcgggag gcgctgctca agcagatccc cctggggcgt ttgggactgg
      541 ccgaagaagt ggccgcggcc gtggcatttc tggcctcgcc gcaggcgggc tatattaccg
      601 gcacgacctt gcatgtgaac ggcgggatgt acatgaacta aagtggatat ctgtctgaaa
      661 aaagccggtt atacgattgg ctggacggtt ttacgcaaat ggtgttaact atgcgcccgc
      721 acgagggcag taacgtcaat tcttggagga atgattaccc atgagcagtg ttgaagagcg
      781 cgtcaagaag attgtcgtgg agcagttggg cgttaacgag ggcgaggtca agcctgccgc
      841 ctccttcgtg gatgatttgg gcgcggattc cctggatacg gtcgagctgg taatggcgct
      901 ggaagaggaa tttgactgtg aaattcccga tgagcaggcg gaaaagatca ccaccgtcca
      961 gcaggccatc gactacgtca actcccgtct gaactgataa ctcattagaa taagaggccg
     1021 tcatcctcac cgggtggcgg ccttttggtt ttctaaagcg cggcggagcc gcgcccacat
     1081 gatgagggat tcggcattga gcaagcggcg cgtcgtcatt accggcctcg gtatcctgtc
     1141 tcctatcggc atcggtgtcg ccgaaaactg gcgcaatatc ctcgccggca agggcggcat
     1201 cggccccatc acgctgttcg atgccaccgg ttacgcatcc accatcgccg gtgaggtcaa
     1261 gaatttcaat cccgccgcgt tcggcatttc cgataaagaa gcgcgccgca tggaccgctt
     1321 tatccaattg ggcatggcgg ccgggatcga ggcgatcaag gactccggta tccaggtgac
     1381 cgaggcaaat gccgagcgca tcggcgtgca tctcggctcc ggcatcggcg gtgtcggcac
     1441 cattgaagac acgacgctca ccatcaaaga aaaaggcccg cgccgagtct cgccgtttta
     1501 tgttcccatg agcatcatca acatgatctc gggcgacctg tcggtgatgt acggcctcaa
     1561 gggcccgaat ctcgccatgg ttaccgcctg cgccaccgcc acccacggta tcggcgatgc
     1621 cggccgcctg attgaatatg gcgatgccga cgtcatgatc gccggcggtg gcgaggccgc
     1681 gatcacgccg accgcgatcg ccggattcgg taacgccaag gccctgtcca cgcgcaacga
     1741 cagcccggaa accgccagcc gtccgtggga caaggaccgt gacggttttg tgctcggcga
     1801 gggcgcgggc gtggtggtac tcgaagaata cgagcacgca aagaagcgtg gcgcgaaaat
     1861 ttattgcgag ctggtgggct tcggcatgag cggcgatgcc taccacatga ccagcccacc
     1921 ggagaacggc gagggcgcgg cgcgttgcat gcgtaatgcc ttgcgcaatg ccgggttgaa
     1981 tcccgatcag gtgcagtacg tcaacgccca cggcacttcc acgccgctcg gcgacaaggc
     2041 cgagacacag gcggtcaggg ccgccttcgg cgattacgcg cgcaagctcg cggtgagctc
     2101 gaccaagtcc atgaccggcc acctgctggg cgcggccggc ggcatcgagg cggtttattc
     2161 cgcgctcgcg gtgcatcacc aggtggcgcc cccgaccatc aacctcatca cacccgaccc
     2221 ggaatgcgac ctggattacg tgccgaacac ggcgcgccag atgaaaatcg acgtcgccat
     2281 ttccaactcc ttcggctttg gcggcaccaa cgggacgctg gtgttccgca aactggcgtg
     2341 aatgcccgtc cggcccagaa aacagttgcc gccccgggat atgtctgccg tcgcctgacg
     2401 gctcttcccg atctcgtcgc gcttcattcc cgttttcccg agcgttatcc gcatctgctg
     2461 gccagcgtgg cgcatggcac gccgcgcgcc cgctacgaca ttctgttcgc tttcccgggc
     2521 gcgactctga cgctggaggc ggattccgtg ctttatcgcg acgggcaggc gctcggcccc
     2581 ggtaattttc tggatacctt cgatcgcgat tggcgggcgc agtccggcac aggaggagag
     2641 agcattgtgc ctgaggatgc actgcctttt accggcggat ggttcgtgtt tctcggctat
     2701 gagctggtgt cccagatcga gccgacggtc tccggcatcc acttcgatgc ggcatttccg
     2761 gtagcctgcg ccacgcgcgt gccggcggcg atcatcattg accgcgcgcg gcagcagacc
     2821 tggctgatgt gtgagtccga acgatcgaaa gatctgatgc cgatgctgga gcaggacgcg
     2881 ctgacgtcct cggcagcaac cgccgaaacg ttagtcctga aggatatcca tgaggaggaa
     2941 ccgcaacgtt ttctcgatca tgtacgcgtg gtgcaggaat atatccgcgc cggcgatgtg
     3001 tttcaggtaa acctttcgcg cctgtggcgc ctgacactca acgcgccggt gaaatcggca
     3061 cagctctatc gtcggttatg cgcggccaat ccggcgccgt tcgccggcct gatgacctgg
     3121 ggcgatgacc gcgcggtaat cagctcgtcg ccggagcggc tggtggccgt gcgcgggcgt
     3181 acggtgcaca cgcgtcccat tgccggcact tatccgcgca gtcccgatgc cgcgcaggac
     3241 cgagccctgt cgcaggacct gttgcgtcat cccaaggagc gcgccgagca tgtgatgctc
     3301 atcgatctgg aacgcaacga cctcgggcgc gtgtgccgca ccggttcggt acaggtcaag
     3361 gagctcatga tgctcgagtc ctatcgtcat gtgcaccata tcgtttcgga agtgaccgga
     3421 gagttgcgcg aggatgtgac accggcacag gtaatccgcg ccgtgttccc cgggggcact
     3481 atcaccggct gccccaaggt gcgctgcatg cagatcatcg ccgagttgga gcaggcgccg
     3541 cgcacggcct ataccgggtc catgggctat atcaattgcg atggcagcat ggatttgaat
     3601 atcctgattc gcaccattat ccgcaatgga ccagaaatct ctctgcgtgc cggcgccggt
     3661 atcgtggcgg actccgttcc ggagcgggag ctggcggaga cccgcgccaa ggccaaaggc
     3721 atgctcgcgg cgttgggagt gtactgattt atatgattat ctgccgcaga ggacacaaag
     3781 gacgcaaaga aaaactcagg aggataaaaa actcgtgaaa tctttgcgat ctttgcatct
     3841 ttgcggcaag aaataatatt tcaaatgaaa atactgatta acggcgaatc gggcgtaagc
     3901 gtttcggcgc aggaccgcgg gtttcagtac ggcgacggaa tttttgaaac gttggccgtt
     3961 gccgacggaa aaccgctgtt atgggaccgg catatgcggc gtttttttca tggcgcggcg
     4021 cggctcggct tcaagacgcc tgccgaagaa ctgctgcgcc gcgaagccgg acaggtatgc
     4081 gacggcgtca agcgcggtgt attgaaaatt attctgacgc gcggcatttc cggacgcggt
     4141 tatgccccga gcatggacgc gacgccgaca cgtaccgttg gtctgatgcc atggctggat
     4201 tatcccgcgc agcatcgaac cgatggtgtg agcgtgcagt tctgtcataa cctcataaca
     4261 cgccataacg ttctggcggg gctcaagcat ctcaaccggc ttgagcagat tctggcgcgc
     4321 atggaattga ggagcgacag tgccgaaggt ctgatgcagg atgagaccgg ccatgtcatc
     4381 gaagggacca tgacgaatgc gtttatcgtg tcacgtggcg tgctgctgac gccggacctg
     4441 acgtacagtg gcgtggaagg ggtgatgcgt ggcctggtgc tggagcgtgc cgccgcgctt
     4501 tcaatagatt gccgcataac ggaactgatg cgggaggata ttctgggcgc cgaggaaatt
     4561 tttctgacca actgtctgat cggtgtgtgg ccggtgcgcc agattggatc ccggaattat
     4621 cccgtgggcc ggatcaccca gcagattcag gacgccattc gcgatgcgat cgttgctgat
     4681 taaactcgcg gccggcgcgg ccgtcatctg cgtcatcggc gccggctatc tggtttgggc
     4741 ctggaaccac gcgctctatc ccggcgcgga aatctatgac gtgaaaccgg gcatgagcct
     4801 gcgcggcttc gcgcgtgaac tcagcgcgcg cggggtgttg ccggagagcc attccttcgt
     4861 ctggctggcg cacctgaccg gtcgcgcccg tggcctcaaa tccggtgaat accgtttccg
     4921 cgacggcatg accgccagcg agctgctgga tcaggtcgtt gccggccggg tgatcgagta
     4981 tcccctggtg ctgctcgaag gctggacgtt ccagcagttt ctgacgacga tccgtgaggc
     5041 gcccaaactc acc
//
LOCUS       DBNGZE010000325         3591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_949371, whole genome shotgun sequence.
ACCESSION   DBNGZE010000325 DBNGZE010000000
VERSION     DBNGZE010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_949371"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1598)
                     /gene="der"
                     /locus_tag="AC16FV_08435"
     CDS             complement(213..1598)
                     /gene="der"
                     /locus_tag="AC16FV_08435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR2850118.1"
                     /translation="MKPVVVLVGRPNVGKSTLFNRLTRSRTALVADAPGLTRDRQYGD
                     GRVGDRPYFVVDTGGVVSGLTTAKDRGDVKSGVTAQVRAALIEADAVILIVDGREGVH
                     PADRQLVADLRRQGKPVWIAVNKAEGRDPATVVAEFYALGLGEPAAISAAHGEGVTEL
                     IQRVLEKLPVAEEIEQDETVPRIAVIGKPNVGKSTLVNALLGEQRVVVCDEPGTTRDS
                     IRVPLERDGRRYALIDTAGVRRRARIDDPLEKFSVLKTLQAIDEANVAILVIDAQAGV
                     AEQDASLAGYALEAGRALVVAVNKWDAVKESDRAWAKREIERKLPFLDFARVHFISAH
                     TGTGVGGLFRSVDEAYASAHRIMTTSKLNRILEAAVTATPPPLVHGRRARPKYAHQGG
                     KNPPRVVIHGNQVASLSKSYRRYLANAFRKAFHLIGTPVLIECRQEENPYQGKKTGGG
                     RGGRRTKRRRP"
     gene            complement(1731..2873)
                     /gene="bamB"
                     /locus_tag="AC16FV_08440"
                     /gene_synonym="yfgL"
     CDS             complement(1731..2873)
                     /gene="bamB"
                     /locus_tag="AC16FV_08440"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR2850119.1"
                     /translation="MKNAALILCLALLLPGCGGSKAVREEPAKLQDFAAEMRVRELWT
                     ADSGASAVKKAVVLSPALDGDVVFTSDPKGRVRAFAADNGRELWETHVDQLVTGATAA
                     GDGLVVVASKKGEVIALDRTDGRRLWSSSLSSEALAPAAIGAGVVVVQSVDGKLTGLS
                     ATDGKRLWRYERTEPPLSLYGTARPVIVADAVLTGFASGKVVAVQVRSGKLLWELPVA
                     QPQGRNEVERLVDVDVSPVVVRNVLYAASYQGKIIALDAQSGRILWSRDVSTFSGMDA
                     DANNIYLTDARGNVLAFDQRTGASVWKQEQLRARRLNAPRYVNGLIAVGDFEGYVHWL
                     SSDDGHFVARYRVGSSAVRSQALVGHDMLYVSNQSGDLAALRLEKN"
     gene            complement(2870..3508)
                     /locus_tag="AC16FV_08445"
     CDS             complement(2870..3508)
                     /locus_tag="AC16FV_08445"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR2850120.1"
                     /translation="MTAYDEQEELEKLKAWWKNYGSSAIFGVLLGGAILVGFRYWTQH
                     VEQQRYAAASLYERMFQDISAKKTGEAINLGESLINEYSSTPYAGMAGLMLARLDFEA
                     GDVAKARERLQWVLQHADDDAVRHAARLRLARIHLGNGDKEAALALLDVKDRAGFEAE
                     YEELKGDILVAQGQRDAARVAYGEALKHLPAGSPYAPMLNMKLDDLGPGKKS"
BASE COUNT          686 a         1191 c         1017 g          697 t
ORIGIN      
        1 agataagtga ttccgaagcc gccatggccc agaaccgatt caattgtgta ttccgccaac
       61 cggtaacccg ccgggagggc gctcttgtga aacgcgctca caccacggcc ccgtcgtgcc
      121 cctgaccctg aaactccatg ttttccaaca ccatatatat agaattttat ttatcagttc
      181 tgaatcactt ttcagtatag atcacacgga tttcacgggc ggcgacgctt tgtccggcgc
      241 ccacccctgc caccgccggt ttttttacct tgatacgggt tctcctcctg acggcactcg
      301 atcagcaccg gcgtgccgat caggtggaac gccttgcgaa aggcattggc gagatagcga
      361 cggtaggatt tggacagcga ggccacctgg ttaccgtgga tcaccacccg cggcggattc
      421 ttgccgccct gatgggcata cttgggccgg gcgcgccggc catgcaccag gggtgggggc
      481 gtggccgtta ccgccgcttc caggatgcgg ttcagcttgg acgttgtcat gatccggtgg
      541 gccgaggcat aggcctcatc caccgagcga aacagaccgc ccacgcccgt cccggtgtgc
      601 gccgaaataa aatgtacgcg ggcgaaatcg aggaacggca gtttgcgctc gatctcgcgc
      661 ttggcccacg cccggtcgga ttccttcacg gcgtcccatt tgttcacggc gaccaccagc
      721 gcccgcccgg cctccagcgc ataacccgcc agcgaggcat cctgctcggc cacgccggcc
      781 tgtgcgtcga tcacgaggat ggccacattg gcctcgtcga tcgcctgaag cgtcttgagc
      841 accgagaatt tttccagcgg atcgtcaatg cgcgcgcggc ggcgcacccc ggcggtgtcg
      901 atgagcgcat agcgtcgccc gtcgcgttcg agcggaacgc ggatactgtc gcgcgtggtg
      961 cccggttcat cgcacaccac cacgcgctgc tcgcccagta aggcattgac cagggtggac
     1021 ttgccgacat tgggcttgcc gatcacggcg atgcgcggga cggtttcatc ctgttcaatc
     1081 tcttccgcca ccggcagctt ttccagcacg cgctgaatca gttcggtcac gccctcccca
     1141 tgcgcggccg agatggccgc cggctcgccg agtcccaagg catagaactc cgcgacaacc
     1201 gtggccgggt cgcgcccttc cgccttgttc accgcgatcc acaccggctt gccctgtcgt
     1261 cgcagatccg cgaccaactg gcggtcggcg ggatgcacgc cctcacgccc gtccacgatg
     1321 aggatgacgg catcggcctc tatcagcgcg gcgcgcacct gggcggtaac gccagatttc
     1381 acgtcaccgc gatccttggc cgtggtcagg ccggacacca cgccgccggt atcgacgaca
     1441 aaatacggac gatcgccaac ccggccgtcg ccatactggc gatcgcgcgt cagccccggg
     1501 gcatcggcca ccagcgcggt gcgactgcgt gtcagccggt tgaagagcgt ggacttgccg
     1561 acattgggcc ggccgacgag aacaacgacg ggtttcatgt aaggaagttc taatttatat
     1621 gattatctgc cgcagagatg caaagatcgc aaagatttct cgggttttat tctcctgaga
     1681 ttttctttgc gtcctttgcg tctttgcggc aagaattcat aaatcagaaa ttaattcttc
     1741 tccaggcgca gcgcggcgag atcgccggat tgattgctga cataaagcat gtcatggccc
     1801 accagcgcct gggaccggac ggcgctgctg ccgacccgat aacgcgcgac aaagtggccg
     1861 tcgtcactcg acagccagtg gacatagccc tcgaaatccc cgacggcgat cagcccgttg
     1921 acgtagcgcg gcgcattcag ccggcgcgcg cgcagctgct cctgcttcca cacgctcgcg
     1981 ccggtgcgct ggtcaaacgc cagcacgtta ccgcgagcat cagtgagata aatgttgttt
     2041 gcgtccgcat ccatcccgga aaaagtggat acatcgcgcg accacaggat gcgtccgctt
     2101 tgcgcatcca gggcgatgat ttttccctga tagctggcgg cataaagcac gttgcgcacg
     2161 accaccggtg aaacgtcgac atccaccagg cgctccacct cgttgcgccc ttgcggttgc
     2221 gccaccggca attcccacag aagtttgccg ctgcgaacct gaaccgcgac caccttgcca
     2281 ctggcgaaac cagtcaatac cgcgtcagca acgattaccg gtctggccgt gccgtacaga
     2341 ctgagcggcg gttcggtgcg ctcgtaacgc cacaggcgct tgccgtccgt agccgacagc
     2401 ccggtaagct tgccatccac cgattgcaca acgacgacac cggcgccaat cgccgccggt
     2461 gcgagagcct cactcgacaa actgctggac cacagccggc ggccgtccgt ccgatcaagc
     2521 gcgatcacct cgcccttctt gctggcgacc accaccagac cgtcgccggc ggccgtggcg
     2581 cccgtcacca gctgatccac atgcgtttcc cataattcgc ggccattgtc agcagcgaag
     2641 gcacgcaccc gccccttggg gtcggaggtg aaaaccacgt cgccatccag cgcgggcgac
     2701 aacaccacgg cctttttgac cgcgctcgcg ccgctatccg ccgtccataa ctcgcgcacc
     2761 cgcatttcgg cggcaaagtc ctggagcttc gcgggctctt cgcgcacggc cttgctgccg
     2821 ccacaaccgg gcaggagcaa cgcaagacac aggatcaaag cggcattttt catgacttct
     2881 tccccggccc caggtcgtcg agcttcatgt taagcatcgg ggcgtagggt gaaccggcgg
     2941 gcagatgctt gagcgcctcg ccataagcca cgcgcgcggc atcgcgctgt ccttgcgcaa
     3001 ccagaatatc gcctttcaat tcttcgtatt cggcctcgaa acccgcacgg tccttcacgt
     3061 caagcagcgc gagcgccgcc tccttgtcgc cgttgcccag atgaatgcgc gccagacgca
     3121 gtcgcgcggc atgcctgacc gcgtcatcgt cggcatgttg cagcacccat tgcaggcgtt
     3181 cacgcgcttt cgcgacgtca ccggcttcga agtccagacg cgcaagcata aggccggcca
     3241 tgccggcata aggcgttgag gaatattcat tgatcaggga ttcgcccaga ttgatcgctt
     3301 caccggtttt tttcgcggaa atatcctgaa acatgcgctc ataaaggctg gcggcggcgt
     3361 accgttgctg ctccacatgc tgggtccagt agcggaaacc gacgaggata gccccgccca
     3421 gcaggacgcc aaagatcgcg gagctgccgt agttcttcca ccaggctttg agtttttcca
     3481 gttcttcctg ttcgtcgtaa gcggtcaaga tgatactcct tgtgaaattt ctttaagtac
     3541 taaatcaatt agttcgcctt gcgcgaacga gcgctgcgta ccattgcgta a
//
LOCUS       DBNGZE010000326         4427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1226992, whole genome shotgun sequence.
ACCESSION   DBNGZE010000326 DBNGZE010000000
VERSION     DBNGZE010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1226992"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="holA"
                     /locus_tag="AC16FV_08450"
     CDS             <1..459
                     /gene="holA"
                     /locus_tag="AC16FV_08450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR2850121.1"
                     /translation="RFSGGEVGLDDIEDNLSDNARYNVFALADASLRGEPATVERILT
                     NLRGEGVEPILVLWALAREARELAQMASLVASGQPVMRVLDARRVWAKRKPLVSAALK
                     RLSPETSQDLLRQAARTDRILKGRGTGDVWQELQCLALSMSGLKPATCHI"
     gene            489..1775
                     /locus_tag="AC16FV_08455"
     CDS             489..1775
                     /locus_tag="AC16FV_08455"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR2850122.1"
                     /translation="MKAASKQTTDADIKRYMQEVGQRARTAARMMARAETAAKDAALM
                     AMAAEIERQTDALIAANKKDIDAGKVQGLDSAMLDRLELNGARIKAMAEGLRQIAALA
                     DPVGEITDLNYRPSGIQVGRMRVPLGVIGIIYESRPNVTADAAGLCLKSGNASILRGG
                     SEAIHSNRAIAACIQIGLKQAGLPEDAVQVVETTDRAAVGELIRMKEFVDVIVPRGGK
                     SLIERISAEATIPIIKHLHGVCHVDIDDKADLDKAVKVAFNAKTQRYGTCNTMETLLV
                     ARGVAATVLPVLGKMYQDKGVELRGCEATRKILAGIKAATEEDWHTEYLAPILSIRVV
                     DNVDEAMDHIARYGSAHTDSIVTEDITRARRFLREVDSSSVMVNASTRFADGFEYGLG
                     AEIGISTDKLHARGPVGLEGLTSVKFIVLGDGHIRK"
     gene            1866..2513
                     /gene="nadD"
                     /locus_tag="AC16FV_08460"
     CDS             1866..2513
                     /gene="nadD"
                     /locus_tag="AC16FV_08460"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR2850123.1"
                     /translation="MGKPLGILGGTFDPIHYGHLRPAQEVLRALDLSEIRIIPAANPP
                     HRRAPLATAEQRLRMAELAVAEFPGLRVDDRELRRGGPSYTVLTLESLRRELGAQPLC
                     LLLGVDAFEGIETWHQWQRLPELAHFVVMTRPGWPPPAADVLPSWARGRLARVPDDLA
                     QAGAGKIYFQAVTPQDISATRIREAIARGEPVDGLLPPAVLEYLRANRIYLNQGN"
     gene            2513..2932
                     /gene="rsfS"
                     /locus_tag="AC16FV_08465"
     CDS             2513..2932
                     /gene="rsfS"
                     /locus_tag="AC16FV_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR2850124.1"
                     /translation="MPKATTTRKTQSWIQDALEDAKARDITVLDVRKISDFTDYMVIA
                     TGTSNRHVQSTSDKIVDALRSHGVRAVGVEGGKIGDWVLIDFGDVVVHVMREETRDFY
                     NLEKLWSDAKRVETGQKKKTAKAPRTPRKLKVKSGSG"
     gene            3031..3501
                     /gene="rlmH"
                     /locus_tag="AC16FV_08470"
     CDS             3031..3501
                     /gene="rlmH"
                     /locus_tag="AC16FV_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019441656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR2850125.1"
                     /translation="MQIHIIAIGDRMPEWVEAGYREYAKRLPRECRLVLHEIPAGRRT
                     KGADLRRLIEQEGARQMDAIPAGAHVVALDRTGKQLDTETLAAELKKRLGGGEHLALL
                     IGGPEGLAPPCLERAKERWALSKLTLAHPVVRVVLVEQLYRAWSIIQNLPYHRR"
     gene            3596..4174
                     /locus_tag="AC16FV_08475"
     CDS             3596..4174
                     /locus_tag="AC16FV_08475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR2850126.1"
                     /translation="MIYLASASPRRQELLRQLGIEFEVMPSNILEVREAGEAPAQYVT
                     RIARDKARWVAQLVRERELPAHPVLGADTEVVLDGEILGKPENAAHGMEMLRRLAGRT
                     HEVLSAVCVWHREKEYTALSTSCVTFRPLTEREIAQYWETGEPLGKAGAYAIQGRAAV
                     FIAKLEGSYSGVMGLPLYELADILQRIGSLNK"
     gene            4286..>4427
                     /locus_tag="AC16FV_08480"
     CDS             4286..>4427
                     /locus_tag="AC16FV_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850127.1"
                     /translation="MSEEILINVTPQETRVAVVENGVLQEVYIERARSRGIVGNIYKG
                     KVV"
BASE COUNT          899 a         1272 c         1419 g          837 t
ORIGIN      
        1 cgtttcagcg gcggcgaagt gggtctggat gacatcgagg ataatctcag cgacaatgcc
       61 cgttacaatg tttttgcgct ggcggacgcc agcctgcgcg gcgaaccggc gacggtcgaa
      121 cggattctca ccaacctgcg cggcgagggt gtcgagccga ttctggttct gtgggcgctg
      181 gcgcgtgagg cgcgcgaatt ggcgcagatg gcaagcctgg tcgcctccgg acagccggtg
      241 atgcgagtcc tcgatgcgcg acgggtgtgg gccaagcgca agccgctggt cagcgccgcg
      301 ctcaagcggc tgtcgccgga aacatcgcag gatctgctgc ggcaggccgc ccggacggac
      361 cgcattctga agggccgggg aaccggcgat gtgtggcagg aattgcagtg tctcgcgctc
      421 agtatgagcg gcctcaagcc cgcgacatgt catatataga tggtgagtac gacaaatagt
      481 agagattcat gaaagcagcc tcgaaacaga ccaccgacgc cgacatcaag cgttacatgc
      541 aggaagtcgg gcaacgcgcg cgcaccgcgg cgcgtatgat ggcgcgcgcc gagacggcgg
      601 ccaaggatgc tgccctgatg gcgatggccg ccgagatcga acgtcaaacc gacgcgctga
      661 tcgcggccaa caagaaggat atcgacgctg gcaaggtcca gggcctcgat tccgccatgc
      721 tcgaccgcct tgaattaaac ggcgcgcgca tcaaggccat ggccgagggc ctgcgccaga
      781 tcgcggcgct ggccgatcca gtgggcgaga tcaccgattt gaactaccgt ccttccggca
      841 tccaggtggg gcgcatgcgc gtgccgctcg gcgtgatcgg catcatttat gaatcgcgcc
      901 cgaacgtcac cgccgacgcc gccgggttgt gcctgaaatc cggcaatgcc tcgatcctgc
      961 gtggcggcag cgaggcgatt cactcgaacc gcgccattgc cgcctgcatc cagatcgggc
     1021 tcaagcaggc cggcctgccg gaagacgccg tgcaggtggt ggaaaccacc gaccgcgccg
     1081 ccgtgggcga gttgatccgc atgaaggaat tcgttgacgt catcgtgccg cgcggcggca
     1141 agtcgctcat cgagcgcatc agcgccgagg ccactatccc catcatcaag catctgcacg
     1201 gcgtgtgtca cgtggacatc gacgacaagg ctgatctcga caaggccgtg aaggtggcgt
     1261 tcaacgccaa gacccagcgc tacggcacct gcaacaccat ggaaaccctg ctggtcgcgc
     1321 gtggcgtggc ggcgacggtc ctgccggtgc tgggcaagat gtatcaggat aaaggcgtcg
     1381 aactgcgcgg ctgcgaggcc acgcgcaaga ttcttgccgg catcaaggcc gcgacggaag
     1441 aggattggca cacggaatat ctggcgccga ttctttccat ccgcgtggtg gacaacgtgg
     1501 atgaggccat ggatcacatc gcgcgctacg gctcggcaca taccgactcc atcgtgaccg
     1561 aggacatcac ccgcgcgcgc cgtttcctgc gcgaggtgga ttcgagttcg gtgatggtca
     1621 acgcctcgac gcgcttcgcc gacggattcg aatacggcct cggcgccgag atcggtatct
     1681 ccaccgacaa gttgcatgcg cgcggcccgg tcggtctcga ggggctgacg tcggtgaaat
     1741 tcatcgtgct cggggacggg catataagaa aatagacagg attcacagga ttaatttgat
     1801 tggaaattct tctttgtgtg ttctgattcc gcgaaatcct gttaatcctg tctattgtat
     1861 tttttgtggg taaacccctc ggcattcttg gcggcacatt cgatcccatt cactacggcc
     1921 atttgcgccc cgcgcaggaa gtcctgcgtg cgctcgatct gtcagagata cgaatcatcc
     1981 cggccgcgaa tccgccgcat cggcgtgcgc cgctggccac ggcggagcag cgtttgcgca
     2041 tggccgaact ggcggtggcc gaatttcccg gtttacgcgt ggatgatcgc gaactccggc
     2101 gcggcgggcc ttcgtacacc gtgttgacgc tggaatccct gcgccgcgaa ttgggcgcgc
     2161 agccgctgtg cctgttgctt ggcgtggacg ccttcgaggg catcgaaacc tggcatcagt
     2221 ggcagcggct cccggagttg gcgcattttg tcgtgatgac gcggccgggc tggccaccac
     2281 cggcggccga tgttctgcca tcgtgggcgc gcggccgtct cgcgcgtgtt ccggacgatc
     2341 tcgcccaagc cggtgcggga aagatttatt ttcaggccgt gacaccccag gatatttccg
     2401 cgacgcgtat ccgcgaagcc atcgcgcgcg gcgagccggt ggacgggctg ttgccgcccg
     2461 ccgtgctcga gtatcttcgc gccaaccgga tttatctcaa tcagggaaac tgatgccgaa
     2521 agccaccacc accaggaaaa cccaaagctg gattcaggac gcgctggaag acgccaaggc
     2581 gcgcgatatc accgtgctcg acgtgcgcaa gatcagcgat ttcaccgatt acatggtgat
     2641 cgccaccggc acctcgaatc ggcatgtgca gtccacgtcc gacaagatcg tggacgcgtt
     2701 gcgcagccac ggcgtgcgcg ccgtgggcgt cgaaggcggg aaaatcggcg actgggtgct
     2761 gatcgacttc ggcgacgtcg tggtgcatgt catgcgcgag gaaacgcgcg atttctacaa
     2821 cctcgaaaaa ctctggtccg acgccaaacg tgtcgagaca ggtcagaaaa agaaaaccgc
     2881 caaggcgcca agaacgccaa gaaaattgaa agtgaaaagc gggtcaggtt aatcaaaaag
     2941 ggcatacacc ggaagatggt tccgagtgat tcaagttact tttaattttt attccttggc
     3001 gctcttggcg tcttggcggt ttaatctcat atgcaaattc acatcatcgc catcggcgac
     3061 cgcatgcctg aatgggtcga ggcgggatac cgggagtacg ccaagcgcct gccgcgcgaa
     3121 tgccgtttgg tgctgcacga gatccccgcc ggccggcgca ccaagggcgc cgatctgcgc
     3181 cgcctcatcg aacaggaagg ggcgcgtcag atggacgcca ttcccgcggg cgcgcatgtg
     3241 gtggcgctcg accgcaccgg aaaacaactc gataccgaga cattggcggc ggaactcaaa
     3301 aagcggttgg gcggcggcga gcacctggcc ctgctgatcg gcggtccgga agggttggca
     3361 ccgccctgcc tggaacgcgc gaaagaacgc tgggcattgt ctaaacttac tctggcgcac
     3421 cccgtggtac gcgtggtttt ggtcgagcag ctttatcgcg cctggagcat tattcagaat
     3481 ctgccttatc atcggagata gatttccgat ttcaaatttc ctgttccgga tcgggcctgt
     3541 gtggactggc tgaagccggg attggaaatc tgaaattaga aatccggact tcaagatgat
     3601 ttatctcgcc tctgcttctc cccgccggca ggaattgctg cgccagctgg ggatcgagtt
     3661 cgaagtgatg ccgtccaata tcctcgaggt tcgtgaggcc ggcgaggcgc ccgcgcaata
     3721 tgtgacgcgt attgcgcgcg acaaggcgcg ctgggtggcg cagctggtca gggaacgcga
     3781 gttgccggcg catccggtgc tgggcgcgga cacggaggtg gtgctggacg gagaaattct
     3841 gggcaagccg gaaaacgctg cgcatggcat ggaaatgctg cggcgcctgg ccggacgcac
     3901 acatgaagtg ttgtccgccg tgtgcgtatg gcatcgcgag aaagaatata cggcgctgag
     3961 tacgagttgc gtgacgttcc gcccgttgac ggaacgagag atcgcgcaat attgggaaac
     4021 cggcgaacct ctgggcaagg ccggcgctta tgccattcag ggccgcgcgg cggtatttat
     4081 cgcgaaattg gagggcagct actccggcgt gatgggcctg ccgctgtatg agctagctga
     4141 tatcctgcag cgcatagggt ctctaaataa atgaaaatga ttttgaaccg ccaagacgcc
     4201 aagaacgcca agttttatga acttgagaat cgcctttctt ggctgtcttg gcgtcttggc
     4261 ggttgatctt tttaattaga aagacatgag cgaagagatc cttatcaacg ttaccccgca
     4321 ggagacccgc gtcgccgtcg tggaaaacgg cgtgctgcag gaagtctaca tcgagcgcgc
     4381 gcgcagccgc gggatcgtcg gcaatatata taaaggcaag gtggtcc
//
LOCUS       DBNGZE010000327         3722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1436148, whole genome shotgun sequence.
ACCESSION   DBNGZE010000327 DBNGZE010000000
VERSION     DBNGZE010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1436148"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="AC16FV_08485"
     CDS             complement(<1..1199)
                     /locus_tag="AC16FV_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2850128.1"
                     /translation="MELTTATNFIPLNALDVTYLLVVGFIGGLVSGFIGSGGAFVLTP
                     AMMSMGVPGIVAVASNICHKFPKALVGAIKRAKYGQVDVKLGLVLGVSAEAGVLYGAH
                     IQEAIHRAFGDAGSNLYVSLAFVIILGIIGSYVLWDAMKTRDSADSQQEKTARLARWV
                     QSVNIPGTMVYFKSLNARVSVLFTLPLGFATGMLAATIAVGGFIGVPAMHYVLGAPSL
                     MASASELVIAFVMGLGGTMKFAWGGLVDIRLAMIILAGSLFGIQLGAIGTTYVKPHMI
                     KMVMGVIMVLVLVSRAIVIPVYLSELKLIDELAPHLPGALKNISFVVLIAALAAGAAI
                     VFSALWRGISLQRREAALAGTGVTAFAPDVVATPAAAQLSPVGRFERFLLATDGSEYS
                     AGAVREAV"
     gene            complement(1208..1447)
                     /locus_tag="AC16FV_08490"
     CDS             complement(1208..1447)
                     /locus_tag="AC16FV_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850129.1"
                     /translation="MTKTSLDLAGKQVTRAALFGLVSVVLYFLLYLFEDEILEFTSQG
                     GWYFIAPVAIAFLISFVHGNFTAHFWEVLGIKAKK"
     gene            complement(1531..2346)
                     /locus_tag="AC16FV_08495"
     CDS             complement(1531..2346)
                     /locus_tag="AC16FV_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR2850130.1"
                     /translation="MTNPVMNFLALALYFIVGGLLGLRLMRGEVVTGGVKIGIFSLGL
                     GAVVLHAALLYTGLWSTSGLNLALTPAFSLVAWVVAVLYLATSLIRPVDNLGILIMPI
                     AGLTLLVEWLWPGQMSLALTSSVSAAHIIVSVLAYSLLCLAAVQSLMLLTQERHLRGR
                     HPGGFIRALPPVQTMESVMFQMIGLGFILLTLTLISGVFFSEAIFGRPLKFTHHMVLA
                     VLAWIVYAILLVGRWRMGWRGRSAIHWTLGGFALLLLAYFGSKFVLEVLLKRV"
     gene            2540..3304
                     /locus_tag="AC16FV_08500"
     CDS             2540..3304
                     /locus_tag="AC16FV_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2850131.1"
                     /translation="MKIRILGASGGIGAQARTTSLLVDQDVLIDAGTGVGDLTLPELR
                     AIDHVFLTHSHLDHVTMLPFLLDTVGADRERPVKVHAQEATLDTLRRHLFNGELWPDF
                     SRIPTPEAPFMTFQTLPVGGEVKLNGRTLRSIPVNHVVPAVGYLARGAKGSLAFSGDT
                     TVMDEFWKVLNATDDLRHVIIETSFMDEDAELSRISKHLCPSMLAGELAKLKSAAQVH
                     ITHLMPGREDDIMRQIARHIPHNTPRRLERGHVFEL"
     gene            3412..>3722
                     /locus_tag="AC16FV_08505"
     CDS             3412..>3722
                     /locus_tag="AC16FV_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR2850132.1"
                     /translation="MFDNLSQRLQVTLKRLRGQGRLTEENTREALREVRMALLEADVA
                     LPVVKDFIDHVRAKAAGQEVLASLTPGQALIKVVHDELVALMGAANEKLNLAARPPAV
                     IL"
BASE COUNT          800 a         1108 c         1166 g          648 t
ORIGIN      
        1 acggcctcgc gtacggcgcc ggcgctgtat tcagaaccgt cggtggcgag cagaaagcgt
       61 tcgaagcgcc cgaccggcga gagttgggcg gcagctggtg tggctaccac gtcgggcgcg
      121 aacgccgtga ctcctgttcc ggccagcgcc gcctcgcggc gctggagact gatgccgcgc
      181 cacagggccg agaatacgat cgccgcgcca gccgccagcg ccgcgatcag caccacgaag
      241 ctgatgttct tgagcgcgcc ggggaggtgc ggcgcaagct cgtcgatcag tttcagctcc
      301 gagagataga cggggatgac gatggcgcgt gaaaccagca ctagcaccat gatcacgccc
      361 atgaccatct tgatcatatg cggcttgacg taggtcgtac cgatcgcgcc gagctggatg
      421 ccgaacagcg agccggcgag gatgatcatc gccaggcgga tgtcgacgag tccgccccag
      481 gcgaatttca tggtgccgcc gagccccatg acgaaggcga tcacgagctc ggaggccgag
      541 gccatgagtg aaggcgcgcc caggacgtaa tgcatcgccg gcacgccgat gaacccgccc
      601 acggcgatcg tggccgcgag catgccggtg gcgaagccga gcgggagcgt gaacagcacc
      661 gacacgcgcg cattcaggct cttgaagtaa accatcgtcc cgggaatgtt gacggactgg
      721 acccagcgcg cgaggcgggc ggttttctcc tgctggctgt cggcggaatc ccgcgtcttc
      781 atggcgtccc acagcacgta gctgccgatg atgccgagga tgatcacgaa cgcgaggctg
      841 acgtacagat tggagccggc gtcgccgaaa gcgcggtgga tggcttcctg gatgtgcgcg
      901 ccgtaaagca cgcccgcctc ggccgagacg ccgagcacca gcccgagctt cacgtccact
      961 tggccgtatt tggcgcgctt gatcgcgccg accagcgcct tggggaactt gtggcagatg
     1021 ttcgaggcga ccgcgacgat gccaggcacg cccatgctca tcatcgccgg cgtgagcacg
     1081 aaggcgccgc ccgagccgat gaaaccgctg accagtccgc cgatgaagcc gacgacgagg
     1141 agataggtga cgtcgagcgc gttcagtggg atgaaattcg ttgctgttgt cagttccacg
     1201 ccgcccccta ttttttcgcc ttgatgccga ggacttccca gaagtgggcg gtgaagttcc
     1261 cgtgcacgaa ggaaatcagg aaggcaatgg ccacgggcgc gatgaaatac cagccgccct
     1321 gcgaggtgaa ttcaaggatc tcgtcttcga aaagataaag caggaagtaa agtaccaccg
     1381 acaccagccc gaaaagcgcg gcacgcgtaa cctgtttgcc ggccaggtcc aaagatgttt
     1441 ttgtcatacc aaacccccca ttgttggtgt gcgaaattgg aaccgattgt tatgtgcgag
     1501 tccaattctc tttctcttct tgaagcggct tcatacgcgt ttgagcagta cctccagcac
     1561 aaacttgctg ccgaaatagg ctagcagcag cagcgcaaaa ccgcccaggg tccagtggat
     1621 ggcggagcgc ccgcgccagc ccatgcgcca gcggccgaca agcaggatcg cataaacaat
     1681 ccaggcgagc accgccagca ccatgtgatg tgtgaacttg agcggccgac cgaaaatcgc
     1741 ctcggagaag aacacgccgc tgatcagcgt gagcgtaagc agaatgaagc cgaggccgat
     1801 catctggaac atcacgcttt ccatggtctg caccggcggc agggcgcgga tgaatccgcc
     1861 gggatgccgg ccgcgcaggt ggcgctcctg cgtgagcaac atcaggcttt gcaccgcggc
     1921 cagacacagc aggctataag ccaggaccga cacaataatg tgcgccgccg agacgctgga
     1981 cgtcagcgcc aacgacatct ggccgggcca cagccactcg accagcagcg tgaggccggc
     2041 gatgggcatg atcaggatgc cgaggttgtc gaccggacga atgagcgagg tggcgagata
     2101 cagcaccgcc acaacccagg caaccagcga gaacgcgggc gtcagggcca gattgaggcc
     2161 ggacgtggac cacagaccgg tgtagagtag cgcggcgtgc agcaccaccg cgccgagtcc
     2221 gagactgaag atgccgatct tgacgccccc cgtgacgacc tccccgcgca tcaggcgcag
     2281 acccagcagt ccgccgacga tgaaatacag cgccaaggcg aggaaattca tgacaggatt
     2341 ggtcataata tacgttgtct acttattatg ttcgtctgat cgtagaaccg gtcgacctac
     2401 ttcgttcgtc tggtcataga aaatgtcgac ctgctgcgct cgtctggcca taatatcagc
     2461 gcatgatggc acaggttgtc tcacctttga agagcagacg cagcgtgaat gagcattttt
     2521 gtcagcggat aagccataga tgaaaatccg gattctgggc gccagcggcg gtatcggcgc
     2581 gcaagcgcgc acgacatcat tgctggtgga tcaggatgtc ctgatcgacg ccggcactgg
     2641 cgtgggcgat ctgacactcc cggagttgcg cgccattgat cacgtgtttc tgacccactc
     2701 gcatctcgat cacgtgacca tgctgccgtt cctgctggat accgtgggtg cggaccgcga
     2761 gcggccggta aaggtgcatg cgcaggaggc aacactggat accttgcgcc ggcatctttt
     2821 caacggcgag ctctggcccg acttcagccg catcccgacg ccggaagcgc cgttcatgac
     2881 atttcaaaca ctgccggtgg gtggcgaggt caaattgaac ggaaggaccc tgcgcagcat
     2941 accggtcaat cacgtggttc ccgccgtggg ctatctggcg cgcggcgcga agggtagcct
     3001 cgcattcagc ggcgacacta ccgtgatgga tgaattctgg aaggtgctca atgcgaccga
     3061 tgatctccgg cacgtgatca tcgaaacgag tttcatggat gaagacgcgg agctgagccg
     3121 gatttccaaa catctgtgcc cctcgatgct cgcgggcgag ctcgcaaaac tgaaatccgc
     3181 tgcccaggtc cacattacgc atctgatgcc cgggcgcgag gatgacatca tgcgccaaat
     3241 cgcgcggcat atcccgcaca acaccccacg ccgcctcgaa cgcggtcatg tatttgagtt
     3301 atagaattgt gaaccgccag ggcgccaggg tcgccaaaag agaaaatttt ttgattgtga
     3361 ttttcctggc gttcttggcg tcctggcggt ttaaaattat tattgattac aatgtttgac
     3421 aatctaagcc aaagattaca ggtcaccctc aagcgcctgc gtggtcaggg gcggctcacc
     3481 gaggagaaca cgcgcgaggc cctgcgcgag gtgcgcatgg cgctgcttga ggccgatgtg
     3541 gccctgccgg tggtgaagga cttcatcgac catgtgcgcg ccaaggccgc cggtcaggaa
     3601 gtgctcgcga gcctgacgcc gggacaggcg ctgatcaagg tcgtgcacga cgagctggtc
     3661 gcgctcatgg gcgcggccaa tgaaaaactc aatctggcgg cgcgtccgcc ggcggtgatc
     3721 ct
//
LOCUS       DBNGZE010000328         4747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_185454, whole genome shotgun sequence.
ACCESSION   DBNGZE010000328 DBNGZE010000000
VERSION     DBNGZE010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_185454"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16FV_08510"
     CDS             complement(<1..225)
                     /locus_tag="AC16FV_08510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850133.1"
                     /translation="MAEQSGTRLPVSAVIITRDAASQIEPCLQSVSFADEILVVDSGS
                     TDATLEIARRYGARVIHQDWLGYGKQKEFAA"
     gene            253..1218
                     /gene="waaC"
                     /locus_tag="AC16FV_08515"
     CDS             253..1218
                     /gene="waaC"
                     /locus_tag="AC16FV_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR2850134.1"
                     /translation="MRLLIIKTSSLGDVIHNLPAVSDIVSHIPGAEIDWVVEESFAEI
                     PRLHPKVCRVIPVALRRWRRQMLSPATWREVSRFRRELKAETYDKIIDTQGLVKSAWL
                     ARMARGPSYGQDKATAREWLAASFYDHHLHVARGRHAVERNRDLVAQALGYPLPQTPP
                     DYGIRAPQASFSFTVPGKFMIGLHATSRETKRWPMEHWVALGRELDGRGITLLLPWGS
                     AEEEQHAHQISQSLKNATVLPRLGLRELAALMGRAQAVVGVDTGLVHLAAALGRPTVA
                     IYTDTSPTLTGVVAADANRARNLGDVNRVPEPEAVKVALAELGSL"
     gene            1218..2486
                     /gene="waaA"
                     /locus_tag="AC16FV_08520"
     CDS             1218..2486
                     /gene="waaA"
                     /locus_tag="AC16FV_08520"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR2850135.1"
                     /translation="MRVIYIWLLRLLAPYVLLRLMWRGFSNPDYWRRIPERFGFIEPL
                     TLPRVIWIHAVSVGEVRAAAPLVHELAVRYPEYPLLITTMTPTGSAQVRELFGGSVAH
                     CYAPYDYPSAVKRFLDRTQPVMTIVMETELWPNIFHQCRTRGMPVFVTNVRMSESSMR
                     KYRRFASLARATLQQVTRFAAQTRTDAQRLLTLGAAPETVVVTGSIKFELNIGASMRE
                     AAEVLRITWGQDRPVWLAASTHEGEEEMVLDAFARLKKQARFANMLLVLVPRHPERFA
                     AVARLCKKNYRIALRSENRGGLDPAVEILVGDTMGELQLFYGAADVAFIGGSLVPTGG
                     HNLLEASAVGKPVVFGPHMFNFQEISQMTLERGAGVQIQFPEQLAPTVSDFLGNANRR
                     DSAGLAGRKMVEENRGALTANMRLIEELLK"
     gene            complement(2618..3034)
                     /locus_tag="AC16FV_08525"
     CDS             complement(2618..3034)
                     /locus_tag="AC16FV_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR2850136.1"
                     /translation="MSETRYETPQEAEAAFYAAFIKRDVNAMMEVWAADDNISCIHPL
                     GPILVGRVAIRESWETIFRNSPDMQIMINGRSRGRDGSFAIHVVEEHIRSGNEPPGTP
                     MHATNVYHLTDTGWRMVLHHASPSPPPSKTDTPTLH"
     gene            complement(3058..3378)
                     /locus_tag="AC16FV_08530"
     CDS             complement(3058..3378)
                     /locus_tag="AC16FV_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2850137.1"
                     /translation="MQQMSVQELKEKLDKGTKDMVVVDVREPWEVNVCSLPGAVTIPM
                     RAIPARYLELPKDAEIVVMCHHGIRSQQVAYYLERMGFNKLNNLAGGIAAWARDIDPK
                     MPTY"
     gene            complement(3389..4051)
                     /locus_tag="AC16FV_08535"
     CDS             complement(3389..4051)
                     /locus_tag="AC16FV_08535"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR2850138.1"
                     /translation="MSSLNFDVARRNMIECQIRPWDVLDERILELVARLPREDYVPKQ
                     YRNIAFVDMNIPLGHGEVMVAPKLEARLAQELEINHSDKILEIGTGSGYMTALLAALG
                     RHVVSVEIRAEFTAEAATKLAHHGVRNITLETGDGARGWDRQKPYDVILLTGSTPILP
                     EAFQQSLAVGGRMIAIVGTAPAMDVRRIRRLGEHSFEQKSLFETVIPKLQNALEPARF
                     VF"
     regulatory      4378..4475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4529..>4747
                     /locus_tag="AC16FV_08540"
     CDS             4529..>4747
                     /locus_tag="AC16FV_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779727.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR2850139.1"
                     /translation="MSANPKDFLQPTSTLSAEAIRPFPAARKIYQQGSRPDLRVGMRE
                     VTLTPTPSTHGAEENPPITVYDTSGPYTD"
BASE COUNT          864 a         1477 c         1465 g          941 t
ORIGIN      
        1 cgcggcgaat tccttctgtt tgccatagcc cagccagtcc tggtgaatga cccgcgcgcc
       61 gtaacggcgc gcgatctcga gcgtagcatc ggtactgccg gagtcgacga ccaggatttc
      121 atcggcaaag ctgacgcttt gcaggcaggg ttcgatctgg gaggccgcgt cgcgggtgat
      181 gatgacggcg gaaacgggga gcctggttcc ggattgttct gccatgatgc cattctatgc
      241 gaaaatcttc gcgtgcgttt attgataata aaaacctcct cgctcggtga cgtgattcac
      301 aacctgccgg cggtctccga tatcgtttct catatacccg gcgccgagat cgactgggtg
      361 gtggaagaaa gctttgctga aattccgcgg ctgcatccca aagtgtgccg ggtgattccc
      421 gtggcgctgc gccgctggcg ccgccagatg ctcagcccgg ccacgtggcg cgaggtctcc
      481 cggtttcgcc gcgagctgaa agctgaaacc tacgacaaga ttatcgatac ccaagggctg
      541 gtgaaaagcg cttggctggc gcgcatggcg cgcggcccgt cgtatgggca ggacaaggcc
      601 acggcgcgcg agtggctcgc ggccagtttc tacgatcacc atttacatgt cgcacgcggc
      661 cggcacgcgg tcgagcgcaa ccgcgatctc gtggcacagg cattgggata ccctttgccg
      721 caaactccgc ccgattacgg catccgcgcg ccgcaagcgt cgttttcctt caccgtccca
      781 ggaaaattca tgatcggcct gcacgccacc agccgcgaaa ccaaacgctg gccgatggaa
      841 cattgggtcg cgctcggacg ggaactcgat ggtcgtggta tcactctcct gctgccgtgg
      901 ggcagcgccg aggaagaaca gcatgcccac cagatttccc agtcgctgaa aaacgccacg
      961 gtgttgccgc gcctcggcct gcgcgaactc gccgcgctca tgggccgtgc gcaggccgtg
     1021 gtcggcgtgg acaccgggct ggtgcatctc gccgccgccc tcggtcgccc gacggtggcg
     1081 atctataccg atacctcgcc gacactgacc ggcgtggtcg ccgccgatgc caaccgtgcg
     1141 cgcaatctcg gcgacgtcaa tcgcgtaccg gaacccgagg ccgtcaaagt ggcgctggct
     1201 gagctaggct ccttgtaatg cgtgttatct acatctggct tctgcgtctg ttggcgccgt
     1261 atgtgctgct gcgcttgatg tggcgcgggt tcagcaatcc ggattactgg cggcgtatcc
     1321 ccgaacgttt cggattcatc gagccgctga cgctgccgcg cgtgatctgg attcatgcgg
     1381 tgtcggtggg cgaggtgcgc gctgccgcgc cgctggtgca cgaactcgcc gtgcgttatc
     1441 ccgaataccc gttgctgatc accaccatga cgccgaccgg ctcggcgcag gtgcgcgagc
     1501 tgttcggcgg cagtgtcgcg cattgctacg cgccctacga ctacccttcg gcggtcaagc
     1561 gctttctcga tcgcacccaa cccgtgatga cgatcgtcat ggaaaccgag ctgtggccga
     1621 acatctttca tcagtgtcgc acgcgcggga tgccggtgtt cgtcaccaac gtgcgcatgt
     1681 ccgagtcctc gatgcgcaag taccggcgct tcgccagcct ggcgcgcgcc acgttgcagc
     1741 aggtgacccg tttcgcggcg caaacccgga cagacgcgca acgcctgctc acactcggcg
     1801 ccgccccgga aaccgtggtg gtgaccggca gcatcaagtt cgagctcaat attggcgcca
     1861 gcatgcgcga ggctgctgaa gtgctgcgca ttacctgggg ccaggaccgc ccggtgtggc
     1921 ttgcggccag cacccacgaa ggcgaggagg agatggtgct cgacgcgttc gcacggctta
     1981 aaaaacaggc gcgcttcgcc aacatgctgc tggtgctggt gccgcgccac cccgagcgct
     2041 ttgccgccgt ggcccggctg tgcaagaaga actatcgcat cgcgctgcgc agcgagaacc
     2101 gcggcgggct cgatcccgcg gtcgaaatcc tcgtcggcga caccatgggc gagctgcaat
     2161 tattctatgg cgcggccgac gtagccttca ttggcggcag cctggtgccc accggcggcc
     2221 acaacctgct tgaggcctcg gccgtgggca agccggtggt gtttggccca cacatgttca
     2281 acttccagga aatcagccag atgacgcttg agcgcggcgc cggggtgcag attcagtttc
     2341 cggagcagct ggcgccgacg gtgtcggatt ttctcggcaa cgccaaccgc cgcgacagcg
     2401 ccggccttgc cggccgcaag atggtggagg aaaaccgcgg cgctctcacg gccaacatgc
     2461 gcctgattga agaattattg aaataggctg ggaccgcaga tgaacgcaga ttaaattcag
     2521 atcaaattga ctaaaaataa cacttagaat ctattttttt gtcttatctg cgttcatcat
     2581 tttcaaatca tctgcgtcga tctgcgttcc agaaatatta gtgcaacgtc ggtgtgtcgg
     2641 tcttcgaagg cggcggggag ggcgaagcgt ggtgcagcac catgcgccag ccggtgtcgg
     2701 tgagatggta aacgttggtc gcgtgcatcg gcgtgcccgg cggttcatta ccgctgcgga
     2761 tgtgttcctc cacgacgtga atcgcgaacg atccatcccg tccgcgcgag cggccgttga
     2821 tcatgatctg catgtccgga gagttgcgga aaatcgtctc ccaagattcg cggatggcga
     2881 cgcgcccgac caggatcggg ccgagcggat ggatgcagct gatgttgtcg tccgcggccc
     2941 acacctccat catcgcgttc acgtcgcgct tgatgaaggc ggcatagaag gcggcctcgg
     3001 cttcctgcgg ggtttcgtag cgggtctcac tcatggcagc caaccgttgt cgcgcactta
     3061 gtaggtcggc atcttcgggt cgatgtcgcg cgcccaggcc gcgatcccgc cggcgaggtt
     3121 gttcagcttg ttgaacccca tgcgctcgag ataatacgcc acctgttggc tgcgaatccc
     3181 atgatggcac atcacgacga tctcggcgtc cttgggcagc tccaggtagc gcgccgggat
     3241 cgcgcgcatg gggatggtga ccgcgccggg cagcgaacag acattcactt cccacggctc
     3301 gcgcacatca accaccacca tatccttcgt ccctttgtcg agcttttcct tcagctcctg
     3361 gacggacatt tgctggatca tcggaatctc aaaacacgaa tcgggcaggt tcgagcgcgt
     3421 tttgcagctt cgggataacc gtttcgaaca gcgatttctg ctcgaaggaa tgttcgccga
     3481 gccggcggat gcgccggacg tccatcgccg gcgccgtacc cacgatcgcg atcatacgcc
     3541 cgccgacggc gaggctctgc tggaaggcct cgggcaggat cggcgtcgag ccggtgagga
     3601 gaatcacatc gtaaggtttc tggcggtccc agccgcgcgc gccgtcgccg gtctccaaag
     3661 tgatgttgcg cacgccgtgg tgcgcgagtt tggtcgcggc ttcggcggtg aattcggcgc
     3721 ggatctcgac gctcaccacg tgccggccga gcgccgccag cagcgccgtc atgtagccgc
     3781 tgccggtgcc gatctcgaga atcttgtcgc tgtggttgat ctccagttcc tgcgccaggc
     3841 gcgcctcgag tttgggcgcc accatgactt cgccgtggcc gagcgggatg ttcatgtcga
     3901 cgaaggcgat gttgcggtat tgcttgggca cgtagtcctc gcgcggcagg cgggcgacca
     3961 gttcgaggat gcgctcgtcg agcacatccc acggccggat ctggcactcg atcatgttcc
     4021 ggcgcgcaac gtcaaaattc agcgaagaca taaaactact cggagtaggg ccaaagggct
     4081 gcctagccta agcagtttgc ccaaagcgcg caagccgagg gcctttatgt ggatattcag
     4141 gaaagcttca attttatgtg ccgtgattcc cgcccccgac taaaacattc gagggcaggc
     4201 ttcagcggga accaggctgg agtagacagg gcgctgaaat tattgtcatt cctgcgaaag
     4261 cgggaatcca gtgatttcat ataaatgctc ttgatattaa ttttctgggc ccccggctcc
     4321 gcgggggcga cttcgtcggt tgcaatcccc ccgggaatcc tttagcgtga aatcatctgt
     4381 atgagctagg ggtgctggcc cagtggccgg ctgagagatc acccttcgaa cctgaaccgg
     4441 ttagtcccgg cgtaggaaag cttcctgaag cctcctcagt tttgccccgc cgctcatacc
     4501 tccgatcagt tttaacgagg tagtcatcat gagcgccaac cccaaggatt ttctgcaacc
     4561 gacctcgacg ctttccgccg aggccatccg cccgttcccg gccgcgcgca agatttatca
     4621 gcagggctcg cgccccgacc tccgagtcgg tatgcgcgaa gtcacgctga cgcccacgcc
     4681 ctcgacccac ggcgccgagg agaacccgcc gatcacggtg tacgacacct ccgggccgta
     4741 caccgac
//
LOCUS       DBNGZE010000329         6816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1227213, whole genome shotgun sequence.
ACCESSION   DBNGZE010000329 DBNGZE010000000
VERSION     DBNGZE010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1227213"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="recJ"
                     /locus_tag="AC16FV_08545"
     CDS             complement(<1..1310)
                     /gene="recJ"
                     /locus_tag="AC16FV_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR2850140.1"
                     /translation="MADAKIVRRAPTGVEGFLTDLHPVLQRIYRARQVASREELEKSL
                     DRLISPAQLKGIDEASILLADALRRQRRILIVADFDADGATSCALAVRALRSLDATDV
                     RYLVPNRFEFGYGLTPEIVALAAEQKPDLIITVDNGISSIEGVKAARSRDIDVLVTDH
                     HLPGESLPAATVIVNPNQPLDSFPSKNLAGVGVIFYVMLALRSRLRASGWFTERNIAE
                     PNLGRLLDLVALGTVADVVPLDANNRILVAQGLKRINQGQACAGIDALLRIAGRQPQR
                     VTAMDLGYIVGPRLNAAGRLTDMSLGIECLLTDDAAAAHAMAEKLDALNRERRSIEAT
                     MQQQAVAEVESLRLDMVALPRGLCLFHESWHQGVIGIVAARIKERVHRPVIAFAPVSE
                     TEMKGSARSVPGLHIRDVLDAIAARHPHLLSKFGGHAMAAGLTL"
     gene            complement(1310..2377)
                     /locus_tag="AC16FV_08550"
     CDS             complement(1310..2377)
                     /locus_tag="AC16FV_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850141.1"
                     /translation="MTIIPDSPASQHLTLYVPGLFSLQENDRAESGSPRALETLLTRA
                     DMTDRDFPRGYEEGLFALFDLAPPENADLPVAAVTRMFDMGVIDNDWWLRADPVHLSL
                     DRDRLVLADARKLEITPEEAARLVAEIMEVFASDGWLLKAPHPERWYLKPTRAPRLTT
                     SLLSQVVGRDVRGFLPRGPDDKAWHTVLNEIQILLHTATVNVEREKAGKLPINSLWFW
                     GSGRLPRITPVTWTHVWSNEPVSLSLAKLSGTPATRLPDNYADWRRQAGTIGEHLVVL
                     DEAHDATLYNDAPRWNEILHTLETQWMQPLLRELKSGVIDRVSLLSDTGACFTLTSRQ
                     ARRWWRPRRTLSSHRERNGQP"
     gene            2699..4726
                     /locus_tag="AC16FV_08555"
     CDS             2699..4726
                     /locus_tag="AC16FV_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR2850142.1"
                     /translation="MSSKTGVSTAKENVASDDGKVVAMDDFVQSRNAKAALDGFARGL
                     TQFKRWRDDLAQAIADYQTWVEQQGFSDGEQDLRVYELIEMLQSDKLTVALVAEFSRG
                     KTELLNAIFFSDYRQRLLPSAAGRTTMCPTELRWDEKDGPCVKLLPIETRKTALTISE
                     YKRTPVHWTAIHILKLSSAEEVREALSELTKTKVVSRREAQELGLYDPKQPENKNAPV
                     SGDQVEVPVWRHAIVNFPNALLKQGLVVLDTPGLNALGAEPELTLNTLPSAQAILFLL
                     AADAGVTKTDLDVWTNHVSTVRGNSHMVALNKIDILWDELHDDAAVAKTIARQVEETA
                     RTLHIDRKAIFPVSAQKGLIAKIKGDAAMVAKSGLSALEDKLTQDLIPARQMLIRNRI
                     VHEVSGRVESSRALIESKLAGVNKQLGELKALSGKNQEAIQKMIQRMRVEKQKYDKEI
                     EGFQLTKAALSKQAQILLGHLSMAVLDELIAKTRVAMNETWTTRGLKNGMETFFTGTR
                     ERMEKVSYESDEIRKVVEAIYLRLHTEYGLAKIVPPRLSLLPFVMEFKKLEERADAFR
                     DSPVTMMTEQHFVIKKFFITLVSQARQLFNECNTASKNWFQAAVAPVFAQIQQHKATI
                     DRSFEALKKVHENMDSLGERIAELETVRKDLEDQMKTTDVLLERIHRPLVE"
     gene            complement(4746..5960)
                     /locus_tag="AC16FV_08560"
     CDS             complement(4746..5960)
                     /locus_tag="AC16FV_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850143.1"
                     /translation="MLFISALARAAGWDFGEKIPVAPVSDSRVFQHLDATGRKSMAMS
                     GNTLAVVWEDNRNGSPQAHIVFKATDKNEFSTAQRISSGKSAFGPAILALPNNRFLAG
                     WEQDNAVWVRQVSPHGLGPALKLSGKDATQIALATQDGKQVVAVWCQRAGKFNRVMAQ
                     ELQIGADGKVAQAGTIKPVDAKPPVDDQSYPTVIMTRKGTVVAWEDRRRQHSVLLYSH
                     ATPGDDFSAPQLLNEVVQKSDLYGRGSGVTRVALDVYGSDRIAATWMDKRGDQTGYDI
                     YAAFSEDGGRRFGTNELVQDSFADQYAQWHPAIAGDASGRVIVAWDDDRDDASSIWLA
                     WKTPDGWSTNFSPPVASGEGDKTSPSITLDAQGNLHLIWLEHGTDNGAERIMYVMGRN
                     SGEPSRPAQQSK"
     gene            complement(5984..6460)
                     /locus_tag="AC16FV_08565"
     CDS             complement(5984..6460)
                     /locus_tag="AC16FV_08565"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2850144.1"
                     /translation="MLAAWAAVFSNVSHADAPPGYPFLRYDEALKSARQQNKKIFVYY
                     GRYGCGFCDKTNKKSFSDPALKKLYTEHYVLTYVDAESGKRLVLPNGERITEMELGAR
                     LKAKVTPVFIYMDAEGKSLLKVPGFQTVQDFIKYDQYIHDGHHKNQTLDQFLAQTR"
     gene            complement(6613..>6816)
                     /locus_tag="AC16FV_08570"
     CDS             complement(6613..>6816)
                     /locus_tag="AC16FV_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2850145.1"
                     /translation="SAAVLVGRLGTNQIPYLMSAVLVAGVLIGAQLGSVMSQKTPRRL
                     LRGALSVLIAATALKIWYELLLA"
BASE COUNT         1390 a         2052 c         2054 g         1320 t
ORIGIN      
        1 tccagcgtca ggccggcggc catggcatgt ccgccgaatt tgctgagcag atgtggatgg
       61 cgcgcggcga tggcgtcgag cacgtcacgg atatgcaggc cgggaacgga acgcgccgat
      121 cctttcatct cggtttcgct gaccggggca aacgcgatca ccgggcggtg cacgcgctcc
      181 ttgatgcgcg cggccacgat gccgatcact ccctgatgcc aggattcgtg aaacaggcac
      241 agcccgcgcg gcagcgcgac catgtcgagc cgcaggctct ccacttccgc gacggcctgt
      301 tgctgcatcg tggcctcgat ggagcgacgt tcgcgattca gggcgtcgag tttttccgcc
      361 atggcatgcg ccgcggcggc gtcatccgtc agcagacatt cgatgccgag cgacatgtcg
      421 gtaaggcggc cggcggcgtt gaggcgcggg ccgacgatgt agccgaggtc catggccgtc
      481 acgcgttgcg gctggcggcc ggcgatgcgc agcaaggcat cgatgccggc gcaggcctga
      541 ccctgattga tgcgcttcaa cccctgcgcc accaggatgc ggttgttggc gtcgagcggc
      601 accacatcgg cgacggtgcc gagcgcaacc agatcgagca accggcccag attgggttcc
      661 gcgatattgc gctccgtgaa ccagccgctg gcgcgcaggc gcgagcgcag cgccagcatg
      721 acatagaaga tcacgccgac gccggcgaga ttcttgctcg gaaaagaatc gaggggctga
      781 ttgggattga cgatgaccgt cgccgccggc agcgactcgc cgggcagatg atgatcggtg
      841 accagcacgt cgatgtcgcg gctgcgcgcc gcttttacgc cttcgatgct ggaaatgccg
      901 ttatcgacgg tgatgatcag atcgggcttc tgttccgccg ccagcgcgac gatttccggg
      961 gtcaagccat agccgaattc gaagcggttc ggcaccaagt agcgcacatc cgtcgcatcc
     1021 aggctgcgca aggcgcgcac cgcgagcgcg caactggtgg ctccgtcagc atcgaaatcg
     1081 gccacgatga gtatgcgccg ttgtcgtcgc agcgcatccg cgagcaggat cgaggcctca
     1141 tcgatgccct tgagctgggc cggcgaaatc aaacgatcca gcgatttttc cagttcctcg
     1201 cgcgaggcca cctgccgcgc ccggtagatg cgctgcaaca ccggatgcag atcggtcaga
     1261 aaaccctcga ctccagtggg tgcgcggcga acgatttttg cgtcagccat catggttgcc
     1321 cgttcctctc gcgatgtgag gacagtgtac gacgcggtcg ccaccagcgg cgcgcctgcc
     1381 gtgacgtgag cgtgaagcac gcgccggtat cgctcagcag cgagacgcgg tcgatcacgc
     1441 cggatttcaa ttcacgcagc agcggttgca tccattgcgt ttccagcgtg tgaagaattt
     1501 cgttccagcg cggcgcatcg ttgtaaagtg tcgcatcgtg cgcttcatcc agcaccacaa
     1561 gatgttcgcc gatcgttcct gcttgccgac gccagtccgc gtagttatca ggcaggcgcg
     1621 tcgctggcgt gcctgatagt ttcgccaggg acaggctcac gggttcgttg ctccatacat
     1681 gcgtccaggt taccggcgtg atgcgcggga gccggccact gccccagaac cacaggctgt
     1741 tgatgggcag cttgccggcc ttctcgcgtt cgacattgac cgtcgccgtg tgcaacagga
     1801 tctggatttc attcaggacg gtgtgccagg ccttgtcgtc gggtccacgc ggcaggaacc
     1861 cgcggacgtc gcgcccgacc acttgtgaca gcaatgaggt cgtgagccta ggggcgcgtg
     1921 tcggtttgag ataccagcgc tccggatgcg gcgccttgag cagccatccg tcggaggcga
     1981 agacctccat gatttccgcg accaggcgcg cggcctcttc cggcgtgatt tccagtttcc
     2041 gcgcgtcggc gaggaccagg cgatcgcgat cgagactcag gtgcaccggg tcggcgcgca
     2101 accaccaatc gttgtcgatc acgcccatgt cgaacatgcg cgtgacggcg gcgaccggca
     2161 ggtcggcgtt ttccggaggc gcgagatcga acaaggcgaa caggccttct tcgtagccgc
     2221 gcggaaagtc gcggtcagtc atgtccgccc gggtcagcag cgtctccagc gcgcgcgggg
     2281 atccgctttc tgcgcggtca ttttcctgca ggctgaataa tccggggacg tatagggtca
     2341 ggtgctgact ggcggggctg tcagggatta ttgtcatttc tttatggctt gtcccgcatg
     2401 gcgtacgtca tcgccggatt ttatgtcata tccccttcgc acagaacgga acgcgggttg
     2461 cgccggaaaa caggaggata gccatgcgga cgccacgtcg ccccgaggcc tgaaatacgg
     2521 ccgttgccgg tatatgcggc cggtccgccc tcatcctggt tgcccagagg caaaaaacag
     2581 cccgaaaacc accccataaa ccactcgatt tcgcctgaaa atacaggtgt ggcccgactt
     2641 ttgcacaata gataaaggac taaatcagtt gtttcctgga attttcaggc agggtgccgt
     2701 gagctccaaa accggagtat cgaccgcaaa ggaaaacgtc gccagcgacg acggtaaagt
     2761 cgtggcgatg gacgattttg tccagtcgcg caacgccaag gccgcgctcg atggcttcgc
     2821 acgcgggctg acccaattca agcgctggcg cgacgacctc gcgcaggcga tcgccgatta
     2881 tcagacgtgg gtcgagcaac agggcttctc cgacggcgag caggatctgc gcgtctacga
     2941 gctgatcgag atgttgcagt ccgacaagct gaccgtggcg ctggtggccg aattctcgcg
     3001 cggcaagacc gaactcctca acgcgatttt cttctccgat tacaggcaaa gactactgcc
     3061 ctccgcggcc ggccgcacca ccatgtgtcc gaccgaattg cgctgggacg agaaagacgg
     3121 tccttgcgtc aagctgctgc cgatcgagac gcgcaagacg gcgctgacca tctcagaata
     3181 caagcgcacg ccggtacact ggaccgcgat tcacatcctg aagctgagtt ccgccgagga
     3241 agtgcgcgag gcgttgtcgg agctgacgaa gacgaaggtg gtcagccggc gcgaggcgca
     3301 ggaactgggg ctgtacgatc cgaaacagcc ggaaaacaag aatgcaccgg tttccggaga
     3361 ccaggtcgaa gttccggtat ggcgccatgc catcgtcaat tttccgaatg ccctgctcaa
     3421 gcaaggactg gtggtgctcg acacgcccgg attgaacgcg ctgggcgcgg agcccgaact
     3481 gacgctgaat acgctgccaa gcgcgcaggc gatacttttc ctgctggccg ccgacgccgg
     3541 cgtgaccaag accgatctcg atgtctggac caatcacgta agcaccgtgc gcggcaattc
     3601 gcacatggtg gcgctgaaca agatagatat tctctgggat gagctgcacg acgatgccgc
     3661 ggtggccaaa accatcgcgc gccaggtcga ggaaaccgcg cgcacgttgc atatcgaccg
     3721 caaggccatt ttcccggttt ccgcgcaaaa gggcctgatc gccaaaatca agggcgatgc
     3781 cgccatggtc gccaagagcg gcctgtcggc gctggaggac aaactcacgc aggatcttat
     3841 cccggcacgt cagatgctga tccgcaaccg catcgtgcat gaagtgagcg gccgggtcga
     3901 aagcagccgg gcgctgatcg aatccaagct ggccggcgtc aacaagcaac tgggcgagct
     3961 caaggcactg agcggcaaga accaggaagc gattcagaaa atgatccagc gcatgcgcgt
     4021 ggaaaagcaa aaatacgaca aggaaatcga gggcttccag ctgaccaagg ctgccctctc
     4081 caagcaggcg cagatactgc tcggccacct gagcatggcg gtcctggatg aattgatcgc
     4141 caagacgcgc gttgccatga atgaaacctg gaccacgcgc gggcttaaaa atggcatgga
     4201 aacgttcttt accggcaccc gcgagcgcat ggaaaaggtc agctatgaat cggatgaaat
     4261 ccgcaaggtg gtcgaggcga tttacctgcg cctgcatacc gaatacggac tggccaagat
     4321 cgtgccgccg cggctgtcac tgctgccgtt cgtgatggaa ttcaagaaac tcgaggagcg
     4381 tgccgatgcg ttccgcgaca gccccgtcac gatgatgacg gagcagcatt ttgtcatcaa
     4441 gaaattcttc atcaccctgg tcagccaggc gcgccaactg ttcaacgaat gcaacactgc
     4501 ttcaaagaac tggttccagg ccgccgtggc gccggttttc gcccagatcc agcagcacaa
     4561 ggcgaccatt gaccgcagct tcgaggccct gaaaaaggtt cacgagaaca tggacagcct
     4621 cggcgaacgt attgccgaac tggaaacggt tcgcaaggat cttgaagacc agatgaaaac
     4681 gaccgatgtc ctgctggaac gcatccaccg ccccttggtg gaataaaccg aagggcaata
     4741 tcagtttatt tcgattgttg cgccggacgt gacggctcgc ccgaattgcg ccccatgacg
     4801 tacatgatcc gctccgcgcc gttgtccgtt ccatgttcca gccagatcag gtgtagattg
     4861 ccctgtgcat ccagcgttat cgaagggctg gtcttgtcgc cttcgcccga cgccaccggt
     4921 ggcgaaaaat tcgtgctcca gccgtcgggg gttttccacg ccagccagat gcttgaagcg
     4981 tcgtcacggt catcatccca ggcgacgatg acgcgtccgg acgcgtcgcc ggcgatggcg
     5041 ggatgccatt gcgcgtactg atcggcgaaa gaatcctgca ccagctcatt agtaccgaaa
     5101 cgccggccgc cgtcctcgct gaaggcagcg tagatatcgt acccggtctg gtcaccacgc
     5161 ttgtccatcc atgtcgccgc gatcctgtca ctgccgtaca catcaagcgc gacgcgcgtt
     5221 acgccactgc cgcggccata cagatcggat ttctgcacta cttcattcag caattgcggc
     5281 gcgctgaagt cgtcacctgg cgtggcgtgg ctgtacagta agacgctatg ctggcgccgc
     5341 cggtcttccc acgcgacgac agtgcctttc ctcgtcatga taaccgtcgg atatgactga
     5401 tcgtccactg gtggtttggc atccaccggt tttatcgtac ctgcctgcgc tactttgcca
     5461 tcagcgccga tttgcagttc ctgcgccata acgcggttga attttcctgc ccgttgacac
     5521 cagactgcca caacctgttt cccatcctgc gtcgccaagg caatctgcgt ggcgtccttg
     5581 ccgctcagct tcagcgcggg ccccaggcca tgtggcgaca cctgacgcac ccagacagca
     5641 ttatcctgct cccagccggc gagaaaccga ttgttaggca gcgccagaat ggccggaccg
     5701 aaagccgact tgccggagct gattcgctgt gctgtggaga attcattttt gtccgtggcc
     5761 ttgaaaacaa tatgggcctg gggcgagcca ttgcggttat cttcccacac aacagcgaga
     5821 gtatttccag acatggccat acttttgcgt ccggtggcgt ccaggtgctg aaacaccctg
     5881 gaatcactga ctggcgcaac ggggattttt tcgccaaagt cccagcccgc ggcgcgggcc
     5941 agcgcactga tgaacagcat cagaacaaag caatacttac atctcatcgg gtctgcgcga
     6001 ggaactgatc gagggtctga ttcttgtggt ggccgtcgtg aatgtactgg tcgtacttga
     6061 tgaagtcctg caccgtctgg aaaccgggaa ccttgagcag ggatttcccc tcggcatcca
     6121 tataaatgaa gaccggtgtt accttggcct tgaggcgcgc gcccagttcc atttccgtga
     6181 tgcgttcgcc gtttggcagc accaggcgct ttccgctttc ggcgtccaca taggtgagga
     6241 cgtagtgttc ggtatagagt ttcttcagcg cggggtcact gaaggatttt ttgttggtct
     6301 tgtcgcagaa accgcagccg tagcggccgt agtagacgaa gattttttta ttctgctgcc
     6361 gggccgactt gagggcctcg tcgtatcgca ggaacggata acccggcggc gcatcggcgt
     6421 ggctgacatt ggaaaagacc gctgcccaag ctgccagcat aatgcacggc cacaaaagtc
     6481 tccgcataat cgcccctagt gctttaacct actggcttgc ttagttattt gaagctgttg
     6541 agaatattac gtcatataaa tgtatgacgc actacccagg cgatttcatt ccggacttgt
     6601 ctaactcaat gttcaggcga gtaaaagctc ataccagatc ttcagggcgg tggcggcgat
     6661 cagcaccgac aaggccccgc gcaacagccg gcggggggtt ttctggctca tgacgctgcc
     6721 caattgcgcc ccgatcagca ccccggccac cagcactgcc gacatcagat aaggtatctg
     6781 gttggttccc aggcggccga cgaggaccgc cgccga
//
LOCUS       DBNGZE010000330         2829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1083896, whole genome shotgun sequence.
ACCESSION   DBNGZE010000330 DBNGZE010000000
VERSION     DBNGZE010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1083896"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="AC16FV_08575"
     CDS             <1..1483
                     /locus_tag="AC16FV_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR2850146.1"
                     /translation="GTWQGRNETGELVASADLRGLKLALPAPLDRPDGLADEKLVVRT
                     ESARRDNMVLAFNVGSGMNGKLALARDGGNWKFAGARIDFGGSAPAPVPKTRDVYIRA
                     DVATVDADQWWPLLGGGPTEVPDFLVRISASVKTLNMFDRQFENIVTDFSRSRDVWQG
                     TVNGAAMAGSVKYSGKGPTARFEMDLAHLVLPDAQYERGEASVDPRRLPAVQLRSKSF
                     ELRHKQLGELDFMAEPVKSGWRIERFNLARADMKLNAGGSWEFDGKNHESQFDIEFSS
                     PDMGKTMEAFDAPDRLAGGEVSVKSHLSWSGSPTNPHLAALSGKVNVSAKKGRFLQVK
                     AGAGRLFGLLDLSAIGRYLTLDFSPVFGKGLLYDRIQGEVNIEKGNAYTSGFSIRGPA
                     TQLDVGGRVGLVAEDFDLALELQPKISDTVTIATWGVLGPQVAAAVLAVQKIFKKQIA
                     KGTRITYVVKGPWENPTITKLVKGDVADVPKEPGKADDAADVR"
     gene            1606..2418
                     /locus_tag="AC16FV_08580"
     CDS             1606..2418
                     /locus_tag="AC16FV_08580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR2850147.1"
                     /translation="MTLVAAVQMVSGPRVPDNFEEAGRQIAAAAAAGAQLVALPENFA
                     LMPLNDADRLAAAEQDGKGPIQDFLAAQARQHQVWLVGGTIPLAAKTGNKVRAACLVL
                     NDRGERVARYDKIHLFDVQLDNGEKYHESNSFEPGEKVVTVDTPYGKLGLAVCYDLRF
                     PELFRRLQEQGAEIFAVPSAFTAHTGKAHWEVLVRARAIENLVYVIAAAQGGRHANGR
                     ETHGDSMIVNPWGEVLARLAHGAGHVIAECDHARLQDVRKNLPSIRHRRLHI"
     gene            2415..>2829
                     /locus_tag="AC16FV_08585"
     CDS             2415..>2829
                     /locus_tag="AC16FV_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PmbA/TldA family metallopeptidase"
                     /protein_id="HIR2850148.1"
                     /translation="MSEGMLTLARKSLLEPAGLGETELSRLMGQLMSGSVDYGDLYFQ
                     YSRHESWSLEEGKVKSGSHSIEQGVGVRAQAGEKTGFAYSDEILLPALTRASDMARAI
                     ARQGGQNGLKAWTPNAPQRLYAPIDPLPTLADADKV"
BASE COUNT          527 a          840 c          956 g          506 t
ORIGIN      
        1 gggtacttgg cagggcagga acgaaaccgg cgagctcgtg gcctcggccg atctgcgcgg
       61 cctcaagctg gcgttgcccg cgccgctgga ccggcctgat ggtctggcgg acgagaaact
      121 ggtggtccgc accgaatccg cgcgtcgcga caacatggtg ctggcgttca atgtcggcag
      181 cggcatgaat ggcaaactgg ccctggcgcg cgatggcggg aactggaaat tcgccggcgc
      241 gcgtattgat tttggtggca gcgcgccggc gccggtcccc aaaacccgtg atgtgtatat
      301 cagggccgat gtggcgacag tcgatgccga tcaatggtgg ccgctgttgg gcggcggacc
      361 gaccgaagtt ccggatttcc tcgtgcgcat cagcgccagc gtgaagacgc tgaacatgtt
      421 cgatcgtcag tttgaaaata tcgtcactga tttttcccgc agtcgtgacg tctggcaggg
      481 cacggtcaat ggcgcggcca tggccggttc cgtgaagtat tccggcaagg gccccacggc
      541 acgcttcgaa atggacctgg cgcatctggt gctgccggac gcgcagtacg agcgcggcga
      601 ggcgtcggtg gacccgcgcc gcctgcccgc cgtgcaattg cgtagcaaat ccttcgagct
      661 tcgccacaag caactgggcg agctggattt catggccgag cccgtgaaat ccggctggcg
      721 catcgagcgg ttcaatctgg cgcgcgccga catgaaattg aacgccggcg ggagctggga
      781 attcgacggc aagaaccatg agagccagtt cgatatcgag ttcagcagcc cggacatggg
      841 caagaccatg gaggcgttcg acgcgccgga tcggctggcg ggcggcgagg tgtcggtcaa
      901 gtcgcacttg tcgtggtcgg gttcgccgac caatccgcat ctggcggccc tcagcggcaa
      961 ggttaatgtg tccgcgaaga agggccgctt ccttcaggtg aaggcgggcg ccggccggct
     1021 gttcggcctg ctcgacctga gcgccatcgg ccgctacctc acgctcgatt tcagcccggt
     1081 gttcggcaag ggcctgctct acgaccgcat ccagggcgag gtgaacatcg aaaaaggcaa
     1141 tgcctacacc agcggttttt ccattcgcgg gccggcgacc cagctcgatg tcggcggccg
     1201 ggtcggattg gtggcggaag atttcgatct ggcgctggaa ttgcaaccaa aaatcagcga
     1261 caccgtcacg atcgcgacct ggggcgtgct ggggccgcag gtggcggcgg cggtgctggc
     1321 ggtgcaaaag attttcaaga aacagatcgc caagggtacc cgcatcactt atgtggtgaa
     1381 ggggccatgg gagaatccga ccatcaccaa gctggtcaag ggtgacgtcg ccgacgtgcc
     1441 gaaggaaccg gggaaagcgg atgatgcggc cgatgttcgg tgatattcta gcggcatgct
     1501 gcggagtttg attttccctc tccagtcgtt gtttttcctg acatccatcg cggaatttgt
     1561 ttgctcactt gctgagcgtg tgtgcgatcc gcgcgcggtg acgccatgac gctcgtggcc
     1621 gcggtacaga tggtgtccgg gccgcgagtg ccggataatt tcgaggaagc ggggcggcag
     1681 atcgccgccg cggccgctgc cggcgcgcaa ctcgtggcgc tgccggaaaa tttcgcgctc
     1741 atgccgctca acgatgccga tcgtctggcg gcggcggagc aggacggaaa aggccccatc
     1801 caggattttc tggcggcgca ggcgcgccag catcaggtgt ggctggtggg cggcaccatt
     1861 ccgcttgccg caaagacagg caataaagta cgcgcggcct gcctggtgtt aaatgaccgg
     1921 ggagaacgcg tcgcgcgtta cgacaagatt catttattcg acgtacagct ggacaatggc
     1981 gagaaatatc atgagtcgaa cagcttcgaa ccgggcgaga aggttgtcac ggtcgatacc
     2041 ccgtatggaa aactggggct cgccgtgtgc tacgacctgc gtttccccga gctcttccgc
     2101 cgcttgcagg agcagggcgc ggagattttc gccgtgcctt ccgccttcac cgcgcacacc
     2161 ggcaaggccc actgggaggt gctggtgcga gcgcgtgcca ttgaaaatct ggtttacgtc
     2221 atcgccgcgg cgcagggtgg ccgtcacgcc aacgggcgcg aaacccacgg tgacagcatg
     2281 atcgtgaatc cctggggcga ggtactggcg cggcttgccc acggcgcggg tcatgtcatc
     2341 gccgagtgcg atcatgcgcg tttgcaggac gtgcgcaaga atctgccgag catccggcac
     2401 cggaggctac acatatgagc gaaggcatgc tgacactggc ccggaagtcg ctgctggaac
     2461 ccgccggcct gggtgaaacg gaactttcac gtctcatggg ccagttgatg tcgggcagcg
     2521 tggattacgg cgatctctat tttcaataca gccggcacga atcctggtcg ctggaagaag
     2581 gcaaggtcaa atccggcagc cacagcatcg agcagggcgt gggcgtgcgc gcccaggcgg
     2641 gcgagaagac cggcttcgcc tattccgacg agatcctgct gccagccctc acccgtgcct
     2701 ccgacatggc ccgggccatc gcgcgccagg gcggccagaa cgggctcaag gcctggacgc
     2761 ccaacgcgcc ccagcgcctg tacgcgccca tcgatccgct gccgaccctc gccgacgccg
     2821 acaaggtga
//
LOCUS       DBNGZE010000331         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_325464, whole genome shotgun sequence.
ACCESSION   DBNGZE010000331 DBNGZE010000000
VERSION     DBNGZE010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_325464"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="ntrC"
                     /locus_tag="AC16FV_08590"
     CDS             complement(<1..1066)
                     /gene="ntrC"
                     /locus_tag="AC16FV_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR2850149.1"
                     /translation="MAKQDIVWVIDDYRSIRWVLEKALTQADMAVKTFDSANEIADLL
                     ERQRPDVVISDIRMPGISGLELLKTIHAKAPEIPVIIITAHTDLESAVASYRGGAFEY
                     LPKPFDVDEAVRLTRRAVEHRRRQKVESPTNDQTPEIIGSAASMQEVFRAIGRLSGSH
                     LNVLINGESGTGKELVARALHNHSPRADKVFIAINIAAIPSELLESELFGHEKGAFTG
                     ATQSRKGRFEQANEGTLFLDEIGDMPPGLQTRLLRVLQDGKFYRVGGHEQVETNVRII
                     AATNQNLEIRVKEGKFREDLFHRLNVIRIHLPPLRERREDIPTLAGYFLKKSAVELGV
                     ETKQLAPAAEEFLGRLPWPGN"
     gene            complement(1059..>1896)
                     /gene="glnL"
                     /locus_tag="AC16FV_08595"
     CDS             complement(1059..>1896)
                     /gene="glnL"
                     /locus_tag="AC16FV_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR2850150.1"
                     /translation="RGVPLKLPGNRTITVDCTVTPLTDVGPDGALLVELTQVDRLLRL
                     ARDESMLERQAANRAVMRGLAHEIKNPLGGLRGAAQLLERELPDKSLREYTRIIIHEA
                     DRLRNLVDRMMGPHKPLKKQPVNIHEILEHVRKLMLVEVPVGLTLERDYDPSLPEFEG
                     EPEQLIQAVLNIVRNSAEAIQHKGTIRLSTGIERSFTIGQKRHRLVLRADIEDNGPGI
                     PEEIQEHIFYPMVTAKPGGTGLGLSIAQDIIAKHGGLIECRSRPGHTVFSIYLPLENG
                     HG"
BASE COUNT          292 a          605 c          619 g          380 t
ORIGIN      
        1 cgttgcccgg ccagggcagc cggccgagga attcctccgc cgccggcgcc aattgcttcg
       61 tctccacgcc gagctcgacg gcggacttct tcaggaagta gccggccagc gtcgggatgt
      121 cctcgcgccg ctcgcgcagc ggcggcaggt ggatgcggat cacgttcagc cgatggaaca
      181 ggtcctcgcg gaatttccct tccttcaccc ggatttccag attctgattc gttgcggcga
      241 tgatgcgcac gttggtctcc acctgttcgt gcccgccgac gcggtagaac ttgccgtcct
      301 gcagcacgcg tagcagccgt gtttgcaggc ccggcggcat gtcgccgatc tcgtccagaa
      361 acagcgtgcc ttcattggcc tgctcgaagc ggcccttgcg gctctgggtg gcgccggtga
      421 aggcgccttt ctcgtggccg aacaattcgg attcgagcaa ctcgctcggg atcgcggcga
      481 tgttgatcgc gataaaaacc ttgtcggcgc gcgggctgtg attgtgcagg gcgcgcgcca
      541 ccagttcctt gccggtgccg gattcgccgt taatgagcac gttcaagtgc gatcccgaca
      601 atcgcccgat ggcgcgaaac acctcctgca tcgaagccgc cgagccgatg atctccggcg
      661 tctgatcgtt ggtcggcgat tccacttttt gccgccgccg gtgttcgacg gcgcggcgcg
      721 tgagacgcac cgcctcgtcc acgtcgaacg gcttgggcag gtactcgaac gcgccgccgc
      781 gataggaggc gaccgcgctt tcgaggtcgg tgtgggcggt gatgataatg accggaatct
      841 ccggcgcctt ggcgtgaatg gtcttgagca attcgagccc gctgatgccg ggcatacgga
      901 tgtcggagat caccacgtcc ggacgctggc gttcgagcag gtcggcgatt tcattggccg
      961 agtcgaaggt cttcacggcc atgtccgcct gcgtcagggc cttttccagc acccagcgga
     1021 tggagcggta gtcgtcgatg acccaaacaa tatcctgctt agccatgccc gttctccagc
     1081 ggcaggtaaa tgctgaacac agtatgcccc gggcggctgc ggcactcgat caggccgcca
     1141 tgcttggcta tgatgtcctg cgcgatggaa aggccgaggc cggtgccgcc gggcttggcc
     1201 gtgaccatcg gatagaaaat atgctcctgg atttcctcgg ggatgcccgg gccgttgtcc
     1261 tcgatgtcgg cgcgcagcac caggcggtgg cgcttctggc caatggtgaa gctgcgttcg
     1321 atgccggtgc tcaggcggat cgtgcccttg tgctggatcg cctcggccga gttgcgcacg
     1381 atgttgagca ccgcctgaat cagctgttcg ggttcgccct cgaactccgg caggctcggg
     1441 tcgtagtcgc gttcgagcgt gaggccgacc ggcacctcca ccagcatgag cttgcgcacg
     1501 tgttccagaa tttcatgaat attgacgggc tgcttcttca gcggcttgtg cggccccatc
     1561 atgcggtcca ccaggttacg cagacggtcg gcctcatgga tgatgatgcg ggtgtattcc
     1621 cggagcgact tgtccggcag ttcgcgttcc agcagctgcg ccgcgccgcg caggccgccg
     1681 agcgggttct tgatctcgtg cgccaagccg cgcatcaccg cgcggttggc cgcctgacgc
     1741 tccagcatgc tttcgtcgcg tgccaggcgc agcagccggt ccacctgcgt gagctcaacc
     1801 agcaaggccc cgtccggacc cacgtcggtc agcggtgtga ccgtgcaatc cacggtaatg
     1861 gtgcggttgc ccggcagctt cagcggcacg ccgcgg
//
LOCUS       DBNGZE010000332        12083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_811201, whole genome shotgun sequence.
ACCESSION   DBNGZE010000332 DBNGZE010000000
VERSION     DBNGZE010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_811201"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="AC16FV_08600"
     CDS             complement(<1..897)
                     /locus_tag="AC16FV_08600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR2850151.1"
                     /translation="MKKESQIFLLESQLRDREFEIAMLRETAEAISGELDLEKVFQVV
                     ADHARKLILAETVLIPVLDQKLAQYTYRAGCGKNADEIVGQSLPLDIGVCGWVWRHKR
                     PWWHGVLDELEEEERNRWEKEAGSIILVPLIGKRHFLGGIAGINKIGGKDFTQRDLDL
                     LTLFAHEVSFAVENAELFDQLNKSKEQAEEYQRDLQALNAELERRVAQRTTALADAVK
                     ELEHLALHDMLTDLPNRSLVEDRLQQGIHIAKREKKVISLMTIDLNRFKEVNDKHGHD
                     VGDSLLRQVALRLRRVLRQSDTA"
     gene            complement(1046..2989)
                     /locus_tag="AC16FV_08605"
     CDS             complement(1046..2989)
                     /locus_tag="AC16FV_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR2850152.1"
                     /translation="MQRYFAFVLFLVVLLGVARTAFALDITQEEENRRRDYLTALEAL
                     KAGQHARFKTLYNSLDGYILRGYLEYESLKNRVSSASAAEIRRFLADNDQAPISDVIR
                     KKWLRVLASRGDWPTFLKEYQDIEDEPELQCLRLSHLLKTSEQQATLMAEAEQLWLTG
                     KQLPRSCDPVFSAWKQAGHMTADKVWARIRLAMEARNLRLASELAVHLDPSERIWVKR
                     WEAMHRDPVRELHNIAYPVETPVARMVVKHGVVRLAYRDPEEAMIQWHLLKGKYQFFG
                     EDDNYVMRNIGILAAHDQLPQALVWLSEVSADPADETLHLWRVKAALRAGQWETAKRF
                     IAGLSEEQQHESRWRYWSARILEATGSKSEARSLYQGLARDRSYYGFLAADRVDADYS
                     MQHVGVEASPEEIGAMLARPGIQMAQELYHMGQVNDARRQWNWTMRRMNNRELAVAAV
                     IAQQWGWHDRAILTVAKSDQQDDLELRFPLLYRDVIEANASEAGIDPSWVYGVVRQES
                     AFVVDARSAAGALGLMQLMPSTGRLTGRKINMPIRNNRALLNVENNLRLGASYLKEVL
                     GRNNNNQVLATASYNAGPNRVSSWLPQRNMEADIWVELIPFGETRDYVKNVMSYTTVY
                     DHRLGSRPARLRNRMMDIAPPEK"
     gene            3059..4417
                     /locus_tag="AC16FV_08610"
     CDS             3059..4417
                     /locus_tag="AC16FV_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR2850153.1"
                     /translation="METINPATGERLKTFDTWNEAQVDSALAVAAAANPAWQATPFVE
                     RARLFRNIATELRHNTAHYAGIITLEMGKIVKEARAEIEKCAWGCEFYAENAEIFLRD
                     EVIQTDASSSYVSYLPLGTVLAVMPWNFPFWQVFRFAAPSLMAGNTALLKHASNVPQC
                     ALAIEEIFRKAGFPKGVFQTLMISAAQAEKIIADPRIHAVTLTGSEAAGRKVAAAAGA
                     TLKKTVLELGGSDAFIVLADADLEQAVNVGIASRFLNCGQSCIAAKRFILVETIAEAF
                     VEQFKHKVHALRLGDPTREETQIGPMARADLRDQLHKQVTDSVTAGTTFALHGGPAAG
                     PGFFYEPTVLDHVRPGMRAYEEELFGPVAVVIRARDEEEAVRIANDNRYGLGATVFTR
                     DVKKGERLARRIQSGSSFVNGMVKSDPRLPFGGIKASGYGRELSVHGIREFVNIKTMW
                     VK"
     gene            complement(4476..5057)
                     /locus_tag="AC16FV_08615"
     CDS             complement(4476..5057)
                     /locus_tag="AC16FV_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2850154.1"
                     /translation="MRMQYIARAAIASVAMTLSIAANAASSDDWYAGINLGQSENKTR
                     VAKIAGTGFTGSLDNEDSSWKVYGGYRLWDQYVAVEVSYADLGKTTASGSTSSGTQEI
                     KAFTIGVLGYIPIVDPFGAIIHLGFSRNDSRTETVTGSTGTFSGATDFEFYWGAGLQY
                     DFAKNFGIRAEIERFSIDGADVNLRTAGVYYRF"
     gene            complement(5253..6038)
                     /gene="lgt"
                     /locus_tag="AC16FV_08620"
     CDS             complement(5253..6038)
                     /gene="lgt"
                     /locus_tag="AC16FV_08620"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR2850155.1"
                     /translation="MLAPDIDPVAIHLGPLKVHWYGLMYLIGFLGAWWLGVYRAKRPA
                     SGWQPQEIGDLLFYGALGVILGGRLGYVLFYNLAYYLEHPAEVFYIWSGGMSFHGGLL
                     GVITAMWLYARRTRRGFFAVADFVALLTPLGLGAGRLGNFINHELWGRVTDVPWGMVF
                     PNAGSLPRHPSQLYEFMLEGVALFVILWVYARKPRPTASVSGLFLVCYGTFRFMVEFV
                     REPDAQLGYLAFGWLTMGQVLSLPMVLFGIGLIWWAHHKKTVP"
     gene            complement(6083..6991)
                     /locus_tag="AC16FV_08625"
     CDS             complement(6083..6991)
                     /locus_tag="AC16FV_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2850156.1"
                     /translation="MPETRLAIKVDVDTDRGTRVGVPWLLDILDEFGVKATFLFSLGP
                     DNTGRAIRRIFRPGFFGKAARTNVVAIYGLRTLLNGVLWPGPHVGRRNQDVMRAARDR
                     GHEVGIHCYDHIRWQDGLARMSRAEVHEEFDKACREFERVFGEPAQTAGAAGWQANAL
                     SLAAYDDANLLYASDARGTDAFFPLAGGVAFRTLQIPTTLPTLDELLGRPEHPEDRLV
                     EQYLSWLRPGRLNVLTAHAEIEGMLKVDLFRALLAQAQKKNVSFVRMDDLAREFLRQR
                     ERIPVCELIQGEVEGRSGTLAVQKAA"
     gene            complement(7001..8041)
                     /locus_tag="AC16FV_08630"
     CDS             complement(7001..8041)
                     /locus_tag="AC16FV_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HIR2850157.1"
                     /translation="MSLKVLILGVNGFIGNSLTERILRDTDWEVYGMDMGRDKLDQCL
                     GHERFHYVEGDITINKEWIEYHVKKCDVVLPLVAIATPATYVLEPLRVFELDFEANLA
                     IVRQCVRYGKRVIFPSTSEVYGMSTDREFDEEASNLVLGPINKPRWIYSCSKQLLDRV
                     IAAYGQQHGFKYTLFRPFNWIGPKLDNIMEPKEGSSRVLTQFIGNILRGKDIQLVDGG
                     AQRRSFTYLDDGVDALLRIIANKDGCADGRIFNIGNPNTAMSVRELAETLVALVKTYP
                     KYAALAEKTKLITVSSGDYYGKGYQDILTRVPSIRNAEKYLGWKPTTDLKTALRKTLD
                     YHLGRPSRELEA"
     gene            complement(8054..9106)
                     /locus_tag="AC16FV_08635"
     CDS             complement(8054..9106)
                     /locus_tag="AC16FV_08635"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HIR2850158.1"
                     /translation="MTEPGIVVFGYHDVGYECLDVLIARGSRILAVFTHHDDPGENIW
                     FKSVAELARRHGIPVHAPDSVNTPEWIARLREMKPDIIFSFYYRNMICQEIVDIPRLG
                     AFNMHGSLLPKYRGLVPINWAVLNGETQTGATLHWMVRRPDAGDIVDQEAVSIGPHDT
                     ARDVFIKVTAAARKILERNVDAIKQGRAARRPQDESQASYFGGRKAEDGRIDWRADAR
                     QIFNLVRAVTHPYPGAFTDVQDAQMPRAAALDVPVSRGISASMPVTGRAGAAEVDGRR
                     FFIWWAEPITDCAGMPGEVISVSPLRVATGKGCLEIRQWQWRGGLEQEGATHGLRLNQ
                     VLGEAAPGQHRFNSPI"
     gene            complement(9099..10040)
                     /locus_tag="AC16FV_08640"
     CDS             complement(9099..10040)
                     /locus_tag="AC16FV_08640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850159.1"
                     /translation="MAKPYVTVVIPVYNEQENLPALFLRLTTALDKFGQPYEILFTND
                     GSRDNSLVLLRDFHRQRPKQVRVIDFNGNFGQHMAIMAAFERARGDVIVTLDADLQNP
                     PEEIPKLLREIEKGHDVVGGYRKDRHDSWFRRNASRLLNVIRERTTRIRMRDQGCMLR
                     AYRRHIVEHISASQETSTFIPALAMSFAANPAEVEVEHAARAAGESKYGLYKLIRLNF
                     DLMTGFSVVPLQMFTLFGMVLAAGSLVFVIYLFIRRLMIGPEAEGVFTLFAILYFLVG
                     VGIMGLGVIGEYIGRIYQEVRRRPRFVIREVYEQLND"
     gene            complement(10042..11199)
                     /locus_tag="AC16FV_08645"
     CDS             complement(10042..11199)
                     /locus_tag="AC16FV_08645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR2850160.1"
                     /translation="MTEEFLPFSRPSISRAAIDEVVACLESGWITTGPRVKKFEDELK
                     RYFNAPHALALSSATAGLHLVLAALELKPGDEVITTPMTFAATLNTIVLAGGRPVLVD
                     VEPDTYNMDVSRLAKAITKRTRAIMPVHFAGLPVDLDPLYELARKHGLRVIEDAAHAI
                     GTEYKGKRIGAFGDTQVFSFHPNKNMTTGEGGCVVTRDDKLAEKIALLRFHGMDREAW
                     NRFGKAGSQHYEIITPGFKYNMMDIQAALGLHQLPALDGFIKRRTELALRYQKILADW
                     PQWTRPGTPKFIHRHAWHLYTPLINPAAAGMDRDAFMQGMKERNIGTGLHYRAMHLYP
                     YYRENYGFKPGDFPNAERIGECIVSLPLFPGMRDADQDRAIAAMADLFKRK"
     gene            complement(11210..11575)
                     /locus_tag="AC16FV_08650"
     CDS             complement(11210..11575)
                     /locus_tag="AC16FV_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR2850161.1"
                     /translation="MNYFPLILLGVLLNAGAQLLLKEGMRRVGYFEFAWANVVPIGWQ
                     VAANPFVLAGIFAYVVSVLVWLLVLSRVEVSFAYPMLSVGYIVNAVAGYYLFQENLSM
                     TRITGILIIIAGVYLVTRS"
     gene            complement(11572..>12083)
                     /locus_tag="AC16FV_08655"
     CDS             complement(11572..>12083)
                     /locus_tag="AC16FV_08655"
                     /inference="COORDINATES: protein motif:HMM:NF037277.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850162.1"
                     /translation="PNLYFMAGSLLLAAVVPFVFSRRRNCRHTIVSLLVVSELLIVSF
                     DVSLPRLNEYRSVKPLALTLKPILRHDDEVMSYQTYYQDLPVYLERRITVVGWKGELE
                     FGTQVEDTSGWIIDEPAFWQRWQGARRVYLLTALDNYDKLLAENRGRFYVIAQSGSNM
                     LLTNRTIQP"
BASE COUNT         2393 a         3716 c         3661 g         2313 t
ORIGIN      
        1 ggcggtgtcc gactgccgca gcacgcgccg caggcgcagg gccacctgcc tcaacaggct
       61 gtcgcccacg tcgtgcccat gtttgtcatt gacctccttg aaccggttga ggtcgatcgt
      121 catgagcgag atgactttct tctcgcgctt cgcgatatgg attccctgtt gcaggcggtc
      181 ctcaaccagc gaacggttcg gcaggtccgt cagcatgtca tgcagcgcta ggtgctcgag
      241 ctccttcacg gcgtcggcca gggcggtggt gcgctgggcg acgcgccgct ccagttcggc
      301 gttgagggcc tgcagatcgc gctggtattc ctcggcctgc tccttggatt tgttcagctg
      361 atcgaacagc tcggcatttt caaccgcgaa gctcacctca tgggcaaaga gtgtaagcag
      421 atcgagatcg cgctgggtga agtccttgcc gccgatcttg ttgatgccgg caatgccgcc
      481 gagaaaatga cgcttaccga tgagcggaac gagaatgatg ctgcccgcct ctttctccca
      541 ccggtttctt tcctcctctt ccagctcgtc gagcacgccg tgccaccagg ggcgcttgtg
      601 gcgccacacc cagccgcaga caccgatatc cagcggcagg gactggccca cgatctcatc
      661 ggcgttcttc ccgcatccgg cgcggtaggt gtattgggcg agtttctgat ccagcaccgg
      721 gatcaagacg gtctcggcga ggatgagttt gcgcgcgtgg tcggcgacca cctgaaatac
      781 tttttcgagg tcgagttcgc cgctgatggc ctcggcggtt tcgcgcagca tggcgatctc
      841 gaattcgcga tcgcgcagct ggctctccag cagaaagatt tggctttcct ttttcaaaat
      901 gcccctaccc tgggccgccc ggcttgccgg ggcggattag taatgtacga cctgtaggga
      961 tgagtttaga tcaggaaaat gccggccgta ccacatggcc ggtggcggaa aatcaggctt
     1021 ttaccggggt gtccgggaag cggggttatt tttccggcgg cgcgatgtcc atcatgcggt
     1081 tgcgcaggcg cgccgggcgg ctacccagac gatggtcgta caccgtggtg taggacatga
     1141 cgttcttcac gtagtcgcgc gtctcgccga acggaatcag ctcgacccag atatcggcct
     1201 ccatgttgcg ctgcggcagc cagctgctga cgcggttggg gccggcgttg tacgaggccg
     1261 tggccagcac ctggttattg ttattgcggc ccagcacctc cttcagatag ctggcgccga
     1321 ggcgcaggtt gttctcgacg ttcaacagtg cgcggttgtt gcggatcggc atgttgatct
     1381 tgcggcccgt gagtcggccg gtggagggca tcagctgcat caatccgagc gcgccggccg
     1441 cggagcgggc atcaacgacg aaggcggatt cctggcgcac cacgccgtaa acccagctgg
     1501 gatcgatgcc ggcctcggag gcgttggcct cgatcacgtc gcgatacagc aacgggaagc
     1561 gcagctcgag atcgtcctgc tgatcgctct tcgccaccgt caggatggcg cggtcgtgcc
     1621 agccccactg ttgcgcgatc accgccgcca ccgccagctc gcgattgttc atgcgccgca
     1681 tggtccagtt ccattgacgc cgcgcgtcgt tcacctgacc catatgatac agctcctgcg
     1741 ccatctggat gcccggacgc gccagcatgg cgccgatctc ctccgggctg gcctcgacgc
     1801 cgacgtgctg catcgaatag tccgcgtcga cacgatccgc ggcgaggaag ccgtaatagc
     1861 tgcggtcacg cgccaggccc tgatacaggc tgcgcgcctc gctcttgctg cccgtggcct
     1921 cgagaatgcg cgcgctccag tagcgccagc ggctctcgtg ctgctgttcc tcgctcaggc
     1981 ccgcgatgaa gcgcttcgcc gtctcccatt gtccggcacg cagcgccgct ttcacgcgcc
     2041 acaggtggag cgtttcgtcc gcggggtcgg cggaaacctc cgacagccat accagcgctt
     2101 ggggcaactg gtcatgcgcc gccagaatgc caatgttgcg catgacataa ttgtcgtcct
     2161 cgccgaaaaa ctggtacttg cccttcagca gatgccactg aatcatcgcc tcctcggggt
     2221 cgcggtaggc gaggcgcacg accccatgtt tgaccaccat gcgcgccacc ggcgtctcca
     2281 ccggataggc gatgttgtgc agctcgcgca ccggatcgcg gtgcatcgcc tcccaccgtt
     2341 tgacccatat gcgttcggat gggtcgagat ggacagcgag ctcactggcg agtcgcaagt
     2401 tgcgcgcttc catggccaga cggatgcgcg cccagacctt gtcggcggtc atgtggccgg
     2461 cctgcttcca ggcactgaat accggatcgc aggaccgtgg caattgtttg cccgtgagcc
     2521 agagctgttc ggcctcggcc atgagcgtcg cctgttgttc gctggtcttg agcaggtgcg
     2581 acaggcgcag gcattgcagt tccggctcgt cctcgatgtc ctggtattcc ttgaggaagg
     2641 tgggccaatc gccgcggctg gcgagcacgc gcagccattt cttgcgaatg acgtcgctga
     2701 tcggcgcctg gtcgttgtcc gcaaggaacc gccggatttc ggcggcggac gcggatgaca
     2761 cacggttttt cagggattcg tattcgagat aaccacgcag gatatatccg tccaggctgt
     2821 tataaagcgt cttgaagcgg gcgtgttgcc ccgccttcag agcctccagc gcggtcagat
     2881 agtcgcggcg gcggttttcc tcttcctggg taatatccag cgcaaacgcg gtcctggcca
     2941 cgcccaacag aacgaccaga aacagaacaa aagcaaagta ccgttgcatg ctgtgtttat
     3001 tatagataac aacacctaaa ataggcggcg ctgaacggta acaatgctct caacagtcat
     3061 ggaaacgatc aatcccgcca ccggcgaacg gctgaaaacc ttcgacacat ggaatgaggc
     3121 gcaggtggat tccgcgcttg ccgttgcggc agcggccaat ccggcctggc aggcaacgcc
     3181 gttcgtcgag cgcgcgcgac tgtttcgtaa tatcgccaca gaattgcgcc ataacaccgc
     3241 tcattatgcc ggaattatca cccttgagat gggcaagatc gtcaaggagg cgcgcgccga
     3301 gatcgagaaa tgcgcctggg gttgcgagtt ctatgccgag aacgccgaga tctttttgcg
     3361 cgacgaagtc atccagaccg acgcgagttc cagttacgtc agctatctgc cgcttggcac
     3421 ggtgctcgcg gtcatgccat ggaattttcc cttctggcag gtgtttcgct tcgcagcgcc
     3481 gagcctgatg gcgggcaata ccgcgctgct gaaacacgcc tcgaacgtcc cgcaatgcgc
     3541 gctggcgatc gaggagattt tccgcaaggc cggttttccc aagggggttt ttcagacgct
     3601 catgatctcc gccgcgcagg cggaaaagat catcgccgac ccccgcatcc acgccgtaac
     3661 gctgaccggc agcgaagcgg ccggacgcaa ggtcgcggcg gccgccggtg cgacgctcaa
     3721 gaaaaccgtg ctggaactcg gcggatcgga tgccttcatt gtgctggcgg acgcggacct
     3781 ggaacaggcg gtgaatgtcg gcattgcctc gcgctttttg aattgcggcc agagctgcat
     3841 cgcagcaaaa cggtttattc tggtagaaac gattgccgaa gccttcgtcg agcaattcaa
     3901 gcacaaagtc catgcgctac gcctgggcga cccgacccgc gaagaaaccc agatcggtcc
     3961 gatggcgcgc gctgacctgc gcgatcagtt gcacaaacag gtgaccgatt ccgtcacggc
     4021 gggcaccacc ttcgcgctgc acggggggcc cgcagcgggc ccgggatttt tctacgaacc
     4081 gacggtgctc gaccatgtcc ggccggggat gcgcgcctat gaggaggaat tgttcggccc
     4141 ggtggccgtt gtcatccgcg ccagggacga ggaagaggcg gtccgcatcg ccaacgacaa
     4201 ccgttacgga ctgggcgcga cggtattcac gcgcgacgtg aaaaaaggcg agcgactggc
     4261 acggcgcatc cagtccggtt caagcttcgt caacggcatg gtcaagagtg acccgcgcct
     4321 gcccttcggc ggcatcaagg cctcgggcta cggacgcgag ctgtccgtgc acggcatccg
     4381 cgagttcgtg aacatcaaga cgatgtgggt taaataacca aaaaaaacgg ccggggcatc
     4441 ccggccgttt gcgcaaacac atctgtcggt taccgtcaga aacgatagta gacacccgcg
     4501 gtgcgcaggt tgacatcggc accgtctata ctgaaacgct ctatttccgc gcgtattccg
     4561 aagtttttgg caaaatcgta ttgcaggccg gcgccccaat aaaattcaaa gtcggtcgcc
     4621 ccggagaacg taccggtgct gcctgtgacg gtctcggttc tcgaatcgtt gcgggagaac
     4681 ccgaggtgga taatggcacc gaacggatca acgatcggaa tataaccaag gactccaatc
     4741 gtaaacgcct tgatttcctg cgtaccggat gaggtcgaac cgctcgcggt ggtcttgcct
     4801 aaatcggcat aggacacctc aactgcaaca tactggtccc acaggcgata gcccccgtaa
     4861 actttccaac ttgaatcttc attgtccagg ctgccggtga agccggtccc ggcaattttc
     4921 gcgactctgg ttttgttctc ggattgtccc aaattaatac cggcatacca gtcatctgaa
     4981 ctggcggcat tggccgcaat ggacaacgtc atcgccacag atgcgatagc agcccttgca
     5041 atatattgca tacgcatgat tctcttcctc ctccagaata tatttcaaag cgaattgtta
     5101 agatttgcgc cagccccacc tcttcatggg tgaagacagg aaggcggaca tcttgttatt
     5161 gcgggcaaac aataccgcct gtcccccggt aacgtcaatc ggcggcaact aactgaactt
     5221 ctaaggcagt catccaaaac aggcggcggg cattatggca ccgtcttttt atggtgggcc
     5281 caccatatca gtccgatccc gaacagcacc atcggcagac tcaacacctg ccccatcgtc
     5341 agccagccga aggcgagata acccagctgg gcgtcaggtt cacgtacaaa ttccaccata
     5401 aaacggaacg ttccgtagca aacaagaaac aggccggaaa cggaagctgt cgggcgtggc
     5461 ttgcgcgcgt aaacccacag gatgacgaaa agcgcgacac cttccagcat gaattcataa
     5521 agctgcgagg ggtggcgcgg cagtgatccg gcattgggaa acaccatacc ccagggaaca
     5581 tcggtgacgc gaccccagag ttcgtggttg atgaaattgc cgaggcgtcc cgcgcccagc
     5641 ccgagcggcg tcaacagcgc gacaaaatcg gcgacggcaa aaaacccgcg ccgggtcctg
     5701 cgcgcataca accacatggc ggtgattacg ccgagcaggc cgccgtggaa cgacatgccg
     5761 ccggaccaga tgtagaacac ctccgccgga tgttcgaggt aataggcgag gttgtagaaa
     5821 agaacataac ccagtcgtcc gccgagaatg acgccgagcg cgccgtaaaa gaggagatcg
     5881 ccgatctcct gcggctgcca gccggaggcc ggacgtttcg cccggtacac gcccagccac
     5941 caggcgccga ggaaaccgat gagatacatc agtccatacc agtgaacctt gagcgggccg
     6001 agatgaatgg cgacgggatc gatgtcgggg gcaagcatgg cgcgggatta tgaacgagaa
     6061 ggattgctgc cgccagacaa gttcatgccg ctttctgcac cgcgagcgtg ccggaacggc
     6121 cctctacctc accctggatc agttcgcaga caggaatgcg ttcgcgctgg cgcagaaatt
     6181 cacgcgccag atcgtccatc cgaacgaaac ttacgttctt tttttgtgcc tgcgcgagca
     6241 gggcgcgaaa caggtcaacc ttcagcattc cctcgatctc ggcgtgcgcg gtcagcacat
     6301 tgagccgtcc cggacgcagc cacgacaggt attgctcgac caagcgatct tcgggatgtt
     6361 ccggacgccc gagcaattcg tcgagcgtcg gcaacgtcgt cggaatctgc aaggtcctga
     6421 aggcaacgcc gccggccagc ggaaagaagg catccgttcc acgtgcgtca ctcgcgtaaa
     6481 gcaggttcgc atcatcataa gccgccaggc tcagggcatt cgcctgccag ccggcggcgc
     6541 cagcggtttg cgcgggctcg ccgaagacgc gctcgaactc gcggcacgcc ttgtcgaatt
     6601 cttcatgaac ctcggcgcgc gacatgcgcg cgaggccgtc ctgccagcgg atatggtcgt
     6661 aacaatggat tccgacttcg tggccgcggt cgcgcgcggc gcgcatcacg tcctggttgc
     6721 gcctgcctac gtgcgggccg ggccagagca cgccgttcag cagcgtgcgc aacccataga
     6781 ttgcgacgac gttggtgcgc gccgccttgc cgaaaaaccc gggacggaaa atgcgccgga
     6841 tggcgcggcc ggtattgtcg ggaccgagcg agaaaagaaa agtcgccttg acgccgaatt
     6901 catccaggat atccagcagc cacggcacgc cgacccgcgt gccgcgatcg gtgtccacat
     6961 cgaccttgat cgcgaggcgc gtctccggca tggccgtggt tcaggcttcc agttcccgcg
     7021 acggccggcc gaggtggtaa tcgagcgtct tgcgcagcgc cgtcttgagg tcggtggtcg
     7081 gtttccagcc aagatatttt tcggcgttcc tgatcgacgg cacgcgcgtg agaatgtcct
     7141 ggtagccctt gccgtagtaa tcgcccgagc tgaccgtgat gagtttggtc ttttccgcga
     7201 gcgccgcgta cttgggatag gtcttgacca gcgccaccag cgtctcggcc agctcgcgca
     7261 ccgacatggc ggtgttcggg ttgccgatgt tgaagatgcg cccgtcggcg catccatcct
     7321 tgttggcgat gatgcgcagc agcgcgtcca cgccgtcgtc gagataggtg aaactgcgcc
     7381 gctgcgcgcc gccgtcgacc agctggatgt ccttgccgcg caagatgttg ccgatgaact
     7441 gggtcagcac gcgcgagctg ccttccttgg gctccatgat gttgtcgagc ttcggtccga
     7501 tccagttgaa cggacgaaac agggtgtact tgaagccgtg ctgctggccg taggcggcga
     7561 tcacgcggtc gagcagctgc ttgctgcacg aatagatcca gcgcggcttg ttgatcggcc
     7621 cgagcaccag gttcgacgct tcctcgtcga attcacggtc ggtgctcatg ccgtagacct
     7681 cggaggtcga ggggaaaatc acgcgtttcc cgtagcgcac gcactggcgc acgatcgcga
     7741 ggttggcctc gaagtcgagc tcgaacacgc gcagcggctc gagcacgtag gtcgcgggcg
     7801 tggcgatggc cacgagcggc agcaccacgt cgcatttctt gacgtgatac tcgatccact
     7861 ccttgttgat ggtgatgtcg ccctcgacgt agtggaagcg ctcgtggccg aggcactggt
     7921 cgagcttgtc ccggcccatg tccatgccgt agacctccca gtcggtgtcg cgcaggatgc
     7981 gctcggtcag gctgttgccg atgaaaccgt tcacaccgag gatcaaaacc ttgagactca
     8041 tactgactcc attttatatg ggtgagttaa accgatgctg ccccggagcg gcttcaccca
     8101 gaacctggtt aagccgcaga ccatgtgtcg cgccttcctg ttccagaccg ccgcgccact
     8161 gccattgcct gatttcaaga cagccctttc ccgtcgccac ccgcaagggc gagaccgaaa
     8221 tgacctcgcc cggcatgccc gcacaatcag ttatgggctc ggcccaccag atgaaaaacc
     8281 gccgaccatc cacctccgcc gctcctgcgc gtcctgtgac gggcatggaa gcgctaatgc
     8341 cgcgagagac agggacgtcg agagcggccg cccgcggcat ttgtgcatcc tgcacatcag
     8401 tgaaggcgcc gggataggga tgggtcacgg cgcgtacgag attaaaaatt tgccgggcat
     8461 cggcacgcca atcgatccga ccatcctcgg ctttgcgccc gccgaagtag cttgcctgcg
     8521 actcgtcctg tggacggcgg gcggcgcggc cctgcttgat ggcgtccacg ttccgttcca
     8581 ggattttccg tgccgccgcc gtcaccttaa taaagacatc ccgcgccgtg tcgtgcgggc
     8641 ctatggacac cgcttcctga tccacgatat cgccggcatc cggccgcctg accatccagt
     8701 gcaaggtggc gccggtttga gtttcgccgt tcagcaccgc ccagttgatc ggcacgagcc
     8761 cgcgatactt cggcaacagc gagccgtgca tgttgaaagc gccgagacgc ggaatatcca
     8821 caatttcctg acaaatcatg ttgcggtaat aaaacgaaaa aatgatatcc ggtttcattt
     8881 cacgcaggcg cgcgatccac tccggcgtgt tcaccgaatc cggcgcatgt accggaatcc
     8941 cgtgccggcg cgccagttcc gcgaccgact tgaaccagat gttctccccg ggatcgtcgt
     9001 ggtgcgtgaa caccgcaagg atgcggctgc cgcgcgcaat caggacgtcg agacactcat
     9061 agcccacgtc atggtaaccg aaaacaacga tgccgggctc agtcattaag ctgctcgtaa
     9121 acctcgcgga tcacgaaacg cggacggcgg cgaacctcct gataaatgcg gccgatgtac
     9181 tcgccaatga cgcctagccc catgatgccc acgcccacca agaaataaag gatcgcgaac
     9241 agggtgaaca cgccttccgc ctccggaccg atcataaggc ggcggatgaa cagataaatc
     9301 acgaacacca ggctgccggc ggccagcacc atgccgaaca gggtgaacat ttgcagcggc
     9361 acgacggaaa accccgtcat caggtcgaaa ttgagccgga tcagcttgta caggccgtac
     9421 ttcgattcgc cggccgcgcg cgcggcgtgc tccacctcca cctcggcggg attggcggca
     9481 aaactcatgg ccagcgccgg gataaacgtg gaggtctcct ggctggcgct aatgtgttcg
     9541 acgatgtgac gccggtaggc gcgcaacatg cagccctggt cgcgcatgcg gatgcgcgtg
     9601 gtgcgttcgc ggataacgtt gagcaggcgc gaggcgttgc ggcgaaacca ggaatcgtgg
     9661 cgatccttgc ggtagccgcc gacgacgtca tgccccttct cgatttccct caagagcttc
     9721 gggatttctt ccggcggatt ctgcagatcg gcatcgaggg tcacgatcac gtcgccgcgc
     9781 gcgcgctcga acgccgccat gatcgccatg tgctggccga agttgccgtt gaaatcgatc
     9841 acgcgcacct gtttcggccg ctgcctgtga aaatcgcgca acaacaccag cgaattgtca
     9901 cggctgccgt cgttggtgaa caggatttcg tagggctggc cgaatttgtc gagcgccgtt
     9961 gtcaggcgca ggaacagggc cggcaggttt tcctgctcgt tgtacacggg aatgacgacg
    10021 gtgacgtaag gcttcgccac gttatttcct cttgaacaaa tccgccatgg cggcgatcgc
    10081 ccggtcctgg tcggcgtcgc gcatgccggg gaacagcggt aggctcacga tgcactcgcc
    10141 gatgcgttcg gcgttgggga aatcgccggg cttgaagccg tagttctcac gataataagg
    10201 atacaggtgc atcgcgcggt aatgcaggcc ggtgccgata ttgcgctcct tcatgccctg
    10261 catgaaggcg tcgcggtcca tgcccgcggc cgcgggattg atcagcggcg tgtacaggtg
    10321 ccaggcgtgg cgatggatga atttcggcgt gccgggccgg gtccattgtg gccagtctgc
    10381 gaggattttc tggtagcgca gcgccagctc cgtgcgccgt ttgatgaaac cgtcgagcgc
    10441 cggcagctgg tgcaggccaa gcgcggcctg gatatccatc atgttgtact tgaagcccgg
    10501 agtgattatt tcgtagtgct gggacccggc tttgccgaaa cggttccagg cctcgcggtc
    10561 catgccgtga aaccgcagca gcgcgatctt ctccgccagt ttgtcgtcgc gcgtcaccac
    10621 gcaaccgccc tcgccggtgg tcatgttctt gttcggatga aaactgaaca cctgggtgtc
    10681 gccgaaggcg ccgatgcgct tgcctttgta ctcggtgccg atggcgtgcg ccgcgtcctc
    10741 gatcacacgc agcccgtgct tgcgcgccag ctcgtacagc ggatcgaggt ccaccggcag
    10801 tcccgcgaaa tgcaccggca tgattgcgcg ggtgcgcttc gtgatcgcct tcgccagtcg
    10861 tgaaacatcc atgttatagg tgtcgggctc gacgtccacc agcaccggtc ttcccccggc
    10921 cagcacgatg gtgttgagcg tagcggcgaa ggtcatgggc gtggtgatca cctcgtcgcc
    10981 gggtttcagt tccagcgccg cgagcacgag gtgcaagccg gcggtggcgg aagacaacgc
    11041 gagcgcgtgc ggcgcgttga aatagcgctt cagctcgtcc tcgaattttt ttacccgcgg
    11101 gccggtcgtg atccagccgg attcgaggca ggccaccacc tcgtcgatcg ccgcgcggct
    11161 gatcgagggc cgcgagaacg gaaggaattc ctcggtcatg gctccgtgcc taactgcggg
    11221 tgaccagata aacgccggcg atgatgatga gaataccggt gatgcgcgtc atggaaaggt
    11281 tttcctggaa caggtaatag ccggccacgg cattgacgat gtaacccacg gacaacatgg
    11341 ggtaagcgaa gctcacctcc acgcgcgaca gcaccagcag ccacaccagc acgctcacga
    11401 cataggcaaa aatgcccgcc aacacgaacg gattggccgc cacttgccag ccgatgggaa
    11461 ccacgttggc ccaggcgaac tcgaaataac cgacgcgccg catgccttcc ttgagcagca
    11521 actgggcgcc ggcattcagc agcacgccca gcaaaatcag cggaaaataa ttcacggttg
    11581 aatcgtcctg ttggtcagaa gcatgtttga cccgctttgc gcgatcacat aaaaccggcc
    11641 gcggttctcg gcgagcagtt tgtcatagtt atccagggcg gtcagcaggt acacccggcg
    11701 cgccccttgc cagcgttgcc agaaggccgg ttcgtcgata atccagccgc tcgtatcctc
    11761 aacctgcgtg ccgaattcga gctctccctt ccatcccacc accgtgatcc ggcgctccag
    11821 atacaccggc aggtcctgat aataggtctg ataggacatg acctcatcgt cgtgacgcag
    11881 aatcggttta agcgtcaggg ccagcggttt gaccgagcgg tactcgttca ggcgcggcag
    11941 gcttacgtcg aacgaaacaa taagcagctc cgacaccacc agcagggaaa caatggtgtg
    12001 gcgacaattg cgccggcggc tgaagacgaa gggaaccacg gccgccagca acagactgcc
    12061 agccatgaaa tacagattcg gcc
//
LOCUS       DBNGZE010000333         1946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1644908, whole genome shotgun sequence.
ACCESSION   DBNGZE010000333 DBNGZE010000000
VERSION     DBNGZE010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1644908"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="AC16FV_08660"
     CDS             <1..883
                     /locus_tag="AC16FV_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR2850163.1"
                     /translation="GTLIDESNIEQIVVIGYDYVGISIDGLHATHDRFRRLEGAYDAS
                     MTAIRLCHQKGSKVGMRFTRTQDNARELPELLNLMEAEGIDKFYLSHLNYAGRGNINR
                     KDDVILKTTRWAMDLLFDRCWDYTQRGLHKEFVTGNNDADGVYLLFWVRKNFPHLEEH
                     LRAKLAQWGGNSSGVNIANIDNLGNVHPDTFWWHYSLGNVKERPFSQIWHDISDPLMA
                     GLKASPRSIKGRCGVCQYFDICGGNTRVRAHQLTNDPWAEDPACYLTDEEIGVENTGE
                     RLVNRPYTRIPIARASK"
     gene            893..>1946
                     /locus_tag="AC16FV_08665"
     CDS             893..>1946
                     /locus_tag="AC16FV_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR2850164.1"
                     /translation="MMQACRRSAFFLCVSFAFSAASVLAAEAPSVVKLYEEYCVSCHG
                     ADRFGKMGPALLPENLGRLKREEAQAVIRDGRALTQMPPFREKLSDEQIKGLADFIYT
                     PPVQTPRWDLADIRASQVIHHKPGSLPDKPVFKADPLNLFVVVETGDHHVTILDGDKF
                     EPVQRIPTRFALHGGPKFSPDGRFVYFGSRDGWVSKFDIYNLKYVAEIRAGINMRNIA
                     VSSDGRYVLAGNYLPHTLVLLDANDLTPLKIIPVRDDAGKSSRVSAVYDAAPRKSFIA
                     ALKDIAEVWEIPYDDKAEPVYPGLVHDYRMGEALAEKGPFPVRRIRLDDYLDDFFFDQ
                     SYENLIGAARNGKNGQV"
BASE COUNT          406 a          603 c          561 g          376 t
ORIGIN      
        1 cggcacgctg atcgacgaat ccaacatcga gcagattgtc gtgatcggtt acgactacgt
       61 cggcatcagc atcgatggcc tgcacgcaac ccatgaccgt ttccgccgtc tcgaaggcgc
      121 gtacgacgcc tccatgacgg cgattcggct atgtcatcaa aagggcagca aggtcggaat
      181 gcgcttcacc aggactcagg ataacgcccg cgagcttccc gagctgctga atctcatgga
      241 ggccgaaggc atcgacaagt tctatctgtc gcatctcaac tatgccgggc gcggcaacat
      301 caaccgcaag gatgacgtga tcctgaaaac cacgcgctgg gccatggacc tgctgttcga
      361 ccgctgctgg gactatacgc agcgcggttt gcacaaggaa ttcgtcaccg gcaacaacga
      421 cgccgatggc gtttatctgt tgttctgggt gcggaaaaac ttcccgcatc tcgaagaaca
      481 cctgcgcgct aagctggcgc agtggggcgg caactcctct ggcgtgaaca tcgcgaatat
      541 cgacaatctc ggcaatgtcc accccgacac cttctggtgg cattacagtc tgggtaatgt
      601 aaaggaacgg ccgttctcgc agatctggca tgatatctct gatccgctca tggccgggct
      661 caaggccagt ccacggagca tcaagggccg ctgcggtgtc tgccaatatt tcgatatatg
      721 cggcggcaac acgcgcgtgc gcgcacacca gctcaccaac gatccctggg ccgaggatcc
      781 agcctgctat ctcaccgacg aggaaatcgg cgttgagaac acgggcgaac gtcttgtgaa
      841 ccggccctac acgcgcattc ccatcgcccg cgccagcaaa tagacttgcc acatgatgca
      901 agcttgtcgg cgttccgctt tcttcctctg tgtgtctttt gcgttctccg cggccagtgt
      961 cctggccgcg gaagcgccct ccgtagtaaa actttacgaa gaatactgcg tttcctgtca
     1021 cggggccgat cgcttcggaa aaatggggcc ggcgctgttg cccgagaacc tagggaggtt
     1081 gaagcgcgag gaggcgcagg ccgtcatcag ggatggccgc gcgttgacgc aaatgccgcc
     1141 attccgcgaa aagctctcgg atgagcagat caagggcctc gcggatttca tctacacgcc
     1201 gccggtacag acgccacgtt gggacctggc ggacatccgt gcctcgcagg tcatccacca
     1261 caagcccggc agtctgccgg acaagccggt tttcaaggcc gatccactga acctgtttgt
     1321 cgtcgtggaa acgggtgacc atcatgtcac cattctggat ggcgacaagt ttgagccggt
     1381 ccagcgcatc ccgacacgct tcgcgctgca cgggggtccg aaattctcgc cagacgggcg
     1441 cttcgtctat ttcggctcgc gcgacggttg ggtgagcaag ttcgacatct ataacctcaa
     1501 gtacgtcgcc gaaatccgcg ccggcatcaa catgcgcaac atcgccgtct ccagcgacgg
     1561 ccgatacgtg ctggcgggca attatctgcc gcacacgctg gtgctgcttg acgccaacga
     1621 tctcacgcca ctcaagatca tcccggtgcg ggatgacgcc ggcaagagct cgcgcgtgag
     1681 cgccgtctac gacgccgcgc cgcgcaagag cttcattgcc gcactcaagg acatcgccga
     1741 agtgtgggag attccctacg acgacaaggc cgaaccggtc tatccggggc tggtgcacga
     1801 ttaccgcatg ggcgaggcgc tcgccgagaa aggacccttt ccggtgcggc gcatccggct
     1861 cgatgactat ctcgatgact ttttcttcga tcagtcttat gaaaatctca tcggcgccgc
     1921 gcgcaacggc aagaacggcc aggtgg
//
LOCUS       DBNGZE010000334         2630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1367175, whole genome shotgun sequence.
ACCESSION   DBNGZE010000334 DBNGZE010000000
VERSION     DBNGZE010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1367175"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16FV_08670"
     CDS             <1..417
                     /locus_tag="AC16FV_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850165.1"
                     /translation="SAAPKTAVRKKTASKKAVVKKKAAAKPKAAPKVKRAAASKPAAM
                     PKPVAPAAPPGMERIGVVTHYYNHLSVAIVQLETGTLRVGDFIHIRGHTSDFSQPVES
                     LEIDHVHVNEARPGQSFGLRVKEHAREHDVVYKAKP"
     gene            complement(513..788)
                     /locus_tag="AC16FV_08675"
     CDS             complement(513..788)
                     /locus_tag="AC16FV_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR2850166.1"
                     /translation="MSCLLAGLLAWLATSPAEARRDGHSPGHERASRERLSLDEAVVN
                     VRRDTGGRVLSAQTREERNGTSYRIKVLLPNASVRSFDVEGKDRGRR"
     gene            complement(806..1258)
                     /locus_tag="AC16FV_08680"
     CDS             complement(806..1258)
                     /locus_tag="AC16FV_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850167.1"
                     /translation="MNRKLLSAAVLAVVASLSAPLVLADRGDRDDYRDRDSYREVRVH
                     QVEERYSRHDNGLHRGWCKDKHKYGHDGRRDYYERREYREYSPRGDHGDDGYYEERRT
                     SSDAPIILGSVIGGVVGHEIGRGDPGHTAVGAVIGTVIGYDIARHRDY"
     gene            1427..2308
                     /locus_tag="AC16FV_08685"
     CDS             1427..2308
                     /locus_tag="AC16FV_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR2850168.1"
                     /translation="MGIAKHPSVVTLPAAQEPYPSIVEFLCRAFPAISRERWAQRMRE
                     GKVLNDRGEPITTDTPYLPSKRIFYFREIENEPVIPFAEKILFQDGELLVACKPHFLP
                     VIPGGRYVEECLLNRLRARTGIADLAPLHRLDRETAGIVIFSVNPKTRGLYHGLFMHG
                     KAEKIYHALAEVNQPPREDRWTVENRIVRGEPRFRMKIVAGAVNARSHIQLLEVKHNR
                     GLFRLQPVTGKTHQLRLHMSGLGFGIMNDRVYPDLQPERDDDFDRPLQLLAREIRFHD
                     PVTGAEREFRSERELLW"
BASE COUNT          583 a          773 c          754 g          520 t
ORIGIN      
        1 tccgcggcac ccaaaaccgc ggtccgcaaa aaaactgcct ccaaaaaggc tgtagtcaaa
       61 aagaaagccg cggcaaaacc caaagccgcg cccaaggtaa agcgcgcggc ggccagcaag
      121 ccggccgcga tgccgaaacc ggtcgccccg gcggcgcctc ccggcatgga gcgcatcggt
      181 gtggtcacgc attactacaa tcatctctcg gtcgcgatcg tgcaactcga aaccggcacc
      241 ctgcgcgtgg gtgacttcat tcacatcagg ggacacacca gcgatttctc acagccggtc
      301 gagtcgctgg agatcgacca cgtgcacgtg aacgaggcgc ggcccggcca gtcctttgga
      361 ttgcgcgtca aggaacacgc gcgcgagcac gacgtcgttt acaaggccaa gccctgattc
      421 acgtcatgca caaacactgg cggaaaatca aaagaggatt ttgcgggacc ttggccgtca
      481 aggcagagtt gtttgtataa ggaaacggga agtcaacgac gcccgcgatc tttcccctcg
      541 acgtcgaaag aacgcaccga cgcattcggc aataaaacct tgatccggta agaggtgccg
      601 ttgcgctctt cgcgcgtctg cgccgaaagc acgcgcccgc cggtatcgcg ccggacattc
      661 acgaccgcct cgtcaagcga aagcctttcc cgggatgctc tctcatggcc tggcgaatgg
      721 ccgtcgcgtc gcgcctccgc cggggacgtg gcgagccacg ccagcaatcc ggccaggagg
      781 catgacatga atttcatgtt ttgcatcaat agtcacgatg acgcgcaatg tcgtagccga
      841 tgacggtgcc gatcaccgca ccgaccgccg tgtggccggg atcgccacga ccgatttcat
      901 gtccgaccac gccgccgatc acgctcccga gtataatggg cgcgtccgag ctggtgcgac
      961 gctcttcgta ataaccatca tcgccatggt ctccgcgcgg gctgtattcg cggtattcac
     1021 ggcgctcata gtaatcgcga cggccgtcat gtccgtactt gtgtttgtcc ttgcaccatc
     1081 cgcggtgcag accgttatca tggcgcgaat aacgctcttc cacctgatgc acccggacct
     1141 cgcggtaact gtcgcggtca cggtaatcgt cacgatcgcc gcgatccgcc agcaccagcg
     1201 gcgcggacag cgaggcaacc acggccagga cggcggcact cagtaacttg cggttcatgt
     1261 ttatctcctt cgtttgtgtt taagggttac cgcttacttc agggataagg ttacgcgccc
     1321 atggctgaac gctgactgaa cggaattcag ttatatcttg ttgattaacc tggctttaaa
     1381 attcacaccg gcgaaaccaa tacggcttct ggagatagat aaacaagtgg gcatagccaa
     1441 acacccatcc gtcgtgaccc tgccggcagc acaagagccc tatccttcga tagtggaatt
     1501 tctgtgccgg gcattcccgg ccatctcgcg cgagcggtgg gcgcaacgca tgcgcgaagg
     1561 caaggtattg aacgaccggg gcgagcccat caccacggac acgccgtatc ttccctccaa
     1621 acgtattttc tatttccggg aaatcgaaaa cgaaccggtt attccattcg cggaaaaaat
     1681 cctgtttcag gatggcgagt tgctggtggc ctgcaagccg cattttctgc ccgtgattcc
     1741 gggcggccgt tatgtggagg aatgcctatt gaaccgtctg cgcgcccgca ccggcattgc
     1801 cgatctggca ccgctgcacc ggctcgaccg cgagaccgcg ggcattgtca ttttttcggt
     1861 caatccaaaa acccgcggtc tttaccatgg gttgttcatg cacggaaagg cggaaaagat
     1921 ttatcacgcg ctggcggaag tgaatcagcc gccgcgggaa gaccggtgga ccgtcgaaaa
     1981 ccggattgtc cgcggcgaac cgcggttccg gatgaaaatc gtcgcggggg ccgttaacgc
     2041 ccggtcgcat atccagctgc tggaagtgaa acacaaccgg gggcttttcc gtttgcaacc
     2101 ggtgaccggc aaaacccatc aattgcggtt gcacatgagc ggtttggggt tcgggatcat
     2161 gaatgaccgg gtttatcccg atctgcaacc ggaacgagat gatgatttcg accggccgct
     2221 ccaactgctg gccagggaga tccgattcca tgaccctgtc acgggtgccg agagggagtt
     2281 tcgatcggag cgagagctat tgtggtaatg ccatagatta agggtacgag atgattgtga
     2341 gagtcccgct ccgaccttcc tggaaattat tcctcactgc aaaccgcgtt ctatccccta
     2401 ttactctcgc gttcccgcga actgatggca tacagattga cgcgcatcaa agcagtcggc
     2461 gatttgccgt ggctagatgt ttgttagatt gtcgtgaatg gcacgagcca ggccattcaa
     2521 atgcgcgcat gacgcgtggg aggtgagcca tgaccggacg aaggaagcga gaagactaca
     2581 gtcatcagcc gagaagggat cgcatggatg cagagcgagt gagcgatccc
//
LOCUS       DBNGZE010000335         4414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_116874, whole genome shotgun sequence.
ACCESSION   DBNGZE010000335 DBNGZE010000000
VERSION     DBNGZE010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_116874"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="mdh"
                     /locus_tag="AC16FV_08690"
     CDS             <1..848
                     /gene="mdh"
                     /locus_tag="AC16FV_08690"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR2850169.1"
                     /translation="VVLIDIKEGVPQGTALDIQESAPIFDFDTKLSGSNDLQSMAGAD
                     MVVITAGIPRKPGMSRSDVLDTNLAVVHGIVDEVLKIAPKAMLLKVTNPVDVLTYAAW
                     KRSGFPRARVFGMAGVLDSARMASFVAMETGFSVKDVTAMVLGGHGDTMVPMTRFTCI
                     NGIPIEHFLSKEQIQKIVKRTREGGAEILSLRKTASAYDAPAAAIAAMVDAISHNRRH
                     ILPGVAVLQGEYGLHDICMGVPVVFGENGVEQVIELPLNAQEAAEFKRSAEMVRADLA
                     KLKNK"
     gene            845..1795
                     /gene="prmB"
                     /locus_tag="AC16FV_08695"
     CDS             845..1795
                     /gene="prmB"
                     /locus_tag="AC16FV_08695"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR2850170.1"
                     /translation="MTPPRRQARQESQISNSTTLTAADLIRLVERRFKRAKLYYGHGT
                     ETARDEAAWLVGHVLKIAPERLAGRLDKPVSPAALKKIDKLATARIRTRKPLAYLLKE
                     AWFAGRKFYVDERVIVPRSLTGEFITEQFQPWIDAKRVRRILDLCTGSGCMAIACAQA
                     FPKARVDAADISAEALAVARLNVKRQRMGRRVRLVRSNLFQALKGKHYDVIVTNPPYV
                     ARAEMKALPREYRHEPELALVSGRQGLDAIARILADAPRHLNPGGILVGEVGNSAEAL
                     QRTFPSVPFVWLTTSTGDECVFLLTAEELVRHRQAFKTSG"
     gene            complement(1792..3498)
                     /locus_tag="AC16FV_08700"
     CDS             complement(1792..3498)
                     /locus_tag="AC16FV_08700"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR2850171.1"
                     /translation="MNLTDQSNLSDLFVERVRETPDAPAYRQFDGSSWKDLTWGETAR
                     EVARWQAGFRKEGLKAGDRVALCLHNRVEWVLFDQAALGLGLVTVPLYFDDRPDNMAW
                     CLNDSGVRLLLLEDGKMWAALRDQAKTVERVVCLDAQATSDKKMISLQAWLPPVGANA
                     PARSGAAADDLATIVYTSGTTGRPKGVMLSHRNILSNITAAMQALPAHATDRFLSFLP
                     LSHMFERTCGYYSAIWAGAQTVYARSITLLADDIRGQRPTILISVPRIFERIYSKMQE
                     AMAPGSPKRRLFEKATEVGWRRFRGEATLGDRLLWPLLKLLVAKKIYRRLGGRIRMIV
                     VGGAAFAPQLARVFFGLGLPIIQGYGLTETAPVLAANRMGDNDPTSVGRALEGIELRC
                     DEKGELLARGPCVMLGYWNNPDATTAMIDQDGWLHTGDLAAIRGGNVYITGRVKDIIV
                     LSNGEKVPPTDAEQAILRDAVFEQAMVVGEGRPKLGLLAVSKNTDTKELCDRANAQLR
                     DFPGYARIHYLARVSDPWTVENGLLTPTLKLKRNEIEKRFTREIEAMYAGPDLCNSKN
                     AG"
     gene            complement(3528..>4414)
                     /locus_tag="AC16FV_08705"
     CDS             complement(3528..>4414)
                     /locus_tag="AC16FV_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2850172.1"
                     /translation="KTVNLNPAIGYKVNDQLSVGGGVNVQYIDATLTQAADFGKICAA
                     AGVGGLCGPAAGNDGHANVKADNTGYGYNLGLLWSPQMDTRLGFAYRSKINHKLEGSV
                     DMTVPSNVPASIIVAGGFVDSGAMAYITLPETISASVFHQINPTWDIMGDITRTRWSR
                     LPELRIDFDSAQPDSVVTLGLKDVNRYSAGANYRPGGAWSYRFGVALDRTPTPNESVR
                     TPRLPDEDRIWFAFGAGYRQSADLSFDMAFVHIKFDDASINKTAPPGDENQYRGNLMA
                     GYEADVNILSAQARWAFK"
BASE COUNT          868 a         1417 c         1318 g          811 t
ORIGIN      
        1 aggtggtgct gatcgacatc aaggaaggcg tgccgcaggg cacggcactc gacatccagg
       61 aatccgcgcc gatcttcgat ttcgacacca agctcagcgg ctcgaacgat ctccagtcca
      121 tggccggcgc cgacatggtg gtcatcaccg ccggcatccc gcgcaagccc ggcatgtcgc
      181 gctccgacgt gctcgacacc aatctggccg tggtccatgg catcgtcgac gaggtgctca
      241 agatcgcgcc gaaggcgatg ctgctcaagg tcaccaaccc ggtggacgtg ctcacctacg
      301 ccgcctggaa gcgttcgggt ttcccgcgcg cccgcgtctt cggcatggcc ggcgtgctcg
      361 actcggcacg catggccagc ttcgtggcca tggaaaccgg tttctcggtc aaggacgtca
      421 ccgccatggt gctcggcggc cacggcgaca ccatggtgcc gatgacgcgc ttcacctgca
      481 tcaacggcat tccgatcgag cacttcttgt cgaaggaaca gatccagaaa atcgtcaaac
      541 gcacgcgcga gggcggcgcc gaaattctca gcctgcgcaa gaccgcgagc gcctacgatg
      601 cccccgccgc tgccatcgcc gccatggtgg acgccatcag ccacaaccgc cgtcacatcc
      661 tgcccggtgt ggccgtgttg cagggtgagt atgggctgca cgacatctgc atgggcgtgc
      721 cggtggtgtt cggtgagaat ggcgtggaac aggtgatcga gctgccgctg aacgcgcagg
      781 aggccgccga gttcaagcgc tccgccgaga tggtccgcgc cgacctggca aagctgaaaa
      841 ataaatgacg ccgccaagac gccaagcacg ccaagaaagt caaatatcca attccacaac
      901 tctaaccgcg gcagatctga tccgcttggt tgaacgccgg ttcaaacgag cgaagcttta
      961 ctacggtcac ggtaccgaga ccgcgcggga cgaggccgct tggctggtcg gccatgtatt
     1021 gaaaatcgcg ccggagcggc tcgccggccg gctggacaaa ccggtctccc ccgcggcgct
     1081 gaaaaagatc gacaagctcg ccacggcgcg catcaggacg cgcaaaccac tggcgtacct
     1141 actcaaggag gcatggtttg cggggcgcaa gttctacgtg gacgaacgtg tgatcgtgcc
     1201 gcgttcgctc accggcgagt tcatcaccga acaattccag ccgtggatcg acgccaagcg
     1261 cgtgcggcgg attctggacc tctgcaccgg cagcggctgt atggccatcg cctgcgcgca
     1321 ggcttttcca aaagcgcgcg tggacgcggc cgacatttcc gctgaggcgc tggccgtggc
     1381 gcgccttaac gtcaaacgtc agcgtatggg ccggcgcgtc cggctggtgc gttccaatct
     1441 tttccaggcg ctgaaaggca agcactacga tgtcatcgtg accaacccgc cgtacgtggc
     1501 acgcgcggaa atgaaagccc tgccgcgcga ataccgccac gagccggaac tggcgctcgt
     1561 ttccggccgg caggggctgg atgccatcgc gcgcatcctg gcggacgccc cgcggcatct
     1621 caacccgggc ggaatactgg taggcgaagt cggcaacagc gcggaggcac tccagagaac
     1681 attcccctcg gtgccgttcg tgtggctgac cacgtccacg ggggacgagt gtgtatttct
     1741 gctgacggcg gaggaactcg tccggcaccg ccaagcattc aaaacttcgg gctaaccggc
     1801 attctttgaa ttacacaggt ccggaccggc gtacatggcc tcgatctcgc gcgtaaagcg
     1861 tttctcgatt tcattgcgct taagcttgag cgtgggtgta agcaagccgt tctccaccgt
     1921 ccacggatcg gaaacccgcg ccagatagtg aatgcgcgca tagccgggga aatcgcgcag
     1981 ctgggcattg gcgcgatcgc acagctcttt ggtgtcggtg tttttgctca ccgccagcag
     2041 tccgagcttg ggccggcctt cgccaacgac catcgcctgc tcgaacaccg catcgcgcag
     2101 gatcgcctgc tcggcatccg tcggcggcac cttctcgccg ttggacagca cgatgatgtc
     2161 cttgacgcgc ccggtaatat agacgttgcc gccgcgaatc gcggcgagat cgccggtgtg
     2221 cagccaaccg tcctggtcga tcatggcggt ggtcgcgtcc ggattgttcc agtaaccgag
     2281 catgacgcac ggcccgcgcg ccagcaattc gcctttttca tcgcaacgca gctcgatgcc
     2341 ttcgagtgcg cggcccacgg aagtgggatc attgtcgccc atgcggttgg ccgccagcac
     2401 cggcgcggtt tcggtgagcc cgtagccctg aatgatcggc agcccgaggc cgaagaacac
     2461 gcgcgcgagt tgcggggcga aggccgcgcc gccgacgacg atcatgcgga tgcgcccgcc
     2521 aagacgccgg taaatcttct tcgccaccag cagcttgagc agcggccaca gcagccggtc
     2581 gcccagcgtg gcctcgccac gaaaccgccg ccaaccgacc tccgtggctt tttcaaaaag
     2641 cctgcgcttg ggcgaaccgg gcgccatcgc ctcctgcatt ttggaataaa tgcgctcgaa
     2701 gatgcgcggc acgctgatga ggatggtggg ccgttgcccg cggatatcgt ccgccaataa
     2761 cgtgatcgag cgcgcataaa ccgtctgcgc cccggcccag atggcggaat agtaaccgca
     2821 ggtgcgctcg aacatgtgcg acaacggcag aaacgaaaga aaccggtcgg tggcgtgcgc
     2881 cgggagtgcc tgcatggcgg cggtgatgtt gctcaggata ttgcgatgcg acaacatcac
     2941 gcccttgggc cgtccggtgg tgccggaggt gtaaacgatg gtagccagat cgtcggcagc
     3001 ggcgccggaa cgcgccgggg cgttcgcccc caccggcggc agccacgctt gcaagctgat
     3061 cattttttta tcgctggttg cctgcgcatc gaggcacact acgcgctcga cggtcttcgc
     3121 ctgatcgcgc aacgcggccc acatcttgcc gtcttccagc agcaacaggc gcacgccgga
     3181 gtcgttcagg caccaggcca tgttgtccgg ccggtcgtcg aaatacagcg gcaccgtcac
     3241 caggcccagc cccagcgccg cctggtcgaa caggacccat tccacgcggt tgtgcaggca
     3301 cagggccacg cggtcgccgg ccttgaggcc ctctttcctg aaccccgcct gccaacgcgc
     3361 cacctcgcgc gccgtctcac cccaagtcag atccttccag ctgctgccat caaactggcg
     3421 ataagccggc gcgtccggtg tttcccggac gcgctcgaca aagaggtcgg agaggttgga
     3481 ctggtcagtc agattcatga cgcgctcctt ttcgccattg gacccaatta cttaaatgcc
     3541 cagcgcgcct gcgcgctcag aatgttgacg tcggcttcgt agccggccat gagattgccc
     3601 cgatactggt tttcgtcacc cggcggcgcc gttttgttaa tactcgcatc gtcaaatttg
     3661 atatgcacaa aagccatatc aaagctcaga tcggcgctct gtctgtaacc tgctccaaac
     3721 gcaaaccaga tgcgatcctc atccggaaga cgcggtgtgc gtactgattc attgggcgtg
     3781 ggtgtcctgt cgagcgctac cccgaagcga tagctccagg cgccgccggg gcgataattg
     3841 gcacctgccg aataacggtt gacgtccttc aggccaagtg tcaccactga atccggctgg
     3901 gcactatcga agtcgatgcg caactccggc aaccggctcc agcgagtgcg cgtgatatca
     3961 cccatgatgt cccatgttgg attaatctga tgaaacacac ttgccgaaat ggtttccggc
     4021 aatgtgatgt aagccatggc cccggaatcg acgaacccgc cagcgacaat tatcgaagcc
     4081 gggacattcg acggcaccgt catatcgacc gaaccttcaa gcttgtgatt aatcttggaa
     4141 cgataggcga acccaagccg cgtatccatt tgtggtgacc acaataaacc gaggttatag
     4201 ccatagcccg tattgtcggc cttgacgttt gcatggccgt cattgccagc cgcaggaccg
     4261 cacaatcctc ccacgcccgc ggcggcgcat atttttccga agtctgccgc ttgggtaagc
     4321 gtcgcatcaa tgtattgcac attgacaccg cccccaaccg atagctgatc gttgacctta
     4381 taaccgatgg ctggattcag atttacagtc ttta
//
LOCUS       DBNGZE010000336         2407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_811670, whole genome shotgun sequence.
ACCESSION   DBNGZE010000336 DBNGZE010000000
VERSION     DBNGZE010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_811670"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="AC16FV_08710"
     CDS             <1..787
                     /locus_tag="AC16FV_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR2850173.1"
                     /translation="DDLIEILRAAAERDLLDRDSLDMIEGVLQVSEMKVRDIMVPRSQ
                     MDVVDRNSPPEDYLPLVMESGHSRFPMVDGDKDKVVGILLAKDLLHYLFLDKKKRARF
                     NVHDMLRPAVFVPESKRLNVLLREFRTSRNHMAIVVNEYGSVAGLVTIEDVLEQIVGE
                     ISDEYDIDDDVMIMPRDGGEYVVKALVTLADFNSRLKTNLAHEEIETIGGLVMNRLGH
                     VPRRGEKVEIDNLRFEILRADSRRVYLLKVTPVEQPGRQKTRK"
     gene            753..2282
                     /gene="lnt"
                     /locus_tag="AC16FV_08715"
     CDS             753..2282
                     /gene="lnt"
                     /locus_tag="AC16FV_08715"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR2850174.1"
                     /translation="MNNQAGKKPGSNFFSLPPAWADTAAVAAGLLLPLAFSPFGLFPL
                     AIIAPALLFVLWRDISPGRAAWRGFLFGLGMFGLGVSWVYVSMHHYGNMPAPLAGFAV
                     FLFVAGLSLYPALLGWLQARLFPKTGSLHCIIVLPALWVLFEWTRGWFLTGFPWLNLG
                     YSQVATPLAGYAPWFGVYGVSFFCALSAGLLAAGIHTPERFLKRFLPLLTAIWVVGWV
                     AGKTEWVQSVNKPLQVTLIQGNIPLDSKWQSGNRRAIMDRYLALSTQSPRSDLIVWPE
                     SAIPAMLDQIDPAYLENLQRLGRATQTDFVLGVVERDRNDRRYYNSVISIGVSPGIYR
                     KQHLVPFGEYPPLDPLFRWLMRNLQIPMSDFSAGPPDQPPLMAAGQKIGVSVCYEDAF
                     GEEAIRALPQASLLVNVSEDAWFGDSFAPHQRLQMARMRAIEAGRPILRAANTGPSAV
                     IEHHGEVLARSPQFQTRALTATVQPMQGATPYVRFGNWPVISLLAFVAIAAVLGPRAR
                     K"
BASE COUNT          409 a          723 c          752 g          523 t
ORIGIN      
        1 cgacgacctg atcgagattc tgcgggccgc cgcggagcgc gacctgctcg accgcgattc
       61 gctcgacatg atcgagggcg tgctccaggt ctcggaaatg aaggtgcgcg acatcatggt
      121 gccgcgctcg cagatggacg tggtggacag gaacagtccg ccggaagatt acctgcccct
      181 ggtgatggag tccggccact cgcgctttcc catggtggac ggtgacaagg acaaggtggt
      241 tggtatcctg ctcgccaagg acctgctgca ttacctgttt ctcgacaaga agaaacgcgc
      301 gcgtttcaac gtgcacgaca tgctgcgtcc ggcggtgttc gtgccggaaa gcaagcgcct
      361 gaacgtgctg ctgcgcgagt ttcgcaccag ccgcaaccac atggcgatcg tggtcaacga
      421 atacggcagc gtggcgggcc tggtgaccat cgaggacgtg ctcgagcaga tcgtgggcga
      481 aatctcggac gaatacgaca tcgacgacga tgtcatgatc atgccgcgcg acggcggcga
      541 atacgtggtc aaggcgctcg tgacgctggc cgacttcaat tcccggctca agaccaatct
      601 tgcccatgag gaaatcgaga ccatcggcgg cctggtcatg aaccgcctcg gccatgtccc
      661 gcgccgcggc gagaaggtcg agatcgacaa cctgcgtttt gaaatcctgc gcgccgacag
      721 ccgccgtgtt tatcttctga aagtgactcc ggttgaacaa ccaggccggc aaaaaaccag
      781 gaagtaattt tttttcgctc ccgccggcgt gggctgacac ggccgccgtc gccgccggtc
      841 tgttgctgcc gttggcgttc tctcccttcg gtctgtttcc actggcgatc attgcccccg
      901 cgctgctgtt cgtgttgtgg cgcgatattt ctcccggccg ggccgcgtgg cgcggtttcc
      961 ttttcggtct cggcatgttc ggactgggcg tgtcatgggt ttacgtcagc atgcaccatt
     1021 acggcaacat gccggcgccg ctcgctggct tcgcggtttt tctgttcgtg gcggggctgt
     1081 cactttatcc tgcgctgctt ggatggttgc aggcgcgatt gtttccaaaa accgggtcat
     1141 tgcattgcat tattgtgctc cccgccctgt gggtgttgtt tgagtggaca cgtggctggt
     1201 tcctcaccgg tttcccctgg ctgaatctgg gttacagcca ggtcgccacg ccgctggccg
     1261 gttacgcgcc gtggttcggc gtgtacggcg tgagtttctt ctgcgcgctt agcgccggtt
     1321 tgttggcggc cggcatacac acgccggaaa gatttctaaa gcgttttttg ccgctgctca
     1381 ccgcaatctg ggttgttggg tgggtcgcgg gcaaaacgga gtgggtgcag tcggtgaaca
     1441 agccgctgca agtgacgttg atccagggca atatcccgct cgacagcaaa tggcagtcgg
     1501 gcaatcgccg cgccatcatg gaccgctacc tggcgctaag cacacaatcg ccccgctcgg
     1561 acctgatcgt ctggccggag tccgccatcc cggccatgct ggaccagatc gatcctgcct
     1621 atctggaaaa tttgcagcgt ctgggtcgcg cgacccagac ggatttcgtg cttggcgtgg
     1681 ttgagcgcga caggaatgat cgccgctatt acaacagcgt catcagcatt ggcgtgtctc
     1741 cgggaattta tcgcaaacag catctggtgc ctttcggcga atacccgccg ctcgatccgc
     1801 tgtttcgctg gctgatgcgg aatctgcaaa taccgatgtc ggatttcagc gccggcccgc
     1861 ccgaccagcc gccgctcatg gcggccggac agaaaatcgg cgtgtccgtg tgttacgaag
     1921 atgctttcgg cgaggaggcg attcgcgcgc tgccccaggc gtcgttgctg gtgaatgtca
     1981 gcgaggatgc ctggttcggc gattcattcg ccccgcatca acggttgcag atggcgcgca
     2041 tgcgcgcgat cgaggccgga cggccgatac tgcgtgccgc caacaccggc ccgtccgcgg
     2101 tcatcgaaca tcacggcgag gtgctggcgc gttcgccgca gttccagacg cgcgcgctca
     2161 cggccacggt gcagccgatg cagggggcca ccccctatgt gcgtttcgga aattggcccg
     2221 tcatatccct gttggccttc gttgcgattg cggccgtgtt gggtcctcgt gcccggaaat
     2281 agccggtcca acgcgtttat tgccgcatcc gtctggccgc ggatcacgcg cgggcatgtt
     2341 tcttgccgac ctttccggtt gatctattat aagttcataa ccagcacgaa cagaaagagc
     2401 cgtgccg
//
LOCUS       DBNGZE010000337         6185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_186282, whole genome shotgun sequence.
ACCESSION   DBNGZE010000337 DBNGZE010000000
VERSION     DBNGZE010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_186282"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16FV_08720"
     CDS             complement(<1..670)
                     /locus_tag="AC16FV_08720"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR2850175.1"
                     /translation="MSKIEKALSKARASGALRVVSPQPVPHENKPQETTSVHSDVTAL
                     IEQRASSSEAIARMQEKTPLSKQALAHNRVIYPELGENGTVRAFREIRTKIIQKSQGR
                     NCVIMVTSVTGGGGSSFVSLNLGVAFAFDAGKTALLLDCNLKNPSLDRLFGSGSFQGL
                     TDYMENSEMDITDIIHPVGIERLRIIPSGGQREIPAEYFTSLKMKRLLDSIKQRYRER
                     YVIID"
     gene            complement(672..2210)
                     /locus_tag="AC16FV_08725"
     CDS             complement(672..2210)
                     /locus_tag="AC16FV_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HIR2850176.1"
                     /translation="MNLRPDQIFKVLVNEAFFYRKAVVIGFFAINLLMLGLGLIWPKG
                     YTSSTTILVEDKKIIQPLMQGAAVATEVADRSRLAREVILGRKIMSQILEHAGWMKGN
                     PSPEEQEKIIEKLSRRTTITGAGKDLIKIEYKDDDPERAFRTTQKYAELFISESLGTK
                     IDESRAAFEFIDKQTQEYHDKLLKAEEQLKEFRSANLDARPGTDADISTRLNLLQTKI
                     EQSSQDLKEAEVKKLSLEKQLSGEAEVATALSREGQYRSRIAELQSRLETLRLSYHDN
                     YPDIIQIKHQIEDLNQAIVEERQHRETVKASGKVVIDEGVINNPMYQQLKQELSQTRV
                     NIEMLSARISEAKRQLQQELERGRRVHGGEATLAELTRDYQVNRDIYQDLLKRRENAR
                     VSMNLDKEKQGLTIKIQEPATLPLSPSGLRFLHFVIGGLVLGVLLPLGLLYAKLNLDP
                     RLRLAAMISEKHKVPLLAVVPHLWAPTETQAVRRELGWLSLAVNATLLIVVVTVALRV
                     AKVF"
     gene            complement(2227..2859)
                     /locus_tag="AC16FV_08730"
     CDS             complement(2227..2859)
                     /locus_tag="AC16FV_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HIR2850177.1"
                     /translation="MNLFRLVSVLLAGMFLVVSGCKSVPDATEGKEEVNRFASLLEGD
                     YRIGVDDRLQITVWRNPELSTTVPVRPDGKISVPLIGDVQAGGNTPTQVAAIIKDKLS
                     AYIREPNVAVILTELRSHEFLSRVRVTGAVRTPRSMPYRPGMTVLDAVLEAGGTNDFA
                     SPNRTKLYRKTKTKTEVFDVDLADILTKGKLESNFELKPGDVVTIPERLF"
     gene            3322..4227
                     /locus_tag="AC16FV_08735"
     CDS             3322..4227
                     /locus_tag="AC16FV_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR2850178.1"
                     /translation="MPDNYLKNYLILGVKPGASWKQLRQAYKKLVNVWHPDRFQQDIR
                     QKRLAEEKTKEITQAYKELAEYHKKFGVLPLPPEEEIPPARENNASPATPAKSTAPDT
                     VKTPEPASAPSGTKIPKHRMSKRRARALAAMALLGSIYFIWQFAPWERQDNAPANEGH
                     GDQSSNHHHETESVDVTPAEKRFTVGSSLGEVYAIQGVPTKTEKDIWYYGNSKVYFAK
                     GKVLRWEEKTDSPLRVDVISGFEKTTAGFFGRGSTKEEVLAVQGRPDRDAGSVWDYGL
                     SRVYFEKDRVTGWHEAQYNPLRVRN"
     gene            complement(4244..5644)
                     /locus_tag="AC16FV_08740"
     CDS             complement(4244..5644)
                     /locus_tag="AC16FV_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HIR2850179.1"
                     /translation="MIRLFKHYVPLPLFLMAITEAAVLFASMYAGTGIRFVWEDPGDI
                     NLIGPILPRALVFTAVMLAIMTALGLHQRDVRQEGEWGHLIRLMASFVVGLVTMLLIF
                     YAIPNLHLGRGAFGLVFLFAFVGTALTRLIFMRLFKLDAMHRRVLLLGAGSRSAKIEG
                     LERDENGERKFNLVGCLPLTNSDCCLDHTRVLHDKGPILSVARKYKIDEIIVGVRERR
                     GGGLPADQLLECKLAGIEVVDLPSFFERETGQIQVDSLNPSWMIFSDGFGRSPLKEVT
                     KRMFDVAASGVLLLLTLPVMLITASLIWLESGDPVFYRQERVGAHGRVFNVLKFRSMR
                     QDAERDGVPQWAKKQDDRVTRVGRIIRLLRIDELPQVFNVFRGDMSFVGPRPERPYFV
                     QDLSKKIAYYQSRHTVKPGITGWAQIRYPYGATVEDAVQKLQYDLYYVKNHTLFLDLI
                     ILFQTAQVIFFGKGAR"
BASE COUNT         1387 a         1825 c         1670 g         1303 t
ORIGIN      
        1 catcgatgat gacatagcgt tcgcggtaac gttgcttgat gctgtcgagg agccgcttca
       61 tcttgagcga tgtgaaatac tccgccggga tttcacgctg gccgcccgag ggaatgatgc
      121 gcaggcgctc gatgcccacc ggatggatga tatcggtgat atccatctcc gagttttcca
      181 tgtaatccgt cagtccctga aacgagcctg atccgaagag gcgatccagc gaggggttct
      241 tgagattgca atccagcaga agcgccgtct tgccggcatc gaaggcaaag gccacgccca
      301 ggttgagcga cacgaagctg cttccgccgc cgccggtcac cgacgtgacc ataatcacgc
      361 aattgcgtcc ctgggatttc tgaatgatct tggtgcggat ttcacggaag gcgcgcaccg
      421 tcccgttctc gccaagctcg gggtaaataa cgcgattatg ggccagcgcc tgcttgctga
      481 gcggcgtctt ttcctgcatg cgggcgatgg cctcgctgga actggcgcgc tgctctatca
      541 gcgccgtcac atcgctgtgt acggatgttg tctcttgcgg tttgttttcg tgcggcaccg
      601 gctggggcga caccactctc agggcgcccg atgcgcgggc cttgctcagg gctttctcaa
      661 tcttgctcat gtcagaacac cttggccaca cgcaatgcaa ctgtcaccac aacgataagc
      721 agggtcgcgt tcaccgccag cgacagccat cccagttcgc gacgcaccgc ctgtgtttcc
      781 gtgggcgccc ataaatgcgg caccacggcc agcagcggta ccttgtgttt ttctgaaatc
      841 attgcagcga gtcgcaggcg cgggtccagg ttgagcttgg cataaagcag ccccagggga
      901 agcaatacgc ccagcaccag accaccgatg acaaaatgca gaaagcgcag accgctgggt
      961 gacagcggca aggtggccgg ctcctgaatc ttgatcgtga gaccctgctt ttccttgtcg
     1021 agattcatcg acacgcgcgc gttttcacgg cgtttcagca ggtcctgata gatatcgcgg
     1081 ttgacctggt aatcacgtgt cagctcggcc agggtcgcct cgccgccgtg cacgcgccgc
     1141 ccgcgctcca gttcctgttg caactgccgc ttggcctcgc tgatacgggc gctgagcatt
     1201 tcgatattga cacgcgtctg ggagagctcc tgcttgagct gctgatacat cgggttattg
     1261 atcacgccct cgtcgatgac gaccttgccg ctggccttga cggtttcgcg atgctgcctt
     1321 tcctcgacaa tggcctgatt caggtcttcg atctggtgct tgatctggat gatgtccggg
     1381 taattgtcgt gatagctgag gcgcaaggtt tcaagtcgcg attgcagttc cgcgatgcgc
     1441 gaacggtact gaccctcacg ggaaagcgcg gtggcaacct cggcctcgcc ggaaagctgt
     1501 ttctcgagtg acagcttttt gacctcggcc tctttcaggt cctggctgga ctgctcgatt
     1561 ttcgtctgaa gcagattcag gcgcgtgctg atatccgcgt cggtgcccgg tctggcgtcc
     1621 agattggcgg aacggaattc cttcagctgc tcctctgcct tgagcagctt gtcgtgatat
     1681 tcctgggtct gcttgtcgat aaattcgaaa gcggcccggc tttcgtcgat cttggtgcca
     1741 agactttcgg aaatgaacag ctcggcgtat ttctgggtag tcctgaaggc gcgttccgga
     1801 tcatcgtcct tgtattcaat cttgatcagg tccttgcccg cgccggtgat ggtcgtgcgc
     1861 ctggaaagtt tctcgatgat cttttcctgt tcctcgggac tcggattacc cttcatccag
     1921 ccggcgtgct ccaggatctg gctcatgatt ttccggccga gtatcacttc ccgcgccagc
     1981 cgcgagcgat cagccacctc cgtggcgacc gccgcaccct gcatcaatgg ctgaatgatt
     2041 ttcttgtcct cgacaaggat ggtggtcgaa gatgtatagc ccttgggcca gatcagaccc
     2101 aggcccaaca taagcagatt gatggcgaaa aaaccgatca ccacggcctt ccggtaaaaa
     2161 aaggcctcgt tgaccagcac cttgaaaatc tgatctggac gcaggttcat agcatcaccc
     2221 ccaaacctag aacagtctct cgggaatggt taccacgtcg ccaggtttca gctcaaaatt
     2281 ggattcgagt ttccccttgg tcaggatatc ggcgagatcg acgtcgaata cttcggtttt
     2341 tgtttttgtc ttgcggtaca gcttggtgcg gttcggcgag gcgaaatcgt tggtgccgcc
     2401 cgcctcgagt accgcgtcaa gcaccgtcat gcccggacga tagggcatgg aacgcggggt
     2461 gcgcacggcc cccgtaaccc gcacacgcga cagaaattca tggctgcgca actccgtcag
     2521 aataaccgca acattcggtt cacggatgta cgccgacagc ttgtccttga tgatcgccgc
     2581 gacctgcgtg ggtgtgttgc cgcccgcctg cacatcgccg atcaagggca ccgatatttt
     2641 accgtccggt cgcaccggga cggtcgttga cagttccgga ttgcgccata ccgtaatctg
     2701 gagacggtca tcgacaccaa tgcggtagtc accctccaga agcgatgcaa accggtttac
     2761 ctcctccttg ccttcggtgg catccggcac agacttgcag cccgaaacca ccagaaacat
     2821 ccctgccaac agcactgaaa caaggcgaaa caaattcatt tttacctgct tattcattct
     2881 aaaccccaac aagcaaaggc gccgtgaacg gcctggtacc tcgatactaa ttcacctttg
     2941 gcgctttgtt attggtattg cgagggcaat tcaactgtcg gtttgtttgc cggcctgtac
     3001 aaaccggtag atccaccacc aatcgaacga tgatgccgcg tcctgtccta ccgctccaca
     3061 aacgacatgc gggattatac agttaatccc ggtaaatgtg acctgctaaa aaaccggcag
     3121 ggaaatttca tcgcgaaaca tgaggttatc ttgattgctc agcaatgtca tcccgccccg
     3181 ggatagcggg caaaatttgc accctgcccc tataattgat ggattatagc gatcccgccc
     3241 gggaatccat cacataccct cacaaccgct gacaacccgc ctgcgagtta acggaaaaac
     3301 gttcagagac cagatgtagc catgccagac aattacctga agaactacct catactcggg
     3361 gttaaacccg gagccagttg gaaacaactg cgtcaggcct acaaaaaact ggtcaacgtc
     3421 tggcacccgg accggttcca gcaggatatc cgccagaagc ggctggccga ggaaaaaacc
     3481 aaggagatta cccaggcgta caaggaactg gccgaatatc acaaaaaatt cggcgtcctg
     3541 cccctgcctc ccgaagaaga aatacccccc gcccgcgaaa acaacgcgag cccggctacg
     3601 cccgcgaaga gcacagctcc cgacactgtc aaaaccccgg aaccagcttc cgcgccatcc
     3661 ggcacgaaaa ttccgaaaca ccgaatgtcc aaacggcgcg cgcgcgctct tgcggccatg
     3721 gcgctgctcg gatccatcta ctttatatgg cagtttgccc cgtgggagcg acaggataac
     3781 gcgcctgcga atgaggggca tggcgatcaa tcgtcgaacc atcaccatga aaccgaatcc
     3841 gtcgacgtga cgccagccga aaaacgcttt acggtcggct cgtccctcgg cgaggtgtac
     3901 gccatccagg gcgtgcccac gaaaacggag aaagacatct ggtactacgg caactcaaag
     3961 gtctactttg ccaagggaaa ggtgcttcgt tgggaagaaa aaaccgatag tcccttgcgg
     4021 gttgacgtca tatccggttt tgaaaaaacc actgccgggt ttttcggacg aggttcaacc
     4081 aaggaggaag tgttggcggt acaaggcagg cccgaccgcg atgccggcag tgtctgggat
     4141 tacggtcttt cccgggtcta ctttgagaaa gatcgcgtga ccggatggca tgaggcgcag
     4201 tacaacccgc tgcgggtaag aaattaactc gcctcaacgg cggtcagcgg gcgcccttcc
     4261 cgaaaaaaat cacttgcgcg gtctggaaaa gaataatcag atccagaaac agtgtgtggt
     4321 tcttcacgta ataaagatcg tactgcaatt tttgcacggc atcctccacg gtggcgccgt
     4381 agggataacg tatctgggcc catccggtaa tgcccggctt gaccgtatgg cgtgactggt
     4441 aatacgcgat tttcttcgac agatcctgca cgaaataggg tctttccggg cgcgggccga
     4501 cgaaactcat gtcgcccctg aacacgttga acacctgcgg caactcatcg atccgcagca
     4561 ggcggatgat tctgcccacg cgcgtcaccc ggtcgtcctg tttttttgcc cactgcggca
     4621 cgccatcgcg ctcggcgtcc tgacgcatgc tgcggaattt caggacgtta aaaacccgtc
     4681 catgcgcgcc aacgcgttcc tggcggtaaa aaaccgggtc accgctttcc agccagatca
     4741 gcgaggccgt tatcagcatc accggcaggg taagcagcag caatacaccg ctggcagcga
     4801 catcgaacat acgcttggtg acttccttga gcggactccg gccgaaaccg tcggaaaaga
     4861 tcatccagct cggattgagc gagtcaacct gaatctggcc cgtctcgcgc tcgaagaagg
     4921 aaggcagatc gaccacctcg atgccggcca gcttgcattc cagcaactga tccgcgggca
     4981 atccgccgcc gcgtcgctcg cgcacaccca cgataatttc atcgatcttg tatttcctgg
     5041 cgacggaaag aatggggccc ttgtcatgca atacccgggt atgatcgaga cagcaatccg
     5101 agttggtgag gggcaaacag cccacaagat tgaatttgcg ctccccattc tcgtctcgct
     5161 ccagtccttc tatcttggcg gagcggctgc ctgcccccag cagcaacacg cgccggtgca
     5221 tggcatccaa cttgaacagc cgcataaaga tcagccgcgt cagagcggtg cccacgaagg
     5281 caaacaggaa caccaggcca aacgcgccgc gaccgagatg cagattcgga atggcataga
     5341 agatcagcag catggtcacc agaccgacca cgaaactggc catcaaccgt ataagatggc
     5401 cccactcccc ttcctgtctg acatcgcgct gatgcagccc gagggcggtc atgatggcaa
     5461 gcatgaccgc ggtgaatacc agcgcgcggg gcagtatcgg gccaatcaaa ttgatatcgc
     5521 cgggatcttc ccagacaaaa cgtatgcccg tgccggcata catggaggca aaaagcaccg
     5581 ccgcctcggt aatggccatg aggaaaagcg gtaacggcac atagtgcttg aacagtcgga
     5641 tcatagagcg actctgtcaa tcctttacaa aggctccgta acgttgccca tcacccggat
     5701 tcgagatttc cggtctgtcg cgacagaccg aattcgggtt ctcaatgcca tccacatgga
     5761 tagccctgag aagaaacagt tactttgcag gattcagcaa ttactgtgcc agacatacaa
     5821 atcattctta tttttctcaa aattctcgaa gaaaattccg cccggatcaa cacaattttg
     5881 aaccggatgg taaaaagcaa aaaaggggcc aatagggccc cttttatatt tggataccgg
     5941 agagattgct cgccccatcc ctggggctcg ccgcttcgcg gccagcctac ggctggccaa
     6001 atttgctccc ggcaaatttg tcgaactccc gacccctttg gggcctggaa gttcgcaaac
     6061 ctcgataccg catttataca aaaggggcca agggggccct tttgtataaa tgcgggctgg
     6121 tgaaaatggc gcgcccggag agattactcg ggccttcggc cctcgcccct ccggggccgg
     6181 cgggg
//
LOCUS       DBNGZE010000338         4561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1228121, whole genome shotgun sequence.
ACCESSION   DBNGZE010000338 DBNGZE010000000
VERSION     DBNGZE010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1228121"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="AC16FV_08745"
     CDS             complement(<1..160)
                     /locus_tag="AC16FV_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR2850180.1"
                     /translation="MSAKFELTAEPRSAKGTGASRRLRRSGKVPAVLYGAGKQPTMVA
                     LDHNLMTRH"
     gene            complement(259..1194)
                     /locus_tag="AC16FV_08750"
     CDS             complement(259..1194)
                     /locus_tag="AC16FV_08750"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR2850181.1"
                     /translation="MVVFTGNANPALAELVVRHLGLPLGKAVVGRFSDGEIQTEVMEH
                     VRGKDVFIIQPTCAPSNDNLMELLIMIDAVKRSSARRITAVIPYYGYARQDRRPRTAR
                     VAITAKLVADMITVSGAHRVLTMDLHADQIQGFFNIPTDNVYASLVLLGDIWRQEFKD
                     LLVVSPDVGGVVRARAMAKHLEADLAIIDKRRPRPNEAKVMHIIGEVEGRSWVLLDDL
                     VDTAGTLCEAAGALKEHGAKKVLAYCTHPVLSGSAVERIGKSQLDELVVTDTIPLKPE
                     AKACRRIRQLSVAELLAESMRRIAEEDSVSELFME"
     gene            complement(1465..1539)
                     /locus_tag="AC16FV_08755"
     tRNA            complement(1465..1539)
                     /locus_tag="AC16FV_08755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Gln,seq:ttg)
     gene            complement(1711..2532)
                     /gene="ispE"
                     /locus_tag="AC16FV_08760"
     CDS             complement(1711..2532)
                     /gene="ispE"
                     /locus_tag="AC16FV_08760"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR2850182.1"
                     /translation="MKTWPAPAKLNLFLHVTGRRADGYHTLQTVFQFLSYGDELAFDV
                     TDDGRITRAAPVPGVAEEQDLTLRAAHLLKESCGVTKGAQIHLTKRLPIGGGLGGGSS
                     DAATTLLALNELWGTRLTITELAALGLKLGADVPVFIHGHAAWAEGIGEILTPVEPEE
                     AWYLVVVPPVHVSTAQVFADPELTRHSPPLTIRDFHEGRGLRNDLEPVVRSRYPEVDR
                     AMRLLTEFGAPRMSGSGGCVFLKVTDADRGRQILERIPKPFTGFVARGLNRHPCR"
     gene            complement(2633..3265)
                     /gene="lolB"
                     /locus_tag="AC16FV_08765"
     CDS             complement(2633..3265)
                     /gene="lolB"
                     /locus_tag="AC16FV_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR2850183.1"
                     /translation="MRPVLGLIIIFVSLISGCATAPLPPAMDINPAWQARQAALMSVT
                     AWNIRGRLALRAAEQGGHATLNWKREGERHRLDFIGPLGRGHLRLTQDSHGAELRDAD
                     KHVWRAADAGRLLYRATGWIVPLADMNYWVLGLPAPGATPDRQLDEQGRLKRLTQSGW
                     DIQFLEYTRYGAFDLPSKLYITRQDRHAETNSDTGETLEVRLSIDNWTLQ"
     gene            complement(3262..>4561)
                     /locus_tag="AC16FV_08770"
     CDS             complement(3262..>4561)
                     /locus_tag="AC16FV_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850184.1"
                     /translation="TLFAAEETRRNLDQAYLRVAAVLGRASNRAAALDVMQSLVALNP
                     RVASAHFAMAHLLVRGGDLEKALVSVEEALKQRPDWEEAALFKARILLSQKEPQKARA
                     FYESFLSDNRGANSVRLNYARFLVDQKEWQKALEEFKRVADNAPDDADTVYAVGLLSL
                     QTNNLEDADKYLKQALKLRPENDQARLYLGQVAEQRKQYGEAVKWYLEVKDGEYYFEA
                     QARLGVAFAKQGDLARARRHLDGIKAQNEAQRVQVALAHEQILRDARQFNEALAVLNA
                     AIQTVPGDKDLLYARALIAEKLHMLEITERDLREILKKDPKNVNALNALGYTLTDRTT
                     RYDEALELLQQAMELKPDDAFIMDSMGWLQYRRGNSAEAVKYLRRALDIRNDAEIAAH
                     LGEVLWVIGDRHEAESVWDRALRETPDNEALNGVIKKFKP"
BASE COUNT          827 a         1500 c         1325 g          909 t
ORIGIN      
        1 agtggcgcgt catcaggttg tggtccagcg cgaccatcgt cggttgcttg cccgcgccat
       61 acagcacggc gggtactttg ccactgcggc gaaggcggcg gctcgcaccc gtacctttcg
      121 ctgagcgtgg ctcggcagtc aattcaaatt tagcgctcat tgtcagtctc cagtaaggtt
      181 aaacaactcc ccctttcccg cgaccaggat cggagggtac agaaaaggca cagggccatc
      241 gcccaacgcc cgaaatcgtt actccatgaa caactcgctg accgaatcct cttccgcgat
      301 gcgccgcata gactcggcca gcagctcggc tacgctcagc tgacggatgc gccggcaggc
      361 tttggcctcc ggcttgagcg ggatcgtgtc ggtcaccacc agctcatcga gctgggactt
      421 gccgatccgt tccaccgcgc tgcccgacaa caccgggtgc gtacaatatg ccaatacctt
      481 cttcgcgccg tgctccttga gcgccccggc cgcctcgcac agcgtcccgg cggtgtcgac
      541 gaggtcatcc aacagcaccc agctacgacc ttccacctcg ccgatgatgt gcatcacctt
      601 ggcctcgttc ggcctcgggc ggcgcttgtc gatgatcgcc aagtccgcct ccaggtgctt
      661 cgccatcgcc cgggcccgca ccacgccgcc gacgtccggc gacaccacga gcaggtcctt
      721 gaactcctgc cgccatatgt cgccgagcag caccagcgat gcgtatacgt tgtccgtcgg
      781 gatgttgaaa aatccctgga tctggtcggc atgcagatcc atggtcaaca cccggtgcgc
      841 gccggaaacg gtgatcatgt ccgccaccag cttcgccgtg atcgccaccc gcgccgtgcg
      901 cggccggcgg tcctggcgcg cgtatccata ataaggtatc accgccgtga tgcgccgcgc
      961 cgaggagcgc ttcaccgcgt cgatcatgat cagcaactcc atcaggttgt cgttgctggg
     1021 cgcgcaggtc ggctggatga tgaacacatc cttgccacgc acatgttcca tgacctcggt
     1081 ctggatctcg ccatccgaga agcggccaac gaccgccttg ccgagcggca atcccaagtg
     1141 ccgcaccacc agttccgcca gtgccgggtt ggcgttcccg gtaaacacca ccatgttgtc
     1201 caaggacacg tgatctcccc tgtgagtcgg atggtccgcg acaaaatctg gttggggcgc
     1261 cgataccgct ccgccaatgc tgaaccattt tactggttgg ggcgccagga ttgttcgccc
     1321 catccctggg gctcaccctc gccgcctcgc ggctcgggcc agtcgcttcg cgactggcca
     1381 aattcgctcc cggcgaattt gtcgaacctt ccaggttctc atcctggcta ccaattcaaa
     1441 aatgcccaat gggcattttt gaattggttg gggcgccagg attcgaacct gggaatgccg
     1501 gaatcaaaat ccggtgcctt accgcttggc tacgccccaa gaatctgcgc cccacataat
     1561 cacccgcgct cttggcacag ccattcgaac ctgggtcgct ctcggcatcc tgcctctcgc
     1621 gacattagta cgtccctgta cgtcagaatg ccggggataa attcagtacc ttaccgcttg
     1681 gctacgcccc aaggtgaact gtcgccgcgt ttaccgacag gggtgccgat tcaatccccg
     1741 ggccacaaag ccggtaaacg gtttgggaat tctctccaga atctggcggc cccggtccgc
     1801 gtccgtcacc ttcaggaaca cgcaaccacc ggaacctgac atgcgcggcg caccgaactc
     1861 ggtcaacagc cgcatcgcgc ggtccacttc cggatagcgg cttcgcacca ccggttccag
     1921 gtcgtttcgc aggccgcgcc cttcgtgaaa gtcgcgtatt gtgaggggcg ggctatgacg
     1981 tgtcaattcg ggatcggcga aaacctgtgc cgtggagaca tgcaccggcg gcaccaccac
     2041 cagataccac gcctcttccg gctccaccgg ggtcagaatc tcgccaatcc cttcggccca
     2101 ggcggcgtgt ccgtggatga acactggcac atcggccccc agcttcaatc ccagcgccgc
     2161 cagctccgtg atggtcaggc gcgtgcccca caattcattc agggccaaca aggtcgtggc
     2221 ggcatcggag ctgccgccgc caagaccgcc gccgatggga aggcgtttgg tgagatgaat
     2281 ctgcgcaccc ttcgtgacgc cgcacgactc ctttaacagg tgcgccgcgc gcaacgttag
     2341 gtcctgctct tcggccacac cgggcacggg agctgcgcgc gtgatccggc catcgtccgt
     2401 cacgtcgaat gccagctcat cgccgtagct gagaaactga aaaaccgttt gcagggtatg
     2461 gtagccgtcc gcgcggcggc cggtaacatg cagaaacaga ttgagcttgg ctggcgccgg
     2521 ccaggttttc atggatggat cgtaagctgt aaatcgtgag ccgtaaattg tgaacaggaa
     2581 gccggcgagg tgcaggttac accttcacga ctcacgattc acgtcttacg gtttactgca
     2641 gcgtccagtt atcgatgctg agccgcacct caagcgtttc accggtatca gagttagtct
     2701 ccgcgtggcg gtcttgccgg gtgatataca gtttgcttgg caggtcgaaa gccccataac
     2761 gggtgtattc aagaaactgt atatcccagc ccgactgtgt cagccgtttc aggcgccctt
     2821 gctcatccag ttgccgatcc ggcgtcgcgc ccggggcagg caggccgagc acccagtaat
     2881 tcatgtcagc gagcggcacg atccagccgg tggcacggta caggagacgt ccggcatccg
     2941 ccgcacgcca gacgtgcttg tcggcatcac gcaactcggc cccatgactg tcctgtgtca
     3001 gacgcagatg accgcggccc agcgggccga tgaaatccag acgatgccgc tcaccctcac
     3061 gcttccagtt cagcgtggca tgcccgccct gttcggcggc acgcagggcc agccttccgc
     3121 ggatattcca ggcagtgacc gacatcaggg cggcttgccg cgcctgccag gcaggattga
     3181 tatccatagc cggcggcaat ggcgcggtgg cgcaaccgga gatgagcgaa acgaaaataa
     3241 taatgaggcc gaggaccggc ctcatggttt gaatttcttg atgacgccgt tgagcgcttc
     3301 gttgtccggt gtctcgcgca gcgcgcggtc ccacaccgat tcggcctcgt gacggtcgcc
     3361 tatcacccac agcacctcgc cgaggtgcgc cgcgatttcg gcgtcgttgc ggatgtcgag
     3421 cgcgcgccgc agatatttca ccgcttccgc gctgttaccg cgccggtatt gcagccatcc
     3481 catgctgtcc atgatgaagg cgtcgtccgg cttgagctcc atcgcctgtt gcagcagctc
     3541 cagcgcctcg tcgtaacggg tggttcgatc cgtcagggtg taacccaacg cgttcagggc
     3601 gttgacgttt ttcggatctt tcttgaggat ttcgcgcaga tcacgttcgg tgatttccag
     3661 catgtgaagt ttttccgcga tcagggcacg cgcgtacaac aggtccttgt cccccggaac
     3721 ggtttgaatc gccgcattca gcaccgcgag cgcttcgttg aattgccgag cgtcgcgcag
     3781 aatctgctca tgcgccagcg ccacctgtac gcgctgggct tcgttttgcg ccttgatgcc
     3841 gtccagatga cgccgcgcgc gggcaagatc gccctgtttc gcgaacgcca cgccgagacg
     3901 ggcctgcgcc tcgaaataat attcgccgtc ctttacctcg agataccact tcaccgcctc
     3961 gccatattgc ttgcgctgtt ccgcgacctg tccgagatag aggcgggcct gatcgttctc
     4021 gggacgcaat ttcagcgcct gcttcaggta tttatccgcg tcttccagat tgttggtctg
     4081 caatgaaagc aggccgacgg cataaaccgt gtcggcatcg tccggggcgt tgtcagcgac
     4141 gcgcttgaat tcctcgagcg ccttctgcca ttccttctgg tcaaccagga agcgcgcata
     4201 gttcaggcgg acgctgttgg cgccgcgatt gtcgctgaga aacgattcat aaaaggcgcg
     4261 cgccttctgt ggttccttct gcgacagcag gatgcgtgcc ttgaacagcg ccgcctcctc
     4321 ccaatccggg cgctgcttca gcgcctcctc gacggagacg agcgcctttt ccaggtcgcc
     4381 gccacggacc agcagatgcg ccatggcgaa atgggccgaa gcgacgcgcg ggttcagcgc
     4441 gaccagggat tgcatcacat cgagggccgc ggcgcggttg ctcgcgcgcc ccagcacggc
     4501 ggccacgcgc aggtaggcct ggtcgagatt gcggcgggtt tcctccgcgg caaacagggt
     4561 t
//
LOCUS       DBNGZE010000339         3235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1020402, whole genome shotgun sequence.
ACCESSION   DBNGZE010000339 DBNGZE010000000
VERSION     DBNGZE010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1020402"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16FV_08775"
     CDS             complement(<1..259)
                     /locus_tag="AC16FV_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR2850185.1"
                     /translation="MGLIGLGRMGANMARRLRKGGIEVVALNRNADVTKDLAKETGLK
                     AANSLEDLVSRLKTPRTVWLMLPAGDVTQQHLDQLVSRLAPG"
     gene            complement(329..973)
                     /gene="rrtA"
                     /locus_tag="AC16FV_08780"
     CDS             complement(329..973)
                     /gene="rrtA"
                     /locus_tag="AC16FV_08780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HIR2850186.1"
                     /translation="MEAATRLTAVRLPLWTLAICASAGLVFLLPFLESVLIYDRSAIA
                     QGELWRLVTGNLVHYSAAHLGYDLLALLVAGTMIEMSGYRHYPALCLAAATCIGMVLY
                     WAEPAMLYYAGLSGVATAAIVYLCLHGLTEKGAWRWICAGALLGLGIKVGIEFMLNES
                     LLVASGSSEFQPVPLSHLVGAGVALFLFVLSRLRIRNYRSSASRYKPLPERHPA"
     gene            complement(952..1827)
                     /gene="rhlP"
                     /locus_tag="AC16FV_08785"
     CDS             complement(952..1827)
                     /gene="rhlP"
                     /locus_tag="AC16FV_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525145.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HIR2850187.1"
                     /translation="MKWTIPAVSLSILVALFTVVSVTGCATGQTRHATSTVDYLYPNT
                     KDPIVTPDIPVLTLPMRVGIAFVPGDGGGNYGRGLMSVRNQSFILTEKKKMDLMQEVA
                     NHFKKYPYVKDIELIPSAYLTPRGSFANLDQIRTMYGIDVIVLLSYDQVQFTDEGMLS
                     LTYWTIVGAYVIPGERNDTHTMLDAVVYDIKSRKMLFRAPGTHHIKGKATLVNLSEEL
                     RANSETGFNEAAKSMIVNLDQQLAVFKERVKERPTEYKVVRSAGYSGGGSLDLVGLAM
                     VFGLGGYFLWKRRRA"
     gene            complement(1933..2529)
                     /locus_tag="AC16FV_08790"
     CDS             complement(1933..2529)
                     /locus_tag="AC16FV_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR2850188.1"
                     /translation="MGKNRMEAFSDVVLAIIITIMVLEMKVPHGTDFAALRPLLPVFL
                     TYVLSFIYLGIYWNNHHHMLHVTQHVSGAILWANLHLLFWLSLFPFVTGWMGENHFAV
                     LPTALYGVVLLMAAIAYWILQQAIIARHGPESLLAKAVGRDLKGRLSPVLYIVAIVAA
                     FFAPWVAGALYVLVAVLWLVPDRRIERALAARKGQNLS"
     gene            complement(2540..2938)
                     /locus_tag="AC16FV_08795"
     CDS             complement(2540..2938)
                     /locus_tag="AC16FV_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2850189.1"
                     /translation="MEPRLSFVTLGVSDLQRATRFYEEVLGLPRIPTPPEVTFFELGK
                     TWLALYPRELLAADAGVPAAGSGFPGFTLAHNVRSAAEVDAFLKEVAARGGRIVKPGT
                     RADWGGYTGYFADPDGFLWEVAWNPQFPHT"
     gene            complement(2951..3202)
                     /locus_tag="AC16FV_08800"
     CDS             complement(2951..3202)
                     /locus_tag="AC16FV_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR2850190.1"
                     /translation="MDITGLLIFLAIGAVAGWLAGTFMKGRGFGLLGNIVIGIIGAVL
                     GGFVFGLLGITAGGLIGSIVMATVGAALLLFIIGILKKA"
BASE COUNT          740 a          982 c          899 g          614 t
ORIGIN      
        1 cgcccggcgc cagccgggag acgagttggt cgagatgttg ttgcgtcacg tccccggcgg
       61 ggagcatgag ccagacggtg cggggagttt tgaggcggga gaccaggtct tcgagactgt
      121 tcgcggcctt gaggccggtt tccttggcga ggtccttggt gacatcggca ttgcgattga
      181 gcgcgacgac ctcgatgccg cccttcctca accgccgggc catgttggcc cccatgcgcc
      241 ccagaccgat cagtcccatt tccacggcca tcgtctcccg atttctcctt gcaggaacga
      301 tgaccttaac acgaacaggg agtcgctgct aagccggatg ccgctccggc aacggtttat
      361 agcggctggc tgagctgcgg taattcctta ttctcaagcg cgacaataca aacagaaata
      421 atgcgacgcc cgcaccgacg aggtggctga gcggaactgg ctgaaattcg cttgaaccgg
      481 aggcaaccaa caaggattca ttgagcatga attcaattcc cactttgata ccgaggccaa
      541 gcaatgcgcc ggcacatatc caccgccacg cgcctttttc ggtcaggcca tgcaagcaca
      601 gatacacgat cgccgccgtc gccaccccgg aaagaccggc atagtagagc atggcgggct
      661 cggcccagta gagaaccatg ccgatgcatg tcgccgcggc aagacacagc gccgggtaat
      721 gccgataccc gctcatttcg atcatggtgc cggcaaccag caatgccagc aggtcgtagc
      781 cgaggtgagc ggcggagtaa tgcacgaggt tgccggtcac gagccgccag agctcacctt
      841 gggcaatggc gctccgatca taaatcaata ccgactccag gaacggtaag agaaaaacca
      901 gtcccgcgct ggcgcagatc gccagcgtcc agagcggcaa gcgcactgct gtcaggcgcg
      961 tcgccgcttc cacaggaaat atcccccgag gccgaacacc atcgctagcc cgaccagatc
     1021 aagacttcca ccaccgctgt aacccgcgga gcgcacgacc ttgtattcgg tgggccgttc
     1081 cttcaccctc tccttgaaca cggcaagctg ctgatcgagg ttgacgatca tgctcttcgc
     1141 cgcctcgttg aagcccgttt cactgttggc gcggagctct tcgctgagat tcaccagcgt
     1201 ggccttgccc ttgatgtgat gcgtgccggg cgcccggaac agcatcttgc ggctcttgat
     1261 gtcgtagacg accgcatcga gcatggtatg ggtgtcattc ctctcgccgg gaatgacata
     1321 ggcccccacg atggtccagt aggtaagact caacatgcct tcgtccgtga actgcacctg
     1381 gtcgtaggac agcaggacga tcacgtctat cccgtacatg gtgcggatct ggtcgaggtt
     1441 ggcgaaactt ccacggggcg tgagataggc cgacgggata agctcgatat ccttgacgta
     1501 gggatatttc ttgaagtgat tcgccacttc ctgcatcagg tccatcttct tcttttccgt
     1561 gaggatgaaa ctctgattac gaaccgacat cagtccacgg ccataattcc cgccgccatc
     1621 gccgggcacg aaagcaatac ctacccgcat cggcagcgtc agcacgggaa tgtccggcgt
     1681 gacgatgggg tccttggtgt tggggtagag ataatcgaca gtgctggtgg cgtgccgtgt
     1741 ctggcccgtg gcacaaccgg taaccgacac gacagtaaac aaggcgacaa gaatggaaag
     1801 cgaaactgcc gggattgtcc acttcatgga aaaacctccg tagcttggtt ttatagtttg
     1861 agcgtgtttc ttgtgcttta agtgtgcggg aaccgcaggt ttcaggcaac ttcccggaac
     1921 cgaaaattac cgttaagaca aattttgccc tttccgcgcg gccaatgcgc gttcgatgcg
     1981 ccggtcgggc accagccata acaccgctac cagaacatac aatgctccgg ccacccacgg
     2041 cgcgaaaaac gcggcaacta tcgcgacgat ataaagcacg ggcgacagcc tccccttgag
     2101 gtcccggccc acggccttgg caagcaggga ttccgggcca tgcctggcga tgatggcctg
     2161 ctgcaaaatc caataggcta tggcggccat cagcaaaacc acgccataca aggccgttgg
     2221 taacaccgca aagtgattct cgcccatcca tccggtcacg aaagggaaca gcgacagcca
     2281 gaacagcaga tgcaggttgg cccacaggat cgcaccgctc acatgctgcg tgacatgcag
     2341 catgtggtga tggttgttcc agtaaatgcc gagatagatg aagctcagga cgtaagtgag
     2401 gaacaccggc agcaacggac gcagggcggc gaaatccgtg ccatgcggca ccttcatttc
     2461 cagaaccatg atggtgatta tgatggcgag cacgacatcg ctgaacgcct ccatgcggtt
     2521 cttgcccatg atcagccttt tatgtgtgcg ggaactgcgg gttccaggca acttcccaca
     2581 gaaaaccgtc gggatcggcg aagtaacccg tatagccgcc ccagtccgcg cgcgtgcccg
     2641 gcttcacgat gcgcccgccc ctggccgcga cttccttcag gaacgcatcc acttccgccg
     2701 ccgaacgcac attgtgcgcc agcgtgaacc cgggaaaccc cgaccccgcc gccggaaccc
     2761 cggcatccgc cgccagcaac tcgcgcggat atagcgccag ccaggtcttg cccagctcga
     2821 agaaggtaac ctcgggcggc gtgggtatac gtggaaggcc cagtacttcc tcataaaacc
     2881 gcgtcgcgcg ctggagatcg gagacgccga gagttacgaa actgagacgg ggttccatgt
     2941 tgtcaggtca ttatgccttc ttgaggatgc cgataatgaa cagcagcaat gcagccccaa
     3001 cggttgccat aacgattgaa ccaattaatc cacccgccgt gatgccaagc agaccaaata
     3061 caaaaccacc caatacggcg cctatgatgc caataacgat gttaccgagc aggccaaaac
     3121 cacggccttt catgaatgtt cccgccaacc agccagccac ggcgccaatc gcgagaaata
     3181 tcaacaatcc tgttatatcc atgatttaac ctccgtgtcg ttatgaattg agctt
//
LOCUS       DBNGZE010000340         2748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1437731, whole genome shotgun sequence.
ACCESSION   DBNGZE010000340 DBNGZE010000000
VERSION     DBNGZE010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1437731"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1008
                     /locus_tag="AC16FV_08805"
     CDS             28..1008
                     /locus_tag="AC16FV_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2850191.1"
                     /translation="MNQNTLVEIKNLSRFYGALQAVKNVSFTIRQGEVLGFLGPNGAG
                     KTTTMQIISGNLAPSGGSVTIAGHDLLEDPRAAKSQIGYLPEQPPLYRELTVDEYLDY
                     CAALNHVPRARRVQARENAKERCGLHDAGRRLIGNLSKGFQQRVGIAQAIIHLPPVVI
                     LDEPTVGLDPIQIREIRMLIRELGKEHGVILSTHILPEVQATCDRVQIINKGELVLNE
                     SIEGLEHHMKSASLTVAFHHPPARETLEKLPGVKSVQSEKDGRMHVFHEAEQNPTDAI
                     LRQAVEKNWGLYEIRPGRFSLEQIFMDLTTDAAAENAHAFEPAFTTGSQP"
     gene            1005..1754
                     /locus_tag="AC16FV_08810"
     CDS             1005..1754
                     /locus_tag="AC16FV_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR2850192.1"
                     /translation="MILTIAIREFKSLFLSPLAWSILAVVQVILSFLFLSRLEVFQLY
                     QPQLMAMEGAPGLTEIVLPDLLGNAAVILLLVVPLLTMRLVAEERRNRTLALLFSTPL
                     SMTEIVLGKYLGILLFLLVLLGMIALMPLSLLAGASLDLGLMASGFLGLALLLAGFAA
                     VGLFMSTLTQYTTVAAISSFGALLLFWILDWSGQGFTGGNWLAYLSLFNHYKPFLEGI
                     FDSADAAYHILLITTFLVLSIRRLDADRLGG"
     gene            1783..>2748
                     /locus_tag="AC16FV_08815"
     CDS             1783..>2748
                     /locus_tag="AC16FV_08815"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR2850193.1"
                     /translation="MYHTPKTHWQLRLMNLIFVVLFLLAVGLLQWLAKEYHLQFDWTR
                     MHRHSLSPASKAVTERLKEPLKITAFASQRGETRRQIQEMVGRYQKHKPDIQLEFVDP
                     DTAPERVRAAGVQLDGELVFEYGDARENLPPTQFNEQSFTNALTRLGHRGERWVVFLS
                     GHGERSPDKQANFDLSTWAAQLRKRGFQTKTQSLGEHPQIPQNTTVLVIAGPRTRLLA
                     GEVKEIQNYLNRGGNLLWLQDPGPLLGLEPLAENLGIEFQPGVIVDPASEDITGNAAA
                     IVVTKYGAHPVVRGFADNTVFPQAGGISLNAPEGWQGSVLMDTRPS"
BASE COUNT          547 a          882 c          824 g          495 t
ORIGIN      
        1 gacccaaagc gcagaccgcg gcacgagatg aaccaaaaca cactggtcga aatcaaaaat
       61 ctctcgcgct tctacggcgc gctacaggcg gtcaagaacg tcagcttcac catccggcag
      121 ggagaagtgc tgggtttcct cggccccaac ggcgccggca agaccaccac gatgcagatc
      181 atcagcggca acctcgcgcc ctcgggcggg agcgtgacca tcgccggcca tgacctgctc
      241 gaagacccgc gcgccgccaa gagccagatc ggctatctgc cggaacaacc gccgctctac
      301 cgcgagctga cggtggacga atacctcgac tactgcgcgg cgctgaatca tgtgccgcgc
      361 gcgcggcgcg tgcaggcgcg cgagaacgcc aaggaacgct gcggcctgca cgacgccggt
      421 cgacgtctga tcggcaatct gtccaagggc ttccagcagc gcgtcggcat cgcgcaggcg
      481 atcattcatt tgccgccggt ggtgatcctg gacgagccca cggtcggcct cgacccgatc
      541 cagatccgcg aaatccgcat gctcatccgc gaacttggaa aagagcacgg cgtgattctg
      601 tccacgcata tcctgcccga ggtgcaggcc acctgcgacc gcgtgcagat catcaacaag
      661 ggcgaactgg tgctgaacga aagcatcgag ggcctggagc atcacatgaa atcggcctcg
      721 ctcacggtcg ccttccatca cccgccggca cgggaaacgc tggaaaaact tccgggcgtg
      781 aaatcggtgc aatcggagaa agatggtcgg atgcacgtat tccatgaggc cgaacaaaac
      841 ccgaccgacg ccatcctgcg tcaggcagtc gagaagaact gggggcttta tgaaatccgt
      901 cccggacggt tttcgctcga acagatattc atggacttga ccacggatgc cgcggcggaa
      961 aacgcgcacg ccttcgagcc agccttcacc acgggtagtc agccatgatc ctgaccatcg
     1021 ccatccgtga attcaaatcg ctgtttctct cgccgctggc atggtccatt ctcgcggtgg
     1081 tgcaggtgat actcagcttc ctgttcctga gccgcctcga ggtgttccag ctgtatcagc
     1141 cgcaactaat ggccatggaa ggcgcgccgg gcctcacgga aatcgtcttg cccgacctgc
     1201 tcggcaacgc cgcggtaatc ctgttactcg tcgtgccgct gctgaccatg cgcctggtgg
     1261 cggaggaacg gcgcaaccgc acactcgcgc tgctgttcag cacgccgctg tcgatgaccg
     1321 agatcgtgct cgggaaatat ctcggcatcc tgctattcct gctggtgctg ctgggcatga
     1381 tcgcactgat gccactgtcc ctgctggcgg gcgcgagcct ggatctgggc ctgatggcct
     1441 cgggttttct cggtctcgcg ctactgctgg cgggattcgc tgccgtgggg ctgttcatgt
     1501 ccacgctcac gcaatacacc acggtcgcgg ccatcagcag cttcggcgcg ctgttgttat
     1561 tctggattct tgactggtcg ggacaaggtt ttaccggcgg caactggctg gcgtatctgt
     1621 cgctgttcaa tcactacaag cctttcctcg aaggcatttt cgacagcgcc gacgcggcct
     1681 accacatcct gctcatcacc accttcctcg tgctcagcat ccgccgcctg gatgccgacc
     1741 gactcggagg ttgagatgaa ggcggaatca ggaacctgat cgatgtatca cacgccaaaa
     1801 acccactggc aactgcggct gatgaacctc atcttcgtgg tgttgttcct gctggccgtg
     1861 gggctgctgc aatggttggc gaaggaatac cacctgcaat tcgactggac ccgcatgcac
     1921 cggcattcgc tctcgccggc gagcaaagcg gtgacggaac gactcaagga accgctcaag
     1981 atcaccgcct tcgccagcca gcgcggcgag acgcgccggc agatccagga gatggtgggg
     2041 cgttatcaga aacacaaacc cgacatccag ctcgaattcg tcgatccgga caccgcgccc
     2101 gaacgcgtac gcgccgccgg cgtgcagctc gacggcgaac tggtgttcga atacggtgac
     2161 gcgcgcgaga acctgccgcc gacccagttc aacgagcaga gtttcaccaa cgcattgacg
     2221 cgtctcggcc accgcggcga acgctgggtg gtgttcctga gcggccatgg cgagcgcagt
     2281 cccgacaagc aggcgaattt cgatttgtcc acctgggccg cgcagctgcg caagcgcggc
     2341 ttccagacta agacccagtc gcttggcgag cacccgcaga ttccgcagaa tacgacggtg
     2401 ctggtaatcg ccggtccgcg cacgcggttg ctcgcgggcg aggtcaagga gatccagaac
     2461 tatctgaatc gcggcggcaa tctgctgtgg ctgcaagatc cgggtccgct gctcggactg
     2521 gaaccgctgg cggaaaatct cggcatcgaa ttccagccgg gcgtcatcgt ggacccggcg
     2581 tccgaagaca tcaccggcaa tgccgcggcg atcgtggtca caaaatatgg cgcgcatccg
     2641 gtggtgcgcg gttttgccga caacaccgtg ttcccccagg ccggcggcat cagtctgaac
     2701 gctccggaag gctggcaagg ctcggtgctg atggatacac gcccttcg
//
LOCUS       DBNGZE010000341         3256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_117875, whole genome shotgun sequence.
ACCESSION   DBNGZE010000341 DBNGZE010000000
VERSION     DBNGZE010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_117875"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="AC16FV_08820"
     CDS             complement(<1..975)
                     /locus_tag="AC16FV_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR2850194.1"
                     /translation="MTLGKSTRLFIASLLLAASPLAPAAETPTLNPANTAWMLTSSAL
                     VLFMTIPGLALFYGGLVRTKNVLSVLMQCFAITAMVSIVWVLFAYSLCFGDGGSNNSW
                     IGGTAKALMAGIGVNTLKGDIPETVFAMFQMTFAVITPALVAGAFAERMKFSAMLIFS
                     LLWLLLVYVPVCHWVWGDGWLQKMGVMDFAGGTVVHVNAGVASLVAALVLGRRKGFPE
                     TAMPPHNLTMTVAGASMLWVGWFGFNAGSALAANGTAGMAMLVTHIGAAAGALTWMLY
                     RLRLRQMSARINLLYNERLAERTRIARDLHDGLLQSLAGVSLQLHGIAK"
     gene            complement(1007..1345)
                     /locus_tag="AC16FV_08825"
     CDS             complement(1007..1345)
                     /locus_tag="AC16FV_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR2850195.1"
                     /translation="MKLITAIIKPSKLDDVRQALSSLGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKTKIEVAVSSDMVERAIETILQSARTGKIGDGKIFVYDLEQVVRI
                     RTSETGTNAL"
     gene            1577..1930
                     /locus_tag="AC16FV_08830"
     CDS             1577..1930
                     /locus_tag="AC16FV_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850196.1"
                     /translation="MNAPKIQTAIPQRRYQFGEYTGVVLGDIESGDGVKYQYILALVR
                     EGEGRPSFYVTAEKNPRRRAQEGSHRLRVITHGLDEEIGCSDAWSELDAFCAEAFAVA
                     ARVLGLVDEQPVTIA"
     gene            1992..2067
                     /locus_tag="AC16FV_08835"
     ncRNA           1992..2067
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_08835"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            complement(2124..2606)
                     /locus_tag="AC16FV_08840"
     CDS             complement(2124..2606)
                     /locus_tag="AC16FV_08840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR2850197.1"
                     /translation="MKAAQGMVVSMHYTLTDDSGAVLDSSRGGEPLNYLHGHSNIIPG
                     LEKALEGAAAGFKSKVTVAPAEAYGEKNPEAIFEAPREHFPPDMKLEVGARVSADGPN
                     GPITFVIVNLTETGAVLDANHPLAGKTLHFDVEITGVRSATKEEIEHGHVHGEGGHHH
                     "
     gene            2785..3069
                     /locus_tag="AC16FV_08845"
     CDS             2785..3069
                     /locus_tag="AC16FV_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HIR2850198.1"
                     /translation="MNTGKPRVEIEYCSQCRFILRASWMAQELLTTFADELGEVALMP
                     GTGGVFEVRLNGERLWNKKEAGRFPEPKEIKQLIRDRIAPTKDLGHSDRT"
     gene            3077..>3256
                     /locus_tag="AC16FV_08850"
     CDS             3077..>3256
                     /locus_tag="AC16FV_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR2850199.1"
                     /translation="MIRHLSVNVTGQGLHEITDRVEAVVREAGIKEGLCTLFIQHTSA
                     SLLIQENADASAKRDL"
BASE COUNT          604 a          972 c         1047 g          633 t
ORIGIN      
        1 cttcgcgatt ccgtggagtt gcagcgagac gccggctagg ctctgcagca ggccatcgtg
       61 caggtcgcgc gcgatgcggg tgcgctcggc caggcgctcg ttgtaaagca ggttgatgcg
      121 ggccgacatt tggcggaggc ggaggcgata gagcatccag gtgagcgcgc cggccgccgc
      181 gccgatgtgg gtgaccagca tggccatgcc ggcggtgccg ttggccgcca gcgcgctgcc
      241 ggcgttgaaa ccgaaccagc cgacccacag catgctggct cccgccacgg tcatggtgag
      301 gttgtgcggt ggcatggcgg tttcggggaa gcccttgcgc cggccgagca cgagcgccgc
      361 cacaagcgac gctacacccg cattgacatg caccacggta ccgccggcga aatccatgac
      421 gcccattttt tgcagccagc cgtcgcccca gacccagtga cacaccggca cgtagaccag
      481 cagcagccag agcaggctga agatcagcat ggcagagaac ttcatgcgct cggcgaaagc
      541 gccggccacc agcgccggcg tgatgacggc gaacgtcatc tggaacatgg cgaacacggt
      601 ttccggaata tcgcccttga gcgtgttcac gccgatgccg gccatgagcg ccttggccgt
      661 gccgccgatc caggagttgt tggagccacc gtcgccgaag cacaggctgt aggcgaacag
      721 cacccatacg atggatacca tggcggtgat ggcgaagcac tgcatcagga cggataaaac
      781 atttttcgtt cgaaccagtc cgccgtagaa cagggcgagc cccggaatgg tcatgaacag
      841 caccagcgcc gacgaggtca gcatccaggc ggtgttggcc ggattgagcg tgggcgtctc
      901 cgccgccggg gccagcggcg aggcggcaag cagcaacgac gcgatgaaca gtctcgttga
      961 tttaccgagc gtcatgattt tctcctgggt gtgatagttg tttggtttat aaggcgttgg
     1021 tgccggtctc gctggtgcgt atccgtacca cctgctcgag atcgtagacg aaaatttttc
     1081 catcgccgat ttttccggtc ctggccgact gcaaaatggt ttcgatcgcg cgttccacca
     1141 tgtccgacga cacggccacc tcgattttgg tcttgggcag gaaatccacc acgtactccg
     1201 cgccgcggta gagttccgtg tgacccttct ggcgtccgaa gcctttgacc tcggtgactg
     1261 tcatgccctg gactccgaga ctcgacaggg cctggcgcac atcgtccagc ttggagggtt
     1321 tgatgatggc ggtgatgagt ttcatgcgcc tttctccttg gtgagatggt ttttgctcat
     1381 aaccggtcgg cgggtccgtg cacgataccg ccaacccgga agttggcggg catcatacaa
     1441 aagccggttc gttacggaaa tgccccggcg accgcacgag ggggcatgcg ggacgccggt
     1501 cggaatgccg cggcagtctt gtgttataaa taccgcggat cggccgaaac atgcggcagg
     1561 gtcaccgggg gtatacgtga acgcgccgaa aatccagacg gcaattccgc aacgacgcta
     1621 ccagttcggt gagtacaccg gggtggtgct cggcgacatc gaaagcgggg atggcgtgaa
     1681 gtaccagtac atcctggcgc tcgtgcgcga gggtgaaggc cggccttcgt tttacgtgac
     1741 ggcggaaaag aatccgcgcc gccgcgcaca ggaaggttcg catcgcctgc gcgtgattac
     1801 gcacgggctg gatgaggaga tcggatgctc cgacgcctgg tccgaactcg atgccttctg
     1861 cgcggaggcc tttgccgtgg cggctagggt gctgggccta gtggacgagc aaccggtgac
     1921 gatcgcctag aagctgtctc aaaacctgct gcgcgtccgt gatccgggct cgggcggtgc
     1981 tcggaatcct catggactgc cgtgtacact ccggttcctg cgctccgtcc tcacccggct
     2041 gacggccgct cgcgacggtt ttgagatagc ttctaagtcg ctgtccgaaa ttcaccggcc
     2101 ggcggccggt aagctctcat ggctcagtgg tggtggccgc cttcgccgtg cacgtgaccg
     2161 tgctcgattt cctctttggt ggcgctgcga accccggtga tctcgacatc gaaatgcagg
     2221 gttttcccgg ccagcggatg gttggcgtcg agcaccgctc cggtttccgt cagattgacg
     2281 atgacgaagg tgatgggtcc gttcggtccg tcggccgaga ctcgcgcgcc gacctcgagc
     2341 ttcatgtcgg gcggaaagtg ttcgcgcggt gcctcgaaga tggcctccgg atttttctcg
     2401 ccataggctt ccgccggcgc cacggtgacc ttggatttga agccggcggc ggcaccttcg
     2461 agcgcctttt ccagacccgg aatgatgttg ctgtggccgt gcagatagtt gagcggttcg
     2521 ccgccgcggc ttgaatcgag cactgcgccg ctgtcatcgg tcagggtgta atgcatggac
     2581 acaaccatac cctgggctgc cttcattgtg gaatcctcat ggaattgggt gggaagaggt
     2641 cgccactata tcgcagtttg ggcgcggcgc ccaaggcgcg gcgacatcac ccgtcgcgcg
     2701 cggggttttt gccgggcatc ggcgagacag catctccgga ccggcttata ctccctgcac
     2761 attttcgttt cccggtgacc cgtcatgaac acaggcaaac cccgcgtgga aatcgaatac
     2821 tgcagccagt gccgctttat cctgcgcgcc tcctggatgg cccaagagct actgacaact
     2881 ttcgcggacg agcttggcga agtggcgctg atgcccggca ccggcggcgt attcgaagta
     2941 cgactgaacg gagagcgcct gtggaacaag aaagaggccg ggcgctttcc cgagcccaag
     3001 gaaatcaagc agctcatccg cgaccgcatc gcgccgacga aagatctcgg acatagcgac
     3061 cgcacctgat ttattcatga ttcgtcatct ctctgttaac gtcaccgggc agggcctgca
     3121 tgagattacc gatcgcgtcg aagccgtggt gcgcgaggcc ggaataaaag agggcctgtg
     3181 cacgcttttc atacagcata cctcggccag cctgctgatc caggagaacg ccgatgcttc
     3241 cgcgaagcgc gacttg
//
LOCUS       DBNGZE010000342         2087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1229012, whole genome shotgun sequence.
ACCESSION   DBNGZE010000342 DBNGZE010000000
VERSION     DBNGZE010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1229012"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..698)
                     /locus_tag="AC16FV_08855"
     CDS             complement(336..698)
                     /locus_tag="AC16FV_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR2850200.1"
                     /translation="MNIFVGNLSRDVTEEDLRAAFSAFGQVSKVTILKDKFSGEPRGF
                     GFVEMPAKAEAQAAINGMNQKDLKGRALNVNEARPKTDRPGGGGGGGGGRGGWGNRGG
                     GGGGGGGGNRGGGGGRRW"
     gene            complement(946..>2087)
                     /locus_tag="AC16FV_08860"
     CDS             complement(946..>2087)
                     /locus_tag="AC16FV_08860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2850201.1"
                     /translation="SLHRLSTPSTKNSRGPRVLVLTPTRELANQVTAAAIKYGKHMRF
                     RIGSVVGGMPYPPQMRLLSQPLDILVATPGRLLDHMERGRIDYSRLEVLVLDEADRML
                     DMGFIHAVEEIAAATPATRQTLLFSATLEGNIAKLASRLLKNPKRIQIATAQERHEHI
                     EQRLHHVDDGAHKHRLLTHLLDDINMDKAIVFTATKRGADRLAKTLHAKGHPAAALHG
                     DMNQNQRNRAITNLRSGTVRLLVATDVAARGIDVSGITHVINFDLPKSAEDYVHRIGR
                     TGRAGAKGIAISFAAPDNFQQLRDIERYTRQTIPAHVIPGLEPTIRPRQQAPSGHRRG
                     GPSGHNPRRPDGAPRHFKGKNSGNHPRHGQRHGSGGTGRGSKVYG"
BASE COUNT          371 a          623 c          666 g          427 t
ORIGIN      
        1 cgaaaataag ttattgaccc agcataattg taattgaaca gagccggtgc gcgatgagtg
       61 tccccgggac tggatagcga cgcggacgtg ccgggtcaat aacggaaatc ttataatttt
      121 tcggttattg aggcgaccaa gcggcttcga tctgcagcca aagtgaaaat agaccatcgg
      181 ctatattcaa gcgattcatg tcgcctcgat aaccggtctg aggtattccc gcaaccaaca
      241 aaacagcacc ctgccaccgg ataccggtga cagggtgcaa gacatctgaa cttaacggga
      301 aagaaagagg gtgatgatct cgatacagca gcgtattacc agcgacgtcc cccgccacct
      361 ccgcgattgc cgccaccacc gcctccgcca ccaccgccgc ggttacccca gccgccacgc
      421 ccaccaccgc ctccgccacc accaccggga cgatcggtct tgggtcgggc ctcgttcacg
      481 ttgagcgcgc gacctttcag atccttctgg ttcatgccgt tgatggccgc ctgtgcctcg
      541 gccttggccg gcatttccac gaagccgaaa ccgcgcggtt cgccgctgaa cttgtccttg
      601 aggatggtga ccttgctgac ctgcccgaag gcgctaaagg ccgcgcgcag atcttcttcc
      661 gtgacgtcgc gcgacaggtt tcctacgaag atattcatgt ctaactcctg gattgaaagg
      721 gtgaggcgtc gtggaccgtg cttcgctttc ggaaacagtg gcagtgaaag tgcgcggccg
      781 gataggcgaa tgatcgggcg tgaaccagct ccttggtttc agcgtaagga agggtgggtt
      841 tcagtgaccc agcttcttcg gcatccgcga cagataatac gcctgtgttt tgctggcgtc
      901 caagcgcccg gagaatatcg cccgatcatt ggcgtgtcgc gccggctagc cgtagacctt
      961 ggagccgcga ccggtgccac ccgaaccatg gcgctggccg tgtcgaggat ggttcccgct
     1021 gtttttccct ttgaaatggc gtggcgcacc gtccgggcga cgtgggttgt ggcccgatgg
     1081 accgccgcga cggtgacccg agggggcctg ttgtcgcggg cggatagtcg gttccagccc
     1141 gggaatgaca tgggccggaa tcgtctggcg ggtgtaacgt tcgatatcgc gcaactgctg
     1201 gaaattatcc ggcgcggcga acgagatggc gatgcccttg gcgccggcac ggccggtgcg
     1261 gccgatgcga tgcacataat cctcggcgct tttcgggagg tcgaagttga tgacatgcgt
     1321 aatgccggag acgtcgatgc cgcgcgcggc cacgtcggtc gccaccagca ggcgcaccgt
     1381 gcctgaacgc aaattggtga tcgcgcggtt gcgctggttc tggttcatgt cgccgtgtag
     1441 cgccgccgcc gggtgtccct tggcgtgcag ggtcttggcc agccggtccg ccccgcgttt
     1501 ggtggcggtg aacacgatgg ccttatccat gttgatgtcg tccagcaggt gcgtcagcag
     1561 gcgatgcttg tgcgcgccgt cgtccacgtg atgcaggcgc tgttcaatat gttcatgccg
     1621 ctcctgcgcc gtcgcgatct ggatgcgttt gggatttttc agcaggcggc tcgccagctt
     1681 ggcgatgttt ccttcgagcg tggcggagaa cagcagcgtc tggcgcgtgg ccggtgtggc
     1741 ggccgcgatt tcttccaccg cgtgaatgaa acccatgtcg agcatgcggt cggcttcatc
     1801 cagcaccagc acttccaggc gcgagtagtc gatgcgtccg cgttccatgt ggtccagcag
     1861 ccgtccgggt gtcgccacca gaatgtccag aggctgggac aacagccgca tctgcggcgg
     1921 atagggcatg ccaccgacga cgctgccgat gcgaaagcgc atgtgcttgc cgtacttgat
     1981 ggctgccgcc gtcacctgat tggcgagttc gcgcgtcggt gtcagcacca gcacgcgcgg
     2041 gccgcgggaa ttcttggtcg acggcgtgct cagccggtgc agcgacg
//
LOCUS       DBNGZE010000343         4185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_812788, whole genome shotgun sequence.
ACCESSION   DBNGZE010000343 DBNGZE010000000
VERSION     DBNGZE010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_812788"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="AC16FV_08865"
     CDS             complement(<1..539)
                     /locus_tag="AC16FV_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850202.1"
                     /translation="MMQGDRRAASARLAEKGLLLFITLLVLVALGIFTATRIVLALYT
                     GMDSVPLSGWPRFLVFGLWFDLATLCYLLVPFLLYEAAFGNRWHPAGWQRWVRYGVFW
                     FVTAALLFNALAELLFWIEFETRFNFIAVDYLVYTREVIGNIRESYPVAPLLLAVAAL
                     ATLLTWALRGPLARAYGVRL"
     gene            complement(746..3079)
                     /locus_tag="AC16FV_08870"
     CDS             complement(746..3079)
                     /locus_tag="AC16FV_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR2850203.1"
                     /translation="MSIDLKEYHAQLDELDPHLRATLEASFAEAARVMTPQGLHNWLE
                     GAKGLSQLGRGNDLVVTYVQAMPAVVKDVGEEVIKDAVMGAMKLASMVSGEVIQLMFD
                     TLPTAANRLGDAELLRGYLGLIHQLSAKVPRGLRPMLGHLDELFGKLTLGGLRRWALW
                     GAQAHLRDFPAQLAYFDLKSADSQAMLQKERRGTLFIDNQRKLNFYLCAFWGRDFFLR
                     PTSGDYETREGYRPFIETRVIHLPDAYDDFAGLPGKDLFRAAAAHAAAHLVYTKSPLS
                     MEQLNPAQMFLISLFEDARVEHLAIREFPGMKQMWAQFHEKVREVSCPTEPVVGLLER
                     LAYALLDENYHDDDGLIQEMAVAFREEFAKRPQDNRLSWDLGVTLYNKLGERGGIPSL
                     RVLESMAIPYRDDNRYIWASDEIAWHEAEYVPAPKQVRKYVTTMDMVNELDCELAGDD
                     AQEIWVLNEPFYLDQEGCTINELEGKEPVSEPYHYQEWDYGVQLHRPNWVTVLERRQK
                     QGDPEEIDRILTEHKPVAARLRHIIDAMQPEGVQRLRHREDGEEIDLNAAIRAMIDIR
                     MGQTPDPRINIRIERKVRDLAVLVLLDLSESTNEKLGDSEKPVIQLAREATSLLSWAI
                     DRVGDPFAIHGFASDGRHDVQYYRFKDFDEHYGDEAKAKLAGMQGGLSTRMGAALRHA
                     GQFLLKQPEQKKLILLVSDGEPADIDVRDPQYLRHDTKKAVEELATRGVRSFCLTLDP
                     NADDYVSRIFGPKGYMVVDHVNRLPERLPMLYAGLTR"
     gene            complement(3084..3392)
                     /locus_tag="AC16FV_08875"
     CDS             complement(3084..3392)
                     /locus_tag="AC16FV_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850204.1"
                     /translation="MDTQHYVGIDEDAFGGMTAIGRVIRDAWIFGLIPETETCRGWDG
                     ARIEQLYARVSETWEPYAHLVSRLPPELRARHARIHDAAMARARTLGWAPDLSEEDDE
                     "
     gene            complement(3394..4185)
                     /locus_tag="AC16FV_08880"
     CDS             complement(3394..4185)
                     /locus_tag="AC16FV_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ domain-containing protein"
                     /protein_id="HIR2850205.1"
                     /translation="MAQYLVKQEPYYRPVSNEVALYEAAYSARMPVMLKGPTGCGKSR
                     FVEHMAWKLKKPLITVACNEDMTASDLVGRYLLDASGTKWHDGPLTVAARIGAICYLD
                     EVVEARQDTTVVIHPLTDHRRQLPLDKKGEMVNCHPDFQLVISYNPGYQSLMKDLKQS
                     TKQRFGAIDFDYPEAEVEAEIVMHETKVDKATAEKLVQIAHRARNLKGHGLDEGISTR
                     LLAYAGQLIARGIEPRAACQMTLVRPITDDPDMRETLDAAVATYF"
BASE COUNT          757 a         1323 c         1304 g          801 t
ORIGIN      
        1 aggcgcacac cgtatgcgcg cgccagcggg ccgcgcaacg cccaagtcag cagggtggcc
       61 agcgccgcca cggccagcag caaaggcgcg acgggatagg attcgcggat gttgccgatc
      121 acctcgcgcg tgtacacgag ataatccacc gcgatgaaat tgaagcgggt ttcgaactcg
      181 atccagaaca gcaattcggc cagtgcgttg aacaacagcg ccgccgtaac aaaccagaaa
      241 accccgtagc gcacccagcg ctgccagccg gccgggtgcc agcggtttcc gaacgccgcc
      301 tcgtacaata ggaacggcac cagcaggtaa cacagagtgg ccaggtcgaa ccacaatcca
      361 aagacgagga agcgcggcca gccggacaac gggacactgt ccatgccggt atacagcgcc
      421 agcacgatcc gggtggcggt aaagataccg agcgcaacca gcaccagcag cgtgatgaac
      481 agcagcagtc ccttttcagc cagccttgcg gacgccgccc gccggtctcc ttgcatcatc
      541 ttattgtctc caaaacattg aacttcgctg cccaagcgcg atcggcagaa acacaattat
      601 ccacgaatcg ggggaaaccc ggcatgagaa tcaggcgata ctgctcatga atccggtaat
      661 ccggccactg cggtatgccg ctaacgcaca agttcccgca tgcccacgca acttcccttg
      721 cggcatagca ccgcagtcaa tcccgctagc gcgtcaggcc ggcgtagagc atcggcaacc
      781 gctccggcag gcggttgacg tggtccacca ccatgtaacc cttcggtccg aagatgcgtg
      841 acacgtagtc atccgcgttg ggatcgagtg tcaggcagaa gctgcgcacg ccgcgggtgg
      901 cgagttcttc cacggccttc ttggtgtcgt ggcgcagata ctgcgggtca cgcacgtcga
      961 tgtcggccgg ctcgccgtcg gagacgagca ggatgagttt tttctgctcc ggctgtttca
     1021 gcaggaactg gccggcgtga cgcagtgccg cgcccatgcg ggtggacagc ccgccctgca
     1081 tgccggcgag tttcgccttg gcctcgtcgc cgtagtgttc gtcaaagtcc ttgaaacggt
     1141 aatactgcac gtcatggcgg ccgtcgctgg cgaagccgtg gatggcgaac gggtcgccga
     1201 cacggtcgat ggcccaggac agcaatgagg tcgcctcgcg cgccagctgg atcaccggtt
     1261 tctctgaatc gccgagcttt tcgttggtgg actccgacag gtcgagcagc accagcaccg
     1321 cgagatcgcg caccttgcgc tcgatgcgga tgtttatgcg cgggtccggc gtctggccca
     1381 tgcggatgtc gatcattgcg cggatcgcgg cgttgagatc gatctcctcg ccgtcctcgc
     1441 ggtggcgcaa gcgctgcacg ccctcgggct gcatggcgtc gatgatgtgc cgcaggcgcg
     1501 cggccactgg cttatgctcg gtgagtatcc ggtcgatctc ctccgggtcg ccctgcttct
     1561 ggcgccgctc cagcaccgtc acccaattgg gccggtgcag ctgcacaccg taatcccatt
     1621 cctggtagtg atagggttcg ctgacgggtt cttttccttc cagctcattg atcgtgcagc
     1681 cttcctggtc gagatagaac ggttcgttga gtacccagat ttcctgggca tcgtcaccgg
     1741 ccagctcgca atcgagttcg ttcaccatat ccatggtcgt aacgtatttg cgtacctgct
     1801 tgggcgccgg gacgtattcg gcctcgtgcc aggcgatttc gtccgaggcc cagatatagc
     1861 gattgtcgtc gcggtacggg atcgccatgg attccagcac acgcagactc ggaatgccgc
     1921 cgcgttcgcc gagcttgttg tacagcgtga cgccgagatc ccagctgaga cggttgtctt
     1981 gcggacgttt ggcaaactct tcacggaacg cgaccgccat ttcctgaatc aggccgtcgt
     2041 catcgtgata gttctcgtcc agcagcgcgt aagcgaggcg ttccagcaag cccacgacag
     2101 gttccgtggg gcaactcact tcgcgcacct tttcatgaaa ctgcgcccac atctgcttca
     2161 tacccggaaa ctcgcggatc gccagatgct caacgcgcgc gtcctcgaac aggctgatca
     2221 ggaacatctg cgcggggttg agttgctcca tggaaagtgg gctcttggtg taaaccagat
     2281 gcgccgccgc atgcgccgcg gcggcacgaa acaggtcctt gcccggcagg ccggcaaagt
     2341 catcgtaggc atccggcagg tgaatcacgc gcgtttcgat gaaaggccgg taaccctcgc
     2401 gcgtttcata gtcgccggag gtcgggcgca ggaagaaatc ccggccccag aaggcgcaca
     2461 gataaaaatt aagcttgcgc tggttgtcga taaagagtgt cccgcgccgt tccttttgca
     2521 gcatcgcctg cgagtcggcg ctcttgagat cgaagtacgc gagctgcgcc gggaaatcgc
     2581 gcaggtgcgc ctgcgcgccc cacaacgccc agcgccgcag gccgccgagc gtgagcttgc
     2641 cgaacagctc gtcgaggtgt cccagcatcg gccgcaggcc gcgcgggacc ttcgcggaaa
     2701 gctggtgaat caggccgaga taaccgcgca acagctcggc atcaccgagg cggttggcgg
     2761 cggtcggcag ggtgtcgaac atgagttgga tcacctcgcc gctcaccatc gaagcgagtt
     2821 tcatggcgcc catcacggcg tccttgatga cttcctcacc cacatccttc acgaccgccg
     2881 gcattgcctg aacgtaggtg acgaccagat cgttgccgcg cccgagttgc gatagtccct
     2941 tcgcgccttc cagccagttg tgcaggccct ggggcgtcat gacgcgcgcc gcttcggcga
     3001 agctggcttc gagcgtggcc cgcaggtgcg ggtccagctc gtcgagctgt gcgtggtatt
     3061 ccttcaggtc aatggacacg gccttactcg tcgtcttctt cggacaggtc gggcgcccag
     3121 cccagcgttc gcgcgcgcgc catggccgcg tcgtgaatgc gtgcgtgccg agcgcgcagt
     3181 tccggcggca gacgactaac cagatgggcg tagggctccc atgtttcgct cacccgtgcg
     3241 tacaactgct cgatgcgcgc gccgtcccag ccgcgacacg tctcggtctc gggaatcagg
     3301 ccgaaaatcc atgcatcgcg aatgacgcgc ccgatcgctg tcatgccgcc gaaggcgtct
     3361 tcgtcaatgc cgacgtagtg ctgggtgtcc atctcagaaa taggtcgcga ccgcggcgtc
     3421 cagcgtttcg cgcatgtccg ggtcgtcggt gatcgggcgc accagcgtca tctggcaagc
     3481 ggcacgcggc tcgatgccgc gcgcgatgag ctggccggcg taggccagca ggcgcgtgga
     3541 gatgccctcg tcgaggccgt ggcccttcaa gttgcgcgcg cggtgcgcga tctgcaccag
     3601 tttctcggcg gtggccttgt ccactttggt ttcgtgcatg acgatctccg cttccacctc
     3661 ggcctcgggg taatcgaagt cgatggcgcc gaagcgttgc ttggtggact gcttcaagtc
     3721 cttcatcagg ctctggtaac cagggttgta ggagatcacc agctggaagt cgggatggca
     3781 gttgaccatc tcgcccttct tgtcgagcgg cagctggcgg cgatgatcag tgagcgggtg
     3841 gatcaccacg gtggtgtcct ggcgcgcttc gacgacttca tcgaggtagc agatcgcacc
     3901 gatgcgcgcg gccacggtga gtgggccgtc gtgccacttg gtgccactcg catccagcag
     3961 ataacggccg acgaggtccg aggccgtcat gtcctcgttg caggcgactg tgatcagcgg
     4021 ctttttgagt ttccacgcca tgtgctcgac gaaccgcgac ttgccacagc cagtcgggcc
     4081 cttgagcatc accggcatgc gcgcgctgta ggcggcttcg tatagtgcca cctcgttcga
     4141 caccggccgg taatacggct cctgttttac caggtactgg gcgat
//
LOCUS       DBNGZE010000344         3322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_327526, whole genome shotgun sequence.
ACCESSION   DBNGZE010000344 DBNGZE010000000
VERSION     DBNGZE010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_327526"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="rlmD"
                     /locus_tag="AC16FV_08885"
     CDS             complement(<1..1016)
                     /gene="rlmD"
                     /locus_tag="AC16FV_08885"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR2850206.1"
                     /translation="MAVPAMKKPAPHAESLSGDETPVATVTAFSHDGRGVARLDGKAV
                     FIEGALPGETVRFRYLERRKRYDTGQLAEILDPSPDRVTPPCPHYGSCGGCDLQHLRP
                     EVQLQAKQQILAEQLERLGKVKPESWLAPITGPALGYRRRARLGVRFVPSEGGVIIGF
                     RRKNKSFLANLETCLVLEPRVSALLPALHVLIAELSCPDRIPQIEVAVGSNAAALVFR
                     HLVPLTEDDRERLAAFGEHNDIRIYLQPARPDSIVALWPADPEPLFYRIPAFDVEIRF
                     EPADFIQINGAVNNATVEQAVNLLEPAPTDSVLDLFSGLGNFTLPLARRAGRVLGVEG
                     EAGLV"
     gene            complement(1028..1798)
                     /locus_tag="AC16FV_08890"
     CDS             complement(1028..1798)
                     /locus_tag="AC16FV_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR2850207.1"
                     /translation="MNILAFDIETIPDVATGRKLYGLDGLSDKETAQAMFAKRREQTG
                     ESEFLRHHMHRVVAISAVLRREDILRVWSLGEPDSSEADLIRRFFDGIEKYTPTLVSW
                     NGGGFDLPVLHYRMLHHGISAPRYWENGSDDQSFRWNNYLSRYHERHTDLMDVLSGYQ
                     PRATAPLDEMAQLLGFPGKLGRHGSEVWDQFLAGKIGEIRDYCETDALNTYLIYLRYE
                     LMRGNLDDAGWKRETDLVRKILREANKPHLNEFLQAWK"
     gene            complement(1800..2684)
                     /gene="cysM"
                     /locus_tag="AC16FV_08895"
     CDS             complement(1800..2684)
                     /gene="cysM"
                     /locus_tag="AC16FV_08895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HIR2850208.1"
                     /translation="MKLLEDFVGNTPLVRLQRLPGKTTNVILAKLEGNNPAGSVKDRP
                     ALSMIKHAEARGEIKPGDTLIEATSGNTGIALAMAAAMKGYKMVLIMPEHMSKERRAV
                     MKAFGAEIIQVSQASGMEGARDLAHQLQTQLKGKVLDQFANPDNPRAHYEGTGPEIWR
                     DTEGKVTHFVSSMGTTGTIMGVSRFLKEKNSRIQIVGVQPSEGSQIPGIRAWRKEYLP
                     KIYDAKRVDRVMEVTQQEAEDMTRHLAHEEGIFCGISSGGATHAALRLSRELNNAVIV
                     TIICDRGDRYLSTGVFPD"
     gene            complement(2758..>3322)
                     /locus_tag="AC16FV_08900"
     CDS             complement(2758..>3322)
                     /locus_tag="AC16FV_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642175.1"
                     /note="5'-3' single-stranded-DNA-specific exonuclease;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR2850209.1"
                     /translation="VPGLHIRDTLDAVAARHPHLLSKFGGHAMAAGLTLERQHFDAFR
                     EAFAAEVSQHLSDDDLQGRVISDGELTPEDMSLPVAELLREAGPWGQGFPEPLFDGCF
                     EIVSQRVVGEKHLKLGLRLPGSTKTMDAIAFNSVRDGKPLAWARIRAAFKLDVNEYQG
                     YRSLQLILEHLEQADAAVPATEPVTTD"
BASE COUNT          557 a         1007 c         1104 g          654 t
ORIGIN      
        1 accaggcccg cttcgccctc cacgcccagc acgcgcccgg cccggcgcgc cagcggcagc
       61 gtgaagttgc ccaagccgct gaaaagatcg agcacgctgt cggtcggcgc gggttcgagc
      121 aggtttaccg cctgctccac cgtggcgtta ttcacggcgc cgttgatctg gatgaagtcg
      181 gccggctcga agcggatttc cacgtcgaag gccggaatcc ggtagaacag cggctcggga
      241 tcggccggcc acagggctac gatggaatcc ggccgcgccg gctggaggta aattcggatg
      301 tcattgtgtt cgccaaaggc ggcgagcctt tcccggtcat cttctgtcag cgggaccagg
      361 tgccggaaca ccagtgcggc ggcatttgaa ccgactgcga cctcgatctg gggaatgcgg
      421 tcagggcagg acagctcggc gatcagtaca tgcagcgccg gcagcagcgc ggaaaccctc
      481 ggttcgagta caagacaagt ctcaagattc gccaggaaac ttttgttttt ccgacgaaaa
      541 cctatgatga ctccgccttc gctaggaacg aagcgcacgc cgagacgcgc gcgccggcga
      601 tagcccagcg ccgggccggt gatgggcgcg agccaggatt cgggcttcac cttgccgaga
      661 cgttcgagct gctcggccag gatctgctgc ttggcctgaa gctggacttc cgggcgcagg
      721 tgctggaggt cgcagccgcc gcagctgccg tagtgcgggc atggcggcgt gactcggtcc
      781 ggactcggat cgaggatttc ggccagttgg ccggtgtcgt agcgcttgcg ccgttccaga
      841 tagcggaaac gcacggtctc ccccggcagc gcgccctcga tgaatacggc cttgccgtcg
      901 agacgcgcca cgccgcggcc gtcgtggctg aaggcagtca ccgtggcgac aggggtttca
      961 tcgccagata gcgattcagc gtggggagcg ggctttttca tcgcgggcac ggccatgtgc
     1021 acggcgttta tttccaggcc tggaggaatt cgttcaggtg cggcttgtta gcctcgcgca
     1081 ggattttgcg caccaagtcg gtttcgcgct tccagccggc gtcgtcgagg ttgccgcgca
     1141 tgagttcgta gcgcaggtaa atgagatagg tattgagcgc gtcggtctcg caatagtcac
     1201 ggatctcgcc gatcttgccc gccagaaact ggtcccacac ctcgctgccg tggcggccga
     1261 gcttgccggg gaagccgagc agctgcgcca tctcgtcgag tggcgcggtg gcgcgcggct
     1321 ggtagccgga tagcacgtcc atcaagtcgg tatgacgctc gtggtaacga ctgaggtaat
     1381 tgttccagcg gaagctttgg tcgtcgctgc cgttttccca gtagcgcggc gcggaaatgc
     1441 cgtgatgcaa catgcggtaa tgcagcaccg gcagatcgaa accgccgccg ttccagctga
     1501 ccagcgtggg cgtgtatttc tcgatgccgt cgaaaaagcg gcggatcaga tcagcctcgc
     1561 tcgaatccgg ttcgcccagc gaccacacgc gcagaatatc ttcgcgccgc aacaccgccg
     1621 agatcgccac cacgcgatgc atgtgatggc gcaggaattc ggattcgccg gtctgctcgc
     1681 ggcgcttggc gaacatcgcc tgcgcggttt ccttgtcgct cagcccgtcg aggccataca
     1741 gcttgcggcc ggtggcgacg tcggggatgg tttcgatgtc gaaagcgagg atgttcatct
     1801 caatctggga atacgccggt cgagagataa cggtcgccgc ggtcgcagat gatggtgacg
     1861 atcacggcgt tgttcagttc ccgtgacaga cgcagcgccg cgtgcgtcgc gccgcccgaa
     1921 gagatgccgc agaaaatgcc ctcctcgtgt gccaggtggc gcgtcatgtc ttcggcttcc
     1981 tgctgcgtaa cttccatgac tcggtccacg cgtttggcgt cgtagatttt cggcagatat
     2041 tctttccgcc aggcgcggat gccgggaatc tgcgaacctt cggacggttg tacgccgacg
     2101 atttgaatcc gggaattttt ttccttcaga aagcgtgaca cacccatgat ggtgccggtg
     2161 gtgcccatgg agctgacgaa gtgcgtgacc ttgccttcgg tgtcgcgcca gatttcggga
     2221 ccggtgccct cgtagtgcgc gcgcggatta tcggggttgg cgaattgatc gagcaccttg
     2281 cccttgagtt gtgtctgcaa ctggtgcgcc aggtcgcgcg cgccttccat gccggaagcc
     2341 tgtgatacct ggatgatttc cgcgccgaag gccttcatca cggcgcgtcg ttccttgctc
     2401 atgtgctcgg gcatgatcag gaccatcttg tagcccttca tggccgcggc catggcgagc
     2461 gcgatgccgg tgttgccgct ggtggcctcg atcagcgtgt cgccgggctt gatctcgccg
     2521 cgcgcctcgg cgtgcttgat catggacagc gccggccggt ccttgaccga gccggcggga
     2581 ttgttgccct ccagcttggc gagaatgacg ttcgtggtct tgcccggcag ccgttgcagg
     2641 cgtaccagcg gcgtgttgcc gacaaaatct tccagaagtt tcatggacgg aatattaacc
     2701 tgcgtgcgcc cgcaatgcga cggcagccgg gacatcaaac atgcgccgcg gggtcattca
     2761 gtcggtggtc acgggttcag ttgcggggac ggcggcatcc gcctgttcca ggtgttcgag
     2821 gatcaactgc aaggagcggt acccctggta ttcgttcacg tcgagtttga aggcggcgcg
     2881 gatgcgcgcc caggccaatg gcttgccgtc acgcacgctg ttgaaggcga tggcgtccat
     2941 ggtcttggtg ctgccgggca ggcgcagccc gagcttcagg tgtttctcgc cgaccacgcg
     3001 ctggctcacg atctcgaagc agccgtcgaa cagcggttcc gggaaaccct gcccccaagg
     3061 gccggcctcg cgtaacagtt cggcgaccgg cagcgacatg tcctcaggcg tcagctcgcc
     3121 atcgctgatg accctgcctt gcaggtcatc gtcggacaga tgctggctga cttccgcggc
     3181 gaaggcctcg cggaaggcat cgaagtgttg ccgttccagc gtcaggccgg cggccatggc
     3241 atgcccgccg aacttgctga gcaggtgcgg gtggcgcgcg gcgacggcat cgagcgtgtc
     3301 gcgaatatgc agaccgggca cg
//
LOCUS       DBNGZE010000345         1893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1577038, whole genome shotgun sequence.
ACCESSION   DBNGZE010000345 DBNGZE010000000
VERSION     DBNGZE010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1577038"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="AC16FV_08905"
     CDS             <1..383
                     /locus_tag="AC16FV_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HIR2850210.1"
                     /translation="LRDMGQALFDRFCQDMDHNLREMGVGDLSVPKEMRRMGEAFYGR
                     TRAYRAALAAQGEEPLIEAMACNVYGGEPPLSAAPARLAAYMREAVRQIRGHQSAKLL
                     AGDLRFPDPDSVVLVEDKGHLSED"
     gene            343..918
                     /locus_tag="AC16FV_08910"
     CDS             343..918
                     /locus_tag="AC16FV_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR2850211.1"
                     /translation="MFLLKIKVTYQKTESMDKTQNPWSVLVDVEDIPDAGLHVEIDAP
                     EAVRAALRALTNLRELPRLSAVFDLARQGAGVRVAGRVSARVGQTCVVTLEPVESDLE
                     ESVDLVFAPALAESAKAGTVELGTQDEDSPEPLVGGKLDLGAIATEFLLLGIDPYPRK
                     AGAEFAPVKADDGGARPFAALEALKKRLGSG"
     gene            1059..>1893
                     /gene="plsX"
                     /locus_tag="AC16FV_08915"
     CDS             1059..>1893
                     /gene="plsX"
                     /locus_tag="AC16FV_08915"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR2850212.1"
                     /translation="MPDKVRIALDVMGGDHGAAVILPGADISLTRHPDIEYLLFGNRA
                     TLEPVLDGLPRLKARSKLVHTDVAIRMDDKPSQALRYGRWKSSMWLAIDAVKKGEADA
                     AVSAGNTGALMAMARFNLKMIEGIERPAIAALWPTLKGESIVLDLGASIGADEMHLVN
                     LAAMGSAMARVLFDVERPTVGLLNIGVEEVKGLEQVREAGRILREGQFPHFDYHGFVE
                     GDDIGKGVVDVVVTEGFSGNIALKTAEGTAHQLASYLRAAMNRTWRARLGYLLARQAF
                     RT"
BASE COUNT          379 a          522 c          628 g          364 t
ORIGIN      
        1 cattgcggga catgggccag gccctttttg accggttttg ccaggacatg gaccacaacc
       61 tgcgggaaat gggcgtaggc gatctctctg tgccgaaaga gatgcggcgt atgggcgagg
      121 cgttctatgg tcggacgcgt gcctaccgag ccgcgctggc ggcacagggt gaggaaccgc
      181 tgatcgaggc tatggcgtgt aatgtctatg gcggcgaacc accgctatcg gctgcgccgg
      241 cacggcttgc agcctatatg cgggaagcgg tacgccaaat taggggccac caatcggcta
      301 aactgctggc gggcgatctg cggttcccgg acccggactc tgttgttctt gttgaagata
      361 aaggtcactt atcagaagac tgaatctatg gataaaaccc aaaatccatg gagcgtgctg
      421 gtggatgtgg aagatattcc ggacgccggc ctgcatgtcg aaatcgatgc acccgaagcc
      481 gtgcgcgccg cgctgagggc gctcaccaac ttgcgcgaat tgccacgcct ctctgcggtc
      541 ttcgatttgg cacgccaggg tgcgggtgtg cgcgtagccg gccgggtcag cgcgcgggtc
      601 gggcagacat gtgttgtcac gcttgaaccg gtcgaaagcg atctcgaaga gtcagttgat
      661 ctggtatttg cgcccgcgtt ggcggaatcg gcgaaggccg gaacggtcga gctcgggaca
      721 caggacgagg actcaccgga accgctcgtc ggtggcaaat tggacctggg tgcaatcgcc
      781 acggaattcc tgcttctcgg cattgatccc tatccacgaa aggcgggcgc cgaattcgcg
      841 ccggtcaagg cagacgatgg cggcgcacgg ccgttcgcgg ccctggaagc gctaaaaaag
      901 cgcctcggca gcggttagtc agaagcgggc gggccagggg atgagaagtg cggagaaccg
      961 gcttgtctgc tgcgaccgag cggctatggt cgcgcccgtg cgccgaattc aaatgaccgc
     1021 gctgccggcg cccttgttaa tgccgccaag agcgatccat gccggataag gttcgcatcg
     1081 cgcttgatgt tatgggcggc gaccatggcg cggcggtgat tttgccgggt gccgacattt
     1141 cgctcacgcg ccacccagac attgaatacc tactcttcgg caacagagcg acgctggagc
     1201 cggtgctcga tgggctgccc cgtctgaagg cacggtcgaa actcgtgcac accgacgtag
     1261 cgatccgcat ggacgacaag ccaagtcagg cgctgcgtta cgggcgctgg aagtcgtcaa
     1321 tgtggctcgc catcgatgcg gtgaagaaag gtgaagcaga tgccgcggtc tccgccggca
     1381 acaccggcgc gttgatggca atggcgcgct ttaatctcaa gatgatcgaa ggcatcgagc
     1441 ggcctgcaat tgccgcgttg tggccgacgc tcaagggcga atccatagtg ctcgatcttg
     1501 gcgcttcgat cggtgcagat gaaatgcatc tggtcaatct tgcggccatg ggtagcgcga
     1561 tggcgagagt gctatttgat gttgagcggc cgacagtcgg cctgctcaac atcggtgttg
     1621 aggaagtcaa aggacttgag caggtgcgtg aagcaggccg catcctgcgc gaaggtcaat
     1681 tcccgcattt cgactaccat ggcttcgtcg aaggcgatga catcggcaaa ggggtcgtcg
     1741 acgtggttgt caccgagggt ttctcaggga acatcgcgtt aaaaacagcg gaaggcacgg
     1801 cccatcaact cgcttcttat ctgcgcgcag cgatgaaccg aacctggagg gcgcgtcttg
     1861 gatatctgct tgcccggcaa gccttccgga cgc
//
LOCUS       DBNGZE010000346         4669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1855041, whole genome shotgun sequence.
ACCESSION   DBNGZE010000346 DBNGZE010000000
VERSION     DBNGZE010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1855041"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="AC16FV_08920"
     CDS             complement(<1..1106)
                     /locus_tag="AC16FV_08920"
                     /inference="COORDINATES: protein motif:HMM:NF023169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HIR2850213.1"
                     /translation="MRTITATAGRCLALLAAIVGMTATAWGAEIQSIAWEPGSETSVL
                     QVRAAGEGSFTTQVLEDGQRLRISFPNSSMGPSLAELQGQDKVKGVYPYLADNGTAVV
                     VDLLMNEPGQLDVQKAEYGYRVVASSVSAPATPVAATPPPVAVAAPTKVEQPAPEKPA
                     TTEATPAAPAVEEKNSIEDIVYTKLPGDRIQITFKMTKAPAEPNAFTITNPARISLDF
                     PNTRVGLEKKSLAIKEAAVTNVTAIEAEDRSRVVLSLIKPVAYSTSIEGNNFVVTVEA
                     PVSAIAGAVEPKTTHFASTRKTGKYSLKAIDFRRGPQGDGKIVINLSDPAVGIDIREQ
                     AGEIILDFLNTSASAELQRRLDVVDFATPVQTIDV"
     gene            complement(1195..1689)
                     /locus_tag="AC16FV_08925"
     CDS             complement(1195..1689)
                     /locus_tag="AC16FV_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR2850214.1"
                     /translation="MAGLFCAMLSGCSSDGLEDLREFVNNAYADKKPKVEPLPEIKTQ
                     ETFIYNAANLADPFASFNLMPQGQKSASGPRPDPNRRKEPLEDYPLDSLKMVGTLMRG
                     KQAWAVIQAPDGTVHRAQVGDHLGQNSGMINKITEEKIDLIELIQGTMGDWVEREANM
                     TLLE"
     gene            complement(1616..2341)
                     /locus_tag="AC16FV_08930"
     CDS             complement(1616..2341)
                     /locus_tag="AC16FV_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR2850215.1"
                     /translation="MTLDDLKNIDVNNLSSWPVPIKIGAILVVCIIILFAGFWFLIQG
                     ELEEYGAAQKKEEGLRETFMNKKGLAINLPAYKQQMEEMEQTFGSLLRQLPNTTEVPD
                     LLVDITQAGLGRGLEFALFRPEKEQPKDFYAEMPISVEVRGSYHELALFVSDVAALPR
                     IVTFGDINIVASGKNNKLTMSAKARHTAISRKVAPRKRPRQNPGGGRVNHETGNTVTD
                     GGPVLRDAERLQQRWPGRFARVR"
     gene            complement(2338..2913)
                     /locus_tag="AC16FV_08935"
     CDS             complement(2338..2913)
                     /locus_tag="AC16FV_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR2850216.1"
                     /translation="MTTRINLLPWREMQRKEQDRQLLTIAVGAWILMAVIIFYAHVHV
                     TALIENQTRRNDFLNQEIVKVEKEIKEIAELKKKRADLIARMNVIYQLQGDRTQVVHL
                     FDELARKLPEGVYLASLKHSGSNIALQGVAQSNARVSALMRNLAASDWFAEPELEVIT
                     VKAKGSDRVSEFSLKVKQVAKQLKKLEDTGT"
     gene            complement(2910..3986)
                     /locus_tag="AC16FV_08940"
     CDS             complement(2910..3986)
                     /locus_tag="AC16FV_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR2850217.1"
                     /translation="MNALTGLFSKKKPPLIGIDISSSAVKVLELSRNGEHLRVERYAV
                     EPLPQNAVVEHAITEVEQVAQVVERAVKKSGTKLKHAAVAVPAAHVITKTVKMAANLS
                     DQDRKTQVEMEADHYIPYPLNEINLDFQVLGPSESNPEEVDVLMAACRKEIVDDYLAV
                     IQGPGLTPLVVDVETYAMENAFSLITSHMPGGGMEKTIAVLDIGATATTINVVHNNRS
                     VYTRDHAFGGRQLTEEIQRRYGLSYEEAGLAKKQGGLPDNYQTDVLRPFMEAMCQEIM
                     RALQFFYSSTPFNSVDQLLLAGGCGQIAGIDELVAARIGVPAMVANPFASMSLASRIK
                     PQPLSNDAPSLMISCGLAMRSFDE"
     gene            4233..>4669
                     /locus_tag="AC16FV_08945"
     CDS             4233..>4669
                     /locus_tag="AC16FV_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR2850218.1"
                     /translation="MNDSGSSFSRWTRQHLPTQRWQLVLIGLFGLFASGVVILTLIAL
                     ILTPTLPSLDELSGAQLKVPMRVYTADGELIAEFGEEKRIPVKIEEVPDLLIKAVLAA
                     EDRSFFYHHGVDFVGILRAALHNLRTQSTGQGASTITMQVARN"
BASE COUNT          890 a         1392 c         1309 g         1078 t
ORIGIN      
        1 acgtcgatgg tctgcaccgg cgtggcgaag tccaccacat cgagacggcg ctgcagctcg
       61 gcggacgccg aggtattcag aaagtccagt atgatctcgc cggcctgctc gcggatgtcg
      121 atacctacgg cggggtcgga caggttgatg acgatcttgc cgtcgccttg cgggccgcgt
      181 cggaaatcaa tggccttgag gctgtacttg ccggtcttgc gggtgctggc aaaatgcgtg
      241 gtcttcggtt ccaccgctcc cgcgatagcg ctgacgggcg cctccacggt tacgacgaaa
      301 ttgttgcctt cgatgctggt ggaatacgcg accggcttga tcaggctcaa caccacacgc
      361 gagcgatctt ccgcttcgat ggcggtcacg ttggtcacag ccgcttcctt gatggcgaga
      421 cttttctttt cgagcccgac acgggtattc ggaaaatcca gcgatatgcg cgccgggttg
      481 gtgatggtaa aggcgttcgg ctcggcaggc gccttcgtca tcttgaaggt aatctgaatc
      541 cggtccccgg gcagttttgt ataaacaatg tcctcgatgg aatttttctc ttccacggcg
      601 ggggcagcgg gtgtcgcttc ggtggtcgcc ggtttttccg gagcgggctg ctcgaccttg
      661 gtcggcgcag ccaccgcaac gggcggtggc gtggcggcaa ccggcgtggc cggcgcggat
      721 acggaagaag ctaccacgcg atatccgtat tctgccttct gcacatcgag ctgaccgggt
      781 tcattcatca gcaaatccac cacgacagcc gtgccgttgt cagcaagata aggatacaca
      841 cctttcacct tgtcctgacc ctgaagctcg gcgagcgatg gacccatgct gctgttcggg
      901 aagctgatgc gcagtcgctg accgtcttcc agcacttgag tggtaaaaga accctcgccg
      961 gccgcgcgca cctgcagcac cgatgtttca ctccccggtt cccaggcgat ggattggatc
     1021 tccgcgcccc aggcggtggc ggtcatcccc acgatggcgg ccaataacgc caagcagcga
     1081 ccggcggtgg ccgtgatagt gcgcatgatt cgtgtgtgct gtttcgtaaa cgtagccctg
     1141 ctcactgcgc tcgctcgttt taccataaaa ttatcctgcc tctgtttgtc cagttcattc
     1201 caacaatgtc atgttggctt cacgttcaac ccaatcgccc atcgttccct gaatcagttc
     1261 gatgaggtcg attttttcct cggtgatctt gttgatcatg ccggaattct gccccagatg
     1321 atcaccgact tgggcgcggt ggacggtccc gtccggcgcc tggataacgg cccacgcctg
     1381 cttgccgcgc atgagggtgc ccaccatttt cagcgagtcc agaggatagt cttcgagagg
     1441 ctccttgcgc cggtttggat ccggccgtgg accgcttgca ctcttctgcc cctgaggcat
     1501 aaggttgaaa gacgcgaaag gatcggccag attcgccgcg ttataaataa acgtttcctg
     1561 tgttttgatt tccggcagtg gttccacctt cggcttcttg tcggcatagg cgttgttaac
     1621 gaactcgcgc aaatcttcca ggccatcgct gctgcaaccg ctcagcatcg cgcaaaacag
     1681 gcccgccatc agtaacggta ttgccggttt catggttaac ccttccgccc ccggggtttt
     1741 gccggggccg ctttcgcggc gccaccttcc tcgagatagc ggtatgtctt gccttggccg
     1801 acatggtcag cttgttattc ttgccgctgg caacaatgtt gatgtcacca aacgttacga
     1861 tacggggaag cgcggcaacg tcgctgacaa acagcgccag ctcgtgatag ctgccacgta
     1921 cctcgacgct gatcggcatc tcggcataga aatccttggg ctgttccttt tccggacgga
     1981 acagcgcgaa ttccagaccg cggcccaagc cagcctgggt gatgtccacc aacagatcgg
     2041 gaacttctgt ggtattgggc aattgccgca gcaggctgcc gaaggtctgc tccatctctt
     2101 ccatctgctg cttgtaggcc ggcagattga tcgccaaacc cttcttgttc ataaaggtct
     2161 cgcgcaggcc ctcttccttc ttttgcgcgg cgccatattc ctccagctca ccctgaatca
     2221 gaaaccagaa accggcgaaa agaatgatga tgcacacaac caaaatggcg ccgatcttga
     2281 ttggcaccgg ccaggaactg agattattga cgtcgatatt tttcaggtcg tccagcgtca
     2341 tgtgccggta tcctcgagct tcttcagctg cttcgcgacc tgtttgacct tgagcgagaa
     2401 ctcactgaca cgatcgctcc ctttggcctt gacggtaatc acttcgagct ccggttcagc
     2461 gaaccagtcc gaagccgcca gattacgcat gagcgccgaa acacgagcat tcgactgggc
     2521 cacgccctgc agggctatgt tgctgccaga gtgcttcaaa gacgccagat agacgccttc
     2581 cggcaatttg cgcgccagct catcgaatag atgtaccacc tgggtacggt ctccctgcag
     2641 ctgatagatc acgttcatgc gcgcaatcag gtccgcgcgc ttctttttca gctcggcaat
     2701 ctccttgatc tctttctcga ccttgacgat ttcctgattc aggaagtcat tgcgccgggt
     2761 ctggttttct atcaatgcag tcacatgcac gtgggcataa aagatgatta cagccatcag
     2821 aatccacgcg cccacggcga tcgtcaggag ctgacgatcc tgctccttgc gctgcatctc
     2881 gcgccaggga agaagattaa tgcgcgtggt cattcgtcga agctccgcat ggcgagcccg
     2941 cagctgatca tgagcgaggg cgcgtcattg gacagaggtt gcggcttgat gcgcgaggcc
     3001 aatgacatgc tcgcgaaggg attggccacc atggccggca cgccgatgcg cgccgccacc
     3061 agctcatcga tgcccgcgat ctgtccgcac ccgcccgcca gcagcaactg atccacgctg
     3121 ttgaacggtg tcgaggagta gaagaactgc aaggcgcgca tgatttcctg gcacatggcc
     3181 tccatgaacg gtcgcagcac gtcggtctgg tagttatccg gcaggccgcc ctgtttcttg
     3241 gccagccccg cctcttcata agagaggccg taacggcgct ggatctcttc cgtgagctgc
     3301 cggccgccga aggcgtggtc gcgggtgtac accgagcgat tgttgtgcac cacgttgatg
     3361 gtggtcgcgg tggcgccgat atctagcacg gcgatggtct tttccatgcc gccgccgggc
     3421 atgtgactgg tgatcagaga gaaggcgttc tccatggcat aggtttcgac gtccacgacc
     3481 aatggcgtca ggcccggccc ctgaatcacg gcgagatagt cgtccacgat ctccttgcgg
     3541 caggcggcca tcagcacgtc cacctcttcc ggattgcttt ccgaagggcc gaggacctgg
     3601 aaatcgagat tgatttcgtt cagcggatag gggatgtaat gatccgcttc catctccacc
     3661 tgggtcttgc ggtcctgatc gctcaggttg gccgccatct tgacggtctt ggtaatgacg
     3721 tgcgccgccg gcacggccac ggcggcgtgc ttgagtttgg tgccggattt cttaacggcg
     3781 cgttccacca cctgcgccac ctgttccact tccgtgatcg catgctcgac cacggcattc
     3841 tgggggaggg gttccaccgc atagcgctca acgcgcagat gctcgccgtt tctgctcagc
     3901 tcgagcacct tgacggcgga agagctgatg tcgatcccta tgagtggcgg cttctttttg
     3961 gaaaataaac ccgtgagggc attcacttta ttttcctctc taaatccttg agttatgatt
     4021 gatacatata aatgtagatc aaatatagac catggaattc agaaatgaca aattgttgaa
     4081 tttgcccgga tttacgggtt tttcagcaga tttttatccg gtgacaacgt cacgcgatcg
     4141 taaataccgc gtttgacagg cagtggttta tactcgctgg cgcgccgggt tccggacgct
     4201 cctgacacaa atttccacaa gccttgccca ctatgaatga ttctgggagt tctttctccc
     4261 gctggacccg ccaacacctg cccactcagc gctggcaact ggtgctgatt ggactctttg
     4321 gcttgttcgc gagcggggtg gtgatactca ccctgatcgc tctcatactc acgcccacgc
     4381 ttccctccct cgatgagctc tcgggggccc aattgaaagt gcccatgcgg gtttataccg
     4441 ccgacggaga actgatcgcc gagttcggcg aagaaaaacg cattcccgtg aaaatcgaag
     4501 aggtgccgga cttgctcatc aaggcggtgc tggccgccga agaccgctcc ttcttttatc
     4561 accacggcgt ggacttcgtc ggcatcctgc gcgccgccct gcataatctg cgcacccagt
     4621 cgaccgggca gggcgccagc accatcacca tgcaggtggc gcgcaactt
//
LOCUS       DBNGZE010000347         1735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_882635, whole genome shotgun sequence.
ACCESSION   DBNGZE010000347 DBNGZE010000000
VERSION     DBNGZE010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_882635"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1735)
                     /locus_tag="AC16FV_08950"
     CDS             complement(<1..>1735)
                     /locus_tag="AC16FV_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850219.1"
                     /translation="GEAATCLERAAELEPGNAGTWIMLGGLQGMLGVFDRAETSNRRA
                     LALQPGSVEARMNLANALLPQGKLDEAATCYETVLRQQPGFAAGWFMLGRTRRRQGQY
                     GEAERCCREAIRLNPALAEAQADLGNILRAQGKASEAVAVFREAATRSAAHVGVLYQI
                     GCALQDLGQFNDAADCFRNALRLKPDLAEAYIRLASVCQAKSDHHEAMANCEQALRIK
                     PDLAEAHHVLAVSFFALDRWDEAVSSFERALHIRSDYVDAHLGLATVMLALGKPDQAR
                     ASCEKALALDPANSDAIALAAYIAKRAGETEKAYRLLYPLVERGVLGANVAVTLATIS
                     KDLGRQSEAIALMEKTLGSDPALSDAARINLHFNLGMLYDDTKQYDRAFFHYQQGNRL
                     KPLSFDRAGFERIVEGIVAIHTPEFMARMPRARVRSDRPVFIVGMVRSGTSLVEQILS
                     SHPDVHGAGELPDITQIARELPGILGSGERYPECMPGLTREIADAQAKRYLDRLSRIS
                     PSARRVTDKLPGNFMYLGLIELLFPGARVIHCRRDPVDTCLSAYFQDFSNNHPYAYDL
                     ANLGVFYRGYRK"
BASE COUNT          329 a          532 c          537 g          337 t
ORIGIN      
        1 tcttccggta accgcgataa aagacgccca ggttggcgag gtcgtaggcg tacgggtggt
       61 tgttggaaaa atcctggaaa taggccgaca ggcatgtatc caccgggtcg cgccggcagt
      121 gaatcacgcg cgcgccggga aacagcagct caatgagacc gagatacatg aagttgccgg
      181 gcaatttgtc ggtcacccgc cgggcgctgg gagaaatccg ggagagacga tccagataac
      241 gttttgcctg ggcatcggcg atctcccggg taagtccggg catgcactcg ggatagcgtt
      301 caccggaacc cagaatgccg ggcagctccc tcgcgatctg ggtgatatcc ggcaattcgc
      361 cggccccatg gacgtccgga tggctggaga ggatttgttc gaccaagctg gtgccggaac
      421 gcaccatgcc gacgatgaag accggtcgat ccgatcgaac gcgggcgcgc ggcatgcgtg
      481 ccatgaattc cggcgtatgg atggccacga tcccttcaac gatgcgctca aagccagccc
      541 tgtcgaagct caatggtttc agcctattgc cttgctgata gtgaaagaag gcccggtcat
      601 attgcttcgt gtcgtcatac agcattccga ggttgaaatg caggttgatt cgagcggcat
      661 cactcagggc agggtcggaa ccgagggttt tttccatcag ggcgatagcc tcggattgcc
      721 ggcccaagtc tttgctgatc gtggccaggg tgaccgccac attggccccc agaacaccgc
      781 gctccacaag ggggtaaagc agccgatagg ccttttcggt ttccccggcg cgcttggcga
      841 tgtaggcggc cagggcgatg gcgtcactat tggcgggatc gagagccaac gccttttcgc
      901 aactcgcgcg ggcctgatcg ggtttgccga gcgccagcat gaccgtggcg agtccaagat
      961 gggcgtctac gtaatcggat ctgatatgca gcgcccgttc gaaactcgat accgcttcgt
     1021 cccatctgtc gagcgcgaaa aatgacacgg ccaggacatg atgtgcttcc gctagatccg
     1081 gcttgatgcg caacgcctgc tcgcaatttg ccatggcttc atgatgatcc gatttggctt
     1141 ggcacaccga cgccagtcgg atatatgcct cggccagatc gggcttgaga cgtaaggcat
     1201 tacggaagca gtcggcggcg tcattgaact gcccgagatc ctgtagtgcg catccgattt
     1261 gataaagcac accgacatgt gccgccgagc gcgtcgcggc ctcccggaag acggccaccg
     1321 cctcggacgc ttttccctgc gcgcgcagga tgttccccaa gtccgcttgc gcctcggcca
     1381 gcgctggatt caggcggatc gcttcgcggc aacagcgctc ggcctccccg tattgtcctt
     1441 gccgtctgcg cgttctgccc agcatgaacc agcccgccgc aaaacccggc tgctgccgca
     1501 acacggtttc gtagcatgtc gcggcctcgt ccagctttcc ctggggcagc agcgcgttgg
     1561 ccaggttcat gcgcgcctcg acggacccgg gctgcaaggc cagcgcccgc cggttgctgg
     1621 tttcggcccg gtcaaaaacc ccgagcattc cttgtaggcc acccagcatg atccaggttc
     1681 cggcattgcc gggctcgagc tcagcagcgc gctcgaggca ggttgccgcc tcgcc
//
LOCUS       DBNGZE010000348         3604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1855308, whole genome shotgun sequence.
ACCESSION   DBNGZE010000348 DBNGZE010000000
VERSION     DBNGZE010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1855308"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="AC16FV_08955"
     CDS             <1..1295
                     /locus_tag="AC16FV_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2850220.1"
                     /translation="RSSRGGDIRLGKTTSNLFRDRQGAAARKLDVRDFNNVLHVDAAN
                     GYVVVEGMTPYAKLVAECLRHNVMPAVVPQLKSITIGGATVGCGIESSSFRYGLVHET
                     VQEMEILLGDGRAVLCTPDNEHRDLFYGFPNSYGTLGYALKLKAKVVPVKKYVKLTHL
                     RHADPGRFFAELARQCERHDVDFVDGVVFGRDDMVITLGQFVEQAPYTSDYTYKNIYY
                     KSLRERPEDFLTTADYLWRWDTDWFWCSKNLLAQNPLVRRLYGRKRLNSVTYTKLMRL
                     NSRWKLTRSIDRLLGFHTESVIQDVEIPIEHAPEFLAFYHDVIRFMPVWVCPTRAWDE
                     QARFDLYRMDPQKLYINFGFWDVIRGRKKLPPGYYNRQIEERVRAMHGMKSLYSDSYF
                     SPEQFWEIYNKPAYDRLRKKYDPNDALKDLYRKCVLKE"
     gene            1611..1799
                     /locus_tag="AC16FV_08960"
     CDS             1611..1799
                     /locus_tag="AC16FV_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850221.1"
                     /translation="MSQTQRAAKMEMGKKIEVEANRESVEVAEAIKALQVQLADLRKI
                     VQAAKTSDTDDVKPGRRQ"
     gene            complement(1891..2028)
                     /locus_tag="AC16FV_08965"
     CDS             complement(1891..2028)
                     /locus_tag="AC16FV_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850222.1"
                     /translation="MLGEGKFAAVFTILGIIAGIYIYGLAREKARPARDTTLASAVSA
                     D"
     gene            complement(2083..2247)
                     /locus_tag="AC16FV_08970"
     CDS             complement(2083..2247)
                     /locus_tag="AC16FV_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850223.1"
                     /translation="MSQYWITDRLLGYSQGYGHLCGLVSRVETFHRGELASSDDWRLW
                     YLLGSPLAAR"
     gene            2550..>3604
                     /locus_tag="AC16FV_08975"
     CDS             2550..>3604
                     /locus_tag="AC16FV_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR2850224.1"
                     /translation="MYLQFFGLHENPFSIPPDPHYLYLSRGHQEALAHLQYGLAEAGG
                     FVQLTGEVGTGKTLLVRALVERLPEGVDVALILYPVLTVTDFIAAICDELRVPHPGEK
                     ASLKQLIDALNAHLLQTHARGRRTVLIIDEAQNLSREVLEQVRLLTNLETTKQKLLQI
                     ILIGQPELAQMLAQQDLRQLAQRITARFHLVALTRKENFEYILHRCRVAGAKTILFDR
                     KAMEQVYAASGGIPRLINVICDRALLGAYTLGKSVVDASIVKRAAMEVGPVARTKRWP
                     VPRVLAIASALVIVMVVAVWQWWPQWTHVFLSGESPASEVTSSVEAKPANANVASVAP
                     TATSAEANNPASPPNAVS"
BASE COUNT          802 a         1023 c         1055 g          724 t
ORIGIN      
        1 tgcgttcgag ccggggcggg gatattcgcc tcgggaagac cacctccaac ctgtttcgtg
       61 accgtcaggg cgcggcggcc cgaaaactgg acgtgcgcga ctttaacaat gttctgcacg
      121 tcgacgccgc gaacggttat gtcgtggtgg aggggatgac accgtacgcc aaacttgtcg
      181 ccgagtgtct caggcacaat gtcatgccgg cggtggtacc ccagctcaag tccatcacca
      241 tcggcggggc gactgtcgga tgcggcattg aatcctcgtc tttccgctac gggctggtgc
      301 atgagacagt gcaggagatg gagatactgt tgggtgatgg gcgcgccgtg ctatgcacgc
      361 cggacaacga acaccgcgac ctgttttacg ggttccccaa ctcctacggc acgctcggtt
      421 atgccctcaa gctcaaggcc aaggtggtgc ccgtgaaaaa gtacgtgaaa ctcacccatc
      481 ttcgccatgc cgaccccgga agattcttcg ctgagctggc acgacagtgc gaacggcacg
      541 acgtcgattt tgtcgatggc gtggtgttcg gccgcgacga catggtcatt acgctgggcc
      601 agtttgtcga gcaagcgcct tacaccagcg attacaccta caaaaacatc tattacaaat
      661 cactgcgcga gcgcccggag gattttctga cgacggctga ttatctgtgg cgttgggaca
      721 cggattggtt ctggtgttcg aagaatctgc tggcgcagaa tccgctggtg cgccggctct
      781 atggacggaa gcggctgaat tcggttacct acacgaagct catgcggctc aacagtcgct
      841 ggaaattgac acgttccatc gacaggctgc tgggttttca caccgagtcc gtgatccagg
      901 atgtcgaaat cccgatagaa catgcgccgg aattcctggc gttctatcac gatgtcatcc
      961 gcttcatgcc ggtatgggtt tgccccaccc gcgcctggga cgagcaagcc agattcgacc
     1021 tttatcgcat ggacccgcaa aagctctata tcaatttcgg attctgggat gtgatacgcg
     1081 gaagaaagaa actgccgccg ggatactata accgtcagat cgaggagagg gtgagggcga
     1141 tgcatggaat gaaatcgctc tattcggatt cctatttttc gcccgaacaa ttctgggaaa
     1201 tttataacaa gcccgcttac gaccgactca ggaaaaagta tgatccaaac gatgcgctca
     1261 aggacctcta tcgcaaatgc gtgctcaagg aatgagaccc ccggtgtggg tccgctccat
     1321 gtcgggtttg atcgctcaga gggatgtttg taaaaaacac acacctgtcg cattcatgca
     1381 taaacatcca ttgggtagac gcagataaga caagcgtgaa gcacgttttg cgattgtcgg
     1441 cctgacatcg gcggtcgtca gcctgcggaa ccaataaccg ggtctatact tgtaattcct
     1501 ccggttttcc gccttaatga atgaacgccg cttgtgtcgt tctttgtgag gcgcagggga
     1561 agtggtcagc gaagctctca tgtcagtcgg atttgcacaa ggaggttcac atgagtcaaa
     1621 cgcaacgcgc agcaaagatg gaaatgggca agaagatcga agttgaagcg aacagggagt
     1681 cagtcgaggt cgccgaagcg atcaaggcct tgcaggtgca gcttgccgat ctgcgcaaga
     1741 tcgtccaggc cgccaagact tcggacactg acgacgtgaa gcccggacgc cgtcaataaa
     1801 cccctccatc tcccgtgtaa tctgtaatcc cttcttccag ggtccaaacc gtcagccagt
     1861 ttttcaaacc ggccgcgtat gctggatgcg tcagtctgcg ctgacggcag acgccaacgt
     1921 cgtgtcgcgc gccggtcgcg ccttctcgcg cgcaagacca tagatataaa tgccggcgat
     1981 gatgcccagg atggtaaaga cggcggcgaa ttttccttca cccagcatga ccggcgccgt
     2041 gccggggcag gaaccgctca ggccccaaac cgacgccgag catcagcgcg ccgccaaggg
     2101 ggagcccagc aggtaccaca gacgccagtc atcggatgac gccaattccc cgcggtgaaa
     2161 cgtttcaacc cgggaaacca gtccgcagag atggccgtaa ccctgtgagt agccgagcag
     2221 gcggtcggtg atccagtatt gggacaaggg caaacaaacc gatggccaag ccgcccagcc
     2281 agcccggcca gaaagtcatg tctgacacgc tgagtattct cccaggaaac aactaaaaga
     2341 ccgtttcgat gacggtcgtg gttgcatcat tagcaggcag acatagggtg cacaaataat
     2401 ggtatcgtat gaggttataa ggtccgacgc caaacatccg aacgatcagc aagtcaggga
     2461 agattgatgg acaagaaata ctccactggg aatatcccga ggcggagaat gcattccatc
     2521 cggaaccggc agccggcgag ggcggggtaa tgtacctcca gttttttggc ctgcatgaaa
     2581 acccgttttc catcccgccc gatccccatt atctttacct gagccgggga catcaggagg
     2641 cgctggcgca tcttcagtat ggactggcgg aggccggtgg tttcgtgcaa ttgaccggcg
     2701 aggtcggcac gggcaagaca ctgctggtgc gcgcgctggt cgagcgcctg cccgaaggcg
     2761 tggacgtcgc gctgattctt tatccggtgc tgaccgtgac cgacttcatc gccgccattt
     2821 gcgatgaact gcgcgtgccg catcccggcg agaaggccag cctcaagcaa ctgatcgacg
     2881 cgctcaacgc ccatctgctg cagacccacg cacgcggccg gcgcaccgtg cttatcatcg
     2941 acgaggcgca gaacctgagc cgcgaagtgc tggagcaggt caggctgctc accaacctcg
     3001 agaccaccaa gcagaagctg ctgcagatca ttctgatcgg ccagccggag ctggcccaga
     3061 tgctggcgca gcaggatttg cgccagttgg cgcagcgcat cacggcgcgt ttccatctgg
     3121 tggcgctcac tcgcaaggaa aacttcgaat atattctcca tcgttgccgg gtcgcgggag
     3181 ccaagacgat attgttcgac agaaaagcca tggagcaggt ctatgccgcg tccggcggta
     3241 tcccgcgatt gatcaatgtg atctgcgatc gtgccttgct cggtgcttac acgctcggta
     3301 aatccgtggt ggatgcgtcc atcgtcaagc gcgcggccat ggaggttgga ccggtcgcgc
     3361 gaaccaaacg gtggccggtt ccgcgcgtgc tggcaatcgc atcggcactg gtcattgtca
     3421 tggtcgtggc agtctggcag tggtggccac agtggactca tgtgttttta tccggggaaa
     3481 gcccggccag cgaggtgact tcctccgtcg aggcgaaacc cgccaatgcc aatgttgcgt
     3541 ctgttgcgcc taccgcgacg tctgccgaag ccaataaccc ggccagtcca cccaatgctg
     3601 tgtc
//
LOCUS       DBNGZE010000349         1564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1507808, whole genome shotgun sequence.
ACCESSION   DBNGZE010000349 DBNGZE010000000
VERSION     DBNGZE010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1507808"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..890)
                     /locus_tag="AC16FV_08980"
     CDS             complement(162..890)
                     /locus_tag="AC16FV_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR2850225.1"
                     /translation="MPAPATFASSAAAFFGGVKSALTSVFFLVLAGTYVGMGALAHDF
                     GFSSWWLALSTVLVWAAPAQVILISALGAGSALIEAALAVSLSAIRLFPMVVALLPLL
                     RGEGTRLRDLLLPAHFTSVSMWVESIRLLPGLPRERRIAFCNGLSVGYMSTAASFGFV
                     GYYLAAELPPLLAGALLFLTPMSFLLSTARNAKAMMDKLALVLGLLLGTILTATHVQL
                     DLMWTGIGGGTLAYVVHRLRETMA"
     gene            complement(1011..>1564)
                     /gene="clpA"
                     /locus_tag="AC16FV_08985"
     CDS             complement(1011..>1564)
                     /gene="clpA"
                     /locus_tag="AC16FV_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166310.1"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR2850226.1"
                     /translation="ASELAKQAYGFTRTKREGDDQEAISKMFAPEFRNRLDATITFAH
                     LSQDVIAKVVEKFVLQLEAQLGDRNVTIELSDEASRWLIANGYDELMGARPMARVIQE
                     HIKKPLADEVLFGHLKAGGHVRVIVDKDEQGRDKLGFQFLDGPVTPKPEKLPAARPKA
                     KRRASPRPKPKRGSVPKVPLAKV"
BASE COUNT          343 a          497 c          465 g          259 t
ORIGIN      
        1 tgcgagtatc gcggtggcga ccgcgcgcga ccacactacg atttcagaat cttcattgag
       61 cccgcgcgcc aataccaggc cgaacaagcg ccagacttca ttgggaagat atccgaccag
      121 gatcagtacc agataaggcg cgaggtcggc ccaactggca ttcatgccat agtctcgcgc
      181 agacgatgaa cgacataggc cagcgtgccg ccgccgatcc cggtccacat cagatcaagt
      241 tgaacgtggg tggcggtcag gattgtccca agtaacagac ctaataccag cgccagtttg
      301 tccatcatcg ctttcgcatt gcgcgcagtc gacaacagga acgacatcgg cgtgaggaac
      361 agcagcgcgc cggcaagcag cggcggcaat tcggctgcca gataatagcc gacgaagccg
      421 aaactcgctg ccgtgctcat gtagccgacc gacaacccgt tgcaaaaagc gatgcggcgc
      481 tcgcgaggca gccccggcaa cagccggatc gattccaccc acatgctgac ggaggtgaaa
      541 tgcgccggta gcaacaaatc gcgcagccgc gtgccctccc cgcgcagcaa cgggagcaac
      601 gcgacgacca tcggaaacag ccggattgca ctcaggctga cagcgagcgc cgcttcgatg
      661 agcgccgagc cggcgccaag cgcggagatt aagataacct gcgccggcgc ggcccagacc
      721 agcacagtgg atagcgctaa ccaccatgac gaaaagccaa agtcatgggc cagcgcgccc
      781 atgccgacgt aggtgcctgc cagcactagg aaaaacaccg acgtaagtgc cgatttcacc
      841 ccgccgaaaa aggcagcggc agaagatgca aatgtcgcag gcgccggcat gggtgccgca
      901 cacctgtctc atagcgctgg cgtgtccgca actgtgtgcg tacgcatgcg cggtccgcaa
      961 aatgcgaagg ccgggcacaa agcccggcct gtctattgtt caacggtaaa ctacaccttt
     1021 gccagcggca ctttcggcac tgagccgcgc ttgggcttgg gccgcggcga agcgcggcgc
     1081 tttgctttcg gacgcgccgc cggcaacttc tccggcttgg gcgtgaccgg accgtcaagg
     1141 aactgaaagc cgagcttgtc acgaccctgc tcatctttgt cgacaatcac gcgcacatgc
     1201 ccacctgcct tcaaatggcc aaacagtact tcgtcggcca gcggcttctt gatgtgctcc
     1261 tgaatcacgc gcgccatcgg ccgggcgccc atcaattcgt cgtaaccgtt ggcgatcagc
     1321 cagcggctcg cctcgtcaga gagctcgatc gtgacattgc ggtcgccaag ctgggcctcg
     1381 agctggagca caaatttctc gactaccttg gcgatcacgt cctgcgagag atgcgcaaaa
     1441 gtgatggtgg cgtcgagccg gttgcggaat tccggcgcga acatcttgct gatcgcttcc
     1501 tggtcatcac cctcgcgctt ggtgcgggtg aagccgtagg cctgcttggc gagctcggag
     1561 gcgc
//
LOCUS       DBNGZE010000350         7632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1507841, whole genome shotgun sequence.
ACCESSION   DBNGZE010000350 DBNGZE010000000
VERSION     DBNGZE010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1507841"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /gene="accB"
                     /locus_tag="AC16FV_08990"
     CDS             <1..269
                     /gene="accB"
                     /locus_tag="AC16FV_08990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR2850227.1"
                     /translation="PAAAPAEQAPAGHAVASPMVGTFYRSSAPGSAPFVDIGSQVKVG
                     DTLCIIEAMKMMNEIESDKAGVVKAILKENGQPVEYGEALFIIE"
     gene            273..1613
                     /gene="accC"
                     /locus_tag="AC16FV_08995"
     CDS             273..1613
                     /gene="accC"
                     /locus_tag="AC16FV_08995"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR2850228.1"
                     /translation="MIEKVVIANRGEIALRIQRACRALGIRTVALHSEADREAKYVKL
                     ADESVCIGPARASDSYLNIPAVISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFIF
                     IGPKAETIRLMGDKISAIKAMKKAGVPCVPGSDGPVTGSDKEIHRMAAQIGYPVLIKA
                     AAGGGGKGMRVVHTEAALLNALTLTKSEAKAAFGNDTVYMEKYLEHPRHIEFQVLADT
                     HGHAIHLGERDCSMQRRHQKVVEESPAPGLPEKLRHKMGERCAEACRKIGYRGAGTFE
                     FLYQDGEFYFIEMNTRIQVEHPVTEMVTGVDIVREQLRIAAGEKLSYRQKDINWRGHA
                     IECRINAEDPRNFIPSPGRITGWHPPGGPGIRVDSHVYTNYFVPPYYDSMIAKLIAFG
                     DTREMALARMRIALSEMIVDGIKTNIPLHQDIMNDAAFIRGGVNIHYLQKKLRL"
     gene            1659..2543
                     /gene="prmA"
                     /locus_tag="AC16FV_09000"
     CDS             1659..2543
                     /gene="prmA"
                     /locus_tag="AC16FV_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR2850229.1"
                     /translation="MPWLKLKLRVTEAHAQPLADAFEEWGAISVTLEDAADQPLFETH
                     WDKNPIWSQVKVTGLFPEHTNTDDILARARELMNLPESPPHEIDLLGDEDWAHSWMAH
                     YKPLEVGHGLWVVPSWCTPPDPKAVNIILDPGLAFGTGDHPTTSLCLAWLSEQTLAGK
                     TLLDYGCGSGILSIAALKLGAHAAYAVDIDAQALEVTRRNAVHNGIRAGLHAMPPSEL
                     PPDFQADIVIANILFGTLIELAAEITARVRPGGWLVLSGLLGSDQAVEVRPHYEPPFA
                     FEQRERQQWVLLAGHKPD"
     gene            2591..3391
                     /locus_tag="AC16FV_09005"
     CDS             2591..3391
                     /locus_tag="AC16FV_09005"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HIR2850230.1"
                     /translation="MYTQCPACLTTFKVTPAQLEARGGMVRCGICSAVFHAEQRLLQV
                     QPETKPAKTAQEAAAAKNDKRRSAVKNRRRSGRRRSDKLQTLDEKLTHDANIPVITKQ
                     PLFTRPRLRVSSLLWGFGSLLLLTLLAGQFVYFYRSELATIPEWRPYLVDICLYAGCE
                     LRPLRDVGRIELLQTSIAPHPQYENALRIRATLVNRAAFRQAYPWMEVSLTSNAGNVI
                     ARRVFAPIQYSETPLEDALTPNVVATALLDVTNPDSKAVGYEIRLVTP"
     gene            3628..4659
                     /gene="dusB"
                     /locus_tag="AC16FV_09010"
     CDS             3628..4659
                     /gene="dusB"
                     /locus_tag="AC16FV_09010"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR2850231.1"
                     /translation="MRIGPYELKNNLFLAPMAGVTDRPFRTLCRKLGAGMAASEMVSS
                     NSLLWGSKKTLRRADHSGEEDPRAVQIVGSDPAMMAQAARYNVDNGAQIIDINMGCPA
                     KKICNVYAGSALMQNEKLIVEILEAVVKSVEVPVTLKIRTGWDRANRNGPAIARIAED
                     SGIQALAVHGRTRADAFQGEAEYDTIAAIKAAVRIPVIANGDIGTPEKAALVLRATSA
                     DGLMIGRAAQGNPWIFREIGHYLATGEKLAPPPVTEVRDTLLDHLETLYAFYGEHSGV
                     RMARKHISWYSKGLRDGAAFRHRVNQVETREAQLAMVREFFDVQDALMPRETGMSGAA
                     DRQILGRAA"
     gene            4656..4898
                     /gene="fis"
                     /locus_tag="AC16FV_09015"
     CDS             4656..4898
                     /gene="fis"
                     /locus_tag="AC16FV_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HIR2850232.1"
                     /translation="MTKDRKAPLAACVKHAMERYFHDLNGEKTAGVYDMVISEVEKPL
                     LEIVMRHVKSNQCKAAEILGINRNTLRKKLKQYKLL"
     gene            5212..6783
                     /gene="purH"
                     /locus_tag="AC16FV_09020"
     CDS             5212..6783
                     /gene="purH"
                     /locus_tag="AC16FV_09020"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR2850233.1"
                     /translation="MTTIRRALISVSDKSGVVEFARALKALKVEILSTGGTAKLLADN
                     GVPVTEVSDYTGFPEMLDGRVKTLHPKIHGGILGRRDLDSHVQTMREHDIPPIDLVVV
                     NLYPFEETVARKDCRLEEAIENIDIGGPTLLRAAAKNHGAVTVIVDAADYGPVLEEMK
                     QRQGSVSDATRFRLATKAFAHTAAYDGAIANYLGREQPGGARADFPDTFNMQFRHAMG
                     LRYGENPHQKAAFYVERRPAEACVATARLLQGKELSFNNIADADAALECVKSFDETAC
                     VIVKHANPCGVAIADSVLDAYKHAYVTDPTSAFGGIIAFNRPLDAATAKAILDRQFVE
                     VIVAPAIRDDAKPILAGKPNVRVLETGAWGATRPAQLDYRRVTGGLLVQDTDQGKITE
                     KELKVVTKRKPNQQELRDLLFAWKVVKFVKSNAIVYCKEGRTIGVGAGQMSRVYSARI
                     ASIKATDAGLEVRGSVMASDAFFPFSDGLDQAAEVGVTAVIQPGGSMRDKEVIEAADQ
                     HGIAMVFTGMRHFRH"
     gene            6787..7209
                     /locus_tag="AC16FV_09025"
     CDS             6787..7209
                     /locus_tag="AC16FV_09025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2850234.1"
                     /translation="MNIAHAETEPDIARCQPVMLELRPHLAGQDFVTLVRRQQKTGYL
                     LAYLEDDGEVRSVAGYRFLEMLCCGKILYVDDLVTHVDCRSAGYGGRLFDWLVEQARA
                     AHCNEFHLDSGVQRFDAHRFYMCHGMSIVWHHFSLKLD"
     gene            7218..>7632
                     /locus_tag="AC16FV_09030"
     CDS             7218..>7632
                     /locus_tag="AC16FV_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR2850235.1"
                     /translation="MRVLVIGGGGREHALAWKIAQSPKAEKVFVAPGNAGTAREARCE
                     NVAINAEDVDGLLRFAQQNKIDLTVVGPEAPLVLGVVDKFRAAGLRIFGPTQNAAQLE
                     GSKAFTKDFLARHKIPTAAYGNFTDVAAAEAYIRKV"
BASE COUNT         1562 a         2500 c         2252 g         1318 t
ORIGIN      
        1 cccctgccgc cgcaccggcg gaacaggcac cggccggaca tgccgtcgcc tcgcccatgg
       61 tcggcacctt ctaccgttcc tccgcccccg gctccgcgcc gttcgtggac atcggcagcc
      121 aggtcaaggt cggcgacacc ctgtgcatca tcgaggccat gaagatgatg aacgagatcg
      181 agtccgacaa ggcgggggtc gtcaaggcca tcctcaagga aaacggccag ccggtggaat
      241 acggcgaggc cctgttcatc atcgaataaa ccatgatcga gaaagtggtt atcgccaacc
      301 gcggcgagat cgcgctgcgc atccagcgcg cctgtcgcgc gctcggcatc cgtaccgtgg
      361 cgctgcattc cgaggccgac cgcgaggcca aatacgtcaa gctcgccgat gaatccgtgt
      421 gcatcggccc ggcgcgcgcc agtgacagct acctgaatat cccggccgtc atcagcgccg
      481 ccgaggtcac cgacgcgcag gcgatccatc ccggctacgg cttcctctcc gagaacgccg
      541 atttcgccga acgcgtggaa caaagcggct tcattttcat cggccccaag gccgagacca
      601 tccgcctcat gggcgacaag atctccgcga tcaaggccat gaaaaaggcc ggcgtaccct
      661 gcgtgcccgg ctccgacgga ccggtcacgg gcagcgacaa ggaaatccac cggatggcgg
      721 cgcaaatcgg ttatccggtg ctgatcaagg ccgccgccgg cggtggcggc aagggcatgc
      781 gcgtagtaca tacagaagcg gcattgctca atgcgttgac gctcaccaag tccgaggcca
      841 aggccgcctt cggcaatgac accgtgtaca tggagaagta cctcgaacac ccgcgccaca
      901 tcgaattcca ggtgctggcc gatacccacg ggcatgcgat tcacctgggc gagcgcgact
      961 gctcaatgca gcgccgccac caaaaggtgg tggaagaaag cccggcgccg ggcctgccgg
     1021 aaaagctgcg acacaaaatg ggtgaacgct gcgccgaggc ctgtcgcaag atcggttacc
     1081 gcggggccgg caccttcgaa tttctttatc aggacggtga gttctatttc attgaaatga
     1141 acacgcgcat acaggtggag catccggtca cggaaatggt cacgggcgtg gacatcgtgc
     1201 gcgaacagct gcgcatcgcc gcgggtgaga aactttccta ccggcaaaag gacatcaact
     1261 ggcgtgggca cgccatcgag tgccgcatca acgccgaaga cccgcggaat ttcattccgt
     1321 cgccggggcg catcaccggc tggcatcccc cgggcgggcc cggcatccgc gtggactcgc
     1381 acgtgtacac caactacttc gtgccgccct actacgactc catgatcgcc aagctgatcg
     1441 cgttcggcga cacgcgcgag atggcgctgg cgcgcatgcg catcgccctc tccgagatga
     1501 tcgtggacgg catcaagacc aacatcccgc tgcaccagga catcatgaac gacgccgcct
     1561 tcatccgcgg cggggtcaac atccattacc tgcagaagaa acttcgactt tgaaaccggg
     1621 gttgcggcgc ggctccctga gttgcctgac accaatccat gccctggctc aaactgaaac
     1681 tgcgcgttac tgaagctcac gcccagccgc tcgcggatgc gttcgaggaa tggggcgcga
     1741 tttcggtgac gctcgaagac gcggccgatc agccgctgtt cgagacgcac tgggacaaga
     1801 atccgatctg gtcgcaggtg aaagtcaccg ggctgttccc ggaacacacc aacactgacg
     1861 acatcctagc gcgggcacgc gagctgatga atctcccgga atcgccgccg catgaaatcg
     1921 atctgctcgg cgacgaggac tgggcgcact catggatggc gcactacaaa ccgcttgagg
     1981 tcgggcacgg cctgtgggtg gtcccaagct ggtgcacgcc gcccgatccc aaggctgtca
     2041 acatcattct cgaccccggt ctcgccttcg gcaccggcga tcatccgacc acctcgttgt
     2101 gtctcgcctg gctgtcggaa caaacgctgg cgggcaaaac cctgctcgat tacggctgcg
     2161 gctcgggcat tctttccatc gccgcgctca agctcggcgc ccatgctgcc tatgccgtgg
     2221 acatcgatgc gcaggcgctc gaggtcacgc gccgcaacgc cgtgcacaac ggcatccgcg
     2281 ccgggttgca tgcgatgccg ccgtcggaac tgccgccgga ttttcaggcc gacatcgtca
     2341 tcgccaatat tctcttcggg acgctgatcg aactcgccgc ggagatcacg gcacgggtgc
     2401 ggccgggcgg ctggctggtg ctgtcgggtc tgctcggcag cgatcaggcc gtggaagtgc
     2461 ggccacatta tgagccgcca ttcgccttcg aacagcgcga acgccaacag tgggtgctgc
     2521 tcgcgggcca caaaccggac taagcttaaa attattatgc ccaaacgaac gcagtaggcg
     2581 accttaaaat atgtacaccc agtgccccgc ctgcctcaca accttcaagg tcacgcctgc
     2641 gcaactggag gcgcgcggcg gcatggtgcg ctgcggtatc tgctctgccg tctttcacgc
     2701 cgagcagcga ctgttacagg tacagcccga gacgaagccg gccaaaaccg cccaggaagc
     2761 ggcggcagcc aaaaatgaca agcgccgatc cgctgtaaaa aaccggcgcc ggtcagggag
     2821 acggcgcagt gacaagctcc agacccttga cgagaaactg acccacgatg ccaatattcc
     2881 cgtcatcacg aaacagccgc tgttcaccag gccacggtta cgcgtcagct cgctcctctg
     2941 ggggttcgga agcctcctgt tgctgacatt gctggccggc caattcgtgt atttctaccg
     3001 cagcgagctg gccaccatcc cggagtggcg gccttatctg gtggatatct gcctttacgc
     3061 cggttgcgaa ctgcgcccgc tgcgcgacgt ggggcgcatc gagctgctcc agaccagcat
     3121 cgcgccacat ccgcaatatg aaaacgcgct gcgcatacgc gccacgctgg tcaaccgcgc
     3181 cgcgtttcgc caggcctacc cgtggatgga agtgagtctc accagcaacg ccggcaatgt
     3241 catcgcgcgc cgcgtcttcg cgcccattca atattcggaa acaccgctgg aggacgcgct
     3301 cacgcccaac gtggtcgcca cggcgctgct ggatgtcacc aatcccgata gcaaggccgt
     3361 cggctacgaa atccgcctgg tcacgccctg atgtggtgcg cctgtcgcac tgaaacggtt
     3421 tttctttttg cttcacccgc ttatcacata atgtgaaacc cgcggatgca gtcattgatt
     3481 ccgtttcatc gtctcgacat ctcgcgtgtt aaaacccacc ctaaaaattc tttttgccgt
     3541 tcatcgcatg ccctcttccc tgtgctggaa gtttctggtt aagattgccg ccctttcccg
     3601 acgttcaggg tagagaaaaa gccgatcatg agaatcggac cttacgagct gaaaaataat
     3661 ctgttcctcg cgcccatggc cggtgtcacc gacaggccct tccgcactct gtgtcggaag
     3721 ctcggtgccg gcatggccgc ctcggaaatg gtgtcatcca attccctgct gtggggcagc
     3781 aaaaagaccc tgcgccgtgc cgatcacagc ggcgaggaag acccgcgcgc ggtgcagatc
     3841 gtgggcagcg accccgccat gatggcgcag gccgcgcgct acaacgtgga caacggcgcc
     3901 cagatcatcg acatcaacat gggctgcccg gccaagaaga tctgcaacgt gtacgccggc
     3961 tcggcgctga tgcagaatga aaaactcatc gtcgaaattc ttgaagcggt cgtgaaaagc
     4021 gtcgaagttc cggtaacact caagatccgc accggctggg accgcgccaa caggaatggc
     4081 ccggccatcg cgcgcattgc cgaggacagc ggcatccagg cactcgccgt gcacggacgc
     4141 acgcgcgccg atgccttcca gggcgaggcc gaatacgaca ccatcgccgc catcaaggcg
     4201 gcggtgagaa tcccggtgat cgccaacggt gatatcggca cccctgaaaa agccgctctc
     4261 gtgctgcgtg ctacctctgc cgacggcctc atgatcggcc gcgccgccca gggcaatccc
     4321 tggatcttcc gcgaaatcgg acattatctg gcgacgggcg agaaactcgc gccaccgccg
     4381 gtcacggagg tgcgcgacac cctgctcgac catctcgaaa ccctgtacgc gttctacggc
     4441 gaacacagcg gcgtgcgcat ggcgcgcaag cacatttcct ggtacagcaa gggcctgcgc
     4501 gacggcgccg ccttccgcca ccgcgtcaac caggtcgaga cgcgcgaagc gcagctcgcc
     4561 atggtgcgcg aattctttga cgtgcaggac gcactaatgc cgcgggagac agggatgtcg
     4621 ggagcggctg atcggcaaat tttagggcgc gcggcatgac caaggatcgc aaggcgccgc
     4681 tcgccgcgtg cgtgaaacat gcgatggaac gctatttcca cgacctcaac ggtgagaaaa
     4741 ccgcaggcgt gtacgacatg gtgatcagcg aggtggaaaa accgctgctc gagatcgtca
     4801 tgcgccacgt caagagcaac cagtgcaagg ccgcggaaat tctcggcatc aatcgcaaca
     4861 ccctgcgcaa gaagctcaag cagtacaagc tgctttaaga attttcgccg cagagacgcg
     4921 gagatcgcag aggtattttc agttaaggaa cccctgatta attcgtcatt ccagccaagg
     4981 gccgaaggcc cgcgagccgg aatccaggaa aatcatatta actggatgcg ggcttccgcc
     5041 ggcatgacga caaccaaatc aatcagacct tccttaattt tcaggaattt tctctgcgtc
     5101 ctctgcgctc tctgcgctct ctgcggcaga taatcatata acttcgattg cggaagcgag
     5161 tcgcggaatt tagaatccgc gttccaaact ttccatttga ttccactctc catgaccacc
     5221 atccgccgtg cgctgatcag cgtttccgac aaaagcggcg tggtcgaatt cgcacgcgcg
     5281 ctcaaagccc tcaaggtcga gatcctgtcc accggaggca cagccaagct gctggccgac
     5341 aacggcgtgc cggtgacaga agtctcggac tacaccggct ttccggaaat gctcgacggg
     5401 cgcgtcaaga cgctgcaccc aaaaatccat ggcggcattc tgggccggcg cgatctcgac
     5461 agccacgtgc aaacgatgcg cgagcacgac atcccgccca tcgatctggt cgtggtcaac
     5521 ctgtatccct tcgaggaaac cgttgcacgc aaggactgcc gcctcgaaga ggcgatcgag
     5581 aacatcgaca tcggcggccc gacgctgctg cgcgcggctg ccaagaatca cggcgcggtg
     5641 accgtcatcg tggacgcggc cgattacggc ccggtactcg aggaaatgaa acaacgccaa
     5701 gggtccgtca gcgatgccac ccgctttcgt ctcgccacca aggccttcgc gcacacggcc
     5761 gcctacgatg gcgccattgc caattacctg gggcgcgaac agccgggtgg cgcgcgcgcc
     5821 gatttcccgg acacttttaa tatgcagttc cggcacgcca tgggcctgcg ttacggcgag
     5881 aacccgcacc agaaggcggc attctacgtc gagcgccgtc cggccgaggc ctgcgtcgcc
     5941 accgcccgcc tgctgcaggg caaagaactg tcgttcaaca acatcgccga tgccgacgcc
     6001 gcactcgagt gcgtgaagtc cttcgacgaa actgcttgtg tcatcgtcaa gcacgccaat
     6061 ccctgcggcg tcgccatcgc cgactcggtg ctcgacgcct acaagcacgc ctatgtcacc
     6121 gatccgacct cggccttcgg cggcatcatc gccttcaacc gcccgctgga cgcggccacg
     6181 gccaaggcca ttctcgaccg ccagttcgtg gaggtcatcg tcgcacctgc catccgtgac
     6241 gacgccaaac cgattctcgc gggcaaaccc aacgtgcgcg tgcttgaaac cggcgcttgg
     6301 ggtgcgacgc gcccggcgca actggactac cggcgcgtca ccggcgggct gctggtgcag
     6361 gacacggacc agggaaaaat cacggaaaag gaactcaagg tcgtcaccaa acgcaaaccg
     6421 aaccagcagg agctgcgcga cctgctgttc gcgtggaagg ttgtcaagtt cgtcaagtcc
     6481 aacgccatcg tgtactgcaa ggaaggtcgt accatcggtg tcggcgccgg ccagatgagc
     6541 cgcgtctaca gcgcgcgcat cgcttcgatc aaggccaccg acgccggcct ggaagtgcgc
     6601 ggcagcgtca tggcatcgga cgccttcttc ccttttagcg acggcctcga ccaggcggcc
     6661 gaggtcggcg tcaccgcggt aatccagccg ggcggctcga tgcgcgacaa ggaggtcatc
     6721 gaggccgccg accagcacgg catcgccatg gtgttcaccg gcatgcgcca cttccgccac
     6781 tgagccatga acatcgcgca cgccgagacc gagcccgaca tcgcgcgctg ccaacccgtc
     6841 atgctggagc tgcggccgca cctggccggc caggacttcg tcaccctcgt gcgccgacaa
     6901 cagaagacgg gttatcttct cgcttatctc gaagacgacg gcgaggtgcg ttcggtggcc
     6961 ggttaccggt tcctggagat gctgtgctgc ggcaagatcc tctacgtcga cgatctcgtg
     7021 acgcacgtcg actgccgctc cgccggttac ggtggccggc tgttcgactg gctcgtcgag
     7081 caggcgcgcg cggcgcactg caacgaattt cacctagatt cgggcgtgca gcgatttgac
     7141 gcgcaccggt tttatatgtg ccatggcatg agcatcgtgt ggcaccactt cagccttaaa
     7201 ttggattagc cgtggcgctg cgcgtgctcg tcatcggtgg cggcggtcgg gaacacgcgc
     7261 tggcgtggaa aatcgcgcag tcgcccaagg ctgaaaaagt cttcgtcgcc cccggcaacg
     7321 ccggcaccgc gcgcgaggcg cgctgcgaga acgtcgccat caatgccgag gacgtggatg
     7381 gcctgctgcg ttttgcgcag caaaacaaaa tcgacctcac cgtcgtcggt cccgaggcgc
     7441 cgctggtgct gggcgtggtc gataaattcc gcgccgccgg cttgcgtatc tttggtccga
     7501 cgcagaatgc cgcgcagctc gaaggctcga aggcctttac caaggatttc ctggcgcgcc
     7561 acaagatccc gaccgccgcc tatggcaact tcaccgacgt ggccgcggcc gaggcttata
     7621 tcaggaaggt tg
//
LOCUS       DBNGZE010000351         2332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1299289, whole genome shotgun sequence.
ACCESSION   DBNGZE010000351 DBNGZE010000000
VERSION     DBNGZE010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1299289"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="metG"
                     /locus_tag="AC16FV_09035"
     CDS             <1..1071
                     /gene="metG"
                     /locus_tag="AC16FV_09035"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HIR2850236.1"
                     /translation="TINGTKMSKSRGTFITARSYLNHLHPQYLRYYFAAKLNGRVEDI
                     DLNFDDFTARINSNLVGKYVNIASRTAGFITERFGGKLGKEAVNKELLHETQNAAEAI
                     AAAYENRDFSEAVRLIMGMADKANAFVDDRKPWLMAKDPTQNGHLQQVCTTSLNLFRL
                     LTIYLSPILPKLAGDVATFLNIPPLTWADAKTLLTGHTINPYQHLATRVDPKNIPALL
                     DEAQKDMKTMAEKTSISIAETSDLARHPILPEISYDDFAKIDLRIARIVKAAHVEGAD
                     KLLELTVDLDGLTRTVFAGIKEAYKPEDLVGRLTVIVANLAPRKMKFGMSEGMVLAAG
                     PGKKDLWILSPDDGAKPGMRVK"
     gene            complement(1084..1620)
                     /locus_tag="AC16FV_09040"
     CDS             complement(1084..1620)
                     /locus_tag="AC16FV_09040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR2850237.1"
                     /translation="MLYAPGNKRYRAPVSRSFKRMSQNNSFVSRGGIWVLAQVPLMLL
                     AFIVPIRFGAGQFAPTEPVQLMGVFVTGLGVLLIAWGFASLGDALTPFPRPLDGAALH
                     RQGAYRLMRHPIYTGVVLVSLGWTLWWLCGIGVLPVLALAIFFDRKAAHEEVWLREKY
                     QDYPDYARRVKKFIPGVY"
     gene            1638..2297
                     /locus_tag="AC16FV_09045"
     CDS             1638..2297
                     /locus_tag="AC16FV_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR2850238.1"
                     /translation="MHETVTIDRIDACLPQTQCTQCGYPRCRAYAEALADGTAGIDQC
                     PPGGDVTIQALATLLHLPPRPLNPRYGAHKPRARAVIDETLCIGCRKCIDACPVDAIV
                     GARKLMHTVIANECTGCELCLPPCPVDCIAMAPAGPVQPGETWPEYTRAETMRWRVRT
                     EKRLERLARTKPVRRRKSADNATSTAATTPLLPNDREKIRADIRAAIDRVKEKRNRGK
                     S"
BASE COUNT          548 a          749 c          641 g          394 t
ORIGIN      
        1 accatcaacg gcaccaagat gtccaagtcg cgcggcacct tcatcaccgc gcgctcgtat
       61 ctcaaccatc tgcacccgca atacctgcgc tattacttcg cggccaagct gaacggcagg
      121 gtcgaggaca tcgatctcaa ttttgacgac ttcacggcgc gcatcaacag caatctggtc
      181 ggcaagtacg tcaacattgc ctcgcgcacc gccggtttca tcaccgaacg cttcggtgga
      241 aaactgggca aggaagcggt caacaaggaa ttactgcatg agacccaaaa tgcggcggag
      301 gcgatagcag cagcttacga aaaccgcgat ttttccgagg cggtgcgcct catcatgggg
      361 atggcggaca aggctaatgc cttcgtggat gaccgcaaac cctggcttat ggccaaggac
      421 ccgacacaga atggacatct gcaacaggtc tgcaccacca gcctcaacct gttccggttg
      481 ttgacgattt atctttcacc tatcctgccg aagcttgcag gagatgtcgc cacattcctt
      541 aatataccgc cgctcacgtg ggcggacgcc aaaacgctgc tcaccggtca caccatcaac
      601 ccttaccagc atctggccac gcgcgtggac ccgaagaaca ttcccgcgct gctcgatgaa
      661 gcccaaaagg acatgaagac catggctgaa aaaacctcca tctcgattgc cgaaaccagc
      721 gacctcgcgc ggcatcccat cctgcccgag atcagttacg acgattttgc caagatagac
      781 ctgcgcatcg cgcgcatcgt caaggcggcg cacgtggagg gcgcggacaa attgctggaa
      841 ctgacggtcg atctcgacgg tctcacccgc actgtattcg ccggtatcaa ggaggcttac
      901 aaaccggagg acctcgtcgg aagactgacg gtcatcgtcg ccaatctggc gccacgcaag
      961 atgaaattcg gcatgtctga aggcatggtg ctggccgccg gtcccggcaa aaaggaccta
     1021 tggattcttt cgccggatga cggcgccaaa cccggcatgc gggtgaagta atcccgcgcc
     1081 gactcagtac acgccgggga tgaacttctt cacccgccgc gcgtagtcgg gatagtcctg
     1141 gtatttctcg cgcagccaga cctcctcgtg cgcggccttg cgatcgaaaa agattgccag
     1201 cgccaacacc ggcagcacac cgatcccgca cagccaccac aaggtccagc ccagactcac
     1261 cagaacgacg ccggtataga ttggatggcg catcaagcgg taggctcctt gccggtgcaa
     1321 tgcggcgcca tccaatggcc gggggaaagg cgtcagggca tcgccgaggc tggcgaaacc
     1381 ccaggcgatc aataacacgc ccaaccccgt cacgaacacg cccatcaatt ggacaggttc
     1441 cgtcggcgca aactgtccgg caccgaagcg gatgggaaca atgaatgcca acagcatcag
     1501 cggcacctgg gccaacaccc atattccacc acgggacacg aatgaattat tctgcgacat
     1561 gcgcttgaaa ctccggctta ccggcgcacg gtaacgcttg ttgcctgggg cgtaaagcac
     1621 ggccatactc ttcggtcatg cacgaaaccg ttaccattga ccggatcgac gcgtgcctgc
     1681 cgcaaacgca gtgcacgcaa tgcggttacc cgcgctgtcg ggcgtatgcc gaagccctcg
     1741 ccgacggaac cgccggcatc gatcagtgcc caccgggcgg cgatgtcact attcaggcac
     1801 tggccacatt gttgcatctc ccgcccagac cgctcaatcc acgatatggc gcgcacaaac
     1861 cgcgtgcccg cgcggtcatc gacgaaaccc tctgtatcgg ttgccgcaag tgcatcgatg
     1921 cctgtccggt ggacgccatc gtgggcgcgc gcaagctgat gcacacggtc atcgccaacg
     1981 aatgcaccgg ttgcgaactg tgtttgccgc cctgtccagt ggattgcatc gccatggcac
     2041 cggccggccc ggtgcaaccc ggagaaacct ggccggagta cacgcgtgca gagaccatgc
     2101 gctggcgggt gcgtaccgaa aaacgcctgg aacgcctggc gcgaaccaaa cctgtgcgcc
     2161 gaagaaaatc agccgacaat gccacgagta cggccgcaac cacgccactc cttcccaatg
     2221 accgggaaaa gatacgcgcc gacatccgcg cggccattga ccgcgtcaag gaaaagcgga
     2281 atcgggggaa atcatgaaat atgaatctct gccgcaaggg tcgcggagat tt
//
LOCUS       DBNGZE010000352         2439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_466304, whole genome shotgun sequence.
ACCESSION   DBNGZE010000352 DBNGZE010000000
VERSION     DBNGZE010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_466304"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="AC16FV_09050"
     CDS             <1..1656
                     /locus_tag="AC16FV_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR2850239.1"
                     /translation="GTLTMNRMEVKQLYAAGRFWEGDAKELAEIPKPFFDVALHCHNL
                     RKSNNTEFLGDPMEVALVRLAQRALPGAVPFPKRDEIPFDSERRRLSTLHDTREGPVL
                     YTKGAPESVLPLCRRIQRGGQPETLTPDLAESLRKAEVTMAEQGLRVLALAYRPGVTE
                     GPLEKLESDLIFVGLVGFRDPPRPEVPRAVRTCHEAGIRVIMVTGDHPHTALSIAREI
                     GLVRTPEAIVINGDRLSRLTNTELQLTLDSPEVVFARVSADQKLRIVQALKRKGETVA
                     ATGDGVNDAPALKQADIGIAMGVSGTDVAREAAHMVLMDDNFASIVAAIGEGRAVYDN
                     MRKFLVYILTHNVAELVPYLAFALFRIPLPLTVIQILAVDLGTDVLPALALGAELPEP
                     EVMQRPPRSPKERLLSWPLLARAYLWLGLLEGAAAMAAFFFVLYAAGWNYGEMLPAND
                     PIYLQATTACLTAIIVMQVVNIFLCRSERGSTFTRGLFLNRLIQIGVAAEIALILLID
                     YTPWGNRLFGTAPISIDVWLYVVPFALAMLLFEELRKALVRRL"
     gene            1775..>2439
                     /locus_tag="AC16FV_09055"
     CDS             1775..>2439
                     /locus_tag="AC16FV_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2850240.1"
                     /translation="MEKINGHSRRAADYERIERAIRYLEANYQRQPDLKELARGAGLS
                     EFHFQRLFRRWAGISPKRFLQYLTAEHAVRVLRQSCTNLDAAFDAGLSGGGRLHDLLV
                     NFHAATPGELKRAGAGLTIQYGFHSSPFGECLIGVTARGVCHLGFVPPEGRRSALAEL
                     TAEWPQARFEEALGATAPIARRLFTRGNGHSPGIDLHVRGTNFQIKVWEALLRIPPGN
                     VVP"
BASE COUNT          471 a          755 c          737 g          476 t
ORIGIN      
        1 ggcacgctca ccatgaaccg catggaagtg aaacagctgt atgcggccgg gcgtttctgg
       61 gaaggcgatg cgaaggaact ggcggaaatt ccaaaaccat tcttcgatgt cgccctccat
      121 tgccacaacc tcaggaaatc caacaatacg gagttcctgg gcgacccgat ggaggtggcg
      181 ctcgtgcggc tcgcgcaacg cgcgctgcct ggcgccgtac cgttccccaa acgcgatgaa
      241 atccccttcg attccgaacg ccggcggctc tcgacgctgc acgacacacg cgaaggcccg
      301 gtgctctaca ccaagggcgc gcccgagtcg gtgctgccac tgtgccggcg gatacagcga
      361 ggcggacaac ccgagacact cacgcccgac ctggcggaat ccttgcggaa agcggaagta
      421 acgatggcgg aacaaggctt gcgtgtcctg gcgctggcgt atcgaccggg agtgaccgaa
      481 ggaccgttgg agaagcttga gagcgatctg atcttcgtcg ggcttgtggg ttttcgcgac
      541 ccaccacgcc ccgaagtgcc ccgggcggtg cgcacctgcc acgaggccgg catccgcgtg
      601 atcatggtga cgggcgatca tccgcacaca gcgctttcga tagcgcgcga aatcggcctc
      661 gtgcgcacgc ccgaggccat tgtcatcaac ggcgaccggc tgagccgact taccaacacc
      721 gagctgcaac tgacgttgga ttctccggaa gtggtgtttg cgcgcgtcag cgccgatcag
      781 aaacttcgta tcgtgcaggc actcaagcgc aagggcgaaa cggtcgcggc gactggcgac
      841 ggcgtcaatg acgcgcccgc gctcaaacag gcggatatcg gcatcgccat gggcgtgagc
      901 ggtaccgacg tggcgcgcga agccgcgcac atggtgctga tggacgacaa cttcgcctcc
      961 attgtggcgg cgatcgggga aggccgcgca gtgtacgata acatgcgcaa gttcctggtc
     1021 tacatcctca cccacaacgt agcggagctg gtaccctacc tcgccttcgc gttgttccgg
     1081 attcccctgc cgcttaccgt cattcaaata cttgcggtcg atctcggtac cgacgtgctt
     1141 ccggcgcttg cccttggcgc cgaattgccg gaaccagaag tcatgcaacg cccgccgcgt
     1201 tctcccaagg aacgtctcct gagttggccc ttgctcgcgc gcgcttacct gtggctcggc
     1261 ctgctggaag gagccgcggc gatggcggcg tttttcttcg tgctgtatgc cgcgggttgg
     1321 aattacggcg aaatgctccc ggccaacgat ccgatttatc tccaagcgac caccgcctgc
     1381 ctcaccgcca tcatcgtgat gcaggtcgta aacatctttc tctgccgcag tgagcgcggc
     1441 tcgacattta cccgagggct ttttctcaat cgcctgatcc agatcggcgt tgcggccgaa
     1501 atcgcgctga ttctattgat cgactacacg ccttggggaa accggctgtt cggcacggcg
     1561 ccgatatcca tagacgtatg gctttatgtt gtgccattcg cgctcgccat gctgctgttc
     1621 gaagaattgc gcaaggcgct ggtgcggcgc ctataaaatc cgtgcagggc tgtctaatct
     1681 gagaaacccc cactgaaaca gatctctgag ccttgaatgg cacagcgcaa gaatcgggtt
     1741 gttatcagcc gcgggtctga cttattctgc gttcatggaa aagattaacg gtcattcacg
     1801 acgcgccgcc gactacgagc gcatcgagcg cgcaattcga tacctggaag caaattacca
     1861 gcgccagccg gacttgaagg aactggcgcg cggcgcgggc ctgagcgaat ttcattttca
     1921 gcggctgttc cggcgctggg ccggcatcag ccccaagcgc tttctgcaat acctcaccgc
     1981 cgaacacgcg gtgcgagtgt tgcgccaatc atgcaccaat cttgacgcgg cctttgatgc
     2041 cgggctgtcc ggcggcggtc gcctgcacga tctgctggtg aattttcacg cagcgacgcc
     2101 gggtgaactc aaacgcgccg gcgccggact gacgatccaa tatggttttc attcaagtcc
     2161 gttcggggaa tgcctgatcg gcgtgaccgc gcgcggcgtt tgccatctcg gcttcgtgcc
     2221 gccggaaggc agacgatctg cccttgcgga actgacagcc gaatggccgc aggcacgttt
     2281 cgaggaagcg ctaggcgcga cagccccgat tgcacgccgg ttattcacgc gcggtaatgg
     2341 tcattcaccc ggcatcgatc tgcacgtgcg cgggacgaac ttccagatca aggtgtggga
     2401 ggcgctgttg cgtattccac cgggaaacgt agtgcctta
//
LOCUS       DBNGZE010000353         4480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_673412, whole genome shotgun sequence.
ACCESSION   DBNGZE010000353 DBNGZE010000000
VERSION     DBNGZE010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_673412"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="AC16FV_09060"
     CDS             <1..1250
                     /locus_tag="AC16FV_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850241.1"
                     /translation="RAAAVAQRVLASKPEPSPALRRTAWTVVAHTDFEKGAFDRAEAN
                     YQQALALTPEKEPSHAKLVERLAAAVYKQGEQSRSAGKLPEAAAHFLRVGKVAPQSPI
                     RANAEYDAAAVFIASKNWPGATQVLENFRRAYPGNPLQADVSSKLAVAYLEQGKWSHA
                     AVEFEALASNKNKDAQIRREALWQAAELHEKAGNQQHAQGAYERYVSQFPGPLEPAIE
                     ARYRLATLSLKQGQSRAQHRWLDELVKADQSGGRERTDRTRTLAANAAMTLAEPYEEA
                     YRQVRLKEPLKKSLKNKKAKMQLALKAYGAAAEYGVAEVATAATYHIAEIYRDFSREL
                     LASQRPKGLSAEELEQYNVMLEEQAYPFEEKAIELHEVNVRRITDRIYDRWVKNSIEA
                     LGKLRPVRYAKVEKSEVIINALR"
     gene            1333..1884
                     /locus_tag="AC16FV_09065"
     CDS             1333..1884
                     /locus_tag="AC16FV_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850242.1"
                     /translation="MVTAPAATEQKAEAVVSPVVQRDFASALSAIKLGRYQDAERVLL
                     ALTREHPELSGPYANLGIAYHRLGRLPEAMEFLKKAIEINPDRAAYYNQLGIVYRQSG
                     QLDKARESYARALEVDPLYLNAHLNIGILYDLYLQEHGKALQHYERYQTLMPAADAQV
                     NKWIVDLKQRQQSSDKTALRSDR"
     gene            1881..2195
                     /locus_tag="AC16FV_09070"
     CDS             1881..2195
                     /locus_tag="AC16FV_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850243.1"
                     /translation="MNHTVRLFVLIICIAGMNARAADRADLEGTSIIGSRELPKVTYI
                     VPWKKPLPGDLVGRPVQSLLDEALAPVDREVFRREVEYHGLIEGGRKIPPPSSDLART
                     RE"
     gene            2229..2876
                     /locus_tag="AC16FV_09075"
     CDS             2229..2876
                     /locus_tag="AC16FV_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR2850244.1"
                     /translation="MNAYSTIVKFFQDSGVFIYPSIMVLTLGLAIAIERFIYISRSRK
                     INRETWEELLPLLKTGKFQQVFQMTAKSDTAIGKIVNYGLSRAKGARRREDIDAAMEE
                     GMMEVVPRLEKRTHYLATFANVVTLLGLLGTIIGLIKAFTAVAQVNPSEKAELLSASI
                     SIAMNNTAFGLMVAIPFLLIHAFLQARTSEIVDSLEAAKVKFLNLVERVKSDMGA"
     gene            2879..3391
                     /locus_tag="AC16FV_09080"
     CDS             2879..3391
                     /locus_tag="AC16FV_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2850245.1"
                     /translation="MSYRKRRLKRHAVELDITAFMNLIVVLVPFLLTSVVFTRLAVLE
                     LNLPAAASGPTELKKDLQLEITIRPQSIEVGDRNVGLIRRIDSTVQGHDYQTLSDLMQ
                     QLKTRYPEKMDATILSEPDTPYDTLVQVMDAVRSARAVQTGSHARVELFPEISVGDAP
                     PAARVGPKEG"
     gene            3397..3894
                     /locus_tag="AC16FV_09085"
     CDS             3397..3894
                     /locus_tag="AC16FV_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2850246.1"
                     /translation="MQDTPLEKRAQRRRRNQTMVDLNLVSLIDIFTILIFFLLANASD
                     VEILPNTKAVTLPESTSEKTPKETVVVVVNDEDIIVQGRKVASVADVMNSGIETIEPL
                     QAELAYQSGRTVIDKNAKDKVNKDITIMGDKEIPYKLLRKVMVTCAHANYSNIAFAVL
                     KKEKG"
     gene            3901..>4480
                     /locus_tag="AC16FV_09090"
     CDS             3901..>4480
                     /locus_tag="AC16FV_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HIR2850247.1"
                     /translation="MSLYVSTALPWTVSAEDEERFKKILKRISIFFILFSLIMPFLPV
                     PKVDREAAEELPPRIAKLVLEQRATPLPPPVKPAEPEAVNARPEPAKAAPKEVKKEVI
                     TEKTRPPVEKTIETARKKASRSGLLAFKDELADLRDNPVTSNIQKNFKSGPGIGPGKG
                     PGVGSTSGPALGAGARAIVTAATTGSGGINTSK"
BASE COUNT          996 a         1316 c         1376 g          792 t
ORIGIN      
        1 cgcgcgccgc cgccgtcgcg cagcgcgtgc tggcgtcgaa gccggaaccg tcgccggcgt
       61 tgcgacgcac ggcgtggacc gtcgtggccc acaccgattt cgaaaagggc gcgtttgacc
      121 gagccgaggc caactatcag caggcattgg cgctgacgcc ggagaaagag ccgagccacg
      181 ccaagctggt cgagcggctg gcggcggcgg tctacaagca gggcgaacaa agccgctcgg
      241 cgggcaaact gccggaggcc gcggcgcact ttctgcgtgt gggcaaggtc gcgccccaat
      301 cgcccatccg cgccaacgcc gagtacgatg ccgcggccgt gtttatcgcg tcgaagaact
      361 ggccgggcgc cacccaggtg ctggagaact ttcgccgcgc ctatccgggg aatccgttgc
      421 aagccgatgt ttccagcaag ctggccgtgg cctatctgga acaggggaaa tggtctcatg
      481 cggcggtgga attcgaagcg ctcgcctcaa acaagaacaa ggacgcccag atccggcgcg
      541 aagcgctgtg gcaggccgcc gaactccacg aaaaagctgg taaccaacag cacgcgcagg
      601 gcgcctacga gcgttatgtc agccagtttc cgggcccgct cgagccggcg atcgaggccc
      661 gctaccgcct ggccactctc agcctgaaac agggacagtc gcgcgcgcaa caccggtggc
      721 tcgatgaact ggtgaaagcc gatcaaagcg gtggacgcga gcgcaccgac cgcacgcgca
      781 ccctggcggc caatgctgcc atgaccctgg ccgaacctta tgaggaggcg tatcgccaag
      841 tccgcctgaa ggaaccgctg aagaagagcc tgaaaaacaa aaaggccaag atgcaattgg
      901 cgctcaaggc ctacggggcg gcggcggaat acggtgtcgc cgaagtggcc accgccgcca
      961 cttaccatat cgccgagatc taccgcgatt tcagccgcga actgctggcg tcccagcggc
     1021 ccaaggggct gtccgcggaa gaactggagc aatacaacgt gatgttggag gaacaggcct
     1081 atcccttcga ggagaaagcg atcgagctgc acgaggtgaa tgtgcgccgt atcaccgacc
     1141 gcatctacga ccgatgggtc aagaacagta tcgaggcgct cggaaagctg cgcccggtac
     1201 gctacgccaa ggtggagaaa agcgaggtga tcatcaatgc gcttcgctaa acagcccatc
     1261 ctcgctggat tagtttttat cgcactgaac atggcgggat gcgcgacttc atcggttcca
     1321 accggcacac gcgtggttac agcgcccgct gccacggagc aaaaagccga agcggtcgtg
     1381 tctcctgttg tgcaacgcga tttcgccagc gccttgagcg ccatcaagct cggtcgctac
     1441 caggatgccg aacgcgtgct gctggcgctc acgcgcgagc atccggaatt gtcgggtccc
     1501 tatgcgaact tggggatcgc ctatcaccgt ctggggagat tgccggaggc gatggagttc
     1561 ctgaaaaagg ccatcgagat caacccggat cgcgcggcat attacaacca gctcggcatc
     1621 gtataccggc aaagcgggca actcgacaag gcgcgtgagt cctatgcgcg cgccctcgag
     1681 gtggacccgc tttatttgaa cgcgcatctc aacatcggaa tcctgtatga cctgtatttg
     1741 caggagcatg gcaaggcact gcaacactac gagcgttatc agacgctgat gccggccgcg
     1801 gatgcgcagg tcaacaaatg gatcgtcgac ctgaagcagc gccagcagtc ttccgacaaa
     1861 accgccttga ggagcgacag atgaatcaca cagtccggct ttttgtgctc atcatttgta
     1921 ttgccggcat gaacgcgcgg gcggctgatc gcgccgatct cgaaggcact tcgatcatcg
     1981 gcagccgtga gctgcccaag gtgacctaca tcgtgccgtg gaaaaagccg ttgccgggag
     2041 acctggtggg gcggccggtt cagagcctgc tggacgaggc cttggcaccg gtagaccgcg
     2101 aggttttccg gcgcgaggtg gaataccacg gcctgattga gggcgggcgg aagattccgc
     2161 cgccatcgag cgacctggcg cgtaccaggg agtaacggtg tgttttgcaa cagcatagga
     2221 gcgagatcat gaatgcatac agtacgatag tgaaattctt tcaggacagc ggtgtcttca
     2281 tttatcccag cattatggtg ctcacgctgg ggttggcgat cgccatcgag cgcttcattt
     2341 acatcagccg ttcgcgcaag atcaatcgtg aaacctggga ggaactcctg ccgctgctca
     2401 agaccggcaa gttccaacag gtgtttcaga tgaccgcgaa atcggacacc gccatcggca
     2461 agatcgttaa ttacggcctg agccgggcca agggcgcgcg tcgccgcgag gatatcgacg
     2521 cggcgatgga ggaaggcatg atggaagtgg ttccgcgtct ggaaaagcgt actcattatc
     2581 ttgctacctt tgccaacgtg gttaccctcc tgggtctgct tggaaccatt atcggtctga
     2641 tcaaggcctt caccgcggtg gcgcaggtca atccctcgga aaaagcggag ttactgtcgg
     2701 cgagtatttc catcgcgatg aacaacaccg cctttggatt gatggtcgcc atcccgtttt
     2761 tgctgattca cgcctttctg caggcacgga cctcggagat cgtggacagt ctggaagcgg
     2821 ccaaggtcaa gttcctgaac ctggtcgagc gcgtgaaatc cgacatgggt gcctgatcat
     2881 gagctatcgt aaacgccgct tgaaaagaca cgcggtggag ctggacatca ccgcgttcat
     2941 gaatctgatc gtggtgctgg tgccgttcct gctgacgagc gtggtgttca cgcgtctggc
     3001 ggtgctggag ctgaaccttc cggccgcggc atcggggccg acggaactca aaaaggatct
     3061 gcagcttgag atcacgatcc ggccgcaatc gatcgaagtg ggtgaccgca acgtcggcct
     3121 gatccggcgt atcgacagca ccgtccaggg acacgattat cagacgcttt ccgacctgat
     3181 gcagcagctc aagacccgtt atcccgagaa gatggatgcc accatcctct ccgagcccga
     3241 cacgccgtat gacacgctgg tgcaggtgat ggacgcggtg cggtcggcgc gcgcggtgca
     3301 aaccggatcg cacgcacgcg ttgagctgtt cccggaaatc tccgtgggtg atgccccacc
     3361 ggccgcgcga gtcgggccga aggagggata aatatcatgc aagacacccc gcttgaaaaa
     3421 cgcgcgcaac ggcgccggcg caaccagacc atggtggatc tcaacctggt gtcgctaatc
     3481 gatattttca cgattctgat tttcttcctg ctggcgaacg cctcggatgt ggaaatcctg
     3541 ccaaacacca aggcagtcac gctgcccgag tccacgtccg aaaagacacc aaaggaaacc
     3601 gtggtggttg tggtgaacga cgaagacatc attgtgcagg gccgcaaggt tgcttcggtg
     3661 gcggatgtca tgaattccgg aatcgaaacc atcgaaccgc ttcaggcgga gctggcatat
     3721 caatccgggc ggaccgtgat cgacaagaac gcgaaggaca aggtcaacaa ggacatcacg
     3781 atcatgggcg acaaggagat tccctacaag ctcctgcgga aagtcatggt tacctgtgcg
     3841 cacgcaaact acagcaatat cgcttttgcc gtgctgaaga aggaaaaggg atagtccgcc
     3901 atgtcacttt acgtctccac cgcattgccg tggacggttt ccgccgagga cgaggaacgt
     3961 ttcaagaaaa tcctcaaacg aatatcgatc ttctttattc ttttcagtct gatcatgccg
     4021 ttcctgcccg tgcccaaggt cgatcgggag gcagccgagg aattgccgcc gcgcatcgcc
     4081 aaacttgtgc tcgagcagcg cgcgactccg ttgccgccgc ccgtgaagcc ggccgagccg
     4141 gaagccgtga atgccaggcc ggaaccggcc aaggccgcgc ccaaggaagt caaaaaggaa
     4201 gtcataacgg aaaagaccag gccgccggtc gaaaaaacca tcgaaaccgc gcgcaagaag
     4261 gcatcgcgct caggcctgct cgcgtttaaa gatgaactgg cggatttgcg cgacaatccc
     4321 gtcacgtcga atatccagaa gaacttcaag tccgggcccg gtattggtcc gggcaagggc
     4381 cccggtgtgg gttcgacgag cggtccggca ctgggcgccg gcgcccgggc catcgtgacc
     4441 gcggccacga ccggtagcgg cgggatcaac acctcgaaac
//
LOCUS       DBNGZE010000354         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_466644, whole genome shotgun sequence.
ACCESSION   DBNGZE010000354 DBNGZE010000000
VERSION     DBNGZE010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_466644"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /gene="mtnC"
                     /locus_tag="AC16FV_09095"
     CDS             <1..458
                     /gene="mtnC"
                     /locus_tag="AC16FV_09095"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043874 - acireductone synthase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HIR2850248.1"
                     /translation="EDKKITPLKSLQGMIWEDGYKKGDFKGHMYEDAVRHLKKWKQAG
                     LRLYVFSSGSVQAQKLLFAHTEYGDLTPLFSGYFDTTIGNKRETDSYRRIAEAIGTPP
                     GDVLFLSDIREELDAARAAGMQVTWLVRDGVPDPNATHRQFRNFEEITL"
     gene            complement(455..1810)
                     /gene="rimO"
                     /locus_tag="AC16FV_09100"
     CDS             complement(455..1810)
                     /gene="rimO"
                     /locus_tag="AC16FV_09100"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR2850249.1"
                     /translation="MSKAISSRPAAQAGLTSIPKVGFVSLGCPKALVDSERILTQLRA
                     EGYAISPDYAGADLVVVNTCGFIDDAVEESLDAIGEALAENGRVIVTGCLGAKGDIVR
                     QTHPQVLAVTGPHALEEVMQSVHAHLPKPHDPYTDLVPPAGIKLTPRHYAYLKIAEGY
                     NHRCTFCIIPSMRGDLVSRDVGEVLQEAENLVKAGVKELLVISQDTSAYGVDVKYRPG
                     FWHGRPVKTRMTDLARALGELGVWVRLHYVYPYPSVDEIIPLMAEGKILPYLDVPFQH
                     ASSRILKLMKRPASAENNLARIEKWREICPDLTLRSTFIVGFPGETEQEFNELLDFLR
                     AAQLDRVGCFAYSAVEGAAANALPDPVPEEVKRERLARFMEVQAKISAEKLQRKVGKT
                     LTVLVDSVEDETVVARSSADAPEIDGVVYIEGARNLKPGDFCQVRVTRASEHDLWAKP
                     A"
     gene            complement(1816..2322)
                     /locus_tag="AC16FV_09105"
     CDS             complement(1816..2322)
                     /locus_tag="AC16FV_09105"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR2850250.1"
                     /translation="MKSIIRLSVFFAGLLLAGTAIAAEGAVGAPVEEPVAKVVWHVDF
                     TDPRRLSAMLQNVNNMVTTYQNQLEDYDIRIVFLAGGIRFVTRDPLKGTPFAEDPEYK
                     KRRPELIQRLQQLQEMHGVKLELCEITREALNLAKEKIIPGVTPVRSGVVRIAELQHQ
                     GFAYLKIE"
BASE COUNT          480 a          752 c          742 g          478 t
ORIGIN      
        1 atgaagataa aaaaatcaca ccgctgaaaa gcctgcaggg aatgatctgg gaggacggat
       61 acaaaaaagg cgacttcaag ggtcatatgt acgaagacgc cgtgcgccat ctgaaaaaat
      121 ggaaacaggc cggcctgcgg ctttatgtat tttcctcggg ctcggtgcag gcccagaaac
      181 ttttattcgc gcacacggaa tacggcgacc tgacgccgct tttttccggc tatttcgaca
      241 ccaccatcgg caacaagcgc gagaccgatt cctaccgcag aatcgccgag gccatcggca
      301 ctcctcccgg agacgtcctg tttctctccg acatcaggga agaactcgac gccgcgcgcg
      361 cggccggaat gcaagtgacg tggctggtgc gggatggcgt acccgatccc aatgcgacgc
      421 accggcagtt ccggaacttc gaagaaatta cgctttaggc cggttttgcc cagagatcgt
      481 gctcgctggc acgggtaacg cggacctggc agaaatcgcc gggcttcaga ttcctggcgc
      541 cttctatata aaccacgccg tcgatctcgg gcgcatcggc gctggaacga gcgaccactg
      601 tttcgtcttc aacactgtcc accagcacgg tcagggtctt gccgaccttg cgctgcaatt
      661 tttccgccga aatctttgcc tgtacttcca tgaagcgtgc caggcgttcc cgtttgacct
      721 cttcgggtac cgggtcgggc aaggcgttgg cggcggcgcc ttccaccgct gaataggcga
      781 agcaaccgac gcggtcgagc tgcgccgcgc gcaggaaatc aagcagctcg ttgaactcct
      841 gctcggtctc gccgggaaaa ccgacgatga aggtcgagcg cagcgtgagg tccgggcaaa
      901 tctcgcgcca tttttcgatg cgcgccagat tgttctcggc gctggccggg cgcttcatga
      961 gtttcaggat gcgcgagctg gcgtgctgaa acggcacgtc gaggtagggc agaattttcc
     1021 cttccgccat gagcgggata atttcgtcca cgctcgggta ggggtagacg taatgcaacc
     1081 gcacccacac acccagttca cccaaagcgc gcgcgagatc ggtcatgcgc gtcttgactg
     1141 gccggccgtg ccagaatccg ggacggtatt tcacgtccac gccgtaggcg ctggtatcct
     1201 gcgaaatcac cagcagttcc ttcacgcccg cctttaccag attctccgcc tcctgcagca
     1261 cctcgccgac gtcgcggctc acgagatcgc cgcgcatgga ggggatgatg caaaacgtgc
     1321 aacggtgatt gtagccctcg gcgatcttga gataggcata gtggcgcggc gtgagcttga
     1381 tgccggccgg cggcacgagg tcggtgtacg gatcgtgcgg tttcggcaga tgcgcatgca
     1441 ccgactgcat gacttcctcg agcgcgtgcg ggccggtcac ggccagcacc tgcgggtggg
     1501 tctgcctgac gatatcgccc ttggcgccga gacagccggt gacgatcaca cgtccattct
     1561 cggccaaggc ctcgccgatg gcatcgagcg actcctcgac ggcgtcatca atgaagccgc
     1621 aggtgttgac taccacgagg tcggcgccgg cataatcggg cgagatcgcg taaccctcgg
     1681 cgcgcagttg cgtcaggatg cgctcggaat ccaccagcgc cttggggcag ccgaggctga
     1741 cgaaacccac cttgggaatg gaagtgaggc cggcctgtgc ggccggccgg gatgaaatcg
     1801 ctttcgacat ggagtctatt ctatcttgag gtaagcgaag ccctgatgct gcagctcggc
     1861 gatgcgcacc acaccggagc gcaccggcgt gacgccgggg atgattttct ccttcgccag
     1921 gttcagggcc tcacgggtga tttcacacag ctcaagcttg acgccgtgca tttcctgcag
     1981 ttgttgcaag cgctgaatca attccggtcg gcgcttcttg tattcggggt cctcggcaaa
     2041 gggcgtgccc ttgagcggat cccgggtgac gaaacggatg ccgcccgcga gaaacacaat
     2101 acgtatgtcg tagtcttcca attgattctg gtaggtcgtc accatgttat tgacgttctg
     2161 gagcatggcg ctgagacggc gcgggtcggt gaaatccacg tgccacacca ccttggccac
     2221 cggttcctcc accggcgcgc cgaccgcgcc ctcggcagca attgccgtac ctgccagcag
     2281 caggccagca aagaataccg acagacgaat gatggatttc atgaccacct cctgtgaatc
     2341 gttcaggcgc ttatgccttg gcgtcataat accgctttga cggcggcgat tcgcgcctcg
     2401 cgcacgcgcg cgccgatatc ggggcccttc ccgccctgcg ccgccagcgc gg
//
LOCUS       DBNGZE010000355         7891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1508788, whole genome shotgun sequence.
ACCESSION   DBNGZE010000355 DBNGZE010000000
VERSION     DBNGZE010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1508788"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1187
                     /gene="lpxB"
                     /locus_tag="AC16FV_09110"
     CDS             12..1187
                     /gene="lpxB"
                     /locus_tag="AC16FV_09110"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR2850251.1"
                     /translation="MTRIGIVAGEASGDLLGAGLIREIRKRLPTASFEGIGGPQMQAV
                     GCRSLFPMDRLSTIGFEGVEKYPEILGIRNKLTEHFLSNPPDLFIGVDVPDFNLGLEE
                     KLKAAGIPTVHYVSPTVWAWRGYRIRKIRRAVDHMLTLFPFEASYYRKHHVPVTFVGH
                     PLADQIDERSSTAAMRRALHLPAKDVVIALLPGSRGSELRRHADLFVKTALWLHKRHS
                     NIHFVAPFVSPETRAIFEEALYRQGAWFLPVTIVANQSRAAMAAADIVLLASGTATLE
                     AALLKKPMVVTYKMSWLSYLLVQPFLHVKLYALPNILAGRKIVPELMQREAVPEKLGN
                     AVEDFLTHRDKTRSLRTVLSGIHRSLRRNADARAAEAVIRLLKPRKDSAKKTSVTVN"
     gene            1191..1790
                     /gene="rnhB"
                     /locus_tag="AC16FV_09115"
     CDS             1191..1790
                     /gene="rnhB"
                     /locus_tag="AC16FV_09115"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR2850252.1"
                     /translation="MSSGFGSRLVAGVDEVGRGPLAGPVVAAAVILDEARPIEGLADS
                     KTLSARRREEFSVLIRERALCWALGRAEVEEIDRLNILQASLLAMQRAVMALALQPEH
                     VLVDGNQVPKLTCSVTAVVRGDVTVPAISAASILAKVARDAEMCELDRCYPGYGFSMH
                     KGYPTRTHIEALQKQGISPIHRRSFGPVRQVLAMSPSCA"
     gene            2510..3346
                     /locus_tag="AC16FV_09120"
     CDS             2510..3346
                     /locus_tag="AC16FV_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850253.1"
                     /translation="MAKSITPSWSWMYSERTQLQVVYQLSNVSYGNSQDVGLYDYDYR
                     SATVTLSNQLSELNQVFVTGGYSKFHVPSTGFDSDTRSFQVGATRKFSSTTQGTLQAG
                     LRNTESFVRGGNTIFSRFTTIDSNGELVDVLIPTGMTQDSRHENTSSVFSGNLERNFE
                     KSLARLAVSRTLSPSGSGGQTEQDAVSFNLRNPLSPRLTLSVNANWQKTSAFEGNITN
                     SDLTYYDFRSGIDWQWLRDLSVGMDYRYTHVNREHENTAADSHAVGVALIYRPLKMSI
                     SR"
     gene            3548..5308
                     /locus_tag="AC16FV_09125"
     CDS             3548..5308
                     /locus_tag="AC16FV_09125"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:NF025179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR2850254.1"
                     /translation="MEMEEQTRDIRDYIVAFRRRQTLVLIIFTVLFITTLLAAFLWPP
                     TYRSTATILIEEQAIPSDLVRSTITTYAWQRIQTISQRVMTRANLLEIVDKHKLYQGK
                     RAFETNEEITERMRNDIKLEPVSTGVIDPRTGNPRVATIAFTLSFDGENPVVTQRVAS
                     ELTTLYLNENIKSRTEKVAETYDFLTSEADKLNQQIAEYETQLATFKEKNANRLPELK
                     DFNMQQMDRIETQLRDTQNELRSLDERKAYIEAELAQVQPSGPVFSSDGQPVLNTEAR
                     LKSMKTEYAVASAKYSPEHPDVIKLKREIEGLEKQTGPVSQRPEQAKELAQLRGELAA
                     SREKYSADHPDVIRLTRQIEALEAGLKEKPRLPETSAAAEKPDNPAYISLKTQLEGIE
                     IGTRAAIAKSDQLRAKLTDYEKRILQTPQAEREYLNILRDYNNAREKYKDIKAKQMEA
                     QVGQELEKERKGERFSLIDPPQLPEEPIKPKRPAILLLGLFFSLASSFGYVVVAESLG
                     NTVRRRSLAAELGMELLSVIPYIETQEDIARKIKTKKLIFTTAAVSLVVVIILAHFLW
                     TPLDVLWFKGLRKADVIIGG"
     gene            5386..6297
                     /locus_tag="AC16FV_09130"
     CDS             5386..6297
                     /locus_tag="AC16FV_09130"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR2850255.1"
                     /translation="MERIKEALERARQERQQSGGALGAAASRGAATATTPSQIAYTQT
                     RHAEVSSEFLRGKRIISGLDPGSFSDAYRILRTQVLQRLRENKWNVLAVTSPGLNEGK
                     TLTAINLAISLAMEVNYTVLLVDADLRHPNVHTYFGLEPEFGLSDYLLSDKPLQELLV
                     HPAGIQGLVVLPGGKPLTNSSEMLSSPKMSRLVEELKTRYPSRIVLFDLPPLLATADA
                     IAFAPYVDAALLVVEEGKTSTQDAQHATELLKDTHLIGAVLNKSLSIPADNRKAGREF
                     LQWNIPWNMVVGRLRMWSSHLFRRKSH"
     gene            6301..7551
                     /locus_tag="AC16FV_09135"
     CDS             6301..7551
                     /locus_tag="AC16FV_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2850256.1"
                     /translation="MYEKFYGLQGKPFSLLPDPEFLYPSKKHRMALTLLEYGLMNQAS
                     FSVITGDIGTGKTTLIRQLLNQMERDMVVGLITNTHPSFGELLQWILMAFNLECGSKD
                     KVEMYKTFMDFLIQHYAANRRTILIVDEAQNMGPQALEELRMLSNVNSEKDQVLQVIL
                     AGQPGLRDNLRDPRLEQFAQRISVDYNLEALSQDETREYIRHRLDIAGGNPDIFESAA
                     CEAVFHYSGGIPRLINLLCDTALVYGYAEQAPRISASLIEDVARDKQQSKIVPLRQPA
                     SAVAEDASSQASELSVGKKGRGTSGPAGRKMRIAIASDSERQRNYLKMMLERSGLKVV
                     AAVPIGEEIIAQLNREHVDVLLMDLDESAHRSRDLDRLIDHVQSQCGVPVLFNDSSSA
                     GTGGAITDLGRKLTLKLTSLIGRG"
     gene            7599..>7891
                     /locus_tag="AC16FV_09140"
     CDS             7599..>7891
                     /locus_tag="AC16FV_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2850257.1"
                     /translation="MAGETILVVGGAGYIGSHMVLDLLQAGHEVVVLDNFSRGHRDLL
                     MGGTFIEGDLGDARLLDGIFSGHRITAVMHFAAFSLVGESVSKPLDYYRNNVA"
BASE COUNT         1890 a         2019 c         2172 g         1810 t
ORIGIN      
        1 cggcaaggtc cgtgacaaga atcggaatcg tcgccggcga ggcttccggc gatttgctgg
       61 gcgccggact gatacgcgaa atcaggaaac ggcttccgac ggcttccttc gagggcattg
      121 gcggcccgca aatgcaggcc gtgggttgtc gcagcctttt tcccatggac cggctttcca
      181 ctatcggttt cgagggcgtc gagaagtatc cggagattct tggcataagg aacaagctta
      241 cggaacattt tttgtccaac ccgccggatc tcttcatcgg tgttgatgtc cctgatttca
      301 atcttggtct cgaggaaaag ctgaaggccg ccggtattcc gacggtgcac tatgtcagcc
      361 ccacggtatg ggcctggcgc ggttaccgca tccggaaaat acggcgcgcc gttgaccata
      421 tgctgaccct gttcccgttc gaggccagct attaccgcaa gcatcatgtg ccggtcactt
      481 tcgtgggcca tccgctggcc gatcaaattg acgagcgatc cagtaccgcc gccatgcgcc
      541 gcgccctcca tctgcccgct aaagatgtcg tgattgcctt gttgcccggg agccgtggca
      601 gtgaactcag gcgccacgcc gacctgtttg taaaaacggc attgtggctg cacaagcgcc
      661 attcgaatat ccattttgtc gccccgtttg tgagtcccga gacacgggcc attttcgagg
      721 aggcgcttta ccggcagggc gcctggtttc tgcccgtgac cattgtggcc aatcagtcac
      781 gcgccgccat ggcggcggcc gatatcgtgc ttctggcctc cggcacggcc acccttgagg
      841 ccgcgctgct caaaaaaccg atggtcgtca cctacaagat gtcctggctg tcctacctgc
      901 tggtccagcc gtttttgcac gtgaaattgt atgccttgcc taatattctg gccggccgca
      961 aaatcgtgcc ggaactgatg cagcgcgagg ccgtgccgga aaaactggga aacgccgtgg
     1021 aggattttct gacccaccgc gacaagaccc gctcattgcg tacggtgctt tccggcattc
     1081 atcgctccct gcgccgaaat gcggatgcgc gcgcggcgga ggcggtgatc aggcttttga
     1141 agccgcgaaa agattcagcc aaaaaaactt cagtcacggt gaattaagcg atgtcgtccg
     1201 gttttggctc aagacttgtg gcgggtgtgg acgaggtagg ccgtggaccg ctggcaggtc
     1261 ctgtcgtcgc ggcggccgtg attcttgatg aagccagacc catcgagggc ttggccgatt
     1321 ccaagacctt gagtgcccgg cggcgcgagg agttttcggt cctgatccgg gaacgcgcac
     1381 tctgctgggc actgggccgg gcagaggtcg aagagatcga tcggctgaat attttgcagg
     1441 ccagcctgtt ggccatgcag cgtgccgtaa tggcgcttgc cctgcagcca gagcatgtat
     1501 tggtcgatgg caatcaggtg cccaaattga catgttccgt gacagctgtc gttcgggggg
     1561 atgtcaccgt gccggcaata tccgccgctt cgattcttgc caaggtcgcg cgtgatgccg
     1621 agatgtgcga actagacagg tgttatccag ggtatggatt ttcaatgcac aagggctatc
     1681 ctacccgcac gcacatcgag gcattgcaga agcagggcat cagccccatt caccggcgca
     1741 gctttgggcc ggttcggcag gtgttagcca tgtcaccttc atgcgcgtaa ctccatgtaa
     1801 acaaggggag ataagcggat gcagcagccc gcaccggcca tgccggcccc gcaaaatcag
     1861 gattttgccc gatgtttcag gtaaacttcg cccagcctgt aaggacaccg gcgatgcagt
     1921 gaacgggttt gttcgaaacg atgcttcgag ttgtgcgcac ggaacaagat tgccagacgg
     1981 atttaagaag ctggaaaata ctcaaaatat tgaaatttcc cctcgctcga catagcaaaa
     2041 gtcacccgta atctctctca ttcagttttt cggtatgccc gtgcttttcg attccggtag
     2101 atatttattt attgtgacca gcatgtgcgc atccctcgcg ataccggtta taggcggagc
     2161 ggccgagtgg actgccgagc ccgccgttag tgctcgacat gaatatagcg acaatgttag
     2221 ctcatccgta catccgcata gcagtgcgcg aggcagcttt attactccca gcctggacct
     2281 cggagtcaat acgccgatat ggcaactaag cgggggagtg tctgccactc agcgtcgcta
     2341 ttcaggacag tcgggtcttg acagtgacga cagggggtcg agactggccg cagcatacag
     2401 aacggagcga aacacctggc aggtgagcgc aagccgcagt ctgaattcgc ttctgtccgg
     2461 cgatttgata agttctgata caggagttct gcagactcag cggcagactt tggcgaagag
     2521 catcacgccc agctggtcct ggatgtattc agaaagaact caactgcagg tagtgtacca
     2581 gttgagcaat gtttcctatg gaaattccca ggatgtcgga ttgtacgatt atgattatcg
     2641 gtcggccaca gtaactcttt ctaaccagct ttccgagctt aaccaggttt ttgtgacagg
     2701 gggatattcc aaattccacg tgcccagcac cggatttgac tctgacactc gaagctttca
     2761 ggtcggcgcc acccgaaaat tttcatcgac cacgcagggc actcttcagg cgggcctacg
     2821 taatacagag tcctttgtgc gcggcggcaa tacgattttt tcgcgtttca caactataga
     2881 tagtaatggc gagctggtag atgtattaat accaaccggc atgacacaag attcccggca
     2941 tgagaacaca agctcggtat ttagcgggaa tcttgagagg aattttgaaa aatcactcgc
     3001 aagattagcg gtgagtcgca cgcttagccc aagcggttcc ggcggacaga cagaacaaga
     3061 cgcagtcagc tttaatctta gaaaccctct gtctccgcgg ttgacgttgt ctgtcaatgc
     3121 caattggcag aaaacgagcg cttttgaggg caatataacc aacagcgatc tcacttatta
     3181 tgatttcagg tctggtattg attggcagtg gttacgagat ttgagcgtgg gcatggatta
     3241 cagatataca cacgtaaatc gtgagcacga aaatacagcc gcagattctc atgccgttgg
     3301 tgtggcgttg atatatcgtc cgttgaaaat gtcaatttca cgataataaa caatgtgtta
     3361 ttaatggtgc attggagggt tgagcgctgc gtggtgaaag cgtctcgcag aaagagataa
     3421 cttatactgt tatggggctc taagagggct gaatccatca cttcagtttt tgtcaggatg
     3481 gccggtttgg gtttcaacgg tatagagcaa atatgaatct gatgcctgaa aaatttttga
     3541 aataattatg gaaatggaag agcagactag agacatacgc gattatattg tggcctttcg
     3601 tcgtcgtcag acactggttc tcattatttt cacggttctt tttataacca ccttgttggc
     3661 ggcttttttg tggcccccaa cctaccgctc caccgccacg atcctgatcg aggaacaggc
     3721 gattccttcc gatctggtgc gctccaccat aacaacgtat gcctggcagc gtattcagac
     3781 gatcagtcag cgagtgatga cgcgcgcaaa tttgctcgag atcgtagata agcacaagct
     3841 ctaccaaggc aaacgtgcct tcgaaacaaa tgaagaaatt accgaaagaa tgcgtaatga
     3901 cattaaactc gaaccagtca gcacaggagt gattgatccc cgaactggca accccagggt
     3961 tgcaacgatc gcgtttaccc tttcttttga tggagaaaat cctgtcgtca cacagagggt
     4021 agcgagcgag ctgacaacgc tgtatctgaa tgaaaacatc aaatctcgaa ccgagaaagt
     4081 cgcggaaaca tacgattttc tcacctccga ggcggataag ctgaatcagc agattgcaga
     4141 atacgaaacg caactggcga ctttcaagga aaagaatgcc aaccggctgc ccgaacttaa
     4201 agacttcaat atgcagcaaa tggatcgcat tgagacgcaa ctgcgagaca ctcagaatga
     4261 attgcgatcc ttggatgaaa gaaaggcata catcgaggca gagcttgccc aagttcagcc
     4321 gagtggacct gtattttctt cagatgggca accggtgctt aatacggaag cgcgcctcaa
     4381 aagcatgaag acagaatatg ccgtggcttc cgcgaaatat tcaccggaac acccggatgt
     4441 gatcaagctc aagagggaaa tcgaagggct cgaaaaacag actggacctg tctcccagcg
     4501 tccggagcag gcgaaggaat tggcacagct tcgtggtgaa ctcgcggcat cgcgcgagaa
     4561 atattctgca gatcatcccg acgtgatcag gctgaccaga caaatcgagg ccctggaggc
     4621 aggtctcaag gaaaaaccgc ggttgcccga gacgagtgcc gcggcagaga agccggataa
     4681 cccggcttat atctcactca aaacgcaact tgaaggaatt gaaataggga cgcgtgctgc
     4741 tatcgccaaa agcgatcagc tcagagccaa gctcaccgat tatgaaaaac gtatccttca
     4801 gacacctcaa gcggaacgtg aatacttgaa tattctgcgg gattacaaca acgcccggga
     4861 aaaatacaag gatatcaaag ccaagcaaat ggaggcgcag gtcgggcagg agctggaaaa
     4921 agagcgcaag ggcgaaagat tctctttgat cgatcctccg caacttcccg aagaaccgat
     4981 taagccaaaa cgcccggcca ttcttctctt ggggctgttt ttttcattgg cttccagctt
     5041 tggttatgtg gtggtggctg agtctctggg caatactgtg cgtcggcgtt ccctggcagc
     5101 ggaattagga atggaattat tgtctgttat tccatacata gaaacgcagg aagacatagc
     5161 cagaaaaata aaaacaaaga agttgatctt tacaaccgct gccgtcagct tggtagtggt
     5221 gattattctg gcccattttc tttggacgcc gctggatgtt ttatggttca aaggcttgcg
     5281 taaagcagat gtaattatcg gtggctagcc aatatggcgg acgcgtgaaa gagcgaataa
     5341 ttttattgct aactatctgt actaaataaa taagacaatc tcaatatgga acgaatcaaa
     5401 gaagcgttgg agcgggcacg tcaggagcgt caacaatcgg gcggtgcctt gggcgctgct
     5461 gcatcccgag gcgctgctac cgctaccacc ccgtcccaaa ttgcttatac ccagacgcgc
     5521 cacgccgagg tctctagcga atttctgcgc gggaaacgca taatttccgg tcttgatccg
     5581 ggttcttttt ccgacgcata cagaatactg cgtacccagg tgcttcagcg tttgcgcgaa
     5641 aacaagtgga acgtactggc ggtaacgagt ccgggactca atgaaggaaa gactctgacg
     5701 gctatcaatc tggctattag tctggcaatg gaggtaaatt acacggttct tcttgtggat
     5761 gcggatctcc gtcatccgaa cgttcatacc tatttcggac tggaacctga gtttgggctc
     5821 agtgattatt tgctgtcgga taaaccactc caagagcttc tggtacatcc cgcaggcatt
     5881 cagggtttgg tggttctccc cggcggcaaa cccttgacca attcctcgga gatgttgagt
     5941 tcccccaaga tgtctcgtct ggtggaggag ctcaaaactc gctacccgtc gcgtattgtg
     6001 ttgtttgatt tgcctccgtt gcttgccacg gcggatgcta ttgcgtttgc cccctatgtg
     6061 gatgccgccc tgctcgtggt ggaggagggc aagacctcca ctcaggatgc ccagcacgcg
     6121 acggaactgc tgaaggacac ccatctgatc ggagcggtac tgaacaaatc gctgagcatt
     6181 ccggctgaca acaggaaagc cggccgggaa ttcctccagt ggaatatccc atggaacatg
     6241 gtagtgggga ggcttcgcat gtggagcagt catcttttcc gtcgcaagag tcactaagcc
     6301 atgtacgaaa agttttacgg tctccagggg aagccatttt cccttttgcc tgatcctgaa
     6361 ttcctgtacc ccagcaaaaa gcaccgcatg gcgctgacgt tgttggaata cggcctgatg
     6421 aaccaggcca gcttcagcgt cattacgggt gatatcggca cgggcaagac cacgctgatt
     6481 cgccagcttc tcaatcagat ggaacgtgac atggtagtgg gattgatcac caatacgcat
     6541 ccctcgtttg gtgaactgct tcaatggata ttgatggcct tcaaccttga atgcggcagc
     6601 aaggacaagg tggagatgta caagaccttc atggatttcc tgatacaaca ctatgccgcc
     6661 aatcgtcgca ccatcctgat tgtcgacgag gcccagaata tggggccgca ggcgctggaa
     6721 gagctgcgta tgctctccaa tgtcaacagc gaaaaggatc aggtcttgca ggtgatactt
     6781 gccggtcaac ccgggttgcg cgacaacctg cgggacccgc gcctggaaca gttcgcgcaa
     6841 cgtatttccg tggactacaa ccttgaagcg ctcagccagg atgaaacgcg tgagtacatt
     6901 cgtcatcgcc tggacatcgc cggcgggaac cccgatattt tcgaaagcgc ggcctgcgag
     6961 gccgtgttcc actacagcgg cggcatcccg cgcctgatca acctgctgtg cgacacggcg
     7021 ttggtatacg gttatgcaga gcaggcacct cgtattagcg cgtctttgat tgaagatgtc
     7081 gcgcgcgaca agcagcaaag caagattgtc cccttgcggc aaccggcgag tgcagttgct
     7141 gaagatgcaa gcagccaggc gtcggagttg tcagtaggga aaaaaggccg tggcacttcc
     7201 ggaccggccg ggcgaaaaat gcgtattgcc attgcttcag attcggaacg acaacgcaat
     7261 tatctcaaaa tgatgcttga gcggtccgga ctcaaggtag tggccgcggt tcctatcggc
     7321 gaggaaatca tcgcgcaatt gaacagggag catgtggatg tattgttgat ggatctggat
     7381 gaaagcgccc accgctcgcg tgatctggac cgtctgattg atcatgtaca gagtcaatgc
     7441 ggggtgccgg ttctgttcaa tgacagttct tcagctggca ctggcggagc catcactgat
     7501 ctcggtcgca agctgaccct caagctcaca tcccttatcg gtcgaggctg acgtcttctg
     7561 cggctgtgca atctcatcca ggcacataat gcaacctcat ggccggtgaa accattcttg
     7621 tcgtaggcgg tgcgggctat atcggttcgc acatggtgct cgatctgctt caggccgggc
     7681 atgaagtggt tgtgctggac aatttttcgc gtgggcaccg cgatctattg atgggcggga
     7741 ccttcattga gggagacctg ggcgatgcga ggctgctgga cggcattttc agcgggcaca
     7801 gaatcaccgc cgtcatgcat ttcgccgctt tctcgctggt gggcgagtcc gtatccaaac
     7861 cgctcgatta ttacagaaat aacgtggcaa a
//
LOCUS       DBNGZE010000356         4257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1717344, whole genome shotgun sequence.
ACCESSION   DBNGZE010000356 DBNGZE010000000
VERSION     DBNGZE010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1717344"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="AC16FV_09145"
     CDS             <1..621
                     /locus_tag="AC16FV_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR2850258.1"
                     /translation="EFAANHNKNDKVSGKIKSITDFGIFVGLEGGIDGLIHLSDLSWA
                     QPGEEAVRNYKKGDEIEAVILAVDPERERISLGVKQLDGDPFASYVTAHGKGSIVKGT
                     VTSVETKGAVVKLADEVEGYLRASDIARERVEDARSVLKDGDEVEAKITTIDRKNRKI
                     SLSVRAKDMEEENEAVQEYARKAGSATSSLGDKLKEKLGGHDSSDA"
     gene            790..1077
                     /locus_tag="AC16FV_09150"
     CDS             790..1077
                     /locus_tag="AC16FV_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR2850259.1"
                     /translation="MTKSELISLLAAKQPQLDYRDVELAVKELLEQMSAALSSGDRIE
                     VRGFGSFSLHYRPPRTGRNPKTGAAVQVPDKFVPHFKPGKELRERVNNGTE"
     gene            1210..1506
                     /locus_tag="AC16FV_09155"
     CDS             1210..1506
                     /locus_tag="AC16FV_09155"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR2850260.1"
                     /translation="MKRIIYTILAILVLLVGIAFAIQNKQAIEINYYFGLKWSGPLSL
                     ALLTSFAIGVLAGYLASLRMVVRMQRQLVQARKEIRHIEQEVINLRALPIKDVI"
     gene            1496..2668
                     /gene="lapB"
                     /locus_tag="AC16FV_09160"
     CDS             1496..2668
                     /gene="lapB"
                     /locus_tag="AC16FV_09160"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR2850261.1"
                     /translation="MSFNFDATDLLWLLLPLAAASGWLVARFEQKRRVQKSLDLPSAY
                     FKGLNFLLNEQPDKAIEVFIRVLEVNSDTVETHLALGNLFRRRGEVERAIRIHQNLIA
                     RPTLDREQRSQALLELGQDYLKAGLFDRAENLFLELAEIRAHSEQALRLLLHIYQQEK
                     EWEKAISVVRKLARASGKDMNSMIAHFYCELAEQDYAHHNHSSARERLKHALAADDKC
                     VRASILLGDIDVQEGKYREAIEQWQRIEKQDIHYLGEVAERIAEGFRKLGDEQGLYDY
                     FHVSLRRHGGVSLALTFADIINRREGVEAAEKFIIDWLRRAPNVHGLHKLLELNLVKA
                     KDSARNDLLMLKGIIEELRTQHLGYACAECGFKGKSLHWLCPGCNHWNTIKPVREE"
     gene            2668..3366
                     /gene="pyrF"
                     /locus_tag="AC16FV_09165"
     CDS             2668..3366
                     /gene="pyrF"
                     /locus_tag="AC16FV_09165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR2850262.1"
                     /translation="MVGPRVIVALDFADAGTAQTFVQRVTPAQCRLKVGMELFTAAGP
                     ELVRRLVSRGFDVFLDLKYHDIPNTVAHACTQAAELGVWMLNVHALGGMRMLLAAREA
                     IENSKHSPLLVGVTLLTSHGDEELVQIGLERGTERQVERLAGLVRDAGLDGVVCSPVE
                     ASLLRRRFGKEFVLVTPGVRQAGDAADDQRRTRTPAEAVAEGADYLVIGRPVTRAQNP
                     AAVLDAINREIRDR"
     gene            3480..>4257
                     /locus_tag="AC16FV_09170"
     CDS             3480..>4257
                     /locus_tag="AC16FV_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HIR2850263.1"
                     /translation="MLPQLLEKITRARVLVVGDAMLDRYWYGDVERISPEAPVPVVAV
                     KEAKELPGGAANVARSVRALGARCALFSISGDDREADSLEQLLSKDDIRCRLERDKLL
                     NTTVKLRVVSKHLHQQMLRIDFDCPVSKDARVKLLDAYLKHLSEYDAVIISDYGKGGL
                     GYIQEMIQAARAASRPVVIDPKGDDYSGYRGASLITPNRKEFEQVAGRFHDNAELERK
                     AARMAEELELGGVLVTRGEDGMSLIERDGRVLHIKARAREV"
BASE COUNT          891 a         1192 c         1349 g          825 t
ORIGIN      
        1 gaattcgccg ccaaccacaa caagaacgac aaggtcagcg gcaagatcaa gtccattacc
       61 gacttcggca tatttgtcgg cctggaaggc ggtatcgacg gtcttatcca tctgtccgac
      121 ttgtcctggg cacaaccggg cgaagaggcc gtgcgcaatt acaagaaggg cgatgaaatc
      181 gaggccgtta tcctggccgt ggaccccgag cgtgaacgca tttcgctcgg cgtgaagcag
      241 ctggacggcg atccgttcgc gagctatgtg accgctcacg gcaagggctc catcgtgaag
      301 ggcaccgtga cgagcgtgga aaccaagggc gcggtcgtca aactggcgga cgaggttgag
      361 ggttacctgc gcgcctccga tatcgcgcgt gaacgcgtcg aagacgcccg ttccgtgctg
      421 aaggatggcg acgaggtcga ggccaagatc accaccattg atcgcaagaa ccgcaagatc
      481 tcgctttccg tccgggccaa ggacatggaa gaagagaacg aggccgtgca ggagtatgca
      541 cgcaaggccg gttcagccac ctcttcgctg ggcgacaagc tcaaggagaa gctgggtggg
      601 cacgacagct cggacgccta gaacgggttt tgcgggaatc agggtaaata cgccggaatt
      661 cgcgtaccgg cacggataaa tggggctatc cggcgtttgt gacgaggacc atggcggggt
      721 gtattttgtc ccatgcccgc caaaagtcct tgaaatcttt ctacttattt tctataggta
      781 gagtgagata tgacgaaatc agaactcatc agcttgctcg cggccaagca gccacaactg
      841 gattaccggg atgtggagct ggcggtcaag gaactcctgg aacagatgtc tgctgccctg
      901 tcctcgggcg accgcatcga ggttcggggt tttggcagct tttccctgca ttaccgtcca
      961 ccgcggaccg gccgcaatcc caagaccggc gcggcggtgc aggtgccgga caagtttgtt
     1021 ccccacttca aacctggcaa ggaattgagg gagcgcgtca ataacggcac ggagtgacgc
     1081 gactttcccg gacgtggaat gcaaaacggc atgtgagtca cccatgtcgt ttttttgtga
     1141 ccaaaaataa gccaaactga ccacctatac gacggcggcg ctgcggcacg gccgattcaa
     1201 ggaggcgtta tgaagcggat catttatacg attctggcga ttctcgttct gctcgtcggt
     1261 atcgcgtttg ccatccagaa caagcaggcg atcgagatca actactattt cggtctcaaa
     1321 tggagcggac cgctgtcgct ggcgctgctg accagctttg ccatcggcgt gctggccggc
     1381 tatctcgcca gcctgcgcat ggtggtgcgg atgcagcgcc agctggtgca ggcccgcaag
     1441 gaaatccgtc atatagagca ggaagtcatt aatctccgag cgctgcccat taaagatgtc
     1501 atttaatttt gatgccaccg acctgctgtg gctgttattg ccgctggcgg ctgcttccgg
     1561 ctggctcgtg gcgcggttcg agcagaagcg gcgcgtccag aagtctctcg atctcccctc
     1621 cgcctatttc aagggcctca atttcctgct gaatgagcag cctgacaagg caatcgaagt
     1681 ttttatacgc gtgctggaag tcaattcgga taccgtggag acacatctgg cgctgggaaa
     1741 cctgtttcgc cgccgcggcg aagtcgagcg cgccatccgt attcaccaga acctgatcgc
     1801 gcggcccacg ctcgaccggg agcaacgcag tcaggcgctg ctggagctgg gacaggatta
     1861 tctaaaggcc ggcctgttcg accgcgccga gaacctgttc ctggagctgg ccgagattcg
     1921 cgcccacagc gagcaggcgc tgcggttgct gctgcatatc taccagcagg aaaaggagtg
     1981 ggagaaggcg atttccgtgg tgcgcaaact ggcgcgtgcg tccggcaagg acatgaacag
     2041 catgatcgcg cacttctatt gtgaactggc ggagcaggat tacgctcacc acaatcattc
     2101 ctccgcacgc gagcgcctga aacatgcgct ggcggcggac gacaagtgcg tgcgtgccag
     2161 tattctgctc ggggatatcg acgtgcagga aggaaaatac cgcgaggcca tcgagcaatg
     2221 gcagcggatc gagaaacagg acattcatta cctcggcgag gtggccgaac gcatcgccga
     2281 gggtttccgg aagctgggcg atgagcaggg actttatgat tattttcacg tttctctgcg
     2341 gcgtcacggc ggtgtgtcgc tggcgctgac ctttgccgat atcatcaatc gtcgcgaggg
     2401 cgtggaggcg gcggaaaaat tcatcattga ctggctgcgc cgcgcgccga atgtccatgg
     2461 attgcacaaa ttgctggagc tcaacctggt gaaggccaag gattccgccc gcaacgacct
     2521 gctgatgctg aagggcatca tcgaggaact gcgcacgcag catctgggtt atgcctgcgc
     2581 ggagtgcggc ttcaagggca agtcactgca ctggttgtgc cccggctgca atcactggaa
     2641 caccatcaag ccggtgaggg aggaatgatg gtgggaccgc gcgtcattgt cgctctggat
     2701 tttgccgatg ccggcacggc acaaacattc gtgcaacgtg tcacgcctgc gcaatgccgg
     2761 ctcaaggtcg gcatggagct gttcaccgcg gccggccccg aattggtgag gcgcctggtc
     2821 tcgcgcggtt ttgatgtgtt ccttgatctc aaatatcacg atattcccaa tacggtggcc
     2881 catgcctgta cgcaggcggc tgaactgggt gtatggatgc tgaatgtgca tgcccttggc
     2941 gggatgagga tgctgctggc ggcgcgcgaa gccattgaga attcgaaaca tagtccgtta
     3001 ctcgtcggcg tgacgcttct gacgagtcac ggcgatgaag aacttgtgca gatcggactg
     3061 gagcgtggca cggaacggca ggtggagcgt ttggctggtc tcgtgcgcga cgccggtttg
     3121 gacggtgtcg tctgctcgcc cgtcgaggcc tcgttgttgc ggcgacgttt tggaaaggag
     3181 tttgttctcg tcacccccgg cgtgagacag gccggggatg ccgcggacga tcagcgccgc
     3241 acgcgcacgc ccgccgaagc cgtggccgag ggcgcggatt atctggtgat cggccggccg
     3301 gtgacgcgcg cacaaaaccc cgcagcggtg ctggatgcca tcaatcgtga aatccgggat
     3361 cgttgaaatt ccacgggacc tcgccaatcg acagccatga gaaatgcccg gataataccg
     3421 tgcccagcga ggtccgaatg cgccgggcga tacagccatt gccaggataa ccaataaata
     3481 tgttgccgca gctccttgaa aaaattaccc gggccagggt gctggtggtg ggagacgcca
     3541 tgctggaccg gtactggtac ggtgacgtcg agcgcatttc cccggaagca ccagtgcccg
     3601 tggtggcggt gaaggaagcg aaagagctcc cgggcggcgc cgccaacgtg gcgcgcagcg
     3661 tacgggcgtt gggcgcccgg tgcgccttgt tttccatcag cggcgacgat cgcgaggcgg
     3721 acagtctcga acaactcctg agtaaagatg acatccgctg tcgtcttgaa cgggacaagt
     3781 tgctgaacac caccgtcaag ctgcgtgttg tctccaaaca tctgcaccag caaatgttgc
     3841 gcatcgattt cgattgccca gtcagcaagg acgcgcgcgt gaagctgctc gatgcctatc
     3901 tcaaacatct ttccgaatac gatgccgtga tcatttcgga ttacggcaag ggcgggctgg
     3961 gctacattca ggaaatgatc caagctgcgc gtgccgcctc ccgtccggtg gtgatcgacc
     4021 ccaagggcga cgactacagc ggctaccgcg gcgccagcct gatcacgccg aaccgcaagg
     4081 agttcgagca ggtggccggc cgtttccacg acaacgccga gctcgagcgc aaggccgcgc
     4141 gcatggccga ggagctcgag ctgggcggcg tgctggtcac gcgcggcgag gacggcatga
     4201 gcctcatcga acgcgacggt cgcgtactgc acatcaaggc gcgggcacgc gaagtgt
//
LOCUS       DBNGZE010000357         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_953489, whole genome shotgun sequence.
ACCESSION   DBNGZE010000357 DBNGZE010000000
VERSION     DBNGZE010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_953489"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..535
                     /locus_tag="AC16FV_09175"
                     /pseudo
     CDS             8..535
                     /locus_tag="AC16FV_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829284.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            787..1272
                     /locus_tag="AC16FV_09180"
     CDS             787..1272
                     /locus_tag="AC16FV_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850264.1"
                     /translation="MRSRWLQIRGDPSVRQFVFEQARVANEFDKHIDEVLARVEVLLL
                     GHGVFHAKVHFSTGQVTLWLLNDPLRYRVHVKEEFLDPDLCNIYRRQPYTNEALVPSP
                     EISRVLAEFKRLRTLDNHIYLRAGSLNVVNGLVGLNFSCDGSHYLNYAEFLARAGELY
                     V"
     gene            1502..1945
                     /locus_tag="AC16FV_09185"
     CDS             1502..1945
                     /locus_tag="AC16FV_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2850265.1"
                     /translation="MSSKPFILKPGDRKPALNVIGCQVTVLASGADIRDQQITVQSGD
                     EGMGPPPHSHDWDESFYVIKGQVQFMCGGETVMCTAGTLVHVPGGTVHAFSFGAGGGE
                     MLEITGRQSNAVPMFTALDREIPPGPPNVAKVIQVLGENGVKVHI"
     gene            2193..>2797
                     /locus_tag="AC16FV_09190"
     CDS             2193..>2797
                     /locus_tag="AC16FV_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850266.1"
                     /translation="MIHGFTRVRLGFFLFFLFLISGCSKEFIYPEDERSQAEPVRSAD
                     WIAMLPEGETKRQVILGCTPCHQIGPPVAYRKSLEEWREVIARMKKTDDSLELNLIRL
                     DTEELAQWLTANAKMPKHGRYFEAAKADIREYPAGAKTGFYHDMAVSGGRAWTADYFG
                     NKLYGTDIESGAVESFDIPVTVEPGKPGGAHQIDTTRDGMV"
BASE COUNT          566 a          820 c          852 g          559 t
ORIGIN      
        1 ccactagttg ctgccgtgga tgacctgtgt cggcaatgtc tatctcgccg tcgagcgcgt
       61 gttcggcgat accgaaagcc gcgccgacct gaagcggcgc acgcgcgaag cgctggcgct
      121 ggtcggcctg tcgcacgcgg agcagaaata cccgcatgag atttccggcg gcatgaaaca
      181 gcgcgtcggc atcgcgcgcg ctctgtcgat gcagccgaaa gtattgctgc tcgacgagcc
      241 actgggggcg ctggatgcgc tcacgcgcgc caacctccag gacgagctga tgcgcatcgt
      301 cgcgaccgcg ggcagtacga cgctcatggt gacgcacgac gtggatgagg cggtgctgct
      361 gtcggaccgc atcgtcatga tgaccaacgg gccggcggcg accatcggtg agattctcga
      421 aatcgacctg ccgcgtccgc gcgcgcggct ggagctggtg caggacgcgc attacctgcg
      481 cctgcgcggc gaggtgctca agttcctgta cgcgcgtgag gtcaaggcgg cctgaatcat
      541 gagatcacta cctaatccgt cattccggcc aagggccgaa ggcccgcgag ctggaatcca
      601 ggaaattaat atgcaactgg atgccggctt tcgccggcat gacgaaaact gagttaaagg
      661 gaaacatgtt cacgcaaacc atgatacaga atctgggcgc gctcaaggtc gggtggtatt
      721 ggttggtagt agtgttggcg cgcggtatcc atgaggtcag gaagctgaaa caggagaagt
      781 aagaccatgc gctcacggtg gctgcaaatc cgtggcgatc cctcggtgcg tcagttcgtg
      841 ttcgaacagg cgcgcgtggc gaatgaattc gacaagcaca tcgacgaggt gctggcgcgc
      901 gtcgaggtgc tgctgctggg gcatggcgtg tttcacgcca aggtgcattt ctccaccggc
      961 caggtaacgc tgtggctgct caacgacccg ctgcgctacc gcgtgcacgt caaggaggag
     1021 ttcctcgacc ccgatctgtg taacatctac cggcgccagc cctacaccaa cgaggccctc
     1081 gtgccatccc cggagatttc ccgggtgctg gctgagttca agcgcctgcg cacgctggac
     1141 aaccacatct acctgcgcgc cggctcgctc aacgtggtca acggcctcgt cggcctcaat
     1201 ttctcctgcg acggcagcca ttatctcaac tacgctgaat ttctcgcccg ggcgggcgag
     1261 ttgtacgtct gatattgctg tcattcccgg acatatcggg cggtatgggc gttgtctatt
     1321 agtgattact gatatacctc agtaattttt cttgtacttc ataataatat tcacatccaa
     1381 taataccgac acctgcggca gaatattgcc gcagtatccg tatactcaat gttgggcgac
     1441 aagagtcatt aaccacgtcc gcgcgaatac accggactga ttctcacagg agggctatct
     1501 catgagctcc aagccattca tcctcaaacc gggtgatcgt aagcccgctc ttaacgtcat
     1561 cggctgtcag gtcaccgtgc ttgcatcagg ggcagatata cgagaccagc agatcacggt
     1621 gcagtcgggt gacgaaggaa tgggaccacc gccacatagc cacgattggg acgagtcgtt
     1681 ttacgtgatc aaaggccagg ttcaattcat gtgtggtggt gagactgtga tgtgcacagc
     1741 gggcaccctg gtacatgttc cgggcggtac ggttcatgcg ttcagctttg gtgctggcgg
     1801 cggtgaaatg ttggaaatca ctggaaggca aagcaatgcg gttccgatgt tcactgcact
     1861 ggatcgcgag attccgcccg gtccgcccaa cgtcgcaaaa gtgatccaag ttctaggcga
     1921 gaacggcgtc aaggttcaca tttgatgcgc tgccagtact tgttgcccaa caacgcgctc
     1981 cactcggacg ccccccgcgt cgcttcgcaa gctcgctccg cgggggttcc ctcgctctct
     2041 acgctcgctc gacgcctgtg agcgcgggcg ttagccgccc tcaccagaat tacgatctgt
     2101 ccctgtttat agattcactc ttaccgctgt gaaataattc ctccacccgc ggcccagcgc
     2161 gggtgctttc cactacaagc gaggagatgg cgatgatcca cggattcacg cgggtgcggc
     2221 tgggattttt cctgttcttc ctgtttctga tctccggttg ttccaaggaa ttcatttatc
     2281 ccgaagatga gcggtcgcag gccgagccgg tgcgttcggc cgactggatt gccatgctgc
     2341 ccgagggcga gaccaagcgg caggtgatac tggggtgcac gccatgccac cagatcggcc
     2401 cgccggtggc ataccgcaag tcgctcgagg aatggcgcga ggtgatcgcg cgcatgaaaa
     2461 agaccgacga cagcctcgaa ctcaacctca tccggctgga taccgaggaa ctggcgcagt
     2521 ggctgacggc caacgccaag atgcccaagc atgggcgcta cttcgaggcg gccaaggcgg
     2581 acatccgtga atacccggcc ggcgccaaga ccggtttcta tcacgacatg gcggtgagcg
     2641 gcggccgggc ctggaccgcg gattacttcg gcaacaagct ctacggcacc gatatcgaga
     2701 gcggcgcggt ggagagtttc gatattcccg tgacggtcga gccgggcaag ccgggcgggg
     2761 cgcaccagat cgacaccacg cgcgacggca tggtctg
//
LOCUS       DBNGZE010000358         4379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1300401, whole genome shotgun sequence.
ACCESSION   DBNGZE010000358 DBNGZE010000000
VERSION     DBNGZE010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1300401"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="AC16FV_09195"
     CDS             <1..1559
                     /locus_tag="AC16FV_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR2850267.1"
                     /translation="LQRRLDVVDFATPVQTIDVFAQGKNTRMIVTPKGKYEHLAYQTG
                     NVFTVSVKPVIEKPDEKKVDEFGYSGEKLSLNFQNIEARAALQVLADFTGLNFVVSDT
                     VKGSLTLRLKDVPWDQALDLILDSKNLAMRRKGNVITVAPAPEVAAKEKANLEATKAV
                     VELEPLVSELIQINYAKADDIAALLKSIKQVTTASAQEHPVFGSSATGASIEKSSVAN
                     TLLSPRGQVTVDQRTNSLLIQDVASKIREVRKLIAQLDQPVRQVMIESRLVEATDNFS
                     KSLGVRFGTKYTDTSAGSGRSGAVSGSVSDSSSIVSAGTLTANSSGLNVNLPSAGIGS
                     SPAGAIGITLAKIGSNGGLLNLELSALEQEGKGKVISSPRVITANQKPARIEQGQERV
                     FTTNVLGVGSVVTKKATLKLEVTPQITPDDRVNMEVNITKDNFADAVTGVLNVKEIKT
                     QVLLDNGETVVIGGIYEQDKNDTVTKVPLFGDLPLLGWLFKSKEAKDNKTELLIFLTP
                     RILSDNLSLR"
     gene            1637..2179
                     /gene="aroK"
                     /locus_tag="AC16FV_09200"
     CDS             1637..2179
                     /gene="aroK"
                     /locus_tag="AC16FV_09200"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HIR2850268.1"
                     /translation="MSRLDNIFLIGPMGAGKSTIGRHLAELLNKDFRDSDQEIEKRTG
                     AGIPLIFEIEGETGFRSRESSVLDDLTRQSNLVLATGGGAILSPDNRKILHERGVVVY
                     LHAPLETLLSRTRRDRHRPLLQTADRRRTLEDILKAREPIYRQAADVVIETSHRSPVS
                     VAREIVRKLESHSKNENLAS"
     gene            2160..3245
                     /gene="aroB"
                     /locus_tag="AC16FV_09205"
     CDS             2160..3245
                     /gene="aroB"
                     /locus_tag="AC16FV_09205"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR2850269.1"
                     /translation="MKTLPLDLGERSYPIYIGSGLLGQTELLKKHVVGSRVAIVTNET
                     VAPLYLSKVRSHLSMLKPVEVILPDGEQYKTLEILNRIFDALLGARCDRRTTLIALGG
                     GVIGDMAGFAAACYQRGVPFIQVPTTLLSQVDSSVGGKTGVNHPLGKNMIGAFYQPRA
                     VIIDTDTLNTLPDRELSSGLAEVIKYGLIRDPEFFAWLETNLDKLLARDPEALAFAIH
                     RSCRNKAEVVAADERESGVRATLNLGHTFGHAIETGMGYGEWLHGEAIAAGMTMAADL
                     SRRLGWLTTAETTRIEKLMKRARLPVRAPGSLSSARFLELMAVDKKVLDGRLRLVLLK
                     PLGEAVITDEYPRTGLEATLASMREAA"
     gene            3245..>4379
                     /locus_tag="AC16FV_09210"
     CDS             3245..>4379
                     /locus_tag="AC16FV_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR2850270.1"
                     /translation="MLASYAAADKTSRGRRYPETTPTNRSEFQRDRDRIIHSAAFRRL
                     EYKTQVFINHEGDLFRTRLTHSIEVAQIARSIARALGLNEDLTEAIALAHDLGHTPFG
                     HSGQDALNECLRDYGGFEHNLQSLRVVDVLEERYAEFPGLNLTFETREGILKHCSLKN
                     ARELGELGKRFLEKTHPSLEAQLVNIADEIAYNNHDVDDGLRSGLLTLDQLKPITIFA
                     GQYDEVLRRYPDIPPRRAIYETIRRMINQVVTDLIENSRRRIAQSGIKDIEDVRHQPE
                     PLIAYTTEVAEKSRELKRFLRQNLYAHERVTRMMGEAVKIVRELFEAFMKDATLLPPQ
                     YQDKLRQPNGAGEQARIVADYIAGMTDRYAMREHTRLFGRSGAP"
BASE COUNT          930 a         1336 c         1303 g          810 t
ORIGIN      
        1 agctgcagcg ccgtctcgat gtggtggact tcgccacgcc ggtgcagacc atcgacgtgt
       61 ttgcccaagg caagaacaca cgcatgatcg tgacacccaa gggcaaatat gagcacctgg
      121 cgtaccagac cggcaatgtc tttaccgtca gtgtcaaacc ggtaatcgaa aaaccggacg
      181 agaagaaggt ggacgagttc ggctattccg gggaaaagct ctcgctcaat ttccagaata
      241 tcgaggcgcg cgctgccctg caggtgttgg cggatttcac cggtctcaat tttgtcgtga
      301 gcgatacggt caaaggatct ctcacgctgc gtttgaaaga cgtgccgtgg gaccaggcgc
      361 tcgacctgat tctggatagc aagaatctgg cgatgcgtcg caagggcaac gtgatcacgg
      421 tggcgcccgc gcccgaggta gcggccaagg aaaaggccaa tctggaggcg accaaggcgg
      481 tggtcgagct tgaaccgctg gtgtcggaac tcattcagat caactacgcc aaggctgacg
      541 acattgcggc tctgctcaag tcgatcaagc aggtgacgac ggcgagcgcg caggagcatc
      601 cggttttcgg tagctccgcc acgggcgcat ccattgagaa atcctcggtg gccaatacgc
      661 tgttgtcgcc acgtggccag gtgaccgtgg atcaacggac caactcgctg ctcattcagg
      721 acgtggccag caagatccgc gaggtgcgca agctgatcgc ccaactggac cagcctgtgc
      781 gccaggtcat gatcgaatcc cgtctggtgg aagcaaccga taatttcagc aaatcactcg
      841 gcgtgcggtt cggcaccaaa tacactgata cctcggccgg gagcggccgg tcaggggcgg
      901 tctccggttc ggtttccgac agctcctcca ttgtctcggc cggaacgctt acggcaaaca
      961 gcagtggtct caatgtcaat ctgccatccg ccggtatcgg cagctccccc gccggagcca
     1021 ttggcatcac ccttgccaag atcggttcca acggcggcct gttgaatctg gagttgtcag
     1081 ctctggagca ggaaggcaag ggcaaggtta tatccagccc acgcgtcatc accgccaacc
     1141 agaaaccggc tcgcatcgag cagggccagg aacgcgtatt caccacgaac gtgctgggcg
     1201 tgggttccgt ggtaaccaaa aaagccacgc tcaagctgga agtcacgccg cagatcacgc
     1261 cggatgaccg cgtcaacatg gaagtgaaca tcacgaagga caatttcgcc gacgccgtga
     1321 ccggcgtgct gaatgtcaag gagatcaaga cgcaggtact gctcgacaac ggcgaaaccg
     1381 tggtcatcgg tggtatctat gaacaggaca aaaatgacac cgttacgaag gtcccgcttt
     1441 ttggggatct tcccctgctg ggctggctgt tcaagagcaa ggaagccaaa gacaacaaga
     1501 ccgagctgtt gattttcctc accccccgga ttctttcaga taatctgagt ctgcgctgaa
     1561 gcatccccgg caaccccacc gccagatggc ggcggggttg cttgctttat agccgcgatg
     1621 ctggcatacc tgcgtcatga gccggctgga caatattttt ctgatcggtc cgatgggggc
     1681 ggggaaatcc accatcgggc gtcatcttgc cgagttgctg aacaaggatt tccgggattc
     1741 cgaccaggaa atcgaaaaac gtaccggggc cggcatcccg ctcatcttcg aaatcgaggg
     1801 cgagaccggt ttccgcagcc gcgaatcgtc cgttctggat gacctgacgc gccaatccaa
     1861 tctggtgctg gccaccggcg gcggtgccat cctgtcgccg gataaccgta aaatcctgca
     1921 tgagcgcgga gtcgtggtgt acctgcacgc accgctggaa acgctgctga gccgcacacg
     1981 ccgtgaccgc caccgtcccc tgctgcagac ggcggaccgg cgccgcacct tggaggatat
     2041 tctcaaggcg cgcgaaccga tttatcgtca ggccgcggac gtggtcatcg aaacctcgca
     2101 ccgctcgccg gtgagtgtgg cgcgcgagat cgttcgcaaa ctggaatcgc attcgaaaaa
     2161 tgaaaacctt gcctcttgat ctcggcgagc gcagctaccc gatctacatc ggttccggcc
     2221 tgctcggcca gaccgagttg ctgaaaaagc atgttgttgg cagccgcgtc gccatcgtga
     2281 ccaatgaaac cgtggcgccg ctttatcttt ccaaggtgcg ctcccacttg agcatgctga
     2341 aaccggtgga agtgattctg ccggatggag agcaatacaa aacacttgaa atattgaacc
     2401 ggattttcga cgctctgctg ggggcgcgtt gcgaccggcg caccacactc atcgcactgg
     2461 gtggcggtgt gatcggcgac atggccggat tcgccgcggc ctgttaccag cgcggcgttc
     2521 cctttatcca ggtgccgacc accttgttgt cgcaggtgga ttcctcggtt ggcggcaaga
     2581 ccggcgtcaa tcatccgctc ggcaagaaca tgatcggcgc gttctatcag ccgcgagcag
     2641 tcatcatcga caccgacaca ctcaacactc tcccggatcg cgagctgtca tccgggctgg
     2701 ctgaagtgat caagtacggc ctgatccgcg accccgagtt tttcgcctgg ctggagacca
     2761 atctggacaa actgctggcg cgtgacccgg aggcgctggc ctttgccatc caccgttcct
     2821 gccgcaacaa ggcggaggtg gtggccgcgg acgaacgcga atccggcgtg cgcgccacgc
     2881 tcaacctggg gcacaccttc gggcacgcga tcgagaccgg tatgggttat ggcgaatggt
     2941 tgcacggcga ggccattgcc gcgggcatga ccatggcggc agacctgtct cggcggctcg
     3001 gatggctgac aacggcggag accacgcgca ttgaaaaact gatgaagcgc gcacgtctgc
     3061 cggtacgcgc gccgggctcg ctgtcgtcgg cgcgctttct cgaactcatg gcggtggata
     3121 agaaggtgtt ggatggccgc ctgcggttgg tgctgctcaa acctctcggc gaggccgtga
     3181 tcaccgacga atatccgcgc accgggctcg aggccacgct cgccagcatg cgcgaagcag
     3241 cctgatgctg gcctcttacg ccgccgccga taaaacctcg cgtgggcgac gctatcccga
     3301 aaccacgccc acgaaccgtt ccgaattcca acgcgaccgc gaccgcatca ttcacagcgc
     3361 cgcgtttcgc cgcctcgaat acaagaccca ggttttcatc aaccacgaag gcgacctgtt
     3421 ccgcacgcgc ctgacgcatt cgattgaggt ggcgcagatc gcgcgctcca tcgcccgcgc
     3481 gctgggactc aacgaggatc tgaccgaggc gatcgcgctg gcgcacgatc tcgggcacac
     3541 acccttcggc cattcgggtc aggatgcatt gaacgaatgt ctgcgcgatt acggcggctt
     3601 cgagcacaat ctgcaatcgc tgcgtgtcgt ggatgtgctg gaagagcgct atgccgaatt
     3661 tccggggctc aatcttacct tcgagacgcg tgagggcatt ctcaagcact gttctctcaa
     3721 gaacgcccgc gagttgggcg aactcgggaa gcggtttctg gaaaaaaccc accccagtct
     3781 ggaggcgcag ctggtcaaca tcgccgacga aattgcctac aacaatcacg acgtggatga
     3841 cggcctgcgt tccggactgc tgacattgga tcagctcaaa cccatcacca tcttcgccgg
     3901 acaatatgat gaggtactgc gacgctaccc agacatccct ccgcgccgcg ccatctatga
     3961 aaccatacgc cgcatgatca accaggtagt caccgacctc atcgagaaca gccggcgccg
     4021 gatcgcgcag tccggtatca aggacatcga ggatgtgcgc caccagcccg agcccctgat
     4081 cgcctacacc acggaggtgg ccgaaaagtc gcgtgagctc aagcgctttt tgcggcagaa
     4141 cctgtacgcg cacgagcgtg tgacccgcat gatgggcgag gcagtgaaaa tcgtgcgcga
     4201 gctgttcgag gctttcatga aggatgcaac gctgctgccg ccccaatatc aggacaagct
     4261 ccggcagcca aacggggccg gcgagcaggc acgcatcgtg gccgattaca ttgccggcat
     4321 gaccgaccgc tatgccatgc gcgaacacac gcggctgttt ggccgttctg gcgcgccgg
//
LOCUS       DBNGZE010000359         2003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1856796, whole genome shotgun sequence.
ACCESSION   DBNGZE010000359 DBNGZE010000000
VERSION     DBNGZE010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1856796"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..496)
                     /locus_tag="AC16FV_09215"
     CDS             complement(134..496)
                     /locus_tag="AC16FV_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA/HybF"
                     /protein_id="HIR2850271.1"
                     /translation="MHELSVCQALISQVEDIARQYHAATIHSISVRIGPLSGVEPDLL
                     LQAFSLASAGTVAQDSRLEIERLPIRVRCESCGMETAAEPNKLVCGACGDWHTRLLSG
                     DEMLLASVELAKTENSYV"
     gene            751..1359
                     /locus_tag="AC16FV_09220"
     CDS             751..1359
                     /locus_tag="AC16FV_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HIR2850272.1"
                     /translation="MKHGPGTLMKNKTLVLGLGNTLLGDEGAGVYAVRALQEQHEDLT
                     DVEFLDGGTLSFTLAGPIEEADNLIVIDATQLQAHPGTVRVFEGEDMDRFLGSHRKSS
                     VHEVSLMDLMAIAHLAESLPARRALIGIQPEYLDWSDAPTDAVARAIPAACDMALQLI
                     NRWRPEQGAGTPRSGDATGREWPPAADNEESPIVIDVRRRRT"
     gene            1356..1808
                     /locus_tag="AC16FV_09225"
     CDS             1356..1808
                     /locus_tag="AC16FV_09225"
                     /inference="COORDINATES: protein motif:HMM:NF016682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation C-terminal
                     domain-containing protein"
                     /protein_id="HIR2850273.1"
                     /translation="MNHLKGIPIAMEGALPATTLSHGNALPLLHEIEILLNDLVTTDK
                     PASIDLRSLPMLPGDYEKLREVLGEGEISATLDVFGPTLVRETAIHGVWWVTHQNADG
                     EVTAEFIEVTYLPEILKTHPADARAAVETLRSRLSQSVPTTEGDDHAG"
     gene            1798..>2003
                     /locus_tag="AC16FV_09230"
     CDS             1798..>2003
                     /locus_tag="AC16FV_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR2850274.1"
                     /translation="MPDNMTLGEKLAQQGISRRTFLKFCASMASLMAMPPTMAAEMAE
                     KLAKARRQSVIWLSFQECTGCTES"
BASE COUNT          411 a          602 c          603 g          387 t
ORIGIN      
        1 gtggcggttg tgcgccgcct gctggtcgtt ggcgctgagc agatttttca gcacggtcac
       61 cgcatcctgg ccctgcgcgt ttttccggcg cgcccgatgt tcgttgccgg gggtgatgtt
      121 gcagccgcag gtttcacaca tagctgttct ccgttttcgc gagctccaca ctcgcgagca
      181 gcatttcgtc tccgctcaaa agcctggtat gccagtcgcc gcaggcgccg cacaccagct
      241 tgttcggctc ggcggcggtt tccatgccgc aggattcgca acgcacgcgg atcggcagcc
      301 gttcgatttc gagccgcgaa tcctgtgcca ccgtgcccgc gcttgccaat gaaaacgcct
      361 gcaacagcaa atccggctcc acgccggaaa gcggaccgat gcgcacgctg atgctatgaa
      421 tggtcgccgc gtggtattgc cgggcgatgt cttcaacctg gctgatcagg gcctgacaga
      481 cggacagttc atgcatgagt ttggtggggc tcggcagctg gttgattcgc catgtgattc
      541 aatcctagcc tgaaaaaacc aatggatttt gacctgtatc aataaacgct aatattccct
      601 gagttacaaa tcacccgtca tttgtttggg agaagcggcg gtacggatac agggtgcaat
      661 tctcgcttga ccatttatat gatgcgcctc accagggcgc tgctgcaagt tctagagata
      721 tatgagcgaa tcgccagggg tgtaaacgac gtgaaacatg gaccaggcac gctaatgaag
      781 aacaagacgc ttgttctggg tttgggcaac acgttactgg gcgatgaggg cgctggcgtt
      841 tacgcggtgc gcgccctgca agagcaacac gaagacctca cggatgtgga gtttctcgac
      901 ggcggcaccc tgagcttcac gcttgccgga ccgatcgaag aagctgacaa tctcatcgtc
      961 atcgatgcca cccagctgca agcgcacccc ggcaccgtgc gtgtgttcga gggagaagac
     1021 atggatcgtt ttctcggcag tcatcgcaaa agcagcgtac acgaggtcag tctcatggac
     1081 ctgatggcga tcgcccacct ggcggagtcg ctgcccgcgc gccgcgcgct catcggcatt
     1141 caacccgaat atctcgactg gtcggacgcc ccgaccgacg cggtcgcgcg cgccattccc
     1201 gcggcctgtg acatggcatt gcaacttatc aaccggtggc ggcccgagca gggagccggg
     1261 accccgcgaa gcggggatgc gaccggccgc gagtggccgc cggccgccga caacgaggaa
     1321 tctccgattg taatagacgt acggaggcgg cggacatgaa ccatctcaag ggcatcccca
     1381 tcgccatgga gggagcgctt cccgctacca ctctaagcca tggcaatgcg ctgccattgc
     1441 tgcacgagat cgagatttta ttgaacgatc tggtgaccac ggacaaaccc gccagcatcg
     1501 atctgcgcag cctgcccatg cttcccggcg attacgaaaa gttgcgcgag gtgcttggtg
     1561 agggcgagat cagcgccacc ctcgacgtgt tcgggccgac gctggtgcgc gagaccgcga
     1621 ttcacggcgt gtggtgggtc acgcaccaga acgccgacgg cgaagtgacc gcggaattca
     1681 tcgaagtgac ctatcttccc gaaatactca agacacatcc cgccgacgcc cgcgcggcgg
     1741 tggaaacgct acgttcacgc ttgtcgcagt cagtgccaac gacggaagga gatgaccatg
     1801 ccggataaca tgactctggg tgaaaaactc gcgcagcagg gaatcagccg ccgaaccttt
     1861 ctcaagttct gcgcctccat ggcctcgctc atggcgatgc cgccgaccat ggctgcggaa
     1921 atggcggaaa agctggccaa ggcccggcgc cagtcggtga tctggctgtc gttccaggaa
     1981 tgcaccggtt gcaccgaatc gat
//
LOCUS       DBNGZE010000360         9476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1648345, whole genome shotgun sequence.
ACCESSION   DBNGZE010000360 DBNGZE010000000
VERSION     DBNGZE010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1648345"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="AC16FV_09235"
     CDS             <1..1675
                     /locus_tag="AC16FV_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2850275.1"
                     /translation="EGDCKNCHARFKKSAQNALCLDCHKDVARDVAQKQGHHGHIPEQ
                     ECRTCHTEHKGRTMNIAPVDEKSFDHKLTDFLLKGGHAGAKVLCRDCHTPKIKFRDAP
                     SDCFACHRKDDTHKGSLGTQCADCHDEMDWKKTRFDHSKTKFPLTGKHRDVICKDCHT
                     DPKFKGAPTACVACHKKDDEHKGRYGTKCESCHGDRDWKATTFNHDRDTKYPLRGEHR
                     LAKCDSCHTGFIYKEKLKTACIACHKADDDKKGHKGRFGEKCQSCHTEKDWGISIFNH
                     DRDTKYPLRGKHLQAKCNSCHTGFLHQEKLMTACVACHKADDDKNGHKGRFGDKCDSC
                     HVEKDWKATTFNHDRDTKYVLHGKHITAKCESCHTGFLYKEKLQTTCIACHEKDDKHK
                     GQEGKKCESCHNEHDWKVAKIDHGLTRFPLLGKHAKVECKKCHLTPAFKDAGIACVGC
                     HEKDDKHKYKLGPRCETCHNALSWESWRFDHDRQTSFKLDGGHRGIACDACHNKPMPR
                     KVTQSSACISCHDVDDVHNGGFGKLCEQCHVTSSFKKIRAGISGVSATH"
     gene            1702..3192
                     /locus_tag="AC16FV_09240"
     CDS             1702..3192
                     /locus_tag="AC16FV_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850276.1"
                     /translation="MTNSANRFRKLSSWIGSLAVLGFALLALPGGDAPAAEARRNVHF
                     DHIKTGFPLTGAHVTLPCETCHVQGTFKGTSKKCETCHTPGSRIQTTTVKPPNHIPTR
                     QACDQCHSSTASWKVARFTHVGVTPHWCGQCHNNSIAPGKPANHPATNQPCDTCHRTT
                     TWMSAAFAHLGVMPGACATCHGSTATRKPSNHIPTTLSCDACHTTGGTFTTSRFTHSA
                     TQGVLPLQCATCHNGAYTQWNALGKHATHIATTASCDTCHTGYVSFAMAGSTFNHTGV
                     APGGCATCHNGSQATGKPSNHIPTTLSCDACHSTGGTFTTSTFTHSATQGVVAGQCAT
                     CHNGAYTQWNALGKPANHVATTASCDTCHKNYTSFAGATFSHTGISPGTCSTCHYQGG
                     PGLGKPTNHIPYESQLLAGASMNCDSCHKSTTSFVTQTMNHNNSLGNGAGWCKGCHQS
                     GASYLGTMQKKSLNHYERYQGQTDCSASGCHRPLGDRGTPYNRWTN"
     gene            3248..3745
                     /locus_tag="AC16FV_09245"
     CDS             3248..3745
                     /locus_tag="AC16FV_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850277.1"
                     /translation="MKNPLLGVTMLLMAFTGSGIASAQEVLLDDVETSVEPEWAVIRV
                     HFTMPVNYVSHFPREHGQLLKIFINVAALDPQDISLRQETRHVSATPVLPAATITFDP
                     PLSLNLQRDPWSLSVRFNRDVNYNVRPGDDNRSIVIYLPVVPVETSPANSPKNTPAAK
                     DKPAK"
     gene            3792..6326
                     /locus_tag="AC16FV_09250"
     CDS             3792..6326
                     /locus_tag="AC16FV_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850278.1"
                     /translation="MRAIPLVALFLLLLNLMPAAIVHAKSEDGGGTGTATDIGTEKHF
                     VVTLRSSFTPISDKPAKRDIPDRPVYLIQSKAFGKNVYNLRVGFFDSFNEASAYRNSV
                     LDKYPAASVTEIGRNEYATIQRAFPVAKPVAPPTEAKKIEPVKALPAVPTPAPAPTPP
                     AATVFSPKALYVVQLEESNQAIRAARSPLPASLKNNRLYVTQTLEKGKTRYQLKLGFF
                     EKEQDVFAARQQLKAAYPNAKVTRVTLNEQNDSVRMALGAAAPVLAAPVVPAAPPARV
                     TVPTVPAAPPAGLPSVAAIPATASDSDALALMDKARSALARGDNSAAVITLDKLLRLP
                     PNRYSQDAQEFIGLARERAREIAAARKEYELYLRLYPTGEGADRVRQRIAALDASITA
                     PSPVLKAAKPRTEAQSSVVGNLSQYYYHGASKVDTTTQATTTNPQNQATLTQTDQSAL
                     VTSLNLTHRYRSEDFDNRFVLRDTHTKNFLTGQDDSNRLSALYYEIKDRKRDYSARLG
                     RQPGSSGGVLGRFDGATAGYNFTLKWRVNVVGGKPTDISYDTDLKFYGTSVDMGPFLE
                     HWGGSLYTMQQKADGVTDRNAVGGELRYFDPGFTVYSLYDYDTEFATSNIILLQGNWT
                     GGSGTSAHVLYDRRKTPSLTLTNALLGETDTSIKSQLQTKTYEQLKQQALDLTATTDL
                     YSVGVSQALSTRWQLGFDLQSAHTSSTVGTVNQPAQPDTGDIYTYTGNVIGTGLFTQR
                     DVSVFSVSHIDAPTYAGNSYSLTNRLLWGTSWSLDTTLSWYSQHDTTTDTKLSRFSPV
                     LRPGYKWKENITFELELGEERTTITSPTTEDKTSRRYWSLGYRWDF"
     gene            complement(6323..6934)
                     /locus_tag="AC16FV_09255"
     CDS             complement(6323..6934)
                     /locus_tag="AC16FV_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR2850279.1"
                     /translation="MTDMRYSRYLTPLVLILGVSFVEPAQAGIYTFTDANGVIHFTNV
                     PSTPRYADMKRVAYLPETAFPRARPVDPDRFSPLVEKAAHEHQIDEALLRAVIAVESG
                     YDPNAVSRKGAVGLMQLMPDTARRYGVRNMYDPAQNIQGGARYLRDLMGKFNNDLPLV
                     LAAYNAGEDAIAQHGNRIPPYRETRTYVPRVMDFYRQYQRGSR"
     gene            complement(7016..7897)
                     /locus_tag="AC16FV_09260"
     CDS             complement(7016..7897)
                     /locus_tag="AC16FV_09260"
                     /inference="COORDINATES: protein motif:HMM:NF048240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850280.1"
                     /translation="MILLAIVALLIAVATAWYLARPLARSVVTDDRERRNQLQQLRDR
                     LLVQLDELDVEEGDRNIDTTVLSDERSRLEAELAAVLHELETPKGKKKKKKTKAESRR
                     GWAVALAIFGVVLPLTAAGLYALNQRPVLAYLSNPQAPPANASMPPMVMEMVARLEKR
                     MAEQPDDADGWHRLGRAYAVLGRGEAANAAYARAYKLTPDDPQLVAEYAAFLYEGDPQ
                     NTSGQVFGLFTRLLELNPENPDALWFLGFAAYQKGDHKKALGYWDRLLKGLPADSPEA
                     EHLRAITAKTRQQLGKK"
     gene            complement(7894..8343)
                     /locus_tag="AC16FV_09265"
     CDS             complement(7894..8343)
                     /locus_tag="AC16FV_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR2850281.1"
                     /translation="MRRLILLMLFAAMPVFAVPVTEDPLERQMLDIAKDLRCAVCQNQ
                     PISESNADLARDMRQIIREQIPAGISREEIINYFVERYGDYVLMNPPVRGPGTVVWLA
                     PVAVLLVVGISGFVFLRHRRKPSLPPAPKLSKEDAALVRAARKQNRA"
     gene            complement(8350..>9476)
                     /locus_tag="AC16FV_09270"
     CDS             complement(8350..>9476)
                     /locus_tag="AC16FV_09270"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR2850282.1"
                     /translation="SLLGTFLVRSGVLSSVQAFAVDPGRGAYILAFMTIVLTVSFGIF
                     MARGRYQQAEEAITSFLSRESLFVWNNVLFLVACASVLLGTLYPLALDALSGQKITVG
                     APYFNIVMVPIFLVILLLMAVGPLVPWRKVNLERLRSRLLWPLLIGVAAAVAMLVFMQ
                     PLHWTAPVAAGLALFALANLITEFARAIRQRRIQLKEPLGRATLKTVLGNRRHYGGMI
                     VHFGIVIIALGLVGSGLFRSELTVTMAPGDVAEIASERLRFEGVEPVRGENYDAMRGR
                     FLLLNSGRSVSPERRQYMRQEMPMTETGIDSTVLRDVYVVLAETSEGGKWAVHVYVNP
                     LVRFIWTGGVIILSGLLLSLSGRRGVRDNETTGNVAQTAE"
BASE COUNT         2125 a         3036 c         2656 g         1659 t
ORIGIN      
        1 cgagggcgat tgcaaaaact gccacgcgcg cttcaagaaa tccgcccaga acgccctgtg
       61 cctggattgc cacaaggatg tcgccaggga cgttgcgcag aaacaagggc atcatggcca
      121 cataccggaa caggaatgtc gcacctgtca tacggagcat aagggacgca cgatgaacat
      181 cgctccggtg gatgaaaaat ccttcgatca caagctcacg gacttcctgc tgaagggcgg
      241 gcatgcgggc gcgaaagttc tgtgccgcga ttgtcacacg cccaagatca aattccgcga
      301 cgcgccgtcg gactgtttcg cctgccacag gaaagacgac actcacaagg ggagtctggg
      361 gacccagtgc gccgactgcc acgatgagat ggactggaag aagacccgtt tcgatcatag
      421 caagacgaaa ttcccgctca cgggcaaaca ccgggatgtc atctgcaagg actgccatac
      481 cgacccgaaa ttcaagggcg ctcccacggc ctgcgtcgcc tgccacaaga aggacgacga
      541 gcacaagggc cggtacggca cgaaatgcga atcctgtcac ggcgaccggg actggaaggc
      601 taccaccttc aatcacgacc gcgacaccaa atatccgctg cgcggggaac accggctagc
      661 gaagtgcgac agctgtcaca ccggatttat atacaaggaa aagctgaaga ccgcctgcat
      721 cgcctgccac aaggccgatg atgacaagaa aggccataag ggtcgtttcg gcgaaaagtg
      781 ccagtcctgc cacacggaaa aggactgggg tatttccatt ttcaaccacg atcgcgacac
      841 caaatacccg ttgcgcggca agcatcttca ggccaaatgc aacagctgcc acaccggctt
      901 cctgcatcag gagaagctca tgaccgcctg tgtcgcctgc cacaaggcgg acgatgacaa
      961 gaatggccac aaggggcgat tcggcgacaa atgcgattcc tgtcacgtcg agaaggattg
     1021 gaaggccact actttcaacc atgaccgcga cacgaaatat gtcctgcacg gcaagcacat
     1081 cacggccaag tgtgaatcct gccacaccgg ttttctatat aaggaaaagc tgcaaacgac
     1141 ctgcatcgcc tgccacgaaa aagacgacaa gcacaagggc caggaaggca aaaaatgcga
     1201 atcctgccac aacgagcatg actggaaagt ggccaagatc gatcacggac tgacccgctt
     1261 cccgctgctg ggcaagcacg ccaaggtcga atgcaagaag tgtcatctga caccggcctt
     1321 caaggacgcc ggcattgcct gtgtcggctg ccacgagaag gacgataaac acaagtacaa
     1381 actgggaccg cgctgcgaaa cctgccataa cgccttgtct tgggagtcct ggcgcttcga
     1441 tcacgaccgg cagacctcgt tcaagctcga cggtggccac cgaggcatcg cctgcgatgc
     1501 ctgccacaac aaacccatgc cgagaaaggt gacgcagagc agcgcctgca tcagttgcca
     1561 cgacgtcgac gacgtgcaca acggcggttt cgggaaattg tgcgagcaat gtcacgtcac
     1621 cagttcattc aagaaaatcc gcgctggtat cagcggtgta tccgcgacgc attgattatc
     1681 tgtcagatga ggagcactac tatgacgaac tcagctaatc gtttccggaa actgtcgtcc
     1741 tggataggct ccctggcggt actgggtttt gccctgcttg cgctgccggg cggtgatgcg
     1801 cccgccgccg aggccagacg caacgtgcat ttcgaccaca tcaaaacggg ttttccgctg
     1861 accggggcgc acgtgacgct tccctgcgag acctgtcacg tacagggcac tttcaaaggc
     1921 acgtcgaaga aatgcgaaac ctgtcacaca cccggcagcc gcattcagac aaccaccgtc
     1981 aaacccccga atcacatacc gacgagacag gcgtgcgatc agtgccattc ctcgaccgcc
     2041 tcgtggaagg tggcgcggtt cactcacgtc ggcgtgacgc cgcactggtg cggacaatgc
     2101 cataacaaca gcatcgcccc gggcaaaccg gcgaatcacc cggccaccaa ccagccttgc
     2161 gacacctgcc accgcaccac cacgtggatg agcgcggcct ttgcccatct cggcgtgatg
     2221 ccgggcgctt gcgccacctg ccatggttca acggcgaccc gtaaaccgtc caatcatatt
     2281 ccgacaacac tttcctgcga tgcgtgccat acaaccggcg gcacgttcac cacctcgaga
     2341 ttcacccact ccgccacgca gggcgtgttg cccctgcaat gcgcgacctg ccacaacggt
     2401 gcctataccc agtggaacgc gctcggcaaa catgcgaccc acatcgccac cacggcctcg
     2461 tgcgacacct gccatacggg ctatgtctca tttgccatgg ccggttccac cttcaatcac
     2521 accggtgtcg ctccgggcgg ttgcgccacc tgccacaacg gctcacaggc caccggcaag
     2581 ccatccaacc acataccgac gaccctgtcg tgtgacgcct gccactcgac cggaggcacg
     2641 ttcacgacct cgacgttcac acactcggct acgcaaggcg tggtggccgg tcagtgcgcc
     2701 acctgtcata acggtgccta cactcaatgg aacgctcttg gcaaaccggc gaatcacgtc
     2761 gccaccacgg cctcgtgcga tacctgtcac aaaaattaca cgagctttgc cggcgccacg
     2821 ttcagccaca cgggcatcag tccgggcacc tgctccacct gtcactacca gggcggtccg
     2881 ggcctgggca agccaaccaa ccatatcccc tatgagtccc agctgctggc cggtgcaagc
     2941 atgaactgcg attcttgcca caagagtacg accagttttg tgactcagac gatgaatcac
     3001 aacaacagtc ttggtaatgg cgctggctgg tgcaaaggct gtcatcagag cggtgccagc
     3061 tatctcggta ccatgcagaa gaagtcattg aaccattatg aacggtacca gggtcaaacg
     3121 gactgttccg cgtccggctg tcaccggccc ctgggtgatc gaggcactcc ctacaatcgc
     3181 tggaccaact agatccggga cgcgtcagaa catgccacaa cgagcctgct tcaaaaggaa
     3241 tgaaatcatg aagaatccgc tactcggggt caccatgctg ctcatggcgt ttaccggctc
     3301 aggcatcgcc agtgcacaag aggtccttct ggatgacgtc gaaacctcgg tcgagcccga
     3361 atgggcggtg attcgcgtcc atttcaccat gccggtcaac tacgtgagcc actttccacg
     3421 ggaacacggg cagttgttga aaatattcat caatgtcgcc gccctagacc cgcaggacat
     3481 cagcctgcga caggaaaccc gtcacgtttc cgccacgccc gtgttgccgg ccgcaaccat
     3541 caccttcgac ccgcccctgt ctctgaattt gcagcgtgac ccgtggtcgc tctcggtgcg
     3601 cttcaatcgc gacgtcaatt acaacgtccg gcccggcgat gacaatcgca gcatcgtgat
     3661 ttatttgccc gtcgtgcccg tggaaaccag cccggcgaat tcgccaaaaa atactccggc
     3721 ggcaaaagac aaaccggcca aataaaaaca cctcccttcc ctacatttaa aattttttga
     3781 atgtaactac catgcgggct atccccctcg ttgcgctgtt tctgttgctt ttaaacctta
     3841 tgccagcggc catcgtgcac gccaaatcgg aggacggcgg cggcactgga accgccaccg
     3901 atatcggcac cgaaaaacat ttcgtggtga cgctgcgcag cagcttcacg cccatttcgg
     3961 acaaaccggc caagcgtgac attccggatc gcccggtata cctgattcaa agcaaggcat
     4021 tcggcaagaa cgtttataac ctgcgcgtcg gatttttcga cagcttcaat gaagccagcg
     4081 cctatcgcaa cagcgtgctc gacaaatatc ccgcggcgtc agtcaccgag atcgggcgca
     4141 atgagtacgc caccattcag cgcgccttcc cggttgcgaa accggtggcg ccccccacgg
     4201 aggccaagaa gattgaaccc gtgaaggcgc taccggcggt gccgacacct gctccagcgc
     4261 cgacaccacc agccgcgact gtcttctcgc ccaaagcgct ttacgtcgtg cagctggagg
     4321 aaagcaatca ggccatacgc gcggcgcgct cgcctctgcc ggcgtcgctg aaaaacaacc
     4381 gtctttatgt cacgcaaaca ctcgaaaagg gcaagacacg ctaccagctg aagctcggct
     4441 tcttcgagaa agaacaggat gtatttgccg cacgccagca attgaaggcg gcttatccga
     4501 atgcaaaagt aacccgtgtc acgctcaatg aacaaaatga ttcggtacgg atggcgctgg
     4561 gcgcagcggc gcctgttctg gccgctcctg tcgttccggc cgcgcctccc gcacgggtaa
     4621 ccgtcccaac cgtcccggct gccccgccgg caggcctgcc gagtgtggcg gcgatcccgg
     4681 cgacagccag cgacagtgat gcgcttgcgt tgatggacaa ggcccgttcg gcgctggcgc
     4741 ggggcgacaa ctctgctgca gtcatcacac tcgacaaact gctgcgattg ccgcccaacc
     4801 gctattcaca ggatgcgcag gagttcatcg ggcttgcgcg cgaacgcgcc agggaaatcg
     4861 cggcggcgcg caaggaatat gaactctacc tgcgacttta tccgaccggc gaaggcgcgg
     4921 atcgggtacg ccaacgcatt gccgcgctcg acgcttcgat caccgcgccc tctcccgtgc
     4981 tcaaggccgc caagccgcgc accgaggcac aatcaagcgt cgtcggaaat ttgtcgcagt
     5041 attactacca tggcgccagc aaagtcgaca ccaccaccca ggcgacgacc accaacccgc
     5101 aaaaccaggc aaccctgacc cagaccgacc aaagcgcgct ggtcaccagt cttaacctga
     5161 cgcaccgcta ccgcagcgag gattttgata accgctttgt gttgcgcgat acccacacca
     5221 agaacttcct caccggtcag gacgatagca accgcctgag cgcgctttat tacgagatca
     5281 aggatcgcaa gcgcgattat tcggcgcgcc ttggacgcca gcccggaagt tccggcggcg
     5341 tactcgggcg ctttgacggc gccaccgcgg gctacaactt cacgctcaag tggcgcgtca
     5401 acgtcgtcgg cggtaaaccg accgatattt cttacgatac cgacctgaaa ttctacggca
     5461 ccagcgtgga catgggcccg ttcttggaac actggggcgg cagcctgtac accatgcagc
     5521 aaaaggccga tggagtcacg gatcgcaacg ccgtgggcgg cgaactgcgc tacttcgacc
     5581 cgggattcac ggtgtacagc ctgtatgact acgataccga gttcgccacc tcgaacatca
     5641 tcctgttgca aggcaactgg accggcggta gcggcacgtc cgcgcatgtg ctgtacgacc
     5701 ggcgcaagac gccatcgctg acgctgacca atgccctgct cggcgaaacc gacacgtcga
     5761 tcaaatcgca gctccagacc aagacctacg agcagctcaa gcagcaggcc ctcgacctga
     5821 cggccacgac cgatttgtac agcgtgggtg tgtctcaagc cctaagcaca cgctggcaat
     5881 tgggtttcga cctgcaatcg gcgcacacct cgagcacggt cgggacggtc aaccagccgg
     5941 cgcaaccgga cactggcgac atctacacgt acacgggaaa cgtgatcggt accggtcttt
     6001 tcacccagcg cgatgtcagc gtgttcagcg tcagccacat cgacgccccg acctacgccg
     6061 gcaattcata ctcccttacc aatcgtcttc tctggggaac aagctggagt ctcgatacca
     6121 ccctgagctg gtacagtcag cacgatacga ccacggacac gaaactgagc cgtttctcgc
     6181 cggtgttgag gccgggttac aaatggaagg agaacatcac gttcgaattg gaactcgggg
     6241 aggaacgcac gaccatcacg agccctacca cggaagacaa gaccagccgg cgctattggt
     6301 cgctcggtta ccgctgggat ttctaacgcg agccgcgctg gtattgacgg taaaagtcca
     6361 tcacccgcgg cacgtaggtg cgcgtctcgc gatagggcgg tatgcggttg ccatgctggg
     6421 cgatggcgtc ttccccggcg ttgtaggcgg ccagcaccaa cggcagatcg ttgttgaatt
     6481 ttcccatcag gtcgcgcaga taacgcgccc cgccctgaat gttctgggcc gggtcgtaca
     6541 tgttcctgac accgtagcgc cgcgcggtat ccggcatcaa ctgcatcagg cccacggcgc
     6601 ccttgcgtga aacggcatta gggtcgtaac ccgactccac cgcgatcacg gcgcgcaaca
     6661 gggcctcatc tatctgatgc tcgtgcgccg ccttttcgac caggggactg aagcgatccg
     6721 gatcgaccgg gcgcgcccga gggaacgccg tttccggcag gtaggccacg cgcttcatgt
     6781 ccgcgtagcg cggggtggac ggcacattgg tgaagtgaat gacgccgttg gcgtcggtaa
     6841 aggtgtatat ccccgcctgg gccggttcga caaaagaaac gccaagaatc aatacgagcg
     6901 gcgtcaggta acgggaatat cgcatgtcgg tcatgccgat gttgttctga tggccatgct
     6961 tataccctag ccctttacca tctgtaaagt ctgccgcccc gccgcccgac gagcgttatt
     7021 ttttgccgag ctgctggcgc gtcttggcgg tgatggcgcg caggtgttcg gcctcggggc
     7081 tgtccgccgg caggcctttc aacaaacggt cccagtagcc aagggctttt ttgtgatcgc
     7141 ccttctgata agccgcgaag ccgagaaacc acaaggcatc gggattttcc ggattgagtt
     7201 cgagcaggcg ggtgaaaaga ccgaatacct gaccgctggt gttctgcgga tcgccttcgt
     7261 agagaaacgc ggcgtactcc gccaccagtt gcggatcatc gggtgtcagc ttgtaggcgc
     7321 gcgcgtaggc cgcgttggcg gcctcgcccc ggcccagcac ggcataggcg cgacccaagc
     7381 ggtgccaacc atcggcatcg tccggttgtt cggccatgcg cttttccagg cgcgcgacca
     7441 tctccatcac catggggggc atggaagcat tggccggcgg cgcctgcgga ttggacagat
     7501 aggccaagac agggcgctgg ttcaacgcat aaagaccggc cgcggtcagc ggcaggacaa
     7561 cgccgaatat ggccagcgca acggcccagc cacggcgcga ttccgccttg gtttttttct
     7621 ttttcttctt tcccttcggt gtttccagtt cgtgcaacac ggcggcaagc tccgcctcga
     7681 ggcggctgcg ctcgtcgcta aggacggtgg tatcgatatt tctgtcccct tcctcgacat
     7741 ccagctcgtc cagctgcacc agcagccggt cgcgcagttg ctgcaactgg ttccgccgtt
     7801 cgcgatcgtc cgtcacaacg ctgcgcgcca gcggccgcgc cagataccag gccgtggcga
     7861 ccgcgatcaa cagggcgacg atggcgagaa gaatcatgcg cggttttgtt tacgcgccgc
     7921 gcgaaccaga gcagcgtctt ccttggaaag ttttggcgcc ggcggcaggg agggtttgcg
     7981 ccggtggcgc aggaacacaa aaccggaaat gccgacgacc agcagcacgg cgaccggcgc
     8041 gagccacacc accgtgcccg gcccgcggac agggggattc atcagcacgt aatcaccata
     8101 gcgctcgaca aaataattga tgatctcctc gcgcgatatg ccggccggga tctgctcgcg
     8161 gatgatctgg cgcatgtcgc gcgccagatc ggcattggat tcggaaatcg gctggttctg
     8221 gcacaccgca cagcgcaggt ccttggcgat gtccagcatc tgccgttcca gcgggtcttc
     8281 cgtcaccggc acggcgaata ccggcatggc ggcaaacagc atcaataaga tcaggcgtcg
     8341 cattgcgttc tactcggcgg tttgcgccac attacccgtt gtttcattgt ctcgcacacc
     8401 tcggcggcca ctgagcgaga gcagcaaacc cgacagaata atcacgccac cggtccagat
     8461 gaaccgcacc agcggattga cgtacacatg caccgcccat ttaccaccct cgctcgtctc
     8521 tgccagcacg acgtacacgt cgcgcaacac ggtggagtcg ataccggtct cggtcatcgg
     8581 catttcctgg cgcatgtact ggcgacgttc cggactgact gagcgcccgc tgttgagcag
     8641 caggaaacgc ccccgcatcg cgtcataatt ctcgccgcgc acgggctcga caccttcaaa
     8701 ccgcagacgc tcgctggcga tttcagcgac atcacccggg gccatggtca cggtcaattc
     8761 ggaacggaac aggcccgagc cgaccagccc gagcgcgatg atgacaatgc caaagtgcac
     8821 aatcatgccg ccgtaatggc gccggttgcc gagcacggtt ttcagggtag cccggcccaa
     8881 gggttccttg agctggatgc ggcgctggcg gatggcgcgt gcaaattcgg tgatcaggtt
     8941 cgccagtgcg aacagcgcca gccccgcggc aaccggcgcg gtccaatgca acggttgcat
     9001 aaacaccagc atcgccaccg cggcggcaac gccgataagc agcggccaca acagacgcga
     9061 ccgcaggcgt tcgagattga ccttgcgcca cggcaccagc ggtcccactg ccatcagcag
     9121 caggatcaca aggaaaatag gcaccatgac gatattaaag tacggcgcgc ccaccgtgat
     9181 cttctggccg ctcagggcgt ccagcgccag tggatacagc gtgccgagca gcacgctggc
     9241 gcaggcaacg aggaacagta cgttgttcca gacgaacaaa gactcgcgcg acaggaacga
     9301 ggtaatcgcc tcttctgcct gctggtaacg accgcgggcc atgaaaatgc cgaaggacac
     9361 cgtcagcacg atcgtcatga acgccagaat ataggcgccg cgccccggat cgacggcgaa
     9421 cgcttgcacc gaggacaaca cgcccgaacg cactaggaag gtgccgagca gtgaca
//
LOCUS       DBNGZE010000361         5627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_260267, whole genome shotgun sequence.
ACCESSION   DBNGZE010000361 DBNGZE010000000
VERSION     DBNGZE010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_260267"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /gene="ruvA"
                     /locus_tag="AC16FV_09275"
     CDS             complement(<1..395)
                     /gene="ruvA"
                     /locus_tag="AC16FV_09275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR2850283.1"
                     /translation="MIGRLHGILLRKEPPALLVDVGGVGYEMEAPMTTFYELPAVGQP
                     VTLHTHLVVREDAHLLYGFAREAQRRLFRDLLKVNGVGPRVALAVLSGLSDEEFMRCV
                     AEEDITRLTKVPGIGRKTTERLVIEMRDK"
     gene            complement(392..928)
                     /gene="ruvC"
                     /locus_tag="AC16FV_09280"
     CDS             complement(392..928)
                     /gene="ruvC"
                     /locus_tag="AC16FV_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014948862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR2850284.1"
                     /translation="MRILGIDPGSRITGFGVVQVLRNGRVEYIASGCVRVPKADLAGR
                     LRTVYDGVAEIIRTYQPTVLAIEKVFMAKNADSALKLGQARGAAILAGANHMLDIAEY
                     TALQIKRAAVGKGHAGKQQVQHMVKALLGLNAAPQADAADALACAICHAHHAVGHLSQ
                     AAAMPRRRSGRRGMRFAL"
     gene            complement(928..1677)
                     /locus_tag="AC16FV_09285"
     CDS             complement(928..1677)
                     /locus_tag="AC16FV_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR2850285.1"
                     /translation="MAGHSKWANIKHRKGVQDARRGKIFTKLIREITVAARMGGGGDP
                     KLNPRLRAAMDKALDANMTKDTIERAIKRGTGELEGAQYEEVHYEGYGPAGVAIMVEC
                     TTDNRTRTVGDVRHAFSKHGGNLGQDGSVAFLFNKTGVLSYPAGTSEDKIMEAALEAG
                     ADDVITDKDGSIEVLTTPEALHAVVEAMTKRGLKPGDAEIQQRATTSAPVMGKDAEEL
                     LKLLEALDDLDDVQSVYSNADIPEALFKQAS"
     gene            complement(2010..2648)
                     /locus_tag="AC16FV_09290"
     CDS             complement(2010..2648)
                     /locus_tag="AC16FV_09290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2850286.1"
                     /translation="MTVTKPQAVVLIHGLWFSGYILFVLARRLQRQGFSVYSFSYPSV
                     RADLSANAARLARFLDTLDADTVHLVGHSLGGILIRALFQDHPRRKPGRIVTLGTPHG
                     GCRVAQHLNRHAFWHRAMGKGVAQLLAGEPQNWTPPREIGVICGTRSFGLGRWLYRGL
                     PRPNDGLLTVKESAFPAAQEHLALPVSHTGMMFSRMVARQIAAFLNAGRFDR"
     gene            complement(2645..3742)
                     /gene="nadA"
                     /locus_tag="AC16FV_09295"
     CDS             complement(2645..3742)
                     /gene="nadA"
                     /locus_tag="AC16FV_09295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR2850287.1"
                     /translation="MATALAYEQFDSIPDGDCDARIREAKARLGKRLVILGHHYQRDE
                     VYRHADFTGDSLKLSRLAASSDADYIVFCGVHFMAEVADILSRPQQISILPDLSAGCS
                     MADMANYTAVQRAWKELGALFNPDQRVTPITYINSSADLKAFCGNHGGLVCTSSNAAR
                     VLEWAYKQREKVLFFPDQHLGRNTAYRMGIPLEDMAEWDFNQPRGGLTREQIEKAKIL
                     LWKGFCSVHQMFKPQHIDAFRKNHPEGKVISHPECSFEVCQKSDYVGSTEFIIKTIEQ
                     SAPGTHWLVGTELNLVNRLAEQGRAQNKVVEFMSPTVCMCSTMFRIDPQHLLWTLENL
                     VVGRVVNQIKVAPAEVEAARVALNRMLDVSP"
     gene            complement(3783..4232)
                     /gene="nudB"
                     /locus_tag="AC16FV_09300"
     CDS             complement(3783..4232)
                     /gene="nudB"
                     /locus_tag="AC16FV_09300"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HIR2850288.1"
                     /translation="MSPSQPTKKFKRPESVLVVVYTMTGKVLLLRRADDGDFWQSVTG
                     SLSWEETEPRQAAVRELREETGLEATGLRDAGLTHRYTILPQWRHRYAPEVKENTEYV
                     YFLALPAEKVITLNSAEHTEYGWYSFDEAASKVFSWTNREAILKAKG"
     gene            complement(4201..>5627)
                     /gene="aspS"
                     /locus_tag="AC16FV_09305"
     CDS             complement(4201..>5627)
                     /gene="aspS"
                     /locus_tag="AC16FV_09305"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR2850289.1"
                     /translation="LRREPMQKNLRLRHKVAQTLRRFLEDRHFTEIETPMLTKSTPEG
                     ARDYLVPSRTHPGHFFALPQSPQLFKQLLMVAGFERYFQITRCFRDEDLRADRQPEFT
                     QLDIETSFLDENGVMDLMEEMLRTLFKNVLGVDLPKPIPRMSYDEAVRRFGSDRPDLR
                     VPLELVDVGDLMKNVEFKVFAGPANNPAGRVAALRVPKGGELPRSQIDGYTAFVGQYG
                     AKGLAYIKVNDAAKGRDGLQSPILKFLPDEVIAGILQRTGAQTGDLIFFGADTAKVVN
                     DALGALRLKLGDDLKLKQDGWKILWVVDFPMFDYDAEAKRWVALHHPFTSPKTEDVER
                     VDLDPANVKARAYDMVLNGSEIGGGSIRIHRVDVQEKVFAALGIGPEEARAKFGFLLD
                     ALRYGAPPHGGIAFGLDRLCALMSGVDSIRDVIAFPKTQKASCLMTEAPSVVDEMQLR
                     ELHIKLRKPPEKTAGEPVPADQKV"
BASE COUNT         1078 a         1740 c         1691 g         1118 t
ORIGIN      
        1 aacttgtcgc gcatttcgat caccaggcgt tcggtggttt tgcgtccgat cccggggacc
       61 ttggtcaggc gcgtgatgtc ttcttccgcc acgcaacgca tgaactcttc atccgacagg
      121 cccgaaagca ccgccagtgc cacgcgcggt ccgacaccat tgaccttgag caggtcgcga
      181 aacaagcgcc gctgagcctc acgcgcgaaa ccatataaca ggtgtgcgtc ttcgcgcacc
      241 accaagtgcg tatgcaatgt gactggctga cccaccgccg gcagttcgta aaaggtggtc
      301 atgggcgctt ccatctcgta acccacgccg cccacgtcca ccagcaacgc cggtggctcc
      361 ttgcgcagca atatgccgtg caaccggccg atcacagggc gaacctcatg ccgcgccggc
      421 ccgaccggcg ccgcggcatg gccgcagcct gcgacagatg tccaaccgcg tgatgcgcat
      481 ggcagatggc gcaggcgagg gcatcggcag cgtcggcctg aggcgcggcg ttgagtccga
      541 gcagcgcctt gaccatgtgc tgcacctgct gcttgccggc atggcccttg ccgacggcgg
      601 cgcgcttgat ctgcagcgcg gtgtattcgg cgatatcgag catgtggtta gcgccggcaa
      661 gaatggccgc gccgcgcgct tgtccgagct tgagcgcgga atcggcgttt tttgccatga
      721 ataccttttc aatcgccagc accgtgggtt gataggttcg gatgatttca gccacgccgt
      781 cgtacaccgt cctgagccgg ccggcgagat cagccttggg cacgcgcacg caaccgctgg
      841 cgatatattc gacccggcca ttgcgcaaca cctgcaccac gccgaagccg gtaatgcggg
      901 aacccggatc gatgccaaga attcgcatca acttgcctgc ttgaacaacg cttcgggaat
      961 gtcggcgtta gagtaaacgc tctgcacatc atccagatca tccagcgctt ccaggagctt
     1021 gagcagttct tccgcgtcct tacccataac gggagcagag gtggtagcgc gctgttgtat
     1081 ctcggcatcc ccgggcttga ggccgcgctt cgtcatggcc tccaccacgg catgaagcgc
     1141 ctccggcgtg gtcaggacct caatcgaacc gtccttgtcc gtgatcacgt catcggcacc
     1201 ggcttcgagc gcggcctcca tgatcttgtc ctcgctcgta ccggcaggat agctgagcac
     1261 gccggtttta ttgaaaagga aggccaccga accgtcctga cccagattgc cgccgtgttt
     1321 ggaaaaggca tggcgcacat cgcccacggt acgggtgcgg ttgtcggtgg tgcactcgac
     1381 catgatggcg accccggccg ggccataacc ttcgtaatgc acttcctcgt attgcgcgcc
     1441 ttcgagttca ccggtgccgc gcttgatggc gcgctcgatg gtgtccttgg tcatattggc
     1501 gtcgagcgcc ttgtccatgg cggcacgcag acgcggattc agtttggggt cgcctccgcc
     1561 gcccatgcgc gcggcgacgg tgatttcgcg gatcagcttg gtgaagattt tgcctcttct
     1621 agcatcctgg acgcctttgc ggtgtttgat attcgcccac ttcgaatgcc cagccatagc
     1681 taagcccttc cttcctctga aatgtgccgc tcaccgcaaa ggcgcgaatt gtgcatgcca
     1741 tccttggcgt cgcgtaggct gcttcgagta accccagccc gaccatccat ggtcgggcgc
     1801 tcagcggcgc gaatgcacgt taagtgcatt cgagacaact ccgcttcgcc ctgcacgccc
     1861 ttgcggtgct tgatattggc ccatttcgaa tggccggcca tgcgagttca gcccccgtaa
     1921 taccgaatta ttcagcgcca gtccgctctg gcagtgggag cgccaagtgt aacatcgggc
     1981 ccgtacatcc cccaaccgcg tgcgcctcct tatcgatcga aacgaccggc attcagaaac
     2041 gcggcaatct ggcgcgccac catgcgtgaa aacatcatgc cggtatgtga caccggcaac
     2101 gccagatgtt cctgcgcagc tggaaaagcg ctttccttca ccgtcagcag gccatcgttc
     2161 ggacgtggca aaccgcgata cagccagcga cccaaaccga aggaacgggt gccacagatc
     2221 acgccgattt cgcgtggagg cgtccaattc tgcggttcac ccgccagcag ttgcgccaca
     2281 cccttcccca tcgcccggtg ccagaaggca tggcggttca ggtgctgtgc cacgcgacaa
     2341 ccgccatgcg gcgtacccag cgtgacgatg cgccccggtt tgcgccgcgg atgatcctga
     2401 aacagtgcgc ggatcaggat gccgcccagg ctgtgtccca ccagatgcac ggtatcggcg
     2461 tcgagcgtat caaggaaacg cgcgagtcgc gcggcattcg cgctcaggtc ggcgcggacg
     2521 gaaggatagg aaaatgaata aacggaaaaa ccctggcgtt gcagtcgccg cgccagcacg
     2581 aacaggatat agcccgagaa ccacaggcca tggatgagga caacggcttg aggtttggtg
     2641 acggttatgg cgaaacgtcg agcatgcgat tgagcgccac gcgcgcggcc tcgacttcgg
     2701 ccggcgcgac cttaatctgg ttgaccacgc gtccgacgac cagattttcc agcgtccaca
     2761 gtagatgctg cgggtcaatg cggaacatgg tggagcacat gcacaccgtg ggcgacatga
     2821 attcaacgac cttgttctgc gcgcggccct gctcggcaag gcggttcacc agattgagct
     2881 cggtgccaac cagccagtgc gtgcccggcg cggattgctc gatggtcttg atgatgaatt
     2941 cggtcgagcc gacataatcc gatttctggc acacctcgaa cgagcattca ggatgcgaga
     3001 tcactttgcc ttccggatgg ttcttccgga aggcatcgat gtgttgcggc ttgaacatct
     3061 ggtgcacgga acagaaacct ttccataaca ggattttggc cttttcgatc tgttcgcggg
     3121 tcagaccgcc gcgcggctgg ttgaaatccc actcggccat gtcttccagt ggaatgccca
     3181 tccgataagc ggtgttgcgc ccgagatgct ggtcggggaa gaacagtact ttctcacgct
     3241 gtttataggc ccattcaagc acgcgcgcgg cgttcgacga agtgcacacg agcccgccat
     3301 gattgccgca gaaggccttg aggtcggccg aggaattgat gtaggtgatg ggcgtcacgc
     3361 gctggtccgg attgaacaac gcgccgagtt ccttccacgc gcgctgcacc gcggtgtaat
     3421 tcgccatgtc ggccatggaa catccggcgg agagatcggg caggatggaa atctgttgcg
     3481 ggcgcgaaag aatgtccgcg acctcggcca tgaagtgcac gccgcagaat acgatgtaat
     3541 cggcgtcact cgaggccgcc aggcgtgaga gcttgagcga gtcgccggtg aaatcggcgt
     3601 ggcggtatac ctcgtcgcgc tgatagtgat gcccgaggat tacgaggcgc ttgccaagcc
     3661 gcgctttcgc ttcgcgaatg cgcgcgtcgc agtccccgtc gggaatgctg tcgaactgtt
     3721 cataggccag tgcggtcgcc atgttatcta acctcaagac gagcgaagca aggctacatt
     3781 aattagcctt tcgctttcag gatggcctcg cggttcgtcc atgaaaaaac tttcgacgca
     3841 gcttcatcga aagaatacca gccgtattcc gtatgctcgg cagaattcaa agttataacc
     3901 ttttccgcgg ggagcgcaag gaaatacaca tattctgtat tctctttcac ctcgggcgca
     3961 tagcggtgcc gccattgcgg cagaatcgtg tagcgatggg ttaatccggc atcccgcagg
     4021 ccggtggcct ccagacccgt ttcctctctc agctcgcgta cggcggcctg gcgcggctcg
     4081 gtctcctccc acgacaggct gccggtcacc gactgccaga aatcgccgtc gtctgcgcgc
     4141 cttaacaaaa gtaccttccc ggtcatggta tacaccacga ccagcaccga ctccgggcgc
     4201 ttaaactttt tggtcggctg ggacgggctc accggcggtt ttttccgggg gcttgcgtag
     4261 cttgatatgc agttcgcgca attgcatctc gtccaccacg ctcggcgcct cggtcatgag
     4321 gcacgaggct ttctgggtct tggggaaggc aattacgtcg cggatggaat ccacgccgct
     4381 catgagcgcg cacaggcggt cgaggccgaa ggcgatgccg ccgtgcggcg gcgcgccgta
     4441 acgcagcgca tccagcagaa agccgaattt ggcgcgcgcc tcctccggac cgatgccgag
     4501 cgcggcgaac accttctcct gcacgtccac acggtgaata cggatggagc cgccacctat
     4561 ctcggaaccg ttcagcacca tgtcataggc gcgcgccttc acgttcgcgg gatcaagatc
     4621 cacgcgttct acatcttcgg tcttgggcga tgtgaacggg tgatgcagcg cgacccagcg
     4681 cttggcctcg gcgtcgtaat cgaacatcgg gaaatccacc acccacagga ttttccagcc
     4741 atcctgtttg agcttgagat cgtcgccgag cttcaggcgc aacgcgccga gcgcgtcgtt
     4801 gaccaccttg gcggtatcgg caccgaagaa aatcagatcg ccggtctgcg cgccggtacg
     4861 ttgcaggatg cccgcgatga cctcatccgg caggaatttc aggatcggcg attgcaaccc
     4921 gtcacgacct ttggcggcgt cgttgacctt gatataagcc aggcccttgg cgccgtactg
     4981 gccgacaaac gcggtgtaac cgtcaatctg gctgcgcggc agttcgcccc ccttgggcac
     5041 gcgcagcgcc gctacgcggc cggccggatt attcgccggc ccggcgaaga ctttgaattc
     5101 gacgttcttc atcagatcgc cgacgtcgac cagttccagt ggcacgcgca ggtcaggccg
     5161 gtcggagccg aagcggcgca cggcctcgtc gtaactcatg cgcggaatgg gcttgggcag
     5221 atccacgccg agcacattct tgaacagcgt gcgcagcatt tcttccatca ggtccatcac
     5281 gccgttttca tcgaggaatg aggtctcgat gtcgagctgg gtaaattccg gctggcggtc
     5341 ggcgcgcagg tcttcgtcgc ggaaacagcg cgtgatctgg aaatagcgtt cgaagcccgc
     5401 gaccatgagc aactgcttga acaactgcgg cgactgcggc aatgcgaaga agtgcccggg
     5461 atgcgtgcgc gagggcacga gatagtcacg cgcgccttct ggcgtggact tggtcagcat
     5521 cggagtttcg atttcggtga aatggcggtc ttccaggaaa cggcgcaggg tctgggccac
     5581 cttgtggcgc aggcgcaggt tcttctgcat cggctcgcgc cgcagat
//
LOCUS       DBNGZE010000362         2439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_260296, whole genome shotgun sequence.
ACCESSION   DBNGZE010000362 DBNGZE010000000
VERSION     DBNGZE010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_260296"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16FV_09310"
     CDS             complement(<1..484)
                     /locus_tag="AC16FV_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2850290.1"
                     /translation="MSDGQTIFVVDDDETVRGSLHWLLESLRLKVQTYASAQDFLSAY
                     DPVQPGCLVLDVRMPDISGLQLQEILAQRQIRIPVILITGHGDISMAVRAMKAGAFDF
                     IKKPFNDQVLLERIQQGLAIDARFRYEAAEREKNIERLGSLTPRESEVLDGVVGGKSN
                     K"
     gene            complement(481..>2439)
                     /locus_tag="AC16FV_09315"
     CDS             complement(481..>2439)
                     /locus_tag="AC16FV_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR2850291.1"
                     /translation="AKVRGTPDALAQRVASALQTMPMEHPAARAGGYAGWTALLDYQP
                     VHELLKDLHLSPYADAENIAIADILHQYTRWVIAGIAFLFLSAGFTAFMLRLNRRLKR
                     AKLFLENEIAVRKRAQQELRRSESALRALHDITSRHSLSFEEKTQALLALGRQQFGLP
                     VGILSRVEGEKYEIVEVVPADGPIPKGCVFPLGRTYCCITLQSREPVSFEHAAESEWR
                     THPAYLQHKLEAYLGIRVEVEGGVYGTLNFVSPEPRAVLFTNSDKEILKLMAQWMGRE
                     IERNRAHAQMQKLSSALEQTADAVMIVSRGGLIEYVNPAFEKVTGYTRGEAVGKNPNI
                     LRSDRHGEEFYRQLWKTILHGESFHDTFINRRQDGMLYYEEKTITPIKDVHGVVTHFI
                     ATGKDVTKRKLAEERALQRQAQLTHVQRVSAMGAMATSLAHELNQPLAAIVNYARGCI
                     HRLRGGELRSDELRTALEHIATEGARSGEIIQRVREFLRKGKPLRTRADINQIVREAA
                     DLAGPEARRKDIALRLELTEGLPLVLVDAVQIEQVVLNLVHNAIEAIDEAQCQRREVT
                     VRTGPDPKNGIEVIVRDTGPGLPLKNFDRLFEPFFSTKPVGMGMGLSISRSIIEAHGD
                     QLSAVANIDGGAIFHFTLPAAVEGGKLQ"
BASE COUNT          438 a          763 c          746 g          492 t
ORIGIN      
        1 gcttgttgga cttgccaccg accacgccat ccagtacttc actctcacgc ggagtcaacg
       61 aaccgagacg ctcgatattt ttttcccgct ctgcggcttc atagcgaaag cgagcgtcta
      121 tcgccaagcc ctgttgtatc cgttccagca gcacctgatc gttaaagggt ttcttgatga
      181 aatcgaaagc gcccgctttc atggcgcgca ccgccatgga gatatcgccg tgccctgtga
      241 tcaggatgac ggggatgcgg atttgccgct gcgccagaat ctcctgcagc tgcaacccgc
      301 tgatgtccgg catgcgcacg tcgagcacca gacagccggg ctggaccgga tcgtaggcgg
      361 acaggaaatc ctgtgccgag gcataagtct gcaccttgag cctcagtgac tcgagcagcc
      421 agtgcagcga gccgcgtacc gtctcgtcat catccacgac gaaaatggtt tgcccatcgc
      481 tcattgcagc ttgcctccct ccaccgccgc aggcagggta aaatgaaaaa tcgcgccgcc
      541 atcgatgttc gcaaccgccg aaagctggtc gccatgcgct tcgataatcg agcggctgat
      601 ggacaggccc atacccatgc cgaccggttt ggtggaaaag aacggctcaa agaggcgatc
      661 gaaattcttc agcggaaggc ccgggccggt gtcgcgcacg atcacctcaa tcccgttctt
      721 cggatcgggg ccggtccgca cggtcacctc gcgccgctgg cattgcgcct cgtcaatggc
      781 ctcgatggcg ttgtgcacca ggttcagcac cacctgctcg atctgaaccg cgtccacgag
      841 cacgaggggc aaaccttcgg tcagctcgag ccgaagcgcg atgtccttcc gtcgtgcttc
      901 cgggcccgcc agatcggccg cctcgcgcac gatctggttg atgtccgcgc gtgtgcgcag
      961 cggcttgcct ttgcgcagaa attcccgtac ccgctggatg atctcgcccg accgggcgcc
     1021 ttcggtggcg atgtgttcga gcgccgtccg gagttcgtcc gagcgcagct cgccgccgcg
     1081 caggcggtgg atgcatcccc gggcgtagtt aacgatggcg gccaggggct ggttgagttc
     1141 atgcgccagc gacgtcgcca tcgcccccat ggcgctgacc cgctgcacgt gcgtcagctg
     1201 cgcctggcgc tgcagtgcgc gttcctcggc cagcttgcgt tttgtcacgt ccttgccggt
     1261 ggcaatgaag tgcgtcacga cgccgtgcac gtccttgatc ggggtaatgg tcttttcctc
     1321 ataatacagc atgccgtcct gacgccggtt tatgaaggtg tcgtgaaacg actcgccgtg
     1381 cagaatggtt ttccacagct ggcgatagaa ctcttcgccg tgccggtcgg accggaggat
     1441 gtttggattc ttgccaacgg cctcaccccg ggtatagccg gtgaccttct caaatgcagg
     1501 attgacgtat tcgatgaggc caccgcgact gacgatcatg acggcgtcgg cggtctgctc
     1561 cagcgcgctc gagagcttct gcatctgggc gtgggcgcga tttcgctcga tctcgcgccc
     1621 catccactgc gccatcagtt ttaagatctc cttgtcgcta ttcgtgaaca gcacggcgcg
     1681 cggctcgggg ctgacgaaat tgagggtgcc gtacaccccg ccttcgacct ccacgcggat
     1741 gccaaggtag gcctccagtt tgtgctgaag atacgcggga tgggtgcgcc attccgactc
     1801 cgcggcatgt tcgaaactca ccggttcgcg ggattgcagc gtgatgcagc aataggtacg
     1861 gccgagaggg aagacgcagc ctttgggaat cggaccatcc gccggcacca cctccacgat
     1921 ctcgtacttt tcgccctcaa cgcgggaaag gataccgacg ggcaggccaa attgctggcg
     1981 gcccagcgcg agcaacgcct gtgttttttc ttcaaaagag aggctgtgcc tagacgtgat
     2041 gtcgtgcagc gcgcgcagcg cgctctcact gcgccgcaac tcctgctgcg cccgtttccg
     2101 cacggcgatt tcgttttcca gaaaaagttt cgcgcgcttg aggcgacggt tcagccgcag
     2161 cataaatgcg gtgaaaccgg ccgataaaaa cagaaaggcg atgcccgcga taacccagcg
     2221 tgtgtactga tggaggatat ccgcgatggc gatgttctct gcgtccgcgt acggggagag
     2281 gtgcaggtcc ttgagaagtt catgtaccgg ctggtagtcg aggagagcgg tccagccggc
     2341 gtacccgccc gcgcgggcgg cgggatgttc catcggcatg gtttgcagcg cgctggccac
     2401 gcgttgcgcc agcgcgtccg gcgtgccgcg caccttggc
//
LOCUS       DBNGZE010000363         5262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_120519, whole genome shotgun sequence.
ACCESSION   DBNGZE010000363 DBNGZE010000000
VERSION     DBNGZE010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_120519"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC16FV_09320"
     CDS             <1..789
                     /locus_tag="AC16FV_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2850292.1"
                     /translation="RHDKDLLACVRNKDMVSRTLSGTGDRRLILPIFGVGNITAMLVI
                     EGMHDVGGEDEVLARLLQIFSNQLYLLSRNELDGLTGLHNRQSFDERIKQLVLSAGHD
                     ERRTHQFVAAQHNSLALLDVDHFKMVNDKYGHLYGDEVLVQFSRLMSRSFRHQDMMFR
                     YGGEEFALVLVGMTPEAAVPMLDSFRGALEAYAFPQIGCKTVSIGVAGISPGESVDTI
                     INRADKALYYVKQNGRNQVCCYETLVAEGKLAPVTVATGDIELF"
     gene            887..1105
                     /gene="thiS"
                     /locus_tag="AC16FV_09325"
     CDS             887..1105
                     /gene="thiS"
                     /locus_tag="AC16FV_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR2850293.1"
                     /translation="MKIIINGQEKSFEAPVTITALVQEMGLSERRIAVEVNREIVPRS
                     RHGEFELKDNDRVEVVFAIGGGRNGGNR"
     gene            1089..1901
                     /locus_tag="AC16FV_09330"
                     /pseudo
     CDS             1089..1901
                     /locus_tag="AC16FV_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233047.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
     gene            2007..2699
                     /gene="trmB"
                     /locus_tag="AC16FV_09335"
     CDS             2007..2699
                     /gene="trmB"
                     /locus_tag="AC16FV_09335"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR2850294.1"
                     /translation="MVDSSKALRSIRSYVRRESRITPAQVRALGELWPRYGIPEGTAL
                     LDWPTIFGRAAPVILEIGFGNGETLAATATSHPENDYLGIEVHRPGAGSLLRRLEAQG
                     LRNVRVMLGDAGEILAQRVADASLTAVHLFFPDPWPKKRHHKRRLVQPDFSALVAHKL
                     ALGGCFHLATDWPAYAEHMLTVLSHTEGLVDASGTAQFQKLVAERLSTRFEQRGRKLG
                     HEVRDLVYLRKA"
     gene            complement(2700..3023)
                     /locus_tag="AC16FV_09340"
     CDS             complement(2700..3023)
                     /locus_tag="AC16FV_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2850295.1"
                     /translation="MSQDDRPEANRDGNAAAAEQPWFVYILECSDGSFYTGITNNLER
                     RQQQHNDGTASRYTRSRRPVILRYQEVCESRSQALIRECSLRLLTRKQKEDLVNRGRL
                     GGDAA"
     gene            complement(3033..3866)
                     /locus_tag="AC16FV_09345"
     CDS             complement(3033..3866)
                     /locus_tag="AC16FV_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR2850296.1"
                     /translation="MHERLAKIAKISEQELNLAEAALLIAVEEYPGLDVEAYLRQIDE
                     LATGVQERLPAQAGLEDTLVTLNQFLFVEQGFSGDTETYDDPRNSFLNEVLDRKRGIP
                     ITLSIIYMEVGRRLGLPLKGVSFPGHFLVKFSTREGEVVLDPFSGGSLLSKEDLEEIL
                     EETYGPAESVNAAPLERLLTAAGKKDILVRMLRNLKGAYLRREQFDKALTVVDRILVI
                     QPDQPDEVRDRGRIYEQLECFRAALENYQSYLSLRPSSGDAQDVHKRIAELKQIVANL
                     N"
     gene            complement(4040..4288)
                     /locus_tag="AC16FV_09350"
     CDS             complement(4040..4288)
                     /locus_tag="AC16FV_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR2850297.1"
                     /translation="MAKILYFSILVDKFGRAEEEVSLPGSVTDVRTLLAWLRTRGDSW
                     EKALKEDAVKVTLNRQFAEVDTAVNDKMEIAIVPARLG"
     gene            complement(4349..>5262)
                     /locus_tag="AC16FV_09355"
     CDS             complement(4349..>5262)
                     /locus_tag="AC16FV_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2850298.1"
                     /translation="AWLSTAAAQTCGTAAPVQVQVLGSGGPELQDRRASSSYLVWIDG
                     KPRALVDIGGGAALRFGESGATVSDLDVILLTHLHADHTADLPALVKSSFFEDRPRPL
                     PIYGPDGNQFMPSTVTFVRTLFDSVRGSYRYLGDFLNPLARGGYKLQPQDVRPLSGKL
                     KAGRQPGDEIVYPFANNRLYASAIPVTHGNIPALAWRVTAGGKGMVFSGDTNGRGEQL
                     ARLAQGADLLVAHNAVPEGASGVERDLHMPPSVIGAIAQQAQVKQLVLSHRMLRTLGK
                     EEETLAAIRQRYAGPVQFANDLDCFTP"
BASE COUNT         1005 a         1538 c         1667 g         1052 t
ORIGIN      
        1 aggcacgaca aggacctgct ggcctgcgtg cgcaacaagg acatggttag tcgcactctt
       61 tccggtacag gagaccgccg tctgattctg ccgatattcg gtgtgggcaa tatcaccgcg
      121 atgctggtga tcgaggggat gcacgatgtt ggcggtgaag acgaggtgct cgcgaggctc
      181 ctgcagatat tcagtaacca gctgtatctg ctttcgcgca acgagctgga cggactgacc
      241 ggtttgcata accgtcagtc attcgatgag cgcattaaac agctggtgct tagtgcgggg
      301 catgatgagc ggcgcacgca ccaattcgtt gcggcgcaac ataacagcct ggccctgctt
      361 gatgtcgatc acttcaaaat ggtgaatgac aagtacgggc acctgtatgg cgatgaagtg
      421 ctggtgcaat tctcgcggct catgagtcgt tcgtttcggc atcaggacat gatgttccgt
      481 tatggcggcg aggagtttgc cttggtcctg gtcggcatga cccccgaggc tgccgtgccc
      541 atgctggatt cttttcgagg ggcattggag gcttacgcgt ttccacaaat tggttgcaag
      601 actgtgagta tcggtgtggc gggtatctcc cccggtgaga gtgtagatac gatcattaac
      661 cgggctgaca aagcgctgta ctacgtcaag cagaacggcc gcaatcaggt ctgctgctac
      721 gagacactgg tggcggaagg aaagcttgcc ccggtgacgg tggccacggg cgacatcgag
      781 ttgttttgat cgcacgccat tccggcgtgc gttcttccat agctgggatt acaatatctg
      841 cgccggtagc gccggaacgc gtatttctgg atgtcatccc cagtccatga agatcataat
      901 caacggtcag gaaaagagct tcgaggcccc ggtgacgatc accgcgttgg tgcaggaaat
      961 gggcctctcg gaacggcgga tcgccgtcga ggtcaaccgc gaaattgtcc cgcgcagccg
     1021 gcacggcgag tttgaattga aggacaatga tcgcgtcgaa gttgtgtttg ccattggggg
     1081 cggaagaaat ggcggtaacc gctgaaacca tccgtaaaac gaccaacgat ccgctggtga
     1141 tcgccggccg ggcatacttg tcgcgcttgc tggtgggcac gggcaagtac aaggatctcg
     1201 aggaaacccg gcgtgccgtg gaggcgagcg gggcgcagat catcaccatc gccgtgcggc
     1261 gcaccaacat cggtcagaac ccgaacgagc cgagcctgac tgacgtgctg ccgccgacga
     1321 aatataccta tctgcccaac accgccggct gttacagcgc cgaggacgcc atccgcacct
     1381 gccgtctcgc gcgctagttg ctcgacggcc atggcctcgt gaagctggag gtcctgggca
     1441 gtgaaaagac gctttatccc gatattcccc agacactggc ggcagccgaa accctgatca
     1501 gagacggctt caaggtcatg gtatacacca atgacgatcc gatcgtggcc cgcaaattcg
     1561 ctgagatggg ctgcgtcgcg gtcatgccgc tcgccgcgcc gatcggatcg gggctgggca
     1621 tccgcaatcc ctacaacatc cgcttcatcg tggaagaggc caccgtgccg atcctggtgg
     1681 atgccggtgt cggcaccgcc agcgacgccg ccatcgccat ggaactcggc tgcgacggcg
     1741 tgctgatgaa caccgccatc gccggcgcgc agaacccggt gctcatggcc caagccatga
     1801 agaaagcggt cgaggccgga cgtgaggcct ttctcgccgg ccgcatgccg cgcaagcagt
     1861 acggctcgcc gtcgtcgcct ctcggcggca cgtttttcta ataatcttga accgccaaga
     1921 cgccaaggac gccaagaaaa gcagaatcaa atttcagaaa tcttggcgct cttggcgctc
     1981 ttggcgcctt ggcggtttgc ttttaaatgg tcgactctag caaggcgtta agatccatcc
     2041 gcagctacgt gcgtcgcgag agccgcatta cgccggcgca ggtgcgcgcg ctcggtgaac
     2101 tctggccgcg ctacggcatc ccggaaggaa ccgcgctgct ggattggcca actatcttcg
     2161 ggcgcgccgc gccggtgatt ctcgaaatcg gttttggcaa tggcgaaaca ctggccgcca
     2221 cggctacctc tcatccggaa aacgattatc tgggcatcga ggtgcaccgc cccggcgcgg
     2281 gcagtctgct acgccggctg gaggcgcagg gcctgcgcaa cgtgcgcgtg atgctgggcg
     2341 acgccggcga aatcctggcg caacgggttg cggatgcgtc cctaaccgcc gtgcatcttt
     2401 ttttccccga cccgtggccg aagaagcggc atcacaaacg ccgtctggtg cagccggatt
     2461 tctccgcgtt ggtggcgcac aagctcgcgt tgggcggctg ttttcatctg gcgacggatt
     2521 ggccggcgta tgcggaacac atgttgacgg tactgtcgca caccgaggga ttggtggatg
     2581 cgagcgggac cgcgcagttt cagaagctgg tggcggagcg tttatcgacc cgctttgagc
     2641 agcgcgggcg caagctgggc catgaggtac gggacctggt gtatctacga aaggcctgat
     2701 caggctgcgt cgccgcccaa tctcccccgg ttcacgaggt cttctttttg cttgcgcgtc
     2761 agcaggcgca gcgaacactc gcggatcagc gcctgggagc ggctttcgca gacctcctga
     2821 taacgcagga tcaccggccg gcgactgcgc gtgtagcgcg aagcggtgcc gtcgttgtgc
     2881 tgctgctggc gccgttcgag attgttggtg atgccggtgt agaacgagcc gtcgctgcat
     2941 tccaggatgt acacaaacca cggctgttcc gcggcggcag cgttgccatc ccggttggct
     3001 tcgggtcggt catcctgtga catgatagaa acctagttga ggttggcgac gatctgtttc
     3061 agttcggcga tgcgcttgtg cacgtcctgt gcgtcgcctg aacttggtct caggctgagg
     3121 taggactgat aattttccag cgcggcgcgg aagcactcca gctgctcgta gatacgaccg
     3181 cgatcgcgca cttcgtcggg ctggtccggc tggattacca ggatacggtc cacgaccgtg
     3241 agcgccttgt cgaactgctc gcgccgcagg taggcgccct tgagattgcg cagcatgcgc
     3301 accaggatgt ctttcttgcc ggccgcggtc agcaggcgtt cgagcggcgc ggcattgacg
     3361 gattccgccg ggccataggt ctcctcgagt atctcctcga ggtcttcctt gctcagcaga
     3421 ctgccgccgg agaacgggtc gagcacgacc tcgccttcgc gggtcgagaa tttaaccaga
     3481 aagtggccgg ggaaggacac gcccttgagc ggcaggccca ggcgccgtcc gacctccatg
     3541 taaatgatgg agagcgtgat ggggatgccg cgcttgcggt ccagcacctc attcagaaag
     3601 ctgttgcgcg ggtcgtcgta agtctcggtg tcgccggaaa agccctgttc cacgaacagg
     3661 aactggttga gggtcaccaa ggtgtcctcc aggccggcct gcgcgggcag acgctcctgc
     3721 acgccggtgg cgagctcatc gatctgccgc aggtaggcct cgacgtccag accggggtat
     3781 tcctcgaccg cgataagcaa cgccgcttcc gccagattca gctcctgctc ggagattttg
     3841 gcgattttgg cgagacgttc gtgcatgcgt gattccatat tcacccgtcc ggtgacggcg
     3901 tggttccgta attctctagt taaggatagc agacggttga aagggcgcgc ggcaccaacg
     3961 gttctggaga aacacaaggc cattcaggga gttgaacggg atttttggtg tgaaaccgaa
     4021 aatgaaaatt tgccggaggc tatccaaggc gtgccggaac gatggcgatt tccattttgt
     4081 cattgaccgc cgtgtcgact tcggcgaact ggcggttgag cgtgactttc acggcatctt
     4141 ccttgagtgc tttttcccag ctatcaccgc gcgtccgcag ccatgccagg agcgtgcgca
     4201 cgtcggttac cgaccccggc aggctgacct cttcttcggc gcggccgaat ttgtccacaa
     4261 gtattgaaaa ataaaggatt tttgccatta caggggtcgt ggatatcaat attggcagag
     4321 tagtcagacg cctattttag acgctctgtc agggggtaaa acagtcaaga tcgttggcga
     4381 attggaccgg cccggcatag cgctggcgta tggcggccag cgtttcctcc tctttcccga
     4441 gcgtgcgcag catgcgatgt gacagcacca gttgtttgac ctgggcttgt tgcgctatcg
     4501 ctccaattac tgaaggcggc atgtgcaggt cacgctcgac gccgctggct ccctcgggca
     4561 cggcgttgtg cgcgaccagc aggtcggcgc cctgcgccag gcgcgcgagc tgttcaccgc
     4621 gaccattagt gtcgccgctg aaaaccatgc cttttccacc cgccgtaacg cgccaagcca
     4681 gcgccgggat gtttccgtgg gtcacgggaa tagcactggc atacagcctg ttattggcaa
     4741 acggatacac gatctcatcg cccggctgac ggccggcctt gagcttgccc gacagcggac
     4801 gcacgtcctg cggctggagt ttgtagccgc cgcgcgccag cggattcaaa aaatcgccga
     4861 gatagcggta ggaaccgcgc acgctgtcga acagagtgcg cacgaacgtg accgtggacg
     4921 gcatgaactg gtttccgtca ggaccgtaga tcggcagcgg ccgcgggcgg tcctcgaaga
     4981 acgacgattt gaccagcgcc gggaggtcgg ctgtgtgatc ggcgtgcaag tgcgtaagca
     5041 ggatgacgtc gagatccgac accgtggccc cggattcgcc gaagcgcagt gccgcaccgc
     5101 cgccgatatc caccagtgcc cgcggcttgc cgtcgatcca cacgagataa ctgctggagg
     5161 cgcgccggtc ctgcaactcc ggaccgcccg agcccagcac ctggacctga actggcgcgg
     5221 cggtgccgca ggtctgcgcc gctgcggtcg aaagccacgc ga
//
LOCUS       DBNGZE010000364         4820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_120535, whole genome shotgun sequence.
ACCESSION   DBNGZE010000364 DBNGZE010000000
VERSION     DBNGZE010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_120535"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2238)
                     /locus_tag="AC16FV_09360"
     CDS             complement(<1..2238)
                     /locus_tag="AC16FV_09360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR2850299.1"
                     /translation="MKEWNLQKRVLFLALAPLTVAVLLLSLHFTSARIQDLEDALRNR
                     GQAIATQLAAISEDAFLSGNRKKLARFVQATLQEKNVLSVTVTDMKGDILAHAAEAMD
                     KNKPPPLAESSRYKLLAPVYYSEETGEAKQNKESAAVPKKQAGWVTVQLSSAALQTEQ
                     NKILFQSLFIVLVGLVATVVFALLMARGVTRPVISLTRAVEKIKNGVLDYRVSMQSGG
                     EVGRLEDGINAMAAALQEARDREKKRSEDALFLEKVRAQVTLESIGDGVITTDATGRI
                     VYMNPVAEQFTEWRGDLAQGRPLSEVFKIFDEESNRLEEYPIHYCLREGRTIRHDSHH
                     LLMSHEGGKIEIQDSAAPIRDRDGVILGAVVVFHDVTEIQSMARHMAFLASRDPLTGL
                     LNRREFESRLQHVLESARAGERTHALLYLDLDQFKIVNDTCGHVAGDELLKQLASHLQ
                     KEIRASDMLARLGGDEFGVILEDCSIDKAEQIADLLRQSVKDFRFMWEKRAFEIGVSI
                     GLVPISRDSSGLTEVLSAADSACYIAKDRGRNRIHIYQPDDRAFAQRRGEMQWVHRLR
                     QGLENNSFDLYCQAIVPLRDGKQQPGTFHEILVRVQDEDLVLPAAFIPAAERYHLMPS
                     IDRWVIRTVFHMLEKYQAQTGKGEGTGESLFAINLSGQSLGDEQFLEFVMQQFAQHRV
                     DPRSICFEITETAAIANLTRARDFIARFKHMGCRFALDDFGSGLSSFGYLKSLTVDYL
                     KIAGDF"
     gene            complement(2246..3049)
                     /locus_tag="AC16FV_09365"
     CDS             complement(2246..3049)
                     /locus_tag="AC16FV_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR2850300.1"
                     /translation="MIVLFTEFGLTDPYVGQMHAVLAREAPGVPVIDLFHSVPAFNIR
                     AGAYLLPAYARDFPPGTVFICVVDPGVGGARRPVMLQADKRWYVGPDNGLFEMVMRRA
                     TEYEYRVIQWRPARLSASFHGRDLFAPVAAKLIRGEIPDSEPAGYFPERRSTESLRAR
                     GEIPDAASDELTAPADEIWPDDLAEIIYIDHYGNGITGLRKASVSNTQAIRVGGEVLK
                     YARVFSDVPPGTAFWYENANGLVEIAVNGGSAAARLGFRPGDPLTLMQP"
     gene            complement(3292..3666)
                     /gene="rplQ"
                     /locus_tag="AC16FV_09370"
                     /pseudo
     CDS             complement(3292..3666)
                     /gene="rplQ"
                     /locus_tag="AC16FV_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(3689..4702)
                     /locus_tag="AC16FV_09375"
                     /pseudo
     CDS             complement(3689..4702)
                     /locus_tag="AC16FV_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            complement(4732..>4820)
                     /gene="rpsD"
                     /locus_tag="AC16FV_09380"
     CDS             complement(4732..>4820)
                     /gene="rpsD"
                     /locus_tag="AC16FV_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139892.1"
                     /note="primary rRNA binding protein; nucleates 30S
                     assembly; involved in translational accuracy with proteins
                     S5 and S12; interacts with protein S5; involved in
                     autogeneously regulating ribosomal proteins by binding to
                     pseudoknot structures in the polycistronic mRNA; interacts
                     with transcription complex and functions similar to
                     protein NusA in antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR2850301.1"
                     /translation="KGTFKSVPERNDLPSDINESLVVALYSK"
BASE COUNT          884 a         1488 c         1436 g         1012 t
ORIGIN      
        1 gaagtcgccc gcgatcttga ggtaatccac ggtcaggctc ttgagataac cgaaggaaga
       61 cagccccgag ccgaaatcat ccagtgcgaa acggcagccc atgtgcttga agcgcgcgat
      121 gaaatcgcgc gcacgggtga ggttggcaat ggcggcggtc tcggtgatct cgaaacagat
      181 gctgcgcgga tcaacccggt gctgcgcgaa ctgttgcatc acgaactcga gaaactgctc
      241 atccccgagc gactgaccgg agagattgat ggcgaacagt gattcgccag tcccctcgcc
      301 ctttccggtc tgcgcctggt atttttccag catgtggaac accgttcgta tcacccaacg
      361 atcgatggat ggcatcaggt gataacgctc ggcggccggg atgaaggccg cgggtagcac
      421 caggtcttcg tcctgcacgc gcaccaggat ttcatggaaa gtgccgggtt gctgcttgcc
      481 gtcccgcaac gggacgatag cctggcaata gagatcgaag ctgttgttct ccaagccctg
      541 acgcaatcgg tgcacccact gcatctcgcc gcgtcgctgg gcgaacgccc ggtcgtccgg
      601 ctggtagatg tgaatgcggt tgcgtccgcg gtccttggcg atgtagcagg cggaatcggc
      661 ggcgctcagt acttccgtca ggccgctact gtcgcgcgag atcggcacaa ggccgatgga
      721 aacgccgatc tcgaacgcgc gcttctccca catgaaccgg aaatccttga cggactggcg
      781 cagcaggtcg gcaatctgct cagccttgtc gatcgagcaa tcctcgagaa tgacgccgaa
      841 ctcatcaccc cccaggcgcg ccagcatgtc gctggcgcgg atttcctttt gcagatgact
      901 cgccagctgc ttgagcagtt catcaccggc gacatgacca caggtgtcat tcacgatctt
      961 gaactggtca aggtcgagat aaagcagcgc atgcgtgcgc tccccggcgc gcgcgctctc
     1021 cagcacgtgc tgcaggcgtg actcgaactc gcgccggttc aacaaccccg tgagcggatc
     1081 gcgcgaagcg agaaaagcca tgtgccgcgc catgctctgg atttccgtca cgtcatggaa
     1141 caccacgacc gcgccgagaa tcaccccgtc ccggtcacgg atcggcgcgg cggaatcctg
     1201 gatctcgatc ttgccgcctt catggctcat cagcaggtga tggctgtcat gacggatggt
     1261 gcggccttcc ctgagacaat aatgtatcgg gtattcctcg agacgattgg actcctcgtc
     1321 aaaaatcttg aatacctcgc tcaagggtct gccctgggct aggtcgcccc tccattcggt
     1381 gaactgttcc gcgaccggat tcatgtacac aatgcggccc gtggcatcgg tggtgatgac
     1441 cccgtcgccg atggattcga gcgtcacctg tgcgcgcacc ttttccagaa acaacgcgtc
     1501 ctccgagcgt ttcttctccc ggtcgcgcgc ctcctgcaag gccgccgcca tggcgttgat
     1561 gccgtcctcc agccgcccca cctcgccgcc cgactgcatt gacacgcggt aatcgagcac
     1621 gccgtttttg attttctcca ccgcccgggt cagcgaaatc acgggccgcg tcacgccgcg
     1681 cgccatgagc agggcgaata cgacggtcgc caccagaccg acaaggacga taaaaaggct
     1741 ctgaaacagg attttgttct gctccgtctg cagcgccgcg ctggaaagct ggaccgtcac
     1801 ccaacccgcc tgttttttcg gaacggcagc ggattcctta ttctgttttg cctcgccggt
     1861 ctcctcgctg taataaaccg gcgcgagaag cttgtagcgc gagctttccg ccaagggcgg
     1921 gggtttgttc ttgtccatcg cctcggcggc gtgcgcgagg atatcgcctt tcatgtcggt
     1981 cactgtcacc gacaggacat ttttctcctg caaggtcgcc tgcacgaagc gggcaagctt
     2041 cttgcggttg ccggacagga acgcgtcctc gctgatcgcg gccagttgcg tggcaatggc
     2101 ctgaccccgg ttgcgcagag cgtcctcgag atcctggatg cgggcactgg tgaagtgcaa
     2161 cgacaacagc agcaccgcca cggtaagcgg ggcgagggcg agaaacaaca cccgtttctg
     2221 caggttccat tctttcatgt ggttgtcatg gctgcatcag cgtgagcgga tcgccgggcc
     2281 tgaaacccag ccgcgccgcc gcgctgccgc cgttgacggc gatttcgacg agtccattgg
     2341 cattctcata ccagaacgcc gtgcccggcg gcacatccga aaaaacgcgg gcgtatttta
     2401 acacctctcc cccgacacga atggcctgcg tgttcgacac cgatgccttg cgcaggccgg
     2461 taataccgtt accgtaatga tctatataga taatttcggc caagtcgtcc ggccaaatct
     2521 catcagccgg cgccgtcagt tcgtccgacg cggcatccgg tatctcgccg cgcgctctta
     2581 acgactctgt agagcggcgt tcaggaaaat aaccagcggg ctcggagtcc ggaatttcgc
     2641 cccgtatcaa ctttgctgcg actggcgcaa ataaatcgcg tccgtgaaag ctggcggaca
     2701 gcctcgcggg acgccactga attacacgat attcatattc cgtggcgcgc cgcatgacca
     2761 tttcgaacaa gccattgtcc ggaccgacat accagcgctt gtcggcctgg agcatgaccg
     2821 ggcggcgcgc gccacccacg cctggatcga cgacacaaat gaaaaccgtg cccggcggaa
     2881 aatcgcgcgc gtaggccggc aacagatagg cgccggcgcg gatgttgaaa gcaggcacgg
     2941 aatgaaacag atcgatgacc ggcacacccg gcgcttcccg cgcgagtacc gcatgcatct
     3001 ggccgacata ggggtcggtg aggccaaact cggtaaacag gacaatcatg gcgtacctgc
     3061 cttattgtct ctcactgtgc ttttaaataa aaaagccggg catgcccggc ttttttaacc
     3121 aagggtgagc ggccgttatt ccgcggtctt ggatgtcttc ttgcccttgg cggatttctt
     3181 cctggttgtg ctcttcttgg ccttgctctt ggcggttttg ccgcccttcg ccttggcctc
     3241 tttggcaccg ccttccgtcg ttgccgcgac ttcgctcacc ggctccgtga cggccgcccc
     3301 ggcggcacgc tgttcggtca gctgcaccag cgccatgggc gcggaatcgc cacgccggta
     3361 acctgtcttg aggatgcgca ggtagccgcc cgggcggtcc ttgaagcgca cgccgagatc
     3421 gtcgaacagc ttgcccacca tatcgcggtc acgcagacgg gaataggcga ggcgccggtt
     3481 ggcgagcgag gccgttttgg ccagcgtgat gagcggctcg gccacgcgcc gcagctcttt
     3541 cgccttgggc agcgtggtca ccagttgctc atggcgcagc agcgagaccg ccatgttgcg
     3601 gaacatcgcc tcgcggtgac tgctggtgcg gttgagttta cggccgcttt tgtggtgacg
     3661 cctgattttt taaccttcga cgcttaaatc aggtggcgga gcccatctcc ggggtttttt
     3721 ccctgtcttt cagggatgcc gggggccagt tctcgagtgt catgccgagc gacaggccgt
     3781 gctgggccag gacgtccttg atctcggtga gtgacttttt gccgaggttg ggcgtcttga
     3841 gcaattcgaa ttcggaacgc tggataagat cgccgatata gaaaatattc tcagccttga
     3901 ggcaattggc tgaacgcacc gtgagctcga gatcgtccac cgggcgcagc agcagcgggt
     3961 ccatttcggg ttcgcgcttc tcttccgcct tggtctcggg actcttcagg tcgacgacaa
     4021 tggaaatctg gtcctgccgg atgttggccg cgcgccgtac cgcctcttcc gggttgatgg
     4081 cgccgttggt ttcgatgtcg agcaccagct tgtcgaggtc ggtgcgctgt tccacgcgcg
     4141 cattctcaac cgtataggcc acgcgacgga tcgggctgta ggaggcatcg agctgcatga
     4201 cgccgatggt gcggccctcg gtgccggggg cgcgcgccgt aaccggctgg tagccgcgtc
     4261 cgcggttcac cttgagttcc atgttgagcg tcccgtcctt ggtcagggtc gcgatcacgt
     4321 gcttcggatc caccacttcc acgtcatgct ccagctggat gtcgccggcc gttactacgc
     4381 cgtgtccttt cttggcgagc ttgagcgtgt cttagtcgcg gccatgcatg cgcagcgcga
     4441 ccgtcttgag attgagcaga atctcgatca cgtcctcctg cacaccctcc atggtggagt
     4501 attcgtgcag cacgccgtcg atcttcacct cggtgatcgc actgcccggc atcgaggaca
     4561 gcaggatgcg gcgcagcgcg ttgccgagcg tgtagccgaa accgcgttcg agcggctcca
     4621 gcgtgatttt cgcgtgcgtg gaagaaagcg tctgcacgtc caccagacgc ggcttcagaa
     4681 attcgcttgc agaactctgc atgagggcgt cctcgttaga tactgattac gttacttcga
     4741 ataaagcgct acgacgagac tttcgttgat atccgacggc aagtcgttgc gttccggcac
     4801 gctcttgaac gtgcccttca
//
LOCUS       DBNGZE010000365         3017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_467674, whole genome shotgun sequence.
ACCESSION   DBNGZE010000365 DBNGZE010000000
VERSION     DBNGZE010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_467674"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC16FV_09385"
     CDS             complement(<1..462)
                     /locus_tag="AC16FV_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR2850302.1"
                     /translation="MEFIVKSGTPEKQRTGCIVVGVFEARKLSAVAHQIDEVAGGAIG
                     AILRRGDIEGKPGQTLLLHNIPNLPSERVLLVGCGKEKEFNESRYRDATAKTIGVLKD
                     TGTTEITSYLTEIDVKGRDIAWKVRQAVEVTEAALYRFDQLKSKPNNQRRAL"
     gene            678..1775
                     /gene="lptF"
                     /locus_tag="AC16FV_09390"
     CDS             678..1775
                     /gene="lptF"
                     /locus_tag="AC16FV_09390"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR2850303.1"
                     /translation="MIINRAFYREVAVTTLGIAVVLLIVMTMMNLTLLLGRAVRGGTP
                     ESVLYILLGYQMLGKIDVLLPLAFYLGVLLTLSRWYRDSEMTVLAACGVGLTHFLRPV
                     MLIGLGMGVVVMATSFYTTPLTVRQIEKVKAESAHRTEPDQVAPGVFTESPGTGRIFY
                     AEKIRGNGQLEGVFVSSLEEGGQGVLVAKSGHPFTDPKTGDKFVALRDGTLYEGEPGA
                     ANYRILEFGVYNLRIEPKMVDEPTVPMSGLPNMALLGRLGNPQANAELHWRLGKTVVL
                     FVLALFAMAFAYTDMRRGRMSNLFVAIVIYFVYSNLLGIGETMLQNGRVPAALGLWWV
                     HGGIVLAAAYLLWQRSRNRPLLALSLRPRRA"
     gene            1772..2854
                     /gene="lptG"
                     /locus_tag="AC16FV_09395"
     CDS             1772..2854
                     /gene="lptG"
                     /locus_tag="AC16FV_09395"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR2850304.1"
                     /translation="MIMPSLLDRLIARHVLYSTLLVLNVLVALAVFFVLIDALKDYGE
                     ANFGMFELIKYVVLRQPQQVYELSPVGALIGTIMGLSTLALNSELTAMRAAGVSVARI
                     VLATMKVGLLFAAVILLVGEYVVPASENLAQTGRARALETPLQKKSTGIWLRSGTTFV
                     NIGEVLPDLSLLRMNIYDFDHNSRLRIQTSAQRAHHDGGVWRLQDVRESLIVNNEAVQ
                     QRRAPEQDWHSALSPEVVSLFAIQPDSLSIQQLHYYIGHLEQNNQDTRTFRLAFWQKA
                     LMPLATLIMVLLATPFVFRQLRSSGLSHRVFIGIMLGLTFIIFHKSLGYFGALYALPP
                     AVASLLPIAVFFVVALFLLRRAARGL"
BASE COUNT          503 a          923 c          913 g          678 t
ORIGIN      
        1 caaggcacga cgctggttgt tgggtttgct cttgagctgg tcgaagcggt acagcgcggc
       61 ttccgtcact tccaccgcct gacgcacttt ccaggcgatg tcgcggccct tgacgtcgat
      121 ctcggtgagg taggaggtga tttcggtggt gccggtgtcc ttgaggacgc caatcgtttt
      181 ggcggtggcg tcgcggtaac ggctttcgtt gaattccttt tccttgccgc aacccacgag
      241 cagcacgcgt tcgctgggaa ggttcgggat gttatgcagc agcagggtct ggccgggttt
      301 cccctcgatg tcgccgcgcc gcagtatcgc gccaatggcg ccgcccgcga cttcatcgat
      361 ctggtgcgcc acggcggaca gcttgcgcgc ctcgaaaacg cccaccacga tgcagccggt
      421 acgctgtttt tcgggtgtac cgcttttaac gatgaattcc atgccgattc cccgtggtta
      481 tttcacgcct caacgctgcg cgatatggta tatttttgtc gacttcgggt cacggttccg
      541 ttttgcaatc aagccattag ggatgactgt gcgtgaaaaa acggtatttt ggatgctttc
      601 ccctctggcg cgcccattga gcggcatttt ccaatgtttg cgcgtccctt gtcaaaagcc
      661 aacccggcgc tgacgccttg atcattaatc gcgcgtttta ccgggaagtt gccgtgacca
      721 ccctgggcat tgcggtggtg ctgctgatcg tcatgaccat gatgaatctg acgctgctcc
      781 tgggacgggc ggtgcgcggc ggaactccgg aaagcgtgct gtacatcctg ctcggttacc
      841 agatgctcgg caagatcgac gtgctgctgc cgctcgcgtt ttaccttggc gttctgctga
      901 cgctgtcgcg ctggtaccgc gacagtgaga tgacggtact ggccgcctgc ggggtggggc
      961 tgacacactt cctgcgcccg gtgatgctga tcggcctcgg catgggggta gtggtcatgg
     1021 cgacctcgtt ttacaccacg ccgcttacgg tgcgccagat cgaaaaggtc aaggccgaaa
     1081 gcgcgcaccg caccgagccc gaccaggtgg ctcccggtgt ctttaccgaa tcgcccggca
     1141 ccggccggat tttctacgcc gaaaaaatcc gcgggaacgg ccaactggaa ggagtttttg
     1201 tcagcagcct ggaagagggc ggacagggcg tgctggtcgc gaaatccggc catccgttca
     1261 ccgatcccaa aaccggcgac aagttcgtcg cgctgcggga tggcacgctt tacgagggcg
     1321 aacccggcgc cgccaattac cgcatcctcg aattcggcgt ctacaacctg cgcatcgaac
     1381 ccaagatggt ggacgaaccc acagtgccga tgtcgggtct gccgaacatg gcactgctgg
     1441 gacggctcgg caacccacag gccaacgccg aactgcactg gcgcctgggc aagaccgtcg
     1501 tgctgttcgt gctggcgctg ttcgcgatgg cgttcgccta caccgacatg cgccgtgggc
     1561 gcatgtccaa tctttttgtc gccatcgtga tttatttcgt gtattccaac ctgctcggca
     1621 tcggcgaaac catgctgcag aacgggcgcg tgcccgcggc attgggttta tggtgggtgc
     1681 acggcggtat tgtcctggcg gccgcgtatc tgttgtggca gcggagccgc aaccgtccgt
     1741 tgttagccct ttccctgagg ccgagacgcg catgatcatg ccctcgctgc tcgaccgcct
     1801 gatcgcacgg catgtgctct acagcaccct gctggtgctc aacgtgctgg tggcgctggc
     1861 ggtgttcttc gtcctgatcg acgccctcaa ggattacggc gaggctaatt tcgggatgtt
     1921 cgagctgatc aagtacgtcg tgttgaggca gccgcaacag gtctacgaac tgtcgccggt
     1981 cggcgccctg atcggaacca tcatgggact gtccaccctg gcgctcaatt ccgagctgac
     2041 cgccatgcgc gccgccggtg tgtcggtggc gcgcatcgtg ctggcgacga tgaaggtggg
     2101 gctgttgttt gcggccgtga tcctgctggt gggcgaatat gtcgtgccgg cctcggaaaa
     2161 cctggcgcag accggccgcg cgcgggcgct ggagacgccg ttgcagaaaa aaagcaccgg
     2221 catctggctg cgcagtggca cgacgttcgt gaacatcggc gaggtgctgc cggatctcag
     2281 cctgttgcgc atgaacatct atgacttcga ccacaattcc cgcctgcgca tccagacttc
     2341 cgcccagcgc gcgcaccacg acggcggcgt atggcgcctg caagacgtgc gcgaaagcct
     2401 gatcgtcaac aacgaggcgg tccagcaacg ccgcgcgccg gagcaggact ggcactcagc
     2461 gctctcgccc gaggtggtgt cgctgttcgc gatccagccc gacagtcttt cgatccagca
     2521 attgcattat tacatcggcc atctggagca gaacaaccag gacacgcgca ccttccggct
     2581 cgcgttctgg cagaaggctc tcatgccgct ggccacgctc atcatggtgc tgctggccac
     2641 accttttgtg tttcgccagc tccgcagcag cggcctgagc catcgcgtat tcatcggcat
     2701 catgctgggg ctgacgttca tcatcttcca caaaagcctc ggctatttcg gcgcacttta
     2761 cgcgttgccg cccgcggtcg cttcgctact gcctatcgcc gtcttttttg ttgttgcgct
     2821 gttcctgctg cgccgcgcgg cgcgcgggtt gtgatcctta tctgggtgtc cgtggaaacg
     2881 ggtacatctc actccgtccc cttttactcc gggggaggcc tagtctgacc ctattgcttc
     2941 tcgtttctta gttttttttc gccaactcta agaatctggc gcatatcgga gtatcgtcac
     3001 tatctagcca aacgctt
//
LOCUS       DBNGZE010000366         1688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_954506, whole genome shotgun sequence.
ACCESSION   DBNGZE010000366 DBNGZE010000000
VERSION     DBNGZE010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_954506"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="AC16FV_09400"
     CDS             complement(<1..594)
                     /locus_tag="AC16FV_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2850305.1"
                     /translation="MELATDARPLDRRVVITALGIAQILAWGTSFYFPAVFAEPIVAE
                     TGWSLGFVVGGTSIGLLTAGLISPQVGRIIDAHGGRPVLLASSAFYAAGLALAGFAHA
                     LPFYLLAWVLIGIGMGTGLYDAVFAALGRMYGSKARGPITNLTLFGGFASTICWPLSA
                     FMIDHVGWRSACFIYAALHLFVMLPLQVIVVRTPEKIK"
     gene            complement(607..1371)
                     /gene="modA"
                     /locus_tag="AC16FV_09405"
     CDS             complement(607..1371)
                     /gene="modA"
                     /locus_tag="AC16FV_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR2850306.1"
                     /translation="MNSHIWRVLVITATVSLSGMASAAEVKVLTAGAFKQVVLALVPN
                     FEKQTGNKVVVDNDTAGGLKKRIEGGAAFDVAVITPAVIDDLVAAGKIVSGSRVNLAT
                     VGVGIVVKEGAPKPDVSTVEAFKSTLLAAKSVAYIDPASGGSSGVYIDKLLERLGIAD
                     QVRPKAKLKKGGYVAELVVSGEAELGLHQISEIVPVKGAVLVGPLPKEIQNTTTYAAG
                     LSASSQNKDAAQALIKMFSGPAAATVFKFKGMEPAN"
BASE COUNT          376 a          508 c          459 g          345 t
ORIGIN      
        1 tttaattttt tctggcgtgc gcaccacgat cacttgcaac ggcaacatca cgaacaggtg
       61 cagtgctgca tagatgaaac atgcgctacg ccaccccaca tgatcgatca tgaaagcact
      121 gagcggccaa cagatggtgc tggcgaagcc gccgaacagc gtgagattgg tgatcggtcc
      181 gcgcgcttta ctgccgtaca tacggccgag cgcggcaaat actgcatcat agaggcctgt
      241 gcccatgccg ataccgatca gcacccaggc cagaagataa aacggcagcg cgtgcgcgaa
      301 acccgcgaga gctaggcccg cggcatagaa tgcggagctc gccagtaaca ccggtcgtcc
      361 gccgtgcgca tcgataatgc ggccgacctg cggcgagatc aggccagctg tgagtaggcc
      421 gatcgaggtg ccaccaacga cgaaaccgag cgaccagccc gtttcagcga caatcggttc
      481 ggcaaagacc gccggaaaat aaaacgaagt cccccaagcc aggatctggg caatgcccaa
      541 cgcggtaatg acaacgcggc ggtcgagcgg tcgcgcatct gttgccaatt ccaaaatgcg
      601 tggtccttag ttcgccggct ccatgccttt gaatttaaac actgtggcag cggcgggtcc
      661 ggagaacatc ttgatcaacg cctgtgccgc gtccttgttt tggctggaag cgctcaagcc
      721 ggcagcataa gtcgtggtgt tctggatttc tttcggaagc ggcccaacga gcacggcgcc
      781 tttcactggc acaatttcgc tgatctggtg caggccgagt tcggcctcac cgctgacaac
      841 gagttcagcg acgtagccgc ctttcttgag cttcgccttg ggccggacct ggtcggcgat
      901 gccaagccgc tccagcaatt tgtcgatata gaccccgctc gagccgccgc tcgcgggatc
      961 gatataggca accgatttgg ccgccaacaa cgtgctctta aaggcctcca ccgtgctcac
     1021 gtccggcttg ggtgcgcctt ctttcacgac gatgccaaca ccaacggtgg caagattgac
     1081 acggctgccg gaaacaattt tacccgcggc cacgagatcg tcgatgacgg caggcgtgat
     1141 tacagcaacg tcgaatgccg ctccgccctc gatgcgcttc ttcaggccgc cggcagtgtc
     1201 gttgtcgacg acgaccttat tgccagtttg cttttcgaaa ttcggcacca gcgccaacac
     1261 tacctgtttg aaagctcccg cggtgagcac cttgacctcg gcggcggagg ccatgcccga
     1321 aaggcttacc gtggcagtga tcacgagcac gcgccagata tgcgaattca tgggtctttc
     1381 ctcccggttc taaggagaaa atctacacga tcgcagccct aacgggggct gaaattggcc
     1441 attttcgcag cagcgaaaaa cctcccgtca aaagcgtagc gaatgtgccc gaaattccgt
     1501 tttgatccta attttcggtt agtttagcta tgcgtttttc gcttagctgc gacgcaacat
     1561 tattgctttt catccgtctc aacatcctta ttttcctccc gaacgaccgg ccttggccgg
     1621 accaaaggaa agaaggacca caatgtttgc cgcactcgtc cgcttcatcc aggaatggaa
     1681 gcgctaca
//
LOCUS       DBNGZE010000367         4733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_190403, whole genome shotgun sequence.
ACCESSION   DBNGZE010000367 DBNGZE010000000
VERSION     DBNGZE010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_190403"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1891)
                     /locus_tag="AC16FV_09410"
     CDS             complement(119..1891)
                     /locus_tag="AC16FV_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIR2850307.1"
                     /translation="MSFAQEAVVFLAAAVIAVPLFKKLGMGAVLGYLFAGVLIGPWLL
                     GFISDVDNILHFAELGVVLLLFVIGLELRPSRLWELRRPIFGLGGAQFIVTGVVLGAA
                     GMALGLPATTAFTVGLVLSLSSTAFALQILAERKKLTTHYGRASFTILLLQDLAAIPL
                     LALIPLLNGSHLDQGWDLLSALKPVAIVVAVVAGGRWLLRPFLRLAASATSNEVFIAA
                     ALLVVIGTALLVQWAGLSMALGAFLAGVLLADSEYRHELEADIEPFKGLLLGLFFIAV
                     GMAMNLGLVVERPLAVLGLVAGLVAVKSLLLYGLGRLAKLGHGAAFNLALYISQGGEF
                     AFVLFGAAVGAQVLDKTLAELLIVVVSVSMMVTPLLVTLNEKLFKIGCVTEQAREFDK
                     IEAREHRVIIAGFGRFGQMIARTLRMKKIPFTVLEASFEQVDFVRKFGTKVYFGDASR
                     LDLLRAARAESADVFVLAIDDIEASIKTAQMVKRHYPHLKIYARARNRVHAYRLMDIG
                     VDKQIRETLLSSIELARDVLMALGHTETEANEAVRRFRQHDEALLERQHKIHHDEAQL
                     IASVRQGAEELERLFEEDTSGTAK"
     gene            2104..2550
                     /locus_tag="AC16FV_09415"
     CDS             2104..2550
                     /locus_tag="AC16FV_09415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR2850308.1"
                     /translation="MTPSAKRFILDQLKTNRIMSLATMRSDGYPQATTVTYANDGLTL
                     YFCCYNKSQKVRNLRRSPKVSLTINKDTADWKKITGLSMGATAKVLERPADTQRALAL
                     LSRKFKDMKNLSEEDLAETAFVRVQPKVVSMLDYRREFGWTKLVRV"
     gene            2709..3116
                     /locus_tag="AC16FV_09420"
     CDS             2709..3116
                     /locus_tag="AC16FV_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850309.1"
                     /translation="MSKTIQQKMHEQHRRWQSDSETWRSDIDEWNKELRVALGDLREV
                     EDMLRDALDALAYHADSVWESEQRRCAHELELREEAMSGAEKKTDKTWAKLHREHAAR
                     HERMADAHARIKKYQHKVVAEVMRLLRTARKGM"
     gene            3549..4715
                     /locus_tag="AC16FV_09425"
     CDS             3549..4715
                     /locus_tag="AC16FV_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850310.1"
                     /translation="MNYLRRIAAIAVAALSFISFDASAVIDANAPVVKLRYDNPSIPD
                     DGCTEGGVAINNCFTGIGALNTWIRDTRMPTTAKPLLVEIGPGSYAGKFNCTDLSGIT
                     LRGSGRDNTVFKGGMYLENCDMLSVSSLQINGSYYIIEWSGTGRSTWTDVEVIGSGIG
                     WYDDSPTCTRPGTAHYWYASRFEVHPYLTQAATYVANCGEHWFQGSELTATHAALPGV
                     PYPSAGTGNVIRASNSEVHVYGSVIRMLAPSVYQGTITSTVAVWAMDSGAEIHIHGTG
                     IDVLSDVAIPVTVLQSTGGMIHADVSAYNLKSAAGGSVTRIYNQGGHVHAPYQWQHIP
                     DPAVIPSYASTNGADTTNVTAGTSDGHPHVSVYSSTCPSNARWYDQVDKVCRSQ"
BASE COUNT         1041 a         1378 c         1416 g          898 t
ORIGIN      
        1 ctataagtat cgctcagaat taaagaggct agacacgatt tcttgtgctg atttggccct
       61 ctcgatcctg atttgcacca aagttcccgc caccgtttcc ggataagcga ccccattatc
      121 acttagctgt tcccgaggta tcttcctcga acaatcgttc cagttcctcc gcaccttgtc
      181 gcacagaggc tatcagttgc gcttcgtcgt gatgaatttt gtgttggcgt tcgagcaagg
      241 cctcatcgtg ctggcggaac cggcgcacgg cttcgttcgc ttcggtttcc gtgtgtccca
      301 acgccatcag cacgtcacgc gccagttcaa tgctcgatag cagcgtttca cggatttgtt
      361 tgtccacgcc gatatccatg agccggtagg catggacgcg gttgcgggcg cgggcgtaga
      421 tcttgaggtg cgggtaatgt cttttgacca tctgcgcggt tttgatcgag gcctcgatgt
      481 cgtcgatggc cagcacgaaa acatccgccg attcggcgcg cgcggcgcgc agcaggtcga
      541 ggcgcgaggc gtcgccgaag tacaccttgg tgccgaattt gcgcacgaag tcgacctgct
      601 cgaagctcgc ttccagcacg gtgaagggga tttttttcat gcgcagcgtg cgcgcgatca
      661 tctgaccgaa gcgcccgaac ccggcgatga tgacgcggtg ttcgcgcgcc tcgattttgt
      721 cgaattcccg ggcctgctcc gtgacgcacc cgatcttgaa cagcttttca ttcagcgtca
      781 cgagcagggg ggtgaccatc atggaaaccg acaccaccac gatcagcagt tcggccaggg
      841 ttttgtccag cacctgcgcg cccacggccg cgccgaacag gacaaaggcg aattcaccgc
      901 cctgggaaat atacagggcc aagttgaatg ccgcgccgtg acccagtttg gccaggcgcc
      961 ccagcccgta gagcagcagc gatttaaccg ccaccaatcc cgcgaccaac ccgagcacgg
     1021 cgagtggccg ctcgaccacc agcccgaggt tcatggccat gccgaccgcg atgaaaaaca
     1081 gcccgagcag caggcccttg aacggttcga tgtcggcctc cagttcgtgg cggtattccg
     1141 aatcggccag caacacgccc gccagaaacg cccccagcgc catggacaga ccggcccatt
     1201 gcaccaacaa cgccgtgcct atcaccacca gcaaggccgc ggcgatgaat acctcgttac
     1261 tcgtggcgga ggcagccaga cgcaggaacg ggcgcagcag ccagcggccg ccggcgacga
     1321 cggccacgac gatggccacg ggcttgaggg cggacagtaa atcccagcct tggtcgaggt
     1381 ggcttccgtt caataacgga atcagcgcga gcagggggat ggcggccaga tcctgcaaca
     1441 gcagtatcgt gaacgacgcg cgtccgtaat gggtggtgag tttttttcgt tccgccagga
     1501 tttgcagcgc gaacgcggtg gaggaaagcg acagcactag gccgaccgtg aacgcggtcg
     1561 ttgccggcag ccccagcgcc atgccggcgg cacccaggac cacgccggta acgatgaatt
     1621 gcgcgccgcc gagaccgaag atgggacggc gcaattccca caggcgcgac ggccgcagtt
     1681 ccagcccgat cacgaacagc agcaagacca cgccgagctc ggcgaaatgc agaatattgt
     1741 ccacgtcgct gatgaagccg agcagccacg gaccgatgag cacgccggcg aacagataac
     1801 ccagcaccgc gcccatgccg agttttttga acagcggtac cgcgatgacc gccgccgcga
     1861 gaaacacgac cgcttcttgc gcgaatgaca tgctcattga aggctgcctt gggattcagc
     1921 gtgcaggaac aatcatactc ctgaccgccg tatgaccgac aagccagcgc cgcggcgcgg
     1981 gagattaaag cgaccgggca cggttttcgg ttgatcctga tttgcgtcaa agccgcctcc
     2041 caaagcttgt gccatgctcc acagctcaag tcagtgcatg ccctgcccgg gaaggaggga
     2101 accatgaccc cgtcagccaa gcgcttcatt ctcgatcagc tcaaaaccaa ccgcatcatg
     2161 agcctcgcca ccatgcggtc cgacggctac ccgcaggcga cgacggtgac ctacgccaac
     2221 gacggcctga cgttgtactt ctgctgctac aacaagagcc agaaggtccg caacctgcgg
     2281 cgctcgccca aggtctcact cacgatcaac aaggacacgg ccgactggaa gaagatcacg
     2341 gggctgtcca tgggcgccac cgcgaaggtc ctcgagcgcc cggccgacac ccagcgcgcg
     2401 ctcgcgctgc tctcgcgcaa gttcaaggac atgaaaaacc tcagtgagga ggatctggcg
     2461 gaaacggcgt tcgtgcgggt gcagccgaag gtcgtctcga tgctcgacta ccgccgcgaa
     2521 tttggctgga cgaaattagt gcgggtgtag cgggttgctg aaaaagtccg ttcgtgcttc
     2581 gacaagctca gcacgaacgg acttcgacga tggcaaatcg aaggcttggc cgttcgccct
     2641 gagcccgtcg aagggtgaac ggccacgaga gaggcgtttt tccggtcgtt tacggcaagg
     2701 agagtccgat gagcaagacc atccagcaaa agatgcatga acagcaccgc cggtggcagt
     2761 ccgacagtga aacctggcgg tctgacatcg atgagtggaa caaggaactg cgcgtagcgc
     2821 tcggcgacct gcgcgaggtc gaggacatgc tgcgcgacgc gctcgacgcc ctggcctacc
     2881 atgcggacag cgtgtgggaa agcgaacagc gtcgctgcgc tcatgagttg gaactgcggg
     2941 aggaggccat gagcggggcc gagaagaaaa cggacaagac ttgggccaaa ctccatcggg
     3001 agcacgccgc gcggcacgag cgcatggcgg acgcccacgc ccgcatcaag aagtatcagc
     3061 ataaagtcgt ggccgaggtg atgcgcctcc tgcgcacggc ccgcaagggc atgtaaaaag
     3121 aaacggcccc tcgcggggcc ttttaggagg ataaacgtct gttccagacg agcgggcgtt
     3181 tctttatcgg attcggtgcg cttcagtatg gcaaggaggc caaggcggtg gattactgaa
     3241 gcggggagcg gggatttaag aaatcggaag gaatatcagc tcttgctaaa aaacccgagc
     3301 acttccgtgc tcgggttttt gttttggcga aggtgatttt ttcaaagaag aatccggttc
     3361 gttcgttgac acatggagag tgaccacaga tactcgcccg gttccggttc tgccattttc
     3421 agaagcgctg caagttcgac agcgcgcccc ggattaccgt aacagcggga gaaggagagg
     3481 acgagggaag aaggtgacag gttcgggacg aaccctttca actaccatgg aacaggagga
     3541 aaagaaaaat gaattacctt cggagaattg ccgcgattgc cgttgcggca ttgagtttca
     3601 tttcgttcga tgcatctgcg gtaattgatg cgaatgcgcc agtcgtcaaa ctccgctacg
     3661 ataatccgtc gatacccgac gatggatgca cggagggcgg cgttgccatt aacaactgtt
     3721 ttacgggcat tggggctctc aacacatgga tccgtgacac cagaatgccc accacagcaa
     3781 agccgcttct tgtcgagatt ggccccggtt cctacgcggg taaatttaac tgtaccgatc
     3841 tcagcgggat tacgctgcga ggctcgggcc gtgacaacac ggtattcaag ggtggcatgt
     3901 acctcgagaa ctgcgacatg ctcagtgttt ccagcctgca aattaacggc tcgtactaca
     3961 ttatcgaatg gtcggggacc ggcagatcaa catggacgga cgttgaggta attggatccg
     4021 gaattggctg gtacgatgat tcgccgacct gtacgaggcc ggggactgcg cattattggt
     4081 acgccagtcg tttcgaggtg catccttatt tgacgcaggc agctacatac gtcgcgaact
     4141 gcggagagca ttggttccag gggtcggaac ttaccgcaac tcacgccgcc ctgccggggg
     4201 ttccgtaccc gtcggctggc acgggtaatg tgatacgtgc ttcgaatagc gaagttcatg
     4261 tctacggttc ggtcattcgc atgttggcgc catccgtata tcagggaact attaccagca
     4321 ctgtagccgt gtgggccatg gactcaggag ccgaaatcca tatccacggc actggaattg
     4381 acgtgctgtc cgatgttgcc attccagtga ctgtgttgca gtcgactggc ggcatgattc
     4441 atgccgatgt gtcagcgtac aatctgaaat ccgctgccgg cggttcggtt acgcgcatat
     4501 ataaccaggg cggccatgtg catgcgccgt atcagtggca gcatattcct gatcccgcgg
     4561 tcatccccag ctatgcgtca accaatggcg ctgacaccac gaacgtaacc gctggtacca
     4621 gcgatggtca tccgcatgtg tcggtttaca gttcgacctg cccgtccaat gcgcgttggt
     4681 atgatcaggt ggataaagtc tgccgcagcc agtaactggc gctccctgac ggc
//
LOCUS       DBNGZE010000368         5691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_675611, whole genome shotgun sequence.
ACCESSION   DBNGZE010000368 DBNGZE010000000
VERSION     DBNGZE010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_675611"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16FV_09430"
     CDS             complement(<1..196)
                     /locus_tag="AC16FV_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR2850311.1"
                     /translation="MNNNYYDVLSRKSWRAHPRFAQAGYSIFDLIITSAVAGVLSLGA
                     VGMNGLVQDARMTTVVNQIMG"
     gene            complement(341..1528)
                     /locus_tag="AC16FV_09435"
     CDS             complement(341..1528)
                     /locus_tag="AC16FV_09435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2850312.1"
                     /translation="MRLSGRVNRIKPSPTLAVDARAKALKAQGKDVISLGAGEPDFDT
                     PDHIKEAAIRAIREGFTKYTAVDGTPGLKQAVIGKFKRDNSLDYVPDEILVSVGDKQS
                     FFNLAQALLDNGDEVIIPAPYWVSYPDMVLLADATPIIISAGIDQAFKIRPEQLVRSI
                     TRKTRLLVINSPSNPTGAVYTRAELAALGEVLRKHPHVLIATDDMYEHILWAKEPFSN
                     ILNACPDLKDRTMVLNGVSKVYAMTGWRIGYAAGPKSLITAMRKIQSQSTSNPTSIAQ
                     VAAEAALNGDQGCVTPMVRAFKERHDRVVSRLNKIRGVRCLPSEGTFYAFPDFSQAIA
                     ATNDVNNDAEMADYLLNKAEVALVPGSAFGAEGHLRLSYATSQATLDKALDRIESLLG
                     TVT"
     gene            1609..3684
                     /gene="uvrB"
                     /locus_tag="AC16FV_09440"
     CDS             1609..3684
                     /gene="uvrB"
                     /locus_tag="AC16FV_09440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR2850313.1"
                     /translation="MSHRFELASSFSPAGDQPQAVTALIEGLSQGEAYQTLLGVTGSG
                     KTFTIANVIQQVQRPTLVLAPNKTLAAQLYSEFRDFFPRNAVEYFVSYYDYYQPEAYV
                     PSSDTYIEKDASINEQIEQMRLSATKALLEREDSIIVATVSAIYGLGDVDAYHGMILH
                     LRQGTAMPQRAILQRLTEMQYTRNDIELHRGTFRVHGDVIDIFPAESEKLAVRVELFG
                     DDIEALSEFDPLTGEVDNRPVRFTIYPSSHYVTPRETIMSAINEIKAELRERLEQLRS
                     VDKLVEAQRLEQRTLYDLEMMREVGYCSGIENYSRYLSGRKAGEPPPTLIDYLPKNAL
                     LVVDESHVTIPQLGGMYKGDRSRKETLVEYGFRLPSALDNRPLRFDEFERLMPQAIFV
                     SATPGPYELQRSQGVVVDQVVRPTGLIDPEVEVRPATAQVDDLLSEVRARVAAGERVL
                     VTTLTKRMAEDLTEYLHEHDVRVRYLHSDIETIERAEIIRDLRAGTFDVLVGINLLRE
                     GLDIPEVSLVAILDADKEGFLRSTRSLIQTIGRAARHIHGKAILYADRMTDSMRAAID
                     ETERRRLKQIAYNEEHGITPKGIIKAVKEIIEGVTPAHPLYDTKDYGTKSAHAGHEFL
                     HAAEEEAEYAAMAPSALAKLLDKLEKQMYAHARNLEFEKAAAVRDRIHAIRSRKFGVI
                     EGRGQKRGR"
     gene            3751..3825
                     /locus_tag="AC16FV_09445"
     tRNA            3751..3825
                     /locus_tag="AC16FV_09445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3783..3785,aa:Val,seq:gac)
     gene            3926..>5691
                     /gene="thrS"
                     /locus_tag="AC16FV_09450"
     CDS             3926..>5691
                     /gene="thrS"
                     /locus_tag="AC16FV_09450"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR2850314.1"
                     /translation="MPVITLPDGSRREYPKPVTVAEIAASIGAGLARAALAGRVNDKL
                     VDTSFLVDQDATVAIVTDKSPEGIEVIRHSTAHLLAQAVKELFPEAQVTIGPVIENGF
                     YYDFAFRRGFTEDDLAKIEQRMHEIAKRDYPVARTVMPRDEAIKFFRDMGEEYKAQII
                     ADIPTGEQISLYRQGEFIDLCRGPHVPSTDKLKAFKLMKVAGAYWRGDSRNEMLARIY
                     GTAWTSKEALDAYLHQIEEAEKRDHRKIGRQLDLFHMQEEAPGMIFWHERGWRLYQTI
                     VQYMSQLWREHGYREIRTPQLVDRSLWEKSGHWEKFHSDMFITESEKRLYAVKPMNCP
                     CHIQVYNQGLKSYRDLPLRLAEFGSCHRNEQSGALHGLLRVRNFVQDDAHIFCTENQI
                     QDEVSAFIDLLHRVYADFGFREVIYKLSTRPPNRIGSDESWDKAEHALEQALNAKGLK
                     WDLQPGEGAFYGPKIEFSLKDSLGRVWQCGTIQVDFSMPGRLGATYIAEDGSKQVPVM
                     LHRAILGSLERFIGILIEEHAGALPAWLAPVQAVVLTITDEQQDYARKLEKTLAKHGW
                     RVESDLRNEKIGFKIREHTLQR"
BASE COUNT         1185 a         1651 c         1721 g         1134 t
ORIGIN      
        1 ctcccatgat ctgatttaca acggtcgtca tgcgtgcgtc ctgaaccagg ccgttcatgc
       61 cgaccgcgcc aaggctgagc acgccggcca cagcggaggt gataatcagg tcgaatatgg
      121 aataacccgc ctgcgcaaaa cgaggatggg cgcgccatga tttgcgcgac aagacatcat
      181 aataattatt gttcatctac ccactcctga ttgttgtttt tgttatgacc accggcctga
      241 gatggcaggt ggttttttac tgaggcccgt tgaggacttg ttttgggcga gattatgcct
      301 gttcaggcta tgcctgaaaa gccggcacag gggtatgagg ctaagtgacg gtaccgagaa
      361 gactttctat ccggtccagc gccttatcca gcgttgcctg ggaggtggcg tacgacaggc
      421 gcaggtggcc ttcggcgccg aaggccgagc cggggaccag cgccacttca gctttattca
      481 gaaggtaatc ggccatttcg gcgtcgttgt tcacgtcgtt cgtggcggca atcgcctgac
      541 tgaaatccgg aaacgcataa aaagttccct cggaaggcag gcaacgcacg ccgcggattt
      601 tattcaggcg gcttaccacg cgatcatgcc gctccttgaa ggcgcgcacc atcggcgtga
      661 cgcagccctg atcgccattc aacgccgctt cggcggcgac ctgtgcgatg gaggtcggat
      721 tggaagtgct ctgtgactga atcttgcgca tggccgtgat gagagacttc ggtccggcgg
      781 cataaccgat gcgccagccg gtcatggcat aaacctttga gacgccgttc agtaccatag
      841 tgcgatcctt cagatcgggg caggcgttga gaatgttgct gaagggttcc ttcgcccaca
      901 ggatatgttc atacatgtcg tccgtggcaa tcagcacgtg cggatgcttg cgcaacacct
      961 cgccgagcgc ggcgagttcc gcgcgtgtat agacggcgcc agtggggttc gatgggctgt
     1021 tgatgacgag tagccgcgtc ttgcgggtga tggagcgaac gagttgttcg gggcggatct
     1081 tgaacgcctg atcgatacca gcggaaataa tgatcggcgt ggcatccgcc agcagcacca
     1141 tgtcgggata ggacacccag tatggcgccg gaatgatgac ttcatcgccg ttgtcgagca
     1201 gcgcctgcgc cagattgaag aagctctgct tgtcgcccac cgagaccagg atctcatcag
     1261 gcacatagtc gaggctgttg tcgcgcttga acttgccgat aaccgcctgc ttgagtcccg
     1321 gcgtgccatc cacggccgtg tatttggtaa aaccctcacg aatggcgcgg atggcagctt
     1381 ctttaatatg atccggcgtg tcgaaatcgg gttcgcccgc gcccagactg atgacgtcct
     1441 tgccctgcgc tttgagcgcc ttggcgcggg catcgacagc aagagtaggg gagggcttga
     1501 tgcggttgac gcggcctgac aggcggatgg tggacaacgg ttcctcgcga attcaagttt
     1561 tgaaaaacgc ggtaatatac ccgcaagcca gcggaaaaac gatagcgtat gagccaccga
     1621 ttcgaactag ccagttcttt ttcgcccgcc ggcgaccagc cgcaggcggt cacggcgttg
     1681 atcgaggggc tttcgcaagg cgaggcctat cagacccttc tgggtgtgac cggctccggc
     1741 aagaccttta ccatcgccaa tgtcattcag caggtgcaaa gaccgacgct ggtgctggcg
     1801 cccaacaaga cgctcgccgc gcagctgtat tccgagttcc gcgacttctt cccgcgcaat
     1861 gccgtggaat attttgtgtc gtattacgac tactaccagc cggaggccta tgttccctcc
     1921 tcggatactt atatagagaa ggacgcgtcg atcaacgagc aaatcgagca gatgcgcctg
     1981 tccgccacca aagcgttgct cgaacgcgag gacagcatca tcgtggccac ggtgtccgcc
     2041 atctacggtc tgggcgacgt ggacgcctat cacggcatga tcctgcatct gcgtcagggc
     2101 acggccatgc cccagcgcgc tattctgcag cgtctcaccg agatgcaata cacgcgcaac
     2161 gacattgaac tccaccgcgg aacctttcgg gtgcacggtg atgtcatcga tatcttcccc
     2221 gccgagtcgg aaaagttggc ggtgcgggtt gagctgtttg gcgatgatat cgaggcgctt
     2281 tccgagttcg atccactgac cggtgaggtc gataaccgcc cggtgcggtt cacaatttac
     2341 ccctcatctc attacgtcac gccgcgcgag accatcatga gcgcgatcaa tgagatcaaa
     2401 gccgagctgc gtgaacgtct ggagcaattg cgcagcgtgg acaaactggt cgaagctcag
     2461 cggctggagc agcgcacgct ttatgatctg gagatgatgc gcgaggtggg ttactgctcc
     2521 ggcattgaaa actactcgcg ctatctttcc ggacgcaaag cgggtgaacc gccgccgaca
     2581 ctgatcgatt atcttccaaa aaacgcgcta ctggtcgtcg atgagagcca cgtgaccatc
     2641 ccacagctcg ggggcatgta caagggcgac cgctcgcgca aggagacgct ggtcgaatac
     2701 ggctttcgcc tgccttcggc gctggataac cgcccattac gcttcgatga atttgaacgt
     2761 ctgatgccgc aggctatttt tgtctcggca acacccggcc cgtatgaact gcaacgatcg
     2821 cagggtgtgg tcgtcgacca ggttgtgcgg cccaccggcc tgattgaccc ggaggtcgag
     2881 gtaaggccgg cgaccgccca agttgacgat ttgttgtccg aggtgcgggc gcgcgtcgcc
     2941 gccggcgagc gcgtgctggt caccaccctg accaagcgca tggcggagga tttgacggaa
     3001 tatctccatg aacacgatgt gcgcgtgcgc tacctgcatt cggacattga aaccatcgag
     3061 cgcgccgaga ttatccgcga cctgcgcgcc ggcaccttcg atgtgctggt cggcatcaac
     3121 ctgttgcgcg agggtctgga tatccctgag gtgtcgctgg tcgccatcct cgatgcagac
     3181 aaggagggct tcttgcgttc gacgcgctcc ctgatccaga ccattggccg cgccgcgcgc
     3241 catattcacg gcaaagccat tctttatgcc gaccggatga cggactcgat gcgtgctgcc
     3301 atcgacgaaa ccgagcgtcg tcgcctgaag cagattgcct ataacgaaga gcacggtatc
     3361 acgcccaagg gcatcatcaa ggcggtcaag gagatcatcg aaggcgtgac accggcacac
     3421 ccgttgtacg acaccaagga ttacggcaca aaatccgcgc acgccgggca tgagttcctg
     3481 catgccgccg aggaagaggc cgaatatgcc gccatggcgc cgagcgcgct ggccaagctg
     3541 ctcgacaagc tggaaaaaca aatgtacgcc catgcgcgca atctggagtt cgaaaaggcg
     3601 gcggcggtgc gcgacaggat ccatgcgatc cgctcacgca agttcggcgt gattgaaggc
     3661 cggggtcaga agcgcgggcg ctgagggggg atacgcgcct caaacttgta gccgcgcgcc
     3721 ggtttgtgct atttttccgc gcctgtttgc aggcgcgtag ctcagtggta gagcaccacc
     3781 ttgacatggt ggtggtcggt ggttcgatcc cactcgcgcc taccactaat taatccattc
     3841 cggccctgcc ggttgttgtg gagttgaact gaaacccgtt tacgcggcaa ttcgtagcgg
     3901 tcgccactga cgaatgggtc tcgttatgcc tgtcatcacg ctgccggacg gcagccgccg
     3961 tgaatacccc aaacccgtaa cggtcgccga gattgctgcg agcatcggcg ccggccttgc
     4021 gcgcgccgcg ctcgccgggc gcgtcaatga caagctggtc gatacctcgt tcctggtcga
     4081 ccaggacgcc acggtcgcga ttgtcaccga caagagcccg gagggcatcg aagtgatccg
     4141 gcactccacg gcgcatctgc tggcgcaggc ggtgaaggaa ttgttccccg aggctcaggt
     4201 aaccatcggg ccggtcatcg aaaacggttt ttactacgat ttcgccttca ggcgcggctt
     4261 caccgaggac gacttggcga aaatcgagca gcgcatgcat gagatcgcca agcgtgacta
     4321 tcccgtggcc cgtacggtca tgccgcgcga tgaggctatt aaattctttc gggacatggg
     4381 cgaagaatac aaggcccaga tcatcgcgga cattcccacc ggcgagcaga tatctctata
     4441 ccgtcagggc gagttcatcg acctgtgccg cgggccgcat gtaccctcaa ccgacaagct
     4501 caaggccttc aaactcatga aggtggccgg cgcctactgg cgcggcgatt cgcgcaacga
     4561 aatgctggcg cgcatctacg gcacggcctg gaccagcaag gaggcgctcg acgcctatct
     4621 gcatcagatc gaagaggccg agaagcgcga tcaccgcaag atcggacgtc aactcgacct
     4681 gtttcacatg caggaagagg caccgggcat gattttctgg catgagcgcg ggtggcgcct
     4741 gtaccagacc atcgtccagt acatgagcca gttgtggcgc gagcacggct accgcgaaat
     4801 tcgcacgccg cagctggtgg accggtcgct gtgggagaaa tccggacatt gggagaaatt
     4861 ccacagcgac atgttcatta cggagtcgga aaaacggctc tatgccgtca agccgatgaa
     4921 ctgcccctgt cacatccagg tgtataacca gggcctgaag agttaccgcg acctgccgtt
     4981 gcggctggcc gagttcggct cctgccatcg caacgagcaa tccggcgccc tgcacggcct
     5041 gctgcgcgta cgcaactttg tgcaggacga cgcccatatc ttctgtaccg agaatcagat
     5101 tcaagacgag gtttcagcgt tcattgatct gcttcaccgc gtttacgccg atttcggttt
     5161 cagggaggtc atctacaagc tctccacccg cccgcccaac cgcatcggtt cggatgagag
     5221 ctgggataag gcagaacatg ccttggaaca ggcgctgaac gccaaggggc tcaagtggga
     5281 tttgcagccg ggggaggggg cgttttacgg ccccaagatc gaattttcgc tcaaggacag
     5341 cttgggccgg gtctggcagt gcggcaccat ccaggtggat ttctccatgc cggggcggct
     5401 gggtgccacc tatatcgccg aagatggcag caagcaggtc ccggtgatgc tgcaccgggc
     5461 catcctcggg tcgctggagc gcttcatcgg cattctgatc gaggaacacg ccggggcgct
     5521 gccagcgtgg ctggccccgg tacaggctgt cgtcctgacc attaccgatg agcagcagga
     5581 ttacgccagg aagctcgaaa aaaccctggc caaacacggc tggcgggtcg agtctgactt
     5641 gagaaacgag aaaataggct ttaaaattcg cgaacatacc ttgcagcgca t
//
LOCUS       DBNGZE010000369         2248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1719213, whole genome shotgun sequence.
ACCESSION   DBNGZE010000369 DBNGZE010000000
VERSION     DBNGZE010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1719213"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..902)
                     /locus_tag="AC16FV_09455"
     CDS             complement(120..902)
                     /locus_tag="AC16FV_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR2850315.1"
                     /translation="MSLALFNPGRLGRKILGAADYWKGFGELLFYSLRHLGAIARPPV
                     RLVLQKQIYFTGIEALGAVTFIAALSGIVITTQVASLVGQNIALLSQVMLWTVVRELG
                     PLLSAIVIIARSSSATASELASMKIRGEIDSLRLMGISPGKYLVVPRIAGITFSVVAV
                     TFYFQITAIVMGYAFASLTGAMPFVKSISGVLSTLTMTEALVSLLKALVFGLVIAISS
                     CYYGLKATGTVTAVPRAATGAVMRNLLTIFLLDGVITYAFFV"
     gene            complement(913..1323)
                     /locus_tag="AC16FV_09460"
     CDS             complement(913..1323)
                     /locus_tag="AC16FV_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850316.1"
                     /translation="MNVKRWLFASLAVLVVIAVVEMVINNFLLMSLYQQTASVWRPMA
                     EIERMMWVFWLGYLVFAPFFVLIYTKGYEPAKPGLGQGLRYGLYMGVVLSVMQNLVWY
                     AVLPIPGVLAFYWFLGGMAESVAAGAVAGLIYRA"
     gene            complement(1363..>2248)
                     /gene="aroC"
                     /locus_tag="AC16FV_09465"
     CDS             complement(1363..>2248)
                     /gene="aroC"
                     /locus_tag="AC16FV_09465"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR2850317.1"
                     /translation="FEGKTTGTPIGLLIQNTDKRPQDYSKIMDRFRPGHADYTYMQKY
                     GLRDYRGGGRSSARETAMRVAAGAIAKKYLRERYGVTIRGYLAQLGPIKLELKDWNEV
                     ENNPFFCPDASKVAELEAYMDALRKEGNSIGARVNVVASHVPVGLGEPVFDRLDADIA
                     YAMMGINAVKGVEIGAGFRSVEQKGTEHRDEITPKGFLSNHAGGTLGGISSGQDIVVS
                     IALKPTSSIRLTGRTVNVQGEAVEIATTGRHDPCVGIRATPIAEAMLAIVLMDHALRH
                     RAQNADVKSPTPVIPGET"
BASE COUNT          454 a          699 c          700 g          395 t
ORIGIN      
        1 tttctgttcc tgcgagtcaa acagaatctt ggccgtggcg ccctgcttca gctcaaagga
       61 aagctcccgg atggaatcac cccgcacgtg atcaaagcgg atcatgcgac gcaccccggt
      121 tatacgaaga aggcataggt gatgacgccg tcgagcaaga agatcgtcag caggttgcgc
      181 atgacggcgc cggtggcggc gcgtggcacg gccgtgaccg tacccgtcgc cttcaggccg
      241 taataacagg agctgatggc gatgacgagc ccgaacacca gcgccttgag cagcgacacc
      301 agcgcctccg tcatggtcag cgtcgagagc acgccggaaa tgctttttac aaatggcatg
      361 gcaccggtga gggaggcaaa ggcgtagccc atgacgatgg ccgtgatctg gaaataaaaa
      421 gtaacggcca ccacggaaaa agtgatgccg gcgatgcgcg gcaccaccag atatttgccg
      481 ggtgaaattc ccatgagccg aaggctgtcg atctcgccgc ggattttcat gcttgccagc
      541 tccgaggcgg tggcggaact gctgcgcgcg atgatcacaa tggcggacag cagcgggccc
      601 aactcgcgca ccacggtcca gagcatgacc tgcgacaaca gcgctatgtt ctggcctacg
      661 aggctggcga cttgggtggt gatgacaata ccggacaacg cggcgatgaa ggttaccgcg
      721 ccgagcgctt cgatgccggt gaagtagatc tgcttttgca ggacaagtcg caccggcggc
      781 cgagcgatgg cgccgagatg ccgcaggctg taaaacaaca gctcgccgaa acctttccag
      841 taatcggccg caccgagaat ttttctgccg agccgtccgg gattgaacag cgcgaggctc
      901 acggcacgtg gttcaggcgc gataaatcag ccccgccacg gcgccggcgg cgaccgactc
      961 ggccatgcca ccgaggaacc agtagaacgc gagcacgccc gggatcggca gcaccgcgta
     1021 ccagaccagg ttctgcatga ccgacagcac cacgcccatg tacaggccgt aacgcaggcc
     1081 ttggcccagg ccgggtttgg ccggctcgta gcccttggtg tagatcagga caaaaaacgg
     1141 ggcgaagacc aagtagccca gccagaacac ccacatcatg cgctcgatct ccgccatcgg
     1201 ccgccaaact gaggcggtct gctgatacaa actcatgagt agaaagttgt tgatgaccat
     1261 ctccaccacc gcgatcacga ccaacaccgc aagactcgcg aacaaccagc gtttgacgtt
     1321 catggcactt cctccgttga gttagcgtaa gacgtgcgcg cttcaggtct ctcccgggat
     1381 caccggcgtg gggcttttga cgtcggcgtt ctgcgcacgg tggcgcagcg cgtgatccat
     1441 gagtacgatc gccagcatgg cctcggcaat cggcgtggcg cggatgccga cgcaggggtc
     1501 gtggcgcccg gtggtggcga tctcgacggc ctcgccctgc acgtttaccg tgcgcccggt
     1561 caggcggatg ctggacgtcg gcttgagcgc gatgctcaca acgatgtcct gcccggagga
     1621 aatgccgccg agcgttccgc cggcatggtt ggaaagaaaa cccttgggcg tgatttcgtc
     1681 gcggtgctcc gtgcccttct gttcgaccga gcggaagccc gcgccgatct cgacgccctt
     1741 caccgcgttg atgcccatca tggcgtaagc aatgtcggcg tccagccgat cgaacaccgg
     1801 ctcgccgagg ccaaccggca catggctcgc caccacgttg acgcgcgcgc cgatggaatt
     1861 gccttccttg cgcagcgcat ccatgtaggc ctcgagttcc gccactttcg aggcgtccgg
     1921 gcagaagaac ggattgttct cgacctcatt ccagtccttg agctccagct tgatcgggcc
     1981 cagttgcgcg agatagccgc ggatggtcac gccgtaacgt tcgcgcaggt atttcttggc
     2041 gatggcacca gcagccacgc gcatcgccgt ctcgcgcgcc gaggagcgcc cgccgccgcg
     2101 ataatcgcgc aggccgtact tctgcatgta ggtgtaatcg gcgtgccccg gacggaagcg
     2161 gtccatgatc ttggagtaat cctgcgggcg cttgtcggta ttctggatca gcaggccgat
     2221 cggcgtgccg gtggtcttgc cttcgaac
//
LOCUS       DBNGZE010000370         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_52155, whole genome shotgun sequence.
ACCESSION   DBNGZE010000370 DBNGZE010000000
VERSION     DBNGZE010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_52155"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(844..1200)
                     /locus_tag="AC16FV_09470"
     CDS             complement(844..1200)
                     /locus_tag="AC16FV_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HIR2850318.1"
                     /translation="MRSMRRDAILDIYQLLLAVFLFVSPWLFAFVNRTLRADDWLSAA
                     LVAVISFTALVAFREWQAWINFILGMWISASPWILGFQPPVAMRINLAIGLLISYLAI
                     FDLWLIHYGPLHEHRT"
     gene            1473..2189
                     /locus_tag="AC16FV_09475"
     CDS             1473..2189
                     /locus_tag="AC16FV_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HIR2850319.1"
                     /translation="MQPSEMQYLPLSPLYFSALVGIFLILLLLIQLGVLRYAYMRLGI
                     GSGAALMLLFGSLMGSYFNIPVAYLPGEQVVSGRDVYFFGMRYMVPMVVQWPGTLIAV
                     NVGGAVIPTVMSVHLLFKHQLWVTGSIAIAIVAFACHWMAQPVPGLGIALPVFVPALV
                     TSITALLLSRVHAAQLAYIAGSLGTLVGADLLNLGKIAGLGTPVASIGGAGTFDGIFL
                     TGILAVLLASLTGGRRPATS"
BASE COUNT          504 a          689 c          701 g          539 t
ORIGIN      
        1 cgggcgggcg atgggctgga agcatatcgg ctgagattgt cagttgatct atgcgactca
       61 ttgtcgcgaa ctttttcttc gagatgcgac gaccttgcga ttttgctcga caaccggttt
      121 ccctgtcacg tgacatgaac cctttgtaac gtgactgata ggttcagcca gcggcatatc
      181 cagtcgtcct tggcagcggc tcggagatcg ctgactggtt gctgcagatg caagtgccgt
      241 ttggattcgt cactggctac gatgatccct ttgaggcgag gcacggtaga gtcccgcttt
      301 tgcgcaagcc atttacagca ggtgagctag gcgagctact cgaaaagttg gtcgggcctg
      361 ctggcggatc ggacgaggct agccgaggtg tacagaaaat ccgcgctggc acttagcgga
      421 catttcagcc gtacctacgt ttgcccgcta ttggagtaat cgcggccatt atcggctgga
      481 tcggatttgc gcttctgata cttttcgcgc gtctctggac atttcaaggc aagaagcgag
      541 ttcggtgtag gtcggctcga accattttat tgatgcaaca catctaattc ggtctggggc
      601 cggaaacgag aaccagtcgc tcgcgagctt tttatgcgcg cggcgctcgg catccagaca
      661 gactttcaat ctgtctttga atggcttggc atgccctgcc gaaatagcgc ctcggcaact
      721 cggcgtgaca tcccaatttg gaacggcgtc cgccgtcgtt atccgaagcg ctatcggaag
      781 aacgaaaatt ggaatggcca tttcaacacc gtatggaact tggattggaa gattgaaaaa
      841 atactaagta cggtgttcgt gcaggggacc gtagtgaatt agccagaggt caaaaattgc
      901 caggtatgaa attaaaaggc caatggccaa attaatgcgc atcgcgactg gaggttggaa
      961 acccaaaatc caaggggagg ctgaaatcca catcccaagg ataaaattga tccatgcttg
     1021 ccattctcgg aatgcgacca gcgccgtgaa ggaaattaca gctaccagag cggcactaag
     1081 ccagtcatca gcgcgcaggg tgcggtttac aaacgcaaac agccaaggcg atacaaatag
     1141 aaaaaccgca agtagaagct gataaatatc caaaatcgcg tctcgcctca tactgcgcat
     1201 gatctagacc gccttgcagt ggcgccctct cgtttggagg tcgcccattt gctaaaacac
     1261 ggtggaagcc ctgaagttcc ctgcactttc tggttgccaa cgccgacgag gcgatcgagt
     1321 agtcatcggt cggcgtctaa tttacagaaa aaagcggaca tcgccgcctt actcaccgaa
     1381 tgtccgtggc cgcgcggccg actatattat caggcgtcat gtctgcggcg cggcgcttat
     1441 tcggcctcga acctggggga tactgagcaa caatgcagcc gagcgaaatg caatatctgc
     1501 cgctcagccc cctgtatttc tcggcgctgg tcggaatatt cctgatcctg ctgctgctca
     1561 ttcagctggg tgttctccgc tacgcctaca tgcggctcgg gatcggctcg ggcgcagctc
     1621 ttatgctgct gttcggctcg ctcatgggca gctacttcaa cattcctgtc gcttatctgc
     1681 ccggtgagca ggtcgtctcc ggtcgagacg tctacttctt cggcatgcgc tacatggtgc
     1741 cgatggtcgt gcagtggccc ggcacgctga tcgcggtgaa cgtcggcggc gccgtcattc
     1801 ccacggttat gtccgtccac ctcttgttta agcatcagct ctgggttacc ggctccatag
     1861 cgatcgcgat cgtcgccttc gcgtgccact ggatggcgca accggtcccc ggactcggca
     1921 tcgcgctgcc ggtattcgtc ccagctctcg tcaccagtat cacggcgctg ctactgtcgc
     1981 gggtccacgc cgcccagctc gcgtacatcg ccggcagcct cggcacgttg gtcggagccg
     2041 accttcttaa tctcggtaag atcgcagggc tcggcacgcc cgtcgcttcc ataggcggtg
     2101 ccggaacgtt cgacggcatc ttcttgacgg ggattctcgc ggtgctgctg gcgagcctga
     2161 ccggcgggcg tcgaccggcc acgtcatagg tgccggcctg agggcacacg cccgacatca
     2221 tttaggcgtt gttttgcggg agcgtatgcg cggtcggtct tggtcgagga ggtcgagatg
     2281 atcgccgagc caaaacacga agcccgccgg gacgatacgg ctcgcggcaa ccgcgagcgc
     2341 aagcggcatg aaaaagcgct cgacgaggcc ctcaagaata cctttccggc gtccgatccg
     2401 gtctcagtcg aacagccagt gctacccgcc gcg
//
LOCUS       DBNGZE010000371         1713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1511038, whole genome shotgun sequence.
ACCESSION   DBNGZE010000371 DBNGZE010000000
VERSION     DBNGZE010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1511038"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16FV_09480"
     CDS             complement(<1..413)
                     /locus_tag="AC16FV_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR2850320.1"
                     /translation="MTIATARTTVVRPVIAVINSERTLEGGGFPVRRPFPTTQLMQVD
                     PFLLLDHLGPVTWGPGEGIGAPDHPHRGFETVTYLLSGEMQHKDSAGHSGSLRPGDVQ
                     WMTAGAGVVHSELPSDAFMKNGGTLHGFQVWVNLPT"
     gene            complement(410..1387)
                     /locus_tag="AC16FV_09485"
     CDS             complement(410..1387)
                     /locus_tag="AC16FV_09485"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR2850321.1"
                     /translation="MGYLIKGEWHEGWYDTSKTAGEFIRADSQFRDTVTANGASGYPA
                     EAGRYHLYVSLACPWAHRTIIYRKLKQLEDIISMSVVDPVITEGGWAFRDGFTDTVNS
                     FAFLREAYTRTRPDYSGRVTVPVLWDKQTGRIVNNESAEIIRMLNSEFNAFTTVKTDF
                     YPAALRREIDETNAFVYERINNGVYKAGFAGSQRVYESAVTRLFTALDEIETRLGQSR
                     YLVGDTLTEADWRLFTTLIRFDAVYVTHFKCNLQRIEDYPNLSNYLRDLYQVPGIAEA
                     VDFDHIKRHYFLSHRQLNPLGLVPKGPILDFSTVHNRERLMARDARRIS"
     gene            complement(1408..>1713)
                     /locus_tag="AC16FV_09490"
     CDS             complement(1408..>1713)
                     /locus_tag="AC16FV_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2850322.1"
                     /translation="VKFFEALGYPGFLAYVVFGAELVGGTLLVLGVYTRRVSLALIPI
                     LIGAAMVHIPNGWVFSAPNGGWEYPVFLAIASAVQALLGDGALALRASAASPARQPA"
BASE COUNT          335 a          504 c          542 g          332 t
ORIGIN      
        1 gtcggcaggt ttacccacac ctggaaaccg tgcagggtgc cgccgttttt catgaaggcg
       61 tcggagggca gttccgagtg caccacgccg gcgccggccg tcatccactg tacgtcgccg
      121 gggcgcagtg agccgctatg accggccgag tccttgtgct gcatctcgcc ggagagcagg
      181 taagtcacgg tttcgaagcc gcgatgcggg tggtccggcg cgccgatgcc ttcccccgga
      241 ccccaggtta cgggacccag atgatcgagc aacaggaacg ggtctacttg catcagttgc
      301 gtcgtgggga acggtctccg cacggggaaa ccaccacctt ctaaagtcct ttcactgtta
      361 atgacagcaa tgacgggtcg gactacggtg gtcctggcgg ttgctattgt catgaaatcc
      421 tccttgcatc gcgcgccatc aggcgttcac ggttgtgcac tgtgctgaag tcgagtattg
      481 gacccttcgg caccaagccc aagggattga gctgccggtg actcaggaaa tagtggcgct
      541 tgatgtggtc gaaatcgacc gcctccgcga tgcccggaac ctggtacaga tcgcgcagat
      601 agttcgacag gttcggatag tcctcgatgc gctgcaggtt gcatttgaaa tgcgtgacgt
      661 acacggcatc gaagcggatc agcgtcgtga acaggcgcca atcggcctcg gtgagcgtat
      721 cgcccacgag ataacgcgat tgcccgaggc gtgtttcgat ctcgtcgagc gcggtgaaca
      781 ggcgcgtgac cgcggattca tacacccgct ggctgccggc gaagccggcc ttgtacacgc
      841 cgttgttgat gcgctcgtat acgaaggcat tggtctcgtc gatttcccgg cgcagggccg
      901 caggatagaa atcggttttc acggtagtga aagcgttgaa ctcgctgttg agcatgcgga
      961 tgatctcggc cgattcgttg ttgacgatcc ggccggtctg cttgtcccac agtaccggca
     1021 cggtcacgcg cccggaatag tcaggcctgg tgcgcgtata ggcctcgcgc aggaaggcaa
     1081 aactgttaac cgtatcggtg aaaccgtcgc ggaatgccca gccgccttcg gtgatgaccg
     1141 ggtccaccac cgacatcgag attatgtcct cgagctgctt gagcttgcga taaatgatcg
     1201 tgcgatgcgc ccacgggcag gcgagtgaaa cgtaaagatg ataccgtccg gcctcggccg
     1261 gatagccgct ggcgccgttg gccgtgaccg tgtcgcgaaa ctggctatcg gcgcggatga
     1321 actcgccggc cgtcttgctg gtgtcatacc agccttcgtg ccactcgcct ttgatcaaat
     1381 agcccatggg atttctcctt tgccttgtca ggccggttgc cgggcgggac tggccgccga
     1441 ggcgcgcaag gccagggcgc cgtcaccgag cagggcctgc actgcagacg cgatcgcgag
     1501 gaacacagga tactcccatc cgccattggg cgcgctgaac acccagccgt tgggaatgtg
     1561 caccatcgcc gcgccgatca gaatggggat gagggcaagt gaaacccggc gcgtatacac
     1621 gccgagcaca agcagtgtgc cgccgaccag ttcggcaccg aatacgacat aggccaggaa
     1681 accggggtat ccgagcgcct cgaaaaattt gac
//
LOCUS       DBNGZE010000372         6063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1580780, whole genome shotgun sequence.
ACCESSION   DBNGZE010000372 DBNGZE010000000
VERSION     DBNGZE010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1580780"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC16FV_09495"
     CDS             <1..340
                     /locus_tag="AC16FV_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR2850323.1"
                     /translation="YENAMIIELRKLGFDVKQQFPITVHYEGKPVGEYFADILVENRV
                     ICELKSAQQLAPEHEMQLVNYLAATGHNTGLLLNFGNSVMVKRKFREYRKPRESITPE
                     VNPVNPEKSC"
     gene            345..1586
                     /locus_tag="AC16FV_09500"
     CDS             345..1586
                     /locus_tag="AC16FV_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR2850324.1"
                     /translation="MNSNVSKSDAVEDKLVAARTWLIIDKPFLGALVLRLPLVEANPK
                     WCQTTATDARSFYYNRRYIEALSFEQTKFILAHEALHCALSHFARREHRDKRRWDLAC
                     DFAINPLLVADGLKPAPDALILDAYKDMTAEEIYPCIQENTDEKPMDQHLYDQDDRPH
                     GPGEQGGGQGGEGQDQNDDNGGGSQGETDPQANGAPQPPPLSAPERDQLHKQWQQRLA
                     SAAQQAIQAGKLGGSMARIVDHLLQPQLPWRMLLAHYMTRASRDDYSFAHPSRREGTA
                     ILPGMRSQHIDLIVVLDTSGSISDEEMQEFVSEVNAIKGQVGARITLHACDERLAQDG
                     PWTFEPWEEIELPKQWHGGGGTKFTPVFEWAENQDRPPQLLLYFTDAKGEFPKREPSY
                     PVLWLVKGKEKVPWGQRIQLN"
     gene            1751..2914
                     /locus_tag="AC16FV_09505"
     CDS             1751..2914
                     /locus_tag="AC16FV_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrS"
                     /protein_id="HIR2850325.1"
                     /translation="MELSSEDLLRLNVLLANDIEAIRIDEQSMTVYGLARGNEARVPL
                     NPNCRPEKYLRLVREMLSSHVLGSPGGYPVFLQRWTRMGQARETRLDKLLLLGEPEAV
                     TAVAGAPGLSDDLARRTWWAQPTSDIARRMLDKQAVVQGTMGRELADYLAKHLPFESD
                     PMLVIATIRLILQPGLIDAEARDRIFRLGTHRNAYHIGFLEALPDELPEPLPARPDHA
                     NYRDRLAALAADGNALASFLAKLLDSPGQTFLAVSETQLQHPVDKYTVAKLMDIIGNY
                     FRPALGTQKPAGEISVAVACAEKLMAASDGETAALLDSLPELREEIAAMLTLAHTGEA
                     LVTPIIAASTASGTLLRRKLEPVLEPLLQQYAVLRKTAQAPAGGRAARRVSGK"
     gene            complement(3228..3536)
                     /locus_tag="AC16FV_09510"
     CDS             complement(3228..3536)
                     /locus_tag="AC16FV_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR2850326.1"
                     /translation="MSDEKTSPLESKEYQILKALKLVLTDVVKDTATQPGLKHPLSDR
                     TIEGIRQCLKLISARERELIENSGKTMGMRPYYADEPKKNVVVPITKITRHKKDPEKK
                     "
     gene            complement(3611..4282)
                     /locus_tag="AC16FV_09515"
     CDS             complement(3611..4282)
                     /locus_tag="AC16FV_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR2850327.1"
                     /translation="MRSNRLSSARSSAAALGLCALFAVGAAARAEVYVYELPDGSRMI
                     TDHPINARHSRVVRVAPDVKGAGRLAAQKNSPVFRENSSTYDRLIRRYADEHGVDFAL
                     VKAIMQAESSFNPYAVSSKGARGLMQVMPETASRYGVRDIYNPVENIRAGVEHLRYLS
                     GVFNNKLYLVIAAYNAGENAVKQHQGIPPYEETQMYVRKVLQYQRQYSPRYRYTTLAA
                     NPAGG"
     gene            complement(4463..4825)
                     /locus_tag="AC16FV_09520"
     CDS             complement(4463..4825)
                     /locus_tag="AC16FV_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2850328.1"
                     /translation="MQKSRYASVESFVTKDGSIIRELMHPSIHGNVNQSLAEAIVPVG
                     QGTQLHRHLHSEEIYHVTEGEGRMVLGDQKFAINPGDTICIPPGTPHRVDNTGPTPLK
                     ILCSCSPPYSHADTELLK"
     gene            complement(4842..5546)
                     /gene="pgl"
                     /locus_tag="AC16FV_09525"
     CDS             complement(4842..5546)
                     /gene="pgl"
                     /locus_tag="AC16FV_09525"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR2850329.1"
                     /translation="MNAPTPTTRWQVFPDAAALAGQAAALILDAAREAIAARGVFHLV
                     LAGGHTPKSAYERLRDCGANWVKWQIYFGDERCLPRGDPGRNDTMARRAWLDHIRIPP
                     VNVHPIPAEHGAEKGAQRYAEMLRRAPQFDLVLLGLGEDGHTASLFPGQQYGLEKDDM
                     AVVAVRVAPKPPLERVSLSIARLSRARRALFLVGGETKRDAVKLWRAGADIPARHITP
                     TQGVDVYVDRAAWPEA"
     gene            complement(5550..>6063)
                     /locus_tag="AC16FV_09530"
     CDS             complement(5550..>6063)
                     /locus_tag="AC16FV_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR2850330.1"
                     /translation="AKFYIDNWRWRNVPFYVRTGKRLTENSSLIAIRFRHPPQLLFHE
                     AGAAHIDPNWVLLSMQPTEGMHIELHAKEPGLGMNTRIVRLNASYRQSPATTMEAYKT
                     LLLDVVEGDRSLFLRFDEVEWAWRVVDPILRDWSRRREYIATYPGGSWGPREAGRLFE
                     REDQFWRNEI"
BASE COUNT         1225 a         1869 c         1792 g         1177 t
ORIGIN      
        1 ttatgaaaat gcgatgatca ttgaactgcg gaaactggga ttcgatgtta aacagcaatt
       61 tcctataaca gtacactatg aaggaaaacc cgtcggagaa tatttcgctg acattcttgt
      121 cgagaacaga gttatctgcg aactgaaatc tgctcaacag ctagcaccag aacatgaaat
      181 gcagctggtc aactacctgg cggcaacagg ccacaacacg ggtttgcttt tgaactttgg
      241 taatagcgtt atggtaaagc ggaaattccg cgagtatcgc aagccacgtg agtcaataac
      301 tccagaagta aatcctgtta atcctgaaaa atcctgttaa tcctgtgaat tcaaacgtct
      361 ctaaaagcga cgccgtcgaa gacaaactcg ttgcggcgcg cacctggctg attatcgaca
      421 agcccttcct cggtgcactg gtgttgcgcc tgccactggt cgaggccaat cccaagtggt
      481 gccagaccac tgccaccgac gcgcgctcgt tctattacaa ccggcgctat atcgaagcat
      541 tatccttcga gcagaccaaa ttcattctcg cgcacgaggc tctgcactgc gcgctgtcgc
      601 acttcgcgcg ccgcgaacac cgcgacaagc gccgctggga cctggcctgc gatttcgcga
      661 tcaatccgct gctggtggcc gacgggctga aaccggcgcc ggatgcgctc atcctcgacg
      721 cctacaagga catgaccgcg gaggagattt atccctgcat ccaggaaaac accgacgaaa
      781 aacccatgga ccagcacctg tacgatcagg acgacaggcc gcacggtccc ggtgaacagg
      841 gtggcggcca aggcggtgag gggcaggacc agaatgatga taacggtggc ggcagccagg
      901 gtgaaacaga tccgcaggcc aatggcgcgc cgcaaccgcc gccgctgtcc gccccggagc
      961 gcgatcagct gcacaagcag tggcagcagc gcctggcctc ggccgcgcaa caggccatcc
     1021 aggccggcaa gctcggtggc tcgatggcgc gcatcgtgga tcacctgctg caaccgcaac
     1081 tgccgtggcg catgctgctg gcgcattaca tgacgcgtgc ctcgcgcgac gactacagtt
     1141 tcgcgcatcc gtcacgacgc gagggcacgg cgatcctgcc cggcatgcgc agccagcaca
     1201 ttgacctcat tgtggtgctc gataccagcg gctctatctc ggacgaagag atgcaggaat
     1261 ttgtgagcga ggtgaatgcc atcaagggcc aggtcggcgc gcgtatcacc ctgcacgcct
     1321 gtgacgagcg actggcgcag gatggcccct ggaccttcga accgtgggag gaaatcgaac
     1381 tgccgaaaca atggcatggc ggcggtggca cgaaattcac gcctgtattt gaatgggccg
     1441 agaaccagga ccggccgccg cagctgctgc tctatttcac cgacgccaag ggtgaattcc
     1501 ccaagcgtga gccatcctat ccggtgctgt ggctcgtcaa gggcaaggag aaagtgccat
     1561 ggggccagcg gattcaattg aactaattta attaacagga tttacaggat ttttcaggat
     1621 taacaagatt aaatctttta aaatttttaa atctattcaa cataaaatct gaatgcggtt
     1681 atctgttatt ctcaatattc ctgtaaatcc tgcgtaatcc tgttaatcct gtttattctt
     1741 tacttataaa gtggaactct ccagcgaaga cctgctgcgg ctgaacgtgc tgctcgccaa
     1801 cgacatcgag gcgatccgca tcgacgagca gagcatgacg gtctatgggc tggcgcgcgg
     1861 caacgaggcg cgcgtgccgc tcaaccccaa ctgccgtccg gaaaaatacc tgcggctggt
     1921 gcgcgaaatg ctctcaagcc acgtgctcgg ctcgcccgga ggttacccgg tgttcctgca
     1981 acgctggaca cgcatgggcc aggcgcgcga gacccggctg gacaaattgc tcctgctcgg
     2041 cgaacccgag gcggtgaccg ccgtggccgg cgcgccgggc ctctccgacg atctggcgcg
     2101 gcgcacctgg tgggcccaac cgacgtccga catcgcgcgg cgcatgctcg acaagcaggc
     2161 cgtggtgcag ggaacgatgg gccgggaact cgcggattat ctggcgaaac atctgccgtt
     2221 cgaatccgac ccgatgctgg tcatcgccac gatacggctc attctccagc ccggtttgat
     2281 cgacgccgaa gcgcgcgacc gcatcttccg gctgggcacg caccgcaacg cctatcacat
     2341 cggatttttg gaagcgctcc cggacgaact tcctgagccc ctgccggcgc gtccggacca
     2401 tgcaaattat cgggaccggc tcgcggcgct ggcggcggat ggcaacgcgc tggcgtcatt
     2461 cctcgccaaa ctgctcgaca gtccggggca gacctttctc gccgtctccg agacgcaact
     2521 gcaacacccg gtggacaaat acaccgtggc caagctgatg gacatcatcg gaaattattt
     2581 caggccggcg ctgggcacac aaaaaccggc cggcgaaatc agcgtggccg tggcttgcgc
     2641 ggaaaaattg atggcagcat cggacggtga aaccgccgcg cttctggatt ccctgccgga
     2701 attgcgggag gaaattgccg ccatgctcac gctcgcgcac accggcgagg cactggtcac
     2761 gcccatcatc gccgcgtcta ccgcgagcgg gaccctgctg cgccgcaaac tcgaaccggt
     2821 gctggagccg ttgctccaac agtacgcggt gttgcgtaaa accgcacaag caccggccgg
     2881 cggcagagcg gccagacggg tttccggaaa gtagtcgaga gttttaaatt actaacaaaa
     2941 aatagacagg attaatcagg gcgagccgga aacagacgaa gacacttaac gtgtcttcgt
     3001 cccggcgagc gccccgccac ggatggcggg gccgagtatg tcgaagtttt catggtcacg
     3061 ccagggacgg caggcaggcc ttccctggcg tcgcgacaga gacttcgatt caccccagcc
     3121 cgcacttccg tgtgcgggcg cttagcggcg ccgatgtacg ttaagtacat cggcgacatc
     3181 tccgcttcgc ccagtgaatc ctgttaattc ttttttgttt tatattttta cttcttctcg
     3241 gggtctttct tgtggcgggt gatcttggtg atcggcacca cgacgttttt cttcggctcg
     3301 tccgcgtagt aaggacgcat gcccatggtc ttgccggaat tctcaatcag ctcgcgttcg
     3361 cgcgcggaaa tcagcttgag gcactgacgg ataccttcga tggtgcgatc agacagcgga
     3421 tgcttcaggc ccggctgcgt cgccgtgtcc ttcacgacgt cggtcagcac cagcttcagg
     3481 gctttcagaa tctggtattc cttgctttcc agcggggagg ttttttcgtc gctcatgtcc
     3541 tttgctcggc caatgcgcag ggaaaccgca caaccggccg caggttacag ggaatgccgc
     3601 ggcttggcaa tcagccgccg gcgggattgg cggcgagcgt ggtgtagcgg tagcgaggac
     3661 tgtactggcg ctggtactgc aagaccttcc tgacgtacat ctgcgtttcc tcgtacggcg
     3721 gaatgccctg atgctgcttc accgcgttct cgccggcgtt gtaggccgcg atgacgaggt
     3781 agagtttgtt gttgaacacg ccggacagat atctcagatg ttcaacgccg gcgcggatgt
     3841 tctcgaccgg gttgtagata tcgcgcacgc cgtagcggct cgccgtttcc ggcatcacct
     3901 gcatcaggcc gcgcgcgccc ttgctcgaca cggcgtaggg attgaaagac gattccgcct
     3961 gcatgatggc cttgaccagc gcgaaatcca cgccgtgctc atcggcgtaa cgccggatga
     4021 gacgatcata ggtcgaggaa ttctcgcgaa acaccggact gttcttctgc gccgcgagcc
     4081 tgcccgcgcc cttcacgtcg ggcgccacgc gcacgacgcg cgaatgcctg gcgttgatcg
     4141 gatgatcggt gatcatgcgc gaaccatccg gcagctcgta cacatatacc tcggcccgag
     4201 ccgctgcgcc cacggcaaac aacgcgcaca gcccgagggc tgccgcgctg cttcgcgccg
     4261 atgacagtct gttactccgc atccgtcaga attttccttt taaatccacg gatatagcct
     4321 gcaactcccg tgccgatggt ccgcggtacg caagacgcct aacttcctgc cctgacacgg
     4381 ttttttttga cgcatgctgg tcacgccagc ttccggaaga cggaaatcgt cagacaaggg
     4441 tcgtaatgcg gccgggtaag gtctatttca gaagctcggt atcggcgtga gagtacgggg
     4501 gcgaacaact gcacagaatt ttcaatggcg tcggcccggt gttgtccacc cggtgcggcg
     4561 tcccgggcgg gatgcagatg gtgtcacccg gattgatggc gaacttctga tcgcccaaca
     4621 ccattcggcc ttcaccttcg gtgacgtggt aaatctcttc gctgtgaaga tgccgatgca
     4681 actgcgtccc ctgccccacc ggcacaatgg cttccgccag actctggttc acattgccat
     4741 ggatactcgg gtgcatcagc tcgcggatga tggagccgtc tttggtgacg aaggactcga
     4801 cgctggcgta tcggcttttc tgcatggcgc acctgtcagt attatgcctc cggccacgcc
     4861 gcccggtcga cgtacacatc aacgccttga gtgggcgtaa tgtgccgcgc cggaatatcc
     4921 gccccggcac gccacaactt caccgcgtcg cgcttggttt cgccgccaac cagaaacagc
     4981 gcccgccgcg cgcggctcag gcgcgcgatg ctcaggctta cgcgttccag cggcggtttc
     5041 ggcgccacgc gcaccgccac aacggccata tcgtcttttt ccaaaccata ctgctggccg
     5101 ggaaacaggc tggcggtgtg gccgtcctcg cccagcccca gcagcaccag atcgaactgc
     5161 ggcgcgcgac gcagcatctc agcgtaacgc tgcgcgccct tttccgcgcc atgctcggcc
     5221 gggatgggat ggacgttcac cgggggaatc cggatgtgat cgagccaggc gcggcgcgcc
     5281 atggtgtcgt tgcgcccggg atcgccgcgc ggcaggcagc gttcgtcgcc gaaataaatc
     5341 tgccacttga cccaattcgc gccgcaatcg cgcaggcgct cgtaagcgct cttgggcgta
     5401 tggccgccgg ccagcaccag atgaaacacg ccgcgtgccg cgattgcttc gcgcgcggcg
     5461 tcgaggatca gcgccgccgc ctgccccgcg agcgccgcgg catccggaaa tacctgccag
     5521 cgcgtcgtcg gtgtcggggc attcacgcgt cagatctcgt tgcgccagaa ctggtcttcg
     5581 cgctcgaaca gacggccggc ctcgcgcggg ccccagctcc cgcctggata agtggcgatg
     5641 tactcgcggc gacgcgacca gtcgcgcagg atgggatcaa ccacgcgcca cgcccattca
     5701 acctcgtcga agcgcaggaa cagactacgg tcgccctcga cgacatccag cagcagcgtc
     5761 ttgtacgctt ccatcgtcgt cgccgggctc tggcggtaag aggcgttaag ccgcacgata
     5821 cgcgtgttca tgccgagacc cggttccttg gcgtgcaact cgatgtgcat gccctcggtc
     5881 ggctgcatcg agagcaatac ccagttgggg tcgatgtgcg ccgcgccggc ctcgtggaac
     5941 aacagttgcg gcggatggcg gaagcggatc gcaatgagcg aggaattctc ggtcagacgc
     6001 ttgccggtgc gcacgtaaaa cggcacgtta cgccagcgcc aattgtcgat atagaacttg
     6061 gcc
//
LOCUS       DBNGZE010000373         7008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1927365, whole genome shotgun sequence.
ACCESSION   DBNGZE010000373 DBNGZE010000000
VERSION     DBNGZE010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1927365"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16FV_09535"
     CDS             complement(<1..413)
                     /locus_tag="AC16FV_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR2850331.1"
                     /translation="MRSHFCGELNESLIGQRVTLCGWAHSRRDHGGVIFIDLRDREGV
                     VQTVFDPSKKEPFAIAESVRTEFVLRIQGSVRARPVGTENPHLRTGRVEVVADSIEIL
                     NRAEALPFQLDEEGLSEPVRLTYRYLDLRREPMQK"
     gene            complement(451..1089)
                     /locus_tag="AC16FV_09540"
     CDS             complement(451..1089)
                     /locus_tag="AC16FV_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR2850332.1"
                     /translation="MAALRRYLMAGLLVWVPLGVTLLIVAFLVDLMDQTLLLLPASIQ
                     PENLLGFRIPGLGAVLTAVVVFVTGMIVTNLFGHQLFTIGENLLQRIPLVRSIYASVK
                     QVTESMFSSGKSFRKVVLVEYPRHGMWSLAFQTGAGAAEVNRKTGREIVNIFIPTTPN
                     PTSGFFLMVPREDVIELDMSVDQGLKMLLSVGVVVPENKKDEIIHQHARRAS"
     gene            complement(1130..1498)
                     /locus_tag="AC16FV_09545"
     CDS             complement(1130..1498)
                     /locus_tag="AC16FV_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR2850333.1"
                     /translation="MRYRRSRDIIRLVCIILRPHTLGARFFGRSCMPIYEYECGSCGH
                     RLEAIQKMSDPALKDCPACGQSTLKKLISAAGFQLKGTGWYATDFKGGGKKEKSDSSD
                     TPPKKKAGGGCGSGGCGCAH"
     gene            1843..2163
                     /locus_tag="AC16FV_09550"
     CDS             1843..2163
                     /locus_tag="AC16FV_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR2850334.1"
                     /translation="MANNKPGKKPTTKSEIYAHIAEKTNLKRKDVVAVFDTLTSLIER
                     DLKKGPGIFNLVGMMKIKMVYKPAVPARKGINPFTKEEVIFKAKPARNVVKCQPLKML
                     KDMV"
     gene            complement(2383..2679)
                     /locus_tag="AC16FV_09555"
     CDS             complement(2383..2679)
                     /locus_tag="AC16FV_09555"
                     /inference="COORDINATES: protein motif:HMM:NF042799.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HIR2850335.1"
                     /translation="MPMYEEPVVGAFYEDLEEDGRTFEVLAFDENDGTIEIQYSDGTT
                     DEIDLDDWYGMDLEQLESEEEDDEDDVDDSEAPDDDDTDLDDDDELDEDEEEEE"
     gene            2972..3319
                     /locus_tag="AC16FV_09560"
     CDS             2972..3319
                     /locus_tag="AC16FV_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR2850336.1"
                     /translation="MTTDCLFCKMVSGQIKPNTVYEDGDVLAFKDINPQAPVHVLVIP
                     KKHIATLNDLDTGHIELTGKLIRVAARIARESGIAESGYRIVMNCNADAGQSVFHVHL
                     HLLGGRPMHWPPG"
     gene            complement(3376..3846)
                     /gene="ssb"
                     /locus_tag="AC16FV_09565"
     CDS             complement(3376..3846)
                     /gene="ssb"
                     /locus_tag="AC16FV_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR2850337.1"
                     /translation="MARGVNKVILIGNLGRDPEVRYSPNGSAVANVTLATSESWKDKN
                     SGEKQEKTEWHRVVFFGRLAEIAGEYLKKGSQVYVEGRLQTRKWQDKEGHERYTTEIV
                     ANEMQMLGSRGGAGVPSDNFNQDQPSAAENSGPAGAKKAGNTAVAGDFDDDIPF"
     gene            complement(4143..5516)
                     /locus_tag="AC16FV_09570"
     CDS             complement(4143..5516)
                     /locus_tag="AC16FV_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2850338.1"
                     /translation="MSSPQDRMSPLERRASAGLASIYGLRMLGMFLILPVFAVYAEHL
                     PGGENHSLIGLALGVYGLTQALLQLPFGMASDRLGRKRVIYFGLVLFALGSFIAAAAD
                     DIYMVIIGRTIQGAGAINAAVTALLADLTREEHRTKAMAMVGGTIGLTFALSLILGPA
                     LYHLISIPGIFAMTGVLAMLAILMMKFIVPDPRQSHFHSDAEATPAGLLGVLRDRELA
                     RLNFGIFALHVVQMAMFVVVPFALRETGGLDANHHWQVYLPVLAISVVFMVPLIIYSE
                     KKAQAKPVFVGSIALMLAVEFAFVPALQHFWGLVTVLTLYFTAFNVLEASLPSLVSKI
                     APVRAKGTALGVYNTSQALGLFVGGVMGGVLSDHFGAAAVFEFGAILIGIWLYLAYGM
                     KAPPAVKNLMFHINTLNTEDARRLENQLTRVQGVTEATVMADEGVAYLKVDSRRFDEP
                     ALRRLLP"
     gene            5601..>7008
                     /locus_tag="AC16FV_09575"
     CDS             5601..>7008
                     /locus_tag="AC16FV_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007973840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990391 - DNA repair complex [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrA family protein"
                     /protein_id="HIR2850339.1"
                     /translation="METIRIRGARTHNLKNIDVDLPRDKLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLALMEKPDVDLIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIYDYLRLLYARVGEPRCPEHGVTLSAQTVSQMVDHVMTLPEGTKLMLLAPLIEERKG
                     EHLHILERLRTQGYIRARIDGTVVSLDEAPELKKNFKHTIEAVVDRAVVRPGQEQRLA
                     ESFETAANLGEGLIRVVVLDEQNNSTQEIVFSARYACPHCGYSLAELEPRLFSFNNPA
                     GACPECDGLGVRQFFDPARVVADPTLSLPAGAIRGWDRRNAFYFHMLESLAKHYKFDL
                     DAPFEKLPKKVRDVILHGSGDEIISFTYFAEQGPRAKRKHAFEGVIPNIERRYRESES
                     LSVREELAKFLSHQPCETCNGSRLKREARHVFVEGKALHEITQLPVANALDFFQGLRL
                     TVSRGEIAAKIVKEVANRL"
BASE COUNT         1533 a         2153 c         1951 g         1371 t
ORIGIN      
        1 ttcttctgca tcggctcgcg ccgcagatcg agatagcggt aggtcagacg caccggctcg
       61 gacagcccct cctcgtcgag ctggaacggc agcgcctcgg cgcggttgag gatttcaatg
      121 ctgtcggcga cgacttccac tctgccggtg cgcaaatgcg gattctccgt gccgaccggc
      181 cgcgcgcgca cgctgccctg gatgcgcagg acgaattccg tgcgcaccga ttcggctatg
      241 gcgaaaggtt cttttttcga tggatcgaac acggtctgca ccacgccttc gcgatcgcgc
      301 aggtcgatga aaatcacgcc gccgtggtcg cgccggctgt gggcccagcc gcacagggta
      361 acgcgctgac cgatgaggga ttcgttgagt tcgccgcaaa aatgactgcg catgattgtc
      421 tgtttcttgt gtgtgaaaaa tggaacgcgc tcaggaggcc cggcgcgcgt gctgatggat
      481 gatttcgtct tttttatttt ccgggaccac cacgccgacg gacagcagca ttttaaggcc
      541 ctggtccacg ctcatgtcga gctcgatcac gtcctcccgc ggaaccatga gaaaaaaacc
      601 ggaggtggga ttcggcgtgg tggggatgaa gatgttcacg atctcccgcc cggtcttgcg
      661 attgacctca gccgcccccg ccccggtctg aaacgccagc gaccacatgc catggcgcgg
      721 gtattcgacc aacaccacct tgcgaaacga cttgccggag gaaaacatgc tctcggtcac
      781 ctgcttgacc gaggcgtaga tcgaacgcac cagcgggatg cgctgcaaca ggttctcgcc
      841 gatggtgaaa agctggtggc cgaataggtt ggtgacgatc atgccggtca cgaacacgac
      901 caccgccgtc agcaccgccc ccaagcccgg aatccggaag ccgagcaggt tttccggctg
      961 aatgctcgcg ggcagcagga gcagcgtctg gtccatcagg tccaccagga acgcgacgat
     1021 gagcaaggta acgccgagcg ggacccagac cagcagcccg gccatcagat aacgtcgcag
     1081 cgcggccatt cttatctcag gacgaacgaa gtagggcttc actcacgcct caatgggcgc
     1141 agccgcaacc gccgctgccg caaccgccgc cagccttctt tttcggtgga gtgtccgaag
     1201 agtcagattt ttccttcttg ccgccgcctt tgaaatcggt ggcataccag ccggtgccct
     1261 tgagctggaa gccggcggcg gaaatcagtt tcttgagagt ggactggcca catgccggac
     1321 agtccttgag ggccgggtcg ctcatcttct ggatggcctc caagcggtga ccacaagacc
     1381 cgcattcata ttcataaatc ggcatacagg atctcccgaa aaaacgcgcc cccaaagtat
     1441 gggggcggag aataatacaa acaagccgaa ttatatcccg ggacctcctg taacgcatta
     1501 tttttaaagc attgtctggc ggcgtggcct tgtgaagacg cgcgtgggta tcccagaacg
     1561 ctgccagggc gtcggcaatg ttccagcgtg acaccgcgct ccctctttcc cggctggacc
     1621 gtgaataagg gtgcggggtg ggtttccccg tcacggagga ccgggtcagg atcacgatag
     1681 cggagcggcg tggtcagaat cagtcgccgt ggtgcggtac cgggaacccc ggaatttcag
     1741 gggaaaaaat ggattccgct cttctatccc cctgcttttt ccgataaatt atgcgcgttt
     1801 aaaggaaccc cttaacccaa ccttccactt caggagcatt acatggcaaa caacaaaccg
     1861 ggaaagaaac cgacaacgaa gtccgagatc tacgcccata tcgctgagaa aaccaacctc
     1921 aagcggaagg acgtcgtggc ggttttcgac acgttgacca gcctcatcga acgcgatctg
     1981 aaaaagggac cgggaatctt caatctcgtt ggcatgatga agatcaagat ggtctacaag
     2041 ccagccgtac ccgcgcgcaa gggtatcaac ccgttcacca aggaagaggt tattttcaag
     2101 gccaaacccg cgcgtaacgt ggtcaagtgc cagccgctga aaatgctgaa ggacatggtg
     2161 taaacggaaa cacgcaagga tttaagcgtt tctgaaaaca cctgtttgat gcgcccttgg
     2221 cgcacgggat gttcgtcggg ggtgtaacgg attcgggaca acgatccacc ggatcgttgt
     2281 cccgtcagtt tggttgatcc ggtgacaatt cgttgaaccc tgaaaacagg ttcaatataa
     2341 aaacaacatt actgtttttc gcctgccggc aaacggccgg ttttactcct cttcttcctc
     2401 gtcttcgtcc agttcatcat catcatcaag atcggtatcg tcatcatcag gggcttcgct
     2461 gtcgtcaacg tcgtcctcat catcctcttc ctcggattcc agctgctcca gatccatacc
     2521 gtaccagtca tccagatcga tctcgtcggt agtaccatcg ctatactgga tttcgatcgt
     2581 cccgtcattt tcatcgaagg cgagcacttc gaaggtgcgg ccatcctctt ccaggtcctc
     2641 gtaaaaggcc ccgaccaccg gctcttcata cataggcatt ttcaatctcc ccgcgaaaaa
     2701 cttggctgtc ttcccagaaa tatatagacg gcattttgcg ctccgccact gcttccgccc
     2761 gccgctcttt tatcccgggc ttaaccgcac tatgctgaca cgctcacggc tcactccgtc
     2821 acggcttatc cggggataac aaaggaaatt atattatggc aagacgaacg aagcgtagcg
     2881 cagttagggc gcaatcttat gacaggacga acgaagtagg gcttctcgtc ttatggcaag
     2941 acgaaggagc gcagcgacta gggcttcctt tatgacaaca gactgcctgt tttgcaaaat
     3001 ggtgtcagga cagatcaagc cgaacacggt gtacgaggac ggcgacgtgc tggcgttcaa
     3061 ggacatcaac ccgcaggccc cggtgcacgt tctggtgatt cccaaaaagc acattgccac
     3121 gctgaacgac ctcgacaccg gccacatcga gctgaccggc aagctgatca gggtggccgc
     3181 gcgcatcgcc agggaatctg ggatcgccga atcgggttac cgcatcgtca tgaactgcaa
     3241 cgccgacgcc ggccagtcgg tgtttcatgt ccatctgcat ctgctcggcg gccgccccat
     3301 gcattggccg ccgggttagc ggaaataaaa agggcgtgaa gaaaatcctt cacgccccgg
     3361 gagtcccgct ttaattcaga acggaatgtc gtcgtcgaaa tccccggcca cggccgtgtt
     3421 gccggccttc ttggctcccg ccggaccgct gttctccgct gcggacggct gatcctggtt
     3481 gaaattgtcg ctcggaacac cggcgccgcc gcgcgaaccc agcatctgca tttcgttcgc
     3541 gacgatctcg gtggtatagc gctcgtggcc ttccttgtcc tgccacttgc gcgtctgcaa
     3601 ccgcccttcc acgtacacct gcgagccctt cttcaggtat tcgccggcga tctccgccag
     3661 gcgcccgaaa aacaccaccc ggtgccattc ggttttctcc tgcttctcgc cggaattctt
     3721 gtccttccac gattccgagg tggccagcgt gacgttcgcc accgccgagc cgttggggga
     3781 ataacgcacc tcggggtcgc gccccagatt accgatcaga attaccttgt ttacaccgcg
     3841 tgccatgctc gattctccat tggttaatca cggagccctg tgttaatggt tgaaaaaccc
     3901 tttgtatcct caggttcgca accggttcat ctctacttca ctcttctccg tgaaagttaa
     3961 gggcgatgca tcatagccag attcatgcga acgctcaacg tggacgacaa acccgcgccg
     4021 gttcatcgat ttagaaggag ccctaaaatg cctgtctcaa gcgaagacgc gaagtccgcg
     4081 aagtattttc tgattgattc atgcgccttc acgtgcaaaa aaatctataa atcaaaactt
     4141 ccttaaggca acaggcgccg cagcgccggc tcatcaaacc ggcgactgtc caccttgaga
     4201 taggccacgc cctcgtccgc catcacggtg gcttcggtca cgccctgcac gcgggtgagc
     4261 tggttttcca ggcggcgggc atcctccgtg ttgagggtgt tgatgtgaaa catcaggttt
     4321 ttcaccgcgg gcggtgcctt cattccatac gccagataca gccatatgcc gatcaggatc
     4381 gccccgaact cgaatacggc ggcggcgccg aaatgatctg aaagtacccc acccatgaca
     4441 ccgccgacga aaagtcccag cgcctgcgag gtgttataga cacccaaagc cgtgcccttg
     4501 gcccggaccg gggcgatttt gctcaccagc gagggcaggc ttgcctccag cacgttgaag
     4561 gccgtgaaat acaacgtcag caccgtgacc agaccccaga aatgctggag ggcgggaaca
     4621 aaagcaaact ccacggccag catcagcgcg atggaaccga cgaaaaccgg tttggcctgc
     4681 gccttctttt cactgtagat aatcagcggc accatgaata cgacggaaat cgccagcacc
     4741 ggcagataca cctgccagtg atggttggca tcgaggccgc cggtttcgcg cagcgcgaac
     4801 ggcaccacca cgaacatcgc catctgcacc acgtgcagtg cgaaaatgcc gaaattcagc
     4861 cgtgcgagct cgcgatcacg caacacgccg aggagtccgg cgggcgtggc ctcggcatcg
     4921 gaatggaaat gactttgccg tggatcgggc acgatgaatt tcatcatcag gatcgccagc
     4981 atggccagca cgcccgtcat ggcgaaaatg cccggaatgc tgatgaggtg atatagcgcc
     5041 ggtcccagaa taagggaaag cgcgaaggtc aacccgatgg tgccgccgac catggccatg
     5101 gccttggtgc ggtgttcctc gcgcgtgagg tccgccagca gcgccgtcac tgccgcgttg
     5161 atcgcgcccg cgccctggat cgtgcgaccg ataataacca tgtagatatc gtcggccgcg
     5221 gcagcgataa aactccccag cgcgaacaac accaagccga aataaataac acgtttccgg
     5281 cccaggcggt ccgaggccat gccgaagggc aattgcagca atgcctgggt cagcccgtaa
     5341 acacccagcg ccagtccgat gagagagtga ttttcaccac ccggcagatg ctccgcgtaa
     5401 accgcaaaca ctggcaggat caggaacatc cccaacatgc gcaggccata aatactggcc
     5461 agccctgcgc tggcgcgccg ctccagcggt gacatacggt cttgcgggga agacattggt
     5521 tggattggga aaaattggct cttttgcgta tattaacaga ttcgcctttg acccggcatc
     5581 tgtcgcagac acacagcagc atggaaacca tccgtatccg cggtgcgcgc acgcacaacc
     5641 tcaagaacat cgacgtcgac ctcccgcgcg acaagctcac cgtgatcacc gggctgtccg
     5701 gctccggcaa gtcttcgctc gcctttgaca cgctgtacgc cgagggtcag cgccgttacg
     5761 tcgagtcgtt gtcggcctac gcgcgccagt ttctggcgct catggaaaaa ccggacgtgg
     5821 acctgatcga aggcctgagc ccggcgatct ccatcgaaca gaagtccacc tcgcacaatc
     5881 cgcgttccac ggtcggcacg gtcacggaaa tctacgacta cctgcgcctg ctgtatgcgc
     5941 gcgtgggcga gccgcgctgc ccggaacacg gcgtgacgct ctcggcccag accgtgagcc
     6001 agatggtgga tcacgtcatg acgctgcccg agggcaccaa gctcatgctg ctggcgcccc
     6061 tgatcgagga acgcaagggt gagcacctcc acatcctgga acgattacgt acccaaggct
     6121 atatccgcgc gcgcatcgac ggcacggtgg tttcgctcga tgaagcaccg gagctcaaga
     6181 agaatttcaa acacacgatc gaggcggtgg tggaccgcgc ggtggtgcgc ccgggacagg
     6241 agcagcgcct ggccgagtcc ttcgaaaccg cggccaacct gggagagggt ttgatccgtg
     6301 tggtggtgct ggacgaacag aacaacagca cgcaggaaat cgttttttcc gcgcgctacg
     6361 cctgcccgca ttgcggatac agtctggctg aacttgaacc gcggctgttc tccttcaaca
     6421 accccgccgg cgcctgcccc gagtgcgacg gtctcggcgt gcgccagttc ttcgatcccg
     6481 cgcgcgtggt agccgacccc accctgtcat tgcccgccgg cgcgatacgc ggctgggacc
     6541 ggcgcaacgc gttttatttc cacatgctgg aatcgctggc gaaacattac aagttcgacc
     6601 ttgatgcgcc gttcgagaaa ctcccaaaaa aggtgcgcga cgtgatcctg cacggcagcg
     6661 gcgacgaaat cattagcttc acttatttcg ccgagcaggg accgcgcgcc aaacgcaaac
     6721 acgccttcga gggcgtgatt cccaacatcg agcggcgcta ccgcgagtcc gaatccctgt
     6781 ccgtgcgcga ggaactggcg aaattcctca gccaccagcc ttgcgaaacc tgcaacggct
     6841 cgcgcctgaa acgcgaggcc cgacatgtgt tcgtggaagg caaggcgctg catgaaatca
     6901 cccaattgcc cgtggcgaac gcgctcgact ttttccaggg cctgcgtctg accgtaagcc
     6961 gcggcgaaat cgccgccaag atcgtgaaag aggtcgccaa ccgtctcg
//
LOCUS       DBNGZE010000374         3034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_122250, whole genome shotgun sequence.
ACCESSION   DBNGZE010000374 DBNGZE010000000
VERSION     DBNGZE010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_122250"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1671
                     /gene="pepP"
                     /locus_tag="AC16FV_09580"
     CDS             370..1671
                     /gene="pepP"
                     /locus_tag="AC16FV_09580"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HIR2850340.1"
                     /translation="MDMKIFEKRRETLMSHMGGGVAILPTAPMRVRNRDIHYPYRADS
                     DFYYLTHFPEPEAVLVLVPGREHGEYILFCRERNPEKEIWEGRRAGLEGARDIYGADD
                     AFPIDDIDEILPGLLENRDKVFYSMGRYPDFDAHLMNWVNEVRAKSRNGVHAPGEFVT
                     LDHILHEMRLFKGPEEIRLMKRAAKISAQAHRRAMQTCRPGMMEYEIEAELLHEFRKG
                     GSPFPAYPPIVGGGANGCILHYTENNAELKPGDLLLIDAGAEIDGYAADVTRTFPVNG
                     RYSGEQRAVYELVLAAQKAAIEQVRPGKHWNDPHENAVRVLTQGLKDLGLLNGAVDGH
                     IENGDYKRFYMHRTGHWLGMDVHDVGDYKLDDTWRLLEPGMVLTVEPGLYIAAAKDIP
                     EGFRNIGVRIEDDVLVTRDGNEVLSRDAPKAVDEIEVLMKK"
     gene            1757..2977
                     /gene="ubiH"
                     /locus_tag="AC16FV_09585"
                     /gene_synonym="visB"
     CDS             1757..2977
                     /gene="ubiH"
                     /locus_tag="AC16FV_09585"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HIR2850341.1"
                     /translation="MKIDYDILIIGGGLVGASLACALRASPLRIGVIEAVPLAASTQP
                     SYDDRTLALAWGSKRVFESMGCWQDIAPEATPIERIHISDRGRFGVTRLAASEAGLPA
                     LGYVVPNRALGVVLLKASQASKNIEWLCPAEMQEIRLNPEAASVTVRHDNASKTLTAR
                     LVIAADGAHSAVRAALGIEAERTEYCQSAVVTTVTASEPHGNTAYERFTDTGPLALLP
                     LRKNECAVVWSAKEAEVPTILGWSDAEFLNRLQDRFGDRLGTFTRPGKRAAYPLALTR
                     VKEHVRERLALIGNAAHTVHPVAGQGFNLGLRDVASIAEILTDAVRVSEDIGGLTVLR
                     RYADWRRRDNQITAGFTNGLIRIFSNNAFPLTFLRNAGLLAVDLMPGVKRGFVRVTSG
                     LAGKLPRLARGLPL"
BASE COUNT          590 a          942 c          971 g          531 t
ORIGIN      
        1 gcggttcaat gcgctgcgca cgcgctcgcg gtcttgctgc acctgggccg cgttgatgac
       61 cgcctcggtg ccgatcatgc cggcatgcgc cgcgggcaga ggcaggctca gcatgagcaa
      121 gccgaagatg accagatggc tgacgggttt ggcgagaaga cgaagttttt ccattgttat
      181 gtctcctacg gagtggaatg aagatggtac agcataattt gaccggaccg aaccggggta
      241 acggccctgt ttggtcttgt gaaggatacc ggttgtcgga tatacaccct attgtgtggg
      301 gattataaca agccgccgcc tgaccaccag cggccgacgc accgccgccg attttggcat
      361 aatgccttta tggatatgaa aattttcgaa aagcgccgcg agacgctgat gagtcacatg
      421 ggcggcggcg tggccatcct gccgacggcg ccgatgcgcg tgcgcaaccg cgacatacat
      481 tatccgtatc gcgctgacag cgatttctat tatctcaccc attttcccga acccgaggcg
      541 gtgctggtgc tggtgccagg gcgcgaacac ggcgaataca tcctgttctg ccgcgagcgc
      601 aacccggaaa aggaaatctg ggaaggccgg cgcgccggcc tcgagggcgc gcgcgacatc
      661 tacggcgccg acgacgcctt tcccatcgac gacattgacg agatcctgcc gggcctgctc
      721 gagaaccgcg acaaggtgtt ctacagcatg gggcgctacc cggatttcga cgcgcacttg
      781 atgaactggg tcaacgaagt ccgcgccaag agccgcaacg gcgtgcacgc acccggagag
      841 ttcgtcaccc tcgatcacat cctgcacgag atgcgcctgt tcaaggggcc ggaggagatc
      901 cggctgatga aacgcgccgc gaaaatttcg gcacaggcgc accggcgcgc gatgcagacc
      961 tgccggccgg gcatgatgga atacgagatc gaggccgagc tgctgcacga gttccgcaaa
     1021 ggcggcagtc cgttcccggc ctatccgccg atcgtcggcg gtggcgccaa cggttgcatc
     1081 ctgcattaca ccgaaaacaa cgccgagctg aagcccggcg acctgctgct gatcgacgcc
     1141 ggcgccgaga tcgacggcta cgccgccgac gtcacgcgca ccttccccgt caacggccgc
     1201 tacagcggcg agcagcgcgc ggtctacgag ctggtgctgg cggcgcagaa ggcggcgatc
     1261 gagcaggtca ggcccggcaa gcactggaac gacccgcacg agaacgcggt gcgcgttctg
     1321 actcaaggcc tcaaggacct tgggttgctg aacggtgcag tggacggaca catcgagaac
     1381 ggcgattaca agcgtttcta catgcaccgc accggccact ggctcggcat ggacgtgcac
     1441 gacgtcggcg actacaagct ggacgacacc tggcgcctgc tcgaacccgg catggtgctc
     1501 accgtcgagc ccggcctgta catcgccgcg gccaaggaca ttccggaagg tttccgcaac
     1561 atcggcgtgc gcatcgagga cgacgtgctg gtcacgcgcg acggcaacga ggtgttgtcg
     1621 cgcgacgcgc ccaaagccgt tgatgaaatt gaagtattaa tgaagaaata gttttcaccg
     1681 ccagggtcgc caggaaaaac ggttcttgaa atctaaatcc ttggcgttcc tggcgccctg
     1741 gcggtttaaa aatcatatga aaattgatta cgacattttg atcatcggcg gcgggctggt
     1801 cggcgccagt cttgcctgtg ccttgcgcgc gagccccctg cgcatcggtg tgatcgaagc
     1861 cgtgccgctc gcggcctcca cccagccgag ctatgacgac cgcacgctgg cgctggcttg
     1921 gggctcgaaa cgggtatttg aaagcatggg ttgctggcag gacatcgcgc cggaggccac
     1981 ccctatcgag cgcatccaca tctccgaccg cggccgattc ggcgtcacgc ggctggcggc
     2041 cagcgaggcc ggcctgccgg cattgggcta tgtcgtgccc aatcgggcac tcggggttgt
     2101 gctgctgaag gcctcgcagg catcgaaaaa catcgaatgg ctgtgcccgg cggaaatgca
     2161 ggaaatcagg ctcaaccccg aagccgcgtc ggtaacggtg cgtcatgaca acgccagcaa
     2221 gacgctcacg gcacgtcttg tcatcgccgc cgatggcgcg cattccgcgg tgcgcgcggc
     2281 cttggggata gaggcggaac gtactgagta ctgccagagc gccgtcgtga ccacggtcac
     2341 ggcgagcgaa ccccatggca acaccgccta tgagcgcttc acggataccg gtccgctggc
     2401 cctgctgccc ctgcgcaaaa atgaatgcgc ggtagtgtgg agcgcgaaag aggccgaggt
     2461 gccgactatc ctcggctgga gcgatgcgga attcctgaat cgtttgcagg accgcttcgg
     2521 tgaccggctc gggactttca cgcggccggg caagcgtgcc gcctatccgc tcgcgctcac
     2581 gcgggtgaaa gaacacgtgc gcgagcgcct cgcgctcatc ggcaacgccg cgcacacggt
     2641 gcatccggtc gcgggacagg gtttcaatct cggtctgcgt gacgtggctt ccatcgcgga
     2701 gattctcacg gacgccgtgc gcgtcagcga ggacatcggc ggcctcacgg tgctgcgccg
     2761 ctatgccgac tggcgccggc gcgacaacca gatcacggcc ggcttcacca acgggctcat
     2821 ccggattttt tccaacaacg cctttccgct gacgttcttg cgcaacgccg ggctgctggc
     2881 ggtggacctg atgccgggcg tgaagcgcgg ttttgtgcgt gtcaccagcg gcctggccgg
     2941 aaaactcccg cgcctcgcgc gcgggttgcc gctgtaaact aacaagggtt cgcaactgga
     3001 aacagactgt cctttaactg gcggctatga tgtc
//
LOCUS       DBNGZE010000375         1812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_609309, whole genome shotgun sequence.
ACCESSION   DBNGZE010000375 DBNGZE010000000
VERSION     DBNGZE010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_609309"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC16FV_09590"
     CDS             complement(<1..479)
                     /locus_tag="AC16FV_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR2850342.1"
                     /translation="MRFTPQFLDELKARLPVSEVVGKRVKLLRAGREFKGLSPFNKEK
                     TPSFFVNDQKQAWFDFSSGKNGSIFDFVMQSEGVSFPEAVERLAQMAGVPLPKISRED
                     EARDVRRKTLHDVMELAAKFFQDTLASRAGAKARGYLADRGLDPATQLKFRIGYAPG"
     gene            600..752
                     /locus_tag="AC16FV_09595"
     CDS             600..752
                     /locus_tag="AC16FV_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850343.1"
                     /translation="MAGIGTANPCFSCTKSQGRQSYRRTYEVPYGHAMMVDEPNRLTE
                     ILLEVA"
     gene            complement(766..1791)
                     /locus_tag="AC16FV_09600"
     CDS             complement(766..1791)
                     /locus_tag="AC16FV_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR2850344.1"
                     /translation="MPLRTCNGQLQPGDVFGERVMQRFAMYFALTAGLMASATAVRAD
                     DYPSRPIHVLTTSSAGGISDIFMRVLGEELHKSLGQPLIIENRPGGAGNIGARACQDA
                     APDGYTICIINADPMIYNQYLFKSLPFDPDKLTPIINLFHLIQVLVINSDLGAKTVDE
                     LVAVSKQKPGTLSYLTASIPCVVYMDSLKQDKGSDWVRVPFKGGGEATNAILGGSTPI
                     GLIGLGNVIAHIRAGKMNALALVNNIRTPLLPDVPTLSDLGYKGAPSQTWYGLFAPPG
                     TPKPIVDKIAAEVTRVVSDAGFRDKYIISRGQVPAINTPEQFAAEIKADRAAAQQVVK
                     ASGLQPQ"
BASE COUNT          352 a          562 c          537 g          361 t
ORIGIN      
        1 tcgcccggcg catatccaat tcgaaatttg agttgcgtcg ccggatcgag cccgcgatcg
       61 gcgagatagc cgcgcgcttt cgcaccagcg cgcgaagcca gcgtgtcctg gaaaaatttc
      121 gccgccagtt ccatgacatc gtgcagcgtc ttgcgccgca catcgcgcgc ctcatcctcg
      181 cgcgagatct tcggcaacgg cacgccggcc atttgcgcga gccgctccac cgcttccgga
      241 aagctaacgc cttcgctttg catgacgaaa tcgaagatgc tgccgttctt gcctgaggaa
      301 aaatcgaacc acgcctgctt ctggtcgttc acaaagaatg acggcgtttt ctccttattg
      361 aacggcgaca gacccttgaa ctcacgtccg gcgcgcaaga gcttgacgcg cttgcccacg
      421 acctccgaga ccggaagccg ggctttcagc tcatcgagga actgcggtgt aaagcgcatg
      481 ggttttgggg ccgaatcggc ggctaaccgc caactcttcc ccatatcggg tatttacggc
      541 gcgtccaggg ataatttgtc cccgctagtc acaggagcac ctcacgcgga aaccctataa
      601 tggccggaat aggcacggcc aatccgtgtt tcagctgtac aaaaagccaa ggcagacaaa
      661 gctatcggcg cacttatgaa gtgccatacg gccatgccat gatggtcgac gagcctaacc
      721 ggctgacgga aatcctgctc gaggtcgcgt gaccggttgc ttaggctact gcggctgcaa
      781 ccctgacgcc ttcaccacct gctgcgctgc cgcgcggtca gccttgatct cagcggcgaa
      841 ctgctccggc gtgttgatcg ccggcacctg gccgcggctg atgatgtatt tgtcgcggaa
      901 gccggcatcg ctgaccaccc gcgtgacttc ggctgcgatc ttgtcgacga tcggcttcgg
      961 cgtgccgggc ggtgcgaaca gcccatacca ggtctgcgac ggcgcgccct tgtagccaag
     1021 atcactcagc gtcggcacgt ccggcaacag cggggtgcga atgttattca ccagcgcgag
     1081 cgcgttcatc ttgccggcgc ggatatgcgc gatcacgttg ccgagcccga tcaggccgat
     1141 cggcgtcgag ccgccaagaa tggcgttggt cgcctcgccg ccgcccttga atggcacgcg
     1201 cacccagtcg ctgcctttgt cctgcttgag gctgtccata tagacaacgc acggaatcga
     1261 ggcggtgaga taagagagcg tccccggctt ctgcttcgac actgccacca attcgtccac
     1321 agttttggcc ccgaggtcgg aattgatcac cagcacctgg attaggtgga agaggttgat
     1381 gatcggtgtg agcttgtcgg ggtcgaaggg caagctcttg aacaggtatt ggttgtagat
     1441 catcggatct gcattgatga tgcagatcgt gtaaccgtcg ggcgccgcat cctggcaggc
     1501 gcgtgcgcca atattgccgg cgccgccggg ccgattttcg atgatcagag gctggccgag
     1561 gctcttgtgc agctcctcgc ctagaacgcg catgaagatg tcgctgatac cgcccgcgct
     1621 tgaggtggta agcacgtgga tcgggcggct tggatagtca tccgcccgca ccgccgttgc
     1681 ggatgccatc aggccggccg tcaacgcaaa atacatcgca aagcgttgca ttaccctctc
     1741 cccaaagaca tcgccgggct gcagttgacc attgcaggtg cgcagcggca aggcagtcgt
     1801 accaccgcat ca
//
LOCUS       DBNGZE010000376         2436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1090048, whole genome shotgun sequence.
ACCESSION   DBNGZE010000376 DBNGZE010000000
VERSION     DBNGZE010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1090048"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="AC16FV_09605"
     CDS             complement(<1..237)
                     /locus_tag="AC16FV_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR2850345.1"
                     /translation="MTVFSTIHELLADKPANAIAFVGSGCPSLTYGGLRDHVQRTVAS
                     LNRLGIGRNDRVAIVLPNGTEMASAFVAIAAGMTS"
     gene            complement(511..933)
                     /gene="mscL"
                     /locus_tag="AC16FV_09610"
     CDS             complement(511..933)
                     /gene="mscL"
                     /locus_tag="AC16FV_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR2850346.1"
                     /translation="MAIPQMHIPGKPLALLEEFKNFALKGNVIDLAVGVIIGAAFGKI
                     VDSLVKHLIMPTIGVMLPGEQGYLGWKLVINGKDIPYGLFLGEVVNFLIVALALYLFI
                     VKFLGFVMKAKKAEAAAPPAPTRDQELLTEIRDLLRKK"
     gene            complement(1202..>2436)
                     /locus_tag="AC16FV_09615"
     CDS             complement(1202..>2436)
                     /locus_tag="AC16FV_09615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2850347.1"
                     /translation="IDLDHFKTINDALGHDVGDEALREVARRLTNIVAGYASLDIFLV
                     RLGGDEFVIVISGISRDKEEATSVSRELAERVIENMLRPLVVGERVLNVGASIGVALF
                     ADEGMTESDILRHADMALYRAKNSGRNTIQFYEPPMQAAADERHALEKGIRAALDNGE
                     LELHFQPQVDGMGRMIGAETLLRWHHSERGDIPPATFIPVAEETGLIHSIGAWALNQA
                     CERLRAWLRAGIPFTGHLSINVSQWQLVRPDYVQQLSDVLAKCDMEPGRLILEVTESS
                     LLYSPEETIEKLGALRAMGLKIALDDFGTGYSSLAHLKDLPLDLIKIDKAFVNELTRA
                     SEHPLVESIIAIGRNMKLNIIAEGVETEIQRYILVQMGCNGFQGHLIRQPLPEKDFVA
                     WMTENHAASNAPVLQQRQ"
BASE COUNT          492 a          713 c          701 g          530 t
ORIGIN      
        1 cgaggtcatg cccgcggcga tggccacgaa ggcgctggcc atctcggtgc cgttgggtag
       61 cacgatggcg acgcggtcgt tgcgcccgat cccgagccgg ttcaggctcg ccacggtgcg
      121 ttgcacatgg tcgcgcaggc ccccataagt gagcgacgga cagccgctgc cgacaaaggc
      181 aatggcgttg gcgggtttat ccgccagcag ctcatggatt gtagaaaaga cggtcatatg
      241 ttttccttaa aaacgccgtt cgccctgagc ttgtcgaagg gtgaacggga aatgcggtaa
      301 atgtcaggag ttcgtccgtt catggttcga caggctcacc acgaacggac ttaatcagac
      361 cttccctcac gaaattattc aggacagcaa tgtcccgaat gcgccgtcat gttatcagcc
      421 tttcgcaggc tgtgaatccg tttttcatga tgcgcaacaa cgacccgccg gggaaactcg
      481 gttacgtccg tttgccgatc gtcccggcgc tcactttttt ctcagcaaat cgcgaatttc
      541 ggtcagcagt tcctgatccc tggtcggcgc gggcggggcg gcggcctcgg ccttttttgc
      601 tttcatgacg aagccgagga atttgacgat gaacagatac agcgccagcg ccacgatcag
      661 gaagttgacc acctcgccga gaaaaagccc gtaagggatg tccttgccgt tgatcaccag
      721 cttccagcca agataaccct gctcacccgg cagcatcacg ccgatcgtgg gcatgatgag
      781 gtgtttcacg agcgagtcga cgatctttcc gaaggcggcg ccgatgatga cgccgacggc
      841 gagatcgatg acattgccct tgagcgcgaa gttcttgaac tcttccagca gcgccagtgg
      901 ttttccggga atatgcatct ggggaatggc catacaacct cctttcaggt ttttgctgtt
      961 ggtttttcag gaccaagcgg cgatgagcga tttcccaccg ccatgccggg accgacggtc
     1021 cagctgaatc ataccccggg ccttcggtaa tttattgatg aatggcgaaa aaaaacttcc
     1081 ttgccggaga aggtggaaga cagccgccag atcatcttgc agaccgggct cgttgcgcgt
     1141 tatggcgccg cgctgatcct gtcctcggga tccgacgtcc gatcgatcta acgcaacaga
     1201 tttattgacg ctgttgtaaa accggagcgt ttgacgcggc gtgattttcc gtcatccatg
     1261 ccacgaagtc tttctcgggc agcggctggc ggatcaggtg gccctgaaaa ccgttacaac
     1321 ccatttggac cagaatataa cgttgtattt cggtttcgac gccttcggcg atgatgttca
     1381 atttcatgtt ccgcccgatg gctatgatgg attccaccag cggatgttcc gaggcccggg
     1441 tgagctcgtt gacgaaggcc ttgtctattt tgatcaagtc cagcggcagg tccttgagat
     1501 gcgccaggga cgagtagccg gtgccgaaat cgtccagcgc gatcttgagc cccatagcgc
     1561 gaagcgcccc cagtttttcg attgtctcct cgggggaata taacaaggag ctttcggtaa
     1621 cttccagaat cagccgcccg ggctccatgt cgcatttggc cagcacatcg ctgagctgct
     1681 ggacgtaatc aggacgaacg agttgccact ggctgacgtt gatcgacaga tgtccggtaa
     1741 acggaatccc ggcccgcagc cacgccctga gccgctcgca ggcctggttg agggcccatg
     1801 ccccgatgga atgaatcaaa ccagtttcct cggcgaccgg gatgaacgtg gccggaggaa
     1861 tgtcgccccg ttccgaatga tgccaccgaa gcagtgtctc ggccccgatc atccgcccca
     1921 tgccgtcgac ctgcggctgg aagtgcagtt ccagttcgcc gttatccagc gccgcgcgaa
     1981 ttcccttttc cagcgcgtgt cgctcatcgg ccgccgcctg catgggcggt tcgtagaatt
     2041 gaatggtgtt gcgcccgctg ttcttggccc gatacaaggc catgtcggcg tgtcgcagaa
     2101 tatccgattc ggtcattcct tcgtccgcga acagagcgac gccaatgctg gcgccgacat
     2161 tgaggacgcg ttcacccacg acaagcggac gcagcatatt ttctatcacc cgttccgcca
     2221 gctcgcggga taccgacgtc gcttcttcct tgtcccggga tatcccggat atgacgatca
     2281 caaactcatc gccgcccagg cgcacgagaa agatatcgag agaggcgtag ccggcgacga
     2341 tgttcgtcag gcggcgggcg acttcgcgca acgcctcatc gcccacgtcg tggccgagcg
     2401 cgtcgttgat cgttttgaag tgatccagat cgatca
//
LOCUS       DBNGZE010000377         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_540419, whole genome shotgun sequence.
ACCESSION   DBNGZE010000377 DBNGZE010000000
VERSION     DBNGZE010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_540419"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="AC16FV_09620"
     CDS             <1..533
                     /locus_tag="AC16FV_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR2850348.1"
                     /translation="FMYVNSGGGTAANLSEFDVYRFPLTGYSAANPPNTPARTLLYSD
                     DVTPDRDSHGMVLSKHGRYVWVADRDLDVIEVFDTATGNRVNTISLQGITANLSLDLG
                     DITPSGHRVYFSTSGPNPLSGDPHASTGDLPGLVAIQVEQGGKHGVFKGNVRISNVDA
                     GGIERADGHGLRVRRK"
     gene            670..855
                     /locus_tag="AC16FV_09625"
     CDS             670..855
                     /locus_tag="AC16FV_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850349.1"
                     /translation="MTDNENSGTPPDNRERRKKMRRLGSDRRGQPRWDDNSPIRRKNP
                     GRRALDRLLSALDLLKK"
     gene            1028..1255
                     /locus_tag="AC16FV_09630"
     CDS             1028..1255
                     /locus_tag="AC16FV_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR2850350.1"
                     /translation="MADFELDMRGQTCPLPVMRTAKKMLTMKLGQTLRVLATDPTTVT
                     DFDAYVRGSGNTLLESKKTAEGEFEFLLKRA"
     gene            1318..1791
                     /locus_tag="AC16FV_09635"
     CDS             1318..1791
                     /locus_tag="AC16FV_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850351.1"
                     /translation="MQHIKIAAIGAAFALTLLTAQSATAGPYSDDMAKCLVTSTSEAD
                     KNSLVKWMFAAATLHPAVKSISSVTDAQRNELNVKTAKLFERLLTESCKSQVQTAIKY
                     EGQSTIESAFQVLGQVAARGLFSDPSVNGFIAQLGNHIDKHKLESVLNIASKKPN"
     gene            complement(1997..2407)
                     /locus_tag="AC16FV_09640"
     CDS             complement(1997..2407)
                     /locus_tag="AC16FV_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2850352.1"
                     /translation="MNMNTAFFNSWQPRALAVLRIVTAYLFIAHGTAKLFGVPHVAMF
                     DGLQLMSLVGLAGVLEAGGGALLLIGLFTRPVAFVLCGFMAVAYFMAHANQGNVLLPM
                     LNGGELAVLYSFVFLYFVFAGAGAWSVDAARKRR"
BASE COUNT          587 a          824 c          719 g          506 t
ORIGIN      
        1 ggtttatgta cgtgaactcg ggcggcggca cggcggcgaa tctctccgag ttcgacgtct
       61 atcgcttccc gctgaccggc tacagcgccg ccaacccgcc gaacacgccg gcacggacgc
      121 tgctgtactc cgatgacgtg acgccggacc gcgactcgca cggcatggtg ctctccaagc
      181 acggtcgcta tgtctgggtc gcggaccgtg acctcgacgt gatcgaagtg ttcgacaccg
      241 ccaccggcaa tcgcgtgaac accatcagcc tgcaaggcat caccgcgaac ctgtcgctgg
      301 acctgggtga catcacgccc tccggccacc gggtgtattt ctcgacgagc gggccgaacc
      361 cgctttccgg cgacccccac gcctcgaccg gcgacctgcc gggcctggtc gcgatccagg
      421 tcgagcaagg cggcaagcac ggcgtattca agggcaacgt gcgcatcagc aacgtggatg
      481 ccggcggcat cgagcgcgcc gacggccatg ggttgcgtgt gcggcgcaag taaatagccc
      541 gacccgctga gaacaaccaa gcctccccct ctcccgccgg gagaggggga taggcgctgc
      601 ctgcgtcttg gagggagtat cgtttttgag ctactctcta agcagggatc gacgcggcaa
      661 gggaattcca tgaccgacaa cgaaaactcc ggcactcctc ccgataaccg cgagcgccgc
      721 aagaagatgc ggcgccttgg cagcgatcgt cgtggtcaac cgcgctggga cgataacagc
      781 cccatccgtc gcaaaaatcc cggccgtcga gccctcgacc gcctgctcag cgcgctcgac
      841 ctgctcaaga aatagccggc tgcaccgccc gccggacggg ccggcgggaa tttcgcccga
      901 tattagtcca tacgtatcag ggacattaca gacgtcaaga acatccccct cctgccctgc
      961 aaaaatgttt taacgatcta tagtttcagg atgtgccctg attttgccgt taatccggag
     1021 agtatttatg gctgattttg aactggacat gcgtgggcag acttgcccgc tgcctgtcat
     1081 gcgtaccgcg aaaaaaatgc tgaccatgaa gcttggccag acgctgcgtg ttttggcaac
     1141 cgatcccact actgttacgg atttcgatgc ctacgtaaga ggctcgggta acaccctgct
     1201 cgaatccaaa aagaccgccg agggtgagtt cgagtttttg ctgaaacggg cgtagtcatt
     1261 tccaaaacgg tgtcagtgat cgcgctgaca cgtccaacac ctgaaaggga gcttctcatg
     1321 caacatatta aaatcgctgc catcggcgcc gcgttcgcgc tcaccctcct gaccgcacaa
     1381 tccgcaaccg ccggtccgta ttccgacgat atggcgaagt gcctcgtcac ctcgaccagc
     1441 gaggccgaca agaattcact cgtgaagtgg atgttcgccg ccgcgaccct gcatcccgcc
     1501 gtcaaatcga tcagttccgt taccgatgcg cagcgtaatg agctgaacgt gaaaacggcg
     1561 aagctatttg aaaggctgct caccgaatcc tgcaaatcgc aggtgcaaac ggcgattaaa
     1621 tatgaaggtc agagcaccat cgaatccgcc tttcaggtgc tcggccaggt cgccgcacgc
     1681 gggttgtttt ccgacccgag cgtcaacggc ttcatcgccc agctggggaa tcacatcgac
     1741 aagcacaaac tggaaagcgt cctgaatatt gcgtccaaga aaccaaatta aggaacctct
     1801 gattaattcc gttcgtggtg agcttgtcga accatgaacg ggcggaaacg taatccgttc
     1861 aatcgctttc cgttcgccct tcgacaggct cagggcgaac gggcgaataa atcagaggtt
     1921 ccttaaccgc gttgtttcag tcctgatgta gataaacgaa agccgcccat cgggcggctt
     1981 ttgtcttcgg tgacgctcac cgccgtttgc gggccgcgtc cacgctccag gccccggcgc
     2041 ccgcaaagac gaaatacagg aacacgaagc tgtaaagcac cgcgagttct cccccgttca
     2101 acatcggcaa cagcacattc ccctggttgg catgcgccat gaagtacgcc acggccatga
     2161 atccgcacag gacgaacgcg accggccgcg tgaacagccc gatgagcagc agcgcaccgc
     2221 cgccggcctc caggaccccg gcaagcccga ccagcgacat caactgcaac ccatcaaaca
     2281 tcgccacgtg cggcacgccg aacagcttgg ccgtgccgtg cgcgataaac agatacgccg
     2341 tgacgatgcg caagaccgcc agcgcgcgcg gctgccacga attgaagaat gccgtattca
     2401 tattcatagt cccttgttgg ttgagtcatg atcttgcgcg aaaaatggat gattcgccaa
     2461 cggtggagat tcccatccgt tcctacggga cttgcataag cgctcctgag ccaacgcctt
     2521 aatatttcgc cagttgcatt gtttttaccg ggcattagca cggtttcgtc acgacctgac
     2581 tgatttggca cgttttctgc acaaatatcc agttggataa ccgttggcca cagatt
//
LOCUS       DBNGZE010000378         3665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_956190, whole genome shotgun sequence.
ACCESSION   DBNGZE010000378 DBNGZE010000000
VERSION     DBNGZE010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_956190"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /gene="hflK"
                     /locus_tag="AC16FV_09645"
     CDS             complement(<1..1068)
                     /gene="hflK"
                     /locus_tag="AC16FV_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR2850353.1"
                     /translation="MAWNEPGKPGDKDPWGQRRRPSSGGPDIDRIVKNIQQKFAGLFG
                     GGAGGGTHGSGFGIGLIIVVALAIWLLTGFYIVNQGERGVVLRFGKKAEITDAGLRWH
                     LPFPLEKVEKVNVEKVSTIEIGYRSNPRAGGKSKVPKEALMLTEDENIIDIEFAVQYK
                     IKDAAEYLFNVRDPETTIAQATESAVREVVGKSTLDFALTEGRDQVSRNSRELLQQIL
                     DRYKVGVHIVTVETQKAQPPEEVKSAFDDAVKAREDEQRLKNEAEAYANDVIPRARGA
                     AARMTQEAEGYKASVIARADGDARRFSLIANEYVKAPGVTRERLYLETMEQVLSNTTK
                     IFVDQKAGNNLIYLPLDKLVPM"
     gene            complement(1124..2443)
                     /gene="hflX"
                     /locus_tag="AC16FV_09650"
     CDS             complement(1124..2443)
                     /gene="hflX"
                     /locus_tag="AC16FV_09650"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIR2850354.1"
                     /translation="MNPASGARQRERALLVQLRLSGRPEPEALEELRLLAVSAGADVC
                     GAILGSRQIPDAATFVGKGKAEEIRDAVARERAELVLVNHDLSPAQERNLEKITSCRV
                     LDRTGLILDIFAQRAHSAEGKLQVELAQLEHLSTRLVRGWTHLGRQKGGIGLRGPGET
                     QLEVDRRLIRDRIKKLNERLEQVYVQRRSRRKARHRVPIPTVSLVGYTNAGKSSLFNR
                     LTSSAVYAADQLFATLDPTMRRIELPGKASVILGDTVGFIRQLPHDLVEAFKSTLEEV
                     AEADLLLHVVDAANPEKLEQIQQVNNVLTEIEAQSIPQITVFNKIDLTGEAPRFERDA
                     NGHISRLWVSARTGEGMELLLQALADHFRRHRKRYRFCLPPEAGRLRAVLYERFDVIR
                     ESVPETGGWMLELALEPAQFAWLRQQSDFRESYLVSEAESVLAPTGS"
     gene            complement(2451..2720)
                     /gene="hfq"
                     /locus_tag="AC16FV_09655"
     CDS             complement(2451..2720)
                     /gene="hfq"
                     /locus_tag="AC16FV_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008912237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR2850355.1"
                     /translation="MSQQKGQALQDPFLNVLRKEHVPVSIFLVNGIKLQGQIESFDQF
                     VVLLKNSVSQMIYKHAISTVVPSRPVKFHIEGMDSSDSSKGPGNE"
     gene            complement(2822..3655)
                     /gene="miaA"
                     /locus_tag="AC16FV_09660"
     CDS             complement(2822..3655)
                     /gene="miaA"
                     /locus_tag="AC16FV_09660"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR2850356.1"
                     /translation="MDSSQVYRGMDIGTAKPTPTEQARAPHRLIDVRDPTESYSAAEF
                     CADALREMEAITRAGKIPLLVGGTMFYFHALEFGLSDLPSADPEIRERLSAEAAQSGW
                     ETLHRRLQSIDPVSAARIHPNDAQRIQRALEVHELTGQSLSELAMARQRVSLPYHPIK
                     IALWPQDRKKLHAHIERRFHAMLEQGFLLEVEKLYARGDLQAAMPSVRTVGYRQIWEY
                     LTGKVNYSEMIKQALAATRQFAKRQITWLRSYPDIRAFDSSDSIPVRESQDYLREMIH
                     Q"
BASE COUNT          674 a         1122 c         1086 g          783 t
ORIGIN      
        1 catcggcacc agcttgtcga gcggcagata aatcaggttg ttgccggcct tctggtccac
       61 gaaaatcttg gtggtgttgg acagcacctg ttccatcgtc tccaggtaaa gacgctcgcg
      121 ggtcacaccg ggcgccttta catactcgtt ggcgatcagg ctgaagcggc gcgcatcgcc
      181 gtcggctcgc gcaatcacgc ttgccttgta gccttcggct tcttgggtca tgcgcgcggc
      241 cgcgccgcgg gcgcgcggaa tcacgtcgtt ggcataggcc tcggcctcgt ttttcaggcg
      301 ttgctcgtcc tcgcgcgcct tcacggcgtc atcgaacgcc gatttcacct cttccggcgg
      361 ctgcgccttc tgggtttcaa ccgtgacgat gtgcacgcca accttgtagc ggtccaggat
      421 ctgttgcagc agttcgcgcg agttgcgcga cacctggtcg cgcccttcag tcagcgcaaa
      481 atccagcgtg ctcttgccga ccacctcacg caccgcggat tcggtcgcct gggcgatggt
      541 ggtctcgggg tcgcgcacgt tgaaaagata ctccgcggca tccttgatct tgtattgcac
      601 cgcgaactcg atgtcgatga tgttctcgtc ctcggtcagc atcagcgctt ccttcggcac
      661 cttgctcttg ccgccggcgc gcgggttgct gcggtagccg atctcgatcg tggagacctt
      721 ctccacgttc accttctcga ccttctccag cgggaatggc aggtgccagc gcaggcccgc
      781 gtcggtgatc tcggccttct tgccgaaacg cagcacgacg ccacgctcgc cctggttgac
      841 gatgtagaaa ccggtaagga gccagatggc cagcgcgacc acgatgatca gaccgatgcc
      901 gaaaccgctg ccgtgcgtgc cgccgcccgc gccgccaccg aacagtccgg cgaatttctg
      961 ctggatgttt ttaacgatgc gatctatgtc gggaccaccg gaggacggac gccggcgctg
     1021 accccaagga tctttatccc cgggcttgcc gggttcattc catgccacgc tgtacccctt
     1081 ggtgatttag agggaaaact ccgcaaagag cggcatttta gggtcaggac ccggtgggag
     1141 caagaacgga ttcggcttct gaaaccagat aggactcgcg gaaatcactc tgttgccgca
     1201 accatgcgaa ctgggcgggc tccagcgcca actccagcat ccagccgccg gtctccggca
     1261 ccgactcgcg tatcacatcg aaacgctcat acaggacggc gcgcaaccgg ccggcctccg
     1321 gcggcaggca aaagcggtaa cgtttacggt ggcggcggaa gtgatccgcc agcgcttgca
     1381 gcaacagctc catgccctcg ccggtgcgcg ccgaaaccca gaggcgtgag atatgtccgt
     1441 tggcgtcgcg ctcgaagcgc ggcgcctcgc cggtcaggtc gatcttgttg aataccgtga
     1501 tctgcgggat ggactgcgcc tcgatctccg tcagcacgtt gttcacctgc tggatctgtt
     1561 cgagcttttc ggggttggcg gcatcgacga catgcagcag caaatccgcc tcggccacct
     1621 cttccagtgt cgacttgaat gcctccacca gatcatgggg gagttggcgg atgaagccca
     1681 cggtatcccc cagaatcacc gaagccttgc ccggcaactc gatacgccgc atcgtcgggt
     1741 cgagcgtggc gaacaattga tcggcggcat acaccgcgga cgaggtcaag cgattgaaca
     1801 acgaggactt gccggcgttg gtgtaaccga ccaacgacac ggtaggaatg ggcacccggt
     1861 ggcgcgcctt gcgccggcta cgacgctgga cgtacacctg ctcaaggcgt tcgttgagtt
     1921 tcttgatgcg gtcgcggatc aggcggcggt ccacttccag ctgtgtctcg ccgggaccgc
     1981 gcaggccgat accgcccttt tgccgtccca agtgggtcca gccgcgcacc aggcgcgtgg
     2041 acagatgttc gagttgggcc agttcgacct gcagcttgcc ttcggcggag tgcgcgcgct
     2101 gcgcgaagat atccaggatc aggccggtgc ggtccagcac gcgacaactc gtgatttttt
     2161 cgagattgcg ttcctgcgcg ggcgacaggt catgattgac gagcacgagt tcggcgcgct
     2221 cgcgcgccac ggcatcgcgg atttcctcgg ccttgccctt gccgacaaag gtcgcggcgt
     2281 ccggtatctg ccggctgccg agaatggcgc cgcagacgtc ggcgccggcg gacactgcca
     2341 gcagccgcaa ttcctcaagc gcctccggtt caggtcggcc cgacaggcgc aactgcacca
     2401 gcagggcgcg ctcacgctgg cgcgcccctg atgcaggatt cattgccgga ttattcgttg
     2461 ccagggccct tgctgctgtc ggaggaatcc atgccctcga tatggaactt cacggggcga
     2521 ctgggcacca ccgtggagat ggcatgtttg taaatcatct gacttacgga attctttagc
     2581 aataccacaa actgatcgaa agactcgatt tgtccctgca atttgatgcc gttgaccaga
     2641 aagatggata cggggacatg ttccttgcgc agaacgttaa ggaaagggtc ttgtaaggct
     2701 tgcccttttt gctgactcat gacagttctc ctgttcttat gttcgtagtg ccttgcggca
     2761 tttttggtcg tccggcctca ttcctgaccg gttagttata agtataacct cagaccggac
     2821 cctattgatg gatcatttcc ctcaaatagt cctgactttc acgcaccggg atcgaatcac
     2881 tgctgtcaaa ggcgcgtata tccgggtaac tgcgcagcca ggttatctgc cgcttagcaa
     2941 attgccgcgt tgccgccagc gcctgtttga tcatttcact atagttgact tttccagtca
     3001 agtattccca gatctgccga tagcctacgg tgcgcaccga aggcatcgca gcttggagat
     3061 cgccgcgcgc ataaagtttc tccacttcca acaaaaaacc ctgctccagc atggcatgga
     3121 aacgccgttc gatgtgtgcg tgcaatttct tacggtcttg cggccagagc gcgattttga
     3181 tcggatgata cggcagcgaa acccgctgcc gagccatggc caattcactt agggattgac
     3241 cggtaagttc atgcacttcg agcgcacgct ggatgcgctg cgcatcgttc ggatggatgc
     3301 gtgccgctga cacgggatcg atggactgaa gccgccgatg cagcgtctcc catcccgatt
     3361 gtgccgcttc agcactcagg cgctcgcgga tttccggatc ggcggacggc agatcggata
     3421 gtccgaattc cagtgcatga aaatagaaca tggtgccacc gacgagtagt ggaattttgc
     3481 cggcgcgtgt aattgcctcc atctcgcgca aggcatcggc gcagaattcc gccgccgaat
     3541 agctctctgt gggatcgcgg acatcgatca gccggtgcgg cgcgcgcgct tgttccgtgg
     3601 gcgtgggttt ggccgtgccg atatccatgc cgcgatacac ctgtgacgaa tccacgctga
     3661 tgatt
//
LOCUS       DBNGZE010000379         5449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_748203, whole genome shotgun sequence.
ACCESSION   DBNGZE010000379 DBNGZE010000000
VERSION     DBNGZE010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_748203"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC16FV_09665"
     CDS             <1..635
                     /locus_tag="AC16FV_09665"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR2850357.1"
                     /translation="APAPGTEMKGAAVTTPLADAAKTDAKNTATDSVSGTDTTSVKDA
                     PETASRETYIIQPGDVLTISVWREKDLQGEMAVRPDGGLNFPLVGEIVAAGKTIEQLK
                     TDIAAKLTKYVPDPVVTVTVKQAQGNKIYVVGKVNKPGDYFVNRNVDVMQALSMAGGP
                     NPYASVNKIKILRRVNGEQKTFLFKYSQVEKGNNLEQNIILQGGDIVVVP"
     gene            complement(890..2242)
                     /gene="prsR"
                     /locus_tag="AC16FV_09670"
     CDS             complement(890..2242)
                     /gene="prsR"
                     /locus_tag="AC16FV_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HIR2850358.1"
                     /translation="MQISNKLLVVEDDPGLQDQLRWCFDRYEVVVAGDRAAALEQLRK
                     HQPPVVTLDLGLPPDADGISEGLATLEEIVSQRPDTKVIVVTGQNDRENAVRAVGSGA
                     YDFYHKPIDAEILGMIVNRAYRLRELELENRVLMQQKSDHSVEGVVACSAEMVRVCRT
                     VEKVAPSNASILLLGDSGTGKELCARSLHDLSPRASGRFIAINCAAIPENLLESELFG
                     YEKGAFTGAVKQTPGKIEYASGGTLFLDEVGDLPMSLQAKLLRFLQERVVERVGGREE
                     IPVDVRVVCATHQNLPEKISSGTFREDLYYRISEIAVQIPPLRERGGDILLLARMYLE
                     HFNREQGRNLRGFSKEALTAMEAYSWPGNVRELKNRIKRAVIMAEDKQIAARDLELEE
                     VELDEKSLDLRHVREEAERQAILRALSHSAGKVSAAADMLGISRPTMYDMIRKLNIKV
                     "
     gene            complement(2252..4354)
                     /gene="prsK"
                     /locus_tag="AC16FV_09675"
     CDS             complement(2252..4354)
                     /gene="prsK"
                     /locus_tag="AC16FV_09675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HIR2850359.1"
                     /translation="MNVGVISYLTATGIFLVLVAVLLIGRTDGAVKRLLVVASAATAL
                     WAAGAAYQAANGTPLILPQLLELLRDFAWLTFLLHMLHAKTTEKGAMSTWLALVRHGL
                     YAVTVVTMLFALYHHFNVTGLTLLSGFDFQLAGHLTLAIAGLVLLEHILRNTPADLMR
                     AIKYLCFGIGGMFVYDFYLYSDALLFQRIDPALWEARGFINATVVPVIGIALARNPQW
                     SPGVLVSRRIVFHTSALLGSGIYLLAMGVGGYYVRDYGGTWGIVAQTIFFFGAGLLLL
                     ILLFSGPLRARLRVFINKHFFHYKYDYREEWLRFIRTLSSNETDIPLPERAIRAIAQM
                     IDSPGGVLWMRREDGHYEPVAKWNMGPPLSANEPAGSSLIRFLEIQKWVINLDEYRRD
                     PGLYQSLGDLVMPSWLRTLPNAWLIAPLILQEKLHGFVVLARSPAVRRHFNWEDCDLL
                     KTTGTQAASHLAQHAASQALAEARQFEAYNRLSTYVVHDLKNLISQLSLVVSNAARHR
                     NNPLFMENVISTVENSVSKMNRLLTRMREGVQADSRRMVNMAKLLEEAVHDARANNPV
                     PVLECAIGDLAVAADRDRLASVMGHVIRNAQDATSEDGRVTVRLQKVNGHAVVEVEDT
                     GCGMDEAFIRARLFRPFQTTKGDSGMGIGVYEAREFVRSLGGDIQVESKPGKGTIFRI
                     QLPHQPALEPVRYQQTQH"
     gene            complement(4837..>5449)
                     /locus_tag="AC16FV_09680"
     CDS             complement(4837..>5449)
                     /locus_tag="AC16FV_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850360.1"
                     /translation="QDAEKELRRAIEINPKWSAPYLSMAAIYTAQKNFPRAIAAIEEG
                     LQAIPDDPTLTLLLASTHEDNKAYDKAIDAYERVLKKSPDNTLAINNLASLLVEHRND
                     KKSLEQAKTLALKLEKSPQPVFRDTLGWVYYKTDEIDKAVPVLEAVVKEAPKIPIFNY
                     HLGMAYYKKGELPSAKTHLTKAVAVKGDYPGLEEARETLKKIP"
BASE COUNT         1202 a         1601 c         1463 g         1183 t
ORIGIN      
        1 cggcgcccgc gccgggtact gagatgaagg gtgctgccgt tacgacccca ttggcggatg
       61 ctgccaagac tgacgcgaaa aacaccgcca cggattctgt gtcgggcact gacacaacat
      121 ccgtaaaaga tgccccggaa accgcctctc gtgaaaccta catcatccag cccggcgacg
      181 tgctgaccat ttcagtatgg agagaaaaag acctgcaagg cgagatggcg gttcgccccg
      241 atggcgggct caatttccct cttgtcggtg aaatcgtcgc cgccggtaaa actattgagc
      301 agctgaaaac ggatatagcc gccaagctga ccaaatacgt tcccgatccg gtcgttaccg
      361 tcaccgtcaa gcaggctcag ggcaacaaaa tttacgtggt tggcaaggtc aacaaaccgg
      421 gtgactactt cgtcaatcgc aacgtcgatg tcatgcaggc gctcagcatg gcgggcgggc
      481 cgaatcctta cgcctccgtg aacaagatca agattctgcg tcgggttaac ggcgaacaga
      541 agacttttct gttcaagtac tcacaagtgg agaaaggcaa taatctggag caaaacatca
      601 ttctgcaagg cggcgatatc gtggtcgtgc cgtaagacgg acattcgctg atcggccggt
      661 tttgctgtcg ccctccatcc atctgcatgc atgcaagctc tggctgaaca tctcccggct
      721 gccaggtcga aaattcatac tgtgccggct cgaagtcagc cttgcccttt tgattgaggg
      781 gaagatggga aagagttgaa gacggggaag accactcggc cgaacactcc tttccggcct
      841 tctccgcccg cgtcagggag aaaggagctt acattcgccg gacttatttt tatactttaa
      901 tattcagctt ccggatcatg tcgtacatag tggggcggct aatgccgagc atgtcggccg
      961 cagcgctgac cttgccggcg gagtggctca gggcccgcag aatcgcctga cgttccgcct
     1021 cttcgcgtac gtgacgcaaa tccagactct tctcatccag ctccacctcc tcgagctcca
     1081 aatcccgggc cgcgatctgt ttgtcctccg ccatgatcac cgcgcgcttg atgcggtttt
     1141 tcagctcgcg cacgttaccc ggccagctgt aggcctccat ggcggtcagc gcctccttgc
     1201 tgaagccgcg caaatttcgt ccctgctccc ggttgaaatg ctcgagatac atgcgcgcca
     1261 gcagcagaat atcgccaccc ctctcccgca acggtggaat ctggacggct atctcgctga
     1321 tgcggtagta gagatcctcg cggaaagtcc ctgagctgat cttctccggg agattctgat
     1381 gggtagcgca gacaacgcgc acatcgacgg gaatctcctc gcgccccccg acccgctcta
     1441 ctacacgctc ctgcaggaag cgcaacagct tggcctgcag cgacatgggc agatcgccca
     1501 cctcatccag aaacagcgtt ccgccgctgg cgtattcgat cttgccgggt gtttgcttga
     1561 cggcgccggt gaaggcacct ttttcatagc cgaagagctc gctttccagc aggttttcgg
     1621 gaatggctgc gcaattaatg gcaatgaaac gaccgctggc ccgtgggctc aagtcatgca
     1681 gggaacgcgc gcagagttcc ttaccagtgc cgctgtcgcc cagaagcagg atcgaggcgt
     1741 tcgagggcgc gaccttctcc accgtgcgac ataccctgac catttccgcg ctgcaggcca
     1801 ccacgccttc cactgaatga tcgcttttct gctgcatgag tacgcgattt tccagctcca
     1861 gctcgcgcag ccggtaggcg cggttgacga tcatgccgag aatctccgcg tcaatcggct
     1921 tgtgatagaa gtcataggcg ccggaaccga cggcgcgcac ggcgttctcg cggtcgttct
     1981 ggccggtgac cacgatcacc ttggtgtcgg ggcgctgcga gacgatttct tccagggttg
     2041 cgaggccttc gctgatgcca tcggcgtcgg gaggcaggcc cagatccagt gtcacaacag
     2101 gcggctggtg cttgcgcagc tgctccaggg cggcggcacg gtcaccggcc acgacgacct
     2161 cgtaacggtc aaagcaccaa cgcaattgat cctgcaggcc ggggtcgtct tccaccacca
     2221 gcagcttgtt gcttatctgc ataatggccg gttaatgctg cgtctgttga tatcgcaccg
     2281 gttcaagagc aggttgatgc ggcaattgga tgcggaaaat cgtccccttg cccggtttgc
     2341 tttccacctg gatatctcct cccagagagc gcacgaactc gcgcgcttcg tatacgccga
     2401 tgcccatgcc ggagtctccc ttggtcgtct ggaacggacg gaacaggcgg gcgcggatga
     2461 aggcctcgtc catgccgcaa ccggtatcct ccacttccac caccgcatga ccattgacct
     2521 tttgcagccg gacagtgacc cgcccgtctt ccgatgttgc atcctgggca ttgcggatca
     2581 cgtgccccat caccgatgcc agccggtcgc ggtcggccgc gaccgccaag tcgccgatgg
     2641 cgcactccag aacaggtacc gggttattcg cccgtgcatc atgtacggcc tcttccagca
     2701 atttggccat attaaccatc cgccgggagt cggcttgcac gccttcacgc atgcgtgtca
     2761 ggaggcgatt catttttgaa accgaatttt ccactgtgct gatgacgttc tccatgaaca
     2821 gcggattgtt cctgtgccgg gcggcgttgg atacgaccag cgacaactgg gaaatcaggt
     2881 tcttgagatc gtgcacaaca taagtggaaa ggcggttata tgcctcaaac tgccgggcct
     2941 ccgccaaggc ttgggaagcg gcgtgctgcg ccagatggct ggccgcctgg gttccggtgg
     3001 ttttaaggag atcgcagtcc tcccagttga agtgacggcg caccgcggga gagcgcgcca
     3061 gcaccacaaa accgtgcagt ttctcctgca atataagcgg tgctatcaac caggcgtttg
     3121 gcaaggtccg cagccagctc ggcatcacca ggtcgcccag gctctgatac agacccggat
     3181 cacgccggta ttcatccaga ttgatgaccc atttctggat ttccaggaac cggataagcg
     3241 agctgcccgc cggctcgttg gcggacaatg gcggccccat gttccacttg gccacgggct
     3301 cgtaatggcc atcctcccga cgcatccata atactccgcc ggggctgtcg atcatctggg
     3361 caatcgcccg gatagcccgt tccggcaggg gaatgtccgt ttcgttcgac gacagggtgc
     3421 gaataaaccg cagccactcc tcgcggtagt cgtatttgta atggaaaaaa tgcttgttga
     3481 tgaagactct cagacgcgca cgtaacggtc ccgaaaacag caggatcaag agcaacaatc
     3541 ctgcgccgaa gaaaaaaatg gtctgtgcga caatgcccca ggtgccgcca tagtcgcgga
     3601 cgtaataacc gccgaccccc atggcaagca gataaatccc gctgcccaac agcgcggagg
     3661 tatgaaagac gattctccgg gataccagaa cgcccggcga ccattgcgga ttcctggcca
     3721 acgctatgcc gatgacaggc actaccgtgg cattgatgaa gccgcgcgct tcccatagtg
     3781 cgggatcgat acgctgaaac aacagggcat cggaatagag ataaaaatcg tacacgaaca
     3841 tgccgccaat gccgaaacac agatacttga tggcgcgcat caggtccgcc ggcgtattgc
     3901 gcaggatgtg ctccagcagc accaagcccg cgatggcaag tgtcaaatgg ccagccagtt
     3961 ggaaatcaaa tcccgacagg agggtcaatc cggtgacatt aaagtggtga taaagcgcaa
     4021 acaacatcgt taccactgtc actgcataaa ggccatgacg cacaagggca agccaggtgg
     4081 acatcgcgcc cttttccgtg gtcttggcat gcagcatgtg cagcaagaat gtcagccacg
     4141 caaaatcgcg caagagttcg agaagctggg ggagaatcag gggggttcca ttggcggcct
     4201 ggtaggcggc gccggcggcc cataacgcgg tcgccgcact ggccacgacc aacagacgtt
     4261 tgaccgcgcc atcggtgcgg ccgatcagca atactgctac aagcacgaga aaaatgcccg
     4321 ttgcggtcag gtagctaatc acaccgacat tcatgtttga tcagcaccaa cgcatacgac
     4381 aaccggaaac caatctctgc ataagtctat caatacctat acgacatttt tgtgtcagtc
     4441 ccaaccgaaa caaaaaagcc gccgtccgac ttttaaccgc tctcgccggt cagcttcatt
     4501 tcgggctgac aatccatgcg gaacaacgca atttattttt attccaggtc tcttgtacgc
     4561 ctttgcgctc gtaggggctt cctgccatcc ttcctcaaca caaccgggcc cgagcacacc
     4621 cgatgtcatt aacaccttaa aaacttctag tgaaccaata atatacctga aatcaggatt
     4681 cgataaagcg catttccagc cagaagtttc cttataattt tcagtctatt gagcgatttt
     4741 ttgaagaaac tggataagcc cgggtaaacg gctttttcca aacgccggta tatggggctg
     4801 tccaccatcg acagcccttt ttctgtctcg cccgcgctac gggattttct tgagcgtttc
     4861 ccgcgcctct tcgagccccg gataatcacc cttgaccgct accgccttgg tcaagtgggt
     4921 cttggcggat ggaagctcgc cttttttgta atacgccatg ccaagatgat aattgaaaat
     4981 gggaattttt ggtgcttcct tgacgacggc ttccagcacg ggaacagcct tgtcgatttc
     5041 atcagttttg taataaaccc aacccaatgt atcgcgaaaa accggttgcg gacttttctc
     5101 gagttttaga gccagcgttt tcgcctgttc aaggcttttt ttgtcgtttc tgtgttcgac
     5161 gagcaatgaa gcaagattgt tgatcgctag cgtgttatca ggacttttct tcaagacacg
     5221 ctcataggcg tcaatagcct tgtcgtatgc tttattatct tcgtgcgtac tcgcgagcaa
     5281 tagagttaac gtaggatcat ccgggatggc ctgcaaaccc tcttcaatgg ctgcgatcgc
     5341 acgtggaaag ttcttttgcg ccgtatatat cgccgccatg ctgaggtatg gcgcgctcca
     5401 tttcgggttt atctcaatcg cacgacgaag ttccttctct gcatcctga
//
LOCUS       DBNGZE010000380         6732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_192257, whole genome shotgun sequence.
ACCESSION   DBNGZE010000380 DBNGZE010000000
VERSION     DBNGZE010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_192257"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC16FV_09685"
     CDS             complement(<1..394)
                     /locus_tag="AC16FV_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR2850361.1"
                     /translation="MSQDFNDPAIKDPRPSSPVVPVKLGLDDSFQFRCRKGIACFNKC
                     CQNINIMLTPYDILRLKNRLGLTSAEFLEKHTTTFEMDAHGMPGIRMKTKPGGAECQF
                     LTPEGCGVYEDRPAACRYYALGATTMRAT"
     gene            complement(509..2431)
                     /gene="aprA"
                     /locus_tag="AC16FV_09690"
     CDS             complement(509..2431)
                     /gene="aprA"
                     /locus_tag="AC16FV_09690"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HIR2850362.1"
                     /translation="MAEGTFGNPQVVEEEVDILIIGGGMAACGAAYEIGQWTEAAKAK
                     GHNIKVKLVDKAALDRSGAVAQGLSAINTYCGENDPADYVRYVRNDLMGITREDLVYD
                     VGRHVDDSVHHFEEWGLPVWKQPGDEGKKLTEGGKPVRSGKWQIMINGESYKWIVAEA
                     AKKALGVENIQERIFIVRLVTDKNDPTRVAGAAGFSVRENKLFIYKFKACLLVCGGAV
                     NVFRPRSVGEGTGRAWYPVWNAGSTYAMAAECGAELTMMENRFVPARFKDGYGPVGAW
                     FLLFKAKVMNGAGEDYNALRGDVIKDEKRFGKYGQGVPGTCLRNHVMLQEMRDGRGPI
                     MMDTPTALANLAKNMTPKEVQHLLAEAWEDFLDMTIGQCGIWAGENIEPDKKPSEIMP
                     TEPYLLGSHAGCAGIWVSGPTDVGAPKEWSWGYRSMTTVKGLFTAGDGVGASGHKFSS
                     GSHAEGRIAAKGMVKFVMDNKDWKPEMSRTTQELVDEIYRPVKTFLEHKDYTTAIDIN
                     PHYITPKMLQFRLQKIMDEYCAGVSTYYQTNAKMLEICEAKLEMLKEDSLKMRAKDLH
                     ELLRAWENYHRILAAEAHMKHIQFRQETRYPGYYYRADYNFVDDQNWKVFVNSKYDRN
                     THKWEMKKVPYVQLVK"
     gene            complement(2510..2974)
                     /gene="aprB"
                     /locus_tag="AC16FV_09695"
     CDS             complement(2510..2974)
                     /gene="aprB"
                     /locus_tag="AC16FV_09695"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HIR2850363.1"
                     /translation="MPTFVRTDKCDGCKGQDKTACMYICPHDLMKLDKDGSETGHAMK
                     AWNQEPEQCWECYSCVKICPQQAIECRHYADVVPLGGSVQPLRGTDSIMWTIKFRNGN
                     IKRFKFPIRTTAEGSIKPYDGKPKADVSKIDEIDFFTANSIHKTDRSQLVSG"
     gene            complement(3047..4258)
                     /gene="sat"
                     /locus_tag="AC16FV_09700"
     CDS             complement(3047..4258)
                     /gene="sat"
                     /locus_tag="AC16FV_09700"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR2850364.1"
                     /translation="MSKLVNPHGGGELKPLLLKDAALIAEKKRAESLPKVRITSRETG
                     DVIMMGIGGFTPLAGFMTKADWQGVCDGMKMANGLFWPIPITLSTDKATADGIQTGTD
                     VALVDSESGEILATMKVTEKYSIDKAHECMMVYKTTDLEHPGVKMVMEQGDVNLAGPI
                     KVLSTGTFKQEYGDQFLTPAETREMFKKNGWSTVAAFQTRNPMHRSHEYLAKVAIETC
                     DGVLIHSLLGKLKPGDIPADVRSKAIGTLIDKYFVKNTVIQAGYPLDMRYAGPREALL
                     HALFRQNYGCSHLIVGRDHAGVGSYYGPFDAHHIFDEIPKDALETKALKIDWTFWCYK
                     CGGMASARTCPHDEKDRLLLSGTKLRKMLSEGGDVPSEFSRPEVLEVLRAYYGSLTEK
                     VEVKLSGHSAR"
     gene            4588..4824
                     /locus_tag="AC16FV_09705"
     CDS             4588..4824
                     /locus_tag="AC16FV_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR2850365.1"
                     /translation="MSITVRFFASIRERLGKEQDIIESKGITCAADVWNRAAKIPLPA
                     NTLVAVNMEYARPDQPVRDGDEVAFFPPVTGGMQ"
     gene            4821..5258
                     /locus_tag="AC16FV_09710"
     CDS             4821..5258
                     /locus_tag="AC16FV_09710"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR2850366.1"
                     /translation="MKILLLEKAFDPWQEIARHQSEQPALAGKFGATTVFVGTLRDFN
                     QGSDVQAMTLEHYAPMTRKHLERVAGEALARWPIEDVLVIHRYGELRPNDPIVCVAVW
                     AAHRDAAFDACRYLIDELKTRAPFWKQEQTPQGRRWVAPEEME"
     gene            complement(5271..5933)
                     /locus_tag="AC16FV_09715"
     CDS             complement(5271..5933)
                     /locus_tag="AC16FV_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850367.1"
                     /translation="MLPRKLACLLILGLFLPVTGALAEKDTTIKKCKDATGRWHYGDT
                     AANECAKSKITVMSDEGTTKKVIAAPPTEQELKDREARRESIEAEQARVEEQKRKDAL
                     LLSTYGVEDDIIYIRDRKIAQIEASVKASEETLKPLRAALTRLEAQATDESKDAKAAE
                     ATAKNIELTRNQIARHEGAVAVKRQEQEALRKQYAEELERYRELKKKAPAAVSSSAAA
                     KK"
     gene            6313..>6732
                     /locus_tag="AC16FV_09720"
     CDS             6313..>6732
                     /locus_tag="AC16FV_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2850368.1"
                     /translation="MNLTEQLILLLVSLVANVFSAFAGGGAGLLQFPALIFLGLPFGV
                     ALATHKVASVALGVGATLRHLHEKSLEWKFSLFILAAGLPGVIVGAVFILDVPDDLAR
                     LALGVLTIGLGRYSFVNKQLGRTPAPLPRTRPGYALGG"
BASE COUNT         1141 a         1984 c         2112 g         1495 t
ORIGIN      
        1 cggtggcgcg catggtggtg gcgccgaggg cgtagtagcg gcacgcggcc ggccggtctt
       61 cgtacacgcc gcagccttcg ggcgtgagga actggcattc ggcgccgccg ggcttggtct
      121 tcatgcggat gccgggcatg ccgtgcgcgt ccatctcgaa cgtggtcgtg tgtttttcca
      181 ggaactccgc cgaggtcagc ccaaggcggt ttttaagccg caggatgtcg tagggcgtga
      241 gcatgatgtt gatgttctgg cagcacttgt tgaaacaggc gatgcctttg cggcagcgga
      301 actggaagct gtcgtccagg ccgagcttca ccggcacgac cgggctcgac gggcgcgggt
      361 ccttgatggc ggggtcgttg aaatcctgac tcatggcatc acctcgaata cggccggcgg
      421 gaatacagcc ggcattttaa ctcaattcac taaagacaaa ggggtccggg tttccccgga
      481 ccccttcatt tactgactta ccgactgatt acttgaccag ctgcacgtag gggactttct
      541 tcatctccca cttgtgcgtg ttgcggtcgt acttcgagtt cacgaagacc ttccagttct
      601 ggtcgtccac gaagttgtaa tccgcgcggt agtagtagcc cggataacgc gtctcctgac
      661 ggaactggat gtgcttcatg tgcgcctcgg cggcgaggat gcggtggtag ttctcccacg
      721 cgcgcaggag ttcatgcagg tccttggcgc gcatcttgag agagtcttcc ttcagcatct
      781 cgagcttggc ttcgcagatc tcgagcatct tggcgttggt ctggtagtag gtcgacacac
      841 ccgcgcagta ctcgtccatg atcttctgca gacggaactg cagcatcttg ggcgtgatgt
      901 agtgcgggtt gatgtcgatc gccgtggtgt agtccttgtg ttccaggaag gtcttcaccg
      961 gacggtagat ctcgtccacc agttcctgcg tcgtgcgcga catctcgggc ttccagtcct
     1021 tgttgtccat cacgaacttg accatgccct tggcggcgat gcggccttcg gcatgcgaac
     1081 cggaggagaa cttgtgaccg gaggcgccca cgccgtcacc ggcggtgaac aggcccttga
     1141 cggtcgtcat ggagcgatag ccccagctcc attccttcgg tgcgccgacg tcggtcgggc
     1201 cggagaccca gataccggcg cagccggcgt ggctgcccag caggtacggc tcggtcggca
     1261 tgatttcgga gggcttcttg tccggctcga tgttctcgcc ggcccagatg ccgcactggc
     1321 cgatggtcat gtcgaggaag tcttcccagg cttccgccag caggtgctgc acttccttcg
     1381 gggtcatgtt cttcgcgagg ttcgcgagcg cggtcggggt gtccatcatg atcgggccac
     1441 ggccatcgcg catttcctgc agcatgacgt ggttacgcag acaggtgccc ggcacgcctt
     1501 ggccgtactt gccgaagcgc ttctcgtcct tgatgacgtc gccacgcagg gcgttgtaat
     1561 cctcgcccgc gccgttcatc accttggcct tgaacagcag gaaccatgcg cccaccggac
     1621 cgtagccgtc cttaaagcgg gcgggcacga agcggttttc catcatggtc agttcggcgc
     1681 cgcactcggc cgccatcgcg taggtcgaac cggcgttcca caccggatac cacgcacggc
     1741 ccgtgccttc accgacggag cggggacgga acacgttcac cgcgccgccg cacaccagca
     1801 ggcaggcctt gaacttgtag atgaagagtt tgttctcgcg caccgagaaa ccggcggcgc
     1861 cggccacgcg ggtcgggtcg ttcttgtcgg tgaccagacg cacgatgaag atgcgctcct
     1921 ggatgttttc cacgcccagg gccttcttcg cggcttcagc gacgatccac ttgtaggatt
     1981 cgccgttgat catgatctgc cacttgccgg aacgtaccgg cttgccgcct tcggtgagtt
     2041 tcttgccttc gtcaccgggc tgcttccaca ccggcagacc ccactcctcg aagtgatgca
     2101 ccgagtcgtc cacgtgacgg ccgacgtcgt acaccaggtc ttcgcgggtg atgcccatca
     2161 ggtcgttacg cacgtagcgc acatagtcgg ccggatcgtt ctcgccgcaa taggtgttga
     2221 tggcggacag accctgcgcc acggcgccgg agcggtcgag cgcggccttg tccacgagct
     2281 tcaccttgat gttgtggccc ttggccttgg cggcctcggt ccactgtccg atttcataag
     2341 cggcgccgca agcggccatg ccgccgccga tgatgaggat gtcgacttct tcctcaacga
     2401 cctgcggatt tccaaatgta ccttctgcca tgatattcac cttcaattta cggcgatcgt
     2461 cccggggcac tcgtcccctc gcggccgatg cgccggttgg ttgttgaaat taaccgctga
     2521 ccagctggct gcgatccgtc ttgtggatgc tgttggccgt gaagaaatcg atctcgtcga
     2581 tcttgctgac gtcggccttg ggcttgccgt catacggctt gatcgagcct tccgcggtgg
     2641 tgcggatcgg gaacttgaag cgcttgatat tgccgttacg gaacttgatg gtccacatga
     2701 tcgaatcggt gccacgcagc ggctgcaccg agccgccgag gggaacgacg tcggcgtaat
     2761 gacggcactc gatcgcctgt tgcgggcaga tcttcacgca ggaatagcat tcccagcact
     2821 gctccggttc ctggttccac gccttcatcg catggccggt ttcggaaccg tccttgtcga
     2881 gcttcatcag gtcgtggggg cagatgtaca tgcaagcggt cttgtcctga cccttgcagc
     2941 catcgcattt gtcggtacgt acgaatgttg gcatggtgtc actccatcta tctaaggttg
     3001 caattaaaaa cggttaaaac ggttaacgaa ctcttgcttc tgaaatctat cgggccgaat
     3061 ggccggaaag cttgacctcg accttctcgg tcaggctgcc gtagtaggcg cgcagcactt
     3121 cgagcacctc ggggcgcgag aactcagagg gcacgtcgcc gccctcggac agcatcttgc
     3181 gcagcttggt gccggacagc agcagacggt ccttctcgtc atgcgggcag gtgcgggccg
     3241 aagccatgcc gccgcacttg tagcaccaga aggtccagtc gatcttgagt gccttggttt
     3301 cgagcgcgtc tttcgggatt tcgtcgaaga tgtgatgtgc gtcgaacggg ccgtagtagc
     3361 tgccgacgcc ggcgtggtca cgcccgacga taaggtgcga gcagccgtag ttctggcgga
     3421 atagcgcgtg cagcagcgcc tcgcgcggac cggcgtagcg catgtccagc gggtaaccgg
     3481 cctggatcac ggtgttcttg acgaagtatt tgtcgatgag cgttccgatg gccttggagc
     3541 gcacgtcggc cgggatgtcg ccgggcttga gcttgccgag cagggagtgg atcagcacgc
     3601 cgtcgcaggt ttcgatcgcg accttggcaa ggtattcgtg ggagcggtgc atcgggttgc
     3661 gggtctggaa cgccgcgacg gtggaccagc cgttcttctt gaacatctcg cgggtttcgg
     3721 ccggggtcag gaactggtcg ccgtattcct gcttgaacgt gccggtggag agcaccttga
     3781 tcgggccggc gaggttcacg tcgccctgtt ccatcaccat tttgacgccg ggatgttcca
     3841 gatcggtcgt cttgtacacc atcatgcact catgcgcctt gtcgatggag tacttttcgg
     3901 tgaccttcat cgtggccagg atctcgccgg actcgctgtc cacgagcgcc acgtcggtgc
     3961 cggtctggat gccgtcggcg gtggccttgt cggtggagag ggtgatcggg atcggccaga
     4021 acaggccgtt agccatcttc atgccgtcgc acacgccttg ccagtcggcc ttggtcatga
     4081 acccggcgag cggagtgaag ccgccgatgc ccatcatgat gacatcgccg gtctcgcgtg
     4141 atgtgatgcg gaccttgggc agggattccg cgcgtttctt ttcggcgatg agtgccgcgt
     4201 ctttcagaag caacggtttc agttcgccgc cgccatgtgg attgaccagt ttagacataa
     4261 atgtcgccct actttgttcg tttggacata attaggaatc ctcttcccct tatgtcggat
     4321 ttgattgaaa tttatgatgt cccgacgtct gagccgcgga actctatagt gttgaagtcc
     4381 gcaaacctaa ttagaattac tgaggccgca tataagtttc acttatttgc ggtgggtgat
     4441 accttgatcc ctggaaacga ggactgcttt gctgtggatc ggagcgccag tgtcggcgaa
     4501 cccacagcgg gccctattta gggaggccgg ctattctaac gcagtttgcc ttgccgtcca
     4561 ccctgtcgag catgaggtaa ccaaatcatg agtattacgg tcaggttctt tgccagtatt
     4621 cgcgaaaggc tggggaaaga acaagacatt atcgaaagca agggtattac ctgcgctgcg
     4681 gatgtgtgga accgcgcggc gaaaatcccc ctgccagcca acacactggt ggcggtcaac
     4741 atggagtacg cgcggccgga tcaaccggta cgcgatggcg atgaggtggc gttctttccg
     4801 cccgtgaccg gaggcatgca gtgaaaattc tgctgttgga aaaggctttc gatccctggc
     4861 aggaaatagc gcgccatcag tccgaacagc ctgccttggc cgggaaattc ggcgcgacga
     4921 cggtattcgt cggcacgttg cgggatttca atcagggcag tgatgtgcag gccatgacgc
     4981 tcgaacatta cgcgcccatg acccgcaagc atctggagcg cgtggccggg gaggcgctgg
     5041 cgcgctggcc gatcgaggac gtgctggtga tacatcgtta cggcgagtta cgcccgaacg
     5101 atccgatcgt gtgcgtggcg gtatgggcgg cgcaccgcga cgctgccttc gacgcctgcc
     5161 gttaccttat tgatgaattg aaaacacgcg cgccgttctg gaaacaggaa cagacgccac
     5221 aggggcggcg ctgggtggcg ccggaagaaa tggagtaatg cgcgttgtaa ttatttttta
     5281 gccgcagcgg aggaggaaac cgcggcgggt gctttcttct tcagttcacg atagcgctcg
     5341 agctcctcgg cgtattgttt gcgcagcgct tcctgctcct gacgcttgac cgcgaccgcg
     5401 ccttcatgcc gcgcaatctg gttgcgggtc agttcgatgt ttttcgccgt ggcctcggcc
     5461 gccttggcat ccttgctttc atccgtggcc tgcgcttcca ggcgcgtgag cgcggcacgc
     5521 aacggtttca gtgtttcttc gctcgccttc acggaggctt cgatctgcgc aatcttgcgg
     5581 tcgcggatat aaatgatgtc gtcctccacg ccataggtgg acagcagcaa cgcatccttc
     5641 cgcttctgtt cctcgacgcg ggcctgttcc gcctcgatgc tttcccgtct cgcctcacga
     5701 tctttcaatt cctgctccgt cggcggcgcg gcgatcactt tcttggtcgt gccttcgtcg
     5761 ctcatcaccg tgatcttgga tttggcgcat tcattggcgg cggtgtcgcc ataatgccag
     5821 cggccggtgg catccttgca cttcttgatg gtcgtgtcct tttcggccag ggcgccggtg
     5881 acaggcagga acagacccag gatcaacagg caggcaagtt ttctcggcag catgaaatag
     5941 cgactcctta atatcactct ttcaagatag gccccgcttt gcggatgtgt caaccgcggc
     6001 cgccgtccgg caggcggccg ccgagaagtg gtacatgggt gagccgcgag gcggcgaacg
     6061 ggcgcacagg gaagtgcgcc ccggactttt tcgcgaagta gggtgagcgg cgaacttagg
     6121 agccgcgaac gcccgaccac ggacggtcgg gccggacttc gagcgaagca cggaaggcga
     6181 agcgagaagg cgcagcggaa aagtaccgtt tacggagcta aggatgcaag gacgaccggg
     6241 tccgggtggc gtggcatctg tgccataatc cgcggccatg actcccggga aactccgtcg
     6301 ggcggcgttc cggtgaacct gaccgagcag ctgatcctgc tgctggtgtc gctggtcgcg
     6361 aacgtgtttt ccgcgttcgc cggcggcggc gccgggttgc tgcaatttcc ggcgctgatt
     6421 tttctcggcc tcccctttgg cgtggcgctc gcgactcaca aggtcgccag cgtggcgctc
     6481 ggcgtgggag cgacgctgcg gcacctgcac gaaaaatcgc tcgagtggaa attctcgctg
     6541 ttcattctcg ccgccggtct gcccggtgtg attgtcggcg ccgttttcat tctcgatgtg
     6601 ccggacgatc tcgcgcgcct cgcgctcggc gtgctcacga tcggcctcgg gcgttattcg
     6661 ttcgtcaaca agcagctcgg gcggacgcct gcgcccctcc ctcgcacgcg gcctggctat
     6721 gccctcggcg gg
//
LOCUS       DBNGZE010000381         5553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1651135, whole genome shotgun sequence.
ACCESSION   DBNGZE010000381 DBNGZE010000000
VERSION     DBNGZE010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1651135"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="AC16FV_09725"
     CDS             complement(<1..721)
                     /locus_tag="AC16FV_09725"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR2850369.1"
                     /translation="MKKTMWLVVLLVLGGIAAGFYYWQMGQEEPLPAPPPVTAPPAPP
                     SPPEPQIRHPIKEAAPSEKPLPTLETSDDAMVEALTGFFGQKPVQEFFDVKEVVRRFV
                     VTVDNLPRQKVPLRYRLFKPVAGQFLATGQDEDYLTNPDNHRRYKSYVLLADAVDAKK
                     LVAVYTRFYPLFQQEYMNLGYPKGYFNDRLVEAIDDLLAAPEIPGRIKLARPKVMYLF
                     ADPALEELSAGQKIMIRMGVEN"
     gene            complement(822..1274)
                     /locus_tag="AC16FV_09730"
     CDS             complement(822..1274)
                     /locus_tag="AC16FV_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018876950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR2850370.1"
                     /translation="MSFPVQAADVLNTKIMTLDLARDIANHTLKACRKNGYNTAVVVV
                     DRSGDAQVVLRDVYVSRHAVEIAERKASAVVMSGVSTQDFRTNRADIRTELNEVRGIL
                     LLRGGLPIRSGGSLIGAVGVSGAPGGEKDEACAAAGIKAVQERLDFVE"
     gene            complement(1459..1896)
                     /locus_tag="AC16FV_09735"
     CDS             complement(1459..1896)
                     /locus_tag="AC16FV_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850371.1"
                     /translation="MSSAQERARELAREMKKAIMEAKTAEARAKRLGDEVLLALAEAR
                     KEIQAASEIVEYPVGRYECKRCGQSSIFSQTYRELPPCDNCGSTEYLGAEPTITKITP
                     PPPKKYQAGMYECTGCRTRIVLPEDMDELPPCDICGGHKLKTV"
     gene            2128..2358
                     /locus_tag="AC16FV_09740"
     CDS             2128..2358
                     /locus_tag="AC16FV_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850372.1"
                     /translation="MRKEQATDAGFPGIAFATLPKTGETVAIRYGERLFYRVDSAKTA
                     DELNAIYGVTARQAQEVLASALAGWKAPPANS"
     gene            complement(2417..3217)
                     /locus_tag="AC16FV_09745"
     CDS             complement(2417..3217)
                     /locus_tag="AC16FV_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850373.1"
                     /translation="MKNISQDLLRRLPGLAQDWHAGEFDAAVFAAQYFLYWQMASHGA
                     QFASRKNRNDPRPMAPEWLAMMETAKGESLRNHMLDWLEHYQFRGVISNVPAALVQWL
                     RGAWSLILREDVPTPLEVLRMQARGRRAVTILTEYPRLCEPVLHKPDAFAFFVHDLGH
                     AYKFFHSPALCAGQCGFFAALEDAYDRDVFASYLDNPEFVARFHYLMSDMNTHPEHGR
                     QYLRAILVECHLRREQKAPSEPLSSMAERTIEEVMRAMGGSASLAACA"
     gene            complement(3231..4253)
                     /locus_tag="AC16FV_09750"
     CDS             complement(3231..4253)
                     /locus_tag="AC16FV_09750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR2850374.1"
                     /translation="MNIDELKSVARSIVAKQKGVLAADESSPTIKKRFDSIKVESTEE
                     NRRRYREILFTTDGIERYIGGVILFDETLRQSTHDGTPFAKLLSSRGIVPGIKVDKGA
                     KALALCPGDKVAEGLDGLRERLAEYKQLGARFAKWRAVIEINERDVPSAFGIRANAHG
                     LARYAALCQEIDLVPIVEPEVLMDGAHDIERCEVVTSRVLEAVFAELDAHHVVFDGML
                     LKPNMVIPGMKCPNQASVQQVAEATLHCLRRYVPAAVPGIVFLSGGQSAEDATDHLNA
                     MNAMGPQPWQVSFSYGRALQAPVLAAWKGQESNVVAAQKALLKRCQLNGLARDGKYSR
                     AMEQAA"
     gene            complement(4425..4643)
                     /locus_tag="AC16FV_09755"
     CDS             complement(4425..4643)
                     /locus_tag="AC16FV_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850375.1"
                     /translation="MKKLLTSLMALAFIAGLGFSASSMAMKHMAEATAKACKDKKPGD
                     MVKVDGKDVKCPEPKKEEAKEEKKEMKK"
     gene            complement(4830..>5553)
                     /locus_tag="AC16FV_09760"
     CDS             complement(4830..>5553)
                     /locus_tag="AC16FV_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR2850376.1"
                     /translation="LPAALRANAPVHIHLTGGTDVRAAPPLDYLRFVLLPLLTQMGAA
                     VQLTLQRRGYYPRGGGVVELMVQPRALKGLNLETAGAVKEIRGIAHVSNLPAHIVTRM
                     QQTAVRLLADIAHVDIEQQTLGKDQAIGQGGAIVLWARMAHTLLGASEVAQRGVPAER
                     IAGTAAQALREDIVAGATLDLHAADQILIYCTLAQGPSTFLARLFTPHAQTTLWLLQQ
                     FLPLRASTTPAGAATRVDMEPG"
BASE COUNT         1078 a         1720 c         1596 g         1159 t
ORIGIN      
        1 cattctcgac acccatgcgg atcatgatct tctgccccgc cgacagttct tcaagggcgg
       61 gatccgcgaa caggtacatg accttgggtc tagccagctt tatccgtccc gggatctccg
      121 gcgcggccaa caggtcgtca atggcctcca ccaggcggtc gttgaagtaa cccttgggat
      181 atcccaggtt catgtattcc tgctggaaca gcggatagaa gcgcgtatac accgcgacca
      241 gttttttggc gtccacggcg tcggccaaca acacatagga cttgtagcgc cggtgattgt
      301 ccggattggt gagatagtct tcgtcctggc cggtggcgag aaattgcccc gccaccggtt
      361 tgaacagccg gtaacgcagc ggcaccttct gacgcggcag attgtccacc gttaccacga
      421 agcgccgcac cacctccttt acatcgaaga attcctggac gggcttttga ccgaagaaac
      481 ccgtgagcgc ctcgaccatg gcgtcatcgc tggtttccag cgtcggcaga ggcttttccg
      541 agggcgccgc ttccttgatt ggatggcgta tctgcggctc gggcggtgac ggcggtgcag
      601 gcggcgccgt taccgggggc ggtgcgggca gcggttcttc ctggcccatt tgccaatagt
      661 aaaaaccggc ggcgataccg ccgagcacaa gcaagaccac cagccacatc gtttttttca
      721 tgacagatgc cccgctacct gaaagagtca atcaaactct caagcctcat cttacgacgc
      781 ggccaacgtg aacccaagct tggactcgat cggtcgccat cctattccac gaaatcgagc
      841 cgctcctgca cggccttgat gccggccgcg gcgcaggcct cgtccttctc gccgcccgga
      901 gcgccgctga cgcccaccgc gccgatcaaa gaaccgccgg agcggatcgg cagtccgccc
      961 cgcagtagca ggatgccgcg tacctcgttc agctcggtgc ggatatcggc gcgattggta
     1021 cggaagtcct gcgtgctgac gccggacatg accactgcgc tcgccttgcg ctcggcgatt
     1081 tcaacggcat ggcgcgagac gtaaacgtcg cgcagcacca cctgcgcatc gcccgaacgg
     1141 tcgaccacca ccactgccgt gttgtaaccg ttcttgcgac aggccttcag cgtatgattg
     1201 gcgatatcac gcgccagatc gagcgtcatg atcttggtat tcagtacatc cgccgcttga
     1261 accgggaaag acatgatggc gcccagcagt aacggtacaa atcgttttgt ttccatggca
     1321 atttctccga ttgggaaagg cgcggtgtgt atgcaccgcg ctgtcggaat cagacaacgc
     1381 gacaaatcat attattcatc catgatttcg ggaagttttt cggctttcac gaggcggtat
     1441 atcgcttgat gatgcggctt acaccgtctt gagtttgtga ccgccacaga tgtcgcacgg
     1501 tggcaactcg tccatatcct ccggcaggac gatgcgcgtg cggcagccag tacattcata
     1561 catgccagct tgatattttt tgggtggcgg cggcgtgatt ttcgtgatcg tcggttcggc
     1621 gccgagatat tcagtgctgc cgcagttgtc gcagggtggc agctcccggt aggtttgcga
     1681 gaagatcgag ctttgcccgc agcgcttgca ttcatagcgg ccgaccggat attcgacaat
     1741 ctcgctggct gcctgtattt ccttcctggc ctcggccagg gccagcagca cctcgtcccc
     1801 caggcgcttg gcgcgggcct cggcggtctt ggcctccatg atggcctttt tcatttcgcg
     1861 ggccagttct ctggcccgtt cttgcgccga tgacatgttg tctcccctgc cggctctgaa
     1921 tcaggtttgt ttcaggtttg cttgtcgccg ttcgtcaacg gcatccgacg tgttatcggc
     1981 tgcccataaa caagcggcgc attgtcatga cactataaca gacgtagtgc agactgcatg
     2041 gtttctggcc acggacgcgt tacggcgtca gggatgaaac ccatgatctc atgatgagct
     2101 catgtcacac gcaacaggag gacgaccatg cgtaaagaac aggccaccga tgccggtttt
     2161 cccggaatag cgtttgccac cctgccgaaa accggcgaga ccgtcgcgat tcgttacggc
     2221 gagcgtctct tctaccgggt cgactcggcc aagaccgcgg acgaactgaa cgcgatatac
     2281 ggcgtcaccg cgcgtcaggc acaagaagtt ttggcgagtg cgttggccgg ctggaaagcg
     2341 ccgccggcga attcgtagga ccgcgacgcg cggccattct agtcaacccg ccctgcttca
     2401 aggaattatc cgaccgtcac gcgcaggccg ccagggatgc gctgccgccc atggcgcgca
     2461 tcacctcttc aatcgttcgt tccgccattg aagacagcgg ctccgaaggc gccttttgct
     2521 cgcggcgcag atggcattcc accagaatcg cgcgcagata ttgccggccg tgttccgggt
     2581 gcgtgttcat gtcgctcatc aggtaatgaa accgggcgac gaattcggga ttgtccagat
     2641 acgacgcaaa cacatcgcga tcataagcat cttcgagtgc ggcaaaaaat ccacattgac
     2701 cggcacacaa cgccggtgaa tggaaaaact tgtaggcatg tcccagatcg tgcacgaaaa
     2761 aggcaaaggc atcgggcttg tgcagcaccg gctcacacaa ccgcggatac tctgtgagta
     2821 ttgtcaccgc gcgcctgccg cgcgcctgca ttcgtaacac ctccagcggt gttggaacgt
     2881 cctcgcgcag gatcaaggac cacgcaccac gcagccattg aaccaaagcc gccggcacat
     2941 tgctgatcac gccgcgaaac tggtaatgct ccagccaatc gagcatgtga ttgcgcaaac
     3001 tctccccttt cgccgtttcc atcatggcca gccattccgg tgccatgggg cgcggatcgt
     3061 tcctgttttt gcgcgaggcg aattgtgccc cgtgtgatgc catttgccag taaagaaaat
     3121 actgagccgc aaataccgct gcatcgaact ctccggcatg ccagtcttgc gccaggccgg
     3181 gaagccgccg caacaaatct tgcgaaatat ttttcatgct ctccggtggc ctatgccgcc
     3241 tgttccatgg cgcgggaata ttttccgtcg cgcgccagcc cgttcagctg acaacgtttc
     3301 agcagcgcct tctgcgcggc gacgacgttg ctttcctgcc ccttccacgc cgccagcacc
     3361 ggcgcttgca gcgcccgtcc gtaggaaaaa ctgacctgcc acggttgtgg gcccatcgcg
     3421 ttcatggcgt tcagatgatc ggtggcgtcc tccgcgctct gtccgccgga caggaacacg
     3481 atcccgggga ccgccgccgg cacgtaacga cgcaggcagt gcagcgtggc ctcggccacc
     3541 tgctgcacgc tggcctgatt cggacatttc atgccgggaa tcaccatgtt cggtttgagc
     3601 agcatgccat cgaacaccac atggtgggca tccagttcgg cgaataccgc ctccagcacc
     3661 cgcgatgtca ctacttcaca acgctcgata tcgtgggcgc cgtccatgag cacttccggt
     3721 tccacgatcg gcaccaggtc aatctcctgg cacagcgcgg cgtaacgcgc gagtccgtgg
     3781 gcattggcgc ggatgccgaa cgccgacggg acatcgcgtt cattgatctc gatcaccgcg
     3841 cgccacttgg cgaatcgggc gcccagttgt ttgtactcgg cgagccgctc gcgcaggcca
     3901 tccagaccct cggcgacctt gtcgcccggg cacagtgcca gcgccttggc gcccttgtcc
     3961 accttgatgc cgggcacgat gccgcggctc gatagcagct tggcgaaagg tgtaccgtcg
     4021 tgcgtgctct ggcgcagagt ttcatcaaaa agaatgacgc cgccgatata acgttcgata
     4081 ccgtcggtgg taaaaaggat ttcgcggtaa cgccgccggt tttcctcggt ggattcgacc
     4141 ttgatcgaat cgaaccgctt tttgatcgtg ggactgcttt cgtccgcagc cagcacaccc
     4201 ttctgtttcg cgacgatgga tcttgccact gacttgagtt cgtcgatgtt catggctgct
     4261 accctccggc gatggcgctc ttcagcgagg atgaaatgaa tatcgcggat aaaatcctaa
     4321 cataacgcca atcagccgac tttgtcattc gcgccgggga gcgggaacaa aaacgcctcc
     4381 gattggaggc gttttaggaa gcagaatgaa gtaacgacta ttgcttattt cttcatctcc
     4441 ttcttctctt ccttggcttc ttccttcttc ggctccgggc acttcacgtc cttgccatcg
     4501 accttcacca tgtcgcccgg cttcttgtcc ttgcaggcct tggcggtggc ttcggccatg
     4561 tgcttcatgg ccatgctgga ggcgctgaaa cccaggccgg cgatgaaggc aagggccata
     4621 agactcgtca gtaatttctt catgcgtagt gctcctaagg aaatgaatag ttgaacctgg
     4681 aatatggcaa ggaggtaaaa accggcttcc ctgcacgata aaactgtaac gttcaggcga
     4741 cggggcgtca acgcattggt ataaagccga agccccaggc cataaacagc ctcaggccgg
     4801 gcgttttgcg gcctgcggtg acaggcaagt tagccaggct ccatgtccac gcgcgtggcg
     4861 gcgccggccg gcgtggtact cgcgcgcaat ggcaaaaact gctgtaacag ccaaagcgtg
     4921 gtctgtgcat ggggcgtgaa caggcgcgcc agaaaagtgg acgggccctg cgccagcgtg
     4981 caataaatca ggatctgatc ggcggcatgg agatcgagtg tcgccccggc gacgatgtct
     5041 tcgcgcagcg cctgtgcggc ggtaccggct atgcgctccg cgggcacgcc gcgctgcgcc
     5101 acttcggagg cgccgagcag cgtgtgtgcc atacgtgccc acagcacgat ggcgccaccc
     5161 tgccctatcg cctgatcctt gcccagtgtt tgttgttcga tgtcaacatg ggcgatatcg
     5221 gcgagcagcc gcacggctgt ctgctgcatg cgcgttacga tatgtgccgg taaattggag
     5281 acatgagcga taccgcggat ttcttttacc gctcccgccg tttcgagatt cagtcccttg
     5341 agggcgcgcg gctgcaccat gagctccacc accccgccgc cgcgcggata gtagccgcgc
     5401 cgctgaagcg tcagctgcac cgccgcgccc atctgtgtca gcagcggcag cagcacaaac
     5461 cgcagataat ccagcggcgg cgcggcccgc acatcagttc caccggtgag atgaatgtgc
     5521 accggtgcgt tggcacggag cgccgcaggc agc
//
LOCUS       DBNGZE010000382         5335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1234185, whole genome shotgun sequence.
ACCESSION   DBNGZE010000382 DBNGZE010000000
VERSION     DBNGZE010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1234185"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2401)
                     /gene="fdnG"
                     /locus_tag="AC16FV_09765"
     CDS             complement(<1..2401)
                     /gene="fdnG"
                     /locus_tag="AC16FV_09765"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1811..1813),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HIR2850377.1"
                     /translation="MVSISRRQFLKLSAAGLSASSLAILGFSPNQVLAEARQFKLART
                     TETRNTCPYCSVSCGVLIYSLGDKAKNSPAEIIHIEGDPDHPVNRGTLCPKGAGLLDF
                     VHSPNRLKYPEYRAPGSDKFVRISWDDAFDKIARLMKSDRDKNFIAKNADGATVNRWL
                     STGFLAASASSNETGFLTHKVVRSLGMLAFDNQARVUHGPTVASLAPTFGRGAMTNHW
                     VDIKNANLILIMGGNAAEAHPCGFKWVTEAKAHNKAKLIVVDPRFTRSAAVADYYAPV
                     RAGADIAFLAGVINYLLTNDKIQHEYVKAYTDVSFIVRADYSFEDGLFSGYNAAKRSY
                     DKSTWMYELGADGYVKTDPTLANPRCVFNLMKQHFARYTPEVVSNITGTPKEQFLKIC
                     EMIAETSAPNRTLTVMYALGWTQHSVGSQMIRTAAMVQLLLGNIGMSGGGMNALRGHS
                     NIQGLTDLGLLSNLLPGYMTLPGEKETDYKAYIEKRTFKPLRPGQLSYWQNYPKFHVS
                     LMKAWYGDAATKDNNWAFDYLPKLDKVHDILQVFEDMYQGKMNGYFCQGFNPLASFPC
                     KAKIDGSLSKLKYLVTIDPLATETAVFWQNHGEYNNVDSSKIQTEVFRLPSTCFAEED
                     GSLVNSGRWLQWHWKAAEPPGEAKSDAEIMAGIFLKLRELYRKEGGAFPDPILNLTWG
                     YKMPYAPSPEEIAKEFSGKALADIPDPKEKGKVLRKSGEQLDGFAQLQDDGTTACGCW
                     IFAGAWSEKGNLMARRDNSDPSGLGNTLNWAFAWPANRRVLYNRASCDPSGKPWDPKR
                     KL"
     gene            complement(2661..3737)
                     /gene="mtnA"
                     /locus_tag="AC16FV_09770"
     CDS             complement(2661..3737)
                     /gene="mtnA"
                     /locus_tag="AC16FV_09770"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR2850378.1"
                     /translation="MSTPASQTKGIPYDKIRAVEWENGRLKLIDQRILPGKFEHLYFD
                     HLPDAARAITDMVVRGAPAIGVTAAYAVVLAARDRYKENPNGWQKAIQADLNHLAEAR
                     PTAVNLRWAVEHMRGLINAGIPGNPVLRLLSEAQRIHAEDIAANRRMGELGAALIEKG
                     SGVLTHCNTGALATGGYGTALGVVRAGYAAGKIKNVYADETRPWLQGARLTAWELVQE
                     GIPATLIADSAATYLMKQGKVAWVIVGADRIAANGDTANKIGTYMTAVAARHHGVKFM
                     VVAPTSTVDMNTRDGAAIPIETRAEAEVLNFGGTRTAPEGIQAWNPAFDITPAELIDA
                     IVTEKGVVANPTRKKMTAMMASPG"
     gene            4105..4734
                     /locus_tag="AC16FV_09775"
     CDS             4105..4734
                     /locus_tag="AC16FV_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR2850379.1"
                     /translation="MQPLLLSGLIAGGYLIASPQSFAALPSTGVGYVHDSSGIVVRSS
                     NGNCVRTSSWSPETATAQCDPELVQRAAAPASSEPAASAPAEIAAIESRHEPFSLGEK
                     AQFDFDQAVLKAEDKQRLDAAITQLKTLPEDATIRITGYTDNIGSEEYNLGLSMRRAE
                     AAQEYLVNKGIDRKRIVIAGMGASNPVASNATAEGRAMNRRVEVVAESK"
     gene            complement(4994..>5335)
                     /locus_tag="AC16FV_09780"
     CDS             complement(4994..>5335)
                     /locus_tag="AC16FV_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850380.1"
                     /translation="PNSTNPDLPYKWTKENQRVATKHGPQLSHLKNILSHPQVRLIVD
                     PDKTGVEEADPYVLALAAYLQEKGYEITVITQETKDRPDKLSMGTACGLLRLPRLPLA
                     AYLVQQKIWPP"
BASE COUNT         1058 a         1571 c         1557 g         1149 t
ORIGIN      
        1 tgagcttgcg cttcggatcc cacggcttgc cggagggatc gcaggaggcg cggttgtaga
       61 gaacgcgccg gttggccggc caggcaaacg cccagttgag cgtgttgccg agcccgctcg
      121 gatcggagtt gtcgcgccgc gccatgaggt tgcccttctc cgaccacgcc ccggcgaaga
      181 tccagcagcc gcaggcggtg gtgccgtcgt cctggagctg agcgaagcca tccagttgct
      241 cgccgctctt ccgcagcacc ttgcctttct cctttggatc ggggatgtca gccagcgcct
      301 tgccgctgaa ctccttcgcg atctcctcgg gcgacggggc atacggcatc ttgtagcccc
      361 aggtgagatt cagaatcgga tccgggaatg caccgccttc cttcctgtag agttcacgca
      421 gtttcaggaa gataccggcc atgatctcgg catcggactt ggcctcgccc ggcggctcgg
      481 cggccttcca gtgccactgc agccagcggc cggaattcac cagcgaaccg tcttcctcgg
      541 cgaagcaggt ggacggcaga cggaacacct cggtctggat cttggaggaa tccacgttgt
      601 tgtactcgcc gtggttctgc caaaacaccg cggtctcggt cgcgagcgga tcgatcgtca
      661 ccagatattt gagcttggag agcgaaccat ctatcttggc cttgcagggg aacgaagcga
      721 gcggattgaa tccctggcag aagtagccat tcatcttccc ctgatacatg tcctcgaaca
      781 cttgcaggat gtcgtgcacc ttgtcgagct tcggcagata atcgaacgcc cagttattgt
      841 ccttggtcgc ggcgtcgcca taccacgcct tcatcaggct cacgtggaac ttgggatagt
      901 tctgccagta gctgagctgc ccgggacgca agggcttgaa cgtgcgcttc tcgatgtagg
      961 ccttgtagtc ggtttccttc tcgcccggca gcgtcatgta accgggcagc agattggaca
     1021 gcaggccgag gtcggtaagg ccctggatgt tggagtggcc gcgcagcgca ttcatgccgc
     1081 cacccgacat gccgatgtta ccgagcagca gctgcaccat ggccgcggtg cggatcatct
     1141 gcgatcccac ggagtgctgc gtccagccga gcgcgtacat gacggtcaag gtacggttcg
     1201 gcgccgaggt ctcggcgatc atctcgcata tcttgaggaa ctgctccttg ggcgtgccgg
     1261 tgatgttgct cacgacctcg ggcgtgtagc gcgcgaagtg ctgcttcatg agattgaaca
     1321 cgcagcgcgg gttagcgagc gtcgggtcgg tcttgacata gccgtccgcg cccagttcgt
     1381 acatccaggt ggacttgtcg taactgcgtt tggccgcgtt gtaaccggag aacaggccgt
     1441 cctcgaacga gtagtcggcg cgcacgatga aactcacgtc ggtgtacgcc ttgacgtact
     1501 cgtgctggat cttgtcgttc gtaagcaggt agttgatgac gccggccaga aacgcgatgt
     1561 cggctccggc gcgcaccggg gcgtaatagt cggccaccgc cgccgagcgc gtgaaacgcg
     1621 gatcgacgac gatcagcttg gccttgttgt gcgccttggc ttcggtcacc cacttgaaac
     1681 cgcacgggtg ggcttcggcg gcgttgccgc ccatgatcag gatcaggttg gcattcttga
     1741 tgtcaaccca gtgattggtc atcgcaccgc ggccgaatgt cggggcaaga cttgccaccg
     1801 tggggccgtg tcagacgcgt gcctggttgt cgaatgccag catcccgagg ctgcggacga
     1861 ccttgtgcgt gaggaaaccg gtctcgttgc tcgaggccga agccgcaagg aagcccgtac
     1921 tgagccatcg gttgaccgtc gcgccgtccg cgttcttcgc gatgaaattc ttgtcgcgat
     1981 cgctcttcat gagacgcgcg atcttgtcga aagcgtcatc ccaggagata cgcacgaact
     2041 tgtccgagcc aggtgcgcgg tattccggat atttgagccg gttggggctg tgcacgaaat
     2101 cgagcaggcc cgcgcccttg ggacagagcg tgccgcgatt cacgggatga tccggatcgc
     2161 cctcgatgtg aatgatctcg gccggagagt tcttggcctt gtctccgagg ctatatataa
     2221 ggacgccgca actcacggaa caataagggc aggtgttgcg agtctccgtg gtgcgcgcca
     2281 gcttgaattg gcgcgcctcg gcgagtactt ggttgggcga gaatcccaga atcgcgaggc
     2341 tcgatgcgct gagtccggcg gcactgagct tgaggaactg ccgtctggat atagatacca
     2401 tggcgtgtct cctaatcctt cggtttttta cgtcctcccg tttttttatg acaccatttt
     2461 ccagctgaaa taccctatac ccgcggtcaa caaaaagcaa cgaacgcccg cctcctgacg
     2521 ctggggtttt caggtttgaa agagatgaaa atttcacgtg atgaaaaatg cacggggctg
     2581 gaatcccgga ccgtaccgcc aaacaaccat atttaattat ttaatgatat tgctcagcag
     2641 gtcataccgg gatgacaaac ttaaccaggg ctggccatca ttgccgtcat ctttttgcgg
     2701 gtgggatttg cgaccacgcc tttttcggtc acgatggcgt caatcagctc cgccggggtg
     2761 atatcgaagg ccggattcca ggcctgtatg ccctccggcg ccgtgcgcgt gccgccgaag
     2821 ttgagcacct cggcctcggc gcgggtctcg atagggatcg ccgcaccgtc gcgggtattc
     2881 atgtccacgg tgctggtggg cgccaccacc atgaatttca cgccgtggtg gcgcgccgcc
     2941 accgccgtca tgtaggtgcc aatcttgttg gcggtgtcgc cgttggcggc gatgcggtcc
     3001 gcccccacga tcacccacgc caccttgccc tgcttcatca aataagtcgc ggcgctgtcg
     3061 gcgatcaggg tcgccgggat cccttcctgc accagctccc acgccgtcag gcgcgcgccc
     3121 tggagccatg ggcgggtctc gtcggcataa acatttttaa tcttcccggc ggcataaccg
     3181 gcgcgcacca cccccagcgc cgtgccgtaa cctcccgtag ccagcgcccc ggtgttgcag
     3241 tgcgtcagaa cgccactgcc cttttcgatc agcgccgcgc ccagctcgcc catgcgccgg
     3301 ttggcggcga tatcctcggc gtggatgcgc tgcgcttccg aaagcagccg caacaccgga
     3361 ttgcccggaa tgccggcatt gatgaggccg cgcatatgct ccaccgccca acgcagattc
     3421 accgccgtcg ggcgtgcttc ggcaaggtga ttcaaatccg cctgaatcgc tttctgccag
     3481 ccattggggt tttccttgta tctgtcccgc gccgccagca cgacggcata agccgccgtc
     3541 acgccgattg ccggcgcccc gcgcaccacc atgtcggtga tcgcccgggc agcatccggg
     3601 agatggtcga aataaagatg ttcgaatttg ccggggagga tgcgctggtc gatgagtttc
     3661 aaccgaccgt tttcccattc gacagcgcgg attttgtcgt agggaattcc tttagtttgt
     3721 gaggcgggag tgctcatggg aagtgagtat accgtatcgt cattcccgcg ggagcggggt
     3781 gaggcggagt atttgtgaat acagttaacg tgtattcacg ccgccgaagc cgctcctgcg
     3841 catccctgcg cccgcggcat tcgcacatcc ttgtgcagca cgggtgcaac gaattctgtt
     3901 gcaccctggg cagcaacagg aaagaacgaa tcgagcccga tttctaaaac accactgaaa
     3961 gcagagaaat gcaacttcga gcgaaataat tgtcataaaa cataccgcag catgacgacc
     4021 tgccccgcac aggctgtgca tttgaaagaa gcagcggggt tttattcatc gcaaatatag
     4081 gagaaaccaa acatggatgg aaaaatgcaa cctctgttat tgtccggact gatagccggc
     4141 ggttacctga tcgcatctcc gcaaagtttt gccgccctgc cgtcgacagg cgttggctac
     4201 gttcatgaca gctccggaat cgtcgtgcgc agcagcaatg gcaattgcgt gcgcacatcc
     4261 agctggagtc cggaaacggc cacggcccaa tgcgaccccg agctggtaca acgcgccgcc
     4321 gcaccggcct ccagtgaacc cgcggcgtca gcaccagccg aaatcgccgc catcgaatcc
     4381 cggcatgaac ccttcagcct cggcgagaaa gcccagttcg atttcgatca ggcggtgctg
     4441 aaagcggaag acaagcaacg gctcgacgca gcgattaccc aactgaaaac tcttcccgaa
     4501 gacgcgacca tccggatcac gggctacacc gataacatcg gcagcgagga atacaacctg
     4561 ggcttgtcga tgcgacgcgc cgaagccgcg caggagtatc tggtcaataa aggcatcgat
     4621 cgcaagcgca tcgtgatcgc gggcatgggc gcctcgaatc ccgtggcgag caacgccacc
     4681 gccgaaggcc gggccatgaa tcgccgggtc gaggttgtgg ccgaatcgaa gtaagcagta
     4741 aggcgcgatc agcaggtatc ggtagttcgc gctgagcaat cgtggggtgg tgcatcaccc
     4801 cacgatatct tttgctgtct cttgtggttg tcattcccgc agaggctccc tttgccgtca
     4861 ttcccgcgga agcgggaatc caggtcttgc ataaacaaaa gccgcctttg gcggcttttg
     4921 acttttaaac cgtggtctgt acctatttgt gcaacccctt taattaaacc tggttccttt
     4981 aattatttat taactaaggg ggccatattt tttgttgcac taaataagca gctagaggca
     5041 aacgtggcaa acgcaacaag ccacatgctg tgcccataga aagtttgtct ggacgatcct
     5101 tcgtttcctg ggtgattacc gtaatctcgt agcctttctc ctgcaaataa gccgctaacg
     5161 ccaaaacata tgggtcagct tcttcaacac ctgtcttatc tggatcaaca atcaatctga
     5221 cctgcggatg actcaatata tttttcagat ggctaagttg tgggccatgt tttgtagcca
     5281 ccctctgatt ttcttttgtc cacttataag gtaaatctgg attcgtagaa tttgg
//
LOCUS       DBNGZE010000383         4079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_818437, whole genome shotgun sequence.
ACCESSION   DBNGZE010000383 DBNGZE010000000
VERSION     DBNGZE010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_818437"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="AC16FV_09785"
     CDS             <1..177
                     /locus_tag="AC16FV_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HIR2850381.1"
                     /translation="GLSDLTAGVRLRYEIRREFAPYIGIERSGKFGGTADFIRAESKP
                     AKETRVVAGLRFWF"
     gene            347..775
                     /locus_tag="AC16FV_09790"
     CDS             347..775
                     /locus_tag="AC16FV_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2850382.1"
                     /translation="MDHGKMQMDDQGDMAAMHGHWMAPEEAAKRPNPVKANSASRARG
                     KKLFAANCAGCHGRAARGDGPAGAALNPKPADLTVMAGQHPDGDFAWKIAEGRGAMPA
                     WKHALKDEQIWDLVNYIQGLAKLKKIHPSVDGHQHNHQHY"
     gene            907..1362
                     /locus_tag="AC16FV_09795"
     CDS             907..1362
                     /locus_tag="AC16FV_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2850383.1"
                     /translation="MKRIVTVVVAALLVGACGKEKPTGKDAASALQGDRPITTESLFR
                     GVRLFHEHCAQCHGPEAQGHPDWRNPKVVAAPPLNGAGNVWKRRKQDMIAIIKNGVKH
                     NGDPVMPAWQGRLTDREIEDIITWYQALWPPEVYERWRKANVPESVPSG"
     gene            1661..2077
                     /locus_tag="AC16FV_09800"
     CDS             1661..2077
                     /locus_tag="AC16FV_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850384.1"
                     /translation="MTGTQFKKWFTVRHRQAIAMAVAGWMSLSLVLAFWPCCQAMAQD
                     QPPITHVEAGDHDHGNMPQGTDDPCRTWLDTADAALNTAPDVLLSNPELKVGHAVYAV
                     AHDFPAAVASTPGRHLYHPSPPDPLPLYLRVQHLLI"
     gene            2241..2762
                     /locus_tag="AC16FV_09805"
     CDS             2241..2762
                     /locus_tag="AC16FV_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850385.1"
                     /translation="MTTVSKLSKQSSVTPTWTAWKTFASLIWRWAILALGLHLLWEIG
                     QLPFYTVWEEANAWRIALYVTHCVLGDVTIATLTYLAVALAWRQVDWPRQRPWVGGTM
                     LVALGFGYTVFSEWYNVYRVGAWAYAESMPRILGIGLTPLLQWLVVPVAMLMLVRHAR
                     IRDGVSGPGRMNR"
     gene            2809..2940
                     /locus_tag="AC16FV_09810"
     CDS             2809..2940
                     /locus_tag="AC16FV_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850386.1"
                     /translation="MYHDGYMSWGMGFSWMGILFWIVLILAVAALAKYLFGKPGGRD"
     gene            3031..3423
                     /locus_tag="AC16FV_09815"
     CDS             3031..3423
                     /locus_tag="AC16FV_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR2850387.1"
                     /translation="MYGFNVQLKDSFDNSVKRVTEALKTEGFGVLTDIDVQATMKAKL
                     GIDGKPYRILGACNPPLAHRAITADPDIGLLLPCNVVVREESDRNITVGFMDPIAVLK
                     LTDNPAITELANEVRGKLDRVCNRLVAG"
     gene            3502..3984
                     /locus_tag="AC16FV_09820"
     CDS             3502..3984
                     /locus_tag="AC16FV_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850388.1"
                     /translation="MKNLITVITVAAALLAASAAPAVEEHHPDQKPAAAPTTQAPPKD
                     PEQIAKQLKENADKLRAQADKIAKTKDPAERQKLVQEHMQTLRAGMGMASGMMGGMGA
                     GPGGGMGMGMMGGGMMDCPMMGQMMGGGMMGEQMMQRMQQMEKRMDMMQMMLEQMRKP
                     "
BASE COUNT          888 a         1070 c         1297 g          824 t
ORIGIN      
        1 ggcttgtccg acctgacggc cggcgtgcga ttacgttacg aaatccggcg tgaattcgct
       61 ccttacatcg gcatcgagcg gagcggcaaa ttcggcggca ccgcggattt tatccgcgcg
      121 gagagtaagc cagcgaagga aacacgggtg gtggcgggcc tgcgcttctg gttttgaagc
      181 gtggcatata accatttttt catttcaagg aacaatgtaa atgatacgag tgaatgcaaa
      241 tttttttcgc caagcgatgt tgctatggcc actgattgtt ttgccatcga ttgcgctcgc
      301 agatgccgaa catgacgcaa agaaagtgcc ggttacgatg gaaggcatgg atcacggtaa
      361 gatgcaaatg gatgaccagg gcgatatggc tgccatgcac ggtcactgga tggcaccgga
      421 ggaggcggcc aagcggccca atccggtgaa agccaatagc gcctcgcggg cgcgcggcaa
      481 gaaactgttc gcggcgaact gcgccggttg tcatggccgc gcggcacgcg gcgacgggcc
      541 tgcgggcgcg gcgctcaatc ctaaaccggc cgatttgacg gtcatggccg ggcagcatcc
      601 ggatggggat ttcgcctgga agatcgccga gggtcgtggc gccatgccag cctggaagca
      661 tgcgctcaag gacgagcaga tctgggattt ggtgaactac atacaaggcc tcgccaagct
      721 gaagaaaatt catccgagcg tggatggcca tcagcacaat catcaacatt attgagcagt
      781 tgattggtga gcattgacgg ttcggtcgcc cggtcggttc gtcccgaggc aggaggtgat
      841 tttctttgtg gcggacaaca attattatta acgacaatgg ccgacaggta ttgcggttcc
      901 aatcacatga aacgaatcgt gacggtggtg gtagccgcat tgctagttgg cgcttgcggc
      961 aaggaaaagc cgaccggaaa ggatgccgcc tccgctcttc agggcgatcg gcctattacc
     1021 accgagagcc tgttccgggg ggtcaggctg tttcacgagc actgcgccca gtgccacgga
     1081 ccggaggccc agggccatcc cgattggcgg aatccgaaag tagttgccgc gccgccactc
     1141 aacggggcgg gtaacgtatg gaaacgccgc aaacaggata tgattgcgat cattaaaaac
     1201 ggcgtcaaac acaacggcga tcccgtcatg ccggcgtggc agggccggct gaccgaccgg
     1261 gaaatcgagg acatcattac ctggtatcag gcgctgtggc cgccggaagt gtacgagcgc
     1321 tggcgcaagg ccaatgtccc ggaatccgta ccgagtggat gaaagctgta gtccggttta
     1381 acacacataa aggtaaaaac gatgcaaatc gccatctgga tcatcatcat tttggtgatc
     1441 gccgcgctta tcaaatacct gctgaggggc gacgggaaaa aataggggag gcgtttcacc
     1501 gcggaagtgg tccggtcttt cctgatattt tttgcgatat caatgcgata tcaaatttga
     1561 taaatatcaa aaggaccgca tccggatatc gctaatattt ggcacacgaa atgtgaactt
     1621 ttgtcttctg gtggagtctc cttgaaaggg atgaaacaga atgacgggaa cccagttcaa
     1681 aaagtggttt acggttcgac accgccaggc gattgcgatg gcggtggccg gctggatgtc
     1741 cctgtcgctg gtgctggcct tctggccctg ttgtcaggcc atggcgcagg atcagcctcc
     1801 catcacccac gttgaggccg gagatcacga tcacggtaac atgccgcaag gtacggatga
     1861 tccatgccgt acctggctcg ataccgcgga tgccgcgctc aatactgccc ctgatgtcct
     1921 gctttcgaat cccgaactga aagtcgggca tgcggtatat gccgttgcgc atgatttccc
     1981 ggcagcggtc gcctccacgc ccggccggca tttgtatcat ccctcgccac ccgatcctct
     2041 accgctttat ctgcgtgttc aacatctgct gatctgagtt ctttcccgat tgcggcgagg
     2101 gcaagccgcg cccgggggag atgaggattt cggcagttcc ccgtttcctt ccgaagttgc
     2161 tttccccacg cctcggtccg tgtgtgacgt tgtattcgcg gtagttatgc atagttcacg
     2221 gcagagagag gaaaaatttc atgacgactg tcagtaagct atcgaaacaa agcagtgtca
     2281 caccgacatg gacggcatgg aaaacattcg catccttgat ctggcgatgg gcgattctgg
     2341 cgctggggct ccatcttttg tgggagatcg ggcaattgcc gttctacacg gtatgggaag
     2401 aagccaacgc atggcgtatc gcgctttatg tgacgcattg tgtgctgggc gatgtgacga
     2461 tagccacgct gacttatctg gccgtggcgc tggcttggcg gcaagtggat tggccgcggc
     2521 aacgaccttg ggtgggtgga acaatgctcg tcgcgcttgg gtttggctat acggtgttca
     2581 gcgagtggta caacgtttac cgcgtcgggg cctgggcata tgccgagtcc atgccgcgta
     2641 ttctgggaat cgggctcacg ccgctgttgc aatggctggt tgtgcccgtc gcgatgctca
     2701 tgctggtacg gcacgccagg attcgagacg gggtgagcgg tcccggcagg atgaatcgct
     2761 gacacgggca tgtgaaacat gagcaccaat gagtacagga ggtgacgtat gtatcacgac
     2821 ggctatatga gctggggcat gggcttcagc tggatgggca tcttgttctg gatcgtgctg
     2881 atcctcgcgg ttgccgcgct cgccaagtac ctgttcggca aacctggcgg acgcgattga
     2941 tcgttcggcg tttgcccgac gctgtgaggt tgccggcggg gagcatcgga cggtgattgt
     3001 gaaataactt tcaacgcaaa ggaggtacct gtgtacggtt tcaatgtgca actgaaggac
     3061 agcttcgaca actcggtgaa acgggtgacc gaggctctca agacggaggg gtttggcgtg
     3121 ctgaccgata tcgatgtgca ggccacgatg aaggccaagc tcgggatcga cgggaaaccc
     3181 tatcgcattc tcggcgcgtg caatccgccg ctggcgcatc gggcgatcac ggccgacccg
     3241 gatattggcc tgctgttgcc atgtaacgtc gtggtacggg aagagtctga tcggaacatc
     3301 accgtgggtt tcatggaccc gatcgcggtc ctgaagctga ccgacaatcc ggcgatcacc
     3361 gaactggcga acgaagtacg cggcaagctc gatcgcgtgt gtaaccggct ggtcgcaggc
     3421 tgatactgcg ccggcccgga tgcggggcaa gacatgcagg ttccgcacaa accctttcaa
     3481 caacgcttca aggagtcaac catgaaaaat ctcattacag tcattaccgt tgccgcggcg
     3541 ctgctggccg catccgccgc gccggccgtc gaggagcatc atccggacca gaaaccagcg
     3601 gccgcgccga cgacccaggc accgccgaag gatccggagc agatcgcgaa acagctgaaa
     3661 gagaatgcgg acaagctgcg cgcgcaggcg gacaagatcg ccaagaccaa ggacccggcc
     3721 gagcgtcaga agctcgtgca ggagcatatg cagacgctgc gcgccggcat gggcatggcg
     3781 tcgggcatga tgggcggcat gggcgccggc cccgggggcg gcatgggtat gggcatgatg
     3841 ggaggcggaa tgatggactg tccgatgatg ggccagatga tgggcggggg catgatgggc
     3901 gagcagatga tgcagcgcat gcagcagatg gagaaacgca tggacatgat gcagatgatg
     3961 ctcgaacaga tgcgcaagcc ctaggccggg cggccggccg acaacctgaa aaggaggctc
     4021 tatggaaaaa ctcaatccct gggttatggg cgcggcgctt gccatcacct tcgtcgcgg
//
LOCUS       DBNGZE010000384         2533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1721081, whole genome shotgun sequence.
ACCESSION   DBNGZE010000384 DBNGZE010000000
VERSION     DBNGZE010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1721081"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="AC16FV_09825"
     CDS             <1..1036
                     /locus_tag="AC16FV_09825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR2850389.1"
                     /translation="LGSGYVNPHALICARLVSRDPKYTLDQSLLTHRLNIALSLRERL
                     LDAPYYRHAFGDSDGLPGLVVDRYGDVAVVQITTAGMERLKSEIVAALQKVIRPAAIL
                     FRNDTSTRELEGLTSYVETALGEIPETIGLEENGVKFAVPLLTGQKTGWFYDQRLNRA
                     RFRHYIKGQRVLDVFSYLGAWGVQAAVAGAGSVLCVDSSPKAPEAIRHNADLNGVTDK
                     VTVEREDAFDILRRLRAARERFDVVVIDPPAFIKRKKDVKEGTQAYQRLNQMAMQVLS
                     KDGILVSCSCSYHLHRDDLRDILLKSSRHIDRFLEIIEEGHQGPDHPMHPAIPETGYL
                     KAFFARLLPS"
     gene            1280..1723
                     /locus_tag="AC16FV_09830"
     CDS             1280..1723
                     /locus_tag="AC16FV_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2850390.1"
                     /translation="MYKRILVAVDGSKTAQLALDEAIKLAKQWQAQLRIVHAVDMVSL
                     GWPEVGYDAAEVYESFRKAGRKVIEKAEESCRAAHVTAETKLAELDKLALHIADAIVA
                     EADAWPADLIVIGSHGRRGVSRMLLGSVAEGVARIASKPVLLIRG"
     gene            2022..>2533
                     /locus_tag="AC16FV_09835"
     CDS             2022..>2533
                     /locus_tag="AC16FV_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR2850391.1"
                     /translation="MKIHHLTPDEALASLRSRAEGLSAAEAARRLAEFGANRIERVRT
                     EHAGRRLLHGFTHFLAVILWLAAALAFFAEYREPGQGMSLLGVAILGVILINGIFSFW
                     QEHRAEQALAALQRLLPHQVKVTRENALLTLPAAELVPGDILWLEAGDDVPADCRVLE
                     AFGARVDNAT"
BASE COUNT          472 a          823 c          755 g          483 t
ORIGIN      
        1 gctcggttcg ggctatgtga atccgcacgc gctcatctgc gcgcggctcg tttcgcgcga
       61 tccgaaatac acgctcgacc agtcgttgct cacgcaccgg ctcaatatcg ccctgtcgct
      121 gcgggaaaga ttgttagacg ctccttatta ccggcacgcc ttcggcgaca gcgacggcct
      181 tccgggactg gtggtggacc gctatggcga cgtcgccgtg gtccagatca ccaccgccgg
      241 gatggaacgg ctgaaaagcg aaatcgtggc cgcgctgcaa aaagtcatcc gccccgccgc
      301 gatcctgttt cgcaacgaca cttccacccg tgaattggag ggactgacaa gctacgtcga
      361 gaccgccttg ggcgagatac cggaaaccat cgggctcgag gaaaacggcg tgaaattcgc
      421 cgtgccactg ctcaccggtc agaaaaccgg ctggttctac gaccagcgcc tgaaccgtgc
      481 gcgcttccga cactatataa aaggtcaacg cgtattggat gtgttcagct atctcggcgc
      541 ctggggcgtg caggctgcgg tcgctggtgc cggttcggtg ctgtgcgtgg acagctcccc
      601 aaaagcaccg gaggccatac gccacaacgc cgatctgaac ggtgtaacag acaaagtcac
      661 cgtcgaacgc gaggatgcct tcgatatact gcgccgcttg cgcgccgcgc gtgagcgctt
      721 cgatgtcgtg gtgatcgatc cacccgcgtt catcaagcgc aagaaagacg tcaaggaagg
      781 aacccaggcc tatcagcggc tgaaccagat ggcgatgcag gtgttgtcga aggacggcat
      841 cctggtgtcg tgttcctgct cctatcacct gcaccgcgat gatctgcgcg acatcctgct
      901 caagagcagc cggcacatcg atcgctttct ggaaatcatc gaggaaggac accaggggcc
      961 ggaccatccc atgcatccgg cgattccaga gaccggctat ctcaaggctt tcttcgcgcg
     1021 cctgctgccg agctgaaccg tcgctgaatc ttgtatgcca cgcggtttgc gtccctcggc
     1081 tccgtaatcg gtacatccat gtaccactcc tggcgtcctt cggcaccgca accggcacat
     1141 tgcatccttg gctccgtaat cggtgcatcc atgcaccact tctcctgtgc cgcggtttga
     1201 catgcgtcaa agtgagggac cgcgaattgc ttgaccatgg taatcgatcg aaacagattt
     1261 caaaccggag cgaaacacca tgtacaaacg tatccttgtc gcggtggacg gcagcaagac
     1321 agcccaacta gccctggacg aggccatcaa gctcgcaaag caatggcagg cgcaactgcg
     1381 catcgtgcac gccgtggata tggtttccct cggttggcct gaagtcggat acgatgccgc
     1441 ggaggtatac gagagtttcc gcaaggccgg tcgcaaagtt atcgagaaag cggaagagtc
     1501 gtgtcgtgca gcgcacgtca cggcggaaac aaaactggcg gagctcgata aacttgctct
     1561 ccatatcgcc gatgccatcg tcgccgaggc cgacgcttgg cccgccgatc tgatcgtgat
     1621 cggcagccac gggcgacgcg gcgtgagccg gatgctgctc gggagcgtcg cggagggcgt
     1681 ggcgcgcatc gcctccaaac cggtgctgct gatccgcggc tgaagggcat tcttcgcgcg
     1741 cccgctaccg ggctgaaccg ctacaccaat ttgcatccct cggcacctca atcagtactt
     1801 ttccgctgcg ctgtcctgct tcgcggaaat gcccggggcg caccttcgtc gctacgcgtc
     1861 ccgcttcgct cgaaggtcca ggcccggcca tccctggccg gaccgctcgc ggctttcgcc
     1921 gctcgcccct gtgcgcccgt tcgccgcttc gcggctcacc cctgtgccga ggtttgctgt
     1981 gtatcaaatc tcccggcgcg gaaacggcat agaatcgcgc cgtgaagata catcatctga
     2041 cgccggacga ggcgctcgcg agtcttcgca gtcgcgcgga gggattgtcc gccgccgaag
     2101 ccgcgcggcg gctcgcggaa tttggcgcta accgcatcga acgcgtgcgt acggagcacg
     2161 ccgggcggcg cctgctgcac ggtttcaccc atttcctcgc ggtcatcctc tggctcgccg
     2221 ccgcgctcgc cttcttcgcc gaataccggg aacccggtca gggcatgagc ctgctcgggg
     2281 tggcgatcct cggcgtaatc ctgatcaacg ggattttttc cttctggcag gagcaccgcg
     2341 cggagcaggc gcttgctgcg ctgcaacggc tactgccgca ccaggtcaaa gttacgcgcg
     2401 agaatgcgct gctcacgctt ccggcggccg agctcgttcc cggcgacatt ctctggctcg
     2461 aagctggcga cgacgttccc gccgactgcc gcgtgctcga agccttcggc gcacgtgtcg
     2521 acaacgccac cat
//
LOCUS       DBNGZE010000385         2373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_748863, whole genome shotgun sequence.
ACCESSION   DBNGZE010000385 DBNGZE010000000
VERSION     DBNGZE010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_748863"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="AC16FV_09840"
     CDS             <1..362
                     /locus_tag="AC16FV_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endoribonuclease YicC domain-containing protein"
                     /protein_id="HIR2850392.1"
                     /translation="KAKELYPEAARLFRERLEARLKEIRQQLDPARLEQEIVLFAQRA
                     DVAEEIDRLTAHFAEIRRVFTQPGPVGRRLDFLMQELNREANTLSSKSTDTRLTNVAV
                     ELKVLIEQMREQVQNIE"
     gene            473..1081
                     /gene="gmk"
                     /locus_tag="AC16FV_09845"
     CDS             473..1081
                     /gene="gmk"
                     /locus_tag="AC16FV_09845"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR2850393.1"
                     /translation="MNNKGKLFIFSAASGTGKTSLAKALVESMPDVAFSVSHTTRAPR
                     PGEQHGVHYYFVTPQQFDEMVAADRFLEHAKVFGNFYGTSRAATENLLWQGKNIIFDI
                     DWQGARAIKEKMPEAVSVFILPPSRAALEERLTGRGPDASEVIARRMREAVSEMSHYK
                     EFDHLVVNDDFNAALADLKAIIRGEGSPRPVRVDMEALLAEG"
     gene            1246..1551
                     /gene="rpoZ"
                     /locus_tag="AC16FV_09850"
     CDS             1246..1551
                     /gene="rpoZ"
                     /locus_tag="AC16FV_09850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR2850394.1"
                     /translation="MARITVEDCLDNVDNRFQLVLVATRRARQLIAGADTFVPRENDK
                     PTVIALREIADGYVTNAILDEEAPPAGLEADENTADQPQAGGLDASPAPEKEGGAEI"
     gene            1469..>2373
                     /locus_tag="AC16FV_09855"
     CDS             1469..>2373
                     /locus_tag="AC16FV_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR2850395.1"
                     /translation="MKTQPTNPKQAGSTPARPPKKRAEPKSEPSPGIPGERFQIADLC
                     NILETYLEPRDVADVYRAYLFGAEAHEGQRRVSGEPYIYHPIEVARILAAMHLDAKSI
                     TAAILHDVIEDTPTAKERIAEEFGEDIAALVDGVSKITRIEFQSQEEAQAENFRKMLL
                     AMASDIRVILIKLADRLHNMRTLEAVRPDKRRRVARETLDIYAPIAQRLGMNKFKSEL
                     QDLGFRVLYPFRHRVIAARIKSMLGTRRESMGKIEAALSARLHQERIKHRIISRVKSP
                     YSIFAKMRSEHKTFAQVIDVYGFRVV"
BASE COUNT          506 a          745 c          703 g          419 t
ORIGIN      
        1 gcaaggcgaa agaattgtat cccgaagccg cgcgcttgtt tcgcgagcgg ttggaggcgc
       61 gtctgaagga aatcagacag cagctcgatc ccgcgcgcct ggaacaggag atcgtgctgt
      121 tcgcgcagag ggccgacgtg gccgaggaga tcgaccgcct cacggcacat ttcgccgaga
      181 ttcgccgcgt cttcacccag cccggccccg tcggccggcg cctcgatttc ttgatgcagg
      241 aactcaaccg cgaggccaac accctgtcgt ccaagtccac cgacacgcgc ctgaccaacg
      301 tcgcggtcga actcaaggtg ctgatcgagc agatgcgcga acaagtgcag aatatagaat
      361 aatatcttta accgcagatg aacgcagatt aaaaggagtt aacaacttta tccccatttt
      421 atctgcgttc atcattctta ctcatcagcg ttaatctgcg tttaaaaatc ttatgaacaa
      481 taaaggaaaa ctttttattt tttccgctgc ctctggtacc ggcaagacca gcctcgccaa
      541 ggcgctggtc gagagtatgc ccgacgtggc gttctcggtg tcgcacacca cgcgcgcgcc
      601 gcgcccgggc gagcagcacg gcgtgcatta ttatttcgtg acgccgcagc agttcgacga
      661 gatggtcgcg gccgaccgct ttctggaaca cgccaaggta ttcggcaatt tctacggcac
      721 ctcgcgcgcc gcgaccgaga atctgctgtg gcaaggcaag aacatcattt tcgatatcga
      781 ctggcagggc gcgcgcgcca tcaaggaaaa gatgccagag gcggtcagcg ttttcatcct
      841 gccgccctcg cgcgcggcgc tggaggagcg cctgaccggt cgcggcccgg acgcgtccga
      901 agtgatcgcg cgacgcatgc gcgaggcggt gtccgagatg agtcactata aggagttcga
      961 ccatctggtc gtgaacgacg acttcaacgc cgcgctggct gatttgaagg ccattatccg
     1021 cggggaaggc agcccccggc cggtgcgggt ggatatggag gctttgctgg cagaaggtta
     1081 gccacgggcc aagtggcaaa aaccgcccta tatcagtaag atgcgataca gcgcttatcc
     1141 ctgacccgcc cccgggactt cgtaacccgc gggcgcgtgc tttagtttta agtgtgaaac
     1201 cccgcggcaa aaccgcgttt ccttattgaa gattgaggta taggtatggc tcgtatcacg
     1261 gtagaagatt gtctggacaa cgtcgataac cgctttcagc tcgtgctggt ggccacgcgc
     1321 cgcgcgcgcc agctgatcgc cggcgcggat actttcgttc cgcgggaaaa cgacaaaccc
     1381 accgtcatcg cgctgcgcga aattgctgac ggttatgtga ccaacgccat cctggacgag
     1441 gaagcgccgc cagccggact tgaggccgat gaaaacacag ccgaccaacc ccaagcaggc
     1501 gggctcgacg ccagcccggc ccccgaaaaa gagggcggag ccgaaatctg agccgtcgcc
     1561 cgggattccc ggggagcggt tccagatcgc cgacctgtgc aacatactcg aaacttatct
     1621 cgagccccgg gacgttgctg acgtttatcg cgcctatctg ttcggcgccg aggcccatga
     1681 aggccagcgc cgcgtcagcg gcgaacccta catctaccat ccgatagagg tcgcccgcat
     1741 cctggcggcg atgcacctcg acgccaaaag catcaccgcg gcgatcctgc acgacgtcat
     1801 cgaggacaca cccacggcga aggagcgcat cgccgaggag ttcggcgagg acatcgccgc
     1861 gctcgtcgat ggcgtctcca agatcacccg catcgaattc cagtcgcagg aagaggcgca
     1921 ggcggagaat ttccgcaaga tgctgctcgc catggcgagc gacatccgcg tcatcctgat
     1981 caagctcgcc gaccgcctgc acaacatgcg cacgctcgaa gccgtgcggc ccgacaagcg
     2041 ccgccgcgtc gcgcgcgaga cgctggacat ctacgccccg atcgcgcagc ggctgggcat
     2101 gaacaagttc aagtccgaat tgcaggacct cggcttccgc gtgctctatc cgtttcgcca
     2161 ccgcgtcatc gcggcgcgca tcaagagcat gctcggcacg cgccgggaat cgatgggcaa
     2221 gatcgaggcg gcgctgtcgg cgcggctgca ccaggagcgg atcaagcacc gcatcatcag
     2281 ccgggtcaag tcgccgtaca gcatctttgc caagatgcgc agcgagcaca agaccttcgc
     2341 ccaggtcatc gacgtgtacg gattccgcgt cgt
//
LOCUS       DBNGZE010000386         3888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1928912, whole genome shotgun sequence.
ACCESSION   DBNGZE010000386 DBNGZE010000000
VERSION     DBNGZE010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1928912"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..735)
                     /locus_tag="AC16FV_09860"
     CDS             complement(49..735)
                     /locus_tag="AC16FV_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR2850396.1"
                     /translation="MIQLRKANDRGHANHGWLDSWHTFSFADYHDPAHVSFSVLRVIN
                     EDRVIPGEGFPTHPHRDMEIITYVLEGALEHKDSLGTGSVIRPGEVQRMSAGTGIRHS
                     EFNHSQSEPVHFLQIWILPNRQGVKPGYEQKRIGLDGQLRLMASPDGRDGSVTIHQDA
                     RVYAARLNGSEATHTLAPGRRAWLQVARGAVKLNETTLHAGDGAAVENESALRLTADG
                     SAEVLLFDLP"
     gene            859..1761
                     /locus_tag="AC16FV_09865"
     CDS             859..1761
                     /locus_tag="AC16FV_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR2850397.1"
                     /translation="MRLTLDALTVLDAIERKGSFAAAAEELHRVPSAMTYAVQKLEED
                     LDVLLFDRRGHRAQLTEAGRQLLSEGRHLLHAASELESRVKRVATGYEVELRIAVDDV
                     IPMERLYPLFQDFYREDCGTRLRILMEVYGGAWDALVTRRADLVIGAPAEGPSGGGYS
                     TRPLGSVEWLFAVAPSHPLARAPEPIPPEEILKHRSVAAADSSRNLPPRTSGLLSGQD
                     VLTVPDMRYKIAFQRAGFGAGYLPVHMVRDEVAAGRLVVKQVLEPKPGVQGFIAWRTA
                     DKGKALRWFLDRLEDRKFVDSLLA"
     gene            1834..2550
                     /locus_tag="AC16FV_09870"
     CDS             1834..2550
                     /locus_tag="AC16FV_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR2850398.1"
                     /translation="MFIFSRAPIVILLCLSLAACAGSTSPPDTTAGVDTERSSHSSGV
                     TYRDTPIRVIGSAPSRVSVKDDPRLGNAAAKIGIVEFSDYQCFYCRDFHQKQFTQLRK
                     EYIDTGAVQFIYKDLPLRMHAQAIPAAMAAHCAGAQNRFWDMHDALFAHQDRLGQNLY
                     PELARQLKLDEAKFNACLESQDPGQNIGQDIGTARRLGFSGTPSFLIGTIEGDTLTVK
                     YRSRGAPRFEVFAQEIEKLR"
     gene            2674..3075
                     /locus_tag="AC16FV_09875"
     CDS             2674..3075
                     /locus_tag="AC16FV_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HIR2850399.1"
                     /translation="MAEKNSGPRRPAGGLDSSKLDSIVAKARKERDTRLAGYREQSLR
                     IHPWICARCGRTFTRENLHELTVHHKDHNHDNNPSDGSNWENLCLYCHDNEHQRFAHL
                     VRGYDVGVGMEKKAAATHNPFANLKDMLKNK"
     gene            3088..3564
                     /locus_tag="AC16FV_09880"
     CDS             3088..3564
                     /locus_tag="AC16FV_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HIR2850400.1"
                     /translation="MKPVQIINRELLDRVSSLAQAAPRRRKNHNFHAAENDPCHRLLN
                     AIEPDSYIQPHCHRDPSKDETIVAVRGRVGMVFFDEQGAVTSRTTLAPAGEVVGVNVP
                     HGVYHTLVALEPNSIFFEAKAGPYAPLAPEEKAAWAPAEGDPRVAAYLTRLQQLFA"
BASE COUNT          795 a         1125 c         1193 g          775 t
ORIGIN      
        1 cgtgtaatgg ggtgatttga ttaaggccat gactgactcc tccttgattc acggtaaatc
       61 gaacaacagt acttcggctg agccatcggc ggtcaatcgc agggccgatt cattctcgac
      121 agccgcgccg tcgccggcgt gcagtgtcgt ttcattgagt ttcaccgcac cgcgcgcgac
      181 ctgcagccag gcgcgccggc cgggcgcgag cgtgtgtgtc gcttcgctgc cgttcaggcg
      241 cgcggcatag acgcgcgcgt cctgatggat ggtgaccgaa ccgtcacgac cgtcgggcga
      301 ggccatcagt cgcaactggc cgtcgagccc gatgcgcttc tgctcgtaac ctggcttcac
      361 gccctgccgg ttcggaagga tccagatttg caaaaagtgc acaggctcgc tttgggagtg
      421 attgaactcg ctgtggcgga tgccggtgcc ggcgctcatg cgctgcacct cgccggggcg
      481 gatcaccgag ccggtgccaa gactgtcctt gtgttcgagc gcgccctcga ggacgtaggt
      541 gatgatttcc atgtcgcggt gcggatgcgt cggaaaaccc tcacccggaa tgacgcggtc
      601 ttcattgatg actcgcagta cggaaaagct cacatgcgcc gggtcgtgat aatcggcgaa
      661 ggaaaacgta tgccaggagt cgagccagcc gtgattggca tggccgcggt cattggcctt
      721 acgaagctgt atcatgtcat ttcctccttt tcgccttagt ttattcacat tcgttgaata
      781 tgatggaaag aatattcttg taatttctgc gttaatagcg aaaaattccg gtcaagtcgt
      841 tcaaaaggat tgaacaacat gcgcctgacc ttggatgccc tgaccgtgct ggatgccatc
      901 gaacgcaagg gcagtttcgc ggccgccgcc gaggagctgc accgcgtgcc ctcggccatg
      961 acctatgcgg tacagaagct ggaagaggac ctggatgtct tgctatttga ccgccgcggc
     1021 caccgcgcgc aactgacgga ggcgggccgg cagctgttga gcgaggggcg tcatctgttg
     1081 cacgcggcat cggaactgga atcgcgcgtc aaacgcgtcg ccacgggtta cgaggtcgag
     1141 ttgcgcatcg cggtggacga tgtcatcccc atggagcggt tgtatccgtt gttccaggat
     1201 ttctaccgcg aggactgcgg cacgcggctg cgcatactca tggaagtgta cggcggggcg
     1261 tgggacgcac tggtgacacg gcgcgccgat ctcgtcatcg gggcgccggc tgaggggcct
     1321 tccggcggcg gctattcgac gcggccgctg ggctcggtgg agtggttgtt tgcggtggcg
     1381 ccgagccatc cgctggcacg cgcaccggag ccgatcccgc ccgaagaaat actcaagcac
     1441 cgttcggtgg cggccgccga cagctcgcgc aatctgccgc cgcgcacctc cggcctgctt
     1501 tccggtcaag acgtgctcac ggtgccggac atgcgctaca agatcgcgtt tcagcgcgcc
     1561 ggcttcggcg ccggttatct gccggtacac atggtgcgcg acgaagtggc ggcaggccgg
     1621 ctcgtggtca aacaggtgct cgaacccaaa cccggcgtgc agggatttat cgcctggcgc
     1681 acggcggaca agggcaaggc gctgcgctgg tttctcgacc ggctcgagga taggaaattc
     1741 gttgacagcc tgctggcctg attcgccgct agcgggatgg cgcaacacag acctatggct
     1801 agactgaaat gaccacccac acggatagtg accatgttta ttttctcccg cgcaccgatt
     1861 gttatcctgt tatgcctgag cctggcggcc tgcgccggtt ccacgtcgcc tcccgatacg
     1921 acagccggcg tggatacaga gcggagttca cattcctccg gcgttactta ccgggacacg
     1981 cccatccgcg tcatcgggag cgcgccgtcg cgggtgagcg tgaaggatga cccccgcctg
     2041 ggcaatgccg cggcgaagat cggcatcgtc gaattcagcg actatcagtg tttttattgc
     2101 cgtgatttcc accaaaaaca gttcacgcag ctcaggaagg aatacatcga caccggcgcg
     2161 gtgcagttca tttacaagga tttgccgctg cgcatgcatg cacaggccat tcctgctgcc
     2221 atggccgctc actgcgcggg cgcgcagaac cgtttctggg acatgcatga tgccttgttc
     2281 gcacatcagg atcgactggg acagaacctg tatccggaac tggcacgcca gctgaagctg
     2341 gacgaggcga agttcaatgc ctgtcttgaa agtcaggacc ctggccagaa tattggacag
     2401 gatatcggca cggcgcgcag gctcgggttc agcggcacgc cgtcgttttt gatcgggaca
     2461 atcgagggcg atacgctgac cgtgaagtac cgatcccgcg gcgcgccacg tttcgaggta
     2521 tttgcgcagg aaatcgaaaa gctgcggtag ttgcgggctg taccgtcacg ataggagtat
     2581 gcggggaggc ttttttaata tgacaagacg agccagcgta gcgagcaggg ctacctgtct
     2641 ataaaatacg cggactgact tggaaaaggt tttatggcgg aaaaaaattc cggtccgcgc
     2701 aggccggccg gtggattgga cagcagcaaa ctcgacagca ttgtcgccaa ggcgcgcaag
     2761 gaacgcgaca cgcgcctcgc gggttaccgt gagcagtcgc tgagaatcca tccctggatt
     2821 tgtgcgcgct gcggacgcac cttcacgcgc gaaaatttgc acgaattgac cgtgcaccac
     2881 aaggaccaca accacgacaa caacccgtcc gacggcagca actgggagaa tctctgcctc
     2941 tattgccacg acaacgagca ccagcgtttc gcgcacctgg tgcgaggtta cgatgtcggc
     3001 gtcggtatgg agaagaaggc tgcggcgacg cacaatccct ttgccaatct caaggacatg
     3061 ctgaagaaca agtaatccgt cgcccacatg aagcccgttc agatcatcaa ccgcgaactg
     3121 ctcgacaggg tttcctcgtt ggcgcaggca gccccacgtc ggcgcaagaa tcacaacttt
     3181 catgccgcgg aaaacgaccc atgccatcgc ctgctgaatg ccatcgagcc ggattcctac
     3241 atccagcctc attgccatcg cgatccatcc aaggacgaaa cgatcgtggc tgtgcgtggc
     3301 cgcgtcggca tggtgttttt cgatgagcag ggcgcggtca cttccaggac gacgctcgcg
     3361 ccggcgggcg aggtggtggg cgtgaacgtt ccgcacggcg tgtatcacac gctcgtggca
     3421 ctcgagccaa acagcatctt tttcgaagcc aaggccggtc cctatgcgcc actcgcgccg
     3481 gaggaaaagg ccgcgtgggc gccggcggaa ggcgatccac gcgtggcggc gtatctcacg
     3541 cgactgcaac aattgttcgc ttgatattca cgctgtcacg caaaccgtgg cggaataatt
     3601 tccttttttc ccgggagtga tttctatcat gactgggata tcccgaaaga cccacgattg
     3661 cggacctgtg tttgattcag gtcaaatgca ttccatgccg tatgggctaa tcaatacaat
     3721 gaaaatcgtg caactccgaa accgcaacaa gtcgaggaaa acatatgaca gcgaataaag
     3781 atgtcgtggt gtaccgggtc atcatcggac tgttgctgct caccatcgtc ggcctggtcc
     3841 tgaagttcgt ggtgctgggc cagaccggga ccggcgtaga tggaagaa
//
LOCUS       DBNGZE010000387         3925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1582524, whole genome shotgun sequence.
ACCESSION   DBNGZE010000387 DBNGZE010000000
VERSION     DBNGZE010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1582524"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="AC16FV_09885"
     CDS             complement(<1..305)
                     /locus_tag="AC16FV_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850401.1"
                     /translation="MTPVLLGIALVIAAGAFMLRYLIRSRNMQYWIGSYLRWKFSRIF
                     HRRHLRRRVYFCFVDHYEPYGGTSDKARAHERVGQWLKKYPEIADRHEDSFGKRPKHT
                     "
     gene            complement(305..1462)
                     /locus_tag="AC16FV_09890"
     CDS             complement(305..1462)
                     /locus_tag="AC16FV_09890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2850402.1"
                     /translation="MAQAIFFISAALLLYNYLAYPAGIILLARLRGGEPTPVQNTTDN
                     SSLPTVTLIIAAYNEERVIEAKIRNSLEIDYPKERFHILVVSDGSTDTTPQIVQRFQN
                     NGVQSLHEPVRRGKTAALNRAVACVTSDIVVFSDANNMFETDAVRELVKHFCDPRIGG
                     VCGLKQIYHSPERESSQGDSLYWKYESAIKESESRLGSIANADGEIFAIRRKLYQPVD
                     DTVINDDAEITFILIKKGYRVLYERRATSYEHASIRIEDDFHVKVRMVSGGFQTMARY
                     WSFLLPPRSWFAFCFISHKLLRWLAPVFMILIAVSSLVLISRPFFLGMVILQGFFYAL
                     ALVGWFARDSIGRHAVFYVPFYLCAMNLAALRGLWRFLAGTQTTQWRKAER"
     gene            complement(1484..3295)
                     /locus_tag="AC16FV_09895"
     CDS             complement(1484..3295)
                     /locus_tag="AC16FV_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR2850403.1"
                     /translation="MSTHRNQTLEELAKSYGWNDELMRLLGSLPRAVVDQYIEQAWSY
                     DQDGWRHILPPLPTSATVLCLDTRFGNTAAAFTEAGLSVTVIHPCHITTRIIRHRLDS
                     MNLPNVEVINVPSESAQLPFPDGCFDAFIHHDVVATLLANPATARSPYAMLPPPLFDE
                     VFRVLKSGSFAHFGTKNPRGYTRLLKRLRCPLGGVSGASRLASLRRLKNHARRAGFQD
                     LRVYPYMVENDRVTEVIAPTGYRSNKNSLATSERFKQIILGKTGARYLAPAYGLVCAK
                     DRHLASPLQNFIDDLASRKILAKASDEDSGFQRYLSSPGKAIITLGKAADGDENVIIV
                     IPKVSRVLTWRRKEIGIVDAVRALSPFLAARLPRLYTECSVDGVTYFAISEIPGMTVD
                     RRMRHLERLTRNAADFLIRFNQITAREIVIDDAAFENLFGAMVRQVSATYPDTRPIVE
                     RIEAYLRRAVHGRSMVTVWLHGDYKLENLIFDKESLEINGIIDWEHSRRNSLPWLDLL
                     YLLTYNRIVSEERDFFEVYRDVILPENHTSHEKSVIAAYAGAVPVTVDMRTVLACLFL
                     MHHIGFRYIYIMSREGDRHNVFTALDDIEKRLARLGA"
     gene            complement(3531..>3925)
                     /locus_tag="AC16FV_09900"
     CDS             complement(3531..>3925)
                     /locus_tag="AC16FV_09900"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR2850404.1"
                     /translation="LKMSEDLGGHVIFNNILLNDNGATGSISVPNTNFSSNNNALVGR
                     LSFDGEASIVNLNAWEAVGFDTNSFTTTSSSLFVNASTNNYRLKAGAPTINTGLPSLN
                     GITAPTTDILGTARPQGSAHDLGAYESN"
BASE COUNT          865 a         1039 c         1184 g          837 t
ORIGIN      
        1 gtatgtttcg gacgcttgcc gaagctgtcc tcatgtctgt cggcgatttc aggatatttt
       61 ttcagccatt gccccacccg ctcgtgcgcg cgcgctttgt cgcttgtgcc gccatagggc
      121 tcgtagtgat cgacgaagca gaagtagaca cgtcggcgaa ggtggcgtcg atggaatatc
      181 cgtgaaaatt tccatcgcag ataggaaccg atccagtact gcatgttgcg cgagcggatg
      241 agataccgga gcatgaatgc gccagccgcg ataacaagcg caatgccgag aaggaccggt
      301 gtcatcagcg ttccgccttg cgccattggg tggtctgcgt gccggcgaga aagcgccaca
      361 ggccgcgcaa ggccgcgaga ttcatggcac agagatagaa gggaacgtaa aaaaccgcat
      421 ggcgtccgat gctatcgcgc gcaaaccagc cgacgagcgc gagtgcatag aagaagccct
      481 gaagtatcac catgccgaga aagaatggcc tggaaatcag tacgagcgag gagacggcaa
      541 tcaggatcat gaacaccggg gcaagccagc gcagcagctt gtgcgagatg aagcagaagg
      601 cgaaccagga gcgcggcggc aacaggaacg accaatatcg ggccatggtc tgaaagccac
      661 cggagaccat gcgaaccttg acgtgaaaat cgtcctcgat gcgaatggag gcgtgctcgt
      721 atgatgtggc gcggcgttcg tacagcaccc ggtacccctt cttgatcagg ataaaggtga
      781 tttcggcgtc atcgttgatg accgtatcat cgacaggttg gtacagtttg cgccggatcg
      841 cgaaaatctc gccatcggcg ttggcgatag agcccagccg gctttctgat tccttgatgg
      901 cggactcgta cttccagtac aggctgtcgc cctgactcga ttcccgttcc ggtgaatgat
      961 agatctgctt gaggccgcag actccgccga tgcgcgggtc gcagaagtgc ttgaccagtt
     1021 cccgaactgc atctgtctcg aacatgttat tggcgtcgga gaaaaccacg atatccgacg
     1081 tcacgcatgc cacggcgcgg ttgagggcgg cggttttccc gcggcgcacc ggctcgtgca
     1141 gtgactggac gccgttattc tgaaagcgtt gcacgatctg tggcgtcgta tcagtggaac
     1201 cgtccgaaac caccagaatg tgaaatcgtt cttttggata atctatttcc agagaattgc
     1261 gaatcttggc ctcgatgacg cgttcctcgt tatacgcggc aattatcaac gtaaccgttg
     1321 gcagcgagct gttgtccgtg gtgttttgta cgggggtcgg ttcgccgccg cgaagccgcg
     1381 ccagcagaat gatgccggcg ggataggcaa ggtagttgta cagcaataac gcggcagaga
     1441 taaaaaaaat agcctgggcc atggagttac aggaattatt gtatcaggcg cctagccggg
     1501 caagacgctt ttcgatgtcg tccagcgcgg tgaatacgtt atgccggtct ccctcccggc
     1561 tcataatgta aatatagcgg aaaccgatgt gatgcatgag aaaaagacat gccaggaccg
     1621 ttctcatgtc cactgtcacc ggcacggccc cggcataggc cgctataact gacttttcgt
     1681 ggctcgtatg gttttccggc aaaatcacat cgcggtagac ctcgaagaag tcccgctcct
     1741 ccgacacaat acgattgtag gtaaggagat acagcagatc gagccatggc aggctgttcc
     1801 ggcgtgaatg ctcccagtcg atgatgccgt tgatctccag tgactccttg tcgaagatga
     1861 gattttccag tttgtagtcg ccgtgcaacc agaccgtgac catgctcctg ccatggacgg
     1921 cgcgacgcag gtaagcttcg atccgttcca cgatcggtcg tgtatccgga taagtggcgc
     1981 tgacctgcct gaccatcgcc ccgaacaggt tttcgaaggc ggcgtcatcg atgacgatct
     2041 cgcgcgcggt aatctggttg aagcggatca gaaaatccgc cgcattgcgc gtgagtcgtt
     2101 ccagatggcg catgcggcga tcaacggtca tgccgggaat ttcactgatc gcaaaatagg
     2161 taacgccgtc gacggagcat tcggtgtaca gccgcggcag ccgcgccgcg agaaacggcg
     2221 agagtgcacg cactgcatca acaataccga tctccttgcg gcgccacgtc agtacacggg
     2281 acactttggg tatgacgata atgacgtttt catcgccatc ggcagccttc cccagcgtga
     2341 taattgcttt gcccggcgac gaaagatagc gctggaaacc cgagtcttca tcggaagcct
     2401 tggcaagaat cttccgtgaa gccagatcgt ctataaagtt ctgcagcggg gaagcaagat
     2461 gacggtcctt ggcgcatacc agtccatagg ccggcgccag gtatctggcg ccggttttcc
     2521 cgaggatgat ttgtttgaat cgttcgctgg tcgccagact gtttttgttc gagcggtaac
     2581 cggtgggcgc gatgacttca gtcacccggt cattttccac catataagga taaacacgca
     2641 gatcctggaa gccggcacgt ctggcatggt ttttcaggcg ccgtaatgat gcgagccgtg
     2701 acgctcccga cacgccgccc agggggcacc gcaaccgttt caacaggcgg gtatacccgc
     2761 gaggattctt tgtcccaaaa tgcgcaaagc tgccggactt caggacgcgg aatacttcat
     2821 cgaacaacgg cggggggagc atggcgtatg ggctccgtgc cgttgccggg tttgccagca
     2881 gcgtggcgac cacatcatgg tggatgaagg catcgaagca gccatccgga aacggcaatt
     2941 gggccgactc ggacggtaca ttaatgacct ctacgttcgg gagattcatt gaatccagtc
     3001 ggtgccggat gatgcgtgtc gtgatgtgac aggggtggat cacggtcacc gaaagtcccg
     3061 cctcggtgaa agcagccgcg gtattgccga aacgggtgtc gagacatagt accgtggcgg
     3121 aggtcggaag cggagggagt atgtgtcgcc agccgtcctg gtcgtaggac caggcctgtt
     3181 cgatatattg atccaccacg gcgcgcggca agctgcccag cagccgcatc agctcatcgt
     3241 tccatccata agatttagcc agctcctcca gcgtctggtt ccgatgggtg ctcatcgaat
     3301 tttctccatg cgcggtacat atggccggga aagaaacgat tgttggtggc tggaagtgga
     3361 gtgcacgtat ctgccccaag cgcccgtcgt tcagcgcctg gatattttcg atgcggcact
     3421 ttaccagccc tgaagtgcat gcactagggc cggcggactt gatctgtaat taccacagaa
     3481 tcatgcaacc aaggggcttg gagggtttat agcgagctgt cttgacttgt tcagttggat
     3541 tcgtacgcgc ccagatcatg ggcggagcct tgcggtcgtg cggtaccgag aatgtctgtc
     3601 gtgggggccg tgatgccgtt aagggaaggc aggccagtgt taatggtcgg cgcgcccgcc
     3661 ttgagccggt agttgttcgt ggaagcattg acgaacaggc tggacgaggt ggtcgtgaac
     3721 gaatttgtgt cgaaaccgac cgcctcccag gcgttcaggt tcacaatgga agcttcccca
     3781 tcgaaggaca gacgaccgac gagggcattg ttattgctcg agaaatttgt attgggaacc
     3841 gagatgctgc ccgtggcgcc gttgtcgttc agcaggatgt tattgaagat cacgtgtccg
     3901 ccgagatcct cgctcatttt cagcg
//
LOCUS       DBNGZE010000388         5160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1929010, whole genome shotgun sequence.
ACCESSION   DBNGZE010000388 DBNGZE010000000
VERSION     DBNGZE010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1929010"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..174)
                     /locus_tag="AC16FV_09905"
     CDS             complement(16..174)
                     /locus_tag="AC16FV_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850405.1"
                     /translation="MLTAMGVPHDLGVAALRFSFGRTSEAADVEALCGALPKIVEKVR
                     SLSAVLHR"
     gene            complement(607..2019)
                     /locus_tag="AC16FV_09910"
     CDS             complement(607..2019)
                     /locus_tag="AC16FV_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR2850406.1"
                     /translation="MEIQFHGAAREVTGSCFLVRAGARQVLVDCGLIQGAPADEARNR
                     AAFAFDPRALDAVILTHAHLDHSGRLPLLVKAGFRGPVYTHRATRDLCQIMLKDAAAL
                     NEKDAEHENRRRLRQGKRPIEPLYSRQDAEKAMRQFQTLEYAVERKILPEVRLRLQDA
                     GHILGSAIVELWLEENGTRRKLVFSGDLGHAGAPILRNPEAVREADLVVLESTYGDRE
                     HRSWEETLAELGAVFREAQQSQGNILIPAFAIGRSQELLYFLKRHRREWGIDRWTVFL
                     DSPMAIEATEVYGKHWKLFDAETNQRRKKDGDPFSMPNLHFSRTAAQSMAINRVRAGA
                     IVIAGSGMCDGGRIRHHFKHNLWREQCHVIFAGFQARGTLGRKIVDGAETVKLWDETI
                     RVAARIHTIGGLSAHAGQSGLLKWYQNFERRPVVELVHGEPEPMEALARRLHLLQTHV
                     LMPKVGERIDLLAPPPKARK"
     gene            complement(2028..2909)
                     /locus_tag="AC16FV_09915"
     CDS             complement(2028..2909)
                     /locus_tag="AC16FV_09915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR2850407.1"
                     /translation="MDTIWAAIAQHIAAATGTPFTVRSRHAIGGGCINSATVLQEDGN
                     RRYFVKLNDVTRVAMFEAEAEGLKEIAQTRSVRVPVPVCSGTVDGSSYLVLEYIDLAG
                     AEARSLERLGRELAQMHRATQKQFGWRLDNTIGSTPQINTPCTDWVEFWRERRLGFQL
                     KLAVRYGHDLMRQGERLMAELEKFFRSYRPAPSLLHGDLWGGNVGATGQQPVIFDPAV
                     YYGDREADIAMTELFGGFSARFYQAYQEAWPLDPGYRQRKMLYNLYHILNHFNLFGGG
                     YGSQAERMIDSLLSELG"
     gene            3312..4811
                     /locus_tag="AC16FV_09920"
     CDS             3312..4811
                     /locus_tag="AC16FV_09920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR2850408.1"
                     /translation="MLLNQMSMRMSLTIAVSVVGLLGVFFVLKSGDVYRDASVAQQRA
                     ALGEQVKARLSDLRRNLDAESQLLALLASQEPGFAAAVAGGKTVASIRYLDNLFERPG
                     FTSGRTRLAKLQVLNTEMNVLASSGKGVPEAVLNQPACANLRSLAAKRKDTEIRKPVT
                     GVCVAEKNIYHLLIQALGERPSSGFLLVATDVSPRLTGPDNIFNLPVRLSFPDGALLF
                     RSANWPRDDEAENYLVAEQPFGIAPRALFQVAVSQDVHLFKDGLTLTGYNLVAVSASA
                     ILLAALLVWFALQKTAIKPLRTLVAQLRKTRSDKSHLGEHVVVSGCAEAVELGAAFNS
                     MTTRLKELYESLESLAFTDPLTKLPNRTLFHERLEQAIEDAKRDYKPFALMLMDLDRF
                     KDINDTLGHQVGDKLLQQVAARLRSKLRDIDTVARMGGDEFAILLPAVNDKHATMAAR
                     MLLQALRAPFRIDEQSLDIGASIGIALYPDHGVDANILIQRADVAMYAA"
     gene            4902..>5160
                     /locus_tag="AC16FV_09925"
     CDS             4902..>5160
                     /locus_tag="AC16FV_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR2850409.1"
                     /translation="MEQEQFVLYYQPKVGLKNLQITGVEALVRWNHPREDLLLPDTFI
                     PLLEQTGMIRSLTPWVLNESLRQGQQLQDQGLPITISMNLSV"
BASE COUNT          966 a         1591 c         1585 g         1018 t
ORIGIN      
        1 caatccgcaa tgcgctcacc ggtgcagcac cgccgacaac gaccgcacct tctccacgat
       61 cttcgggagc gcaccgcaca gtgcctccac gtccgccgcc tcgctggtgc ggccgaagga
      121 gaaccgcagc gccgccactc cgaggtcgtg cggcacgccc atcgccgtga gcacgtgcga
      181 cggctcgacc gaccccgtgc tgcacgccga gcccgttggc cccgagcaac aaggcgggga
      241 gtgggtattt gaatttcatt gcacacctcc tcttttggat aaatcagtta ttcgttcgtc
      301 tgctggagag acggaaacaa tcggtgaacg caaggctcgc caatgaacaa gaaactgttt
      361 caaatttttt gatcgggcac actggactcg ccgcgttcgc aaaattccct ctgccggaga
      421 ctgggcttct cctgtcaact atctaactcc tttcgttcag ggatggcaag ctgcaagcaa
      481 acagctcacg cctccttgtg ttttcaacat ccgttctgcc aatctgtcac gtcaatctgt
      541 cattgtcatg agagacgcat cgaaattttt ccgaggacag tttcatttaa agacagaatt
      601 catgctttat ttccgggctt ttggcggtgg tgccagcaag tcgatgcgct cgccaacctt
      661 tggcatcagc acgtgtgtct gtaacagatg caaacggcgg gccaacgcct ccatcggttc
      721 tggctcgccg tgcaccagct ccaccacggg acgccgttcg aaattttgat accacttaag
      781 caggcccgac tgaccggcat gcgccgacaa tccgccgatg gtatgaatgc gcgcagcgac
      841 gcggatggtc tcgtcccaga gcttgaccgt ttccgcgccg tcgacgattt tccgcccgag
      901 cgtgccacgc gcctggaaac cggcgaagat gacgtgacac tgttcgcgcc agaggttgtg
      961 cttgaagtga tggcggatgc gcccgccgtc acacataccg ctgccggcga tgacgatggc
     1021 gccggcgcgc acgcggttga tggccatgga ctgggccgcg gtgcggctga aatgcagatt
     1081 cggcatgctg aaagggtcgc cgtccttttt gcgccgctgg ttggtctcgg cgtcgaacag
     1141 cttccagtgc ttgccgtaga cctccgtggc ttcaatcgcc atcgggctgt cgagaaagac
     1201 cgtccaccga tcgatgcccc attcccgacg atgccgcttg agaaaataaa gcaactcctg
     1261 gctgcggccg atggcgaagg cggggatgag gatgttgcct tggctttgtt gcgcctcgcg
     1321 gaacaccgcg cccagttccg cgagcgtctc ttcccaagag cgatgttcgc ggtcgccata
     1381 ggtgctttcc agcaccacaa gatcggcctc gcgcacggct tccgggttgc gcaagatcgg
     1441 tgcgccggcg tgccccaggt cgccgctgaa gacgagcttg cgtcgggttc cattttcctc
     1501 cagccacagc tccacgatgg ccgagccgag gatgtggccg gcatcctgca ggcgcagccg
     1561 cacctccggc aggattttcc gttcgacggc gtattccagc gtctggaact ggcgcatcgc
     1621 cttttccgcg tcctggcgcg agtagagcgg ttcgatcgga cgcttgccct ggcgcaggcg
     1681 ccggcggttt tcgtgctcgg cgtccttctc gttcagcgcg gcggcatcct tgagcatgat
     1741 ctggcacagg tcgcgcgtgg cccggtgggt gtaaaccggc ccgcgaaagc cggccttgac
     1801 cagcaacggc aggcggccgg agtggtcgag atgggcgtgg gtgaggatca cggcgtcgag
     1861 cgcgcgcggg tcgaaggcga aggcggcgcg gttgcgcgct tcgtccgccg gcgcgccctg
     1921 gatcaggccg cagtcgacga gtacctggcg cgcaccggcg cgcaccagaa agcaggaacc
     1981 ggtgacttcc cgggcggcgc cgtggaactg gatttccatt tttttcctta tcccaattcg
     2041 ctcagcagcg aatctatcat gcgttcggcc tgggaaccgt aaccgccgcc gaaaagattg
     2101 aagtgattga gaatgtgata aaggttgtac agcatcttgc gctggcggta gccgggatcg
     2161 agcggccagg cttcctgata cgcctggtaa aagcgcgcgg aaaatccgcc gaacagttcc
     2221 gtcatggcga tgtccgcctc gcggtcgccg taatagaccg ccgggtcgaa gatcaccggt
     2281 tgttgtcccg tggcgccgac attgccgccc cacagatcgc catgcagcag tgaaggtgcg
     2341 ggccggtagc tgcggaaaaa tttttccagc tccgccatca accgctcgcc ctggcgcatc
     2401 aggtcatggc cgtagcggac ggcaagcttg agctgaaagc cgagccggcg ttcgcgccag
     2461 aattccaccc agtcggtgca gggtgtgttg atctgcggtg tcgatccgat ggtgttatcg
     2521 agacgccagc cgaactgttt ctgcgtggcg cgatgcatct gcgccagttc ccgaccgagc
     2581 cgttcgaggc tgcgcgcttc cgcgcctgcc agatcaatgt attcgagcac aaggtaggag
     2641 gaaccatcga ccgtgccgga acagaccggc actggcacgc gcacgctgcg tgtctgtgcg
     2701 atttccttca ggccttcggc ttccgcctcg aacatggcca cacgcgtcac gtcgttgagt
     2761 ttcacgaagt agcggcggtt gccgtcctcc tgcaggaccg tggcggagtt gatgcacccg
     2821 ccaccgatgg catgccgcga ccggacagta aagggagtgc cggtggcagc ggcaatgtgc
     2881 tgtgcgatcg cggcccagat ggtatccatt ctcccgcctc cccctccgag accggtaatg
     2941 tagggccaat cccgggaggc gagaagcccg ttttggtcca gcgccgcgcc ggaaagtggc
     3001 accggaagga ccgaaaggca gccagacccc gccattgatc ccgccgttgg caaataatcg
     3061 ccaaaatcgc cttgttttca gccattattt tccatggccc gctgatttta tggatgccgg
     3121 catatggcac tagatttgca agatttaccc gccgccaacg gatttgggcc aggggatggt
     3181 ttgccccggg cggcgtaggg atggggaaat ccagggaggt gatcagggtt ccgatggaag
     3241 gggaacgaca acgaagggat tcgcatcgac gtttattcgt cttcagtcgg ttttggcgac
     3301 taagaataac gatgctgctg aatcagatgt ccatgcgcat gagcctcacc atcgccgtgt
     3361 cggtggtggg attgcttggc gtgttttttg tgctgaagag cggcgacgtc taccgcgacg
     3421 ccagcgtggc gcagcagcgc gccgcgctcg gcgagcaggt caaggcacgt ctgagcgatc
     3481 tgcgccggaa tctggacgcg gaatcgcagc tgttggccct gttggccagt caggagcccg
     3541 gatttgccgc cgccgtggcg ggtggcaaaa cggtggcctc gatccgttat ctggataatc
     3601 tgtttgaacg ccccggtttc acctcaggcc gcactaggct ggccaagctc caggtgctga
     3661 atacggaaat gaatgtattg gcctcgtccg gcaagggcgt gcccgaggcg gttttgaatc
     3721 aacccgcttg cgccaatctg cgctccttgg cggccaagcg caaagacacc gaaatccgaa
     3781 agcctgtcac cggcgtctgt gtggcggaaa aaaatattta ccatcttctg atacaggcgt
     3841 tgggtgaacg gccttcatcc ggttttctgc tggttgcgac cgatgtgtca ccgcggctga
     3901 ccggccccga caacatcttc aatctgcccg tgcgtctgtc tttcccggac ggagccctgc
     3961 tgttccgctc cgccaattgg ccgcgtgacg atgaagccga gaattatctg gtggcggaac
     4021 agcccttcgg tatcgcgccg cgcgcgctgt ttcaagtcgc cgtcagccag gatgtccacc
     4081 tgttcaagga tggattgacg ctcaccggct acaatctcgt ggccgtgtct gcctcggcga
     4141 ttctgctggc cgcgctgctg gtatggtttg ccttacagaa gaccgccatc aagccgctgc
     4201 gcacgctggt cgcccagctg cgcaagaccc gttcggacaa aagccatctc ggcgagcatg
     4261 tggtggtgag cggttgcgcc gaagccgtcg agctgggcgc cgccttcaac agcatgacga
     4321 cgcggctcaa ggagctgtat gaaagcctgg aaagcctggc cttcaccgac ccgttgacga
     4381 aactgccgaa ccgcacgctg ttccacgaac gcctggagca ggcgatcgag gacgccaagc
     4441 gcgattacaa gcccttcgcc ctgatgctga tggatctcga tcgtttcaag gacatcaacg
     4501 acaccctggg tcaccaggtc ggtgacaagc tcttgcaaca ggttgccgcg cgcctgcgca
     4561 gcaagctgcg cgacatcgac acggtggcgc gcatgggcgg cgacgagttc gcgatcctgt
     4621 tgccggcggt gaatgacaaa cacgcgacca tggcggcgcg catgctgctc caggccttgc
     4681 gcgccccgtt ccgcatcgac gagcagagcc tcgacatcgg cgccagcatc ggcatcgcgc
     4741 tctaccccga tcacggcgtg gacgccaata tcctgatcca gcgcgcggac gtggcgatgt
     4801 acgccgccta gaacagcaac ggtggacacg ccttctacga cggcaagatc gaccagcaca
     4861 acccgacacg cctgacgcta ctcggcgagc tgcgccatgc cgtggagcag gagcagttcg
     4921 tgctgtatta ccagccgaag gtgggtctca agaatctcca gatcacgggc gtcgaggcac
     4981 tggtacgctg gaatcacccg cgcgaggacc tgttgctgcc ggataccttt atcccgttgc
     5041 tggagcagac cggcatgatc cgcagcctca cgccgtgggt gctgaacgaa tccctgcgtc
     5101 agggccagca gcttcaggat cagggactgc cgatcaccat ttccatgaac ctgtcggtgc
//
LOCUS       DBNGZE010000389         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_677861, whole genome shotgun sequence.
ACCESSION   DBNGZE010000389 DBNGZE010000000
VERSION     DBNGZE010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_677861"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="AC16FV_09930"
     CDS             complement(<1..637)
                     /locus_tag="AC16FV_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR2850410.1"
                     /translation="MHLTLRQLKVFESVARHLNYTRAAEELFLTQPAVSMQVKQLEES
                     LGVALFEQLGKRIHLTEAGQEVLGYARSVTQQLDELESVLNRIKGLSGGRLRISVATT
                     ANYFIPTLLGTFSRRYPEVTVSLDVTNREALLRQLSENTVDLVIMGQPPSGLDVEAEA
                     FMENPLVIVAPPGHPLAQQKKIPLKRLQEEVFLVREPGSGTRSAMERFFAER"
     gene            804..2231
                     /locus_tag="AC16FV_09935"
     CDS             804..2231
                     /locus_tag="AC16FV_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HIR2850411.1"
                     /translation="MSGKVKTYKAGVKEYRETYWMPNYKPSDTDLLACFKITPQAGVP
                     REEAAAAVAAESSTGTWTTVWTDLLTDLDYYKGRAYRIEDVPGDDTCFYAFIAYPIDL
                     FEEGSVVNVFTSLVGNVFGFKAVRALRLEDVRFPIAYVKTCGGPPQGIQMERDIMNKY
                     GRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRARFDFVME
                     AIHKAEKETGERKGHYLNVTAPTPEEMYKRAEYAKEIGAPIIMHDYITGGFCANTGLA
                     NWCRDNGMLLHIHRAMHAVLDRNPHHGIHFRVLTKILRLSGGDHLHTGTVVGKLEGDR
                     EATLGWIDLLREKYIKEDRSRGIFFDQDWGSMPGAFAVASGGIHVWHMPALVTIFGDD
                     SVLQFGGGTLGHPWGNAAGAAANRVALEACVEARNQGIAIEKEGKTVLEKAAKSSPEL
                     KIAMETWKEIKFEFDTVDKLDVKHK"
     gene            2297..2653
                     /locus_tag="AC16FV_09940"
     CDS             2297..2653
                     /locus_tag="AC16FV_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO small subunit-like domain-containing
                     protein"
                     /protein_id="HIR2850412.1"
                     /translation="MSEMQDYKSSLTDTASRKFETFSYLPAMDVANIKKQVEYLVKKG
                     WNPAIEHTEPENAFDHYWYMWKLPLFGETNVDKVLAEADACHKAHPNNHVRLVGYDNF
                     KQSQGAAMVIYRGKMK"
     gene            2738..>3051
                     /locus_tag="AC16FV_09945"
     CDS             2738..>3051
                     /locus_tag="AC16FV_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2850413.1"
                     /translation="MSKTSTATKVDNGADIAQYLVKQEPYYRPVSNEVELYEAAYSAR
                     MPVMLKGPTGCGKSRFVEYMAWKLKKPLITVACNEDMTASDLVGRFLLDASGTKWHDG
                     PLT"
BASE COUNT          668 a          936 c          936 g          511 t
ORIGIN      
        1 cgcgctcggc gaaaaagcgc tccatggcgc tgcgcgtgcc cgagcccggc tcgcgcacga
       61 gaaacacctc ttcctgcaat cgtttcagcg gaattttttt ctgctgcgcc agcggatgcc
      121 ccggcggcgc cacgatgacc agcgggtttt ccatgaaggc ctcggcctcg acgtcgagac
      181 ccgacggcgg ctggcccatg atgacgaggt ccacggtatt ctccgaaagc tgccgcagca
      241 gcgcctcgcg gttggtgacg tcgaggctga ccgtcacctc cggatagcgg cgcgagaaag
      301 tgccgagcag agtggggata aagtagttgg ctgtggtcgc gaccgagatg cgcaacctcc
      361 cgccggacag tcccttgatg cggttcagca ccgattccag ctcgtccagt tgctgggtca
      421 cgctgcgcgc atagccaagc acctcctggc cggcctcggt caggtggatg cgcttgccca
      481 gttgctcgaa cagcgccacc ccgaggctct cctccagttg tttgacctgc atggaaacgg
      541 ccggctgggt gaggaaaagc tcttccgccg cgcgggtata gttcagatgg cgcgcgaccg
      601 attcgaagac cttgagttgg cgcagcgtga ggtgcaaagt atcggccccg atataactat
      661 aagtaataat ttatcataat aatcataaaa tgtgagtgtt atttatgata cggggatagt
      721 agggttagcg cccaagatgc aggtcgcccg ggaaaaatgg gcggaatacc ggaattcaaa
      781 cgatttctaa cgaggagtcc cttatgtcag gcaaagtgaa gacgtacaaa gcgggcgtca
      841 aagagtaccg cgaaacctac tggatgccga actacaagcc gtcggacacc gatctgctgg
      901 cgtgcttcaa gatcacgccg caggccggcg tgccgcgcga agaggccgcc gccgcggtgg
      961 ccgccgagtc gtccaccggc acctggacca cggtctggac cgacctgctg accgatctcg
     1021 actactacaa aggccgcgcc taccgcatcg aagacgtgcc cggcgacgac acctgtttct
     1081 acgccttcat cgcctacccg atcgacctgt tcgaagaggg ttcggtggtg aatgtgttca
     1141 cctcgctggt cggcaacgtg ttcggcttca aggccgtgcg cgcgctgcgc ctcgaagacg
     1201 tgcgcttccc gatcgcctac gtgaagacct gcggcggacc accgcagggc atccagatgg
     1261 aacgcgacat catgaacaag tacggccgcc cgctgctcgg ctgcaccatc aagccgaagc
     1321 tcggcctctc cgccaagaac tacggccgcg ccgtgtacga gtgcctgcgc ggcggtctcg
     1381 acttcaccaa ggacgacgag aacgtcaaca gccagccgtt catgcgctgg cgcgcgcgct
     1441 tcgacttcgt gatggaagcg atccacaagg cggagaagga aaccggcgag cgcaagggcc
     1501 actacctgaa cgttaccgcg ccgaccccgg aagagatgta caagcgcgcc gagtacgcca
     1561 aggaaatcgg cgcgccgatc atcatgcacg actacatcac cggcggcttc tgcgccaaca
     1621 ccggcctggc caactggtgc cgcgacaacg gcatgctgct gcacatccat cgcgccatgc
     1681 acgccgtgct cgatcgcaac ccgcaccacg gcatccactt ccgcgtgctg accaagatcc
     1741 tgcgcctgtc gggcggcgat cacctgcaca ccggcacggt ggtcggcaag ctcgaaggcg
     1801 accgtgaagc gacgcttggc tggatcgacc tgctgcgcga gaagtacatc aaggaagacc
     1861 gcagccgcgg tattttcttc gatcaggact ggggttccat gcccggtgcg ttcgccgtgg
     1921 cctccggcgg tatccacgtc tggcacatgc cggcgctggt cacgatcttc ggtgacgact
     1981 cggtgttgca gttcggcggc ggcacgctcg gccatccgtg gggcaacgcc gccggcgccg
     2041 ctgccaaccg cgtggcgctg gaagcctgcg tcgaagcacg caaccagggc atcgccatcg
     2101 agaaggaagg caagactgtt ctcgagaagg ctgccaagtc cagtcccgaa ctcaagatcg
     2161 ccatggaaac atggaaagag atcaagttcg agttcgacac cgtcgacaag ctggacgtga
     2221 agcacaagta aaccaaggcg atcggccggg cggctaagtc gcccggccaa cctgatctga
     2281 tactgaggaa tagacaatga gtgaaatgca agactacaaa tccagcttga ctgacaccgc
     2341 cagccgcaag ttcgagacct tctcgtacct gccggcgatg gacgtggcca atatcaagaa
     2401 gcaggtggag tatctggtga agaagggctg gaacccggcc atcgagcaca ccgagccgga
     2461 aaacgccttc gatcactact ggtacatgtg gaagctgcca ctgttcggcg agaccaacgt
     2521 ggacaaggtg ctggccgagg ccgatgcctg tcacaaggcg catccgaaca accacgtgcg
     2581 cctggtcggt tacgataact tcaagcagtc gcagggcgcc gccatggtga tctaccgcgg
     2641 caagatgaag taactcgtac cccaagcaag ggtaacaacg aagcccctgc tcgggtaacc
     2701 gggcaggggc ttttgtttag cgattccgag gcaagcaatg agcaaaacga gcacagcaac
     2761 gaaggtcgat aacggcgcgg atatcgccca gtacctggta aaacaggagc cgtattaccg
     2821 gccggtgtcg aacgaggtgg agttgtacga agccgcctac agcgcgcgca tgccggtgat
     2881 gctgaagggc ccgaccggct gcggcaagag ccgcttcgtc gagtacatgg cgtggaagct
     2941 caagaaaccg ctgatcacgg tggcgtgcaa cgaggacatg accgcgtcgg acctggtggg
     3001 ccgtttcctg ctcgatgcca gcggcaccaa gtggcacgac ggcccactca c
//
LOCUS       DBNGZE010000390         2364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1721455, whole genome shotgun sequence.
ACCESSION   DBNGZE010000390 DBNGZE010000000
VERSION     DBNGZE010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1721455"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>2364
                     /locus_tag="AC16FV_09950"
     CDS             175..>2364
                     /locus_tag="AC16FV_09950"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR2850414.1"
                     /translation="MSKSYDPHAIETRIYETWERAHYFSAAGQGAPYCIMIPPPNVTG
                     SLHMGHAFQDTLMDTLIRYHRMQGDNTLWQAGTDHAGIATQMVVERQLEAEGKTRHDL
                     GREEFIKRVWQWKAESGGTITRQLRRMGASLDWSRERFTMDERLSHAVTEVFVRLHEE
                     GLIYRGKRLVNWDPVLHTAVSDLEVVSEEEAGHLWHIRYPIAGTQEYLVVATTRPETM
                     LGDAAVAVHPEDERYKKFVGKMVELPLTGRKIPVIADEYVDREFGSGCVKITPAHDFN
                     DYEVGKRHNLPLINIFTSDAHIKESSLDDFTWGHRAPDKRGALLADGDGLTGKATVDL
                     IPKKYHGLDRFKARKQIVTDLEEQGFIERVQDHKFMVPRGDRTGAVIEPFLTDQWYVK
                     VGPLAQEAIRCVEDGRIKFVPENWTKTYYEWMRNIQDWCISRQIWWGHRIPAWYDADG
                     NVYVARNAAEAHAQARHQRGQDVPLEQDEDVLDTWFSSALWPFSTLGWPEQTKELKTF
                     YPTSVLVTGFDIIFFWVARMIMMGLKFTSEVPFREVYIHGLVRDAHGQKMSKSKGNIL
                     DPIDLIDGIDLESLVAKRTKGLMQPQMAKKIEQATRKEFPDGIPAYGTDALRFTFASL
                     ATQGRDIKFDLGRIDGYRNFCNKLWNAARYVLMNTEGHDCGLSGKIELNAADRWIVSR
                     QQEVAAEVEAAFKEYRFDLAAQAIYSFIWHEYCDWYLELSKVVLTDTH"
BASE COUNT          560 a          705 c          662 g          437 t
ORIGIN      
        1 caacaattga tacgtcagga actggagaaa ggcggggaaa aatgaagcga ccggtaaaaa
       61 gtgaaaaaat accgataact cactagaatt ccgcccgctt tttcaacttt taccgtttcg
      121 atgagccaga acccacaaaa acccgctggc ggcggcccgg cgtccgaaag caccctgagc
      181 aagtcctacg atccgcacgc gatcgaaacg cggatttatg agacttggga acgcgcacac
      241 tatttctccg cggccggtca gggcgcgccc tactgcatca tgatcccgcc gcccaacgtc
      301 acgggtagcc tgcacatggg ccatgcgttc caggacacgc tcatggacac gctcatccgt
      361 taccaccgca tgcagggcga caacaccctg tggcaggcgg gcaccgacca cgccggcatc
      421 gccacccaaa tggtggtcga gcgccagctc gaggccgagg gcaaaacccg ccacgacctc
      481 gggcgcgagg aattcatcaa gcgcgtgtgg caatggaagg ccgaatccgg cggcaccatc
      541 acccgccagc tgcggcgcat gggcgcctcg ctcgactggt cgcgcgaacg cttcaccatg
      601 gatgaaagat tgtcacacgc cgtcaccgaa gttttcgtgc gcctgcacga ggaaggtttg
      661 atttatcgcg gcaagcgctt ggtcaactgg gacccggtgc tgcacaccgc ggtatccgac
      721 ctcgaggtcg tttccgagga agaagccggc cacctctggc atatccgtta tccgatcgct
      781 ggcacccagg aatatctggt ggtcgcgacg acacgccctg aaaccatgct gggcgacgcc
      841 gccgtggcgg tacatccgga agacgagcgt tacaaaaaat tcgtcggcaa aatggtcgag
      901 ctcccgctca ccggccgcaa gattccggtg attgcggatg aatacgtgga cagggaattc
      961 ggctccggct gcgtcaagat cacgccggcg catgacttta acgactacga agtcggcaag
     1021 cgccacaatc tgccgctcat aaacattttc acgtccgacg cccacattaa agaaagttct
     1081 cttgatgatt tcacttgggg tcacagagcc ccagacaaac gtggtgctct gcttgctgat
     1141 ggtgatggac taaccggaaa agcaactgtc gacttaattc caaaaaaata tcatggatta
     1201 gatcgtttca aggcacgtaa gcagattgtc actgatcttg aagaacaagg gtttattgag
     1261 agagtgcaag atcacaaatt catggtcccg cgcggcgacc gcaccggcgc cgtgatcgag
     1321 ccttttctca ccgaccagtg gtacgtgaag gtcggcccgc tggcgcaaga ggccatccga
     1381 tgcgtggaag acggccgcat caagtttgtc cccgaaaact ggaccaagac ctattacgaa
     1441 tggatgcgca acatccagga ctggtgcatc tcgcgccaga tctggtgggg ccaccgcatc
     1501 ccggcctggt acgacgccga cggcaacgtc tacgtcgcgc gcaatgccgc cgaggcgcat
     1561 gcccaagcca ggcaccagcg cggtcaggat gtcccgctgg aacaggacga ggacgtgctc
     1621 gatacctggt tttcctcggc gctgtggccg ttctccacgc tcggctggcc ggaacaaaca
     1681 aaagagctga aaaccttcta tcccacgagc gtgctcgtca ccggcttcga catcattttc
     1741 ttctgggtcg cgcgcatgat catgatgggg ctgaaattta ccagcgaggt gccgttccgc
     1801 gaggtctata tccacggctt ggtgcgcgac gcccacggcc agaagatgtc gaaatccaag
     1861 ggaaatattc tcgacccgat cgatctcatc gacggcatcg atctggaatc gctcgttgcc
     1921 aaacgtacga aaggcctgat gcaaccgcag atggcgaaga aaatcgaaca agccacgcgc
     1981 aaggaatttc ccgacggcat tcccgcctat ggcactgatg cgctgcgctt taccttcgcc
     2041 agccttgcga cacaagggcg cgacatcaaa ttcgacctcg ggcgcatcga cggctatcgc
     2101 aacttctgca acaagctatg gaacgccgcg cgctacgtgt tgatgaacac cgaggggcac
     2161 gactgtggat taagtgggaa aatcgaactg aatgccgccg accgctggat tgtttcgcgc
     2221 cagcaggaag tcgcggccga ggtggaagcc gcgttcaagg aataccgttt cgatcttgcc
     2281 gcacaggcaa tctacagttt catctggcac gagtactgcg actggtacct ggaactctcc
     2341 aaggtcgtgc tgacggatac ccac
//
LOCUS       DBNGZE010000391         1759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1091414, whole genome shotgun sequence.
ACCESSION   DBNGZE010000391 DBNGZE010000000
VERSION     DBNGZE010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1091414"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16FV_09955"
     CDS             <1..189
                     /locus_tag="AC16FV_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850415.1"
                     /translation="RSQVDAMAARTELTMPGENVLAHTAHMYQPRTRRPYGSLEWPAM
                     LRRLDAEQKNTDYPPYWH"
     gene            216..1187
                     /locus_tag="AC16FV_09960"
     CDS             216..1187
                     /locus_tag="AC16FV_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR2850416.1"
                     /translation="MATTLLTRLIMAAALLFALTPGAFADDYPNRPVRLIVPFPPGGS
                     NDVVGRLVAMQLSTQLGQQVFVDNRSGAGGVIGTEACATAAPDGYTLCIISIAHAVNP
                     ALHKLNYDPIKSFTPISIFATGPNVLAINPSLPVNSVKELVALAKQKPGELNYASAGV
                     GSFQHLGGELFKLTAGVNIVHVPYKGGGPAMQDVISGHVKIMFSSLIQTTPFIKSGQL
                     KALGTGGAKRSAVLPDVPTIGEAGVPGYVADNWWGLAAPAGLPAPLVEKLYAASQAAL
                     KASELQAQFEREGATTVVMTTPEFGEYIKTEIAKWARVVKEGNIHAQ"
     gene            1484..>1759
                     /locus_tag="AC16FV_09965"
     CDS             1484..>1759
                     /locus_tag="AC16FV_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIR2850417.1"
                     /translation="MKGAIALALMLAFGAAAPVQAAEGQIAVVAAENFYGDIARQIGG
                     DRVAVVSIMNNPDQDPHLFETTPSIVRQLAAAQIVIINGANYDPWMDK"
BASE COUNT          372 a          517 c          514 g          356 t
ORIGIN      
        1 cgctcgcagg tggatgcgat ggccgcacgc accgaactga ccatgcccgg cgagaacgtg
       61 ctggcgcata cagcgcatat gtatcaaccg agaacgcggc ggccatacgg tagtctcgaa
      121 tggccggcca tgctgcgccg gctcgacgcc gagcagaaga acaccgatta cccgccttac
      181 tggcactaaa caaatttccg atatagggag gccccatggc gacgacactt ctcactcgat
      241 taatcatggc cgccgcgctg ctgttcgcgc ttacgccggg cgccttcgcc gacgactacc
      301 cgaaccggcc agtacggtta attgttccgt tcccgcccgg cggcagcaac gatgtggtcg
      361 gtcgcctggt cgccatgcaa ctgagtacac aacttggcca gcaggtattc gtcgacaacc
      421 gcagcggcgc cggcggcgtg atcggcaccg aggcttgtgc aaccgcggcc ccggacggct
      481 acacgctctg catcatatcg atcgcacacg cggtcaatcc ggccttacat aaactcaact
      541 acgatccaat caaatcgttt acgccaatct cgatttttgc gaccgggccg aatgtgcttg
      601 cgatcaatcc gagcctgccg gtcaattctg tgaaggagct ggtggcactc gctaaacaga
      661 aacccggtga actcaattat gcctcggcag gcgtcggcag ctttcaacat ttgggcggcg
      721 aactgttcaa gctcacagcc ggcgtcaaca tcgtgcacgt gccatataag ggcggcggtc
      781 cagcgatgca ggacgtgatc agtggccatg tgaaaatcat gttctcatcg ctgatccaaa
      841 ccacgccttt catcaagtcg ggccagctca aggcgctggg cactggtggc gccaagcgca
      901 gtgcggttct tccggatgtg ccgacgattg gggaggcggg cgtgcccggc tatgtggcag
      961 acaactggtg ggggctggcc gcccctgcag gactacccgc gccgctcgtc gaaaaactgt
     1021 atgcggcgag ccaggcggcg ttgaaggcgt cggaactgca agcacagttc gagcgtgaag
     1081 gtgcaactac agtcgtgatg accacgcccg agtttggcga gtacataaaa accgaaattg
     1141 ccaaatgggc ccgcgtggta aaggagggca atattcacgc gcaatagaaa atattacgcg
     1201 tttgcgcgta tagccgctct tctatggctc atgaatgttt ggccgtcagc gcgattctga
     1261 aggcgcccct atgatttgtc cgccaccgat atcgcgcgcg agcagatttc cccctcggta
     1321 ccgaccgcac gccgtatgat gcgattttgg ctcggtcgcc gcatgccgaa ggtcgacgtc
     1381 taaaccttgg gtgaatgctc ggcacatctg gccgttgccg tttgcaatgt tataatgtta
     1441 cattttgagg ggtaagcttt ctgctgccag gggaaatcga tttatgaaag gcgccattgc
     1501 tctcgccctt atgctggctt ttggtgcggc cgccccagtc caggcggccg agggtcaaat
     1561 tgcggttgtg gcggcggaga atttctacgg cgatatcgcc cggcagattg gcggcgaccg
     1621 agttgcggtc gtcagcatca tgaacaaccc ggaccaagac ccgcatctgt tcgagaccac
     1681 gccaagtatc gtgcgtcaac ttgcggccgc gcagatcgtt atcatcaatg gcgccaatta
     1741 cgatccctgg atggacaaa
//
LOCUS       DBNGZE010000392         2826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1165836, whole genome shotgun sequence.
ACCESSION   DBNGZE010000392 DBNGZE010000000
VERSION     DBNGZE010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1165836"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..264)
                     /locus_tag="AC16FV_09970"
                     /pseudo
     CDS             complement(19..264)
                     /locus_tag="AC16FV_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(366..2576)
                     /locus_tag="AC16FV_09975"
     CDS             complement(366..2576)
                     /locus_tag="AC16FV_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR2850418.1"
                     /translation="MADMKVAAYICKGCGIGERLDTAQLAMIAQKEGKAQVVREHEFL
                     CNASGVEMIKNDIANEGVNHVVIAACSRRAKAETFNFENVAISRANLREGVIWITPAD
                     DDKKELVQEMAADYVRMGCAESAKMQIPRTNPNVGHNKRILVVGGGISGMTAAIEASK
                     TGYDVLLVEKTGSLGGWAAKLHKRVPYREPYADPMDTGIAGLIKQIESDQHIKVHLNS
                     TITKTSGAPGQFSVDISTESGGTTTENIGGIIMASGFTPYDMNKLPHLGAGKSKDVVD
                     QLGLEALAKAANGGPIKRPSDGKEVKSVVFIQCAGQRSDKEGELPYCSGHCCNTSIKQ
                     AMYFKDQNPNVDTVVMYTDLRTPGNGEDFYRSAQKKGVIFTKGVANEVVPGSELQVKF
                     QDKILNEEAVAKADLVVLATGQVANSGLDIEQVPQDEPGKWAANKQSVLNMVYRQGKD
                     LPLLKHGFNDSHFICFPYETRRTGIYTAGPVRRPMDMVQAIEDATGAALKAIQAVENA
                     GLGRAAHPRSGDLSFPEFRKEGCTQCKRCTVECPFGAIDEDEQRYPVFNESRCRRCGT
                     CMGACPVRVISFENYSVDTVGYQLKAVDVPDQFSEKPRILVLACENDAYPALDMAAMN
                     HIQYSAFTRVIPVRCLGSVNTIWVTDALNSGYDGVILMGCQKGDNYQCHFVKGSELAH
                     VRMSKIDDTLKQLSLEIERVKTYEVAITDIQRAPALINDMAETIQKIGLSPLKF"
     gene            complement(2582..>2826)
                     /locus_tag="AC16FV_09980"
     CDS             complement(2582..>2826)
                     /locus_tag="AC16FV_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase subunit A"
                     /protein_id="HIR2850419.1"
                     /translation="NPHDLVVLAIGMEPSVDASKIMPGVAADASGFIDINATTEAVFG
                     AGCASNALDVNRSVQSATAGALRAIQVINRVSRAEA"
BASE COUNT          455 a          883 c          882 g          606 t
ORIGIN      
        1 cgggcagcgc acgattcagt tgtagcagct ggtgcacatc cacatggact cggagcgcaa
       61 tacctcgtcg cgcttgccgg cgcggatcat catgaagatc tcctggggcg ggtgctccca
      121 ggacgggccg agcgggcagg agccggagca cacgccgcac tgcatgcaca tcttgaccca
      181 gttgccctcc tcgacgttcg cctcgacttc cttgaggaag tcgttgcggt acttctccac
      241 cattgcggta tttaaatctg tcattacggg ttcctcgttt gaacctgttc gatatatcta
      301 tttcttccta ctccccctct cccgcaagcg ggagagggta aggggagagg gcgacaagca
      361 ctgcgttaaa acttcaaggg gctgagtccg atcttctgga tcgtctcggc catgtcgttg
      421 atcagcgccg gcgcgcgctg gatgtcggtg atcgcgacct cgtaggtctt cacgcgctcg
      481 atctcgagcg agagctgctt cagcgtgtcg tcgatcttgc tcatgcggac atgcgccagt
      541 tccgagccct tcacgaagtg gcactggtaa ttatcgccct tctggcaacc catgaggatc
      601 acgccgtcat aaccgctgtt gagcgcgtcg gtgacccaga tggtgttgac cgaaccgagg
      661 cagcgcaccg ggatcacgcg ggtgaaggcg ctgtactgga tgtggttcat ggccgccatg
      721 tcgagcgccg ggtaggcgtc gttctcgcag gccagcacca ggatgcgcgg cttctcggag
      781 aactgatccg ggacgtccac tgccttgagc tggtaaccca cggtgtccac cgaatagttc
      841 tcgaacgaga tcacgcgcac cgggcaggcg cccatacagg tgccgcagcg gcggcagcgg
      901 gattcgttga acaccgggta acgctgttcg tcctcgtcga tggcgccgaa cgggcattcc
      961 acggtgcagc gcttgcactg ggtgcagcct tccttgcgga actcggggaa cgacaggtcg
     1021 ccggagcgtg gatgcgccgc gcggccaagc ccggcgtttt ccaccgcctg gatcgctttc
     1081 agcgcggcgc cggtggcgtc ctcgatcgcc tgcaccatgt ccatcgggcg gcgcaccggg
     1141 ccggcggtgt agatgccggt gcggcgcgtt tcgtacggga aacagatgaa gtgcgaatcg
     1201 ttgaagccgt gcttcagcag cggcaggtcc ttgccctggc gatagaccat gttcaacacc
     1261 gactgcttgt tggcggccca cttgcctggc tcgtcctgcg gcacctgctc gatgtccagt
     1321 ccggaattgg ctacctggcc agtcgccagc accacgaggt cggccttggc gactgcttcc
     1381 tcgttcagaa tcttgtcctg gaacttcacc tgcaattcgg aaccgggaac aacttcgttg
     1441 gccacgccct tggtgaagat cacgcccttc ttctgggcgc tgcggtagaa gtcctcgccg
     1501 ttgcccggcg tgcgcaggtc ggtgtacatc acgaccgtgt cgacgttcgg gttctggtcc
     1561 ttgaaataca tcgcctgctt gatcgaggtg ttgcagcagt ggccggagca gtagggcagc
     1621 tcgccttcct tgtcggagcg ctggccggcg cactggatga acaccacgct tttcacttcc
     1681 ttgccatcgg acggccgttt gatcggcccg ccgttggcgg ccttggccaa ggcttccagt
     1741 ccgagctgat cgaccacgtc cttgctcttg ccggcgccga ggtgcggcag cttgttcatg
     1801 tcatagggcg tgaaaccgga ggccatgatg atgcccccga tgttttcggt cgtggtgcca
     1861 ccggattcag tggagatgtc cacgctgaac tggcccggtg cgccgctggt cttggtgatg
     1921 gtcgagttga ggtgcacctt gatgtgctgg tccgactcga tctgcttgat caggccggca
     1981 atgccagtat ccatcggatc ggcgtagggc tcgcgatacg gcacgcgctt gtgcagcttg
     2041 gccgcccagc cgccgaggct gccggtcttt tccaccagca acacgtcgta accggtcttg
     2101 gaggcctcga tcgccgcggt catgccggaa atgccgccgc cgaccaccag gatgcgtttg
     2161 ttgtggccga cgttgggatt ggtgcgcggg atctgcatct tggccgattc ggcgcagccc
     2221 atgcgcacgt aatccgcggc catttcctgc accagctcct tcttgtcgtc gtccgccggc
     2281 gtgatccaga tcacgccttc gcgcaggttg gcgcgcgaga tggcgacgtt ctcgaaattg
     2341 aaggtctcgg ccttggcgcg acgcgagcag gctgcgatca ccacgtgatt cacaccctcg
     2401 ttggcgatgt cgttcttgat catctccacg ccgctggcgt tgcacaggaa ttcgtgttcg
     2461 cgcacgactt gggccttgcc ttccttctgc gcgatcatcg caagctgtgc cgtgtcgagg
     2521 cgctcgccga tgccgcagcc cttgcagatg taagcagcaa ctttcatgtc cgccatgggt
     2581 attaagcctc cgccctggat acgcggttaa tcacttgaat cgcccgcagc gctccggccg
     2641 tggcgctttg caccgagcgg ttgacgtcga gcgcgttgga cgcacaaccg gcaccgaaca
     2701 ccgcctcggt ggtcgcattg atgtcgatga agccgctggc atccgccgcc acgcccggca
     2761 tgatcttgga cgcgtcgacc gacggctcca tgccgatcgc gagcactacc aggtcatgcg
     2821 ggttgt
//
LOCUS       DBNGZE010000393         9578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1166010, whole genome shotgun sequence.
ACCESSION   DBNGZE010000393 DBNGZE010000000
VERSION     DBNGZE010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1166010"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="AC16FV_09985"
     CDS             complement(<1..509)
                     /locus_tag="AC16FV_09985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2850420.1"
                     /translation="MLHFIANGLVVAAIILQLLALRPVRRTIALLSPGRLRFSWYVLT
                     AFIFIFIIGYSAYIPAFWGTHEQFADLVVPAVFFLGACFVLVVNLLSLETARNVKRLR
                     AFEQESITDPLTGIYNRRYLERRLVDEIARSSRYGMPLSVLLVDIDHFKRVNDIYGHQ
                     VGDLALEGMA"
     gene            complement(606..1196)
                     /locus_tag="AC16FV_09990"
     CDS             complement(606..1196)
                     /locus_tag="AC16FV_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HIR2850421.1"
                     /translation="MKQHSPSCERNKDPILTVLREIFTVPGLVLEIGSGSGQHAVHFA
                     RGLPHMEWQPTDIAENLPGISAWRAEAGLPNLREPLVLDLFADGWPVREAQTLVCINT
                     IHIVSWPGVERLFAGAGRVLAPGGIMYVYGPYRYAGRPLEPSNEDFDRWLKARDPVSG
                     VRLFQDVNHLAGQHGLVLAGDRAMPANNRSIWWQKS"
     gene            1251..1595
                     /locus_tag="AC16FV_09995"
     CDS             1251..1595
                     /locus_tag="AC16FV_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HIR2850422.1"
                     /translation="MPYLKIQTNRETADDAKQEILKKASVLVSKNLGKPEKYVMVRID
                     PAQPMMFAGTAAPCAYLELKSIGLPESKTEALSKVLCQLMLDELKIPSERVYIEFADA
                     KDSMWGWNSGTF"
     gene            complement(1829..2950)
                     /locus_tag="AC16FV_10000"
     CDS             complement(1829..2950)
                     /locus_tag="AC16FV_10000"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR2850423.1"
                     /translation="MHAVTKAQPVPFLDLSAQYKALESEWLAAIRETGSRGSFILGPH
                     TQAFEKEFAEYVGVKHAIAVANGTDSLYLSLRALGVGKGDEVITTPFTFFASAETIDM
                     VGATPVFVDILPDSFCLDPASVRAKITSKTKAIVPVHIFGHPSEMGEIMEIAKKHKLA
                     VIEDCAQSFGALHNGKVVGSIGDTGSFSFYPTKVLGCYGDGGMVTTNSDAVNEHIRRL
                     RNHGAVKPFLHAEIGMNSRLDEVQAAALRIKLRHIKEDIAGRQKVAAEYTRRFASSPV
                     KTPSLPKNGTHVFNLYTIRVPKRDAVRQVLTDAQIGTSQCYPQGLHLQEVYKHLNYKP
                     GSLPVCEHACTETLSLPVYPGMPLDHVERVSEVLLAALK"
     gene            complement(2938..3918)
                     /locus_tag="AC16FV_10005"
     CDS             complement(2938..3918)
                     /locus_tag="AC16FV_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR2850424.1"
                     /translation="MTAAAHTQATGNAGKLRVGVAGVGYLGRFHARIYAAMPDVELVG
                     VADSNERVAKEVAEQHGCRAYTDARDLLGNVDAVSIVVPTIYHGDVARPFLEKGVHML
                     MEKPIAPTYEESRALVELAERAGVIFQVGHLERFNAGIMALAERAQNPRFIEVHRLGP
                     FVERATDVDVVTDLMIHDIDIVLSLVKSSIRNIAATGIPVLTEHVDIANARIEFENGA
                     VANVTASRVSNKKLRRIRVFGADHYYGLDYIDQKLEMVRSEPDPQGGKWPRIVTEQLD
                     ITPRPPLDAELEYFVKSVRTGTPPLVTGRVGLEALRVALLVKEKIAACTP"
     gene            complement(4032..5945)
                     /locus_tag="AC16FV_10010"
     CDS             complement(4032..5945)
                     /locus_tag="AC16FV_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR2850425.1"
                     /translation="MPDIRPALLVTQEAVLDEEILQSLVPAGRTRRFKTLAEIRKDIE
                     RSIVIASPDQSREMLSRRKAEATSFPKFSIVLWLDGPVQEIDRALLDHPQVAGALDAR
                     CPRGTVYTTLKSALALLQKSGERRAAQMLEHVLEIGRALASEKDLDSLLGLILTHARN
                     LTGADGASVYTRDANGKLYFRLWQNASTGATSNAQKSLVGDYSIAGYVAREGRPVILE
                     DAYALPLDAPYQFNPASDRSIGYRTKSLLTVPLKNKADEVVGVLQLINRKENPETVLR
                     WPEDFENSVQPFDEQDSAIALALAGQAGVALENSLLYADIERLFEGFIMASVQAIEAR
                     DPTTAGHSFRVADFTERTAIAADRADAPGLREVTFSKEQLRELRYAALLHDFGKVGVR
                     ENVLVKAKKLQPQHLELLKQRFRYARASIERRAYRRLLELHNERLKEYELTTRRREIE
                     KELAADQARLEQYLQHVLRANEPTVSHEQVSTELGEVVTFRFPGEEGEELPLLHDFEF
                     ADLALSKGSLNAEERAQIESHVSHTYAFLSLIPWTRNLAQLPEIAFAHHEKLDGSGYP
                     RRLAADKIPVQSRIMTIADIYDALTAGDRPYKPGMPEEKALDILAAEARAAKIDAALY
                     KVFVESGAWRLKH"
     gene            6032..6874
                     /locus_tag="AC16FV_10015"
     CDS             6032..6874
                     /locus_tag="AC16FV_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2850426.1"
                     /translation="MESKSVLITGCSTGIGNCLARGLRERGYRVFASARKETDVAALS
                     AAGFESLPLDLDSSGSIRDAVETVLIRTRGRLYALINNGAYGQPGAVEDLSRAVLRAQ
                     FETNLFGTQELTNLVIPAMRAQGEGRIIQMSSMLGIVCLAYRGAYNATKFALEALSDT
                     LRLELRDTNIYVSIIEPGPIESRFRANAYEAFKRNIDREHSVYREYYARVEKRLEGTK
                     PLPFTLPPEAVLKKVIRALEARRPKARYGVTFPTYLFAVLRRVLPVRVLDQILSATSN
                     GGKR"
     gene            complement(6946..7248)
                     /locus_tag="AC16FV_10020"
     CDS             complement(6946..7248)
                     /locus_tag="AC16FV_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850427.1"
                     /translation="MKVPPEVKRRWEARFPPLDGKWTHWKITGTGEFIALVDGLDLIG
                     ALKIVEENYGKYITIKASRATVNENGELAEEEKALFEISRLEYDEMTRVPDDKSDT"
     gene            complement(7325..7972)
                     /locus_tag="AC16FV_10025"
     CDS             complement(7325..7972)
                     /locus_tag="AC16FV_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850428.1"
                     /translation="MLKYLKNIIAILGSLPALVFAAEPVDLSADTYKANLGVLIIQVN
                     WGRAWKCGQFENAQLQALTFTKSPVDTPKPVSLDLETPSKLLVDNKFLPYAFVIQPGE
                     YFLTAFDVKVARSVSDVAHIKGSKDTLIKDSKPVGGTFTVNPGEIVYVGHFGLDCGAE
                     PFLWRNYIEGRADFERYIDGFREKFPFVKHVPVQYRLSSTQLFGNPYTLKDPTVK"
     gene            complement(8079..8564)
                     /locus_tag="AC16FV_10030"
     CDS             complement(8079..8564)
                     /locus_tag="AC16FV_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2850429.1"
                     /translation="MKKQAKNTICLWYDRDAEDAARFYAKTFPDSSVGAVHLAPGDYP
                     SGKKGDVLMVEFTVMGIPCLGFNGGPEVKHNWAFSFQVATTDQAETDRYWNAIVGNGG
                     EESECGWCKDKWGLSWQITPLALTEGVTDPDRAAATRVFDAMMQMRKIDIAAIEAARR
                     G"
     gene            complement(8653..9231)
                     /locus_tag="AC16FV_10035"
     CDS             complement(8653..9231)
                     /locus_tag="AC16FV_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850430.1"
                     /translation="MREPHHSEQLPPEYFEALGRLLVSFQALEEAVTLGVAHLTGSDR
                     SDEVNFKYFLALSELPFRSRVKLLRNFLETTEPSHYLWKGCPAEETRQKGIPGIISQM
                     ISFAKECNSLEDQRNQMVHSVWEPGSGEVGAVAMRFKLRVQDKGLNIAHEEVPRQKIL
                     DLVDRMQAIRNGLEKGTTLLADLLAEKRANAL"
     gene            complement(9358..>9578)
                     /locus_tag="AC16FV_10040"
     CDS             complement(9358..>9578)
                     /locus_tag="AC16FV_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR2850431.1"
                     /translation="SSKLVQLLLKNDLVDELWLKIFPLTLGTGKKLFGDSTIPAAFTL
                     TKNVITQTGVIVAYYKRAGEVRTGTIGA"
BASE COUNT         1956 a         2823 c         2743 g         2056 t
ORIGIN      
        1 gccatgcctt ccagcgccag gtcaccgacc tgatgcccgt agatgtcgtt gacgcgcttg
       61 aaatgatcga tgtcgaccag cagcacggaa agcggcatgc cgtagcggct agagcgcgcg
      121 atttcatcca ccaatcggcg ttccagataa cggcggttgt agatgcccgt cagaggatcg
      181 gtgatgctct cctgctcgaa ggctctcagg cgtttcacat tgcgcgcggt ttcgagcgac
      241 aggagattga ccaccaacac gaagcatgcg cccagaaaaa acacggcggg cacgaccaga
      301 tcggcgaact gttcgtgggt gccccagaat gccggaatgt aagcactgta cccgatgatg
      361 aaaataaaaa taaaggcagt cagcacatac cagctgaacc gcagacggcc gggggaaagc
      421 agtgcgatcg tgcgccggac gggcctgagc gccagtaact ggagaattat cgcggcaacc
      481 accaatccgt tggcaataaa gtgcagcatt atggcgaaag cacctgaaaa gcgttaccgg
      541 gcccggttca aagtatcatc tgctgagcgc cgtgtgaagc cgatcagaaa agcaagctgg
      601 ctgcgtcaac tcttttgcca ccatatcgaa cggttgttcg ccggcatggc gcgatcgccg
      661 gcgagcacaa gtccgtgctg cccggccaga tggttcacat cctggaacag gcgcactccg
      721 gataccgggt cacgcgcttt cagccagcgg tcgaaatcct cgttgctcgg ttcgagcggc
      781 cggccggcat aacggtaggg cccatagaca tacatgattc ctcccggcgc gagcacccga
      841 ccggcgccgg cgaataatct ttccacgcct ggccatgaaa cgatgtgtat ggtgttgatg
      901 cacaccagcg tctgcgcttc ccgcaccggc caaccatccg cgaacagatc cagcaccaat
      961 ggctcgcgta gattgggcaa cccggcctcc gcgcgccacg cggaaatgcc tggaaggttt
     1021 tcagcgatat cggtcggctg ccattccatg tgcggcagac cgcgcgcgaa atgcaccgcg
     1081 tgctggccgc tgccactgcc gatttccagc accaggccgg ggacggtaaa aatctcgcgc
     1141 agcaccgtga ggatcgggtc cttgttgcgc tcacaggagg gggaatgctg tttcatggat
     1201 ggcgctatgg tggattatta ttcctgacag gacaaccaag gaggatcgcc atgccctatc
     1261 ttaaaatcca gactaaccgc gagacagcgg acgatgcaaa acaggaaatc ctgaaaaagg
     1321 cctcggtgct ggtatccaag aacctcggca aacctgaaaa atacgtcatg gtgcgtatcg
     1381 atcccgccca acccatgatg ttcgccggaa ccgccgcgcc ctgtgcctat ctcgagctca
     1441 aatccatcgg cctgccggag agcaagaccg aagcgttatc gaaagtgttg tgccagctga
     1501 tgctggacga gctcaaaatc ccgtcggagc gggtttatat cgaattcgcg gacgcgaagg
     1561 attccatgtg gggatggaac agcgggacgt tctgaatcat gacgcgttcc ccggcgatcc
     1621 ggagggagcc cgaggcgggt tcactctcgc aggggcatgc cgcgcgcgtc cgacggccgc
     1681 cgccagcagc gccgtgccga acagcgccag caccgcctgc ccgatcccga tagccagcgg
     1741 gccgctcggc tatagacgcc cagcgcactt tcaaatcacg cgcaagatat gagcatgcgt
     1801 tgaatccact ccgggggtac cgaccgcttt atttgagcgc ggcgagcaga acttcactta
     1861 cacgctcgac gtgatcgagc ggcatgccgg gataaaccgg caacgaaagg gtttcggtgc
     1921 aggcgtgttc acacaccggc aggctgccgg gcttgtaatt aaggtgcttg tacacctcct
     1981 gaagatgcaa accctgcgga taacactgcg aggtgccgat ctgcgcatcg gtcagaacct
     2041 ggcgcacggc gtcgcgtttg gggacgcgga tggtgtagag attgaacaca tgcgtgccgt
     2101 tcttcggcag cgaaggcgtc ttcaccggtg agctcgcgaa acgcctggtg tattccgccg
     2161 ccaccttctg ccggccggcg atgtcttcct tgatatgccg cagcttgatg cgcagcgccg
     2221 cggcctgaac ctcgtcgaga cggctgttca tgccgatctc ggcgtgcaga aagggcttca
     2281 ccgcgccgtg gttgcgcaag cggcggatat gttcattgac cgcgtccgag ttggtcgtga
     2341 ccatgccgcc atcgccgtag caaccgagga ccttcgttgg ataaaaactg aaactgccgg
     2401 tgtcgccgat ggagccgacc accttgccgt tatgcagcgc gccgaaggac tgggcgcagt
     2461 cctcgatcac ggctagtttg tgttttttcg cgatctccat gatttcgccc atctcggacg
     2521 ggtgaccgaa aatatgtacc ggaacgatgg ctttggtttt ggatgtgatc ttcgcgcgca
     2581 cgctcgcggg gtcgaggcag aagctgtccg gcagaatgtc cacgaacact ggtgtggcgc
     2641 cgaccatgtc gatggtttca gccgaggcaa aaaaggtgaa tggcgtggta atgacctcat
     2701 cgcccttgcc tacgccgagc gcccgcaacg acaggtaaag cgaatccgtg ccgtttgcca
     2761 ctgcgatggc gtgcttcacg ccgacgtatt cggcgaattc cttttcgaaa gcctgcgtat
     2821 gcgggccaag aatgaaactg ccgcggctgc cggtctcgcg gatggcggcg agccattcac
     2881 tttcaagggc cttgtactgc gcggaaagat cgagaaacgg gacgggttgt gccttggtta
     2941 cggcgtgcat gcggcaattt tctccttgac gagcagggcc acgcgcaacg cctcgagccc
     3001 caccctgccg gtgacgaggg gcggcgtgcc ggtgcgcacg gatttcacga aatattcgag
     3061 ttccgcgtcc agcggcgggc gcggcgtgat gtcgagctgc tcggtgacga tgcgcggcca
     3121 cttcccgccc tgcgggtcgg gttcggagcg caccatttcg agcttctggt cgatgtagtc
     3181 gagaccgtaa taatgatccg cgccgaacac gcggatgcgg cgcagcttct tgttcgagac
     3241 gcggctggcg gtaacgttcg cgaccgcgcc gttctcgaat tcgatacggg cgttggcaat
     3301 gtccacgtgc tcggtgagca cgggaatgcc ggtcgcggcg atgttgcgta tgctcgactt
     3361 caccagcgac agcacgatgt cgatgtcgtg gatcatgagg tcggtcacca cgtccacgtc
     3421 ggtggcgcgc tcgacgaacg gaccgaggcg gtgcacctcg atgaaacgcg gattctgcgc
     3481 gcgctcggcc agcgccatga tgccggcgtt gaagcgttca agatgtccca cctggaaaat
     3541 caccccggca cgctcggcca gttccaccag cgcacgcgat tcctcataag tcggcgcgat
     3601 gggtttctcc atcagcatat gcacgccctt ttcgagaaac ggtcgcgcca cgtcgccgtg
     3661 gtaaatggtg ggcaccacga tactcacggc gtccacgttg ccgagcaggt cgcgcgcatc
     3721 ggtgtaagcg cggcagccgt gctgctcggc cacttccttc gccacgcgct cgttcgaatc
     3781 ggcgacgccg accagttcga catccggcat ggcggcgtag atgcgtgcat gaaagcggcc
     3841 gagatagccg acgccggcga cgccgacacg cagctttccg gcgtttcctg tcgcttgtgt
     3901 gtgggcagcc gcggtcatgg gttgttgggg tggcggagtc cttcagccag ggcggatata
     3961 aaaaacaata gtgtgtttgc cgcgcggatt ataccgaaat tcggcacctg cgccacgaat
     4021 cggcgccgtg gttaatgttt tagccgccac gcaccggatt cgacaaaaac tttatacagt
     4081 gccgcatcga tcttggcggc cctggcctca gccgcgagga tgtccagcgc cttttcctcc
     4141 ggcatgccgg gtttgtacgg ccggtcgcct gccgtaagag cgtcgtaaat atcggcgatg
     4201 gtcatgatgc gcgactgcac cgggatttta tcggccgcga ggcggcgcgg ataaccgctg
     4261 ccatcgagtt tctcgtgatg ggcaaaggcg atttccggca gctgcgccag gttcctggtc
     4321 cacggtatca ggctcaggaa tgcgtaggta tgagagacat gcgactcgat ctgggcgcgc
     4381 tcctcggcat tgaggctgcc cttggacagg gccaggtcgg cgaattcaaa atcatgcagc
     4441 agcggcagtt cctcgccctc ctcccccggg aaacggaaag tcaccacctc tcccagttca
     4501 gtggaaacct gctcgtgcga cacggtgggc tcgttcgcgc gcaacacatg ctggagatat
     4561 tgctccagcc gcgcctggtc ggcagccagc tccttctcga tctcgcgccg gcgcgtggtc
     4621 agttcgtatt ccttcaggcg ttcgttgtgc aactccagca atcttcgata ggcgcgccgc
     4681 tcgatgctgg cgcgtgcata acggaaacgc tgcttgagca attcgagatg ttgcggctgt
     4741 agctttttgg ccttgaccag cacgttctcg cgcacgccga ccttgccgaa atcgtgcagc
     4801 aacgcggcgt agcgcagctc gcgcaactgt tcttttgaaa aagttacttc ccgcaagccc
     4861 ggtgcatcgg cgcggtccgc cgcaatcgcc gtgcgttcgg tgaagtcggc cacgcgaaag
     4921 gaatgccccg ccgtggtcgg gtcgcgcgcc tcgatggcct gcaccgaggc catgatgaaa
     4981 ccttcgaaca ggcgttcgat gtcggcatac agcaggctgt tctccaatgc cacgccggcc
     5041 tgtcccgcca gcgccagcgc gatggcgcta tcctgttcat cgaacggctg cacgctgttt
     5101 tcgaaatctt ccggccagcg cagcacggtt tcgggatttt ccttgcggtt gatgagctgc
     5161 agcacgccga ccacttcatc cgccttgttc ttgagcggga cggtcaacag cgatttggtg
     5221 cgatagccga tggaacggtc cgaagcaggg ttgaactgat acggcgcatc gagtggcaac
     5281 gcgtaggcgt cttccagaat gaccggcctg ccttcgcgcg cgacataacc ggcgatgctg
     5341 taatcgccca ccagcgactt ctgggcattg gaggtagcgc cggtggaggc gttttgccac
     5401 agacgaaaat acagtttgcc gttggcgtcg cgcgtgtaca ccgatgcgcc gtcggcgccg
     5461 gtcaggttgc gcgcgtgcgt caggatcaaa cccagcaggc tgtcgaggtc tttttccgaa
     5521 gcgagcgcgc gcccgatttc cagcacatgt tcgagcattt gcgccgctcg tcgctcgccg
     5581 cttttttgca acagagccag cgccgacttg agcgtggtgt aaaccgttcc gcgcggacaa
     5641 cgcgcatcca acgcgcccgc cacctgtgga tgatccagca aggcgcggtc aatctcctgg
     5701 accgggccgt ccagccacag gacgatggaa aatttcggga atgaagtcgc ttccgccttg
     5761 cgccgggaca acatctcgcg ggattgatcc ggcgaggcaa tgacaatgga tctctcgata
     5821 tccttgcgga tctctgcgag cgtcttgaaa cggcgtgtac gtccagccgg cacaagggac
     5881 tgtaatatct cctcgtcgag aaccgcttcc tgtgtcacca acaaagcagg acgaatatcg
     5941 ggcatgcgct ggatacgtta ttgaactggg gcgttaaaca ccctacagtc actttccacc
     6001 cggtgcaacc cgcagaaccg acgacacttg tatggaatca aaaagcgtat tgatcacagg
     6061 ctgttccacc ggcatcggca actgcctcgc gcgcggcctg cgggagcgcg gctaccgggt
     6121 gttcgccagc gcgcgcaagg aaaccgatgt cgcggccctg tcggcggcgg gctttgagag
     6181 cctgccgctc gatctggact cctccggttc gattcgtgac gccgtggaaa cggtgctcat
     6241 ccgcacccgt ggccggcttt acgccctgat caacaacggc gcctacggcc agccgggcgc
     6301 ggtcgaggat ttgtcgcgag cggtattgcg ggcgcaattc gagaccaacc tgttcggcac
     6361 acaggaactg acgaacctcg tcatccccgc catgcgcgcc caaggcgagg gccggatcat
     6421 ccagatgagt tccatgctcg gcatcgtgtg cctcgcctat cgcggcgcct acaacgccac
     6481 caagtttgcg ctcgaagccc tgtccgacac cctgcgcctg gaactgcgcg ataccaatat
     6541 atatgtctcc atcatcgaac ccggcccgat cgaaagccgc ttccgcgcca acgcctacga
     6601 agccttcaag cgcaatatcg accgcgaaca cagcgtgtat cgtgaatatt acgcgcgcgt
     6661 ggaaaagcgc ctcgaaggca ccaagcccct gccctttacg ttgcccccgg aagccgtgtt
     6721 gaagaaagtc attcgcgcgc tggaggcgcg ccggccgaag gcgcgctatg gcgtgacttt
     6781 tccgacgtat ctttttgccg tgctgcggcg ggtgctgccg gtgcgagtgc tcgaccaaat
     6841 cctgtccgcc accagcaatg gggggaagcg ataacgattc aaataagcgg cgcgcggttt
     6901 tgcgcgtccg gcccgcgcct ttcgcgggcg aacttgattt gattgttagg tatcagattt
     6961 atcatctggc accctggtca tttcatcata ttcaagccga gaaatttcaa acagggcctt
     7021 ttcctcttca gccagctctc cattctcatt taccgtagcg cgactggctt tgatggtgat
     7081 gtactttccg taattctcct ctactatttt caatgcacca atcaaatcca gaccgtctac
     7141 gagcgctata aattcgcctg ttccggtaat tttccagtga gtccatttcc catccagcgg
     7201 cggaaatcga gcttcccatc ttcttttgac ttctggaggt actttcatcg cgttattgat
     7261 acctaacgat aagcttgaac ggcgcgccgc ttttgcgcgt ccgcctcgaa gcgtttgttg
     7321 ggcctcattt gacagtggga tctttaagag tatatggatt gccaaaaagc tgtgtcgaag
     7381 agagcctgta ttgaactggc acatgtttca caaacggaaa cttctctctg aaaccatcaa
     7441 tatatctctc aaagtcagcc cgaccctcta tgtaattccg ccagagaaaa ggttctgccc
     7501 cgcaatcgag accgaaatgt cctacgtaaa cgatttctcc gggatttacc gtaaatgtac
     7561 cacccaccgg cttactatct tttatcaacg tgtccttcga tccctttata tgggctacat
     7621 cgcttactga acgtgcaacc tttacgtcaa aggcggttaa gaaatattcg cctggttgaa
     7681 tgacgaacgc atagggcaag aacttgttgt ccactaacaa tttggatggc gtttcaaggt
     7741 caagagacac gggctttggt gtatcaaccg gggatttggt gaatgtgagt gcttgaagct
     7801 gtgcgttctc gaactgaccg catttccacg cacggcccca gttcacttgg ataatgagaa
     7861 ctccaagatt ggccttgtaa gtatctgcgc taaggtccac aggttctgcc gcaaagacaa
     7921 gcgccggcaa cgacccaaga atcgctatta tgtttttcag atatttaagc attcggtttc
     7981 tcctgtgagg cccaacgtat tgagttcagc ggcgcgcccg cagggtgcgt ccgctgcaac
     8041 gattggttag ccaccaccgg atgctcgaac tacctgcgtc aaccacggcg cgccgcctcg
     8101 attgccgcaa tgtcgatctt cctcatctgc atcatcgcat cgaacacacg cgtggcagca
     8161 gcgcgatcgg gatcggtcac cccttctgtc aaagccagtg gggtaatctg ccaggagagg
     8221 ccccatttgt ctttgcacca gccgcactca ctctcttcgc caccgttgcc cacaatcgcg
     8281 ttccaataac gatctgtttc ggcctggtca gtggttgcga cctgaaacga gaatgcccaa
     8341 ttatgcttga cctcgggccc gccattgaat ccgaggcagg ggattcccat aacggtaaac
     8401 tcgaccatca acacatcccc tttcttccct gacggatagt cccccggtgc gaggtgcacc
     8461 gcaccaacgg atgaatcggg aaaagtcttg gcgtaaaatc gcgccgcgtc ctcggcgtcg
     8521 cggtcgtacc aaaggcaaat tgtgtttttt gcttgcttct tcatgtcata cctccctgcg
     8581 cacgctggct aacgtttgac atgaggggcg gccaagggcg ctagctcttg gatgtccctc
     8641 tcgatgaaag ggttagagcg catttgcacg cttttcggcc aggaggtccg cgagcagcgt
     8701 cgtccctttt tccaggccat tccgaattgc ttgcattcga tctactaggt ccagaatctt
     8761 ttggcgtgga acttcttcgt gtgcaatgtt cagtcctttg tcttgcactc gaagtttgaa
     8821 tctcatcgct actgctccga cttccccact tccaggctcc caaacggaat gcaccatctg
     8881 gttgcgttga tcttctagag agttgcactc cttggcgaag gagatcatct gggaaatgat
     8941 cccgggaatg cctttttgcc ttgtctcctc ggcggggcag cctttccata ggtagtggga
     9001 gggctctgtt gtctcaagaa agttccgcag tagtttgact ctgcttcgaa acggaagctc
     9061 gcttagagca aggaagtact tgaaattgac ttcatctgac ctgtctgatc cggttagatg
     9121 agcgacccct agagtcacag cttcctccaa tgcttgaaaa gagacgagca agcgccccag
     9181 ggcttcgaag tactcgggcg gaagttgctc ggaatggtgt ggttctcgca tgcgctctaa
     9241 cgttcgccat aaccggcgtg caagagcaga gcgaagcggc gcttttgcac gtccgagttg
     9301 atggcgttgt tagccatgac ttattttgtt ctcaggcagt aaaccatagc tctcgtctca
     9361 agctccaata gtacctgtcc tgacttcgcc agctcgcttg tagtaggcaa caatcacacc
     9421 ggtttgcgta attacattct tcgtcaacgt aaatgctgct gggatggtgc tatcgccaaa
     9481 caatttcttt cctgtgccaa gcgtcaaagg gaaaattttg agccagagtt cgtccactaa
     9541 atcattcttg agtaacagct gaacgagctt actgctgc
//
LOCUS       DBNGZE010000394         1688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1513762, whole genome shotgun sequence.
ACCESSION   DBNGZE010000394 DBNGZE010000000
VERSION     DBNGZE010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1513762"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1094
                     /locus_tag="AC16FV_10045"
     CDS             144..1094
                     /locus_tag="AC16FV_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2850432.1"
                     /translation="MRDRIQLVFSLGIFALVALLPAIVRNEYWQGVLIVSMYFAMLAA
                     AWNLLAGYTGQFSLAPAAFGMIGAYVTGLLSYHFNAPPLVGIPASVVVAAAIGFGLGR
                     IVLRLRGPYLALTTLSFAEIFRLVISNSIEITRGDLGLNVPGLFDHRLTWYYVMLGVL
                     VVIQLGLYFLLRAPAGLYLRAIRDDEIAAASRGVKTVLWKTNAFTLSAAISGLAGALY
                     GHFAQFVSPELGLLSQTGIIVSMVVIGGLGTLPGPIGGAFLVYVASEFLRDFGGYQLI
                     VFALLVIIFGRFFREGLWGFFRQAIERTARRPAPATSAAE"
     gene            1101..>1688
                     /locus_tag="AC16FV_10050"
     CDS             1101..>1688
                     /locus_tag="AC16FV_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2850433.1"
                     /translation="MACLSIDRVSKFFGGLKALDNVTFSVNEGELVGLIGPNGSGKTT
                     LLNILSGYLSADSGDVVLHGRPITGLRPEALARLGVLRMFQLTRVFPRMSVLDNLLVT
                     GSALGLPEAEAEGRARELIEDLTLTRMMHLDAGQLSGGQRKLLEFGSCFMVPPRIALL
                     DEPFAAVHPTMKEIMADVIRRRHAAGQTFLLVSHDM"
BASE COUNT          249 a          556 c          493 g          390 t
ORIGIN      
        1 ttcggcagcg tttacatctc ctaccaatat cgcgacacct tcggtctggt gatcctcatc
       61 atggtcttgc tgttccggcc acagggcctg ttcggcgagc ggagccgcga ggtttgatgg
      121 ctttaacctt cagcgatact cgcatgcgtg accgaatcca gctcgtgttc tcacttggga
      181 tattcgcgct ggtcgcgctg ctgcccgcga tcgtgcgcaa cgaatattgg cagggtgtcc
      241 tcatcgtctc gatgtatttc gcgatgctgg cggcggcgtg gaatttactg gccggctaca
      301 cgggacaatt ctcgcttgca ccggcagcat tcggcatgat cggcgcctat gtgactggct
      361 tgctctccta tcatttcaac gcgccgccgc tcgttggcat tccggcgtcc gtagtggttg
      421 cagcggcgat cgggttcggc cttggccgca tcgtccttcg actgcgcggc ccctatcttg
      481 cattgaccac gctgtcgttc gccgagatct tccgcctcgt gatcagcaat tcaatcgaga
      541 tcacgcgcgg cgacctcggc cttaacgtgc cggggctctt cgaccaccgg ctcacttggt
      601 attatgtgat gctcggcgtg ctcgttgtca tccagctcgg gctctatttc cttctgcgcg
      661 cacccgcagg cctttatctg cgcgccattc gcgacgatga gatcgctgcc gcgagccgcg
      721 gcgtgaagac cgtgctgtgg aagaccaacg cctttacgct cagcgccgcc atcagtgggc
      781 ttgccggcgc gctctacggg catttcgcac agttcgtcag tcccgaactc ggcctccttt
      841 cgcagaccgg cattatcgtc agcatggtgg taatcggcgg cctcggcacc ctccccggcc
      901 cgatcggcgg cgccttcctg gtctatgtcg cctccgagtt cttgcgcgac ttcggcggct
      961 atcagctcat cgtgttcgcg ctgcttgtca tcattttcgg ccgcttcttc cgcgaaggct
     1021 tgtggggttt cttccgccaa gccatcgaac gcaccgcgcg ccgaccggcg cccgctacct
     1081 ccgcggcgga gtgaggcgcc atggcctgcc tttccatcga tcgcgtgagc aaattcttcg
     1141 gcggcctcaa ggcactcgac aacgtgactt tctccgtgaa cgagggtgag cttgtcggcc
     1201 tcattggccc gaacgggtcc ggcaagacga cgctgctcaa tatcctgagc ggttatctct
     1261 ctgctgattc tggtgacgtt gttctccacg gccgcccgat caccgggctg cggcctgagg
     1321 cgctggcacg gctcggcgtg ctgcgtatgt tccaactcac gcgcgttttc ccgcgcatga
     1381 gtgtgctcga caatctgctg gtcaccggca gtgcgctggg gctgcccgaa gccgaggccg
     1441 aagggcgcgc gcgcgaactc atcgaagatc tgacacttac acgcatgatg catctcgacg
     1501 ccggtcagtt gtcgggcggt cagcgcaagc tgctcgagtt cggctcctgt ttcatggttc
     1561 caccgcgcat cgcgctactc gacgaaccgt tcgcggcggt ccacccgacc atgaaggaga
     1621 tcatggcgga cgtcattcgc cgtcggcacg ccgccggcca aaccttcctg ctcgtcagtc
     1681 acgacatg
//
LOCUS       DBNGZE010000395        11132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_402551, whole genome shotgun sequence.
ACCESSION   DBNGZE010000395 DBNGZE010000000
VERSION     DBNGZE010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_402551"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1369)
                     /gene="argJ"
                     /locus_tag="AC16FV_10055"
     CDS             complement(149..1369)
                     /gene="argJ"
                     /locus_tag="AC16FV_10055"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR2850434.1"
                     /translation="MAVGLHGLPVLHPVPGIRLGTTAAGIRKKDRRDLVVIECTPGTQ
                     ASATFTQNRFCAAPVTVAREHLAKRAPRALLINTGFANAGTGEPGMEDARACCEALAS
                     GMNCRATEILPFSTGVIGERLPRERLLSGLPSCLANLDANGWTEAAHGIMTTDTLPKG
                     SSRQIKIDSRTITVTGIAKGAGMIRPDMATMLAFVATDAAVAPAVLHACVTTAVNQSF
                     NRITVDGDTSTNDACVLLATGAAGNPVIERAEGNVYAALLGAVTEVFMELAQAIVRDA
                     EGATKFITIEVRGGRSESDCLAVAYTIAHSPLVKTAFFASDPNWGRILAAIGRAPIAQ
                     LDVARVTIHLNELCVVHNGAPGAGYTEAKGLAVMRLPEIRIGVELGAGTASARVWTSD
                     LSHDYVRINAEYRS"
     gene            complement(1373..4081)
                     /gene="secA"
                     /locus_tag="AC16FV_10060"
     CDS             complement(1373..4081)
                     /gene="secA"
                     /locus_tag="AC16FV_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR2850435.1"
                     /translation="MITKALTKIFGSRNTRLIKRMQQDVARINALETGIAQLTDEQLR
                     AKTAEFRTRLSNGDTLDGILHEAFAVVREASKRTLGMRHFDVQLIGGMVLHQGKISEM
                     RTGEGKTLVATLPVYLNALSGKGVHVVTVNDYLARRDAEWMGQIYKFLGMSVGVVVPG
                     QDRQTKREAYAADITYGTNNEYGFDYLRDNMAFRAEERAQRGLNYAIVDEVDSILIDE
                     ARTPLIISGPAEESTDLYVKVNTIIPHLTKQETENGPGDYSVDEKSRQAYLTEAGHER
                     VEKLLAQAGLLAAGSSLYDVSNLILMHHLIAALRAHALFKREVDYIVRDNEVIIIDEF
                     TGRMMQGRRWSDGLHQAVEAKEGVAIQNENQTLATITFQNYFRLYGKLAGMTGTADTE
                     AFEFQQIYGLEVVVIPTHRPMVRQDMADQIYRTAAEKHAAILVDIRDCHQRGQPVLVG
                     TVSIDASEHLSRLLQKEKIEHQVLNAKHHEREAMIVAQAGRPGALTIATNMAGRGTDI
                     VLGGNLEMELKEIGDDKAKREHARAEWEKRHQQVIAAGGLHMIGTERHESRRIDNQLR
                     GRSGRQGDPGSSRFYLSLEDNLLRIFGSDRLSGLMQKLGMQEGEAIEHPWVTRAIENA
                     QRKVEGRNFDIRKQLLEYDDVANDQRKVIYEQRNRLMDVEDISESITTIRHDVVNELI
                     SQHVPPESLEEQWDISGLEESLERELGLNLPIAQWLKDETALDEEKLRARILAEADRR
                     HAEKSVSIGPDVMRHLEKAIMLQVLDTQWKDHLAAMDYLRQGIHLRGYAQKNPKEEYK
                     RESFMLFSDMLNRVRHDAINLLAHVQVQAPQDVEALESQNRPPQEMQFVHPVLGAGGQ
                     PEEGESGEDVAVAQKPVTRRQPKIGRNDPCPCGSGKKYKHCHGKLS"
     gene            complement(4173..5099)
                     /locus_tag="AC16FV_10065"
     CDS             complement(4173..5099)
                     /locus_tag="AC16FV_10065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR2850436.1"
                     /translation="MNIILVPNSQRGKGRNTTLSHRHLVLIALILLLALPVLVGIVAY
                     HTQDMLSAYNADSQLAAQRRELAAQRTAVAEAKRNAEAHLNALAQRMGQMQAQLMRLN
                     ALGSRLTRMAGLDAREFNFSVEAAMGGPEKAAASSNPPELMDSLDRLTREIERQQERL
                     SALENLLLDRKLNAAVTPSGWPVEGGWISSGFGVRADPFNGHQSYHEGVDIASNMGSP
                     VLAMGDGVVTHSGEKAGYGLLVEVTHESGLVTRYAHSSAVLVRVGDRVQKGQSIALVG
                     TSGRSTGPHLHFEVVRNGASVNPMRYLQQAGK"
     gene            complement(5140..6051)
                     /gene="lpxC"
                     /locus_tag="AC16FV_10070"
     CDS             complement(5140..6051)
                     /gene="lpxC"
                     /locus_tag="AC16FV_10070"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR2850437.1"
                     /translation="MIKQRTLKNVIRATGVGLHTGEKVYLTLRPASSDTGIVFRRIDL
                     ADPVEIRACPENVSDTRLSTTLEYNGARVSTVEHLMSAFAGLGIDNAYVDLTAAEVPI
                     MDGSAGPFVFLVQSAGIAEQRAPKRFMRIKKEVRVEEGDKWACFQPFEGFKVSFAIEF
                     NHPTFRNSTQNATVDFSTTSFVKEVSRARTFGFMGDLEALRAVGLARGGGLDNAIVLD
                     EYRILNDDGLRYEDEFVKHKVLDAIGDLYLLGHPLIGAFSAHKSGHSLNNRLLRELVT
                     NQEAWELMTYNENDEAPIAFVRTAPAT"
     gene            complement(6398..7537)
                     /gene="ftsZ"
                     /locus_tag="AC16FV_10075"
     CDS             complement(6398..7537)
                     /gene="ftsZ"
                     /locus_tag="AC16FV_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR2850438.1"
                     /translation="MFELMDTYGQQAVIKVIGVGGGGGNVVDHMVSASIEGVEFINAN
                     TDSQALKRSQAKTILQLGTNLTKGLGCGANPDIGRQAAVEDRERIAEVLSGADMVFIT
                     AGMGGGTGTGAAPVVAQVAKDMNILTVAVVTKPFPFEGRKRMGIADQGIADLGQYVDS
                     IITIPNEKLLTVLGKDATLLDAFGKANQVLQNAVQGIAELITRPGLINVDFADVRTVM
                     SEMGMAMMGAASASGPSRAREAAIAAVSSPLLEDINISGARGILVNITAGLDMSIGEF
                     EEVGNAIKEFASDDATVVIGTVIEPEMREELRVTVVATGLGQEKARKQPYKVVKTGTT
                     GATDFEDYETPTVIRNRRTERPLATATEAAIEYLDIPAFLRKQAD"
     gene            complement(7601..8842)
                     /gene="ftsA"
                     /locus_tag="AC16FV_10080"
     CDS             complement(7601..8842)
                     /gene="ftsA"
                     /locus_tag="AC16FV_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR2850439.1"
                     /translation="MAKRDDEKLIVGLDIGTSKVLAIVGEIAPTGEVEIIGVGHHPSR
                     GMKKGVVVNIESTVQSIQRAVEEAELMAGCQIHSVYAGIAGSHISSFNSHGIVAIKDK
                     EVGQGDVERVIEAARALAIPADQKILHILPQEFIIDKQEGIREPVGMSGVRLEAKVHI
                     VTGAVSAAQNIVKCVRRCGLEVDDIILEQLASSISTLTEDEKELGVCLVDIGGGTTDI
                     SVFTDGAIRHTAVIPIAGDQVTNDIAVALRTPTQYAEDIKKKYGCALTQLAHRDETIE
                     VPGVGDRPPRKLSRQTLAEVIEPRIEELYSLVQAELRRSGFEDVIGSGLVLTGGSAKM
                     EGVVDLAEEVFHMPVRLGVPQYVGGLKGVVQNPIFATGVGLVLYGARCREGKQYVQNP
                     GVAVGVKGVWSRMKSWFQGNF"
     gene            complement(8845..9660)
                     /locus_tag="AC16FV_10085"
     CDS             complement(8845..9660)
                     /locus_tag="AC16FV_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR2850440.1"
                     /translation="MSSAETARTKSDEGISLFKAAVLLFALLSGLLVLAFGVDWLLRT
                     DSFPVRHVRFEGEFKHVTQQELEAAAMNVVRGNFFLLNLDTVKARVESLPWIYQASVR
                     REWPQDVHVRFTEQQLAARWGDGAFLNQAGDVVRVTLNDPLSELPRLEGPEGTGPQVL
                     EHYKKLGQILAVAGLKLERITLTPRRVWRLELADGMVVVLDRDQPEYKLERFARVYAQ
                     SLKHQAVNIAQVDLRYANGFAVQQGAARVAGHRDVSVRTDAAGAGRTGAAKKG"
     gene            complement(9647..10564)
                     /locus_tag="AC16FV_10090"
     CDS             complement(9647..10564)
                     /locus_tag="AC16FV_10090"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR2850441.1"
                     /translation="MSRNFGKVAVLMGGPSAEREVSLKSGKAVLAALKRKGVNAHGFD
                     ADKSTLRQLEDGKFERVFIVLHGRWGEDGVIQGALEVLDIPYTGSGVLGSALGMDKLR
                     TKYLWSAAGIPTPEYAVLDAGTDFDKVAAKLGLPVFVKPVREGSSLGVSKAKTVVELK
                     AAWKSAAKYDDQVIAERFIDGAEITCGILGDRALPLIKIETDREFYDYEAKYLLDTTR
                     YLCPCGLPADQERAIQALAQRAFSALGCSGWGRVDFMLDKSGRAYVLEVNTVPGMTDH
                     SLVPKAAQQAGMDFDELVLRILETADVER"
     gene            complement(10561..>11132)
                     /gene="murB"
                     /locus_tag="AC16FV_10095"
     CDS             complement(10561..>11132)
                     /gene="murB"
                     /locus_tag="AC16FV_10095"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR2850442.1"
                     /translation="EHELVGAEFLAGIPGTVGGALAMNAGAFGGETWNIVEAVETIDH
                     HGERRTRIPSDYKIAYRKVSGPQGEWFVAAHFRLASGDTSRGKTLIKTLLAKRGATQP
                     TQLPNAGSVFKNPPGDHAARLIETSGLKGACEGKACVSELHANFIVNQGGATAAEIER
                     LIARIQTTVENLHGVTLEPEVRVIGEAQT"
BASE COUNT         2028 a         3540 c         3294 g         2270 t
ORIGIN      
        1 ggtgagggta aggcggagat ttcacgaacg tccggtggac gttcgtgccg ccgaacgtgc
       61 ccgccaggga tggcggcccc gggctttccc gcggagccgg aggcgcagcg ggaaaggagc
      121 gcagcgcgca gcagtttttg agacagcttt aactgcggta ctcggcgttg atgcgcacat
      181 aatcgtgcga aagatccgaa gtccacacgc gcgcggaggc tgtacccgcc cccagttcga
      241 ccccgatgcg gatttccggc agacgcatca ccgcaagtcc cttggcctcg gtataaccgg
      301 cgcccggtgc accgttatgc accacacaca gctcgttaag gtggatcgtc accctggcaa
      361 catccagttg cgcgattggc gcgcgcccga ttgccgccag aatgcggccc cagttgggat
      421 cgctggcaaa aaaggctgtc ttcaccaggg gggaatgcgc aatggtgtag gccaccgcga
      481 ggcaatcgga ctcgctacgg ccacccctga cctcaatggt aatgaacttg gtagcgcctt
      541 cggcgtcgcg cacgatggct tgggcaagct ccatgaaaac ttccgtaaca gcaccgagta
      601 gcgccgcata aacatttcct tccgcccgtt caatcacggg atttccggcc gctccagtgg
      661 ccagcagaac acaagcatcg ttggtcgagg tatcgccgtc caccgtgatg cgattgaagg
      721 attgattcac tgcggtcgtg acacaagcat gaagcaccgc tggtgcaacg gcagcgtccg
      781 tcgccacgaa cgccagcatg gtcgccatgt caggtcggat catgccggcg cccttggcga
      841 tgcctgtcac cgtgatcgtg cgtgaatcga ttttgatctg gcgcgagctg cccttcggca
      901 aggtatccgt tgtcatgatg ccatgcgccg cttcggtcca gccgttggca tccagattgg
      961 caagacagga tggtaacccg gagagcaacc gttcacgcgg aagtcgctct ccgatcacgc
     1021 ccgtggaaaa tggtaggatt tccgtggcgc ggcaattcat cccgctcgcc agagcctcgc
     1081 aacaggcgcg cgcatcttcc atgcccggct cgccggtgcc ggcattggcg aatccggtat
     1141 tgatgagcag ggctcgcggt gctctcttcg cgagatgttc acgcgccacc gtgacgggcg
     1201 cggcacagaa acggttttgc gtgaaggtgg cggacgcttg cgtgccgggc gtgcactcga
     1261 tcaccaccag atcgcgccgg tcttttttgc ggatgccggc ggcggtagtt ccgaggcgga
     1321 tgccgggaac gggatgcagc accggcaatc catgcagacc gaccgccatg gatcagctca
     1381 gctttccgtg gcagtgcttg tatttcttgc ccgaaccgca gggacaggga tcgttacgcc
     1441 cgatcttggg ctggcggcgg gtaacgggtt tttgcgccac cgccacgtct tcgccactct
     1501 cgccctcttc cggctggccg ccagcaccaa gcaccggatg cacgaattgc atctcctgag
     1561 gcgggcgatt ctggctttcc agcgcttcga catcctgtgg cgcctgcacc tggacatgcg
     1621 ccagcagatt gatggcgtcg tgccgcacac ggttcagcat gtcggagaaa agcatgaagg
     1681 actcgcgctt gtactcttcc ttcggatttt tctgggcata accgcgcagg tgaatgccct
     1741 gacgcaggta atccatggcg gcaagatgat ccttccactg ggtatccagc acttgcagca
     1801 tgatcgcctt ttccaggtga cgcatgacgt cgggaccgat ggaaacgctc ttttcggcat
     1861 gccggcggtc ggcttcggcg aggatacgcg cccgtaattt ctcttcgtcg agagccgtct
     1921 catccttcaa ccattgcgct atgggaagat tcaaacccag ctcgcgttcc agactctctt
     1981 ccagtccgga gatatcccat tgttcctcca ggctctccgg cggaacatgc tggctgatca
     2041 actcgttcac gacatcgtga cgtatggtgg tgatgctttc cgagatgtct tccacgtcca
     2101 tgagccggtt gcgctgttcg tagatcacct tgcgctggtc gttggccacg tcatcgtatt
     2161 ccagaagctg tttgcggatg tcgaagttgc gcccctcgac cttgcgctgg gcgttctcga
     2221 tggcgcgcgt cacccatggg tgctcgatgg cctcgccctc ctgcattccg agtttctgca
     2281 tgagaccgga gaggcgatcg cttccgaaga tgcgcaacag attgtcttcg agtgaaagat
     2341 aaaaacgcga ggaaccggga tcgccttgac gaccggaacg gccgcgcaac tgattatcga
     2401 tacggcgtga ttcatgccgt tccgtgccga tcatatgcag gccgcccgcg gcgataacct
     2461 gctgatggcg cttctcccac tcggcgcggg catgctcacg cttggccttg tcgtcgccga
     2521 tttccttcag ctccatctcg agattgccgc cgagcacgat gtcggtgccg cgaccggcca
     2581 tgttggtggc gatggtcagg gcgccgggac ggccggcctg cgccacgatc atcgcctcgc
     2641 gttcatgatg cttggcgttc aatacctgat gctcgatctt ctctttctgc aacaggcgcg
     2701 agaggtgctc cgaagcatcg attgaaacgg taccaaccag caccggctgg ccgcgttgat
     2761 gacagtcacg gatatccacc aggatcgccg cgtgtttttc cgcggcggtg cggtagatct
     2821 ggtcggccat gtcctggcgg accatcggcc ggtgggtcgg gatcaccacc acctcaagcc
     2881 cgtaaatctg ctggaactcg aaagcctcgg tatcggccgt gccggtcatg ccggcgagct
     2941 tgccatacag tctgaaataa ttctggaacg tgatggttgc cagcgtctga ttctcgttct
     3001 ggattgcgac gccttccttc gcctccacgg cctggtgcag gccatccgac cagcgccgcc
     3061 cctgcatcat gcgcccggtg aattcgtcga tgatgatgac ttcgttgtcg cggacgatgt
     3121 aatccacctc gcgcttgaac agcgcgtgcg cgcgcagcgc ggcgatgaga tggtgcatca
     3181 atatgaggtt cgatacgtcg tacagactcg aaccggccgc cagcaaaccg gcctgggcca
     3241 gcagtttctc gaccctttca tggccggcct cggtcagata ggcctggcgg gacttctcgt
     3301 ccacgctgta atcgcccggc ccattctcgg tttcctgctt cgtcaggtgc ggaatgatcg
     3361 tattgacctt gacgtacaga tcggtgcttt cctcggccgg cccggagatg ataagcggtg
     3421 tgcgtgcctc gtcgatcaga atcgagtcaa cctcgtccac gatggcgtag ttgagtccgc
     3481 gctgtgcgcg ttcctccgcg cgaaacgcca tgttgtcgcg caggtaatcg aaaccgtatt
     3541 cattgttggt gccataggtg atgtcggcgg cgtaggcttc gcgcttggtc tggcggtcct
     3601 gccccggaac aaccacgccc acgctcatgc cgagaaactt gtaaatctgc cccatccact
     3661 cggcgtcgcg ccgggccagg taatcgttga cggtgaccac atgcacgccc ttgcccgaaa
     3721 gcgcgttcag gtagacaggc agcgtcgcga ccagcgtctt gccctcgccg gtccgcatct
     3781 ccgaaatctt gccctggtgc aacaccatgc cgccgatcag ctgcacgtcg aaatggcgca
     3841 tgcccagcgt gcgcttggag gcctcgcgca ccaccgcaaa ggcctcgtgc aaaataccat
     3901 cgagggtatc gccgttcgag agccgggttc tgaattcggc ggtcttggcg cgcaactgct
     3961 cgtcggtgag ttgcgcaatg ccggtttcga gtgcattgat gcgcgccaca tcctgctgca
     4021 tgcgcttgat gaggcgggtg ttgcgactgc cgaatatctt ggtaagcgct ttcgtaatca
     4081 taaaaatccg tgcgcctcgc gcggaggcgt atcaaaagag cggcaattat ggaggaaaag
     4141 tggagagact tgaagcgtga cggcgttaca acctactttc cggcttgctg caaataccgc
     4201 atggggttca ctgaagctcc attgcgcacc acctcgaaat gcagatgggg cccggtggag
     4261 cggccggaag tgccgaccag cgcgatggac tggcccttct gcacgcgatc gccgaccctg
     4321 acgaggacgg cgctggaatg ggcgtaacgg gtgaccaaac cggattcgtg ggtgacctcg
     4381 accagcaatc cataaccggc cttctcgccg ctgtgagtga cgacgccatc gcccatggcc
     4441 aatacgggcg agcccatgtt cgaggcaatg tccacgcctt catgataaga ctgatggcca
     4501 ttgaagggat cggcgcgcac gccgaagccg gaggatatcc acccgccctc caccggccag
     4561 ccggaaggcg tcacggcggc gttgagcttg cgatccagca gcaggttttc gagcgcgctc
     4621 aggcgctcct gctgacgctc aatctcgcgg gtgagacgat cgagcgagtc catcagttcg
     4681 ggcggattcg atgacgcggc cgccttttcc gggccgccca tggcggcctc gacggagaaa
     4741 ttgaattcac gcgcgtccag accggccatg cgcgtcagac gcgaacccag ggcattgagt
     4801 cgcatcagct gcgcctgcat ctgacccatg cgctgcgcca gcgcgttgag atgcgcctcg
     4861 gcattacgct tggcctccgc cacggccgtg cgctgcgccg ccagctcgcg acgctgcgcc
     4921 gctagctgcg aatccgcgtt atacgcggag agcatgtcct gggtgtgata ggcaacgata
     4981 ccgaccagca ccggcaacgc gagcaggagt atcaaggcaa tcaggacaag atgacgatgg
     5041 gaaagcgtgg tattgcggcc tttgccacgc tgactgttcg gtaccaggat gatattcatg
     5101 ccgcgcttcc cctgtcgtct cgccgttcgc tcgcgcgcct caagttgccg gagcggtgcg
     5161 aacgaaggcg atgggcgcct cgtcgttctc gttataagtc atgagttccc acgcttcctg
     5221 gttcgtcacc agctcgcgca gcagacggtt attgagcgaa tgaccggact tgtgggcgct
     5281 gaaggcaccg atcagcggat gtcccagcag atacagatcg ccgatggcat ccagcacctt
     5341 gtgtttgacg aattcgtctt catagcgcaa accgtcgtca ttcaggatac ggtattcatc
     5401 cagcacgatg gcgttgtcga gaccgccgcc gcgcgccagc ccaaccgcac gcagtgcctc
     5461 gaggtctccc atgaaaccga aggtgcgggc gcggctgacc tccttgacga aggaagtcgt
     5521 ggaaaaatcg acggtggcat tctgggtcga gttgcggaag gtcgggtgat tgaattcgat
     5581 ggcgaaggaa accttgaagc cctcgaacgg ctgaaagcag gcccatttgt cgccctcttc
     5641 cacgcgcacc tccttcttga tgcgcatgaa acgcttcggc gcgcgctgct cggcgatgcc
     5701 ggccgactgc accaggaaca cgaacggacc ggcgctgccg tccatgatcg gcacttccgc
     5761 cgccgtcaga tcgacgtagg cgttatcgat acccagaccg gcgaatgccg acataagatg
     5821 ctccaccgtg gaaactctcg caccgttata ttcaagtgta gtcgagaggc gcgtgtcgga
     5881 gacgttctcc gggcaggcgc gaatttccac cggatctgct aagtcgatgc ggcgaaagac
     5941 aattcctgtg tcggaagagg ctggacggag ggtcagatag actttttcgc ctgtgtggag
     6001 acccacgccg gtggcgcgga tgacgttttt cagagtccgt tgtttaatca tcaaaacatc
     6061 ttctcgaatg atccctttct tgagaaaagg cgcgcgttcc tccgccgggg cggaaaatat
     6121 tcacgctaag ttaatttggt gcacgcaacc ctgcatgccg attacgattt cactcgggta
     6181 aggtcaatgg aaaaatcagt ccctacccgt aatttaagga taaaacccca catctgtcca
     6241 gccaaatctc ggatcgccga ccgcaatcgc gaaactatgc ccgataaacg gcccgtttcg
     6301 cggtgaatgc ggcttcaccg gcgggccctg tggcgcaaga acaacctctt ccatggcgaa
     6361 gctcccgccg cggagcgttc gccgtgggta atgggcctta atccgcctgc ttgcgcaaga
     6421 acgccgggat atccagatac tcgatggccg cctcggttgc cgtggccaac ggccgttccg
     6481 tgcgccggtt gcgaatcacc gtcggggtct cgtagtcctc gaaatcggtt gcgccggtgg
     6541 taccggtctt gaccactttg tatggctgct tgcgcgcctt ttcctgaccc aggcccgtgg
     6601 ctacgacggt gacgcgaagc tcttcgcgca tttcgggctc gatcacggtg ccgataacca
     6661 cggttgcatc gtcggaggcg aactccttga tggcgttgcc gacctcctcg aactcgccga
     6721 tggacatgtc gagaccggcc gtgatgttga ccagaatgcc gcgggcgccg gagatattaa
     6781 tgtcttccag cagcgggctt gagacggccg cgatggccgc ttcgcgtgcg cgcgacgggc
     6841 cgctggcgga agccgcgccc atcatggcca tgcccatttc cgacatcacg gtacgcacgt
     6901 cggcaaagtc cacattgata agtcctggcc gcgtgatcag ttcggcaatg ccctgtaccg
     6961 cgttttgcag tacctggttg gccttgccga aggcatccag caaggttgcg tccttgccga
     7021 gtaccgtcag tagcttctcg ttcgggattg ttattatgga gtcgacgtat tgacccaggt
     7081 cagcgatacc ctggtctgca atccccatgc gcttgcgccc ctcgaacgga aacggcttgg
     7141 tcacgacggc caccgtcagg atgttcatgt ccttcgcgac ctgcgccacc accggtgcgg
     7201 cgccggtgcc agtgccgccg cccatgccgg cggtgatgaa caccatgtcc gcgcccgaaa
     7261 gcacttcggc gatgcgctcg cgatcctcga cggcagcctg gcggccgatg tcgggattgg
     7321 cgccgcagcc aaggcccttg gtgaggttgg tgcccaattg aagaatggtc ttcgcctgcg
     7381 agcgcttgag cgcctgcgaa tcggtgttgg cgttgatgaa ctccacgcct tcgatgctgg
     7441 ccgagaccat gtgatcgacg acattgccgc cgccgccgcc cacgccgatg accttgataa
     7501 ctgcttgttg cccgtaggta tccattagtt caaacatgtg ttgcctccag tgtaggtttc
     7561 cttgaatccg ttgatcgaac catctgcctg ttcccccgca tcaaaaattc ccctgaaacc
     7621 agctcttcat cctgctccag actcccttca ctcccacagc cacgcccggg ttctgcacgt
     7681 actgcttgcc ctcccggcaa cgcgcgccgt acaacaccag ccccacgccg gtggcgaaga
     7741 tcgggttctg caccacgccc ttcaaaccgc cgacgtactg cggcacgccc aggcgcaccg
     7801 gcatgtgaaa cacttcttct gcgagatcga ccacgccctc catcttggcg ctgccgccgg
     7861 tgagcacgag accgctgccg atcacgtctt cgaaaccgct gcgacgcagc tctgcctgca
     7921 ccaagctgta gagctcctcg atgcgcggct cgatgacctc ggccagcgtc tggcgcgaca
     7981 acttgcgcgg tgggcggtca cccacgcccg gtacctcgat ggtttcgtcg cgatgcgcca
     8041 gttgcgtcag ggcgcagccg tattttttct tgatgtcctc ggcgtattgc gtcggcgtgc
     8101 gtagcgccac ggcgatgtcg ttcgtcacct gatcaccggc gatgggaatc acggcggtgt
     8161 gacggatggc accgtcggtg aacaccgaga tgtcggtggt gccgccgccg atgtccacga
     8221 ggcacacgcc cagttccttt tcgtcctccg tcaacgtgga gatgctggaa gcgagctgtt
     8281 cgaggatgat gtcgtccact tccagcccgc agcggcgtac gcacttgacg atgttctgcg
     8341 ccgcgctgac cgcgccggtg acgatgtgca ccttggcctc gagccgcacg ccgctcatgc
     8401 cgaccggttc gcgaatgcct tcctgcttgt cgatgatgaa ttcctgcggc aggatgtgca
     8461 ggatcttctg atcggccggg atggcaagcg cgcgcgccgc ttcaatcacg cgctctacgt
     8521 caccctggcc gacttccttg tccttgatgg cgacaatccc atgcgagttg aagctggaga
     8581 tatgcgagcc ggcgatgccc gcgtaaaccg agtgaatctg gcagccggcc atgagctcgg
     8641 cctcctcgac cgcgcgctgg atcgattgca ccgtggactc gatgttcacc accacgccct
     8701 ttttcatgcc gcgcgagggg tgatgtccca cgccgatgat ctcgacctcg cccgtcggcg
     8761 cgatttcacc aacgatggcc agcaccttgg aggtgccgat gtcgaggcca acgatcagtt
     8821 tttcatcgtc tcttttcgcc ataattaccc cttcttggct gctcctgtcc gtcctgcacc
     8881 ggcggcgtcc gtgcgtaccg acacgtcccg atgaccggcg acgcgcgcgg cgccctgctg
     8941 taccgcaaac ccattggcat agcgcagatc cacctgtgcg atgttcacgg cctgatgctt
     9001 cagcgattgc gcatacacgc gcgcgaaacg ttccagcttg tactccggct gatcccgatc
     9061 cagcacgacg accattccat ccgccagctc cagtcgccag acgcggcgcg gcgtcaatgt
     9121 gatgcgctcc agcttcagcc cggccacggc cagaatttgt ccgagcttct tgtagtgttc
     9181 gagcacctgc ggcccggttc cttccgggcc ctccaggcgc gggagttcag acaacggatc
     9241 gttcaacgtc acgcgcacta cgtcaccggc ctgattcagg aaggcgccgt cgccccagcg
     9301 cgcggcgagc tgctgctcgg tgaagcggac atgcacgtcc tgcggccact cgcggcgcac
     9361 cgaggcctgg taaatccagg gcagcgattc cacgcgcgcc ttcaccgtgt cgaggttgag
     9421 caggaagaaa ttgccgcgca cgacgttcat ggcggcagcc tccagctcct gctgcgtcac
     9481 gtgcttgaac tcgccttcga aacgcacatg ccgcaccgga aaactatcgg tgcgcaacag
     9541 ccagtcgacg ccgaaggcca gcaccaacag cccggacagc agcgcgaaca gcagcaccgc
     9601 ggccttgaac aggctgatac cttcatcgct ctttgtgcgt gctgtctcag cgctcgacat
     9661 ccgccgtctc cagtattctc aaaaccaatt catcgaaatc catgcccgcc tgctgcgccg
     9721 ccttgggcac gaggctgtga tccgtcatgc ctggcaccgt gttcacctcg agcacgtagg
     9781 cgcgcccgct tttgtcgagc atgaaatcca cccgccccca gccgctgcaa ccgagcgcgc
     9841 tgaacgcgcg ttgcgccagt gcctggatcg cgcgctcctg atccgcgggc aggccgcagg
     9901 gacagagata gcgcgtggta tcgagcagat acttcgcttc gtagtcgtag aattcgcgat
     9961 ctgtttcaat cttgatcagc ggcagcgccc gatcgccgag aatcccgcag gtgatctcgg
    10021 cgccgtcgat gaaacgctcg gcaatgacct gatcgtcgta cttggccgcg ctcttccacg
    10081 ctgccttgag ttccaccacc gtcttggcct tgctgacgcc gagactggaa ccttcgcgca
    10141 ccggtttcac gaaaaccggc aggccaagct tcgcggcgac cttgtcgaaa tcggtgccgg
    10201 cgtcgagcac ggcgtattct ggcgtcggaa tgccggcggc ggaccagaga tatttggttc
    10261 tgagcttgtc catgccgagc gccgaaccca ggacgccgct gccggtatag ggaatgtcca
    10321 gcacttccag cgcgccctga atcaccccgt cctcgcccca gcgaccgtgc agcacaatga
    10381 acacgcgctc gaatttcccg tcttccagct gtcgcaaggt ggacttgtcg gcgtcgaaac
    10441 catgcgcatt gacccctttg cgcttcaacg cggcaagcac ggccttgccg ctcttgagcg
    10501 acacctcgcg ctcggcggaa ggaccgccca tcagcaccgc caccttgccg aaatttctgc
    10561 tcatgtttgc gcctcgccga taacacgcac ttcgggttca agtgtcacgc catgcaggtt
    10621 ttccaccgtg gtctgaatcc gtgcgatcaa gcgctcgatc tcggcggcgg tggcgccgcc
    10681 ctgattgacg atgaaattcg cgtgcaattc ggaaacgcag gccttgccct cgcacgcgcc
    10741 cttcagtccg ctggtctcga tcaggcgcgc ggcgtgatct cccgggggat tcttgaacac
    10801 cgacccggcg ttcggcagtt gcgtgggttg agtcgcgcca cgcttggcca gcaaggtttt
    10861 aataagtgtc ttgccccggg aggtatcacc actcgcaagg cggaaatgcg cggcgacaaa
    10921 ccattcaccc tgggggccgc tgactttgcg ataagcgatt ttgtaatccg agggtatgcg
    10981 cgtgcgccgt tcaccgtggt ggtcaatggt ttccaccgcc tcgacgatgt tccaggtctc
    11041 gccaccgaag gcgccggcat tcatggccag tgcgccgccg accgtgccgg gaatgccagc
    11101 caggaattcc gcgcccacca gttcatgctc gg
//
LOCUS       DBNGZE010000396         3026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1166597, whole genome shotgun sequence.
ACCESSION   DBNGZE010000396 DBNGZE010000000
VERSION     DBNGZE010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1166597"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1350)
                     /gene="secY"
                     /locus_tag="AC16FV_10100"
     CDS             complement(4..1350)
                     /gene="secY"
                     /locus_tag="AC16FV_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR2850443.1"
                     /translation="MSSPAGSFGGLAGLGKLTELRQRIFFVLGALIVYRIGTYIPVPG
                     INPVALAKMFEGTQSSIVGLFNMFSGGALQRFSLFALGIMPYISSSIIMQLMTSVIPT
                     LEQLKKEGESGRRKITQYTRYGTVVLATFQALGIAIALESQTSAGMPVVIAPGIGFKL
                     ITVISLVTGTMFLMWLGEQVTERGIGNGISILIFAGIVAGLPGAIAHTLELASRGTFN
                     PLFVLTLFAIALAVTAFVIFVERGQRRITVNYAKRQQGRRVMAGQTSHLPLKINMSGV
                     IPPIFASSIILLPATVVSWFGQAEGMDWLRNIATTLSPGQPIYVILYASAIVFFCFFY
                     TALVFNPKETAENLKKSGAFIPGIRPGDQTAGYIDGVMSRLTLGGAIYVTLVCLLPEF
                     MIVKWNVPFYFGGTSLLIVVVVCMDFMAQVQARLMSHQYESLLKKANLTGGMGLPR"
     gene            complement(1392..1832)
                     /gene="rplO"
                     /locus_tag="AC16FV_10105"
     CDS             complement(1392..1832)
                     /gene="rplO"
                     /locus_tag="AC16FV_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR2850444.1"
                     /translation="MRLNSMKPGRGAKTSPKRLGRGGGSGLGKTSGKGHKGQTARAGG
                     YHKVGFEGGQMPIQRRLPKRGFRSAMKVSGQTAEVRLQELGKLEEAVIDLAALKKANV
                     VPAQAEQAKVYLSGTLEKAVTLRGIRLTKGAREAVMKVGGKVED"
     gene            complement(1835..2035)
                     /gene="rpmD"
                     /locus_tag="AC16FV_10110"
     CDS             complement(1835..2035)
                     /gene="rpmD"
                     /locus_tag="AC16FV_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR2850445.1"
                     /translation="MAANPKSGKKLRVTLLKSPFGTGKSHMATVRGLGLRRRHHTVEL
                     EDTSAVRGMINKVAYMVKCEEV"
     gene            complement(2046..2555)
                     /gene="rpsE"
                     /locus_tag="AC16FV_10115"
     CDS             complement(2046..2555)
                     /gene="rpsE"
                     /locus_tag="AC16FV_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR2850446.1"
                     /translation="MAAFSESEARSDNFQEKLISVRRVAKVVKGGRIFGFSALTVVGD
                     GAGRIGIGRGKAREVPAAVSKAMENARRNMMKVQLKGPTLHHAVTAAHGASKVVIKPA
                     SVGTGIIAGGAMRAVFEVVGVQDVLAKCLGSRNPVNMVRATVKGLQAMHSPESVARKR
                     GKRVEEILG"
     gene            complement(2566..2922)
                     /gene="rplR"
                     /locus_tag="AC16FV_10120"
     CDS             complement(2566..2922)
                     /gene="rplR"
                     /locus_tag="AC16FV_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019894805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR2850447.1"
                     /translation="MKREIDTRLRRARHIRRKIAGLQADRLCVHRTPRHIYAQIIDKT
                     GSKVLASASTVEADVRGQVKHGGNVEAAKLIGKRIAEKAKTAGITRVAFDRSGFKYHG
                     RVKALADAARENGLEF"
     gene            complement(2951..>3026)
                     /locus_tag="AC16FV_10125"
     CDS             complement(2951..>3026)
                     /locus_tag="AC16FV_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016955060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR2850448.1"
                     /translation="PEPYKGKGVRYVNEVVVKKEAKKK"
BASE COUNT          584 a         1010 c          836 g          596 t
ORIGIN      
        1 ctgttagcgc ggcagaccca taccgccggt caggttcgcc tttttcagca ggctctcata
       61 ctggtgcgac atgagccggg cctgcacttg ggccatgaaa tccatgcaca ccaccaccac
      121 gatcagcagg gaagtgccgc cgaaataaaa cgggacattc cacttcacga tcataaactc
      181 gggcaacagg cacaccagcg tcacgtaaat cgccccgccc agtgtcagtc gcgacatcac
      241 cccgtcgatg taacccgccg tctgatcgcc gggacgtatg cccgggatga acgcgccgga
      301 cttcttcagg ttctccgccg tctccttggg attgaacacc agggcggtat agaagaaaca
      361 gaagaacacg atcgccgacg cgtacagaat cacgtaaatc ggctgaccgg gcgacaaggt
      421 cgtcgcgatg ttacgcaacc aatccatgcc ctccgcctga ccgaaccagc tcacgaccgt
      481 ggccgggagc aggataatgc tcgaggcgaa gatcggcggg atcacgcccg acatgttgat
      541 cttgagcggc agatggctgg tctgtccggc catcacccgg cgtccctgct ggcgtttggc
      601 gtaattcacc gtgatacgcc gctggccgcg ctcgacgaaa atcacaaacg ccgtcaccgc
      661 cagtgcaatc gcaaacaacg tcagtacgaa caaggggttg aacgtgccac ggctggccag
      721 ctccagcgta tgcgcgatgg cccccggcaa tcctgccacg ataccggcaa aaatcaggat
      781 cgagatgccg ttgccgatgc cacgctcggt gacctgttcg ccgagccaca tcaaaaacat
      841 cgtgccggtc acgagcgaaa tcactgtgat cagcttgaaa ccaattcccg gcgcaatcac
      901 caccggcatg ccggccgaag tctggctctc cagcgcgatg gcgatgccca gcgcctgaaa
      961 ggtggccagc accaccgtgc cgtagcgggt gtactgcgtg atcttgcgcc gcccgctctc
     1021 gccctctttc ttcagctgtt ccagcgtcgg gatcaccgac gtcatcagct gcatgatgat
     1081 tgacgacgag atgtacggca tgatccccag cgcaaacagc gaaaaccgct gcagcgctcc
     1141 gccggaaaac atgttgaaca gtccgacgat ggagctttgt gtgccctcga acatcttggc
     1201 cagggccacg ggattgatgc ccggcaccgg tatataggta ccgatgcgat aaacgatcaa
     1261 tgcccccaga acaaagaaaa tccgctgccg cagctccgtg agcttgccca agcccgcgag
     1321 cccgccgaag cttcctgctg ggctactcat atgaaaagac gaacgagcga agcgagtagg
     1381 gcttcattgc cttagtcttc gaccttcccg ccaaccttca tcaccgcctc acgcgcaccc
     1441 ttggtcagac ggatgccacg cagcgtgact gctttctcca gcgtaccgga gagataaacc
     1501 ttggcctgct ccgcctgcgc cggaacgaca ttcgccttct tgagcgcggc cagatcgatc
     1561 acggcctctt ccagcttgcc cagttcctgt aaccgaacct cggcggtctg gccgctcacc
     1621 ttcatggcgg aacgaaaacc gcgtttcggc aggcgccgct ggatcggcat ctgaccgcct
     1681 tcgaagccca ccttgtgata accgccggca cgcgcggtct ggcccttgtg gcccttgccg
     1741 ctggtcttgc cgaggccgga gccgccgccg cgtccgagac gcttgggcga ggttttggca
     1801 ccacgacccg gtttcattga attcagacgc atggtcatac ttcctcgcac ttcaccatat
     1861 aggcgacctt gttgatcatg ccacgcacgg cggaagtgtc ttccagctcc accgtgtgat
     1921 gacgccggcg caggcccaaa ccgcgcaccg tggccatgtg gctcttgccg gtgccgaacg
     1981 ggctcttgag cagcgtcacg cgcagtttct ttcctgattt cggatttgcc gccatggttt
     2041 gccccttagc ccagaatctc ttccacgcgc ttgccgcgct tgcgtgcgac cgactccgga
     2101 ctgtgcatcg cctgcaggcc cttcacggtg gcgcgcacca tgttcaccgg attgcgcgaa
     2161 cccaggcact tggccagcac gtcctgcacg cccacaacct cgaacacggc gcgcatggcg
     2221 ccgcccgcaa tgatgccggt accgaccgag gctggcttga tcacgacctt ggaagccccg
     2281 tgagcggcgg taaccgcgtg atgcagcgtc ggccccttga gctggacctt catcatgtta
     2341 cggcgcgcgt tttccatggc cttgctgacc gccgccggca cttcgcgcgc cttgccgcga
     2401 ccgatgccga tgcgaccggc gccatcaccc accacggtca acgcggagaa gccgaaaata
     2461 cggccgccct tgacgacctt ggccacgcgc cggacgctga tgagtttttc ctggaaattg
     2521 tcgctccgtg cttcggattc gctgaaagct gccacgatat cgtcctcaga attccaaacc
     2581 gttttcgcgc gccgcgtcgg ccagtgcctt cacgcgccca tggtacttga agccggagcg
     2641 gtcgaaggcc acccgggtga tgcccgcggt tttcgctttt tcggcgatgc gcttgccgat
     2701 cagtttcgcc gcctcgacgt tgccgccgtg cttgacctgg ccgcgcacat cggcctcgac
     2761 cgtggaggcc gacgccagta ctttgctgcc agtcttgtcg atgatctggg catagatatg
     2821 acgcggcgtg cgatgcacgc acagacgatc ggcctgaagg ccggcgattt tccgccggat
     2881 atggcgcgcg cggcgcaacc gtgtatcgat ttctcttttc ataaaattcg tttacctgac
     2941 tcgaagcccg ttacttcttc ttggcttctt tcttaactac gacttcattg acgtagcgca
     3001 cgcccttacc cttatacggt tccggt
//
LOCUS       DBNGZE010000397         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1930017, whole genome shotgun sequence.
ACCESSION   DBNGZE010000397 DBNGZE010000000
VERSION     DBNGZE010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1930017"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="AC16FV_10130"
     CDS             <1..165
                     /locus_tag="AC16FV_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR2850449.1"
                     /translation="LAGNILIRQRGTEYHPGVNVGIGKDDTLFAKVDGQVKFEIKGPR
                     SRRTVSIIPG"
     gene            238..1278
                     /gene="cgtA"
                     /locus_tag="AC16FV_10135"
     CDS             238..1278
                     /gene="cgtA"
                     /locus_tag="AC16FV_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR2850450.1"
                     /translation="MKFVDEATIRVEAGAGGNGALSFRREKFIPRGGPDGGDGGNGGS
                     VHLIGQEGLNTLADFRHTRVFRAEKGAKGGGSNCTGKSGKDLYIQVPLGTRVMDAEAE
                     ELIGEITRNGESLLVAHGGKGGLGNTRFKSSTNRTPRRTTPGKPGERRELAFELQVLA
                     DVGLLGMPNAGKSTFLRAVSDARPKVADYPFTTLHPHLGVVRVGEHRSFVVADIPGLI
                     EGAAEGAGLGHQFLRHLSRTRILLHLVDMAPLEPDADPVAGVKALEAELKKFSQALAK
                     RERWLVLNKMDLLPEDERETRVKQIIKRLKWKRPVYRISAISGEGCRELTMDLMRQLE
                     QLNKKTEKHDEQ"
     gene            1281..>2099
                     /gene="proB"
                     /locus_tag="AC16FV_10140"
     CDS             1281..>2099
                     /gene="proB"
                     /locus_tag="AC16FV_10140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR2850451.1"
                     /translation="MSADLRDSLKSKRRIVVKIGSALLTRDGQGLNSDGIHDWAAQLA
                     QLRSRGLEVVLVSSGAVAAGMQRLGRSMRPSALHELQAMAAVGQMNLVQIYESAFQRH
                     GLHTAQVLLTHDDLTNRQRYLNSRTTLRTLLGFGVIPIINENDTVATEEIRFGDNDTL
                     AAPVANLIEADLLLILTDQRGLYDRDPRQDPDAKLLAEGRAGDPQLLSMAGGSGTLGR
                     GGMRTKLLAAEKAARSGASTIIASGREKNVIARVLDGEAIGTHLHAAQGRVAARI"
BASE COUNT          431 a          608 c          685 g          375 t
ORIGIN      
        1 ctggcgggca acattctcat ccgtcagcga ggcactgagt atcatcccgg tgtgaacgtc
       61 ggcattggca aggacgatac cttgttcgcc aaagtcgatg gccaggtgaa attcgagatc
      121 aagggaccgc ggagccgcag aacagtaagc atcatcccgg gctgacccgc ttacgcacgc
      181 attgaaaacc ccgccctggc aacacggcgg ggttttttat tttggaacat cgctcatatg
      241 aaattcgttg acgaagctac tattcgcgtc gaagccggtg ccggcggcaa cggtgcgctg
      301 tcgtttcggc gcgagaagtt tatcccgcgc ggcgggccgg acggcggcga cggtggcaat
      361 ggcggcagcg tgcatctgat cggacaggaa ggtctcaata cactcgccga tttccggcat
      421 acccgtgtgt ttcgcgccga gaagggcgcc aaaggtggcg ggtccaattg cacgggaaaa
      481 tcgggaaagg atctttatat acaggttccc ctgggtacgc gggtgatgga tgccgaggcc
      541 gaggaactga tcggagagat tacgcgcaac ggcgagtcgc tgctggtggc ccacggcggc
      601 aagggcggtc tcggcaacac gcgcttcaag tcgagcacga atcgcacccc gcgacgcacg
      661 acacccggca agccgggcga acgtcgtgag ctggcgtttg agcttcaagt gctggccgac
      721 gtcggtttgc tcggcatgcc caatgccggc aaatcaacat tccttcgagc ggtatcggat
      781 gcgcgtccca aggtggcgga ctatcctttt accaccctgc atccgcatct gggcgtggtg
      841 cgcgtcggcg aacatcgcag tttcgtggtg gccgacatcc ccggactcat cgagggtgcc
      901 gccgaaggtg ccggtctggg gcaccagttc ctgcggcatc tgtcgcgcac gcgcatcctg
      961 ttgcatctgg tggatatggc gccgctggaa ccggatgccg acccggtagc gggggtcaaa
     1021 gcccttgagg ccgagctcaa gaaattcagc caagcactcg ccaagcgcga acgctggctg
     1081 gtactaaaca aaatggatct gcttcccgag gacgaacgcg agacgcgcgt gaaacagatc
     1141 atcaagaggc tgaaatggaa acgcccggtg tatagaatct ccgccatcag cggcgagggt
     1201 tgccgcgagc tgaccatgga tctgatgcgg cagctggagc agctcaataa gaaaacggaa
     1261 aaacacgatg agcaatgaac atgagcgctg acctgcgcga ctcgctcaaa agcaagcgcc
     1321 ggatcgtggt aaaaatcggc agtgcgctgc tgacgcgcga cggtcagggt ctgaacagcg
     1381 acggtattca tgactgggcg gcgcagctcg cacagctgcg cagccgcggc ctcgaagtgg
     1441 tgctcgttag ttccggagcg gtggcggccg gcatgcagcg cctcggccgc agtatgcgtc
     1501 ccagcgcatt gcatgaatta caggccatgg cggcggtcgg gcagatgaat ctggttcaga
     1561 tttatgaatc cgcgttccag cgccacggtc tgcatacggc gcaggtcttg ctgacccatg
     1621 atgatctgac caatcgccag cgttatctca acagtcgcac cacgttgcgc accttgctcg
     1681 gcttcggcgt gattcccatc atcaatgaaa atgacacggt tgccacggag gaaatccgtt
     1741 ttggcgacaa cgacaccctg gcggcgcccg tggccaacct catcgaggcg gatttgctgc
     1801 tgattctcac tgatcagcgc ggtttgtatg accgggaccc gcgccaggat ccggacgcca
     1861 agctgctggc tgaaggcagg gccggcgatc cgcagttgct ttccatggcc ggcggctccg
     1921 gcacgcttgg acgcggcggc atgcgcacga agctgctggc ggcggaaaag gcggcgcgtt
     1981 ccggcgcctc gacgataatc gcctcggggc gggagaaaaa cgtgattgcg cgcgtgctgg
     2041 acggcgaggc gatcggcacg catctgcacg cggcgcaggg acgcgtggcg gcgcgcata
//
LOCUS       DBNGZE010000398         7316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1305117, whole genome shotgun sequence.
ACCESSION   DBNGZE010000398 DBNGZE010000000
VERSION     DBNGZE010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1305117"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16FV_10145"
     CDS             complement(<1..310)
                     /locus_tag="AC16FV_10145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR2850452.1"
                     /translation="MTAPLLVELLTEELPPKALARLIETFSRNLFEGLKEKGFLAVTV
                     EPKAYATPRRLAVVVSQVLEKQPDRKIERKGPAVATALDAAGRPTAALIGFAKSCGVE
                     L"
     gene            complement(307..1191)
                     /gene="glyQ"
                     /locus_tag="AC16FV_10150"
     CDS             complement(307..1191)
                     /gene="glyQ"
                     /locus_tag="AC16FV_10150"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR2850453.1"
                     /translation="MTFQELIFTLSRYWARQGCVILQPYDMEMGAGTFHTATFLRALG
                     PEPWKAAYVQPSRRPKDGRYGENPNRLQHYDQYQVVLKPSPDDIQDLYLDSLRAIGIN
                     TSQHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEVGSLPCKPVLGEITYG
                     LERLAMYLQGVENVFDLVWTKGTSYGDVYHQNEVEQSKYNFEHSNVNWLFQQFNDYES
                     EAKRLMEAGLPLPGFEMVMKCSHAFNLLDARGAISTTERAAYIGRVRNLARLVAQSYY
                     HSRERLGFPLLKQKKAKA"
     gene            complement(1336..2283)
                     /gene="mtgA"
                     /locus_tag="AC16FV_10155"
     CDS             complement(1336..2283)
                     /gene="mtgA"
                     /locus_tag="AC16FV_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR2850454.1"
                     /translation="MFWKRRRGKRGIKAFMRRSARTLRWTFRIFLLLLILDLVYLGLT
                     WPDWNKIANGPIPKSAFMREYEKQLAENKSLPRLRWQPVAFSNFPKHLQRAVILAEDS
                     RFYEHSGFDLIAFKEAMDYNLEKGRFVFGGSTISQQTVKNLFLSSSRNPLRKWHELIL
                     TWGMEHQLSKRRILDIYLNVAQFGRGIYGVQAASQAYWGISADQLTPAQAAELVATLP
                     SPEKNNPATHTRNFERRSKKLLALLERYPGDAAETVRERPFDLFLPPPENGSETPADN
                     GHEPLPDEPPVESGAEPPPADNSNEYPPVEERSIRPHSI"
     gene            2646..3737
                     /locus_tag="AC16FV_10160"
     CDS             2646..3737
                     /locus_tag="AC16FV_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HIR2850455.1"
                     /translation="MSLPASPIVSPRAAAAPGTPRKAVALISGGLDSMLAAKVMLEQG
                     IHVEGINFYTGFCVEGHTHAIRNHEHAKPKRNNALWTAEQLGIKLHIIEVIEEYKDVV
                     INPKHGYGANLNPCLDCKGFMVRKAREWMEKNGFDFIITGEVLGQRPMSQRRDTMPVV
                     ARESGAFDRLLRPLCAKLLPETLPEREGWVDRSKLLDFNGRSRKPQMALAAQYGFTDY
                     AQPAGGCCFLTNEQYSHKLADLWTTRGSKQYELDDIMLLKVGRHLRPRPNFKLIVGRD
                     EGENNFLNGYRKHFTHLYATSHQGPLVLIDGKVDDTDIELAARLAARFGKGREAETVT
                     LTVTDTAGNSRELQVTPMAANDIPPEWYL"
     gene            3865..4095
                     /locus_tag="AC16FV_10165"
     CDS             3865..4095
                     /locus_tag="AC16FV_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR2850456.1"
                     /translation="MSHFVLDARYLLCPMPVIRTQNRIAELQPGDTLEVQATDPGALH
                     DIPAWCRVHGHSVLATQHEANEVRIMLRVNKT"
     gene            4092..5261
                     /locus_tag="AC16FV_10170"
     CDS             4092..5261
                     /locus_tag="AC16FV_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR2850457.1"
                     /translation="MSSAGEHSHFDISSPERYTAARRVTWVSVFVNLLLTVAQVIIGY
                     LGNSQALVADGIHTLSDLLTDFMVLFALFHSKKAADAEHPYGHERIETATTLMLGVIL
                     FAVGAGIAIRAVMRLLETTALPTPDLITLWVALFTLVAKEGLFRYLMHTANRLDSNML
                     RANAWHSRSDAVSSLVVVVGIGGSRFGFTYLDAVAAIVVAVMIVKVAVELGWQSLGEL
                     VDTGLAADDLDAIRKTILSVSGVKALHLLRTRRVGGQALADVHIIVDDHVSVSEGHQI
                     SEAVRTKLIKEIAPMTDVMVHIDTEEDVEGPSCQGLPLRDEVLKRLDGYFRAIPEARR
                     IEHVTLHYLNGHIDIGLLLPLSVVADVSAAQALARRFAEAVQGDKQIGTIDVQFH"
     gene            5464..6876
                     /gene="glnA"
                     /locus_tag="AC16FV_10175"
     CDS             5464..6876
                     /gene="glnA"
                     /locus_tag="AC16FV_10175"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HIR2850458.1"
                     /translation="MSSGADVLKMIKDKKVKFVDFRFTDTKGKEQHVSVPAHTIETET
                     FEHGKMFDGSSISGWKGIQESDMILMPEAATAVMDPFMEEPTLILRCDVIEPSTMQGY
                     ERDPRSLAKRAEAYLKSTGVADTAFFGPEAEFFIFDDVRWGADISGSFVKIDSEEASW
                     NTEKVYEGGNLGHRPTVKGGYFPVPPVDSQQDIRSAMCLAMEEMGLSIETHHHEVATA
                     GQNEIGMLFNTLVKKADELQIYKYCVHNVAHSYGKTATFMPKPIVGDNGSGMHVHQSL
                     AKDGKNLFSGNEYGGLSQTALYYIGGIIKHARALNAITNSLTNSYKRLVPGFEAPVML
                     AYSARNRSASIRIPYVPNPKGRRIEVRFPDAGSNPYLAFAAMMMAGLDGIQNKIHPGE
                     AMDKDLYDLEPEEEKKIPTVCHALDQALEALDKDRAFLKAGGVFTDDVINAYIDLKMA
                     DVTRMRMTTHPIEFDMYYSV"
     gene            7086..>7316
                     /locus_tag="AC16FV_10180"
     CDS             7086..>7316
                     /locus_tag="AC16FV_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR2850459.1"
                     /translation="MLTGSSPQILDNLTTAVLTFDPRLRLTSINPAGEMLFEISAKKV
                     VGQSLTDLLPHARLAAKTLTETLESSHPFTARG"
BASE COUNT         1503 a         2241 c         2127 g         1445 t
ORIGIN      
        1 ccagctccac gccgcaggat ttggcgaagc cgatcaaggc cgccgtcggt cggcccgcgg
       61 catcgagcgc ggtcgccacc gccggtcctt tgcgttcgat cttgcggtcc ggctgttttt
      121 ccagcacttg ggaaaccacg accgcgagac ggcgcggcgt ggcataggcc ttcggctcga
      181 ccgtgacggc gaggaaacct ttttccttga ggccttcaaa aagattacgg ctgaaggtct
      241 ctatcagacg cgccagcgcc ttgggcggca gttcttccgt cagcagttcc accaacagcg
      301 gcgccgtcat gctttcgcct tcttctgttt gagcagcggg aaacccagac gctcgcgcga
      361 gtggtaatag ctctgcgcca ccagccgcgc caggttgcgc acgcgcccga tgtaggcggc
      421 gcgctcggtg gtggagatag cgccgcgcgc gtccagcaga ttgaaggcgt gcgagcactt
      481 catcaccatc tcgaagcccg gcagcggcag gccggcttcc atcaggcgct tggcctcgga
      541 ctcgtaatcg ttgaactgct ggaacagcca gttcacgttg gaatgctcga agttgtattt
      601 cgactgctcg acctcgttct ggtgatacac atcgccgtag ctcgtgcctt tcgtccacac
      661 cagatcgaac acgttctcca ccccttgcag gtacatggcg aggcgctcga ggccgtaggt
      721 gatctcgccc agcaccggct tgcaggggag cgagccgact tcctggaaat aggtgaactg
      781 cgtgacctcc atgccgtcga gccacacttc ccagccgaga ccccaggcgc cgagcgtggg
      841 cgattcccag tcgtcctcga cgaaccgaat gtcatgctga gatgtgttaa taccaatcgc
      901 gcgcagcgaa tcgagataaa gatcctggat gtcgtccggc gagggcttga gcaccacctg
      961 atactggtcg taatgctgca ggcggttcgg attttcgccg taacggccgt ctttcggccg
     1021 gcgcgacggt tgcacatagg cggccttcca cggctcgggc ccgagcgcgc gcagaaaggt
     1081 cgcggtgtgg aacgtgcccg cgcccatttc catgtcgtat ggttgcagga tgacgcaacc
     1141 ctgacgggcc cagtagcggg acagcgtgaa gatcagttcc tggaaggtca caaatgtcgt
     1201 cctacttcgt tcgtctggcc ataatggcag tcgccctact tcgttcgtct ggccataata
     1261 ggtgttttta tgcgggacgt ccggcttgtt tggccggcga ttataccctg ccccgtgacg
     1321 gggtacaccg ccgcttcaga tggaatgcgg ccggatcgag cgctcttcca ccggcggata
     1381 ctcattgctg ttgtccgccg gtggcggctc agcgccgctt tcgaccggtg gttcatccgg
     1441 caatggttca tgcccgttgt ccgccggtgt ctcgctgcca ttctccggcg gcggcaggaa
     1501 cagatcgaag gggcgctcgc gcaccgtctc ggcagcgtca cccggataac gttcgagcag
     1561 ggccagaagt tttttcgagc gccgttcgaa attacgcgtg tgggtggcag ggttgttctt
     1621 ttccggactc gggagcgtgg ccaccagttc cgccgcctgc gccggtgtca gctgatcggc
     1681 gctgataccc caataggcct gcgacgccgc ctgcacgccg tatatgccgc gcccgaactg
     1741 ggccacgttc agatagatgt cgaggatgcg ccgcttgctg agctgatgct ccatgcccca
     1801 ggtcaagatc agctcgtgcc acttgcgcag cgggttgcgc gaagacgaca gaaacaggtt
     1861 ttttaccgtc tgctgcgaaa tcgtgctgcc gccgaaaacg aagcggccct tttccaggtt
     1921 gtaatccatc gcctccttga aggcgatgag atcgaagccg ctgtgctcgt agaaccggct
     1981 gtcctcggcc aggatcaccg cgcgttgcag gtgcttgggg aaattcgaga acgccaccgg
     2041 ctgccagcgc aaccttggca agctcttgtt ctcggccagc tgtttttcgt actcgcgcat
     2101 gaaggctgac ttgggtatcg gcccgttcgc gatcttgttc cagtcgggcc aggtgagccc
     2161 gagataaacc agatcgagaa tcagcaacaa cagaaagatg cgaaaggtcc agcgcaaggt
     2221 gcgcgcggaa cgccgcataa aagccttgat gccgcgtttg ccgcgacgcc gtttccagaa
     2281 catgccgtag attcattcgt aaccgcaatg ccgctatttt agcacttcgc ccggcccggc
     2341 ccggaccatg aaatgcgccg cccgtctgct cccatcgggc cggggtttca atcaacacga
     2401 ggatggacag gagccaagga tttggttact gtgccgcgcg tgcttcaggt cccgccttga
     2461 tcaccacgga ggagagatcc ggtccacgca ggccggcatc acaaaatggg cccgcaaggg
     2521 cccattttta cgcctcatca acagcgcgca tttcaccgct tccatatctc aaggcgtgaa
     2581 gacgtgccgg aatccatcgt ttgggctagt atgtgctccc ccagcattcg ttgacggcgc
     2641 tccccatgtc acttcctgca tcacccattg tcagcccccg tgccgccgca gcacccggca
     2701 cgccgcgaaa agcggtggcg ctgatctcgg gcgggctcga ttccatgctc gcggccaagg
     2761 tcatgctgga acagggtatt cacgtcgagg gcatcaattt ctacaccggc ttctgcgtcg
     2821 aggggcacac ccacgcgatc cgcaaccacg agcacgccaa gccgaaacgc aacaacgcgc
     2881 tgtggacggc ggagcagctc ggcatcaagc tgcacatcat cgaggtgatc gaagaataca
     2941 aggatgtcgt catcaatccg aagcacggtt acggcgccaa tctcaatccc tgcctcgact
     3001 gcaagggctt catggtgcgc aaggcgcgcg aatggatgga aaaaaacggc ttcgatttca
     3061 tcatcaccgg cgaagtactc ggccagcgcc cgatgagcca gcggcgcgac accatgccgg
     3121 tggtggcgcg cgaatccggc gccttcgacc gcctgctgcg gccgctgtgc gccaagctcc
     3181 tgcccgagac cctgcccgag cgcgagggct gggtggaccg cagcaagctg ctcgacttca
     3241 atggccgcag ccgcaagccg cagatggcgc ttgcagcgca atacggcttc acggattacg
     3301 cccagccggc cggcggctgc tgctttctca ccaacgagca gtactcgcac aaactcgctg
     3361 acctgtggac cacgcgcggc agcaagcagt acgaactcga cgacatcatg ctgctcaagg
     3421 tcgggcggca cctgcgcccg cgcccgaatt tcaaattgat cgtcggacgc gacgagggcg
     3481 agaacaattt tctcaacggc taccgcaagc actttacgca tctctacgcc accagtcatc
     3541 agggcccact ggtgctgatt gacggaaagg ttgatgacac ggacatcgaa ctcgcggcgc
     3601 gcctggccgc gcgcttcggc aagggccgcg aggccgaaac ggtgactctg accgtgaccg
     3661 ataccgcggg caacagccgc gaactccagg tcacgccgat ggcggccaac gacatcccgc
     3721 ccgagtggta tttataagca ataataatat ggcaagacga acggagcgca gcgaagtagg
     3781 gctacccgtc atatgtcagg acgagcgagc gccgcgagca gggctggcgt catatgacag
     3841 gacgaacgaa gcagggcttc ctttatgagc catttcgttc tggacgcacg ctacctgctc
     3901 tgccccatgc cggtgatccg tacccagaac cgaatcgcgg aattgcagcc gggcgacacc
     3961 ctggaagtgc aggccaccga ccccggggcg ttgcacgaca tcccggcctg gtgccgggtg
     4021 cacggacact ccgtgcttgc cactcagcat gaggccaacg aggtgcggat catgcttcgg
     4081 gtaaacaaga catgagcagc gccggcgaac acagccattt cgacatttcc agcccggagc
     4141 gctacaccgc tgcccggcgc gtgacttggg tgagcgtgtt cgtcaacctg ctgcttaccg
     4201 tcgcgcaggt catcatcggc tacctgggca actcgcaggc actggtggcc gacggcattc
     4261 acaccttgtc cgaccttctc accgacttca tggttttgtt tgcgctattc cattcaaaaa
     4321 aggccgccga tgccgaacat ccctacggcc atgagcgcat cgagactgcc accacgctga
     4381 tgctcggcgt catacttttc gccgtgggag ccggcattgc gatacgcgcg gtcatgcgct
     4441 tgctcgaaac caccgcgtta cccacaccgg atttgattac actctgggtt gcccttttca
     4501 cgctcgtcgc caaggagggc ctgttccgtt atctcatgca cacggccaat cgactggatt
     4561 ccaacatgtt gcgcgccaat gcgtggcatt cgcgctcgga cgccgtctcc tccctggtgg
     4621 tagtagttgg catcggtggc agtcgcttcg gtttcaccta cctcgatgcg gtggcggcaa
     4681 tcgtcgttgc tgtcatgatc gtcaaggtcg ctgtcgagct cggttggcaa tcgctgggtg
     4741 aactggtgga cacggggctc gcggcagacg acctcgacgc catccgcaag accatcctct
     4801 cggtcagcgg ggtcaaggcg ctgcacctgc tgcgcacgcg gcgcgtgggc ggacaggcgc
     4861 tggccgacgt gcacatcatc gtggacgatc atgtgagcgt ttccgaggga catcagatca
     4921 gtgaggcagt gcgcaccaag ctgatcaagg aaatcgcgcc gatgacggat gtcatggtgc
     4981 atatcgacac cgaggaagac gtggaaggcc cgagctgcca aggactgccg ctgcgcgacg
     5041 aggtcctgaa gcgtctcgac ggttactttc gcgccatccc cgaggcgcgc cggatcgagc
     5101 atgtcacact gcattatttg aacgggcata ttgatatagg gctgctactg cctctgtcgg
     5161 tcgtagccga tgtctccgcc gcacaagcac tggcccggcg gtttgccgag gctgttcaag
     5221 gcgataagca aatcggtacg atagatgtac aattccactg aataaaataa taaatgcaca
     5281 atattggtgc aaatatttta ttttttgcac tatattagtg cataaaacat taattttatc
     5341 gaagggaatt aattaaatgg ttgattttat ttaaacattt taatggcata aatcgtgcaa
     5401 aattacgagc tcaattttta gttgtgctcg cagcacactt acatttttcc caggaggaaa
     5461 cccatgtcgt ccggtgcaga tgttctcaaa atgatcaaag acaagaaggt caagttcgtt
     5521 gactttcgct ttaccgacac caaaggcaag gaacaacacg tttcggttcc ggcgcacact
     5581 atcgagaccg agaccttcga gcacggcaag atgttcgacg gctcctcgat ctccggctgg
     5641 aagggcattc aggaatccga catgatcctg atgccggaag cagccaccgc ggtgatggat
     5701 ccgttcatgg aggaacctac ccttatactg cgctgcgacg tcatcgagcc cagcaccatg
     5761 cagggctatg aacgcgatcc gcgctcgctc gccaagcgcg ccgaggccta cctcaagagc
     5821 accggcgtgg ccgacaccgc gttcttcggg cccgaggccg aattcttcat tttcgacgac
     5881 gtccgttggg gcgccgacat cagcggttcg ttcgtcaaga tcgactccga agaagcctcc
     5941 tggaacaccg agaaggtgta cgaaggcggc aacctgggcc accgtccgac cgtgaagggc
     6001 ggctacttcc cggttccgcc ggtcgactcg cagcaggaca tccgctcggc catgtgcctg
     6061 gccatggaag aaatgggact ttccatcgaa acccatcacc acgaagtggc gaccgcgggt
     6121 cagaacgaaa tcggcatgct gttcaacacc ttggtgaaga aggccgacga gctccagatc
     6181 tacaaatact gcgtgcacaa tgtggcacac agctacggca agaccgcgac cttcatgccc
     6241 aaacccatcg tcggtgacaa cggctccggc atgcacgtgc accagtcgct cgccaaagat
     6301 ggcaagaacc tgttcagcgg caatgagtac ggcgggctgt cgcagaccgc gctgtattac
     6361 atcggcggca tcatcaagca tgcccgcgcg ctcaacgcca tcaccaattc gctgaccaat
     6421 tcctacaagc gtctggtgcc gggcttcgag gcgccggtca tgctcgccta ctcggcgcgc
     6481 aaccgctcgg cctcgatccg catcccgtac gtgccgaacc ccaagggccg gcgcatcgaa
     6541 gtgcgcttcc cggacgccgg cagcaatcct tatctcgcct tcgccgccat gatgatggcg
     6601 gggctcgatg gcatccagaa caagatccat ccgggcgagg cgatggacaa ggatctgtac
     6661 gatctggaac cggaagagga gaagaagatc ccgaccgtgt gccatgcgct cgatcaggcg
     6721 cttgaggcgc tcgacaagga tcgcgccttc ctcaaggccg gcggcgtgtt caccgacgac
     6781 gtgatcaacg cctatatcga tctcaagatg gccgacgtca cgcgcatgcg catgaccacg
     6841 catccgatcg agttcgacat gtactacagc gtctaagggg cgggcatcag tgcagtaaac
     6901 ccacaatcgc ccccgcctag ggggcgattg tttttgggaa gttaggtatg agatatatct
     6961 ctatgcacca aaatggtgca tgatagatgc tgtaatcagg gaatattgta acttgtttat
     7021 tcataaggaa ttttatagtc gtgaaacatg ttgctctagc ccagtcggct cctgacaggt
     7081 ggcccatgct tacaggatct tccccgcaga ttctcgataa cctgacgacg gcggtgctga
     7141 ccttcgaccc ccgtctgcgt ctgaccagca tcaatcctgc gggcgaaatg ctgttcgaga
     7201 tcagcgccaa gaaggtggtg gggcagtctc tgacggatct gctaccacat gcccggctgg
     7261 cggccaagac gctcacggag acgctggaaa gcagtcatcc tttcacggcc cgcggc
//
LOCUS       DBNGZE010000399         3345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1235615, whole genome shotgun sequence.
ACCESSION   DBNGZE010000399 DBNGZE010000000
VERSION     DBNGZE010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1235615"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..97
                     /locus_tag="AC16FV_10185"
     tRNA            1..97
                     /locus_tag="AC16FV_10185"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Sec,seq:tca)
     gene            123..1220
                     /gene="selD"
                     /locus_tag="AC16FV_10190"
     CDS             123..1220
                     /gene="selD"
                     /locus_tag="AC16FV_10190"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - L-selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR2850460.1"
                     /translation="MSDVRLTSLSHGGGCGCKIAPAALTEILAKVPRPFLDQALLVGI
                     ETSDDAAVYRLNDEQAVVATTDFFMPIVDDPYDFGRIAATNALSDVYAMGARPIFALA
                     IVGMPLDKLPVDVIQEILAGGASVCEAAGIPVAGGHSIDSVEPIYGLVALGVVHPQRV
                     KRNNTARDGDVIVLGKALGVGILSAALKKGALPEEGYRQMLASTTQLNAVGHPLADIP
                     GVHAMTDVTGFGLLGHLAEICRGSRLGARIAFESLPVLPAALSLVQEGYNTGAAARNW
                     SSYGHDVRLPDDLAPWQQALLCDPQTSGGLLVTCAPQAVAKVLSLFRDEGFASAAVIG
                     QMQAGAARITVDYRLGLPAEAQAVQVAEFPG"
     gene            1509..>3345
                     /gene="gyrA"
                     /locus_tag="AC16FV_10195"
     CDS             1509..>3345
                     /gene="gyrA"
                     /locus_tag="AC16FV_10195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR2850461.1"
                     /translation="MEQFAKETIPVNLEDEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELGNDWNKAYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYPM
                     VDGQGNFGSVDGDSPAAMRYTEIRMARIAHEMLADLDKETVDYAPNYDGSEHEPVVLP
                     TKLPNLLVNGSSGIAVGMATNIPPHNLNEVINACVALIDNPDATIDELMKHIPGPDFP
                     TAGIINGVEGIHEAYRTGRGRVYVRARVEIESDDKRGKQSIIVTELPYQVNKALLLER
                     IAELVHEGKIEDIAELRDESDKSGMRMVIDLKRGAVADVILNNLYEHTAMQSVFGINA
                     VALVNGQPRLLNLKELIEAFLRHRREVVTRRSIYDLRKSRERAHILEGLAVALESIDE
                     MIALIKAAKDPDEAKVKMLARTWPAKLVTKMLAAGDADLTRPEGLDKAFGLTPKGYRL
                     TETQAQAILDLRLHRLTGLEQEKLRSEYGDILKIIADLLDILANPERLMKVIRDELTA
                     IGEQYGDKRRTEIVQSRVNLSMEDLIADEDVVVTLSHAGYIKSQPLAAYRAQRRGGRG
                     KTATSMKEEDFVDKLLIANTHATILCFTSLGKVYWMKVYEIPQAGR"
BASE COUNT          666 a         1046 c         1055 g          578 t
ORIGIN      
        1 ggaagagact cgcaccccgg tggggcgacc tgacttcaaa tccggtaagg gccgtcagcc
       61 ggtccttggt gggttcgact cccacgctct tccgccaatt taaccaggca tggaggtgtg
      121 ttatgtctga cgttcgattg accagtttgt cgcacggcgg cggatgcggc tgcaagatcg
      181 cgcccgccgc gctcacggaa atcctcgcca aggtcccacg cccgttcctc gatcaggcgc
      241 tgctcgtggg catcgagacc agcgacgatg ccgcggtata tcgcctgaac gacgagcagg
      301 cggttgtcgc caccaccgat tttttcatgc cgatcgtgga cgacccgtac gatttcggac
      361 gcatcgccgc gaccaacgcg ctgtccgatg tctacgccat gggcgcgcgc ccgatattcg
      421 cgctcgccat cgtcggcatg ccgctcgaca agctgccggt ggacgtgatc caggagatac
      481 tcgcgggcgg agcctcggtc tgcgaagccg cgggcattcc cgtcgccggc ggccactcga
      541 ttgattcggt cgagccgatt tacggcctgg tggcgctcgg cgtggttcat ccgcagcgcg
      601 tcaaacgcaa caacaccgcg cgcgacggcg acgtcattgt gctcggcaag gcgctgggcg
      661 tcgggattct cagcgccgcg ctcaagaagg gcgcgctgcc ggaagaggga taccggcaga
      721 tgctggccag caccacgcaa ctcaacgccg tgggccatcc gctggcggat attcccggcg
      781 tgcatgcgat gaccgacgtc accggcttcg gcctgctcgg ccatctcgcc gagatctgcc
      841 gcggttcgcg gctcggcgcg cgcatcgcgt tcgagtcact gccggtgctc ccggcggcgt
      901 tatcgcttgt gcaggagggt tacaacaccg gcgccgccgc gcgcaactgg tcgagttacg
      961 gccatgacgt gcggctgccg gacgatctcg cgccctggca gcaggcgctc ctgtgcgatc
     1021 cgcagacgag cggcggattg ctggtcacct gtgcgccgca ggcggttgcc aaggtgctgt
     1081 cattgtttcg tgatgagggg ttcgccagcg ccgcggtgat cggacaaatg caggccggcg
     1141 cggcgcgcat caccgtggat taccggctgg gtctgccggc cgaggcgcag gcggtacaag
     1201 tggccgagtt cccgggctga gggcggcccg acgcaatctg ctggagcgat tcgggctacc
     1261 gaaaagatgt gataacgaat cccacccgcg accagatggt cgtgataata caaagtcgtt
     1321 aaaaaccagc ctgtttcaag cgtgtttttc gcgcctctcg cgccgttcaa gcccccggtt
     1381 tttaagtgca gttagggggg taggcatgct agaattcggc gcttctccgg tcccgaaaca
     1441 ggctgaaaaa ataaatactt atcagttact tatcaatgag ttatccacga tagggtaact
     1501 gatttcttat ggaacaattc gccaaagaaa ccattccggt caatctcgaa gacgagatgc
     1561 gacggtccta cctcgattac gccatgagcg tgatcgtggg ccgggcgctg ccggacgtgc
     1621 gcgacggtct caagccggtg caccggcgtg tgttgttcgc catgcacgag ctcggcaacg
     1681 actggaacaa ggcctacaag aaatccgcgc gcgtggtcgg cgacgttatc ggtaaatacc
     1741 acccgcacgg cgacaccgcg gtgtacgaca ccatcgtccg gatggcccag gacttttcgc
     1801 tccgttaccc gatggtggac ggtcagggga acttcggttc cgtagatggc gactccccgg
     1861 cggccatgcg ctacaccgaa atccgcatgg cgcgcatcgc gcacgagatg ctggccgacc
     1921 tcgacaagga aaccgttgac tacgccccca actacgacgg ctcggaacac gagccggtgg
     1981 tgctgccgac caagctgccg aacctgctgg tcaacggttc ctcgggcatc gccgtcggca
     2041 tggcgaccaa catcccgccg cacaatctga atgaagtcat caacgcctgc gtcgcgctca
     2101 tcgacaaccc ggacgcgacc atcgacgagc tgatgaagca catccccggt ccggattttc
     2161 cgaccgcggg catcatcaac ggcgtcgagg gcatccacga ggcctatcgc accgggcgcg
     2221 ggcgcgtgta cgtgcgcgca agagtggaga tcgaatccga cgacaagcgc ggtaaacaat
     2281 ccatcatcgt caccgagctg ccgtaccagg tgaataaggc gctgctgctg gaacgcatcg
     2341 ccgagctggt gcacgagggc aagatcgagg acatcgccga gctgcgcgac gagtcggaca
     2401 agtccggcat gcgcatggtc atcgatctca agcgcggcgc ggtcgccgac gtgatcctga
     2461 acaatctgta cgaacacacc gcgatgcaga gtgtgtttgg cattaacgcg gtggcgctgg
     2521 tcaacggcca gccgcgcctg ctcaacctca aggaactgat agaggccttc ctgcgccacc
     2581 ggcgcgaagt ggtcacgcgg cgttcgatct atgacttgcg caagtcgcgc gagcgcgcgc
     2641 atattttgga aggcctcgcg gtcgcactcg aaagcatcga cgagatgatc gcgctcatca
     2701 aggccgccaa agacccggac gaagcgaaag tgaaaatgct ggcgcgcacc tggccggcga
     2761 aactggtaac caaaatgctg gcggccggcg acgccgacct gacgcggccg gagggactgg
     2821 acaaggcctt cggcttgacg cccaagggct atcgtctgac ggagacacag gcgcaggcga
     2881 tcctcgatct gcggctgcac cgcctgaccg gactcgagca ggaaaaactg cgcagtgaat
     2941 atggcgacat tctcaagatc atcgccgacc tgctcgacat cctcgccaac cccgagcgcc
     3001 tgatgaaggt gatccgcgac gagctgaccg cgatcggcga gcagtatggc gacaaacgcc
     3061 gcaccgagat cgtgcagagc cgcgtaaatc tcagcatgga agacctgatc gccgacgagg
     3121 atgtggtcgt gaccctgtcg catgccggtt atatcaagtc gcagccgctg gcggcttacc
     3181 gcgcacagcg ccgcggcggg cgcggcaaga ccgcgaccag catgaaggaa gaggatttcg
     3241 tcgacaagct gctgatcgcc aacacccacg ccacgatcct gtgctttacc agcctcggca
     3301 aggtgtactg gatgaaggtg tacgagattc cgcaggctgg gcgcg
//
LOCUS       DBNGZE010000400         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_542697, whole genome shotgun sequence.
ACCESSION   DBNGZE010000400 DBNGZE010000000
VERSION     DBNGZE010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_542697"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16FV_10200"
     CDS             <1..609
                     /locus_tag="AC16FV_10200"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypE family hydrogenase expression/formation
                     protein"
                     /protein_id="HIR2850462.1"
                     /translation="PRGEGGGLYLATTGVGVRRKNTRLDMQQIRDGDVILVSGPVGDH
                     GIAVMLAREQFGMRGDLQSDCASVLPLTQALLPFDGLRFMRDPTRGGLAMIAHEIRRA
                     TGMGIYMDEAAIPMREAVESVCELLGYDPLYLACEGRVVAVVAPDQAQAALAAWRVLP
                     QGQEAALIGKIDKTRRFVVMQTALGGERMLDELEDDPLPRIC"
     gene            complement(641..1174)
                     /locus_tag="AC16FV_10205"
     CDS             complement(641..1174)
                     /locus_tag="AC16FV_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850463.1"
                     /translation="MENGMIAGRCARNSIITLLMMCFFGFFGISANAGDFSVLVNGKA
                     IHLDTQPGVHYNEKNWGLGFQYDMAPVEEKWVPFVTASEFKDSNENTSYYFGGGFLHR
                     TSFEWGKTPMHFDAGGIAFLMKRKGFKDGDLFPGILPAVSLGTSRVAVNMTFIPKVDP
                     KMVPILFFQLKITLGNF"
     regulatory      complement(1325..1413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1567..>2231)
                     /locus_tag="AC16FV_10210"
     CDS             complement(1567..>2231)
                     /locus_tag="AC16FV_10210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR2850464.1"
                     /translation="SLSAVYDRRRDTVGHYVAVFNDISLYKANEERLKFLATHDALTG
                     LPNRAEFKRYCHEAIERARRSRRRVAVLYIDLDNFKPVNDTYGHLAGDELLKTVGARM
                     KQVMRETDIVARIGGDEFSVLLADLDDISSGYIVACKLLDMLSQPVETRHGKHVVGAS
                     IGVSTYPDDGDDPQALLRLADAAMYRVKQGGRNGVGFYSAPAGLPETVAPGTDRGLAT
                     KR"
BASE COUNT          435 a          674 c          699 g          423 t
ORIGIN      
        1 ccgcgcggcg aaggcggcgg gctttatctc gcgaccaccg gcgtcggcgt gcgtcggaaa
       61 aacacccggc tcgacatgca gcagatacgc gatggcgacg tcattctcgt cagcgggccg
      121 gtgggcgatc acggcatcgc ggtgatgctg gcgcgcgagc aattcggcat gcgtggtgac
      181 ctgcaatccg attgcgccag cgtgctgccg ctgacacagg cgctgttgcc tttcgacggg
      241 ttacgcttca tgcgggatcc cacgcgcggc gggttggcca tgatcgcgca cgaaatccgc
      301 cgcgccacgg gcatgggcat ctacatggac gaagcggcga ttccgatgcg cgaggcagtg
      361 gaatcggtgt gtgaattgct cggttacgat ccgctgtatc tcgcctgcga gggccgggtg
      421 gtggcggtgg tggcgcctga tcaggcgcaa gcggcgctgg ccgcgtggcg cgtcctgccg
      481 cagggacagg aagcggccct gatcggcaag atcgataaaa ccaggcgctt tgtggtgatg
      541 cagacagcgc tcgggggcga gcgcatgctg gatgaactgg aggacgaccc gctgccgcgg
      601 atctgctagg gcgccgccgt gacggcacgc cagggaagag ctagaagttg ccgagcgtga
      661 tcttgagctg gaagaacagt atcggcacca tcttggggtc gaccttgggg ataaaggtca
      721 tgttgaccgc gacccgcgac gtgcccagcg acaccgccgg cagaataccc gggaacaggt
      781 caccatcctt gaagcccttg cgtttcatga ggaaggcaat cccgccggca tcgaaatgca
      841 tcggggtctt gccccactcg aagctggtac ggtgcaaaaa tccgccaccg aaataataag
      901 acgtgttttc attcgagtcc ttgaattctg aggccgtgac gaacggcacc catttttcct
      961 caaccggcgc catgtcgtac tggaaaccca gaccccagtt tttctcgttg tagtggacac
     1021 cgggctgagt atcgagatgg atcgctttgc cgttcaccag cacgctgaaa tcaccagcat
     1081 tcgcggaaat accgaagaac ccgaagaaac acatcatgag cagcgtgatg atcgagttcc
     1141 gtgcacaccg tccagcaatc attccgtttt ccattgccat tacccccaaa tcttggccgg
     1201 gcaaggtttc cggaacggcg gggcgacaac acaggtgggc gtgaggcgag gttacggaag
     1261 acggcagccg actatgcgga acagcgttgt ggccctgcct gcttccttgc ctggaatgtt
     1321 gagttccggc aatcccgctg tcatagggcc catgcccctt agaccggtaa ctttgcgtcc
     1381 tccgcctttc ggccgaggta gccatttaca gaacttgcaa cccatgacag cgtccttccc
     1441 tgtcatgcgc tggatataaa gcaactagcg tgccagcggg acgccggcgg gaaagccgcg
     1501 ggttctcagc ggattctgcg gattttcagg cggggagaac gggatggata ctgccgaggt
     1561 gacaattcag cgcttggtgg caagccctcg atccgttccc ggggccacag tttcaggcaa
     1621 tcccgctggc gcggaataga agccgacacc gttgcgaccg ccctgcttca cccggtacat
     1681 ggcggcgtcg gccagccgca acagggcctg cggatcgtcg ccgtcatccg gataggtgct
     1741 gaccccgata ctggccccga cgacatgctt cccgtgacgg gtctcgaccg gctgcgacag
     1801 catgtcgagc agcttgcacg ccacgatata gccgctcgaa atatcatcga gatcggccaa
     1861 caaaacactg aattcatcgc caccgatgcg tgccacgatg tccgtctcgc gcatcacctg
     1921 tttcatgcgg gcaccgacgg ttttcagcag ctcgtcgccg gccagatgtc cgtaggtatc
     1981 gttgacgggc ttgaagttgt cgaggtcgat gtacagtacc gccacccgcc gtcgggaacg
     2041 ccgcgcgcgc tcgatcgcct cgtggcaata acgcttgaac tccgcccggt tcggcaggcc
     2101 ggtcagcgca tcgtgggtcg cgaggaattt gagccgttcc tcgttggcct tgtacagcga
     2161 gatgtcgttg aacaccgcga cgtaatggcc gaccgtatcg cgccgccggt cgtacaccgc
     2221 gctcaggctc a
//
LOCUS       DBNGZE010000401         2149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_125088, whole genome shotgun sequence.
ACCESSION   DBNGZE010000401 DBNGZE010000000
VERSION     DBNGZE010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_125088"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16FV_10215"
     CDS             complement(<1..611)
                     /locus_tag="AC16FV_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850465.1"
                     /translation="MGLTCLFAASIVLSAASAHAQAFVPPSGAIADYQAKLAVYEQVH
                     GAYEAQASAYWDAISEKRRTRNGKRRDHLPIGLDDYVVTQPPVYSGPPRPVDPRAPAA
                     PTPERPEIPVVADFLKNAVEQFGFVPDRPASDLDFKRAYARIALAAGLTREQIVGIYA
                     FETGGNGAYDSQAGLVPYRPGARAISPAVGYNQLLSTNTVSLLA"
     gene            complement(741..950)
                     /locus_tag="AC16FV_10220"
     CDS             complement(741..950)
                     /locus_tag="AC16FV_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850466.1"
                     /translation="MNWSMYTADRTTHLKIVVVGLVAALMIAVIGISARELNLGTDIM
                     TAQTPAVIKAGGPVVFTDRSGPTVR"
     gene            complement(1455..1775)
                     /locus_tag="AC16FV_10225"
                     /pseudo
     CDS             complement(1455..1775)
                     /locus_tag="AC16FV_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2005..2148)
                     /locus_tag="AC16FV_10230"
     CDS             complement(2005..2148)
                     /locus_tag="AC16FV_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HIR2850467.1"
                     /translation="MRGGLLSLEEACARYTLTVEEFLSWQYSIDQHGLAGLRTTRIKQ
                     YRQ"
BASE COUNT          418 a          603 c          696 g          432 t
ORIGIN      
        1 gccaagagcg acacggtgtt ggtgctgagc aactggttgt aacccaccgc cggcgaaatc
       61 gcgcgcgcgc ccggccggta gggtacgagg cctgcctgtg agtcgtaggc gccgttgccg
      121 ccggtctcga aggcatagat gccgacgatc tgttcgcgcg tgagtccagc ggcgagggcg
      181 atgcgggcat aagcgcgctt gaagtcgagg tcactcgccg gccggtccgg cacaaagccg
      241 aattgttcga cagcgttctt cagaaaatcg gcgacgacgg gaatttccgg ccgctccggc
      301 gttggcgcag caggcgcacg cggatcgacc ggccgcggcg gcccggaata gacaggcggc
      361 tgggtcacca cgtagtcgtc gagcccgatc ggcagatggt cgcggcgctt gccgttgcgg
      421 gtacggcgct tttcggagat tgcgtcccaa taggcggaag cttgcgcttc ataggcgcca
      481 tgcacctgtt catagaccgc gagcttggcc tggtagtcag ctatcgctcc gctcggtggc
      541 acgaatgctt gagcatgcgc cgacgctgca gacagcacta tgctggcggc gaacaggcag
      601 gtaaggccca aatggagtga acagttcttc atgcttgatt tgttgtcggc cattttgctt
      661 gattcgacca cttcacaata aaacgcgccg caaatgcggc gcgcgaggag gggaaggttc
      721 ggggatcgtt ggtaaacggc ttaccggacg gtcgggccgc tgcggtctgt aaagaccacg
      781 gggccgccgg ctttgatgac agccggggtt tgcgcggtca tgatatccgt gccgagattg
      841 agttcgcgcg ccgagatgcc gatgacggcg atcatcagcg cggcgacaag cccgaccacc
      901 acaatcttga ggtgggtagt gcggtctgcg gtatacatgg accagttcat gacgtcctcc
      961 ggggctcttg cacgttggtc gcccggtcgc gacaaaatgt tcatctagtt cctgtcacca
     1021 acgtagttac ggcatcccgg ttcccaagga cccatcacgg atcattgatt cgctgtgata
     1081 tcagccaatt tttcgcagtt ttacaggcgc tctagcgcgg tttcggtcac gcgaccttcg
     1141 ccgggggacc ggcccagggc acagatgtct agatgctgct gacggttttg agatgcgcgc
     1201 gggcatgaat ttccacttgc gagagaacag gcgtctgtcc ccgaagcgct cgacgatacc
     1261 gcgaatggat ggccggatat tttatgaggt catcaggacc agcacctcgc cttcgcggtc
     1321 aacttactcg aaacgttcgc gaacgagcgt gataaatgcc gcggcccgat aagcgtgcgc
     1381 acgtgttcgg agagcgttac cggctagcgc gagcaagcga tgctgtcggc aatccttcga
     1441 ggaggtgatg agatttaagc agcgcccctg agctccgggc ccccatgggg cggcggaatt
     1501 ggtgaaccgt cggcagcata tacattgagc ttgttgcgca acgtacggat cgagatgccg
     1561 agaatgtttg cggcatgggt gcggttgcca aggcagtgct tgagcgtctc aaggatcagg
     1621 tcgcgctcga cttcggccac tgtgcggcca accagattgt aggcgacttg ttcggcagcc
     1681 aaagccgcat ggggaatgtc ggtgtggtgc gcttggccga gccacgcacc atctggcgac
     1741 agaatgtcgt cgacaccaat ttccgcgccg gtcgagggtc gcatcagttg tgctccgcct
     1801 tgatgatttc cgtcatggtc gcgctgagct tatcttcccc caggatgact tcacacttca
     1861 ctcggcaaca tttgcccagt ttaaggttag cgtccggtta acgcctgcaa ttgtgcaaaa
     1921 gaaatacacg gtcgcggcag ggccgacgcg aatgaaatta ctggcgcgct ggcgccggcg
     1981 ttcacgagca gacgggggac gaacttactg gcggtactgt ttaatgcgtg tcgtgcgcag
     2041 gccagcgagg ccgtgctggt cgatcgagta ctgccaggag aggaattcct cgaccgtaag
     2101 ggtgtaacgc gcgcaggctt cctcaaggga caaaagcccg ccacgcacc
//
LOCUS       DBNGZE010000402         6929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_55709, whole genome shotgun sequence.
ACCESSION   DBNGZE010000402 DBNGZE010000000
VERSION     DBNGZE010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_55709"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16FV_10235"
     CDS             complement(<1..723)
                     /locus_tag="AC16FV_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR2850468.1"
                     /translation="MSPLTLAYLALFSALASGVLALGSERRANLLRYGSSLLLGSCGL
                     LAIVAGAWAMIDEAPTVDRLALGLPWLQWHLRLDPLSGFFFGVIGLLVFAVALFAPGY
                     LREFTHHGHRQGISALGLFTGLFILGMLLVLLADDAFVFMISWELMSVSSYFLVAYQH
                     QHAANRRAAFLYLLMAHIGALAILLAFGILAAFGGGLTFEQMRAAELTPLWATIAFVT
                     ALVGFGTKAGIVPLHAWLPEAHP"
     gene            complement(970..2259)
                     /locus_tag="AC16FV_10240"
     CDS             complement(970..2259)
                     /locus_tag="AC16FV_10240"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HIR2850469.1"
                     /translation="MIVRIDTQQLRIGMYVCELDRPWRETPFLFQGFEIRTDEELKTL
                     QQYCRSVFIVANDAATLALAKRAPIRPDLLKPTADAESGKKSAISVEQEMLKLNNHPG
                     ASSVYTDETTLEEEIQNIKDIHAGACELIQELMWAAKFGRSLDVPGARQTLSRMVESV
                     LRNPDALTCFAQLKKKDEYTAQHSLRVAILSLVFGRQLGLTPADLEMLGLGALLLDVG
                     KMKIPSEILNKPASLTGDELALVRQHVPWGIEILEKTDQIPAMALEVARRHHERYDGS
                     GYGDKLKGDAIGTFGMIAGILDYYDAVTSDRVYRAGISAHAALKKMYEGRSTLFHPEL
                     TEKFIQCLGIYPIGSVVELNTGEIGVVAALNRKQRLKPRVVLVQHADKTPYPDSPVVS
                     LANRKNPDGRACEIERVLDPAECGIDPARYLPLTGTA"
     gene            2651..3067
                     /locus_tag="AC16FV_10245"
     CDS             2651..3067
                     /locus_tag="AC16FV_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR2850470.1"
                     /translation="MRFMIIVKATKDSEAGVMPEEKLLTDMANFHEELQKAGVLLDGS
                     GLQPSKKGWRIKYSGTKRTVVDGPFTETKELIAGYTLIQVKSREEAMEWTRRFPNPAI
                     GGKEGEIEVRQLFELEDFGPSETIERFREMGVGTQK"
     gene            3112..3525
                     /locus_tag="AC16FV_10250"
     CDS             3112..3525
                     /locus_tag="AC16FV_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2850471.1"
                     /translation="MRVEPYLFFDGRCDESIEFYRHVLGAEVTMLMRYKESPEPSQPG
                     MIPPGSENKVMHSNLRIGETMVMASDGRCSGKPSFQGFSLSVTAPNETEAERMFAALA
                     GGGQVQMPLAKTFFSPRFGMVADRFGVSWMIIVTP"
     gene            3539..4024
                     /locus_tag="AC16FV_10255"
     CDS             3539..4024
                     /locus_tag="AC16FV_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR2850472.1"
                     /translation="MRMEYNGFPRQDSQRALILMRVFNAPRELVWKAWTDPTLTQRWM
                     GPRGFSATHLASDLRPRGIWRVCLRRDDNGEEYWQGGVYLEIVAPEWLTYSFAWDDTR
                     ERRNHVTRVALSFTERRRKTTMSFRQEMFETVAERDGHLLGWNSTFDRLAELLAEIQR
                     K"
     gene            4041..4397
                     /locus_tag="AC16FV_10260"
     CDS             4041..4397
                     /locus_tag="AC16FV_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR2850473.1"
                     /translation="MAYLLLIVEPRGQRAERTQEEGRDAYAQMVRFGEGLKARGLLKA
                     VESLQSDATGVRVSVRGGKRKLLDGPFAEAKEMVGGFFLIDNVTREQAIEIAGECPAA
                     AWATVEVREVGPCFTG"
     gene            4481..5005
                     /locus_tag="AC16FV_10265"
     CDS             4481..5005
                     /locus_tag="AC16FV_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2850474.1"
                     /translation="MLKTILIVVVLLIAAVLIYAAIQPDTFRVERSVSIKAPPEKIFP
                     LIDDLHRMQTWSAWEKVDPGMKRAYSGGASGKGAVYEWEGNKDIGQGRMEIIESSPSR
                     VTIRMNFIKPFAAQNTLEFTLKAEGGSTQVTQAIFGPRPYISKLFGIFCSMDKMIGGK
                     FEESLTALKSAAEA"
     gene            5024..5512
                     /locus_tag="AC16FV_10270"
     CDS             5024..5512
                     /locus_tag="AC16FV_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2850475.1"
                     /translation="MQKITPFLWFDNQAEEAANFYTSIFKNSKIVNVARYGEEGAKVS
                     GRPKGTAMTVAFQLDAQEFVALNGGPHFKFTEAVSFVVNCKTQQEVDHYWEKLSAGGD
                     EKAQQCGWLKDKYGLSWQIVPDVLGKMLQDKDPKKSERVMAALLKMKKLDIKALKQAY
                     EQ"
     gene            5604..5963
                     /locus_tag="AC16FV_10275"
     CDS             5604..5963
                     /locus_tag="AC16FV_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HIR2850476.1"
                     /translation="MNYVDGFVVPVPTKNLAAYRRMASLAGKVWREHGALEYVECVAD
                     DVKPGKYTSFPQSVKLKRGEVVVFSWIVYKSRKDRDRVNAKVMKDPRIAPMMDVKALP
                     FDGKRMFWGGFKEIVDL"
     gene            6071..>6929
                     /locus_tag="AC16FV_10280"
     CDS             6071..>6929
                     /locus_tag="AC16FV_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2850477.1"
                     /translation="MTTTDTHRTIDAVWRIESARIIAKLARMVRDVGLAEELAQDALV
                     AALEHWPEAGIPDNPGAWLMATAKHRAIDRLRRNKLLERKHEELGHELELQQEMAEPD
                     LDAAINDDVGDDLLRLMFTACHPVLSTEARVALTLRLLGGLTTDEIARAFLVPEPTIA
                     QRIVRAKRTLAEARIPFEVPRGDELVARLSSVLEVIYLVFNEGYSVTAGEDWMRPALC
                     QDALRLGRVLAELAPNEPEVHGLVALMEIQASRAGARVGPKGEPILLLDQNRARWDRL
                     LIRRGLAALE"
BASE COUNT         1460 a         2088 c         2152 g         1229 t
ORIGIN      
        1 cgggtgcgcc tcgggcagcc aggcgtgcag cggcacgatg ccggccttgg tgccgaagcc
       61 cacgagcgcg gtgacgaagg caatcgtcgc ccacagcggc gtcagctccg cggcgcgcat
      121 ctgctcgaac gtgaggccgc cgccgaaggc ggcgaggatg ccgaacgcca gcagaatcgc
      181 gagcgcacca atatgcgcca ttaataagta caggaacgcc gcgcgccggt tggcggcgtg
      241 ctgatgctga tacgcgacca gaaaataact cgagaccgac atcagctccc acgagatcat
      301 gaagacgaag gcgtcgtccg cgagcagcac cagcagcatg ccgagaatga acaggccggt
      361 gaacaggccg agcgccgaaa tcccttgccg gtgtccgtgg tgagtgaatt cccgcaggta
      421 gccgggggcg aacagcgcga cggcgaaaac cagcaagccg atcacgccga agaaaaaacc
      481 ggacagtggg tcgagccgca gatgccactg gagccacggc aggccgagcg ccaaccggtc
      541 caccgtgggc gcctcgtcga tcatggccca ggctccggcc acgatggcca gcaggccaca
      601 cgatccgagc aggagggacg aaccgtagcg cagcaggttc gcgcgacgct ccgaacccag
      661 cgccaaaacc ccggaggcca gggcgctgaa aagagccaga taggcaagtg ttagaggcga
      721 caagcttttt gaaccggttg gacggcgcgg atgctcatgc gggtggccgc tgcctattac
      781 tattaatagt tagttgctca ggctggggaa gcgccgctga tgctagagcc tgggttggga
      841 aaattcaagc agcgccggta aaaacgcggt caacggcacg ccgtcgatcg gacccggact
      901 ggaacggctc agacagccac tcggctgagg caccggcggc tacgcggaag cggcgctatc
      961 gccgttgtct caggcggtgc ctgtaagagg aaggtaacgc gcgggatcga tgccgcactc
     1021 ggcgggatcg agcacgcgtt cgatttcgca ggcgcgcccg tcgggattct tgcggttggc
     1081 gagactgacg accggcgaat cgggatacgg ggtcttgtcg gcgtgctgca ccagcaccac
     1141 gcgcggtttc agccgctgct tgcggttgag cgccgccacc acgccgatct cgccggtgtt
     1201 gagctcgacc acgctgccga tcggataaat gcccaggcac tgaatgaatt tctcggtcag
     1261 ctccggatga aacagggtgc tgcgtccctc gtacattttc ttcagggccg cgtgcgccga
     1321 gatgccggcg cgataaacgc ggtcgctggt aacggcgtcg tagtaatcga gaatgccggc
     1381 aatcatgccg aaggttccga tggcgtcacc cttgagcttg tctccgtagc cgctgccgtc
     1441 gtaacgctcg tggtggcgcc gcgcgacttc cagcgccatg gcaggaatct gatcggtctt
     1501 ttcgagtatt tcgatgcccc atggcacatg ctgtcgcacc agcgccagtt catcgcccgt
     1561 gagcgatgcc ggcttgttca gaatttcgct cggaatcttc atcttgccga cgtcgagcaa
     1621 cagggcgccg aggccgagca tctccaggtc cgcgggtgtc agacccagct ggcgcccgaa
     1681 cacgagcgac aggatggcca cgcgcaggct gtgctgcgcg gtgtattcat ctttcttctt
     1741 cagctgcgcg aagcaggtca gcgcgtcggg attgcgcagc acgctctcca ccatgcggct
     1801 gagggtctgg cgcgcgccgg gcacatcgag gctgcggccg aatttcgccg cccacatcag
     1861 ctcctggatc agttcgcagg ctccggcgtg gatatccttg atgttctgga tttcctcctc
     1921 gagggtggtc tcgtccgtgt acacggaact ggcgccggga tgattgttaa gtttcagcat
     1981 ttcttgttcg accgaaatag cggacttctt cccggactca gcatccgccg tgggcttgag
     2041 cagatcggga cggatgggcg cacgcttcgc gagcgccaaa gtagcagcgt cgtttgccac
     2101 gatgaagaca ctgcggcagt actgctgcag tgtcttcagt tcctcgtccg tgcggatctc
     2161 gaaaccctga aacagaaacg gcgtctcgcg ccacgggcgg tcgagctcgc aaacgtacat
     2221 cccgatcctg agctgctgtg tgtcaatcct gacgatcatc ttggctccac tgtgtcctta
     2281 cgaccgctac gcccctggcg ccggcacaaa cccgaccggc accccggtac cccgaacttg
     2341 tccggatact cataaatcaa tgcaaaaatc ataccgatca agcaattatc aaaaggaggg
     2401 gtaagtaccg ggggtcctga aaaccgagcc gggcaaacag acgcagatta tgaaaaaagt
     2461 ggcatcagat gccacttttc agcagggggg agaaaaacac atgccgcaat gtcaaagtga
     2521 acgccccgat cagcccctgt tgaagccgcg cgattgggcc tgtcgatttc cccgttcctc
     2581 gttcgtcgta cagtaaagcg ccataaaccc ggcgctggac ccgaaaccat catcataagg
     2641 aggcagaacc atgcgattca tgattattgt caaagccacc aaagactccg aggcgggcgt
     2701 catgccggag gagaaactcc tcaccgacat ggcgaacttc cacgaggagt tgcagaaggc
     2761 cggagtgctg ctcgacggct ccgggctgca accgagcaag aagggttggc gcatcaagta
     2821 ttccggcacg aagcgcaccg tggtcgacgg gcccttcacc gagaccaagg agttgatcgc
     2881 cggctacacg ctgattcagg tgaagtcgcg ggaagaagcg atggaatgga cccggcgctt
     2941 ccccaatccc gccatcggcg gcaaggaagg cgagatcgaa gtgcgccagc tgttcgagct
     3001 ggaggacttc ggtcccagcg agacgataga gcgctttcgc gagatgggag ttggaacgca
     3061 gaagtaatgg ccgcgcgcca cgcttcaaaa ccgcaaccac aaggagacac catgcgagtc
     3121 gaaccctatc tgttctttga cggccgctgc gacgagtcga tcgagtttta tcgccatgtg
     3181 ctcggcgcgg aggtgaccat gctcatgcgt tacaaggaga gccctgaacc ctcgcaaccc
     3241 ggcatgatcc cgccgggctc cgaaaacaag gtgatgcatt cgaaccttcg tatcggcgag
     3301 acgatggtaa tggcttccga cggccgctgc tcgggaaagc cgagcttcca gggtttttca
     3361 ctgtcggtaa cggcaccgaa cgagaccgag gccgagcgca tgttcgccgc gctcgccggc
     3421 ggcgggcagg tgcaaatgcc cttggccaag accttcttct ctccgcgctt cggcatggtc
     3481 gccgaccgct tcggcgtgtc gtggatgatc atcgtgacac cataagaggg gaaacaggat
     3541 gcggatggaa tacaacggtt tcccgcgcca ggattcgcag cgcgccctca tcctcatgcg
     3601 ggtttttaat gcgccgcgcg agctggtttg gaaggcctgg accgacccga cactcacgca
     3661 gcgctggatg ggcccgcgcg gtttcagcgc cacacatctg gccagcgatt tgcggccccg
     3721 cggtatatgg cgcgtgtgcc tgcggcgcga cgacaacggc gaggaatact ggcaaggcgg
     3781 agtctatctc gagattgtcg caccggaatg gctcacctac tctttcgcct gggacgacac
     3841 ccgtgagcgt cgtaaccatg tcacccgggt cgccctgagc ttcaccgaac gccggagaaa
     3901 gacgacaatg agtttccgcc aggagatgtt cgagaccgtc gccgaacgtg atgggcatct
     3961 gctcggatgg aacagcacct tcgaccgcct cgcggaactg ctcgcggaaa tccaacgcaa
     4021 atgaacccaa aggaaatcac atggcttatc tgctcttgat cgtggaaccc cggggccaac
     4081 gcgccgaacg cacgcaggaa gaaggacgag acgcgtacgc gcagatggtc cgcttcggcg
     4141 agggcctcaa ggcgcgcggc ctgctcaagg ccgtggaatc cctgcagtcc gacgccaccg
     4201 gcgtgcgcgt gtcggtgcgc ggcggcaagc gcaagctgct cgacggccct ttcgccgaag
     4261 ccaaggaaat ggtcggcggc ttctttctca tcgacaatgt gacccgggaa caggcgattg
     4321 agatcgccgg cgaatgcccc gcggccgcgt gggccaccgt cgaggtgcgc gaggtcggtc
     4381 cctgttttac cggctgaata taaatggcgc tccgtgtgtc gatttcgccc ggtttcattc
     4441 gtcgtacgga tagcaattca actctaataa ggagaaacac atgctcaaga ccattcttat
     4501 cgttgtcgtc ctgctgatcg cggccgtgct gatctatgcc gcgatccaac cggacacgtt
     4561 ccgcgtcgag cgatccgtca gcatcaaggc gccgccggaa aagatttttc ctctgatcga
     4621 cgacctgcac cgcatgcaaa cctggtcggc ctgggaaaaa gtggatcccg gaatgaaacg
     4681 agcctacagc ggcggcgcca gcggcaaggg cgccgtctac gagtgggagg gcaacaagga
     4741 catcggccag ggacgcatgg aaatcatcga gtcgtcgcca tcgcgggtta ctatccggat
     4801 gaatttcatc aagccgttcg ccgctcaaaa cactttggag ttcacactaa aagccgaggg
     4861 cggttcgacg caggtcacgc aggcgatctt cggtccgcgt ccctatatct cgaagttgtt
     4921 cgggatcttt tgcagcatgg acaagatgat cggcgggaaa ttcgaagaaa gcctgaccgc
     4981 gctgaagtcc gcggccgaag cgtgagacaa ggagtaaaac gtcatgcaaa agatcacccc
     5041 gttcctgtgg ttcgataacc aggccgagga ggcagcgaat ttttatacct ccattttcaa
     5101 gaattcaaag atcgtgaacg ttgcccgcta tggcgaagaa ggggcaaagg tctccggcag
     5161 acccaaggga acggccatga ccgttgcctt ccagctcgat gcgcaggaat ttgtggcgct
     5221 gaacggagga cctcacttca aattcaccga agccgtatcg tttgtcgtga actgcaagac
     5281 ccagcaagaa gtggaccatt actgggagaa actctccgcg ggcggggatg aaaaggcgca
     5341 gcaatgcggc tggctgaaag acaaatacgg cctgtcgtgg cagatcgttc ccgatgttct
     5401 gggcaagatg ttgcaggaca aagacccgaa gaaatcggag cgggtcatgg cagcattgct
     5461 caagatgaaa aaactggaca tcaaggcatt gaagcaggcg tatgagcaat gacccatgac
     5521 ggtttaatga cgacaactgt ggtagctgac aaggagtact taatatgact agacgaatga
     5581 gtgtagcgag cagggcttct catctgaatt acgtagacgg ctttgtagta ccggtcccca
     5641 ccaagaacct ggcggcgtat cgccgcatgg ccagcctggc gggaaaagtc tggcgcgagc
     5701 atggcgcgct cgaatacgtc gaatgcgtcg ccgatgacgt gaaaccgggc aagtacacgt
     5761 cgttcccgca gagcgttaag ctcaagcgcg gcgaggtcgt ggtgttctcg tggatcgtgt
     5821 acaagtcgcg caaggaccgc gatcgcgtca acgccaaggt catgaaggac ccgcgcatcg
     5881 cccccatgat ggatgtcaaa gccctgccct tcgacggcaa gcgcatgttc tggggcggat
     5941 tcaaggagat cgtggatctc tgagcagtac gcgcccgggc gcgcagtgag cgtgctccgc
     6001 tcaggtcatc cgtagcgaat ccctgcttcg ctacgctcct tcttcttgct tgttgtatcc
     6061 tcgacccacc gtgaccacaa ccgacaccca tcgcaccatc gacgcggtct ggcgcatcga
     6121 gtccgcccgg atcattgcca agcttgcgcg catggtgcgc gacgtcggtc tggccgagga
     6181 acttgcgcag gacgcgctcg tcgccgcgct cgaacactgg cccgaggcgg gcatcccgga
     6241 caatcccggg gcctggctca tggccaccgc gaagcatcgc gccattgaca ggttgcgccg
     6301 caacaagctg ctcgaacgca agcatgaaga actcggtcac gaactcgaac tccagcagga
     6361 aatggccgag ccggatctcg atgccgcgat caacgatgac gtcggcgatg acctgctgcg
     6421 cctgatgttc acggcctgcc atccggtgct ctccaccgag gcgcgcgtgg cgctcacgtt
     6481 gcgcctgctc ggcggcctga cgaccgacga aatcgcgcgc gccttcctcg tcccggaacc
     6541 gaccatcgcc cagcgcatcg tgcgggccaa gcgcacgctc gccgaggcgc gcatcccctt
     6601 tgaggtcccg cgcggggacg aactcgtcgc ccgcctgtcg tccgtgcttg aggtgattta
     6661 tctcgtgttc aacgagggct actcggtgac cgccggcgag gactggatgc ggccggcgct
     6721 gtgccaggac gcgctgcgtc tcgggcgtgt cctggccgaa ctcgcgccaa acgaacccga
     6781 ggtccacggc ctcgtcgcgc tgatggaaat ccaggcgtcg cgcgcgggcg cgcgcgtcgg
     6841 accgaagggc gagcccatcc tgctgctcga ccagaaccgc gcgcgctggg atcggctttt
     6901 gatccgccgc ggtcttgcgg cgctggaac
//
LOCUS       DBNGZE010000403         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1514670, whole genome shotgun sequence.
ACCESSION   DBNGZE010000403 DBNGZE010000000
VERSION     DBNGZE010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1514670"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..701)
                     /locus_tag="AC16FV_10285"
     CDS             complement(294..701)
                     /locus_tag="AC16FV_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850478.1"
                     /translation="MRLYKCAFSNTLITNQFGCEKAEQVTRRGGPDIACTSDVAHQRC
                     EKLFQQMKAAALPAFGVEDDLLSMPHSVLVKIQYGGLLGLQRLLDSAVATGDVKNVYA
                     LVDQAIGKYNGLDTVPFPALVQDMTSYKLRRRG"
     gene            complement(735..1145)
                     /locus_tag="AC16FV_10290"
     CDS             complement(735..1145)
                     /locus_tag="AC16FV_10290"
                     /inference="COORDINATES: protein motif:HMM:NF021255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HIR2850479.1"
                     /translation="MLKMDLEERIALTRAIVGLLDSWGIGAAEQIALLALPEGTRPGA
                     VRQFRQSTPFPESEQVMERVEHLIGIADALRTSYPHNAHMGNIWMNRANHRFDNRTPL
                     RAMLEDGLGGIIAVRTHLDCAYDWHISGSDAKPG"
     gene            complement(1229..>1861)
                     /locus_tag="AC16FV_10295"
     CDS             complement(1229..>1861)
                     /locus_tag="AC16FV_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HIR2850480.1"
                     /translation="ITSGKYVKGMKMHQVRLNRDVQIANAITFMAREREQVDEAYAGD
                     IIGLHNHGTIQIGDTFTEGEDIKFTGIPYFAPELFRRVVLRDPLRMKALQKGLDQLSE
                     EGATQIFRPLISNDLILGAVGALQFDVVAYRLKHEYGVECTYDNIDVATARWVDCDEP
                     KKLEEFRCNGESRLALDRAGNLTYLAPTLVNLQLTMERWPQIRFFATREH"
BASE COUNT          353 a          562 c          554 g          392 t
ORIGIN      
        1 acgccagttt taaaataaga acttaaccgc caagacgcca aggacgccaa gaacgccagg
       61 gaaagatttt gaaaatacgt tgtgccgcgg cccttagtat gcgcgacggg acttccaagt
      121 ctccctcgcg ccttcggtct cgcggcgtta ttctgcttcg caggccccgg ccgccctgcg
      181 gctgagaacc gccacaatgg ctctacgcct cgcgaagctc ggctaccggg actacgcgcc
      241 atcgcttcgc tctccctggc gcgcagcgga agagccaaaa tagaatgagt ttctcagcca
      301 cggcgccgga gtttgtagga ggtcatgtcc tgcaccagcg ccgggaaggg aacagtatcg
      361 aggccgttat attttccaat cgcctgatcc accaaggcgt aaacgttttt cacatccccc
      421 gtagcgacgg cgctatccag aagacgttgc agacccagca gaccgccata ctggattttg
      481 accagcacgc tgtggggcat gctgagcagg tcgtcctcca ccccgaatgc cggcaaggcc
      541 gcggccttca tctgctggaa caacttttcg cagcgctgat gggccacgtc ggaggtgcag
      601 gcgatgtccg gcccgccgcg acgcgtcacc tgttccgctt tttcacaccc gaactggttg
      661 gtgatcaggg tgttgctgaa ggcgcatttg taaaggcgca tttgtattcg tccatgcgtc
      721 agtcgcgtcc gcgcttatcc cggtttcgcg tccgaaccgc tgatgtgcca gtcataggcg
      781 cagtcgagat gcgtgcgcac cgcgatgatg ccaccgagcc catcctccag catcgcgcgc
      841 aacggcgtgc ggttgtcaaa acggtgattg gcgcgattca tccagatgtt gcccatgtgc
      901 gcgttgtgcg ggtaggaggt acgcagggca tcggcgatcc cgatcagatg ttcgacgcgc
      961 tccatcacct gctcgctctc cggaaacggc gtgctctgac gaaactgccg caccgcgccg
     1021 ggacgcgtgc cttccggcaa tgccagcaag gcgatctgtt cagcggcgcc aatgccccag
     1081 ctgtccagca ggccgacaat ggcgcgggtc agggcaatgc gttcttccag gtccattttc
     1141 agcatgacgt gctcagtatg gtaaatgccg ttggcgatgg ctgtaaacgc acatcatctg
     1201 taatgacatc cggccgaatg atgttgcttc agtgctcgcg cgtggcgaag aaacggattt
     1261 gcggccagcg ttccatggtg agctggaggt tgaccagggt cggggccagg taggtcaggt
     1321 tgccggcgcg gtcgagggca agccgggact cgccgttgca gcggaattcc tcgagcttct
     1381 taggttcgtc gcagtccacc cagcgcgccg tggcaacatc gatgttgtcg taggtgcatt
     1441 ccacgccata ttcgtgcttc aggcgatagg ctacgacatc gaattgcagt gcgcccaccg
     1501 ctccgaggat gaggtcgttg ctgatcagcg gccggaagat ctgcgtggca ccctcctcgc
     1561 tcagctgatc caaccccttc tgcagggcct tcatgcgcaa cgggtcgcgc agcactacgc
     1621 gccggaatag ctcgggcgcg aaatacggaa tacccgtgaa tttgatgtct tcgccttcgg
     1681 taaaagtatc cccgatctgg atcgtgccat ggttgtgcaa gccgataatg tcacccgcgt
     1741 aggcctcgtc cacctgctcg cgttcgcgcg ccatgaaggt gatggcattg gcgatctgca
     1801 cgtcgcggtt caggcgcacc tgatgcattt tcatgccttt gacgtatttg ccggaggtga
     1861 t
//
LOCUS       DBNGZE010000404         5626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1723457, whole genome shotgun sequence.
ACCESSION   DBNGZE010000404 DBNGZE010000000
VERSION     DBNGZE010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1723457"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="AC16FV_10300"
     CDS             complement(<1..950)
                     /locus_tag="AC16FV_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR2850481.1"
                     /translation="MKVVQLQAQEPVVSEIPIQPTSLDIWDKKYRLKSKTGEVIDADI
                     AGTYARVARALADVEEIPEKREEWFEKFFWALQNGAIPAGRITSNAGALQHKPATSTI
                     NCTVSRAVPDSMDGSLTSVHEAGLTLKAGCGIGYEFSTLRPKGAFVSGAGAYTSGPLS
                     FMDIFDAMCFTVSSAGGRRGAQMGTFDIAHPDIMDFIKAKREDGRLRQFNLSCLITDE
                     FMQAVKKNGSWDLVFPTNQHEYEDPDTRVTWRYWPVTEGYITNNLGQVACKIYKTLPA
                     RRLWDLIMASTYDYAEPGFILIDKINEMNNNWFCENIRAT"
     gene            complement(1244..2755)
                     /locus_tag="AC16FV_10305"
     CDS             complement(1244..2755)
                     /locus_tag="AC16FV_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR2850482.1"
                     /translation="MRDIRLVETHISWVLLTGPYAYKIKKPVNLGFLDFSTLEKRRFY
                     CAEELRLNRRLAPALYLEVVAITGSLESPVLSGTGEAIEYAVKMVQFSEETRLDRMLA
                     RGELHPQHIDLLAQELAEFHGRIAVAGNDKPFGDPEHVLEPVRQNFIQIRPRIEARDQ
                     AQLRRLEIWSEMTFAELHQTFEARKREGFIRECHGDAHLANMVWLEGRALLFDCLEFS
                     DNLRWIDVMSEVAFLVMDLDDRARPELARRALNAYLEHTGDYVGLAVFRFYQVYRALV
                     RAKVACIRLSQDGLSDTDKQQGREEYRSYAQLAERYTQPPPAALVITHGLSGSGKTWL
                     SQQLLESLDAIRVRSDIERKRLHGLAPFARSGSGIDSGIYTTQAGERTYAHLAQLAMT
                     ILRAGHTVIVDAACLKRSQRDQLRAAAEQMHAPFVILDIQAPENTLRERLRQRGTQQH
                     EASEADLAVLDRQLATREPLADDEQRQVLTIDTGALISTATLVRQLRERLAGI"
     gene            complement(2873..3805)
                     /locus_tag="AC16FV_10310"
     CDS             complement(2873..3805)
                     /locus_tag="AC16FV_10310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR2850483.1"
                     /translation="MSALICGSFAYDTIMVFQDQFKNHILPDKVHILNVSFQVPDMRR
                     EYGGCAGNIAYNLKLLGGTPLPMGTVGKDFGPYAEWMDKHGISRRHIKICEDTYTAQA
                     FITTDRDDNQITAFHPGAMNRSHETRVTDAKDAKLGIVAPDGREGMIQHARQFAEAGI
                     PFIFDPGQGMPLFDGDDLLAFLEQATWVTLNDYEAELMQERTEKSLEQLAGYVKGLIV
                     TLGARGSAVYTDGKVIEIPVVNAAAVNDPTGCGDAYRAGLLYGLSRDMDWETTGRIAA
                     LLGSIKIERHGTQNHFFTRDQFASRFRESFGYSL"
     gene            complement(3867..4589)
                     /locus_tag="AC16FV_10315"
     CDS             complement(3867..4589)
                     /locus_tag="AC16FV_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR2850484.1"
                     /translation="MFKPDKKTILLILLAIAVMGGMAYVMYATGLIDLLTSKNRLLQL
                     IRENRTHAATIFIGLQIVQVVAAPIPGEVTGFVGGILFGPVWGVVYSTIGLTIGSWIA
                     FMLARWLGRPIVERLASRETIERYDYVMKHKGLLLAFLMFLIPGFPKDILCYILGLGH
                     MQQRDFLIVSAAGRLLGTVLLTVGGTFFRDAQYGALFTVVGISLAIILFVMIYHDRVE
                     RVLQRVSGVLRKDAKDKTPPER"
     gene            complement(4688..>5626)
                     /locus_tag="AC16FV_10320"
     CDS             complement(4688..>5626)
                     /locus_tag="AC16FV_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR2850485.1"
                     /translation="GGYRQQADEKRPEDVWKGIYHEEGAFLYNEWDHRRNHYRKHWCV
                     LREIDVHPAREPFVAQTLAKYRGVLPQLRKTFEALRGENKLLKKQSNGDDVDFDALVE
                     ARADMQSGMELSERLFTKLHKFERDIAVMFMVDMSGSTKGWINNAERESLVLLCEALE
                     ILGDRYAIYGFSGMTRKRCELYRIKRFDEPYNEDVRSRIAGIQPQDYTRMGVTIRHLT
                     KLLNDIEARTKLLITLSDGKPDDYDGYRGEYGIEDTRMSLIEAKRAGIHPFCITIDET
                     ARDYLPHMYGAVNWTLVDDVRKLPLKVSDIYRRLTL"
BASE COUNT         1037 a         1637 c         1769 g         1183 t
ORIGIN      
        1 ttggtggcgc ggatgttctc gcagaaccag ttgttgttca tctcgttgat cttgtcgatc
       61 aggatgaacc cgggctcggc atagtcgtag gtcgaggcca tgatcaggtc ccacaaccgg
      121 cgcgccggca gggtcttgta gatcttgcag gccacctggc cgaggttatt ggtgatgtaa
      181 ccctcggtca ccggccaata gcgccaggtg acgcgcgtgt ccgggtcttc gtactcatgc
      241 tggttggtcg ggaataccag gtcccaagag ccgttctttt tcaccgcctg catgaactcg
      301 tcggtgatca ggcaggaaag gttgaactgg cgcagccgtc cgtcttcgcg cttggccttg
      361 atgaaatcca tgatgtccgg atgggcgatg tcgaaggtgc ccatctgcgc cccgcgccgg
      421 ccgccagcgg acgagacggt gaaacacatg gcgtcgaaaa tatccataaa agagaggggg
      481 ccggaggtat aagccccggc cccgctcaca aacgcccctt tggggcggag ggtagagaac
      541 tcgtagccga tgccgcagcc ggccttcagc gtcaggccgg cctcatgcac cgaggtcaga
      601 ctcccgtcca tggagtcggg gacggcccgg gacaccgtgc aattgatggt gctggtggca
      661 ggtttatgct ggagagcgcc ggcattggac gtgatgcgcc cggccggaat ggccccgttt
      721 tgcagggccc agaagaactt ttcaaaccat tcctcgcgct tttccgggat ttcctcgacg
      781 tcggccagcg cccgggccac acgggcatag gtgccggcga tatccgcgtc gatgacctcc
      841 ccggtcttgc ttttgaggcg gtatttttta tcccagatgt cgagagaggt gggttgtatg
      901 gggatttcgc tgacaaccgg ctcttgtgct tgtaattgga cgaccttcat tgattcctgt
      961 cctccggtgc ctgtcttatg gggcactcac tgtgccattt tttaatgaaa taccagatgt
     1021 cgggcctacg accttggcca accactatat gttgtagttt tagtctcata aggtgtctga
     1081 tgtcaagctt gcaatttttt tatgaccaag ttggagagga atcggcaagt tctaccttat
     1141 atatgttcgg acgaacttcc cgccgcgcag cgcaagtgtc ccacgtaaac cggtgacgat
     1201 cccgggagtt aaccagaaac ggcggtgaac aaccgggacc ggttcatatt ccggcgagcc
     1261 gctcgcgcaa ttgtcgcacc agcgtcgcag tggaaattaa tgcaccggta tcaatagtca
     1321 ggacctgacg ttgttcatcg tccgccagcg gttcccgggt ggccagttgc cgatccagca
     1381 ccgccaagtc agcttcggag gcctcgtgtt gttgcgtgcc gcgctggcgc aggcgctcgc
     1441 gcagggtgtt ttccggcgcc tgaatatcga gaatcacaaa aggggcgtgc atctgctccg
     1501 ccgccgcgcg cagctgatcg cgctggctgc gtttgaggca ggcggcgtcg acgatcaccg
     1561 tgtgccccgc gcgcaggatg gtcatggcga gttgcgccag gtgcgcgtac gtacgctcac
     1621 cggcttgcgt ggtgtaaatg ccgctatcga tgccggagcc gctgcgcgcg aacggggcga
     1681 gaccatgcag gcgtttgcgc tcaatgtcgc tgcgcacgcg aatggcgtcg agcgactcca
     1741 gcagctgttg cgacagccag gtcttgccgc tgccggacag accatgggtg ataacgagcg
     1801 cggccggggg aggttgcgtg tagcgctcag ccagctgcgc gtaactccgg tattcttcgc
     1861 gtccttgctg tttatccgtg tcgctcagac cgtcctgact caggcgaatg catgccacct
     1921 tggcgcgcac cagcgcgcga tagacctgat agaaacggaa tacggcaaga ccgacgtaat
     1981 ccccggtgtg ttcgaggtaa gcgttcagcg cgcgccgcgc cagctcggga cgagcacgat
     2041 catcgaggtc catgacgaga aaggccactt cgctcatgac atcgatccag cgcaggttgt
     2101 cgctgaattc gaggcaatcg aagagcaggg cgcgcccctc gagccacacc atgttggcaa
     2161 gatgcgcatc gccatggcac tcgcggatga agccttcgcg tttgcgggcc tcgaatgtct
     2221 ggtgcagttc ggcgaaggtc atttcgctcc agatttccag gcgacgcaat tgtgcctggt
     2281 ccctggcttc aatacgtggg cggatctgta tgaaattttg tcgtaccggt tccagcacat
     2341 gttccggatc gccgaagggt ttgtcgttgc cggccacggc gatgcggccg tggaactcgg
     2401 ccagttcttg cgccagcaga tcgatgtgtt gtggatgcag ctcgccgcgt gccagcatgc
     2461 ggtcgagcct tgtctcctcg gagaactgga ccatcttgac cgcgtattcg atggcctcgc
     2521 cggtgccgga gagcacgggc gattcgagcg agccggtgat ggcaacaact tccagataaa
     2581 gcgctggcgc gaggcgccgg ttcaggcgca attcttccgc gcaatagaac cggcgttttt
     2641 ccagagtgct gaaatcaagg aaaccgagat tgactggctt cttgatcttg taggcgtagg
     2701 ggccggtcag cagcacccag gaaatatggg tctcaaccag ccggatatcc cgcaccggat
     2761 ggccgtaaca ggcagggtgg tgcagcgcct ggatcagggg tggcagtcgg gtgtccgcca
     2821 gggccgtccc tccttgtgtc gacaggtccg gccgggtatc gccggaaaac cgctacagac
     2881 tatacccgaa actctccctg aagcgggacg cgaactggtc gcgggtaaag aagtggttct
     2941 gggtgccgtg acgctcgatc ttgatggacc ccaggagcgc ggcgatgcgg ccggtggttt
     3001 cccagtccat gtcgcgcgac aggccgtaga gcagtccggc gcgataggcg tcgccgcaac
     3061 cggtcgggtc gttgaccgcg gcggcgttga cgaccgggat ttcgatcacc ttcccgtcgg
     3121 tgtagacggc ggagccgcgc gcgccgagcg tgacgatcag ccccttcacg tatcccgcca
     3181 gttgctcgag cgatttttcg gtgcgttcct gcatcagctc ggcctcgtag tcgttgagcg
     3241 tgacccaggt ggcctgttcc aggaacgcga gcaggtcgtc gccgtcgaac agcggcatgc
     3301 cctgaccggg atcgaagatg aacggaatgc cggcttcggc gaactggcgc gcgtgttgga
     3361 tcatgccctc gcgaccatca ggcgcgacga taccgagttt ggcgtctttg gcatccgtca
     3421 cgcgggtttc gtgcgaacgg ttcatggcgc cgggatgaaa cgcggtaatc tggttgtcgt
     3481 ccctgtcggt ggtgatgaaa gcctgcgcgg tgtaggtgtc ctcgcatatc ttgatatggc
     3541 gacgactgat gccgtgcttg tccatccact cggcataagg gccgaagtcc ttgccgaccg
     3601 tgcccatggg caggggtgtg ccaccgagca gtttgaggtt gtaggcgatg ttgccggcgc
     3661 agccgccgta ttcgcgccgc atatccggta cctggaacga tacgttcagg atatgcacct
     3721 tgtcgggcag gatgtgattc ttgaactggt cctggaacac catgatggtg tcgtaggcga
     3781 aggaaccgca gatcagcgct gacatgacga accccgggaa ttgatgcgaa tgctgcgcga
     3841 gtatacccgc tcttttggcg tcatggttat cgctcgggcg gcgtcttgtc ttttgcatcc
     3901 ttgcgcaaga cccccgagac gcgttgcagg acgcgttcga cccggtcgtg gtagatcatc
     3961 acgaacagga tgatggccag acttatcccg acgacggtga aaagcgcgcc atactgggcg
     4021 tcgcggaaga aggtcccgcc cacggtgagc agtacggtgc cgagcagccg tccggccgcc
     4081 gagacgatca ggaagtcgcg ctgctgcatg tggccgagac ccaggatgta gcacaggatg
     4141 tccttgggga aaccgggaat gagaaacatc aggaacgcga gcagcaggcc cttgtgcttc
     4201 atcacgtagt cgtaacgttc gatggtctcg cggctggcga gccgttccac gatcggccgc
     4261 cccagccacc gcgccagcat gaaggcgatc cacgagccga tggtcaaacc gatggtggaa
     4321 tacaccactc cccacaccgg accgaacaga ataccgccca cgaaaccggt aacctcgccg
     4381 gggatcggcg cggccacgac ctgcacgatc tggaggccga tgaaaatcgt cgcggcgtgc
     4441 gtgcgatttt ccctgatcaa ctggagcagc cggttcttgc ttgtgagcag atcgatcaac
     4501 ccggtggcgt acatgacata agccatgccg cccatgaccg cgattgccag caagatcagc
     4561 aggatcgtct tcttgtccgg tttgaacatg tcggtggcgt ggccctgttt atttcattta
     4621 tatgggctgg atggctgcag gataaagcca tcgtaccatt cacggtcttt tgtccccaag
     4681 gcgggaatta cagcgtcagc cgccggtaaa tatccgaaac cttcagcggc agttttcgga
     4741 cgtcgtcgac cagcgtccag ttcaccgcgc cgtacatgtg cggcaggtaa tcgcgcgcgg
     4801 tctcgtcgat ggtgatgcag aacggatgaa tgccggcgcg cttggcctcg atcaggctca
     4861 tgcgcgtgtc ctcgatgccg tactcgccgc ggtagccgtc gtagtcgtcg ggtttgccgt
     4921 ccgagagcgt gatcagcagc ttggtgcgcg cctcgatgtc gttcaacagt ttggtgagat
     4981 gacggatggt cacgcccatg cgcgtgtaat cctgcggctg gatgccggcg atgcggctcc
     5041 gcacgtcttc gttgtaaggc tcgtcgaaac gcttgatgcg atacagttcg cagcgcttgc
     5101 gcgtcatgcc ggagaagccg tagatggcgt aacggtcgcc gaggatttcc agcgcttcgc
     5161 acagcaacac cagcgattcg cgctcggcgt tgttgatcca gcccttggtc gagccgctca
     5221 tgtccaccat gaacatcacg gcgatgtcgc gctcgaactt gtgcagcttg gtgaacagcc
     5281 gttccgacag ctccatgccg ctttgcatgt cggcccgggc ctccaccagc gcatcgaaat
     5341 cgacgtcgtc accgttgctc tgtttcttga gcagcttgtt ctcgccgcgc agcgcctcga
     5401 aggtcttgcg cagctgtggc aacaccccgc ggtatttggc gagtgtctgc gccacgaaag
     5461 gttcgcgcgc aggatggacg tcgatctcgc gcagcacgca ccagtgcttg cggtaatgat
     5521 tgcgccggtg atcccactcg ttgtacagaa acgcgccttc ctcgtgatag atgcctttcc
     5581 acacgtcctc cggccgtttc tcgtccgctt gttggcgata accacc
//
LOCUS       DBNGZE010000405         4449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_473017, whole genome shotgun sequence.
ACCESSION   DBNGZE010000405 DBNGZE010000000
VERSION     DBNGZE010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_473017"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="AC16FV_10325"
     CDS             <1..904
                     /locus_tag="AC16FV_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR2850486.1"
                     /translation="MGNIHTDEVDPIAEAEVYLAYGRDETAEEILKEAMVKNPDRQEI
                     KLKLLEIYHQRNDVSAFETLAEELYAAQGGRGGKVWDKVEEMGRKLNPDNPMFRGGAP
                     GKAPAGAAPARPAAVAKTAPATTTATTPAAVDFQASATETAKTELASEFDFDIDTAAA
                     GEAKSAAGGDDFSVMMPETPSKAKEEADLEALDLGAAADSGLDFDIGGGEAQAAPAGN
                     EIKWEPAAPAAAAEVDIEAAAAPAGEGGSSAQWDEAATKLDLAKAYIDMGDAEGARSI
                     LQEVMAEGSETQKKQAQELSSQIA"
     gene            complement(1049..1732)
                     /locus_tag="AC16FV_10330"
     CDS             complement(1049..1732)
                     /locus_tag="AC16FV_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850487.1"
                     /translation="MKRLAALLLIFILPALVEAAGPLVPADNYNSTLDFSLRFARTKM
                     PLDYGNRTSDTTSHWLGVSLREKASKRVTLGLYGGYAWLTQTNNPVTAGIELGGYHAG
                     FSLHGVLLEGKRASLYGALDYSYQKMDHKSDTQTVVIDWVQPQAQLGAMLTLSRNWRL
                     YGGGSYGKIDGEERATGSVNHTLSFERGAQSGGFLGLDLNVETDGYIGVEARSGLTRG
                     VDIYFKRKF"
     gene            complement(1729..2235)
                     /locus_tag="AC16FV_10335"
     CDS             complement(1729..2235)
                     /locus_tag="AC16FV_10335"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR2850488.1"
                     /translation="MSRARRVSLIAAMAENRVIGINNTLPWRLPADLRHFRQLTTGHH
                     VIMGRRNYESIGKPLPDRTNIVVTRNPAYHAPGCQVKQSLVEALQDIQDDPEIFIIGG
                     AEIYRQSIGDADRIYLTLVHANIKGDTWFPEFDAREWNEVSRARHEADEKNPYAYSFV
                     TYDRKNGE"
     gene            complement(2241..3035)
                     /locus_tag="AC16FV_10340"
     CDS             complement(2241..3035)
                     /locus_tag="AC16FV_10340"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR2850489.1"
                     /translation="MRQYLDLMRHVMQHGVRKDDRTGTGTLSVFGHQMRFDLAQGFPL
                     LTTKKVHTRSIIHELLWFLRGDTNIRYLKEHGVSIWDEWANESGDLGPVYGAQWRSWP
                     TPDGRQIDQISQVVRDIRQNPDSRRLIVSAWNVAEIERMALPPCHLLFQFYVANGRLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHLYLNHLEQVKT
                     QLVREPRPLPSMRLNPEKTSLFDFTFDDFELLNYDPHPSIKAPVAV"
     gene            complement(3115..3810)
                     /locus_tag="AC16FV_10345"
     CDS             complement(3115..3810)
                     /locus_tag="AC16FV_10345"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR2850490.1"
                     /translation="MIPLHDDNPTTIKPLVTVTLIVMTSLIFLWQVSLGARGFEAAVL
                     SLGVIPATLLGGQSLPPELALVPPGITVFTSMFLHGSWMHLIGNMLYLWIFGNNVEDA
                     MGHVRFVIFYLLCGIAAVFAQALPNVESTIPMIGASGAISGVLGAYLLLYPRAHVLVA
                     IPLGFYMRTMYLPAMVVLGFWFVLQLLNSVLADSGKGGVAFGAHIGGFVAGMVLLPLF
                     KHRHVRLFAPPRH"
     gene            complement(3814..>4449)
                     /locus_tag="AC16FV_10350"
     CDS             complement(3814..>4449)
                     /locus_tag="AC16FV_10350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR2850491.1"
                     /translation="LSAQLLRAKSFEGASGFKTNETMRARIALHAAIPVLNLGLDYYA
                     DWSSIVIYPGDFRVHDEYMDEHGVVHREIMDLCGQSLSQGPIVLSWEAMREEDETPAD
                     HDLVIHECAHKLDLLNGDANGFPPLHPGMDARAWANDFHAAYRELCTDPGMRDASRID
                     PYASEDPAEFFAVMSETFFTAPHIVYEDHPTVYKQLQRFYRQDPHQVMQQS"
BASE COUNT          960 a         1234 c         1392 g          863 t
ORIGIN      
        1 catgggcaac atccacaccg acgaagtgga cccgattgcc gaggccgagg tctatctcgc
       61 ctacgggcgc gacgaaacgg ccgaggagat tctcaaggag gcgatggtca agaatcccga
      121 tcgccaggag atcaagctca agctgctcga gatttatcat cagcgcaatg atgtctccgc
      181 gttcgagacg ctggcggaag aactctatgc cgcgcagggc ggacgcggcg gcaaggtctg
      241 ggacaaggtc gaagagatgg ggcgcaagct caatccggac aatccgatgt tccgcggcgg
      301 cgctccgggc aaggccccgg caggcgctgc tcccgccagg cccgcggctg tcgccaagac
      361 cgcgcctgcc acgacaactg caacgacacc cgctgcggtt gactttcagg cctctgccac
      421 cgaaacggcg aagacggaat tggccagcga attcgatttt gatatcgaca cagccgcggc
      481 tggggaagcc aagtctgctg ccggcggcga tgacttctcg gtgatgatgc cggagacgcc
      541 atccaaagcc aaggaagaag ccgatctcga ggcgctcgat ctgggcgccg cggcggacag
      601 tggattagat ttcgatatcg gtggcggtga agcgcaggcc gcgcctgccg gcaatgagat
      661 caagtgggaa ccggcggcac cggccgccgc ggcggaggtg gatatcgaag cagccgccgc
      721 acccgcgggt gaaggcggat cctcggcgca gtgggatgaa gccgcgacga aactggatct
      781 ggccaaggcc tatatcgata tgggcgatgc cgaaggcgcg cgcagcattc tgcaggaagt
      841 gatggcggaa ggcagcgaga cacagaagaa gcaggcgcag gaactatcct cacagattgc
      901 ttaagtctct aacgaaataa atagacagga ttaacaggat ttctcaggat tcacaggata
      961 aaagatttta gaatcaaaaa ttcctgttaa tcttgtttta atcctgtaaa tcctgtgaat
     1021 tgttgttttc tgatctttcg aagtccgttc agaattttcg tttgaaataa atatccacgc
     1081 cgcgtgtcag gcccgagcgg gcctcgacgc cgatatagcc gtccgtttcc acgttcagat
     1141 cgagccccag aaatcctcct gactgcgcgc cacgttcgaa cgagagcgtg tgattgacgg
     1201 aaccggtggc acgttcctcg ccgtcgatct tgccgtaact gccgccaccg tagagccgcc
     1261 aattccgtga cagcgtcagc atggcgccga gctgcgcctg tggttgcacc caatcgatca
     1321 cgaccgtctg ggtatcgctc ttatggtcca tcttctggta gctgtaatcg agcgcgccat
     1381 acagagaggc gcgctttcct tccagcagaa ctccatgcag tgagaatccc gcatgatagc
     1441 cacccagttc gattccggct gtcacggggt tgttggtctg ggtcagccag gcgtatccgc
     1501 cgtacagacc cagcgtcacg cgtttactgg ccttctcgcg caagctgacg cccagccagt
     1561 ggctggtggt gtcgctggtg cggttgccgt agtcgagcgg cattttggtg cgggcaaatc
     1621 gcagtgaaaa atccagggtt gaattgtagt tatccgccgg caccagcgga ccggcagcct
     1681 cgaccagcgc tggcaggatg aaaatcaaca acaaggcagc caggcgtttc attccccgtt
     1741 tttcctgtcg tatgttacga agctgtatgc gtagggattt ttttcatccg cctcatggcg
     1801 tgcgcggctg acctcgttcc attcacgcgc atcgaactcc ggaaaccagg tgtcgccctt
     1861 gatattggca tgcaccagcg tgaggtaaat acgatcggca tcaccgatgg actggcgata
     1921 aatttccgcg ccgccgatga tgaaaatctc cggatcgtcc tgaatgtcct gaagcgcctc
     1981 cacgagcgac tgttttacct ggcagcccgg tgcgtggtag gcggggttgc gcgtaaccac
     2041 gatgttggtg cgatcgggca ggggcttgcc gatggattcg taattgcgac gccccatgat
     2101 cacatgatgg ccggtggtga gctgccggaa gtgacgtaga tcggccggca gtcgccacgg
     2161 gagcgtgtta ttgatgccga tcacgcggtt ctccgccatg gcggcgataa gcgaaacgcg
     2221 ccttgcccgg gacacggcgt ttataccgcg accggcgcct tgatggaggg atggggatcg
     2281 tagttcagca gttcgaaatc gtcgaaagtg aaatcgaata acgaagtctt ctcgggattg
     2341 aggcgcatgg atggcagcgg acgcggctcg cgcacgagtt gtgttttaac ctgttcgaga
     2401 tggttcaaat aaagatgggc gtcgccgagt gtgtgtatga attccccggg cttgagaccg
     2461 gtgacctgcg cgatcatcat ggtcagcagg gcgtaagagg cgatattgaa cggcacgccg
     2521 aggaaaatat cggcgctgcg ctggtacagc tgacaggaga gccgcccgtt cgcaacatag
     2581 aactggaaca agagatggca gggcggtagc gccatgcgtt cgatctcggc cacgttccag
     2641 gcgctgacaa tcaaccggcg tgaatccggg ttctggcgga tgtcccgcac tacctggctg
     2701 atctggtcaa tctgacgacc atcgggcgtg ggccaggaac gccattgcgc gccgtaaacc
     2761 ggtccgagat cgccgctttc gttagcccat tcgtcccaga tgctgacgcc gtgttccttc
     2821 aggtagcgga tgttagtgtc gccgcgcagg aaccacagca attcatggat gatggacctg
     2881 gtgtgaacct tcttggtggt gaggaggggg aaaccttgcg ccagatcgaa gcgcatctgg
     2941 tgaccaaaca cggagagcgt tccggtgccg gtgcgatcgt ctttgcgcac cccgtgttgc
     3001 attacatggc gcatcaggtc gaggtactgc cgcatgcctt tgtctccgtt gctacgatgt
     3061 tacaggacgc cagacgggtc gtagtatatc cgctcgccgt atcggacgca gccgttaatg
     3121 ccgtggcggc gcgaacaggc gcacgtgccg gtgcttgaac agcggcagca gcaccatgcc
     3181 cgcgacgaaa ccaccgatat gcgcgccgaa agccacgccg cccttgccgg agtcggcaag
     3241 caccgaattt aaaagttgca gcacgaacca gaaaccgagc acgaccatgg ctggcaggta
     3301 catggtacgc atgtaaaagc ccaacggaat cgcgaccaat acatgggcgc gcggatagag
     3361 cagaagatag gccccgagga cgccagaaat cgcgccgctg gcgccgatca tgggtatcgt
     3421 ggactcgacg ttcggcaggg cctgcgcgaa caccgcggcg atgccgcaca gcagatagaa
     3481 gatcacgaag cgcacatgac ccatggcgtc ctcgacgttg ttgccgaaga tccagagata
     3541 cagcatgttg ccgatcaggt gcatccagct gccatgcagg aacatggagg tgaacaccgt
     3601 gatgcccggc ggcaccagcg cgagttctgg tgggagcgac tggccgccca gcagggtggc
     3661 cgggatgacg cccaggctca ggacggctgc ttcgaaaccc cgggcgccca gggaaacctg
     3721 ccatagaaaa atcagggagg tcatgacgat aagggtcacc gtaacgagcg gcttgatcgt
     3781 cgtgggattg tcgtcgtgca gcgggatcat gaatcagctt tgttgcatga cctgatgcgg
     3841 gtcctggcga tagaaacgtt gtagctgctt atacaccgtc ggatgatctt cgtaaacgat
     3901 gtgtggcgca gtaaaaaagg tttcgctcat cacggcaaaa aattccgccg ggtcctcgga
     3961 ggcgtaagga tcaatgcggc tggcgtcgcg cattcccgga tcggtgcata gttcccggta
     4021 ggcggcgtgg aaatcgttcg cccaggcgcg cgcgtccatg cctggatgca acggcgggaa
     4081 tccattggca tccccattga gcaggtccag cttgtgcgcg cattcgtgga tcacgagatc
     4141 atgatcggcg ggcgtctcgt cttcttcgcg catggcctcc caggaaagca cgattggacc
     4201 ctgggacagg gactgaccgc aaaggtccat gatttcgcgg tgcacgacgc cgtgctcgtc
     4261 catgtattcg tcgtgcacgc ggaaatcgcc cggatagata acgatggaac tccagtcggc
     4321 gtaataatcg agcccgagat tgagcacggg aatggcggca tgcaacgcga tgcgcgcgcg
     4381 catcgtttcg ttggttttga atcccgatgc gccttcaaag gacttggcgc gcagaagctg
     4441 tgccgagag
//
LOCUS       DBNGZE010000406        11154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_194999, whole genome shotgun sequence.
ACCESSION   DBNGZE010000406 DBNGZE010000000
VERSION     DBNGZE010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_194999"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16FV_10355"
     CDS             <1..291
                     /locus_tag="AC16FV_10355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase family protein"
                     /protein_id="HIR2850492.1"
                     /translation="DTGVECPECKQATMLKRKSRYGKVFYSCARYPDCKYAVWNPPVK
                     EPCPQCGWPILTIKTTKRRGTEKVCPVKECGFAEPYEGEVPFAEPPKVAAGV"
     gene            complement(316..1062)
                     /locus_tag="AC16FV_10360"
     CDS             complement(316..1062)
                     /locus_tag="AC16FV_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2850493.1"
                     /translation="MESLDSSLFPWLLAFIAVAATLYSSVGHGGASGYLAAMALFGVS
                     AALMKPAALAMNIVVAGLVFARLWRAGFFNARLFWPFALGSIPLAFLGGAIQLHERAY
                     QYLVAVALLVAAWRLLLTSHEPPTREAPHTGIALPMGAGLGFVSGLTGVGGGIFLSPL
                     LLFLRWANMRTTAAVSAAFILVNSIAGLVGLLSTGVMLSRGLPWMMLAAVGGAIVGSE
                     LAVRRLAPVRLRQLLGVVLVIAAVKMFLTA"
     gene            1125..2282
                     /gene="mltA"
                     /locus_tag="AC16FV_10365"
     CDS             1125..2282
                     /gene="mltA"
                     /locus_tag="AC16FV_10365"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR2850494.1"
                     /translation="MHLILAFRVKETISKLVVAGALCLLVSCVSAPRAPGIGQPVPWT
                     ELPGWQDDRLAQAWPALQNSCQKLPARDARWSDLCADAALFPAPDDDTARAFFETRFV
                     AYEMSANNGAADGLITGYYEPLLHGSLAKTKRFRYPVYGRPDDLVVVDLGGLYPELQG
                     KRYRGRLNSNRVVPYYSRAEISNGKRPPDDAVLAWVDDPVALFFLEIQGSGRIRLPDG
                     KMLPVGYADQNGHPYIAIGRTLVESGVMKLEEATMPGIRAWLKANPGKAQAVLNSNPS
                     YVFFNLREPDSSGPVGALNVPLLPERSIAVDPVFIPLGSPVWFDTSLPDNNSARPYRR
                     LVFAQDTGGAIRGPVRADLFLGFGETAERIAGAMRQRGKLYVLLPADRELN"
     gene            complement(2289..2795)
                     /locus_tag="AC16FV_10370"
     CDS             complement(2289..2795)
                     /locus_tag="AC16FV_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850495.1"
                     /translation="MASFRLEQPATLAQLWESLARQYPEGVMFNGYVRLAPLRLIAIA
                     RPAIDGRAVLKNAAQYYTRPMESAPEAWTYVVGIATAKSTTSRMGRAWLSSLLTARPD
                     SAGTGTGLAHALWLKSAPADVQAPPTRENVNAVGQLVVDQTTLMEGELRLYPVTHAGG
                     CNDAARAR"
     gene            complement(3298..3642)
                     /locus_tag="AC16FV_10375"
     CDS             complement(3298..3642)
                     /locus_tag="AC16FV_10375"
                     /inference="COORDINATES: protein motif:HMM:NF020345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HIR2850496.1"
                     /translation="MSDNAAVEYPELLADLADKLTEALVVRGLEREKAKEIGREAAEF
                     IRTEWGGQLVYIPKGTLFALTQRDLEIWREFNGHNHADLARKNNVTLQRIYQIVAAMR
                     EQEVRRRQGKLF"
     gene            complement(3635..4177)
                     /locus_tag="AC16FV_10380"
     CDS             complement(3635..4177)
                     /locus_tag="AC16FV_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850497.1"
                     /translation="MQITCPCCFARYAIEAALTDDAARQAVAAALKLPAPLGDLMLRY
                     MAFFRPSKRALSWDRAAKLLNELIDPIQAARVERHGRAWPAPLHYWREALEQMIQRRD
                     KLQLPLKSHGYLFEIVSGIASKTEGQAEAAVEQQKRHPRRAATSQPAPIGKIMDPSRV
                     EQHLEQLKGATKPGKGEVNE"
     gene            complement(4177..4452)
                     /locus_tag="AC16FV_10385"
     CDS             complement(4177..4452)
                     /locus_tag="AC16FV_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850498.1"
                     /translation="MFDCCMNHVRLWEETQGGKLPASEHAPSCPNYQTKLYKRVRLNE
                     CNSASFIDTQKGAAAFIAESDEPSEYIVEDVYLTEDQFEKLPDFEGF"
     gene            complement(4454..4738)
                     /locus_tag="AC16FV_10390"
     CDS             complement(4454..4738)
                     /locus_tag="AC16FV_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850499.1"
                     /translation="MDCRHIKLENGTTAIVCGRGRRAERCRWCAHAGAFQCDWKLGNG
                     KTCDKHLCAEHALEVAPDKHLCPEHQKTYQQWLARARRLNLMVAFNLRRE"
     gene            complement(4738..4995)
                     /locus_tag="AC16FV_10395"
     CDS             complement(4738..4995)
                     /locus_tag="AC16FV_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850500.1"
                     /translation="MKNRIEDLRDHLFETIESLKSDKNPMEIDRAKAIADVAKVMIES
                     AKVEVEHLKVLEGLGMRVQANPDGSIGTGFIATAPRLKAIK"
     gene            complement(4967..5644)
                     /locus_tag="AC16FV_10400"
     CDS             complement(4967..5644)
                     /locus_tag="AC16FV_10400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR2850501.1"
                     /translation="MTIKPWTEPELRRLRHFYPHIKTEHLARVLRRPLDRVYSKAQVL
                     KLCKTPEYLASPDACRLRRDMLSTRSVACRFQKGHAPANKGLRRPGWYAGRMRETQFK
                     KGQVSRKWKPIGSERLVDGYRYTKISDIRCVPWTRNWKPTHILLWEKHYGPVPRGYAV
                     KFIDGDRDRIVLDNLCLVSRADLARLNVMWNRYPRELCEVIQLRGALKRQINRRLRNE
                     EPHRRSA"
     gene            complement(5641..6105)
                     /locus_tag="AC16FV_10405"
     CDS             complement(5641..6105)
                     /locus_tag="AC16FV_10405"
                     /inference="COORDINATES: protein motif:HMM:NF018011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HIR2850502.1"
                     /translation="MEIGEMNTANDTRRRELAKIHLAAKALGLDAADKDPNSDYRSML
                     WAVARVRSAADLDEAGRRRVRDHLKACGFQTARGRAHPGKPHNLASGERGPQLKKIEA
                     LLAEAARPWAYADGMAKRMYHVERVAFCNPAQLQGIIAALVKDAQRHNRRTQ"
     gene            complement(6268..6546)
                     /locus_tag="AC16FV_10410"
     CDS             complement(6268..6546)
                     /locus_tag="AC16FV_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850503.1"
                     /translation="MNGLVLHDEIMKFTAELEKAPRWALAYALASSSIYSKLPFAPDA
                     ETAIAAARLEILPHVEEACKTARIVAYGHRTERLIQKINQVLEDVRGS"
     gene            complement(6543..6962)
                     /locus_tag="AC16FV_10415"
     CDS             complement(6543..6962)
                     /locus_tag="AC16FV_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850504.1"
                     /translation="MALATCTQVTSSADLSVQVCGSCGGIYAIALRYRQEKIEQGGYW
                     NCPYCKCSWGYGTSQIDRLKAQLAEKEREVEQERKRKEWAQQEARITERRRRALKGQL
                     TKTKKRIGHGVCPCCQRSFENLRRHMTTKHPTYTKIA"
     gene            complement(6986..7192)
                     /locus_tag="AC16FV_10420"
     CDS             complement(6986..7192)
                     /locus_tag="AC16FV_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850505.1"
                     /translation="MAEYEVKFLVRGIVTVNVQANSLEQAIATARHRHNRKFFLKSKG
                     TEVLEENSEICGVDNLELWGKTSD"
     gene            complement(7355..7585)
                     /locus_tag="AC16FV_10425"
     CDS             complement(7355..7585)
                     /locus_tag="AC16FV_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850506.1"
                     /translation="MKRHEQKLHALIENLDRCLIAFHDGDAAALVAALEDHIVRSFEL
                     RDLVRAEGGPTPPETQERTGHHPLSIVRRSVR"
     gene            complement(7582..7956)
                     /locus_tag="AC16FV_10430"
     CDS             complement(7582..7956)
                     /locus_tag="AC16FV_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850507.1"
                     /translation="MPRPDRNPWTRTQLARLREMRADGCSFEEIAAEVNHTAQSCHSK
                     AYTLGIHSPVRRKRRVDARMPAKSAYTGRVTRDSRAKSTLRPCLCCRQDFPSQGAHNR
                     LCPSCRRQNDDTVFTTPAVVLR"
     gene            complement(7943..8467)
                     /locus_tag="AC16FV_10435"
     CDS             complement(7943..8467)
                     /locus_tag="AC16FV_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HIR2850508.1"
                     /translation="MTDLKDIETLARAYADEYQQLAADVEALESAIRALKKKALPAIK
                     RGAERAAVAKEKLKAAIEAAPQLFEKPRTRLFHGVKVGLQKGKGETQIPDEEKTIALI
                     RKHLEDQAEVLIKTEETLVKKALANLAAADLKRIGVNVIEAGDQVVVKVADSDIEKLV
                     DALFKDEEVKRAAA"
     gene            complement(8464..8763)
                     /locus_tag="AC16FV_10440"
     CDS             complement(8464..8763)
                     /locus_tag="AC16FV_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850509.1"
                     /translation="MTTLKNCKPRTRASWSRRCVRAWLRSGGKIVRLLIVSDYRPCEH
                     GGEPREYIKRITHRRWPTGIHLARGEYSPSYAAWYAALPADQWCEIVAIPLRSAA"
     gene            complement(8773..8904)
                     /locus_tag="AC16FV_10445"
     CDS             complement(8773..8904)
                     /locus_tag="AC16FV_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850510.1"
                     /translation="MESLQRHPADAIYGIQNAHVLKGIAAMKRISLPDLRGQLHLPT"
     gene            complement(8913..9137)
                     /locus_tag="AC16FV_10450"
     CDS             complement(8913..9137)
                     /locus_tag="AC16FV_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850511.1"
                     /translation="MSPRGWRVTAGFFAVFAGILTISCLHAEWRSHTESRRLLAPTET
                     LTLVETVVDGMRVTCVQREDHIHGTKSLSC"
     gene            complement(9134..9520)
                     /locus_tag="AC16FV_10455"
     CDS             complement(9134..9520)
                     /locus_tag="AC16FV_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR2850512.1"
                     /translation="MNSANVRAATSSWDDDLPDWVQVLAEQCDLRSQRIVADEIGYSS
                     AVINQVLRHRYTGSLKKVEAAVRGAYLDAKVNCPVLGEIGLHRCLQHQRAKFQATNST
                     RVRLYKACRGVGGPRCKHSKINGGAK"
     gene            complement(9517..10110)
                     /locus_tag="AC16FV_10460"
     CDS             complement(9517..10110)
                     /locus_tag="AC16FV_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850513.1"
                     /translation="MSLQPARLKSTTSSPAWRDRIWAFVRKHKQVTIDDVLTTGLPRR
                     LVSDYLNGLERAGFIKQETTGELYHLVRDVGPEAPVLNHRGQAPAGFCVRDALWRTMK
                     LLKEFTVIDLAVNASTEQCRVTERDARVYLRALVAAGYVAALARDAEGRPPGGRLLRY
                     RFLNSRNTGPRPPLLQQVLQVVDLNEGKVVWQAGGDA"
     gene            complement(10107..10841)
                     /locus_tag="AC16FV_10465"
     CDS             complement(10107..10841)
                     /locus_tag="AC16FV_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2850514.1"
                     /translation="MSKKEQSVNTTAPLANVAICMRAMEIAMKRQSHLPGIVAFYGHS
                     GWGKSASSAYAANHYRAYYVEVKSSWHKKALLEGILLEMGVSPRGTVPELTNLVAEHL
                     VLSGRPLILDEFDAVVNHNLVELVRDIYESSNAPILIIGEERLEVNLRRYERFHNRVL
                     DWVPAQPASMADAKHLRTLYCQRAKVADDLLARIHEVTRANVRRICINLERAQELASQ
                     EHWKEIDLAKWGNREFYTGEAPQRHA"
     gene            complement(10910..>11154)
                     /locus_tag="AC16FV_10470"
     CDS             complement(10910..>11154)
                     /locus_tag="AC16FV_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850515.1"
                     /translation="EEHAIEEQLVAARTQPQVIELNDTEESRYARARSLERRLHEGLP
                     VDGRDREWLASYQTCAEYRAQKNFHESFGLEAGEAG"
BASE COUNT         1922 a         3455 c         3534 g         2243 t
ORIGIN      
        1 gacaccggtg tggaatgtcc ggaatgcaaa caggcgacca tgctcaagcg caagtcgcgc
       61 tacggcaagg tgttctattc ctgcgcgcgc tatccggact gcaagtacgc ggtatggaat
      121 ccgccggtca aggagccgtg cccgcaatgc ggctggccga tcctgaccat caagaccacc
      181 aagcgccgtg gcaccgagaa ggtctgcccg gtcaaggaat gcggcttcgc cgagccgtac
      241 gagggcgaag tgccgttcgc ggagccaccg aaggtcgccg ccggggtctg atgcaatcaa
      301 gccgcagcgg cttgatcagg cagtgaggaa catttttacc gccgcgatga ccaacacgac
      361 acccagaagc tgacgtaatc gcaccggcgc gagccggcgc accgccagtt ccgatcccac
      421 gatcgcgccc ccaaccgccg ccagcatcat ccatggcagg ccgcgcgaca gcatgacacc
      481 ggtgctgagc aggcccacca gaccggcgat ggaattgacc aggataaaag ccgccgagac
      541 ggccgccgtg gtacgcatgt tggcccagcg caggaacagc aacagcgggc tcaggaagat
      601 cccgccgccg acaccggtca ggccggaaac aaacccgagc ccggcgccca tcggcagcgc
      661 tatgcccgta tgtggcgctt cgcgcgtggg cggctcgtga ctggtcagga gcagccgcca
      721 cgcggccacg agcagggcga ccgcgacgag atactgatac gcgcgttcgt gcagttgtat
      781 ggcaccgccg agaaacgcga gcgggatcga accgagcgcg aacggccaga ataaccgggc
      841 gttgaaaaat ccggcgcgcc acaggcgcgc gaacaccagc cctgccacca cgatattcat
      901 ggcgagcgca gccggtttca tgagagcggc cgaaacgccg aacagcgcca tcgccgcgag
      961 atagccagaa gcgccgccat gaccgacgga agagtagagc gtggccgcca ccgcgataaa
     1021 ggcgagcaac cacgggaaca aggacgagtc gagcgattcc aaaattatgt gcttggtggc
     1081 gcgagacggt atgacgtgct agaaaatcag gcgatatttg aaacatgcat cttatcttgg
     1141 cttttcgtgt gaaagaaacg atcagcaaac tggttgtcgc tggcgcgctt tgtctgctgg
     1201 tctcctgcgt cagcgcgccg cgcgcgcccg gcatcgggca gccggttccc tggacggaat
     1261 tgccgggctg gcaggacgac cgtctggcgc aggcgtggcc ggcgttgcag aacagttgcc
     1321 agaaattgcc cgcgcgcgac gcgcgctgga gcgatctgtg cgccgatgcc gcgctgtttc
     1381 ccgccccgga cgatgacacc gcgcgcgcct ttttcgagac gcgctttgtc gcgtacgaga
     1441 tgtccgccaa caacggcgcc gccgatggac tgatcaccgg ttattacgaa ccgctgctgc
     1501 acgggagcct ggccaagacc aagcgcttcc gctacccggt ctacggacga ccggatgatc
     1561 tggtggtggt ggacctcggc gggctttatc cggaactgca gggcaagcgc taccgcggac
     1621 gactgaacag caatcgcgtg gtgccctact attcgcgcgc cgagatcagc aacggcaaac
     1681 gcccgcccga cgatgccgtg cttgcctggg tcgacgatcc ggtggcgctg ttctttctgg
     1741 aaatccaggg ctccggccgc atccgcttgc cggacgggaa gatgctgcct gtcggctatg
     1801 ccgatcagaa cggccatccc tatatcgcca tcgggcgcac gctggtcgag tcgggcgtca
     1861 tgaagctgga ggaggcgacc atgccgggca tccgcgcctg gctcaaggcc aatccgggca
     1921 aggcgcaggc ggtgttgaac agcaacccga gttatgtctt tttcaatctg cgcgaaccgg
     1981 acagctcggg tccggtgggc gcgctcaacg tgccactgct cccggagcgt tccatcgcgg
     2041 tggatcccgt tttcattccg ctgggaagtc cggtgtggtt cgatacttcc ttgccggaca
     2101 ataacagtgc gcgtccgtat cggcggctgg tattcgccca ggataccggc ggcgcgattc
     2161 gcggtccggt gcgcgccgat ctttttctcg gtttcggcga gaccgccgag cgcatcgccg
     2221 gcgcgatgcg tcaacgcggt aaactctatg tgctgctgcc tgccgatcga gagttaaact
     2281 gaacgatttc agcgggcgcg cgccgcgtcg ttgcaaccgc cggcatgggt cacgggataa
     2341 agcctgagtt ccccttccat cagcgtcgtt tgatctacca ccagttgacc cacggcattc
     2401 acattctcgc gcgtcggcgg tgcctgaaca tcggccggcg cggatttgag ccacagcgca
     2461 tgcgccagtc ccgtgccggt accggcagaa tcagggcggg ccgtcagcag ggacgacaac
     2521 caggcgcgac ccattcgtga ggtggtggat ttggccgtgg caatgccgac gacataggtc
     2581 cacgcctccg gggccgattc catcgggcgc gtgtaatact gcgccgcatt tttgagcacc
     2641 gcgcgcccat cgatggccgg ccgcgcgatg gcgatgaggc gcaatggtgc cagccgcaca
     2701 tatccgttga acatcacgcc ttccggatat tgtctggcca gcgattccca cagttgcgcc
     2761 agtgttgccg gttgttccag cctgaaactc gccacagcct cgcgctcacg tgaaacgtag
     2821 gcaaaaccgt tggactgaaa ataacgattg ctgaccgtgg cgaaatattt cggcgaatcg
     2881 gctgaacccc gacacttgga ttgagacgga ggcgggaaga gttaggaaga tgctggatat
     2941 gctgggaggg cctgaaaaga aggggtttgg ttaggtgaag agaaaacacg gccggtgatt
     3001 ttaggtttga gacgaaaaat tgaaatcaat aacttacgtg cgtctcagac acttggtttg
     3061 cgacgggttt ttcgtaactc attgaatttc cgttgggcgc ttccggcttt caaagtcgga
     3121 agtcggaagt ccgcttccga cttcggcgca acttccgact tcgagaaagc agcctaaaaa
     3181 tagatgtttc tgagaaaaac ggggaatatt agaaattgct aaagtcggaa gtgctgcttt
     3241 ttaaaattgc gaaaaagagg cgattttcag gttgagaggc aagaatcggg ggaaatatca
     3301 aaaaagcttg ccctgccgcc ggcgcacttc ttgctcacgc atggcggcca cgatctggta
     3361 gatacgctga agagtgacat tgttcttgcg ggccaggtca gcatggttgt ggccgttgaa
     3421 ttcgcgccag atttcgaggt cgcgctgggt gagcgcaaag agcgtgccct tcgggatgta
     3481 gaccagctgc ccgccccact cggttctgat gaattccgcc gcctcccggc cgatctcttt
     3541 cgccttttcc cgttccagcc cgcgcacgac cagggcctcg gtcagcttgt cggccagatc
     3601 ggcgagcagc tcggggtatt caacagcagc gttatcactc attaacttct cctttcccgg
     3661 gcttcgtggc ccctttaagc tgctccaggt gttgctcgac ccgcgagggg tccatgatct
     3721 tgccgatcgg tgctggctgc gatgtcgccg cgcggcgcgg gtggcgcttt tgctgctcga
     3781 ccgccgcctc cgcctggccc tcggtcttgg aggcgatccc ggagacgatc tcgaagaggt
     3841 aaccgtggct cttgagcggc agttggagct tgtcgcgccg ctggatcatc tgttcgagcg
     3901 cctcgcgcca gtaatggagg ggcgcgggcc aggcgcggcc atgtcgctcg acgcgcgccg
     3961 cttggatcgg atcgatgagt tcattcagta gcttcgccgc ccgatcccag ctcaaggccc
     4021 gttttgaggg ccggaaaaac gccatgtaac ggagcattaa atcaccgagc ggtgccggca
     4081 gtttcaatgc cgcagccacc gcctggcgcg cggcatcgtc cgtgagcgcg gcctcgatcg
     4141 cgtagcgtgc gaaacagcag gggcaggtga tctgcatcag aagccctcga agtccggcag
     4201 tttctcgaac tgatcttcgg tcaggtatac gtcttcaacg atgtactcac tcggctcgtc
     4261 cgactcggcg ataaaggctg cggcgccttt ctgtgtgtcg ataaagctgg cgctgttgca
     4321 ctcgttcagt cgaacgcgct tatagagctt cgtctgatag ttagggcagc tcggcgcgtg
     4381 ttcactcgcc ggaagcttcc cgccctgcgt ttcctcccac agccttacat gattcatgca
     4441 gcagtcgaac atgtcactcc cgcctcaaat taaacgcgac catcagattg agcctccgcg
     4501 cgcgcgcgag ccattgctga tatgtcttct ggtgctccgg gcagaggtgt ttatccggcg
     4561 caacctccag cgcgtgttcg gcgcaaaggt gcttgtcgca ggttttccca ttaccgagct
     4621 tccagtcgca ctgaaaggcg ccggcatgcg cgcaccaccg gcaacgctcc gcgcgccggc
     4681 cgcggccgca cacaatcgcc gtggtgccgt tttccagttt gatgtgacga caatccatca
     4741 ctttatggct ttcaatctcg gtgcggtggc gatgaatccg gtgccgatgc tgccgtcggg
     4801 attcgcctgt acacgcatgc ccagtccttc cagcactttt agatgctcga cctcgacctt
     4861 ggccgattca atcatcacct tggcaacatc ggcgatggcc ttggcgcggt cgatttccat
     4921 cggattctta tcgctcttca gactctcgat ggtctcgaac aggtggtcac gcagatcttc
     4981 gatgcggttc ttcatttctc agtctccggt tgatttgacg tttgagcgcg ccgcgcaact
     5041 ggatcacttc gcagagctct cgcgggtagc ggttccacat gacattgagc cgcgcgagat
     5101 ccgcgcggct gaccaggcac aggttgtcga gcacgatgcg atcgcggtcg ccgtcgatga
     5161 acttcacggc gtagccgcgc ggcaccgggc cgtaatgctt ttcccacagc agtatgtgcg
     5221 tcggtttcca gttgcgcgtc cacggcacgc agcggatatc gctgattttc gtgtaacgat
     5281 aaccatcgac aaggcgctcg ctgccaatcg gcttccattt gcggctcacc tggcctttct
     5341 tgaactgcgt ctcgcgcatg cggccggcgt accagccggg acggcgcaag cccttgttcg
     5401 ccggcgcgtg gcctttttga aaccggcacg cgaccgaccg ggtcgagagc atgtcacggc
     5461 gcagccggca ggcatccggg ctcgccaaat attccggcgt cttgcacagt ttgagcacct
     5521 gcgccttgct gtaaacccga tcgagcggac gacgaagcac gcgcgcgagg tgttccgtct
     5581 tgatgtgcgg ataaaaatgg cgcaggcgcc gcagctcggg ttcggtccag ggtttgatgg
     5641 tcactgcgtt ctcctgttgt gtcgttgagc atctttcacc agcgccgcga tgatgccctg
     5701 cagctgcgcg ggattgcaga aggcgacgcg ttcgacgtgg tacatgcgct tggccatgcc
     5761 gtcggcgtag gcccaggggc gcgcggcttc ggcgagcagg gcttcgattt tcttgagctg
     5821 cgggccgcgc tctccgctcg cgaggttgtg gggcttgccg ggatgtgcgc gcccgcgcgc
     5881 ggtctggaag ccgcaggctt taaggtgatc gcgcacgcgc cggcggccgg cttcgtcgag
     5941 gtcggcggcg ctgcgcacgc gcgcgaccgc ccacaacatc gagcgatagt cgctgttcgg
     6001 gtccttgtcg gcggcgtcca ggccgagcgc cttggcggcc aggtggatct tcgccagctc
     6061 gcggcggcgg gtatcattcg cggtgttcat ttcaccgatt tccaatgtgc gtcgatcgct
     6121 cgatcttgcg cttcatgttc accccaagtg actttattgc tccaaccgca ctggcagacc
     6181 gccaggctgt aggccggtct ttcgtgatgc cagccctccg tccgcatgga tacgacgtga
     6241 ccctctacgc caggcacggc gcttttgtta cgagcctcgg acatcttcga gcacctggtt
     6301 gatcttctga atgagccgct ccgtgcggtg gccgtaggcg acgatgcgcg cggttttgca
     6361 ggcttcctcg acgtgcggga ggatttcgag ccgggccgcg gcgatcgccg tctcggcatc
     6421 gggcgcgaac ggcagcttcg aatagatcga gctggaggcg agcgcgtaag cgagcgccca
     6481 gcgcggggcc ttctccagct ccgcggtgaa tttcatgatc tcgtcgtgca atacaaggcc
     6541 gttcatgcga tcttggtata ggtcggatgt ttcgtggtca tgtgccggcg caaattctcg
     6601 aagctgcgtt ggcagcacgg gcacacgccg tggccgatgc gcttcttcgt cttcgtgagc
     6661 tggcccttga gcgcgcgccg ccggcgctcg gtgatgcgcg cctcctgctg tgcccattcc
     6721 ttgcgcttgc gttcctgctc gacctcgcgc tccttctcgg cgagctgtgc tttcaaccgg
     6781 tcgatctgcg aagtgccgta accccaggag catttgcagt atgggcaatt ccaatatccg
     6841 ccttgctcga tcttctcctg gcggtagcgc agcgcgatcg cgtagatgcc gccgcacgag
     6901 ccgcacacct ggacgctgag gtctgcggac gaagtaacct gtgtacacgt tgcgagtgcc
     6961 atttaacttc tcctaaaaat cccgttcagt cgctcgtctt gccccagagt tccaggttgt
     7021 cgacgccgca tatctcggag ttctcttcga gcacttccgt gcccttgctc ttgaggaaga
     7081 acttccgatt gtggcgatgc cgggcggtgg cgatcgcctg ctccaggctg ttcgcctgca
     7141 cgttcaccgt gacgatcccg cgcaccagaa acttcacttc gtactccgcc atacagcctc
     7201 cttaaatcgc tcttaacagc gcccgtctcg tgggccagcc ggctcaggcc cgatgggagg
     7261 tggcccgtgt catgccggat cgtcctgagc tgccggtgtt attagcccca ccgccggctg
     7321 gggtagccaa gaagctcatg cgatacaccg cggctcatcg cacgctccgg cgcacgatcg
     7381 agagcgggtg atgtccggtc cgctcctgtg tctcgggcgg cgtcgggccg ccttccgcgc
     7441 gcaccaggtc gcgcagctcg aagctgcgca cgatgtgatc ttccagcgcg gccacgagcg
     7501 ccgcggcgtc gccgtcgtga aacgcgatca ggcagcgatc gaggttctcg atcagcgcgt
     7561 gcagtttttg ctcgtggcgt ttcaccgcag caccaccgcc ggggtggtga ataccgtgtc
     7621 gtcgttctgg cgacggcaac tcgggcacag ccggttgtgc gctccttgcg aggggaaatc
     7681 ctgacggcag cagagacagg ggcgcagggt ggatttggcg cgcgagtcgc gcgtcacgcg
     7741 gccggtgtag gctgatttcg cgggcatgcg cgcatcaacg cggcgcttgc ggcgaacggg
     7801 agaatggatg ccgagcgtgt aggccttgct gtggcaggac tgggccgtat ggttgacttc
     7861 ggcggcgatc tcctcgaacg agcagccgtc cgcgcgcatc tcgcgcaggc gcgccaactg
     7921 cgtgcgggtc cagggattgc gatcaggccg cggcacgctt gacctcctcg tccttgaaca
     7981 gcgcatcgac cagcttctcg atgtcggagt ccgcgacctt gaccaccacc tggtcgccgg
     8041 cctcgatgac gttgacgccg atgcgcttga gatcggccgc ggcgagattc gcgagcgcct
     8101 tcttgacgag cgtctcctcg gttttgatca ggacctcggc ctggtcttcg aggtgcttgc
     8161 ggatcagcgc gatggtcttc tcctcgtccg ggatctgggt ctcgcccttg cccttctgca
     8221 ggcccacctt cacgccgtgg aacaggcgcg tgcgcggctt ctcgaagagc tgcggggcgg
     8281 cctcgatcgc ggccttgagc ttctccttgg cgacggcggc gcgctcggcg ccgcgcttga
     8341 tcgccggcag cgccttcttc ttgagggcgc ggatcgcgct ctcaagggcc tcgacgtcgg
     8401 cggcgagctg ctggtactcg tccgcgtagg cccgggcgag ggtttcgatg tctttgaggt
     8461 cggtcatgct gcactcctaa gtggaattgc gacgatttca caccattggt ccgcgggtag
     8521 tgctgcgtac catgccgcat aagatggcga atattcgccg cgcgcgaggt ggatacccgt
     8581 cggccagcga cgatgggtga tgcgtttgat gtactcgcgc ggctcgccgc cgtgctcaca
     8641 agggcgataa tcgctgacga ttagaagccg cacgattttt ccgccgctgc gcagccatgc
     8701 gcgtacacaa cgccgggacc aggatgcgcg agtgcgcggt ttacagtttt tgagtgtggt
     8761 catgatgaac tcctaagtcg gcagatgcag ctgcccgcgc agatccggca gcgagatgcg
     8821 tttcatggcg gcgatgcctt taaggacgtg ggcgttttgg atgccgtaga tcgcatcggc
     8881 gggatggcgt tgcagtgatt ccatgaggcc tcctaacatg agagtgactt ggttccgtgg
     8941 atatggtcct cgcgctggac gcaggtgacg cgcatgccgt ccacgacggt ctcgaccagc
     9001 gtgagggttt cggtgggcgc cagcaggcgg cgcgattcgg tgtggctgcg ccattccgcg
     9061 tgcaggcagg aaatggtgag tatcccggcg aacacggcga agaagccggc ggtgacgcgc
     9121 cagccgcgcg ggctcatttc gcgcctccat tgatcttgga gtgcttgcag cgcggcccgc
     9181 cgacgccacg gcaggcttta tagagccgca cgcgggtgga gttggtcgcc tgaaacttcg
     9241 cgcgctgatg ctgcaggcag cggtgcagac cgatctcgcc gagcaccgga cagttgacct
     9301 tggcatcgag gtaagccccg cgcacggcgg cttccacctt ctttaaactg ccggtgtagc
     9361 ggtgccttaa cacctggtta ataaccgcgc tggagtagcc gatctcgtcg gcgacgatcc
     9421 gttgtgaacg aagatcgcac tgctcggcca gcacctgcac ccaatccggc agatcatcat
     9481 cccagcttga ggtcgcggcg cggacgttgg cgctgttcat gcgtcacctc ccgcctgcca
     9541 cacgaccttg ccctcgttca ggtccaccac ctgcagcacc tgctgcaaca gcggcgggcg
     9601 cggcccggtg ttgcgcgaat tcaggaagcg atagcgcagc agccggcccc cgggcggacg
     9661 cccctcggca tcacgcgcga gtgcggccac gtagccggcg gcgaccagcg cgcgcaggta
     9721 tacgcgcgca tcgcgctcgg tcacgcgaca ttgttcggtg ctggcgttca cggccaggtc
     9781 gatgacggtg aactctttca gcagtttcat cgtgcgccac agcgcgtcgc ggacgcagaa
     9841 accggccggc gcctggccgc gatggttcag caccggcgcc tcggggccga cgtcacgcac
     9901 cagatggtag agttcgccgg tcgtttcctg cttgatgaag ccggcgcgtt ccagcccatt
     9961 gagataatcg ctcaccaggc ggcgcggtag gccggttgtc agtacatcat caatggtgac
    10021 ttgcttatgc ttgcgcacga acgcccagat gcgatcgcgc caggctggcg aggaagttgt
    10081 agatttgagg cgcgcgggct ggaggctcat gcgtgcctct gcggcgcttc gccggtgtag
    10141 aactcgcggt tgccccactt cgccaggtcg atctctttcc aatgttcctg gctggcgagt
    10201 tcctgcgcac gctccaggtt gatgcagatg cggcgcacgt tggcgcgtgt gacttcgtgg
    10261 atgcgcgcga gcagatcgtc ggccaccttg gcgcgctggc agtacagcgt gcgcaggtgc
    10321 ttcgcgtccg ccatcgaggc cggctgcgcc ggcacccagt cgagcacgcg gttgtggaag
    10381 cgctcatagc ggcgcaggtt cacttcgagg cgctcctcgc cgatgatcag gatcggcgcg
    10441 ttgctgctct catagatgtc gcgcacgagt tcgaccaggt tgtggttcac cacggcgtcg
    10501 aactcgtcga gaatcaacgg ccggccagag agcaccaggt gctcagcgac cagattagtt
    10561 agctcgggaa ccgtgccgcg tggcgacaca cccatctcaa gcagaattcc ttctagcagg
    10621 gctttcttat gccaggagga tttgacctcg acgtaatacg cgcggtagtg gttcgcggca
    10681 taggccgatg acgccgattt gccccaaccg gaatgcccat aaaacgccac gatcccgggc
    10741 agatgcgact ggcgcttcat cgcgatctcc attgcgcgca tacagatcgc gacgttcgcg
    10801 agcggcgcgg tggtattgac cgactgttcc tttttcgaca tagtcacctc tccagttgta
    10861 ctgcggtaag gccgcgctca ggcccatcct gggcgcggtt ttttatttcc tatcccgcct
    10921 cgccggcctc aagcccgaac gattcatgga agttcttctg cgcgcggtac tcggcgcagg
    10981 tctgatagct cgcgagccat tcgcggtcgc gtccgtccac cggcaagccc tcatgcagcc
    11041 gccgctccag cgagcgagcg cgcgcgtagc ggctctcctc ggtgtcattc agctcgatca
    11101 cctgcggctg tgtccgtgcg gcgacgagtt gctcctcgat ggcgtgctcc tcgg
//
LOCUS       DBNGZE010000407         3165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_751272, whole genome shotgun sequence.
ACCESSION   DBNGZE010000407 DBNGZE010000000
VERSION     DBNGZE010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_751272"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="AC16FV_10475"
     CDS             complement(<1..147)
                     /locus_tag="AC16FV_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HIR2850516.1"
                     /translation="MLPIQKLLSRIRWDPRFRQGRFTLGYFDRVARNIVVVPFDAIRF
                     PSGAP"
     gene            complement(171..413)
                     /locus_tag="AC16FV_10480"
     CDS             complement(171..413)
                     /locus_tag="AC16FV_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850517.1"
                     /translation="MSTPDFDIRVECYAGHRGEEEPRRFWLGERPVEVAEILDRWLAP
                     EHRYFKVRGDDGATYILRHDTQADRWELTLFESRPG"
     gene            complement(442..1074)
                     /locus_tag="AC16FV_10485"
     CDS             complement(442..1074)
                     /locus_tag="AC16FV_10485"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR2850518.1"
                     /translation="MGKLVIKFQGKSIGDVNLRLGDMTIGRHSNCDIVLSNDKSVSGK
                     HAVIKTVGRKSTIEDLGSTNGTFLVSGRITRHELRHGETIVIGSHELIYRDDLALDTP
                     MFGKGPGAGAAAADSHDKTTIISMRAQLIAVEGKEKGKNVPLVKKETLLDNPGKNPAR
                     IYHGPDGYVLHAQVGPGEPRLNDKPVQPGGQMLENGDIIEVAGTKFQVVI"
     gene            1359..1793
                     /locus_tag="AC16FV_10490"
     CDS             1359..1793
                     /locus_tag="AC16FV_10490"
                     /inference="COORDINATES: protein motif:HMM:NF017954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF995 domain-containing protein"
                     /protein_id="HIR2850519.1"
                     /translation="MLRSILASVLLALPMAAQAGDLLLSDLKAQNGVQLSADELKQLM
                     PGVKVVSYTAAGSTRRWKNEPSGKFVASSDVRGDVHKSVVSTTAQGTWHVGDNGTYCV
                     TLEWPRKSESWCRYIFKVGDKYYGVKSLADGTSKAFEFEFSK"
     gene            complement(1803..2555)
                     /locus_tag="AC16FV_10495"
     CDS             complement(1803..2555)
                     /locus_tag="AC16FV_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR2850520.1"
                     /translation="MSQVQLRFYEELNDFLPPERRKIAFAHELSRRTSIKDLIESFGV
                     PHTEVEVILVNGRSVDFSYIVQAGDRISVYPMFERLDVSPLLRLRDKPLRHPRFVVDV
                     NLGQMARYLRLLGFDVVYRNSLTDREVAWIASAERRIVLTRDRALLQHKIITHGYFVR
                     AVKPREQVKEILTRLDLYRELHPFTRCLRCNGELEDVDKETVLHQLEPKTRKYYERFR
                     RCKACGQAYWKGSHFNRMEKLCDYFMAPSGPA"
     gene            2703..2963
                     /locus_tag="AC16FV_10500"
     CDS             2703..2963
                     /locus_tag="AC16FV_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5062 family protein"
                     /protein_id="HIR2850521.1"
                     /translation="MKKLKHEAELVKGAILAGMKYGEERGAVIFEPNDSASEKILFIY
                     RLLVHDKAIQPLPEDQVSQQSMRHKLAIWYSKQLPPDHPLLK"
BASE COUNT          675 a          894 c          909 g          687 t
ORIGIN      
        1 cggcgccccg gatgggaagc ggatggcatc gaacggcact accacgatgt tccgcgcaac
       61 gcgatcgaaa taaccgagcg taaaccgacc ctggcgaaag cgtgggtccc agcgaatccg
      121 gctcagcagt ttctggatag gcagcatgta tgggcgtgag actctgcgga tcagcccggg
      181 cggctttcaa acaaggtgag ttcccagcgg tcggcttgtg tgtcatgacg cagtatgtag
      241 gtcgcgccat cgtcgccacg aaccttgaag tagcggtgct cgggagcgag ccagcgatcg
      301 agaatctccg caacctcaac cgggcgctca cccagccaga aacgccgcgg ttcctcttca
      361 ccgcgatgcc cggcgtaaca ctccacacga atatcgaaat ccggagtact cacacgagcc
      421 ggccctccgg gttacgggcg actatatgac gacctgaaac ttcgtccccg cgacctcgat
      481 aatgtcaccg ttctccagca tctggccgcc gggctgcacc ggtttatcgt taagcctcgg
      541 ctcgccaggg ccaacctgcg cgtgcaatac gtagccatcg gggccatggt aaatccgggc
      601 cgggttcttg ccggggttgt cgagcagggt ttcctttttc acgagtggca cattttttcc
      661 cttttctttt ccttccaccg cgatcagctg cgcccgcatc gagatgatcg tggtcttgtc
      721 atgcgagtcg gccgccgcgg cgccggcgcc cgggcctttg ccgaacatgg gcgtatccag
      781 ggccagatcg tcgcgataaa tcagctcgtg cgaaccgatg acgatcgtct cgccgtggcg
      841 aagctcatgc cgtgtaatgc gtccgctcac gaggaaggtt ccgttcgtgc tcccgaggtc
      901 ctcgatggtg gatttgcgcc cgaccgtttt tatcacggcg tgcttgccac tgacgctctt
      961 gtcgttgctc agcacgatat cgcagttgga atgacgaccg atcgtcatgt cgcccagccg
     1021 caggttcacg tcgccgatcg actttccctg gaatttgata acgagtttgc ccatgtgttg
     1081 ccgattttcc gctcctgttg gttctacaac tgacgaaacc ggtcctgccg ttccgtagga
     1141 tacaaaagga aacggagata gcaaactgtg tccccggaat taatggtccg cacgaccggg
     1201 aatttttgaa tctcctgtgc ttcaggcatt tactgcagaa attgaccccg ctcaacgaca
     1261 aaagggtggg ggcgtgaaac attccccatc aggcagaaca gggcctatgt tccggttgta
     1321 caccaaaatt caacatccag attacggcag gaggttttat gctgcgttcc atccttgcat
     1381 ctgttttgct ggcacttccc atggctgctc aagcgggcga cttgttgctg agcgacctga
     1441 aagcgcagaa tggtgttcag ctatccgccg acgagctgaa gcagctcatg ccgggcgtca
     1501 aagtcgtcag ttataccgcc gccgggagta cccgccgctg gaagaacgaa cccagtggca
     1561 aattcgtggc atccagcgac gttcgtggtg atgttcacaa gtcagtggtg tcgacaaccg
     1621 ctcaaggcac atggcatgtg ggcgacaacg gaacctattg cgtcacactg gaatggccca
     1681 gaaaatccga gagctggtgc cggtacatat tcaaggtcgg tgataaatat tacggcgtca
     1741 agtcgttggc cgacggaact tcaaaagcct tcgagtttga gttttcaaaa taaccactgg
     1801 cattatgccg gtcctgatgg cgccatgaag tagtcgcaca gtttctccat gcggttaaag
     1861 tgactgcctt tccaataagc ctgcccacag gctttgcagc ggcgaaagcg ctcataatac
     1921 ttcctggtct tcggctccaa ttggtgcagc acggtttcct tgtctacgtc ttccagttcg
     1981 ccattacagc gcaggcagcg ggtgaagggg tgcagttcgc ggtagaggtc caggcgcgtg
     2041 agaatttctt ttacctgctc gcggggtttg acggcgcgca cgaaatagcc atgcgtgatg
     2101 attttgtgtt gcaacagggc gcggtcgcgc gtcagcacga tgcgcctttc cgcgctcgcg
     2161 atccacgcca cttcgcggtc ggtgaggctg ttgcggtaca cgacgtcaaa acccagcaac
     2221 cgcaggtagc gtgccatttg cccgagatta acgtcgacga cgaagcgggg gtggcgcagg
     2281 ggcttgtccc gcagccgtag cagcggactg acatccagcc tctcgaacat gggatagacg
     2341 ctgatgcgat cgcccgcctg cacgatatag gaaaaatcca cggaccggcc gttgaccagg
     2401 atcacttcca cctcggtgtg cggcacgccg aaggattcga tcaggtcctt gatcgaggtg
     2461 cggcgcgaga gctcgtgggc aaatgcgatc ttgcgccgct ccggcggcag aaaatcattc
     2521 agctcctcgt agaagcgcaa ctgcacctgt gacatggacg agacgcctcc tgagaggatg
     2581 gccgattcac tgcaataaat gggggcacag ggcttattaa gcaacctgaa tgaagtaagc
     2641 gccacgttgc tcaaaacggc gtatggcgtt atgtgcaata ggtgtacatt accgggatcg
     2701 tgatgaaaaa actgaaacac gaggccgagc tggtgaaagg agcgattctc gccggcatga
     2761 aatacggcga ggaacggggc gcggtgattt tcgagccgaa cgattccgcc agcgagaaga
     2821 tcctgtttat ttaccggctg ctggtgcacg acaaagccat tcagcccctg ccggaagacc
     2881 aggtctcgca acaatccatg cgccataagc tagcaatctg gtattccaag caactcccgc
     2941 cggatcatcc gctgttaaaa taagtttttc tgcgcacacc cggcaagcct gactgattga
     3001 ctcagaaaaa ccgtgacgcg gcttccgggt cgggcggtat ttcctgcatt ggcaatgatc
     3061 gagtttgggt ttcggattat tctttgacgc tggctttccc gtcatgatca aaggcgaacg
     3121 gttcaacagt atttcgcatc tggtcggcgc cgcgctggcg ctggc
//
LOCUS       DBNGZE010000408         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1236816, whole genome shotgun sequence.
ACCESSION   DBNGZE010000408 DBNGZE010000000
VERSION     DBNGZE010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1236816"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1163)
                     /locus_tag="AC16FV_10505"
     CDS             complement(318..1163)
                     /locus_tag="AC16FV_10505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2850522.1"
                     /translation="MPFGRGVKWALSLAVALAGGWSGAVQPVMAAQDRLETSVCGIVR
                     EPLAFWLFRRAAGTPDAQRLAGIRDIERIRFETLDKRTLGGYRLRAGGNPRGYLLVAQ
                     GNAMLADQIMGELQIFRDRGFDVYVYDYRGYGLSGGSSRLRAIVSDYRELVAHLNRQN
                     YRRRALYGMSMGGVVLLNAVGARDDFSALVVDSSPSRISHLGCPEEFDPVNHLPANSS
                     RIKIIAGGRDRVVRPEEMQELALAGGLRGAQVVQRAEFAHPFQDATAEIHRQRLKEVA
                     DFIER"
     gene            complement(1168..1827)
                     /locus_tag="AC16FV_10510"
     CDS             complement(1168..1827)
                     /locus_tag="AC16FV_10510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850523.1"
                     /translation="MDINAVQKVYRRYAPAYDFYFGAMFQPGREEIIARMDCRPGDRI
                     LEVGVGTGLSLSLYPRHVEVTGIDLSREMLVRARVRRRRLRLHQVVALRLMDAEHMQF
                     ADNSFDKVVAMYVVSVTPHPARMMDEMRRVCKPGGQLFIVNHFQHPNSLVRGMERLVS
                     PFSRVMGFRPDYSLEHFIRETRLDVATQVPVNALGYWTLLQCRNNKHSKAGVSPMHAI
                     A"
     gene            complement(1899..2246)
                     /gene="hpf"
                     /locus_tag="AC16FV_10515"
     CDS             complement(1899..2246)
                     /gene="hpf"
                     /locus_tag="AC16FV_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR2850524.1"
                     /translation="MIMQIPLQITLKDMPQSEAVESRIREKAEKLTRFHERIINCRVV
                     VESPQRHQHQGKLYSVHIDLSVPGAELVANRAQDEDVYVAIRDAFGAITRQLEDFTRR
                     QRGDVKTHSAPGS"
BASE COUNT          490 a          732 c          667 g          425 t
ORIGIN      
        1 tcgattctgc gcgacgggca gtggtttccg cgccgcggtg cgatcagcgt aacggccggc
       61 gcggcgcttt ggccggatgg ggcagactgg tccgccgcga tcaaactgcg tgacgcggca
      121 cgcgcggaaa tcctcaaaca ctgcggcgaa cccgatctgg cctagaaact tctcaaaaac
      181 acctgaaacg cagatgaacg cagataatga agagataaac gcggatcatc atatgtgaat
      241 tcttcgtata tctgcgttca tccctgaaaa taaatctgcg ttaatccgcg tttaaagtat
      301 ttcgagacag gttcttatca acgctcgata aagtccgcga cttcctttaa acgctgccga
      361 tggatctcgg cagtcgcgtc ctgaaacggg tgcgcgaact ccgcgcgctg cacgacctgc
      421 gcgccgcgca gccctcccgc cagcgcgagt tcctgcattt cttcgggacg taccacgcga
      481 tcgcgtccgc cggcgatgat cttgatgcgg gagctgttcg ccggcaggtg attaacggga
      541 tcgaactctt ccgggcaacc cagatgcgaa atccggctcg gcgaggaatc caccaccaat
      601 gccgaaaaat cgtcgcgcgc acccacggca ttcagaagga caaccccacc catcgacatg
      661 ccgtataacg cgcggcggcg gtaattctgg cgattcaggt gcgcgacgag ctcacggtaa
      721 tcggaaacga tggctctcag ccggctgctg cccccggaaa gaccgtaacc gcgatagtcg
      781 taaacataaa catcgaaacc gcggtcgcgg aagatttgta gctcgcccat gatctgatca
      841 gcgagcatgg cgttgccttg cgccaccagc agatagccgc ggggattccc tccggcgcgc
      901 agccggtaac ccccgagtgt gcgtttatcg agggtctcaa atcggatgcg ctcgatgtcg
      961 cggatgcccg cgagcctttg cgcgtccggc gtgccggcgg cacgccggaa aagccagaac
     1021 gccagcggct cccgcacaat tccgcaaacg ctggtttcaa gtctgtcttg cgcggccatc
     1081 accggttgca ccgccccgct ccatccgccc gcgagcgcca cagccagcga caacgcccat
     1141 tttactccac ggccgaaagg catagcctca ggcgatcgca tgcatcgggg aaacgcccgc
     1201 cttggaatgt ttgttgttgc ggcactgcag cagggtccag tagcccagcg cgttcacggg
     1261 aacctgtgtc gcgacatcca gccgtgtctc gcgtatgaaa tgctccagcg agtaatcggg
     1321 gcgaaatccc atcacgcgcg aaaacggcga aaccagccgc tccatcccgc gaaccaggga
     1381 attggggtgc tggaaatgat tcacgatgaa aagctggccg ccgggtttgc acacccggcg
     1441 catctcatcc atcatccgcg ccggatgcgg tgtgaccgac acgacataca tggccacaac
     1501 cttgtcgaaa ctgttgtcgg caaactgcat gtgctcggcg tccatcaggc gcagcgccac
     1561 cacctgatgc agccgcaggc gtcgccggcg cacacgggcg cgcaccagca tttcgcggga
     1621 gagatcgatg cccgttacct cgacgtgacg cggataaagc gacagggaaa ggccggtgcc
     1681 cacgccgacc tcaagaatgc ggtcgccggg gcggcagtcc atccgggcaa tgatttcctc
     1741 gcggccgggc tggaacatgg ccccgaaata aaaatcatag gccggagcgt aacgtcgata
     1801 aaccttctga acggcgttga tatccatgcg catgactcct catggccggc cattccccta
     1861 ccccgcccct gcaaacgccc gaaacccggg cggtacgttc agcttccggg cgcggaatga
     1921 gtcttcacgt cgccacgttg gcgccgcgtg aaatcctcca gctgccgggt gatggcgcca
     1981 aaggcgtcgc gtatagcgac atagacatcc tcatcctggg cgcggttcgc caccaattcc
     2041 gcaccgggca cggagagatc gatatgaacg ctgtagagct tgccttgatg ctgatgccgt
     2101 tgcggcgatt ccaccaccac gcggcagttg atgatccgtt cgtggaagcg ggtgagcttc
     2161 tcggccttct cgcggatacg ggattcgacc gcctcggact gcggcatgtc tttcaaggta
     2221 atctgcaacg gaatctgcat gatcatgctc ccctttggtg cataggtaac tagtatgcgt
     2281 gactcccttc gcgcgaatca catcctgagc gaca
//
LOCUS       DBNGZE010000409         6214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1515070, whole genome shotgun sequence.
ACCESSION   DBNGZE010000409 DBNGZE010000000
VERSION     DBNGZE010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1515070"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="AC16FV_10520"
     CDS             <1..1126
                     /locus_tag="AC16FV_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850525.1"
                     /translation="LALCGLIVMMVIAILKKKQIPLLSFAVLWFLVAHLLESSVFGLE
                     VAYEHRNYLPAYGVLFAISYLYVCVSRHWNTRLRFIIAVSVIFTLGFATWTRAHTWKD
                     VYSIAEAEVTNHPESPRANELAARVNAFDKSNLPAAIRYTIQGLKIAPDEAGFHINLH
                     LFLVLLSSQIDQGMKSTDMKNLKNADIHIDGLLPEIIATVNNNHVQLVYPLSTINTVE
                     KLLETRPITVHTLAALDAIGRCIMEKPDICRQTTNQVLKWHVIASDNPDTTKAYHALI
                     LNNTAELYAHASDYTTAMIYIDKAIQAVPDVLFYRLKKTEYLVKQGRLDEAQSLLADI
                     HKTGLKNDPRFLANRAMVESVQKKYEEAIRNRRQQTLSSQ"
     gene            1295..2173
                     /locus_tag="AC16FV_10525"
     CDS             1295..2173
                     /locus_tag="AC16FV_10525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2850526.1"
                     /translation="MDKSKNKPASVSIVIPAKNEEKGLEELLPKLIRLYPKAEINVVN
                     DGSTDATVRICQIHGVKTVNHPYSMGNGAAVKSGGRAATGNILVFMDADGQHNAEDIQ
                     RLLDKLNENYDMVVGARDSGSQTSIGRSYANRIYNWLASWMVGTRIDDLTSGFRAVRA
                     DKFRKFLYLLPNGFSYPTTITMSFFRAGHAVAYVPIVASRRQGKSHIRVMHDGVRFLL
                     IIFKIGTLYAPLKLFFPVSLGFFFTGLAYYLYTFLMFQRFTNMSALLFITSILVFLIG
                     LVSEQITALNYKDSEK"
     gene            2163..2969
                     /locus_tag="AC16FV_10530"
     CDS             2163..2969
                     /locus_tag="AC16FV_10530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850527.1"
                     /translation="MKSKTMVDTRGISILDTLVTPAPRYLMRLALIEQMAARLPMNIR
                     SFLEIGPGMGDLSLYLGRRFPEADGILLDFSSDCIDILRQRTAHNPRLQLKTGDFMTM
                     PHEERHDLIIACEVFEHINDDITAFRIVSELLHPGGHFIFSAPAFMRKWQRADEYAGH
                     YRRYERAELIEKFTASGFRIHALWSFGFPVTQLLYPARQLYYGMSRRGQHLSKEDATK
                     RSGIERPLIGRNRALLLANLLRPFYFLQNQVKNTDLGDGFLVLAQKDRPV"
     gene            3485..4198
                     /locus_tag="AC16FV_10535"
     CDS             3485..4198
                     /locus_tag="AC16FV_10535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850528.1"
                     /translation="MNAGYFAETFGLAPSKIMSLPLSIDADSFRPAPYQPHGDSCTVL
                     FIGTFVPLQGVDVIARAALALESRQNVRFRLIGSGQTADAVARIFANRRPANLEWITR
                     WMDSESLAREIRDADICLGIFGASAKTQRVWPLKNYAYMAVGRAIITSDTPQARRMLQ
                     QTDAVPFMTVPQGDPVALASAITELAGDPERRRRYAANARRYYEKHLSPQTALEQIIL
                     QFTHASNIVIPPHSRNLQS"
     gene            4195..5145
                     /locus_tag="AC16FV_10540"
     CDS             4195..5145
                     /locus_tag="AC16FV_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2850529.1"
                     /translation="MKHLFLIFKLIITASLCFWLYTRIDFHALRESLARLGALPIVIG
                     VLLHVAVVFLSASRWWMLLTHTHAAIPFFKAFPSYYLGVFFNNFLPTGLGGDTVRILH
                     MRAHGVSMKSLVSASVVDRVAGFATIFMVGMIGVLVSRELRIPGHTKSVLVAVFLAGL
                     LLAGWILSSHSGRLIESLARKYRHTRIRGWILDVILTCYSYRAEKKRIAFAVVISAIG
                     QGLVILTYYLIGRGLGLNLSLTTYLVAIPAVFLAASLPISIGGLGIREVTLVCMLAAA
                     GAGLQTAVNLSLVYLIVFWISTLPGALVPLLSRTSKIASS"
     gene            5142..>6214
                     /locus_tag="AC16FV_10545"
     CDS             5142..>6214
                     /locus_tag="AC16FV_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850530.1"
                     /translation="MSERAEQIRRHFDALSADAGRSTRRNRHYHREQTHYFRYLVAEN
                     KRVLELGCGNGDLLNALKPSHGVGIDISPGMVELARKKYPQLTFHVGDGNRLDQLPPE
                     TFDYIILSDFVGYLDDIQACLEVLHPFCGPHTRLIISYYNFLWEPVLKLAELLRLKTP
                     TPEHSWLTPDDMRNLTSLADYQVVKTERRLLFPIYIPVVSWLLNHIGTLPGINKACLC
                     HYTVARPAPVRATNDRSVSIVIPCRNEKGNVEPAIQRLPRFGTHQEIIFVDGHSTDGT
                     PEEIRRVQTLYADRDIKLLMQDHTGKGDAVRKGFQEARGDVLMILDADLTVPPEDLPK
                     FYRAIVSGKAEFLNGCRLVYPMEG"
BASE COUNT         1381 a         1803 c         1620 g         1410 t
ORIGIN      
        1 tctggcgctc tgcggactga tcgtcatgat ggtgattgcg attctgaaaa agaaacagat
       61 cccgctactc agttttgccg tactctggtt tctggtggca catcttctcg aatcaagcgt
      121 gttcggactc gaagttgcct acgaacaccg caattacctg ccagcctatg gcgtcctttt
      181 tgccatctcc tacctgtatg tatgtgtcag cagacactgg aatacccgcc tgcgtttcat
      241 tatcgccgtg tctgtcatct tcacgctcgg cttcgccacc tggacacggg cgcatacgtg
      301 gaaggatgtc tattccatcg ccgaagctga agtaacgaat catccggaat cgccacgggc
      361 caatgaactg gccgcgcgcg tgaacgcctt cgacaagagc aatttgcctg ccgctatccg
      421 ttacaccatc cagggattga agatcgcacc tgacgaagcc gggtttcaca ttaatttgca
      481 tctttttctg gtcctgcttt catcacagat cgatcagggc atgaaatcca ccgacatgaa
      541 aaatctgaag aacgcggata ttcatattga cggattgctt cccgaaatta ttgcaaccgt
      601 caacaataat catgtgcagc tggtttatcc tctatcgaca ataaataccg tcgaaaaact
      661 tctggaaacc agaccaatca cagtgcatac actggcggca ttggatgcga taggccgatg
      721 cataatggag aaacctgaca tctgccggca gacgacaaat caagttttga agtggcatgt
      781 catcgcgtcg gacaatcccg ataccactaa ggcctatcat gccttgatat tgaataatac
      841 ggcggagctg tatgcgcatg cgtccgacta cacaacggcg atgatttaca tcgacaaggc
      901 gatacaggcc gttcccgacg tgctttttta caggctgaag aaaacagaat atctggtgaa
      961 acagggacgt ctggacgagg cacaatcgtt actggctgat atccacaaaa ccggactgaa
     1021 aaatgatccc cggttcctcg ccaaccgggc aatggtggaa tccgtacaaa agaaatacga
     1081 agaggcgatc cggaatcgac gtcaacagac gctgtcgagc caatgacatc aaccatagcc
     1141 ccttgacttg gcgatgggga gaaaccgttc ccgaagcgaa tttgcccgga atctatagtc
     1201 tataattagc cgggaagcca aggccattca cggggaaggc ctgaaataac tagcgtattg
     1261 cacggggacc aaggggcgtc atcacgatat ggacatggat aaatccaaaa acaaaccggc
     1321 gtccgtgtcg atagttatcc ccgcaaagaa tgaagaaaag gggcttgagg aactgctgcc
     1381 caaacttatc cggctatacc ccaaggccga gatcaatgtc gtcaacgacg gctccactga
     1441 cgcaacggta cggatttgcc agatacacgg agtcaaaaca gtcaaccacc cctacagcat
     1501 gggcaatggc gctgccgtca aatcgggagg gcgcgccgcg accggcaaca tcctggtttt
     1561 catggatgct gacggccaac ataacgcaga ggacatccag cgcttgctgg acaagctcaa
     1621 cgagaactac gacatggtcg ttggggcacg cgacagcggg tcacaaacca gcattggcag
     1681 atcctatgcc aacagaatat ataactggct ggcgagctgg atggtgggaa caaggattga
     1741 cgacctgact tccgggttca gggcggttcg ggccgacaaa ttccggaaat tcctgtatct
     1801 gctgccgaac gggttttcat accccacaac cattaccatg agtttttttc gcgccggtca
     1861 tgcggtagcc tatgtgccga tcgtcgccag ccggcgccag gggaaaagcc acatacgcgt
     1921 catgcatgat ggcgtccgct tcctgctgat tatcttcaag atcggcacgc tgtacgcgcc
     1981 acttaagctg tttttcccgg tcagcctcgg atttttcttt accggccttg cgtattatct
     2041 ctataccttc ttaatgtttc agcgcttcac caacatgagt gcgctgctct tcatcacctc
     2101 catactggtg tttcttatcg ggctggtttc ggaacaaatt accgcgctga actacaagga
     2161 cagtgaaaag taaaacgatg gtcgacaccc gggggatatc catactcgac acgctggtga
     2221 cacccgctcc gcggtatctg atgcggttgg cgctgatcga gcagatggcc gcgcgtttgc
     2281 ccatgaacat ccggtccttt ctcgagatcg gccctggcat gggcgacctg agtctctacc
     2341 tcggccggcg tttcccggaa gccgacggaa ttttgctcga cttctccagc gactgcattg
     2401 acatcctgcg acagagaacc gcgcacaatc cgcgtttaca actgaagaca ggcgacttca
     2461 tgactatgcc gcatgaggag cgccacgacc ttatcattgc ctgcgaggta ttcgagcata
     2521 tcaatgacga cataacagcg tttcggatcg tctccgagtt gctgcatccc ggcggccatt
     2581 tcattttctc ggcgccggcc ttcatgcgaa agtggcagcg cgccgacgaa tatgcgggcc
     2641 attaccggcg ttatgaacgc gccgagttga tcgaaaaatt caccgccagc ggctttcgca
     2701 tccatgcctt gtggagtttc ggtttccctg taacacaact cctctaccct gcgcggcagc
     2761 tgtattacgg catgtcacgg cgtggacaac acctgtcaaa agaagatgcc accaaacgca
     2821 gcggcatcga acggcccctc attggcagaa accgagcgct attgctggcg aacctgcttc
     2881 gccctttcta ctttctgcaa aatcaggtca agaacaccga cctcggcgat ggatttctgg
     2941 tcttggcgca gaaggaccgc cccgtgtgaa ccctcaacag gaagaaaaac cggaaagacc
     3001 ggaacccgtg gctgtcacca ttctgggcgt gcacgtaaaa tccacaggat atcccaatgt
     3061 cacctttcgt ctgcgcgatc ttgagcgtgc cgtgccgttg cgaacacagg aaataaattt
     3121 tcccttccgc agcgccacac cgcatgacga tatgcggttg ctcgcctgga aaccgttgaa
     3181 attgatgtgg tcggcactcc gactggttta tgcgcacctg tacattctgc tggcatatat
     3241 ctggcacgga cgtccacgcc ggatatacgt cccctatcct tcggctatcg tgcttttctg
     3301 tctgtcgctg ctgccaaaat cctggcgtcc cgaatatgtc gtcgcggact gtttcatctc
     3361 cctgtacgac accgttataa ccgacaggaa actcctgtcc cctcattcac tggctgcgcg
     3421 cgtcctgagg ctcgtggaat cgcgggccta tcggaccgcg gacaggataa tcgttgatac
     3481 cgatttgaac gccggctatt ttgcggaaac ttttggcctc gcgccttcga agatcatgtc
     3541 tctgcccctc agtattgatg cagacagttt ccggccggcg ccgtaccaac cgcatggtga
     3601 ttcctgcacc gtgctgttta ttggcacctt tgtcccgtta cagggggtgg atgtcatcgc
     3661 gcgcgcggcg ctggcgctgg aatcgcgcca aaatgtgcgc ttccgtctca ttggttctgg
     3721 tcagacggca gatgccgtag cgcgtatttt tgcgaatcgg cgtccggcaa acctcgaatg
     3781 gattacccga tggatggaca gcgaaagcct tgccagggaa atccgggatg ccgacatctg
     3841 tctcgggatt tttggcgcga gcgcgaagac acagcgtgtc tggccgctca agaattacgc
     3901 gtatatggcc gttggacgcg ccatcattac cagcgataca ccgcaagctc gacgcatgct
     3961 gcaacagacg gatgctgttc cgttcatgac cgtgccccaa ggtgatccag tggcactggc
     4021 ctccgccatc accgaacttg ccggcgatcc cgaacgtcgg cggcggtatg cggcaaacgc
     4081 ccgccgctac tatgagaagc acttgtcccc gcaaacagcg ctggaacaga tcattctgca
     4141 attcacgcat gcctcgaata tcgtgatacc gccgcattcc cggaatctgc aatcgtgaaa
     4201 cacctcttcc tgatattcaa actgatcata acggcctcct tgtgcttctg gttatacacg
     4261 cgcatcgatt ttcacgccct gcgggaaagt ctggcacggc tcggcgcgct gcccatcgtc
     4321 atcggggtat tactgcacgt ggcggtcgtt tttctcagcg cgtcgcgctg gtggatgctc
     4381 ctgacgcata cgcatgccgc catacccttc ttcaaggcat ttcccagcta ttatctcggg
     4441 gtgttcttca ataattttct gccgacgggg ctggggggag ataccgtgcg cattctgcat
     4501 atgcgggccc atggcgtgag catgaaatca ctcgtgagcg cgagcgtggt ggacagggtt
     4561 gccggatttg cgaccatttt catggttggc atgatcggtg tgctcgtttc ccgggagttg
     4621 cgcatacccg gccacacgaa atcagttctt gtcgctgtct tcctggccgg cctgttgctg
     4681 gccggctgga tattatcgag ccacagcggt cggctcattg aatcgcttgc gcgcaaatac
     4741 cgtcacacgc ggatacgcgg atggattctg gacgtcatcc tcacctgcta ttcctatcgc
     4801 gcggaaaaaa aacgcatcgc gtttgccgtt gtaatatcag ccatcgggca aggcctggtc
     4861 atcctgacct attatctgat cgggcgcggc cttggcctga acctttccct caccacctat
     4921 ctcgtcgcga taccggcggt ttttctcgcc gccagccttc cgatttctat cgggggactc
     4981 ggcatcaggg aggtcaccct ggtgtgcatg cttgccgccg ccggcgcggg cctgcagacc
     5041 gctgttaacc tgtccctcgt ttatcttatt gtattctgga tatccacctt gccgggcgct
     5101 ctcgtgccat tactcagccg caccagtaag attgcctcct catgagcgaa cgtgccgaac
     5161 agataaggcg ccactttgac gcattgtccg ccgatgccgg cagatccacg cggcggaatc
     5221 gccattatca ccgcgagcag actcattatt tccgctatct ggtcgccgaa aataagcgtg
     5281 ttctggaact tggttgcggc aacggcgatc tgttgaacgc cctcaaaccg tcacacggcg
     5341 tcggtataga tatcagtccg ggcatggtgg agttggcgag aaagaaatat ccccagctga
     5401 cgttccatgt cggggacggc aatcgactcg accagctgcc gccggaaacc tttgactata
     5461 ttattctgtc tgacttcgtg ggttatctgg acgacatcca agcatgcttg gaggtcctgc
     5521 atccgttctg tggaccccat acccgtctca ttatttccta ttacaacttc ctctgggaac
     5581 cggtcctgaa actggccgaa ctgctgcgcc tgaaaacgcc gacgccggag cacagctggc
     5641 tgacgccgga cgacatgcga aatctgacgt ccctcgccga ctaccaagtg gtgaaaaccg
     5701 agcgacgtct gctttttccc atctacattc ccgttgtttc gtggctgctc aatcacattg
     5761 gaacattgcc gggaatcaac aaggcatgtc tttgccatta caccgtagcc cgtcccgcgc
     5821 cggtgcgcgc gacaaacgac cggtcggtct ccatcgtcat tccctgccgc aacgaaaaag
     5881 gcaacgtcga gccggccatc cagcgcctcc cgcgcttcgg aacacaccag gaaatcatct
     5941 tcgtcgatgg acactcgacc gacggcacgc cggaggaaat aaggcgtgtg caaacgctct
     6001 atgccgaccg cgatatcaag ttgctgatgc aggaccatac cggcaagggc gacgccgtgc
     6061 gcaaagggtt tcaggaggcc cgaggcgacg tgctgatgat tctggacgcc gacctcaccg
     6121 tgccgccgga ggatctgcca aagttctacc gcgccattgt cagtggaaaa gccgaattcc
     6181 tgaacggatg ccggctggtt taccccatgg aggg
//
LOCUS       DBNGZE010000410         2437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1376349, whole genome shotgun sequence.
ACCESSION   DBNGZE010000410 DBNGZE010000000
VERSION     DBNGZE010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1376349"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1525
                     /locus_tag="AC16FV_10550"
     CDS             392..1525
                     /locus_tag="AC16FV_10550"
                     /inference="COORDINATES: protein motif:HMM:NF018439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HIR2850531.1"
                     /translation="MKYRRYPSIFCILAGISRQIFLGALVIGIMAPVSAAGPEEYLRG
                     YVDSLLDSRFPGLGLRSQIISADGAVKLMSRTCLGPSQKRDVLYLLTATGRVKNVQWD
                     LSTDCDKSDAVEQDKAGTEREPETPVDVYALPEQELFAPLLADPRQPRFSVSYLHYRS
                     LMNEFAAASTAFGEYFGLASGFFGASGSSQIGIQGGVFALFNLDAPSNDLINADYWIG
                     LPISYRKGPWSYLLRVYHQSSHLGDEFILGNPGIDRVNLSYEDMKFLVSYEWERWRLY
                     GGGGYILSSEPDLAPKHLQGGVEYVRPRAAGRLSFIAAADIQASEELDWRRSRSYQAG
                     FEFRSGSPRRARLMLEHFRGHSPNGQFYRESLRYTGLGLYFGF"
     gene            1630..>2437
                     /locus_tag="AC16FV_10555"
     CDS             1630..>2437
                     /locus_tag="AC16FV_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850532.1"
                     /translation="MDLKMKLDKKSLIQIVILIVLVAGGAGAYFMQQDGGLDFISGLF
                     ESKPTTVRAPASHAPAADKKPGAAPASAPATDKVAAKPVAPAIPAAPAKGQIHGKPFT
                     VENSYIESGMLTLRLGKDAAADLEVRVMLPGSPWETPAGKNFKVTEAGGAAVPQIVLA
                     WKEAGQSTPSEQKFTDKYSMVLEFGQEKDKKIPGKIQLNLPDETKSNVAGTFEADIRG
                     FRIVDGKPDLSADSVDTLQYLALRELLKDDPNKSLEVISFRNGRYAQPSSP"
BASE COUNT          486 a          704 c          730 g          517 t
ORIGIN      
        1 ttccacagca cgaaggtcag cggcggatcg ccaagcaggt cgagcagttt gcgcgccatc
       61 cagcgatcca gcaccgacac tcgcgatgag gcacggacca ggccggtctc gacccttccg
      121 ctcgtcaacg tcggactctt gagcgaagtc agcctggatt tgtgcacatc tttttccatg
      181 ggcccgctgc tccttcagga cggcttttat cctacctcgc cttcatcttc ctttacaaat
      241 gttgcacgct tgttgcacgc tgcacgcgct ttcaaacgcc ggtattcctt ggcatattca
      301 cgctatgtgt tgcagaggag tttacagaga gtacgacatg aggttcggcg cgacggcacg
      361 tgcaaggcgg gataatgtgt aaaatggcgg catgaaatac cggcggtatc catccatctt
      421 ctgtattctt gctggaattt cacgccagat tttcctgggc gcgttggtga tcggaataat
      481 ggcgccggtg tcggccgccg ggccggaaga atatctgcgt ggttatgtcg attcgctgct
      541 cgacagccgt ttccccggac ttggcctgcg ttcgcagatc atctcggcgg acggcgcagt
      601 gaaactgatg tcacgtacct gtctcggccc gtcccagaaa cgcgacgttt tgtatctgct
      661 gactgccacc ggccgggtga agaatgtcca gtgggatctg tccacggatt gtgacaaatc
      721 cgacgccgtg gagcaggaca aagcgggaac ggaacgcgag ccagagactc ccgttgatgt
      781 ttatgccttg cccgaacagg aattgttcgc accgctgctg gccgacccgc ggcagccgcg
      841 attttccgtg agctacctgc actatcgcag cctcatgaat gaatttgccg ccgccagcac
      901 cgccttcggc gaatatttcg gtctggcttc cggttttttc ggggcgtcgg gcagctccca
      961 gatcgggata cagggcggcg tgttcgcgct gttcaatctc gacgcgccgt cgaacgattt
     1021 gatcaacgcg gactactgga tcggccttcc gatcagttat cgcaagggcc cgtggtcgta
     1081 tctgttgcgc gtctatcatc agagttcgca cttgggcgat gagttcattc tgggcaatcc
     1141 gggcatcgac cgggttaatc tgagctacga ggacatgaag tttctcgtgt cttatgagtg
     1201 ggaacggtgg cgtctctacg gcgggggcgg ctatattttg agtagcgaac cggatctggc
     1261 gcccaagcat ctccagggcg gggtcgaata cgtccggccg cgcgcggccg ggagattgag
     1321 tttcatcgcc gcggcggaca tacaggcctc cgaggagctc gactggcgtc gcagtcgttc
     1381 gtaccaggcg gggttcgagt tccgcagtgg cagtccccgg cgggcgcgtc tcatgctaga
     1441 gcatttccgc ggccattcgc ccaatggcca gttttaccgc gaatccctgc gctacacggg
     1501 cctggggctt tactttggtt tctgaggaaa cccggcaaaa cggccggttc ggcgcgataa
     1561 aagcgattgg cggacgccgc tgggcatact attcttatgg cttaatggga aatcacagga
     1621 aggtggttca tggatctgaa aatgaagctc gataaaaaat ccctgattca aatcgtcatc
     1681 ctcatagtgc ttgtcgctgg aggcgccggg gcttatttca tgcagcagga tggcggtctg
     1741 gatttcatca gcggcctgtt cgagagcaaa ccgactactg tccgggcacc ggcctcccac
     1801 gcgcccgccg ccgacaaaaa gcctggcgca gcgccagcct ccgcgcccgc gactgacaag
     1861 gtcgccgcca aacccgtcgc tcccgcgatt cccgctgcgc ccgccaaggg acagattcat
     1921 ggcaaaccgt ttaccgtcga gaacagttac atcgagagcg gcatgctcac cctgcgattg
     1981 ggcaaggacg ccgccgccga tctcgaggtg agggtgatgt tgcccggctc cccgtgggaa
     2041 acgccagccg gaaaaaattt caaggtgacg gaagccggtg gcgccgctgt tccgcaaata
     2101 gtgctggcat ggaaggaggc cggccagagc acgccttcgg aacagaagtt caccgacaag
     2161 tacagcatgg tgctcgagtt cggtcaggag aaggacaaga aaattccggg caagatccag
     2221 ctcaatcttc ccgacgagac gaagagcaat gtggccggta cctttgaggc ggacatccgc
     2281 ggattccgca tcgtggacgg caagcccgat ctgtccgccg actcggtgga tacgctccag
     2341 tatctggcgc tgcgtgaact gctgaaggac gatccgaaca agtcacttga agtgatctcg
     2401 ttccgcaatg gacgctatgc ccagccatca tctccgg
//
LOCUS       DBNGZE010000411         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1029624, whole genome shotgun sequence.
ACCESSION   DBNGZE010000411 DBNGZE010000000
VERSION     DBNGZE010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1029624"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1993
                     /locus_tag="AC16FV_10560"
     CDS             <1..>1993
                     /locus_tag="AC16FV_10560"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR2850533.1"
                     /translation="KNLPQLTLPEMAMLAGLPKAPSRDNPLTNPDNALERRNAILKQM
                     LNLGFIDEAACTAAINTPLAAATQALKSDTAAPYVAELVRQYMIQTYDEKTYAGGFHV
                     YTTLNAKHQQAAVRALRAGLLDYEHRHGYRGPAGHVAIRGKPDVENLDDALKDYRLVG
                     DLLPGVVLKVEDKSAQVYTQDGATVELGWAGLSWARRYIDENTLGNAPRNAADVVKPG
                     DIIYLEAIEDPQQLQETGPWRLAQIPEVAGALVALRPSDGAVLALTGGFDFYQSSFNR
                     ATQAERQPGSSIKPFIFSAALDKGFTAASTVSGAPIVMEGESPEEEWRPEDYSKKFFG
                     PTRLRKALAQSLNLVAVRLLRAIGVSYTVEYMERFGFDTAKLPRNLSLALGTASATPM
                     QMVNAFAVFANGGYRVTPYFITRIEDANHNILEQAKPTVICRDCDVQGSTNAASAGRA
                     GAADVQGSTSVAKDKGVIFGSRMPGATDAQPNPSAAAITSAATDKDKPAYAPRVLSPE
                     NSFLMASMMQDVIRAGTGRGALVLGRRDLAGKTGTTNDYRDAWFSGFNSEVVATAWVG
                     FDQPVSLGRGETGGRTALPVWIDFMRVALEGVPEKPLVPPENIVRILINSESGKPTTP
                     DDPEAMEEYFVEGTETPRELPGIEGETTPETGPEKVPEN"
BASE COUNT          413 a          640 c          620 g          320 t
ORIGIN      
        1 ggaagaatct gccgcaactg acactgccgg aaatggcgat gcttgccggt ctgccgaagg
       61 cgccgtcgcg ggacaacccc ctgactaatc ccgacaacgc cctcgagcgg cgcaatgcca
      121 tcctgaaaca gatgctgaat ctcgggttca tcgatgaagc agcctgcacc gcggcgatca
      181 atacaccgct ggccgccgcc acgcaggcac tcaagtcaga caccgcggcg ccttacgtcg
      241 ccgaactggt gcgccagtac atgatccaga cctatgacga gaaaacctac gccggcggct
      301 ttcacgtcta caccacgctc aacgccaaac accagcaggc cgcggtcagg gcattgcgcg
      361 ccggtttgct ggattacgag caccgccacg gttaccgcgg gccggccggc cacgtggcga
      421 tccgcggaaa acccgacgtc gagaacctcg acgacgccct caaggattac cgcctcgtgg
      481 gcgatctgtt gccaggcgtg gtactcaagg tcgaggataa aagcgctcag gtctataccc
      541 aggacggcgc cacggtcgag cttggctggg ctggcctgtc ctgggcgcgg cgttacatcg
      601 atgaaaacac cctgggcaac gcccccagga atgccgccga cgtggtcaag ccgggcgaca
      661 tcatctacct cgaagccatc gaagatccgc aacagctcca ggaaaccggc ccctggcgtc
      721 tggcgcagat tccggaagtg gccggcgcac tcgttgcctt gcgtcccagc gacggcgccg
      781 tgctggcgct caccggcggt tttgattttt accaaagcag tttcaaccgc gcgacccagg
      841 cggagcgcca gcccggttcc agtatcaagc cttttatttt cagcgccgcg ctggacaagg
      901 gtttcaccgc cgccagcacg gtcagcggcg cgccgatcgt gatggaaggc gaatcccccg
      961 aggaggaatg gcgccccgag gactacagca aaaaattctt cggcccgacg cgtctgcgca
     1021 aggcattggc ccagtcgctc aatctggtgg ccgtgcgcct gctgcgcgcc atcggcgttt
     1081 cctataccgt cgaatacatg gagcgcttcg ggttcgacac cgcgaaactg ccgcgcaacc
     1141 tgtcgcttgc actcggcacg gcctcagcca cgccgatgca gatggtgaat gcctttgccg
     1201 tgttcgccaa tggtggttac cgcgttactc cgtacttcat tacgcgtatc gaggacgcca
     1261 atcacaacat cctcgaacag gcgaagccca cggtcatttg ccgtgactgt gatgtgcagg
     1321 gaagcacaaa tgccgcgagc gcaggacgcg caggagcggc cgatgtacag ggaagcacaa
     1381 gtgtcgctaa ggataaaggg gtgatcttcg ggtccaggat gccgggagcg actgatgcgc
     1441 agccgaatcc gtctgccgcc gcaataacga gtgccgctac cgacaaagac aagccggctt
     1501 acgcaccgcg cgtgctgagc cccgagaaca gctttctcat ggccagcatg atgcaggatg
     1561 tcattcgcgc cggtaccggc cggggcgcgc tggtgctggg gcgcagggac ctggccggca
     1621 agaccggaac caccaacgat tatcgcgacg cctggttctc gggcttcaac agcgaggtgg
     1681 tggcgacggc ttgggtcggc ttcgaccagc ccgtgtcgct cggccgcggc gaaactggcg
     1741 ggcgcacggc gctgccggtg tggatcgact tcatgcgcgt ggcgctcgaa ggcgttccgg
     1801 aaaagccgct ggtgccgccg gagaacatcg tgcgcattct tatcaacagc gaaagcggaa
     1861 agcccaccac gcccgacgac cccgaggcca tggaagaata tttcgtcgag ggcacggaaa
     1921 cgccgcggga acttcccggc atcgagggtg aaacaacccc ggagactggg ccggaaaagg
     1981 tgccggaaaa cag
//
LOCUS       DBNGZE010000412         5023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_613146, whole genome shotgun sequence.
ACCESSION   DBNGZE010000412 DBNGZE010000000
VERSION     DBNGZE010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_613146"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC16FV_10565"
     CDS             complement(<1..447)
                     /locus_tag="AC16FV_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HIR2850534.1"
                     /translation="MADNNHTGRGDGKGEKLLYCSFCGKSQHEVRKLIAGPSVFVCDE
                     CVELCNDIIREEVQEEKNAANAKKKFPTPREIHQILDEYVIGQSDAKKVLSVAVYNHY
                     KRIENEGKIGEVELSKSNILMIGPTGSGKTLLAETLARLLNVPFTIA"
     gene            complement(601..1230)
                     /gene="clpP"
                     /locus_tag="AC16FV_10570"
     CDS             complement(601..1230)
                     /gene="clpP"
                     /locus_tag="AC16FV_10570"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR2850535.1"
                     /translation="MKDTGQYGGALNPQALGLVPMVIETTGRGERAYDIYSRMLKERV
                     VFLVGQVEDQMANLIVAQLLFLESENPDKDIHLYINSPGGAVNAGLAIYDTMQFIKPD
                     VSTVCIGQAASMGALILTGGAAGKRFALPHSRMMVHQPMGGFQGQATDFDIHAREILR
                     VRERLNQIMVRHTGRTMDQIKEDTDRDYFMDGEEARGFGLIDAVIEKRT"
     gene            complement(1283..2572)
                     /gene="tig"
                     /locus_tag="AC16FV_10575"
     CDS             complement(1283..2572)
                     /gene="tig"
                     /locus_tag="AC16FV_10575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR2850536.1"
                     /translation="MQVSVETIGTLGRRLKVAVPADQVEKEFNARLQRLSKQVKMPGF
                     RPGKVPLKMVEAQYGGRLMDEVAGELIQTTLYEAIGSQGLRPAGDPRIQRKPVMRGEQ
                     LEYTVEFEIFPEVKRFDLTGSRIERPVVAVSEEDVNRTLETIRKQRATWKPVERAANT
                     GDRLTIDFVGRIDGKEFDGGKANGFQLVLGSGTLLEDMEQGLVGAKTGDMRSILVKFP
                     SDYRHAPLAGQTANFDVKVNEVTEAVLPELNEAFAQELGVKEGGLDKLRSDIRTNLER
                     EATGRARAVVRARALKLLLDANPVEVPRSLLDAEIQRLKTSDMSGGLNAADEATYEKR
                     ARSRVALGLILGEFIRSRGLVPDPARVRARLEEMAADYESPQEFIQWHYEKPERLSEI
                     ESLVMEEKVVEDLLVSAEIAEKPVSFQDLLKIETAVQ"
     gene            complement(2689..2773)
                     /locus_tag="AC16FV_10580"
     tRNA            complement(2689..2773)
                     /locus_tag="AC16FV_10580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2737..2739),aa:Leu,seq:tag)
     gene            complement(2913..2988)
                     /locus_tag="AC16FV_10585"
     tRNA            complement(2913..2988)
                     /locus_tag="AC16FV_10585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2953..2955),aa:Lys,seq:ctt)
     gene            complement(3001..3077)
                     /locus_tag="AC16FV_10590"
     tRNA            complement(3001..3077)
                     /locus_tag="AC16FV_10590"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3041..3043),aa:His,seq:gtg)
     gene            complement(3142..3218)
                     /locus_tag="AC16FV_10595"
     tRNA            complement(3142..3218)
                     /locus_tag="AC16FV_10595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3182..3184),aa:Arg,seq:tct)
     gene            complement(3272..3348)
                     /locus_tag="AC16FV_10600"
     tRNA            complement(3272..3348)
                     /locus_tag="AC16FV_10600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3312..3314),aa:Pro,seq:tgg)
     gene            3574..4434
                     /gene="folD"
                     /locus_tag="AC16FV_10605"
     CDS             3574..4434
                     /gene="folD"
                     /locus_tag="AC16FV_10605"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011705731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR2850537.1"
                     /translation="MSARILDGKKIAQELRAEIKQRVDRRLASGKRPPGLAVLKVGNE
                     AASEVYVRNKREACAAAGFISFDFDLPVDISQEKLLAHIDQLNTQPDVDGILVQLPLP
                     KHVDQTVIIERINPAKDVDGFHPYNLGRLAQRIPRLRPCTPYGVMRLLQTIGVALEGK
                     EAVVVGASNIVGRPMSLELLLAGCTVTTCHRYTQNLPGHVARADVLVVAVGKPGMVKG
                     DWIKPGAIVIDVGINRLPDGKLVGDVEFETAKQRAGWITPVPGGVGPMTVATLLLNTL
                     EAAEYRDNKE"
     gene            4472..>5023
                     /locus_tag="AC16FV_10610"
     CDS             4472..>5023
                     /locus_tag="AC16FV_10610"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase family protein"
                     /protein_id="HIR2850538.1"
                     /translation="MAPHSKNSPAGPASSLPLLHIDTTRLYRDGVGAEGTDKTEIEVL
                     VPELRRVHQAVVDKSAGGLDAELACLNLHHNMPESLPQIEAAAEQLRRFEDIAVIGIG
                     GSSLGAKAVYQALVDDPGARHPRLYFLENIDPRNLDLFVGNRSAENAAVVCISKSGGT
                     IETVIQYLIVREWLEQQLGKDKAR"
BASE COUNT         1065 a         1558 c         1353 g         1047 t
ORIGIN      
        1 ggcgatggtg aatggcacgt tgagcagccg cgccagggtc tcggccagca atgtcttgcc
       61 cgagccggtc gggccgatca tcaggatgtt gctcttggag agctcgacct cgccgatctt
      121 gccttcgttc tcgatgcgct tgtaatggtt gtagaccgcg accgacagca ccttcttggc
      181 gtcggactgg ccgatcacgt attcatcgag aatctgatgg atctcgcgcg gggtggggaa
      241 ttttttcttg gcgttggcgg cgtttttctc ttcctggacc tcttcgcgga tgatgtcgtt
      301 gcacaactcg acgcattcat cgcaaacgaa tacggaagga ccggcaatca gcttgcgcac
      361 ctcgtgctgg ctcttgccgc agaatgagca gtacaggagc ttttcgccct tgccatcgcc
      421 cctgcccgtg tggttgttgt cagccatcct taaacctcgc tatcgattaa ggatctttta
      481 tgccgcaaag gatgcgggtt tccgccaccc tttgcaagta ataatcgcaa gcaataatca
      541 ctccctttat caacattata ccttaaatct atagcatatc ctgcggaaag ttcacctgtt
      601 ttatgtgcgc ttttcgatga ccgcgtcaat caggccgaaa cccctggctt cctcgccgtc
      661 catgaaatag tcccggtcag tgtcctcctt gatctgatcc atggtgcggc cggtatgtct
      721 gaccatgatc tgattcaacc gttcgcgtac acgcagaatc tcgcgggcgt gtatgtcaaa
      781 gtcggttgcc tggccctgaa agccccccat gggctgatgc accatcatgc gggaatgcgg
      841 taatgcgaag cgtttgccgg cggcaccgcc ggtgagaata agcgccccca tgctggccgc
      901 ctgacctata cacaccgtgc tcacgtccgg cttgatgaac tgcatggtgt cgtaaatcgc
      961 caagcccgcg tttaccgcgc cacccggcga gttgatataa agatggatgt ccttgtccgg
     1021 gttttcggat tccaggaaca gaagctgggc cacgatcaga ttcgccatct gatcctcgac
     1081 ttgcccgacc aggaacacca cgcgctcctt gagcatgcgc gagtagatgt cataggcgcg
     1141 ctcgccacgt cccgtggttt cgataaccat gggaaccaaa cccaaggcct gaggattcag
     1201 cgcaccgcca tattgtccgg tatctttcat tatctcgttc cttccgttaa tcggcattct
     1261 ttatgagtcc atattaagca ctttactgaa cagcagtctc aatcttcaat aaatcctgga
     1321 aactcacggg tttttcggca atttcggcag aaaccagcaa gtcttccacc accttttcct
     1381 ccatgaccag gctttcgatt tcgctcagac gttccggctt ttcgtaatgc cactggatga
     1441 attcctgcgg tgactcgtaa tcggccgcca tttcctcaag ccgggcacgc actctggccg
     1501 gatcgggaac cagcccgcgg ctgcgaatga attcacccag aataagtccc aacgctactc
     1561 ggctgcgcgc ccgtttctca tatgtggcct cgtccgcggc attcaagccg cccgacatgt
     1621 cgctggtctt caggcgctga atctcggcgt cgagcaaact gcgcggcact tcgacaggat
     1681 tcgcatccag tagcagtttg agtgcgcgcg ccctcaccac ggcgcgagca cggccagtgg
     1741 cctcgcgctc aagattggtc ctgatgtccg agcgcaactt atcgagaccg ccttccttca
     1801 cacccaattc ctgggcaaat gcttcgttca gttccggcaa cactgcctcg gtcacctcgt
     1861 tgaccttcac gtcgaaattc gcggtctgtc ctgccagcgg cgcatgtctg tagtccgatg
     1921 gaaacttgac caagatgctg cgcatgtcac cggttttggc accgacaagg ccttgttcca
     1981 tatcttccag cagcgttcca ctgccaagca ccagctgaaa accattggcc ttgccaccat
     2041 cgaattcctt gccgtcgatg cgaccgacaa aatcgatggt aagccggtcg cccgtattgg
     2101 ccgcgcgttc aaccggtttc caggtggcgc gctgcttgcg aatggtctca agcgtgcgat
     2161 ttacgtcttc ttcgctcacc gccaccaccg gacgctctat gcgactgccc gtcagatcga
     2221 agcgttttac ttccggaaag atttcaaact cgacggtata ttcgagttgc tcaccgcgca
     2281 tgaccggctt gcgttgtatg cgcggatcgc cggccggacg caggccttgg cttccgatgg
     2341 cctcgtacaa cgtggtttga atcagttcgc cggctacttc atccatcagc cgaccgccat
     2401 actgggcctc gaccatcttg agcggcacct tgcccgggcg aaaaccgggc atcttcacct
     2461 gcttcgacag ccgctgaagc ctggcgttga attctttctc cacctgatcc gccggcaccg
     2521 cgactttcaa gcgcctcccg agcgtgccga tggtttcaac tgaaacttgc attggaaaac
     2581 cccataatca ataaacgata aaatattgaa ccggctgtat tttggagagg aatcataccg
     2641 atttgccgcg ggcaggccaa caaccacggt tttcgggcaa aaaagaggtg gtgcgaatgg
     2701 agggactcga acccccacgg gttaccccac tggaacctaa atccagcgcg tctaccagtt
     2761 ccgccacatt cgcagcaata accggacaag tatagcgcca agctgcttga ctccagccag
     2821 acaggcctca ccgcaagatc aggggacgaa agcaggcgga aacgccggga gggagacgtt
     2881 ctttccctct aaaacactca acggtgcaaa aatggtgggc cgtgtagggg ttgaacctac
     2941 gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgg gtcaaaaaac
     3001 tggggtgagc gatgggactc gaacccacga caaccggaat cacaatccgg gactctaacc
     3061 aactgagcta cgctcaccat aaagtgatca acgtcgccgg atattatacc cgcgaagaat
     3121 ctgcctgctc taccctgaaa ttggtgcgcc cggcaggagt cgaacctgcg accaccagct
     3181 tagaaggctg gtgctctatc caactgagct acgggcgcca gagaaacgcc cgcacattac
     3241 atcccaagca tcacccacgc gcaattgaaa atggtcgggg tagagggatt tgaacccacg
     3301 acaccctgct cccaaagcag gtgcgctacc aggctgcgct ataccccgat cggtactcac
     3361 gccaagcgcc gcttccgcga acactcgcaa aatcggcatg gtccggtcgg cgcgaggccg
     3421 gaactatacg caccgcttcc aggaccgtca atctggcgtg cctgaattat aggcaaaaat
     3481 caggtaaaag tgcgatggcc tcgcttggcg cgccgccgtg gtgcgtgcga tgatgcgccg
     3541 ggcgcattcc tccgcgctgc agctgaagaa accatgagcg cacgcatcct cgacggaaag
     3601 aaaatcgcac aagaactgcg cgctgagatc aaacagcggg ttgaccgccg tctggcgtcg
     3661 ggcaaacggc cgcccgggct cgccgtgctc aaggtcggca acgaagccgc ctccgaggtc
     3721 tacgtgcgca acaagcgcga ggcctgtgcc gcagccggtt tcatttcctt cgatttcgac
     3781 ctgcctgtcg acatttccca ggaaaaactc ctggcccaca tcgaccagct gaacacgcag
     3841 ccggatgtgg acggcatcct ggtgcagctc cccctgccca aacacgttga tcagaccgtc
     3901 attatcgaac gcatcaatcc cgccaaggac gtggacgggt tccatcccta caacctcggc
     3961 cggctggcgc agcgcatccc gcgcctgcgt ccgtgcacgc cctacggcgt catgcgcctg
     4021 ctccagacca taggcgtagc gctggaaggc aaggaggctg tcgtggtcgg cgcctccaat
     4081 atcgtcggac gccccatgtc gctcgaactg ctgctggccg gctgcaccgt cacgacttgc
     4141 catcgctata cccaaaatct gcctgggcat gttgcccgtg ccgacgtgct ggtggtcgcg
     4201 gtcggcaagc ccggcatggt caagggcgac tggatcaagc ccggtgcaat tgtcatcgac
     4261 gtcggcatca accgcctgcc tgacggaaaa ctggtcggcg acgtggaatt cgaaaccgcc
     4321 aaacagcgcg ccggatggat cacccccgtg ccgggcggtg tcgggccgat gaccgtggcc
     4381 acgctgctgc tgaacacgct ggaggccgcg gagtatcgcg acaacaaaga atgatagtat
     4441 ggcgtcagcc ctgatgcaga tgagatagcc cgtggcgccc cacagcaaaa attcacccgc
     4501 cggtcccgcc tcttcattac cgctgttgca cattgacacc acgcgcctgt atcgcgacgg
     4561 cgtcggcgcc gaaggcaccg acaaaaccga gatcgaggtg ctggtgccgg aactgcggcg
     4621 cgtgcatcag gcggtcgtcg ataaatccgc gggtgggctg gatgccgagc tcgcctgcct
     4681 caacctgcac cacaacatgc cggaatcgct gccgcaaatc gaagccgcgg ccgagcagct
     4741 gcgtcgtttc gaggacatcg ccgtcatcgg catcggcggt tccagtctcg gcgccaaggc
     4801 cgtgtatcag gcgctcgtgg atgaccccgg cgcgcgacat cctcgtctgt atttcctcga
     4861 aaacattgat ccgcgtaatc tggatttatt tgtaggaaat cgttccgctg aaaatgcagc
     4921 agtcgtctgc atcagcaaat cgggcggcac gattgaaacg gtcatccagt atttgatcgt
     4981 gcgcgaatgg ctggaacaac aactgggcaa ggacaaggcg cgc
//
LOCUS       DBNGZE010000413         2005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_751626, whole genome shotgun sequence.
ACCESSION   DBNGZE010000413 DBNGZE010000000
VERSION     DBNGZE010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_751626"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16FV_10615"
     CDS             <1..759
                     /locus_tag="AC16FV_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2850539.1"
                     /translation="VVSISHVSKHFDSVIAVDDISFDVTARSTTALLGGNGAGKTTTL
                     SMLLGLLLPTAGRISVFGVDMLRDRHRVLPRLNFSSPYVDLPRRLTVRENLTVYARLY
                     GIIDIPARIAALGEELQIGRFLKRPYGQLSAGEKTRVVLAKALLNEPELLLLDEPTAS
                     LDPDTADYVRGMLEAYRQRTGTTMLLASHNMSEVERLCDDVIMLRAGKLVDRGTPAQL
                     LTRYGRRNMEEVFIDIARERRLEIALGDKQAAKP"
     gene            756..1583
                     /locus_tag="AC16FV_10620"
     CDS             756..1583
                     /locus_tag="AC16FV_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2850540.1"
                     /translation="MSVIAREHTGFVPSLRRVYALVLRHTYLLRSSGPRILELVYWPT
                     VQMILWGFITVFLVNHSSWIAQASGVLLSGVLLWDVLFRGQLGVSLVFMEEMWSRNLG
                     HLFVSPLRPFELGCALITMSLIRTLIGVGGAALIAIPLFHYSIFSMGLPLLAFFINLI
                     VMGWAIGLLVSGIVLRYGLGAESMAWIAIFAVQPISGVYYPIETLPVWLQYVAAILPS
                     SHVFEGMRAVLFDHSFRVDLLLHAVLLNVVYLAAGFVSFLAFFKVARVRGQLLHVGE"
     gene            1753..1980
                     /locus_tag="AC16FV_10625"
     CDS             1753..1980
                     /locus_tag="AC16FV_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850541.1"
                     /translation="MPYFVFRVSANRELKLVKTCEKYKEAKDLCRELRLSESPDNPNT
                     IRMAFAENEIKAKHLLAEKHQPSSPLEEWEA"
BASE COUNT          343 a          587 c          606 g          469 t
ORIGIN      
        1 gttgtttcca tttcccacgt cagcaagcat ttcgacagcg tcatcgctgt cgatgacatc
       61 agcttcgatg tcacggcgcg gtccaccacc gcgctgctcg gcggcaacgg cgccggcaag
      121 actacgacgc tttccatgtt gctcggtctg ctgctgccga ccgcgggccg catttccgtg
      181 ttcggcgtgg acatgctgcg cgaccgtcat cgcgtcttgc cgcgtctcaa cttctcttcg
      241 ccctacgtgg acctgccgcg ccggctgacg gtacgcgaaa acctcacggt ctacgcgcgt
      301 ctttacggga ttatcgacat ccccgcgcgc attgccgcgc tcggcgagga actccagatc
      361 gggcgtttcc tgaagcgccc ctacgggcag ttgtcggcgg gggagaagac ccgtgtggtg
      421 ctggccaagg ccctgctgaa tgaaccggaa ttattgctgc ttgatgagcc gaccgcctcg
      481 ctcgaccctg atactgccga ctatgtgcgt ggcatgctcg aagcctatcg ccagcgcacc
      541 ggcaccacga tgttgctggc ctcgcacaac atgtcggagg tggagcggtt gtgtgacgac
      601 gtcatcatgc tgcgcgccgg caagctggtg gaccgtggca cgccggcgca attgctgacg
      661 cgttacgggc ggcgcaacat ggaggaggtg ttcatcgaca tcgcgcgcga gcggcgcctg
      721 gaaatagccc tcggcgacaa gcaggcggcg aagccatgag cgtgattgcg cgcgaacata
      781 ccggttttgt tccctccctg cgccgggttt acgcgctggt gctgcggcat acctatttgc
      841 tgcgcagctc cgggccgcgc attctggaac tggtgtactg gccgacggtg cagatgatcc
      901 tgtggggatt catcacggta ttcctggtca atcacagttc gtggatcgcg caggcctcgg
      961 gggtgctact ctccggcgtg ctgctgtggg acgtgctgtt ccgcggccaa ctcggggtgt
     1021 cgctggtgtt catggaagag atgtggtcgc gcaatctggg acatttgttc gtcagtccgt
     1081 tgcggccctt cgagctgggc tgtgcgctga ttaccatgag cctgatccgc acgctgatcg
     1141 gcgtcggcgg cgcggcgctc atcgcgattc cactgtttca ttattcgatt ttcagcatgg
     1201 gcctgccgtt gctggcgttt ttcatcaacc tcatcgtcat gggctgggcc atcgggctgc
     1261 tggtctcggg tatcgtgctg cgctacggtc tcggcgccga gagcatggcg tggatcgcca
     1321 tcttcgcggt gcagcccatc agcggcgtgt attacccgat cgagaccctg ccggtatggc
     1381 tgcaatacgt cgcggccatc ctgccgtcga gccatgtgtt cgagggcatg cgagcggtgc
     1441 tgtttgacca tagcttccgc gtggatttgc tgctgcacgc ggtgctgctc aacgtggttt
     1501 acctcgccgc cggttttgtt tcgttcctgg cgttcttcaa ggtcgcgcgg gtgcgcggcc
     1561 agttgctgca cgtcggcgag taatgacacg ggaatcgcgc cggggtagca ttccggggct
     1621 tttattatta gagttctaag gaaatgaatg gacaggattc acaggattaa caagattttt
     1681 atttcacaag atttttaatc ctgtaaatcc tgtctatttt tattttttat ttcttaacca
     1741 caggaacaca ccatgcctta tttcgttttc cgggtctcgg ccaacaggga gctgaaactc
     1801 gtcaaaacct gcgagaaata caaggaagcg aaagatctct gccgcgagtt gcggctttcc
     1861 gaatcgcccg acaatcccaa caccatccgc atggcctttg ccgagaacga gatcaaggcc
     1921 aagcatttgc tcgccgaaaa gcatcaaccc agctcgccgc tggaggaatg ggaggcctga
     1981 tttcgccgcc gaacggttat cgtcc
//
LOCUS       DBNGZE010000414         2628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_404842, whole genome shotgun sequence.
ACCESSION   DBNGZE010000414 DBNGZE010000000
VERSION     DBNGZE010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_404842"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1193
                     /locus_tag="AC16FV_10630"
     CDS             195..1193
                     /locus_tag="AC16FV_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR2850542.1"
                     /translation="MRFLKQVVMLLIVTLVSPVMAADEIVIYSARNEQLIKPMFDAYT
                     AETGTQIKFITDKEGPLLERLKAEGKNTPADMLITVDAGNLWLAAKEGVLAPVNSKVL
                     KANIPAHLRDPSNQWFGLSVRARTIVYSTTRVKPADLSTYEALGDARWKGRLCLRTSK
                     KVYNQSLVAMMIARHGEAKTEEIVKSWVANLATDVFPDDTKLMEAIAAGQCDVGIVNT
                     YYFGRLKQKQPEVAIALFWPNQKDRGVHVNVSGAGITKHAKHRAAAVKFLEWLSSDKA
                     QNLFADENMEYPANPKNKPHASVAAWGTFKPDTINVSKAGSLQAKAVMLMDRAGYK"
     gene            1217..1414
                     /locus_tag="AC16FV_10635"
     CDS             1217..1414
                     /locus_tag="AC16FV_10635"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HIR2850543.1"
                     /translation="MYVCLCKGVTDCQIREAVCEGACSMRELGTCLGVATQCGRCARH
                     ARQVLEETLKTASCAENDKAA"
     gene            1476..1943
                     /gene="bfr"
                     /locus_tag="AC16FV_10640"
     CDS             1476..1943
                     /gene="bfr"
                     /locus_tag="AC16FV_10640"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR2850544.1"
                     /translation="MKGDAKIIEQLNKLIAGELTAIDQYFIHSRMYENWGLKKLYERI
                     EHERTEEVGHAAMLIQRVLFLEGTPNVAARDPLKIGKDVPGMLKNDLDLELSVIGALK
                     GAIAACESAHDYQTREILEKLLEDTEEDHAHWLEQQLWLIEKTGLQNYLQSQM"
     gene            1953..2417
                     /gene="bfr"
                     /locus_tag="AC16FV_10645"
     CDS             1953..2417
                     /gene="bfr"
                     /locus_tag="AC16FV_10645"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR2850545.1"
                     /translation="MKGDAKIIEQLNQALVHALTAINQFFLHARMYKNWGLGKLNERV
                     YKESIGEMKRADKLIERILFLEGLPNLQDLGKLLIGEHAGEMLKLDLQLETDARVQLQ
                     DAIARCEHAGDYVSRDLLEDILEEREEHIDWLETQLDLIGKTGVQNYLQSQM"
     gene            2430..>2628
                     /gene="hemP"
                     /locus_tag="AC16FV_10650"
     CDS             2430..>2628
                     /gene="hemP"
                     /locus_tag="AC16FV_10650"
                     /inference="COORDINATES: protein motif:HMM:NF022103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HIR2850546.1"
                     /translation="MSSRRSNDIPVKRGEAGNVPPDHGTACSAARPADSGSVPRVHSC
                     ELLRNGAQLVIEHAGREYRLRV"
BASE COUNT          604 a          726 c          829 g          469 t
ORIGIN      
        1 ctcgcgtggc gcatgctgac caaaacccgc gccgccggct ggcgtcacgg actggcgttt
       61 ggagccttgc tcgttggcat ggcgctgccc gccggcatgt ggttcgttca attctttatg
      121 tggtgacgct gaaagacaga cggcgatata tttactgatc attaaatttt tttcattttt
      181 tgaaggagaa tgacatgcgt ttcctgaaac aggtcgtgat gttgctgatc gtaacgctcg
      241 tgtctcccgt tatggcggcg gatgagatcg tgatttattc cgcgcgcaac gagcagctga
      301 tcaagccgat gttcgacgcc tataccgccg agaccggcac gcagatcaaa ttcatcaccg
      361 acaaggaagg gccgttgctg gaacggctca aggccgaggg caagaacacg ccggcggaca
      421 tgctgattac ggtggacgcg ggcaatctgt ggctcgcggc caaggaaggc gtgcttgccc
      481 cggtgaattc gaaagtgctc aaggccaata tcccggcgca tctgcgcgat ccgagcaacc
      541 agtggttcgg tctgtcggta cgcgcgcgca ccatcgtcta cagcacgaca cgcgtgaagc
      601 ccgcggatct ttcgacctac gaggcgctgg gcgatgccag gtggaaggga cggctgtgcc
      661 tgcgcacctc gaaaaaggtc tataaccagt cgctggtggc gatgatgatc gcgcgccacg
      721 gcgaggccaa gacggaagag atcgtgaaat cctgggtcgc gaacctggcg accgatgttt
      781 tcccggacga caccaagctg atggaggcga tcgccgccgg ccagtgcgac gtcggcatcg
      841 tcaacaccta ctattttggc cgtctcaaac agaaacaacc cgaggtggcg atcgcgctgt
      901 tctggcccaa ccagaaggat cgcggcgtgc acgtcaatgt ctcgggcgcg ggaattacca
      961 agcatgcgaa acaccgcgcg gcggcggtga agtttctcga atggctgtcc tcggacaagg
     1021 cgcagaacct gttcgcggac gagaatatgg aatatccggc caatccgaag aacaagccgc
     1081 acgcgagcgt tgcggcctgg ggcacgttca agccggacac catcaacgtc tcgaaggccg
     1141 gcagcctgca agccaaggcc gtcatgctga tggaccgggc gggatacaag taacgcacgc
     1201 gaaagagggc tggaatatgt acgtatgttt gtgcaaaggc gtcaccgact gtcagatacg
     1261 cgaggcggta tgcgagggcg catgctcgat gcgtgaactc gggacgtgcc tgggcgttgc
     1321 cacccaatgc ggccgatgcg cgcggcacgc gcggcaggtg ctcgaggaaa cgttgaaaac
     1381 cgcgtcgtgc gcggaaaatg acaaagccgc ttgagggcca tccagcggcg gcgcatgctg
     1441 gagaagaata aatcaggcac aaacctggag gatatatgaa aggcgacgca aagattatcg
     1501 aacagttgaa caagctgatc gcgggcgaac tgacggcgat cgaccagtat ttcatccatt
     1561 cacggatgta cgagaactgg ggactcaaga agttgtacga acgcatcgaa cacgagcgca
     1621 ccgaggaggt cggccacgcg gccatgctga tccagcgcgt gctgtttctc gaggggacgc
     1681 ccaacgtcgc cgcgcgcgat ccgctgaaga tcggcaagga cgtgccggga atgctgaaga
     1741 atgacctcga tctcgagctt tccgtcatcg gcgcgctcaa gggcgcgatt gcggcctgtg
     1801 agtcggccca tgactatcaa acccgcgaga tcctggagaa gttgctcgag gataccgaag
     1861 aggatcacgc ccattggctg gagcagcagc tgtggctgat cgagaaaacg ggattgcaga
     1921 attacctgca atcgcagatg tgaggccgcg ccatgaaggg tgatgcaaag atcatcgaac
     1981 aactgaacca ggcgctggtc cacgcgctca cggccatcaa ccagttcttc ctgcacgcgc
     2041 gcatgtacaa gaactggggc ctcggcaagc tcaacgagcg cgtgtacaag gaatcgatcg
     2101 gcgagatgaa gcgcgcggac aagcttatcg agcgcatcct gtttctcgaa gggctgccga
     2161 atttgcagga cctcgggaag ctgctgatcg gtgaacatgc gggcgagatg ctcaaactcg
     2221 acttgcaact cgaaacagac gcgcgcgtcc agcttcagga cgccatcgcc cgctgcgagc
     2281 atgccggtga ttatgtttcg cgcgatctgt tggaagacat cctcgaggaa cgggaggagc
     2341 acatcgactg gctcgaaacc cagctcgatc tgatcggaaa gaccggcgtg cagaattatt
     2401 tgcagtcaca aatgtagaaa tgaagagaca tgtcgtcacg ccgtagcaat gatattcccg
     2461 tgaagcgggg agaggcagga aacgttccgc cggatcacgg gaccgcctgt tcggccgcac
     2521 gcccggctga cagcggctcc gtgccgcgcg ttcacagctg cgagctgttg cgcaacggcg
     2581 cgcaattggt catcgagcac gccgggcgcg aataccgcct gcgtgtga
//
LOCUS       DBNGZE010000415         4214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1790875, whole genome shotgun sequence.
ACCESSION   DBNGZE010000415 DBNGZE010000000
VERSION     DBNGZE010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1790875"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="AC16FV_10655"
     CDS             <1..930
                     /locus_tag="AC16FV_10655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850547.1"
                     /translation="DVVLDIGSNDATTLKAYQAPQIQRLGIDPTGKKFASFYTGDIRL
                     VPDFFSGRAYRSVENRPARIVTSIAMFYDLDNPIDFAKEIASVMADDGIWHFEQSYMP
                     SMLRTNSYDTICHEHLEYYSLGVVCRILDAADLKPIDVAMNAVNGGSFAVTAAKRSNR
                     SIHSNRAVIDWLLEQEDRMGLGTPRPFRSFEERVYRHRDDLKRLLDSLNADGKKILGY
                     GASTKGNVILQFCGLGPKDIPAIADVNPDKFGAYTPGSLIPIISESDAKAMKPDYFLV
                     LPWHFKDGIIRREKAFLENGGNFIFPFPEIEIV"
     gene            1087..2619
                     /locus_tag="AC16FV_10660"
     CDS             1087..2619
                     /locus_tag="AC16FV_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR2850548.1"
                     /translation="MSRLRHNVIANIVGQGWAVLMTVLVVPLYIKLVGIEAFGLIGFF
                     VMLQGLLVVLDLGLGQTLNREMAKYSALPDTLKETRNLLRSLEIVYWIVGIVMGASIV
                     MLSPVIAQHWIKAQALSQETVQRAVLMMGLIVALQWPVSLYLSGLMGLQRQVLVNAIR
                     IATSTVAGVGGVAVLGLLSRDIVALFIWQVVVAGVSVVLVAVLLWRALSLHGHSPRFS
                     SAALQRVKRFAAGMTGITLTGIVLIQADRVVLSNLLSLDRFAYYTLAWVVANGMYVFI
                     GPIYSSFSPRFAALAVHGNTAELRSLYHQGCQLMAALIAPVAAVLFFCSHEVIELWTG
                     AAETAANAAPIVSLLIIGTTLNGLMNLPFALQLAHGRTGIGLAINLALIALAFPTLIF
                     MANTFGAVGGAAVWAGLNALYLLVGIPVTHHKLMPGATREWLLRDVALPFIVAVAIVV
                     ILRSILGASAGQVETILHLFLISMSALVASMWATPITRQHTLQVLRRGRKWVRRHATL
                     QA"
     gene            2784..3497
                     /locus_tag="AC16FV_10665"
     CDS             2784..3497
                     /locus_tag="AC16FV_10665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850549.1"
                     /translation="MTVEVLKSLEDVDVARKKLHDMGLSCLGGPVARLARRFGLWPSI
                     GVGDEIKSWDVLRTMEFIMANAARDSSVLDIGAYASEIPCLLHKAGYRRIAAIDLNPD
                     IRRMPYNNSIRYEVGNFMATPYPDRSFDVVTAISVIEHGFDGSRLLRELSRLLRPGGY
                     FIASFDYWPEKIDTTGVKFFGMDWLIFSKEDVAVFAEQAENYGLIPFGEMKYVAKDKA
                     IDCGGKQYTFAWMVLRKKF"
     gene            3500..>4214
                     /locus_tag="AC16FV_10670"
     CDS             3500..>4214
                     /locus_tag="AC16FV_10670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850550.1"
                     /translation="MKILLVTGSFPPGKCGIGDYSYRLAKVLAAQPDMHVGVLTSASF
                     GDGQMCEGIEMFPVVHGWRLREIQKFVKLIRSWSPDIVHVQYPTQGYAGGLLSWFLPL
                     VAFVLGKKVVQTWHEFYRNFRIALPFFTKAVVPGRLVVVRPEYKQGLHPLFRLALSKR
                     KFLYIRSASSIPRIHLGNREKNELRARYLNGQKRLIVFFGFVYPNKGVELLFEIANPA
                     SDHIVIAGETGEGGEYRKQI"
BASE COUNT          870 a         1036 c         1234 g         1074 t
ORIGIN      
        1 gatgttgtcc tagacatcgg cagtaacgat gcgactacac tgaaggcgta ccaggcgcca
       61 cagattcaac gcctgggaat cgatccaacc ggcaagaaat tcgcaagctt ctacaccggt
      121 gatatcagac tggtgcctga tttcttctca ggccgggcct atcgttccgt tgaaaaccgc
      181 cctgcgcgca tcgtgacttc gatcgccatg ttttacgacc tggataaccc gattgatttt
      241 gccaaagaga ttgcctccgt gatggcggac gacggtatct ggcattttga acagagctat
      301 atgccgtcca tgttgcgaac caactcatac gacacgatat gccacgaaca cctcgagtat
      361 tactcgcttg gcgtggtgtg ccgcattctc gacgcggctg acctgaagcc tatcgacgta
      421 gctatgaatg cggttaatgg cggcagtttc gctgtcacgg ccgcaaagcg ctccaaccgc
      481 agcatccact ccaaccgggc ggtcatcgac tggttgctgg agcaggaaga tcgcatgggg
      541 cttggcacgc cgcgcccatt caggagtttt gaggaacgag tataccggca tcgtgatgat
      601 ctcaagcgcc ttctcgacag cttgaatgcg gatggcaaga agattctcgg ctacggagcc
      661 tccaccaagg gtaacgtcat actgcaattt tgcgggcttg gtccaaaaga cattccggcg
      721 atcgcggacg tcaatcccga taaattcggc gcctacactc cgggttcgtt gatcccgatc
      781 atttccgaga gcgatgccaa ggccatgaag ccagattact ttcttgtact gccctggcac
      841 ttcaaggatg gcatcatccg gcgcgaaaag gcatttcttg agaacggcgg gaattttatt
      901 tttccgtttc cggagatcga gatcgtctga ttcttaccgt cagttttgcg atgtcatcca
      961 cattgcgcag tatcgctgac cctcaatgat gttttcttgg ccgtacgatc acgcaccgca
     1021 ggcccgaatg atattcaggt gcgttggttg tccatatgtg gatgctgata tgcggataac
     1081 cgaaagatga gccgtttacg gcataacgtc atcgccaaca tcgtcggcca aggctgggcg
     1141 gtgttaatga ccgtgctggt cgtgccgctg tatatcaaac tggtcggtat tgaagctttt
     1201 gggcttatcg gtttctttgt aatgttacag ggcctgttgg ttgtcctgga ccttggcctg
     1261 gggcagactt tgaatcgtga aatggctaaa tattcggccc tgcctgatac cttgaaggaa
     1321 acgcgaaacc tcctgcgttc actcgagatt gtgtactgga ttgttggaat cgtcatgggc
     1381 gccagcatcg tcatgctctc gccggtcatc gcacagcact ggatcaaggc gcaggcgctt
     1441 tcacaggaaa cggtgcagcg agcggtgctg atgatgggtc tgatcgtggc cctgcagtgg
     1501 ccggtcagtc tctatttgag cggattgatg gggctccagc gccaggtgct ggtcaacgcg
     1561 attcgtatcg ccacgtcgac cgtggcaggc gttggtggcg ttgccgttct gggcctgctg
     1621 tcgcgtgaca tcgtcgcgct ctttatctgg caagtcgtcg tggccggcgt aagtgtagtg
     1681 ttggttgcgg ttctactctg gcgagctctc tctttgcacg ggcattcgcc gcgtttcagc
     1741 agcgcagcgt tacaacgcgt gaaacgcttc gccgccggca tgacgggcat caccctaacc
     1801 ggtattgttc tgatccaggc agacagggtg gtgctgagca acctgcttag cctggaccgc
     1861 tttgcgtatt ataccttggc atgggtggtg gccaacggca tgtacgtatt tattgggccg
     1921 atctatagca gtttctcccc acgctttgcg gctttggcag tccatggcaa tactgcggaa
     1981 ctgcgttcgt tgtaccatca gggctgtcag ttgatggccg cactaatcgc gccggttgcg
     2041 gcggtcttgt ttttctgctc ccacgaagtc attgagctct ggaccggtgc tgccgaaacg
     2101 gcggctaacg ccgcaccgat tgtgagcctg ttgatcattg gtacgacgct caatggtctg
     2161 atgaatctgc catttgcgtt gcagctggcg cacgggcgta ctggtatcgg cctggcgatc
     2221 aatctcgcat tgatcgcact ggcctttccg accctgatct tcatggcaaa caccttcggc
     2281 gctgtcggtg gtgccgcggt ctgggcgggg ctgaacgctc tctatctgct tgtcggaatt
     2341 cccgtgacgc atcacaaact gatgccaggc gctacccgcg agtggctatt gcgtgatgtt
     2401 gccttgccat tcatcgtggc ggtggcgata gtagtgatcc tgcggtcaat actcggcgcc
     2461 agcgcaggtc aggtggagac gatccttcac ctgttcctga tatccatgtc agcattggtt
     2521 gcatctatgt gggcaacacc gataacccgc cagcacacgc ttcaagtgtt acgccgaggg
     2581 cgaaaatggg tgaggcggca tgcaacattg caggcctagt cccgtgattt tcggtgtttc
     2641 gggattataa ggtgttgtgg ttttttgtgg cccaaagaag cctggacgca cacgaagaaa
     2701 ggaatagata tacgcatgca gttttgcgtg aatcatctcg attggcgata ttgtgctcat
     2761 gaatcaaact ggaaactgaa tatatgacgg tagaggtgct gaagtcgctg gaagatgtag
     2821 atgttgcccg gaagaagctg cacgacatgg ggctttcctg cctcggcggg cctgtcgcga
     2881 ggttggcgcg acggttcggg ctgtggccaa gcataggcgt tggcgatgaa atcaagagct
     2941 gggatgtcct gcgaaccatg gagttcatca tggcgaatgc ggcgcgggat tcgtctgttc
     3001 tcgatatcgg tgcttatgca tccgagatac cctgtttgct gcacaaggcc ggttaccgcc
     3061 gtatagcggc aatcgatctc aatcccgaca tccggcgtat gccttacaac aattcgattc
     3121 gttatgaagt ggggaacttc atggcgacgc cctatcctga ccggtcgttt gatgtcgtga
     3181 cggcgatctc ggtgatcgag catggttttg acggatcacg gttgctcagg gagttgtcgc
     3241 ggctgctgcg gcctggagga tatttcatcg cttcattcga ttactggccg gagaagatag
     3301 atacgactgg cgtcaagttc ttcggcatgg attggctcat cttttccaag gaagatgttg
     3361 ctgtgttcgc agagcaggct gaaaattatg gattgatacc gtttggcgag atgaagtatg
     3421 tcgccaaaga caaggcaatt gattgcggcg gtaagcagta tacgtttgcc tggatggttc
     3481 tccggaagaa gttctgaacg tgaagatatt gctggtaaca gggtctttcc ctcccggaaa
     3541 gtgcgggatt ggtgactaca gctacagact cgcaaaggtc cttgcagcgc aaccagatat
     3601 gcatgtcggg gttctgacaa gcgcttcgtt tggagacggt cagatgtgcg aggggattga
     3661 gatgttcccc gtagtgcacg gttggcgcct gagggaaatc cagaagttcg tcaagcttat
     3721 acggagctgg tcgccggaca ttgtccacgt ccaatatccg acccaaggtt atgcgggtgg
     3781 cctgttgtca tggtttttgc cgttagtggc cttcgtgctg ggaaaaaagg ttgttcagac
     3841 atggcacgag ttttacagga attttcgcat tgcgctgcct ttttttacaa aggcggtagt
     3901 gccgggcagg ttggtggttg tgcgtccgga gtataagcag ggcctgcatc cgttattcag
     3961 gctggccctg tcgaaaagaa agtttctgta catacgaagc gcgtcgtcca ttccgcgaat
     4021 tcatcttggc aaccgggaaa aaaatgagct aagagctcgt tatttaaatg gacagaaacg
     4081 cctgatcgtt tttttcgggt ttgtgtatcc aaataaaggg gttgagttgc tgttcgagat
     4141 tgccaatcct gcgtctgatc atattgtaat agccggagag acaggggagg gcggcgagta
     4201 tcggaaacag atat
//
LOCUS       DBNGZE010000416         3015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1030580, whole genome shotgun sequence.
ACCESSION   DBNGZE010000416 DBNGZE010000000
VERSION     DBNGZE010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1030580"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16FV_10675"
     CDS             complement(<1..644)
                     /locus_tag="AC16FV_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR2850551.1"
                     /translation="MSNTSAEKQSFLPEFGTFSMLLQFVALAELIAIIITIGRNAELN
                     EQAIQDFSLLSVFAVAIALCSIIVLKIISPLLRRSSTGTGSVLVITMLLLVTSLGIDG
                     IIYLLHYLAIIEDRWPAWRETLLIRSLMITTIIGTLGVRYLILRERADTHSRLQQESK
                     LQALQSRIRPHFLFNSLNSVASLTRSDPSKAEAVLHDLADLFRVLLADARKLVPV"
     gene            1112..2500
                     /gene="argH"
                     /locus_tag="AC16FV_10680"
     CDS             1112..2500
                     /gene="argH"
                     /locus_tag="AC16FV_10680"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR2850552.1"
                     /translation="MAKQKPWSGRFTEPTDAVVEAFTASVDFDRRLYVYDIMGSIAHA
                     QMLAKVGVLTKKECDAIVRGLKDIEAEIDAGKFDWSVALEDVHMNFEARLIQRIGETG
                     KKLHTGRSRNDQVATDLRLYLRDGIDVVAHALGQLQQALLDLAEREAGTPMPGFTHLQ
                     VAQPITFGHHLLAWFEMLARDAERLRDCRRRLNVMPLGAAALAGTSFPIDRAYTTKLL
                     GFDAPAENSLDAVSDRDFAIEFVAACSLLMTHLSRMSEELVLWSSSQFGFVELSDAYC
                     TGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLTLMKAQPLAYNKDNQEDKEPVFDTL
                     DTVLGSLRVFAGMIPSMKVRRDHLLRAAVMGHATATDLADYLVRQGVPFRDAHEIVGK
                     AVRLAIDTDRDLSQIDLDEYKKLSPAIGKDVYGVLTVEGSLKARNHLGGTAPAQVKAA
                     IKRARKRITKKR"
     gene            2633..2971
                     /locus_tag="AC16FV_10685"
     CDS             2633..2971
                     /locus_tag="AC16FV_10685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIR2850553.1"
                     /translation="MDTLEDLRKRLREFARERDWEQFHSPKNLSMALIVEAAELVEHF
                     QWLTEAESRALAPEKREPVAQELADVFIYLNRMADLLGIDLMDAARRKIELNAKKYPA
                     HEVRGKADKR"
BASE COUNT          653 a          882 c          933 g          547 t
ORIGIN      
        1 accggcacga gcttgcgcgc atccgccagc aacacgcgga acaggtcggc gaggtcgtgc
       61 agcaccgcct cggccttgga gggatcgctg cgcgtgagcg aggcgacact attgaggctg
      121 ttgaacagga aatgcgggcg gatgcgcgac tgcaatgcct gcaatttgga ctcctgttgc
      181 agccggctgt gggtatccgc gcgctcgcgc agaatgaggt agcgcacgcc cagcgtgccg
      241 atgatggtgg tgatcatgag gctgcggatc aggagcgttt cgcgccacgc cggccagcgg
      301 tcttcgatga tggcgaggta atgcagcaga tagatgatgc cgtcgatgcc gagcgaggtg
      361 acgagcagca gcatggtgat caccagcacc gagccggtgc cggtcgagct gcgccggagc
      421 aggggcgaga tgattttaag cacgatgatg ctgcacagcg cgattgccac cgcgaacacg
      481 gacagcagcg agaaatcctg tatcgcctgt tcgttcaatt cggcgttgcg tccgatggtg
      541 atgatgatgg cgatcagttc ggccagggcg acgaactgca acagcatgct gaaggtcccg
      601 aattcgggaa ggaacgattg tttctcggcg cttgtgttgg acatggaatc cgttcgggcg
      661 tacgactttc cggcatgctt ccgtgccgga tctatgttgc ttttgggcct ggtagccggt
      721 gcacgacggg gctaccgcgc cccaccgatt gaatttatag cttaattata taagttaccg
      781 cggacggatg gggtgcgggc gggggaaaac gccgatttat ttgggcgact tctcaagtat
      841 actttcggcg gcgcggacat aaaaaaaccc cccgttttga ggcggggggc ctaatcctga
      901 tccggaattc ggggtggggg tgcacaaagg agagtccgaa ccaggcgcta caagccgaaa
      961 catagccgga tcgtttcgcc acaacaataa aagaccgata atcgtcaacc acctacggat
     1021 gggcggcgcc ggataaagga tttgtgacac gaacggcggg ccggaagcga cgagagacac
     1081 ggccgccgca cccacttcgc tgaaaggaac catggcaaaa caaaaaccct ggagcgggcg
     1141 tttcaccgaa cccaccgatg ccgtcgtcga ggccttcacc gcctcggtcg atttcgaccg
     1201 ccggctctac gtctacgaca tcatgggctc gatcgcgcac gcgcaaatgc tggcgaaggt
     1261 cggcgtgctc accaaaaaag agtgcgacgc catcgtcagg ggcctgaagg acatcgaagc
     1321 cgagatcgac gccggcaagt tcgactggag cgtggcgctc gaagacgtgc acatgaactt
     1381 cgaggcgcgc ctgatccagc gcatcggcga aaccggcaag aaactgcaca ccggccgctc
     1441 gcgcaacgac caggtcgcga ccgatctgcg gctttacctg cgtgacggca tcgacgtcgt
     1501 ggcccatgcc ctcgggcaac tgcaacaggc gcttctcgat ctcgccgagc gcgaggccgg
     1561 cacgcccatg cccggcttca cgcatctgca agtggcgcaa ccgatcacct tcggtcatca
     1621 tctgctggcg tggttcgaga tgctggcgcg cgacgccgag cgcctgcgcg attgccgcag
     1681 gcgcctgaat gtcatgccgt tgggcgccgc ggcgctggcc ggcacgagtt ttccgattga
     1741 ccgcgcttac accaccaaat tgctcggctt cgacgcgccc gcggagaact cgcttgacgc
     1801 cgtttctgac cgcgatttcg ccattgaatt cgttgcggct tgctcgctgc tcatgacgca
     1861 cctgtcgcgc atgtccgagg aactggtgct gtggtcttcg agccagttcg gcttcgtcga
     1921 actgtcggac gcctattgca ccggctcgtc catcatgccg caaaagaaaa atccggatgt
     1981 accggaactg gtacgtggca agaccgggcg cgtgaacgga cacctcgtgg ccttgctgac
     2041 gctcatgaag gcgcaaccgc tggcatacaa caaggacaac caggaagaca aggaaccggt
     2101 gttcgacacg ctcgacaccg tgctcggcag cctgcgggtg ttcgccggca tgattccgtc
     2161 catgaaggtg cggcgcgacc atctgctgcg cgcggcggtc atgggccatg ccaccgccac
     2221 ggatctcgcc gattatctgg tgcgccaggg ggttcccttc cgcgacgccc atgaaatcgt
     2281 gggcaaggcc gtgcggctgg ccatcgatac cgaccgcgac ctgtcgcaga tcgacctcga
     2341 cgaatacaaa aaactcagcc ccgccatcgg caaggacgtg tatggggtgc tgacggtgga
     2401 gggctcgctc aaggcgcgca atcatctcgg cggcaccgcg ccggcgcagg tgaaggcggc
     2461 catcaagcgc gcccgcaaac gcattacaaa gaaaagatag acaggattta caggataaaa
     2521 aaacacgatt aacaggatta cttctttaat ttttaaaatc cttaatcctg taaatcttga
     2581 aaaatcctgt taatcctgtc tatccgtttt attggagact attaagcccg acatggacac
     2641 cctcgaagac ctccggaaac gcctgcgcga gttcgcgcgc gagcgcgact gggagcagtt
     2701 tcactcgccc aagaatcttt caatggcgct gatcgtcgag gccgccgagc tggtcgagca
     2761 cttccagtgg ctgaccgagg ccgaaagccg cgcgctggca ccggaaaagc gcgagccggt
     2821 ggcgcaggaa ctggccgacg tattcatcta tctcaaccgc atggccgatc tgctcggcat
     2881 cgacctgatg gatgccgccc gccgcaagat tgaattgaac gccaagaaat atccggcgca
     2941 tgaagtgcgc gggaaggcgg ataagagata atgttgcgcc acgatggcaa tcgccgataa
     3001 cgagctcctg acgaa
//
LOCUS       DBNGZE010000417         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1516170, whole genome shotgun sequence.
ACCESSION   DBNGZE010000417 DBNGZE010000000
VERSION     DBNGZE010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1516170"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16FV_10690"
     CDS             <1..719
                     /locus_tag="AC16FV_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR2850554.1"
                     /translation="FNRNLVATLDYSITSDWGVTVQAPWVDRQHWHIHNHQGAQVPES
                     WDFSGLGDMRVTARHQFTSSNESLNTRGVTAGLKLPTGKTYVVNDDGDPAERTLQPGS
                     GTTDLIAGMYLNGPMNFGTQIATGFLQVQIQYPLNARDDFRPGNQYLFDLGMAYPLTP
                     AWSGLLQLNTQIKDRDRGGEAEPRDSGGSFVWLSPGLSYALTRTVKFYGFVQLPLYQH
                     VNGIQLTAGWTAAAGASWRF"
     gene            848..>2663
                     /locus_tag="AC16FV_10695"
     CDS             848..>2663
                     /locus_tag="AC16FV_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR2850555.1"
                     /translation="MENNNSEAHPQKSSTAGLTLLALGVVYGDIGTSPLYAVKETFGP
                     SHGIALETANILGGLSTIFWSLMIVVTFKYVMLIMRANNKGEGGIMALLALASSAVKD
                     QPRWRMLILAFGVVGASLFYGDAVLTPAISVLSAVEGLEVGTAAFKPYVIPLAVIVLV
                     TLFMFQRHGTAVVGAFFGPICALWFLALGVVGIHHIVENPIVLQAVNPLHALRFVTGH
                     GFASFVVLGAVLLAFTGAEALYADMGHFGKRAVRIAWFGLVLPALVLNYFGQGALLIA
                     TPQAIDNPFYLAYPGWALYPMIGLATAATVIASQATISGAYSITKQAIQLGYLPRFNI
                     QHTSAKQIGQIYLPAVNWVLLAAVLAAVVGFGSSSRLASAYGVAVVGTMLITTFLTFF
                     VIRYSWGYSLWLCLLATGSFFIVDLAFFSASLLKVFDGGWFPLVMGGAVFTLMVTWRR
                     GREILLARLRESSVPLDMFLDSLMRHPPTRVPGTAVFLTSTHGAVPHALLHNLAHNKV
                     LHERVVFLTVVIKDVPWVPAKERVRIKDLGNNCFQLIVDFGFKDRPDVMQALELCREH
                     EMEFQPLQTSFFLSRETVIASIRYEGMALWRERLFSTLA"
BASE COUNT          445 a          861 c          805 g          552 t
ORIGIN      
        1 cgttcaatcg caatctggtc gccacactgg attacagcat cacctccgat tggggcgtga
       61 cggtacaggc gccgtgggtc gatcgtcagc attggcatat tcacaaccat cagggcgcgc
      121 aggtgcctga gagctgggac ttttcagggc ttggtgacat gcgcgtcacc gcccggcatc
      181 agttcacaag cagcaacgaa tcgctgaata cccgtggcgt gactgccgga ctgaaactgc
      241 cgacaggaaa aacttacgtc gtcaacgacg acggcgatcc cgccgagcgc acactgcaac
      301 ccggcagcgg caccaccgat ctgatcgccg gaatgtacct caacggtccg atgaatttcg
      361 ggacacaaat cgccaccgga ttccttcagg tgcagattca atatccgctt aacgcccgcg
      421 atgacttccg tccgggcaat cagtatctgt tcgatctcgg aatggcctat cccctgacgc
      481 ccgcctggag cggtctgctg caactcaata cgcagatcaa ggatcgcgat cgcggcggcg
      541 aggccgagcc gcgggacagc ggcgggagct tcgtgtggct gagcccgggc ctgagttatg
      601 cgctgacccg cacagtcaag ttctacggtt tcgtgcaatt gcccctgtat cagcacgtca
      661 acggcattca actcaccgcg ggatggacgg cggcggccgg cgcgagctgg cgcttctaag
      721 tacggctatc tgccgggttt ttcctttatc gcccgttggc ctgctgcata caagccttgt
      781 cggcttgcaa ctcccggggt gacggagtaa cgtgcgcgcg cattcgcccc ctcccgggag
      841 atttggattg gaaaacaaca acagcgaagc gcacccgcag aaatcctcca ccgccggact
      901 caccctgctc gccctcggcg tcgtttacgg cgacatcggc accagcccgc tctacgccgt
      961 caaggaaacc ttcggcccga gccacggcat cgcgctcgaa accgccaaca tcctcggcgg
     1021 gctttcgacc attttctggt cgctcatgat cgtggtcacg ttcaaatacg tgatgctcat
     1081 catgcgcgcc aacaacaagg gcgagggcgg gatcatggcg ctgcttgcgc tcgcctcgtc
     1141 cgcggtcaaa gaccagccgc gctggcgcat gctgatcctg gcgttcggcg tggtcggggc
     1201 ctcgctgttc tacggcgacg cggtactgac gccggcgatc tcggtgctgt ccgcggtaga
     1261 ggggctcgag gtcggcacgg cggcattcaa gccttacgtc attccgctcg cggtcatcgt
     1321 gctggtgacg ctgttcatgt tccagcgtca cggcacggcg gtcgtcgggg cgttcttcgg
     1381 gccgatctgc gcgctgtggt tcctcgctct cggcgtggtc ggaatccacc acatcgtcga
     1441 gaatccaatc gtgctccagg ctgtcaaccc gctgcacgca ctgcgcttcg tcaccggaca
     1501 tggtttcgcc tcgttcgtgg tgctcggcgc ggtgctgctg gccttcaccg gcgccgaagc
     1561 gctgtacgct gacatgggtc atttcggcaa gcgcgcggtg cgcattgcgt ggttcggtct
     1621 ggtgctgccg gcgctggtgc tcaattattt cggccaaggc gcgctgctga tcgccacccc
     1681 ccaggccatc gacaacccgt tttacctcgc ttatccgggc tgggcgctgt acccgatgat
     1741 cgggctcgcg accgcggcca ccgtcatcgc ctcgcaggcg acgatctctg gcgcgtactc
     1801 gatcaccaag caggcgatac aactcggtta cctgccgcgc tttaatattc agcacacctc
     1861 tgcgaagcaa atcggacaga tttatcttcc ggccgtgaac tgggtactgc tggccgcggt
     1921 gctggccgcc gtggtcgggt tcggctcgtc ctcccgtctc gcctcggcct acggcgtggc
     1981 agtcgtgggc acgatgctga tcaccacctt tcttaccttt ttcgtgatcc gctacagttg
     2041 gggttacagt ctgtggttgt gcctgctcgc caccggttcg ttcttcatcg tggacttggc
     2101 gttcttctcg gccagtctgc tcaaggtatt cgacggcggc tggtttccgc tggtgatggg
     2161 cggagcggtg ttcacgctca tggtcacttg gcggcgcgga cgcgaaatcc tgctggcgcg
     2221 gctgcgggaa tcctcggtgc cgctcgacat gttcctggac tcactgatgc gccacccgcc
     2281 aacgcgggta cccggcacgg cggtgttcct gacctcgacc cacggagccg tgccgcacgc
     2341 gctgctgcac aacctcgcgc acaacaaggt gctgcacgaa cgcgtggtct tcctcacggt
     2401 ggtgatcaag gacgtgccgt gggtgccggc gaaggaacgc gtccgcatca aggacctagg
     2461 aaacaactgc ttccagctta tcgttgactt cggcttcaag gatcggcccg acgttatgca
     2521 ggcgctggaa ctgtgccgcg agcacgagat ggaattccag ccgcttcaga cctcgttctt
     2581 cctcagccgc gagaccgtga tcgcgagcat ccgctacgaa ggcatggcgc tgtggcgcga
     2641 gcgcctgttt tccacactgg cgc
//
LOCUS       DBNGZE010000418         8348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1377934, whole genome shotgun sequence.
ACCESSION   DBNGZE010000418 DBNGZE010000000
VERSION     DBNGZE010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1377934"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="queG"
                     /locus_tag="AC16FV_10700"
     CDS             <1..922
                     /gene="queG"
                     /locus_tag="AC16FV_10700"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR2850556.1"
                     /translation="HGEMHYMESHGTRRSRPQELQPGTLRVISARLDYLPGQAANSET
                     VMRGPKQGFISRYALGRDYHKVLRSRLQKLADRIESQVGPFQYRAFTDSAPVLEKALA
                     EKAGLGWIGKHTNLIDKQTGSWFFLGELYTDLPLPVDTPAENHCGTCHACLDVCPTRA
                     IVAPYELDARRCISYLTIELRGPIPIELRPLIGNRIYGCDDCQLICPWNRFAHPTAEP
                     DFAPRHGLDAPELIRLFSWTEEEFLKYTEGSAIRRIGYECWLRNIAVALGNMPMNEPV
                     IEALQARSNHPSELVREHVVWALRQHGVIC"
     gene            complement(994..2391)
                     /locus_tag="AC16FV_10705"
     CDS             complement(994..2391)
                     /locus_tag="AC16FV_10705"
                     /inference="COORDINATES: protein motif:HMM:NF048867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR2850557.1"
                     /translation="MAMGDNVSIVHLKWRGSLQFARCFMSSTSAVRVPILTGAIFYVA
                     LLLAGCFDSGGGGSVSDTTPPAVIATNPADGATGVFADGSIRITFSELIDPTTLNSTT
                     FRLETASHTPVLIAVNYAGDIVTFTPVSPQPLARGTVYIASITTGIKDIAGNAITAAL
                     SWSFTTETDVWQTTNVSGAPTGRTKHTAVWTGSEMIVWGGQDSGGASNSGGRYDPVTN
                     TWVATSLSSAPAPRFEHTAIWTGTRMIVWGGWDGGFNYFNNGAAYDAVNGWSPIASLP
                     SFSARRNHTAVWTGTEMIVMGGFTGLDFTNTSAAYNPATNTWRSLADPPAGFFGRSNH
                     TAVWTGTEVIVWGGVTSVGVTNTGAAYDPATDSWRLISTTDAPTPRLDHTAVWTGPAG
                     IAMVIWGGRDGFGNRLNDGGRYDPVTDRWRATDLTINFPTARFDHTAVWTGSRMIVWG
                     GDDLTFANTGGRYAP"
     gene            complement(2428..2991)
                     /gene="orn"
                     /locus_tag="AC16FV_10710"
     CDS             complement(2428..2991)
                     /gene="orn"
                     /locus_tag="AC16FV_10710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010845276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR2850558.1"
                     /translation="MAQSPNGAQDSNALIWIDMEMTGLNPDDDRIIEVATIITDSALS
                     VIAEGPVLAVHQSDAVLASMDDWNQRTHAGTGLIERVKQSTYNEADVERLTLEFLQKY
                     VPRNKSPMCGNSICQDRRFLARTMPQLEAWFHYRNLDVSSIKELVKRWKPSLCSGVVK
                     KNTHKALDDIRESIEELKYYREHFFAK"
     gene            3147..4388
                     /locus_tag="AC16FV_10715"
     CDS             3147..4388
                     /locus_tag="AC16FV_10715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR2850559.1"
                     /translation="MNTFSIIFISLLGLSLLMELWLANRHIRHVNANRDAVPNAFRRK
                     ISLEEHRKAADYTIAHTRFGRLEDIYGTALLAIWTVGGGLEWLDRLVRGLGHGELVTG
                     TLFILSAFVVMGLIELPVSIYQTFVIENRFGFNKTTPVLFFTDLVKKTLLLGIIGTPL
                     AIAALWLMQETGALWWVYVWLLWSGFTLFLVWAYPAWIAPIFNKFTPLKDNSVRERIQ
                     ALLKRTGFRSRGIFVMDGSRRSAHGNAYFTGFGKSKRIVFFDTLLEHLGEKEIEAVLA
                     HELGHFKRRHVIKRMFLTFGMSLFGLALLGWLIAQPWFYAGLGMTQPSTHAALMLFML
                     VVPVFTFFLQPLFAWSSRRYEYEADSFAAEQTDAQELVSALVKLYKENASTLTPDPLH
                     SAFYDSHPPAALRIAHLAGKT"
     gene            4410..4697
                     /locus_tag="AC16FV_10720"
     CDS             4410..4697
                     /locus_tag="AC16FV_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR2850560.1"
                     /translation="MLKTLALFPVLVVLSVSAQAATLPGNAAEGKKLHDANCMSCHND
                     SVYKKKDRSVKDIDGLVQQINSCGHAGNITIGKQQANDLVKYLNETYYKFK"
     gene            4710..5045
                     /locus_tag="AC16FV_10725"
     CDS             4710..5045
                     /locus_tag="AC16FV_10725"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR2850561.1"
                     /translation="MSNLTAKSCKPCAPGTPPLSREQATGMLAQVPGWSLNDAATELS
                     RTFKFKNYHETMAFVNAQAWVAHREDHHPDLEVGYNRCHVRFSTHSIKGLSENDFICA
                     AKLNALTGE"
     gene            5231..5479
                     /locus_tag="AC16FV_10730"
     CDS             5231..5479
                     /locus_tag="AC16FV_10730"
                     /inference="COORDINATES: protein motif:HMM:NF022577.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR2850562.1"
                     /translation="MTGSVLHKYRNAGMLDRALRTIIGVVMIYFGFINTGLIGQVILA
                     VLIGIFGIINILVAVTGVCPVYTLAGINTCTSREAGQH"
     gene            5483..7375
                     /locus_tag="AC16FV_10735"
     CDS             5483..7375
                     /locus_tag="AC16FV_10735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR2850563.1"
                     /translation="MKLRLRLLVSFIAFALFTLLIFGTSAYQIAVDSAQKRETELLLQ
                     FVTDQAGTMTGGKEIRRWKNTPGSSVLARNEYLLLVAGDNGELRPAPDFQVESSDARN
                     RYDLMLSQTRSGQKNGIFESGHQTFIWASAPIGTGGYHLAALRPLRNELASLWSTFGI
                     RLVAAGIVIVWIAVWGALILAGLFVKNQEKHNAILIHQAMHDELTRLPKRNLLYDRLQ
                     QAIHQAQRARHHAALLIMDLDRFKEINDTLGHHSGDELLKQIGNRLLHSLRKSDTVAR
                     LGGDEFAILAPGCHVEQAVQLANKIALIMDPPHPINGMEVGIQPSIGIAVYPQHGEDV
                     DSLIRHADVAMYQAKQQGGGWTVYASESDPHSLQRLTLMQDLRQAIESESLMLYYQPE
                     LDLSTGRVTGVEALARWNHSQRGLIPPDEFIGIAELSGQINALTALVLRQAFQQYHAW
                     RRLGIRLAISINLSARNLHDTGLPAYIGKLLEAWNVTPSDIRLEITESAMLTDPAKAK
                     RILSEFDAMGLGLAIDDFGTGYSSLAYLKQLPVNIIKIDKSFVMDMTHDEDDASIVRA
                     TIDLAHNLGLRVVAEGVETEEVLSALRELGCDHLQGYYISRPVPAHELTRWLATHHAA
                     PDAAAS"
     gene            complement(7467..>8348)
                     /locus_tag="AC16FV_10740"
     CDS             complement(7467..>8348)
                     /locus_tag="AC16FV_10740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2850564.1"
                     /translation="ATSDVLIAGCGDIGECVAMLERAAGRRVTGLVRSGVSARRLRAA
                     GIQPITADLDVPASLHDLPLRNALVYYFAPPPGEGVTDPRMETFVSILDSSGPPKRVV
                     LISTTGVYGDCQGEWVTEDRPPNPQADRARRRLAAETNLRHWSEKSGVSVIILRVPGI
                     YGRGYLPEERLRAGEPVLREEESPFSNRIHADDLARACFIAGHHDSPGILYNVSDGHP
                     TTMTDFFNRVADVLGIPRPPAITLEQARRQLGEGMLSYLAESKRIDNRRMREELGVEL
                     MYPDLASGLPSCFERCG"
BASE COUNT         1686 a         2646 c         2333 g         1683 t
ORIGIN      
        1 gcatggagaa atgcattata tggaatccca cggcacgcgc cgcagccggc cgcaggaatt
       61 gcagcccggc acgctgcgcg tcatttcggc gcgcctggat tatctgccgg gacaggccgc
      121 taatagcgag acggtcatgc gcggaccgaa gcagggtttc atctcgcgct acgcactcgg
      181 gagggattat cacaaggtcc tgcgatcccg tttgcagaaa ctggccgatc gcatcgagtc
      241 acaagtcggt ccgtttcagt accgggcctt caccgacagt gccccggtgc tggaaaaggc
      301 attggccgaa aaggccgggc tcggctggat cggcaaacat accaacctga tcgacaagca
      361 gaccggctcg tggttttttc tcggcgagct ttacaccgac ctgccgttgc cagtggacac
      421 gcccgccgag aaccattgtg gcacctgcca tgcctgtctc gatgtttgtc cgacccgggc
      481 catcgtcgcg ccctacgagt tggatgcgcg ccgctgcatt tcttatctca ctatcgagct
      541 gcgcggcccg attcccatcg agttacgccc gctgatcggc aatcgcattt acggttgcga
      601 cgactgccag ctcatctgtc cctggaaccg tttcgcccat cccactgcgg aacccgattt
      661 cgcgccacgc cacggcctcg atgcgccgga attgatcaga ctgttttcct ggaccgaaga
      721 ggagttcctg aaatacaccg aaggctcggc catccgacgc atcggctacg aatgctggct
      781 gaggaacatt gccgtagcgc tgggtaacat gccgatgaac gaaccggtca tcgaagcgct
      841 ccaggcacgt tcaaatcatc cttcagaatt ggtaagggaa catgttgtgt gggcgctgag
      901 gcagcatggt gttatatgct gaatcgcacg gttgatacag acctgtcaaa gatagctcag
      961 cgcgtacagt cacagcctgc gcaacgagct cggctatggt gcgtagcggc caccagtgtt
     1021 agcgaaggtt agatcatctc ctccccacac gatcatccgg cttccagtcc ataccgcggt
     1081 gtggtcaaat cttgccgtgg gaaaatttat cgttaaatcg gtcgctctcc atctgtcggt
     1141 caccggatcg taacgaccgc cgtcgttgag acgattaccg aagccatctc gtccgcccca
     1201 tatcaccatg gcgatacccg cgggtccggt ccagacggca gtgtgatcga gcctgggcgt
     1261 gggagcgtcc gtcgtggaaa tcagtcgcca agaatccgta gccgggtcat aagcagcacc
     1321 cgtgttggta actcctacgc tggtgactcc accccaaaca attacctccg tgccggtcca
     1381 cacggctgtg tgattcgacc tgccgaaaaa tcccgctggc ggatcggcga gggagcgcca
     1441 cgtattcgtc gcgggattgt aggccgcgct agtattggtg aagtccaagc cggtaaaccc
     1501 gcccatcacg atcatctcgg ttccggtcca cacggcagtg tggtttcgcc gcgctgaaaa
     1561 actcggtagc gacgcgatgg ggctccagcc attcacagcg tcatacgccg ccccgttgtt
     1621 gaaatagttg aacccgccgt cccacccgcc ccagacgatc atccgggtcc cagtccagat
     1681 ggccgtgtgc tcgaacctcg gtgccggtgc gctgctgagc gacgtggcaa cccaggtgtt
     1741 tgtaacagga tcatagcggc cgccgctatt gctggcaccg ccgctatcct gtccgcccca
     1801 tacgatcatt tcgctgccgg tccacaccgc cgtgtgcttg gtccggccgg ttggggctcc
     1861 actcacattt gtggtttgcc agacatccgt ttccgtcgtg aaactccagc tgagggcggc
     1921 ggttatggcg ttgccggcga tatccttgat gccagtggtg atgctcgcga tgtagacagt
     1981 gccacgcgcg aggggctggg ggctaaccgg tgtgaacgta acaatgtcgc cggcgtagtt
     2041 cactgctata agtaccggcg tgtgactcgc ggtctccaac ctgaaggtcg tagaattgag
     2101 cgtggtggga tctatcagct cgctgaacgt gatcctgatc gagccatcgg caaaaactcc
     2161 ggtggcccca tccgccggat tcgtcgcgat gacggcaggt ggtgtggtgt cagaaacact
     2221 accacctcct ccactgtcaa agcatcctgc aagaaggagt gccacataaa atatggcgcc
     2281 ggtcaggatg ggaacacgaa cagcggaggt tgaggacata aaacacctcg cgaactgcaa
     2341 gcttccccgc cattttaaat gcacgatgga gacgttgtca cccatcgcca ttttacagtt
     2401 tcgtttgaga ggcttacgta aagccgacta ctttgcgaag aaatgttcgc ggtagtactt
     2461 caattcctcg atcgactcgc gaatgtcatc gagcgccttg tgcgtgttct ttttcaccac
     2521 accgctgcaa aggctgggct tccagcgctt caccaactcc ttgatggagc tgacgtcaag
     2581 attgcggtaa tgaaaccatg cctcgagctg cggcatggtg cgcgcgagaa agcgccggtc
     2641 ctggcaaata ctgttgccgc acatcgggga tttgtttctg ggaacgtact tttgaaggaa
     2701 ttcaagcgtg agccgttcga catccgcctc gttataagtg gactgcttca cgcgctcgat
     2761 caggccggtt cccgcgtggg tacgctggtt ccagtcatcc atggacgcca gcaccgcatc
     2821 ggactggtgc accgccagca ccggtccttc cgcgatgacg ctcagcgcgc tgtcggtaat
     2881 aatggtggcg acttcgataa tccggtcgtc gtccggattt aaccctgtca tttccatgtc
     2941 tatccagatc agggcgttgc tgtcctgcgc cccgttgggt gattgcgcca tgcgtggtct
     3001 ccggattctt tggattctgt gcgggaattt tccgcgttct ctactgtctc ttcggtggtg
     3061 atgataacat ctaatggagc cctacttcgt tcgtcttgtc attatattag aagccctact
     3121 gcgttcgttt tgtcattata ttacttatga ataccttctc gatcatattt atcagtctgc
     3181 tcggcctctc gctgttgatg gagctgtggc tcgccaaccg gcacatccgc cacgtcaacg
     3241 ccaatcgcga cgcggtgccg aatgcctttc gccgcaagat ctcgctggaa gaacaccgca
     3301 aggccgccga ttacaccatc gcgcacaccc gcttcgggcg tcttgaggac atctacggca
     3361 cggccctgct ggcgatctgg accgtgggcg gcggactgga gtggctggat cgtctcgtgc
     3421 gcggcctcgg ccatggcgag ctggtcaccg gcacgctgtt tattctgagt gccttcgtcg
     3481 tcatgggcct gatcgaactc ccggtcagca tctaccagac cttcgtgatc gaaaaccgct
     3541 tcggtttcaa caaaaccacg cccgttttgt tcttcaccga tctcgtcaag aaaaccctgc
     3601 tgctcggaat catcggaacg ccgcttgcaa tagccgcgct gtggctgatg caggagaccg
     3661 gcgcattgtg gtgggtgtac gtgtggctgc tgtggagcgg cttcacgctg ttcctggtgt
     3721 gggcctatcc cgcatggatc gcgccgatct tcaacaaatt cacgccgctc aaggacaact
     3781 ccgtgcgcga gcgcattcag gcgctgctca agcgcaccgg cttccgctcg cgcggcatct
     3841 tcgtcatgga cggttcgcgc cgtagcgcgc acggcaacgc ctacttcacc ggcttcggca
     3901 aatccaagcg catcgtcttc ttcgacacgc tgctggaaca tcttggcgaa aaggagatcg
     3961 aggccgtgct tgcccatgaa cttggccact tcaagcgccg gcacgtcatc aagcgcatgt
     4021 tcctcacctt cggtatgagc ctgttcggtc tggccctgct cggttggctg attgcgcaac
     4081 cctggttcta cgccggcctc ggcatgacgc aaccctcgac ccatgccgcg ctgatgctct
     4141 tcatgctggt cgtgccggtg ttcaccttct tcctgcaacc gctgttcgcc tggagctcgc
     4201 gccgctacga atacgaggcc gacagtttcg ccgccgaaca gaccgatgca caggaactcg
     4261 tcagcgcgct ggtcaagctc tacaaggaaa acgccagcac gctcactccc gacccgctgc
     4321 attccgcttt ttacgactcg cacccgcccg ccgcgctgcg catcgcgcat ctggcgggca
     4381 aaacctgaca cataaaccaa ggaggaatca tgctcaagac actcgcatta ttcccggtac
     4441 tcgtagttct atccgtctcc gcccaagccg cgaccctgcc gggcaacgcc gccgagggca
     4501 agaagctgca cgatgccaat tgcatgtcct gccataacga cagcgtgtac aagaaaaagg
     4561 acagatcggt caaagatatc gacgggctgg tacagcagat caattcctgc gggcacgccg
     4621 gcaatatcac gatcggaaag cagcaggcaa acgacctagt gaagtatttg aacgaaacct
     4681 attacaagtt caaatagcgc gggcctgtca tgtcgaatct aactgccaag tcctgcaagc
     4741 cctgcgcacc cggcaccccg ccgctgtcgc gcgagcaggc gacgggcatg ctggcccaag
     4801 tgcccggctg gtcgctgaac gacgccgcca ccgaactcag ccgcaccttc aagttcaaga
     4861 attaccacga aaccatggcg ttcgtgaacg cacaggcatg ggttgcgcac cgcgaggatc
     4921 accacccgga cctcgaggtc ggctacaacc gctgccacgt gcgcttcagc acgcacagca
     4981 ttaagggcct gtcggaaaac gactttatct gcgccgccaa actcaacgcc ctgacgggcg
     5041 aataagcgat ccgacggccg ccgcgttcag acccggcccc ccggatcgcc ggtttttccc
     5101 cgatttttct ccggccgcgc ccaggttccc ggtcacgccc gcgcggcatg gtctagattt
     5161 aatacagacg tcctgtctcc acgctcccac ccggagcgcg gggttcaatc agactgcaag
     5221 gagattattc atgactggct cggttctcca taagtaccgc aacgcgggca tgctcgaccg
     5281 cgcgcttcgt accatcatcg gcgtcgtgat gatctacttc ggtttcatca ataccggtct
     5341 catcggtcag gtcatactcg cggtcctcat cgggatattc ggcatcatca atatcctggt
     5401 cgccgtcacc ggcgtctgtc cggtctatac cctggccggc atcaacacat gcacttcgag
     5461 agaggcgggt caacactaaa acatgaaatt gcgtttaagg ttgctggtct cgtttatcgc
     5521 ctttgcctta ttcacgcttc tcatcttcgg caccagcgcc tatcaaatcg ccgtcgacag
     5581 cgcccagaaa cgggaaacgg aattactgct gcagttcgtg acggatcagg ccggcacgat
     5641 gaccggcgga aaggaaatcc ggcgctggaa gaatacgccc ggatccagcg tgctggcccg
     5701 gaatgagtat ttgctgctcg ttgccggcga caacggcgaa ctcagaccgg cgccggactt
     5761 ccaggtcgaa tcatccgatg cgcggaaccg atatgacctg atgttgagcc agacccgctc
     5821 ggggcagaaa aacggcatct tcgaatccgg ccatcaaacc tttatctggg cctccgcgcc
     5881 catcggcacc ggcggctatc atctggcggc gctgcgccca ctgcgcaacg aactggcttc
     5941 gctctggagc accttcggca ttcgcctcgt cgccgccggc atagtcatcg tctggatcgc
     6001 cgtgtggggt gccctgatcc tcgccggact gttcgtcaag aatcaggaaa aacacaacgc
     6061 catcctgatc caccaggcca tgcacgacga gctgacccga cttcccaagc gcaatctgct
     6121 ctacgatcgg ctgcaacagg cgattcatca ggcacagcgc gcgcgtcacc acgccgcgct
     6181 cctgatcatg gacctcgacc ggttcaagga aatcaacgac acgctcggcc atcacagcgg
     6241 cgatgaactg ctgaaacaga tcggaaaccg gctgctgcat tccttgcgca aatcggacac
     6301 ggtggcgcgc ctcggcggcg acgaattcgc catcctggcg cccggctgtc acgtcgaaca
     6361 ggcggtgcag ttggcgaaca agatcgccct catcatggac ccgccccatc cgatcaacgg
     6421 aatggaagtc ggcattcagc cgagcatcgg catcgccgtc tatccgcagc acggcgagga
     6481 cgtcgactcg ctcatacgac acgccgatgt cgcgatgtac caggcgaaac agcagggcgg
     6541 cggatggacg gtatatgcgt cggaatcgga tccgcacagc ctgcaacggc tcaccctcat
     6601 gcaggatctt cgtcaggcga tcgagagcga gagcctgatg ctctactacc agccggagct
     6661 ggatctgtcc acgggacgcg tgaccggcgt ggaagccctg gcgcgctgga atcattcgca
     6721 aaggggactc atcccgccgg atgaattcat cgggatcgcg gaactgagcg ggcagatcaa
     6781 cgccctcacc gcgctggtgc tgcgccaggc gtttcagcaa taccacgcgt ggcggcggct
     6841 cggcatacgt ctcgccatct caatcaacct gtccgccaga aacctgcacg acacggggct
     6901 gccggcctac atcgggaaac tgctcgaagc ctggaacgtc acgccttcgg acatccgcct
     6961 cgagatcacc gagagtgcga tgctcaccga cccggccaag gccaaacgca tcttgtccga
     7021 gttcgatgcc atggggctgg gactggccat cgacgatttc ggcacgggtt attcctcgct
     7081 cgcctatctc aagcaactgc cggtcaacat catcaagatc gacaaatcgt tcgtgatgga
     7141 catgacccac gacgaggacg acgcttccat cgtgcgcgcc acgatcgatc tcgcgcacaa
     7201 tctcggactg cgggtggtcg ccgaaggcgt ggaaacggag gaagtcctgt cggccctgcg
     7261 ggaactgggc tgcgaccatc ttcagggtta ctacatcagc cggccggtgc cggcacatga
     7321 actgacgcgc tggctggcaa cccaccacgc cgcgcccgac gccgcggcat cctgaatctt
     7381 ccggtggcac gttgaaccgt cacgttccag cgtgcacgcc atcaagcccg tgccccatag
     7441 acgggtaacc gtcacccttc ctcgacttat ccgcatcttt caaaacacga cggcaggccg
     7501 gacgccaggt ccgggtacat caattccacg cccaattcct cccgcatgcg tcggttgtcg
     7561 atgcgtttgg attccgccag ataagaaagc atgccttctc ccagctgccg gcgcgcctgc
     7621 tccagcgtga ttgccggtgg gcgggggatg cctagcacat ccgccacacg gttgaaaaaa
     7681 tccgtcatgg tcgtgggatg cccatcactg acgttataca ggatgccggg actgtcatga
     7741 tgcccggcga tgaagcaggc ccgcgccaga tcgtcggcat ggatacgatt gctgaacggc
     7801 gattcctctt cgcgcaacac tggctcaccc gcgcgcaacc gttcctcggg caagtaacct
     7861 cgaccataaa tacccggcac acgcagaatg atgaccgata cgccgctctt ctcgctccag
     7921 tgtcgcaggt tggtctccgc cgcgagccgc cgcctggcgc gatccgcctg cgggttgggt
     7981 ggccgatcct ccgtcaccca ctctccctga caatctccat agacacctgt cgtgctgatc
     8041 agcaccacgc gtttcggcgg cccggatgaa tcgaggattg aaacaaaagt ttccatgcgc
     8101 gggtcggtca cgccctctcc cggcggtggc gcgaagtaat aaacgagcgc attcctgagg
     8161 gggagatcat gcaaggacgc cggcacgtcg agatcggcgg ttatcggctg aatgccggcg
     8221 gcgcgcaatc gccgggcgga taccccggag cgaaccagac ccgtgacgcg gcgacccgcg
     8281 gcacgttcca gcatggcaac gcactcaccg atgtcgccac aacctgcaat cagcacatca
     8341 gatgtagc
//
LOCUS       DBNGZE010000419         4051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_57868, whole genome shotgun sequence.
ACCESSION   DBNGZE010000419 DBNGZE010000000
VERSION     DBNGZE010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_57868"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC16FV_10745"
     CDS             complement(<1..292)
                     /locus_tag="AC16FV_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR2850565.1"
                     /translation="MIKKILVANRGEIAVRIVRACAEMGIKSVAIYTDADRHALHVKK
                     ADEAYNVGPDPVGGYLNAHRIVNLAVATGCDALHPGYGFLSESPQLAEICARR"
     gene            565..1071
                     /locus_tag="AC16FV_10750"
     CDS             565..1071
                     /locus_tag="AC16FV_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR2850566.1"
                     /translation="MPGTIDPIQLAERGAHLTGTLPLKSMPRLAQGSLEGSGDVAVDL
                     AFEREEGEQVFVMHGTLRVPLRVTCQRCLEAMDLNLEAAPWLILLKSGERLQRHEDDA
                     DILVADKPLSLSSLVEDELLLALPMVPAHAADQCPVKVRAASVAPGQTERGGKNPFSV
                     LDRLKKTK"
     gene            1104..1283
                     /gene="rpmF"
                     /locus_tag="AC16FV_10755"
     CDS             1104..1283
                     /gene="rpmF"
                     /locus_tag="AC16FV_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR2850567.1"
                     /translation="MAVQKSRVSPSRRGMRRSHDALKKSTLSVDKTSGETHRRHHVTA
                     DGYYRGRKVLETKSE"
     gene            1322..2341
                     /gene="plsX"
                     /locus_tag="AC16FV_10760"
     CDS             1322..2341
                     /gene="plsX"
                     /locus_tag="AC16FV_10760"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR2850568.1"
                     /translation="MITIALDAMGGDHGVGVTVPAALQTLKHQTALKLILVGQREAVE
                     AELRRHHATASDRLAVHHASETVGMDEPPAQALRNKKDSSMRVAINLVKEGAAQAAVS
                     AGNTGALMATARFVLKTLPGIDRPAIITTLPTMHDHVHVLDLGANVDCAPEQLLQFAI
                     MGSILVAAVEGKSRPRVGLLNVGEEDIKGNEIVKKTHELLRASSLNYAGFVEGDEIYT
                     GDLDVIVCDGFVGNIMLKTSEGLARMITHFMKQEFKRNLLTKLSALVALPVLQAFRKR
                     VDPRRYNGATFIGLTGTVIKSHGGADVLAYEHAILEAVAEVKNNVPERIGQELAAMAA
                     KGAVA"
     gene            2338..3324
                     /locus_tag="AC16FV_10765"
     CDS             2338..3324
                     /locus_tag="AC16FV_10765"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR2850569.1"
                     /translation="MSHKTGAVYSRIIGTGGYLPAKVLTNHDLEKMVETSDQWIVERT
                     GIRERHIAAEGETTASMGEQAARRAIEAAGITAKDIDLIIVATTTADQIFPSTACLIQ
                     ERLGIHGCAAFDVQAVCTGFVYALGVADKFVRTGSARCALVIGSETLSRITDWTDRTT
                     CVLFGDGAGAVILTASGKPGIISSHLHADGKYKDLLSVPAGISKGYDKVVNGTAYMKM
                     EGNEVFKVAVNTLGRIVDETLAANHMKKSDVQWLVPHQANHRIIAATAKKLGMSMDHV
                     VLTVDRHGNTSAASIPLAFNEAVRDGRIKAGDVVMMEAFGGGFTWGSVLLKY"
     gene            3470..>4051
                     /locus_tag="AC16FV_10770"
     CDS             3470..>4051
                     /locus_tag="AC16FV_10770"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR2850570.1"
                     /translation="MALAFVFPGQGSQSVGMMNALAAEFPVVKQTFAEASQALGKYLW
                     QLVEQGPEDKLNQTEITQPVMLAAGVATWRAWLAANGPRPQFMAGHSLGEYSALVCSE
                     ALDFSEAMKLVADRARFMQQAVPAGQGAMAAILGMEDDAVRKLCEQAAQGEVLEAVNF
                     NSPGQVVIACAVAAVARAVEQSKAAGAKRAVLLP"
BASE COUNT          818 a         1193 c         1275 g          765 t
ORIGIN      
        1 cgcggcgggc gcagatttcg gccaactgcg ggctttccga gaggaagcca tagccgggat
       61 gcaaggcgtc gcagccggtg gccaccgcca ggttgacgat gcgatgcgcg ttcaaatagc
      121 cgccgaccgg atcggggccg acgttatagg cctcgtccgc ctttttgacg tgcagcgcat
      181 gccgatcggc atcggtgtag atcgcgaccg acttgatacc catctcggcg caggcgcgca
      241 cgatgcgcac cgcgatttca ccgcggttgg caaccagaat ctttttaatc aagctgaaac
      301 tccgctgagc tgacctgccg gcgcccgtta cggcgccgtg cctcgttttg tacggaaagg
      361 gggcggtgct gtcaacaaca tttaccgccc actggcgcgt ctaaaaccct attttgacac
      421 aagtttgccg caaatatatg atgcgcggcc tgtatctggc cgcctgcccg caaaaccgtc
      481 cgattccgga ggttaagccc gcaacggtta tgaccggttg gtgagggtat catgtcgtca
      541 tcctggaaag agtcacatgg acatttgccg gggaccatag atccgatcca gctcgcggaa
      601 aggggcgcgc acctgaccgg cacgctgccg ctgaagagca tgccgcgcct ggcgcaaggc
      661 agcctggaag gatcgggcga tgtcgcggtc gatctcgcgt ttgagcggga ggaaggcgag
      721 caggtgtttg tcatgcatgg cacactgcgc gtgccgctgc gagtcacctg ccagcgctgt
      781 ctggaggcga tggacctgaa tctcgaggcc gcaccgtggc tcatcctgtt gaagtcggga
      841 gagcgtctgc aaagacacga ggacgatgcg gatattctgg tggcggacaa accgctgtca
      901 ttgagcagcc tggtcgagga cgaattgctg ctggccttgc cgatggtgcc ggcgcatgca
      961 gcagaccaat gtccggtcaa ggtccgcgcc gcgagtgtcg ccccgggcca gacagaacgc
     1021 ggtggcaaga acccgttttc ggttctggac cggttgaaga aaacgaaata gtgccaatta
     1081 tttttaattg acgaggattt accatggccg tacagaaaag ccgagtatca ccttcccgcc
     1141 gcggcatgcg tcgttcgcat gatgcgctca agaagtcgac gctttcggtc gacaagacca
     1201 gcggcgagac gcatcgccgg catcatgtca ccgccgacgg ttattatcgc ggccgcaaag
     1261 tgctggaaac caagtcggaa tagcatcggt ttgaaccgaa actgctcagg gacttgagaa
     1321 cgtgatcacg attgccctcg acgccatggg gggtgatcac ggtgtcggcg tcaccgtgcc
     1381 cgcggccctc cagacgctga aacatcagac ggcgttgaag ctcattctcg tgggccagcg
     1441 cgaggccgtt gaggccgagc tgaggcgtca tcatgcgacc gcgagcgacc ggcttgccgt
     1501 ccatcatgcc tctgaaaccg tcggcatgga cgaacccccc gcgcaggccc tgcgcaacaa
     1561 gaaagattcc tccatgcgcg tggccatcaa tctcgtcaag gaaggcgcgg cccaggcggc
     1621 tgtgtcggcc ggcaataccg gcgcgctcat ggccaccgcg cgcttcgtgc tcaagaccct
     1681 gcccggtatc gatcgcccgg cgattatcac gacgctgcca accatgcacg atcatgtgca
     1741 cgtgctcgac ttgggcgcca acgtggattg cgcgccggag cagctgttgc agttcgccat
     1801 catgggttcg atcctggtcg ctgccgtgga aggcaagtcg cgcccgcgcg tgggattgct
     1861 caacgtcggc gaggaagaca tcaagggcaa tgagatcgtc aagaagacgc atgaattgct
     1921 gcgcgccagt tcgctcaact acgccggttt cgtcgagggc gacgagatct acaccggtga
     1981 tctggatgtg atcgtgtgcg acggctttgt cggtaatatc atgctcaaga ccagcgaggg
     2041 gttggcgcgc atgatcacgc atttcatgaa acaggaattc aaacgcaacc tgctcacgaa
     2101 gctgtccgcg ctggtggcgt tgccggtgct gcaggcattc cgcaagcgcg tcgatccgcg
     2161 ccgttacaac ggggcgacat tcatcggact caccggcacg gtcatcaaga gccacggcgg
     2221 cgctgacgtg ctggcctatg aacatgccat tctggaggcc gtagcggaag tgaagaacaa
     2281 tgtccccgaa cgtatcgggc aggaactggc ggccatggcg gcgaaggggg ctgtggcgtg
     2341 agtcacaaaa ccggcgccgt ctattcgcgc atcatcggca ccggcggcta tctgccggca
     2401 aaggtgctaa ccaaccacga tctcgaaaaa atggtcgaga cctcggacca gtggatcgtc
     2461 gaacgcaccg gtattcgcga acgtcatatc gccgccgagg gcgagactac cgcttccatg
     2521 ggcgagcagg ccgcgcggcg tgccatcgaa gcggctggta ttaccgccaa ggacattgac
     2581 ctgattatcg tcgccaccac cacggccgat cagatatttc ccagcaccgc ctgcctgatt
     2641 caggaacgtc tcggtattca cggctgcgcc gctttcgacg tgcaggccgt ttgcaccggt
     2701 tttgtctatg cactcggcgt ggccgacaag ttcgtgcgta ccggctcggc caggtgcgcg
     2761 ctggtgatcg gcagcgaaac cctgtcgcgc atcaccgact ggaccgatcg caccacctgc
     2821 gtgttgttcg gtgacggcgc cggcgccgtg atcctgaccg catctggcaa gcccggcatc
     2881 atttcctcgc acctgcatgc cgatggcaag tacaaggacc tgctgagcgt tcccgccgga
     2941 atctctaagg gttacgacaa agtcgtcaat ggtacggcct acatgaagat ggaaggcaac
     3001 gaagtgttca aggtggcggt gaacacactc gggcgcatcg tggacgaaac actcgcggcg
     3061 aatcacatga aaaaatccga cgttcaatgg ctcgtgccgc atcaggccaa tcaccgcatc
     3121 atcgccgcga ccgcgaagaa actgggcatg tccatggatc acgtggtgct gacggtggac
     3181 cgccacggca atacctcggc ggcctcgatc ccgctggcct tcaatgaagc ggtgcgcgac
     3241 gggcgcatca aggccggtga tgttgtcatg atggaagcct tcggcggcgg tttcacctgg
     3301 ggctcggtgc ttttgaaata ttgatttgaa ccgccagggc gccggggtcg ccaaggaaca
     3361 tcaaagtaaa gaacacatct tgaaaaacat cttgagcaat ctttttctta aatcttttcc
     3421 tggcgctcct ggtgtcctgg cggtatacaa aatataaaaa ggatttttca tggcgcttgc
     3481 attcgtattt ccggggcagg gctcgcaatc ggtcggcatg atgaatgccc tggcggcgga
     3541 gtttcctgtc gtcaagcaaa ccttcgccga ggcctcgcag gccctgggca agtacctctg
     3601 gcaactggtg gagcaggggc cggaggacaa actgaatcag accgaaatca cgcagccggt
     3661 gatgctggcg gcgggtgtcg cgacctggcg cgcatggctg gcggcaaacg gcccgcgacc
     3721 gcaattcatg gccggccaca gcctgggcga atactccgcg ctggtgtgct ccgaagcgct
     3781 cgatttttcc gaggccatga aactcgtcgc ggaccgtgcg cgtttcatgc agcaggccgt
     3841 gccggccgga cagggcgcca tggcggcgat tctgggcatg gaagatgacg ccgtgcgtaa
     3901 actttgcgag caggcggcgc agggcgaggt gctcgaggcg gtgaatttca attcgcccgg
     3961 gcaggtcgtg attgcctgcg ctgttgccgc cgtggcgcgc gccgtggaac aatccaaggc
     4021 cgccggcgcc aagcgcgccg tgttgctgcc g
//
LOCUS       DBNGZE010000420         4398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1932823, whole genome shotgun sequence.
ACCESSION   DBNGZE010000420 DBNGZE010000000
VERSION     DBNGZE010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1932823"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1558)
                     /locus_tag="AC16FV_10775"
     CDS             complement(125..1558)
                     /locus_tag="AC16FV_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR2850571.1"
                     /translation="MSPLFAFGDEKPAAEPAAAAAAAPAPAAAPAPTPNKGDTSWMLV
                     STALVLMMSVPALALFYGGMVRSKNALSVLMQVFVVFSLITVLWCVYGYSLAFTEGSG
                     SLSAYFGGFDRLFLKGTFDSASGAFSMGATFSKATPITELVFVAFQATFAAITCALIL
                     GAFVERVKFSAVLIFMVLWFTFSYAPIAHLVWFWMGPDAYTAANVVDAMNAKAGMLWQ
                     WGALDFAGGTVVHINAGVAGLVGAYLIGKRIGFGKEHMAPHNLTLTMIGASLLWVGWF
                     GFNAGSALEANNSAALAFMNTFLATACAVLSWTCGAWIFKSKPSMLGAASGAVAGLVA
                     ITPAAGNVGIPGAFVIGTAAGLVCLWGVNQLKSWLKADDSLDVFGVHAVGGILGALLT
                     GVFNSPALGGPGLVTDWVTGATGYPGIGAQLLIQAKAVGVTIVWTAVVAFIAYKIADW
                     TVGLRVTAEEEREGLDTTSHGESAYRI"
     gene            complement(1635..1973)
                     /locus_tag="AC16FV_10780"
     CDS             complement(1635..1973)
                     /locus_tag="AC16FV_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR2850572.1"
                     /translation="MKLVTAIIKPFKLDDVRDALAEVGVQGVTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAIDAKQLDRVIEAITKSARTDKIGDGKIFVYDLEQVVRI
                     RTGETGKDAL"
     gene            complement(2025..3188)
                     /locus_tag="AC16FV_10785"
     CDS             complement(2025..3188)
                     /locus_tag="AC16FV_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019268.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2850573.1"
                     /translation="MNFCKTILAASIAAASVSTVMAAEKMPEKPAVPTLGQVMEATGI
                     SVNGYIDTSYTYLSGTGTFTSGTANRVFDTERRSFNINALDLTLSALPPEGFGGMVNL
                     TAGSDAKVITSAGTTVDEFDVTQAYVHFAKGGLMLIGGKYVTLSGAEVIKSPANLNFS
                     RSILFGYAIPFAHTGVRAYFTPGGSAVSPDGKWTFIAGLNNGWDVQSESQQTAAADGS
                     KGLGKTIELGANGSLTKSLSLAAVYYSGNESAGAATTGRRDLLDIVATFTATDNLSFV
                     LNYDKAKQEDAIAVGSDAKWNGLAGYANYKLSDAWRTSFRAESFDDKNGFRTGVTQKW
                     KEYTLTLANTPAKNVELRGEVRHDSSNQSSFNQPDGTTKKSQSSLGLEAIYKF"
     gene            3451..3687
                     /locus_tag="AC16FV_10790"
     CDS             3451..3687
                     /locus_tag="AC16FV_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR2850574.1"
                     /translation="MIDSKIIDQFVTAITRLLPEGPKGFEKNLRAAMQGVFDRMNLVS
                     REELEVQEQVLQRTRARLQELEKRIAELEAKLKK"
     gene            3771..>4398
                     /locus_tag="AC16FV_10795"
     CDS             3771..>4398
                     /locus_tag="AC16FV_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR2850575.1"
                     /translation="MSLAVVHSRAQIGLEAPPVTVEVHLANGLPSLSIVGLPEAAVKE
                     SKDRVRAALQNARFEFPARRITVNLAPADLPKEGGRFDLPIAIGILVASGQVSNHQLD
                     KYELIGELALTGQLRPVLGALTVALQTRHNGRALILPDANAQEAALAGGADIRAAKHL
                     LEVTAHFNSEKFLARHEAVMPAPEVSVIPDLRDVRGQHQARRALEIAAA"
BASE COUNT          913 a         1348 c         1314 g          823 t
ORIGIN      
        1 ttggacagtg gagcgcagcg acgctggcct gagccgcgac agcggcgatg gcgagcccca
       61 tggatggagc gagcaaaaaa aacgggcgcc cgaggcgccc gtgaatcacc aggaagaaat
      121 cttcttaaat ccgatatgcc gactctccgt gagacgtcgt gtcgagacct tcgcgttcct
      181 cttcagcggt gacacgcaga cccaccgtcc agtcggcgat cttgtaggcg atgaacgcca
      241 cgaccgctgt ccagacgatc gtcacgccca cggccttcgc ctgaatgagc aactgcgcgc
      301 cgatcccggg gtagccggtc gcgccggtga cccagtcagt gacgaggccg ggaccgccaa
      361 gagccggtga gttgaacacg ccggtgagca gggcccccag gataccgcca accgcatgca
      421 cgccaaagac gtcgagcgag tcgtcggcct tgagccagct cttcagctgg ttcacgcccc
      481 acagacacac caggcccgcg gcggtaccga tcacgaacgc gccggggatg ccgacgttgc
      541 cggcggccgg tgtgatcgcc acgagtcccg cgaccgcgcc ggaggcggcg cccagcatcg
      601 agggcttgct cttgaagatc cacgcgccgc aggtccacga cagcaccgcg caggcggtcg
      661 ccaggaacgt gttcatgaag gcgagtgcgg cggagttgtt ggcttcgagc gcggaaccgg
      721 cgttgaaccc gaaccaaccg acccacagca gcgaggcgcc gatcatggtc agggtcaggt
      781 tatgtggcgc catgtgttcc ttgccaaaac cgatgcgctt gccgatcaga taggcaccga
      841 ccaggcctgc gacgccggca ttgatgtgca ccaccgtgcc gccggcgaag tcgagcgcgc
      901 cccattgcca gagcatgccc gccttggcgt tcatcgcatc gacgacgttg gccgccgtgt
      961 aggcgtccgg acccatccag aaccacacca agtgcgcgat cggcgcgtac gagaaggtga
     1021 accacagcac catgaagatg agcaccgcgg agaacttcac gcgctcgacg aacgcgccca
     1081 ggatcagggc gcatgtgatg gccgcgaacg tcgcctggaa cgccacgaac acgagctcgg
     1141 tgatcggcgt cgccttgctg aaggtcgccc ccatggagaa cgcgccgctg gcggagtcga
     1201 acgtgccctt gaggaacaag cggtcgaacc cgccgaagta ggcgctgagc gaaccgctgc
     1261 cctcggtgaa cgcgagactg tagccgtaga cgcaccacag caccgtgatc agcgaaaaca
     1321 ccacgaacac ctgcatcagc accgacagcg cgttcttcga gcgcaccatg ccgccgtaga
     1381 acagcgccaa cgccggcaca ctcatcatca gcaccagcgc cgtggacacc agcatccacg
     1441 aggtgtcgcc cttgttcggg gtcggcgccg gagccgccgc aggtgccgga gctgctgcgg
     1501 cggctgcggc aggttctgct gcaggttttt cgtcgccaaa agcaaacaat ggcgacatga
     1561 gcagcagtac gacgctaagg gcgccgaaca gctttttcag attgaatgac gtaaacatgg
     1621 aattttctcc tgtatcaaag cgcatctttg ccggtttcac cggtgcggat acgcacgacc
     1681 tgttcgaggt catagacaaa aattttgccg tcgccgatct tgtcggtgcg cgccgacttg
     1741 gtgatcgcct cgatcacccg gtcgagttgc ttggcgtcga tcgcggcttc gatcttaact
     1801 tttggcagaa aatccacgac gtattccgcg ccgcgataca gctcggtgtg gcccttctgc
     1861 cgtccgaagc ccttgacctc ggtgacggta acgccctgca ccccgacttc cgcgagcgca
     1921 tcgcgcacgt cgtcgagctt gaagggtttg ataatggcag taacgagttt catgttgtct
     1981 cctctcgggc tcccggggcg gcaatgccgc cccggtcacc gtgactagaa cttgtagatg
     2041 gcttcaagac ccagcgaact ctgactcttc ttggtggtgc cgtccggctg attgaagctc
     2101 gactggttcg agctgtcgtg acgcacctcg ccgcgcaact cgacattctt ggccggcgtg
     2161 ttcgccaggg tcagggtgta ctccttccac ttctgcgtga cgccggtgcg gaaaccattc
     2221 ttgtcgtcga aggactcagc gcggaatgat gtacgccagg cgtcgctcag cttgtagttc
     2281 gcgtagccgg cgaggccgtt ccacttcgcg tcgcttccca cggcgatcgc atcctcttgc
     2341 ttggccttgt catagttaag gacgaagctc aggttatcgg tggcggtgaa cgtcgcgacg
     2401 atgtccagca agtcgcgccg gccagtagta gcggcaccgg cggattcatt gccgctgtaa
     2461 tacacggccg ccagcgacag gcttttggtg aggctgccgt tggcaccgag ctcgatcgtc
     2521 ttgccgagcc cttttgagcc gtcagctgcg gctgtttgct gcgactcgct ctgcacatcc
     2581 cagccgttgt tcagtcccgc gatgaaggtc cacttgccgt ccggagaaac cgcgctgccg
     2641 cccggcgtga agtaggcgcg cacaccggtg tgcgcgaacg gaatcgcata gccgaacagg
     2701 atcgagcgcg agaagttgag gttggccggg ctcttgatga cctcggcgcc ggagagcgtc
     2761 acgtacttgc caccgatcag catcaaaccg cccttggcga aatgcacata agcctgagtg
     2821 acgtcgaact catcgacggt ggtgccggcg gaggtaatta ccttggcgtc cgaaccggca
     2881 gtgagattca ccatcccgcc gaaaccctcc ggcggcagcg ccgaaagagt aagatcgagc
     2941 gcgttaatat tgaagctgcg gcgttcggta tcgaacactc gattcgctgt accgctggtg
     3001 aacgtccctg tgcctgagag ataggtatag gaggtgtcga tatagccgtt aacggatatg
     3061 cccgtggcct ccatgacctg gcccaaggtc ggcaccgccg gtttctcggg catcttttca
     3121 gcagccatca ctgtgcttac agaggcagcc gcgatgctcg ccgccagaat cgttttacag
     3181 aaattcatgt ttatctcctc aagtaacggc ttcacggtat ccacaccgct gccggatgtt
     3241 aaaaatagaa actacccagg tatttcccca aatgacttgc gcttaaaagc agtttctgtg
     3301 ccagagtatt ttctgattaa ataacagtca gttaactcat ttttgagaaa cacgcgcagg
     3361 tgtttatgca ccaacataga aaacgatttt ttcgctgtgc actatttctg tgcatcggaa
     3421 ccggaccaga cggatttata atcgcgtgcc atgatcgata gcaaaatcat tgaccagttc
     3481 gtcacggcga taacccgact cctgccggag ggtccgaagg gttttgaaaa aaacctgcgc
     3541 gccgccatgc agggggtgtt cgaccgcatg aacctggtca gccgcgagga gcttgaagta
     3601 caggaacagg tgctacagcg tacacgcgcg cggctgcagg agctggaaaa acgcatcgcc
     3661 gagctggaag cgaagctgaa aaaataaata gttccagccg catacgaacg cagatgcttt
     3721 aacccgaaaa acatatctgc gtttcaaaaa tattaataaa taactgaacc atgtcgcttg
     3781 ccgtcgttca cagccgtgcc caaattggat tggaagcacc gccggtcacg gtggaggtgc
     3841 atctggccaa cggcctgccg agcctttcca tcgtcggcct gccggaagcg gcggtgaagg
     3901 aaagcaagga ccgcgtgcgc gcggcgttgc agaacgcgcg ctttgaattt ccggcgcggc
     3961 gcatcaccgt caacctggcg ccggccgatc tgcccaagga aggtggccgt ttcgatctcc
     4021 ccatcgccat cggcattctc gtcgcttccg ggcaggtatc gaatcaccaa ctcgataaat
     4081 atgaattgat cggcgaactg gcgttgacgg gccaactgcg gccggtgctc ggtgcgctca
     4141 cggtcgcttt gcagacccgc cacaacggtc gggcgctgat cctgcccgac gccaacgccc
     4201 aagaggcagc gctggcgggc ggggccgata tccgcgccgc gaaacatttg ctcgaagtca
     4261 ctgcccattt caacagcgag aaattcctgg cgcggcatga agcggtcatg ccggcgccgg
     4321 aggtctctgt cattccggat ttgcgcgacg tccggggcca acatcaggca cggcgggccc
     4381 tggagatcgc cgcagccg
//
LOCUS       DBNGZE010000421         2919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1031758, whole genome shotgun sequence.
ACCESSION   DBNGZE010000421 DBNGZE010000000
VERSION     DBNGZE010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1031758"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /gene="fdhE"
                     /locus_tag="AC16FV_10800"
     CDS             complement(<1..226)
                     /gene="fdhE"
                     /locus_tag="AC16FV_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016131.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE
                     domain-containing protein"
                     /protein_id="HIR2850576.1"
                     /translation="MTATVIQPGIIEAPAGEIPFLILPERNAFQARAERLSALAEGHA
                     LADYLQFLAALARAQHESISLFRHVPLPDAQ"
     gene            complement(223..852)
                     /locus_tag="AC16FV_10805"
     CDS             complement(223..852)
                     /locus_tag="AC16FV_10805"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR2850577.1"
                     /translation="MNDDNLIERYKPEDRANHWLTAITLILLALSGLALFHPAFFWLT
                     NLFGGGTWTRILHPFIGVVLVVSFWFLAQRFRAYNRITDADREWMRHIRDVLAKREQN
                     LPEVGRYNAGQKYLFWIMVATIALLLASGIVLWQPWFAPLFPVGLTRVAALIHALAAF
                     VLIATTFAHIYAAIWVKGSISAMTCGSVSRAWAKQHHPGWYREVTKGTK"
     gene            complement(874..1755)
                     /gene="fdxH"
                     /locus_tag="AC16FV_10810"
     CDS             complement(874..1755)
                     /gene="fdxH"
                     /locus_tag="AC16FV_10810"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015944 - formate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HIR2850578.1"
                     /translation="MALQSLDIERRSATTTPSPGVRETQKVAKLIDISKCIGCKACQV
                     ACMEWNDLRDEIGTNRGIYDNPIDLTDKSWTVMRFSEVEVEKGKLEWLIRKDGCMHCA
                     DPGCLKACPAPGAIVQYSNGIVDFHEENCIGCGYCITGCPFNIPRLSKKDSKAYKCTL
                     CSDRVSVGLEPACVKTCPTGALVFGSKDDMIQHAEERIVDLKSRGFQNAGLYDPPGVG
                     GTHVMYVLQHADMPEIYHGLPKDPQISPMVALWKGALKPILSFGLGLVALAGFFHYVT
                     VGPNETKEDANENRHGR"
     gene            complement(1765..>2919)
                     /locus_tag="AC16FV_10815"
     CDS             complement(1765..>2919)
                     /locus_tag="AC16FV_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003162934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR2850579.1"
                     /translation="KAAEPPGEAKSDAEIMAGIFLKLRELYRKEGGAFPDPILNLTWG
                     YKIPHAPSPEEIAKEFSGKALADIPDPKEKGKVLRKAGEQLDGFAQLQDDGTTACGCW
                     IFSGAWSEKGNLMARRDNSDPSGLGNTLNWAFAWPANRRILYNRASCDPSGKPWDPKR
                     KLIAWDGAKWAGVDVPDMKPDAAPKDGMNPFIMNPEGVARFFVVDKMADGPFPEHYEP
                     FESPLDVNPLHPKNPKAKSSPAARVFKGDLEQFGDAKEFPYVATTYRLTEHFHFWTKH
                     TKLCAILQPEQFVEIGEDLAQEKKIASGDHVKVRSSRGFIKAVAVVTKRIRTLQVAGK
                     KVYTVGIPIHWGFQGVAKNGYITNTLTPFVGDANVNTPEFKAFLVDIEKA"
BASE COUNT          497 a          921 c          930 g          571 t
ORIGIN      
        1 gttgcgcgtc cggcagcggg acgtggcgga acagcgaaat cgattcatgc tgcgcgcgcg
       61 ccagcgctgc caggaactgg aggtaatccg ccagggcgtg gccttcggcc agggcgctca
      121 ggcgctcggc gcgcgcctga aaggcgttgc gttccggcag gatcagaaaa ggtatctcac
      181 ccgccggcgc ctctatgatt ccaggttgga taacggttgc ggtcatttcg tgcccttggt
      241 cacctcgcga taccagccgg gatgatgctg tttggcccag gcgcgcgaca cgctgccaca
      301 cgtcatggcg ctgatcgaac ccttgaccca gatcgcggcg tagatgtgcg cgaaggtggt
      361 ggcgatgagc acgaatgccg cgagcgcatg gatgagcgcc gccacgcgcg tgaggccgac
      421 cgggaacagc ggcgcgaacc acggctgcca caggacgatg ccgctcgcca ggagcagcgc
      481 aatcgtcgcc accatgatcc agaacaggta tttctgcccg gcgttgtagc gcccgacctc
      541 gggcaggttc tgctcccgct tggccagcac gtcgcggata tggcgcatcc attcgcggtc
      601 ggcgtcggtg atgcggttgt acgcgcggaa gcgttgcgcc aggaaccaga acgagaccac
      661 gagcaccacg ccgatgaacg gatgcaggat gcgcgtccac gtcccgccgc cgaagagatt
      721 ggtgagccag aaaaacgccg ggtggaacag cgccagtccc gaaagcgcga gcaggatcag
      781 ggtaatcgcc gtcagccagt ggttggcgcg gtcctccggt ttgtaacgct cgatcagatt
      841 gtcgtcgttc atggagcctc cttcggcggc gcatcagcgc ccgtgccggt tctcgttcgc
      901 atcctctttc gtttcattcg gacccaccgt cacgtagtgg aagaaacccg cgagcgcgac
      961 caagccaagg ccgaaggaca ggataggctt gagcgcgcct ttccacagcg ccaccatagg
     1021 actgatttgc ggatccttgg gcagaccgtg gtagatctcg ggcatgtccg cgtgctgcaa
     1081 cacgtacatc acgtgggtgc cgccgacgcc cggcggatcg tacaggccgg cgttctggaa
     1141 gccgcgcgac ttgaggtcca cgatgcgctc ctcggcgtgc tggatcatgt cgtccttgct
     1201 gccgaatacg agcgcgccgg tggggcaggt cttgacgcag gccggttcca gtcccacgga
     1261 tacacgatca gaacagagcg tgcacttgta cgccttgctg tccttcttcg agagccgcgg
     1321 aatgttgaac ggacagccgg taatgcagta gccgcagccg atgcagttct cctcgtggaa
     1381 atccacgatg ccgttcgagt actgaacgat cgcgcccggc gcgggacagg ccttgaggca
     1441 gccgggatcg gcgcagtgca tgcagccgtc cttgcggatg agccattcga gtttgccctt
     1501 ctcgacctcc acctcggaga agcgcatcac ggtccatgac ttgtcggtga ggtcgatcgg
     1561 gttgtcgtaa atgccgcgat tggtgccgat ctcatcgcgc aggtcgttcc actccatgca
     1621 cgccacctgg caggccttgc agccgatgca cttggagatg tcgatgagct tcgcgacctt
     1681 ttgcgtttcg cgcacccccg gcgacggcgt ggtggtagcg gagcggcgtt cgatgtcgag
     1741 tgattgcagt gccatatcgc ctccttacgc cttctcgatg tctaccagga acgccttgaa
     1801 ctccggcgtg ttgacgttgg cgtcgcccac gaacggcgtc aacgtgttgg tgatgtagcc
     1861 attcttggcc acgccctgga aaccccagtg gatcgggatg cccacggtgt acaccttctt
     1921 gccggcgact tgcagcgtcc ggatgcgctt cgtcaccacc gccaccgcct tgatgaaccc
     1981 gcggctcgat cgcaccttca cgtggtcacc gctcgcgatc tttttctcct gggcgaggtc
     2041 ctcgccgatc tccacgaact gttccggctg caggatcgcg cagagcttcg tgtgcttcgt
     2101 ccagaagtga aaatgctcgg tgagccggta ggtggtcgcc acgtacggaa actccttggc
     2161 gtcgccgaat tgctcgaggt cgcccttgaa cacgcgtgcc gccggactgc tcttggcctt
     2221 cgggttcttg ggatgcaatg gattcacatc gagcggcgac tcgaacggtt cgtagtgctc
     2281 ggggaacggc ccgtccgcca ttttgtccac gacgaagaaa cgcgccacgc cttccgggtt
     2341 catgatgaac gggttcatgc cgtctttcgg cgcggcatcc ggtttcatgt ccggcacgtc
     2401 cacgccggcc cacttggcgc cgtcccaggc gatcagcttg cgtttcgggt cccacggctt
     2461 gccggaggga tcgcaggaag cgcggttgta gagaatgcgg cggttggccg gccacgcgaa
     2521 cgcccagttg agcgtgttgc cgagcccgct cgggtcggaa ttgtcgcggc gcgccatgag
     2581 gttgcccttc tccgaccacg cgccggaaaa gatccagcag ccgcaggcgg tggtgccgtc
     2641 atcctgtagc tgggcaaaac catccagttg ctcgccggcc ttgcgcagca ccttgccttt
     2701 ctccttcgga tcggggatgt cagccagcgc cttgccgctg aactccttcg cgatctcctc
     2761 gggcgacggg gcatgcggaa tcttgtagcc ccacgtgaga ttcagaatcg gatccgggaa
     2821 tgcaccgcct tccttccggt agagttcacg caacttcagg aagatgccgg ccatgatctc
     2881 ggcatcggac ttggcctcgc ccggcggctc cgcggcctt
//
LOCUS       DBNGZE010000422        15542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1095762, whole genome shotgun sequence.
ACCESSION   DBNGZE010000422 DBNGZE010000000
VERSION     DBNGZE010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 15542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15542
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1095762"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16FV_10820"
     CDS             complement(<1..299)
                     /locus_tag="AC16FV_10820"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205593.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR2850580.1"
                     /translation="MTILGSTGSIGINTLDVLARHSGQYRVVALTANIHVDRLFEQCQ
                     RFLPRTAVMLDPRAATELKDKIRQAGLDTEVLSGVEGLNAVATHPQSRIVVAAIVG"
     gene            complement(363..1190)
                     /locus_tag="AC16FV_10825"
     CDS             complement(363..1190)
                     /locus_tag="AC16FV_10825"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR2850581.1"
                     /translation="MLKQRILTAIVLVPLLLAALFYLSISWIALIFGVFVAAAAWEWA
                     GLSGLRSAGARIAYVVCLVLFGVLGMNVIPLRQELIVSLLAAAVLWWLWALIELISRP
                     DARQGMFATLPGRIVGGFLVLVPLWIASVYLLAVDDARPRALLFLFVLVWVADTAAYF
                     TGSVMGRTKLAPHISPGKTVEGVVGGVLGVVLLAWLCGTMLWKFEGTWLAMWTGLAAV
                     TALFSVVGDLTESKLKRIAGVKDSGKLLPGHGGALDRIDAMTAAAPVFTLGAIFLLK"
     gene            complement(1183..1959)
                     /locus_tag="AC16FV_10830"
     CDS             complement(1183..1959)
                     /locus_tag="AC16FV_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR2850582.1"
                     /translation="MDKTGNNDTAAKLAAQSDIPRHVAVIMDGNGRWAKSRGLPRIAG
                     HRKGVERVRDMVSACAEKEIKHLTLFAFSSENWRRPAQEVQLLMDLFLSALENEIKKL
                     HKSNVRFRVIGDIGRFGEKIASRIRQAEMLTRDNQALTLTIAANYGGRWDIAQACAQL
                     AQRAARGEIDPTTITEESIEPFLSLAGVPEPDLFIRTGGEQRISNFLLWQLAYTEMYF
                     TPVLWPDFDHAQFEQALASYAGRQRRFGLTGDQIEAARHA"
     gene            complement(1984..2550)
                     /gene="frr"
                     /locus_tag="AC16FV_10835"
     CDS             complement(1984..2550)
                     /gene="frr"
                     /locus_tag="AC16FV_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR2850583.1"
                     /translation="MTQSVESIKKDAETRMAKSVETLKNDLGKIRTGRAHPGLLENVR
                     VDYYGNKTPLNQVANVSAADARTLTVTPYDKSQAAAIEKAIRESDLGLNPMSSGGVIR
                     VPLPALTEERRKELSKHARAEGENAKVAIRNVRRDSNQHLKDALKKKLITEDDDKRAE
                     EAVQKFTDRFIGEVDKLVAAKEKDIMQV"
     gene            complement(2564..3295)
                     /gene="pyrH"
                     /locus_tag="AC16FV_10840"
     CDS             complement(2564..3295)
                     /gene="pyrH"
                     /locus_tag="AC16FV_10840"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR2850584.1"
                     /translation="MTSASGVPAYRRVLLKLSGEALMGSDAYGINREVLARVAAEIKS
                     VVATGVQLAIVIGGGNIFRGIAPSAQGMDRASADYMGMLATVINSLAMQDALEKLDVP
                     VRVQSALRVDQVVEPYVRRRAIRHLEQGRVVIFAAGTGNPFFTTDTAATLRGMEIGAD
                     VVLKATKVDGVYDRDPVKHPDARRFETISFDEVLEKRLGVMDAASIALCRDQKMHLRV
                     FSIHKAGNLMRVCRGEDVGTLVTGA"
     gene            complement(3299..4186)
                     /gene="tsf"
                     /locus_tag="AC16FV_10845"
     CDS             complement(3299..4186)
                     /gene="tsf"
                     /locus_tag="AC16FV_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR2850585.1"
                     /translation="MSISAQQVKELRERTGLGMMECKAALTETNGDMEAAADLLRKRA
                     GAKVEKKAGRIAAEGTIGVYASPDRKTAAMVEVNCETDFVAKGGEFEAFAAASAARVA
                     RGQPADVDALYKLPIQDGAPTSIAQAREGLVMKLGENINVRRFERLTTDKGHIGSYVH
                     GRKIGVLVVMEGGDEALAKDIAMHVAASRPEYVSKDQVPAAAVAKEKEIFAEQAKASG
                     KPADIVEKMVMGRVNKYLNEITLLGQPFVKDPDSTVEKLLKSKGATVKGFIRYEVGEG
                     LEKRSDNFAAEVMATVKGG"
     gene            complement(4273..5199)
                     /gene="rpsB"
                     /locus_tag="AC16FV_10850"
     CDS             complement(4273..5199)
                     /gene="rpsB"
                     /locus_tag="AC16FV_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR2850586.1"
                     /translation="MSNVTMRQMLEAGVHFGHQTRFWHPKMRPYIFGERNRIHIINLE
                     KTLPLFNEAMGYLTKLATNGGTVLFVGTKRQATDIIGEEAARCGSPYVNHRWLGGMLT
                     NYRTVKKSIERLKMLESILADETSSQKLSKKELLTLDRERAKLEKSLGGIKEMSSLPD
                     AVFVVDVGHEYIAVSEAKKLNIPVVGVVDSNCKPDGVDYVIPGNDDAIRAIQLYARAA
                     ADAILVGREQHQAAIVSGDDEFIEVKEEPAKVTVVARKKPTMVPTEAAPGDEAADEGA
                     AKPAVKPKRTVRAKPKSGGRAKKEALPGTDEE"
     gene            5446..6213
                     /gene="map"
                     /locus_tag="AC16FV_10855"
     CDS             5446..6213
                     /gene="map"
                     /locus_tag="AC16FV_10855"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR2850587.1"
                     /translation="MPVTIKTPAEIEKMRVAGRLAAEVLRMIRPHVQPGITTGELDRL
                     CHDYIVNVQQAIPAPLNYKGFPRSICTSVNHQVCHGIPGDKTLKKGDIVNIDVTVIKD
                     GYHGDTSKMFFVGEPSVQAKRLAQVTHECMLKGIEMVRPGTRLGDIGHAIQQHAEAHG
                     FSVVREYCGHGIGREFHEDPQVLHYGHPGTGMALEAGMTFTVEPMINAGKKEVKLLPD
                     NWTVVTRDHSLSAQWEHTVLVTPTGYEILTLVPGDNP"
     gene            6411..9101
                     /gene="glnD"
                     /locus_tag="AC16FV_10860"
     CDS             6411..9101
                     /gene="glnD"
                     /locus_tag="AC16FV_10860"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR2850588.1"
                     /translation="MASARESLFNSREFDQSLAGTDKPLPLFRAALQTGRKNLKKRFF
                     EHSGAMLLVTAHAKLIDQLIVRAWNRHLPLLPPGFRIALVAVGGYGRGELHPASDIDI
                     MVLLEKDEPAKTQTFIETLIRFFWDMGLEVGHSVRTLKDCVREAKSDITVATNLMESR
                     LLDGDEKLFQKMRALTHASKIWPSRKFFAAKHQEQIQRHHHFHDTAYNLEPNIKDGPG
                     GLRDIHMISWVTQRHFDTASLHDLVGRGFLSEEEYHTLVRGRNFLWHVRAGLHYLAGR
                     REDRLLFDHQRELAKHFGYVDRPGFLAVEQFMKRYYRTVKELSLLNEILLQHFEEATL
                     AHGRVKIRPINRRFQSHHGFLEVTHARVFERSPFALLEMFLLMQQHPRLKGVRGGTIR
                     LVRANLHRIDNKFRKDLACRSLFMEIMRQPRGITHELRRMNAYGVLGAYLPAFGRIVG
                     QMQHDLFHVYTVDAHSLMVLRNLRRFTLPEFRNEFPLASELIQKLVKPERLYLGGLFH
                     DIAKGRGGDHSELGEKETEAFCRLHNLSEYDTRFICWLVRHHLLMSATAQREDISDPD
                     VVLRFAQRVGDQEHLDNLYLLTVADMRGTSPSVWNAWKGRLLSQLHSATSRLLLRGIA
                     QPIDMDEHVADLRRSALDELRPSGIPEQTVMRFWQDLDADYFLPYDIESLAWHAQVLA
                     QAPAADLPIVATRYTPDLGGSEFLIYTPDRDDLFVIMTAGFDRLNLSIMDARIHTLRN
                     GFALDTFVVLDHTGQPVSDARALSQLQKAMRDQMLSPKPGREIQTAGLQRQLKHFPIE
                     TRVSFSPSPKGQLTIMEVTAQDRLGLLYQVALALKHCRVNLVAAKVATYGERAEDIFF
                     INNRDRLPLTDKAQLACLEAEIIRRLAPALENSKLRSVEF"
     gene            complement(9104..9814)
                     /locus_tag="AC16FV_10865"
     CDS             complement(9104..9814)
                     /locus_tag="AC16FV_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2850589.1"
                     /translation="MKLLETRGLEVAYGGIRAVKGVDLTVRSNELVCLIGANGAGKTT
                     TLKSLCGMLVPSRGEILYEGVSIAGRPSHELVRRGISLVPEGRGIFGRLTVEENLKIG
                     AYARRDHADIRTDMDRLYGLFPRLAERRAQQAGTLSGGEQQMLAISRALMSRPRLLLL
                     DEPSMGLAPMLVQKIFETIRRIAEEGMTLLLVEQNARLALEASHRGYVMESGNIVLAD
                     SAKALLGNPQVQECYLGG"
     gene            complement(9811..10614)
                     /locus_tag="AC16FV_10870"
     CDS             complement(9811..10614)
                     /locus_tag="AC16FV_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2850590.1"
                     /translation="MTPSAERKPAAHPDTLLVVSGVSKYFGGLAALQDVSLHIGAGEI
                     YGLIGPNGAGKTTLFNVLTGLYKADAGNISLRGNRISGLRPNQVAALGMARTFQNIRL
                     FANMTAIENVMVGRHLRTRAGIVGAVWRDRVTQAEEAAILQGARELLEYVGIMKQAYM
                     PAKHLAYGDQRRLEIASALATDPVLLALDEPAAGMNPSETAGLQTLLQKIRGDGVTLL
                     LIEHDVKLVMGLCDRIAVLDYGKKIADGGPADIKRDPRVIEAYLGGSPS"
     gene            complement(10598..11722)
                     /locus_tag="AC16FV_10875"
     CDS             complement(10598..11722)
                     /locus_tag="AC16FV_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR2850591.1"
                     /translation="MLKAHWNTWRHDRRAVWIAYIVLAVLLAALPFLTGEALGRGWVR
                     ILDFTLLYVMLALGLNIVVGYAGLLDLGYIAFYAVGAYVYALLASPHFGLHLPFWALL
                     PIGAGIAAIFGILLGAPTLRLRGDYLAIVTLGFGEIIRIFLNNLNAPVNITNGPQGVN
                     LIDPIAFGSFSFSQTHSLVGMSFPGIYTYYYLFLALALLIIFVSLRLQNSRIGRAWVA
                     IREDEVAAEAMGINTRNIKLLAFAMGASFAGISGGLFAGFQGFISPESFNLFESVIVL
                     CMIVLGGMGNIAGVILGAVLLTIFPEALRYIGELQNYLFGRILADPSDLRMLIFGLAL
                     VLIMIFRPAGLLPSVRRRHEFGSDASVLAQEQASLYDTQR"
     gene            complement(11736..12665)
                     /locus_tag="AC16FV_10880"
     CDS             complement(11736..12665)
                     /locus_tag="AC16FV_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2850592.1"
                     /translation="MDILTQQLINGLVLGSIYALVALGYTMVYGILELINFAHGEVTM
                     FGAMICLAVIGAFASGGSDLPGAVIVLFGLLAAIAVCMALGFVIERVAYRPLRRAPRL
                     AALITAIGVSIVLQNLAMLIWGKQYISFPPLLPLVRYEIGGAVITNLQIFIVLLSCLM
                     MVGLLALIKRTRLGRAMRATAQAQDIAGLMGINVNTIISLTFIIGAALAAVAGVMVSA
                     YYGLAHYYMGFTLGLKAFTAAVLGGIGNIAGAMLGGLLLGVIESIGAGYIGTLTGGFL
                     GSHYQDVFAFFVLILVLVFRPSGLLGERVVERT"
     gene            complement(12779..13972)
                     /locus_tag="AC16FV_10885"
     CDS             complement(12779..13972)
                     /locus_tag="AC16FV_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2850593.1"
                     /translation="MNGPRMAKWIVWTFALALGLSACGKKEETASGTTAGEMTVKIGQ
                     VSPLTGPQAHLGKDNDNGARLALDEINAAGVTLGGKKVKFVLVSEDDMADPRTATVVA
                     QKLVDQHVAGVLGHLNSGTSIPASKIYFDAGIPQISPSATAIVYTAQGFKTAFRVMTN
                     DRQQGKVLGEYAVKKLGAKRIAIIDDRTAYGQGLADEMAKAARDAGGEIVAREFTTDR
                     STDFLAILTSIKAKKPDVVFYGGMDAQGAPMVVQLHSLGIKAKFLGGDGVQTSEFLKL
                     AGARAEGVTASSPGLPIDSMPGGREFRQKFTAKYGVIQNYAPYAYDAAMTMVEAMKKA
                     DSAEPAKYLAELPRVQRAGVTGPISFDERGDIRGGAITLYQIKNNKWEVLETVMGDAG
                     AEPKK"
     gene            complement(14091..15464)
                     /locus_tag="AC16FV_10890"
     CDS             complement(14091..15464)
                     /locus_tag="AC16FV_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR2850594.1"
                     /translation="MSKTASRLLLSVFVLMSVTAFAEPVSVQHLRVWRAPDHTRIVFD
                     VSGSIEHRLFTLQDPPRIVVDMDNARLQGPLPEVDTGGPLLSAVRSGAPEEGPLRVVL
                     DLKAEVRARSFMLKPAGPYGHRLVIDLYDAKAAQEEAVAPKDEPKTTAPADRIAPAQP
                     VARERVVAIDAGHGGEDPGAMGRRYRTREKDVTLSIARELARLVAREPGMRPVLIRDG
                     DYYVGLRQRFEKARRMRADVFVSIHADAVPGQRAYGSSVYALSEKGASNAMARLLADK
                     ENASDLIGGVSLNDKDDMLAHVLLDLSHNKTMQDSLNLGEDVLAELQHVGPVHMRNVA
                     QAGFAVLKSPDIPSVLVETAFISNPSEEKKLRTPAFQQQLAGSIFRGIKRHVARLPAP
                     SPVPVQAAISAEPTREHVVRKGETLASIARQYNIHVEALRFINDLRDADLPVGLRLRI
                     PSRQGDG"
BASE COUNT         3105 a         4922 c         4500 g         3015 t
ORIGIN      
        1 ccgacaatgg ccgcgacgac aatgcgcgac tgtggatgcg tcgccaccgc gttcaatccc
       61 tccacaccgg acaacacctc ggtgtcgagt cccgcctggc gtattttatc cttcagttca
      121 gtggcggcgc gcgggtcgag catgaccgct gtgcgcggga gaaaacgctg acactgttcg
      181 aacaggcgat caacatggat attggcggtc agcgccacga cacgatactg gccggaatgg
      241 cgcgcgagca cgtcgagcgt gttgatcccg atcgagccgg tcgatcccag aatggtcaca
      301 ccgcggagat ttcgatcagg ccgatccatg gaggaagttt tacccgtgat ggtcatacat
      361 cgttacttca gcagaaagat tgcgcccagc gtgaacaccg gcgccgcggc agtcatggca
      421 tctatacggt ccaacgcgcc gccatgaccg gggagcagtt tgccactgtc cttgacgccg
      481 gcgatacgct tgagcttgct ttcagtcagg tcgccgacca ccgaaaacag cgccgtcacg
      541 gccgccaatc ccgtccacat cgccagccat gttccctcga atttccacag cattgtccca
      601 caaagccagg ccagcagcac gacgccgagc acgcctccca ccacgccttc caccgtcttg
      661 ccgggactga tatgcggcgc aagtttggtt cgacccatga ccgaaccggt aaaataggcg
      721 gcggtatcgg ccacccacac gagcacaaac aggaacaaca gcgcccgcgg tcttgcgtca
      781 tccaccgcca gcagataaac cgatgcgatc cacaggggaa ccagaaccag gaaaccgccc
      841 acgatcctgc cgggcagcgt ggcaaacatc ccctggcggg cgtccggccg gctgatgagc
      901 tcgatcagcg cccatagcca ccacaacacc gctgccgcca gcagagagac gatcagctcc
      961 tgccgcagcg gtatgacgtt catgcccagt acgccgaaca gcacaagaca gacgacgtag
     1021 gcgatcctcg cgccggcgct tcgcagaccg gacaatccgg cccactccca cgccgccgcc
     1081 gcgacaaaga cgccgaatat cagggcaatc cacgagatag agagatagaa caacgccgcc
     1141 agcagcagcg gaacgagaac gatggcggtc aggatgcgct gtttaagcat ggcgcgcggc
     1201 ttcgatctga tcaccggtca gaccgaaacg gcgctggcgt ccggcatacg aggccagcgc
     1261 ctgctcgaat tgcgcgtgat cgaaatccgg ccacaggacc ggtgtgaaat acatctcggt
     1321 gtaggcgagt tgccacaaca ggaaattgct gatgcgctgc tcgcccccgg tgcggatgaa
     1381 aagatcgggt tcgggcacgc cggccaagct gagaaagggc tcgatgctct cctccgtgat
     1441 ggtcgtgggg tcgatctcgc cgcgcgcggc gcgttgcgcc agttgcgcgc aagcctgggc
     1501 gatgtcccag cgtccgccat aattggcggc gatggtcagc gtcagcgcct gattgtcgcg
     1561 cgtaagcatt tccgcctggc ggatgcgtga agcgattttc tcgccgaaac ggccgatatc
     1621 gccgatgaca cggaaccgta cgttactctt gtgcagcttc ttgatctcgt tctccagcgc
     1681 gctcagaaac aaatccatga gcaactgtac ttcctgcgcc gggcgacgcc agttctcgct
     1741 gctgaaggca aaaagcgtga ggtgcttgat ttccttctcc gcgcaagccg acaccatgtc
     1801 gcgcacgcgt tccacgccct tgcgatgacc ggcgatgcgg ggcaatccgc gactcttggc
     1861 ccaacggccg ttgccgtcca tgatcacggc cacgtgacgc gggatgtcgg attgcgccgc
     1921 cagctttgct gcagtgtcgt tattaccggt cttgtccatt cgtataacag ttcggcagaa
     1981 aaatcatacc tgcatgatgt ccttttcctt ggctgccacc agcttgtcca cttcgccgat
     2041 gaaacggtca gtgaatttct gcaccgcctc ttccgcgcgc ttgtcgtcgt cctcggtgat
     2101 gagcttcttt ttcagggcgt ccttcagatg ctggttggaa tcgcggcgga cattgcggat
     2161 tgccaccttg gcgttctcgc cttcggcgcg cgcatgcttg gaaagttcct tgcggcgctc
     2221 ctcggtcaac gccggcaacg gcacgcggat cacgccgccc gaagacatgg gattcagccc
     2281 cagatcggat tcccgaatcg ccttttcaat cgcggcggcc tggctcttgt cgtaaggcgt
     2341 caccgtcagc gttctggcgt ccgcggccga gacgttggcc acctgattca gcggcgtctt
     2401 gttgccgtaa taatcgacgc gcacgttttc aagcaggccg ggatgagcgc gaccggtgcg
     2461 tatcttcccc agatcgtttt tcagcgtctc caccgacttc gccatgcgcg tttccgcgtc
     2521 tttcttgatg ctctcgactg attgcgtcat gcctgggctc cttttaggct cctgttacaa
     2581 gcgtgccgac atcttccccc cggcaaacac gcatcaggtt gccggcttta tgaatgctga
     2641 acacgcgcag atgcatcttc tggtcgcggc acagcgcgat ggaagccgcg tccatcacac
     2701 ccagccgctt ttccagcact tcatcgaaac tgatggtttc aaaacgcctg gcgtcgggat
     2761 gtttgaccgg atcacggtcg tacacgccgt cgaccttggt ggccttgagc acgacgtccg
     2821 cgccaatttc catgccgcgc agcgtcgccg cggtgtcggt ggtaaagaag ggattgccgg
     2881 tgcctgcggc gaaaatcacc acgcgtccct gctcgagatg acgtatggcg cggcggcgca
     2941 cgtagggttc caccacctga tcgacccgca gggccgactg cacgcgcacc ggaacatcca
     3001 gtttctccag cgcatcctgc atggccagcg aattgatcac ggtggcgagc atgcccatgt
     3061 aatcggccga ggcccgatcc atgccctggg ccgagggcgc gatgccgcgg aaaatattgc
     3121 cgccgccgat aacgatggca agctgcacgc cggtggcgac taccgacttg atctcggccg
     3181 cgacgcgcgc cagcacttcg cgattgatcc cgtaggcgtc ggaacccatc aacgcctcgc
     3241 cggaaagctt gagcagcacg cggcggtagg cgggaacccc ggatgcgctc gtcaagattc
     3301 agcccccttt gacggtcgcc atcacttcgg cggcgaaatt gtcggaccgc ttctccagac
     3361 cttcacccac ttcgtaacga atgaaaccct tcacggtcgc gcccttggac ttgagcagct
     3421 tttccaccgt gctgtccggg tccttcacga aaggctgtcc cagcagggtg atttcattga
     3481 ggtacttgtt gacccggccc atgaccattt tttccacgat gtcggccggt ttgccgctgg
     3541 ccttggcctg ctcggcgaat atttcttttt ccttggcgac ggccgcggcc ggcacctggt
     3601 ccttcgagac atattccggc cggctggcgg cgacatgcat ggcgatgtct ttggcgaggg
     3661 cttcatcgcc tccttccatc accaccagca cgccgatctt gcgtccatgg acatagctgc
     3721 cgatatggcc cttgtccgtc gtcagccgct cgaagcggcg cacgttgatg ttctcgccca
     3781 gtttcatgac cagaccctcg cgcgcttgcg cgatgctggt cggcgcgcca tcctggattg
     3841 gaagcttata gagcgcatcc acgtccgccg gctgaccgcg ggccacgcgc gccgcggacg
     3901 cggcggcaaa ggcctcgaat tcaccgccct tggcgacgaa atcggtttca caattcacct
     3961 cgaccatggc ggcggtcttg cgatcggggc tggcatacac accgatcgtg ccttcggcgg
     4021 cgatacgacc ggcttttttc tcgaccttgg cgcccgcgcg cttgcgcagc agatcggctg
     4081 cagcctccat gtcgccattc gtttccgtga gcgcggcttt gcattccatc atgcccagtc
     4141 cggtgcgctc gcgcagttct ttcacttgtt gtgctgaaat gctcatacct gatcctcgtc
     4201 ttgccttgcc ggcggcaaac accgcctaca tagaatcaag ggggcaatgc ccccttgatg
     4261 ttgtcacgtc tgttattctt cgtccgttcc gggaagcgcc tcttttttcg cgcgcccgcc
     4321 gcttttgggc ttggcgcgca cggtgcgctt gggcttgacc gccggttttg cggcgccttc
     4381 atccgccgct tcgtccccgg gcgcggcctc ggtcggtacc atggtcggct ttttcctggc
     4441 aacgaccgtg accttggctg gctcttcctt cacttcgatg aactcatcgt cgcccgacac
     4501 aatcgcagcc tgatgctgct cgcggccgac aagaatggcg tccgccgcgg cgcgcgcata
     4561 gagctggatg gcgcggatgg cgtcgtcatt gcccggaatg acgtaatcaa caccatcggg
     4621 cttgcagttg gaatcgacca cgcccacaac gggaatattc agcttcttcg cctcggaaac
     4681 cgcgatgtac tcatggccta cgtcaacgac aaacacagcg tcaggcaggc tggacatctc
     4741 cttgatgccg cccaggctct tttcgagctt ggcacgttca cgatcgaggg tgagcagctc
     4801 tttcttggaa agtttctggc tgctggtttc atccgccaga atggattcaa gcattttcag
     4861 gcgctcaatg gatttcttca cagtgcggta attggtcagc atcccgccca gccagcgatg
     4921 attcacatag gggctgccgc agcgggccgc ctcttcgccg atgatgtcgg ttgcctgacg
     4981 cttggtaccg acaaacagga ccgtgccgcc gttggtggca agcttggtca ggtaacccat
     5041 cgcctcattg aacagcggca gggtcttttc caggttgata atgtggatac ggttgcgctc
     5101 gccgaaaata taggggcgca tcttggggtg ccagaaccgc gtctggtggc cgaaatgcac
     5161 gccggcctcc agcatctggc gcatggtgac gttgctcatg atatctcctt cgggtttttc
     5221 cgccgcggat cccatgcatc aatccagcca atcccgtggg gacgaccgga cacccggatg
     5281 cctgtgacga tgccgcgtgt gggtttaagg gtttaacaac ttgaactgcg ggcggcttta
     5341 taccacaagc agggcgatta cgacaatgtt ttcatgggtt tggactgaaa aagggctttt
     5401 taaaaccgtt ccattcccgg acaatacggc tgtaatcgag cacttatgcc cgtcaccatc
     5461 aaaacccccg ccgaaatcga aaaaatgcgc gtcgccggcc ggctcgccgc cgaggtgctg
     5521 cgcatgatac ggccgcacgt ccagcccggg atcacgaccg gcgaactcga ccggctgtgc
     5581 cacgattaca tcgtcaacgt gcagcaggcc attccggcgc cgctcaacta caagggcttc
     5641 ccgcgctcca tctgcacctc ggtgaatcac caggtctgtc atggcattcc gggcgacaag
     5701 acactcaaga aaggcgatat cgtcaacatc gacgtcaccg ttatcaagga tggttatcac
     5761 ggcgacacca gcaaaatgtt tttcgtgggc gagccctccg ttcaggcgaa gcgcctagcg
     5821 caggtgacgc acgagtgcat gctcaagggc attgaaatgg tgcgaccggg aacgcggctc
     5881 ggggacatcg gccacgccat ccagcaacac gccgaggcac acggtttctc ggtggtgcgt
     5941 gaatattgcg gccacggcat cgggcgcgaa ttccacgaag acccgcaggt gctgcactac
     6001 ggccatccgg gaacggggat ggcgctggaa gccggcatga ccttcaccgt cgaacccatg
     6061 atcaatgccg gcaagaagga agtaaaactg ctgcctgaca actggaccgt ggtcacgcgc
     6121 gatcattcgc tctctgcgca atgggaacat accgtgctgg tgacaccgac gggttatgaa
     6181 atccttacgc tcgtcccggg cgacaatccg tgattttgaa aaatattttc aaccgccagg
     6241 acgctggggc gagccggaaa cagacgaaga cacttaacgt gtcttcgtcc cggcgagcgc
     6301 ccgcacacgg atgtgcgggc tgggggatat cggagcctac aacgttgcgc cagggatggc
     6361 tgggaccggc accctggcgg ttcagaatca tatttctttt ttgagctaat atggccagcg
     6421 ccagggaaag tctgttcaac agtcgcgaat tcgaccaatc actggccgga accgacaagc
     6481 cccttcccct cttccgcgcc gcgttacaga ccgggcgcaa aaatctcaaa aaacgttttt
     6541 ttgaacacag cggcgccatg ctgctggtaa cggcgcacgc caagctgatc gatcagctga
     6601 tcgtgcgtgc atggaaccgg catttgccgc tgctgccgcc cggttttcgt atcgccctgg
     6661 tggccgtggg cggctacggg cgcggcgagc tgcatcccgc ctccgatatc gacattatgg
     6721 tattgctcga aaaggacgag ccggccaaaa cacaaacctt cattgagacc ctgatccgtt
     6781 tcttctggga catgggactc gaagtcggtc acagcgtgcg cacgctgaag gattgcgtgc
     6841 gcgaggcgaa aagcgacatc actgtggcca ccaacctcat ggaatcgcgc ctgctcgatg
     6901 gcgacgagaa gttgttccag aaaatgcgcg cgctcacgca tgcctcaaaa atatggccga
     6961 gccgcaaatt tttcgccgcc aagcaccagg aacagataca gcggcatcat cacttccacg
     7021 acaccgccta caacctggaa ccgaacatca aggacggtcc gggcgggctg cgtgacatcc
     7081 acatgatctc gtgggtgacg cagcggcact tcgataccgc ctcgttgcac gatctggtgg
     7141 gacgcggctt cctgagcgag gaggaatatc acacgctggt acgcggcaga aatttcctgt
     7201 ggcacgtgcg cgccgggctg cactacctcg ccggccggcg cgaagaccgg ctactgttcg
     7261 atcaccaacg cgagctggcc aaacacttcg gttacgtgga tcgcccggga ttcctcgccg
     7321 tcgagcagtt catgaaacgc tactaccgga ccgtcaagga actgtcgctg ctcaatgaaa
     7381 ttttgttgca gcacttcgag gaggccaccc tcgcgcatgg acgtgtcaaa attcggccca
     7441 tcaaccgccg cttccagtcg catcacggtt ttctggaggt gacgcatgcg cgggtattcg
     7501 aacgctcgcc gtttgccttg ctggagatgt tcctgctcat gcagcaacac ccccgtctca
     7561 agggcgtgcg cggcggcacc atccgcctgg tgcgcgccaa tctccaccgc atcgacaaca
     7621 aattccgcaa ggacctcgcc tgccgcagcc tgttcatgga gatcatgcgc cagccgcgcg
     7681 gcatcacgca cgagctgcgg cgcatgaacg cctacggcgt gctgggtgcc tacctgcccg
     7741 ccttcggccg gatcgtcggt caaatgcagc atgacctgtt ccacgtgtac acggtcgatg
     7801 cccattcgct catggtgctg cgcaacctgc gccgtttcac cctgccggaa ttccgtaacg
     7861 aatttccgct ggccagtgaa ctcatccaga agctggtcaa gcccgagcgc ctgtacctgg
     7921 gtgggctgtt tcacgacatc gccaaggggc gtggcggcga tcactccgag ctcggggaaa
     7981 aagaaaccga ggccttctgc cgtctgcata acctgagcga gtacgacacg cgttttattt
     8041 gctggctggt gcgccaccac ctgctgatgt cggccaccgc gcagcgcgag gatatctccg
     8101 accccgacgt cgtgttgcgt ttcgcccagc gggtcggcga ccaggagcat ctcgacaacc
     8161 tctatctgct gacggtggcg gacatgcgtg gcaccagtcc gagcgtctgg aacgcctgga
     8221 aaggccgcct gctgtcgcag ctccacagcg ccacctcgcg cctgttgttg cgcggcatcg
     8281 cccagccgat cgacatggac gaacacgtcg ccgatttacg ccgctccgcg ctggacgaac
     8341 tgcggccctc agggattccg gaacagaccg tcatgcggtt ctggcaggac ctcgatgcgg
     8401 attatttcct gccctacgat atcgaaagcc tcgcctggca cgcccaagtc ctggcccagg
     8461 cccccgccgc cgacctgccg atcgtggcca cgcgttatac ccccgacctc ggcggcagcg
     8521 aattcctgat ttacaccccg gaccgcgacg atctgttcgt gatcatgacc gccggcttcg
     8581 accgcctgaa cctgtcgatc atggacgcgc gtatccacac gctgcgcaac ggtttcgcgc
     8641 tcgacacctt cgtggtgctg gatcacaccg gccagcctgt aagcgatgcg cgcgcattat
     8701 cacagctgca aaaagccatg cgcgaccaga tgctgagccc gaagccggga cgcgaaatcc
     8761 agacagcggg cttgcaacgc caactcaagc acttccccat cgaaacacgc gtgagcttca
     8821 gtccgtcacc gaagggacag ctcaccatca tggaagtcac ggcgcaggac cgcttgggat
     8881 tgctctatca ggtggcgctc gcgctcaaac actgccgggt gaacctggtg gcggccaagg
     8941 tggccaccta cggcgagcgc gccgaggata ttttcttcat caataaccgt gatcgcctac
     9001 cactcacgga caaggcccaa ctcgcttgtc tcgaggccga gatcattcga cgcctggcgc
     9061 ctgccttgga aaattccaaa ctccggtcag tcgagtttta atatcaaccg cccagatagc
     9121 attcctggac ttgcgggttt ccgagcagcg cctttgccga atcggcgaga acaatgtttc
     9181 cactctccat gacgtaacca cgatggctgg cttcgagagc gaggcgggca ttctgttcca
     9241 ccagcagcag cgtcatgccc tcctccgcaa tgcgtcgaat cgtctcgaaa atcttctgca
     9301 ccagcatggg cgccagaccc atgctgggtt catcgagcag caacaggcgc ggacggctca
     9361 tcagcgcacg gctgatggcc agcatctgct gctcgccgcc ggaaagcgtg ccggcctgtt
     9421 gcgcgcgccg ctcggccagc cgcgggaaaa gtccgtatag acgatccatg tcggtacgga
     9481 tatcggcatg atcgcggcgc gcgtaggcgc cgatcttcag gttctcctcc accgtcaatc
     9541 gcccgaagat gccgcgccct tccggcacca gtgatatccc acgccgtacc aattcgtggg
     9601 atggccggcc agcgatagag acgccttcat acagaatctc gccccggctt gggaccagca
     9661 tcccgcacag ggatttgagc gtggtggtct tgcccgcccc gttggcgccg atcaggcaca
     9721 cgagttcatt gctgcgcacc gtcagatcca cgcctttgac ggcgcgaata ccgccatagg
     9781 ccacttccag tccacgggtt tccagaagct tcaactggga ctccctccca gataggcctc
     9841 aatgacgcgc gggtcgcgct tgatgtcggc cggcccacca tcggcgattt tttttccgta
     9901 gtcgagcacg gcaatgcggt cgcacaaacc catgacgagc tttacatcgt gctcgatcag
     9961 cagcaaggtc acgccatcac cgcgaatttt ctgcaacagc gtctgaagcc cggcggtttc
    10021 gctgggattc atgccggccg ccggctcgtc gagcgcgagc aataccggat cggtggccag
    10081 ggcgcttgcg atttcaagac ggcgttgatc gccgtaggca agatgcttgg ccggcatgta
    10141 cgcctgtttc atgatgccga cgtattccag cagttcacgc gccccttgca gaatcgccgc
    10201 ttcttctgcc tgtgtaacgc gatcgcgcca gaccgcgccg acgatgccgg cgcgcgtgcg
    10261 cagatgccgg cccaccatga cgttttcaat cgccgtcatg ttggcgaaca ggcgtatgtt
    10321 ctgaaacgtg cgcgccatgc cgagcgcggc tacctggttc ggcctgagcc cggaaatccg
    10381 gttgccgcgc aaactgatgt ttccggcatc ggccttgtac agaccggtca gaacattgaa
    10441 cagggtcgtc ttgccggcgc cgttcggccc gatcagcccg tatatctcac cggcaccgat
    10501 atgcagcgac acatcctgca gcgccgcaag cccgccgaaa tattttgaca cgccgctgac
    10561 gacgagcaac gtatcaggat gagctgccgg cttcctttca gcgctgggtg tcatagagcg
    10621 aggcctgttc ctgggccagc acggaggcat cggaaccgaa ttcatgacgg cggcgcaccg
    10681 acggcagcaa accggccgga cggaagatca tgatcaaaac cagtgcgagt ccgaatatca
    10741 gcatacgcag atcggaagga tcggccagga tgcggccgaa gaggtaattt tgcagctcgc
    10801 cgatgtaacg caaggcttcg gggaatatgg tcagcagcac ggcgccgaga atgacgccgg
    10861 cgatattgcc catgccgccg agcacgatca tgcacaggac aatgacggac tcgaacagat
    10921 tgaagctttc cggactgatg aagccctgaa agcccgcgaa cagaccgccg ctgataccgg
    10981 cgaacgatgc gcccatggcg aaggccagca gcttgatgtt gcgcgtattg atgcccatgg
    11041 cctcggcggc cacctcgtct tcgcggatcg ccacccaggc gcggccgatg cgcgagtttt
    11101 gcaggcgcag cgaaacgaaa atgatcagaa gcgcgagtgc gagaaacaga tagtaatagg
    11161 tataaatgcc ggggaagctc atcccaacaa gactatgcgt ctgactgaat gaaaaactgc
    11221 caaaagcgat cggatcgatc aggttgacgc cctgcgggcc gttcgtgata ttcacgggcg
    11281 cgttgagatt gttgaggaat atgcggatga tctcgccgaa gccgagcgtt acgatggcca
    11341 gataatcgcc acgcaagcgc agggttggcg cacccagcag aatcccgaaa atggcggcga
    11401 tcccggcgcc gattggcaac aatgcccaga acggcagatg cagcccgaaa tgcggcgagg
    11461 ccagcaaggc atagacatag gcgccgacgg cgtaaaacgc gatatagccg agatcgagca
    11521 gcccagcata acccaccacg atattgagtc cgagcgcgag catgacatac agcagcgtga
    11581 aatcgaggat gcgcacccac ccgcgcccga gcgcctcgcc ggtcagaaac ggcaacgccg
    11641 ccagcagcac ggccagtacg atataggcta tccataccgc ccgacgatca tgccgccaag
    11701 tgttccaatg agctttcagc atatgcatgc ggtaatcagg tgcgctccac aacgcgctcg
    11761 cccagcaggc cggagggccg gaagaccaga accagaataa gcacaaagaa ggcgaagaca
    11821 tcctgatagt gactgccgag aaaaccgcct gtcagcgtgc cgatgtatcc cgcaccgatg
    11881 ctctcgatga cgcccagtaa caatccaccc aacatcgctc ccgcgatgtt gccgataccg
    11941 cccagcaccg ccgcggtaaa ggccttgagg ccgagcgtga agcccatgta gtaatgagcc
    12001 agaccataat atgcgctgac catgacgccg gcgaccgccg ccagcgccgc gccgatgatg
    12061 aacgtgagcg agataatggt gttgacgttg atgcccatca ggccggcgat gtcctgcgcc
    12121 tgcgcggtgg cgcgcatggc gcgccccagg cgcgtgcgct tgatgagcgc caacagaccc
    12181 accatcatca ggcaggaaag gagcacaatg aaaatctgca agttggtgat gacagcgccg
    12241 ccgatttcat agcgaaccag cggcagcagt ggcgggaaac tgatgtattg cttgccccag
    12301 atcagcatcg ccagattttg caggacgatt gagacaccga tggcggtaat gagcgccgcc
    12361 agtctcggtg cgcggcgtaa cgggcgatag gcgacgcgct cgatcacgaa acccaacgcc
    12421 atacagacag cgatcgcggc cagcaaaccg aacaggacga tgactgcgcc gggcaggtcg
    12481 ctaccgccgc tcgcgaaagc gccaatgaca gcgaggcaga tcatggcccc gaacatggtc
    12541 acctcgccat gcgcgaagtt gatcagctcg aggatgccgt acaccatggt gtaacccagc
    12601 gccaccaggg catagatgct tcccagaacc agaccgttga tcagctgctg ggtgaggatg
    12661 tccatctatt atgattgtgt aaccgcagat gaacgcggat aaacgcagat tagaactgtc
    12721 attggttttt gtgtttttta tctgtgttca tctgcgttta tctgcggttt catggttttt
    12781 attttttcgg ttctgcaccg gcgtcgccca tgacggtttc cagaacctcc catttgttat
    12841 ttttgatctg atacaacgtg atggcgccgc cgcggatatc gccacgctcg tcgaagctga
    12901 tgggaccggt gacaccggcg cgctgaacgc gcggcagctc ggccaggtac ttggcgggct
    12961 cggccgaatc cgccttcttc atggcctcca ccatggtcat ggcggcgtca taggcataag
    13021 gcgcgtaatt ctggataacg ccgtatttcg cggtgaattt ttgccggaat tccctgcctc
    13081 ccggcatgct gtcgattggc aagccgggcg atgaggccgt cacaccttcg gcgcgggcgc
    13141 cggcgagctt gagaaattcc gaggtttgca cgccgtcgcc accgagaaat ttggccttga
    13201 tgccgagcga atgcaattgc accaccatcg gcgcgccctg cgcgtccatg cctccgtaaa
    13261 aaaccacatc gggcttcttg gccttgatcg aggtgagaat ggcgaggaaa tccgtggagc
    13321 gatcggtggt gaactcgcgc gccacgatct cgccacccgc gtcccgcgct gccttggcca
    13381 tctcgtccgc gagtccctga ccgtaggccg tacggtcgtc gatgatcgca atgcgcttgg
    13441 cgccgagctt ctttaccgcg tactcaccca atacctttcc ctgctggcga tcgttggtca
    13501 tgacgcggaa ggcggtcttg aatccctggg cggtatagac aatagcagtg gcggagggag
    13561 aaatctgggg aatgccggcg tcaaaataga tcttcgaggc gggaatgctg gtgccggagt
    13621 tcagatgacc gagcacgccg gccacatgct ggtccaccag cttctgcgcc accacggtgg
    13681 cggtccgcgg atcggccata tcatcctcgc tgaccagcac gaacttcacc ttcttgccgc
    13741 ccagtgtgac gccggcggcg ttgatttcat cgagtgcaag gcgcgcgccg ttatcgttgt
    13801 ccttgcccag atgggcctgc ggtccggtga gcggcgagac ctgcccgatc ttcaccgtca
    13861 tttcgccggc tgtcgtgccg ctggcggttt cttccttctt gccgcaggcg ctcagtccaa
    13921 gcgccagcgc gaatgtccag actatccatt tcgccatacg cggtccgttc atgaaatgtc
    13981 tccttagata tttttctgga agcggcattg acgtaaatct tagcgcaaaa accccgcccg
    14041 gacccaataa atagcgacaa gtccggagtg gcgagacaac gaatttaagg ttaaccgtcc
    14101 ccctgtctgg acggaatgcg aagccgcaga ccaaccggga gatccgcgtc ccgcaggtca
    14161 ttgataaaac gcagggcttc cacgtgaata ttgtactgcc gcgcaatcga ggccagtgtc
    14221 tcgcccttgc gcacgacgtg ctcacgcgtc ggttcggcgg aaatcgccgc ctgcaccgga
    14281 accggcgatg gcgccggaag gcgtgccaca tggcgcttga tgccacggaa aatgctgccg
    14341 gccagttgct gctggaatgc cggtgtgcgc aatttcttct cttccgaagg gttggagatg
    14401 aacgcggtct cgaccagcac cgacgggatg tcgggcgact tgagtacggc gaacccggcc
    14461 tgggcgacgt tgcgcatgtg taccggaccc acgtgctgca actccgcgag cacatcctcg
    14521 cccaggttca agctgtcctg catggtcttg ttgtgagaaa ggtcgagcag aacatgcgcc
    14581 agcatgtcgt ccttgtcatt caggctgaca ccaccgatca gatcggacgc gttttccttg
    14641 tcggccagca gacgcgccat ggcgttgctc gcacccttct ccgacagcgc atagaccgat
    14701 gagccataag cacgctgacc ggggacggca tccgcatgga tagacacgaa cacgtccgcg
    14761 cgcattctgc gcgccttttc gaaacgctgg cgcagaccga cataataatc tccgtcgcgg
    14821 atcagcaccg gccgcatccc aggctcccgc gccacgagcc tggccagctc gcgcgcaatg
    14881 gagagcgtca cgtccttttc gcgcgtgcga taacgccgcc ccatcgcgcc cgggtcttca
    14941 ccgccgtgcc cggcgtcaat cgccaccacc cgttcgcgcg ccaccggttg cgccggtgca
    15001 atgcgatccg ccggtgcggt tgttttgggt tcgtctttcg gcgcgactgc ttcttcctgc
    15061 gccgccttcg cgtcgtagag atcgatcacc agacggtgcc cgtaaggtcc ggccggcttc
    15121 aacatgaaac tccgcgcgcg cacttcggcc ttgagatcca gcacgacgcg caagggccct
    15181 tcctccggcg caccactgcg cacggcggag agcagcggac cgcccgtgtc cacctccggc
    15241 agaggtcctt gcaggcgcgc gttgtccatg tccaccacga tgcgcggcgg atcctgcagg
    15301 gtaaaaagac gatgctcgat cgaaccgctg acatcgaaca cgatgcgagt atggtccggt
    15361 gcgcgccaca cgcgcagatg ttgcacggag acgggctccg cgaaagccgt cacggacatc
    15421 agcacgaaaa cagagagcag caggcgcgac gctgttttgc tcatgtcagc gccttcagca
    15481 aaacatcgcc ctggggtgtg tgggtcgtga tccgtaccat gcgtcctgcc tcggtcggtt
    15541 ca
//
LOCUS       DBNGZE010000423         8868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1792347, whole genome shotgun sequence.
ACCESSION   DBNGZE010000423 DBNGZE010000000
VERSION     DBNGZE010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1792347"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC16FV_10895"
     CDS             <1..540
                     /locus_tag="AC16FV_10895"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015551361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR2850595.1"
                     /translation="REGVCGSDGMNINGRNGLACITPLKDLKQPIELKPLPGVPVVRD
                     LIVDLSQFYSAYRTVKPWVINHDPEPEIERLQSPEDRDKLDGLYECVLCACCTTACPS
                     FWWNPDKFLGPAALLQSWRFLADSRDQATGERLDGLEDPYKLFRCHTIMNCTEVCPKG
                     LNPAKAIGHIKMMLAKRVL"
     gene            537..848
                     /locus_tag="AC16FV_10900"
     CDS             537..848
                     /locus_tag="AC16FV_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR2850596.1"
                     /translation="MMGESPSPSSPSLLPPREKGASRSAQDARQFDRLKWRCRRGLLE
                     LDVVLKDFLDRRYSNLTPAEQEAFEKLLTTSDETLLAYVQGKQNPPEKELMQIIAKLR
                     N"
     gene            916..1272
                     /locus_tag="AC16FV_10905"
     CDS             916..1272
                     /locus_tag="AC16FV_10905"
                     /inference="COORDINATES: protein motif:HMM:NF018908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850597.1"
                     /translation="MALVTAVPVIWSVRLGLWGVLGWSLYYSLVTHAWRSSALAITAI
                     EMDNEEQVSVRLAGSEVWQTAQIASRFVHPWLTLMSLRLEGRRWPVSLVIAADAVEAE
                     PFRRWRVALKLQVVPV"
     gene            complement(1226..2866)
                     /gene="nadB"
                     /locus_tag="AC16FV_10910"
     CDS             complement(1226..2866)
                     /gene="nadB"
                     /locus_tag="AC16FV_10910"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR2850598.1"
                     /translation="MTTLQSSDVLIIGGGIAGLSLALRIADKARVTILAKAALTEGSS
                     LYAQGGVAAVLNGEEDSFESHIQDTLDAGAGLCHRDAVEFVVREGPTSIRWLIERGVP
                     FSREEHPHNGLEYHLTREGGHSHRRVIHAADATGRAMETTLEGLVRRHPNITLHENHI
                     AIDLITSQKLGRPGPNRSLGVYALDKADGHIRAYSARFVVLATGGACKAYLYTSNPDT
                     STGDGIAMAWRAGCRVANMEFVQFHPTCLYHPRAKSFLISEAVRGEGGKLTLPNGKPF
                     MQHHDPRGELASRDIVARAIDYEMKRGGLDYVHLDISHKPADFIKSHFPTIYERCKEY
                     GYDITREPIPVVPAAHYTCGGVVTDLSARTDLDRLYAVGECSFTGLHGANRLASNSLL
                     ECLVFAKAAAEDILKQLPTAGPIPEGLPAWDESRVTDPDEQVVVSHNWDELRRGMWSY
                     VGIVRTTKRLQRAQHRVEMLKEEVREFYSNFKVTNDLIELRNLLLVSELIIRSALARK
                     ESRGLHFTRDYPQPVSGTPQDTVLTPAPPEVSGRRANA"
     gene            3077..3655
                     /gene="rpoE"
                     /locus_tag="AC16FV_10915"
     CDS             3077..3655
                     /gene="rpoE"
                     /locus_tag="AC16FV_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR2850599.1"
                     /translation="MSERSIDQKLVVRVQKGDKTAFDLLVRKYQHKIAKLISRYVRDR
                     REVEDVTQEALIKAYRAINGFRGESAFYTWLYRIAVNTAKNYLESQGRRPPGTDMEIE
                     TAELADGGSSLREQATPERQMLTDEIANTVHRVIEGLPEDLRTAITLREIEGMSYEEI
                     AQVMDCPIGTVRSRIFRAREAVDQELRPLLEK"
     gene            3658..4215
                     /locus_tag="AC16FV_10920"
     CDS             3658..4215
                     /locus_tag="AC16FV_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR2850600.1"
                     /translation="MSNTDEMKEKLSALVDNELDDVNERRVMAVLEKDADLRRTWERY
                     HLVRSVLHQELDAFVPPDMAEHVAARISMEPANTSAFRRQKITRYAGMFAIAASVAAI
                     AVTSVQWLSRPISAPVTPVASVQPAPDNFIRAGATRWDMKQNEPETESALNAFLVEHN
                     EFASSSGIGGMMPYVRVVGYDNSAK"
     gene            4212..5192
                     /locus_tag="AC16FV_10925"
     CDS             4212..5192
                     /locus_tag="AC16FV_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR2850601.1"
                     /translation="MNFRKLTPYFFFPACLVFSTAVLAGDAAHDWLMKINQAVRMLDY
                     EGVFVYQHENQLEAMRIIHKVENGAARERLVSLNGAPREVIRDEREVTCYWPDKNSVM
                     VEYRKADSKAFPAILPERIQDLDDYYAIRLGNMERITGRSAQLVIVKPVDQYRYGYHL
                     WADKETGLLLKADLLDIQGRILEQFMFTQINIGAKIPASALAPGVAGKGMVWHREDED
                     RKTITAKAGWTATQLPKGFRLSAHILRQTLMRKRPMEHLVYTDGLAAVSVFVVKRDED
                     SKPFMLGPSRMGAVNALGSRVDDYQITTVGEVPAETIALIGGSVTPVANQ"
     gene            5276..6679
                     /locus_tag="AC16FV_10930"
     CDS             5276..6679
                     /locus_tag="AC16FV_10930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR2850602.1"
                     /translation="MTARLRYSIFLVLFFSAPLAAAQRALPDFSQLVTTRGTAVVNIS
                     VTRDLKSVGFSTDTVAPDDDSARDFTRRYSGENTAPAEQNSLGSGFIISPDGYIITCA
                     HVTENAHEILVRLNDRREFSARLVGSDRRSDIALLKIESTGLPTIVIGDASRLQVGEW
                     VLAIGSPFGFDNSATAGIVSAKGRTLPNENYVSFIQTDVPINPGNSGGPLFNLKGEVV
                     GVNSQIYSGTGSFMGVSFAIPIDAAMTIGAQLKSEGRVRRGWLGVSLQDVSLALANAY
                     GLNKPRGALVADVLPKSPAAKSDLRPGDIVIEYESKPIDRSSDLPTLVGLSMPGTHAR
                     FRVFRRDQGMQTVVVTLGELKEESSPRPPLGKDRMEGQLRFGLSLGDITERQRREFDI
                     DHGASVNDVDEGRARDAGLRPGDVILEVDGKRVTDVAGFHRLLAHVRPGRPAVLRVRR
                     GMATLFLALDNINRPAP"
     gene            6896..8698
                     /gene="lepA"
                     /locus_tag="AC16FV_10935"
     CDS             6896..8698
                     /gene="lepA"
                     /locus_tag="AC16FV_10935"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR2850603.1"
                     /translation="MSWDQEHIRNFSIIAHIDHGKSTLADRFIQTCGALEAREMQEQV
                     LDSMDLERERGITIKAQSVALDYKARDGQTYRLNMIDTPGHVDFSYEVSRSLTACEGA
                     LLVVDASHGVQAQTVANCYTAAELDLEILPVLNKIDLPSADPARAIAEIEDIIGIDAG
                     DAIRASAKTGEGVTDILEAIVRRIPVPKGRAEAPLKALIIDSWFDNYLGTVSLVRVMD
                     GRLKPKEKIRLMAMQRDHLVEQIGIFTPKRKPVEELGPGEVGYVIAGIKDVRSARVGD
                     TITHVDRPADKPLPGFKEIKPRVFAGLYPVESSEYETFRDALAKLQLNDASLHYEPET
                     SQALGFGFRCGFLGMLHMEIIQERLEREYGINLITTAPTVVYEVVTANEVQLVENPAK
                     LPDPSRIVEIREPIIRATILVPQEYVGNVIGLCVEKRGIQKDMTYHGRQAMLVYELPL
                     NEVVLDFFDRLKSVSRGYASLDYDFVEFRAADLCKLDVLINGDKVDALSVIVHREQSQ
                     YRGRELANKLRELIPRQMFDVAIQAAIGSKIIARETVKALRKNVTAKCYGGDATRKRK
                     LLEKQKEGKKRMKQLGTVEIPQEAFLAVLRVDQK"
BASE COUNT         1809 a         2521 c         2776 g         1762 t
ORIGIN      
        1 cgggaagggg tgtgcgggtc ggacgggatg aatatcaacg gtcgcaacgg tctggcctgc
       61 atcacgccgc tgaaggacct gaaacaaccc atcgaactca aaccgctgcc gggggtgccg
      121 gtggtgcgtg acctgatcgt ggacctgagc cagttttaca gtgcctatcg gacagtgaag
      181 ccttgggtga tcaatcacga ccccgaaccg gagattgagc ggctgcaatc gcctgaggat
      241 cgggacaagc tggacggtct ctacgagtgc gtactttgtg cttgttgtac aacggcttgc
      301 ccgtcattct ggtggaatcc cgataagttc ctggggcctg cggcattgct gcaatcctgg
      361 cgtttcctgg cggacagccg cgaccaagcc accggcgagc gcctcgacgg cctagaagat
      421 ccttataagc tgttccgctg ccataccatc atgaactgca ccgaggtctg tcccaaggga
      481 ctgaacccgg ccaaggccat cgggcatata aaaatgatgc tggcgaaacg cgtgttatga
      541 tgggtgaatc gccttccccc tcatccccat cccttctccc tccaagggag aagggggcga
      601 gccgaagcgc acaagacgcg cgtcaattcg accgactgaa atggcgttgt cggcgcgggt
      661 tgctggaact ggatgtggtg ctcaaagact tcctcgacag gcgctattcc aacctgacac
      721 ccgccgaaca agaagcattt gagaaactat tgactacttc tgacgaaacc ctgttggcct
      781 atgtgcaggg caagcaaaac ccgcccgaaa aagagttaat gcagatcatt gcaaaactca
      841 gaaactaacc tgattttaaa cataaaagtt tcccgttttc tggccgggat ttttctggct
      901 gtgcatctgg gcggcatggc gctggtgact gccgttcctg tgatttggtc ggtgaggctc
      961 ggtctctggg gagtgctcgg atggagtctt tattattccc tcgtcaccca cgcctggcgc
     1021 tccagtgcct tggccatcac ggccattgaa atggataacg aagagcaggt ttcggtgcgc
     1081 cttgccggca gcgaggtatg gcaaacggca cagatcgcca gccgtttcgt gcatccctgg
     1141 ctgacgctga tgtcactgcg gctggagggc cggcgttggc cggtgagttt ggtgattgcc
     1201 gcggatgcgg tggaagcgga gccgttcagg cgttggcgcg tcgccctgaa acttcaggtg
     1261 gtgccggtgt gagcacggtg tcctgcgggg tacccgaaac gggctgtggg taatcgcggg
     1321 tgaagtgcaa accacgcgat tccttgcgcg cgagggcgga acgaatgatc agttcggata
     1381 ccaataatag attccgtaat tcaataagat cgttcgtaac tttaaagttg ctgtagaact
     1441 cccgaacctc ttccttcagc atctccaccc ggtgctgcgc ccgttgcagg cgcttggtgg
     1501 tgcgcacgat gccgacgtag ctccacatac cgcggcgcag ctcgtcccag ttgtgcgaca
     1561 ccacgacctg ttcatccggg tcagtgacac ggctctcatc ccaggccggc aaaccttccg
     1621 ggatcggtcc ggcggtgggc agctgtttca aaatatcctc ggccgcggcc ttggcgaaca
     1681 ccaggcattc cagcagggag ttgctggcga ggcgattggc cccgtgcagg ccggtaaaag
     1741 agcattcgcc cacggcataa agccggtcga gatcggtgcg ggcggaaagg tcggtcacga
     1801 ccccgccgca ggtgtaatgt gccgccggca ccaccgggat cggctcgcgc gtgatgtcat
     1861 agccgtattc cttgcagcgc tcgtagatcg tcgggaaatg gcttttgatg aaatccgccg
     1921 gcttgtggct gatgtcgagg tgcacgtaat cgagaccccc gcgtttcatt tcgtagtcga
     1981 tggcgcgcgc cacgatgtcg cgcgacgcca gctcgccgcg cgggtcgtgg tgttgcatga
     2041 acggcttgcc gttgggcaac gtaagtttgc cgccttcgcc gcgcaccgcc tcggaaatca
     2101 ggaacgactt ggcgcgcgga tggtacaggc aggtgggatg gaactggacg aactccatgt
     2161 tggccacgcg acaaccggcg cgccaggcca tggcgatacc atcaccagtc gaggtatcag
     2221 ggttgctggt gtagaggtag gccttgcagg ccccgccggt ggccagcacc acgaatcggg
     2281 cgctgtaggc gcggatatgg ccgtctgcct tgtcgagcgc gtacacaccg agactacggt
     2341 tgggtcccgg acggccgagc ttctggctgg tgatgaggtc gatggcgatg tgattttcat
     2401 gcaatgtgat gttcggatgc cggcgcacca ggccttccag cgtggtctcc atggcgcggc
     2461 cggtggcgtc ggcggcgtga atcacgcgcc ggtggctgtg tccgccttcg cgcgtcaggt
     2521 gatattccag gccgttgtgc ggatgttcct cgcggctgaa gggcacgcca cgctcaatga
     2581 gccagcgaat agatgttggg ccctcgcgca cgacaaattc caccgcatcc cggtggcaca
     2641 ggccggcgcc ggcgtcgagc gtgtcctgga tatgggattc gaagctgtct tcctcgccgt
     2701 tcagcaccgc ggcgacgccg ccctgggcgt agaggctgga accttcggta agggcggctt
     2761 tggccaaaat ggtgacccgt gccttatcgg cgatccgcag ggccaagctc aggccggcga
     2821 tgccgccgcc gatgatcagg acgtcggatg attgcaacgt ggtcatgcgg gatatgggcg
     2881 agaggagggc acgttggtga cgtttacgat atcctatcac acgagtcggc ctgtactgac
     2941 caggccgtcg cagcttcagg gacggaaaat tgaataataa ataccctctt tttcagactt
     3001 tcccggcgaa ctttggcctc tcctggcggt ctcttgatac cggtatgccc caccacgcgc
     3061 agggggtcca aaccccatga gcgagcggag cattgaccag aagctggttg tccgggtgca
     3121 gaagggcgac aaaaccgcct ttgacctgct ggtccggaaa tatcagcaca agatcgccaa
     3181 actgatctcg cgctacgtgc gcgatcggcg cgaagtggaa gacgtgacgc aggaggccct
     3241 catcaaggcc taccgtgcca tcaacggatt ccgtggcgag agcgcgttct atacttggct
     3301 gtaccggatt gccgtgaata cggcgaagaa ttatctggaa tcgcagggac gtcgcccgcc
     3361 cggaacggac atggaaattg aaacagcgga gcttgccgat ggcggttcca gtctgcgcga
     3421 gcaggccaca ccggagcgac agatgctgac ggacgaaatc gccaataccg tgcaccgggt
     3481 gatcgaaggg cttccggaag acttgcgcac ggcgatcacc ttgcgcgaga tcgagggcat
     3541 gagctacgag gagatcgcgc aggtcatgga ttgtccgatc ggcaccgtgc gttcgcgcat
     3601 cttccgcgcg cgcgaggcgg tcgatcagga attgcggcct ttgctggaga aatgagcatg
     3661 agcaacaccg atgagatgaa ggaaaagtta tccgcgctgg tggataacga actcgacgac
     3721 gtgaacgagc gtcgcgtcat ggcggtgctc gaaaaggacg ccgacctgcg ccgaacctgg
     3781 gagcgctatc atctggtgcg ctcggtcctg caccaggagc tggatgcatt tgtcccgcca
     3841 gacatggcgg agcacgtcgc ggcgcgaatc agtatggaac cggccaatac cagtgctttc
     3901 cgacgtcaaa aaattacccg ttacgccgga atgttcgcta tcgccgcctc cgtggcggcc
     3961 atcgctgtta cgagcgtgca gtggttaagc cgtcccatct cagcgcccgt gacgccagta
     4021 gcatcggttc agcccgcgcc ggacaatttc attcgtgccg gcgccacgcg ttgggacatg
     4081 aaacaaaatg aaccggaaac agaaagtgcg ttgaacgctt tcctggtcga gcataatgaa
     4141 tttgcctcgt cctccggcat tggcgggatg atgccttacg tgcgtgtcgt cggttacgat
     4201 aattctgcta aatgaatttt cgcaagctta ccccgtattt ctttttccct gcctgtctcg
     4261 ttttcagcac cgccgttctg gccggcgatg ccgcgcatga ctggctgatg aaaatcaacc
     4321 aggcggtgcg catgcttgat tatgaaggcg tttttgttta tcagcacgag aatcagctgg
     4381 aggccatgcg catcatccac aaggtggaga atggtgcggc ccgcgagcgg ctcgtgtcgc
     4441 tcaacggcgc gccgcgtgaa gtcattcggg atgagcgtga agttacttgc tattggccgg
     4501 acaaaaactc cgtgatggtg gaatatcgca aggcggacag caaggccttt ccagccatac
     4561 tgccggagcg catacaggat ctcgacgatt attatgcgat acggctgggc aacatggagc
     4621 gcatcaccgg ccggtctgcc cagctcgtga tcgtcaaacc ggtcgatcag tatcgctatg
     4681 gttaccactt gtgggcggac aaggagaccg gcctgttgct gaaggccgat ctgctcgata
     4741 tccagggcag gattctcgag cagttcatgt tcacgcaaat caatatcggc gcgaaaattc
     4801 ccgcctcggc gctcgcgccc ggcgtggccg gcaagggcat ggtctggcac cgggaagacg
     4861 aggatcgcaa aacgattacg gccaaggccg ggtggacggc gacgcaatta cccaaaggct
     4921 tccggctttc cgcccacatt ttgcgtcaga cgctgatgcg caagcgcccc atggagcatc
     4981 tggtgtacac ggatggattg gcggcggtgt ccgtgtttgt ggtgaaacgc gacgaagaca
     5041 gcaaaccttt catgctggga cccagccgca tgggggcggt gaacgcgctg ggttcgcgtg
     5101 ttgacgacta ccagatcacg accgtcggcg aggttccggc ggagaccatc gccctgattg
     5161 gcgggtcggt aacgccggtc gcgaatcagt aaatagacag gatatgcagg attaacaaac
     5221 gcgggtattt cctgctacta caatagaagt agctctataa gccccgtcta ttttcgtgac
     5281 agcaagatta cgttattcga tctttctcgt tctgtttttc tcggctccgc tggcggccgc
     5341 gcaacgcgcg ctgccggatt tcagccagct ggttaccacg cgcggcacgg cggtggtgaa
     5401 catcagtgta acgcgtgatc tcaagtctgt cggcttttca acggatacag tcgcgccgga
     5461 cgatgacagc gcgcgtgact ttacccgccg ttattccggc gagaacaccg cacctgccga
     5521 acagaattca ctcgggtcgg gattcatcat cagcccggat ggatacatca taacctgcgc
     5581 ccacgtgacc gagaacgcgc acgaaatcct ggtgcggctg aacgaccggc gggaatttag
     5641 cgcgcgtctg gtcggttccg accggcgcag tgacatcgcg ctgctgaaaa tcgaatccac
     5701 cgggctgccc acgattgtca tcggtgacgc gagcaggctt caggtgggcg aatgggtatt
     5761 ggccatcggt tcacccttcg gtttcgacaa ttccgccacc gccggcatcg tttccgccaa
     5821 ggggcggacg ttgccgaatg aaaattacgt gtcattcata cagacggacg tgcccatcaa
     5881 ccccggcaat tccggcggcc cgctgttcaa tctgaagggc gaggttgtcg gtgtgaactc
     5941 ccagatctac agcggcaccg gcagcttcat gggtgtgtca ttcgcgattc cgatcgacgc
     6001 cgccatgacc attggcgcac aacttaaaag cgaaggccgt gtgcgccgcg gctggctcgg
     6061 ggtgagtctg caggatgtct cgctcgcgtt ggcgaacgcc tacggcctga acaagccgcg
     6121 tggcgccctg gtggcggatg tgctccccaa gagcccggcg gcgaaatccg atctgcgtcc
     6181 cggcgacatt gtgatcgaat acgaaagcaa gcccattgac cgctcttccg atctgccgac
     6241 cctggtcggg ctctcgatgc cgggcacgca tgcccgcttt cgcgtcttcc ggcgcgatca
     6301 gggcatgcag accgttgtcg tgacattggg cgaactcaag gaagaatcgt cgccgcgacc
     6361 accactcgga aaagaccgca tggagggcca attacggttc ggtctgtcgc tgggagatat
     6421 tacggagcgg cagcggcggg aattcgatat cgatcatggc gcaagcgtga atgacgtgga
     6481 tgaaggacgc gcgcgcgatg ccggtctgcg cccgggcgat gtgattttgg aggtggatgg
     6541 taaacgcgtt acggatgttg ccggttttca ccggcttctg gcgcatgtgc gtcccggacg
     6601 acccgcggtg ctgcgcgtgc gacgcgggat ggcgacgctg tttctggccc tggataacat
     6661 caaccggccc gcgccctgaa atatttatgc gacccctgaa ataaggtatg ctacgcgaca
     6721 cagggcggac gtgaaccaca gaccacggcc ctgatcacat tgacacagac ccaaatcccg
     6781 ggcgcctttg gcgccttttt cgtatttccg acacgagcgg ctgatgccga tgcccgcccg
     6841 tgggggacag cgcgacgtct gtgaatgcca ggacgagcgc agcagagcta catttatgag
     6901 ctgggaccag gaacatatcc ggaatttctc gattatcgcg cacatcgatc acggcaagtc
     6961 cacgctggcg gaccgcttca ttcagacctg tggcgccctg gaggcgcgcg agatgcagga
     7021 gcaggtgctg gactccatgg acctcgagcg cgagcgcggc atcaccatca aggcccagag
     7081 tgtcgcgctc gattacaagg cgcgcgacgg ccagacctac cgcctgaaca tgattgacac
     7141 gccgggccac gtggattttt cctacgaggt atcgcgctcg ctgaccgcct gcgagggcgc
     7201 gctgctggtg gttgacgcct cccatggcgt gcaggcgcag acagtggcga actgctacac
     7261 agcagctgag ttggatctgg aaatcctgcc ggtactgaac aagatcgatc tgccttccgc
     7321 cgacccagcg cgcgcgatag cggaaatcga ggacatcatc ggaatcgacg ccggggatgc
     7381 catccgcgcc agtgccaaga ccggcgaggg cgtgacggat atcctcgagg ccatcgtgcg
     7441 gcgcattccc gtgccgaaag gtcgggccga ggccccgctc aaggcgctga tcatcgattc
     7501 ctggttcgac aactacttgg gcacggtatc gctggtgcgt gtcatggatg gacgcttgaa
     7561 gcccaaggaa aaaatccgcc tcatggccat gcaacgagat catctcgtcg agcagattgg
     7621 tatattcacg ccgaagcgca aaccggtgga agagctgggt ccgggcgagg tggggtatgt
     7681 cattgccggc atcaaggatg tgcgcagcgc gcgcgtcggc gacactatta cgcatgtgga
     7741 ccggccggcg gacaagccgt tgcccggttt caaggagatc aagccgcgcg tgttcgccgg
     7801 actgtatccg gtggaatcct ctgaatacga gacttttcgc gatgcattgg cgaaactgca
     7861 actgaacgac gcctcgttgc actatgaacc ggaaacttcg caggcactcg gttttggttt
     7921 tcgctgcggg tttctcggca tgttgcacat ggagattatt caagagcggc ttgaacgcga
     7981 atatgggatc aatctcatca ccacagcccc aacggtcgtc tacgaggtcg tgacggcgaa
     8041 cgaggtgcag ctcgtggaga atccggcgaa actgccagac ccgtcccgga tcgtcgaaat
     8101 ccgcgaaccc atcatccgcg ctaccatcct ggtgccgcag gaatatgtcg gtaatgtcat
     8161 cgggttgtgc gtggaaaaac gcggcattca gaaagacatg acctatcatg gccgccaggc
     8221 gatgctggtg tacgaacttc ccctgaacga ggtggtgctg gacttcttcg accggctcaa
     8281 gtccgtcagt cgcggatacg cctcgctcga ctatgatttc gtggagtttc gcgccgccga
     8341 cctgtgcaag cttgacgtgc tgatcaatgg cgataaagtg gacgccctgt cggtgatcgt
     8401 gcaccgcgag caaagccagt atcgcggacg tgaactggcg aacaagctgc gcgagttgat
     8461 cccgcggcag atgttcgacg tcgccatcca ggcggcgatc ggttcaaaga tcatcgcgcg
     8521 cgaaacggtc aaggcgctgc gcaagaacgt gacggccaag tgctacggcg gcgatgcgac
     8581 gcgcaagcgc aaattgctgg aaaaacagaa ggaaggaaag aaacgaatga aacaactggg
     8641 taccgtggaa attccgcaag aggcctttct cgccgttttg cgcgtggacc agaaataacg
     8701 ccatggactt cgcgctgatc atgttgctga ttctggttgg caccggcacc ctctggggtc
     8761 tcgaccgctg gctgtgggcg gccggtcgcg agcgtcgcgc cagggcgctg gcggacgcag
     8821 gcaaggctga ggaggctgcg ctggcgcgca aagaaccgat ctacgtgg
//
LOCUS       DBNGZE010000424         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_753737, whole genome shotgun sequence.
ACCESSION   DBNGZE010000424 DBNGZE010000000
VERSION     DBNGZE010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_753737"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="ccoG"
                     /locus_tag="AC16FV_10940"
     CDS             <1..890
                     /gene="ccoG"
                     /locus_tag="AC16FV_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR2850604.1"
                     /translation="GPWETFWILFYGFATYGNAGWLREQVCLYMCPYARFQGAMFDRN
                     TLIISYDKARGEPRGSRTRGIEPRLQGLGDCIDCTLCVQVCPTGIDIRHGLQIGCIAC
                     AACVDVCDSVMDKMGYPKGLIRYTSENALEGKPVKLLRPRVLIYTGILTAIAAALLIA
                     VLLRVPLKLDVLRDRNALFRETFDDLIENTYTLKVMNMDNGPHRYVLSAHGIEGLQLR
                     TDRPEITVAAGEVAEVPVSLQADRSNLSQRSSPVYFRLQAVDTDGLATEQTARFLGPE
                     PEEHHKEHDTEHGKKDEKH"
     gene            865..1374
                     /locus_tag="AC16FV_10945"
     CDS             865..1374
                     /locus_tag="AC16FV_10945"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR2850605.1"
                     /translation="MEKKTKSINPWYREPFVWLLIAFPLTAVIAGFITLGLAISSDDG
                     LVEDDYYRRGKEINRVLARDQAAATHGLEGRVELDDAQHQLLIRLSARESATIADNVE
                     LKFLHATRAGVDKTLILARQPDGSYHAPLPELAQGHWNVQLAAQDWRLVGSLFVPGAR
                     GFDIRPSLP"
     gene            1529..>2105
                     /locus_tag="AC16FV_10950"
     CDS             1529..>2105
                     /locus_tag="AC16FV_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2850606.1"
                     /translation="MPTEITLVSAFLVGLLGSAHCLGMCGGIVGALTLGLHEDIRRSP
                     TRLFPYLLAYNTGRIASYAVPCALVGFMSAQILHIAPPAQARLAAKIITGGFMIALGL
                     YLAGWWLGLTALERLGGKLWVRIEPLGRRFLPVNHPAKALALGLVWGWLPCGMVYAAL
                     AWSLATGDAARGALLMLAFGLGTLPMLFAMGA"
BASE COUNT          357 a          687 c          654 g          407 t
ORIGIN      
        1 tcgggccgtg ggagaccttc tggatcctgt tctacggttt cgccacctac ggcaacgccg
       61 gctggctgcg cgagcaggtg tgcctgtaca tgtgcccgta cgcgcgcttc cagggcgcga
      121 tgttcgaccg caacacgctc atcatctcct acgacaaggc gcgcggcgag ccgcgtggct
      181 cgcgcacgcg cggcatcgag cccaggctcc aggggctggg cgactgcatc gactgcaccc
      241 tgtgcgtgca ggtttgtccc accggcatcg acatccgcca cggcctgcag atcggttgca
      301 tcgcctgcgc cgcgtgcgtg gacgtgtgcg attcggtgat ggacaagatg ggctatccca
      361 aggggctgat ccgctacacc agcgagaacg cgctcgaagg caagccggta aagctgttgc
      421 gcccgcgcgt cctgatctat accggcatcc tgacggcgat tgccgcggca ttgctcatag
      481 cggtattgtt gcgcgtgccg ctcaagctcg acgtgctgcg cgaccgcaac gcgctgtttc
      541 gcgagacttt cgatgatttg atcgagaata cctatacact caaggtcatg aacatggata
      601 acggaccgca ccgctacgtc ctcagcgcgc acggcatcga agggctgcaa ctgcgcacgg
      661 accgtcccga gatcacggtg gcggcgggcg aggtggcgga agtcccggtg agcttgcagg
      721 ccgatcgcag caatctctcg caacgcagct cgccggtcta tttccgtttg caggcagtcg
      781 ataccgacgg actcgcaacc gagcagactg cacgcttcct cgggccggag cccgaggagc
      841 atcataagga acatgacacg gagcatggaa aaaaagacga aaagcattaa cccttggtat
      901 cgcgaaccct tcgtatggtt gttgatcgcg ttcccgctca cggccgtgat cgcgggcttc
      961 atcacgctgg ggctggcgat atcgtcggac gacgggctgg tagaagacga ctattaccgc
     1021 cgcggcaagg agatcaaccg cgtgctggcg cgcgatcagg ctgccgccac gcatggcctg
     1081 gaaggtcgcg tggaactcga tgacgcgcag catcaattgc tgatccggct cagcgcgcgc
     1141 gaatcggcca cgatcgcgga caacgtcgag ctgaaatttc tgcacgccac gcgcgccggc
     1201 gtcgacaaga cgctgatcct cgcgcgccag cccgacggca gttatcacgc accgctgccg
     1261 gaattggcgc agggccactg gaacgtgcag ctcgcggcgc aggactggcg tctcgtcggt
     1321 tcgctgttcg tgcctggcgc acgcggtttc gatatccgcc cgtcgctgcc ctagcaattt
     1381 aaattccgtc atgcccgcgg aggcgggcat ccaggaaaat cataataagt tttctggttc
     1441 cccgccttcg cggggacgac gtccggattc ccgctttcgc gggaatgaca acaaggatta
     1501 gtcactttta attttttaat tcttgaccat gcccacggaa atcaccctcg tctcggcctt
     1561 cctcgtgggc ctgctgggct cggcgcattg cctcggcatg tgcggcggca tcgtcggcgc
     1621 gctcacgctc ggcctgcacg aagacatccg ccgttctccc acgcgcctgt ttccctatct
     1681 cctcgcctac aacaccggcc gcatcgccag ctacgccgtc ccctgcgcgc tggtcggatt
     1741 catgagcgca cagatattac acatcgcccc gccggcacag gcacgcctcg ccgcgaaaat
     1801 catcactggc ggtttcatga tcgcgctcgg cctgtatctc gctggctggt ggctgggcct
     1861 gacggcgctg gaacgtctcg gcggaaaact ctgggtgcgc atcgagcctc tcggccggcg
     1921 cttcctgccg gtgaatcacc cggccaaggc gctcgcgctc gggttggtct ggggctggct
     1981 gccctgcggc atggtctatg ccgcgctggc ctggtcgctg gccacgggcg atgctgcccg
     2041 cggcgcgctt ctgatgctgg ccttcggttt gggcacgttg cccatgctgt tcgccatggg
     2101 cgccg
//
LOCUS       DBNGZE010000425         4703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_197915, whole genome shotgun sequence.
ACCESSION   DBNGZE010000425 DBNGZE010000000
VERSION     DBNGZE010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_197915"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16FV_10955"
     CDS             <1..355
                     /locus_tag="AC16FV_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2850607.1"
                     /translation="VSNGILLVDYTNRLRGRGMRLEDAVVLGGRTRLRPILMTTLCTI
                     LGLIPMAIGLGEGAESNAPMAIAVIGGVLVSTLLTLFVVPCVYELLSRFERDRRAVEA
                     RRREIAQVTKDVAGG"
     gene            583..1431
                     /locus_tag="AC16FV_10960"
     CDS             583..1431
                     /locus_tag="AC16FV_10960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850608.1"
                     /translation="MNKVEAKQKQHKSWTMVAPGWGKYDPLLRAWAEPVTERMIALAG
                     IREGSRVLDIACGAGEPALTVAEKVGPQGAVLATDFVEEMVGYARSKAQTRALSNIEF
                     RCIDGEVLDVPNGSFDAVTMRWGLMFMPDPVACMHCANKALRPGGKAVVSCWTEAANN
                     PFVTVPLGVLKRHMEIPDPPAGAPGIFALANPDRLRAIFTEAGFRDVTIEQVTVPMAD
                     FATGAEYDEFIRELAGPVASLFVQLPADKQAVVKQEIARDAEARSSRPGRVYLHGVTH
                     VVVGTK"
     gene            1481..2197
                     /locus_tag="AC16FV_10965"
     CDS             1481..2197
                     /locus_tag="AC16FV_10965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2850609.1"
                     /translation="MSSLKDKVVIITGAVGNLGHAVAKHVQAQGGRTILVDRSSDRLR
                     KIYSELKSSTQHWLAGDVDMTDPKAVNAMAAEAHQRFGRLDGLVNTVGGFRGGKPVHE
                     TDLSEWDFLYDINVRTTLNACRAVIPYMLQVQSGRIINIASRNAFQGSPNYAAYSAAK
                     TAVLRLTESLAGEMKTRGINVNCIVPGTIDTPQNREAMPQADFSAWVPPEDLAKVIAF
                     LLSDDARSVTGVAVPVYGRS"
     gene            complement(2202..3509)
                     /locus_tag="AC16FV_10970"
     CDS             complement(2202..3509)
                     /locus_tag="AC16FV_10970"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2850610.1"
                     /translation="MDLHRIVIVGGGAGGLELATRLGDKFGKPKRARVTLVDAKLTHL
                     WKPLLHEVAAGTFDSHENDVDYIAQAHQHHFDYRLGRMDSLDRDRRELSLAPTLDEDG
                     REIIPRRAIPYDTLVLALGSMSNDFGIPGVREHCVFIDDRAQADAFQRAVMRNYIHAQ
                     THPERPAAERLQLVIVGAGATGVELAAEMHNMARQLVVYGFDRIVPERDLKIVIIEAT
                     DRILPALPKRLSEAAQNELHKLGVEVHTRRRVTRVIAEGVQTQDGQFFHATLKVWAAG
                     IKAPEFLHNLAGLETNRINQLVVRPTLQTTYDDDIFAIGDCAACPQPGKDTLVPPTAQ
                     AAHQQAALLVKSIARRLDGKSLPTFTYRDRGSLISLSHYTTVGNLMGNLTGDVMIEGR
                     FARLTYRMLYRLHQRALHGTLPMLLLMFSDFLQHRAHPRMKLH"
     gene            3947..4588
                     /locus_tag="AC16FV_10975"
     CDS             3947..4588
                     /locus_tag="AC16FV_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HIR2850611.1"
                     /translation="MDRVSKKKRWIYFLAIFIFATAPAKAATLSFFCITGNLAADCVT
                     GEAQLTVNVTDPGGNQVLFHFKNSGPNASSITDVYFDDGSLLGLSQITNGPGVSFSQY
                     ASPPNLPGANNVSPPFQVTAGFLADSNPPVQPNGVNPGEWLKIYFDLQAGKNFADVIT
                     DLTDGSLRIGLHVQGFTGGGSESFVNNPVPIPAAVWLFGAGLGLLSAFARRKK"
BASE COUNT          908 a         1327 c         1516 g          952 t
ORIGIN      
        1 ggtcagcaac ggcatccttt tggtggatta caccaaccgc ctgcgcgggc gagggatgag
       61 attggaggat gctgtggtcc ttggggggag gacaagactg cggcccattc tcatgaccac
      121 gctctgcacg atcctgggac tcattcccat ggccatcggg ctgggcgaag gagcggaatc
      181 gaatgccccc atggcgatcg cggtgatcgg cggtgtgctg gtgtcgacgc tgcttacgct
      241 gttcgtcgtg ccctgcgtgt atgaactgct gtcacgtttc gagcgcgacc ggcgcgcggt
      301 ggaagcgcgg cgacgggaga tcgcccaggt gaccaaggac gtggccggag gttgatgtgg
      361 ccagtgtcgg aataaagccg gcgcggggct gtgcgaggcg aaagtcttgc tcgtcaacca
      421 aggggaaaga acctttcgtg atacgcgccg gccggcgcat cgcgcggatg gtgatcgcca
      481 aagtcgaaca cgtcgcattt tgcataaacc gaagaattgt atgacactgc acgctgtgct
      541 ggtggccacg gacatatcgg actaaaaaag aggagacggg acatgaacaa ggtggaagcg
      601 aagcagaagc agcacaagag ctggaccatg gtcgctccgg gttggggcaa gtacgacccg
      661 ctgttgcgcg cctgggcgga gccggtgacc gagcgcatga ttgcgctcgc gggcattcgc
      721 gagggttcgc gggtgctcga catcgcttgc ggcgcgggcg agccggcgct gaccgtggcc
      781 gagaaggtcg gcccccaagg cgcggtgctt gccaccgact tcgtcgagga aatggtcggc
      841 tacgcgcgtt ccaaggcgca gacgcgcgcg ctctccaata tcgagttccg ctgtatcgac
      901 ggcgaagtcc tggacgtacc gaatggcagc ttcgacgccg tgaccatgcg ctggggcctc
      961 atgttcatgc ccgatccggt cgcttgcatg cattgcgcca acaaggcgct ccggccgggc
     1021 gggaaagcgg tggtcagctg ctggaccgaa gcggcgaata atccgttcgt cacggtgcca
     1081 ctgggggtgc tcaagcggca tatggagatt cccgatcccc ccgcgggcgc gcccggtatc
     1141 tttgcgctcg ccaaccccga tcggctgcgc gccatcttta ccgaagcagg ttttcgcgac
     1201 gtaaccattg aacaggtgac ggtcccgatg gccgattttg cgaccggcgc cgagtatgac
     1261 gagtttattc gcgagctcgc gggacccgtg gcgagcctgt ttgtacagtt gccggcggac
     1321 aagcaagcgg tcgtgaagca ggagattgcg cgcgacgccg aggcgcgcag ctcaaggccc
     1381 gggcgcgtgt atctccacgg cgttacccac gtcgtcgtcg gcaccaaata atagaccgag
     1441 atgtctgtga aagtatggga atcccggcga gcgaacgagt atgtccagtt tgaaagacaa
     1501 ggtggtgatc atcacgggcg cggtcggcaa tctcggccac gccgtggcca agcacgttca
     1561 ggcgcagggc ggcaggacga ttctggtcga ccgttccagc gatcgcctgc ggaaaatcta
     1621 cagtgagttg aaaagttcca cccagcactg gctggcgggc gatgtcgata tgaccgaccc
     1681 gaaagcggtg aacgccatgg cggccgaggc gcaccaacgc ttcgggcggc tggacgggct
     1741 ggtgaacaca gtcggtggat ttcgcggcgg caagccggtg cacgagaccg atctgtcgga
     1801 atgggatttt ctgtacgaca tcaatgtgcg caccacgctc aacgcctgcc gcgccgtgat
     1861 tccgtacatg ttgcaggtgc agagcgggcg catcattaat atcgcgagcc gcaacgcgtt
     1921 tcagggcagc cccaactacg ccgcctacag cgccgccaaa accgcggtgc tgcgcctgac
     1981 cgagagcctg gcgggtgaga tgaagacgcg cggcatcaat gtcaattgca tcgtacccgg
     2041 gaccatcgat acgccgcaga accgcgaggc catgccccag gcggatttct ccgcctgggt
     2101 cccgcccgag gaccttgcca aggttattgc attcctgctg tcggatgacg cgcgttcggt
     2161 taccggcgtc gcggtgccgg tgtacggccg cagctgacag atcaatgcag cttcatccgc
     2221 ggatgcgcgc ggtgctgcag gaaatccgag aacatgagca gcagcatcgg cagtgtgccg
     2281 tgcagcgcgc gctggtgcag gcggtagagc atgcggtaag tcaggcgcgc gaaccggccc
     2341 tcgatcatca cgtcgcccgt gagattgccc atgagattgc cgaccgtggt gtagtgactg
     2401 agcgaaatga gcgagccacg gtcgcggtaa gtgaaggtcg gtaatgattt gccgtcaagg
     2461 cggcgggcga tggatttaac cagcaacgcc gcctgctggt gcgcggcctg tgccgtcggt
     2521 ggcaccagtg tgtccttgcc cggctgcggg caggcggcgc aatcgccgat ggcgaaaata
     2581 tcgtcgtcat aagtggtctg caatgtcggt cgcaccacca gctgattgat ccggttggtt
     2641 tcgaggccgg cgagattgtg cagaaactcc ggcgccttga tgccggcggc ccagaccttg
     2701 agcgtggcgt gaaagaactg gccgtcctgc gtctgcacgc cttcggcgat tacgcgcgtc
     2761 acgcgccggc gcgtgtgtac ttcgacgccg agcttgtgca attcgttttg cgccgcttcg
     2821 gacagccgtt tcggcagcgc cggcaggatc cggtccgtgg cttcgataat cacgatcttc
     2881 agatcgcgtt ccggcacgat gcggtcgaaa ccgtagacca ccagctggcg cgccatgttg
     2941 tgcatctccg ccgccagttc cacgcctgtg gcgccggcgc cgacgatcac cagttgcagc
     3001 cgctcggccg ccggccgctc cggatgcgtc tgcgcatgga tgtaattgcg catcacggcg
     3061 cgctggaagg cgtcggcctg tgcgcggtcg tcgatgaaca cgcaatgctc gcgcacgccg
     3121 ggaatgccga aatcattgct catgctgccg agcgcgagca cgagggtgtc gtacgggatg
     3181 gcgcggcgcg ggatgatctc gcgaccgtcc tcgtcaaggg tcggtgccaa gctcagctcg
     3241 cgccggtcgc ggtcgagact gtccatgcgc ccaaggcggt agtcaaagtg gtgttggtgc
     3301 gcttgggcga tgtaatcgac gtcgttctcg tgcgaatcga aagtgccggc ggcgacctcg
     3361 tgcagcagcg gcttccacag gtgcgtgagc ttggcgtcca cgagcgtgac ccgcgcgcgt
     3421 ttcggtttgc cgaacttgtc gccgaggcgc gtggccagct ccaatccgcc cgcgcctccg
     3481 ccgacgatca caatccggtg gagatccatg tgggcattct agcgtggcgt tacccggcgc
     3541 ctgtcccagg ctgtttcatg catgtttcat ttttcacgga agtgattttt gcagagctgg
     3601 tgcggtaaat ccggtgatgg gcatttgtgt tgaatttatt tacataagga atcttgggtt
     3661 attacttggg ttcatcgtga aatttggaaa atgcttagcc agctttggaa aatagccacg
     3721 attcaatgtc ggcgaaattt acatttcatt ctttttttca tatcgtaact tggcaacttt
     3781 tatctattcg tatgatttca aaagaaaaat agatttctgg aatgctttgt gctctataga
     3841 tagcgcgagt gcgagcagac tcgagggcag tgctgcaaga cttggttgtc tttttaggag
     3901 cctgcggctc ggagtatacc cggccgtgga ttgagggaga tgaaacatgg accgtgtttc
     3961 aaaaaagaaa cggtggattt attttctggc gatattcatc ttcgccacgg cgccggcgaa
     4021 ggcagccacg ctgtccttct tctgtattac cggcaacctt gccgccgact gcgtgaccgg
     4081 agaggcccag ctcacggtca atgtcaccga tcccggtggg aatcaggtgc tgttccattt
     4141 caaaaactcc gggccgaatg catcctcgat taccgatgtc tatttcgacg acggctccct
     4201 attgggcttg agccaaatca cgaatggtcc gggcgtcagc ttcagtcagt acgcctcacc
     4261 acccaatctg ccaggcgcca acaacgtaag cccgccgttt caggtcaccg caggctttct
     4321 ggccgattcc aatcctcccg tgcagcctaa cggtgtgaac cccggcgagt ggcttaaaat
     4381 ctatttcgat ctgcaagcgg gcaagaactt cgccgatgtg atcaccgatt tgaccgatgg
     4441 atccctccgc atcggtctgc atgtgcaggg atttaccggc ggcggcagcg agagttttgt
     4501 gaacaatcca gtgccaatcc cggcggcagt gtggctgttc ggtgcaggat tgggtctgct
     4561 gtccgcattc gccagaagga aaaaataacc cggcgtttgc tgcaaggggg atattcgcca
     4621 catcgtggcc agtggctgtt taattcgcag ctaaaggcag ggggcgatta caacaagctt
     4681 cttgtccaga gaaggaagag gag
//
LOCUS       DBNGZE010000426         3962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1379232, whole genome shotgun sequence.
ACCESSION   DBNGZE010000426 DBNGZE010000000
VERSION     DBNGZE010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1379232"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            884..1564
                     /locus_tag="AC16FV_10980"
                     /pseudo
     CDS             884..1564
                     /locus_tag="AC16FV_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587404.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            1655..>3962
                     /locus_tag="AC16FV_10985"
     CDS             1655..>3962
                     /locus_tag="AC16FV_10985"
                     /inference="COORDINATES: protein motif:HMM:NF053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimL-related protein"
                     /protein_id="HIR2850612.1"
                     /translation="MSTHSKVDLSTLGWVKTEIDETLKQARLALESFAENPSDKTRLR
                     FCITHLHQVVGTLLMVELDGAAMLAKETEALADDVINDKVVPDSRTLDALTRGILTLP
                     DYLARLQAGQSDVPLKHIGLMNEMRAVRNATPISELELFSPDLSVRPPPSESHAEKLA
                     EGEYRDLAKQLRSGFQPALLDWLRDASNKVPLDKIADIFSQLQSKTGVSVLEQLFWVA
                     GGFVEAVRDDGLEVTAERKKLLGRLDQHIKKIIDGADKSTVRIASEDLTRALLWEVAQ
                     AKSGGARITQLKRAFDLEFLLSGDTAEAAAASDLPTPEALASVSTALGKEIEAAQDLM
                     STYFDPESRGTTSLEPLLESFHKMSGTLDMLNVPILKSLVDELSLTCRAVIDNKITNP
                     DVISMPMAEALLMVESSTRDISRSPAEWKKQIEDAIRHLHGLHSPEEADVMPATDGFE
                     VSDGELTESDYKQLLSVVAGEVGVNLGRIEEALEGFAADPARVESLDDVPQLLSQILG
                     AMQILGQARAAELVETTKGHVENIRRGTLVADSAIMDGLAVSVGTIGAYVEGLRAERP
                     NLESLIDAALKEMETALASGHGKNRDPALLIDGVRLSLESWLDDRENEAALGTLTQQL
                     EELTQLAQEQGQEKIARISAEMNHLLALVAQDPSRVSEEIVETLKQSFTAMSALAGRR
                     MTLRTAAPAAREPEPEPESEMAPPPPVESKKKTPAAPAAPPPAPTRSAPDAEFDAEIM
                     EIFIEDAREVLENIKNKFLVWRDDTDNQN"
BASE COUNT          830 a         1238 c         1279 g          615 t
ORIGIN      
        1 ggccatcaac cagatgagcc cgatgctgct gacggttacc gacgagatcg tggaactcgg
       61 cgccaaggct gggctgaaac aggaccagct ttacctcgcc agccgccagg gcatgttgtc
      121 gcaacgcatc gccaaggacg tcaacctgtt ctcgcagggc ggtaacgaag ccgcggtggc
      181 ggcggcgcag ttcggcaagg acgccaagct cttcaaggag tccgacgaca aattgcgcaa
      241 ggccgcgccg cccgccgttc gcgagaagct cgatgaatcc gccgccattt tcggcgagat
      301 caacaaccac gtcgaaggca ttctgggcaa cgcggcggag ctgttcgtct cccagcgcgc
      361 cagccagacc gtgttcgagc agtccgatcc gctgctgaaa aagtcgacgg aactggtgga
      421 cgcctactcg aaactggccg agcaacgcgc cggtatccgc acctccatct tcgtcgccgg
      481 ttttgccacg gtgctgttcc tgttcctgct ggcccgcaaa ctgatcgcgg acgcccgtga
      541 tcgtgcgaaa caaagttcgg aacagaaccg tcagagccag gacgcgattc tcaagctgct
      601 cgatgaaatg ggcgacctcg ccgacggtga cctcacgatt cagcccgagg tgaccgagca
      661 gatcacgggc gcgatcgcgg actcgatcaa ctacgcggtg cgcgaaatgc gcaacctcgt
      721 cgtgcgcatc aagaacgccg cgcaacaggt ggccgtcgct tccgagcact cacgccagac
      781 agcgaccgag ctgaccgaag ccgcgttgcg gcaggcggcg cagatcaccg aggccaccgg
      841 caagatggtg gcgatggcgc gctcgatgga agacatgtcc aagagcgccg agcgttccgc
      901 cgaagtggcg cagggctcgg tggcgaccgc caagcgcggc gccgcggcgg tgcaggacac
      961 gatcacgggc atggacggca tgcgcgagca gattcaggaa accgcgaagc gtattaaacg
     1021 tctcggtgaa tcgtcacagc aaatcggtga aatcgtggaa ctcatcaacg acatcgccga
     1081 gcagaccaac atcctttcat tgaacgccgc gattcaggcg gctatggcgg gagaggcggg
     1141 acgcggcttc gcggtggtgg cggacgaagt gcagcgactg gcggagcgct cggccgaggc
     1201 gaccaagcag atcgccgacc tggtgaaaac cattcaggcc gacaccaacg aagcggtggc
     1261 ctcgatggaa caggcgacca acggcgtggt gcaggcaacg cgcctggccg acgctgcggg
     1321 acaggcactg ggcgagatcg aatcggtgtc ctagcagctg tcgagcctga ttgtgaacat
     1381 cgcgcgcgat gctcaacgtc agtcccagac ggcgaccgac gtttcaggga gcatggcgaa
     1441 aattcaggaa accaccacac tgacctcctc gggcagccgg caaacggcgg aagccatcgg
     1501 caagctctcc gatctggcgc gcgagctgca ggcctcggtg gcgggtttca agctgccggc
     1561 atagcgtcgc ccgccgggcg atgatgtacg gccagggaaa tcacgcctgt tcccgggagg
     1621 gacgggcgtg acgcggcgga gtttggcgat accgatgagc acgcacagca aagttgacct
     1681 gagcacgctg ggttgggtca agaccgagat cgacgagacg ctgaagcagg cgcgtctcgc
     1741 gctcgaatcg ttcgcggaaa atccctccga caagacgcgc ctgcgtttct gtatcaccca
     1801 cctgcatcag gtggtcggca cgctgctcat ggtcgagctc gacggcgccg ccatgctcgc
     1861 caaggaaacg gaagcgctgg ccgatgatgt catcaacgac aaggtcgtgc ccgattcgcg
     1921 aaccctcgac gccctcacac gcggcatcct gacgctgccc gactatctcg cgcggctgca
     1981 ggcgggccag tcggatgttc cactcaagca tatcggcctc atgaacgaga tgcgcgcggt
     2041 gcgcaacgcc acgcccattt cggaactcga acttttttcg cccgacctgt cggtgcgtcc
     2101 gccaccctcg gaaagccacg cggaaaaact ggcggaaggc gaataccgtg atctggccaa
     2161 acagcttcgc agcgggttcc agccggcact gctcgactgg ttgcgcgacg catcgaacaa
     2221 ggtgccgctg gataagatcg ccgatatttt ctcccagctg cagtcaaaga ccggtgtctc
     2281 cgttctggaa caattgttct gggttgccgg cggttttgtc gaggccgtga gggatgacgg
     2341 cctcgaggtg acggccgagc gcaagaaatt gcttggccgg ctcgaccagc acatcaagaa
     2401 aatcattgac ggcgcggaca agtcgacggt ccgcattgcc tccgaggatc tcacgcgcgc
     2461 gctgctgtgg gaggtggcgc aggcgaaatc cggaggcgcc aggatcacgc agctgaaacg
     2521 cgcgttcgat ctggaattcc tgttgtccgg cgatacggcc gaggccgcgg cggcgtccga
     2581 cctgccgacg cccgaagcgc tggcctcggt gtccaccgcg ctcggcaagg agatcgaggc
     2641 ggcgcaggat ctgatgtcca cctacttcga tccggaaagt cgcggcacga cctcgctcga
     2701 accgctgctg gaatcgttcc acaagatgtc gggcacgctc gacatgctga acgtgccgat
     2761 actgaagtct ctggtggacg agctgtcgct cacctgtcgc gccgtgatcg acaacaagat
     2821 cacgaacccc gatgtgatct ccatgccgat ggcggaagcc ctgctcatgg tggagagcag
     2881 cacccgcgac atcagccgtt cgcccgccga atggaaaaag cagatcgagg acgccatccg
     2941 ccatcttcac ggcctgcatt ctccggaaga ggccgatgtc atgccggcga cggatggctt
     3001 cgaagtatcg gacggcgagc tcaccgagtc cgattacaag caattgctga gcgtggtggc
     3061 tggcgaggtc ggcgtcaatt tgggccgcat cgaggaagct ctcgaaggtt tcgccgccga
     3121 ccccgcgcgc gtggaatcgc tcgatgacgt gccgcagctc ctgtcccaga ttctcggggc
     3181 catgcagata ctcgggcagg cgcgcgccgc cgaactggtg gagaccacga agggccacgt
     3241 ggaaaatatc cgtcgcggaa cgctcgtcgc ggacagcgcg atcatggatg gactcgcggt
     3301 gtccgtgggc accatcggcg cctacgtgga aggcctgcgc gccgaacgcc cgaatctgga
     3361 gagcctgatc gatgcggcgc tcaaggaaat ggaaacagcc ctggccagcg gccacggcaa
     3421 gaaccgcgac ccggcgctgt tgatcgatgg ggtgcgcctg agcctcgaat cctggctcga
     3481 cgatcgcgaa aacgaggccg cgctggggac gctcacgcag cagctggagg agctcaccca
     3541 gctggcgcag gagcaagggc aggaaaagat tgcgcgcatc agcgcggaaa tgaatcacct
     3601 gctggcactg gtggcgcagg acccgtcgcg ggtgtcggaa gagattgttg aaaccctgaa
     3661 acaatcgttc accgccatgt cggcactggc cggacggcgc atgaccctgc gcaccgccgc
     3721 tcccgcggcg cgcgaaccag agcccgagcc tgaatcggaa atggcgccac caccgccggt
     3781 cgaatcgaaa aagaaaactc cggcggcgcc ggccgccccg ccgccggctc cgacccggtc
     3841 cgcgccagac gcggaattcg atgccgagat catggagatc ttcatcgagg acgcgcgcga
     3901 ggtgcttgag aacatcaaga ataaattcct cgtctggcgc gacgacaccg ataatcagaa
     3961 cg
//
LOCUS       DBNGZE010000427         3951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_269061, whole genome shotgun sequence.
ACCESSION   DBNGZE010000427 DBNGZE010000000
VERSION     DBNGZE010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_269061"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC16FV_10990"
     CDS             <1..659
                     /locus_tag="AC16FV_10990"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR2850613.1"
                     /translation="VLNALGENWTIRLDGYEATNFGLLSGNAQWQKDMSMKIINAAIK
                     CGAKLLILPECGHAYGALRWQGANMYGKPLPFKVMHISEYLAENLNNGKLKVKPVAKS
                     VTFHDPCQISRRGGATRAGRDVLKALGVELREMTPTGDMNWCCGGGGGVVTIHRADEL
                     RYKVFEIKMKQVNDTGAEMLVMSCSNCRQTFDDGQEHFKWDKTANSLLEMVADNLVEG
                     "
     gene            663..2435
                     /locus_tag="AC16FV_10995"
     CDS             663..2435
                     /locus_tag="AC16FV_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR2850614.1"
                     /translation="MATQTVVVDPITRIEGHLRIEAKTDGNGVITQASSAGTMVRGIE
                     IILRGRDPRDAWAFAQRICGVCTLVHGIASVRAVEDALKYEIPANAQLIRNLMIGAQY
                     VHDHVMHFYHLHALDWVDVVSALKADPKKTSELAQAISNWPKSSPGYFADMQKKVKNF
                     VEAGQLGIFANGYWGHPAYKLPPEANLMAVAHYLEALAWQRDVVQIHTIFGGKNPHPN
                     FLVGGTPAAISLHPQQQGQGKGPHYRGGAGATAVNMVGLQRVKNVIDSMRAFVDQVYV
                     PDTLAIAGFYKDWGAQGEGVGNFLCYGDFPSKGTNDVNSFLVPRGVILNRDLSKIHEV
                     DLNAEDQIQEFVAHSWYDYSGGKDKGLHPYKGETKFNYTGPKPPYEYLDTDKSYSWLK
                     SPRWKGKAVEVGPLARVLMLYAKGHKQTKELVHMTLKQLDVPVGALFSTLGRTAARTL
                     ETKIFVDALEGWYNQLIANIKAGDVKTFNEKLWEPSTWPKEAKGVGYTEAPRGALAHW
                     IVIKDGKIDNYQAVVPSTWNAGPRDPSGQEGPYEAALKGHKLHDAKQPVEILRTIHSF
                     DPCIACAVHVTDPEGEELIKVKVS"
     gene            2447..3031
                     /locus_tag="AC16FV_11000"
     CDS             2447..3031
                     /locus_tag="AC16FV_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR2850615.1"
                     /translation="MKRGYQITAALSALFLAGTAQAHTFGAHGAGFAAGFAHPFIGLD
                     HLLAMLAVGVWAAQLGGRAIWRVPLAFMAMMLLGSALALGSLPLPAVETGVASSILVL
                     GLLIAVAARFPLVVSMLLVGAFALFHGHAHGQELPQAASASLYSLGFLLATGLLHATG
                     AGLGIVLGRGISADWVRVMGGGIVVAGLVLWVGV"
     gene            3050..3382
                     /locus_tag="AC16FV_11005"
     CDS             3050..3382
                     /locus_tag="AC16FV_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR2850616.1"
                     /translation="MAEEMEDELQRLLDTELQAEALVREAELKREKMIRQALEDARMA
                     EQQFEARLPELRASFMEKAEERAVQAVAELARRYEERRAQLRTLAKEREQEAIQAALA
                     LLLDPGRT"
     gene            3384..>3951
                     /locus_tag="AC16FV_11010"
     CDS             3384..>3951
                     /locus_tag="AC16FV_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR2850617.1"
                     /translation="MGAAARYAYLHGRTSVLAARLFSVSQLQSLIQLPLGQETEILQA
                     AGITEFQPGEPTQSPRSREQRLVTVLLYDLVVLARALSGDERGFLLYWAYRFELSNLK
                     TILRGKLNSQDANTIRDQLVDMGPFARLPMEELLRAEDVAELLRQLEGTPFADIARQA
                     RRIFEERHESFALDAAIDRQYFAGLAKRA"
BASE COUNT          772 a         1226 c         1271 g          682 t
ORIGIN      
        1 aggtgcttaa cgcgctgggt gagaactgga ccatccggct cgacgggtat gaagccacca
       61 acttcgggct actgtcgggc aatgcccaat ggcagaagga tatgagcatg aagatcatca
      121 acgccgcgat caaatgcggc gcgaaactcc tgatcctgcc cgagtgcggc cacgcttacg
      181 gcgcgttgcg ctggcagggt gccaacatgt acggcaagcc gttgccgttc aaggtcatgc
      241 acatctccga gtacctcgcg gaaaacctga acaacggcaa gctcaaggtc aagcctgtgg
      301 ccaagtcggt caccttccac gatccctgcc agatttcgcg ccgtggcggc gccacgcgtg
      361 ccggacgcga tgtgctcaag gcgctcggcg tggagctgcg agagatgacc cccaccggcg
      421 acatgaactg gtgttgcggc gggggcggcg gcgtcgtcac gatccaccgc gccgacgagc
      481 tgcgctacaa ggtattcgag atcaagatga agcaggtgaa cgataccggc gccgagatgc
      541 tggtcatgag ctgctccaac tgccgccaga cgttcgacga cgggcaggag catttcaagt
      601 gggacaagac ggcgaacagc ctgctggaaa tggttgcgga caatctagtc gagggataac
      661 caatggccac tcaaacagtc gtagtcgatc cgatcacccg catcgaaggc cacctccgca
      721 tcgaggccaa gactgacggg aacggcgtta tcacccaggc ttcgagcgcc ggcaccatgg
      781 tgcgcggcat cgaaatcatc ctgcgcgggc gcgatccgcg cgatgcttgg gccttcgccc
      841 agcgcatctg cggcgtgtgc acgctggtgc acggcatcgc ctcggtgcgc gcggtcgagg
      901 atgcgttgaa atatgaaatt ccggccaacg cacagctgat ccgtaacctc atgatcggcg
      961 cgcagtacgt gcacgatcac gtgatgcact tctaccacct gcacgcgctc gactgggtgg
     1021 acgtggtttc ggcgctcaag gccgatccga agaagacctc ggaactggcg caggccatca
     1081 gcaactggcc caagtcctcg cccggctatt tcgccgacat gcagaagaag gtgaagaatt
     1141 tcgtcgaagc cgggcagctc ggcattttcg ccaacggtta ctggggacat ccggcgtaca
     1201 agctgccgcc cgaggccaat ctcatggcgg tggcgcatta cctcgaagcg ctggcgtggc
     1261 agcgcgacgt ggtgcagata cacaccattt tcggcggcaa gaacccgcat ccgaatttcc
     1321 tggtcggcgg cacaccggcg gcgatcagcc tgcatccaca gcagcagggt cagggcaagg
     1381 gcccgcatta ccgcggcggg gcaggcgcca cggcggtgaa tatggtcggc ttgcagcgcg
     1441 tgaagaacgt catcgactcg atgcgcgcct tcgtggacca ggtgtacgtg cccgacacgc
     1501 tcgccatcgc cggcttctac aaggactggg gcgcgcaggg cgagggtgtg ggcaatttcc
     1561 tgtgctacgg cgacttcccg tccaagggca ccaacgacgt caacagtttc ctggtgccgc
     1621 gtggcgtgat actcaaccgc gatctctcca agattcacga ggtcgatctc aacgccgagg
     1681 accagattca ggagttcgtc gcgcactcgt ggtacgacta cagcggcggc aaggacaagg
     1741 gtctgcatcc gtacaagggc gagaccaagt tcaattacac cggccccaag ccgccgtacg
     1801 aatacctcga caccgacaag agctattcct ggctcaagtc gccgcgctgg aagggcaagg
     1861 ccgtggaggt cgggccgttg gcgcgtgtgc tgatgctgta cgccaagggc cacaaacaga
     1921 ccaaggaact cgtgcacatg acgctgaagc agctcgatgt cccagtcggc gcgttgttct
     1981 ccacgctcgg ccgcaccgcg gcgcgcacgc tcgaaaccaa gatcttcgtg gacgcgctgg
     2041 aaggctggta caaccagctg atcgccaaca tcaaggccgg cgacgtgaag actttcaacg
     2101 agaaactgtg ggaaccctcg acatggccga aggaggccaa gggcgtgggt tacaccgagg
     2161 cgccgcgcgg cgcgctggca cactggatcg tcatcaagga cggcaagatc gacaactacc
     2221 aggctgtggt gccgagcacc tggaacgccg gtccgcgcga cccaagcgga caggaaggcc
     2281 cgtacgaagc ggcactcaag ggccacaagc tgcacgacgc gaaacagccg gtcgaaatcc
     2341 tgcgcaccat ccacagcttc gatccctgca ttgcctgcgc ggtgcacgtc accgatccgg
     2401 aaggtgagga attgatcaag gtaaaggtca gctaaaggag acatccatga aacgcggata
     2461 ccaaataaca gcggcactct cggcgctttt cctggcgggc acggcgcaag cacacacatt
     2521 cggcgcgcac ggcgcgggat tcgccgccgg cttcgcgcat ccctttatcg gtctcgatca
     2581 tttgctggca atgctggccg tcggcgtgtg ggcggcacag ctcggcgggc gcgccatctg
     2641 gcgcgtgcca ttggcattca tggccatgat gctgctgggc agcgcgctcg ctctcggcag
     2701 tctgccgctt cccgccgtcg aaaccggtgt tgccagctcg atattggtgc tgggcttgct
     2761 catcgcggtg gcggcgcgct tcccgctcgt ggtcagcatg ctgctggtcg gcgccttcgc
     2821 attgtttcac ggccatgccc atggtcagga gctgccacag gctgcttccg catccttata
     2881 tagtctgggc tttctgctcg ccactggcct gttgcatgcc actggcgcgg gtctgggcat
     2941 tgtgctcggt cgtggtattt cagctgactg ggtgcgcgtg atgggcggtg gtatcgtggt
     3001 cgcgggtctg gtcctctggg tgggcgtctg agattttatc gggagcgtta tggccgaaga
     3061 gatggaagat gaattgcagc ggctgctcga caccgagctc caggccgagg cgctggtgcg
     3121 ggaggccgaa ctcaagcgcg aaaaaatgat ccgccaggcg ctcgaggatg cgcgcatggc
     3181 ggagcagcaa ttcgaggcgc ggctgccgga actgcgggcc tcgttcatgg aaaaagcgga
     3241 ggagcgcgcc gtgcaggcag tggcggaact ggcccggcgc tatgaggaac ggcgcgccca
     3301 gctgcgcacg ctcgcaaagg aacgcgaaca ggaggctatt caagcggcgc tcgcgctgct
     3361 gctcgacccg ggacgaacct gacatgggcg ccgccgcgcg ttacgcctat ctccatggcc
     3421 gcacctcggt gctggccgcg cggctttttt ccgtgtccca gctccagagc ctgatccagc
     3481 tgccgctcgg tcaggagacc gaaatcctgc aggccgccgg cattaccgaa ttccagccgg
     3541 gagaaccgac ccaatcgccg cgttcgcgcg aacagcggct tgttaccgtc ctgttgtacg
     3601 atttggtggt gctggcgcgc gcgctgtcag gcgacgagcg cggctttctg ctctactggg
     3661 cctaccgctt cgaactgagc aatctcaaga ccatcctgcg cggcaagctg aacagccagg
     3721 acgccaacac cattcgcgac cagctcgtcg acatgggacc gttcgcgcgc ctgccgatgg
     3781 aggagctgct gcgcgccgag gacgtggccg aactgctgcg ccagctggag ggcacgccgt
     3841 tcgccgatat cgcacgtcag gcgcgacgca tcttcgagga gcgccacgag tcgttcgcgc
     3901 tcgatgccgc catcgaccgc cagtattttg ccggacttgc caaacgcgcg c
//
LOCUS       DBNGZE010000428         2103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_893503, whole genome shotgun sequence.
ACCESSION   DBNGZE010000428 DBNGZE010000000
VERSION     DBNGZE010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_893503"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="AC16FV_11015"
     CDS             complement(<1..216)
                     /locus_tag="AC16FV_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2850618.1"
                     /translation="MTFEKLTLHPSILKAINESGYTVPTPIQAQAIPQALEGHDLMAS
                     AQTGTGKTAAFVLPSLHRLSMPAVKHSR"
     gene            complement(515..727)
                     /locus_tag="AC16FV_11020"
     CDS             complement(515..727)
                     /locus_tag="AC16FV_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850619.1"
                     /translation="MGPGAPVPCRACGKTVKVAFPDWLKSTLPGAAVMIAALFFDSDL
                     MVYGLSLIGLALMIGLHVWLVPLVKE"
     gene            949..1611
                     /gene="nfi"
                     /locus_tag="AC16FV_11025"
     CDS             949..1611
                     /gene="nfi"
                     /locus_tag="AC16FV_11025"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173329.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HIR2850620.1"
                     /translation="MRTRRTQAWPTNISEARAIQESLRGKVLARDRLGKIETVAGIDV
                     GFEKHGTVTRAAVVVLDFPGLTPREQAIARLPTRFPYVPGFLSFREIPAVLAAMKKLH
                     ARPDLILCDGQGLAHPRRFGLACHLGLLLDIPSIGVAKSRLIGTHGEVPERKGGWTAL
                     EDDDEVIGAVLRTRADVKPVYVSIGHRISLATAIDYVLRCTTSYRLPETTRHAHRLAS
                     AV"
     gene            1728..>2103
                     /locus_tag="AC16FV_11030"
     CDS             1728..>2103
                     /locus_tag="AC16FV_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850621.1"
                     /translation="MKKFFCCRNHPVYALAMAVFCGVVTQAQAATTGETSVSLGAEYT
                     SGDYGTSSKTKMWYFPATLRYETDRYMLALTVPYVVVEGTGNVVAAGGMGGMRRRTTS
                     LTSRTESGLGDIERAASHVLAQV"
BASE COUNT          454 a          579 c          648 g          422 t
ORIGIN      
        1 gcgcgaatgc tttaccgcgg gcatgctcag ccgatgcagg gacggcagca cgaatgccgc
       61 ggtcttgccg gtaccggtct gggccgaggc catgaggtca tggccttcga gcgcctgcgg
      121 gatcgcctgc gcctggatcg gggtgggaac ggtgtagccg ctctcgttga tggccttgag
      181 gatggacgga tgcagcgtca gtttttcaaa agtcacgatg tttccttgga ttgcaaatta
      241 aatttcgccc aacttcgttt gtctgggcaa atgatgggcg cgtaagagca aaccgccgtt
      301 tgtcggattt cggcaacagc gttagcacgg atggcgcgaa cacatcgtcg ccaaccatcg
      361 tattgctgaa attccaggga aactgaaacc agaaggtggg tcagagagcg atgaacttat
      421 cggctggacc ggactttact gtccggatcg atcagccggc gcgaaccata caggaatacc
      481 acaagaaaag ccagcacatg tcgcctgcac cgaattactc cttgaccagc ggcaccagcc
      541 agacgtgcaa gccgatcatc aacgccaggc caatcaggct caagccataa accatgagat
      601 cggaatcgaa aaacagggcg gcaatcatga cggccgcgcc tggcagggtg gacttgagcc
      661 agtccgggaa agcgactttg acggtttttc cgcaggcccg acagggaacg ggggcgccgg
      721 gacccaaaaa ggctttacgc aggaacgtga tcgcgggttt tccgcaatga ggacactgat
      781 acattttgct ccaccccgtg tcgctgtgtt gacactctat ttatggttga ttgaaaccgc
      841 cctgtttgat caactcatgc gagcgaggtt gatcgccgga ccagaatata acagccagcg
      901 aaaagacaaa aggcacatta ggcacattca tcagggcggg aatcggtatt gagaacgcgg
      961 cgcacacagg catggccgac caatatcagc gaagcgcgcg cgatccagga gtccttgcgc
     1021 ggcaaggtgc ttgcgcgcga tcggctcggc aagatcgaga cagtcgcggg aatcgatgtc
     1081 ggttttgaaa aacacggaac cgtcacgcgc gcggccgtgg tagtgctgga ttttcccgga
     1141 ctgacaccgc gcgaacaggc catcgcccgc ctgccgacgc gcttccccta tgtcccgggt
     1201 tttctgtcct tccgtgaaat cccggcggtg ttggccgcca tgaaaaagct ccatgccagg
     1261 ccggatctga tcctgtgtga cggacaaggg ctggcacatc cgcggcgttt cgggctggct
     1321 tgtcatctcg gcctgctgct ggatattccc agcatcggcg tggccaagtc gcgactgatc
     1381 ggaacgcatg gcgaagtgcc ggagcgaaag ggtggctgga cggcgctcga agacgatgac
     1441 gaggttattg gcgcggtgtt gcgcacacgc gcggacgtga aaccggtcta tgtgtccatc
     1501 ggacatcgca tcagtcttgc aaccgccatc gattatgtgc tgcgctgcac gacgagctac
     1561 cgcctgccgg aaaccacgcg tcacgcgcat cggctggctt ccgccgtttg acggccgcac
     1621 acttttttga tcggggtcaa cgattcatag tggcataccc gtattatggc gtcagcacgg
     1681 cggttgatat ggcctcacat aattcttcca attcaaggat cacgaccatg aaaaaatttt
     1741 tttgttgtcg gaatcatccg gtttatgcgc tggccatggc agttttctgc ggcgtggtaa
     1801 cgcaggcgca ggcggccact accggcgaaa ccagtgtcag tctgggggcg gaatacacct
     1861 cgggcgatta cgggacttcc agcaaaacca agatgtggta tttcccggcc acgctccggt
     1921 atgaaaccga tcgctacatg ctggcgctga ccgtgcctta tgtcgttgtc gagggcaccg
     1981 gcaatgtcgt agctgcgggc ggcatgggtg ggatgcgtcg caggacgacg agcctgacga
     2041 gcaggactga atccggactg ggcgacatcg agcgggcggc ttcgcatgtg cttgcgcagg
     2101 tcg
//
LOCUS       DBNGZE010000429         6773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_338734, whole genome shotgun sequence.
ACCESSION   DBNGZE010000429 DBNGZE010000000
VERSION     DBNGZE010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_338734"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="AC16FV_11035"
     CDS             complement(<1..1389)
                     /locus_tag="AC16FV_11035"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0097216 - guanosine tetraphosphate binding
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein TypA/BipA"
                     /protein_id="HIR2850622.1"
                     /translation="MIQKLRNIAIIAHVDHGKTTLVDKLLQQSGTFAAHQQVGTRVMD
                     SNDLEKERGITILAKNTAIRWNDYRINIVDTPGHADFGGEVERVLSMVDSVLLLVDAM
                     DGPMPQTRFVTRKAFAHGLHPIVVINKIDRPGARPDWVLNQTFDLFDKLGATDAQLDF
                     PVVYASALNGYASLDPTATSGDMTPLFETIIKHVPAPDVDVDGPFQMQVSSLAYSSYV
                     GAIAVGRIKRGRVKPGQNVTVVEREGNTRNAKILQVLGFMGLERTEVPEASAGDIVAI
                     TGIEHPHISDTFCDPATVEALPPLTVDEPTVTMTFEVNNSPFAGQDGKFVTSRQIRER
                     LDRELIHNVALRVEDTDSPDKFRVSGRGELHLGILIENMRREGYELGVSRPQVIVKMV
                     NNEPHEPFESVTVDIEQQHQCVVMEKMGLRRGDLQNMEPDGKGRVRLEYIIPSRGLIG
                     FRNEFLTATQGSG"
     gene            complement(1474..2352)
                     /gene="hslO"
                     /locus_tag="AC16FV_11040"
     CDS             complement(1474..2352)
                     /gene="hslO"
                     /locus_tag="AC16FV_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR2850623.1"
                     /translation="MTTGDSLQRFIFENAPIRGEIVHLDATWQAVLERRQYPPRVRDV
                     LGELMAAAALRTSTLKFDGRLIMQVQGNGPVSLLVVECTSDRTMRAIAQWAGEVPDAP
                     LATLVGNGRLAVTIDPLKGKERYQAIVNLEGLTVAEAFENYFARSEQLDTRLWLASDP
                     NQAAGMLLQRLPGDSLTDDDDAWGRAVHLGSTITREELLALPVREIIHRLYHEEDIRL
                     FSRVPVSFRCSCSRDRVESVLRMLGHGEIHSILAEQGTVRVDCEFCGNRYEFDRIDAE
                     QLFASLHSAPASHTRH"
     gene            complement(2435..2815)
                     /gene="trxA"
                     /locus_tag="AC16FV_11045"
     CDS             complement(2435..2815)
                     /gene="trxA"
                     /locus_tag="AC16FV_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR2850624.1"
                     /translation="MATVEVTQENFNQVVSGNNMVIVDFWAPWCGPCRSFAPAYEAAS
                     EKHPDVVFAKVNTEEQQALAGYFQIRSIPTLMIFREKIIIFAQPGALPASALEQVIEK
                     AKALDMNEVRREIEKEQASAANKG"
     gene            complement(3014..3733)
                     /gene="phoU"
                     /locus_tag="AC16FV_11050"
     CDS             complement(3014..3733)
                     /gene="phoU"
                     /locus_tag="AC16FV_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR2850625.1"
                     /translation="MEQMHTHISRQFSAELEDIRSRVLQMGGLVEQQIEQAIEALVKG
                     DTALGENVIMHDTQVNKMEVTIDEECNQIIARRQPAASDLRLVVAVIKTITDLERIGD
                     EAEKIARMAVRLAGEERPKNNYAEIQALGGYVRHMLHEALDAFARLDIEAAVRVARED
                     IKVDQKYEGTMRQLITYMMEDPRTISRVLNVIWAARALERIGDHACNICEYIIYLVKG
                     KDVRHTSLDQVEREILGKDRR"
     gene            complement(3906..4673)
                     /gene="pstB"
                     /locus_tag="AC16FV_11055"
     CDS             complement(3906..4673)
                     /gene="pstB"
                     /locus_tag="AC16FV_11055"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017349358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR2850626.1"
                     /translation="MANDINGKIQVRDLNFYYGKYQALKNIGLDVATNMVTAFIGPSG
                     CGKSTLLRTFNRMYELYPGQRATGEILLDGKNVLAPGTDLFRLRARVGMVFQKPTPFP
                     MSIYENIAFGVRLYEKLSRSEMDDRVEWAITQAALWGEVKDKLRQSGMSLSGGQQQRL
                     CIARAIAVKPEVMLLDEPASALDPISTLKIEELIYELKKNYTIVIVTHNMQQAARVSD
                     FTAFMYLGEAVEFGDTNTIFTNPSKKQTEDYITGRYG"
     gene            complement(4673..5518)
                     /gene="pstA"
                     /locus_tag="AC16FV_11060"
     CDS             complement(4673..5518)
                     /gene="pstA"
                     /locus_tag="AC16FV_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR2850627.1"
                     /translation="MDLRYFRRRTVNIVNLTGSLLATVFGLFWLFWILWTTLSYGLAA
                     FDWNLFHQNTPAPGATGGLANAIVGSLIMVGLATVIGTPVGILAGTYLAEYGGRSWVA
                     ATIRFINDILLSAPSIIIGLFVYEMVVIRMGHFSALAGAIALAIIMLPVVIRTTEEML
                     RLVPNPLREAALALGAPRWKMISLVAYRAALSGILTGVLLAVARIAGETAPLLFTALN
                     NQFWSTDLTQPMANLPVVIFQFAMSPYSEWHTLAWAGSLLITLSVLALSILARTLLRK
                     ARLEH"
     gene            complement(5522..6493)
                     /gene="pstC"
                     /locus_tag="AC16FV_11065"
     CDS             complement(5522..6493)
                     /gene="pstC"
                     /locus_tag="AC16FV_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR2850628.1"
                     /translation="MNIGHSHISDTISGRQRLMDRFFHALTTGSAWLVLVILGGIIVS
                     LFVGGWQALVEFGPKFLVTDEWNPVTEKFGALVPIYGTLVSSLIALLIAVPISFGIAV
                     FLTEVAPGWMRRPIGTAVELLAAIPSIIYGMWGLFVFAPVMSQYVQPWLQTQFGDIAF
                     IGTLFQGAPLGIGMLPAGIILGIMIIPFITALMRDVFLTTPKFLKESAYGVGATHWEV
                     VWNVVLPYTRVGAVGGIFLGLGRALGETMAVTFVIGNTHRMSASLFEPGKSIASTLAN
                     EFNEAVGDMHTSALIALGLVLFMITFIVLALAKLLLLGLESRDSAKS"
     gene            complement(6561..>6773)
                     /locus_tag="AC16FV_11070"
     CDS             complement(6561..>6773)
                     /locus_tag="AC16FV_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIR2850629.1"
                     /translation="LVYKKQEKTETARQVLKFFDWAYHDGGKMAEGLDYVPMPASVVK
                     LVEDTWKTEIKDASGKAVWTDSMVQK"
BASE COUNT         1253 a         2016 c         2146 g         1358 t
ORIGIN      
        1 gcccgagccc tgggtggcgg tgaggaactc gttgcgaaag ccgatgaggc cgcgcgacgg
       61 aatgatgtat tcgaggcgca cacgaccctt gccatccggt tccatgttct gcaggtcgcc
      121 gcggcgcagt cccatcttct ccatcaccac gcactggtgc tgctgttcga tatcgaccgt
      181 gaccgactcg aacggctcgt gtggttcatt gttgaccatt ttcacgatca cctgcgggcg
      241 cgacacgccg agctcgtagc cctcgcggcg catgttctcg atgaggatgc caagatgcag
      301 ttcgccacgg cccgagacgc ggaacttgtc cgggctgtcg gtatcttcca cgcgcagcgc
      361 gacgttgtgg atcagctcgc ggtcgaggcg ctcgcggatc tggcggctgg tgacgaactt
      421 gccgtcctgg cccgcgaacg gcgagttgtt cacctcaaag gtcatggtga cggtcggctc
      481 gtccacggtc agcggcggca acgcctcgac cgtggccgga tcgcagaagg tgtcggagat
      541 gtgcggatgc tcgatgccgg tgatggcgac gatgtcgccg gcactggcct ccggcacctc
      601 ggtgcgctcc aggcccatga agccgagcac ttgcaggatc ttggcgttgc gggtgttgcc
      661 ctcgcgctcc accacggtga cgttctggcc gggcttgacg cggccgcgct tgatgcggcc
      721 gacggcgatg gcgccgacgt agctcgagta cgccaggctc gacacttgca tctggaacgg
      781 tccatcgacg tccacgtccg gcgccgggac atgcttgatg atggtttcga acagcggcgt
      841 catgtcgccc gaggtggcgg tcgggtcgag gctggcgtag ccgttcaggg ccgaggcgta
      901 caccaccggg aaatcgagtt gtgcgtcggt cgcgccgagc ttgtcgaaca ggtcaaaggt
      961 ctgattcagc acccagtccg ggcgcgcgcc gggtcggtcg atcttgttga tgaccacgat
     1021 cgggtgcagg ccgtgggcga aggccttgcg cgtgacgaag cgcgtctgcg gcatcggccc
     1081 gtccatcgcg tccaccagca gcagcaccga gtccaccatc gacagcacgc gctcgacctc
     1141 gccgccgaaa tcggcgtggc ccggggtgtc gacgatgttg atgcggtaat cgttccagcg
     1201 gatcgcggtg ttcttcgcca ggatggtgat gccgcgttcc ttctccagat cgtttgaatc
     1261 catgacgcgc gtgcccacct gctggtgcgc ggcaaaggtg ccggactgtt gcaggagctt
     1321 gtcgacgagc gtggtcttgc cgtggtcgac gtgggcgatg atggcgatgt tgcgcagttt
     1381 ctgaatcatt ggatttaccg tttacgtgag ggtcacagcc aaaaggccgc gcattataca
     1441 cgtttacctg ggaaaaaacc taccgcaggt aggttagtgg cgcgtatgcg aggcgggggc
     1501 gctgtgcagg ctggcaaaca gctgttccgc gtcaatccgg tcgaattcgt agcgattgcc
     1561 gcaaaactcg cagtccaccc ggaccgtccc ctgttccgcc aggatcgaat ggatttcacc
     1621 gtgtccgagc atgcgcagca cggactccac ccggtcgcgc gagcaggaac aacggaagct
     1681 gactggcacg cgtgagaaca ggcgtatgtc ctcttcgtga tacaggcggt ggatgatctc
     1741 gcgcacgggc agggccagca actcttcccg agtgatggtc gagcccaagt gcacggcgcg
     1801 accccaggcg tcatcgtcgt ccgtcaacga gtcgccgggc agccgttgca agagcatccc
     1861 ggctgcctgg ttgggatcgg aggcaagcca caggcgtgtg tcgagttgct ccgagcgggc
     1921 gaaatagttt tcaaacgcct cggccacggt gagcccctcg agattcacga tggcctggta
     1981 gcgttccttg cccttcaacg gatcgatggt gacagccagc cgcccgttgc cgacgagcgt
     2041 cgccagcggc gcgtccggca cttcgccggc ccactgcgcg atagcgcgca tggtgcggtc
     2101 gctggtgcac tcgaccacca gcaaactgac cggcccgttg ccctgtacct gcatgatgag
     2161 ccggccgtcg aatttgagcg tggaggtccg cagtgcagcg gcggccatca gctcgcccag
     2221 cacgtcgcgc acgcgcggcg gatactgacg ccgttcaagc accgcctgcc aggtggcgtc
     2281 gagatgcacg atctcgccac ggatcggggc gttttcgaag atgaagcgtt gcagcgaatc
     2341 gccggtggtc atgactgtcc cttcattctc agtataacct ccgggcatac cggtgggtgg
     2401 aatggcggga aaacacggcg tttggagggg agcgtcagcc tttgttcgcg gcgctggcct
     2461 gttccttctc gatctcgcgg cggacttcgt tcatatccag cgccttggcc ttctcgatca
     2521 cctgttcgag ggcggaggcc ggcagggcgc cgggttgcgc gaagatgatg atcttttcgc
     2581 ggaaaatcat aagcgtcggg atcgagcgga tctggaagta gcctgccagc gcctgttgtt
     2641 cctcggtgtt gaccttggcg aacacgacat ccgggtgttt ttccgaggcc gcttcgtacg
     2701 cgggcgcgaa cgaccggcag ggtccgcacc acggcgccca gaaatccacg atcaccatgt
     2761 tgttgccgct tacgacctga ttgaaatttt cctgcgtaac ttcgacggta gccactttat
     2821 ctccagcgga catcgcgcgg atgcccatga cgttggggtt gaacgaatat tataccgata
     2881 ccacaagatt gctctttgaa ttcgaggatg ggggcgggac ggacaaaatc aagttactgc
     2941 cgcgaggaca tataaaaacc ggacgcttga tctgaaagga caaaatgtcc gaaccgcgat
     3001 ggtgcgggat gcgtcagcgt ctgtctttcc ccaggatttc ccgttccacc tgatcgaggc
     3061 tggtatggcg cacgtccttg cccttgacca gatagatgat gtattcacag atgttgcagg
     3121 catggtcgcc gatacgctcg agcgcgcgcg ccgcccagat gacgttcagc acacgtgaaa
     3181 tggtgcgtgg gtcttccatc atgtaggtaa tgagctggcg catggttccc tcgtacttct
     3241 ggtccacctt gatgtcctcg cgcgccaccc tcaccgccgc ttcgatatcg aggcgcgcga
     3301 atgcgtcgag tgcctcgtgc aacatgtgcc gcacatagcc gcccagcgcc tggatctcgg
     3361 cgtaattgtt cttggggcgt tcctcgcccg ccagccgtac cgccatgcgc gcgattttct
     3421 ccgcctcgtc gccgatgcgc tccagatcgg tgatggtctt gatgacggcc acgacaagcc
     3481 gcaggtcgct ggccgcaggc tgacgccggg cgatgatctg gttacattcc tcgtcgatgg
     3541 tgacttccat cttgttgacc tgggtgtcgt gcatgatgac gttttcaccc agcgccgtgt
     3601 cgcctttgac gagagcctcg atggcctgtt cgatctgctg ctccacgagc ccgcccattt
     3661 gtagcacgcg cgagcggata tcctccagtt cggcgctgaa ttggcgggag atatgggtgt
     3721 gcatttgttc catggctgct cctatgcaag ctccgaaaaa cggcggagtt tgtctttata
     3781 cttcgttgcg gctggtctgc gctgctcaca tactgtcatg tatgctgcgc tgctcgcctg
     3841 cgccgcgcct cgtctaaagt caaacttctt gtttttcgga gttgccactg ttggcgtacg
     3901 gattattagc cgtaacgacc ggtgatgtaa tcttccgtct gcttcttcga cggattggtg
     3961 aagatggtgt tggtgtcgcc gaactccact gcctcgccga ggtacatgaa agccgtgaag
     4021 tcggatacgc gcgcggcctg ctgcatgttg tgcgtgacga tcacgatagt atagttcttt
     4081 ttgagttcgt agatcagttc ttcgatcttc agggttgaga tcggatcgag cgccgaggcc
     4141 ggttcgtcga gcagcatcac ttcaggcttc acggcaatgg cgcgcgcgat gcatagccgc
     4201 tgctgctggc cgccggagag gctcatgccg ctctggcgca gtttgtcctt gacctcgccc
     4261 cataacgccg cctgcgtgat cgcccactcc acgcgatcgt ccatctccga acgcgacagc
     4321 ttttcataca ggcgcacgcc gaaggcgatg ttctcgtaga tcgacatggg aaacggcgtt
     4381 ggtttctgga ataccatgcc gacccgcgcc cgcagccgga acaggtccgt ccccggtgcg
     4441 aggacgtttt tgccgtcgag caagatctcg ccggtggcgc gctgacccgg atagagctca
     4501 tacatgcggt tgaaggtgcg caacagcgtg gatttgccgc aacccgaggg gccgataaag
     4561 gccgtgacca tattggtggc gacatccagg ccgatattct tgagcgcctg gtacttgccg
     4621 taatagaagt tcaggtcgcg aacctgaatc ttgccgttga tgtcgtttgc catcagtgct
     4681 ccagtcgcgc cttgcgcagc agggttcgcg ccaggatgct caacgccagg acgctcaggg
     4741 taatcaggag tgaaccggcc caggcgagcg tgtgccattc cgagtatggg ctcattgcga
     4801 actggaagat caccaccggc aggttggcca tcggctgcgt caggtcggtg ctccagaact
     4861 ggttgttgag cgcggtgaac aggagcggcg cggtttcgcc ggcgatgcgc gcgactgcca
     4921 gcagcacacc ggtgaggatg ccggagagcg ccgcgcggta ggccacgagc gagatcattt
     4981 tccagcgcgg cgcgcccagt gccagtgccg cttcgcgcag cgggttcggc accagccgca
     5041 gcatctcctc ggtggtgcgg atcaccaccg gcagcatgat gatggcgagc gcgatcgcgc
     5101 cggcgagcgc ggagaaatgt cccatgcgga tcacgaccat ctcgtaaacg aaaagcccga
     5161 tgatgatcga cggcgcggac agcaggatgt cgttgatgaa gcggatggtc gcggccaccc
     5221 agctgcgacc gccgtattcc gcgagatagg tgccggcgag gatgccgacg ggcgtgccga
     5281 tcacggtcgc cagcccgacc atgatcaggc tgccgacgat ggcattggcc agcccgccgg
     5341 tggcgccggg ggcgggcgtg ttctggtgaa acaggttcca gtcgaaggcc gcgaggccgt
     5401 agctcagggt ggtccagagt atccagaaca gccagaacag gccaaagacg gtggccagca
     5461 gcgaccccgt gagattcacg atattgacgg tacgtcgccg gaagtagcgc agatccatgg
     5521 gtcaactttt ggcgctgtcg cgcgattcca gcccgagcag cagcagcttg gccaacgcca
     5581 gcacgatgaa ggtgatcatg aacagcacga ggccgagcgc gatcagcgcg gaggtgtgca
     5641 tgtcgcccac ggcttcgttg aattcattcg ccagcgtcga ggcgatggac tttcccggct
     5701 cgaacagcga ggcgctcatg cggtgggtgt taccgatgac gaaggtcacg gccatggttt
     5761 cgccgagggc acgtccgagg cccaagaaaa tgcctccgac cgcgcccacg cgcgtgtacg
     5821 gcagtaccac gttccagacg acctcccagt gtgtggcccc gacgccgtag gccgactcct
     5881 tgagaaactt gggcgtggtg agaaacacat cgcgcatgag cgcggtaatg aaggggatga
     5941 tcatgatgcc gaggatgatg ccggcgggca gcatgccgat gccgagcggc gcaccctgga
     6001 acagggtgcc gatgaacgcg atgtcgccaa actgtgtctg cagccaaggc tgaacatact
     6061 gcgacataac cggtgcgaac acgaacagtc cccacatgcc atagatgatg ctggggatgg
     6121 ccgcgagcag ttcgaccgct gtgccgatcg ggcgccgcat ccatcccggc gcgacctcag
     6181 tgagaaacac ggcgatgcca aaacttatcg gtaccgcgat caacagcgcg atcagggagc
     6241 tgaccagcgt tccgtatatc ggcacgagcg cgccgaattt ttcggtcacc ggattccatt
     6301 catcggtcac aaggaacttc ggaccaaact cgaccagggc ctgccagcca ccgacgaaca
     6361 gggatacgat gatgccgcca aggatcacca gcaccagcca cgccgaaccg gtggtcaagg
     6421 catggaagaa acggtccatg aggcgttgcc gcccgctgat ggtgtcggaa atatgtgaat
     6481 ggccgatgtt cattattgtg taaagagaaa gcggggtcct gatggacccc gccgcacaca
     6541 gcctataagt gaagcaaatt ctacttctgg accatgctgt cggtccagac agccttgcca
     6601 ctcgcgtcct tgatctcggt tttccatgtg tcttcaacca gttttacgac gctggccggc
     6661 atcgggacgt aatcgagtcc ctcggccatc ttgccaccgt cgtgataggc ccagtcgaag
     6721 aacttcagca cctgccgggc ggtctcggtt ttttcctgtt tcttgtaaac cag
//
LOCUS       DBNGZE010000430        10705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_476859, whole genome shotgun sequence.
ACCESSION   DBNGZE010000430 DBNGZE010000000
VERSION     DBNGZE010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_476859"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="AC16FV_11075"
     CDS             complement(<1..799)
                     /locus_tag="AC16FV_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850630.1"
                     /translation="MNKRHYKLLTELLTQPTAPFREEHVIVVAQRVLEQAGIPFFSDP
                     VGNIVVGCASRADYHRLVREKSGEPLRLLIAHMDHPGFHGVRWMSDTRLKVKWHGGSP
                     VRHLTGSRVWLATAQGHWGEGKLSRARLIKSKHAIDTAEVRVNGAGDGARSKADTLHG
                     GFRFRAPVWRAGKKIYAKAADDLVGVFAILSTALDLHKRNRGKKSPPFFGLLTRAEEV
                     GFIGAIGHFELGWLSGARRPVIAVSLETSRTLPGALIGKGPVVRLGDR"
     gene            924..1118
                     /locus_tag="AC16FV_11080"
     CDS             924..1118
                     /locus_tag="AC16FV_11080"
                     /inference="COORDINATES: protein motif:HMM:NF033233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR2850631.1"
                     /translation="MTMLTAIIVIGMLATAGALFSGIVSMVQGGDFDQHHSHQLMFAR
                     VGLQGITLLFLFLALLVVAR"
     gene            1251..1616
                     /locus_tag="AC16FV_11085"
     CDS             1251..1616
                     /locus_tag="AC16FV_11085"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2850632.1"
                     /translation="MHHPLVFDVTEQDFTENVLEASQHRPILVDFWADWCPPCRALTP
                     VLEKVARGLHGEVLLAKVEVDENMRLAGRYHLRGFPTVILFVRGEEVGRFSGARSEQQ
                     VRAFLSDHLAGTSTAAASP"
     gene            complement(1623..2495)
                     /locus_tag="AC16FV_11090"
     CDS             complement(1623..2495)
                     /locus_tag="AC16FV_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850633.1"
                     /translation="MRSTDMSRGCSATYVLLIALTVLLVLPATGADEARIIQLKHRPA
                     SEIMPLIRPLLGPDDVLNGMDFRLIIRTSDKNLKEVERILAQVDVARQRLRIIVEQAI
                     ADDRTTQSQSVTGEARIGDKVRIILPAKPPENDGLAVQKDGLRYAANRRTTTASNANT
                     QTVMTLDGQRSYIRVGQSVPYVKKILALSRRQLVLVQDVELQDVTTGFEVLPRVRGDR
                     VLVEITPHLSMLRNPTIGLADYQELSTTVETKLGEWLDLGEILGNRGEVERAILESAR
                     TTAGERRTVRLKIE"
     gene            complement(2482..2949)
                     /locus_tag="AC16FV_11095"
     CDS             complement(2482..2949)
                     /locus_tag="AC16FV_11095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR2850634.1"
                     /translation="MRLRIIPILTSFIVLALLTGCGHTPVEESAPALGSRAADHALAM
                     VGKPYRYGGNTPKGFDCSGLVQYSYGRVGVKLPHGTRNLLHISRPISRDKLQRGDLVF
                     FTQEGRKSSHVALYLGDDRFVHSPSGGKNVYVAGFSDLYWQRHFTEARRLEID"
     gene            complement(2965..3480)
                     /locus_tag="AC16FV_11100"
     CDS             complement(2965..3480)
                     /locus_tag="AC16FV_11100"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7931 domain-containing protein"
                     /protein_id="HIR2850635.1"
                     /translation="METAKNPPATETAAQRRIIRDRAEVSDFLAMLAENATREIVIFA
                     PRLDGAFFNTSRFARALASFAARHRQNLARIVVEDTEQTLRDNDRLVGLCRRLSDFIH
                     MQQVAEEHLGIREMFMVVDHHGYLYQQDTAKPDCVAHPHDGHEAVLLTRRFSELWDRS
                     EPIVALRATGL"
     gene            complement(3616..5001)
                     /gene="purB"
                     /locus_tag="AC16FV_11105"
     CDS             complement(3616..5001)
                     /gene="purB"
                     /locus_tag="AC16FV_11105"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR2850636.1"
                     /translation="MPLNSLTALSPLDGRYQTKTAELRPLFSEYGLIRHRVLVEIRWL
                     IALGEHKKIKEVAPFSSATQKQLNAIVADFSEADARRVKEIEATTNHDVKAIEYFLKE
                     KTRDNKEIARCTEFIHFACTSEDINNLAYALMLKQSRDGVLLARMDKLIAAMTDLVNR
                     HAEQPMLARTHGQPASPTTLGKEMANFAYRLKRARKQMADVPILGKINGAVGNYNAHL
                     AAYPEVDWPAFAEAFVEKLGLDWNPYTTQIEPHDYIAELLDAAARFNTILIDFNRDVW
                     GYISLGYFKQKVIKGEVGSSTMPHKVNPIDFENSEGNLGLANALFHHLSEKLPVSRFQ
                     RDLTDSTVLRNLGVGFGYMLLACDSCLKGIGKLGVNAERLRQDLDQNWEVLAEPVQTV
                     MRRHGVEQAYEKLKDLTRGKGGITRESMQAFIRTLDIPDNAKKRLLALTPDTYTGNAA
                     MQARNLAKIKI"
     gene            complement(5099..6199)
                     /gene="mnmA"
                     /locus_tag="AC16FV_11110"
     CDS             complement(5099..6199)
                     /gene="mnmA"
                     /locus_tag="AC16FV_11110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR2850637.1"
                     /translation="MKVVVGISGGVDSAVAALLLKRQGHEVTGLFMKNWEEDDTAGHC
                     GAAEDLKIAQRVCDHLGIPLKTVNFSAEYWDRVFSYFLSEYRAGRTPNPDVLCNKEIK
                     FRAFLDHALTLGADRIATGHYARADQHAGRFRLLKARDGNKDQSYFLYLLGQEALAHA
                     LFPVGDMTKTEVRRLAREAGLPNYDRQDSTGICFIGERDFKSFLARYLPAQPGEMRTL
                     TGALKGKHDGVMYYTIGQRHGLGIGGEGEAWYVVGKDVANNVLYVEQGEQHPALFSEA
                     LIAGQLHWIGAEPFPWPRKLRAKTRYRQPDQDCTVTPHDKGLRVTFDHPQRAITPGQS
                     VVFYDGDECLGGGIIEDAMPATHAVEMHSAQR"
     gene            complement(6196..6648)
                     /locus_tag="AC16FV_11115"
     CDS             complement(6196..6648)
                     /locus_tag="AC16FV_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR2850638.1"
                     /translation="MIWKPNVVVAAIIERDGKFLLVEEKADGRLVLNQPAGHLDEGES
                     LMQAAVRETLEETAWHFTPEALLGVYRWPHPTKDITYLRFAFIGHVTSHEENRALDHD
                     IVRALWLTPEEIRAERARHRSPQVERCLNDYLAGRRYPLELLAEYKDR"
     gene            complement(6944..7909)
                     /gene="thiL"
                     /locus_tag="AC16FV_11120"
     CDS             complement(6944..7909)
                     /gene="thiL"
                     /locus_tag="AC16FV_11120"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR2850639.1"
                     /translation="MNEFELIRTFFARQDVARPDVAAGIGDDAALLQPPAGQQLAVTS
                     DLLVSGVHFLPDVDPFSLGHKALAVNLSDLASMGAEPAWFMLNLALPKADANWLQLFC
                     RGMFELAREHKVQLVGGDTSRGPLTIAIEAHGFVPREQALRRSGAKAGDRIYVTGTLG
                     DAALALRHRLGGIRLPERDLAAVADRMDRPTPRVREGMMLRDIAHSAIDISDGLLADL
                     GHILEMSQVGARISLDKIPVSPVCRSHIRETGWDMVLANGDDYELCFTVPEKNIAALE
                     KLHPACGFHHIGTIEAEPGLRIFDESERPYQPQQSGHDHFAGKTK"
     gene            complement(7918..8358)
                     /gene="nusB"
                     /locus_tag="AC16FV_11125"
     CDS             complement(7918..8358)
                     /gene="nusB"
                     /locus_tag="AC16FV_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR2850640.1"
                     /translation="MVKGSRHKARRAAVQALYQWQLTEQAPREIEAHFILDHEMNGVD
                     LDYFHHLVREIPLHLHELDDHISPHLDRKLNDVDPVELACLRIGTYEFEFHPEIPYKV
                     ILNEAVELAKTFGAEHGHKYVNAILDKIATGLRSREIKAESSPA"
     gene            complement(8369..8845)
                     /gene="ribH"
                     /locus_tag="AC16FV_11130"
     CDS             complement(8369..8845)
                     /gene="ribH"
                     /locus_tag="AC16FV_11130"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR2850641.1"
                     /translation="MNDVKSIDGDLIARSARFGIALGRFNSFIGERLLEGAIDTLVRH
                     GAEAANIEVVRVPGAFEMPLALKTMAASGKYDALIALGAVIRGETPHFEYVAGECAKG
                     VSQVGMQFDLPVAFGVLTVDTIEQAIERAGTKSGNKGVDAALTAIEMVNLLKKLRV"
     gene            complement(8842..10014)
                     /gene="ribBA"
                     /locus_tag="AC16FV_11135"
     CDS             complement(8842..10014)
                     /gene="ribBA"
                     /locus_tag="AC16FV_11135"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR2850642.1"
                     /translation="MPISPITEIIDEIKRGRMVVLMDDEDRENEGDLVMAGAHAAASD
                     INFMARYGRGLICLTLTQERCRQLQLPLMVSDTHHRRSTNFTVTIEAAEGVTTGISAA
                     DRARTIQVAVKRGAKPSDLVQPGHVFPLMAQRGGVLARAGHTEAGVDLARLAGFEPAS
                     VICEIMNEDGTMARLPELEKFAQQHGLKIGTIADLIRYRTEHESTIQRVGECTMPTEF
                     GDFRVLAYHDDIGDSVHLALVRGDIRRDRPTLVRVHMQESLLDLVTTAHRAGSWSLHE
                     ALAHVAAEGAGVVVILQQEEKPADLVQRIRHFQGQGKALESGAGKRGHQEMRTYGLGS
                     QILADLGVSKMRVLGHAMKAPGLSGFGLEIVEYIENDPAQLSSTHRKHNKEKKVSG"
     gene            10253..>10705
                     /locus_tag="AC16FV_11140"
     CDS             10253..>10705
                     /locus_tag="AC16FV_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2850643.1"
                     /translation="MSAKANKADKLQSTVIISGRFSERRAQHATQPAEESSAEPLEPG
                     SMVGRYLTLSRIGVGGMGIVYRAHDTELNRTVALKVLPPKFSQYPEYLKRFHTEAQAQ
                     ARLNSPHVVTLYELMDHPVGQVLVLEYVEGETLESRLRHYGPLSVPEAV"
BASE COUNT         2026 a         3169 c         3240 g         2270 t
ORIGIN      
        1 gccggtcgcc cagacgcacc accggacctt tgccaatgag cgcacccggt agagtgcgcg
       61 aggtttccag actcacggca atcaccggac ggcgcgcgcc ggagagccaa cccagttcga
      121 agtgaccgat ggcgccgatg aagccgactt cctccgcgcg cgtcagcaat ccgaagaacg
      181 gtggtgattt tttaccgcgg tttcttttat gcagatcgag cgcagtgcta aggatggcga
      241 aaacgccgac gagatcgtcc gccgccttgg cgtagatttt cttgccggca cgccataccg
      301 gggcgcggaa cctgaaaccg ccatgtagcg tatccgcctt cgatcgcgcg ccatcaccgg
      361 cgccgttgac acggacctcg gcggtgtcga tagcgtgttt ggacttgatc agccgcgccc
      421 ggctcagctt gccctcaccc cagtgaccct gcgccgtcgc cagccagaca cgacttccgg
      481 tgagatgcct gacaggcgaa ccgccgtgcc atttgacctt gagccgcgtg tccgacatcc
      541 agcgcacgcc gtggaatccg ggatggtcca tgtgcgcgat gagcaggcgc aacggttcac
      601 cgcttttttc acgcactagg cgatggtaat cggcgcgcga ggcgcaaccg acgacgatgt
      661 ttccaacggg gtcgctgaaa aaaggaatac ccgcctgttc caacacgcgc tgcgcgacca
      721 cgattacgtg ctcttcacga aagggagcgg taggctgggt caggagttcg gtcaggagtt
      781 tgtaatgacg cttattcatg gcgcgcgact atagcttttc catcggtaaa aacaacagga
      841 ttgatgcaca tcaaggcagc gtgcgcgtgt gagccgaaaa taaaatcgtt taacggcagc
      901 ctttcgtcag tagggggtat ctcatgacca tgctcaccgc gattattgtt atcggaatgt
      961 tggcgactgc aggcgctttg ttttcaggca tcgtctctat ggtgcaaggt ggtgatttcg
     1021 accagcatca cagccaccag ctgatgttcg cccgtgtcgg cctgcaaggc atcacgttgt
     1081 tgtttttgtt tcttgccttg ctcgtcgtcg cccggtagtc gtcgaattat ctgtcttgaa
     1141 tcgccctcac tgccagggag agggcgtgtt tttgattggc tgtacatttg ccggtaaaat
     1201 agcgcgactt tttaccggca aaactttccc ttcgttttcg aaaagaagaa atgcatcacc
     1261 ctctcgtttt tgatgtcacc gagcaggatt tcaccgaaaa cgtgctggaa gcctcgcaac
     1321 acagacccat cttggtggat ttctgggccg attggtgtcc accttgccgc gcgctaacgc
     1381 cggtactgga gaaagtcgcc cgcggtcttc atggtgaagt gctgttggcc aaggtggaag
     1441 tggatgaaaa catgcggctg gccgggcgtt accatttgcg tggctttccc actgtcatcc
     1501 tgtttgtacg gggcgaggaa gtcgggcgct tcagtggagc gcgttcggaa caacaggtac
     1561 gcgcttttct ttcagaccac cttgccggaa cctcaacggc ggccgccagt ccctgaggtt
     1621 ggttattcga tcttgagtct gacggtgcgg cgttcgcccg cggtcgtgcg ggcgctttca
     1681 aggatggcgc gctcaacctc gccgcggttg cccagaattt cccccaggtc cagccactcg
     1741 cccagctttg tttcgactgt cgttgacagt tcctggtaat cagcgaggcc gatggtcgga
     1801 ttcctcagca tggacaggtg cggtgtgatc tcgaccagta cccggtcgcc gcgcacgcgc
     1861 ggcaatacct cgaagccggt cgtgacatcc tgaagctcga catcctgcac cagcacgagc
     1921 tgacgccggc tgagtgccaa tattttcttt acatatggaa ccgactggcc gacgcggata
     1981 taagagcgtt gtccatccaa cgtcataaca gtctgggtgt tggcgttact ggcggtggtt
     2041 gtgcggcgat tcgccgcgta gcgcagaccg tctttttgca cggccagccc atcattttcg
     2101 ggaggcttgg caggcagaat gatacggacc ttgtcgccaa tccgcgcttc tcccgtaacg
     2161 gactgggatt gagttgtacg atcgtcggcg attgcctgtt ccacgatgat acgcaagcgt
     2221 tggcgcgcta cgtccacctg cgccaggatg cgctcgactt ctttcaggtt cttgtccgat
     2281 gtgcggatga tcaggcgaaa atccatgcca ttcagaacgt cgtccggacc cagcaaagga
     2341 cgaatcaggg gcatgatttc gctcgcgggt cggtgcttca gctggataat acgggcttcg
     2401 tcagcacccg tggcgggcag gacgagcagg acagtgagag caatcaacag aacataagtg
     2461 gcggaacagc cgcgcgacat gtcagtcgat ctcaaggcgt cgggcttccg tgaaatgacg
     2521 ttgccaatac agatcgctga atccggcgac gtacacattt ttgccgccag aaggggagtg
     2581 cacgaaacga tcgtccccca ggtacagcgc cacatgcgaa gatttcctgc cctcctgggt
     2641 aaagaacacc aaatcgccgc gctggagttt gtcgcgcgag atggggcggg atatatgcag
     2701 caggttgcgt gtgccatgcg gcagtttcac cccgacccgg ccataactgt attgcaccag
     2761 gccgctgcaa tcgaaacctt tcggcgtgtt gccgccgtag cgatagggtt tgcccaccat
     2821 ggcgagggca tgatcggccg cccggctgcc cagcgccggt gcggattctt ccacgggcgt
     2881 gtggccgcat ccggtcagca aggccagaac gataaaactt gtcagaatcg ggattattcg
     2941 aagccgcatt ggtattttgg aagcctacaa tcctgtcgcg cgcagcgcca cgatgggttc
     3001 gctgcgatcc cacaattcgc tgaatcgccg cgtgagcagc accgcctcgt gcccgtcgtg
     3061 cggatgggcc acgcaatcgg gcttggcggt gtcctgctga tacagatagc catgatgatc
     3121 cacgaccatg aacatttcgc ggatgccgag gtgctcctcg gcgacctgct gcatgtgaat
     3181 gaagtcgctc aaccgccggc acaatccgac caggcgatcg ttgtcgcgca aggtctgttc
     3241 cgtgtcttcg accacgatgc gagccagatt ctggcgatgc cgcgcggcga agcttgccag
     3301 cgcgcgggcg aagcgggaag tattgaaaaa cgcgccgtcc agccgtggcg cgaagatgac
     3361 gatctcgcgc gtggcatttt ccgccagcat ggccaggaaa tcgctgacct cggcgcggtc
     3421 acggatgatg cggcgctgtg ccgctgtctc ggtggctggt ggattcttgg ccgtttccat
     3481 atgcgcgatg aaaattctcc tgacggcgga aaaccggcgc catcaggata tatcagtagc
     3541 cctacttcgc ttcgctccgt tcgtcttgtc ataagaatag actacttcgc gtgcccggaa
     3601 gcggaggggc gtggatcaga ttttgatttt ggcgaggttt ctggcctgca tggccgcgtt
     3661 gccggtgtag gtgtcgggcg tcagtgccag caggcgtttt tttgcgttat cggggatgtc
     3721 gagcgtgcga ataaaggcct gcatggattc gcgggtaatg ccgccttttc cgcgcgtgag
     3781 gtccttgagt ttttcatagg cctgctcgac cccgtggcgc cgcatgacgg tctgtaccgg
     3841 ctcggccaga acctcccagt tctggtcgag gtcctggcgc aggcgctccg cgttgacccc
     3901 gagcttgccg atgcccttga ggcaggagtc gcaggccagc agcatgtagc cgaagccgac
     3961 accgaggttg cgcagcacgg tggagtcggt caggtcgcgc tggaagcgcg agaccggcag
     4021 tttctcggac aggtggtgga acagggcgtt ggccagcccc agattgcctt ccgaattttc
     4081 gaagtcgatc ggattcacct tgtgcggcat ggtggaagaa ccaacctcac ccttgatcac
     4141 cttctgcttg aagtagccga gtgagatgta accccagacg tcgcgattga aatcgatcag
     4201 gatggtattg aagcgcgccg ccgcatcgag caattcggcg atgtaatcgt gcggttcgat
     4261 ctgggtggta taaggattcc agtcgagccc cagtttctcg acaaaggctt cggcaaaggc
     4321 cggccagtca acctccggat aggctgcgag atgcgcgttg taattgccca ccgcgccgtt
     4381 gatcttgccc aggattggca cgtccgccat ctgtttgcgt gcgcgtttca ggcgatacgc
     4441 gaaattcgcc atttccttgc ccagcgtggt gggcgaggcc ggctggccgt gggtgcgcgc
     4501 cagcatcggc tgctcggcat ggcggttgac gaggtcggtc atggccgcga tcaacttgtc
     4561 catgcgcgca agcaatacgc cgtcccggct ttgtttcagc atcagggcat aggcaaggtt
     4621 gttgatgtct tccgaggtgc aggcaaagtg aatgaattcg gtgcagcggg cgatctcctt
     4681 gttgtcgcgc gttttctcct tcagaaaata ttcaatcgcc ttgacgtcgt gattggtggt
     4741 cgcctcgatt tccttgacgc gccgcgcatc ggcctcggaa aagtccgcga cgatggcgtt
     4801 caattgtttt tgcgtggcgg aggagaaggg cgccacttcc ttgatcttct tgtgctctcc
     4861 cagcgctatc agccagcgta tttccacgag cacccgatga cggatcaggc cgtactcgct
     4921 gaatagcggt cggagttctg cggttttggt ctggtagcgg ccgtcgagtg gcgagagggc
     4981 ggtgagcgaa ttcagcggca atacatccgg tttgttcggt ttgttcatgc gcgcctgcga
     5041 tggttagtgg tccgtgtggg cggacgcatt ttaccagaac aggccggaaa aagccgtgtc
     5101 agcgctgcgc tgaatgcatc tcgacagcgt gtgtcgcggg catggcgtcc tcaatgatcc
     5161 cgccgcccag acactcatcg ccatcgtaga acaccaccga ctgcccgggc gtgatggcgc
     5221 gctgtggatg atcaaacgtt acacgcaggc ctttgtcatg cggtgtgacg gtgcagtcct
     5281 gatccggctg gcgataacgg gttttggcgc gcagtttccg cggccaggga aagggttcag
     5341 cgccgatcca gtgcagctga ccggcgatca gtgcctcgct gaacagtgct ggatgctgtt
     5401 caccctgttc gacatagagg acattattcg ccacgtcctt gcccaccaca taccatgcct
     5461 cgccctcgcc gccgatgccc agcccatgac gctggccgat ggtgtagtac atcacgccat
     5521 catgttttcc tttgagcgcg ccggtgagcg tgcgcatctc gccgggctgt gcgggcaggt
     5581 agcgcgcgag aaaggatttg aaatcgcgct cgccgatgaa gcagatgccg gtcgaatcct
     5641 ggcgatcgta attcggcagc cccgcctcgc gcgccagccg acgcacctcg gttttggtca
     5701 tatcccctac gggaaacagc gcgtgcgcca gagcttcctg tcccagcaga tacagaaaat
     5761 aggattggtc cttgttgcca tcgcgcgcct tcagcaggcg aaagcgcccg gcgtgctggt
     5821 cggcgcgcgc gtaatggccg gtggcaatac ggtcggcgcc aagtgtgagc gcgtgatcga
     5881 gaaatgcccg gaacttgatc tccttgttgc acagcacgtc cgggttgggt gtgcggcccg
     5941 cccggtattc ggacagaaaa tagctgaaca cacgatccca gtattcagcg gagaagttga
     6001 cggtcttgag agggatgcca agatgatcac agaccctctg cgcgattttc agatcttccg
     6061 ccgcgccgca atggcccgcg gtgtcgtctt cctcccagtt cttcatgaac aggcccgtga
     6121 cttcgtggcc ctgacgcttc agaagcagcg ccgccacggc cgagtccacg ccaccggaaa
     6181 tgccgacgac tactttcatc gatctttata ctcagcgaga agttcgagcg ggtaacgtcg
     6241 gccggcgaga taatcgttga ggcagcgctc cacctgcggg ctgcggtgcc gtgcgcgctc
     6301 ggcgcggatt tcctccgggg tcagccacaa tgcacgcacg atatcatgat cgagcgcacg
     6361 gttttcttca tgacttgtca catggccgat gaaggcgaag cgcagataag tgatgtcttt
     6421 ggtgggatgt ggccagcggt aaacgccgag cagtgcctcc ggcgtgaaat gccatgcggt
     6481 ttcttccagt gtttcgcgca ccgccgcctg catcaggctt tcgccctcgt ccagatgacc
     6541 ggccggctgg ttgagcacga gcctgccgtc ggctttttcc tcgaccagca ggaatttccc
     6601 gtcacgctcg atgatcgccg ccaccacgac attcggtttc cagatcatgg gaaaacaatt
     6661 taacaggatt aacaggattt ttcaggattc acagggcgaa gcgaagtagt cgcctatgta
     6721 cttaacgtac atcggcgccg ctgagcgccc gcacacggat gtgcgggccg ggataaaacg
     6781 aagcctctgt gtgacgctag ggacggcatg cctgccatcc gtggcgtgac cacaacgact
     6841 tcgaactacc ccggccccgc catccttggc ggggcgctcg ccgggacgaa gacacgttaa
     6901 gtgtcttcgt ctctttccgg ctcgccttgt taatcctgtc tattcatttt gttttccctg
     6961 caaaatgatc atgaccgctc tgttgcggtt gatagggtct ttccgattca tcaaagatac
     7021 gcaaacccgg ttctgcttcg atcgtgccga tatggtgaaa tccacaggca ggatgcagct
     7081 tttccagcgc agcgatattt ttctccggca cggtaaagca cagctcgtaa tcatcgccgt
     7141 tcgccagcac catgtcccag ccggtttcgc ggatatgcga ccggcaaacc ggtgagacgg
     7201 gtattttatc cagactgatg cgcgcgccga cctggctcat ttccaggata tggcccagat
     7261 ccgccagcag cccatcggaa atatcgatgg cactgtgggc aatgtctcgc agcatcatgc
     7321 cctcgcgcac gcgcggcgtg ggacggtcca tccggtcggc aaccgctgcc agatcgcgtt
     7381 ccggcaggcg gataccgcca agacggtgac gcagcgccaa cgcagcatcg cccagcgtcc
     7441 ccgtcacata gatgcggtca cccgccttcg cgcccgagcg tcgcagcgcc tgttcccttg
     7501 gtacgaaacc atgcgcctcg atggcgatgg tgagcggccc gcgcgaggta tcgccgccaa
     7561 cgagttgcac cttgtgttca cgcgcgagtt cgaacatccc gcggcagaac agctgaagcc
     7621 agttggcatc ggccttgggc agcgccagat tcagcatgaa ccaggccggc tccgcgccca
     7681 tggatgcgag gtcgctgaga ttcacggcca gcgccttgtg accgagagaa aacggatcga
     7741 catcgggaag aaaatgcacg ccggaaacca gcagatcgga agtcaccgcc agttgctggc
     7801 cggcgggcgg ttgcaacagc gcggcgtcgt caccgatgcc ggcagcgaca tcggggcgcg
     7861 cgacatcctg gcgcgcaaaa aacgtgcgaa tcagctcgaa ttcgttcacg gccgaattca
     7921 ggccggagaa ctctcggcct tgatttcgcg cgaacgcaga cccgtggcga tcttgtcgag
     7981 aatggcgttg acgtacttgt ggccgtgttc ggcgccgaag gtcttggcca gctccacggc
     8041 ctcgttcagg atgaccttgt agggaatctc ggggtgaaac tcaaactcat aggtgccgat
     8101 gcgcaggcac gcaagctcga cagggtcgac gtcgttcagc ttgcggtcca gatgcgggga
     8161 aatgtgatcg tccagctcgt gcagatgcag cgggatctcg cgcaccagat gatggaagta
     8221 atcgagatcg acaccgttca tctcgtgatc gaggataaaa tgagcctcga tctcacgcgg
     8281 cgcctgctcg gtgagttgcc actgatacag ggcctgcacg gcggcgcgac gtgccttgtg
     8341 acgactgccc ttgaccatag accctccgtc agacgcgcag ttttttgagc agattcacca
     8401 tctcgatggc ggtaagggcg gcgtccacgc ccttgttacc gctcttggtg ccggcgcgct
     8461 cgatggcctg ttcgatagtg tccaccgtca gcacgccgaa agcgaccggg aggtcgaatt
     8521 gcatgccgac ttgcgagacg cccttggcgc attcgccggc gacgtattca aaatgcggcg
     8581 tctcgccgcg gatcaccgcg ccgagcgcaa tcagtgcatc gtattttccg ctggccgcca
     8641 tggttttcag ggccagcggc atttcgaaag cgcccggtac gcgaacgact tctatgttag
     8701 cagcttcggc cccatggcgc accagcgtgt cgatcgcgcc ttccagcagg cgctcgccga
     8761 tgaagctgtt gaaacgtccg agagcaattc caaaacgcgc gctgcgcgcg atcaggtcgc
     8821 catcaatgct ttttacgtca ttcatccaga tacctttttc tctttgttgt gtttgcgatg
     8881 agtacttgag agttgcgctg ggtcgttttc aatgtattcc acgatctcca gtccgaagcc
     8941 cgacaggccg ggcgccttca tggcatggcc cagcacgcgc attttgctca cgccgagatc
     9001 ggccaggatc tggctgccca gaccgtaggt gcgcatttcc tggtggccgc gcttgcctgc
     9061 gcccgattct aatgctttgc cctgcccctg aaaatgccgg atacgttgca ccagatcggc
     9121 gggcttctct tcctgttgca gaatcaccac cacgccagcg ccttcggcgg ccacgtgcgc
     9181 gagcgcctcg tgcaacgacc aggaaccggc gcgatgcgcc gtggtgacga gatcgagcag
     9241 gctttcctgc atgtgcacgc gcaccagcgt cggccggtcg cggcggatat cgcccctgac
     9301 cagagctagg tgcacggaat cgccgatgtc atcgtgataa gccaacacgc ggaagtcgcc
     9361 gaattccgtc ggcatcgtgc attcgccaac gcgttgaatg gtggattcat gttcggtgcg
     9421 gtagcggatg agatcggcga tggtgccgat cttgagccca tgttgttgag cgaatttttc
     9481 cagctccggc agacgcgcca tggtgccgtc ttcgttcatg atctcgcaga tgacgctggc
     9541 gggttcgaac ccggcgagac gcgcgaggtc cacgccggcc tcggtgtgtc cggcgcgcgc
     9601 cagcacgccg ccgcgctgcg ccatcaacgg gaacacatgg ccgggttgca ccagatcgct
     9661 cggtttggcg cccctcttca ccgcgacctg gatggtgcga gcgcgatcag cggcggaaat
     9721 gccggtggtc acgccttccg cggcctcgat ggtgacggtg aaattggtcg agcgccggtg
     9781 gtgcgtgtcg ctcaccatga gcggcagctg caactggcgg caacgttcct gggtcagcgt
     9841 gaggcagatc aggccgcggc cgtagcgcgc catgaagttg atgtcggagg cagcggcgtg
     9901 cgcgccggcc atgaccaggt cgccttcgtt ctcgcggtcc tcatcgtcca tcaggacgac
     9961 catgcggccg cgcttgatct cgtcaatgat ctcggtaatc gggctgattg gcataaaacc
    10021 acgggaaaag accgaatcgg gcctgcatta tcacctattt tcgcatcgga tgccctgatt
    10081 ccggccgggc cggtctgctc aacaatggcc gccgtttatc ggggtttatc cggcttttat
    10141 cctggctttc ctcgcccatt ccggttccgt ggccatactc cttatataga agcgggtact
    10201 gccaggggtg gtggcgtccg tccgtgaacc atttaccaac tgggtgccga ttatgtctgc
    10261 caaagccaac aaggccgaca aactgcaaag cacggtcatt atttccggtc gttttagcga
    10321 acgccgggcg caacacgcaa cccaacccgc tgaagaatcg tccgcggagc cgctcgaacc
    10381 cggcagcatg gtcggccgct acctgacgct gagccggatc ggcgtgggcg gcatgggtat
    10441 cgtgtaccgc gcgcacgata ccgaactcaa tcgtacggtg gcactcaagg tgctgccgcc
    10501 caagttcagt caataccccg agtacctgaa acggtttcac accgaggccc aggcccaggc
    10561 acggttgaac agccctcacg tcgtcacatt gtatgagctg atggaccatc ccgtgggcca
    10621 ggtgttggtg ctcgaatacg tcgaaggcga aacgctcgaa agccgcctgc gccactacgg
    10681 ccccctgtcc gtacccgagg ccgtg
//
LOCUS       DBNGZE010000431         1860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1450234, whole genome shotgun sequence.
ACCESSION   DBNGZE010000431 DBNGZE010000000
VERSION     DBNGZE010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1450234"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="AC16FV_11145"
     CDS             complement(<1..131)
                     /locus_tag="AC16FV_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR2850644.1"
                     /translation="MIRAIQADITTLKIDAIVNAANESLLGGGGVDGAIHRAAGPQL"
     gene            complement(148..381)
                     /locus_tag="AC16FV_11150"
     CDS             complement(148..381)
                     /locus_tag="AC16FV_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850645.1"
                     /translation="MMFIAVPARAADEAQSGRAERPKREAVKLSPSVYRSAPRPDESG
                     KVKRVAPPRAESPLQNVPALMDPPPGGESPAKR"
     gene            complement(653..1051)
                     /locus_tag="AC16FV_11155"
     CDS             complement(653..1051)
                     /locus_tag="AC16FV_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR2850646.1"
                     /translation="MTPIKLYLLRAVYDWAVDNNFTPQVLVDATAAGVMVPVNYIQDG
                     RIVLNIHPRAVGHFEFAAETLRFSARFGGQSLSVEVPYTAVLAIYARENNQGITFPEL
                     PAGDKPGTGPEDNPPGDKPPGKGPSLRVVK"
     gene            complement(1104..1799)
                     /locus_tag="AC16FV_11160"
     CDS             complement(1104..1799)
                     /locus_tag="AC16FV_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR2850647.1"
                     /translation="MRFTASGGKPPGSDMIDKNAMTIPAARKPIMTLYSGTSDPWSHR
                     TRIVLFEKDIECQIVDVDVNKKPRELGELNPYNQVPTMVDRDLVLYESNIINEYLDER
                     LPHPPLMPVDPVSRARARLMLVRFDRDWYGLIHDIIGGDKKAATKARHTLRDGLTVIS
                     PIFKEQTFALGEEFSLVDCSLAPLLWRLDHWDIELPRQAKPILDYANKLFQRKSFKLS
                     LTEAEKAMRGPSR"
BASE COUNT          347 a          537 c          586 g          390 t
ORIGIN      
        1 ttcagttgcg gtcccgcggc acggtggatg gcaccgtcca cacccccacc gccgagcagg
       61 gattcattgg cggcgttgac gatggcgtcg atcttgagcg tcgtgatgtc ggcctgaatc
      121 gcacgaatca tcgggcaact gaggacgtca gcgtttggcg gggctttcgc cgccgggcgg
      181 cggatccata agcgccggca cattctgcaa cggcgactcg gcgcgcggcg gcgccacgcg
      241 ttttactttc ccgctttcgt ccggacgcgg cgccgaccgg tacacgctgg gcgacagctt
      301 caccgcttcg cgctttgggc gctcggcgcg tccggactgt gcttcatcgg cggcgcgcgc
      361 tggaacagca ataaacatca tggtgacaat taggaggaac tttaatgtgc gcaaaaccat
      421 ctcccgaaaa acaatagaca ggattaacag gatttttatg gatttacagg attaaagatt
      481 tggggaacta aaatcctgtt aacttgtgaa tcctatctga tgttgtcttt cttggcgtcc
      541 ttggcgtctt ggcggttcaa aactattaat aaagctttct ttaacataac ccaaaaggcg
      601 ggataccgga cataatcgcc gcaccggcgg cgcgaagcgg gcgatgtgcg cgctatttca
      661 cgacgcgcag gctcggtccc ttgcccggtg gtttgtcgcc cggcgggttg tcctccggtc
      721 cggtacccgg cttgtcgccg gccggcaatt cgggaaacgt gatgccctga ttgttttcgc
      781 gcgcgtagat tgcgagcacg gcggtgtacg gtacttccac ggaaaggctc tgaccgccga
      841 aacgcgccga gaaccgcagg gtttcggcgg caaattcgaa atgacccacc gcgcgcggat
      901 ggatgttcaa cacgatgcgg ccatcctgga tgtaattcac gggaaccatc acgcccgcgg
      961 cggtggcatc gaccagcacc tgcggcgtga aattattgtc caccgcccag tcgtagacgg
     1021 cgcggagaag atagagcttg atcggggtca tgcgccgcgc ggcgtttgtg cacgcgggag
     1081 aatcgggcgc ggggtccgcc gcttcaccga gaggggccgc gcattgcctt ttccgcctcg
     1141 gtcagcgaca gcttgaagga cttgcgctga aacagcttgt tggcgtaatc gagaatgggc
     1201 ttggcctggc gcggcagttc gatgtcccaa tggtccagcc gccacagcag cggcgcgagc
     1261 gagcaatcga caagcgagaa ttcctcgccc agcgcgaaag tctgttcctt gaagatcggc
     1321 gagatcaccg ttaagccgtc gcgcagcgtg tgacgcgcct tggtggcggc ctttttgtcg
     1381 ccaccgatga tgtcatggat caggccgtac caatcgcggt caaaacgcac cagcatgagc
     1441 cgggcgcggg cgcgcgacac cgggtccacc ggcatgagcg gcggatgcgg cagacgctcg
     1501 tcgaggtatt cgttgatgat gttggattcg tacagcacca ggtcgcggtc taccatggtc
     1561 ggcacctggt tgtacggatt gagctcgccc agctcgcgcg gcttcttgtt gacgtccacg
     1621 tccacgatct ggcactcgat gtccttctcg aacaggacga tgcgcgtgcg gtggctccag
     1681 ggatcggagg tgcccgagta gagcgtcatg atcggcttgc gcgccgcggg tatggtcatc
     1741 gcgtttttgt cgatcatgtc gctcccgggc ggcttgccgc cgctggccgt gaatcgcaca
     1801 gcttactcaa gttactcagt ggatgtcctt ccagtattcc ttcttgagca gataggcaaa
//
LOCUS       DBNGZE010000432        19623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1727556, whole genome shotgun sequence.
ACCESSION   DBNGZE010000432 DBNGZE010000000
VERSION     DBNGZE010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 19623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19623
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1727556"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16FV_11165"
     CDS             complement(<1..202)
                     /locus_tag="AC16FV_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR2850648.1"
                     /translation="MSEKLQKVLARAGLGSRRQIERWIEEGRISIDGHPAALGARVTP
                     DQALRVDGRIVPAHAFQTKPRIL"
     gene            complement(195..884)
                     /gene="scpB"
                     /locus_tag="AC16FV_11170"
     CDS             complement(195..884)
                     /gene="scpB"
                     /locus_tag="AC16FV_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR2850649.1"
                     /translation="MSEPNTELKNIVEAALLVAGQPLTIDKLLTMFPAESPPTREEIR
                     AVLEMLEEEYAERVVELKQIDRAWRFQTRDKYAPWITRLAEERPVRYSRALLETLAII
                     AYRQPVTRGDIENIRGVSVSTDIIKTLQGREWIRQVGVRNVPGRPALYGTTREFLEHF
                     NLKNLEELPPLSALRDLDVISNELNMRLDLEQGAVPAAEGAAAADAESHGVLEQEPEP
                     IMSEARTAERE"
     gene            complement(884..1675)
                     /locus_tag="AC16FV_11175"
     CDS             complement(884..1675)
                     /locus_tag="AC16FV_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR2850650.1"
                     /translation="MSTPTDTEAPVAVVRGETLTRLPEDLYIPPDALEVFLETFQGPL
                     DLLLYLIRKQNLDILDIPIAEVTRQYMGYVELMQTVKLELAAEYLVMAAMLAEIKSRM
                     LLPRPAGEDGEEADPRAELVRRLQEYERYRTAAESLDQLPRVGREVHLVVIENDQPPV
                     KRIEPNVSLFELMDAFREVLVRAELYRHHHVQLEPLSVRERMTRILAKINSEAFVSFE
                     DMFDLNEGRRGLVVTFMAILELLKESLVVVVQQEPFAPIHLKAAV"
     gene            complement(1675..2895)
                     /locus_tag="AC16FV_11180"
     CDS             complement(1675..2895)
                     /locus_tag="AC16FV_11180"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR2850651.1"
                     /translation="MSAVLPGQPMRVLSGMRPTGRLHLGHYHGVLKNWVKLQAEYDCF
                     FFVADWHALTTHYEDPHIIEESVWDMVVDWLAAGVDPNNATLFIQSRIPAHAELNVLL
                     SMITPLSWLERVPSYKDQQLQLKERDLSTYGFLGYPLLQSADILVYRAGLVPVGEDQV
                     AHVELTREVARRFNHLYGREPGYEGRAEEAIKKMGKKNAKLYRDLRTAYMEQGDREAL
                     ETARALLERQKNITLGDKERLFGFLEGGGKIILPEPQAMLTQAPKMPGLDGRKMSKSY
                     DNVISLRDPPAEVEKKLRTMPTDPARVHRDDPGNPDKCPVWDFHLIYSDDERKDWVQK
                     GCRSAKIGCLECKQTIIDAVLAELKPMQERAVEFSRDPLLVKNIISDGCKRAQDAAEA
                     TLVEVRAAIGLGYT"
     gene            complement(2892..3545)
                     /locus_tag="AC16FV_11185"
     CDS             complement(2892..3545)
                     /locus_tag="AC16FV_11185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR2850652.1"
                     /translation="MPNLSLPQFFAVAALPLLFAITVHEVAHGWVAKLYGDPTAQRLG
                     RLTLNPLKHIDPIGTVLVPALLLALGGFVFGWAKPVPVTWENLRHPKRDMAIVAAAGP
                     GANLLMALFWALIAKLGSVLGASSWAGLPMQYMGFFGIEINAVFMILNLIPLPPLDGG
                     RVAVGLLPGPWAWQLARIEPFGFFILLALLATGVLGMLIGPPIHGFERIVTSIVGLQ"
     gene            complement(3624..4247)
                     /locus_tag="AC16FV_11190"
     CDS             complement(3624..4247)
                     /locus_tag="AC16FV_11190"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR2850653.1"
                     /translation="MAQYFQIHPVNPQSRLIKRAVEIVRAGGVIAYPTDSSYALGCHI
                     GDKQAMERIRRIRRVDDTHNFTLVCRDLSEVAQYARVGNADYRLLRANTPGPYTFILP
                     ATREVPRRLQHPKRKTIGLRIPDHVIAQSLLTELNEPLMSSTLILPGDDLPLSDPEEI
                     RTHLEHELDLVIDGGFCGLDPTTVIELEDGAVTLVRKGKGDAARFAA"
     gene            complement(4321..5235)
                     /locus_tag="AC16FV_11195"
     CDS             complement(4321..5235)
                     /locus_tag="AC16FV_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR2850654.1"
                     /translation="MSSSNSISEPVPSRGYDMHTHSLRSDGTLTPSELVERAHAAGVR
                     VLALTDHDVTDGIAEAQSAADRLGIKLIAGVEISVTWQNQTLHIVGLNICPDQPELQQ
                     GLSGLREFRQWRAEEIGRRLQKKRIEGAYEGAARLAKGTIISRMHFARFLVDEGYVRG
                     PQQAFRQFLSRGKPGHVPGRWATLAEAVQWIVSAGGEAVVAHPARYKLSAGKLRQLLG
                     EFRECGGTAIEVISGSHTPDANRHFAGIATEHGFLASAGSDYHGPEKTWMDLGRLPAL
                     PDGCIPVWQDWNMNMKPPINTDEHRLVN"
     gene            5251..6033
                     /locus_tag="AC16FV_11200"
     CDS             5251..6033
                     /locus_tag="AC16FV_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850655.1"
                     /translation="MNNQLFRDGTLSPMAITSLPPTLAEAALRFKHAAAEINDTYEAR
                     RPDKTNQVTPGQLTEAIEQFLTIIERIDREEGKVGPIAKDDATQLGDYGMTLIADLSA
                     WAGQLNLPVPRQDLEIVTLAAADWIVRHDGQIRTLEPVVNALANFANRLHEPTSLEPL
                     ITLMGRVAQAAANLIKQDLEKNNPGRPWRVLHLNRGIVATRTHNPELMEKVFDDLVRY
                     LPEDAAAFFAEGMQQMEELDYPQHVRQVMNRYFDRWTRPRMH"
     gene            6135..6728
                     /locus_tag="AC16FV_11205"
     CDS             6135..6728
                     /locus_tag="AC16FV_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIR2850656.1"
                     /translation="MKFLYDLFPLLLFFAAFKLYDIYAATAVAIAASFVQAGHFWLKH
                     RRFETMHVVTLGVIVVFGGMTLLLHDDTFIKWKPTLVYWILSALVLASQWIGNKTVIE
                     RMMSSQIVLPATVWKRLNLSWGIFFAALGAVNIYVAFYYGLDLDAATRQSIWVNFKVF
                     GLLGITLVFVIAQAFFMAKHVHDKPETDASSSNRTAD"
     gene            6728..7027
                     /locus_tag="AC16FV_11210"
     CDS             6728..7027
                     /locus_tag="AC16FV_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR2850657.1"
                     /translation="MWYVINATDNEGSLERRLSVRARHLARLQELQNEGRLLLAGPYP
                     AVDGNDPGPAGFSGSLIVAEFDSLEDAQSWANADPYVEARVYASISVKPFRKVLP"
     gene            7027..7293
                     /locus_tag="AC16FV_11215"
     CDS             7027..7293
                     /locus_tag="AC16FV_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR2850658.1"
                     /translation="MSRATLIESRLREAFNPSKIEVRDDSHRHAGHEGAKSGGGHFTV
                     TIVAPQFQGRNAVQRHQMIYRALGDMMKKEIHALSIQALAPDEL"
     gene            7338..8198
                     /locus_tag="AC16FV_11220"
     CDS             7338..8198
                     /locus_tag="AC16FV_11220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2850659.1"
                     /translation="MSSLRTTLIVLLAAVGLFAAGCDKKPAGEAGKEAAKDADKVFAT
                     VNGEKIMGADYQNYLQLRQQQVGPLPDKDKEKKIVLDEMIEKMLLAQHAVSTKLDQDP
                     EVSGLMKRVREEILAQAVKRKLLRDNPITDDDVKKRFEQEVEATHKTEYKVRHILVKD
                     EAEAKDILAQLGKGAKFDKLAKDKSMDTQSGKNGGELGWVNQGMVVPEFFNAVMAMKK
                     GAVSTEPVKSDFGWHIIKVEDTRPLKIPTFEQFMSDQRARANIYRKMQDDRIGNLVKD
                     LKGKAKITVD"
     gene            8262..9269
                     /locus_tag="AC16FV_11225"
     CDS             8262..9269
                     /locus_tag="AC16FV_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HIR2850660.1"
                     /translation="MRHLFKITGTIAAMLYVGLHSGLLAAQTDLETRQVVLSQNIKIG
                     DRIGRIRFLGMLQLPDVTRNGLRLSQLSGLAWDDDDGVLYALSDKGNLFHLKPQFDGD
                     FLMGLYLLRAVPLQDPGSNKSFMRPRTDSEGLDILNGRNGRKGDAELVISFERVPRIG
                     RYRPDGSALGDYPLPEPLSGAKNFTGANRMLESVCVDSSLGVITAPEEPLKNEQSGST
                     RIFSLSSGSWRYPVASGDRLTAMECLGQGEVIFLQQTYLHAFGQITVSLKRVRLASNP
                     SSEPLKPETLVTLDSQQGFEIDNFEGLTRHKGRRFFMVSDNNDLFVQRTLLMYFELLD
                     E"
     gene            complement(9296..9748)
                     /locus_tag="AC16FV_11230"
     CDS             complement(9296..9748)
                     /locus_tag="AC16FV_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR2850661.1"
                     /translation="MLRLWRNFLVIVFLIHLCGISTVAESVTMPKRLSPCPDSPNCVS
                     SDATDASHRVEPFEIIFPSDVAWSLAREAVNNLPRTKITQATDNYLRAECTSAVFGFV
                     DDLELELRMDKGVIAVRSAARTGYWDFGVNRRRAESLREALRSRGVVK"
     gene            complement(9803..10660)
                     /locus_tag="AC16FV_11235"
     CDS             complement(9803..10660)
                     /locus_tag="AC16FV_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR2850662.1"
                     /translation="MTAAALALAAAVLMHVAWNLLARASHPDSRFLWWALIGYLVVLG
                     PWSLYALITQAHWSLGLAGLLVISALANSLYFLVLGAAYRHAPVALVYPIARSSPLLI
                     ALWSSLLFGEAIAASGWLGILISVAGLLLLAASARHGDSRRAVMWAVIAAFATSMYSL
                     SNKFAVSSLPTYGSQLGLVSVDLMASFIALSLEQRVRLGRWRPSAMPPVSRWLPAGLC
                     IGNAYGLVIYALQYLSAAYAVAFTNAGIVIAGLLSIFAFGEREHARTRLIAIAVITGG
                     LAVLVLSAR"
     gene            complement(10753..11286)
                     /locus_tag="AC16FV_11240"
     CDS             complement(10753..11286)
                     /locus_tag="AC16FV_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2850663.1"
                     /translation="MRYRRAGVPGASYFFTVNLADRERTLLVDLVDVLRTVIREAQSR
                     HPFRIDAMVVLPDHLHAIWTLPKNDKDFSTRWMLIKSGFSRRLSAGERRNRSRVAKGE
                     RGIWQRRFWEHLIRDEKDYERHVDYIHYNPVKHGYVARPVDWPHSSIHRFITEGIVSP
                     DWAVGNQDENSNKYGER"
     gene            complement(11986..13998)
                     /gene="ligA"
                     /locus_tag="AC16FV_11245"
     CDS             complement(11986..13998)
                     /gene="ligA"
                     /locus_tag="AC16FV_11245"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR2850664.1"
                     /translation="MSGADKLRKRAEELRKEINYHNYRYYVLDNPVISDAQYDKLLRE
                     LQQIESDHPKLITPDSPTQRIGAAPLSEFGEVRHKIPMTSMDNAFDDNEAREWDQRVR
                     KGLETEAAVDYTAEPKFDGTSVSLRYESGVLMQAGTRGDGTTGEDVTANVRTIKTVPL
                     HLQGKGWPKVVEVRGEVVIPKKDFEHLNAEQLKQGGKVFANPRNAAAGSLRQLDPRVT
                     AARPLSFFPWGLGETSEPVARRYSEVSKHLKDWGFRVTEFFRVVHGADEYLEYYREIL
                     ARRDKLPFEIDGVVYKVDDLRSRDQLGFTARAPRWAIAHKLPAQEETTVVEDILASVG
                     RTGVITPVAVLKPVQVSGVTVTHATLHNQDEVERKDVRIGDTVIVRRAGDVIPEVVGV
                     IKDKRPRGAKAWRMPKKCPVCDSEVIREEEEAAHRCMGGLVCSAQRMGAILHFASRHA
                     MDIEGLGDKLVQQLVDKGLVKTVADIYRLKKDELADLERMADKSAQNLLDQIEKSKTT
                     TPARFLHALGIPQVGEATAQLLADHFESLDDILDAKRETLEQIHGIGPNMAEDISSFF
                     HEKHNREVIRALVKAGIHWPKPVRVKKSSALVGKTFVLTGGLASMSRDEAKRRLQELG
                     AKVSGSVSKKTDYVIVGEDPGSKADKARELGVTMLNEKEFLKLISK"
     gene            complement(13995..15200)
                     /locus_tag="AC16FV_11250"
     CDS             complement(13995..15200)
                     /locus_tag="AC16FV_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HIR2850665.1"
                     /translation="MNLQYALLFIGIVIVAVVALTAYDMSRQRRPRPRPEVHLGGFPD
                     DHAPVRPSLLDATREEGSDKVLRSDIEIAPREKPRDEILRKEIRVLEEMATMPLNLKP
                     GIQRRLRPRAEPDRQYLPDAKIDFVIDLPGPGPVMRDVALSVFKQNEYNLNKARHLYG
                     RHHGTERWSDLQLDPGSTEYDDIKLAIQLTDSRGPIDESELNAFMQIGLKLADALARP
                     TKLPETFEAGLARARELQEFCDTHDVIAGIHVVPNTEAAFPGRAIEMAARHAGLELGA
                     MNIFHKKNEVAPGSRYLFSLANLDESSGFDPAAWDRFETGGLTLFMSVPCVFQPGVVF
                     DRMATTAREIADTLGGKLQDQNRRPLSDKGIAVIHHQIEEIEEKMRAFGIPPGSETAL
                     KLFNEAATL"
     gene            complement(15200..18706)
                     /gene="smc"
                     /locus_tag="AC16FV_11255"
     CDS             complement(15200..18706)
                     /gene="smc"
                     /locus_tag="AC16FV_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR2850666.1"
                     /translation="MRLKTIKLSGFKSFVEPITVPITGNLIGIVGPNGCGKSNIIDAV
                     RWVMGEMSAKNLRGDSMADVIFNGSNARKPVAMASVELIFDNSEGRAGGEYAKYAEIS
                     IRREAGRDGQSDYYLNKTKCRRKDITDIFLGTGLGPRAYSIIEQGMVTRIIEAKPEDL
                     RGFLEEAAGVSRYKERRRETESRIKHTRENLTRVEDIRKELETQLSKLHRQSRAAARY
                     KELKQEERLTRAQLFVLRWVELDARVQSQDTLLAQQQTALDAVLAAQRGIEAEIEKIR
                     AQQTETVDQFNAVQAEFYSVGAEISRLEQAIQYARETRENQEREQEQINRAWGEASAH
                     LQSDVTRMEELKRRLEELTPQLEELARARDAAAESRAQAERAMQDWQNEWESFSELAA
                     EPARVREVQTARMRQLESHIEQLRQRQSRLEQEAAAIGSELEKEPANALATQVAELDN
                     SCEAQERSIGEIETRLREARDRRDELDDELAEMRGQKQSGEARLASLRELQAAAQGES
                     DAALGEWLRGQGLDQAPRLARLLKVESGWEKAVERVLGVNLGAVCVPRVEQIASAASG
                     FDRSQVSFVELGTSSTHQPSTRAALIDKVHGDIDLAPLLEGVYITELLEQALVMRAAL
                     STRESIVTRDGAWVGRNWLSLGSEKGARAGWLLREREIETLQADLSGWQGKLGNLQAE
                     IAELDTQLQNLEDERDELTRDLSENNRERARLREQLGHKQARLSQLESRRAQIEREQA
                     EVNEQLGRDQAEISAAGDLLRQAEATGGEHEQRRAQLQQNRQSLLEAVEQTRAQESAA
                     RDSAHRLEIERETQQTAFEATRASHARLEGQLQQLIARREQLSLALSSEQDPTPGYRQ
                     QLDEFLQKRLGIEERLNAARQAVTDLETQLREQEQARAREEHKTGEIREQHESERVAR
                     QELAVRRDTVADQLRESGFEVEPVRAELPPEANETEWAARLEQVTARIERLGPINLVA
                     IEEFEEANTRKTYLDKQYEDLSQALATLEEAIRKIDRETRTRFKETFDQVNEYFQGFF
                     PQLFGGGSAHLEMTDNDLLETGVSVMARPPGKRNSTIHLLSGGEKALTAVALVFSIFQ
                     LNPAPFCLLDEVDAPLDDANVIRYCDTLKNLSGKTQLVYITHNKISMEMADILIGVTM
                     SEPGVSRLVAVDVDQAMEMVAQ"
     gene            18785..19174
                     /gene="queF"
                     /locus_tag="AC16FV_11260"
                     /pseudo
     CDS             18785..19174
                     /gene="queF"
                     /locus_tag="AC16FV_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
BASE COUNT         3881 a         6116 c         5710 g         3916 t
ORIGIN      
        1 ggaggatgcg tggctttgtc tggaacgcat gggccggaac gatgcgtccg tctacgcgca
       61 aggcctgatc gggagtgaca cgcgccccca acgcagcggg atggccgtct atgctgatgc
      121 gaccttcttc gatccagcgt tcgatctgac gtcgcgaccc caggccggca cgggccaaaa
      181 ccttctggag tttttcactc acgctcggcc gtgcgggcct cggacatgat cggttccggc
      241 tcctgctcca gaacgccgtg ggattcggca tcagccgcgg cggcgccttc agcagcgggc
      301 accgcgcctt gctccagatc cagacgcatg tttagttcgt tactgatgac atccagatcg
      361 cgcagcgccg aaagcggcgg caactcttcc aggttcttga ggttgaaatg ttcgaggaac
      421 tcacgcgtgg taccgtaaag cgccgggcgc cccggaacat tgcgcacgcc cacctggcgt
      481 atccattcgc gcccctgcag ggtcttgata atgtcggtgc tcacggaaac accgcggata
      541 ttctcgatat cgccgcgcgt gaccggctgg cgataggcaa tgatggccag agtttcgagc
      601 agtgcacgcg agtagcgcac cgggcgttcc tcggccagac gcgtgatcca gggtgcatat
      661 ttgtcgcgcg tctggaagcg ccaggcgcgg tcgatctgct tcaattccac cacgcgctcg
      721 gcgtactcct cttccagcat ttccagcacg gcgcggattt cctcgcgcgt gggcggcgat
      781 tcggcgggga acatggttag cagcttgtcg atggtcaacg gctgaccggc gaccagcagc
      841 gccgcctcga cgatgttctt cagttcggtg ttgggttcgc tcatcagacc gccgccttga
      901 gatggatggg cgcgaagggt tcctgctgga ccacgacgac cagcgattcc ttcagcaact
      961 caaggatcgc catgaaggtc accaccagac cgcggcggcc ctcgttgagg tcgaacatgt
     1021 cctcgaaact cacaaacgcc tcgctgttga tcttcgccag aatccgggtc atgcgttcgc
     1081 gcaccgacaa tggctccaat tgcacatgat gatgccggta aagctcggcg cgtacaagca
     1141 cctccctgaa ggcatccatc agctcgaaca ggctgacgtt aggttcgatg cgcttgaccg
     1201 gtggctggtc attttcgatg acgaccaggt gcacctcgcg gccgacgcgc ggcagctgat
     1261 ccagcgactc ggcggcggtg cggtaacgtt cgtattcctg caggcgccgc accagctcgg
     1321 cgcgcgggtc cgcttcctcg ccgtcttcgc cggccgggcg cgggaggagc atgcgggatt
     1381 ttatttccgc cagcatcgcg gccatgacca ggtactccgc ggcgagttcg agcttgacgg
     1441 tctgcatcag ctccacatag cccatgtact ggcgcgtgac ttcggcgatg gggatatcga
     1501 ggatatcgag gttctgtttg cggatcagat aaagcagcag atcgagcgga ccctggaagg
     1561 tttcgagaaa aacttccaac gcatccggcg ggatgtataa atcctccggc agccgggtga
     1621 gtgtctcgcc gcgaacgacg gccacgggcg cttcggtgtc ggtcggcgtg ctcatcaggt
     1681 atagcccagt ccgatagcgg cgcgcacttc caccagcgtc gcttccgccg catcctgcgc
     1741 gcgcttgcaa ccgtcggaaa taatattctt gaccagcaag ggatcgcggg aaaattccac
     1801 ggcgcgctcc tgcatcggtt tcaattccgc cagcacggcg tcaatgatgg tctgtttgca
     1861 ttcaaggcag cctattttcg ccgaacggca gcccttttgc acccagtcct tgcgctcatc
     1921 gtccgaatag atgagatgga aatcccacac cgggcatttg tccgggttac ccgggtcatc
     1981 gcgatgcacg cgcgccgggt cggtgggcat ggtgcggagt tttttctcca cctcggccgg
     2041 cgggtcgcgc aggctgatca cgttatcgta ggacttggac atcttgcgtc cgtcgaggcc
     2101 cggcatcttg ggcgcctgcg tcagcatcgc ctgcggctcg ggcagaatga tcttgccccc
     2161 tccctccaga aaaccgaaca gcctttcctt gtcgccaagg gtgatgttct tctgccgttc
     2221 gaggagcgcg cgtgccgtct ccagcgcctc gcgatcgccc tgctccatat aggccgtgcg
     2281 caggtcgcga tagagtttgg cgtttttctt gcccatcttc ttgatcgcct cttcggcccg
     2341 gccctcatag cccggctcac gcccataaag atggttgaag cggcgcgcca cttcgcgcgt
     2401 caactccacg tgcgccacct ggtcctcgcc caccggcacc agaccggcgc gatacaccaa
     2461 aatatcggcg ctttgcagca gtgggtagcc gaggaagccg taggtcgaaa ggtcacgctc
     2521 cttgagctgg agctgctggt ccttgtagga cggcacccgc tccagccagg acagcggggt
     2581 gatcatggac agcagcacgt tcagttcggc atgcgccggg atgcgcgact ggataaacag
     2641 ggtggcgttg ttcggatcga ccccggcggc gagccagtcc accaccatgt cccacacgct
     2701 ttcttcgatg atatgggggt cttcgtaatg ggtagtcagg gcgtgccagt cggccacgaa
     2761 gaagaaacag tcgtattcgg cctggagctt gacccagttc ttgagcaccc catggtaatg
     2821 cccgaggtgc agtcggccgg tggggcgcat gccagacaag acccgcatag gctggccagg
     2881 caaaaccgcg ctcattgaag ccccacaatc gaggtcacaa tgcgctcgaa cccgtgaata
     2941 ggcgggccaa ttaacatgcc caagacaccg gtcgcgagta gcgctaacaa aataaagaaa
     3001 ccgaaaggct caatgcgcgc caactgccaa gcccaaggtc ccggcaaaag cccgacggcg
     3061 accctgccac catcaagcgg aggtagagga atcagattaa gaatcatgaa tacagcattg
     3121 atttctatac caaaaaatcc catatattgc atcggaagcc cggcccaaga gctggccccc
     3181 aacacactgc caagcttggc aatcagtgcc cagaaaagcg ccatcagcaa atttgctccc
     3241 ggacctgcgg ccgccacaat ggccatgtca cgcttggggt gacgcagatt ttcccaagtc
     3301 accggcacgg gcttggccca gccgaagaca aagccaccca gagcgagcag caatgccggt
     3361 accaaaacgg tgccaatggg gtcgatatgt ttcagggggt tgagggtcag gcggccgaga
     3421 cgctgggccg tggggtcgcc atacagcttg gccacccaac cgtgagcgac ctcatggacg
     3481 gtaatggcaa acagcagagg cagggcggct accgcaaaaa actggggcag gcttaggttg
     3541 ggcattggct gcggagtata tcaggaccct tcactgactt gaaagccaga atccagcttt
     3601 atccccttga ttttcagaaa gaatcaagcg gcaaaccgcg ccgcgtcgcc cttgcctttg
     3661 cgtaccagcg tcaccgcccc gtcttccagt tcgatcacgg tcgtggggtc caatccgcag
     3721 aaaccgccgt cgattaccaa gtccaactca tgctccagat gggtccggat ttcctccggg
     3781 tcactcaggg gcagatcgtc tcccggcagt atcagggtgg agctcatcag cggttcattc
     3841 aactcggtca acagggactg cgcgatgaca tggtccggga tgcgtaaacc aatggtcttg
     3901 cgcttggggt gctgcaggcg gcgtggcact tcacgcgtgg ccggcaggat gaaggtatac
     3961 ggacccgggg tattggctcg cagcaggcgg taatcggcgt ttcccacccg cgcatactgc
     4021 gccacctcgg aaagatcgcg gcagaccagc gtgaaattat gcgtatcgtc cacgcggcga
     4081 atgcgccgga tgcgttccat cgcctgcttg tcgccgatat ggcaaccgag cgcatagctc
     4141 gaatcggtgg ggtacgcgat cacgcccccg gcgcgcacga tttccaccgc gcgcttgatc
     4201 aggcgcgatt gcggatttac cggatggatt tgaaaatatt gagccataag atttgggtaa
     4261 ccacaaatga acacagataa acacggatcg cggcgaaaac taggccgttt gttggggtta
     4321 ttaatttacc aatctgtgtt catccgtgtt tatcggtggt ttcatattca tattccagtc
     4381 ctgccagaca ggaatgcaac catccggcaa ggccggcaac cttccaagat ccatccaagt
     4441 tttctccgga ccatggtaat ccgatcccgc cgaggccagg aaaccgtgct ccgtggcgat
     4501 accggcaaaa tgccgattgg catcgggcgt gtggctgccg gagatcacct cgatcgcggt
     4561 accgccacat tcgcggaatt cgcccagcag ctgacgcagt ttcccggcgc tcagcttgta
     4621 gcgcgccgga tgtgccacca ccgcctctcc acccgcggaa acgatccatt gcaccgcctc
     4681 ggcgagggtg gcccagcgac cgggcacgtg gccgggcttg ccgcgcgaaa gaaactggcg
     4741 gaaggcctgc tgcggacccc gtacataacc ctcgtcgacc aaaaagcgcg caaaatgcat
     4801 gcgtgaaata atcgttccct tggccaggcg cgccgcgcct tcgtaggcgc cttcgattct
     4861 ttttttctgc aggcggcggc cgatttcctc ggcgcgccac tggcggaatt cgcgcagccc
     4921 gctgaggccc tgctgcagct cgggctggtc ggggcagata ttgaggccga cgatatgcag
     4981 cgtctggttc tgccacgtaa ccgaaatctc cacgcctgcg atcagtttta taccgagccg
     5041 gtccgcggcg ctttgcgcct cggcgatgcc atcggtaacg tcgtgatcgg tcagcgccag
     5101 cacccgcacg cccgcggcgt gcgcgcgctc gaccagctcc gacggcgtga gcgttccatc
     5161 cgagcgcaag ctgtgcgtgt gcatatcgta gccgcgcgac ggaaccggtt cggagattga
     5221 attggaactg ctcatggcaa acagggtatc atgaataatc aattatttcg tgacggcacc
     5281 ctatcgccca tggccatcac cagcctgcca cccacccttg ccgaagcggc gctgcgtttc
     5341 aaacacgcgg cggcggagat caatgacacc tacgaggcaa gacgtccgga caagaccaac
     5401 caggtcacgc cggggcaact gacggaggcc atcgagcagt tcctcaccat catcgagcgc
     5461 atcgatcgcg aggaaggcaa agtcggcccc atcgccaagg atgatgccac gcaactgggc
     5521 gattatggca tgacgctcat cgccgacctc tccgcgtggg ccggtcaact caatctgccc
     5581 gtgccgcgac aggatctgga gatcgtgacg ctcgccgccg ccgactggat cgtgcgtcac
     5641 gacggccaga ttcgcacgct cgaaccggtc gtcaacgcgt tggccaactt cgccaatcgc
     5701 ttgcacgagc ccacgtcgct ggaaccgctg atcaccctga tggggcgcgt ggcccaggcc
     5761 gccgccaatc tcatcaagca ggacctggag aagaacaatc ccggccggcc gtggcgggtg
     5821 ctgcacctga atcgcggcat cgtcgccacg cgcacgcaca atccggaact gatggaaaaa
     5881 gtcttcgacg atctggtgcg ttacctgccg gaggacgcgg ccgccttttt cgccgaaggc
     5941 atgcagcaga tggaggaact cgattatccg cagcatgtcc gacaggtcat gaaccgttat
     6001 ttcgaccgct ggacacgccc ccgcatgcac tgagacagaa aaatctcggg agaaaaccgg
     6061 gatttttccg cgatgttctt gccgcctcgc cgggctgatg ccagaatagc gctccttttg
     6121 tcaccgctgg cacgatgaaa ttcctttacg acctgtttcc gctgctgctg tttttcgcgg
     6181 ccttcaagct gtacgacatc tacgccgcca cggccgtggc catcgccgct tcgttcgtgc
     6241 aggcggggca tttctggctc aagcatcgcc gcttcgagac catgcacgtg gtcacgctcg
     6301 gcgtcatagt ggtgttcggc ggcatgacac tgctgctgca cgacgatacc ttcatcaaat
     6361 ggaaacccac gctggtctac tggattctgt ccgcgctcgt gctcgccagc caatggatcg
     6421 gcaataaaac cgtcatcgag cgcatgatgt cgtcgcaaat cgtgctgccc gcgacggtct
     6481 ggaaacgtct caatctgagc tggggtattt tcttcgccgc gctcggcgcg gtgaacatct
     6541 acgttgcgtt ctattatgga ctcgatcttg atgcagcaac gcgccagagc atctgggtca
     6601 acttcaaggt attcggactg ctgggcatta cgctggtttt cgtcatcgcg caggcattct
     6661 tcatggcgaa gcatgtccac gacaagccgg agaccgacgc atcctcttcc aacaggaccg
     6721 cggactgatg tggtacgtca tcaacgccac cgacaatgaa ggcagtctcg agcgccgtct
     6781 ttccgtgcgc gcccggcatc tcgcgcgctt gcaggaactg cagaacgagg gccgtctgct
     6841 gcttgccggc ccctacccgg cggtggacgg caacgacccc gggccggccg ggttcagcgg
     6901 cagcctgatc gtcgccgaat tcgattcgct ggaagacgcg caatcctggg ccaacgccga
     6961 tccgtatgtc gaggcgcgcg tttatgcgag catcagcgtc aagccgttcc gcaaggtact
     7021 gccctgatgt cgcgtgcaac cctgattgag agccgcttgc gcgaagcctt caatccctcg
     7081 aaaatcgagg tgcgcgatga cagccaccgg cacgccggac acgaaggcgc gaaaagcggc
     7141 ggcggacatt tcaccgtgac catcgtcgcg ccgcaatttc agggcaggaa cgcggtacaa
     7201 cggcaccaga tgatttaccg ggcgctcggc gacatgatga aaaaggaaat tcatgcgctc
     7261 agcatccaag cgctcgcgcc ggacgagctt tgaagcacga atcagtcaaa ccccccaacc
     7321 atcaccaagg aaaatatatg tccagcctac gcacaaccct catcgttctt ctggctgcgg
     7381 tcggcctgtt cgccgccggc tgcgataaaa aaccggccgg cgaggccggc aaagaagcgg
     7441 ccaaggacgc cgacaaggtc tttgccaccg tcaacggcga aaagatcatg ggcgcggatt
     7501 atcagaatta cctgcaactg cgccagcagc aggtcggccc gctgcccgac aaggacaagg
     7561 aaaagaagat cgtgctggat gagatgatcg aaaagatgtt gctggcccag catgccgtca
     7621 gcaccaagct cgaccaggac ccggaagtga gcggcctcat gaagcgcgtg cgcgaggaaa
     7681 tcctggcgca ggccgtaaaa cgcaagctgc tgcgcgacaa ccccatcacc gatgacgacg
     7741 tgaagaagcg cttcgagcag gaagtcgagg ccacgcacaa gaccgaatac aaggtgcgcc
     7801 acattctcgt caaggatgag gccgaggcga aggacattct ggcgcaactg ggcaagggcg
     7861 cgaaattcga caagctcgcc aaggacaagt ccatggacac gcaaagcggc aagaacggcg
     7921 gcgagctcgg atgggtcaac cagggcatgg tggtgccgga gtttttcaac gccgtgatgg
     7981 cgatgaaaaa aggcgcggtc tcgaccgaac ccgtgaagtc cgacttcggc tggcacatca
     8041 tcaaggtcga ggacacccgt cctctcaaga tcccgacctt cgaacagttc atgtcggatc
     8101 agcgcgcccg cgccaacatc tatcgcaaga tgcaggacga caggatcggc aacctggtca
     8161 aggacctcaa gggcaaggcc aagatcacgg ttgactgaac aagctgcacc ccatcccgcc
     8221 gcgcggcggg atgggtcccc cccgcctgat atttcccgat catgaggcat cttttcaaaa
     8281 tcaccggcac gatcgcggca atgctatatg tcgggctcca cagtggactt cttgcggccc
     8341 agaccgacct ggagacgcga caagtcgtac tttcccaaaa tataaaaatc ggcgaccgca
     8401 tcgggcgcat ccgctttctc ggcatgctgc aactgccgga cgtcacgcgc aatgggctgc
     8461 gactgtcgca actctcgggg ctcgcctggg acgacgatga cggcgtgctt tacgccctct
     8521 ccgacaaggg caacctgttc catctgaaac cgcaattcga cggggatttt ctgatgggtc
     8581 tctaccttct gcgcgcggtg ccgctgcaag atcccggaag caacaagtcc ttcatgcgcc
     8641 cgcgcaccga ttcggaaggg ctcgatatcc tgaatggacg caatggacgc aagggcgatg
     8701 ccgagctggt catcagcttc gagcgcgtcc cgcgcatcgg gcgttaccgc cccgacggca
     8761 gcgcactcgg ggattatccg cttcccgagc cgctgagtgg cgcaaaaaac tttaccggcg
     8821 cgaaccgaat gctggagtcc gtatgcgtgg actcctctct gggcgtcatc accgcccccg
     8881 aagagccatt aaagaatgaa cagagcggct ccacgcgtat tttcagtctt tcaagcgggt
     8941 catggcgtta cccggttgct tcgggagacc ggctgacggc catggagtgt ctcggtcaag
     9001 gcgaggtgat ttttctgcaa cagacatacc tgcacgcctt tggccagatt accgtgtcgc
     9061 tcaagcgtgt tcgcctcgca tccaatccat cttccgagcc attgaaaccg gaaacgcttg
     9121 ttacgctgga cagccagcag ggattcgaga tcgacaactt cgagggcctc acccgccaca
     9181 aaggcaggcg tttcttcatg gtgagcgata ataacgatct ttttgtgcag cgaacgctgc
     9241 tgatgtactt cgagttgctg gatgaataat cactggcgcc gctggaactg aatttttatt
     9301 tcaccacacc acggctccgc agagcttccc tcaagctttc ggcccggcgc cggttgacac
     9361 cgaagtccca gtaacctgtc cgtgccgcgg accgaacggc aataacaccc ttgtccattc
     9421 tgagctcaag ttcaagatca tccacaaagc cgaatacagc gctcgtacat tcagcacgca
     9481 aataattgtc cgtggcttgc gttattttcg tgcgcggcag gttgttcaca gcttcccggg
     9541 ccagactcca ggctacatcg gaaggaaaaa taatctcaaa gggctctaca cgatgtgatg
     9601 cgtcagtcgc gtcgctcgaa acgcaattgg gcgaatcagg gcaaggtgaa aggcgctttg
     9661 gcattgttac cgattcagcc acagttgaaa tgccgcacaa atgaatcaga aatacaatga
     9721 ccagaaagtt ccgccataag cgcagcatgt gacacatcac tgtcctaatc ttgaaattat
     9781 tccgaagaat gagtgatatg gattatcggg ccgacaacac cagcaccgca agaccaccgg
     9841 tgatcacggc gatggcaatc agacgcgtgc gcgcatgttc gcgctcgccg aaggcgaaga
     9901 tcgacagcag gcccgcgatc acaatacccg cattggtgaa agctacggca taggcggcgg
     9961 agagatactg caaggcatag atcacaagcc cgtaggcatt gccaatgcaa agtccggccg
    10021 gcaaccagcg ggatacgggc ggcatggcag acggtcgcca tcggcccaga cgtactcgct
    10081 gttcgagact gagcgcgatg aacgaggcca tcaggtcaac gctcaccagt ccgagctggg
    10141 aaccataggt cggcaaactg gataccgcga atttattgct cagggaatac atactggtgg
    10201 cgaacgcggc gatgacggcc cacatcaccg cgcggcggct gtcgccatga cgagcgctgg
    10261 ccgcgagcaa cagcaggccg gcgacggaaa tgagtattcc gagccaacct gacgcggcga
    10321 tcgcttcgcc gaacagcaaa ctgctccaga gcgcgatgag tagtggcgag ctgcgagcga
    10381 tgggataaac cagcgccacc ggtgcgtgcc gataggccgc acccaagacc aggaaataaa
    10441 gactgttggc aagggccgag ataacgagca aaccggccag acccagcgac cagtgagcct
    10501 gggtaatcag cgcgtacagc gaccacggac cgagaacgac gagataaccg atcagcgccc
    10561 accacagaaa acggctgtcg ggatgactgg cgcgcgccag caggttccag gcgacgtgca
    10621 tcagcaccgc cgcggcaagt gccagcgccg cggcggtcat ttcaatcccg cccgccgatc
    10681 aggccgagtt caatcaaaat atgtaggttg gggtgagcgt agtgaacccc aacagaacaa
    10741 accccaacaa acttatcgtt ctccatattt gttgctgttc tcatcctgat tccccacagc
    10801 ccaatccgga ctcacaatgc cttcggtgat aaaccgatgg atacttgaat ggggccaatc
    10861 caccggcctt gccacgtaac cgtgtttcac ggggttatag tgaatgtaat ccacgtgtcg
    10921 ctcatagtcc ttttcatcgc gtatcaagtg ctcccagaag cggcgctgcc agatgcctcg
    10981 ctcaccctta gcgacccggc tcctgttacg ccgctcgccc gccgatagcc ggcgcgagaa
    11041 tccgctcttg atcagcatcc agcgggtgga aaagtccttg tcgttcttcg gtaatgtcca
    11101 gatagcgtgg agatggtctg ggagtacaac catggcatcg atgcgaaacg gatgccggga
    11161 ttgtgcttcg cggattaccg ttcgcaagac atcgacaaga tccaccagca atgtccgctc
    11221 tctatcggca agattgactg tgaagaaata gctggctccg ggaacccctg cgcggcggta
    11281 acgcatgaga gcaataattc aacgaagttg gattggtgtt tgttggggtt cgcgttgctc
    11341 accccaacct acgggttaaa cgtaattcac gacctaatta cgaacctaca ctgattattt
    11401 acaacaagtt gcgtgtaacc cttatcatct ccctcaaaat cccagacagg ctcctcaaag
    11461 caatagtctc gaacagcaag taatttggct ttatgtttca attcatccct gtcctgagat
    11521 tccataataa attctggctg aggtggaatg acgttttcgg gagtaatgat tactttgtaa
    11581 agtttcatga tggctccatt tcacttgaca gtacaatgaa gaaaactcca cttccgagct
    11641 atatgctaga aaggaaagct tcgttaccaa ttcaaaaaca acatcaacat tgaccagaac
    11701 caaaggttca acgtgctaat ctgcactttg cgattggtct ggaatgaacc aaatccttgg
    11761 ccagcgatag tgtatatcca tacccaaaag ctaataaaaa aaccgataaa gctgatccag
    11821 attaacgttt cattcatgac acattttttc tgtagtacgt aggttagggt aagcgtagcg
    11881 aaccccaaca taatccattc tgtgtcgtct tcataacttt tattctcaag cttttgaatt
    11941 catggctgtt ggggttcatt gcattcaccc caacctacat gaacgctatt tactgatcaa
    12001 cttgagaaac tccttctcgt tcagcatcgt tacccccaat tctcgcgcct tgtccgcctt
    12061 cgagcccgga tcttcgccca cgattacgta atcagttttc ttcgacacgc tgccactgac
    12121 tttggcgccc agctcctgca agcgacgctt ggcttcgtca cgactcatgc tggcaagccc
    12181 gccggtaagc acgaaggttt tgcccacaag cgcggaggac tttttcacgc gcacgggttt
    12241 gggccagtgg atgccggcct ttacgagcgc gcgaatgact tcgcggttgt gcttttcgtg
    12301 gaagaaactg gaaatatcct cggccatatt cgggccgatg ccgtggatct gttcaagcgt
    12361 ttcacgtttg gcgtccagaa tatcgtcgag cgattcgaaa tgatccgcca gcaattgcgc
    12421 ggtggcctcg cccacctgcg gaatcccgag tgcgtgcaga aaacgcgcgg gcgtggtggt
    12481 tttgcttttt tcgatctgat ccagcaggtt ctgcgccgac ttgtcagcca tgcgttcgag
    12541 atcggccaat tcatcttttt taagccgata gatatcggcc acggttttca ccaaaccttt
    12601 gtccaccagt tgctgcacca gcttgtcacc caagccctcg atatccatgg catggcgcga
    12661 ggcaaagtgc aggatcgcgc ccatacgttg cgcggaacac accagcccgc ccatgcagcg
    12721 gtgcgccgcc tcttcctcct cgcggatcac ttccgaatcg cagaccggac atttcttcgg
    12781 catgcgccac gcttttgcgc cgcgcgggcg cttgtccttg atcacgccca cgacctcggg
    12841 aatcacatca cccgcgcgcc gcacgatcac ggtatcaccg atgcgcacgt ccttgcgctc
    12901 gacttcgtcc tggttgtgca gcgtggcatg ggtcacggtg acgccgctga cctgcaccgg
    12961 tttcagcacc gccaccggcg tgatcacgcc cgtgcgaccg accgaggcca ggatgtcctc
    13021 gaccacggtg gtttcttcct gcgccggcag cttgtgcgca atggcccaac gcggcgcgcg
    13081 cgcggtgaag cccaactgat cgcgcgaccg caggtcgtcc accttgtaca ccacgccgtc
    13141 aatctcgaac gggagcttgt cgcgtctggc gagaatttcg cggtaatact cgagatactc
    13201 gtccgcgccg tgcaccacgc ggaaaaactc ggtgacacga aagccccagt cctttagatg
    13261 cttcgatact tccgaatatc gtcgcgccac aggttcggag gtctccccca atccccatgg
    13321 aaaaaaactc aaaggacgcg cggcggtcac gcgcgggtcg agctggcgca ggctgccggc
    13381 ggcggcatta cgtggattgg cgaacacctt gccgccctgc tttagctgct cggcgttgag
    13441 atgttcgaaa tccttcttcg gaatcacgac ttcgccgcgc acctccacca ccttgggcca
    13501 acccttgcct tgcagatgca acggcacggt cttgatggtg cgcacgttgg ccgtcacgtc
    13561 ttcgccggtg gtgccgtcgc cgcgcgtgcc ggcctgcatc agcacgccac tctcgtaacg
    13621 caggctcacc gaagtgccat cgaacttggg ttcggcggtg tagtccaccg ccgcctcggt
    13681 ttccagtccc ttgcgcacgc gctggtccca ttcgcgcgcc tcgttatcgt cgaaggcatt
    13741 gtccatcgag gtcatcggga ttttgtggcg cacttcgcca aactcggaca acggcgcggc
    13801 gccgatacgc tgggtcggcg aatcgggcgt gatcagcttg gggtgatcgg attcgatctg
    13861 ctgcaattcg cgcagcagct tgtcgtattg cgcgtcggag atgacgggat tatcgaggac
    13921 gtagtagcgg tagttgtggt aattgatttc cttgcgcaac tcttcggcgc gtttgcgcag
    13981 cttgtccgca ccgctcataa ggtcgcggct tcgttgaaca gcttgagcgc ggtctcgctt
    14041 cccggcggaa tgccgaaggc gcgcatcttt tcctcgattt cctcgatctg gtgatgtatc
    14101 accgcgatgc ccttgtcgct cagcgggcgg cggttctggt cctgcaattt tccgcccagg
    14161 gtgtcggcga tctcccgcgc ggttgtcgcc atccggtcga acaccacgcc cggctggaat
    14221 acacagggca cgctcatgaa aagcgtgagc ccgccggttt cgaatctgtc ccaggcggcg
    14281 ggatcgaaac cgctcgattc atccaggttc gccagactga acagatagcg gctgccgggg
    14341 gcgacttcgt ttttcttgtg gaagatgttc atggcgccca gctccagtcc cgcgtggcgc
    14401 gccgccattt cgatggcgcg gcccggaaag gccgcctccg tgttcggcac cacgtgaatg
    14461 ccggcgatga catcatgggt atcgcaaaac tcctgcaatt ccctggcgcg cgccaagccc
    14521 gcctcgaagg tttccggcag cttcgtcggc cgcgcgagcg cgtcggccag tttcaggccg
    14581 atctgcatga acgcgttaag ctcggattcg tcgatcggcc cgcgtgaatc ggtcagctgg
    14641 atcgccagct tgatatcgtc gtattcggtg ctgcccggat cgagttgcaa atcggaccac
    14701 cgttccgtcc cgtgatggcg cccgtacaga tggcgcgcct tgttgaggtt gtattcgttc
    14761 tgcttgaaaa cggagagcgc cacgtcgcgc atgaccgggc ccgggcccgg caggtcaatc
    14821 acgaaatcga tttttgcatc cggcagatac tgccggtcgg gctcggcgcg gggacgcagg
    14881 cggcgctgta tgcccggctt gaggttgagc ggcatggtcg ccatttcctc aagcacgcgg
    14941 atttccttgc gcaaaatctc atcgcgcggt ttctcgcgcg gcgcgatttc aatatcggaa
    15001 cgcagaacct tgtcgctgcc ttcttcgcgc gtagcgtcca gcagactggg tcgaacgggg
    15061 gcatggtcat ccgggaaacc gcccagatgc acctccgggc gcgggcgcgg acgccgctgc
    15121 cgggacatgt cgtaggcggt cagagcaacg acggcaacaa tgacgattcc gatgaagagc
    15181 agtgcatact gcaggttcat tactgcgcga ccatctccat ggcctgatcc acgtccaccg
    15241 ccaccaggcg cgacacgccc ggttcggaca tggtgacacc gatcaggatg tcggccatct
    15301 ccatgctgat cttgttgtgc gtgatgtaaa ccagctgggt cttgcccgac aggttcttca
    15361 gggtatcgca atagcggatg acgttggcgt catccagcgg cgcgtccacc tcgtccagca
    15421 ggcagaacgg cgccggattg agctggaaga tggagaacac gagcgccacc gccgtgagcg
    15481 ccttctcgcc gcccgagagc aggtgaatgg tgctgttgcg cttgcccggc ggacgcgcca
    15541 tgacggacac gccggtttcg agcaggtcgt tgtcggtcat ttccagatgc gcgctgccgc
    15601 cgccgaagag ctgcggaaag aagccctgga aatattcgtt gacctgatcg aaagtttcct
    15661 tgaaacgcgt gcgcgtctcg cggtcgatct tgcggatcgc ctcttccagc gtggccagcg
    15721 cctgcgacaa atcctcatac tgcttgtcga gataggtctt gcgcgtgttg gcctcctcga
    15781 attcctcgat ggccaccagg ttgatcggcc caaggcgctc gatgcgcgcg gtgacctgtt
    15841 ccagccgcgc ggcccattcg gtttcgttgg cttcgggcgg cagttccgcg cgcaccggtt
    15901 cgacctcgaa gccggattcg cgcagttgat cggcaaccgt gtcgcgtcgt accgccagtt
    15961 cctggcgcgc gacgcgctcg ctttcgtgct gctcgcggat ttcgccggtt ttatgttctt
    16021 cccgcgcgcg cgcctgttcc tgttcgcgca gctgcgtctc aagatcggtc accgcctggc
    16081 gcgcggcgtt caaacgctcc tcgatgccga ggcgcttttg caggaattcg tccagttgct
    16141 ggcgatagcc cggcgtgggg tcctgctcgc tggaaagcgc cagcgaaagc tgctcgcgcc
    16201 gtgcgataag ttgttgcagc tgcccttcga gacgcgcatg gctggcgcgc gtggcctcga
    16261 aggcggtctg ttgcgtttcg cgctcgattt ccaggcgatg cgcgctgtcg cgcgccgcgg
    16321 actcctgtgc gcgcgtctgt tccaccgcct ccagcaggct ctggcgattc tgctgcaatt
    16381 gcgcgcgccg ctgttcgtgt tcaccgccgg tggcctcggc ctgacgcaac aaatcgcccg
    16441 cggcggaaat ctccgcctgg tcgcggccca gctgctcgtt gacttcagcc tgctcgcgct
    16501 cgatctgcgc gcgccgcgat tcgagctgtg acagccgcgc ctgtttgtgt cccagctgtt
    16561 cgcgcagacg cgcgcgttcg cggttgttct cgctgagatc gcgggtgagt tcgtcgcgct
    16621 cgtcctcgag attctgcaac tgcgtatcca gttcggcgat ttcagcttgc aggttgccga
    16681 gcttgccctg ccatccggac aaatccgctt gcaacgtttc gatttcgcgc tcgcgcaata
    16741 gccatccggc gcgcgcgcct ttctcgctgc cgaggctcaa ccagttgcgg cccacccagg
    16801 cgccatcgcg ggtgacgatg gactcgcgcg tggagagcgc ggcgcgcatc accagcgcct
    16861 gttccagcaa ctcggtgata tagactccct cgagcaacgg ggcgagatcg atgtcgccgt
    16921 gcaccttgtc gatcaaggcc gcgcgcgtgg acggttgatg cgtggacgac gtaccgagtt
    16981 cgacaaagga aacctgtgac cggtcgaagc ccgaggccgc cgacgcgatc tgttccaccc
    17041 gcggcacgca cacggcgccg agattgacac caagcacgcg ctccacggcc ttttcccagc
    17101 cggactcgac cttgagcagg cgcgccagac gcggcgcctg atcgagaccc tgaccgcgca
    17161 accattcacc cagcgcggcg tcgctctcac cctgcgccgc cgcctgcaac tcgcgcaacg
    17221 aggccagacg cgcctcgccg ctttgtttct ggccgcgcat ctcggccagt tcgtcatcca
    17281 gctcatcgcg ccggtcacgc gcctcgcgta ggcgcgtttc gatctcgccg atcgagcgct
    17341 cctgtgcctc gcaagagtta tccagttccg ctacctgcgt ggccaaggca ttggccggtt
    17401 ctttttccaa ctccgagccg atggccgcgg cctcctgttc gaggcgtgac tggcgctggc
    17461 gcaactgttc gatatggctt tccagctggc gcatgcgggc ggtctgtacc tcgcgcacgc
    17521 gggccggttc ggccgccagt tcgctgaagg attcccactc gttctgccag tcctgcatgg
    17581 cgcgctcggc ctgcgcgcgg ctctccgcgg cggcatcgcg cgcgcgggcc agttcttcca
    17641 gttgcggtgt cagttcttcc aggcggcgct tcagttcctc catgcgcgtg acgtccgatt
    17701 gcaagtgcgc gctggcctcg ccccaggcgc ggttgatctg ttcctgctcg cgctcctggt
    17761 tttcgcgcgt ctcgcgcgcg tactggatgg cctgttcgag gcgtgaaatc tccgcgccga
    17821 cggaatagaa ctcggcctgc accgcgttga actgatctac tgtttcagtc tgctgggcgc
    17881 ggattttttc gatctcggcc tcaatcccgc gctgtgccgc cagcaccgcg tccagcgcgg
    17941 tttgttgctg cgctaacaac gtgtcctggc tctgcacgcg cgcatcgagc tcgacccagc
    18001 gcagcacgaa caattgcgcg cgcgtcaggc gctcttcctg tttcagttcc ttgtagcgcg
    18061 ccgcggcgcg cgattgccgg tgcaatttcg agagctgcgt ctccagctcc ttgcggatgt
    18121 cctcgacgcg cgtgagattc tcgcgcgtgt gcttgatgcg gctctcggtc tcgcggcggc
    18181 gttccttgta tctggaaacg cccgcggcct cttcaaggaa accacgcaga tcttccggct
    18241 tggcctcgat gatgcgcgtg accatgccct gctcgatgat cgaataggcg cgcggtccca
    18301 ggccggtgcc gaggaagata tcggtgatgt ccttgcgccg gcacttggtc ttgttgaggt
    18361 agtaatcgga ctggccgtcg cgaccggcct cgcgccggat ggagatctcg gcatacttgg
    18421 cgtactcacc gccggcgcga ccttcgctgt tgtcgaagat gagctcgacc gaggccatcg
    18481 ccaccggctt gcgcgcgttg gagccgttga agatgacgtc ggccatggaa tcgccgcgca
    18541 gattcttggc cgacatctcg cccatgaccc agcgcacggc gtctataatg ttggacttgc
    18601 cgcaaccgtt cgggccgacg atgccgatga ggttgccggt gatggggacg gtgatgggtt
    18661 cgacgaatga tttgaagccg gaaagcttga ttgttttaag tcgcatcggg tctcgcgtgc
    18721 tgtcctgtcc gccggcgtaa attaagctac aattttacaa ggattccaaa ccccgccgcc
    18781 agacatgcca acccagccca gcaagaatct ggataacttt cccaatcctc aaccggggcg
    18841 ggattatatc attgagatcg aatgtccgga gttcacttgt ctgtgcccca agaccggtca
    18901 gccggacttc gccacgctgt ccctggagta cgtcccggac cggctgtgcg tggagctgaa
    18961 gtcgctgaag ctttatgtct ggtcctaccg cgacgagggc catttccacg aggacgtgac
    19021 taaccggatt ctgaacgatc tggtagccgt cttgcacccg cgttatctgc ggctgcgggc
    19081 caaattcaac gtgcgcggcg ggctctacac cacagtcgag gtcgtgcacc gccagagcgg
    19141 ctggcaggcc ccaccaccgc caccggccga tctgccgcgc gaggttcagg aactgccggc
    19201 gaaggaagtt ccggccgcgg ctcctgcgaa aaccaccgtc gccaaggcca tgccagcacc
    19261 gaaaacccgc aacccggtga aactgaccga acctgtcatc ccgcccgcgc ccgcgccgaa
    19321 aaatcggaat ccggctccag caccggaacc cgagacaccg gccagcgacc gcttccgcat
    19381 gctgcggcgc tcgcgccgta acgagccgcc accggaaccg gagccggaga tcgtttccgc
    19441 gccggaaccc gaaacggtgg aaccggagcc accgcctccg gcgccggcac gcaaggacgg
    19501 gctctatatc ggtatcgatc tcggcaccac cggctgccgc gccatcgccg tcgacgacgc
    19561 cggcaaggta cagggcgaga tgagcgcccc cattcccacc ccgccccgca ctgaggacga
    19621 ggt
//
LOCUS       DBNGZE010000433        11515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1380220, whole genome shotgun sequence.
ACCESSION   DBNGZE010000433 DBNGZE010000000
VERSION     DBNGZE010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1380220"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..828)
                     /locus_tag="AC16FV_11265"
     CDS             complement(19..828)
                     /locus_tag="AC16FV_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850667.1"
                     /translation="MLGPEVEKEQLFFQATKEDRGWYFVEYRPPISGYQFATLQLVVL
                     EKARALEEIAESMEAELVIWLERYPVPIMVSAFDDSGNLMGLQGHRECNHLIGYIDGQ
                     THKVVREWRLLSNEELPKDALDTSYLRRIYSQIPFKTKQQLREEAQQKAKHLRLGWWI
                     VFVWAVVVPAGVAILEWWSDWLGVVVLLYSLYKAVEKALRLMGKWPKSKAEIQREAEE
                     ARMRHHHYHCERNPEGFERLKAENFERWARDEVREEAQSLKQSARRDGNDG"
     gene            complement(1071..1661)
                     /locus_tag="AC16FV_11270"
     CDS             complement(1071..1661)
                     /locus_tag="AC16FV_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR2850668.1"
                     /translation="MSFCNQIFLFLSALLIAACSPSAPTFKSTDISGVEWGGDFMLTA
                     HTGKPVKASEFNGKVVIMFFGYTHCPDICAPTLVKLDQVVKRLGEDAKNVQVLFITVD
                     PEHDTVQQLAGFIPPFNPSFIGLTGTDKEIAAVASEYKVAYGKNPQSKPGQILVDHST
                     GILVKDRRGKLRLLVKNDVAIDDLEHDIRVLLREKN"
     gene            complement(1770..2717)
                     /gene="cyoE"
                     /locus_tag="AC16FV_11275"
     CDS             complement(1770..2717)
                     /gene="cyoE"
                     /locus_tag="AC16FV_11275"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR2850669.1"
                     /translation="MEPTSKIGPVTAASPRRLRSLAGEYYELTKPRVVSLIVFTAVVG
                     MFLSVPSGLPALPLLLGTLGIALAAGSAAAINQSLDQRFDSGMTRTSFRPLPTGTLTT
                     REALIFALILGVTSMAILVLWVNTLTAVLTFISLIGNSIVYTVWLKHATPQNIVIGGA
                     AGAAPPVLGWTAITGEVGSDALLLFLIIFLWTPPHFWALALYREKEYAKVGIPMLPVT
                     HGREFTQLQILLYTVLLVAVTIMPFATHMSGWLYLAGAVVLNAGFLYYAVRLYRNYSD
                     ELARKTFRYSINYLALLFALLLVDHYRVYIHEALQSALY"
     gene            complement(2817..3428)
                     /locus_tag="AC16FV_11280"
     CDS             complement(2817..3428)
                     /locus_tag="AC16FV_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR2850670.1"
                     /translation="MSESVNQSGARRKILLLAALFALPVVIAYALYFSGWRPASFGNH
                     GELVQPARPIADVTLTLLDGKAIRFGELRGKWALIVFSAAECGSPCERNLVKMRQVIA
                     AQGQEAERIQSVLVMKDARTRDWLNYAMKDYPGMRAVVGPSEAVEALARQFALPVGSP
                     LDNLNRIYLVDPLGNFMMSYPADADGNGIRKDLARLLRISRIG"
     gene            complement(3425..4165)
                     /locus_tag="AC16FV_11285"
     CDS             complement(3425..4165)
                     /locus_tag="AC16FV_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR2850671.1"
                     /translation="MRPARRFRPGLITSIVAALLLSLFLYLGYWQLQRAEEKRQMQAE
                     YDTRANGPAVRVEGRVQRAEELQFYRVVAKGRYETDYQILIDNRVHQGRVGYHVITPL
                     RLQDSEVRLLVNRGWIALGESREHLPVIDTPVDLQQITGVATVPAEKYFTLGKPQPGW
                     QRVWQNMDMARYAASVPFPVQPVVVLLDPADAAGGFTRDWSRLDAGISVHQGYAFQWF
                     MLAAALAGIYLFMSLRRRDVDKQEDEEP"
     gene            4243..4458
                     /locus_tag="AC16FV_11290"
     CDS             4243..4458
                     /locus_tag="AC16FV_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR2850672.1"
                     /translation="MLLKIVVITMLVLILASLFSALVFLFKDKGRGNRTARALTWRIG
                     LSITLFLLLMGGFHFGIIPPDGLHLMR"
     gene            complement(4538..5419)
                     /locus_tag="AC16FV_11295"
     CDS             complement(4538..5419)
                     /locus_tag="AC16FV_11295"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR2850673.1"
                     /translation="MSAAHSGNNYYLPNPSAWPVITSAGLFSLALGFILLMNNIKAGP
                     WVMLLGAAIIIFMMIRWFGQVIGESESGTYNAQVDRSFRWGMAWFIFSEVMFFAAFFG
                     ALFYVRQFAVQWLGADDMLWPGYDAGWPTAGPKGPVPVPHDTVPGLNQFSPMGAWGIP
                     AINTLILLSSGATVTWAHWGLLKDNRAQLITGLFMTVALGITFLSLQAYEYIHAYTEL
                     GLTLKTGVYGATFFMLTGFHGLHVTMGTIMLIVILGRSIVGHFRPDHHFGFEAVAWYW
                     HFVDVVWLGLFIFVYWL"
     gene            complement(5461..6090)
                     /locus_tag="AC16FV_11300"
     CDS             complement(5461..6090)
                     /locus_tag="AC16FV_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR2850674.1"
                     /translation="MTADRDSRAAANRRLAIKLALVTVAMFAFGYALSPMYDLMCKAF
                     GLNGKTGRVEEQAAAQPVDMSRTVTVEFTGLSTSGLPWEFKPLVKKLDVHPGETTEVK
                     YLVRNLAQEEITGQAIPSVAPGVSAAHFKKIECFCFTQQTLKPGETREMPVRFVVDAG
                     LDKNVQTITLSYSFFNTNKLSAKKYGGEALAQDEEHAAHHVHPATASGG"
     gene            complement(6222..7781)
                     /gene="ctaD"
                     /locus_tag="AC16FV_11305"
     CDS             complement(6222..7781)
                     /gene="ctaD"
                     /locus_tag="AC16FV_11305"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR2850675.1"
                     /translation="MATAHHADHPTGLSRWLFTTNHKDIGTLYLIFAFVMAIIGGLMS
                     LVIRSELWQPGLQLVDPNFFNQMTTVHALVMIFGFAMPGFVGLANWQIPMMIGAPDMA
                     LPRMNNWSFWILPFAATLMILGLFMPGGGPSGGWTLYPPLSIQGGIGTDFTLLSIHML
                     GMSSLLGSINIIATILNMRAPGMTLMKMPLFAWTWLITAFLLIAVMPVLAGAVTMLIT
                     DRHFGTHFFDAAGGGDPVLWQHVFWFFGHPEVYILVLPAFGVFSQIIPTFARKPLFGY
                     DSMVFSTASIAFLSFIVWAHHMYTVGMPMAGELFFMYATMLIAVPTGVKVFNWISTMW
                     RGSMTFETPMLWAIGGVFVFTIGGLSGLMLAITPVDFQYHDTYFVVAHFHYVIFGASV
                     FGLFGAITYWLPKWTGHMYSETLGKWHFWLTMIGVNLTFFPQHFTGLAGMPRRIPDYA
                     LQFTEFNQWSTVGAFLSIGAQLIFFYMAIRCMKGGAKATDQVWEGSDGLEWTLPSPPP
                     YHSFTVAPKVK"
     gene            complement(7813..8952)
                     /gene="coxB"
                     /locus_tag="AC16FV_11310"
     CDS             complement(7813..8952)
                     /gene="coxB"
                     /locus_tag="AC16FV_11310"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR2850676.1"
                     /translation="MSMTFVKRLPGVVSRLILGLSLWFTVPAAFAKYGLNFQKPVTAI
                     GHKLLGLHNMIIIICVVIFVIVFGFMFYSIFAHRKSRGHKAAQFHENTTLEVVWTVIP
                     FLILVSMALPSTATLLEMDDTSKSDLTVKITAYQWKWKYDYPDQDIGFFSSLATPRDQ
                     VENKAAKGEHYLLEVDNPIVLPVGKKVRFLVTANDVIHAWWVPQLGVKKDAIPGFINE
                     MWTRIDEPGLYRGQCAELCGKDHGFMPIMVQAVSPEDFTKWVAMQKDKAAAESAGGAK
                     TWSKDELIEKGKKIFGSTCAACHGPNGEGMGPFPKMAGSKIANGPLADHLNIVMNGKA
                     GTAMQAFAAQMNDTDIAAVVTFERNSFGNKAGDMVQPSQIKALRK"
     gene            complement(9027..9515)
                     /locus_tag="AC16FV_11315"
     CDS             complement(9027..9515)
                     /locus_tag="AC16FV_11315"
                     /inference="COORDINATES: protein motif:HMM:NF021517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HIR2850677.1"
                     /translation="MDARFTPEGDTGEGLRVIVRPNRTLTLRGMTVLFAGLTLVVLTV
                     GIGFTLAGAWPVLPFAGLEMAVVGAVLYRLFRHTDDHDLIIVDRERVTVIRRRGRREW
                     RDEFQRYWTKVTLERRRGWYPSQLKVGSHGRFVVIAADVNEEERKTLSATLSNIISQK
                     AG"
     gene            complement(9758..10444)
                     /gene="bioD"
                     /locus_tag="AC16FV_11320"
     CDS             complement(9758..10444)
                     /gene="bioD"
                     /locus_tag="AC16FV_11320"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR2850678.1"
                     /translation="MSGGWFVTGTDTGVGKTLVSRLLLEALRHAGQSAVGMKPVASGC
                     HVTEAGLRSDDALELMKASDISVGYTDVNPYALGSACAPHIAAQEMGVEIELKKILES
                     FRRLQQKSPWVVVEGVGGWLVPLGERLTMADVARALGLPVILVVGLRLGCLNHALLTV
                     EAIRRADIPLAGWIANRIDPAMTHVPENIAALEQKIDAPLLAQFPFEPAMKPGIAASA
                     IPIQMLMRFT"
     gene            complement(10441..11316)
                     /gene="bioC"
                     /locus_tag="AC16FV_11325"
     CDS             complement(10441..11316)
                     /gene="bioC"
                     /locus_tag="AC16FV_11325"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HIR2850679.1"
                     /translation="MTETLALDKRRLRQSFAQAADTYDAVAVLQREIADRLLARLEVV
                     RLVPRVILDLGCGTGYDLRRLSQRYPRAQLLGLDIAETMTRRARRRLGLWRRFTGRSL
                     FACGDAERLPLATASVDMVVSNLALQWCDPPTVFAEARRVLRPGGLLMFTTFGPETLR
                     ELRAAWRAADDAAHVHSFIDMHDLGDMLLRAGFADPVMDMEAFTLTYADVRDVMRDIK
                     QLGAHNVAVSRARGLTGKARFARFRAAYEALAQDGKIPATYEAVYGHAWAPETEPARD
                     GVAAIPVARIGRRRL"
     gene            complement(11309..11476)
                     /locus_tag="AC16FV_11330"
     CDS             complement(11309..11476)
                     /locus_tag="AC16FV_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR2850680.1"
                     /translation="MMLGERDRLVPMGAARFLAEHLPQARLAPVPGAGHAPFLSHPAM
                     FLEKLKSFIDD"
BASE COUNT         2426 a         3519 c         3422 g         2148 t
ORIGIN      
        1 gtcggctgca ttaagggatt agccatcatt tccatccctc cgcgctgact gctttagcga
       61 ttgcgcttct tcacgaacct cgtcgcgcgc ccagcgctcg aaattttccg ccttcaagcg
      121 ttcgaagccc tcgggattgc gctcgcaatg gtagtgatgg tggcgcattc tggcctcttc
      181 cgcttctctc tgtatctcgg ctttcgactt tggccatttg cccatgaggc gtagcgcttt
      241 ttccacggcc ttatacaagc tatatagcaa gacgaccacg cccagccagt cgctccacca
      301 ttcgaggatt gcgacaccgg cgggcacaac cacagcccaa acaaatacga tccaccaccc
      361 gagccgaagg tgctttgcct tttgttgagc ttcctcccga agttgctgct tggttttgaa
      421 tggaatctgc gagtagattc gcctcaaata agacgtgtca agcgcatcct tcggtagttc
      481 ttcgttcgaa agcaagcgcc attcgcgaac gaccttatgt gtttgtccat cgatgtagcc
      541 gattaagtgg ttacattctc gatggccttg gagtcccatt aggttgccac tgtcatcaaa
      601 ggcggaaacc atgattggta cgggatatcg ctccaaccaa atgaccaact ccgcctccat
      661 agactcggca atctcttcca gcgctcttgc cttttcgaga acgacaagct ggagggtcgc
      721 aaattggtat ccggagatcg gagggcgata ctcgacgaag taccagcccc gatcttcctt
      781 cgtcgcctga aagaatagtt gttccttttc gacctccgga cccagcatga tgacggctaa
      841 cgatgattag aggacatatt gactgttatc aacattaata ctgcatcggc tcccatgtga
      901 atcaacagct tacggtaaaa gccaccggag ataggctgca acattgcaag ataacatgca
      961 agaaaccgtc aaaccgccgt ataacatgaa tatgcctgca tggcacaaaa gcgaattgac
     1021 caatagataa tctcacccct catctcccgc aagggggaag gggataaagg ttaatttttc
     1081 tctctcaaca aaaccctgat gtcatgctca aggtcatcaa tagcaacgtc attcttgacc
     1141 agcaaccgta acttccctct cctgtccttc accagaatcc ccgtggaatg atccaccaga
     1201 atctgcccag gcttgctttg ggggtttttg ccgtaggcca ccttgtactc actggccacg
     1261 gcggcgattt ccttgtcagt gccggtgagg cctatgaaag aaggattgaa cgggggaatg
     1321 aaacccgcga gctgttgcac ggtgtcgtgc tcgggatcaa cggtaatgaa caacacctgc
     1381 acgtttttgg cgtcctcgcc gaggcgcttc acgacctgat cgagtttcac cagcgtcggg
     1441 gcacagatgt ccggacagtg ggtgtagcca aagaacatga tcacgacttt cccgttgaat
     1501 tccgaggcct tgaccggctt gccggtgtgc gcggtgagca tgaaatcccc gccccactcg
     1561 acgccgctga tgtccgtgga cttgaacgtt ggcgcggacg gcgagcaggc ggcaataagc
     1621 agcgcactca gaaaaaggaa gatttgattg cagaaactca cgtgaaacat cacatcaatg
     1681 gcggaagcgc gagaagggtg ctcttaattt ttgagcagta tccttggcgc tcttggcgtc
     1741 cttggcggtt caaaacaatt aatcaaacct taatacagcg cggattgcag cgcctcgtgg
     1801 atatagacgc ggtagtgatc aaccagcaat aacgcgaaca gcaacgcgag ataattgatc
     1861 gagtagcgga aggtcttgcg cgcgagttcg tcactgtagt tccggtacag ccgcacggcg
     1921 taatacagga agcccgcgtt cagcaccacc gcaccggcga gatacagcca gccactcatg
     1981 tgggtggcga acggcatgat cgtgaccgcc accagcaaca cggtgtagag caggatctgc
     2041 aactgggtga actcgcgccc gtgggtcacc ggcagcatgg ggatgccgac cttggcgtat
     2101 tccttctcgc ggtacagcgc gagcgcccag aaatgcggcg gcgtccacag gaagatgatc
     2161 aggaacaaca gcaaggcatc ggaaccgact tcgcccgtga tggcggtcca gccaagcacc
     2221 ggcggcgcgg caccggcggc gccgccgatg acgatgttct gcggcgtggc gtgcttgagc
     2281 catacggtgt agacgatcga gttgccaatg agcgagatga acgtgagcac ggccgtgagc
     2341 gtgttgaccc acagcacgag gatcgccatc gaggttacgc ccaggatcag ggcgaagatc
     2401 agcgcctcgc gcgtggtcag cgttccggtc ggcagcgggc ggaaactggt gcgcgtcatg
     2461 cctgagtcga aacgctggtc gagactctgg ttgatggcgg cggcagaacc ggcggcaagc
     2521 gcgatgccga gggttccgag cagcaagggc aacgccggca gacccgaggg cacggacaga
     2581 aacatgccga ccacggcggt aaaaacgatc agcgaaacca cgcgcggctt ggtcagctcg
     2641 taatattcgc cggccaggct gcgcagccgg cgcgggctgg cggcggtgac cggtccgatt
     2701 ttgctcgtgg gttccattgg gcgggctatt ctacgtggtc gggatcgaac atggtataac
     2761 ccagatcaag tcgtccgcgc ggcaaaaacc ctgaaatttc cggcatttca gtcatctcag
     2821 ccgatgcgcg agatgcgcag cagacgcgcc aggtccttgc ggatgccgtt gccgtcggca
     2881 tcggcgggat agctcatcat gaagttgccg agcgggtcca ccaggtaaat gcggttcagg
     2941 ttgtcgagcg ggctgcccac gggcagtgcg aactggcgcg ccaacgcctc gacggcctct
     3001 gatggcccga cgaccgcgcg catgccgggg taatccttca tcgcgtaatt cagccagtcg
     3061 cgcgttctgg cgtccttcat gaccaacacg ctttgaatgc gttcggcctc ctgcccctgc
     3121 gccgcgatga cctggcgcat tttcaccaga ttgcgttcgc aggggctgcc gcattcggcg
     3181 gcgctgaaca cgatcaacgc ccatttgccg cgcaactcgc cgaagcggat cgccttgccg
     3241 tcgagcagcg tcagcgtgac atccgcgatc gggcgcgcgg gttgcaccag ttcgccgtga
     3301 ttgccgaacg aggccggacg ccagccgctg aaatacaatg cataggcgat gaccacgggc
     3361 agggcgaaca gcgccgccag caaaagaatc ttgcggcgtg cgccggattg attgacagat
     3421 tcactcatgg ctcctcatcc tcctgcttgt ccacgtcgcg gcgacgcaga ctcatgaaca
     3481 gataaatgcc ggccagcgcc gccgccagca tgaaccactg aaaggcatag ccctgatgca
     3541 ccgaaatccc ggcatcgagc cggctccagt cgcgggtaaa gccgccggcc gcgtccgcgg
     3601 gatcgagcag caccaccacc ggctgcaccg ggaacggcac ggacgccgca tagcgcgcca
     3661 tgtccatgtt ctgccacacg cgctgccagc ccggctgcgg tttgcccagc gtgaaatatt
     3721 tttctgccgg gacggtggct acgccggtga tctgctgcag atccacgggg gtgtcgatca
     3781 ccggcagatg ttcacggctc tcgccgagcg cgatccagcc gcggttgacc agtagccgca
     3841 cctcgctgtc ctgaagccgc aacggcgtga tgacatgata cccgacacgc ccctgatgca
     3901 cgcggttgtc gatcaggatc tggtaatcgg tttcgtaacg gcccttggca acgacgcggt
     3961 aaaactgaag ctcctccgcg cgctgcacgc gcccctccac ccgcaccgcc ggaccgttgg
     4021 cgcgcgtgtc gtactccgcc tgcatctggc gcttttcctc cgcccgctgc aactgccaat
     4081 aacccagata caggaacagc gacagcaata gcgcagccac gatgctcgtg atcagtcccg
     4141 gccggaaacg tcgtgctggc ctcatgccct ggcaaaaacc gcgcggaaat tcatgggtgc
     4201 atccagtata atgcagtttc cgtcatttcc ccggatatcc cgatgctgct caagatcgtt
     4261 gtcatcacca tgttagtgct cattctggcc agcctgttct ccgcgctggt tttcctgttc
     4321 aaggacaagg ggcgcgggaa ccgcacggcc cgggcgctga cctggcgcat cgggttgtcg
     4381 atcacgctgt tcctgttgct gatgggtgga ttccatttcg gcatcatccc gccggacggg
     4441 ctgcacctga tgcgctgagc accgcgcttc tccgcaatcg aaaaaaaagc gctgccagaa
     4501 atggcagcgc ttttttattg gaggcttgtt cctgaactca gagccagtat acgaagatga
     4561 acaggccgag ccacaccacg tccacgaaat gccagtacca ggccacggcc tcgaagccga
     4621 aatggtggtc cggacggaaa tgacccacga tcgagcggcc caggatgacg atcagcatga
     4681 tcgtgcccat ggtcacgtgc aggccgtgga aaccggtgag catgaagaag gtggcgccgt
     4741 aaacaccggt cttgagcgtg agtcccaatt ccgtgtaggc atggatgtat tcataggcct
     4801 gcaatgaaag gaaggtgata ccgagcgcga cggtcatgaa caatccggta atcagctggg
     4861 cgcggttgtc cttcagcagg ccccagtgcg cccaggtcac ggtcgcgccg gaactcagca
     4921 ggatcagtgt gttgatcgcg ggaatgcccc acgcgcccat gggcgagaac tggttcagtc
     4981 cgggaacggt gtcgtgcggc accggtaccg gccctttggg accggccgtc ggccaaccgg
     5041 cgtcgtaacc gggccagagc atgtcatcgg cgccgagcca ctgcaccgca aactggcgca
     5101 catagaagag cgcgccgaag aaggcggcga agaacatgac ctcggagaaa atgaaccacg
     5161 ccatgcccca gcggaacgaa cggtcgacct gggcgttata ggtgccgctt tcggattcgc
     5221 cgatcacctg gccgaaccag cggatcatca tgaagatgat gatcgcggcc ccgagcaaca
     5281 tgacccaggg gccggccttg atattgttca tcaacagaat gaaaccgagc gccagcgaaa
     5341 acagaccggc cgaggtgatg accggccagg cgcttggatt gggcaagtaa taattgttac
     5401 cggagtgggc agcggacatg cttatatcct ctttctcatt tgtatgaatc gttttggcgt
     5461 ttaaccaccg gacgccgtag ccggatgcac gtgatgcgcg gcgtgttcct cgtcttgcgc
     5521 gagcgcctca ccgccgtatt tcttcgccga cagcttgttg gtgttgaaga acgaatacga
     5581 cagggtgatc gtctgcacgt tcttgtccag gccggcgtcc accacgaacc gcaccggcat
     5641 ctcgcgcgtt tcacccggct tcagggtctg ctgggtgaaa cagaaacatt cgatcttctt
     5701 gaagtgcgcg gcggaaacac ccggggcgac gctcggaatc gcctggccgg tgatctcctc
     5761 ctgcgccagg ttgcggacaa ggtatttcac ttccgtggtc tcgccggggt gcacgtcgag
     5821 ctttttcacg agcggcttga actcccaggg cagaccgctg gtggacagcc cggtgaactc
     5881 cacggtcacc gtgcggctca tgtccacggg ttgcgcggcg gcctgttcct cgacgcgtcc
     5941 ggttttccca ttaagcccga aggccttgca catgaggtcg tacatcggcg acagcgcata
     6001 accgaaagcg aacatggcca cggtcaccag cgcgagcttg atcgccagcc ggcggttggc
     6061 ggcggcgcga gagtcccgat ccgccgtcat gcgagcctca gccaggcggc ggaaacgaac
     6121 atcaccgcca ccagcagcgt caacgccatc accgtgaagt gaatgcgttg ccgccggttt
     6181 ttgtcacgca gggcgtcagc cattgccgtt ctgtatccgt cctatttgac cttcggcgcc
     6241 acggtgaagc tgtggtacgg cggcggcgag ggcaaggtcc attccagacc gtccgagcct
     6301 tcccatacct ggtcggtcgc cttggcgccg cccttcatgc aacggatcgc catgtagaag
     6361 aaaatcagct gggcgccgat gctcaggaac gcgcccaccg tggaccactg gttgaactcg
     6421 gtgaactgca gcgcgtagtc ggggatgcgc cgcggcatgc cggccagccc ggtgaagtgc
     6481 tgcggaaaga acgtcaggtt gacgccgatc atggtcagcc agaagtgcca cttgccgagc
     6541 gtctcgctgt acatatggcc ggtccacttc ggcagccagt aggtgatcgc gccgaacagc
     6601 ccgaacaccg aggcgccgaa gatcacgtaa tggaagtgcg ccacgacaaa gtaggtgtcg
     6661 tgatactgga agtccaccgg cgtgatcgcg agcatgagtc ccgacagacc gccgatggtg
     6721 aacacgaaca cgccgccgat ggcccacagc atgggcgtct cgaaggtcat cgaaccgcgc
     6781 cacatggtcg agatccagtt gaacaccttc acgccggtcg gcaccgcgat cagcatggtg
     6841 gcgtacatga agaacaattc gcccgccatc ggcatgccga cggtgtacat atgatgcgcc
     6901 cacacgatga acgacaggaa cgcgatcgag gccgtggaga acaccatgga gtcgtagccg
     6961 aacagcggct tgcgcgcgaa ggtcgggatg atctgcgaga acacgccgaa cgccggcagc
     7021 accaggatgt acacctcggg atggccgaag aaccagaaca catgctgcca cagcaccgga
     7081 tcgccgccgc ccgcggcgtc gaagaagtgc gtgccgaagt ggcggtcggt gatgagcatg
     7141 gtgaccgcgc ccgccagcac cggcatgacg gcgatcagca ggaacgcggt gatgagccaa
     7201 gtccacgcga acagcggcat tttcatcagc gtcatgccgg gcgcgcgcat gttgaggatg
     7261 gtggcgatga tgttgatcga gcccaggagt gacgacatgc cgagcatgtg gatggacaac
     7321 agggtgaagt ccgtgccgat gccgccctga atcgacaacg gcggatacag cgtccaaccc
     7381 ccggagggcc cgccgccggg catgaacagg ccgaggatca tcagggtcgc cgcgaacggc
     7441 agaatccaga acgaccagtt gttcatgcgc ggcagcgcca tgtcgggcgc gccgatcatc
     7501 atcgggatct gccagttggc caggcccacg aacccgggca tggcaaaacc gaagatcatg
     7561 accagcgcgt gcaccgtggt catctggttg aagaaattcg ggtccaccag ttgcagaccc
     7621 ggctgccaca gctcggaacg gatcaccagc gacatgaggc cgccgatgat ggccatgaca
     7681 aaggcgaaaa tcagatacag ggtgccgatg tccttgtggt tggtggtgaa caaccagcgc
     7741 gaaaggccgg tggggtgatc tgcgtgatgt gcggttgcca tgtttagctt tctcctctcg
     7801 gaattctatg cgttacttgc gcagcgcctt gatctgggac ggctgcacca tgtcgccggc
     7861 cttgttgccg aagctgttgc gctcgaaggt caccacggcg gcgatgtcgg tgtcgttcat
     7921 ctgggcggcg aacgcctgca tcgcggtgcc ggccttgccg ttcatgacga tgttcaggtg
     7981 atcggcgagc ggaccgttgg cgatcttgct gccggccatt ttcgggaacg gacccatgcc
     8041 ctcgccgttg gggccgtgac aggcggcaca ggtcgagcca aagatcttct tgcctttctc
     8101 gatcagctcg tccttgctcc aggtcttggc gccgccggcg gactcggccg cggccttgtc
     8161 cttctgcatg gcgacccact tggtgaagtc ctcggggctg acggcctgca ccatgatcgg
     8221 catgaagccg tggtccttgc cgcagagctc ggcgcactgg ccgcgataga ggcccggctc
     8281 gtcgatgcgg gtccacattt cgttgatgaa acccgggatg gcgtccttct tcacgccgag
     8341 ctgcggcacc caccaggcgt ggatgacgtc gttggcggta acgaggaagc ggaccttctt
     8401 gcccaccggc agcacgatcg gattgtcgac ttccagcaga taatgttcgc ccttggcggc
     8461 cttgttctcg acctgatcgc gtggcgtggc cagcgaactg aagaaaccga tgtcctggtc
     8521 ggggtagtcg tatttccatt tccactgata ggcggtgatc ttgaccgtca ggtcggattt
     8581 gctggtgtcg tccatctcca gcagcgtcgc ggtggacggc agcgccatgc tcaccaggat
     8641 caggaacggg atcaccgtcc agacgacttc cagcgtggtg ttctcgtgga attgcgcggc
     8701 cttgtgtccg cggctcttgc ggtgcgcgaa gatggaataa aacatgaagc cgaagacgat
     8761 cacgaaaatg accacgcaga tgatgatgat catgttgtga agccccagca gtttgtgacc
     8821 gatggcggtg accggcttct ggaagttgag tccgtatttg gcgaacgccg ccgggaccgt
     8881 gaaccacaga cttaaaccca ggatcaggcg ggaaacaacg cctgggagcc gcttaacaaa
     8941 agtcatggac atgcctatct cccgtaatga tgtatatcaa atcgttctga ctgcacgccg
     9001 ggctgtcccg gtccgccgct ctccactcaa cccgccttct gcgaaataat attgctcagg
     9061 gttgccgaca gcgtctttcg ttcttcctcg ttcacgtcgg cggcgatgac gacgaaccgc
     9121 ccgtgcgacc ccactttcaa ttgtgaggga taccagccgc gacgacgctc cagcgtgacc
     9181 ttggtccagt agcgttgaaa ctcatcgcgc cactcgcgcc ggccacggcg ccggatcacc
     9241 gtgacgcgct cgcggtcgac gatgatcagg tcgtgatcgt cggtgtggcg gaaaagccgg
     9301 tacaacacgg cacccaccac cgccatctcc aggccggcga acggcaacac cggccatgca
     9361 cctgccaggg taaaaccaat accaacggta aggacaacga gggtcagccc cgcgaacagc
     9421 actgtcatgc cacgaagggt cagcgtacgg ttcgggcgca cgatcactcg caggccttct
     9481 ccggtgtcgc cctcgggcgt aaagcgtgcg tccatcctca ctcacgccgc cacggaaatc
     9541 cttacatctc ccactgaatc aaaaaatact tgatccagat caagccattt tggcgcgcgc
     9601 aaactatcac atgcgtcaaa tcgccgcaat acgcgattta agcgaaaaac acgggttttt
     9661 cagggaataa aacggatgac aaaatgacag caaccgagtg aatcggtcag ccttacaaag
     9721 tctccctgct tcgcttcgtt tgtctcgaga ctgaagatta agtgaaacgc attaacattt
     9781 ggatcgggat cgccgacgcc gcgataccgg gtttcatggc aggttcaaat ggaaattgcg
     9841 ccagcaaggg cgcatcgatt ttttgttcca gcgcggcgat gttttccggt acatgcgtca
     9901 tggcgggatc gatccggtta gcgatccacc cagcgagcgg aatatccgca cgccggatag
     9961 cctccaccgt cagcaacgca tgattgagac agccgagacg cagaccgacc accagaatca
    10021 cgggcaggcc cagggcgcgc gcgacatcgg ccatggtcag gcgctcgccg agcggtacca
    10081 accagccgcc cacgccctca accaccaccc acggggattt ttgctgcaaa cgacggaaac
    10141 tttcgagaat tttcttcagc tctatttcaa cgcccatctc ctgcgcggcg atatgtggcg
    10201 cacatgccga acccaaggca taaggattca cgtcggtgta accaaccgat atatcgctcg
    10261 ccttcattaa ttccaaggcg tcatcgctgc gcagaccggc ttcggtgaca tgacagccgc
    10321 ttgccaccgg tttcataccc acggcggatt gccccgcatg cctcagcgcc tccagcaaaa
    10381 gacgcgaaac cagcgtcttg cccacgccgg tgtcggtgcc ggtgacgaac cagccgccgc
    10441 tcatagccgt ctgcgcccga tgcgagccac gggaatggca gcaacgccgt cacgcgccgg
    10501 ctcggtttca ggcgcccagg catgtccata aaccgcttca taggtcgccg gaatttttcc
    10561 atcctgcgcc agtgcttcgt aagccgcgcg aaagcgggca aagcgcgcct tgccggtcag
    10621 cccgcgcgcg cgcgaaaccg ccacgttatg cgcgccgagc tgcttgatgt cgcgcatcac
    10681 gtcgcgtacg tcggcatacg tcagcgtgaa cgcctccatg tccatgaccg gatcggcgaa
    10741 gccggcgcgc agcagcatgt cgcccaggtc gtgcatgtcg atgaagctgt gcacgtgcgc
    10801 ggcatcgtcc gccgcgcgcc aggccgcgcg cagttcgcgc aaggtctccg gcccgaaagt
    10861 agtgaacatg agcaggccac cgggacgcaa cacgcgccgc gcctcggcga acacggtcgg
    10921 cggatcgcac cattgcaagg cgagattgga aaccaccatg tccaccgagg ccgtggccag
    10981 cggcagccgc tcggcatcgc cgcaggcgaa caggctgcgg ccggtaaatc gccgccacag
    11041 accaagacgg cggcgggcgc ggcgtgtcat ggtctcggcg atatccagtc ccagcagctg
    11101 cgcgcgcgga tagcgctgcg ataaccggcg caggtcgtag ccggtgccgc aaccgagatc
    11161 gagtatcacc cgcggcacaa gccgcaccac ctccaggcgc gccagcaggc gatcggcgat
    11221 ctcgcgctgc aacacggcaa ccgcgtcgta ggtgtccgcc gcctgcgcga aagactggcg
    11281 caggcggcgt ttgtcgagcg ccaatgtctc agtcatcaat aaaactcttc agtttttcca
    11341 gaaacatcgc aggatgcgac agaaacggtg cgtgcccggc gccgggcact ggcgccaggc
    11401 gcgcctgcgg caaatgctcc gccagaaacc gcgccgcacc catcggcacc agccggtcgc
    11461 gttctcccag catcatcgga cgccattgcc ttgacggagg aagacaagaa gatta
//
LOCUS       DBNGZE010000434         7857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1866204, whole genome shotgun sequence.
ACCESSION   DBNGZE010000434 DBNGZE010000000
VERSION     DBNGZE010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1866204"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..785
                     /locus_tag="AC16FV_11335"
                     /pseudo
     CDS             1..785
                     /locus_tag="AC16FV_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944731.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            876..1943
                     /gene="hemE"
                     /locus_tag="AC16FV_11340"
     CDS             876..1943
                     /gene="hemE"
                     /locus_tag="AC16FV_11340"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR2850681.1"
                     /translation="MVELKNDRLLRALLRQPVDVTPVWIMRQAGRYLPEYRAVREKAG
                     DFKTLCTTPELACEVTLQPLRRFALDAAILFSDILTIPDAMGLELYFTEGEGPQFRKP
                     VRSAADVDALPVPDPEVELRYVMDAVRLIRRELDGNVPLIGFAGSPWTLATYMVEGGG
                     SKEFALVKRMLFDQPALLHRLLDVLARAVTVYLNAQVAAGAQALMLFDTWGGALSPRD
                     YRAFSLQYMERIVRGLTREHEGRRVPVILFTKNGGQWLEAMAATGCDALGIDWTTDLS
                     DARARVGQRVALQGNMDPSILYASTDRIRSEVATVLASYGKGSGHVFNLGHGIHQHAD
                     PEKVAVVVDAVHELSRPYHQA"
     gene            1948..2373
                     /locus_tag="AC16FV_11345"
     CDS             1948..2373
                     /locus_tag="AC16FV_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850682.1"
                     /translation="MDHGRCPFEKAILSAQCACELATRFSVAEQMGVTCRSGIARNNC
                     ITLLALMRERARFALKVTDTSDSLPFGKEMKVMIGGLIGLQGLLTTEQGAARVNNIHA
                     LVREAQEKYRSLESLPYQEIVKSITAFQARRRGHAPPKP"
     gene            2641..3963
                     /locus_tag="AC16FV_11350"
     CDS             2641..3963
                     /locus_tag="AC16FV_11350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2850683.1"
                     /translation="MQDESEASGASSSKKLLGLLEGLKRTPVGAVIYDQVARIVEEQE
                     GVTAKLDHAYASLLHLLLNAYARDPSADHITRINARLIQLRRGPITPRAVGKIPDETI
                     EEKLHKAAEAQIPGRTTPAGAKPEPAPAPSPPPTTPPRADMGATQAERRVDAAYRQHL
                     EQQRSEIEKLQETLAKTVTDAITQNREFGALLQIELRALQQANGDKEIEQLRQILVGG
                     LEELIQGQNSLDTKLHKTGGYLRLIKADSERLQDELNKVRLLSLTDEFTGLPNRRAFM
                     RRLQDEISRSQRYGSSLALAMIDLDEFKAINDIHGHAAGDAILRCYATDVLTVLRHHD
                     LVARYGGEEFAVLLPNTTRDGAVAAIQKVRSRALQAVCEFQGREFRLPTFSTGIALYA
                     TGETHTDLIDRADRALYRAKRLGRNRIELEQGLPPLAPQTRGKSGNGA"
     gene            complement(4031..4108)
                     /locus_tag="AC16FV_11355"
     ncRNA           complement(4031..4108)
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_11355"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            4213..4704
                     /locus_tag="AC16FV_11360"
     CDS             4213..4704
                     /locus_tag="AC16FV_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850684.1"
                     /translation="MADSNTSTSADPSLLQSLKLHLDGLKRTQHGLILYNQIQRGLQK
                     YGHEDGRIELVFVTFLHALLGKYATSPACDPATRVKARLIQQRLTLYLPKDVPAVTKP
                     AVTAPPRPAPVAAKPTAAPPAAPKPPTPPAPVVEAKPEPVPEVVVPPIQASAPRGRTR
                     PVQ"
     gene            4649..5518
                     /locus_tag="AC16FV_11365"
     CDS             4649..5518
                     /locus_tag="AC16FV_11365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2850685.1"
                     /translation="MCRQFRPARHEAEPAPSNEAPPSSRQEKIEALEDTLAEKVTESI
                     AVDKQFGELLENERETLKNLESPLGEFNDLKAVLVKGLNELIEERKALIEKLSSAGDY
                     LKAVESDRERLRQELSHARKHSLADELTGLPKRDVFVKQLEAEIGRVKRYGFALAVAV
                     IDVDNLDSVNKRYGRSAGDAVLRCYAGAILGKFRTYDLVARYGGDEFAVLFPNTQKDG
                     AMHALEKARKTAASTFIQQEGKNIPLPSFSSVLTQYSPGEPASALLERADQTLSQARL
                     TGYNRMVVALPTA"
     gene            5608..6159
                     /locus_tag="AC16FV_11370"
     CDS             5608..6159
                     /locus_tag="AC16FV_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HIR2850686.1"
                     /translation="MRIRIFLAALAAGFSGAVAADSIDINLNNDSIQAIYASNWRTAE
                     FNVGLLSNTDQNDWAASMGLLASGERQTGASRVEGGLGGKIYLADVANQDVLALGLGG
                     EFRTFPNNGPIGFGGYLYYAPNVVTTMDGEKFWEFGARVEFEAVKKTANVYIGYRKMR
                     ADLDNGQDVTVDSGLHAGVKISF"
     gene            6195..>7857
                     /locus_tag="AC16FV_11375"
     CDS             6195..>7857
                     /locus_tag="AC16FV_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HIR2850687.1"
                     /translation="MKSLWSDTEAAQFPGELGQRVYTSRLLGRDKSLVQHGGGNTSVK
                     ITEKNLLGEDETLLYVKGSGWDLEFIEAAGFSPVRIDHLLKLAKLKQLSDPQMVNELR
                     THMTRASAPTPSVEAILHAILPYQYVDHTHADAVITITNTANGLERIREIYGDNVVVV
                     PYVMPGFDLARRCAEQFAADVRKNTIGMVLMNHGIFSFGRTAQESYERMIDLVTWAEN
                     YLTKHKAWVLPESSEKPETKPLHRELAALRRELSVTAGFSVILAAHEDTASLAFARRT
                     DVATISQQGPATPDHVIRTKRLPMIGRDVSAYAEAYKKYFSEHAQKTKEPKTMLDPAP
                     RVILDREFGVATVGRSAKDAAICYDIYAHTLEIIQRATALGGWQALPAKDIFDVEYWD
                     LEQAKLKGGGKPPVFAGEIALVTGAASGIGRACVDSLLARGTAVVGLDIKPAITSLYS
                     RPDYLGLVCDVTDEKQLAEALEKTTRTFGGLDMLILNAGMFPPGRRIDALSTMEWDKV
                     MRLNLDANLMLMREAHPLLKLAPQGGRVVVIGSKNVPAPGPGAAAY"
BASE COUNT         1556 a         2510 c         2448 g         1343 t
ORIGIN      
        1 ccgtcgaaga actgaagaag aatttcgatg cgattctgct tgccggcggt gcctgtgccc
       61 cgcgcgatct caaggccccg ggacgtgaac tcaaaggcgt gcacttcgcc atggagtatc
      121 tgacccaatc caaccgtcgt tgcgaaggtg acaaggttcc tggcgagtag gagatatcag
      181 ccaagggcaa gcatgtcgtg atcatcggcg gcggcgacac cggtgccgac tgtctcggta
      241 ccgcgaaccg tcatggcgcg aagagcgtgc accagctgga aatcatgccg cgcccgcccg
      301 agcaccgggc cgcgaacaac ccctggcccg aatatcccta tatctatcgc gtggcctcgg
      361 cgcacgaaga gggcgtcgag cgggtttatt cggtttcgac cagacgtttc ctcggcgatg
      421 cgcaaggccg cgtgcaggcg ctggagctcg tcaaagtcga gctggtgcag gagggcgggc
      481 gttcgagttt caaggaaatt cagggcagcg agttcacgct gccctgcgag ctggttttgc
      541 tcgccatggg ctttgtcgga ccggagaaac cgggcatgct ggaacgcctc ggcgtgaagc
      601 tcaccgagcg cggcaacgtt tggcgcgacg aaaactggat gaccagcgtg ccgggcgtgt
      661 tcaccgccgg cgacatgcag cgcggccagt cgctcatcgt ctgggccatc gccgaggcgc
      721 gctcggccgc gcgcggcatc gacaaatatc tcacgggcgc ctccgacctc ccggcgcctt
      781 tgtaaaaaga aaatccaccg ccaagacgcc aaggacgcca agaaaagcaa cttcaataaa
      841 tcttggcgct cttggtggcc caaaattatt tttttatggt agagctcaag aacgatcgat
      901 tacttcgtgc actgctcagg cagccggtgg acgtgacgcc ggtgtggatc atgcgccagg
      961 cgggacgcta cctgcccgag taccgcgcgg tgcgcgagaa agccggcgat ttcaagacgc
     1021 tgtgcaccac gccggagctg gcctgcgagg tgacgctgca accgctgcgg cgtttcgcgc
     1081 tggatgctgc gattctgttt tccgacattc tcaccatccc ggacgccatg gggctggaac
     1141 tgtattttac cgagggtgaa ggcccgcagt tccgcaaacc ggtgcgcagc gccgctgatg
     1201 tggacgcgct gccggtgcca gaccccgagg ttgagctgcg ttacgtgatg gacgcggtgc
     1261 gcctgatccg gcgcgagctg gacggaaatg tcccgctcat cggcttcgcc ggcagcccgt
     1321 ggacactggc aacctacatg gtcgagggcg gcggcagcaa ggaattcgct ctcgtcaaac
     1381 gcatgctgtt cgaccagccg gcgctcctgc accgcctgct cgacgtgctg gcgcgggccg
     1441 tgacggtcta tctcaacgca caggtggccg ctggcgcgca ggcgctgatg ctgttcgaca
     1501 cctggggcgg tgcgctcagc ccgcgcgact atcgcgcgtt ctcgctgcaa tacatggaac
     1561 ggatcgtgcg cggcctgaca cgcgagcacg aaggccggcg ggtcccggtg atcctgttca
     1621 ccaagaacgg tggccagtgg ctggaggcca tggccgcgac cggctgcgat gcgctgggga
     1681 tcgactggac cacggacctt agtgatgccc gcgcccgcgt cgggcagcgt gtcgcccttc
     1741 agggcaacat ggatccgtcc attctctatg cctccaccga ccgcattcgc agtgaagtcg
     1801 ccaccgttct tgccagttac ggaaaaggca gcgggcatgt tttcaatctg gggcacggca
     1861 ttcatcagca tgccgatccg gaaaaagtgg cggtcgtggt ggatgccgtg catgaactga
     1921 gccgtcccta tcatcaggcg taacactgtg gaccacggcc gctgcccgtt cgagaaggcc
     1981 atcctgagcg cgcaatgcgc ctgcgaactg gccacccgtt tttccgtggc ggagcaaatg
     2041 ggtgtgactt gccgctctgg catcgcccgt aacaactgca taacgctgct ggccctgatg
     2101 cgcgagcgtg cgcgcttcgc gctcaaggtc acggatacct ccgacagtct tccctttggc
     2161 aaggaaatga aggtgatgat cggggggctc atcggtctgc aaggcctgct gacaacggag
     2221 cagggcgctg cccgggtgaa caacatccat gcgctggtgc gggaggcgca ggaaaaatat
     2281 cgctcgctgg agtcgctgcc ttatcaggag atcgtcaaat ccatcaccgc gttccaggcc
     2341 cggcgccgcg gccacgcgcc ccccaaaccc tgagcccgcg cttaagccgg gttctccggt
     2401 atcggcggta agtctttgat gtgtgttgtt tgcgccgatt tcgccttatg aaacattgtt
     2461 atttggagtg ggcgcgggta aattgacgtc taatccgcac ttatacggat gtcttgtttc
     2521 ggtaagaaga acaagaaaaa caaatcgttc tgcgacagct ccacagggaa aagggtgccc
     2581 gtgtcgcgta tgccggcggc tcgcggacta ccgccgtcca ggcaggaggt ggggtcattg
     2641 gtgcaggatg agtccgaggc gagtggagcg tcgtcctcaa aaaagctgct tggattgctg
     2701 gaaggcctga aacgcacacc ggtcggtgcc gtcatctacg accaggtggc gcgtatcgtg
     2761 gaagaacagg aaggcgtgac ggccaagctg gaccacgcct atgcctcgtt gttgcacctg
     2821 ctcctgaacg cctacgcgcg cgatccgagc gccgatcata ttacccgcat caatgcgcgc
     2881 ctgatccagt tgcgccgcgg cccgatcact ccccgtgccg tcggcaaaat cccggacgag
     2941 accatcgaag agaaactcca caaggcggcc gaggcccaga ttcccgggcg cacaactccc
     3001 gcgggcgcga agcccgaacc cgcgccggcg ccaagtccgc ccccgacaac cccgccgcgc
     3061 gccgacatgg gcgcgacaca ggccgaacgc cgcgttgacg ccgcgtaccg ccagcatctc
     3121 gagcagcagc gcagtgagat cgaaaaatta caggagacgc tcgccaagac cgtgaccgac
     3181 gccatcaccc agaaccgcga attcggcgcg ctgttgcaga tcgaactgcg cgccttgcag
     3241 caggccaacg gcgacaagga aatcgagcag ttgcggcaga ttctggtcgg cgggctggaa
     3301 gagctgatcc agggacagaa ttcgctcgac accaagctgc acaagaccgg aggttatctc
     3361 cggctcatca aggccgacag tgagcgcctg caggacgaac tcaacaaggt gcggctgctg
     3421 tcgctgaccg acgagttcac cggcctgccc aaccgccgcg cgttcatgcg ccggttgcag
     3481 gacgagatca gccgctcgca gcgttacggt tcctcgctcg cgctcgccat gatcgatctg
     3541 gacgagttca aggccatcaa cgatatccac ggtcacgccg ccggcgacgc cattctgcgc
     3601 tgctacgcca ccgacgtgct gaccgtgctg cgccaccacg atctggtcgc gcgttacggc
     3661 ggcgaggaat tcgcggtgct gctgcccaac accacccgcg acggcgcggt ggccgccatc
     3721 cagaaggtgc gcagccgcgc gctgcaggcc gtgtgtgagt tccagggccg ggagttcagg
     3781 ttgcccacct tttccaccgg catcgccctg tatgcgaccg gcgaaaccca caccgatctc
     3841 atcgaccgcg ccgaccgcgc cctgtaccgc gcgaaacggc tgggccgcaa ccgcatcgag
     3901 ctggagcagg gtctgccgcc gctggcgccc caaacccggg gcaaaagcgg caacggtgcc
     3961 tgaatccgcc ttgatgatgc tccggcgggt tgtgaaccgg actttgagcg actaatctcc
     4021 taacgaagct gcctcaaaac cgtcgcgagc ggccgtcaag ccgggtgagg acggagcgca
     4081 ggaaccggag tgtacacggt aagtacatga ggattccgag caccgcccga tcccggatca
     4141 cgaacgcgca gcaggttttg ggcagtttcg tttaaaggca ggcctttcac gcctgcatca
     4201 aaggagatgg ccttggcgga ttccaatact tccacgtcgg cggatccgtc gctgctgcaa
     4261 agcctcaagc tccatctcga tggtctcaag cgcacccagc acggattgat tctctacaac
     4321 cagatccagc gcggtcttca gaaatacgga cacgaagacg gacgcatcga actcgttttt
     4381 gtcactttcc tgcacgcgct gctcggcaag tacgccacga gcccggcctg cgacccggcc
     4441 acgcgcgtga aggcgcgtct catccagcaa cgtcttactc tttatctccc caaggacgtg
     4501 ccggcggtta cgaaacccgc tgtcacggcg ccgccgcgcc cggcgcctgt cgcggcaaaa
     4561 cccacggccg cgccaccggc agcacccaaa cctccgacac ccccggcgcc cgtggttgag
     4621 gcaaaaccgg aaccggttcc ggaggttgtt gtgccgccaa ttcaggccag cgcgccacga
     4681 ggccgaaccc gccccgtcca atgaagcgcc gccatcctcg cgtcaggaaa agatcgaggc
     4741 gctggaggat acgctggcgg agaaggtgac ggaaagcatc gcggtggata aacagttcgg
     4801 tgaacttttg gagaacgaac gcgaaacctt gaagaatctt gaaagcccgc tgggcgagtt
     4861 caacgatctg aaggcggttc tggtcaaggg cctgaacgag ctgatcgagg aacgcaaggc
     4921 gctgatcgaa aaactttcca gcgccggcga ctatctcaag gccgtggaat cggaccgcga
     4981 gcgtttgcgc caggaactca gtcacgcgcg caagcacagc ctggcggacg aactcaccgg
     5041 cctgcccaag cgcgacgtgt tcgtcaaaca gctcgaagct gaaatcggcc gcgtgaagcg
     5101 ctatggcttc gcgctggcgg tggcggttat cgacgtggac aacctcgatt cggtcaacaa
     5161 gcgctacggc cgatccgcgg gcgatgcggt gctgcgctgc tacgccggcg ccatcctcgg
     5221 caagtttcgc acctacgatc tggtggcgcg ttacggcggt gacgaattcg ccgtgctctt
     5281 ccccaacact cagaaggacg gcgcgatgca cgcgctggaa aaggcgcgca agaccgccgc
     5341 cagcaccttc atccagcagg aaggaaaaaa catcccgctg ccgagtttct ccagcgtgct
     5401 cacgcagtac tcgccgggcg agcccgcgtc cgccttgctc gaacgcgcgg accagacgct
     5461 gagccaggcc cgcctgaccg gctacaaccg catggtggta gcgctgccga cggcctgacc
     5521 gcactcgccg ccaaagctgt tgcttctggc cggtctccat ctgatcagtt agtattgccc
     5581 cgcctatcaa atggaggaga acagtccatg cgtattcgga tattcctggc cgcgctggcg
     5641 gcgggtttca gcggcgcggt ggcggccgat tccatcgaca tcaatctcaa caacgacagc
     5701 attcaggcga tttacgccag caactggcgc accgccgagt tcaacgtggg tctgctgtcc
     5761 aacaccgacc agaatgactg ggcggccagc atgggtctgc tggcttcggg cgagaggcag
     5821 accggcgcaa gccgggttga gggcggcctg ggcggcaaaa tttacctggc ggatgttgcc
     5881 aatcaggacg tgctggcgct cggtctcggc ggcgagttcc ggacgtttcc caacaacggt
     5941 ccgatcggct tcgggggtta cctgtattac gccccgaacg ttgtgaccac gatggatggc
     6001 gagaagttct gggaattcgg cgcgcgcgtc gagttcgagg cggtcaagaa aaccgccaat
     6061 gtctatattg gctaccgcaa gatgcgcgcg gacctggata acggccagga tgtcaccgtg
     6121 gattccggtc tccacgcggg agtcaaaatc agcttttagc ttccgcgcct ctctatcatc
     6181 cgcgggcgct tggcatgaaa agtctctgga gcgacacgga agccgcgcag ttcccgggcg
     6241 aactgggaca gcgcgtctac acctcgcgcc tgctcgggcg cgacaagtcg ctggtgcagc
     6301 acggcggcgg caacacctcg gtcaagatca cggaaaagaa cctcctcggt gaggacgaaa
     6361 ccctgctgta cgtaaagggc agtggctggg acctggaatt cattgaagcc gccggtttct
     6421 cgccggtacg catcgatcat ctgctcaagc tcgccaaact caaacagctg tccgacccgc
     6481 agatggtgaa cgaactgcgc acgcacatga cgcgcgcctc ggcgcccacg ccttcggtcg
     6541 aggctatcct gcacgcgatc ctgccgtacc agtacgtgga tcacacccat gccgacgccg
     6601 tgatcaccat taccaatacg gcaaatggcc ttgagcgcat ccgcgagatc tacggcgaca
     6661 acgtggtggt tgtgccctac gtgatgcccg ggttcgatct cgcgcgccgc tgcgccgagc
     6721 agttcgccgc cgacgtgcgt aaaaatacca tcggcatggt gttgatgaac cacggtatct
     6781 tttccttcgg ccgcaccgcg caggaatcct acgagcgcat gatcgatctg gtcacctggg
     6841 cggaaaatta cctgaccaag cacaaggcct gggtgctgcc ggaatcgagc gaaaaaccgg
     6901 aaacgaaacc gctgcaccgg gagctggcag cgctgcggcg cgagctttcc gtcacagccg
     6961 ggttttccgt gattctggcg gcgcacgaag acactgccag cctcgccttc gcgcgccgca
     7021 cggatgtcgc cacgatctcg cagcagggcc cggccacgcc ggaccatgtg atccgcacta
     7081 agcggctacc catgatcgga cgcgatgtga gcgcctacgc cgaggcgtac aagaaatatt
     7141 tcagcgagca tgcccagaaa acaaaagagc ccaagaccat gctcgacccc gccccgcgcg
     7201 tgatcctcga ccgcgaattc ggcgtggcca ccgtcgggcg cagcgccaag gacgccgcga
     7261 tttgttacga catctacgcc cacacgcttg agatcatcca gcgcgccacc gcgctcggcg
     7321 gctggcaggc gctgccggcg aaggatattt tcgacgtgga atactgggac ctggagcagg
     7381 ccaagctcaa gggcggtggc aagccgccgg tgttcgccgg cgagatcgcg ctggtgaccg
     7441 gcgccgcttc cggcatcggc agggcctgcg tggactcgct gctggcgcgc ggcacggccg
     7501 tggtggggct cgacatcaaa ccggccatca ccagcctcta cagccgtccc gactatctcg
     7561 gcctggtgtg tgacgtgacg gacgaaaaac agctggccga ggcgctggaa aaaaccacgc
     7621 gcaccttcgg cggcctcgac atgctgatcc tgaatgccgg catgttcccg cccggccgcc
     7681 gcatcgacgc cctgagcacg atggaatggg acaaggtcat gcggctcaat ctggatgcca
     7741 acctgatgct catgcgcgag gcgcatccgc tgctcaagct cgcgccccag ggcgggcgcg
     7801 tggtggtgat tggctccaag aacgtgccgg cccccggccc gggcgccgcg gcctact
//
LOCUS       DBNGZE010000435         7315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_408146, whole genome shotgun sequence.
ACCESSION   DBNGZE010000435 DBNGZE010000000
VERSION     DBNGZE010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_408146"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /gene="lon"
                     /locus_tag="AC16FV_11380"
     CDS             <1..2212
                     /gene="lon"
                     /locus_tag="AC16FV_11380"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR2850688.1"
                     /translation="IHTIGTIASILQLLKLPDGTVKVLVEGERRAVLQKYLETEDYFS
                     AQVAMLEAMPLTDKEGEALMRSVLNEFDQYVKLNMKIPPEILTSLAGIDDPGRLADTV
                     AAHLSLKIEDKQQILEVQDVRERLEKILGVLESEIDLLQVEKRIRGRVKRQMEKSQRE
                     YYLNEQMKAIQKELGELEEGPNEAEELAKKIEKAGMSKEAREKAESELKKLKMMSPMS
                     AEATVVRNYIDWLVNVPWKKRSKIQKDLSKAEEILEADHYGLEKVKERILEYLAVQQR
                     VVKLKGPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVRDEAEIRGHRRTYLGS
                     LPGKIIQNLSKVKVRNPLFMLDEVDKMAMDFRGDPSSALLEVLDPEQNHAFSDHYLEV
                     DYDLSDVMFVATANTLNIPPPLLDRMEVIRLSGYTEDEKVNIATRYLVPKQLKNNGLK
                     ENELHLSEKTIRDVIRYYTREAGVRNLEREIAKICRKVTKELLLSKDKAEKTISVTPK
                     SLEKYLGVQRFRYGTAEQTNQVGQVTGLAWTEVGGELLTIEATIVPGKGKLTITGKLG
                     DVMQESIQAAMSVVRARATKLGIMPDFYQKHDFHVHVPEGATPKDGPSAGIAMCTAMV
                     SALTKIAVRAEVAMTGEITLRGEVLPIGGLKEKLLAAHRGNLKVVLIPEENRKDLAEI
                     PKNIRDSLDIRPVRWIDQVLEAALEHIPEPLDEAKLAEAAKPPETVDTAAVRTH"
     gene            2445..2717
                     /locus_tag="AC16FV_11385"
     CDS             2445..2717
                     /locus_tag="AC16FV_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR2850689.1"
                     /translation="MNKTDLIDKVAVSTDLNKASAARAVEAVLDTIASTLRQGDSVTL
                     SGFGTFSVSNRSQRTGRNPRTGEMITIPASKAPKFKSGKGLKDALN"
     gene            2746..2821
                     /locus_tag="AC16FV_11390"
     tRNA            2746..2821
                     /locus_tag="AC16FV_11390"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2779..2781,aa:Val,seq:tac)
     gene            2830..2906
                     /locus_tag="AC16FV_11395"
     tRNA            2830..2906
                     /locus_tag="AC16FV_11395"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2864..2866,aa:Asp,seq:gtc)
     gene            3010..4896
                     /locus_tag="AC16FV_11400"
     CDS             3010..4896
                     /locus_tag="AC16FV_11400"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR2850690.1"
                     /translation="MLTTIRDKTQGIIATFIMALIGIPFLLWGINSYFDTGGQINVAK
                     VGGVEISQNAYRSEIDRLRGRVEPKTLDNPQFKQTILESLIDQTLIVQHAEDQGYRIS
                     NAQLAEIIRHQSGFQRDGKFDSALYEALLRREGLTPHEFEKRVREQILTTHVQAGIGE
                     SRIVTQTEVADIARLLAQEREVAYALIGPDTLMAKAVVSGQDIEQYYSTHPEMFRFPE
                     QVRVEYLRLSVGDLNQNQQPTEEDLKKAYAEEAARYTTPEKRRASHILISLPAQPTES
                     QVKEALTKIQDIAKQARAGADFASLAKKHSADSMTAAQGGDLGEIRRGVLPKELEDAA
                     YALKSGELSQPVRSTYGYHLVKLTAYTPEKRKAFADVRKELVEAVRRRQAEERFFDVA
                     EKFRNLVYEQPDSLASAAKALGLEIRKSEWFSPAGGTGIAANPKVVQAAFEPDVLSQA
                     RNSDVIEISADALVAIRVAERRPAGRKPLAEVRPQIERALKQERALQQARQLGEETLQ
                     ELNTGGSLETLARKHGFKYQSPKTLTRRQTAGVDPRIVEAGFRAPRPEGGKPVYDLVD
                     LGEQGYAVLALQRVHDASGKPDNALEQQARALLTLHRGSDYYANYRAGLRQKVEIKTY
                     PDQL"
     gene            complement(4906..5706)
                     /locus_tag="AC16FV_11405"
     CDS             complement(4906..5706)
                     /locus_tag="AC16FV_11405"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR2850691.1"
                     /translation="MGFLAGKRALIVGVASDRSIAWGIAKAMHREGAELAYTYQNDKL
                     KERVEKLAAQTKSDILIPCDVSSDEQIEAVFSHLDDYWEGLDIIVHSVAFALREQLAG
                     NFVDVTTREGFRVAHEISSYSLTALAKAGRAMMQGRKGALLTLSYIGAERSVPNYNVM
                     GLAKASLEANVRYLAQALGLDGIRVNAISAGPIRTLAAAGISDFKKLLDYNDKMAPLG
                     RGVTIDEVGNVGAFLCSDLASGVTGEITYVDGGFNTVAIGLEYGKLLE"
     gene            6019..>7315
                     /locus_tag="AC16FV_11410"
     CDS             6019..>7315
                     /locus_tag="AC16FV_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2850692.1"
                     /translation="MKRPGATRSALLAISLIFSAATAASWNNPYPASEAKANILYSAF
                     RERPKTFDPARAYSSNEYLFIAQIYEPPFQYHYLKRPYTLVPLTAVSVPTPTYLDKQG
                     RRLPHNAPAAAIGYSVYEIRIRPGIYYQPHPAFARDAQGRYLYHALSPQDLAKVYAIG
                     DFPQTGTRELVASDYVYQIKRLAHPKVHSPILSVMNDYIVGIRYYADTLKKEYEKIAG
                     GRDTGVYLDLTQFPLAGVEEVDRYTYRVKIRGKYPQLLYWMAMPFFAPMPPEADRFFS
                     QPGMAEKYLTRDWYAVGSGAYMLTVNTPNRQMVLERNPNYHEELYPAEGEPDDGPAGL
                     LNDAGKPMPFIDKVVFSLDKESIPRWNKFLQGYYDRSAVLPEGFDQAIRFTGEGDTEI
                     SDAMKQKGIRLLTSVAASTYYLGFNMLDPVVGGYTERARK"
BASE COUNT         1580 a         2201 c         2171 g         1363 t
ORIGIN      
        1 catacatacc atcggcacca tcgccagcat tttgcaactg ctgaaactgc ctgacggcac
       61 cgtcaaggtg ctggtcgagg gtgaacgccg cgcggtactg caaaagtatc tcgagacgga
      121 ggattacttc tccgcgcagg tggcgatgct cgaagccatg ccgctgacgg acaaggaagg
      181 cgaggccttg atgcgctccg ttctgaatga gttcgatcag tacgtcaaac tcaacatgaa
      241 gattccgccc gagattctca cctcgctcgc gggcatcgac gatccgggcc gcctagctga
      301 cacggtggcg gcgcatctgt cgctcaagat cgaggacaag cagcagatcc tggaagttca
      361 ggacgtgcgc gagcgcctgg aaaaaattct cggcgtgctc gagagcgaga tcgacctgtt
      421 gcaggtcgag aagcgcatcc gtggacgcgt caagcgccag atggaaaaga gccagcgcga
      481 gtattatctc aacgaacaga tgaaggcgat ccagaaggaa ctgggcgagc tcgaagaggg
      541 cccgaacgag gccgaggagc tggcgaagaa gatcgagaaa gcgggcatga gcaaggaagc
      601 ccgtgagaag gccgagtccg agctgaagaa actcaagatg atgtcgccga tgtcggccga
      661 ggccacggtc gtgcgcaatt acatcgactg gctggtgaac gtgccgtgga agaagcgcag
      721 caagatccag aaggacctca gcaaggcgga ggaaattctg gaggccgatc actacggctt
      781 ggagaaggtc aaggaacgca tcctcgaata cctcgccgtg caacagcgcg tggtcaagct
      841 caagggcccg atcctgtgtc tggtgggacc gccgggcgtt ggcaagactt cgctcggtca
      901 gtccatcgcg cgcgccacga accgcaagtt cgtgcgcatg tcgctgggcg gcgtgcgcga
      961 cgaggccgaa atccgcggcc atcgccgtac ttatctcggt tcactgcccg gcaagatcat
     1021 ccagaatctg tcgaaagtga aggtgcgcaa tccgctgttc atgctcgacg aagtggacaa
     1081 gatggccatg gacttccgcg gcgatccgtc atctgcgctg ctggaagtgc tcgacccgga
     1141 gcagaaccat gccttcagcg atcattatct cgaggtcgat tacgatctgt cggacgtcat
     1201 gttcgtcgcc accgcgaata cgctgaacat tccgcctcca ttgctggacc gcatggaagt
     1261 catccgtctc tcgggttaca ccgaggacga aaaggtgaac atcgccacgc gctacctggt
     1321 tcccaagcag ctcaagaaca acgggctcaa ggaaaacgag ctgcatctgt cggaaaagac
     1381 cattcgcgat gtcattcgct attacacacg tgaggcgggc gtgcgtaatc ttgagcgcga
     1441 aatcgccaag atctgccgca aggtcaccaa ggagctgctg ctcagcaagg acaaggcgga
     1501 aaagacgatc tccgtcaccc ccaagagcct ggaaaaatac ctgggcgtgc agcgcttccg
     1561 ttacggcacc gccgagcaga ccaaccaggt cggtcaggtg acgggtctgg cgtggaccga
     1621 agtgggtggc gaattgctca ccatcgaggc caccatcgtg cccggcaagg gcaagttgac
     1681 catcaccggc aagctgggcg atgtcatgca ggaatccatc caggcggcca tgagcgtggt
     1741 gcgcgcgcgc gccaccaagc ttggcatcat gccggatttc taccagaagc atgatttcca
     1801 cgtacacgtg cccgagggcg ccacgcccaa ggacggcccc agtgccggca tcgccatgtg
     1861 cacggccatg gtctcggcgt tgaccaagat cgcggtgcgc gccgaggtgg ccatgacggg
     1921 cgagatcacg ctgcgcggcg aggtgctgcc catcggcggg ctcaaggaga agctgctcgc
     1981 ggcgcatcgc ggcaacctga aggtggtgct gatcccggaa gagaaccgca aggacctcgc
     2041 cgagatcccg aagaacattc gtgacagcct cgacatccgc ccggtgcggt ggatcgatca
     2101 ggtgctggag gcggcgctgg agcacatacc cgagccgctg gacgaagcca agctggcgga
     2161 ggcggcgaag cctcccgaga ccgtcgacac cgccgccgtc cggacgcact gaccggcgcg
     2221 gaggagtctt cccttcgccc tcgatcgagg gtcatgtatc tgttgtctct gacgtccgcg
     2281 gcgcggtgtt ttgataaacc gcgccgcgca tatctttctt gacaaggttt ttccacactt
     2341 gtataaaacc tgcgacgcgt cttggttttt ttccggcgcg ccgtttggtc tcagcagcaa
     2401 tggcttttcg ggcatccatt caccacaact ggcaaggagc taacgtgaat aaaaccgatc
     2461 tcattgacaa agttgctgta tccaccgacc tcaacaaggc ctccgccgct cgcgccgtgg
     2521 aagcagtgct ggacaccatc gccagcacac tgcgccaagg cgactccgtg acgctttccg
     2581 gtttcggcac gttctcggta agcaaccggt cgcagcgtac cggacgcaat ccgagaaccg
     2641 gagagatgat caccatcccg gcctcgaagg cgcccaaatt caagtctggc aaaggcctga
     2701 aggatgcctt aaactagccg cccctttggg ggtagattcg cctccgggtg cttagctcag
     2761 ttggtagagc gtcgccctta caaggcgaat gtcgggggtt cgaacccctc agcacccacc
     2821 aagagaccgg gagcggtagt tcagtcggtt agaataccgg cctgtcacgc cgggggtcgc
     2881 gggttcgagt cccgtccgct ccgccactat aaaatgcatg acgggcgagg ctaaatactc
     2941 gccctttcat tttggttttt gctcgcgcac cgaactacta caccctcgat aaagttaagt
     3001 agcaaattca tgctgaccac catacgcgat aaaacccaag gcattatcgc caccttcatt
     3061 atggcgctga tcggcatccc gtttttgctg tggggcatca actcctattt tgataccggg
     3121 ggacaaatca atgtcgccaa ggtcggaggc gtcgagatca gccagaatgc ctaccgtagc
     3181 gaaatagatc ggctgcgcgg gcgcgtggaa cccaaaaccc tggataatcc ccagttcaaa
     3241 cagaccattc tggaaagcct gatcgaccag acattgatcg tccagcatgc cgaagatcag
     3301 ggttaccgta tcagcaacgc gcaattggcg gagataatcc gccatcagtc gggtttccaa
     3361 cgcgacggga agttcgattc ggccttgtat gaggccttgc tgcgtcgtga aggcctgacc
     3421 ccgcatgaat tcgaaaagcg ggtgcgtgaa caaatcctga cgacccatgt ccaggccggt
     3481 attggcgaga gccgcattgt cacccaaacc gaggtagcgg atattgcccg cttgttggca
     3541 caggagcggg aagttgccta cgccctgatt ggccccgaca cactgatggc caaggcggtg
     3601 gtcagcggcc aagacatcga gcaatactat tcgacgcatc ccgagatgtt ccggtttccc
     3661 gaacaggtgc gcgtcgagta tctgcgtctt tccgtcggcg atctgaacca gaaccagcaa
     3721 ccgacggaag aagaccttaa aaaggcttat gcggaggagg ctgcccgcta cacaacgccg
     3781 gaaaagcgcc gcgccagcca tatcctgatt tcccttcccg cccagcccac cgagagccag
     3841 gtcaaggagg cgctcaccaa gatccaggac atcgccaaac aggcgcgcgc cggcgccgat
     3901 tttgcgtctc tggcgaagaa acattccgcg gacagtatga cggccgcgca gggcggtgat
     3961 ctcggcgaga ttcggcgcgg cgtgttgcca aaggagctgg aagacgcggc gtacgccctg
     4021 aagtccggcg agctcagcca gcccgtgcgc agtacctatg gctatcattt ggtgaaattg
     4081 accgcctaca cgccggagaa acgcaaagcg tttgccgatg tgcgcaagga gctggtcgag
     4141 gcagtgcgtc gccgtcaggc cgaagagcgc ttctttgatg tggccgaaaa attccgcaac
     4201 cttgtttatg agcagccgga tagtcttgcg tccgctgcca aggcgctggg gctcgagatc
     4261 cgcaagagcg aatggttttc tcccgccggc ggcaccggca tcgcggccaa ccccaaggtg
     4321 gtgcaggctg ctttcgagcc ggatgtgctg agtcaggcgc gcaacagcga cgtcattgaa
     4381 atcagcgccg acgcgctcgt ggccatacgc gttgccgagc gccgtccggc cggacgcaag
     4441 ccgctggcgg aagtgcgtcc ccagatcgag cgtgctttga aacaggaacg cgcccttcag
     4501 caggctcgcc aattgggtga ggagacgttg caggaattga acaccggtgg ttcgctcgaa
     4561 acgctcgcca gaaaacacgg cttcaaatac cagtcaccga agacgcttac gcgccggcaa
     4621 accgccggcg tcgatccgcg catcgtggaa gccggcttcc gcgcgccgcg gccggaaggc
     4681 ggcaagcccg tttacgatct ggttgacctg ggtgaacagg gatacgccgt gctggcgctg
     4741 cagcgcgtgc acgacgcctc aggcaaaccg gacaacgcgc tggagcaaca ggcccgcgca
     4801 ttgcttacct tgcatcgcgg ctctgattat tacgcgaatt accgcgcggg tctgcggcag
     4861 aaagtggaga tcaagactta ccccgaccag ctctgagcag ccggtctatt cgagcagctt
     4921 gccgtattca agaccaatgg cgacggtgtt gaagccgccg tccacatagg tgatctcgcc
     4981 ggtcacgccg gaggcgagat ccgagcacaa gaaggctccc acattcccca cctcgtcgat
     5041 ggttacaccg cgcccgagcg gcgccatctt gtcgttgtag tccagcagtt tcttgaagtc
     5101 gctgatgccg gcggccgcca gcgtacggat cggcccggcg gaaatggcat tgacgcggat
     5161 gccatccagg ccaagcgcct gcgccaggta acgcacgttc gcttccagac tcgccttggc
     5221 cagacccatg acattgtaat tgggcacgga acgctccgcg cctatatagg acagtgtcag
     5281 cagggcaccc ttgcgaccct gcatcatggc gcggccggcc ttggccagcg cggtcaggct
     5341 gtaggagctg atttcgtgcg cgacgcgaaa tccctcgcgc gtggtaacgt cgacgaagtt
     5401 cccggctagc tgctcgcgca gggcgaaagc gacggagtgc acgatgatat cgagcccttc
     5461 ccagtagtcg tcgagatgcg agaatactgc ctcgatctgc tcgtcgctgg agacgtcaca
     5521 gggaatcagg atatcggact tggtctgggc cgccagtttt tcgacgcgct ccttcagctt
     5581 gtcgttctgg taggtatagg cgagttcggc gccttcccgg tgcatggcct tggcgatgcc
     5641 ccaggcgatg gaccggtcac tggccacacc cacgatcagc gcccgcttgc ctgccagaaa
     5701 acccattaac gtagccctgc tcgctgcgcg agctcgtcct gacatatgac gggtagctct
     5761 gctcgcaggg ttaggccgtg ctgatatatg actgatagcc ctgcttcgct cgtcttgtta
     5821 ttcatatgct tctccacctg ttattggttc gaatccacag ccggatacgc tgtcgtcagc
     5881 gcaagcatcc ggaagatatt tgccgctaca gtagcgaaaa ttggcgttcg cgggaagagg
     5941 gacggagcgg aggaatttgg tatattggcg gcataaacgg cactcacagg acatggttgg
     6001 agaacggaat caggaagcat gaaacgcccc ggcgcgacgc gctctgcctt gctggcgatt
     6061 tccctgattt tctccgccgc taccgcggct tcctggaaca atccctatcc ggcgtcggaa
     6121 gcgaaagcga atatcctgta ttccgccttc cgcgaacgcc ccaaaacctt cgatcccgcg
     6181 cgcgcctaca gctccaacga atatcttttc attgcgcaga tctacgaacc gccgtttcag
     6241 tatcattatc tcaagcgtcc gtacactctg gttccgctca cggcggtttc tgttccaacg
     6301 ccgacttatc tcgacaagca gggacggcgg ctgccgcaca acgccccggc cgcggcaatt
     6361 ggttacagcg tgtatgaaat ccgcatccgc cccggcattt actatcagcc gcatccggcc
     6421 ttcgcgcgcg atgcccaggg ccggtatctc taccatgcgc tgagcccgca ggacctcgcc
     6481 aaggtatatg ccattggcga ctttccacag accggcacgc gcgagctggt ggcaagcgac
     6541 tacgtgtacc agatcaagcg tctcgcgcat cccaaggtgc attcgccgat cctgagcgtc
     6601 atgaacgatt acatcgtggg gatccggtat tacgccgaca cgttgaaaaa ggaatacgaa
     6661 aaaatcgcgg gcgggcgcga taccggtgtt tatctcgatc tcacgcaatt tccgctcgcc
     6721 ggtgtcgaag aggtggaccg ctatacctat cgcgtgaaga tacgcggcaa gtatccgcaa
     6781 ctgttgtact ggatggccat gccgtttttc gcccccatgc cgcccgaggc tgaccgattt
     6841 ttctcgcagc cgggcatggc ggagaagtat ctcacgcgcg actggtatgc cgtcggcagc
     6901 ggcgcctaca tgctgacggt caacactccc aaccgccaga tggtgctcga gcgcaacccc
     6961 aactatcacg aagagcttta ccccgccgaa ggcgagccgg atgacggccc ggccggattg
     7021 ctgaacgacg ccggcaaacc catgcccttc atcgacaagg ttgtgttcag cctcgacaag
     7081 gaaagcattc cgcgttggaa caaattcctg cagggctatt acgaccgctc cgcggtgttg
     7141 ccggaaggtt tcgaccaggc gatccgtttc accggcgagg gtgacaccga gatcagcgac
     7201 gcgatgaagc agaaggggat tcgattgttg accagcgttg ccgcctcgac ctattacctc
     7261 ggtttcaaca tgctcgaccc ggttgtcggc ggctataccg agcgcgcacg caaac
//
LOCUS       DBNGZE010000436         4358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_825098, whole genome shotgun sequence.
ACCESSION   DBNGZE010000436 DBNGZE010000000
VERSION     DBNGZE010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_825098"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="AC16FV_11415"
     CDS             <1..1598
                     /locus_tag="AC16FV_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HIR2850693.1"
                     /translation="QMLLRGQNLLGYSHYSDDVVEAFVKKAAQNGVDVFRIFDAMNDM
                     RNIQVSVKAVLKAGKHAQGAICYTTSPVHTIEHFVEIAREMEEMGCQSIAIKDMAGLI
                     TPARTVELDKALSKAVKVPLAFHSHTTVGVANICMYLAVEHGARHVDTAISSLSWGTS
                     HSPTESMVVALRETPYDTGLNLERLQEIGFYFREVRKKYHQFESEYAEIDTRVQVSQV
                     PGGMISNLSNQLKEQGALNRMNEVLAEIPHVREDLGFPPLVTPTSQIVGTQAVLNVLT
                     GKRYKSITNEVKLYLQGRYGKAPGRINETVRSMAIGNEEVIACRPADKLKPEMHRLRD
                     EIGELARSEEDVLTFAMFPEIGRKFLEERAAGTLKPEELKPPKSAEAAALPAYAPTDF
                     NVTMHGETYHIRITGAGHKTEEQRPFFVSVDGVPEQILIETLTETVPTEAGVVDTRRA
                     SRGSKRPKASKEGHVTSSMPGTIVDILVKAGVKVKAGDPVLVVEAMKMENEVPAPVAG
                     TVKAVNVTKGDSVNPDEALVEIE"
     gene            1715..2290
                     /locus_tag="AC16FV_11420"
     CDS             1715..2290
                     /locus_tag="AC16FV_11420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR2850694.1"
                     /translation="MKLILASSSPYRRELLERLQIPFEVMAPKVDETPHPGETPEKLV
                     ERLAIEKAQKIAGKTPGTLVIGSDQVAVYNGSIVGKPHSHDKAVEQLRSASGRTVTLY
                     TGLALVNADTRRVQCEVIPYRVTFRALTDVQIETYLRKEQPYSCAGSVKSEGLGIALL
                     EKFEGDDPNTLIGLPLIRLVRMLENEGVKII"
     gene            complement(2416..3396)
                     /locus_tag="AC16FV_11425"
     CDS             complement(2416..3396)
                     /locus_tag="AC16FV_11425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR2850695.1"
                     /translation="MTDDTPSTSASGAEGRSPHWERDLLERLAFAALTEQRRARRWGI
                     FFKLFFAAYLVLILVLAQSDSWSGKGLTTNHTALVDLEGVISADSLANADNVISGLRA
                     AFENKSTQGIVLRANSPGGSPVQAGYINDEIKRLRAKYPKIPLYAVIGDMCASGCYYV
                     VAAADKIYADKASIVGSIGVLMNGFGFVDTMKKVGVERRLLTAGEHKGFLDPFGPVKP
                     AEAKYAQQMLDEIHRQFIETVRQGRGKTLKETKDMFSGLFWTGEEAIKMGLVDSLGGA
                     SYVAREVIGAEEIVDYTYRENIFDRFARRFGTAMAQTIGADVLSRTPVLK"
     gene            complement(3407..3781)
                     /locus_tag="AC16FV_11430"
     CDS             complement(3407..3781)
                     /locus_tag="AC16FV_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR2850696.1"
                     /translation="MRPVICRSRELTERGRGIRFTVRRHGEAAAAFVIRFGGAVHGYL
                     NRCAHRSLELDWSEGDFFDAFGEHLLCATHGARYAPASGACVGGPCGGAGLVKLPVIE
                     KNGVVLLEFVDDIHLVNSEDDA"
     gene            complement(3748..>4358)
                     /locus_tag="AC16FV_11435"
     CDS             complement(3748..>4358)
                     /locus_tag="AC16FV_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR2850697.1"
                     /translation="FTAALDDVGVPHPGEDATRHVIGLGLAEAVATLLPRTDAGTRAQ
                     VVERYREHFLHIDQTDMPLFPGVRQGLESLAAQGYLLAIATGKARRGLDRVLRDTGTA
                     HLFCSTRCADEAFSKPHPRMLEDILEQTGFSATQALMVGDTTYDMQMARHASMDSLAV
                     TYGVHGRELLAEHGPLACLDSFSEVYAWLQQACAPSSAVAAN"
BASE COUNT          979 a         1355 c         1247 g          777 t
ORIGIN      
        1 tccagatgct gctgcgcggc cagaacctgc tcggctacag ccactactcc gacgacgtgg
       61 tcgaggcctt cgtaaagaag gcggcgcaga acggcgtcga cgtgttccgc atcttcgatg
      121 ccatgaacga catgcgcaac atccaggtct cggtcaaggc cgtgctcaag gccggcaaac
      181 acgcgcaggg cgcgatctgc tacaccacga gcccggtgca caccatcgag catttcgtcg
      241 aaatcgcgcg ggaaatggaa gaaatgggct gtcagtccat cgccatcaag gacatggccg
      301 gactgattac gccggcgcgt accgtggaac tcgacaaggc cctgtccaag gccgtgaaag
      361 tgcctctcgc ctttcactcg cacaccacgg tgggcgtggc caacatctgc atgtatctcg
      421 cggtcgagca cggcgcgcgc cacgtcgaca cggcgatctc ttcgctgtcc tggggcacca
      481 gccactcgcc gaccgaaagc atggtggtcg ccctgcgcga aaccccctac gacaccgggc
      541 tcaatctcga gcggttgcag gagatcggtt tctatttccg cgaggtacgc aagaaatatc
      601 accagttcga gagcgaatac gccgagatcg acacgcgcgt gcaggtgagc caggtgccgg
      661 gcggcatgat ctcgaacctg tcgaaccagc tcaaggaaca gggcgcgctc aaccgcatga
      721 acgaagtgct ggcggaaatt ccgcacgtgc gcgaggacct cggcttcccg ccgctggtca
      781 cgccgacgtc gcagatcgtc ggcacccagg cggtgctcaa cgtactgacc ggcaagcgct
      841 acaagagcat caccaacgag gtaaaacttt atctccaggg tcgctatggc aaggcgccgg
      901 gcaggatcaa cgaaaccgtg cgcagcatgg ccatcggcaa cgaagaggtc atcgcctgcc
      961 gtccggcgga caagctcaaa cccgagatgc atcgtctgcg cgatgaaatc ggcgaactcg
     1021 ccaggagcga ggaagacgtg ctgaccttcg ccatgttccc ggagatcggg cgcaagttcc
     1081 tcgaggaacg cgccgccggc acgctcaagc cggaggaact caagccgccg aaatctgccg
     1141 aggccgccgc cctgcccgcc tacgcgccga cggatttcaa cgtcaccatg cacggcgaga
     1201 cgtatcacat ccgcatcacc ggcgccggcc acaagaccga ggaacagcgt ccgtttttcg
     1261 tttcagtcga cggcgtgccg gaacagattc tcatcgagac actcaccgaa accgtgccga
     1321 ccgaggccgg cgtggtggac acgcgtcgtg cctcgcgcgg atcgaagcgc cccaaggcgt
     1381 cgaaggaagg ccatgtcacc agctccatgc ccggcaccat cgtggacatc ctggtcaagg
     1441 ccggcgtcaa ggtaaaggcc ggcgacccgg tgctggtcgt cgaggccatg aaaatggaaa
     1501 acgaagtccc ggcgccggtc gcgggcacgg tcaaggcggt gaacgtcacc aagggcgaca
     1561 gcgtcaatcc cgacgaggcc ctggtcgaga ttgaataaag aaaagcaatc gacaggattt
     1621 acaggattaa aagcaagatt aacaggatta aaattctaaa attaatcctg cgaatcctga
     1681 gcaatcctgt taatcctgtc tattttttgc cttcatgaaa ctcatcctcg cctcgtcctc
     1741 gccctaccgt cgcgagctgc tggagcgcct gcaaattccc ttcgaagtaa tggctcccaa
     1801 ggtggatgaa acgccgcatc ccggcgaaac gccggaaaag ctggtggagc ggctggccat
     1861 tgaaaaagca cagaagatcg ccgggaaaac acctggcacg ctcgtaatcg gttcggatca
     1921 ggtggctgtg tacaacggca gtatcgtggg caagccgcac agccatgaca aggccgtgga
     1981 acagctgcgc tccgcttccg gcaggaccgt cacgctttat accggcctcg cgctggtcaa
     2041 cgccgatacc cgccgcgtgc aatgcgaggt cattccttat cgcgtgacct tccgcgcgct
     2101 gacggacgta cagatcgaaa cctacctgcg caaggaacag ccatactcct gcgccggcag
     2161 cgtcaagtca gaaggattgg ggattgcgct gctggaaaaa ttcgaaggcg acgatccgaa
     2221 tacactgatc ggtctgccgc tcatccgtct ggtgcggatg ctcgaaaacg aaggagtaaa
     2281 gattatataa attaggaccg cagataaacg cagataaaaa atgaaaatga agatgaacgc
     2341 agattgatca aattctgatt tccaggaatc tgtgtttatc tgcgcattta atctgcgttt
     2401 atctgcgttc caaaattatt ttagaaccgg ggtgcgactg aggacatcag cgccaatcgt
     2461 ctgcgccatg gcggtgccga aacgccgggc gaaacggtca aaaatgtttt cgcgataggt
     2521 gtagtcgaca atctcttccg cgccgatcac ctcgcgcgcg acgtaactgg cgccacccag
     2581 gctgtccacc agccccatct tgatcgcctc ctcgccggtc cagaaaagtc ccgagaacat
     2641 gtccttggtt tccttcagcg tcttgccacg accctggcgc accgtttcga tgaattgccg
     2701 gtgaatctca tccagcatct gctgggcgta ctttgcttcc gccggcttca ccggcccgaa
     2761 gggatcgaga aaccccttgt gctcgcccgc ggtcaagaga cggcgctcca cgccgacttt
     2821 cttcatggtg tccacgaatc caaaaccatt catgagcaca ccgatggagc cgacaatact
     2881 ggccttgtcg gcgtaaatct tgtccgccgc cgcgaccaca taataacaac cggaggcgca
     2941 catatcgccg atcacggcat acaacggaat cttcggatac ttggcacgta aacgtttaat
     3001 ctcatcattg atgtaacccg cctgcaccgg gctgccaccg ggactgttgg cgcgcaagac
     3061 aatcccttga gtcgacttgt tttcaaacgc cgcgcgcaat ccggagatga cattgtcggc
     3121 atttgccagg ctgtcggcgc tgatcacgcc ttccaggtcg accaacgccg tatgatttgt
     3181 tgtcaatccc ttgccgctcc agctgtccga ctgggccagc accagaatca acaccagata
     3241 ggcggcgaag aacagcttga agaaaatgcc ccagcgtcgc gcgcggcgct gttcggtgag
     3301 cgcggcaaac gccaggcgtt cgagcagatc gcgttcccag tgcggggagc ggccctcggc
     3361 tccagatgca gaagtggaag gagtatcgtc agtcatggaa atagcctcat gcatcgtctt
     3421 ctgaatttac caagtgtata tcatccacga attcaagcag taccacgccg tttttttcga
     3481 ttacaggaag ttttaccagt cctgcgccac cacagggccc acccacgcat gcaccgctgg
     3541 cgggagcgta acgcgcaccg tgggtggcgc acagcaggtg ctcgccaaag gcatcgaaaa
     3601 agtcgccctc actccagtcg agttccaaag accggtgcgc gcaacggttg agataaccgt
     3661 gcacggcgcc tccgaagcgg atgacgaagg cggcagccgc ctcgccatga cggcgcactg
     3721 tgaaacgaat cccgcgtcca cgctcggtca gttcgcggct acggcagatg acggggcgca
     3781 tgcctgttgc agccatgcat agacctcgct gaaggaatcc agacaggcca gcgggccgtg
     3841 ctccgcaagc agctcgcgtc catgcacacc gtaagtcacg gccagactgt ccatgctggc
     3901 atggcgcgcc atctgcatat cataagtggt atcgcccacc atcaacgcct gcgtcgcgct
     3961 gaagccggtc tgttcgagaa tatcctccaa catgcgtgga tgcggcttgg agaatgcctc
     4021 gtcggcgcag cgtgtggagc agaaaagatg cgccgtgccg gtatcgcgca acacgcggtc
     4081 gagcccgcgg cgcgccttgc cggtcgctat cgccagcaga taaccctgcg ccgcaagcga
     4141 ctccagtccc tggcgcacgc cggggaagag cggcatgtcg gtctggtcga tatgcagaaa
     4201 atgttcgcga taccgttcca ccacctgcgc gcgcgtgccg gcgtccgtcc ggggcaacaa
     4261 cgtcgctacc gcctcggcca gaccgaggcc gatgacgtgg cgcgtggcat cctcgccggg
     4321 atgcggcacg cctacgtcgt ccagcgccgc cgtgaaac
//
LOCUS       DBNGZE010000437         4640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1935569, whole genome shotgun sequence.
ACCESSION   DBNGZE010000437 DBNGZE010000000
VERSION     DBNGZE010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1935569"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..716
                     /locus_tag="AC16FV_11440"
     CDS             63..716
                     /locus_tag="AC16FV_11440"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR2850698.1"
                     /translation="MADGRYPILAREGRWHIALAAGAAVVVHYLAGVAWAAPFWILLV
                     FILQFFRDPPRSIPDAPGAVICPADGKVIALGEVEDPYLKRPARRVSVFMNVFNVHSN
                     RSPAAGKVMERWYSAGKFVNAALDKASLDNERNALWIRTDAGQDIVVVQVAGLIARRI
                     LCYVQPGARIARGERYGFIRFGSRVDVYLPRDAQFEVSLGDKVKGATDVIARMAQEK"
     gene            716..1498
                     /gene="pssA"
                     /locus_tag="AC16FV_11445"
     CDS             716..1498
                     /gene="pssA"
                     /locus_tag="AC16FV_11445"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR2850699.1"
                     /translation="MDHLDETPAPEEKRRRRGIYILPSLFTTASLFAGFYAIVQAMNG
                     NFEYAPLAIFIAAILDALDGRIARLTHTESDFGAEYDSLVDMVSFGLAPALIMYEWAL
                     SGMGKIGWLAAFIYTAGAGLRLARFNTQVGVADKRYFRGLPSPAAAAAVVGLVWVLHV
                     NGVPGKEISIAALIVTVMAGVLMVSNIRYRSFKDVDLRGRVSFMTILAMPLVLVLLFQ
                     DPPLVLFLTFLAYALSGPLGFLFRRVHHETKDHPEKHPHGRV"
     gene            1778..3325
                     /locus_tag="AC16FV_11450"
     CDS             1778..3325
                     /locus_tag="AC16FV_11450"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR2850700.1"
                     /translation="MNDQLIIFDTTLRDGEQSPGASMTRDEKVRIAKVLERMRVDIIE
                     AGFPIASPGDFEAVKAVAEAVTESTVCGLARAVEKDIDRAAEAIKPAKRGRIHTFIAT
                     SPIHMQHKLRMTPEQVLERAVQMVKRARQYTDDVEFSAEDAVRSDIDFLCRVFEAAIN
                     AGARTINVPDTVGYSIPQVWGERIKTLIERVPNSDKAIWSTHCHNDLGLAVANSLAAV
                     LNGARQVECTINGLGERAGNASLEEIVMAVRTRRDIFPCDTRLDTTQIVPASRLVSNI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVIKKRETYEIMRAEDVGWSANRMVLGKHSGR
                     NAFKTRLKELNIEFKTEQELNAAFDRFKDLADKKHEIFDEDLQALVTEADLEQENEHF
                     RLVSLKVCSQTGETPFANVTLAVNGSEKQAGAPGSGPVDAAFKAIDNVAGGQGQLLLY
                     SVNNITSGTDSQGEVTVRLERGGRIVNGHGADTDIVIASAKAYLNALNKLIAPAERTH
                     PQVGQPI"
     gene            3366..4070
                     /locus_tag="AC16FV_11455"
     CDS             3366..4070
                     /locus_tag="AC16FV_11455"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR2850701.1"
                     /translation="MNAVESRRRQYLDALGITRWEPRQAPVSIVPETAVEPVLSKAPD
                     DAGNARQWTELEAEVRVCTKCSLHKTRTQTVFGVGHRHAKWMFIGEAPGADEDRQGEP
                     FVGKAGQLLNAILFAMGLKREEVYIANVLKCRPPGNRDPQPEEVAQCEPYLLRQIELI
                     KPKLIVALGRHAAHSLLKTEVPLGKLRGQKLSYHGTPLFVTYHPAYLLRNPADKRKVW
                     DDLCRAKAAMTKMQES"
     gene            4067..4564
                     /gene="rimI"
                     /locus_tag="AC16FV_11460"
     CDS             4067..4564
                     /gene="rimI"
                     /locus_tag="AC16FV_11460"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR2850702.1"
                     /translation="MSAVVISNQPLLRPMNGHDLVAVMEVEDAAYEFPWTLPIFRDCL
                     RVGCHCYVYESPQEGIIGHGIMSVAVGECHILNICIHPDYQRLGLGEGMVLFLLDIAR
                     QNKARVALLEVRMSNTAAYRLYTKLGFDEVGLRKSYYPARTGREDAIILARDLTVDKP
                     FREGF"
BASE COUNT          904 a         1378 c         1444 g          914 t
ORIGIN      
        1 aaaaacttgg cgttcttggc gtcttggcgg ttcaaaatca attaatcata tttccttaat
       61 ttatggctga cggccgctat cctattctgg cccgcgaggg tcggtggcat atcgcgctgg
      121 ccgcgggcgc ggcggtggtc gtgcattacc tggccggtgt cgcgtgggca gcgcctttct
      181 ggatactgct cgtctttatt ctccagttct tccgtgaccc tccccggagt attcccgacg
      241 cgcccggcgc ggtgatctgt cccgcggatg gcaaggtgat cgcgctgggg gaagtcgagg
      301 acccctatct caagcgcccg gcccggcgtg tgagcgtttt catgaacgtt ttcaatgtcc
      361 attccaaccg cagcccggcg gccggcaagg tcatggaacg ctggtacagc gcgggaaaat
      421 tcgtcaacgc ggcgctcgac aaggcctccc tcgacaatga gcgcaatgcc ctgtggatcc
      481 ggaccgacgc gggccaggat atcgtggtgg tgcaggttgc cggtctgatt gcacggcgta
      541 tactctgtta tgtacagccg ggcgcgcgga tcgcgcgcgg cgaacgttat ggattcatcc
      601 ggttcggatc gcgcgtcgat gtctatctgc cacgggatgc gcaattcgag gtatcgctgg
      661 gtgacaaggt gaagggcgcg acagacgtga tcgcccgaat ggcgcaggag aaatgatgga
      721 tcatctcgac gaaacacccg cccccgagga gaagcgacgg cgtcggggta tttatatcct
      781 ccccagtctg ttcacgacgg ccagcctgtt cgccggtttc tatgccattg tgcaggcgat
      841 gaacggcaat ttcgaatatg caccgctcgc cattttcatc gccgccatcc tggacgcgct
      901 cgacggacgc attgcgcgcc tgacgcacac ggaaagcgat ttcggcgccg agtacgacag
      961 cctggtggat atggtgtcct ttgggctcgc gcccgcgctg atcatgtacg agtgggcgct
     1021 ctcgggcatg ggtaagatcg gctggctggc ggcctttata tacaccgctg gcgccggtct
     1081 gcgcctggcg cgtttcaata cccaagtggg cgtggcggac aagcgctact tccgtgggtt
     1141 gccgagcccg gcggccgcgg cggcggtggt cggtctggtc tgggtgctgc atgtcaatgg
     1201 cgtgcccggg aaggagatca gcatcgcggc gctgatcgtg accgtcatgg ccggcgtgct
     1261 gatggtgagc aatatccgtt accgcagttt caaagacgtg gacctgcgcg ggcgggtgtc
     1321 attcatgacc attctcgcga tgccgttggt gctggtgctg ctgtttcagg atcccccact
     1381 ggtgctgttc ctgacctttc tcgcgtatgc gctgtcgggt cccttggggt ttctgttccg
     1441 gcgggtgcac cacgaaacca aggaccatcc cgaaaaacac ccccacggcc gggtttaaat
     1501 ttcccccgga ttgatttaag ctgtgattat gcagggtcaa tactccatct ccttctgcat
     1561 ccaggcgcac cgccatgcgg tggcgtcaga tcttgctcct gcctctctgc tgtccctgct
     1621 gctgcgccgc taggcgcaca ttaacccccc ttcctggcga ggaataaaat acgcgaccaa
     1681 gaacctgttt tcagcccgcg tgattcgagc gaatcatgtc agcaatggcg aatgacagga
     1741 caagcgaagc agggcaacat tacgcagaga gaacaccatg aacgatcagt tgatcatatt
     1801 cgacacgacg ctgcgcgacg gcgagcagag ccccggcgcc tccatgacgc gcgatgagaa
     1861 ggtgcgcatc gccaaggtgc tggagcgcat gcgcgtggac atcatcgaag ccggtttccc
     1921 gatcgccagc cccggcgact tcgaggcggt caaggccgtg gccgaggcgg taacagagag
     1981 cacggtgtgc ggcctggcgc gcgccgtgga gaaggacatc gaccgcgccg ccgaggccat
     2041 caagccggca aaacgcggac gcatccacac cttcatcgcc accagcccga tccacatgca
     2101 gcacaagctg cgcatgaccc cggagcaggt gctggagcgc gctgtgcaga tggtcaagcg
     2161 cgcccgccaa tacaccgacg acgtggagtt ctcggccgag gatgcggtgc gttccgacat
     2221 cgatttcctg tgtcgcgtat tcgaggcggc gattaacgcc ggcgcccgta ccatcaatgt
     2281 gccggatacc gtcggctaca gcatcccgca ggtgtggggc gagcgtatca agaccctcat
     2341 cgaacgcgtg ccgaactcgg acaaggcgat ctggtccacg cactgccaca acgatctcgg
     2401 actggcggtg gccaactcgc tggcggcagt gctcaatggc gcgcgccaag tggaatgcac
     2461 tatcaatggg ttgggcgagc gcgccggcaa tgcttcgctc gaggagatcg tcatggcggt
     2521 acgcacgcgc cgcgacattt tcccgtgcga cacccggctc gacaccaccc agatcgtgcc
     2581 ggcctcgcgc ctggtgtcga acatcaccgg ttttccggtg cagccgaaca aggccatcgt
     2641 cggagcaaat gcgttcgcgc atgaatcggg catccaccag gacggcgtca ttaagaaacg
     2701 cgagacctac gaaatcatgc gcgccgagga tgtcggctgg agcgcgaatc gcatggtgct
     2761 cggcaaacac tccgggcgca atgccttcaa gacccgcctc aaggaactca atatcgagtt
     2821 caagaccgaa caggagctga acgccgcgtt cgatcgtttc aaggacctgg ccgacaagaa
     2881 acatgaaatc ttcgatgaag acttgcaggc tttggtaacc gaggcggatc tggaacagga
     2941 gaatgaacat ttccgcttgg tgtcgctcaa ggtttgttcc cagaccggag agacgccttt
     3001 tgccaacgtg actctggcgg ttaatggatc ggaaaagcag gccggcgcac cgggcagcgg
     3061 tccggtggac gccgcgttca aggcgatcga caatgttgcc ggcggtcagg gccagctgct
     3121 gctctattca gtcaacaaca tcaccagcgg caccgattcg cagggcgagg tgacggtgcg
     3181 actggaaaga ggcgggcgca ttgttaacgg ccacggggcg gacaccgata tcgtcatcgc
     3241 ctcggccaag gcctatctta acgccttgaa caagctgatc gccccggcag agcgcacgca
     3301 tccgcaggtg ggccagccga tatgacgcgc ttgtgtttcc atgtctctac cttatgcttt
     3361 aggtcatgaa cgccgtggaa tcacgccgtc ggcaatatct cgatgccctg ggcattaccc
     3421 gctgggagcc acggcaggct ccggtgtcaa tcgtcccgga aacagccgtc gaaccggttt
     3481 tgtccaaagc gccggacgat gctggcaatg cccggcagtg gacggagctg gaagccgaag
     3541 tgcgcgtgtg cacaaaatgc tccctgcaca agacgcgcac ccagacggtt ttcggcgtcg
     3601 gtcaccgaca tgccaaatgg atgttcatcg gcgaagcgcc gggcgcggac gaggaccggc
     3661 agggcgagcc gttcgtgggt aaggctggtc aattgttgaa cgcgatcctg tttgcgatgg
     3721 ggctcaaacg cgaggaggtc tacatcgcca atgtgctcaa gtgccggccg ccgggaaacc
     3781 gcgatccgca gccggaagag gtagcacaat gcgaaccgta tctgctgcgc cagattgaat
     3841 tgatcaagcc gaagctcatc gtggcgctcg ggcggcacgc cgcgcacagc ctgctcaaga
     3901 ccgaagtccc gcttggaaaa ttgcgcgggc aaaagttgtc ttaccatggc acaccgttgt
     3961 tcgtaacgta tcatccggcc tacctgttgc gtaacccggc cgacaagcgc aaggtgtggg
     4021 acgatctgtg ccgcgccaag gccgcgatga cgaagatgca agaatcatga gcgccgtcgt
     4081 catctcaaac cagccgttgc tgcgtcccat gaacgggcat gatttggtgg cggtgatgga
     4141 ggtggaggat gccgcctacg aatttccctg gaccctgcct attttccgcg actgtctgcg
     4201 cgtgggttgc cactgttacg tctacgaatc accgcaggaa ggcattatcg gccatggcat
     4261 catgtcggtg gccgtgggcg aatgccatat cttgaatatc tgcatccatc cggattatca
     4321 gcggctggga ttgggggagg gcatggtgct gttcctgctg gatatcgccc gccagaacaa
     4381 ggcgcgtgtg gcgctgctcg aagtgcgcat gtccaacacc gccgcctacc ggctttacac
     4441 caagctcggc tttgacgagg tcggcctgcg caaaagctat tacccggcgc gcaccggtcg
     4501 cgaggatgcc atcatcctgg cgcgggacct gaccgtcgac aagccattcc gggaaggttt
     4561 ctagattctc tgtgcctcga tagcccggcc ggtgctactt gccgttgcct agggttttcg
     4621 atattccgtc tccgcttgcg
//
LOCUS       DBNGZE010000438         5528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_548480, whole genome shotgun sequence.
ACCESSION   DBNGZE010000438 DBNGZE010000000
VERSION     DBNGZE010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_548480"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /gene="rplL"
                     /locus_tag="AC16FV_11465"
     CDS             <1..299
                     /gene="rplL"
                     /locus_tag="AC16FV_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR2850703.1"
                     /translation="METKFGVSAAAVAVAAPAAAAAAAPAEAKDAFDVILAASGDNKV
                     AVIKAVRAVTTLGLKEAKDLVEGAPQTVKEGVPKADAEKMKKELEAAGAKVELK"
     gene            469..4562
                     /gene="rpoB"
                     /locus_tag="AC16FV_11470"
                     /pseudo
     CDS             469..4562
                     /gene="rpoB"
                     /locus_tag="AC16FV_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            4637..>5528
                     /locus_tag="AC16FV_11475"
     CDS             4637..>5528
                     /locus_tag="AC16FV_11475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628202.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR2850704.1"
                     /translation="MKDLFNLFKQPGKSEDFDAIRIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIDLASPVAHIWFLKSLPSRMGLMLDMTLRDIERVLYFEAYVVIDPGMTPLERASLL
                     SEDAYLNAIEQYGDEFDARMGAEAVRDLLRAINLEEEVAKLRAELADTTSETKIKKIT
                     KRLKVLEAFLYSGNKPEWMVLEILPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELGAPDIIIRNEKR"
BASE COUNT         1180 a         1676 c         1708 g          964 t
ORIGIN      
        1 agatggaaac caagttcggc gtctccgccg ctgccgtagc cgtcgcggct ccagccgctg
       61 ccgcggccgc cgccccggcc gaggccaagg acgcgtttga tgtgattctg gccgcctcgg
      121 gtgacaacaa ggtcgccgtc atcaaggcgg ttcgcgccgt gaccaccctg ggcctgaagg
      181 aagccaagga cctggtcgaa ggtgcgccgc agaccgtgaa ggaaggcgtg ccgaaggccg
      241 acgccgagaa gatgaagaag gaactcgagg ccgccggcgc caaggttgaa ctcaagtaac
      301 ctggtgcagg ttggcggttc atccgttgtc ggcgggtgaa tctgtgcgca gtccatgcgg
      361 gttgcgccgg cggtgtaatg ccgccggcgc tgtcccgggt gttgtttttt tatgtcattg
      421 gtcgtaccgg tccgtgacgc tgtttctctt atagggtatc gaggaaacat gacctactca
      481 ttcaccgaga agaaactcat ccgcaagaac tttggcaaac gccctggcgt tctgaaggtt
      541 ccctatctgc tcgaaatcca gaaggaatcc tatcgccagt atttgcaggc ggaaacgccg
      601 ccggagcagc ggatcgacca gggattgcag gccgccttca agacggtgtt tccgatcgag
      661 agttacaacg gcaacgcggc gctggaattc gtcagctatc gcctcggcat accggcgttc
      721 gacgtcaagg aatgccagca gcgcggactg atctacgcgg cgcccctgcg cgccctgctg
      781 cgcctggtgg tgttcgagaa ggacgaggcc accggcacca agaccgtgcg cgacatcaag
      841 gaacaggaag tgtacatggg cgaaatcccg ctcatgaccg acaacggcac gtttgtcatc
      901 aacggtaccg agcgcgtcat cgtttcccag ctgcaccgtt cgccgggcgt gttcttcgag
      961 catgactacg gcaagacgca ttcgtccggc aaactgctgt tctcggcgcg cgtgattccc
     1021 tatcgcggct cgtggctcga tttcgagttc gaccccaagg attatctgta cgtgcgcatc
     1081 gaccgccggc gcaagctcgc cgccacgatc ctgttgcgcg ccatcggcat gtcacgcagg
     1141 aaatccttgc cacctttttc gagaccgaca cctttcatct ggtgaagaac gaaattcggc
     1201 tcgacctgat ccccaaccgc ctgcgcggca tgcagttcga attcgacgtg aaaacgccga
     1261 agggcgaggt catcatcgag gccggccggc gcatttccgg acgtcatatc cgcgagctgg
     1321 aaaaatccaa gctcaagcag ctgaccatcg aaccgagttt cctcatcggc cgctcgctgg
     1381 ccgaggacgt gattcatccg cagaccggtg aactgctggc gcaggccaac gacctggtgt
     1441 cgaaggacct gatcgacaag ctgttcgagg ccggcatcgg ccaggtcaag acgatttata
     1501 ccaacgatct cgatcagggt tcgttcattt ccgacaccct gcgcctagac cccacgcgcg
     1561 atgcgttgga agcgcagatc gaaatttatc gcatgatgcg tcccggcgag ccgcccacgc
     1621 gcgacgccgc ccagacgctg ttcaacaacc tgttcttcag ccccgaccgt tacgacctgt
     1681 ccgcggtcgg ccgcatgaaa ttcaatcgcc gcgttgggcg cgggtccgac gagggcccgg
     1741 gcatcctgac caaggatgac atcgtcgatg ccatgaagat tctcatcgag ctcaagaacg
     1801 gcaagggcga gatcgacgac atcgaccatc tcggcaaccg ccgcgtgcgt tccgtcggcg
     1861 agttggcgga aaaccagttc cgcgtcggcc tggtgcgcgt cgagcgcgcc gtgaaggagc
     1921 gcctgtcgtt ggcggaatcg gaaggcctga tgccgcagga gctgatcaac gccaagccgg
     1981 tatcggctgt catcaaggaa tttttcggca gctcccagct ctcgcagttc atggaccaga
     2041 ccaatccgct gtccgagatc acgcacaagc gccgcgtctc ggcgctcgga cagggcggcc
     2101 tgacgcggga acgcgccggc ttcgaagtgc gcgacgtgca tccgacgcat tacgggcgcg
     2161 tgtgcccgat cgaaacgccg gaaggtccga acatcggcct catcaactca ctcgcggttt
     2221 atgcccgcac caatgaatac ggcttcctcg aaacgccgta tagcaaggtc aacaatggca
     2281 aggtggcgga cgaggtgaat tacctctcgg cgatcgaaga aagcaaattc atgatcgcgc
     2341 aggcgaacgc cacgctcgac agccgcggcc acctgataga cgaactggta tcgtgccgct
     2401 tcaagaacga gttcatgttg tccacgccgg acaaggtgga gtacatcgac gtggcgccgt
     2461 cgcagatcgt gtcggtggca gcctcgctga ttccattcct cgagcacgat gacgcaaacc
     2521 gcgccctcat gggttcgaac atgcagcgtc aggccgtgcc ctgcctgcag accgaaaagc
     2581 cgctggtcgg taccggcatc gagcgcatcg tggcgatcga ttcgggcgcc acggtggtgg
     2641 cgaagcgcgg cggtctggtg gattccgtcg atgcctcgcg cattgtggtg cgcgtcaacg
     2701 acgacgagac caaaccgggc gaggtcggtg tcgacattta caacctgacc aaatacacgc
     2761 gctcgaacca gaacaccaac atcaaccaga agccgctggt gaaagtgggc gacatgatcg
     2821 ccaagggtga cgtgctggcc gacggccaga gcaccgacat gggcgagctg gcgctggggc
     2881 gcaatatcct ggtcgcgttc atgccgtgga acggttacaa cttcgaagac tcgatcctca
     2941 tttccgagcg cgtggtggag aaggattgct ttaccacgat tcacatcgag gaactgcaga
     3001 cggtggcgcg cgacaccaaa ctgggccctg aggaaatcac gcgcgacatc ccgaacgtgg
     3061 gtgagaccgc attggccaag ctcgacgaat ccggcatcgt ttacatcggc gccgaagtcg
     3121 acgccggtga catcctggtc ggcaaggtaa ctccgaaagg cgaaacccag ttgaccccgg
     3181 aagagaagct gctgcgcgcg atcttcggcg agaaggcctc ggacgtgaag gacacctccc
     3241 tgcgtgtacc ctccggcatc tccggcacgg tcatcgacgt gcaggtgttc acgcgcgaag
     3301 gcgtggagaa agacgcgcgc gccaagagca tcgaggagtc cggactggcg agcatccgca
     3361 aggacctcga cgaccagtac cgcatcctgg aaaacgatgc gttcgcgcgc ctggagcgcc
     3421 aactcatcgg caaggtcgcc gacggcggtc cggcgggcct gaagaacggc gacaagatca
     3481 ccaagagtta tctcgacgag ctgccgcgca acaagtggtt cgatatccgc ctcaagaacg
     3541 aggaggcgtc gcaggcgctg gagcagatcc gcggttacct cgccaagcag aaggacctgt
     3601 tcgaccagaa gttcacagag aagaaggtga agctcacctc gggcgacgat ctcgctccgg
     3661 gcgtgctcaa gatggtcaag gtctatgtcg ccgtgaagcg ccgcctgcaa ccgggcgaca
     3721 agatggccgg tcgccacggc aacaagggcg tcatctcgcg catcgttgcg gtcgaggaca
     3781 tgccctacac ggcagatggt acccccgtgg atatcgtgtt gaacccgctc ggcgtgccct
     3841 cgcgcatgaa cgtgggccag gtgctggaaa cccatctcgg ttgggcggcg catgaactgg
     3901 gcgcccaaat cggcaagatg ctggaagagc agcgcaaggt cgaggagatc cgccgcttca
     3961 tcgacaggat ctacaaccat gtcggcaaga gcggacgcaa ggaagatatc aagtcgttta
     4021 ctgatgacga ggttatcgaa ctcgcgcgca acctgcgtaa tggcgtaccg gtcgcgacgc
     4081 cggtattcga cggcgccgcg gaggaagaga tcaaggaaat gctggtcatg gcgggcttgc
     4141 cgccttccgg ccagaccacg ctttacgacg gtcgcaccgg cgattccttc gagcgtccgg
     4201 tgacggtcgg ctacatgtac atcctcaagc tcaaccacct ggtggacgac aagatgcacg
     4261 cgcgttccac cggcccgtac tcgctggtca cgcagcagcc gctgggcggc aaggcccaat
     4321 tcggcggcca gcgcttcggc gaaatggaag tgtgggcgct cgaggcctat ggcgcgtcgt
     4381 acacgctgca ggaaatgctc acggtcaagt ccgacgacgt cacgggacgc accaagatgt
     4441 tcgagaacat cgtcaagggc gaccatcgca tggagccggg catgccggaa tccttcaacg
     4501 tactggtgaa ggagattcgc gccctcggca tcgacatgga gctggagcag gaaaaagagt
     4561 aggcccggtc gtcatcgtac cggaaccgga atttacatat atatagtcag cgcaacagtt
     4621 tgcaggagaa gataccttga aagacttgtt caatcttttc aagcagccag gcaagtcgga
     4681 agatttcgac gccattcgca tcgggcttgc ctccccggag aaaatccgct cgtggtccta
     4741 cggcgaggtc aagaagccgg agaccatcaa ttaccgcacg ttcaagccgg aacgtgatgg
     4801 cctgttctgc gccaagatct tcggaccggt caaggactac gaatgcctgt gcggcaagta
     4861 caagcgcctc aagcatcgcg gcgtgatctg cgagaagtgc ggcgtggaag tgacgctgtc
     4921 aaaggtgcgg cgcgagcgca tgggccacat cgacctggcc tcgccggtag cgcatatctg
     4981 gttcctgaag agcctgccct cccgcatggg cctgatgctg gacatgacgc tgcgcgacat
     5041 cgagcgcgtg ctgtatttcg aggcctacgt cgtgatcgat ccgggcatga cgccgctgga
     5101 gcgcgcctcg ctgctgtcgg aagacgccta tctcaacgcc atcgaacaat atggcgatga
     5161 gtttgacgcg cgcatgggcg ccgaggccgt gcgcgatctg ctgcgcgcca tcaatctgga
     5221 ggaagaagtc gccaagctgc gggccgaact ggccgacacc acctccgaga ccaagatcaa
     5281 gaagatcacc aagcgcctga aggtgctcga ggccttcctg tactccggca acaagcctga
     5341 gtggatggtg ctggaaatcc tgccggtgct gccgccggaa ctgcgtccgc tggtgccgct
     5401 cgacggcggc cgcttcgcga cctcggacct gaacgatctt tatcgccgcg tcatcaaccg
     5461 caacaaccgc ctgaagcggc tgcttgagct tggcgcgccg gacatcatca tccgcaacga
     5521 aaagcgca
//
LOCUS       DBNGZE010000439         3185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1310883, whole genome shotgun sequence.
ACCESSION   DBNGZE010000439 DBNGZE010000000
VERSION     DBNGZE010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1310883"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="AC16FV_11480"
     CDS             <1..884
                     /locus_tag="AC16FV_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2850705.1"
                     /translation="KLRQAISIAVDFEEQISIFENGRGIAAQGPLAPGIFGYREGQAG
                     INPYVYDWVDGKPRRKPLEYARRLLAEAGYPDGVDPASGKPLSINYETPAIGPERKAQ
                     LDWMLKQFSKLGIQLVVRATDYNRFQEKMRKGDAQIYDWGLNADYPDPENFLFLLYGP
                     NKKVGHDGENASNYDNPEFNRLFESMKNMENTPERARIIDQMLDIARRDSPWIWGMHP
                     KNFLLEHAWMSNTKLHNMANNTLKYSRIDPEKRAELRLAWNRPVTWPIVAILGVLAAS
                     AVPAVVAYRKRERGVAT"
     gene            994..1971
                     /locus_tag="AC16FV_11485"
     CDS             994..1971
                     /locus_tag="AC16FV_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2850706.1"
                     /translation="MIGYIIRRLLYAIPILIGVNLLTFALFFFVNSPDDMARVHLGGK
                     RVTPEAIVKWKADHGYDIPKFYNADRQGARKFTDTIFYKHSVKLFVFDFGRSDQGRDI
                     AYDISQRMWPSLAIALPTFLVGLLVYITFAMLIAFFRGTYLDYWGVVVCVVLMSVSTL
                     FYVIGGQFLVSKLWHLVPISGYDIGMESFKFLILPVIVGVVSGIGASTRLYRTFFLEE
                     INKDYVRTARAKGLSELRVLFRHVLKNAMIPILTGVVVVIPLLFTGSLILESFFGIPG
                     LGSYTIDAIGSQDFAIVRAMVFLGSVLYIVGLMLTDLSYTLVDPRVRLK"
     gene            1972..>3185
                     /locus_tag="AC16FV_11490"
     CDS             1972..>3185
                     /locus_tag="AC16FV_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2850707.1"
                     /translation="MNMSFQPVFLWTDVLIYVLLAALIAFGFYTARHEHLRVPWRRVA
                     RRPMAMAALVVLLAYVAVGLMDSIHYKPRLSGGADSETVYANEVLSLLDSVARPLRNR
                     MEKTYSAPFAVQAYAMESVEHPDGRLVREYPRLFYGGAHLQEPGERAGDILSRAGTAT
                     LAGFGIWSLLVTGFLLFWRGRNESWARTLRRLLQGEGDFPWRTALVTEGDLVVLTANA
                     AYLSLYYHIFGTDQVGQDVFYKSLKSVRTGLVIGTLTTLVMLPFAALFGIMAGYFRGW
                     VDDVIQYVYTTLSSIPSVLLIAAAILTLQAYIATHPESFTSATERADTRLLFLCLILG
                     VTSWTSLCRLLRGETLKLREVDYIQAARALGASHLSVMARHILPNVMHIVLITVVLDF
                     SGLVLAEAVLSYV"
BASE COUNT          584 a          941 c          958 g          702 t
ORIGIN      
        1 acaaactgcg tcaggcgatt tctatcgccg ttgatttcga ggaacagatt tccatcttcg
       61 agaacggccg cggcatcgcg gcgcagggcc cgctggcgcc ggggattttc ggctaccgcg
      121 aagggcaggc cggcatcaat ccatacgtat atgactgggt ggatggcaaa ccccggcgca
      181 agcctcttga atacgcgcgc cggctactgg cggaggcggg ttatcccgac ggggtggatc
      241 ccgcatcggg caaaccgctc tcgatcaatt atgaaacccc ggccatcggg ccggagagaa
      301 aggctcagct cgactggatg ctcaagcagt tcagcaagct cggcattcag ttggtggtgc
      361 gcgccaccga ttacaaccgc tttcaggaaa agatgcgcaa gggcgacgca cagatctacg
      421 actggggctt gaacgcggat tatcctgacc cggaaaattt cctgttcctg ctgtacgggc
      481 cgaacaagaa agtcgggcac gatggcgaga atgcctcgaa ctacgacaac ccggagttca
      541 accgcctgtt cgagagcatg aagaacatgg agaacacgcc cgaacgggcg cggatcatcg
      601 atcagatgct cgacatcgcg cggcgcgatt ctccgtggat ctggggcatg caccccaaga
      661 atttcctgct ggagcatgcg tggatgtcca acaccaagct gcacaacatg gccaacaaca
      721 ccctcaaata cagccggatc gacccggaaa aacgcgcgga gctgcgcctg gcctggaacc
      781 gccccgtgac ctggcccatc gtggcaatac tgggagtgtt ggcggcgagc gcggtacccg
      841 ccgtcgtcgc ttaccgtaaa agagaaagag gagtagcgac atgagccgtg gagcaggggc
      901 cccaagcaaa gcttggggat gcgaccggcg aattggcgca ggccgccgac aaagcggggc
      961 aacgattttg catggccttt cggaggcggc gtcgtgatcg gatacatcat ccgccgtctg
     1021 ttgtacgcca ttccgatcct gatcggggtg aaccttctca ccttcgcgct gtttttcttc
     1081 gtcaacagcc cggatgacat ggcgcgcgtg catctgggcg gcaagcgcgt cacgcccgag
     1141 gccatcgtca agtggaaagc ggatcacggt tacgacatac caaagttcta taacgccgac
     1201 aggcagggtg cgcggaaatt cactgacaca atcttttaca agcattcagt caagttgttt
     1261 gtgttcgact tcggccgctc cgaccagggg cgcgacatcg cctacgatat ttcccagcgc
     1321 atgtggccaa gcctcgcgat tgcgctgcca accttcctgg tgggattgct cgtatacatc
     1381 accttcgcga tgctgatcgc cttcttccgc ggcacttatc tcgactattg gggcgtggtc
     1441 gtctgcgtgg tgctgatgtc ggtctcaacg ctgttctacg tcatcggcgg ccagtttttg
     1501 gttagcaagc tctggcatct ggtgccgatc tcgggttacg acatcggcat ggagtcgttc
     1561 aaattcctga tcctccccgt cattgtcggt gtagtgagcg gaatcggcgc gagcacgcgg
     1621 ttgtatcgca cttttttcct ggaggaaatc aacaaggact atgtgcgtac cgcgcgcgcc
     1681 aaaggactgt ccgagctgcg cgtgctgttc cgtcatgtgc tcaagaacgc catgatcccg
     1741 attctcaccg gtgtcgtggt ggttatcccg ctgcttttca ccggcagcct gatcctggag
     1801 tcatttttcg gcatccccgg gctggggagc tataccatcg atgccatcgg ctcacaggat
     1861 tttgccattg tgcgcgccat ggtgtttctg ggttccgtgc tctacatcgt cgggctgatg
     1921 ctcaccgact tgagctatac cctggtggac ccgcgcgtgc gactgaaata aatgaacatg
     1981 tcattccaac ctgtatttct ctggactgac gtattgatct acgtgctgct cgcggcgttg
     2041 atcgccttcg gtttttatac cgcgcgtcac gagcacctgc gcgtgccctg gcgccgggta
     2101 gcgcggcgtc ccatggccat ggcggcgctg gtggtgctgc tggcgtatgt tgccgtgggc
     2161 ttgatggatt cgattcatta caaaccccgc ctgtccggcg gtgcggattc cgagactgtc
     2221 tacgccaatg aagtattgag cctgctcgat tccgttgcca ggccattgcg caatcgcatg
     2281 gagaaaacct attccgcgcc gtttgcggtg caggcgtacg ccatggaatc cgtggaacac
     2341 ccggacggcc ggcttgtgcg cgagtatccg cgcctgtttt acggcggcgc gcatttgcag
     2401 gaaccaggcg aacgcgccgg agatatcctg tcgcgcgccg gtactgctac gttggccggg
     2461 tttgggatct ggtcgttgct ggtgacgggc ttcctgctgt tctggcgcgg gcgcaacgag
     2521 tcctgggcgc ggacgctgcg ccggctgctg caaggggagg gcgatttccc ctggcgcacc
     2581 gcactggtta ctgagggtga tctggttgtt ctgacggcga atgccgctta cctgtcactt
     2641 tattatcaca tcttcggcac cgaccaggtc gggcaggacg tgttttacaa atccctgaag
     2701 agcgtccgca ccgggctggt gatcggcacg ctcacgacgc tggtcatgtt gccattcgcc
     2761 gcgttgttcg gcatcatggc cgggtatttt cgcggctggg tggatgacgt gattcagtac
     2821 gtctatacca ctctgagctc cattcccagc gtgctcctta tcgccgccgc catcctgact
     2881 ctgcaagcct atatcgcgac ccatcccgaa tcgttcacca gcgccaccga gcgcgcggac
     2941 acgcggctct tgttcctgtg cctgatactc ggcgtcacca gctggaccag tctgtgtcgc
     3001 ctgctgcgcg gcgagacgct caagctgcgc gaggtggact acatccaggc tgcgcgcgcc
     3061 ctcggcgcga gccatctttc cgtcatggca cgccatatcc tgcccaatgt catgcatatc
     3121 gttctcatca cggtggtgct ggatttcagc gggctggtgc tggccgaggc ggtgctgtcc
     3181 tacgt
//
LOCUS       DBNGZE010000440         4981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1241698, whole genome shotgun sequence.
ACCESSION   DBNGZE010000440 DBNGZE010000000
VERSION     DBNGZE010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1241698"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="AC16FV_11495"
     CDS             complement(<1..580)
                     /locus_tag="AC16FV_11495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2850708.1"
                     /translation="MSKKAEKTTKAGKSAKNGPVEAAAPVEQASVTTAASEPAVTTPE
                     KTEKKYILRATGLQKVFNEGTFNVHVLKGVYLRVEPGERVAIVGASGSGKSTLLHLIG
                     GLDRPTAGQVEIEGQDLTKMNETDVSVLRNRMLGFVYQFHHLLNEFTALENVAMPLLV
                     RRMPTQEAIAQAQAMLKRVGLEDRLHHRPGELS"
     gene            complement(577..1821)
                     /locus_tag="AC16FV_11500"
     CDS             complement(577..1821)
                     /locus_tag="AC16FV_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR2850709.1"
                     /translation="MIRPFELFVGLRYTRARKRSHFISIISLISILGITLGITALITT
                     LSVMNGFGKELRGRILGVISHVTITESTGQLHDWKGTAERIRNPHVTGRAPYIAGQGM
                     VARGKTVSGVMVRGILPSEEMQVSEFGSRMIEGKLDALKPGEFGVVIGSALAWKLDLT
                     VGSPVSLVIPQALVTPAGLMPRFKRFTVVGIFRMDMYEYDSGLVLMNLDDAAKLYQMG
                     DQVTGLRLKLDNLDAAPVVADELTAGLGPNYLARDWTREHGNFFRALRIEKTVMFVIL
                     LLIVTVAMFNVISTLVVVVTEKRADIAILRTLGASPKSIMGIFLAHGSVVGVVGTLVG
                     TISGVLLALNVEPIVHGIERLFSTSFIAADVYFISELPSDLHWSDVLLVSGSSLVLGL
                     LSALYPAWRASKVQPAEALRYE"
     gene            complement(1869..3020)
                     /locus_tag="AC16FV_11505"
     CDS             complement(1869..3020)
                     /locus_tag="AC16FV_11505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR2850710.1"
                     /translation="MVLIRHAGGPWRRLLVAALCALSVACATPPAPRSEPAPEAAPKA
                     APKPESKPAPVAVPAARPAELRIAAVGDIMLGGTAAPEMRKYGYDYPFELTRDILKQA
                     QIVFGNLEGPLTDGGAAGTAKQYVFCSPPDKVAPALARAGFNVVSLANNHSLDYGPEG
                     LEDTRAALDKAGIRLAGAGRNAAEARQPVYMMADGTTVAFLAYSLTFPEEFWAGPDKP
                     GTAFGHEKHVRADVAAARQTADIVVVSFHWGQEGKTELRDYQTQLAHAAIDAGASAVL
                     GHHPHILQGVERYKDGVILYSLGNFAFGSFSNTATRSAVALLSFRDKRWRELRMVPLN
                     VKNAEVVFQPRPLVGRDAAEVVEHLQQLSQSLGTTLENRDGNAVLTNND"
     gene            complement(3036..3512)
                     /locus_tag="AC16FV_11510"
     CDS             complement(3036..3512)
                     /locus_tag="AC16FV_11510"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR2850711.1"
                     /translation="MTRKGPRNAGAHLPTEILKRAGVHMDQLSGLLSHWKASVGEPLV
                     SHSFPVSYANGRLSLRADTSAWASRLRHSQAEIMDELRHDPYFHDLHELYVRVLPERA
                     RAPGEKKRQSIVLPSRIPDSAANLIKSVADDILDPALRRSLIRLAGARDKSAPAKR"
     gene            3631..4347
                     /locus_tag="AC16FV_11515"
     CDS             3631..4347
                     /locus_tag="AC16FV_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR2850712.1"
                     /translation="MNQNFDAIASNLLRVRQSIVDAARVCTRSSDAIRLIAVSKGHPN
                     EAIAAAIAAGQKDFGESTTQDALPKISRLANPSIDWHFIGHLQTNKAKFVPGNFSWLH
                     SLDNLDLARKLFRLAGEKSATINILIEVNVTRDPKKHGIAPDALPNFVEKLLKENLTA
                     LPLRGLMTIAPYEAPEKDIRACFASLRRLRDDCQQRFGLKNFTELSMGMSSDYIEAIK
                     EGATMVRVGTAIFGERDYSK"
     gene            4441..4905
                     /locus_tag="AC16FV_11520"
     CDS             4441..4905
                     /locus_tag="AC16FV_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2850713.1"
                     /translation="MKNVKMRSLLATGSILIMLAPLALRAAEPEDVIKYRQNVMKAIG
                     GHSSAAGAIVQGKVDYKSDLLEHARSMQALTRNIPTLFPKDSDFGETKAKDEVWSKRA
                     DFEKRAKDTKEKIEVFAKAVQSSNQQTIAASFKDVGESCKACHKDFRKKEEE"
BASE COUNT         1006 a         1544 c         1495 g          936 t
ORIGIN      
        1 cggagagctc gcccggccgg tggtgcaagc gatcttcgag gccgacgcgc ttgagcatgg
       61 cctgcgcctg cgcgatcgcc tcttgcgtcg gcatgcgccg caccaacagc ggcatggcga
      121 cattctccag cgccgtaaac tcgttcagca aatgatggaa ctgatagaca aagccgagca
      181 tgcgattgcg caacacgctg acgtccgtct cgttcatttt cgtcaagtcc tggccctcga
      241 tttccacctg cccggccgtg ggccggtcca gcccgccgat caggtgcaac aatgtgctct
      301 tgcccgagcc ggaggcaccg acgatggcga cgcgctcgcc cggttccacg cgcaggtaca
      361 cgcccttcag cacgtgcacg ttgaacgtgc cctcgttgaa caccttctgc aggccggtgg
      421 cgcgcagaat atattttttc tcggttttct ccggcgttgt cacggccggc tccgacgccg
      481 ccgtcgtcac actcgcctgt tccaccggag cggcggcctc gaccgggccg ttcttcgcgg
      541 acttgccggc cttcgtggtt ttttcagcct tcttgctcat tcgtaacgca aggcctcagc
      601 cggctgcacc ttggaggcgc gccaggcggg atagagggcg gacagcaggc ccagcaccag
      661 cgaactgccc gataccagca acacgtcgga ccagtgcagg tcggacggca gctcgctgat
      721 gaaatacaca tccgcggcga tgaaagaagt gctgaacaga cgttcgatgc cgtgcacgat
      781 gggctcgacg ttgagcgcca gcagcacgcc gctgatcgtg cccaccagcg tgccgacgac
      841 gccgaccacc gagccgtgcg cgaggaatat gcccataata cttttggggc tggcgccgag
      901 cgtgcgcaaa atggcgatgt cggcgcgttt ctcggtgacc acgaccacca gcgtggagat
      961 gacgttgaac atggccacgg tcacaatcag cagcagaatg acgaacatga cggtcttttc
     1021 gatcctgagc gcgcggaaaa agttgccatg ctcgcgcgtc cagtcgcgcg ccagataatt
     1081 cggccccaat ccggctgtca gttcgtccgc caccaccggc gccgcatcga gattgtcgag
     1141 cttcaggcgc agcccggtga cctgatcgcc catctggtat agcttcgcgg cgtcgtccag
     1201 gttcatcagc accaggccgc tgtcgtattc gtacatgtcc atcctgaaga tgccgaccac
     1261 ggtgaagcgc ttgaaacgcg gcatgaggcc ggccggcgtc accagcgcct gcggaatcac
     1321 caacgacacc ggcgagccca cggtgagatc gagcttccac gccagcgcgc tgccgatcac
     1381 gacgccgaac tcgcccggct tcagcgcgtc cagcttgcct tcgatcatgc ggctgccgaa
     1441 ttcggatacc tgcatctcct cggacggcaa tatcccacgc accatcacgc cgctcacggt
     1501 cttgccgcgt gccaccatgc cctgcccggc gatatagggt gcgcgcccgg tcacgtgcgg
     1561 atttcttatg cgctcggccg tgcccttcca gtcgtgcaac tggccggtgg actcggtgat
     1621 ggtcacatgc gagatcacgc cgaggatgcg gccgcgcagt tccttgccga agccgttcat
     1681 caccgacaac gtcgtgatga gcgcggtgat accgagcgtg atgccgagaa tggaaataag
     1741 cgagatgatg gagatgaagt ggctgcgttt gcgggcacgg gtgtagcgca gaccgacgaa
     1801 aagctcaaac ggacggatca tggtccggca ttaaacacag atgcaggaat tcgcacaagt
     1861 gccttgattc agtcgttgtt cgtcagcacg gcgttgccgt cgcggttttc cagcgtggtg
     1921 ccgagcgatt gtgacaactg ctgcaaatgc tcgacgacct ccgcggcatc gcgcccgacc
     1981 agcggacgcg gctggaacac gacctcggca ttcttcacgt tgagcgggac catgcgcagc
     2041 tcgcgccagc gtttgtcccg gaacgacagc agcgcgaccg cgctgcgcgt ggcggtgttg
     2101 ctgaacgacc cgaaggcgaa gttgccgagg ctgtaaagga tcacgccgtc cttgtagcgt
     2161 tccaccccct gcaggatgtg cggatgatgg cccagcaccg cggacgcgcc ggcgtcgatg
     2221 gcggcgtgcg ccagttgcgt ctgatagtcg cgcaactcag tcttgccctc ctgcccccag
     2281 tggaacgaga ccaccacgat atcggccgtc tgacgtgccg ccgccacgtc ggcgcgcaca
     2341 tgcttctcat ggccgaaggc ggtcccgggc ttgtccggcc cggcccagaa ttcctccgga
     2401 aacgtcaggg aataggccag aaacgcgacg gtcgtcccat ccgccatcat atataccggt
     2461 tggcgtgcct cggcggcgtt tcgtcctgcc ccggcaagac gtatcccggc cttgtccaac
     2521 gccgcacggg tatcttccag gccctcgggg ccgtaatcca gcgagtgatt gttggcgagc
     2581 gagacgacat taaagccggc gcgcgccagt gccggggcca ccttgtccgg cggcgagcag
     2641 aaaacatatt gcttggcagt cccggccgcg ccgccatcgg tcagcgggcc ttccagattg
     2701 ccaaacacga tctgtgcctg cttcaggatg tcccgggtca gctcgaaggg atagtcgtag
     2761 ccatatttgc gcatctccgg cgccgccgtg ccgccgagca tgatgtcgcc aaccgcggcg
     2821 atgcgcagtt ccgccgggcg tgccgcgggc acagccacgg gggcaggttt cgattcaggc
     2881 ttgggcgccg ccttcggagc cgcttccggg gcaggctcgg aacgcggtgc gggaggtgtg
     2941 gcgcaggcga cgctcagggc gcaaagcgcc gccacgagca gacgccgcca cggcccgccg
     3001 gcatgtcgga tcaggaccat gaactgatta taggattatc tttttgcggg tgccgacttg
     3061 tcgcgcgcgc ccgccaggcg gatcagcgag cggcgtagcg ccgggtcgag gatatcgtcc
     3121 gccacgctct tgatgaggtt ggcggcgctg tccggtatcc ggctgggcaa cacgatcgac
     3181 tggcgcttct tctcgcccgg cgcacgcgcc cgttcgggca acacgcgcac gtacagctcg
     3241 tgcaggtcgt ggaaataggg atcatggcgc agttcgtcca tgatctccgc ctggctgtgg
     3301 cgcaggcgac tggcccaggc cgaggtatcg gcgcgcaaac tgaggcggcc gttggcgtaa
     3361 ctgacgggaa aactgtgcga cacgagcggc tcgccgacgc tcgccttcca gtgcgacagc
     3421 aggccggaaa gctggtccat gtgcacaccc gcccgtttca atatctcggt gggtaaatga
     3481 gcgccggcat tccggggtcc tttccttgtc atgggcttct cacctcttgt gtacgttcca
     3541 tccaacgcgg ccggtcatta tgctcaaata aaaattgcac gcaacgttga caagtgaaaa
     3601 tttttttcaa atcacggtaa catgcaaaat gtgaaccaaa attttgacgc gatcgcaagc
     3661 aatttattgc gtgtacgaca gtccatcgtt gacgccgcgc gcgtgtgcac gagaagttcc
     3721 gacgctatcc gcttgattgc ggtgtcaaaa ggtcatccaa atgaagcgat agcggccgct
     3781 atcgccgccg gacaaaaaga tttcggtgaa agtacaacac aagacgccct cccgaagatt
     3841 tcacgacttg caaatccatc catcgactgg catttcatcg gccatctcca gaccaacaag
     3901 gccaaattcg ttccgggaaa tttttcctgg ctgcactcgc tcgacaacct cgacctcgcg
     3961 cgtaaacttt tccgactcgc cggggaaaaa tcagcaacca ttaatatact gatagaggtc
     4021 aacgtcaccc gcgatccgaa aaaacacggc atcgcccccg acgccctgcc gaatttcgtc
     4081 gaaaaattat taaaggagaa cctcacagcc ctgcccctgc gcggtctcat gaccatcgcc
     4141 ccctatgagg ctccggaaaa ggacattcgt gcctgcttcg cgtccctgcg gcggctacgc
     4201 gatgactgtc agcagcgttt cggactgaaa aatttcaccg aactgtccat gggcatgagc
     4261 agcgactata tcgaagccat aaaagaaggc gcaaccatgg tgcgagtcgg gacggcgata
     4321 ttcggggagc gggattattc gaaataatcg tcgaactgcc gacgtcattt cttgtcagaa
     4381 ttcgcgtttg aatttgcggg taatcagaat ttttcggaac atccaggcaa ggagcacatc
     4441 atgaaaaatg taaaaatgcg cagtctgctg gcgacgggct cgatattgat catgctggcg
     4501 ccgctggcgc tgcgtgcggc ggagccggag gacgtcatca aatatcgtca gaacgtcatg
     4561 aaggccatcg gcggtcacag ctccgcggcc ggggccatcg tgcagggcaa ggtggattac
     4621 aagtccgatc tcctggaaca cgcgcgttcc atgcaggcat tgacccgcaa cattccgacg
     4681 ctgttcccca aggattccga ttttggcgag accaaggcca aggacgaggt ctggtcgaag
     4741 cgggcggatt tcgagaaacg cgcgaaggac accaaggaaa agatcgaggt cttcgccaag
     4801 gcggttcaga gcagcaatca acagaccatc gccgccagct tcaaggacgt gggtgaaagc
     4861 tgcaaggcct gccacaagga ttttcgcaag aaggaagagg aataaaccaa ttccctcgcc
     4921 ccaggatgcc ttgtgaccat cagcggcgtg gcgttgttgc ttgggttgat tgtcgcggcg
     4981 g
//
LOCUS       DBNGZE010000441         8731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1311134, whole genome shotgun sequence.
ACCESSION   DBNGZE010000441 DBNGZE010000000
VERSION     DBNGZE010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1311134"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC16FV_11525"
     CDS             <1..530
                     /locus_tag="AC16FV_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2850714.1"
                     /translation="LACLLMQISHGPYYTFYSIYLEGYGYSKAVIGMLWAFAVVCEIG
                     VFLLMQHLLTRAPLRAVLIASFFLAAIRWLLIGHYPENFSVLVIAQALHAATFGSFHA
                     TAMQLVHRFFTGKHQHRGQAIYGSLSFGIGGAVGSLYSGHTWATLGPTVTFNIAAGAA
                     GLAFLVAWLFIKEKL"
     gene            complement(652..1071)
                     /locus_tag="AC16FV_11530"
     CDS             complement(652..1071)
                     /locus_tag="AC16FV_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850715.1"
                     /translation="MNFKELLEQLEEHLGFHQFPVNPAATNIKDVFDGSPLHHDLMTR
                     FVRAIYVSNACRRLTDPVKLDKTFHALTPIRQEILRAARTDVDLYRVLDELGAALNQI
                     FAESLVAKKMAGPVVRSTAEIIPLSAYRHLRRLKSSA"
     gene            complement(1068..1937)
                     /locus_tag="AC16FV_11535"
     CDS             complement(1068..1937)
                     /locus_tag="AC16FV_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR2850716.1"
                     /translation="MDISALQAFLAVAESGSFSRAAERIYLTQPAISKRIAALEKEIG
                     ARLFDRIGRGIHLTPAGEALLTRARNVLKELEDVKRGITNLSGSISGELLLATSHHIG
                     LHRLPGLLKRFHETYTQVRLNLQFMDSEKACQAVARGNLELAVVTLPPEAPAPLKVEK
                     IWDDPLDIVVSPGHPLAREKRVKLAKLLEYPAILPGPGTYTREIILNAFGPLRDKIQV
                     GMATNYLEVLKMLAAIGLGWSALPRTMIDEGLNVVQIEKVEIRRELGIVTHEKRTLSN
                     AGQAMIQIIRESK"
     gene            2031..3437
                     /gene="leuC"
                     /locus_tag="AC16FV_11540"
     CDS             2031..3437
                     /gene="leuC"
                     /locus_tag="AC16FV_11540"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR2850717.1"
                     /translation="MVGKTLYDKLWDAHVVRHNDDGTALLYIDRHLVHEVTSPQAFEG
                     LKLAGRKVWRIDANLATADHNVPTTDRARGIADPVSRLQVETLDQNCHDYGITEFGMH
                     DARQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLI
                     LKKAKNMRVRVEGKLPAGVTAKDIVLAIIGKIGTAGGTGHAIEFAGEAIGQLSVEGRM
                     TVCNMAIEAGARAGMIAVDDKTIEYFRGRPYAPKGAQWDMAVKAWGELHSDPDARFDK
                     EVVLNAADIKPQVTWGTSPEMVVPVDGAVPDPEKESDSVKRGSMQQALKYMDLKPGTP
                     MRAIALDKVFIGSCTNSRIEDLRAAAAVVNGKKVARNIKLAMVVPGSGLVKAQAEKEG
                     LDKIFRDAGFEWRAPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPA
                     MAAAAAIAGHFVDVREFH"
     gene            3437..4078
                     /gene="leuD"
                     /locus_tag="AC16FV_11545"
     CDS             3437..4078
                     /gene="leuD"
                     /locus_tag="AC16FV_11545"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR2850718.1"
                     /translation="MQKFEKLTGLAMPLDRANVDTDAIIPKQYLKSIKRTGFGPTLFD
                     DWRYLDPGEPGMDHGKRRINPDFVLNQSRYQGAQVLLARDNFGCGSSREHAPWALMDY
                     GFRAIIAPSYADIFYNNSFKNGLLPIVLDRAAVDQLFRETDATPGYRLTIDLAAQTVV
                     TPTGKQFKFEIDAFRKHCLLNGLDDIGLTLQHVDEIKAYEARRRNEAPWLFAK"
     gene            4090..5169
                     /gene="leuB"
                     /locus_tag="AC16FV_11550"
     CDS             4090..5169
                     /gene="leuB"
                     /locus_tag="AC16FV_11550"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR2850719.1"
                     /translation="MTKKIAVLPGDGIGPEVVAEAVKVLERLRRDFGLKIEMETAQVG
                     GTAYDAHKHPLPDATLKLAKQADAVLLGAVGGTQWDTLPRELRPEKALLGLRAELKLF
                     ANLRPAILYPQLASASTLKPEIVAGLDLLIVRELTGDIYFGEPRGVRPLANGERQGFN
                     TMVYAEHEVERVSRVAFEAARKRRKKLCSVEKANVLETSGLWREVVIRVAKDYPDIEL
                     SHMYVDNAAMQLVRAPKQFDVIVTGNIFGDILSDEAAMLTGSIGMLPSASLDSANKGM
                     YEPIHGSAPDIAGKGVANPLATILSAAMMLRYTLNEPQLADKIEQAVSRVLDQGLRTG
                     DIHTEGTKKVGTKAMGDAVVQALGA"
     gene            5284..6399
                     /gene="asd"
                     /locus_tag="AC16FV_11555"
     CDS             5284..6399
                     /gene="asd"
                     /locus_tag="AC16FV_11555"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR2850720.1"
                     /translation="MKRVGLVGWRGMVGSVLMGRMQAEKDFDHIEPVFFTTSNVGGKG
                     PAIGKDVPPLKDAKNINELKAMDVIITCQGGEYTSEIFPKLRAAGWKGYWIDAASSLR
                     MEKDAIIILDPVNLDVIKAGLKNGVLNYIGGNCTVSLMLMALGGLFKANLIEWMTSMT
                     YQAASGAGANNMRELVKQMGSIHAAVKDKLDDPASAILDIDRAVADHIRGDKYPKEFF
                     GVPLAGSIIPYIDKQLDSGQSKEEWKGQAETNKILGRSEQPIPIDGICVRVGAMRCHS
                     QALTIKLKRDVPMDEINAMLAAHNSWVKVVPNERDKSMRELTPAAVTGTLTVPVGRLR
                     KLNMGKDYLGAFTVGDQLLWGAAEPLRRMLRILLESR"
     gene            6744..>8731
                     /locus_tag="AC16FV_11560"
     CDS             6744..>8731
                     /locus_tag="AC16FV_11560"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0044096 - type IV pilus [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein FimV"
                     /protein_id="HIR2850721.1"
                     /translation="MFRNRRGIALVSQWMLVGIMALFPAISHALALGKLKVISALNEP
                     LNAEIELTSITEKELKGLSVSLASRPDFDAAGVERLPFLSELKFVVDRKSDGRHFLKL
                     HTEQQVDEPFLHFLLQVEWAGGRLVREYTALIDPPYKIAGKAAPVETPVVTPPAPEPV
                     PAPIPEAQPAPPAPVAEAPQAEIPKEEVPPVVAKPEEPKAAEEPKAEAVPPQEEVAKE
                     APPPETVPAEQKLGPPEIAQGAAPEEPAAPKVEPLEAAPKTEEAPATAAQPPVEPEAK
                     PAPVLAQMTDYSVKRGDTLWRIAEKARADNRDVSIEQMILAIYRTNKNAFFGNNVNNL
                     KAGKILKMPEHEEVSSVTSSQARREFRAQYDAWQEYKLKLASASGAIQVPEAPEAAPE
                     AAAPAPTPVPGPKKEAAKPSKPAPSATDRAKQDELLKIVRNTLQQEKSTPDRKVAEKE
                     SAREAAAREQQALADRAATLEESLESKRLENKELSDKVGLVRSQLKKESRLIELENQS
                     LAQQPKPAETKPAEPAVKPAEPAPKPVEKATAPVPEQQAKIAALPPAKPAPAAPKKKA
                     SLPPPPEEKDFLATVLEAVQSDLLVPVVIGVVVLLSGGIALVYFRRRQKSIAEFEESI
                     LSADAISTEQPATTGTVVGQQPVAAATTGGDTSFLSDF"
BASE COUNT         1876 a         2510 c         2696 g         1649 t
ORIGIN      
        1 tgctggcctg cctgctgatg cagatcagtc acgggccgta ctacaccttt tattccatat
       61 atctcgaagg ctacggttac tcgaaagccg tgatcgggat gttgtgggcc ttcgccgtgg
      121 tgtgcgagat cggtgtgttt ctgttgatgc agcacctgtt gacgcgtgcg ccactgcgcg
      181 cagtattaat agcgagcttc ttcctcgcgg caatacgctg gctgctcatc ggccattatc
      241 cggaaaactt ttccgtatta gtgatcgcgc aggcgttaca tgctgcgact ttcggcagct
      301 ttcacgccac ggccatgcaa ctggtgcacc ggtttttcac gggcaaacat cagcatcgcg
      361 gacaggcgat atacgggagt ctcagcttcg ggatcggcgg ggcagtgggg agtttgtaca
      421 gcggtcacac ctgggccacc ttgggcccga ccgtgacttt caacattgcg gccggagccg
      481 cggggctggc gtttttggtg gcgtggctat ttattaaaga aaaactttaa tcaattctaa
      541 tctccgttca tggtgagctt gtcgaaccat gagcggaggg ccgttcggtc ttcgacaagc
      601 tcagaccgaa cggcgagtcg aagggctcag cacgagcgga attagaaatt tttaagccga
      661 agacttgagc cggcgcaggt ggcgataggc ggagagtggg atgatttcgg cggtgcttcg
      721 taccacgggc ccggccatct ttttggcaac cagactttcc gcgaagatct ggttcagcgc
      781 ggcaccgagc tcgtcgagca cgcgataaag atcgacgtca gtgcgcgcgg cgcgcaggat
      841 ttcctggcgg atcggcgtga gggcgtgaaa ggtcttgtcc agttttaccg gatcggtcag
      901 gcggcggcag gcgttggaga cataaatcgc gcgcacgaaa cgcgtcatca ggtcgtggtg
      961 cagcgggctg ccgtcgaaca cgtccttgat gttggtcgca gccggattca ccggaaattg
     1021 gtgaaagccc agatgttctt ccaactgctc cagcaattct ttgaagttca ttttgattct
     1081 ctgattattt gtatcatcgc ctgaccggcg tttgagagag tgcgcttctc gtgggtgaca
     1141 atgcccagct cacgccgtat ctccaccttt tcaatctgga ccacgttcag cccttcgtca
     1201 atcatagtcc gtggaagtgc gctccacccg agaccgatgg cggcgagcat tttgagcact
     1261 tccagatagt tggtagccat gccgacttga attttgtcgc gcaacggtcc gaaggcattg
     1321 aggatgattt cacgcgtata agtgcccgga ccgggcagaa tcgccggata ttccagcagc
     1381 ttcgcaagct ttacgcgctt ttcgcgcgcc agcgggtgcc cgggactgac gacgatatcg
     1441 agcggatcgt cccagatttt ctcgactttc aggggcgctg gggcttcggg cggcagggtg
     1501 accaccgcca gctccagatt gccgcgggcc acggcctggc aggccttttc cgaatccatg
     1561 aactgcagat tcaagcgcac ctgtgtgtag gtttcgtgaa aacgcttgag cagccccggc
     1621 agccggtgca ggccgatgtg gtggctggtc gccagcaaaa gctcgccgga aatgctccct
     1681 gaaaggttgg taatgccacg tttgacgtcc tccagctcct tcagcacgtt gcgcgcgcgg
     1741 gtcagcaacg cctcgccggc aggcgtcagg tgaataccgc gcccgatgcg gtcgaacagg
     1801 cgcgcgccta tctctttttc gagcgcggcg atgcgcttgc tgatggcggg ctgggtcagg
     1861 tagatgcgct ccgcggcgcg ggaaaatgac ccggattcgg cgacggcgag gaaggcttgc
     1921 agggcggata tatccataac ttaatagaat atattacatg ataaacatga atttgtgtta
     1981 ttcatgccgc atgcctagaa tttgcacaat tcttcactct gttggcagcc atggtcggga
     2041 aaaccctcta cgacaaactc tgggatgcgc atgtggtgcg tcacaatgat gacggcaccg
     2101 cgctccttta tatcgaccgc cacctggtgc atgaagtcac cagcccgcag gcgttcgagg
     2161 gcctgaagct cgccgggcgc aaggtctggc gcatcgacgc taatctcgcc accgccgatc
     2221 acaacgtccc gaccacggac cgcgcccgcg gcatcgccga cccggtgtcg cgcttgcagg
     2281 tggaaaccct cgatcagaac tgccacgact acggcatcac cgaattcggc atgcacgacg
     2341 cgcgtcaggg gatcgtgcac gtgatcggcc cggagcaggg cgcaaccctg cccggcatga
     2401 ccgtggtgtg cggcgattcg cacacctcga cccacggcgc cttcggcgcg ctcgctttcg
     2461 gcatcggcac ctccgaggtc gagcatgtgc tggccacgca atgtctgatt ttgaagaagg
     2521 cgaaaaacat gcgcgtgcgg gtggagggaa aacttcccgc aggcgttacc gccaaggaca
     2581 tcgtgctcgc gattattgga aaaattggga ccgctggtgg gaccggccac gccatcgagt
     2641 tcgccggcga ggccatcgga caactttccg tcgagggccg catgacggtg tgcaacatgg
     2701 ccatcgaggc cggcgcgcgc gcaggcatga tcgcggtgga cgacaagacc atcgaatatt
     2761 tccgtggccg gccgtacgcg cccaagggcg cgcaatggga tatggcggtg aaggcctggg
     2821 gtgaattgca cagcgatccg gatgccaggt tcgataaaga ggtcgtactc aatgccgccg
     2881 acatcaagcc gcaggtgacc tggggcacgt cgcccgagat ggtggtgccg gtggacggcg
     2941 ccgtgcctga tccggaaaag gagtccgatt ccgtcaaacg cggcagcatg caacaggcgc
     3001 tcaagtacat ggatctcaaa ccgggaactc cgatgcgcgc gattgcgctc gacaaggttt
     3061 tcatcggctc gtgtaccaac tcgcgcatcg aggatttgcg cgccgccgct gccgtggtca
     3121 acggaaaaaa agtggcgcgc aatatcaagc tcgcgatggt ggtgccgggt tccggcttgg
     3181 tgaaggcgca ggcggaaaag gaaggactcg ataaaatttt ccgtgatgcc ggtttcgaat
     3241 ggcgtgcccc gggatgctcc atgtgtctcg ccatgaacgc cgaccggctc gaaccgggcg
     3301 aacgttgcgc ctcgacatcg aaccgcaatt tcgagggacg ccagggcgct ggcggacgca
     3361 cgcatcttgt gagtcccgcc atggccgcag ccgcggcgat cgcagggcat tttgtggatg
     3421 tacgagaatt tcactgatgc agaaatttga aaaattgaca ggcttggcga tgccgctgga
     3481 tcgtgccaat gtggacacgg acgcgatcat cccgaagcaa tacctgaaat cgatcaagcg
     3541 tactggcttc ggaccgactc tgttcgatga ttggcgttac ctcgatccgg gcgagcccgg
     3601 catggatcac ggcaagcgcc gcatcaatcc ggatttcgta ttgaatcagt cgcgctatca
     3661 gggcgcgcag gttttgctgg cgcgcgacaa tttcggttgc ggttcctcgc gcgagcatgc
     3721 gccgtgggca ctgatggact acggtttccg cgccatcatc gcgccgagct atgccgatat
     3781 tttttacaac aacagcttca agaacggttt gttgccgatt gttcttgatc gcgccgccgt
     3841 ggatcagctg ttccgcgaaa ccgacgcgac tccgggttac cggttgacta tcgatcttgc
     3901 cgcgcagacc gtggtgacgc ctacgggcaa gcagttcaag tttgagatcg acgccttccg
     3961 taagcactgt ttgctgaacg gcctcgatga catcgggctt accttgcaac atgtggatga
     4021 aataaaggct tatgaagcac gacgccgcaa cgaagcgccg tggctcttcg cgaagtaaaa
     4081 ggacaaacga tgaccaaaaa gattgcggta ttgcccggtg acgggatcgg tcccgaagta
     4141 gtggccgagg cggtcaaggt tctcgagcgc ctgcgccggg atttcggcct caagatcgaa
     4201 atggagaccg cgcaggtcgg tggcacggcc tatgatgccc acaagcatcc gctcccggat
     4261 gcaacgctga aactggcgaa acaggcagac gccgtgctgc tcggggccgt gggcggcacc
     4321 cagtgggata cgctgccgcg cgagctgcgc ccggaaaagg ccctgctggg cctgcgcgcc
     4381 gagctcaaac tgttcgccaa cctgcgtccg gcgatactct atccgcagct ggcctcggcc
     4441 tcgacgctca agcccgagat cgttgcgggg ctcgacctgc tgatcgtgcg cgaactgact
     4501 ggcgatatct acttcggcga accgcgcgga gtgcgcccgc tggccaatgg cgagcgccag
     4561 ggattcaata ccatggtgta tgccgagcac gaagtcgagc gtgtgtcacg ggtggcgttc
     4621 gaggccgcgc gcaagcgtcg caaaaaactg tgctcggtgg aaaaagccaa cgtactggag
     4681 acctccggcc tgtggcgcga ggtggtgata cgggtcgcga aggattatcc ggatattgag
     4741 ttgagccata tgtacgtgga caacgcggcg atgcaactgg tgcgcgcgcc caagcagttc
     4801 gacgtcatcg ttaccggcaa tatcttcggt gatatcctgt cggacgaggc ggcgatgctg
     4861 acgggttcca tcggcatgct gccgtcggcc tcgctggaca gtgccaataa gggcatgtac
     4921 gagccgattc atggttcggc cccggatatt gccggcaagg gcgtggccaa tccgctcgcg
     4981 accatactct ccgcggcgat gatgctgcgt tacacgctca atgagccgca gctcgcggac
     5041 aaaatcgaac aggcagtttc ccgcgtgctc gatcagggtc tgcgcacggg cgacattcat
     5101 accgaaggaa caaagaaggt aggaacgaaa gcaatgggcg atgccgtcgt tcaggcgctt
     5161 ggcgcctgaa cgagtgttta gtttttagtg ttaagtgttt agttcatatt gcgcgcagaa
     5221 cgcactcctt aactcaacac taaacactta caacttaaaa ctttttgatg tgaggatata
     5281 gcgatgaagc gggtaggttt ggtaggttgg cgcggcatgg tgggctcggt attgatgggc
     5341 cgcatgcagg cggaaaaaga tttcgatcac atcgagccgg tgttctttac cacctcgaat
     5401 gtcggtggca agggccccgc gatcggcaaa gacgtcccgc cgctcaagga cgccaagaat
     5461 atcaacgaac tgaaggcgat ggatgtcatc atcacctgtc agggcggcga atacacttcc
     5521 gaaatattcc ccaaactgcg cgccgccgga tggaagggct actggatcga tgccgcctca
     5581 agcctgcgca tggaaaagga tgcgatcatc atcctcgatc cggtcaatct cgatgtcatc
     5641 aaggccggac tcaagaacgg cgtgctgaac tacatcggcg gcaactgcac ggtgtcgctc
     5701 atgctcatgg cgctcggcgg tctgttcaag gccaacctca tcgagtggat gacctcgatg
     5761 acctatcagg ccgcctccgg cgccggcgcc aacaacatgc gcgagctggt gaagcagatg
     5821 ggcagcattc acgcggccgt gaaggacaag ctcgacgacc cggcctcggc gattctggac
     5881 atcgaccgcg cggtggccga tcacatccgc ggcgataaat acccgaagga attcttcggc
     5941 gtgccgctgg ccggctcgat catcccttat atagacaaac agctcgacag cggacaatca
     6001 aaggaagagt ggaaaggtca ggccgagacc aacaagatcc ttgggcgctc ggaacagccg
     6061 atcccgatcg acggcatctg cgtgcgcgtc ggcgccatgc gctgccacag ccaggcgctc
     6121 accatcaagc tcaagcgcga cgtgccgatg gatgagatca atgccatgct ggccgcgcat
     6181 aattcctggg tgaaggtggt gccgaacgag cgtgacaaga gcatgcgtga acttaccccg
     6241 gccgcagtta cgggcacgct cacggtgccg gtgggacgtc tgcgcaaatt gaacatgggt
     6301 aaggattacc tgggtgcctt taccgttggc gaccagctac tgtggggcgc cgcggaaccg
     6361 ctgcggcgca tgttgcgcat cttgctggag agccggtaac tcccgaaaat ccccgttttg
     6421 cctgcctgaa acgcgagccc ggcggcatat tgatgtcggc cgggcgcgcg actatataaa
     6481 aaagaaagct cagaatgcgc caaatgcggc cggaacaaga tgcggcccgc atgtggtggc
     6541 catgccggcg ggctagcctc atttttacag ctaaattcag gggtgtaagc ccgttggccg
     6601 gtaacgggat gtctggtaaa aattcatctg tacgctatag ttagttgagg tttgtaaaga
     6661 gtattgctat ttatttgcct aactcccggt cttataaggc ctttggacaa ggaaacccgt
     6721 tgattacaag aggggtgtac aggatgtttc ggaacaggag gggaatagcg cttgtctcgc
     6781 aatggatgct ggtcggcatc atggcgctgt tcccggcaat cagtcatgca ctggcattgg
     6841 gcaagttgaa agtcatctcc gccctgaacg aaccgctcaa tgccgagatt gaactcacct
     6901 ccatcaccga aaaggaactg aaagggctca gcgtttcgct cgcgtcgcgc ccggacttcg
     6961 atgccgcggg tgtggagcgc cttccgtttc tgtctgaact caagttcgtg gtcgatcgga
     7021 aatccgacgg ccgtcatttc ctcaagcttc acaccgaaca acaggtcgac gaaccctttc
     7081 tgcattttct gttacaggtt gaatgggccg gcggtcgtct cgtgcgcgaa tacaccgcgc
     7141 tgatcgatcc accctacaaa atcgccggca aggccgctcc tgtcgagaca cccgtggtga
     7201 ctccgccggc gccggaaccc gtgccggcac cgataccgga ggcgcaaccg gcaccgccgg
     7261 cgcccgtggc tgaagcaccc caagcggaaa taccgaaaga agaagtcccg cccgtcgtcg
     7321 ccaaaccgga agaaccgaaa gcagccgagg aacctaaagc agaagccgta cctccccaag
     7381 aagaagtcgc caaggaagca ccgccaccgg agacggtgcc ggcagaacag aaacttggac
     7441 cgccggagat agctcagggc gcggcgccgg aagagcctgc tgcgccgaaa gttgaacccc
     7501 tggaagcggc ccccaagacg gaggaggcgc cggcaaccgc ggcgcaaccg cccgtggagc
     7561 ctgaagccaa accggctccg gtgctggcgc agatgacgga ttacagcgtc aagcgcggcg
     7621 acacgttgtg gcgtatcgcc gagaaggcgc gcgccgacaa tcgtgatgta agcatcgaac
     7681 agatgatcct cgcgatctat cgcaccaaca agaacgcctt tttcggcaat aacgtcaata
     7741 acctgaaggc gggcaaaatc ctcaagatgc ccgagcatga agaagtgagc tcggtcacgt
     7801 catcccaggc gcggcgtgaa ttccgcgcgc aatacgacgc gtggcaggaa tacaaattga
     7861 agctggcgag cgccagcggc gctatccagg tgccggaagc ccccgaagcg gcgccggaag
     7921 cagccgctcc tgcgccgacg cctgtaccgg ggccgaagaa agaagcggcg aaaccgtcga
     7981 agccggcgcc gtctgcgacg gacagagcca agcaggacga actgctcaag atcgtgcgca
     8041 acaccttgca gcaggagaag agcacgccgg accggaaggt tgccgaaaag gaatccgcca
     8101 gggaggcggc cgcgcgcgag cagcaggcgc tggcggatcg cgccgccacg ctggaagaat
     8161 ccctggagtc gaagcgcctt gaaaacaagg agctcagcga caaggtcggc ttggtgcgtt
     8221 cgcagctcaa gaaagaatcg cgtctgatcg aactggaaaa ccagagcctg gcgcagcagc
     8281 cgaaaccggc cgaaaccaaa ccggcggaac ccgccgtcaa gccggcagag cccgctccca
     8341 agccggtgga aaaagccacc gcgcctgtgc cggaacagca ggccaaaatt gcagcgctgc
     8401 caccggccaa gcccgcgccc gccgcgccca agaagaaggc gtcattgcca ccgccgccgg
     8461 aagaaaaaga tttcctcgcc acggtgctgg aggccgtgca aagcgatctg ctggtgccgg
     8521 tcgtgatcgg tgtcgtggtg ctgctcagcg gcggtatcgc gctggtttat ttccgccggc
     8581 gccagaaatc catcgctgaa ttcgaggaaa gcattctctc ggccgatgcc atcagcaccg
     8641 agcagccggc gaccaccggc accgtcgtgg gccaacaacc ggtggctgct gcgactaccg
     8701 ggggcgatac ttcgttcctg agcgacttca g
//
LOCUS       DBNGZE010000442         3422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1867241, whole genome shotgun sequence.
ACCESSION   DBNGZE010000442 DBNGZE010000000
VERSION     DBNGZE010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1867241"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="AC16FV_11565"
     CDS             complement(<1..140)
                     /locus_tag="AC16FV_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR2850722.1"
                     /translation="MTLDARAEILLKTLIERYITEGQPVGSRTLARQAGLDLSSATVR
                     NV"
     gene            379..1257
                     /locus_tag="AC16FV_11570"
     CDS             379..1257
                     /locus_tag="AC16FV_11570"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HIR2850723.1"
                     /translation="MDKKIKTVGLFGKYQDQTVIDHLRRLEEFLLGRKLKTLLDHATA
                     EHFPGAKSPSRPRADIGNEIDLAIVVGGDGTLLNVARELAPHRVPIIGVNLGRLGFLT
                     DIQADSMTTEIGKILDGDYQTENRLLLYAEVMRKGRIVHAASAFNDVIVAKGELARMI
                     EFETYVDGEFVHSIRGDGIIVASPTGSTAYALSSGGPILHPTLDAIAIVPICPHTLSN
                     RPIVISSDSIVEILVSRIAEQHAYSAFDGQSTFALQDNDRVYVRRAEHEVELLHPSGR
                     SHFEVMRIKLHWGRKL"
     gene            1257..2930
                     /gene="recN"
                     /locus_tag="AC16FV_11575"
     CDS             1257..2930
                     /gene="recN"
                     /locus_tag="AC16FV_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR2850724.1"
                     /translation="MLTQLYVRDFAIVRELSLAYEPGFTVLTGETGAGKSILIDALAL
                     VLGERADSEVIRHGCARAEVTAGFALKPSSSAAKWLKENDLFDDQECVLRRVIEADKP
                     SRGFINGRPVPIQMLRELGEYLVDIHGQHEHQSLLRRDAQRQILDDYAGLDESVRMLT
                     GHYREMENLRERLATLKQQTADREARIEWLTHQVRELDALALTTDEIPALEEEHARLA
                     NGAELIEGVQSLAQTIQEDEEAAVSQQLARAINRLESLVKYDAKLGDIAALLNEAAIQ
                     IDEAGSRLNHYLESLELDPSRLQIVERRLSTVHDLARKHKVKPEELPAVHARLRAELN
                     EIENLDTSLERLEQDLEKARTSYFKTAKDVSRTRQGAAKKLSKAVTGEMQELGMPGGS
                     FEVALVSLPEAEAGAHGLERVEFLVSANPGQPARPLAKVASGGELSRISLALQVVLAG
                     TGRIPTLIFDEVDVGIGGRVAEVVGRKLRALGESRQVLCITHLAQVAAQGMQHVQVRK
                     QTEGKDTVAEAVPLAERERTMEIARMIGGVEISKQTLAHAKDMLERASA"
     gene            2917..3414
                     /locus_tag="AC16FV_11580"
     CDS             2917..3414
                     /locus_tag="AC16FV_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR2850725.1"
                     /translation="MPQPDLNRAAQSAVSTIRPAAISDVPYIHHLLEIYAAQGNLLPR
                     TMNELYRHLRDFFVVEVNGRVAGCGALEIFTESLGEVRSLVVDDAWKGQGFGRMLVQR
                     IIEEARAIGLKRLMALTYVAPFFHKLGFKTVPMDTLPEKVWGICVKCYKYNNCDETAV
                     LLELK"
BASE COUNT          739 a         1063 c         1031 g          589 t
ORIGIN      
        1 atgacattgc gcacggtggc cgaggacaaa tccagcccgg cctgacgcgc cagggtgcgc
       61 gaacccacgg gctgaccctc ggtgatatac cgctcgatca gggttttcag caatatttcc
      121 gcgcgtgcgt cgagagtcat ggcaaatctc tataatggtg ctgactttag cactctcata
      181 gcttgagtgc caagcccggt acccccgcaa gaatttgtca caaaataaaa cagactggac
      241 caacaggatt tcactggatt cacaggatta aaagcagatg atcgcgcaag tcacgttcgc
      301 tatccgcgat caataaatag tgcattggcc cgcggtttgg tgcgtgctag gtttatgatt
      361 cacatccgga agatacacat ggacaaaaaa atcaaaaccg tcggcctgtt tggcaaatac
      421 caggaccaga ctgttatcga tcacctgcgc cggctcgagg aattcctgct gggtcgcaaa
      481 ctcaagaccc tgctggacca tgccacggcc gagcatttcc ccggcgccaa gtcgccatcg
      541 cgaccgcgcg cggatatcgg caatgaaatc gatctggcca tcgtcgtcgg cggcgatggc
      601 accctgctca acgtggcgcg cgaactcgcc ccgcatcgcg ttcctatcat cggggtcaat
      661 ctcggccggc tcggcttcct caccgacatc caggccgaca gcatgaccac ggagatcgga
      721 aaaattctcg acggcgatta ccagaccgaa aaccggctgt tgctgtacgc cgaagtcatg
      781 cgcaaggggc gtatcgtaca cgccgccagc gccttcaacg acgtgattgt cgccaagggc
      841 gagctcgcgc gcatgatcga gttcgagacc tatgtcgacg gcgaattcgt ccacagcatc
      901 cgcggcgacg gcatcatcgt cgcgagcccg accggctcca ccgcctacgc gctttcatcc
      961 ggcgggccga tcctgcatcc cacgctcgac gccatcgcca tcgtgccgat ctgcccgcac
     1021 acgctctcga accggccgat cgtgatttcc agcgacagca tcgtggagat cctggtctcg
     1081 cgcatcgccg aacagcacgc ctacagcgcc ttcgacggcc agtccacctt tgcgctgcag
     1141 gacaacgatc gcgtgtacgt gcgccgcgcc gaacacgagg tcgagctgtt gcacccctcg
     1201 ggccgcagcc acttcgaggt gatgcgcatc aaactgcact ggggccggaa actctgatgc
     1261 tgacacagct ttacgtccgc gatttcgcca tcgtgcgcga actgagcctg gcgtacgaac
     1321 cgggcttcac ggtgctgacc ggcgaaaccg gcgcgggcaa atccattctc atcgacgccc
     1381 tggcgctggt gctgggcgaa cgcgccgaca gcgaagtgat ccgccatggc tgtgcgcgcg
     1441 cggaagttac ggccggcttt gcgttgaaac cctccagcag cgccgccaaa tggctcaagg
     1501 aaaacgatct gttcgatgac caggaatgcg tgctgcgccg tgtcatcgag gccgacaaac
     1561 cctcgcgcgg attcatcaac ggtcgtccgg tcccgatcca gatgctgcgc gaactgggcg
     1621 aatatctcgt ggacattcac ggtcagcatg aacaccagtc gctgctgcgg cgcgatgccc
     1681 agcggcaaat cctcgatgac tacgccgggc tggatgaaag cgtgcgcatg ctcaccggcc
     1741 actaccggga aatggaaaat ctgcgcgaac ggctcgcgac gttgaaacaa cagacggcgg
     1801 accgcgaggc gcgcatcgag tggctcacgc accaggtgcg cgaactggac gcgctggcac
     1861 tgactactga cgagattccc gcgctcgagg aagagcatgc gcgcctggcc aacggcgccg
     1921 aactcatcga gggcgtgcaa tccctggcgc agaccataca ggaagacgag gaagccgcgg
     1981 tatcgcaaca attggcgcgc gctatcaacc ggcttgagtc gctcgtcaaa tatgacgcca
     2041 aacttggcga catcgccgcc ctgctcaatg aggcggcgat ccagatcgac gaggcaggca
     2101 gtcgccttaa tcattatctc gaaagcctgg agctcgaccc gtcgcggctg cagattgtcg
     2161 aacgccggct gtccaccgtg catgacctgg cgcgcaagca caaggtcaaa ccggaggaac
     2221 tgcccgcggt gcacgcgcgc ctgcgcgcgg aattgaatga gatcgaaaat ctcgacacca
     2281 gcctggaacg actggagcaa gatctcgaaa aagcacgcac gtcctacttc aagacagcca
     2341 aagacgtttc gcgtacacgc caaggcgcgg ccaaaaaatt gtcgaaagcg gtcaccggcg
     2401 agatgcagga actcggcatg ccgggcggga gcttcgaagt cgcgctggtt tcattgccgg
     2461 aagcagaagc cggcgcccac gggctcgagc gcgtggaatt cctagtcagc gccaaccccg
     2521 gccagccggc gcggccgctg gccaaggtgg cctcgggcgg cgaactgtcg cgtatcagcc
     2581 tcgcgctgca ggtggttctt gcgggcacgg ggcgcatccc cacgctgatt ttcgacgaag
     2641 tagatgtcgg tatcggtggg cgcgtggccg aggttgtggg tcggaaactg cgcgcactgg
     2701 gcgaatctcg ccaggtgctg tgcatcaccc acctggcgca ggtggcggcg cagggcatgc
     2761 aacacgtgca ggtgcgcaaa caaaccgaag gcaaggacac ggtggccgag gcggtcccgc
     2821 tcgccgaacg cgagcgcacc atggaaatcg cgcgtatgat tggcggcgtg gaaatcagca
     2881 aacagaccct ggctcacgcg aaggacatgc tcgaacgtgc ctcagcctga cctgaaccgc
     2941 gccgcgcaga gcgccgtgtc gacgatccgt ccggccgcga tcagcgacgt gccttatatt
     3001 catcatctgc tcgaaatcta cgccgcgcag ggcaacctgc tgccgcgcac gatgaacgaa
     3061 ctctaccgcc atctgcgcga ttttttcgta gtcgaggtaa acggccgggt cgccggctgc
     3121 ggcgcgcttg aaatattcac cgaaagcctc ggcgaggtca ggagtctggt ggtggacgac
     3181 gcctggaagg gccagggatt cgggcggatg ctggtgcagc gcatcatcga ggaggcgcgc
     3241 gccattggcc tgaagcggct gatggccctg acctatgtcg cgccgttttt ccacaaactc
     3301 ggcttcaaga ccgtgcccat ggacacgcta ccggaaaagg tctggggtat ctgcgtcaag
     3361 tgttacaaat acaataactg cgatgaaacc gcggtattgc tggagctgaa ataaatcagg
     3421 gt
//
LOCUS       DBNGZE010000443         3292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1311225, whole genome shotgun sequence.
ACCESSION   DBNGZE010000443 DBNGZE010000000
VERSION     DBNGZE010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1311225"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1978)
                     /locus_tag="AC16FV_11585"
     CDS             complement(47..1978)
                     /locus_tag="AC16FV_11585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR2850726.1"
                     /translation="MITRRTFLKLSVTGAGLFLLTKFGSPRAFAAIPGGTLNLKKVSK
                     YQMPLIIPPAMPRTSEINVQGAVDYYEIAVRQFQQQILPPPLPPTTVWSYGSASHPGS
                     FNYPAFTIEAKWHKPVRVKWINDLKDPVTGNYLPHLLPVDPTLHWANPPGGTAGRDMR
                     PTFTSTPGPYAGPVPIVTHLHGGHSTQESDGYAEAWFLPAANNVPAGYATEGSWYDSF
                     KAQFQSQQGVAWEAGTAVFQYANDQRAATLWYHDHTLGMTRLNVYAGPAGFFLLRGGP
                     GDKVIGTLPEPAPALGDPLGMKYYEIPIAIQDRSFNKDGSLFYPDSRKFFDGFKGPYI
                     PESDVSPIWNPEFFGNAMVVNGRAWPFLEVEPRRYRFRLLNGCNSRFLILKFSDPNLS
                     FWQIGSDGGFLPAPVQLSQLLMSPAERMDVIVDFSGLAPGTEILLQNIAPDEPFGGGV
                     PGVDFPVSDPETTGQVMQFRVVPLTAADTSTPPDLLGLPAFVGLGPVSNTRQVSLNEE
                     SSKFPGFDGPVEAELGILDSAGNPVHLGWDKPITENPALGATEVWEIHNFTEDAHPIH
                     IHEVQFQIVDRQPFEGDPRPPEVNETGFKDTIIAYPGEITRVKAKFDLPGLYVWHCHI
                     VEHEDNEMMRPYFIGPMAP"
     gene            complement(2506..3201)
                     /locus_tag="AC16FV_11590"
     CDS             complement(2506..3201)
                     /locus_tag="AC16FV_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR2850727.1"
                     /translation="MKTSDIKTDEYRQSMGQLLMLYTQVDRLIMEVCAERLAQAPDED
                     AELSLAKQVGDESRHVSIQRQWMHEFGTRPTPVITTEQEQMIRAHFRSLPWIDFLADL
                     YICVEALGSEAVERIVPLADPGTRESLRVPLTDEIDHVAFGVSRLKQELAQLSETERK
                     VFLERLPVRIEALTDAFHGFGFSVRDMFEAVGADYDRLCRLLEERQKELIAELAFGKQ
                     PPQPARVASDVLA"
BASE COUNT          610 a         1007 c         1011 g          664 t
ORIGIN      
        1 cgggtcagcg ccactcaggc gctgacccgc cataacctct gttgcgctac ggcgccatcg
       61 ggccgatgaa gtacggccgc atcatctcat tgtcctcatg ctcgacgatg tggcagtgcc
      121 agacatacag ccccggcaga tcgaacttcg ccttgacccg cgtgatttcg cccggatagg
      181 cgatgatggt gtccttgaag cccgtctcgt tgacctccgg cgggcgtgga tcgccctcga
      241 atggctgacg atcgacgatc tggaactgca cctcgtggat gtggatggga tgtgcatcct
      301 ccgtgaaatt gtgaatctcc cacacctcgg tggcgcccag ggccgggttt tcggtgatcg
      361 gcttgtccca acccagatgg accgggttgc ctgcgctgtc gagtatgcct agctccgctt
      421 caacggggcc gtcgaagccg gggaacttac tcgattcttc gttgagcgac acctgccgtg
      481 tattgcttac cggaccaagt ccgacaaacg cgggcaaccc cagcaagtcg ggcggggtgc
      541 tggtatccgc tgccgtcagc ggcaccaccc ggaactgcat tacctggccg gtcgtctccg
      601 ggtccgagac tgggaaatcc acccccggca cgccaccgcc gaagggctca tccggggcaa
      661 tattctgcag gaggatctcg gttccaggcg cgaggcccga gaagtcgacg atgacgtcca
      721 tccgctccgc cggtgacatc aggagctgcg acaactgcac cggcgcaggc aggaagccgc
      781 cgtcgctccc gatctgccag aagctcagat tcggatcgct gaatttcagg atcaagaagc
      841 gcgagttgca gccgttcagc aaacggaagc ggtagcgccg cggctcgact tccagaaagg
      901 gccaggcgcg accgttgacc accatggcgt tgccgaagaa ctccggattc cagatgggcg
      961 agacatcact ctcgggaata tacggtccct tgaagccatc gaaaaatttg cggctgtccg
     1021 ggtagaacag cgagccgtct ttgttgaagg agcggtcctg gatcgcgatt ggtatctcgt
     1081 agtacttcat gcccagcggg tcgccgagcg ccggcgccgg ttccggcagt gtgccgatca
     1141 ccttgtcacc cggaccaccg cgcagcagga agaagcccgc cggcccggcg tagacgttca
     1201 gacgcgtcat gcccagcgtg tgatcgtgat accacagcgt ggccgcgcgc tggtcgttgg
     1261 catattggaa cacagccgtg ccggcctccc aggctacgcc ctgctggctt tggaattggg
     1321 ccttgaagct gtcgtaccag ctgccttcgg tggcatagcc tgcgggaaca ttgttcgccg
     1381 ccggcaggaa ccaagcctca gcatagccgt cgctctcctg cgtcgaatgg ccgccgtgca
     1441 aatgcgtcac gatcggcacc ggaccggcat aaggacctgg cgtgctcgtg aatgtggggc
     1501 gcatgtcgcg accggcagtg ccgccgggcg ggttggccca gtgcagcgtc gggtccaccg
     1561 gcaggagatg cggaagataa ttacccgtca ccggatcttt caggtcgttg atccatttga
     1621 cccgcaccgg cttgtgccac ttggcctcga tggtgaaggc gggataattg aacgaaccgg
     1681 ggtggctagc cgagccgtag ctccacacgg tggtcggggg caacggcggc ggcaggatct
     1741 gctgctggaa ctgtcggacc gcgatctcgt agtagtcgac ggcgccttgg acgttgatct
     1801 cgctggttcg cggcatcgcc ggcggtatga tcagcggcat ctggtatttc gagaccttct
     1861 tgaggttcag cgtgccaccg ggaatcgcgg caaaggcacg tggggacccg aacttcgtca
     1921 acaagaacag ccccgcacct gtcacgctca gcttaagaaa tgttcgtctg gtgatcatcg
     1981 ttctctcctc cttgttttat cctccgaacg caatgcgtcc ttcggattga ccatggccga
     2041 atgtcttggt gtctggattg tgtgcgggca cccccttcgc tccttcagag cgaggaccgg
     2101 ctggtgatgc cggtgtaatt ctattttccg ccgaaccccg ctgacgcgat gcgtccatcg
     2161 gacccggctt ggcactcctc tgcgcttgcc ttcagtccct ggcttaattg ctaaaccaaa
     2221 gcttcctttt attacttaag gagtctgcat atcaggccag caatcttctg acgtgtcaac
     2281 gcgtgcataa atatcgttga atatccatgc tctttatccg agaagcgggc agcatcggag
     2341 ctgtcccgcg cacggaattg cacgacgcga gctcgccgcc gaacgggcgc aaggggtttg
     2401 gttgtaccct gagctgaaag gttgggcggt atttgccgtt acccacccta aagaattcgg
     2461 cgggttatgc gccagacgca ggcgctaacc cgccctacat tattcctagg ccaagacgtc
     2521 cgatgcgacc ctcgccggtt gcggcggctg cttcccgaac gccagctcgg cgatcaactc
     2581 tttctggcgc tcctccagca aacggcaaag ccggtcgtag tccgcgccga cggcctcgaa
     2641 catatcgcgc acggaaaaac cgaaaccgtg gaaggcatcc gtcagtgcct cgatgcgcac
     2701 gggcaggcgc tcgagaaaca ctttgcgctc ggtttcggaa agctgcgcca gctcctgctt
     2761 caaccgcgac acgccgaagg cgacgtgatc gatctcgtcg gtgagcggga cgcgcagcga
     2821 ttcgcgcgtg ccgggatcgg cgagcggcac gatgcgctcc accgcctcgc tcccgagcgc
     2881 ttccacgcaa atatatagat cggccaggaa atcgatccac ggcagcgagc gaaaatgcgc
     2941 acgaatcatt tgctcctgtt cggtcgtgat gaccggagtc ggccgggttc cgaactcgtg
     3001 catccattgg cgctggatgg aaacatggcg gctttcgtcg ccgacctgct tggccagcga
     3061 cagctccgcg tcctcgtcgg gcgcctgcgc cagacgttcg gcgcagactt ccatgatgag
     3121 gcgatcgact tgcgtgtaga gcatgagcag ctggcccatg gattggcgat attcgtcggt
     3181 tttgatgtcg gatgttttca taggactttt ctcctcaggt tgggaatgcc ccaactggtg
     3241 ttggggcgcc ggaacttggt acggctggcg gcgcgcatgg cggaaaaccg ca
//
LOCUS       DBNGZE010000444         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_618472, whole genome shotgun sequence.
ACCESSION   DBNGZE010000444 DBNGZE010000000
VERSION     DBNGZE010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_618472"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC16FV_11595"
     CDS             <1..714
                     /locus_tag="AC16FV_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR2850728.1"
                     /translation="ARLLVRSAEGSLGAWIALFTFAVTAAVTQFGALAGAREHLVGLS
                     AISLSAGDSSLAVLLHIPPGVAGFVVAALCLVWLVMAGRGTHDRRLIIGGAAIGALVV
                     LAWWVTGNLAWDEFTHIPPVALNISGPLARIAHALVTGNSFGTGFGIPFVVGTIAGAM
                     LSAFLGGALRWQAPQARYIPNHLAGGVMMGAGAVLAGGCNIGQGLSGVSTLAIGSLAA
                     AGAIFIGAVLGVKWLERNA"
     gene            868..1095
                     /locus_tag="AC16FV_11600"
     CDS             868..1095
                     /locus_tag="AC16FV_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850729.1"
                     /translation="MSTVTLLVILVVWSVAGLLAAIAFGRVIQDTSPDEEENLASSAG
                     SVKYIRRNKDKTRDASVNVSGSRRSTKHASG"
     gene            complement(1092..1775)
                     /locus_tag="AC16FV_11605"
     CDS             complement(1092..1775)
                     /locus_tag="AC16FV_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HIR2850730.1"
                     /translation="MSDETILLGMKAIEVWPKDCQSEVCGDPTIYKITFTDTQEYHPR
                     LVAKILELEENHRLRKRYFRGACGTKIHHLEQWASTEAELITARAKALFRKVLECEHA
                     VIDLSWANVYRNGDYCLPHSHLRSTASVVYCLDPGDEDPEDSQSGRFCFVDPRLRNCC
                     QHQEGCMTTPFFPGMAAGTMLIFPSQLVHSVNPYTGKRPRITLSWNINKHALPGSPLP
                     ANGGAIREI"
     gene            complement(1856..2236)
                     /gene="acpS"
                     /locus_tag="AC16FV_11610"
     CDS             complement(1856..2236)
                     /gene="acpS"
                     /locus_tag="AC16FV_11610"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR2850731.1"
                     /translation="MIYGIGTDIVRVARMQHDIERFGERFAERILTATEMQEYRQNTN
                     KANFLARRFAAKEAAAKALGTGFSQGVQLHDIEVTHDTQGKPLLEFRGRAREFLREKN
                     ISIAHISLADERDHAVAFVTLETG"
     gene            complement(2337..>2540)
                     /locus_tag="AC16FV_11615"
     CDS             complement(2337..>2540)
                     /locus_tag="AC16FV_11615"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR2850732.1"
                     /translation="QQGLDLKLQVNAGHGLNYHNVQSIAAIPGISELNIGHAIIARAL
                     FTGLPEAVREMKRLMNEATIKNP"
BASE COUNT          477 a          714 c          757 g          592 t
ORIGIN      
        1 gcgcgcctgc tggtgcgcag cgccgaaggc agtcttggcg cctggatcgc cctgttcact
       61 tttgccgtga cggcggccgt cacgcagttc ggcgcgctgg ccggtgcgcg cgaacatctc
      121 gtcggactgt ccgctatttc tttaagcgcc ggtgattcct cgttggccgt actgctccat
      181 atcccgcccg gtgttgccgg ctttgtcgtt gccgctctgt gcctggtctg gcttgtcatg
      241 gctggacgag gcacgcatga tcggcgtctg attatcggcg gagcggctat tggtgcgctg
      301 gtggtcctgg cctggtgggt gacgggaaat cttgcttggg atgaattcac ccacattccg
      361 cccgtcgctc tcaatatctc cggcccgctc gcccgcattg ctcatgctct ggttaccggg
      421 aacagtttcg gtaccggatt cggaattcca ttcgtggtcg gtaccattgc gggcgccatg
      481 ctcagcgctt ttcttggtgg cgccctgcga tggcaagcac cgcaggcacg ctatatccca
      541 aaccatctcg ctggcggggt catgatgggg gccggcgccg tactggcggg tggatgcaat
      601 attggccaag gcttaagcgg tgtttccacg ctggcgatcg ggtcgcttgc cgccgccggc
      661 gccattttta tcggagctgt gctcggcgtg aaatggctcg aacggaatgc ttaatcttgt
      721 tgagatgatc attttactca agtaatgggc gccgaccgtt tgtcaggaaa caggtaccgt
      781 tggagacaga aacagcgcca gatgggttgt attcgccggt ggtttaaggg attctgctga
      841 cgctgttact tacacggagg gtaattaatg agcacggtca cacttctcgt tatcctagtg
      901 gtatggtcag tcgccggctt gttggcggcc atcgccttcg gcagggtcat acaggacacc
      961 tcgcccgatg aggaggaaaa cctcgcctcc tccgccggct cggtcaaata catccgccga
     1021 aacaaggaca agactcgcga cgcctcggtg aacgtatcgg gttcgcgccg cagcacaaaa
     1081 cacgcctcag gctagatttc acggatcgct cccccattcg cggggagcgg cgatccgggc
     1141 agcgcatgct tgttgatatt ccacgataag gtaattcgcg gccttttccc cgtgtaagga
     1201 ttgacggaat gcaccagctg gctgggaaat atcagcatgg tgccagccgc catgccggga
     1261 aaaaacggcg tggtcatgca accctcctga tgctggcagc agttgcgcag acgcggatcg
     1321 acaaaacaaa agcgccctga ctgggaatcc tccgggtcct cgtcaccggg gtccaggcag
     1381 tacaccacgc tggccgtaga gcgcaggtga ctgtgcggca ggcaataatc cccgttccgg
     1441 tacacattgg cccagctcaa atcgataacc gcatgctcgc attcgagcac cttccggaac
     1501 agggccttcg cgcgcgcggt aatcagctcg gcctcggtgc tggcccactg ctccaggtga
     1561 tgaatcttgg tgccgcacgc accgcggaaa tagcgcttgc gcaggcggtg gttttcctcg
     1621 agctcgagaa tcttggcgac cagacgcggg tggtattcct gagtatcggt gaaggtgatc
     1681 ttgtagatgg tgggatcacc gcatacctct gactgacagt ccttcggcca cacttcgatg
     1741 gccttcatgc ccagcaggat ggtttcgtcg ctcatcgggt tcctgtgtct cgcaaccgct
     1801 ggcgacgtat tctgtcatgt aatggggtag agaggaaacc ccgcgcggga atccatcagc
     1861 cggtttcgag cgtcacgaaa gcgacggcat gatcccgctc gtcggcaaga ctgatatgcg
     1921 cgatggaaat gtttttttcg cgcaggaact cccgggcccg gccgcggaat tccagcaagg
     1981 gcttgccctg cgtgtcgtgg gttacttcga tatcatgcag ttgcacgccc tggctgaaac
     2041 cggtgccgag ggccttggcc gcggcttctt tcgctgcgaa acgacgcgcc aggaaattgg
     2101 ccttgttggt gttctggcga tactcctgca tttcggtggc agtcagaatg cgctcggcga
     2161 aacgctcgcc aaaccgctcg atatcatgct gcatgcgcgc gacgcgcaca atgtcggtgc
     2221 cgatgccgta aatcatttta agtctttatg caaatcaaat tcacaggatt gacaggatta
     2281 aagattcaga atttaagaaa tcctgttaat cgtgttttta atcctgttaa tcctgtctat
     2341 ggatttttaa tagttgcctc gttcatcagg cgcttcattt cgcgcacggc ttccggcagg
     2401 ccggtgaaca gggcgcgcgc aatgatggcg tgaccgatgt tcaattctga aatgcccggg
     2461 atcgcggcga ttgattgaac attgtgataa ttcaggccat gaccggcgtt cacttgcagc
     2521 ttgagatcga gcccctgctg
//
LOCUS       DBNGZE010000445         6284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1242514, whole genome shotgun sequence.
ACCESSION   DBNGZE010000445 DBNGZE010000000
VERSION     DBNGZE010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1242514"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC16FV_11620"
     CDS             <1..210
                     /locus_tag="AC16FV_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HIR2850733.1"
                     /translation="RAMVTRPLCVLADEPTGNLDRENAQNVYELMLELNRELKTSFII
                     ATHDPALAASMDRVLKLEDGVLKSA"
     gene            complement(207..737)
                     /locus_tag="AC16FV_11625"
     CDS             complement(207..737)
                     /locus_tag="AC16FV_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019440303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR2850734.1"
                     /translation="MAKKLIRRYLPDPHKIRDHKHLRLFGTLLHDPNLWHLNRRSASG
                     AFAVGLFMAFVPMPFQMIPAAALAILFRVNLPIAVALVWLTNPLTIAPVSYFCYYLGA
                     WILDTPVHAVEFQISWEWISTELIRIWKPFLLGSLLVSTMSAAAGYFGIRALWRWHVI
                     RDWEKRKKKRTKTASS"
     gene            806..3073
                     /locus_tag="AC16FV_11630"
     CDS             806..3073
                     /locus_tag="AC16FV_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR2850735.1"
                     /translation="MAFLGGVLLVQQLAALPSLWWTLLLAPLLWQAFRRHPLWFVPVF
                     FVTGVMWASFRAGIILDDSLPRELEGRDLVVEGRIVDIPRKAEFGQRFELEVTHAELD
                     GAPVHVPRKILLNSRGYSFQPRAGETWRLLARLSRPHGYQNPGGFDYEAHLFRERIRA
                     KGYARDDMAPLHLEREPAWYDIDRWRQDLGDSIRALLHDNEYAGIVVALANGDGRGLS
                     EQQWQVLRRTGTLHLVAISGLHISLIAGVVFFLARWLWALPGTTVLRLPAPQVGAICA
                     LLAAGFYSALAGFVVPTQRALIMLTVAMAGILLRRRFPPSQLLAVAGLAVLLYDPLSV
                     MAAGFWLSFAAVAVILFVMHGERSGRGLAWKLGYIQWAIALGMLPLMLAMFQQVSLVA
                     PLANMLAVPVFDLMAVPLTLLGTILLGIAPDVAGLFFQLAAWLLHWLWQALTWLADLS
                     FSQWAQPAPTWWALLCGVVGVLLLLAPRGWPTRWVGAVWMLPLFLVRPPQPATGEVWF
                     TLLDVGQGLAAVVRTHEHALLFDAGPRFGDFDTGKAVVEPYLRAKGVRRLDAFVISHK
                     DTDHIGGAESVLHELPVVKMLSSVPEISPMAQPCRAGQSWNWDGVDFAVLGPDEAGAE
                     RLNDASCVLQVRSRYGNILLPADIEARAEKKLVEAWGERLRSEILVAPHHGSKTSSTP
                     AFVEAVAPRHVLFPAGYRSRYRHPHPDVVRRYAERAVILHDSAASGALEMQLNATGFD
                     IAAYRACHRRYWYAD"
     gene            3175..3789
                     /locus_tag="AC16FV_11635"
     CDS             3175..3789
                     /locus_tag="AC16FV_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR2850736.1"
                     /translation="MFEIIKAGGWSMWPIILCSVAAMAIIGERFWSLQKKYVVPSNLL
                     VQVRQLLERNELESSHLAALRGSSPLGKILAAGLVNRDHDREIIKEAVENAGRHVVPD
                     LERYLRTLGSIAAISPFLGLFGTVIGMIQMFSGIGQHGVGDPSIVAEGIAVALINTAA
                     GLAVAIPSLLFYRYFRGRVSDLLIEMEEEAVKLVEILHGEREND"
     gene            3800..4228
                     /locus_tag="AC16FV_11640"
     CDS             3800..4228
                     /locus_tag="AC16FV_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2850737.1"
                     /translation="MNFRPTPDEEPEINLIPLIDVLLMALIFLIVTTSFSTESRLNVR
                     LPEASAEVKENLPSLRVTIDAKGQFYIGDQQLLNATPEVLRNALLRAAAGNKDPLIVI
                     HADAHTPHEAVIRAMDAARRLGFTRLTFATQQPSGQAARE"
     gene            4221..5975
                     /gene="msbA"
                     /locus_tag="AC16FV_11645"
     CDS             4221..5975
                     /gene="msbA"
                     /locus_tag="AC16FV_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR2850738.1"
                     /translation="MSKIPDTGNKLRLYRRLLAYAWPYKWVFLVAVVGMIVLSATAAG
                     FTAVMKPLMDEGFVQRDASTIKMIPLLIVLLFLARGIGNYLAQYGTAWIGRRVTFDLR
                     NSIFRRMMYLPSRYYESSASGGLISRIIFDVEQIAAGVTQVPYVVIGDGLTMLALAGW
                     LLYLNWKLTLVFAVLLPVTALLMRAMNRRFLRTSIEIQTSMGEISQVVREASEGQRVV
                     KAFGGQPAEIRAFERGNEKNRRQSLRKAAVSAIGMGLLQLAGSIALGLVIYTALLSGE
                     ITAGTFTSYLVAALWIMGPSRRLARINETIQTALAAAQSAFAVLDEMPEEDTGSVTIE
                     RARGRVEYRQVGFRYPNADRDILDDVSFTVEPGQVLALVGASGSGKSTIVSLLPRFYR
                     AGRGAIRLDDTDINDLTLASLRRQVALVGQENILFDDSIRHNIAYGEEGAIDDARLME
                     AARAAHVLEFAQRLPEGLDTHVGERGALLSGGQRQRIAIARALYKNAPILILDEATSA
                     LDTESERLVQAALQKLMEHRTTLVIAHRLSTVEHADKIVVLAQGRVVETGSHRELLAR
                     NGAYAALYRNQFSDKPAN"
     gene            5986..>6284
                     /locus_tag="AC16FV_11650"
     CDS             5986..>6284
                     /locus_tag="AC16FV_11650"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR2850739.1"
                     /translation="MARRASGASRATFSHWLEWHWYRKTPLSLLLLPVSWIYCLLILL
                     RRGLYRLGVFPSVKLPVPVIVVGNITVGGSGKTPLVLWLASFLRQKGLRPGIVLRG"
BASE COUNT         1070 a         1885 c         2044 g         1285 t
ORIGIN      
        1 cgcgccatgg tgacgcggcc gttgtgcgtg ctggcggatg aaccgaccgg caacctcgac
       61 cgcgaaaacg cgcagaatgt ttatgagctg atgctggagt tgaaccgcga actcaaaaca
      121 agtttcatca tcgccactca cgatcccgcg ctggccgcca gcatggaccg cgtactcaaa
      181 ctggaagacg gcgtacttaa atctgcctag gacgaagcgg ttttagtcct tttctttttt
      241 cttttttccc agtcacggat cacgtgccag cgccacaggg cgcgtatgcc gaaatagccg
      301 gcggcggcgc tcatggtgct caccagcaag gagccgagca gaaacggttt ccagatgcgg
      361 atcagttccg tgctgatcca ctcccacgag atttgaaatt cgaccgcatg caccggcgta
      421 tccagtatcc aggctccgag gtagtagcag aagtaagaca ccggcgctat cgtgagggga
      481 ttggtgagcc acaccagcgc caccgcgatc ggcaggttca cgcgaaacag gatggccagg
      541 gccgcagccg gtatcatctg gaacggcatg ggcacgaagg ccatgaacag tccgacggcg
      601 aatgcgccag acgccgagcg gcggttcagg tgccacaggt tgggatcgtg cagcagcgta
      661 ccgaacaggc gcaaatgctt gtggtcgcgg atcttgtggg ggtccggcag gtaacgcctg
      721 atcaattttt ttgccatagg tgactggtgt atagtctcac gaaataaaaa taacagccaa
      781 ggagactgcc atccgcgccg ccacattggc cttcctcggc ggcgtgctgc tggtgcagca
      841 gctggccgcg ctgccgtcct tgtggtggac gctattactg gcgccgctcc tgtggcaggc
      901 attccgccgt catcccctgt ggttcgtgcc ggtgttcttc gtgaccggcg tgatgtgggc
      961 gagcttccgc gccgggatta tcctggacga cagcctgccg cgcgagctcg agggccggga
     1021 cctcgtcgtc gaagggcgca tcgtggacat cccgcgcaag gccgagttcg gccagcgttt
     1081 cgaactggaa gtgacacacg ccgaactcga tggcgcgccc gtgcatgtgc cgcgcaagat
     1141 actgctgaac agccggggat attctttcca gccgcgcgcc ggcgagactt ggcggctgct
     1201 ggcgcgtctc agccgcccgc acggttatca gaatcccggc ggttttgatt acgaagcgca
     1261 cctgttccgc gagcgtatcc gcgccaaggg ctacgcgcgc gacgatatgg cgccgctgca
     1321 tctcgagcgg gaaccggcgt ggtacgacat tgaccgatgg cgtcaggacc tgggcgatag
     1381 tatccgcgca ctgctgcatg acaacgaata cgccgggatt gtcgtggcgc tcgccaatgg
     1441 cgacgggcgc ggtctgagcg agcaacagtg gcaagtgttg cgccgcaccg gcacgctgca
     1501 tctggtcgcc atctccggct tgcacatcag tctcattgcc ggtgtcgtgt ttttcctcgc
     1561 ccgctggctg tgggccttgc cgggcacgac ggttttgcgc ctgcccgcgc cacaggttgg
     1621 cgccatctgt gcgctgctgg ccgccggttt ttattccgcg ctggcgggtt tcgtggtgcc
     1681 gacacagcgc gcactcatca tgttgaccgt ggccatggcc ggcatattgc tgcgacggcg
     1741 tttcccgcca tcacagttgc ttgccgtggc cgggctcgcc gtgctgctct acgatccgct
     1801 ttcggtcatg gccgcgggtt tctggctgtc cttcgcggcg gttgccgtga ttctgtttgt
     1861 catgcacggc gaacgatcgg gaagggggct ggcctggaaa ctgggttata tccagtgggc
     1921 catcgcgctc ggcatgttgc cgttgatgct ggcgatgttt cagcaggtat cgctggtggc
     1981 gccgctggcg aacatgctgg ccgtgccggt gttcgatctg atggcggtgc cgttgacatt
     2041 gctgggcacg atcctgctcg gaatagcccc ggatgtggcc ggattgtttt tccagcttgc
     2101 ggcgtggctg ttgcactggc tgtggcaggc actgacttgg ctggcggatt tatcgttcag
     2161 tcaatgggcg caacccgcgc cgacgtggtg ggcgttgctg tgcggtgtcg tcggcgtgtt
     2221 gctgttgctc gcgccacgcg ggtggccgac gcgttgggtc ggtgccgtgt ggatgttgcc
     2281 attgtttctg gttcgaccgc cgcagccggc gacgggcgag gtgtggttca cgctgctcga
     2341 cgtcgggcag ggattggcgg cggtggtgcg cacgcacgag cacgcgttgt tgttcgatgc
     2401 cggtccgcgc ttcggtgact tcgacaccgg caaggcggtg gtcgagccct atttgcgcgc
     2461 caagggcgtg cgtcgcctcg acgcattcgt catcagccac aaagataccg atcacatcgg
     2521 tggcgcggaa tccgttctgc acgaactgcc ggtggtgaag atgctgagca gcgtgccgga
     2581 gatatcgccc atggcgcagc cgtgccgcgc cggccagtca tggaattggg atggcgtgga
     2641 cttcgcggtg ttggggccag atgaagccgg cgcggagcgg ctgaatgatg cctcgtgcgt
     2701 gttgcaggtg cgcagccgtt acggaaacat cctgctgccg gccgatatcg aggccagggc
     2761 cgagaaaaaa ctggtggaag cctggggcga gcgcttgcgt tcggaaattc tggtggcgcc
     2821 gcatcatggc agcaagactt cctccacgcc ggcgtttgtc gaggcggtgg cgccgcgcca
     2881 tgtgttgttc ccggccggtt atcgcagccg ctatcgtcac cctcatcccg acgtggtccg
     2941 gcgttatgcc gagcgcgctg tcatcttgca tgattccgcc gcgtcgggtg cgctggaaat
     3001 gcagttgaac gcaacgggat ttgatatcgc ggcatacagg gcgtgccatc ggcgctattg
     3061 gtacgcggat tgaaccgtcg cgacatacag tttgcatgcg gctgtctttg cggttttggc
     3121 ctcctctgat acattagccg cacttttttg ctgcttatat tcaagagatt aaacatgttc
     3181 gaaatcatca aggccggtgg ctggtcgatg tggcccatca tcctgtgctc cgtggcggcc
     3241 atggccatca tcggcgagcg tttctggtcg ctgcagaaaa aatacgtcgt gccgtccaat
     3301 ttgctggtcc aggtcaggca attgctggaa cgcaacgaac tcgaatcgtc gcatctggcg
     3361 gcgctgcgcg gcagctcgcc gctcggaaag attctcgcgg ccgggctggt caatcgcgac
     3421 catgaccgcg agatcatcaa ggaagcggtt gaaaacgccg gccgacacgt ggtccccgac
     3481 cttgagcgct atctgcgcac gctcggttcc attgccgcga tctcgccgtt cctggggctg
     3541 ttcggcaccg tcatcggcat gatccagatg ttctccggca tcgggcagca tggcgtgggt
     3601 gatccgtcga tcgtggccga gggcatcgcg gttgcgttga tcaacaccgc ggccgggctg
     3661 gccgtggcca tcccaagtct gctgttctat cgctattttc gcggccgcgt gagcgatttg
     3721 ctgatcgaga tggaagagga ggccgtgaag ctggtggaga tattgcacgg cgaacgcgag
     3781 aacgactgag aagagacgca tgaatttccg ccccacgcct gacgaagaac ccgagatcaa
     3841 ccttattccg ttgatcgacg ttttgctcat ggcgttgatc ttcctcattg tcaccaccag
     3901 cttttcgacc gaatcgcgcc tcaacgtgcg cctgcccgag gcctcggccg aggtgaagga
     3961 aaacctgcca tcgttgcgcg tgaccatcga tgccaagggc cagttttata tcggtgacca
     4021 gcaactcctg aacgccacgc cggaagtatt gcgcaacgcc ttgttgcgcg ccgccgccgg
     4081 caacaaggat ccgctgattg tcattcatgc cgatgcacac accccgcacg aggccgtcat
     4141 ccgcgccatg gacgccgcgc gccgcctcgg cttcacccgc ctgaccttcg ccacccagca
     4201 gccgtccggc caagcagcgc gtgagtaaaa tcccggacac cggaaacaaa ttgcgactct
     4261 accgccggct gctggcctac gcctggccgt acaaatgggt gtttctcgtc gccgtggtcg
     4321 gcatgatcgt gctgtcggcc accgccgccg ggttcactgc cgtgatgaaa ccgctcatgg
     4381 acgagggctt tgtccagcgc gacgcttcca ccatcaaaat gatcccgttg ctgatcgtgc
     4441 tgctgttcct ggcgcgcggg atcggaaact atctggcgca atacggtacc gcctggatcg
     4501 ggcgacgggt gacgttcgac ctgcgcaaca gcatcttccg gcgcatgatg tacctgccca
     4561 gccggtatta cgaatcgagc gcctcgggcg gactgatatc gcggatcatc ttcgacgtgg
     4621 aacagatcgc cgccggcgtc acccaggtgc cgtatgtggt gatcggcgac gggcttacca
     4681 tgctggcgct ggccggctgg ttgttgtacc tcaactggaa actcaccctc gtgtttgccg
     4741 tcctgttgcc ggtcacggcg ctgctgatgc gcgccatgaa ccggcgtttc ctgcgcacca
     4801 gtatcgagat ccagacgagc atgggcgaga tctcgcaggt ggtgcgcgag gccagtgagg
     4861 gtcagcgcgt ggtcaaggcc ttcggcgggc agccggcgga aatccgcgcg ttcgagcgcg
     4921 gcaatgaaaa gaatcgccga cagtccttac gcaaggccgc agtgtccgcc atcggcatgg
     4981 gtttgttgca gttggccggc tcgatcgcgc tcggcctggt gatctacacc gccttgcttt
     5041 cgggcgagat caccgcggga acgtttacct cgtacctggt ggccgccctg tggatcatgg
     5101 ggcccagccg gcggctggcg cgcatcaatg aaaccataca gaccgcactg gcggcggcgc
     5161 agagcgcgtt cgccgtgctg gatgagatgc cggaagaaga caccggttca gtgaccatcg
     5221 agcgagcgcg cggtcgcgtg gaataccgtc aggtcggttt tcgttatccc aatgccgacc
     5281 gggatatcct cgatgacgtt tccttcaccg tcgagccggg gcaggtgctg gcgctcgtcg
     5341 gtgcatcggg cagcggcaag agcaccatcg tcagcctgtt gccgcgtttc tatcgcgccg
     5401 gccgcggcgc catccggctc gatgataccg atatcaacga cctcacgctc gccagcctgc
     5461 gccggcaggt cgcgcttgtc ggccaggaga acatcctgtt cgacgactcc atccggcaca
     5521 acatcgccta cggcgaggag ggcgccatcg acgacgctcg cctgatggag gcggcgcgtg
     5581 cggctcacgt gctcgaattc gcgcaacgcc tgccggaagg gctggacacg catgtgggcg
     5641 agcgcggcgc gctgttatcg ggcgggcaac ggcagcgcat cgccatcgcc cgggcgctgt
     5701 acaaaaatgc cccgatcctg attcttgatg aggcgacctc cgcgctcgat accgagtccg
     5761 agcggctggt gcaggcggcg ctgcaaaaac tgatggagca ccgcaccacg ctggtgatcg
     5821 cgcatcgtct gtccaccgtc gagcacgccg acaagatcgt ggtgctggcg caggggcgcg
     5881 tggtggaaac cggctcgcat cgcgagctgc tggcgcgcaa cggcgcctac gcggcgttgt
     5941 atcgcaatca attcagcgac aaaccggcta attgattcaa ctaatatggc aagacgagcg
     6001 agcggagcga gcagagctac attttcgcac tggctggaat ggcactggta ccgcaaaacc
     6061 ccgcttagcc tgctgctgtt gcctgtaagc tggatttatt gcctgctcat tctgctgcgc
     6121 cgggggttgt atcgcctggg cgtatttcct tccgtgaagt tgccggtgcc ggtcatcgtg
     6181 gtgggcaata tcacggtcgg cggcagcggc aagacgccgc tggtgctgtg gctggcgagc
     6241 ttcttgcgcc agaaggggct gcgtccgggg attgtgctgc gtgg
//
LOCUS       DBNGZE010000446         2805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1311888, whole genome shotgun sequence.
ACCESSION   DBNGZE010000446 DBNGZE010000000
VERSION     DBNGZE010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1311888"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="AC16FV_11655"
     CDS             <1..904
                     /locus_tag="AC16FV_11655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850740.1"
                     /translation="DRECVTSAYFAGSTTQLPPPSAVPVPPAAWLFGSGLIGLYGLFQ
                     RKKYGRGDGKAIAFSWFLFVGFTSLLIVNLVSPPVLASSFAGLGESSRGGFAIEAPAL
                     SADWSAIEISAFSADWSKITGFERTSQASVELLDTPAEFGSNTLVWLTAFLTSVPMLL
                     VILEAITAMGFRKRRTTATAGKEFPRTVVLIPAHNEEGVISRCLDSLSVDLPPNCRLL
                     CVAHNCTDATAEVSRNLGAEVIEVQDAGHGGKPDALKAGLQWLDANPPEVVVIVDADC
                     VVSEGAVRIHATRAWELNRPDMGA"
     gene            935..1648
                     /locus_tag="AC16FV_11660"
     CDS             935..1648
                     /locus_tag="AC16FV_11660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2850741.1"
                     /translation="MATLSSLAVLLKNYIRPLGLRAFGLPCLLNGSGSAYPFQVIRNA
                     PQGKGAIAEDYQLAIDLLERGYPTTFVPEARVDGLLPKRDDTALRQRRRWEHGHMFLA
                     FRTAPRLMLEGLKRLDKNRIGLAMEVAVPPLAFLGLMWTVAAGLSLALYVFYGQGGPL
                     GFLVGMAAIFAVAVLMSWMRFAGIKSTLAALASVPGYLMWKLPMYREFFTRRETRWMK
                     TARDSLNGAQHGLRAPFPG"
     gene            2082..>2805
                     /gene="dapB"
                     /locus_tag="AC16FV_11665"
     CDS             2082..>2805
                     /gene="dapB"
                     /locus_tag="AC16FV_11665"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR2850742.1"
                     /translation="MINIAVTGAAGRMGRTLIEACARAEGLRLSAAMERAGNTAVGKD
                     AGEVAGVGKLGVTVRSGLDGADFSVLVDFTSPEATLANLDYCRQNGKKMVIGTTGLDD
                     AGKSRIAAAGKDIAIVFAPNMSVGVNLCFKLLETAARVLGDDVDVEIVEAHHRHKADA
                     PSGTALRMGEVIARVLQRDLKEHGVYGRHGISGERARKTIGFSSIRAGDIVGDHTVLF
                     AGGGERVEITPRAESRMPFANGA"
BASE COUNT          511 a          781 c          890 g          623 t
ORIGIN      
        1 cgacagggaa tgcgtgacca gcgcctactt cgccggttcg acgactcaac tgcctcctcc
       61 aagcgctgta ccggttcccc ctgctgcatg gctgttcggt tccggattga tcgggttata
      121 tggattgttc cagcgcaaaa aatatggacg tggtgacggg aaagccatcg cgttttcctg
      181 gtttttattt gttgggttta cctctctgtt gattgtgaat cttgttagcc ccccggtact
      241 cgcctcgtct tttgcagggc tgggtgagtc ctcacgcggt ggttttgcca tcgaggctcc
      301 cgccttatct gccgactggt cggccatcga gatttccgcc ttttccgctg attggtcaaa
      361 aataactggt tttgaacgca cgagccaggc atctgtcgag ttgctggata cgccagcgga
      421 atttggcagc aacacactgg tttggctgac ggcgtttctc acatcggtgc cgatgcttct
      481 ggtgatttta gaagccatca cggccatggg tttccgcaaa cggaggacaa ctgccacagc
      541 ggggaaggaa tttccgcgca cggtcgtgct catccccgcg cacaacgaag agggcgtgat
      601 cagtcgttgt cttgattccc tgtccgtcga tctgccgccg aattgccgat tgctttgcgt
      661 cgcccacaac tgcacggatg cgaccgctga agtctcccgt aatctggggg ctgaggtgat
      721 tgaggtgcaa gacgctggcc acggaggcaa gcccgatgcg ctgaaggcgg gacttcaatg
      781 gctcgatgcg aatccgcctg aggtcgttgt gatagtggac gcagactgtg tggtgagcga
      841 aggagcagta cggatacatg ctacgcgggc ttgggaactg aatcgtccag acatgggggc
      901 ttaatacttc gcgcccgccg acgcggggaa ggggttggcc accctatcga gcctggctgt
      961 tctgcttaag aactatattc gtcccctggg actgcgcgcc ttcggcctgc cgtgcctgct
     1021 taatggcagc ggatccgcct atccgtttca agtcatcagg aatgcaccgc aaggcaaggg
     1081 cgccatcgcc gaggattacc agctggccat cgatttgctg gagcggggat atccgacaac
     1141 ctttgtcccg gaggcaaggg tggacggact gttgccgaaa cgcgatgaca cggctctgcg
     1201 ccagcgacgc cgctgggagc atggccacat gtttctggca tttcgtaccg cgccgcggct
     1261 catgcttgaa ggcctgaaac ggctggacaa gaaccgcatc ggactggcga tggaagtggc
     1321 tgtgccgccg ctggcgttcc tcggactgat gtggacggtt gcggcaggtt tgtcgctcgc
     1381 tctgtatgtg ttctacggcc aaggcggacc gcttggcttt ctggtgggta tggcagcgat
     1441 atttgctgtg gccgtgctga tgtcctggat gcggtttgcc ggaatcaaat cgacgctggc
     1501 ggcgctggcc tcggtgccgg gttatctcat gtggaagctg cccatgtacc gcgaattttt
     1561 cactcgccgc gaaacgcggt ggatgaagac agcgcgcgac agcctcaacg gcgcccaaca
     1621 cggattacgt gcgccatttc ctgggtaagt tcggcagaaa cgaaatgggt cagcctttct
     1681 tctgtggcgg gcctgtatcc aggagctgtc ggataatgca catcgagatt gaagcagcac
     1741 tcgttcccgc cacggtgcgg ctgtttggat ccgggttgct gggattgctc ggcgctgtgc
     1801 ggcgtcggaa gtttgcaaat cacgaaagag ttttatccgt tccttagcaa gcttggcggg
     1861 cggcgcaagc cgcccgcatt ttctttgtaa ggtagggagc ggcgttaatt atagatcagc
     1921 ccacggtctg gttactcggc cccagcaggt tccgcgcgat ccgtcacggt tttcgtgcca
     1981 cctgtatctc ccgtctctta acccatttcg ggtcattggt tggcgtctcc gatcaaatca
     2041 tctattctct gcggcaacaa tattcctgac agggaagcag catgatcaac attgccgtca
     2101 ccggcgccgc cgggcgcatg ggccgcacgc tcatcgaggc gtgcgcgcgc gccgaaggcc
     2161 tgcgtctgag tgcagctatg gagcgagccg ggaacactgc ggtgggcaag gacgcgggtg
     2221 aagtggccgg agtgggcaag cttggcgtca ctgttcgttc cggtttggat ggagcggatt
     2281 tcagcgtgct ggtggatttc accagcccgg aagcgacgct cgcgaatctc gattactgcc
     2341 gccagaatgg aaagaaaatg gttatcggta ctaccggcct ggatgacgcc gggaaaagtc
     2401 gtatcgctgc cgccggcaag gacatcgcca ttgtatttgc gcccaacatg agcgtgggcg
     2461 tgaatctgtg tttcaagctg ctcgaaaccg ccgcgcgcgt gctgggcgat gacgtggacg
     2521 tggagattgt cgaagcgcat caccgccaca aggccgacgc accctcggga acggcgctgc
     2581 gcatgggcga ggtgatcgcc agggtgttgc agcgggacct caaggaacat ggcgtttacg
     2641 gccggcacgg catcagtggc gaacgcgcgc gcaagaccat tggtttctcc agcatccgcg
     2701 ccggcgacat cgtcggcgat cacactgtgc tgtttgccgg cggcggcgag cgcgtggaga
     2761 tcacgccccg tgccgaatcg cgcatgccct tcgccaacgg agcgc
//
LOCUS       DBNGZE010000447         2340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1311986, whole genome shotgun sequence.
ACCESSION   DBNGZE010000447 DBNGZE010000000
VERSION     DBNGZE010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1311986"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="trmFO"
                     /locus_tag="AC16FV_11670"
     CDS             <1..1071
                     /gene="trmFO"
                     /locus_tag="AC16FV_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR2850743.1"
                     /translation="LITLERGEVALPPEDWDSVIIATGPLTSPDLAATIGRLTGEQQL
                     AFFDAIAPIVHRESLDLTQAWFQSRYDKPGPGGSGADYINCPLSREQYETFVAALLAA
                     DKTSFHEWEESTPYFNACLPIEVMAERGPETLRHGPMKPFGLTNPHDPGKTPYAVVQL
                     RQDNKLGTLFNMVGFQTKLKHGEQIRIFRTIPGLQNAEFARLGGLHCNTFLNSPKLLD
                     ETLRMKANPRWRFAGQITGCEGYVESAAVGLLAARFAAAGRRGERIVPPPATTAHGAL
                     LAHITGGHIETIDMGTSSFQPMNINFGLFPPLPEAPTFDRYSKLGRSTAKALARKSAL
                     TRRALADLDRWIAGGLPAVAAE"
     gene            complement(1075..1920)
                     /locus_tag="AC16FV_11675"
     CDS             complement(1075..1920)
                     /locus_tag="AC16FV_11675"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR2850744.1"
                     /translation="MQTHFEHCARLVREADRERYLATLFAPAGYRNALFALYAFNVEI
                     SRVRDLAREAMPGEIRLQWWREILLGERAEEAASNPTAAALLETLGQDNSERLIALID
                     AHIFDLYNEPMPAISDLQSYAVATAGSIFELAARILGGDTGLEVAALAAEAGQAQTIA
                     NVLAQLPRHAARRQLYVPVEVLQHYRAEPDDIFALRATPEVRAALAELRLRARHYLAR
                     VGAAGTEIPNNMQPAFLPLAPLWQWLRDMERPDYDPFRPPQVAPWRRQWRIWRAAKSF
                     SRVGA"
     gene            complement(1940..2329)
                     /locus_tag="AC16FV_11680"
     CDS             complement(1940..2329)
                     /locus_tag="AC16FV_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR2850745.1"
                     /translation="MVLGGHADAPHLPRPARIDAYGDGGFRFAGMSHRGSLLCFPNGI
                     WAWPVNNVAELDADTLSAAFKRAESLDFFLIGTGRESWVLPAHLRDRFRELSLSVDEM
                     ATGAAVRTYNILLAENRRIGAGLIAVA"
BASE COUNT          523 a          724 c          653 g          440 t
ORIGIN      
        1 ttaatcacac ttgaacgcgg cgaagttgct ctaccccccg aggactggga tagcgtgatc
       61 atcgcgaccg gtccccttac ctcgcccgat cttgccgcga caattggcag gctgaccggt
      121 gaacagcagc tcgctttctt cgacgctatc gcgccgattg tgcatcgcga gagcctcgac
      181 cttacgcagg cgtggttcca gtcgcgctac gacaaacccg gccctggcgg ctctggcgcc
      241 gattacatca actgtccgct ttcgcgcgaa caatatgaga ctttcgttgc cgctttgctg
      301 gccgccgata aaaccagctt tcacgagtgg gaagagtcaa cgccctattt caacgcttgc
      361 ctgccgatcg aagtcatggc cgagcgtggc cccgagacat tgcggcatgg cccgatgaag
      421 cccttcggcc tgaccaatcc gcacgatccg ggcaaaacgc cctatgcggt ggtgcagcta
      481 cggcaagaca acaagctagg caccttgttc aacatggtcg gcttccagac aaagctaaaa
      541 cacggtgagc aaattcgtat ttttcggacc attccaggtt tgcagaacgc ggaatttgcc
      601 aggcttggcg gcctgcactg caatactttt ctcaattcgc caaagttgct tgatgaaaca
      661 ctgcgaatga aagcaaaccc acgctggcgt tttgccggtc agatcaccgg ctgcgaaggc
      721 tatgtggaat ctgcggccgt cggtttgctc gcggcgcgct ttgccgccgc cgggcggcgc
      781 ggggagcgga tcgtgcctcc acccgcgacc acagcgcatg gcgcactgct cgcgcatatt
      841 accgggggcc acatcgaaac gatcgatatg gggacgtcct cattccagcc aatgaacata
      901 aattttggct tgttcccgcc gttacccgag gctccgacat tcgaccgata tagcaagctg
      961 ggacgtagca ccgccaaagc gctcgcgcgc aagagcgccc tgaccaggcg cgcgctcgcc
     1021 gatctcgacc gctggattgc tggaggtttg ccggctgtgg cggccgagta atcgctaggc
     1081 accaacccga ctaaaggact tcgccgcgcg ccagatgcgc cactgccgcc gccacggtgc
     1141 cacttgggga ggccgaaacg ggtcatagtc aggccgctcc atatcgcgta gccattgcca
     1201 cagcggcgcc aaaggcaaga aggcgggctg catattattt ggaatttcag tgccggcggc
     1261 tccgacccgc gcgagataat gtcttgcgcg caagcgaagt tcagcgagtg cagcgcgcac
     1321 ttcaggtgta gcgcgcaaag cgaaaatatc gtcaggctct gcgcgataat gctgaaggac
     1381 ctcgacaggc acataaagct gacggcgcgc cgcatggcgc ggtaactgag ccagcacgtt
     1441 tgcaatcgtt tgcgcctggc cggcctccgc ggcgagcgcg gcaacctcca aaccggtatc
     1501 accgccgagg atgcgtgcgg ccaattcgaa tatcgaacca gcggttgcca ccgcgtagga
     1561 ctgaagatca gagatagctg gcattggctc gttgtagaga tcaaagatat gcgcatcgat
     1621 caaggctatt aggcgctcac tattgtcctg ccctagtgtt tccaacaatg ccgccgcagt
     1681 tggattcgac gcagcctcct ccgcacgctc tcccaagaga atttcgcgcc accattgcaa
     1741 ccggatttcg cctggcattg cctcgcgcgc caaatcgcgc acgcgcgaaa tctccacatt
     1801 gaatgcgtaa agtgcaaaca aggcattgcg gtaaccggcg ggcgcgaaca acgtggccag
     1861 atagcgttcc ctatcagcct ctcgaacaag cctggcacaa tgctcgaaat gggtttgcat
     1921 cgggcaagac tagcgcgaat caagcgactg caatcaatcc cgcaccaatg cgccggtttt
     1981 cagccaaaag aatattgtaa gttcgcactg ccgcgcctgt cgccatttca tcaacggata
     2041 gcgacaattc acgaaaacga tcccgcaagt gtgccggtaa cacccaggac tcacggcccg
     2101 tgccgatcag gaaaaagtcg agactttccg cacgcttaaa agcggccgac agcgtatccg
     2161 catccagttc ggccacgtta ttaacaggcc aggcccaaat gccgttcgga aaacatagca
     2221 gcgagccccg atgcgacatt ccggcgaaac ggaagccacc atcgccgtaa gcgtcgatac
     2281 gcgcagggcg tggcagatgc ggagcatcgg cgtggccgcc gagcaccatc tatttccgat
//
LOCUS       DBNGZE010000448         3504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_965972, whole genome shotgun sequence.
ACCESSION   DBNGZE010000448 DBNGZE010000000
VERSION     DBNGZE010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_965972"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /gene="gltX"
                     /locus_tag="AC16FV_11685"
     CDS             complement(<1..1184)
                     /gene="gltX"
                     /locus_tag="AC16FV_11685"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR2850746.1"
                     /translation="MTARPFKTRFAPSPTGLLHLGNIRTALFNFLLARQAQGTFLLRL
                     EDTDAMRGHEKYARALEDDLHWLSLAWNEGPQAGGHHWPYAQSERGSIYKEYFTALES
                     KGLAYPCFCSEHELAIARKTALAAHRPPRYSGKCRALSSQEVQARFARGEPATLRFRV
                     EEGKTVEFEDKVRGRQVFSTDDIGDYVIRRSDGTPAFFFCNAIDDALMGVTLVVRGED
                     HLTNTPRQILLLQALGLPLPEYAHIALVVGADGAPLSKRTGSKSVEELRSVGYLPSAI
                     NNYLARLGHTYDANAFMSLEVLAENFDLSRLGRAPARYDEAQLMHWQREAVLHLPSAA
                     LREWMGEAVQALVPADSFTAFIEAVRGNISFPADALRWAQIVFTDKLVVSHAAREAMT
                     AAG"
     gene            1329..1829
                     /locus_tag="AC16FV_11690"
     CDS             1329..1829
                     /locus_tag="AC16FV_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2850747.1"
                     /translation="MIHMKTNYGTIVLELNPGKAPKTVENFLAYAKDGFYDATLFHRV
                     IDKFVIQGGGFGTGMTQKMTRPPIENEANNGLRNARGTLAMARATDPHSATSQFFINI
                     VDNDFLNYTAPTPAGWGYCVFGKVIDGMHIVDQIKGVATGSKLGHKDVPLSDVLIEKM
                     QIIDEK"
     gene            1846..2613
                     /locus_tag="AC16FV_11695"
     CDS             1846..2613
                     /locus_tag="AC16FV_11695"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR2850748.1"
                     /translation="MPATTLFISDLHLCGTRPAITRLFLDFLRRRARAADALYILGDL
                     FEYWIGDEAIEQEEFRAIVHGLRELTAAGTPVFVMHGNRDFLMADGFEKASGCRLLAD
                     PARVDLHGIPTLLMHGDSLCIDDIEYMAFRVQVRNPAWQREFLAKSVAERDRIVRDFR
                     EISKNSTASKKPEIMDVNQNAVEAIMREYGVRRLIHGHTHRPKEHVFTLDGQSARRMV
                     LGDWYEQGSVLSVDARGWTLEGLPLKSKNVGRVSADT"
     gene            complement(2703..3410)
                     /locus_tag="AC16FV_11700"
     CDS             complement(2703..3410)
                     /locus_tag="AC16FV_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850749.1"
                     /translation="MRRVAYLLACLWPLMLISCSEPTTPAAVLYFVEHEPGANPDRTR
                     MIITAGFLRMDGGADDVDFLLFDRADGTIYSVSGAERQILVIPPRPMDAKAPPNLTHK
                     VIRDDAAYPAVGSHKVSHYELLTNGKRCYDLYAAAELMPEAVAALRQYREVLAGQQAK
                     TLSMTPAEMQSVCDLANNVFLPARHLEHGFPVRLTDMTGKTIELVDYNTEFRATAAVL
                     RLPADYKRLTIEELRGK"
BASE COUNT          717 a         1121 c         1041 g          625 t
ORIGIN      
        1 ccggccgccg tcatggcctc ccgtgccgca tggctgacga caagcttatc cgtaaacacg
       61 atctgcgccc agcgcaaggc gtccgcggga aaagaaatat ttccccgcac cgcttcaatg
      121 aacgccgtaa agctgtcggc aggcacaagc gcctgcaccg cctcgcccat ccattcccgc
      181 agcgccgcgg acggcaggtg caacaccgcc tcgcgctgcc agtgcatcag ttgcgcctcg
      241 tcataacgcg ccggcgcccg acccagccgg gacaggtcga aattttccgc caggacttcc
      301 aggctcataa aggcattggc atcgtaggta tgtcccagac gcgccaggta attattgatg
      361 gcggacggca gataacccac ggagcgaagc tcctcgaccg atttcgaacc ggtacgcttc
      421 gagagcggcg cgccgtcggc gccgaccacg agcgcgatat gcgcgtactc cggcagcggc
      481 agcccgagcg cctgaagcag caggatctgg cgcggtgtgt tagtcagatg atcctcgccg
      541 cgcacgacca gcgtcactcc catgagggcg tcgtcgatgg cgttgcagaa gaaaaatgcc
      601 ggcgtgccgt ccgaacggcg gatgacgtag tcgccgatgt catccgtgct gaacacctgc
      661 cgcccgcgca ccttgtcttc gaactcgacg gttttcccct cttccacccg aaaccgcaac
      721 gtcgcgggct cgccgcgggc gaagcgggcc tgcacctcct gcgacgacaa tgcgcgacat
      781 ttgccggagt aacgcggcgg gcgatgcgcc gccagcgccg tcttgcgcgc gatcgccagt
      841 tcatgctcgc tgcagaaaca gggataggcc agacccttgg attcgagggc agtgaagtat
      901 tccttgtaga tcgaaccgcg ctcggactgc gcatagggcc agtgatggcc gccggcctgc
      961 ggtccctcgt tccaagcgag actcagccaa tgcaggtcgt cttccagcgc gcgggcgtat
     1021 ttttcgtgcc cgcgcatggc gtcggtgtct tccaggcgca gcaagaaggt tccctgcgcc
     1081 tgccgggcca gcagaaaatt gaaaagcgcc gtgcggatat tgccgaggtg cagcagaccc
     1141 gtggggctgg gggcaaagcg ggtcttgaag gggcgcgcgg tcatcgtgaa gggcgccatg
     1201 ttagcggaaa ccgccctccc ggcgaagtcc cggctttacg acaggaatag caatccagtg
     1261 taaacttcaa tacaagaccc gccgccggcg ggttcactca gccatcagaa cacaagagcg
     1321 cccatttaat gattcacatg aaaaccaact acggcaccat cgtcctggag ctcaatccgg
     1381 gcaaggcgcc gaagaccgtg gagaactttc tcgcctacgc gaaagacggc ttttatgacg
     1441 ccacgctgtt tcaccgcgtg atcgacaaat ttgtcatcca gggcggtggt ttcggcacgg
     1501 gcatgacgca gaagatgaca cggcctccca tcgagaatga agcgaacaac ggtttgcgca
     1561 acgcccgcgg cacgctcgcc atggcgcgcg ccaccgaccc gcactccgcc accagccagt
     1621 tcttcatcaa catcgtggac aacgatttcc tgaattacac cgcgcccacg ccggcgggct
     1681 ggggttattg cgtgttcggc aaggtcatcg atggcatgca catcgtcgac cagatcaagg
     1741 gagttgccac cggctccaag ctcggtcaca aggacgtacc gctgtccgac gtgctgatcg
     1801 agaaaatgca gatcattgac gagaagtagg agacccagcg aagagatgcc ggccaccacg
     1861 ctcttcatct ccgacctgca tctctgcggc acccgccccg ccatcacccg gctgttcctc
     1921 gacttcctcc ggcgtcgcgc gcgcgccgct gacgcgctct acatcctcgg cgatctgttc
     1981 gagtactgga tcggcgacga ggccatcgag caggaggaat tccgtgccat cgtccacggc
     2041 ctgcgtgaat tgacggcggc tggcaccccg gttttcgtca tgcacggcaa ccgcgatttt
     2101 ctgatggcgg acggtttcga aaaggccagc ggttgccgcc tgctggccga cccggcgcgc
     2161 gtcgatctcc acggcatacc caccctgctg atgcatggcg attcgctgtg catcgacgac
     2221 attgaataca tggcgttccg cgtccaggtg cgcaatcccg cctggcagcg ggagttcctg
     2281 gccaagtccg tggccgagcg cgaccgcatc gtgcgcgact tccgcgagat cagcaaaaat
     2341 tcaaccgcca gcaagaaacc ggaaatcatg gatgtgaacc agaacgcggt ggaagcgatc
     2401 atgcgcgagt atggcgtgcg tcgtctgatc cacggccaca cacaccgtcc gaaggaacac
     2461 gtgttcacgc tcgacggcca atccgcacgg cgcatggtgc tcggtgactg gtacgaacag
     2521 ggcagcgtgc tgagcgtaga tgcgcgcggc tggacgctgg aaggtttgcc actgaaatct
     2581 aaaaatgtag ggcgggttag cgctgatacg tagcgcgtaa cccgccgaac ccggcgaagt
     2641 tcaccgtaat cacgccacga ccgtgggtta cggcaaacac cacctaaccc acgctacatt
     2701 tattatttcc cgcgcagctc ctcgatggtc aggcgcttgt aatccgccgg caggcgcagc
     2761 accgcggccg tggcgcgaaa ctccgtgttg taatccacca gttcaatcgt cttgccggtc
     2821 atgtccgtga gccggaccgg aaaaccatgc tccagatggc gcgccggcag aaacacgtta
     2881 ttcgccagat cgcagacaga ctgcatttcc gcgggggtca tcgacagggt tttggcctgc
     2941 tgcccggcca gcacctcacg gtattggcgc aaggccgcca ctgcctccgg catcagctcc
     3001 gccgccgcat agaggtcgta acagcgtttt ccgttggtca gcaactcata atggctgact
     3061 ttgtgactcc cgacggcggg atatgcagcg tcgtcgcgaa tcaccttgtg tgtcagattg
     3121 ggtggagctt tggcgtccat cggtcgcggt gggatgacca gaatctgccg ctccgcgccg
     3181 gaaacgctgt aaatggtgcc gtcggcccga tcgaacagca gaaagtccac gtcatcggcg
     3241 ccgccgtcca tgcgcaggaa acccgcggta atgatcatgc gcgtgcgatc gggattggcg
     3301 cccggctcgt gttcgacgaa atacagcaca gcagccggcg ttgttggctc ggaacaggag
     3361 atcaacatca atggccacaa gcaggccagc agatacgcaa cgcgtcgcat ctcttcgttc
     3421 tcaaacccaa catgatgtcc atgacattgg tgccggtcgg accggtttga atcaggtcgc
     3481 cgctcgcttc cagaaaagtc ccgg
//
LOCUS       DBNGZE010000449         4726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1937359, whole genome shotgun sequence.
ACCESSION   DBNGZE010000449 DBNGZE010000000
VERSION     DBNGZE010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1937359"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="AC16FV_11705"
     CDS             <1..286
                     /locus_tag="AC16FV_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2850750.1"
                     /translation="KTYLVRWASLAVLILGAAITGLSALPGTAALILWGALSVLAVLH
                     EIIGRALFYVLVTPTTMPGAFFWNNKYFEAHARQTGLARLPQTGVVADAH"
     gene            complement(403..2499)
                     /gene="pnp"
                     /locus_tag="AC16FV_11710"
     CDS             complement(403..2499)
                     /gene="pnp"
                     /locus_tag="AC16FV_11710"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR2850751.1"
                     /translation="MAKITKSFQYGKHTVTLETGEIARQASGACVASMGETVVLATVV
                     AMKEPMPGKDFFPMTVDYQEKTFAAGRIPGGFFKREGRPSEKEILTSRLIDRPIRPLF
                     PEGFTNEVQIIATVISVDPDIDADIVAMLGASAALKLSGAPFNGPIGATRVGYQNGQY
                     LLNPGNKELVDSKLNLVVAGTAKAVLMVESEAQELSEEVMLGAVVFGHEQMQVAIKAI
                     NDLVEEAGVQPWAWQPPVEDTSLKNTLQTEFGPLIAEGYRIKEKTARQDRMVEIREMA
                     VGKHAAEGAPEGTKEKVLKIIGDIEYRTVRDKILSGEPRIDGRDTRTVRPITIRTGVL
                     PRTHGSALFTRGETQALVVTTLGTGRDAQIIDALEGERKDPFMLHYNFPPYSVGEVGR
                     VGSPKRREVGHGRLAKRAVVAVLPDMATYPYVLRVVSEITESNGSSSMATVCGASLSL
                     MDAGVPIKAPVAGVAMGLIKEEKRFAVLTDIIGDEDHLGDMDFKVAGTTGGVTALQMD
                     IKIEGINKEIMDVALKQAKEGRLHILGEMSKAISAPRAELSEYAPRIIAIKINPERIR
                     DVIGKGGATIRALTEETGCTIDIEDDGTVKIASADNIAAQEAVRRVKQLTADVEVGMV
                     YEGKVAKLMDFGAFVTILPGKDGLVHISQISRERVERVGDKLREGEVVRVKVLEVDKQ
                     GRIRLSIKALEEERIS"
     gene            complement(2543..2812)
                     /gene="rpsO"
                     /locus_tag="AC16FV_11715"
     CDS             complement(2543..2812)
                     /gene="rpsO"
                     /locus_tag="AC16FV_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR2850752.1"
                     /translation="MTVPRETKAKVMQEYQTHKGDTGSPEVQVALLSTQIEELSAHFQ
                     THPKDHHSRIGLLKMVNKRRQLLEYLKKADSDRYRALIERLGLRK"
     gene            complement(2941..3816)
                     /gene="truB"
                     /locus_tag="AC16FV_11720"
     CDS             complement(2941..3816)
                     /gene="truB"
                     /locus_tag="AC16FV_11720"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR2850753.1"
                     /translation="MLLDKPVGLTSNQALQTVKHLLNACKAGHSGSLDPIATGLLPLF
                     FGEATKLTQFLLNADKRYWTVFRLGQSTTTYDSEGEVTATRPVNAGRRDIERALERFR
                     GEIEQVPPMYSAIKHQGEALYKLARAGVEIEREPRQVTIHEIKLLGLENDLLTLEIAC
                     SKGTYVRSLAHDLGEALGCGAHVVQLRRTGIGRVGIENAVPLEHLQALGSPAECARLL
                     QPADSVLQELPGVHLTPLAAHYLKQGQAVSMQHGFRPGWVRLYEGDKRFLGMGQVQDD
                     GRVAPRRLLALGENP"
     gene            complement(3858..4226)
                     /gene="rbfA"
                     /locus_tag="AC16FV_11725"
     CDS             complement(3858..4226)
                     /gene="rbfA"
                     /locus_tag="AC16FV_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR2850754.1"
                     /translation="MKTESNRPRRVAELIRRELAMIIPRELDDPHAHQITITGAEVSR
                     DMSSARIYFSLLSGVAEAKQTTKSLNRAAGFLRHALMGRVSLRTVPALRFYFDESVER
                     GARIDSLIEQAIAEDKTHKE"
     gene            complement(4309..>4726)
                     /locus_tag="AC16FV_11730"
     CDS             complement(4309..>4726)
                     /locus_tag="AC16FV_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HIR2850755.1"
                     /translation="SSARKLIEHQGVDVHYYNVIYDAVEEIKAAMTGMLKPQIKEQML
                     GLAEVRDVFRSPKMGAVAGCYVVEGVVKRNRPIRVLRDNVVIFEGELESLRRFKEDAG
                     EVKAGLECGIAVKNYNDVKIGDQIEVFERVEVARTL"
BASE COUNT          814 a         1471 c         1456 g          985 t
ORIGIN      
        1 caaaacctat ctcgttcgct gggcaagtct tgccgtcctg attctcgggg ccgcgattac
       61 cggcctatcc gcgctgccgg gcacagcggc gctgattctc tggggtgcgc tgtcggtcct
      121 ggccgtcctg catgaaatca tcggacgcgc gctgttttac gtgctggtca ctccgacgac
      181 catgcccgga gcgttcttct ggaataacaa atatttcgaa gcccacgcgc gccagaccgg
      241 cctcgcccgc ctgccgcaga ccggggttgt cgccgatgcg cattaatatt ctccagcaaa
      301 aatagggcgt gtagtacacg tcctactcct gctgcctaca tctaacggac acaaaaaaag
      361 ggagccgaag ctcccttttt taactgccta tgcgcgaaac actcaagaaa tccgctcttc
      421 ttccagcgcc ttgatggaga ggcggatgcg gccctgcttg tccacttcca gcaccttgac
      481 gcggaccacc tcgccctcgc gcagtttgtc gccgacgcgc tcgacgcgct cgcgcgagat
      541 ctgcgagatg tgtaccaggc cgtccttgcc gggcaggatg gtgacgaagg cgccgaaatc
      601 catgagcttg gcgaccttgc cttcgtagac catgccgacc tcgacatcag cggtgagctg
      661 cttcacgcgg cgcacggctt cctgcgcggc gatgttgtcg gccgaggcga tcttgaccgt
      721 gccgtcgtcc tcgatgtcga tggtgcagcc agtctcctcg gtgagcgcgc ggatggtggc
      781 gccgcccttg ccgatgacgt cgcggatgcg ctccgggttg atcttgatgg cgatgatgcg
      841 cggcgcgtac tcggacagct cggcgcgcgg tgcggagatc gccttgctca tctcgcccag
      901 gatgtgcagc cggccttcct tcgcctgctt gagcgcgaca tccatgattt ccttgttgat
      961 gccttcgatc ttgatgtcca tctgcagcgc ggtcacgccg ccggtggtac cggcgacctt
     1021 gaagtccatg tcgccgagat gatcctcgtc accgatgatg tcagtgagca cggcgaagcg
     1081 tttttcttcc ttgatcaggc ccatcgccac acccgcgacc ggggccttga tgggcacgcc
     1141 ggcatccatc agcgacagcg aggcgccgca gacggtggcc atggagctgg agccgttgga
     1201 ttcggtgatc tcggatacca cgcgcaggac ataaggatag gttgccatgt ccggcaacac
     1261 cgccacgacg gcgcgcttgg ccagacgacc gtgaccgacc tcgcggcgct tggggctgcc
     1321 gacgcggccg acttcaccca ccgaatacgg cgggaagttg taatgcagca taaaagggtc
     1381 tttgcgctca ccttccagag cgtcgatgat ctgcgcgtcg cgcccggtgc cgagcgtggt
     1441 gacgaccagc gcctgggtct caccgcgcgt gaacagcgcc gagccatggg tgcgcggcag
     1501 cacgccggtg cggatggtga tcggacgcac ggtacgcgtg tcgcggccgt cgatacgcgg
     1561 ctcaccggag agaatcttgt cgcgcacggt gcggtattcg atgtcgccga tgatcttgag
     1621 gactttttcc ttcgtgcctt cgggcgcgcc ttcggcggcg tgcttgccga ccgccatctc
     1681 gcggatctcg accatgcgat cctgacgcgc ggtcttttct ttgatgcgat agccctcggc
     1741 gatgagcgga ccgaattcgg tctgcaaggt gttcttgagc gaggtgtcct cgaccggcgg
     1801 ctgccaggcc cagggctgta caccggcctc ttccacgaga tcattgatgg ctttgatcgc
     1861 cacctgcatc tgctcgtggc cgaacacgac cgcgccgagc atgacctctt ccgacaattc
     1921 ctgcgcctca gactcgacca tcaacacggc tttcgcggta ccggcgacga cgaggttgag
     1981 cttcgaatcg acgagttcct tgttgcccgg attaagcagg tactggccgt tctggtagcc
     2041 gacgcgcgtg gcaccgatgg ggccgttgaa cggcgcgccc gaaagcttga gcgccgcgga
     2101 ggcgccgagc atggcgacga tatccgcatc gatatccggg tccaccgaga tgacggtggc
     2161 gatgatctgg acttcgttgg tgaaaccttc ggggaacagc gggcggatgg gacggtcgat
     2221 gaggcgcgag gtgaggattt ctttttccga gggacggcct tcacgcttga agaatccgcc
     2281 cgggatgcgg ccggcggcga aggttttttc ctgatagtcc acggtcatgg ggaaaaaatc
     2341 cttgccgggc atcggttcct tcatcgccac cacggtggcg aggacgacgg tttcgcccat
     2401 gctggcgacg caggcaccgc tggcctgacg cgcgatctcg ccggtttcca gggtgacggt
     2461 atgtttgccg tactgaaatg atttcgtgat ttttgccaag accttgtcct cgaattactt
     2521 cgttactgac tgattaaact gcttacttgc gcaggccgag ccgctcgatg agcgcgcggt
     2581 aacggtcgct gtcggccttc ttcaggtatt cgagcagctg acggcgcttg ttcaccatct
     2641 tcagaagacc gatgcgcgag tgatgatcct tcggatgggt ctgaaagtgg gcggataatt
     2701 cttcgatttg cgtggacagc agggcaacct ggacctcggg cgagccggtg tcacctttgt
     2761 gagtctggta ttcctgcatg accttggcct tggtctcgcg ggggacggtc atcaatattt
     2821 acctcaattt tctgtcagtt acgctttgga gggcgcgtat tctacctgcg gggggacccc
     2881 gcctcaaccg ccaatatcgg gtccgaacac gccgtacgga tcgccggttt catgcctgtt
     2941 tcagggattt tcgcccagcg ccagcaggcg ccgcggcgcc acgcgcccgt catcctgcac
     3001 ctgccccatg ccgagaaagc gcttgtcgcc ttcgtacagc cgcacccaac cgggcctgaa
     3061 gccatgctgc atcgacaccg cttgcccctg tttcaggtaa tgggccgcga gtggcgtcag
     3121 gtgcacaccc ggcagctcct gcagcacgct gtccgccggt tgcaacaacc gcgcgcattc
     3181 cgccgggctc ccgagggcct gcaggtgctc gagcggaacg gcgttttcga tgccgacacg
     3241 gccaatgccg gtacggcgta actgcaccac atgcgcgccg caacccaggg cctcgcccag
     3301 gtcatgcgcc aggctgcgca cataggtacc cttggagcag gcaatttcca gcgtcagcag
     3361 gtcattttcg agtcccagca gcttgatctc gtggatggtt acctggcgcg gctcgcgctc
     3421 gatctcgacg ccggcgcggg ccagcttgta gagcgcctcg ccttgatgct tgatggcgga
     3481 atacatcggc gggacctgct cgatctcgcc gcgaaaacgt tccagcgcgc gttcgatgtc
     3541 gcgtcggccg gcgttgaccg ggcgcgtggc cgtgacctcg ccttcgctgt cgtaggtggt
     3601 ggtgctctgc cccagccgga acaccgtcca gtagcgcttg tcggcgttga gcagaaactg
     3661 cgtcagcttg gtggcctcgc cgaaaaacag cggcaacaaa ccggtggcga tggggtcgag
     3721 gctgcccgag tgtccggcct tgcaggcgtt gagcaggtgt ttgaccgtct gcaacgcctg
     3781 gttcgaggtc agccccaccg gcttgtcgag caacatcact ccattgagcg gagtacagtg
     3841 acggcgtccg cgcacgctta ctccttgtgg gtcttgtctt cagcgatagc ctgttcgatc
     3901 aggctgtcga tgcgcgcgcc gcgttccacc gattcatcga aataaaaccg cagtgccggc
     3961 acggtacgca acgacaccct tcccatgagg gcatgccgca ggaaccccgc ggcgcggttg
     4021 agcgacttgg tcgtctgctt cgcctccgcc actccgctca gcagcgagaa atagatgcgc
     4081 gcggaagaca tgtcgcgcga cacttccgcg cccgtgatgg tgatctggtg cgcgtgggga
     4141 tcgtcgagct cgcgcgggat gatcatggcg agttcgcgcc ggatgagttc agcgacacgc
     4201 cgcggacggt tggattctgt tttcataaag aatatggaac cgcagatgaa cgcggataat
     4261 tacaaataaa cggaagattt atctgcgttt atccacgtta caaggacgtt acaaggtccg
     4321 ggcgacctcg acacgctcga agacctcgat ctggtcgccg atcttcacat cgttgtaatt
     4381 cttcacggcg atgccgcatt cgagcccggc cttcacttcg cccgcgtctt ccttgaaacg
     4441 gcgcagggat tcgagctcgc cctcgaagat aacgacatta tcgcgcagca cgcggatcgg
     4501 gcggttgcgt ttcaccacgc cttcgaccac gtagcagccg gcgaccgcac ccatcttggg
     4561 cgaacggaac acgtcgcgta cctcggccag tcccagcatc tgctccttga tctggggctt
     4621 gagcatgccg gtcatggcgg ccttgatctc ttccaccgcg tcgtagatga cgttgtagta
     4681 atgcacgtcg acgccctgat gctcgatgag cttgcgcgcg gaactg
//
LOCUS       DBNGZE010000450         2499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_272159, whole genome shotgun sequence.
ACCESSION   DBNGZE010000450 DBNGZE010000000
VERSION     DBNGZE010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_272159"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="AC16FV_11735"
     CDS             complement(<1..1422)
                     /locus_tag="AC16FV_11735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR2850756.1"
                     /translation="MTITRRLMLKALSAGVLGAVTAPALLLPREVRAAVTPFSLPLRI
                     PPVLAPATSTSTADYYDLQIREAYAEIIPGQQTRIIGFNGMTPGPTIRARAGRQTVMS
                     HFNGLPPTTLWNTPGNCTIHLHGGHIPASEDGYPTDYIRPGTTRSYTYPNNQLPTTLW
                     YHDHCMDFTGPHVWYGMAGLYLLSDDYEDSLGLPNGAYEVPLVLQDRNFDTSGQLVYT
                     RNRMQGETGNTMLVNGTIQPYFNVATRRYRFRVLNGSNARFYRLALSNGASFRVLGME
                     GGLLPAPVTVTSLTLAPAERADIVIDFGSLAVGTKVELRNTLVSSTNSVYKVMSFNVN
                     RQETDPSKVPATLRPITKLNPAQAVITRDFNISMMMGMGNPMWVINGLGFDANRIDAF
                     PKLGTTEIWRFTNRSGMAHPIHMHDVQFQILDINGVAPAATHAGWKDVVIVPAMGSAR
                     IVAKFEDYTGLYMFHCHMLEHEDY"
     gene            complement(1556..1939)
                     /locus_tag="AC16FV_11740"
     CDS             complement(1556..1939)
                     /locus_tag="AC16FV_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850757.1"
                     /translation="MLSALLLFLTTAGTCSAFPEFACCPDRANHAQPEHAHAGSGQGA
                     DHDHAVAPLSHSDSGVSVADSPDCDPGNCECCQSFFLAHLSAIPVAITVPIFPDCSVD
                     HPALCRMALEFASAPPVPPPQPYLV"
BASE COUNT          444 a          724 c          827 g          504 t
ORIGIN      
        1 gtagtcctcg tgctcgagca tgtggcagtg gaacatgtac aggccggtgt agtcctcgaa
       61 cttggcgacg atgcgcgcac ttcccatcgc cggcacgatc accacgtcct tccagcccgc
      121 gtgcgtcgcc gccggcgcca ccccgttgat gtcgaggatc tggaactgca cgtcgtgcat
      181 atggatcgga tgagccatgc ccgagcggtt ggtgaagcgc cagatctcgg tggtgccgag
      241 cttggggaag gcatcgatgc ggttggcgtc gaagcccaag ccgttgatga cccacatcgg
      301 attacccatg cccatcatca tgctgatgtt gaagtcgcgg gtgattaccg cctgcgccgg
      361 gttgagcttg gtgatcggcc gcagggtcgc cggcaccttg cttgggtcgg tttcctggcg
      421 gttgacgtta aagctcatca ccttgtagac gctgttcgtg ctgctcacca gcgtatttcg
      481 cagctccacc ttcgtgccca ccgcgagact gccgaagtcg atcacgatgt cggcgcgttc
      541 cgccggtgcc agcgtcagcg aggtcaccgt caccggcgct ggcagcaggc cgccctccat
      601 gcccagcacc cggaacgacg caccattgct gagggccagc cgatagaagc gggcgttcga
      661 gccgttgagc acgcggaagc ggtagcggcg cgtggcgacg ttgaagtacg gctggatggt
      721 gccgttcacg agcatggtgt taccggtctc gccctgcatg cggttgcgcg tgtagacgag
      781 ctggccactg gtatcgaagt tacggtcctg aagcaccagc ggcacctcat aggctccgtt
      841 gggcagaccc agactgtcct cgtaatcatc cgacagcaga taaagacccg ccatcccgta
      901 ccacacgtgc ggcccggtga agtccatgca gtggtcgtga taccacaacg tggtcggcag
      961 ctggttattg gggtaggtgt agctgcgtgt cgtgccgggg cggatgtagt ccgtggggta
     1021 gccatcctcg gacgcgggga tgtgcccgcc atgcagatgg atggtgcagt taccgggtgt
     1081 gttccacagg gtggtgggcg gcaggccgtt gaagtggctc atcaccgtct ggcgcccagc
     1141 gcgcgcgcgg atcgtcggcc ccggcgtcat gccgttgaag ccgatgatgc gcgtctgctg
     1201 gcccgggatg atttccgcgt aggcctcgcg gatctgcagg tcgtagtagt cagcggtgct
     1261 ggtgctcgtg gcgggcgcga gcaccggcgg gatgcgcagt ggcagcgaga acggcgttac
     1321 cgccgcccgt acctcgcgtg gcaggagcag agcgggtgca gtgacggcac ccaatacgcc
     1381 cgcgctcagc gctttcaaca tcagtcgtct agtaatagtc atgatcattt ctccgcagtg
     1441 gtaatagtct ggacctctgt ccgcgcccct ccgtactgtt cgggcgcgaa cacaaccgtg
     1501 tgcgacgcct catcaagacg ccgcaagcga tgtgatacac gtaaagaact ggttgttaga
     1561 cgaggtaagg ctggggcgga ggtacgggtg gcgcgctggc aaattcaagt gccatgcggc
     1621 acagcgcggg atggtcaacg ctgcagtcgg gaaaaatggg aacggtgatg gccacgggga
     1681 tggcgctcag atgggccagg aaaaaactct ggcagcactc gcaattaccg ggatcgcaat
     1741 cgggggaatc cgcaacggag acgcctgagt ccgaatggga aagcggcgcg accgcgtgat
     1801 cgtgatctgc gccctggcct gagccggcgt gcgcatgttc tggctgtgca tggttcgcgc
     1861 ggtcggggca gcaggcgaac tcgggaaagg cgctgcacgt gccagccgtg gtgaggaaca
     1921 gcagcagtgc gctgagcatg atggcgaccg atcgcctcgc acgccggaat ctacccaagg
     1981 tctgcaaaac gccacacgcc gtgtagtgtg cagatgattt cccaggggtt ggctgctgtc
     2041 ctgtgcactg ttccgagtgg acggccggcc tgccgtgtct gctcgaactg cggtgtgccg
     2101 cgcaagcgac cgtgaaagtc agggtcatgc gcgtgacttg tgacatcctt gtgacatggc
     2161 cacgtacatt gcatgccatt attctgtcag tcaatatctc ctgattatat tatctttttc
     2221 ttatacgccc ggacgagacc tccgatccgt tgttcggcag ggcgcgccgg acgagtggta
     2281 gatttcttgt cggtaagccg agacagtcat ggtcgctgtt tcgtttttgg ttttatcccg
     2341 ccatgcgttt gggcgcagca tggcgctcca acacatgcgt taaaaactct ccagtgtgtg
     2401 aggcgggatc ctgcgccacc ttctcgggcg tgccggtggc gaggattttc ccgccgccat
     2461 ccccgccttc agggccgaga tcaatcagcc aatcagcgg
//
LOCUS       DBNGZE010000451         7905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1243268, whole genome shotgun sequence.
ACCESSION   DBNGZE010000451 DBNGZE010000000
VERSION     DBNGZE010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1243268"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC16FV_11745"
     CDS             <1..277
                     /locus_tag="AC16FV_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850758.1"
                     /translation="NKFKFHWYEADDLEGPRGGSGTGGSLWALGVDHSFSKTAMVYFN
                     YASADNDTNASFVNVASGNGGHGNGVPLSALGEKSPTGYSAGVIVKF"
     gene            476..1507
                     /gene="argC"
                     /locus_tag="AC16FV_11750"
     CDS             476..1507
                     /gene="argC"
                     /locus_tag="AC16FV_11750"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR2850759.1"
                     /translation="MIKIGIIGGTGYSGVELLRLLAGHPQADLRVITSRGEAGKKVAD
                     LFPNLRGHVKLAFTEPDTKALSGCDVVFSATPNGVAMTHARELLKAGVKFIDLAADFR
                     LKDPAVWEKWYGVPHACPELLAEAVYGLPEVNRDKIKKARLIANPGCYPTAVQLGFLP
                     LLESGLVDTQHLIADAKSGVSGAGRKAEVHTLFSEAADSMKAYGVAGHRHWPEIRQGL
                     DAVLGKPVGLTFVPHLTPMIRGIHATLYARLTKSGADLQAIYEKRYANEPFVDVMPSG
                     SHPETRSVRVSNVCRIAVHQPQGGDTVVVLSVIDNLVKGAAGQAVQNMNILFGLDERT
                     GLHQAGVLP"
     gene            1570..2286
                     /locus_tag="AC16FV_11755"
     CDS             1570..2286
                     /locus_tag="AC16FV_11755"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR2850760.1"
                     /translation="MIDLNKPGELVIKQRYSPRMKLLLAVIAAVVLLAAAGFIYNYGL
                     NRAGFERQSAEQTQQTLQDDMRKLHDENQGLQESLARAQRTIQMDQAAYQDLDKSLKA
                     SAQEIVKLREELNFYRNIISPADKKSGLRIQNLYIEPAGGTNQYRYKLVLIQALKHES
                     VIQGRAILEISGMQVGEDALLRFPAANERPIQVNFKYFQDIEGKLELPKNFQPKRIKV
                     YIMTSGGTSMAEATYAWPLG"
     gene            2363..2833
                     /locus_tag="AC16FV_11760"
     CDS             2363..2833
                     /locus_tag="AC16FV_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR2850761.1"
                     /translation="MEGNAMLNALGKKPTDKPCNTIDTLVGVKTDLKGDIVFSGGLRI
                     DGKVRGNIIAKGDGNSTLVLSENASVTGNVTVPHIITNGSIKGNVRASERIELQSKAE
                     IFGDVYYKVIEMALGAVVNGNLVRETVEVGRDSTNKGTVTRLKSAGENGVDAED"
     gene            2907..3260
                     /gene="erpA"
                     /locus_tag="AC16FV_11765"
     CDS             2907..3260
                     /gene="erpA"
                     /locus_tag="AC16FV_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR2850762.1"
                     /translation="MSAAMPEMPSPLMFTDSAANKVKELIEEEKNPALMLRVFVSGGG
                     CSGFQYGFTFEENANEDDTRVEKNGVTLLIDPLSFQYLAGAEIDFQEGVQGAQFVIKN
                     PNAKTTCGCGSSFNT"
     gene            complement(3343..4452)
                     /locus_tag="AC16FV_11770"
     CDS             complement(3343..4452)
                     /locus_tag="AC16FV_11770"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR2850763.1"
                     /translation="MHYIGLMSGTSVDGIDAALVTLSAGSRPVLVSTHAHPIPATMRG
                     EIQTLMRDGPNEIERLGELDMALGELFAEAANAVIKKAGLAKKDIRAIGSHGQTLRHR
                     PRAAHPFTLQIGNPSVIADRTGITTVADFRARDMAAGGQGAPMVPAFHRQMFHSPERN
                     RAIINIGGIANVTYLPADVSQPVVGFDTGPGNTLLDQWINQHHAREHDEAGQWAASGR
                     VSKELLGQFLTDPYFMAAPPKSTGREHFNMEWLQAHLKKLPAPSSAADVQATLIQLTT
                     QTIAQAIRGFLPKTQEAYVCGGGAHNRELISALAGNMTGVAVATTDTLGLAPDWVEAA
                     AFAWLAHQTLERQPGNLPSVTGAKRAVILGGIYPA"
     gene            complement(4482..5759)
                     /locus_tag="AC16FV_11775"
     CDS             complement(4482..5759)
                     /locus_tag="AC16FV_11775"
                     /inference="COORDINATES: protein motif:HMM:NF039309.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HIR2850764.1"
                     /translation="MPRESELLPVNTLTPSMPPQTVQLGEVPVPDTATVTADSPAEAA
                     TPGKISERNQWIEVTIRKGDTLSDILKREGHDNSDIHRLVQSNPDARVFRQLQPGQKL
                     RLRVNAEGPLQELVYETDPGEAFHLVRHEDRLELTKELRTFETRVAHVSGTIESSLFE
                     DGQDAGLSDNLIMKLVEIFGWDIDFALDLRRGDSFSVIYEEKFWQGQKVTDGAILAAE
                     FVNQGRTFRAIAFRDTNGYVFYYAPDGTSVRRAFLRTPVEFSHITSRFTTSRYHPILK
                     TRRAHKGVDYAAPTGTPVRATASGRILSIGTQGGYGKRIVIRHNATYSTLYGHLSRFR
                     ADLHVGSFVDQGQTIGYVGATGLASGPHLHYEFQVNGEHRNPLTFKFAGGGKIASQNR
                     DEFARLASDWSARLDIISHGTRIAANRQAGNPL"
     gene            6043..7245
                     /gene="tyrS"
                     /locus_tag="AC16FV_11780"
     CDS             6043..7245
                     /gene="tyrS"
                     /locus_tag="AC16FV_11780"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR2850765.1"
                     /translation="MTSATEALRLIKRGTEEILLESELVERLKSGRRLRVKAGFDPTA
                     PDLHLGHTVHINKLRQFQDLGHEVLFLIGDFTGMIGDPTGKNSTRPPLSRDEVIENAR
                     TYENQIYKILDPEKTLVMFNSSWMGEMRAADLVQLAATHTVARMLERDDFSKRYKSGQ
                     PIAIHEFLYPLIQGYDSVAMRADVELGGTDQKFNLLVGRELQKLNGQTPQVVITMPLL
                     EGTDGANKMSKSLGNYIGIDESPDEMFGKLMSISDTLMWRYFDLLSFEHADKIASMKR
                     SVDEGANPRDIKFELAKEIVSRFHGGPKAGHDAMQNFIARFQQREIPADISEIELAGN
                     GRGLALPRLLKEAGLTSSTSEAMRLIKQGGVRIDGEKAEDPALEIHPGQAHVFQVGKR
                     KFMRVRVR"
     gene            7599..>7905
                     /locus_tag="AC16FV_11785"
     rRNA            7599..>7905
                     /locus_tag="AC16FV_11785"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1704 a         2139 c         2418 g         1644 t
ORIGIN      
        1 caacaagttc aagttccatt ggtatgaagc tgacgatctc gaaggccctc gtggtggttc
       61 gggcacgggc ggcagcctgt gggcgcttgg tgttgatcat agcttcagca agaccgcaat
      121 ggtttacttt aactacgcca gtgccgataa cgacaccaat gcgtcgtttg tcaacgtcgc
      181 cagcggcaac ggcggccatg gcaatggtgt cccgctatct gccttgggcg agaagtcgcc
      241 tactgggtat tcagcgggtg ttatcgtcaa gttctaagcg gaattcatct gtaacggttg
      301 aacaaacata aagcagtatc gctgacgggg cctttgggcc ccgttttttt tccagcggat
      361 ttcagactca agttgcctat gccgcgaaaa cgaccgacct ttcgagctcc ggcaatatcc
      421 tgtattgtta atttgaattg cgctattttt cagccaaatc actgtaatcg ggcacatgat
      481 caaaatcggc atcatcggtg gtaccggcta tagcggcgtg gaattgctgc gcctgctggc
      541 ggggcatccg caggctgatt tgcgggtgat cacctcgcgc ggcgaggcag gcaaaaaagt
      601 cgcggacctg tttcccaacc tgcgtgggca cgtaaaactc gccttcacgg aacccgatac
      661 gaaagcgctc tccggctgcg acgtggtgtt ttcggccacg cccaatgggg tcgccatgac
      721 gcatgcacgc gagttgctca aggctggcgt gaagttcatt gatctcgccg ccgattttcg
      781 tctgaaagat cccgctgtct gggaaaaatg gtacggcgtg ccgcatgctt gccccgagct
      841 gctggccgag gcggtttacg ggctgccgga agtgaaccgg gacaagatta aaaaagcgcg
      901 actaatcgcc aaccccggat gctacccgac cgccgtgcaa ttgggatttc tcccattgct
      961 ggaaagcggg ctggtggata cgcagcatct gatcgccgat gccaaatcgg gtgtcagcgg
     1021 cgcgggccgc aaggccgaag tgcatacgct gttttcggaa gcggcggatt cgatgaaagc
     1081 ttatggcgtc gcgggccatc gccactggcc ggagatccgc cagggactcg atgcggtgtt
     1141 gggcaagccc gtcggcctga ccttcgtgcc gcatctcacg cccatgatcc gcggcatcca
     1201 cgcgacactc tacgcacggc tcaccaagtc gggagcggac ttgcaggcga tatacgagaa
     1261 gcgctacgcc aatgaaccgt tcgtggatgt catgccttcc ggcagtcatc ccgagacacg
     1321 ttccgtgcgt gtctccaatg tctgccgtat tgccgtgcat cagccgcagg gtggcgatac
     1381 ggtggtggtg ctgtccgtca tcgataatct ggtcaagggc gccgccggtc aggcggtgca
     1441 gaacatgaac atcctgttcg gtctggacga acgcaccggt ctgcatcagg cgggggttct
     1501 gccctgataa ttgtgctttc aaccggcggt tgatgcggct atattcggaa tattccgcag
     1561 gccaacctaa tgatcgatct caataaaccc ggcgaactgg tcatcaagca gcgctattca
     1621 ccgcgcatga agctcctgtt ggcggtgatc gcggcggtgg tgttgctggc tgccgccggt
     1681 tttatctaca actacggact caatcgcgcc ggcttcgagc gccagtcggc ggagcagacg
     1741 cagcagacct tgcaggacga tatgcgcaaa ctgcatgatg aaaatcaggg gctgcaggaa
     1801 tcgctggcgc gcgcgcagcg caccatccag atggaccagg cggcctatca ggatctcgac
     1861 aagtcgctca aggcctcggc gcaggagatc gtgaagctgc gggaagagtt gaatttctac
     1921 cgcaacatca tttcgccggc cgacaagaaa agcggtctgc gcatccagaa cctctacatt
     1981 gaaccggccg gcggcacgaa ccagtaccgt tacaaactgg tgttgattca ggccctgaag
     2041 catgagagcg tgattcaagg gcgggcgatt ctggaaatca gcggcatgca ggtgggagag
     2101 gatgccttgt tgcgattccc ggccgcgaac gagcgtccga ttcaggtgaa tttcaagtat
     2161 tttcaggata tcgagggcaa gttggaattg ccgaagaatt tccaacccaa gcgcatcaag
     2221 gtctatatca tgacttccgg cgggacttcc atggcggaag cgacctacgc ctggccgctc
     2281 gggtagaatt ccggtttccg aatttcatcg ccccgggcgg gagcgccagg cttgcggtga
     2341 cggggtcaac tacaatgcat acatggaggg taacgcaatg ctgaatgctc tgggaaagaa
     2401 gccgaccgac aaaccgtgta acaccatcga tacgctggtt ggcgtcaaaa ccgatttgaa
     2461 gggcgatatc gtgttttcgg gtggtctgcg cattgacggt aaggtccgcg gcaacatcat
     2521 tgccaagggt gatggcaaca gcaccttggt gctcagcgag aatgcctccg tgaccggtaa
     2581 tgtcacggtg ccgcatatca tcaccaatgg ctcgatcaag gggaatgtcc gcgccagcga
     2641 gcgtattgag cttcagtcga aagcggagat attcggcgat gtgtattaca aggtgatcga
     2701 aatggccctg ggggccgtgg tcaacggtaa tctggtgcgg gagacggttg aagtcggccg
     2761 tgacagcacc aataaaggca cggtgacccg tctgaaatca gctggagaaa acggcgtgga
     2821 tgccgaagac tagcaattgt tgacccgcgg ggtcaggaat aggataattg ttcatcgcaa
     2881 agttactgta atttgtggag gttcaaatga gtgctgccat gcctgagatg ccgtcaccct
     2941 tgatgtttac cgacagtgcg gcaaacaagg tgaaagaatt gatcgaagag gaaaagaatc
     3001 cggcgctgat gctgcgggtg tttgtctccg gcggcggctg ctcgggcttt caatacggtt
     3061 ttacgttcga agagaacgcc aacgaagacg acacgcgcgt ggagaaaaac ggcgtcaccc
     3121 tgctgatcga tccactcagc ttccagtatc tggcgggcgc ggaaatcgat tttcaggaag
     3181 gcgtccaggg cgctcagttc gtgatcaaga acccgaatgc caagaccacc tgcggctgcg
     3241 gttcctcgtt caatacctaa acaccgactt aatcaggaaa cagcaaaagg gcccattcgg
     3301 gcccttttgc atttctgcag gtaagaaatg ttcgggcgtg tatcaggcgg gataaatacc
     3361 cccgaggatg acagcgcgtt ttgcgccagt gacggaaggc agattgccgg gttgtctttc
     3421 cagggtctga tgcgccagcc aggcgaaggc cgccgcttct acccagtccg gcgcaaggcc
     3481 aagggtatcg gtagtagcga ctgcgacgcc ggtcatgttg ccagccagtg ctgagattaa
     3541 ctcgcggtta tgtgcgccgc cgccgcagac ataggcctcc tgcgtcttgg gcagaaaccc
     3601 gcggatcgcc tgcgcgatgg tctgtgtggt gagctgaata agtgttgcct gtacatcggc
     3661 tgcggaagac ggcgcgggaa gttttttcag atgagcttgc agccattcca tgttgaagtg
     3721 ttcgcgcccc gtgcttttgg gaggcgcggc catgaaataa ggatcagtca ggaactgtcc
     3781 caacagctct ttcgagaccc gtccggaggc ggcccattgt ccggcctcat catgctcgcg
     3841 tgcatgatgt tgattgatcc attggtcgag caaggtatta ccggggccgg tgtcgaagcc
     3901 gactactggc tgtgatacat cggctggcag gtaggttacg ttggcgatcc cgccgatgtt
     3961 gatgatggcg cggttccgtt cgggggagtg aaacatctgg cgatgaaatg ctggcaccat
     4021 cggtgcgccc tgaccgccgg cggccatgtc gcgcgcgcga aaatccgcca cggtggtgat
     4081 gccggtgcga tcggcgatca cggacgggtt gccgatctgg agcgtaaagg gatgggccgc
     4141 gcgcggccgg tgccgcaacg tctgaccatg gctgccaatg gcgcggatat ctttttttgc
     4201 gagccctgct ttcttgatca cggcgttcgc ggcctcggcg aacagctccc ccagcgccat
     4261 gtcgagttct cccagccgtt cgatttcgtt gggaccatcg cgcatcaggg tctgaatctc
     4321 gccacgcatc gtggcaggga tggggtgggc gtgggtggag accagaacgg gccggcttcc
     4381 cgcggagagg gtaaccagcg cggcatcaat gccgtccacg ctggttcccg acatcagccc
     4441 gatgtaatgc atgagttcag tttcgggcag cccgtggaaa gttacaaggg gttcccggct
     4501 tggcgattgg cggcgatgcg cgtaccgtga ctgatgatat ccaggcgcgc agaccagtca
     4561 ctggcaagcc gcgcgaactc atcgcgattc tgtgaggcga tctttccgcc gcccgcaaac
     4621 ttgaaggtca gaggattgcg gtgttccccg ttgacctgaa attcgtaatg caggtgggga
     4681 ccgctcgcga ggccggtggc gccgacatag ccgatggtct ggccctgatc cacgaaactg
     4741 ccgacatgga gatcggcgcg gaagcgcgag agatgtccgt agagcgtgct gtacgtggcg
     4801 ttatggcgta tgacgatgcg cttgccgtaa ccgccctgcg tgccgatgga caggatgcgt
     4861 ccgctggccg tggcgcgcac cggtgtgccg gtgggggcgg catagtccac gcccttgtgc
     4921 gcccgccggg tcttgaggat agggtgataa cggctggtgg tgaaacgcga ggtgatatgg
     4981 ctgaactcca ccggagtgcg caggaacgcc cgacgcacgc tcgtgccgtc gggcgcataa
     5041 taaaaaacgt agccattggt gtcgcggaag gcgatagcgc ggaacgtccg cccctgattg
     5101 acgaactcgg cagccagaat agccccgtcg gtgacctttt gcccctgcca gaatttttct
     5161 tcgtatatga ccgagaaact gtcgccacgg cgcaggtcga gggcaaagtc gatatcccag
     5221 ccgaagattt ccaccagctt catgatcagg ttgtccgaga gtccggcgtc ctgaccgtct
     5281 tcgaacagag aggactcgat ggtgccggag acatgggcca cgcgcgtttc gaacgtgcgc
     5341 aactccttgg tgagctcaag gcgatcctcg tggcggacca gatgaaacgc ctcgccggga
     5401 tcggtttcgt agaccagttc ctgcaatggc ccttcagcgt tgacacgcag gcgcagtttc
     5461 tgtcccggct gcaactggcg gaacacgcgg gcatcggggt tcgactgaac caggcgatgg
     5521 atatcgctgt tgtcatgacc ttcacgcttc aggatatccg agagcgtgtc gcctttccgg
     5581 atggtgacct cgatccactg attgcgttca gatatttttc cgggagtcgc cgcctcggct
     5641 ggcgagtctg ccgttacagt ggcggtatcg ggaacaggca cctcgccaag ctgtactgtt
     5701 tgtggcggca tggaaggggt cagcgtgttc accggcagca actcgctttc gcgcggcatg
     5761 atggccgcga tagtgaccga tgtcaggaaa accgcgagcc ccagaagcca tacgccacgc
     5821 ccgggaaggc ggtgggcatg ggctcggccg gcacggcgaa gcgctgcggc aaattttttt
     5881 aacgatcgtt tgggcaaagg aaacgctgcc ttaaaagtaa aaactgtacg ctaaagttta
     5941 gaccagcagc accgcggcgg ggttgattgc cagcatgcca tgttaaagcg gctatggtac
     6001 gctttgcagc gcccggaatc acctgccgtt tcaggaacat ccatgacatc agccaccgaa
     6061 gcgctgcgtc tcatcaagcg cggcaccgaa gaaatcctgc tcgagtcaga gcttgttgaa
     6121 cgcctcaagt ccgggcggcg gctgcgcgtg aaggcgggct tcgatccgac cgcccccgat
     6181 ctgcatttgg gacacaccgt acacatcaac aagctgcgcc agttccagga cctaggccat
     6241 gaggtgctgt ttctcatagg cgatttcacc ggcatgatcg gcgatcccac cggcaagaac
     6301 agcacacgcc cgccgctgtc tcgcgacgag gtgatcgaaa acgcgcgcac ctatgaaaac
     6361 cagatttaca agattctcga tccggaaaag acgctggtga tgttcaattc gagctggatg
     6421 ggcgagatgc gtgccgccga tctggtccag ctggcggcca cgcacacggt ggcgcgcatg
     6481 ctcgagcgcg acgacttcag caagcgctac aaatccggtc agcccatcgc gattcacgaa
     6541 tttctttatc ccttgattca gggttacgac tcggtcgcca tgcgcgccga tgtcgaactc
     6601 ggcggtaccg atcagaaatt caatttgctg gtcggacgcg aattgcagaa gctcaacggc
     6661 cagacgcctc aggtcgtgat caccatgcca ttgctcgaag gcaccgacgg cgcgaacaag
     6721 atgtcgaagt cgctcggcaa ttacatcggc atcgatgaat cgccggacga aatgttcggc
     6781 aagctcatgt cgatttcgga cacattaatg tggcgctatt tcgatctgct ctcgttcgag
     6841 cacgctgaca agattgcatc gatgaagcgc agtgtcgatg aaggtgccaa tccgcgtgat
     6901 atcaaatttg aactggctaa ggaaatcgtt agccgttttc atggcggacc gaaagcaggc
     6961 cacgatgcaa tgcaaaactt tatcgcgcgt tttcagcagc gggaaatacc ggcggatatt
     7021 tccgaaatcg aactggccgg caatggcagg ggtctcgcgc ttccccgtct cctcaaggaa
     7081 gcgggcctca cgtccagcac ctcggaggcc atgcgcctca ttaagcaggg cggtgtgcgg
     7141 atcgacggcg agaaggcgga ggatccggcg cttgaaatcc acccgggaca ggctcatgtt
     7201 tttcaggtcg gcaagcgcaa attcatgcga gtacgcgtcc gttgataccc gattaaacgg
     7261 gtttatttca agttggtggg caaaacgggc aaaaaccgat ctttttgatt accggaaaca
     7321 gcagttttcc agccattttt ccgtgtctga ttgcatctgc caacaggacg caaaataact
     7381 aaaatcaagc gttgacgcgg ggagcagcgc gcgtataatg cgcctccctt gggccggtac
     7441 cctgtatcgg ccttcgttct ttaaaaattc aagatcaagt aatttgtgtg ggcgctcttg
     7501 tcgagcctgg tcatccaatg aaaggtgact gatgcccgcc ttaaccggcg agttcagtca
     7561 catgtagttt tcgttccgtt aatcggaaca acaaagttta tttggagagt ttgatcctgg
     7621 ctcagattga acgctggcgg catgcctaac acatgcaagt cgcacgcgaa agggggcaac
     7681 cctgagtaga gtggcggacg ggtgagtaac gcgtaggaat ctacctggta gtgggggaca
     7741 acccggtgaa aaccgggcta ataccgcata cgatccacgg atgaaagcag gggaccgcaa
     7801 ggccttgcgc tatcagatga gcctgcgtcg gattagctag ttggtgaggt aaaggctcac
     7861 caaggcgacg atccgtagct ggtttgagag aacgaccagc cacac
//
LOCUS       DBNGZE010000452         2073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_966380, whole genome shotgun sequence.
ACCESSION   DBNGZE010000452 DBNGZE010000000
VERSION     DBNGZE010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_966380"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="AC16FV_11790"
     CDS             <1..465
                     /locus_tag="AC16FV_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2850766.1"
                     /translation="ARTSPEAIYKTMRFYRDIGKHPIHIKKEVRGHVANRLQAALWRE
                     AIHLVTEGVVSVADADAAIAYGPGLRWALMGPSLTFHMAGGEGGMKHFMEHIGPAIQR
                     WMDDLGAPKLTPKTQQAIIDGVTEEAAGRSRADLQRWRDRKLIEILKVSGEP"
     gene            499..951
                     /locus_tag="AC16FV_11795"
     CDS             499..951
                     /locus_tag="AC16FV_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR2850767.1"
                     /translation="MHRYLTLALFVALVLGGGTLIGLMTLPGEWYAGLAKPSFNPPNW
                     IFAPVWTLLYIMVAVAGWRTWQHSPRSAAMAVWFIQLALNFVWSPTFFGAHRIGAAQV
                     IVVALLTTIIAFIVMRWPGDRAAALLFMPYAAWVAFATLLNGALWYLN"
     gene            complement(976..1203)
                     /locus_tag="AC16FV_11800"
     CDS             complement(976..1203)
                     /locus_tag="AC16FV_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HIR2850768.1"
                     /translation="MARRPTSDSRSEAEKAFKAATTKPVEQPAAKAPSLPGAKEMVSL
                     RIDRDVLDFFQEDGPGWQDRINAALRKAVGK"
     gene            1367..2073
                     /locus_tag="AC16FV_11805"
                     /pseudo
     CDS             1367..2073
                     /locus_tag="AC16FV_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
BASE COUNT          377 a          679 c          632 g          385 t
ORIGIN      
        1 gccaggactt cgccggaagc catttacaag acgatgaggt tctatcgcga catcggcaag
       61 cacccgattc acatcaagaa ggaagtgcgc ggccacgttg ccaaccgcct gcaggcagcg
      121 ctgtggcgtg aagccatcca tctcgtcacc gaaggagtcg tttccgtcgc ggatgcggac
      181 gcggcgatcg cgtatggtcc cggcctgcgg tgggcgttaa tggggccgtc cttgacattt
      241 cacatggccg gcggcgaagg cggcatgaaa catttcatgg agcacatcgg cccggcaatt
      301 cagcgctgga tggacgacct cggcgcgccc aagctgacgc caaaaacgca acaggcgatc
      361 atcgacggcg tcaccgagga agcggccggc cgcagcaggg ccgacctcca gcgctggcgc
      421 gacaggaagc taatcgagat tctgaaggtg tcgggggagc cctgatgtcc gacgatgcga
      481 atttggtgag ttgatagaat gcaccgctac ctcaccctcg ccctgttcgt cgctctcgtg
      541 ctcggtggcg ggacactgat cggccttatg acactgccgg gcgaatggta cgccgggctc
      601 gccaagccgt cgttcaatcc gccgaactgg atcttcgcgc cggtgtggac gctgctatac
      661 atcatggtcg cggtcgccgg ctggcgcacc tggcagcaca gcccacgcag cgccgcgatg
      721 gcggtatggt tcatccagct cgcgctcaat ttcgtctggt cgccaacgtt ctttggcgcg
      781 catcgcatcg gcgccgcgca ggtgatcgtg gtggcgctgc tgacaacaat catcgctttc
      841 atcgtgatgc gctggccggg tgatcgtgcc gccgcgttgc tgtttatgcc ttatgcagcg
      901 tgggtggcgt tcgccacgct gctcaacggc gcgctctggt atctcaattg agcttaccgc
      961 gggcggaggt cggcgctact tccccaccgc cttgcgcagc gcggcgttga tgcggtcctg
     1021 ccagccgggg ccgtcttcct gaaagaagtc gagtacgtcg cggtcaatgc gcaatgacac
     1081 catttccttg gcgccgggca gcgacggtgc ctttgccgcc ggttgctcga ccggcttagt
     1141 ggtcgccgcc ttgaaagctt tttcggcctc agagcgggag tcggatgttg ggcgtcgagc
     1201 cataagttta attgtatcac acgccccaca aaaaacccgt caggcacaaa cttgctccgc
     1261 tcgtccccgc gaaagcggag atcatggcgc gggcgcaagg gacacacttc gccccagcca
     1321 aaacgaccgt tatgcttgcc tcgctccatc caaggggaca ccacacatgc cttccctcct
     1381 gcaaaaccac accgcggtcg tgaccggctc cggctccggg atcggccgcg ccatcgcgct
     1441 gggctacgcg cgcgaggggg cgcaggtcgt cgtgctcgac atcaatggcg cggcagcagc
     1501 ctcgacggcc gcggacatct gcaaagccgg cggcgaagta tttcacgctc gacgtcaccg
     1561 atcgagccgc ctgccgcgct gccgccgcag acgtcgctgg aacagtcggc caggtctcga
     1621 tcctcgtcaa caatgccggc atcaaccggc gcaacgcctt tacttccgat ccagacgccg
     1681 tcatcaagga ctggcaggac atcatggcga tcaatctcaa cggtttgttc aacgtaacac
     1741 acgcctttct tggcgccctg cgcgccagca aggggcgcat catcaacatc ggctcgatcc
     1801 agtcgttcgt gcatgtacgg acgcccaatt cggtggccta tacgacgtcg aagcacggcg
     1861 tgctcggctt cacccgcgca ctcgccgctg agctcggcta ggagggcgtg cgcgtcaacg
     1921 ccatcggccc aggtctgatc gaaacaccgc tcaacgcgca agtgcgtgct aacaatccgg
     1981 agctggtgaa gatctttctc acccacacgc cgctcggccg cgccggtaag ccggaggaca
     2041 tcgtgggccc tgcgattttc ctcgcctccg atc
//
LOCUS       DBNGZE010000453         4459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1868663, whole genome shotgun sequence.
ACCESSION   DBNGZE010000453 DBNGZE010000000
VERSION     DBNGZE010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1868663"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC16FV_11810"
     CDS             complement(<1..621)
                     /locus_tag="AC16FV_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR2850769.1"
                     /translation="MAKQDYYEVLGVARNASEADIKKAYRRLAMKHHPDRNPDKSAEA
                     KFKEAKEAYEILSDAQKRAAYDQFGHAGVDPSAAAGAAGGFYSAGGANFADIFGDVFG
                     DIFGGGRTRGAQVFRGANLRYSLELSLEDAVRGTEVRIRVPAMETCEACQGSGAKSGT
                     SSTICRTCGGHGQVRMQQGFFSVQQTCPKCHGAGKIITDPCPTCHGA"
     gene            complement(728..2677)
                     /gene="dnaK"
                     /locus_tag="AC16FV_11815"
     CDS             complement(728..2677)
                     /gene="dnaK"
                     /locus_tag="AC16FV_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR2850770.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGDKARVIENSEGDRTTPSVVAYTE
                     DGEVLVGQVAKRQAITNPANTLYAVKRLIGRKYEEEMVQRDVKMVPYKIVKAKNSDAW
                     VEARGKQMAPPEVSARVLQKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKKPGDSKIAVYDLGGGTFDISIIEIAEVDKEHQFEV
                     LSTNGDTFLGGEDFDKRIIDYLADEFKKDNGVDLHKDPLALQRLKDAAEKAKIELSSS
                     QQTEVNLPYITADASGPKHLNIKITRAKLESLVEDLIQKTIEPCKIALKDAGLKAADI
                     NDVILVGGQTRMPKVQQLVKDFFGKEPRKDVNPDEAVAIGAAIQGGVLGGQVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKASQVFSTADDNQTAVTVHVLQGEREMAGG
                     NKSLGRFDLGDIPPAPRGVPQIEVAFDIDANGILHVSAKDKATGKENKIIIKAGSGLS
                     EEEIQKMVKDAEAHAEEDHKARELAEARNHADSLVHSVRKSMKEMGDKLASEEKDKIE
                     VAIKNLEEAARGDDKDDIKSKTDALMEASHKLAEQMYAQANATAGAGAAEAAAGAGKN
                     EDDGKAKENVVDAEFEEVKESKDKK"
     gene            complement(2739..3338)
                     /gene="grpE"
                     /locus_tag="AC16FV_11820"
     CDS             complement(2739..3338)
                     /gene="grpE"
                     /locus_tag="AC16FV_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018876095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR2850771.1"
                     /translation="MSQYKEGPAEEESIAPAPQTDAPHDLEPATETQQLQAELEKLRA
                     EAAENLDRFLRARAEAENMRKRGELELAATRKFAIERFASEIVAVRDSLEFARMVELP
                     KDTNPAVQKMHEGLDLTLKLLDGVFQKFGLVLLDPRGQKFDPERHQAISMVDSVEVPP
                     GHVVSVVQKGFLLNDRLLRPAMVVVARAAAEAHKPAGQA"
     gene            complement(3380..4444)
                     /gene="hrcA"
                     /locus_tag="AC16FV_11825"
     CDS             complement(3380..4444)
                     /gene="hrcA"
                     /locus_tag="AC16FV_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR2850772.1"
                     /translation="MSLDARAEILLKTLIERYIVEGQPVGSRTLARQAGLDLSSATVR
                     NVMSDLEEMGLVRSPHTSAGRVPTERGYRVFVDTLLKVKPMNVNELHKLEQELHADYD
                     PQHLIENASHMLSEVCKLAGIVMMPRREEQLAFRHIDFVNLSGRRVLVILVTQDGQVH
                     NRIIHSDREYSPAELVQSANYFNETYSGISMGDVKRALLQEIQQASDDMQRIMQVAVS
                     MAREALSSEEQPDELVVSGESNLMDFPELGDVRKLRKLFDAFNAKRDLLHLLDQSMRG
                     PGVKIFIGQESGYDALAECSVVTAPYRADGRIVGTLGVIGPTRMAYEQVISIVDITAR
                     LLGGALSSSERPGRLESSGR"
BASE COUNT          741 a         1403 c         1308 g         1007 t
ORIGIN      
        1 agcaccatga caggtcggac agggatcggt aatgatcttt ccggcgccgt gacatttcgg
       61 acaggtctgc tggacggaaa agaaaccctg ctgcatgcgc acctggccgt gaccgccgca
      121 ggtgcgacaa atcgtggacg acgtgccgga tttggcgccg ctgccctgac aagcctcgca
      181 ggtttccatc gcgggaacgc gaatgcgcac ttccgtgccg cgcacggcgt cttcgagcga
      241 gagttcgagg ctgtagcgga gattcgcgcc gcgaaagacc tgcgccccgc gcgtacgacc
      301 ccctccgaag atgtcgccaa acacgtcgcc gaagatatcg gcgaagttcg cgcctccggc
      361 ggaataaaaa ccacctgcgg cgccggcggc ggccgaggga tccacgccgg cgtgtccgaa
      421 ctggtcgtag gccgcgcgtt tctgcgcgtc ggaaaggatt tcgtaggctt ccttggcctc
      481 cttgaatttg gcctcggcgg atttatccgg gttacggtcc ggatggtgtt tcatcgccaa
      541 acgtcggtag gccttcttga tgtcggcctc cgaagcgttg cgtgccacac cgagcacctc
      601 gtagtaatcc tgcttcgcca tgccagctac ctgttctgtt tatatttgct cacatcaaac
      661 agcaaaggca cagtggtcaa tccttgtcag accgcccacc atgcccttgt tgccgggaag
      721 atcgaagtta cttcttgtcc ttgctttctt tcacttcctc gaattcggca tcgacaacat
      781 tttccttcgc tttgccgtcg tcttcattct tgccggcgcc agccgctgct tccgccgcgc
      841 cggcacccgc cgtggcattg gcctgggcat acatctgctc cgccagcttg tgcgaagcct
      901 ccatcagggc atcggtcttg ctcttgatgt catccttgtc gtcgccgcgc gcggcctctt
      961 ccagattctt gatggccacc tcgatcttgt ccttttcctc ggaggccagc ttgtcgccca
     1021 tttccttcat ggatttgcgc acgctgtgga ccaagctgtc ggcatggttg cgcgcctcgg
     1081 cgagctcgcg cgccttgtgg tcttcctcgg catgcgcctc ggcatccttg accattttct
     1141 ggatctcttc ctcggacagt cccgagccgg ccttgataat gatcttattc tccttgccgg
     1201 tggctttgtc tttcgccgac acatgcaaaa taccgttggc gtcgatgtcg aatgcgacct
     1261 cgatctgtgg cacgccgcgc ggcgccggcg gaatgtcgcc gagatcgaaa cggccgagcg
     1321 atttgttgcc ccctgccatc tcgcgctcgc cctgcagcac gtgtaccgtt accgccgtct
     1381 ggttgtcatc ggcggtggaa aacacctgcg aggccttggt cggaatcgtg gtgttcttct
     1441 gaatgagttt ggtcatgacg ccgcccaacg tttcgatacc gagcgaaagc ggggtcacgt
     1501 cgagcagcag cacgtccttc acctgaccgc ccagcacgcc gccctggatc gcggcgccaa
     1561 tggcgaccgc ctcgtccggg ttcacgtcct tgcgcggctc tttgccgaag aaatccttca
     1621 cgagctgctg caccttgggc atgcgggtct ggccgccgac caggatgacg tcgttgatat
     1681 ccgcggcctt gaggccagcg tccttgagcg cgatcttgca cggctcgatg gttttctgaa
     1741 tgaggtcctc gaccagcgac tcgagcttgg cgcgcgtgat cttgatgttg agatgtttcg
     1801 ggccgctcgc gtccgcggtg atatacggca agttcacctc ggtctgctgg ctggaagaca
     1861 actcgatctt ggccttctcc gcggcatcct tcaggcgttg cagcgcgagt ggatccttgt
     1921 gcagatccac gccgttgtcc tttttgaatt cgtcggccag gtaatcaatg atgcgtttgt
     1981 cgaagtcctc accgccgaga aaggtatcac cgttggtgga cagcacctcg aactggtgct
     2041 ccttgtccac ttcggcgatt tcgatgatgg aaatgtcgaa cgtgccgccg ccgagatcgt
     2101 acacggcgat cttggaatcg cccggtttct tgtccatgcc gaaggcgagc gccgccgccg
     2161 tcggctcgtt gatgatgcgc ttgacctcga gaccggcgat gcgtccggcg tccttggtcg
     2221 cctggcgctg ggaatcgttg aaataggccg gaacggtaat gaccgcttcc ttcacttcct
     2281 cgccgagata atcctcggcg gtctttttca ttttctgcaa cacgcgcgcg gaaacttccg
     2341 gcggcgccat ctgcttgcca cgcgcttcga cccacgcatc gctgttcttg gccttgacga
     2401 tcttgtacgg caccatcttc acgtcgcgct gcaccatctc ctcttcgtac ttgcggccga
     2461 tcaggcgctt gacggcatag agcgtattcg cggggttggt gatggcctgg cgcttggcga
     2521 cctgacccac gagcacttca ccatcctcgg tataagccac taccgagggc gtcgtgcgat
     2581 cgccttcgct gttttcgatc acgcgagcct tgtctccttc catgatggcc acgcaggagt
     2641 tggtggtgcc caggtctata ccgataattt ttgccatggt tatcgcctca agagatgctg
     2701 taatttaggg ttcaaaatgg ggctgggtag gccggttttc aagcttggcc ggccggctta
     2761 tgcgcttcgg ccgccgcgcg cgccaccacc accatggccg gccgcagcag gcggtcattc
     2821 agcaaaaatc ccttctggac cacgctcacc acgtggcccg gcgggacctc gacggaatcg
     2881 accatgctga tggcctgatg gcgttcgggg tcgaattttt gcccccgcgg gtcgagcaga
     2941 accaggccga atttctggaa aacgccatcc agcagtttca gggtcaggtc cagcccctcg
     3001 tgcattttct gcaccgccgg gttggtgtcc ttgggcagct ccaccatccg cgcaaactcc
     3061 aggctgtccc ggaccgcgac gatttcggag gcaaaacgtt cgatggcgaa tttgcgcgtg
     3121 gccgcaagct ccagctctcc gcgcttgcgc atgttctccg cctcggccct ggcacgcaga
     3181 aaccggtcca ggttttccgc ggcttcggcg cggagctttt ccagctccgc ctgtaattgc
     3241 tgggtttcgg tggccggctc cagatcatgc ggcgcatccg tttgcggcgc cggtgcgatg
     3301 ctctcctcct ccgccgggcc ctccttatac tgactcatta caatacctcc acgctgaatg
     3361 gactcataaa gaattggggt taacgaccgg aggattcaag acggccgggg cgctcgctgc
     3421 tgctcaaggc gccgcccagg agccgggcgg taatatcgac gatggatatc acctgttcat
     3481 aagccatgcg cgtggggccg atcactccca aggtgcccac gatacggccg tcggcgcggt
     3541 agggcgccgt gaccacgctg cattcggcca gcgcgtcgta gcccgattcc tggccgatga
     3601 atattttcac gccggggccg cgcatgctct ggtccagcag atgcaacagg tcgcgcttgg
     3661 cattgaaggc gtcgaataat ttgcgcagct tgcgcacgtc ccccagctcg gggaaatcca
     3721 tgagattgga ttcgccgctg accaccagct cgtcgggctg ttcctcgctg ctcagggctt
     3781 cgcgcgccat gctcaccgcg acctgcatga tgcgctgcat gtcgtcgctg gcctgctgta
     3841 tttcctgcag cagggcgcgc ttcacgtcgc ccatggaaat accggaatag gtctcgttga
     3901 aataattggc ggattgcacc agctcggcgg gggaatattc gcggtccgag tggatgatgc
     3961 ggttatgcac ttggccgtcc tgcgtcacca gaatcaccag cacgcgtcgc cccgaaaggt
     4021 tcacgaaatc gatgtggcgg aaggccagct gttcctcgcg ccgcggcatc atgacgatgc
     4081 ccgcgagctt gcagacttcg gacagcatat gcgaggcatt ctcgatgagg tgctgcgggt
     4141 cgtaatccgc gtgcagctcc tgctcgagct tgtgcaactc gttgacgttc atgggtttga
     4201 ccttcagcag ggtatccacg aacacgcgat agccgcgctc cgtgggcacg cgcccggccg
     4261 aggtgtgcgg ggagcgcacc agccccattt cctcgagatc ggacatgaca ttgcgcacgg
     4321 tggccgagga caaatccagc ccggcttggc gcgccagggt gcgcgaaccc acgggctgac
     4381 cctcgacgat gtatcgctcg atcagggttt tcagcagtat ttccgcgcgt gcgtccaggg
     4441 acatgtcgaa ttctctata
//
LOCUS       DBNGZE010000454         2160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_132772, whole genome shotgun sequence.
ACCESSION   DBNGZE010000454 DBNGZE010000000
VERSION     DBNGZE010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_132772"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16FV_11830"
     CDS             <1..299
                     /locus_tag="AC16FV_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850773.1"
                     /translation="DAGEYWQYPVFGVALFDHKTRAIVQMVSQALQGRFEGLRCYAIV
                     YGGVQWWIGIASHRSPELESICLQTDGRLPLAAIPWTELGLIQSASKLLQGVRT"
     gene            complement(380..1018)
                     /locus_tag="AC16FV_11835"
     CDS             complement(380..1018)
                     /locus_tag="AC16FV_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR2850774.1"
                     /translation="MKLISVNVGRPRPVPHEGRVVSTGIFKEPVAGTVFLRRLNLDGD
                     GQADLRVHGGEYKAVYAYPFEHYVFWAGELGRNDFSHGQFGENFTITGWLEDAVCIGD
                     EFLISEARVQVTQPRSPCFKLGIRMGDDQFPARFAAANRTGFYMRVLQEGRVSAGDAI
                     ERVAHDPDSLSVRDVFRLRHGSGTRAEYERAARLAALSPSWRAAFEKRLVDG"
     gene            1106..1510
                     /locus_tag="AC16FV_11840"
     CDS             1106..1510
                     /locus_tag="AC16FV_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850775.1"
                     /translation="MLHWFRTELKTLARGTLALAGALWLIAAGAPCVMAQTPEPDPAP
                     NHCPERAKHNGAGHAAMPDCGPVTAISCQLPDTDTPLAAGFGDFAMTPVLLVTLPVPV
                     LLSDNGQPSRPDFFAPDIHAPPLHIQHLTLIL"
     gene            1569..>2160
                     /locus_tag="AC16FV_11845"
     CDS             1569..>2160
                     /locus_tag="AC16FV_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2850776.1"
                     /translation="MSIRTVLSRLAGILIIQLAAVSSVLAGPELTLAEAERLAIEHAP
                     WLQHHRTNADAAAERAVYESRLPDPQLTLGFLNVPTDSYRLDQEDMTMTMVGIRQSFP
                     PGSTLKLKGQRAEKELSREQVRVDMERRNLLRQVRQTWIDLYFQQQSLNTLEELRRLA
                     RKQLEASEARYRAAQEMQQTVLKARQALARVNEREPM"
BASE COUNT          418 a          707 c          622 g          413 t
ORIGIN      
        1 gagatgctgg agagtactgg caatacccag tgtttggggt ggccctgttt gatcacaaaa
       61 cccgtgccat cgtccagatg gtcagccaag ccctgcaagg ccggttcgaa gggctgcgct
      121 gctacgccat tgtctacggt ggtgttcaat ggtggatagg cattgcttca caccgcagtc
      181 cggaactaga aagcatatgc ctgcaaaccg atggtcggct tccactggcc gccataccct
      241 ggactgagct aggtttgatt cagtcagcct caaagctact gcaaggcgta cgaacctaat
      301 cctccgctca accggagagc ccaactgtgt cccgcagttg gctctccgct aagctcgaac
      361 attacatgta acattccacc tatccgtcca ccagccgttt ctcaaaagcc gcccgccagc
      421 tcggtgacag tgccgccagg cgcgcggcgc gttcgtattc cgcgcgcgtg ccgctgccat
      481 gccgcagccg gaacacgtcg cgcacgctga gagaatcggg atcgtgcgca acccgctcga
      541 tggcatcgcc cgcgctgact ctgccctcct gtagtacccg catataaaaa ccggtgcgat
      601 tggccgcggc gaagcgcgcg gggaattggt cgtcgcccat gcggatgccg agcttgaaac
      661 agggcgagcg cggctgggtg acctgcaccc gcgcctcgct gatcagaaac tcgtcaccga
      721 tgcagaccgc gtcttccagc cagccggtga tagtgaaatt ctccccgaac tgtccgtggg
      781 aaaaatcgtt acgccccagc tcgccggccc agaagacgta atgttcgaac gggtaggcat
      841 agaccgcctt gtattccccg ccgtgcacgc gcagatcggc ctggccgtcg ccatcgaggt
      901 tcaggcgccg caggaatacc gtcccggcca ccggttcctt gaatatgccg gtactcacca
      961 cccggccctc gtggggcacc ggacggggcc tgccaacatt gacggaaatc aatttcatcg
     1021 ccttgtctct gcgtagaatg ggatttatcc gggatgtcgc cggtcaatgt ataccgtaat
     1081 ccgttccggg actaaactgt cattcatgct gcactggttt cgcactgaac tgaaaaccct
     1141 cgcccggggg acgctcgcct tggcgggagc gctgtggctg atcgccgccg gcgctccctg
     1201 tgtcatggcg cagacgccgg agccggatcc cgctcccaac cattgcccgg aacgcgcgaa
     1261 gcataacggc gccggacatg ccgcgatgcc ggattgcggt cccgtgactg cgatcagctg
     1321 ccagttgccc gacacggaca cgccgctcgc agccggcttc ggcgatttcg ccatgacgcc
     1381 ggtgttgctg gtcaccctgc cggttccggt gctgctgtcg gataacggcc agccgtcgcg
     1441 gcccgatttc tttgcccccg atattcacgc gcctccgctt catattcaac acctcacgct
     1501 gatcttgtag tctctctcct gcgcgttcgc gcgccgtcgt ttatgttcac gactgcaagg
     1561 agagatttat gtccattcgt accgttttat cccgcctcgc gggaattctg atcatccagc
     1621 tggccgctgt atccagcgtc ctggccggac ccgaattgac cctggccgag gccgagcgcc
     1681 tggcgatcga gcacgcgcca tggttgcagc atcatcgcac caacgccgat gccgcggccg
     1741 aacgcgcggt gtatgaaagc cggctccccg atccacaact cacgttgggt tttctcaacg
     1801 taccgacgga ttcctaccgg ctcgaccagg aagacatgac catgaccatg gtcggcatcc
     1861 gccagtcgtt tccaccgggc agcaccctca agctcaaggg acaacgcgcc gagaaggaac
     1921 tctcgcgcga acaggtgcgc gtggacatgg agcgccgcaa tctgttgcgg caggtacgcc
     1981 agacctggat cgatctctat ttccagcaac agtcgctgaa cacgctggag gagctgcgcc
     2041 gcctggcacg caaacaattg gaagcatcgg aggcgcgcta tcgcgccgcg caggaaatgc
     2101 agcagacggt actgaaggcg cgacaggcac tggcgcgcgt aaatgaacgc gaaccgatgc
//
LOCUS       DBNGZE010000455         2699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1174656, whole genome shotgun sequence.
ACCESSION   DBNGZE010000455 DBNGZE010000000
VERSION     DBNGZE010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1174656"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1139)
                     /gene="dnaQ"
                     /locus_tag="AC16FV_11850"
     CDS             complement(393..1139)
                     /gene="dnaQ"
                     /locus_tag="AC16FV_11850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR2850777.1"
                     /translation="MRQIILDTETTGLEPAEGHRIIEIGCVELINRRASNNRYQQYIN
                     PEREIDAGAMEVHGITNEQLASKPKFADIAQSLLDFIQDAELIIHNAPFDVGFLNSEL
                     SRVANGGKPVRVEEICSVVDTLKLARSMHPGQKNDLDSLCRRYNVDNSHRTLHGALLD
                     AEILADVYLAMTGGQTGLFEDTHSHLRGATTGNIAAVPSDIRRLDPNRPKLPVIAATA
                     EELAAHQAWLEELDKKSGGKCLWKKLEPAS"
     gene            complement(1163..2011)
                     /locus_tag="AC16FV_11855"
     CDS             complement(1163..2011)
                     /locus_tag="AC16FV_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850778.1"
                     /translation="MIEKIPATFWGVVIGAFFSLGGVALTNRANDRRLHAQLAHDRDM
                     KSRERELSLRRDVYLAAAEAVSAGLITVSQFSNLDIPHDKLTEAYIDKSPSITKVYVI
                     ASEQTAKAVTNFVGELGAAYLRLFAKRYPLVAEKGQLTFLKEQMDGFSKERDRMVELM
                     KQFNLDGKLDQRQWDVINKNFQFESNRVTETIKQHATRARILYASQIEYMKECIAEHN
                     RLSRLLVPVLISVRAELALPINETAYMEVIEESIRKQEVSLKEFIQNVKNLAADEQGS
                     TENNVD"
     gene            complement(2062..2499)
                     /gene="rnhA"
                     /locus_tag="AC16FV_11860"
     CDS             complement(2062..2499)
                     /gene="rnhA"
                     /locus_tag="AC16FV_11860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR2850779.1"
                     /translation="MDKVTIFTDGACRGNPGPGGWGAILRKDDHEKELYGAERETTNN
                     RMELMAAIQALEALKRPCEVTLTTDSQYVKKGITEWLASWKRRGWKTADKKPVKNQDL
                     WQRLDEAAHKHKIHWKWIKGHSGHEENERADQLANRAIEEMLL"
BASE COUNT          530 a          777 c          737 g          655 t
ORIGIN      
        1 tcgtagtctt gccggcgccg ttgccgccga gcagcgcggt ggtggaccgc gccgtgacat
       61 cgaagctgat gtcatcgaca gcgatgacgc tgtcgaaatg cttgctgacg tgggaaatgg
      121 aaacaacaga tgcagacatg aggcgcgcag attagcgcag cgatgcaagc ttgtcagctt
      181 actgttgtag cccgggtgaa ggcccgcgca gggccggaac ccggggaaca tcaacagcac
      241 cccggattcc gcttcgctac atccgggctg catctgtctg acctgccgtc attcccgcga
      301 aagcgggaat ccaggaatta ttagcgtagg ggcgccctgc gcgccgatca accaaatggc
      361 gcgtggtgta cgcgccctac ggcttaaagt ctctaagagg ctggctccag ctttttccac
      421 aaacacttgc caccgctttt cttgtccagc tcctccagcc acgcctgatg cgccgccagc
      481 tcctcggcgg ttgcggcgat caccggcagt ttcggccggt tgggatcgag gcggcggata
      541 tccgagggca cggcggcgat gttgccagtc gtggcgcctc gcaagtggct gtgcgtgtcc
      601 tcgaacaagc cggtctgccc gccggtcatg gcgagataca cgtccgccag gatctcggcg
      661 tcgagcagcg cgccgtgcag ggtgcggtgc gaattgtcca cgttgtagcg ccggcacagc
      721 gaatcgaggt cgttcttctg gcccgggtgc atggagcgcg ccaacttcaa ggtatccacc
      781 acggaacaga tttcctcgac gcgcaccggc ttcccgccgt tcgccacgcg agacaattca
      841 ctattaagga atccgacgtc gaacggcgcg ttgtggatga tcagctccgc gtcctgaata
      901 aaatcaagta gactctgcgc gatgtcggcg aacttcggct tgctcgccaa ctgctcattg
      961 gtgatcccgt gcacctccat cgcccccgcg tcgatctcac gttccgggtt gatgtactgc
     1021 tgatagcggt tgttgctcgc tcgccggttg atcagctcca cacagccgat ttcaataatc
     1081 cggtggccct ccgcaggctc caggccggtg gtttcagtat cgagaattat ctggcgcatg
     1141 ggtgtcacat tatatccatt ctttaatcaa cattgttttc ggtagaacct tgttcgtcgg
     1201 ccgcgagatt tttcacattt tggataaatt cctttaggct gacctcctgc ttcctaatac
     1261 tttcctcaat aacttccatg tatgcagtct cgttgatcgg taacgcaagc tctgctctta
     1321 cagagatgag taccggtacc aaaagtcggc tgagcctgtt gtgttctgcg atgcactctt
     1381 tcatgtactc aatctggctc gcgtataaaa tgcgagcacg tgtagcatgc tgttttattg
     1441 tttcagtgac gcgattagac tcaaactgga aattcttatt aatcacatcc cactgtcgtt
     1501 gatctaactt gccatcgagg ttgaattgtt tcattagctc gaccatacgg tcgcgttctt
     1561 tagaaaaacc atccatctgt tccttcagaa aagtgagttg tcctttctct gccactagtg
     1621 ggtaacgttt agcgaaaagg cggagataag ctgcgcccag ctcgccaaca aaattcgtga
     1681 cagcttttgc cgtctgttcg ctagcgatta cgtaaacttt ggtaattgag ggtgacttgt
     1741 cgatgtacgc ctcagtgagc ttatcgtgcg gtatgtctag gtttgaaaac tggctaacgg
     1801 tgattagtcc tgccgaaacg gcctctgcgg ccgcaagata aacatccctt ctcagggata
     1861 gctcccgctc tcgagatttc atatctctat catgagcaag ctgcgcatgt aagcgccggt
     1921 cgtttgcacg gtttgttaac gcgacgccgc cgagcgagaa aaatgcacca atcacaacac
     1981 cccaaaaggt ggctggaatc ttttcgatca aatttaatat ttcggcaagc ataaaattaa
     2041 atccgcaaaa gaaaggcaaa ctcacaacaa catttcttca atcgcccgat tggcgagctg
     2101 atcagcgcgc tcgttctcct catgtccgct gtgccccttg atccacttcc agtgaatctt
     2161 gtgcttgtgc gcagcttcgt cgaggcgctg ccataagtcc tggtttttca ccggcttctt
     2221 gtcggccgtc ttccagccgc ggcgcttcca gcttgcgagc cattcggtga tgcctttctt
     2281 gacgtattgc gagtcggtgg tgagcgtcac ttcgcacggg cgcttgagcg cctcgagcgc
     2341 ctgaatcgcg gccatgagct ccatgcggtt gttggtggtc tcgcgctcgg cgccgtagag
     2401 ttccttttcg tggtcgtcct tgcgcaggat cgcgccccag cccccgggtc cgggattgcc
     2461 gcggcaagcg ccgtcagtga agatcgtgac tttatccaag cgagatgtgg cgaatagtta
     2521 ttattgttta cggaatggca ggatcatgcc gcgcgcggcg ggttgggtgg cgcccgcggc
     2581 gccgacggcg cgctgggttt tccagctcac cggcagcggc gtgacgccga ccacgcgctt
     2641 cttcgccacg acgagataca cccccgccat catcggccac cagcggtcgc ccatcctgt
//
LOCUS       DBNGZE010000456         2024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1868915, whole genome shotgun sequence.
ACCESSION   DBNGZE010000456 DBNGZE010000000
VERSION     DBNGZE010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1868915"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="AC16FV_11865"
     CDS             complement(<1..635)
                     /locus_tag="AC16FV_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850780.1"
                     /translation="MKMKIFKLLTLLVLAAPLVHGYGQCSIAHAAGSGGFPSKPTFAK
                     ITIKPNETNGAVKVYQRDGNDLGFVLFGSTGATHPAFTNSVEGISTGDSAYLTSSIYW
                     NGSGWIASEGTAGNGFASMLILNNAGCLVSFQSATGLTGGAAAVPAEVLCIKRDGSMS
                     SSKACASGFTHITPNFCSRGTNSSNYVALVRDTCTAVARPSADTVAVLVRAT"
     gene            complement(632..853)
                     /locus_tag="AC16FV_11870"
     CDS             complement(632..853)
                     /locus_tag="AC16FV_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850781.1"
                     /translation="MKKLIETAAAPAPAGDKSETDRVFETELTAIVAVKVPGGYVVFD
                     NPDEAQEFVKNPVVPVDIVRVPIIPEVAK"
     gene            complement(853..1404)
                     /locus_tag="AC16FV_11875"
     CDS             complement(853..1404)
                     /locus_tag="AC16FV_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850782.1"
                     /translation="MATHDYKITIKANDRQPVSGPVNFIRLHASTGNLKIDIEGKVTE
                     LAVGQAIVLDELVDLFHVVNESGSDINATFKLGAGARVTDNNLSGTVVLNKATGAMDT
                     ADQSIANGATVTITPSATARALIIYFHPNNTGNLRTGPAAAAARGRIGQPGIEHVWNT
                     TNAIKVHNDSGAAQTFGYAEETD"
     gene            complement(1391..1858)
                     /locus_tag="AC16FV_11880"
     CDS             complement(1391..1858)
                     /locus_tag="AC16FV_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850783.1"
                     /translation="MGFFDSSSSSETKNYTQTETTTAGAESGIAAAGGSSVTILDPGA
                     VKLAGDVASNTIEASGASLRRSLDFGEVSLSGALDFALDVVDLAGKQSADALKIYSAK
                     AQEDLQAAVTLSKGQTQQVTENITKWSLTIVGVLAAAAALMFVFNPGAKRGHA"
BASE COUNT          408 a          601 c          596 g          419 t
ORIGIN      
        1 gtggccctga ccagcaccgc cacggtatcg gcagacggcc gcgcgacggc agtgcaggta
       61 tcgcgcacta aagcgacgta gttgctggaa ttggtcccgc gcgaacaaaa attcggcgtg
      121 atgtgcgtaa agcccgaggc gcaggctttc gaggacgaca ttgagccgtc gcgtttaatg
      181 cagagcacct ccgctgggac cgccgccgcg ccgcctgtta aacctgtcgc cgattggaac
      241 gaaaccaaac acccggcatt atttaatatc agcatcgacg caaaaccgtt gccggcggtt
      301 ccttcactcg ctatccatcc gctgccgttc caataaatac tggacgtcaa ataggcggaa
      361 tcgccagttg aaattccctc gaccgaattc gtgaaggccg gatgagtggc ccccgtcgaa
      421 ccgaacaaca caaaacctag atcgtttccg tcgcgctgat agactttgac cgcgccgttg
      481 gtttcattcg gtttgatcgt gatcttggcg aatgttggct tgctaggaaa gccacctgag
      541 ccggcggcgt gcgcgatgga gcattgtcca tatccatgca ccaacggcgc ggccagcacc
      601 agcagcgtca acaacttaaa aattttcatc ttcattttgc aacctccggg ataatcggca
      661 cgcgcacgat gtcgaccggc acgaccggat ttttgacaaa ctcctgtgcc tcgtcgggat
      721 tatcgaaaac gacgtaacca cccggaacct tgaccgccac tatcgcggtc agttcggttt
      781 caaacacgcg gtcggtttcg cttttgtcgc cggccggcgc cggcgcggcg gccgtctcaa
      841 tcagcttttt catcagtcgg tctcctcggc gtagccaaag gtttgcgccg cgccgctgtc
      901 gttatgcacc ttgatggcat tggtcgtgtt ccacacatgc tcgatgccgg gttgaccgat
      961 gcggccgcgc gcggcggcgg cggccgggcc ggtgcgcagg ttgccggtgt tgttcggatg
     1021 aaaataaata atcagcgcac gcgccgtcgc gctcggcgtg atcgtcacgg tcgcgccgtt
     1081 ggcgatcgac tggtcggcgg tatccatcgc gcccgtagcc ttgttgagca ccacagtgcc
     1141 cgacagattg ttgtcagtga cccgcgcgcc ggcgccgagc ttgaacgtgg cgttgatatc
     1201 ggatccggac tcattgacga cgtggaacag atcgacgagc tcatcgagca cgatggcctg
     1261 gccgacggcc agctcggtga ccttaccctc gatgtcgatc ttgagattgc cagttgacgc
     1321 gtgcaggcgg atgaaattca ccggccccga gaccggctgg cggtcattcg cttttatggt
     1381 gatcttgtaa tcatgcgtgg ccacgtttgg ctcctggatt aaaaacgaac atgagcgcgg
     1441 cggcggcggc gaggacgccg acgatcgtga gcgaccattt tgtgatgttc tcggtcacct
     1501 gttgcgtctg acctttcgag agcgtgaccg ccgcctgcag atcctcctgc gccttcgcgg
     1561 aataaatttt cagggcatcg gccgattgct taccggcgag atccaccacg tcaagtgcga
     1621 agtcgagcgc gccgctgagc gacacctcgc cgaaatcgag actccttcgc aggctcgcgc
     1681 cagaggcctc gatggtattg ctggccacgt cgccagcaag tttgaccgca cctggatcca
     1741 ggatcgtgac ggaggatccg ccggcggcgg cgatgccact ctcggcgccg gcggtggtcg
     1801 tttccgtctg cgtataattt ttggtctcag aactggagct ggaatcaaag aagcccatgc
     1861 gtcaccctct cgatttcgcg cgtggccaca gcgcgacaac caatgcgccg agagcgagaa
     1921 agaaggacga gaacaggatt ccaatgggat cattcttagc cagtaaagaa atggcgatgc
     1981 tgtcgaaacc gtacccgctg ctgaagaata cctgcatgca gcgg
//
LOCUS       DBNGZE010000457         4073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_897017, whole genome shotgun sequence.
ACCESSION   DBNGZE010000457 DBNGZE010000000
VERSION     DBNGZE010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_897017"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1700)
                     /locus_tag="AC16FV_11885"
     CDS             complement(72..1700)
                     /locus_tag="AC16FV_11885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2850784.1"
                     /translation="MLFLIGFIVAIAISMVIIPLMARLAPHIGMIDRPDPRKVHAVPI
                     PRAGGVGIVLGALVPVAMWLPLDQLSGAYLFGALILLAFGVWDDIQELGHYVKFIGQF
                     AAVIAVVYYGNLYVTRLPFMEAELTETTGKVFTVFAMIGMINAVNHSDGLDGLAGGMT
                     LLSLGCIAYLASMADADGSFVVLIALAVLGGVVGFLRYNTYPARVFMGDGGSQFLGFT
                     AAFLTVYMIEKVNPALSRALPALILGLPIMDILAVFAQRAYHGMNWFRASKNHVHHRL
                     LALGFDHYESVVIIYSIQTLFVISAIFFCYESDILILSIYFGVCAALFAFLTVAEHRR
                     WRAHRAGRASSFARMISSIKANHFFIAAPIQFIGVAIPVLFIAVSLLAKQVPRDFGII
                     SAVMAGALVLYFVARISVDSIIAQAVHYVTAAFVVYLETRHLGENIPGFDAMQAVYFG
                     ILIAAIGLAIRYGEKSDFRTTPMDYLVIIIALTASFVMQNTPEMAEIGLMAAKLIVVF
                     YACELIVRRVRRKWNALNIATFVALTVLAYRGLV"
     gene            complement(2151..3059)
                     /locus_tag="AC16FV_11890"
     CDS             complement(2151..3059)
                     /locus_tag="AC16FV_11890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2850785.1"
                     /translation="MNRRYVLISPCRNEADFMRQTLDTVIAQSIRPAKWIIVDDGSTD
                     DTPKILEEYSRRHDWIEVVTRTDRGHRAVGPGVIEAFYAGYEAIEPDKYDYLCKLDLD
                     LRLPPRYFEILMERMEADPRIATCSGKSYFEKEGRLVSERIGDETSLGMTKFYRVSCF
                     REIGGFVREVMWDGIDCHRCRMMGWIACSWDEPELRFVHLRPMGSSHKGIYTGRMRHG
                     YGQYFMGTGFVYMMASAAHRMIDKPYVLGGLAMMWGWLKSALQGKPRYDDPEFRRFLR
                     RYQWRALLVGKKRAIEGIHRIKGIDG"
     gene            complement(3056..>4073)
                     /locus_tag="AC16FV_11895"
     CDS             complement(3056..>4073)
                     /locus_tag="AC16FV_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2850786.1"
                     /translation="AWKALRWLVADITELGLMRLQSWKLVFQFFAAVKVARLLKQHGC
                     VHLHVHFAHVPAQIAMYASALSGIPFTVMAHANDIFESDLLLPQKAERALKMLTISEY
                     NRSHLEKMGVAADKLAVVRCGVSLGGRLTAPDFKRRDRYRIGALARLVDKKGMDVLIR
                     AVAELRSRPYRIELSIAGDGPLRSELETLVRGLNLKDTVRFEGSMAHSQVTDWMRSLD
                     AFVLACRKDSHGDMDGIPVVLMEAMSQYVPVVSTRLSGIPELVLHEETGLLADPGDHL
                     SLAAQIDRLLESAELRSRLAARAIRHVRREFGQDVNLDRLLGHLGFSGFVSAVNWNTD
                     RGQS"
BASE COUNT          995 a         1215 c         1050 g          813 t
ORIGIN      
        1 agacctgcat cgtggcaatt ggacttagac atatctcgta tcgccgggac aaatttcatg
       61 ggtagcgacg tttacacaag tccgcgatat gccagcaccg tcagcgcaac aaatgtcgcg
      121 atgttcaggg cgttccattt gcggcgcacg cgcctcacga taagctcgca ggcataaaag
      181 accacaataa gcttcgccgc catcaggccg atttccgcca tttcgggcgt gttctgcatg
      241 acgaagctgg ccgtcagagc gataataatc accaggtaat ccatgggtgt ggttctgaaa
      301 tccgattttt ccccgtaacg gattgccagc ccaatagctg cgatcagaat cccgaaatag
      361 acggcctgca tggcgtcgaa accaggaatg ttttcaccaa ggtgacgcgt ctccagataa
      421 accacgaagg cggccgtcac gtaatgaacc gcctgggcaa tgatggaatc gacagaaatc
      481 ctggcgacga aatacagaac gagcgcccct gccatcaccg ccgatattat cccgaaatct
      541 ctggggactt gttttgccag caggctcacg gcaatgaaca gaacgggaat ggcgactccg
      601 atgaattgaa tgggcgcggc gataaaaaaa tggttggcct tgatcgaact tatcatccta
      661 gcaaaactag aagcgcggcc ggcacggtgg gctcgccagc gccggtgttc cgctacggtc
      721 aaaaacgcga acaatgccgc gcatacgccg aaatatattg aaagaatcag gatatctgac
      781 tcgtagcaaa aaaatatcgc gctgatgaca aataatgtct ggatggaata gatgatgacc
      841 acagattcat agtgatcaaa acccagcgcc aacagccggt ggtgcacgtg atttttggag
      901 gcgcgaaacc agttcattcc gtgatacgcg cgctgggcaa aaaccgccag gatatccata
      961 atcggcaaac ctagaatcag cgccggcaac gcccggctaa gtgcgggatt caccttctcg
     1021 atcatgtaaa cagtaagaaa cgcagctgtg aatccgagga actgactgcc tccgtcgccc
     1081 ataaaaaccc tcgccggata ggtgttatag cgcaagaagc ccacgacgcc gcccaggaca
     1141 gcgagcgcga tcaacacgac aaatgaaccg tcggcgtcgg ccatcgaggc aaggtaggcg
     1201 atacaaccca ggctcaacaa ggtcattccg ccggcgagcc cgtcaaggcc gtcggaatgg
     1261 ttcacggcat ttatcattcc gatcatggca aacaccgtga agaccttgcc ggtggtctct
     1321 gtcagttccg cctccatgaa gggcaaacgc gtgacgtaca gattcccgta atagaccacg
     1381 gcgatcacgg cggcaaactg gccgatgaat ttgacgtaat gaccgagttc ctggatatcg
     1441 tcccacacgc caaaagcaag aaggatcagt gcgccaaaga gatacgcacc tgacaactga
     1501 tccaacggca gccacatggc aacaggcacc aaagcgccca acacgatccc cacaccgccg
     1561 gcgcgcggga tcggaaccgc atgcaccttg cggggatcgg gacgatcaat cattccgata
     1621 tgtggcgcga ggcgcgccat gagcggaatg atgaccatgc tgatagcaat ggctacgatg
     1681 aacccgatca ggaagagcat ctgtctctgt atccctaatt atttaccctg catagccggg
     1741 cttgtgtcga tggatgccca tctgatcgaa gaggcgctgg cattacttat tgttgtcctg
     1801 tctcataaca acaggatgct gctaatgatc aaggggatat atttataaaa ttatgacaaa
     1861 ttatactaca tcgcaggtag gccagcgccc acccggaata agcggcggag gctgtttaaa
     1921 caccagatgg ccacgcacac gtcatctgca tgctaaccgc tcccggcggg tctttcaggg
     1981 gcgtcctatc tccactcttc aattcccctg aaaataggca agaaagctaa gattgggcat
     2041 cgacaggagg catcgttacc aatggtaggt gacatcacgg atacggttgg ccctcccttc
     2101 catggttgcg gaatcggatg acatgccgag catagggcgc cctgttaata tcaaccgtca
     2161 atacccttta tcctgtgaat cccctcgatt gcccgcttct tgcccaccag cagtgcgcgc
     2221 cactggtagc ggcgcaggaa tctgcggaat tccggatcgt catagcgcgg cttgccctgc
     2281 aacgcgctct tcaaccagcc ccacatcatc gccagcccgc ccaagacata gggcttatcg
     2341 atcatccgat gggcagcact ggccatcatg taaacaaacc cggtccccat gaagtactgg
     2401 ccgtagccat ggcgcatgcg gccggtataa atgcctttgt gactggagcc catgggccgc
     2461 aaatgcacga agcgcagctc aggttcatcc cagctgcacg cgatccaccc catcatccga
     2521 cagcggtgac aatcaatacc atcccacatg acctcgcgca caaagccgcc gatctcccgg
     2581 aaacaggaaa cgcgatagaa ttttgtcatt ccaagagaag tttcatcgcc aatccgttcg
     2641 ctgaccaatc tgccttcttt ttcgaaatat gacttgccgc tgcacgtggc gatcctcggg
     2701 tcggcctcca ttcgctccat caggatctcg aaataacgtg gaggcagccg caaatcgaga
     2761 tcgagcttgc acaggtaatc gtacttgtcc ggctcgatag cttcgtatcc ggcatagaag
     2821 gcctcgatca cccccggccc cacggcgcgg tgccccctgt ccgtccgcgt gaccacttcg
     2881 atccagtcgt gtctgcggct gtactcttcg agaattttcg gcgtgtcgtc cgtcgagccg
     2941 tcatctacaa tgatccactt ggccggccgg atcgactggg cgatgaccgt gtcgagagtc
     3001 tgccgcatga agtccgcctc attccggcat ggcgatatca gtacatagcg tcggttcatg
     3061 attgccccct gtcagtattc cagttcacgg ctgatacaaa accggaaaac ccgaggtgtc
     3121 ccagcagtcg gtccagattg acgtcctggc caaactcccg gcgcacgtgc ctgatcgccc
     3181 gggccgccag gcgggaccgc agttcagctg attccaacag ccggtcaatc tgtgccgcca
     3241 gactcaagtg atcgccagga tcggccagca atccggtttc ctcgtgtaat acgagttcgg
     3301 ggattccgga aagccgggtt gataccaccg gcacgtattg cgacatggcc tccatcagca
     3361 ccaccggaat accgtccatg tctccgtggc tgtccttccg gcaagccagt acgaaggcat
     3421 ccagactcct catccagtca gtcacctggc tgtgggccat gctgccttcg aagcgcaccg
     3481 tatccttaag gttcaagcct cgcaccaggg tttccagttc actgcgcaag ggcccgtcgc
     3541 cggcgatact cagttcgatc cggtacggac gactccgcag ttcagccacg gcccggatca
     3601 acacgtccat gcctttctta tcgaccaggc gcgccagagc gccaatacga tatctatcgc
     3661 ggcgcttgaa atccggcgcc gtcagacgcc ctccaaggct gacgccacaa cgcaccaccg
     3721 cgagcttgtc ggcggcaacg cccatcttct ccagatggga gcgattgtat tcggagatag
     3781 tcagcatctt caaagctctc tccgctttct gcggcagcag caggtcagac tcgaagatgt
     3841 catttgcgtg tgccatgaca gtgaatggaa taccgctcag tgccgaggca tacatcgcaa
     3901 tctgcgccgg cacatgggca aaatggacat gcaggtgtac acagccatgc tgtttcagca
     3961 accgggcaac cttgaccgca gcaaagaatt ggaataccag cttccagctt tgcagcctca
     4021 tcaatcccag ttcggtgatg tcagcgacaa gccagcgtaa cgccttccat gca
//
LOCUS       DBNGZE010000458         5058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1036446, whole genome shotgun sequence.
ACCESSION   DBNGZE010000458 DBNGZE010000000
VERSION     DBNGZE010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1036446"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="AC16FV_11900"
     CDS             <1..301
                     /locus_tag="AC16FV_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2850787.1"
                     /translation="IAPKMNPTRRFAQMFWKNITKGGRVNELKLSISLYFMDGFASGI
                     KKGLEMLPVGLGMLKTKRLNPFGLVSGGTVKGAADLHKMLKKAKEIEDKRKGFAA"
     gene            320..1222
                     /locus_tag="AC16FV_11905"
     CDS             320..1222
                     /locus_tag="AC16FV_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR2850788.1"
                     /translation="MARKEYSFYPGCSSERNASASNYMVSVESMCKTLDIKLNEIPDW
                     NCCGASIGYAEGGELPRHVMNARNIALSEKHHGDQDIVATCAACWLGAKESQERINHS
                     STLMGDINDILKDAGLKYEGKQKIRHMVEVLIEDLGYEELGKPVKKPLEGIKIAGYVG
                     CQTNRPFGVDGESFENPKYLDKLVETVGGTALDKYEQKVTCCGGALAFSEPEKSQAQI
                     KKIIESAYDHGADMIVTPCPVCQMNVEVYQGQINKKYGTKFNIPVTYYSQLMTVAYGG
                     SAKEAGLDGNIIRATKLEEIAAKK"
     gene            1372..2775
                     /locus_tag="AC16FV_11910"
     CDS             1372..2775
                     /locus_tag="AC16FV_11910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR2850789.1"
                     /translation="MQSSDIKTQILSHSPMVTPGTAQDIAEALAARYEVLDSIGDGGM
                     GMVYRVRDRETSEILALKLLRPEIARDPAMMERFKNEIRLAHRITHKNVCRIYDFNRV
                     DELAYFTMEYVDGESLRAYLKRAGKLTPERTIDLARQITAGLGEAHAQGVIHRDLKPE
                     NVMLGRDGLVKLLDFGIARAVGSDTATARTIMGTPEYMAPEQSQGKAVDQRVDLYALG
                     LILYECLAGRRAFSGATPVEVALKQLKERPVPPRKFLPATPPYLETIVMRCLEKEPTQ
                     RYASATEIQRALVQPGATSQPAPRLRKTAVIAIVASLAALGAYKMVNRQDTLSASVPV
                     QQAPVNLTDQQQIPEELVKLFEAAKQGDAAAQERVGRLYLDGPARARDERKAFFWTRR
                     AALSGDGNAQFTLAQMYESGRGAPQNLVGAYVWYNIALSSGNESARVPFERLMGQISS
                     DELTEAEQQLHKLRRDR"
     gene            complement(2798..4120)
                     /locus_tag="AC16FV_11915"
     CDS             complement(2798..4120)
                     /locus_tag="AC16FV_11915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR2850790.1"
                     /translation="MLRPSRMGAGEGPHRMNPFSFTHLPLRTRFLVAMGIVFVPLVVV
                     ALGSSLFLGHAASTLNRVVEQSVYKLQTTSRLQNQIRKTHALAKDYAVTPRYTLQTQF
                     ESEARAVEDIFATILREPFVNPQEQILLANVRREWNDSVVLANTIFSANASRASLFQL
                     DQRVNQIMFALDQVYDAYYTEIGVQRAQINESEKRFLLIISGAVGLGLLIAIVGAVVL
                     TRSVLGPLREFEKGMTRFSNENLSYRLTLDSRDEIGQLAREFNAMAERLMQHQHKLED
                     LSIRDGLTGIYNRRELEKRLHQECQRARRYHRPLSVLMLDIDHFKNVNDRYGHQAGDE
                     VLITVADLIQLNVRPVDVVCRYGGEELAVILPETNAEGASTAAERIRSTVEDSITATP
                     QGDMVHVTVSIGLATFPRDGDTAASLIHAADQALYVAKQEGRNLVRSH"
     gene            4329..4544
                     /locus_tag="AC16FV_11920"
     CDS             4329..4544
                     /locus_tag="AC16FV_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850791.1"
                     /translation="MRVSTTDPISGADVKNPERSPFVVEGEGEGALKIYFENEDNKSA
                     YLEIQTEHPGKDFTTNLDNPARMGTER"
     gene            complement(4590..>5058)
                     /locus_tag="AC16FV_11925"
     CDS             complement(4590..>5058)
                     /locus_tag="AC16FV_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850792.1"
                     /translation="AGAHGADLTALVRRAQTVIIPVLPSPTDIRAAAHFIHELLLVGK
                     IVRDETRLAVVANRVRENSSIQNTMESVLGSMNINYSTLNTQIYHNLERFLSRLKIPF
                     ITTLRESQNYLVADARGLGIFDLTRSQVVRDIEQWGPLLKWLESKRSIPEPAT"
BASE COUNT         1059 a         1492 c         1579 g          928 t
ORIGIN      
        1 catcgcgccg aagatgaacc cgacgcgccg cttcgcgcag atgttctgga agaacatcac
       61 caagggcggc cgcgtcaatg aactgaaact gtccatcagt ctgtacttca tggacggctt
      121 cgccagcggc atcaagaagg gcttagaaat gctgccggtg ggtctcggca tgctcaagac
      181 caagcgcctg aacccgttcg gcctggtcag cggcggcacg gtcaagggcg cggccgatct
      241 gcacaagatg ctcaaaaagg ccaaagagat cgaagacaag cgtaagggtt ttgccgctta
      301 agcggcggcg aggtaaatca tggcacgtaa agaatattcc ttttatccgg gctgttcctc
      361 cgagcgcaac gcctcggcct cgaactacat ggtctcggtc gagtccatgt gcaagacgct
      421 cgacatcaag ctcaacgaga ttcccgactg gaattgttgc ggcgcctcca tcggctacgc
      481 cgaaggcggt gagctgccgc gccacgtcat gaacgcgcgc aacatcgcgc tgtccgagaa
      541 gcaccatggc gatcaggaca tcgtcgccac ctgcgccgcc tgctggctgg gcgccaaaga
      601 gtcgcaggag cgtatcaatc attcctcgac cctgatgggc gacatcaacg atatcctcaa
      661 ggacgctggc ctgaagtacg aaggcaaaca gaagatccgt cacatggtcg aggtgctgat
      721 cgaggacttg ggctacgagg agctcggcaa gccggtgaaa aagcctctcg aaggcatcaa
      781 gattgcgggc tacgtcggct gccagaccaa ccgcccgttc ggcgtggatg gcgaatcgtt
      841 cgagaacccg aagtacctcg acaagctggt cgagaccgtc ggcggtaccg cgctcgacaa
      901 gtacgagcag aaggtcacct gctgcggcgg tgcgctcgcg ttctccgaac cggagaagtc
      961 ccaggcgcag atcaagaaga tcattgagtc ggcctacgac cacggcgccg acatgatcgt
     1021 cacgccctgc ccggtatgcc agatgaacgt cgaggtgtat cagggccaga tcaacaagaa
     1081 gtacggcacc aagttcaata tcccggtgac ctactattcc cagctcatga ccgtggccta
     1141 cggcggcagc gccaaggaag ccgggctcga tggcaacatt atccgcgcca ccaagcttga
     1201 ggagattgcg gctaagaaat aaagccgatc agtcgttata gacaaaaaag cccgcgaaag
     1261 cgggcttttt tgtgggccgg accggggtgt cggcaggggt atatgcccga gtcttgccta
     1321 tacttgtgaa aatgagcgcc gggcatgctg gcgcccccat gagcgcctga catgcaatcg
     1381 tccgacatta aaacccagat tctttcgcac tcgccgatgg tgacgcccgg caccgcgcag
     1441 gatatcgccg aggcgctggc ggcgcgttac gaagtcctgg attccatcgg tgatggcggc
     1501 atgggcatgg tataccgtgt gcgcgaccgc gagaccagcg agatactggc gctgaaatta
     1561 ttgcggccgg agatcgcgcg cgatccggcc atgatggaac gcttcaagaa cgaaatacgc
     1621 ctggcccatc gcatcacgca caagaacgtc tgccgcattt acgatttcaa ccgcgtcgat
     1681 gaactcgcct acttcaccat ggaatacgtg gacggcgaga gcctgcgcgc atacctcaaa
     1741 cgcgcgggca agctgacgcc cgagcgcacc atcgatctcg cgcgccagat caccgccggt
     1801 ctgggcgagg cgcatgccca aggtgttata caccgcgact tgaagccgga gaacgtcatg
     1861 ctcggtcgcg acggactggt gaagctgctg gatttcggca tcgcccgcgc cgtgggcagc
     1921 gacaccgcca cggcacgcac catcatgggc acgcccgaat acatggcccc ggaacaatcg
     1981 cagggcaagg cggtggatca gcgcgtggat ctgtatgcgc tgggactcat tctctacgaa
     2041 tgcctggccg gccggcgcgc gttttccggc gccaccccgg tggaggtcgc gctcaaacag
     2101 ttgaaagaac ggcccgtgcc gcccagaaaa tttctgcccg ccacgccgcc atatcttgaa
     2161 actatcgtga tgcgttgcct ggaaaaagaa ccgacccagc gttatgccag cgccacggaa
     2221 attcagcgcg cgctggttca gcctggcgcc acatcgcaac cggcacctcg cctgcgcaag
     2281 accgctgtta ttgcgatcgt cgcatcgctg gctgccttgg gcgcttacaa aatggtgaac
     2341 cggcaggaca cattatccgc atcggttccg gtacagcagg ccccggtcaa tttgactgat
     2401 caacagcaga taccggaaga actggtcaag ctgttcgagg cggcgaaaca gggcgacgcc
     2461 gcggcacagg agcgtgtcgg ccggttgtat cttgatggac ccgcgcgcgc ccgcgatgag
     2521 cgcaaggcct ttttctggac gcgtcgtgcc gcgctcagcg gcgatggcaa cgcgcagttc
     2581 acgctggcgc agatgtatga aagcggtcgc ggcgcgccgc aaaatctcgt tggcgcctat
     2641 gtctggtaca acattgcgct gagttccggc aacgaatcgg cgcgcgtccc gtttgagcga
     2701 ctgatgggcc agatttccag tgatgaactg accgaggcgg aacagcagtt acataaactg
     2761 cgaagagatc ggtaacctcg ggcaggcgac gcgcggtcta gtggctgcgc acgagattgc
     2821 gcccttcctg cttggcgaca taaagcgcct ggtcggcggc atggatgagg ctggcggcgg
     2881 tgtcgccatc gcgcggaaag gtggcgaggc cgatgctgac cgtgacatgc accatgtcgc
     2941 cctggggcgt cgcggtgatg gaatcctcca ccgtgctgcg tatgcgttcc gccgccgtgc
     3001 ttgccccttc cgcattcgtc tcgggcagaa tcacggccag ttcctccccg ccgtagcggc
     3061 acaccacgtc caccggccgt acattcagct gaatcaggtc cgcgacggta atcagcacct
     3121 cgtcgcccgc ctgatgtccg tagcgatcat tgacgttctt gaaatggtcg atgtccagca
     3181 tcagcaccga gagtggccgg tggtaacggc gggcgcgctg gcattcctgg tgcaggcgtt
     3241 tttcgagttc ccggcggttg tagattccgg tgagaccatc gcgaatcgac aggtcctcca
     3301 gcttgtgctg atgctgcatc aggcgttcgg ccatggcgtt gaattcgcgc gcgagctggc
     3361 cgatttcatc gcggctgtcc agcgtcagac ggtaggacag gttttcattg gaaaagcggg
     3421 tcatgccctt ctcgaactcg cgcaaggggc ccagtaccga acgggtgagc accacggccc
     3481 cgacgatggc gatcagcaaa cccagcccca cggcgccgga gatgatcagc aggaaacgtt
     3541 tttccgattc gttgatttga gcgcgctgca cgccgatctc ggtgtagtag gcgtcataaa
     3601 cctgatcgag cgcgaacatg atctggttca cgcgctggtc cagctggaac agggatgccc
     3661 tggaggcgtt cgctgaaaaa atggtgttgg ccagcaccac gctgtcattc cattcgcgcc
     3721 ggacgttggc gagcaggatt tgttcttgcg ggttgacgaa gggctcgcgc agtatcgtgg
     3781 cgaagatgtc ctccacggcc cgcgcctcgg actcgaactg ggtctggagg gtgtagcgcg
     3841 gcgtgaccgc gtagtccttg gccagcgcgt gggtcttgcg aatctggttc tgcaggcgcg
     3901 aggtcgtctg gagtttgtaa accgactgct cgaccacgcg gttcagcgtg ctggcggcgt
     3961 gtcccagaaa gaggctggag ccgagcgcca ccacgacgag cggaacgaaa acgattccca
     4021 tggcgaccag aaaacgggtc cgcagcggca gatgcgtgaa ggagaaagga ttcatccggt
     4081 gcggtccctc gcctgcgccc atgcgggatg gccgcagcat ttgctggttc tttgtaaagt
     4141 atagtccggc gcccggtttt cccggctaag cgtcggaaaa tccgcagaaa atgcccgtcg
     4201 tcgtgtgccg aagcaaggcg gcgaataacc gggtctacgt cattgacaga cagaggcgcc
     4261 gggagtagtt ataaaagggt cgaggcatat tgcctgcatg tattgttacc aaccgtaagg
     4321 agcatgccat gcgtgtatca acaaccgatc ccatctccgg cgccgatgtg aaaaatcccg
     4381 agaggagccc gttcgttgtc gagggagaag gcgagggcgc gctcaagatt tatttcgaga
     4441 acgaggacaa caagagcgcc tatctcgaaa tccagaccga gcatccgggc aaggatttca
     4501 cgaccaacct ggacaacccg gcccgaatgg ggacggagcg ctgaaccggc gttgcggaga
     4561 ccgcggcgct gaaagacggt gggagtcggc tatgtcgctg gctccggaat gctgcgcttg
     4621 ctctcgagcc acttgagcag cggcccccac tgctcgatgt cgcgcaccac ctgtgagcgc
     4681 gtcaggtcga atattcccaa tccgcgcgca tcggccacca gataattctg gctttcgcgc
     4741 agggtggtga tgaagggaat cttgagacgc gatagaaacc gttcgagatt gtggtaaatc
     4801 tgggtgttga gcgtggaata attgatgttc atgctgccca gaacgctctc catcgtgttc
     4861 tggatcgagc tgttctcgcg cacgcgattg gcgaccaccg ccaggcgcgt ctcgtcccgc
     4921 acgattttcc cgaccagcag cagctcgtga atgaaatgcg ccgccgcgcg gatgtcggtc
     4981 ggcgagggca gcaccgggat gatcacggtc tgggcgcggc gcaccagcgc cgtgagatcc
     5041 gcgccatggg ccccggcc
//
LOCUS       DBNGZE010000459         4177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1730713, whole genome shotgun sequence.
ACCESSION   DBNGZE010000459 DBNGZE010000000
VERSION     DBNGZE010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1730713"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="AC16FV_11930"
     CDS             complement(<1..717)
                     /locus_tag="AC16FV_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025695.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4393 domain-containing protein"
                     /protein_id="HIR2850793.1"
                     /translation="MPNEILPILKEVAQVPGLLKQIYGDLARPGVTQVGKALGAVLGL
                     GNTLLWPLALLNERSKIALEKNLEKYREQLSTASPEKIVEVSPEVGVPITEKLTYVTD
                     DQLSDLYVNLLAKASVFESTHTAHPSFVNVINNLSPDEALLLQQIRGDIPFLEARIIK
                     GIKNGVKEWIPLGQLLTGLETKTKLSFPQNIVAYMSNFEGLGLIEIRHDYFMVAQGQY
                     EELEKIYRPELDKISNLMKIG"
     gene            complement(822..2309)
                     /locus_tag="AC16FV_11935"
     CDS             complement(822..2309)
                     /locus_tag="AC16FV_11935"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HIR2850794.1"
                     /translation="MHSEIPKIKQEAGGLAEKAFDIHANAIFLVPAKDAALNTLPYGK
                     EIAQRLKRAGHKLDGDKVFTTELPNANATRVAIAAIDPAVSAFELLTLARQLVAAHKA
                     QKPQQIAVACFGLAPKTAEHACEAVLAALLAADFPMPDYKSKPDKPTRLREIHIYGYA
                     SADGYARTIAEAKGNNLARWLTALPPNELTPGHYRRRIEKLAREYKWKIEFLDIKKLK
                     ARGAGAFLAVAQGSPEPDAGILHLRYTPKKPSSKTALALVGKGICFDTGGTNLKPAKY
                     MHGMHEDMEGSAVALGTLLALTELKADFPIDCWLALAQNHIGPKAYKQNDVVKAANGK
                     TIEIVHTDAEGRMVLADTLYFASQQKPKLIVDFATLTGACVGALSTRMSGTLTNREKL
                     VPILIEAGNASGERVWPFPLPKDYEEALKSDIADIKQCTLDSDADHILAATFLKKFLV
                     NDPDWVHVDLSAGNHKGGLAHIPTDTTGFGVRFTLNLLMDQKVIG"
     gene            2792..>4177
                     /gene="aceE"
                     /locus_tag="AC16FV_11940"
     CDS             2792..>4177
                     /gene="aceE"
                     /locus_tag="AC16FV_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159754.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR2850795.1"
                     /translation="MAAVTPIPPKTDVDAQETQEWLDALSAIIQQEGPERAHYLIEKQ
                     IELARAAGINVPYAANTAYVNSIPPDKEVRSPGNHALEDRIRSYVRWNAVAMVLKANK
                     GDSNLGGHIASYASAATLYDVGFNHFWHAPSDKHGGDLIYMQGHSSPGFYARAFMLGQ
                     LTAEQMDHFRREVDGTGLSSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLHAR
                     GIVNTEGRKVWCFLGDGEMDEPESLGAIGMAGRENLDNLIFVVNCNLQRLDGPVRGNS
                     KIIQELEAGFRGYGWNVIKLIWGSYWDPLLARDRMGLLRKRMEECVDGDYQTFKARDG
                     AYVREHFFSKYPELKELVASWSDDDIWRLNRGGHDPHKVYAAYHAAIHHKGQPTVILA
                     KTIKGYGMGEAGEAQNITHQQKKMGTTSLQAFRDRFTLPLTDEQLDKLEYIKPAKDSP
                     EYKYMRARRDAL"
BASE COUNT          883 a         1152 c         1196 g          946 t
ORIGIN      
        1 gccgatcttc ataaggttcg agattttgtc taattctggc cgatagattt tttcgagttc
       61 ttcatattgc ccttgggcca ccataaaata gtcgtgcctt atctcgataa gtcctagccc
      121 ttcgaaattg ctcatgtatg cgacgatgtt ttgtggaaat gaaagcttag tctttgtctc
      181 taagcccgta agaagttggc ctagcggtat ccactccttt acgccattct ttatgccctt
      241 gattatgcgg gcttccaaga aaggaatatc gcctctaatc tgttgaagaa gcagagcttc
      301 atcaggtgaa agattattga taacgttaac gaagctaggg tgtgctgtat gggtagactc
      361 gaatacagag gctttagcaa gcagattaac atatagatca cttaactgat catcagttac
      421 ataagtcagt ttttctgtga ttggaacgcc aacttctggg gacacttcaa caattttttc
      481 tggagatgcg gtacttaatt gttcacgata tttttctagg tttttttcaa gcgctatctt
      541 ggatcgttca ttcaataaag ccaaaggcca taaaagtgta ttgcctagac ccagaacggc
      601 gcctaaagct ttgccgactt gagttactcc aggccttgca agatcaccat atatttgttt
      661 caaaagccca ggaacctgag caacctcttt taatatggga agaatttcat ttggcatgtt
      721 agtcccattc ttcatataag ttatttaaac ggtgcgcgga gcgcacccta cattaaataa
      781 tctgaacccc agcttttgcc ggagtgacga cagaaaaaaa attaaccgat caccttctga
      841 tccatcaaca aattcaaggt aaacctgacc ccaaaccccg tcgtgtcggt tggaatatgc
      901 gccaatccgc ccttgtgatt ccccgccgac aaatccacgt gcacccaatc cgggtcgttc
      961 accaggaact ttttcaggaa cgtcgctgca aggatgtggt cggcgtcgct gtcgagcgtg
     1021 cattgcttga tgtcggcgat atcgctcttg agtgcttctt cgtaatcctt gggcagcggg
     1081 aacggccaga cgcgctcgcc ggaggcgttg ccggcttcaa ttaaaatggg aaccagcttt
     1141 tcgcggttgg tgagcgtgcc gctcatgcgc gtgctcagcg cgcccacgca ggcaccggtg
     1201 agggtagcga aatctacaat tagcttcggt ttctgctgcg aggcgaaata aagcgtgtcg
     1261 gccagaacca tgcggccttc ggcgtcggtg tggacgattt caatcgtttt gccattggcc
     1321 gccttcacca cgtcattctg tttgtaggcc ttgggcccga tgtggttctg cgccagcgcg
     1381 agccagcagt ctatgggaaa atctgctttc agttcggtga gcgcgagcag cgtgccgagg
     1441 gccacggcgc tgccttccat gtcctcgtgc atgccgtgca tgtacttggc cggtttcaga
     1501 ttggtgccac cggtgtcgaa acagatgccc ttgccgacca gcgcgagcgc ggttttggat
     1561 gatggttttt tcggcgtgta acgcaggtgc aggatgccgg catccggttc cgggctgccc
     1621 tgggccacgg cgaggaaggc cccggcaccg cgcgccttga gttttttaat atcaagaaat
     1681 tcgattttcc atttgtactc gcgtgccagc ttctcgatgc gccggcggta atggcccggt
     1741 gtgagttcgt tgggcggcag cgcggtgagc cagcgcgcga ggttgttgcc tttggcctcc
     1801 gcgatagtgc gggcgtagcc gtcagccgac gcgtaaccat atatatgtat ctcacgcagc
     1861 cgggtcggct tgtccggctt gctcttgtaa tccggcatcg ggaaatcggc ggccagcagg
     1921 gccgccagca ccgcctcgca ggcgtgttcg gctgtttttg gcgcgagtcc gaaacaggcc
     1981 acggcgattt gttgcggttt ctgggctttg tgtgcggcga cgagctggcg cgccagtgtc
     2041 agcagctcga aggcgctgac ggccggatcg atcgcggcga tggcgacccg ggtggcgttt
     2101 gcattgggca gctcggttgt gaagaccttg tcgccgtcga gtttgtgtcc ggcacgtttg
     2161 aggcgttggg cgatttcctt tccgtaaggc agcgtattca gcgcggcatc cttggccggc
     2221 accagaaaaa tggcatttgc atggatatcg aacgcttttt ccgctaaacc accggcttct
     2281 tgcttgatct tcggaatctc ggaatgcatg gatgcctcaa gttaaaattt atatcgaaat
     2341 ggggcctaaa acgctgtttg tcttgacctg tttggacaaa aaaacgatca taccgcgcgc
     2401 aaaaaggaga tgatacggtt gtgccggtgg aatggtgaga ccggccgacg acgtatgacg
     2461 catggcacga acagaagtcc tgttttgccc gctgccgcca acccggccgt aagacacggc
     2521 cggacactgt ccccggcccg agtttgcccg tccggattat ccgcgggagg cagagttatt
     2581 aacccggcac gattgttatt aaacgaaggg catgcctgat ctaacagtac agcgttacga
     2641 tggataggcg tgccgggtta ataacaaaat tcattaagat ttctgattat tgcgacggca
     2701 tgatttatat ggatgtgata ccgagcgtcg aatatgcccg aatggctcaa atagtttcat
     2761 gccgtcgcaa taatctgagg agtgcgagac catggccgct gtgaccccga tacccccgaa
     2821 gaccgacgtc gatgcccaag agacccagga atggcttgat gcgctgtcgg ccatcatcca
     2881 gcaggaaggc cccgagcgcg cgcattacct gatcgagaag cagatcgagc tcgcgcgcgc
     2941 cgcgggcatc aacgtgccgt acgcggccaa taccgcctac gtcaactcga ttccccccga
     3001 caaggaagtt cgttcgcccg gcaaccacgc gctcgaggat cgcatccgtt cgtacgtgcg
     3061 ctggaacgcc gttgcgatgg tactgaaggc caacaagggc gattccaatc tcggcggaca
     3121 catcgcgagc tacgcctcgg ccgcgacgct ttatgatgtt ggcttcaatc acttctggca
     3181 cgccccatcc gacaagcatg gcggagacct gatctacatg cagggccact cgtcgccggg
     3241 tttctacgcg cgcgccttca tgctcgggca gctgaccgcc gagcagatgg atcatttccg
     3301 ccgcgaggtc gacggcacgg gtctgtcctc ctacccgcat ccctggctca tgccagattt
     3361 ctggcagttt ccgacggtgt ccatgggcct cgggccgatc caggcgatct accaggcgcg
     3421 cttcatgaaa tacctgcacg cgcgtggcat cgtcaacacc gaagggcgca aggtctggtg
     3481 cttcctcggc gacggcgaga tggacgagcc cgagtcgctc ggcgccatcg gcatggccgg
     3541 gcgcgagaat ctggataatc ttattttcgt ggtgaactgc aacctgcagc ggctcgacgg
     3601 tccggtccgc ggcaacagca agatcatcca ggaactcgag gccggcttcc gcggctacgg
     3661 ctggaacgtg atcaagctca tctggggcag ctactgggat ccgctgctgg cgcgcgaccg
     3721 catggggctg ctgcgcaaac gcatggaaga gtgcgttgac ggcgattacc agacgttcaa
     3781 ggcgcgcgat ggcgcctacg tccgcgagca cttcttcagc aagtacccgg agctgaagga
     3841 gctggtggcc agctggtcgg acgacgacat ctggcggctc aatcgcggcg gccatgaccc
     3901 gcacaaggtg tacgcggctt accacgccgc gatccatcac aagggccagc cgacggtgat
     3961 cctcgccaag accatcaagg gctacggcat gggcgaggcc ggcgaggcgc agaacatcac
     4021 gcaccagcag aaaaagatgg gcacgacctc gctgcaggcg ttccgcgacc gcttcacgct
     4081 gccgctgacg gacgagcagc tcgacaagct ggaatacatc aagccggcaa aagactcgcc
     4141 ggagtacaag tacatgcgcg cgcggcgcga tgcgctc
//
LOCUS       DBNGZE010000460         3348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_827562, whole genome shotgun sequence.
ACCESSION   DBNGZE010000460 DBNGZE010000000
VERSION     DBNGZE010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_827562"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="AC16FV_11945"
     CDS             complement(<1..566)
                     /locus_tag="AC16FV_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR2850796.1"
                     /translation="MNTQPLRKYLFPWRPGNRFELLVDGRRFFPRMLEAIERARHHVL
                     LEMYLFESGTVATRFIDAFVRAAARGVTVKLLLDDFGARGLLRQDREKLARGGVELAL
                     YNPLHFRKWHRNMFRDHRKLMIVDGEIAFVGGAGITDAFDAPGHPELSWRETAVSVHG
                     PAAADWQALFLRVWNRHVSSTLRLPEAAP"
     gene            complement(590..1645)
                     /locus_tag="AC16FV_11950"
     CDS             complement(590..1645)
                     /locus_tag="AC16FV_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850797.1"
                     /translation="MPQWVRAVFMAVCLWPALVLANPFSTLPLQPSGEIPATGKILVT
                     LEDGDYVLPHFSPDSRYLAFAHVVMQGKTELTEIQALDLKTLKVITLLDAKGSREFAV
                     YKGFVTGFVWKDATTLKAGISDGDVNGVDLIFDVAAGKLIEKKPFSLTDEASGKEEVL
                     TPELTAAFPSIPPPVLANALANGFKVGDKKYIVQKNYWKQDNHIWLLDTGSRQMTRLL
                     DIPDVWIYSLRGAFASGNAFVLLVAYGPEAWLARHLGGKLELLYRFPVKNYQQTALRV
                     ENVRGERVLFQISTGAEYEKRENFLFVYDRSGLRKIRETAPIHDLDVDNAGRLMGLSQ
                     WKGNHRRLVVREFKDSR"
     gene            complement(1731..2156)
                     /locus_tag="AC16FV_11955"
     CDS             complement(1731..2156)
                     /locus_tag="AC16FV_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR2850798.1"
                     /translation="MTDTEARNELEAQILAAQEGRITSDDLLKTLMGSQVFMPVQDEK
                     APVLNVQRSTRAQPLVLSAEEGTPILVLFSSPERAKPFLDNYPGFTGGILESFQWVLQ
                     KMGSGYGIMLNPDSELGFDMEPETVQGLIQQLAGGETQH"
     gene            complement(2310..2639)
                     /locus_tag="AC16FV_11960"
     CDS             complement(2310..2639)
                     /locus_tag="AC16FV_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR2850799.1"
                     /translation="MPQIQERLEVEFDEDGLLLNPAQWSEAVAQEIARHDGIGRLTDE
                     HWEVIRSLREHYAKFGVAPAMSHICRSHGKDRHWIHDLFHSCLSAWRVAGLPNPGEEA
                     KAYMSDM"
     gene            complement(2743..>3348)
                     /locus_tag="AC16FV_11965"
     CDS             complement(2743..>3348)
                     /locus_tag="AC16FV_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR2850800.1"
                     /translation="RLDDYLDDFFFDQSYENLIGAARNGKNGQVVNLLVGRKIADVDL
                     PGLPHLFSGITWDYRGTPVLATPNLKDGAITIIDMKKWKTIKHIKTLGPGFFLRSHEN
                     TPYAWVDVSFSPSKDTIQILDKDKLKIVKAFKPEPGKTAGHVEFTRDGRYALVSIWEM
                     DGALVVYDAATFKEVKRLPMKKPLGKYNVYNKITRSAGTSH"
BASE COUNT          635 a         1013 c         1001 g          699 t
ORIGIN      
        1 ggcgcggctt ccggcaggcg aagggtgctg gaaacgtggc gattccacac gcgcagaaac
       61 agcgcctgcc aatccgccgc cgcggggccg tgcacgctca cggccgtctc gcgccagctg
      121 agttcgggat gtcccggcgc atcgaaagcg tcggtgatgc ccgcgccgcc gacaaaggca
      181 atctcgccat ccacgatcat cagcttgcgg tgatcgcgga acatgttgcg gtgccatttg
      241 cgaaagtgga gcgggttgta gagcgccagt tcgacgccgc cccgggcgag tttctcccgg
      301 tcctgccgta aaagtccgcg cgcgccgaaa tcatcgagca gaagcttgac tgtcacgccg
      361 cgcgcggcgg cgcgcacgaa ggcatcgatg aagcgcgtgg cgactgtgcc agactcgaaa
      421 agatacattt ccagcagcac gtgatggcgc gccctttcga tagcctccag catgcgcggg
      481 aagaaacgcc ggccgtccac cagcagctcg aaacggttgc cgggacgcca gggaaacaga
      541 tatttacgca atggttgcgt gttcatggag agtcgcggca gcgacttgct cagcgggagt
      601 ccttgaattc cctgacgacc agccggcgat gatttccctt ccactgcgac aggcccatga
      661 gtcttcccgc gttatccacg tcgagatcgt ggatgggcgc ggtttccctg atttttctca
      721 accccgacct gtcgtacaca aacaggaagt tttccctttt ttcgtattcc gctccggtgc
      781 tgatctggaa caacacgcgc tcgccgcgca cgttttccac gcgcaatgcg gtctgctgat
      841 agttcttcac gggaaagcgg taaagcagct ccagcttgcc gccaagatgt cgcgccagcc
      901 acgcctctgg gccgtaggcc acgagcagga caaaggcatt gcccgaggca aaagcgccgc
      961 gcaggctgta aatccagacg tccgggatat ccaggagcct ggtcatctgc cggctgccgg
     1021 tgtcgagcaa ccagatatga ttgtcctgtt tccagtaatt tttttgcacg atgtattttt
     1081 tgtcgccaac cttgaagccg ttggccagcg cgttcgccag caccggcggc ggaatggagg
     1141 gaaatgcggc ggtcagttcg ggggtcaaca cctcctcttt gcctgacgcc tcgtcggtca
     1201 gcgaaaacgg tttcttttcg atcagcttgc cggcggccac gtcgaaaatc agatcgacgc
     1261 cgttcacgtc gccgtccgaa atcccggcct tgagcgtggt ggcgtccttc cagacgaacc
     1321 cggtgacgaa acccttgtac accgcgaatt cgcgcgaacc cttggcgtcg agcagggtga
     1381 tgaccttgag cgttttgaga tcgagcgcct gaatttcggt caactcggtt ttcccttgca
     1441 taacgacgtg ggcgaacgcg aggtagcgcg agtcgggcga gaagtgcggc aatacataat
     1501 cgccgtcctc cagcgttacg agaatttttc ccgtggctgg gatttcaccc gaaggttgca
     1561 gcggcaatgt ggaaaacgga ttcgccagca ccagcgctgg ccacagacac actgccatga
     1621 acaccgcacg cacccactgt ggcatgttcg tttcgcccca tcccccggat cgacgccgcc
     1681 aaagaatata cctcccgcga ccggaagggg agggttcggc cgatgccggg tcagtgctgg
     1741 gtttcgccgc cggcgagctg ctggatcagg ccctgcaccg tctcgggctc catgtcgaaa
     1801 ccaagctcgg aatcaggatt gagcatgatg ccgtagccgc tgcccatctt ctgcagcacc
     1861 cactggaacg attcgagaat gccaccggtg aagccgggat agttgtcgag aaaaggcttg
     1921 gcgcgctcgg ggctggagaa caacaccagg atgggcgtgc cctcctcggc cgacagcacc
     1981 agtggctggg cgcgcgtgga gcgctgcacg ttgagcaccg gcgccttttc gtcctgcacc
     2041 ggcatgaaca cctgcgagcc catgagcgtc ttgagcagat cgtcgctcgt gatgcggcct
     2101 tcctgcgcgg cgaggatttg cgcttccagc tcgttgcggg cttcggtgtc ggtcatggcg
     2161 tgtctcaaaa aaagagccgc catgctcggg tatcccaccg ccgattgcaa ccccgtgaat
     2221 gttacggaat tgcgtcgtgt ccacgtcgga gcgcgatcat tcggtttgcc tgatccgggt
     2281 caacccccaa ggtcagcccg atggccgggc tacatgtcgc tcatataggc cttggcttcc
     2341 tcgcccgggt tgggcaaacc cgccacgcgc cacgcgctga gacaactgtg gaacaggtca
     2401 tggatccagt gcctgtcctt gccatgactg cggcagatgt ggctcatggc gggggccaca
     2461 ccgaacttcg cataatgctc ccgcagcgaa cgaatcactt cccagtgttc gtccgtgagc
     2521 cggccgatgc catcgtgccg cgcgatttcc tgcgcgacgg cttcgctcca ttgcgcggga
     2581 ttcagcagca gaccgtcctc gtcgaactcc acttccagcc gttcctggat ttggggcatg
     2641 tacgcctcct tcccggtttt ccggagggtg agttttattc aagatttatt atgcgcccaa
     2701 tccgggagag gtgtacggat agtgatacaa ccggtgtggg attcagtggc tggtgcccgc
     2761 ggaacgggtg attttgttgt agacgttgta cttgcccaag ggttttttca tcggcaagcg
     2821 cttcacttcc ttgaaggtcg cggcgtcgta caccaccagc gcgccgtcca tttcccagat
     2881 acttaccagc gcgtatctgc cgtcgcgcgt gaattcgacg tgcccggcgg ttttgcccgg
     2941 ttcgggcttg aacgctttga cgatcttcag cttgtccttg tcgaggatct ggatcgtatc
     3001 cttgctcgga ctgaaggaaa cgtcgaccca ggcataaggc gtgttctcat gcgagcgcag
     3061 gaagaacccg gggcccaggg tcttgatatg cttgatcgtc ttccattttt tcatgtcgat
     3121 gatggtgatc gcgccatcct tcagattcgg cgttgccagc accggcgtgc cacggtaatc
     3181 ccaagtgatg cccgaaaaca gatgcggtaa cccgggcagg tccacgtcgg cgatcttgcg
     3241 cccgacgagc agattcacca cctggccgtt cttgccgttg cgcgcggcgc cgatgagatt
     3301 ttcataagac tgatcgaaga aaaagtcatc gagatagtca tcgagccg
//
LOCUS       DBNGZE010000461         6545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_967174, whole genome shotgun sequence.
ACCESSION   DBNGZE010000461 DBNGZE010000000
VERSION     DBNGZE010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_967174"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..3047)
                     /locus_tag="AC16FV_11970"
     CDS             complement(300..3047)
                     /locus_tag="AC16FV_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR2850801.1"
                     /translation="MKYGTDISRFRYLIAVLLVCVLTTVNSGHASAAERLRIVAAQTA
                     ADTGLIGSLADAFRARYPDVSIDIQPAGALATLDLARRGLADLVITHAPEGEKLFIEE
                     GYGLLRTTIMYNEFAIMGPPDDPLKLSRERDLATVLQHMAREQVSFLVPGKRSGTLNK
                     LNELWLMAGIEPNWVGYEISNASSAATLRDAALFGHYTFSDLGTYLVNRDLIAGGVIP
                     LYRDDALLRNYYSAIVVNQERHPQANQKLAERFLDFLVSDEAQDRIRRFGEERFGTIL
                     FTPAASLDEGLKARRDRVELERKNQELKLMSGLVLVFVALTVLASLLLVRMRKLEKIR
                     RRSEERFELAVAGTNDGIWDWDVLADKAYLSPRLKQMLSIDEADNFFTNPKQVIADRV
                     VQNDQLSLLGKLESYLQKGNGDDLFQAEFRMAAGNDTRWLMMRGKAIRGPMGQAVRVS
                     GSLTDITEHKAQEATIEHQALHDMLTGLPNRALLRDRLQHAIRVATRQNTYLSLLVMD
                     LDRFKDINDTLGHHVGDLVLQQVGARLQQILRPSDTVARLGGDEFAVLLPVTDATYAN
                     HVAQKIILALKKVFELGRHSLYVGGSLGVAVFPEHGQDAETLIQHADVAMYLAKRSNS
                     GCAFYDPRQDRYSVTRLALEKDLHDAIDNNALELYYQPLLCLRTKRVTGAEALLRWKH
                     PQRGMIPPDEMIPIAEQTGLIKPLTMWVLNTAMHQCEAWRRQGLHLKVAVNLSVWNLQ
                     DPTLVDALKAIFATWNIPPSCLELEITESAMMADPDHALEVLTELNNMGINLAVDDFG
                     TGFSSLAYLKKLPVHSLKIDKSFVIGMAREENDIMIVRSTVELAHNLGLGVVAEGVES
                     QEILDLLARHGCDVAQGYHLGRPMPVTDVTRYLEEHRGDLSNDDSCPDNNHLRPGPAL
                     S"
     gene            complement(3176..4273)
                     /locus_tag="AC16FV_11975"
     CDS             complement(3176..4273)
                     /locus_tag="AC16FV_11975"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate
                     methylesterase/protein-glutamine glutaminase"
                     /protein_id="HIR2850802.1"
                     /translation="MHEERKIKALIIDDSAVMRQVLTTVLNQDPEIHVVGAAANPLIA
                     REKIKTLKPDVLTLDIEMPGMDGLTFLEKLMRLHPLPVVMVSSHTEKGAMATLRALDL
                     GAVDFVAKPQGGVREGLSMDLMEEIRAKVRAAAGAHVRPAPPAPAAKLRVPQSSVASL
                     GCRDGIIVIGASAGGTQAISEIMAKLPRSTPGIAIVQHMPPKFTASFAERLHQLSNID
                     VREAKSGDRLKSGLALIAPGGYHMAIMRAGGGYAVRVYEDEPVNRHRPSVDVLFDSAA
                     RIAGADALGIILTGMGSDGARGLHAMKMAGAHTVAQDEKSCVVFGMPEQAIRLGAVSS
                     VVSLERIADHITAWAKPVDSREEGQRLKESP"
     gene            complement(4311..4916)
                     /locus_tag="AC16FV_11980"
     CDS             complement(4311..4916)
                     /locus_tag="AC16FV_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HIR2850803.1"
                     /translation="MKSIQPRRTNGTRLPSNIDADLTRMTIHIGGLHASRKPLLLDTV
                     LGSCIAACLYDPVLGMGGMNHFMLPEGSDPGDPTSTRYGVNAMELLISDLMKLGADRR
                     RFQAKVFGGGHVLNIRESLDGVPQRNIDFVRRFLEAEQIPVVREDLGGFQPRRVLFQT
                     HTGKVFLKYLGNNEAERTAQEEVVYLISLKKQKLDGEVTLF"
     gene            complement(4918..5817)
                     /locus_tag="AC16FV_11985"
     CDS             complement(4918..5817)
                     /locus_tag="AC16FV_11985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR2850804.1"
                     /translation="MKQMVSRHTVTGKISSILDDSRLTISDAEFQQLREFIHAHTGIA
                     LSEHKKALVCSRLSKRLRHHDLQHYSDYYVLLVENDPEGMELMAMINCITTNKTDFFR
                     EPHHFHFLSEHVFPAYKQNPNRERPLRMWSAASSTGEEAYSLAMTALEAMPSFNEQDI
                     RILATDIDTDVLSRAASGVYTEVQASHIPEALLHRYFLKGQGAHQGEVMAKPLLKALV
                     HFHWLNLQEDPWPMQAKFDVIFCRNVLIYFDKPTQQKLFQRMAGALKKDGYLMLGHSE
                     AMHGLNDLFKPVGHSIYQCRGNK"
     gene            complement(5910..>6545)
                     /locus_tag="AC16FV_11990"
     CDS             complement(5910..>6545)
                     /locus_tag="AC16FV_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR2850805.1"
                     /translation="QTNLLALNAAVEAARAGEQGRGFAVVASEVRNLAQRSASAAKEI
                     KTLIQDSVEKVQDGTQLVGTSSKTLEEIVESVKNTAEIIAEISASSQEQASGIEQVNR
                     AIMQMDGVTQTNAAQVEELSGTSQSLASQAEQLRALVSHFTFRPEAMKRFGAGATEKT
                     VSASSTRPEASASAVETGVLENAGKSRNVQYLKPRVVSQKASATGGDWTEF"
BASE COUNT         1296 a         1946 c         1920 g         1383 t
ORIGIN      
        1 aggcaatcaa gagaccaaac ttcccctccc cagcacattt gcactaacgg cgctccttaa
       61 agtaatactt ttaaagtaat gccttaggaa tttcccccgc tgtattcggt caccaggggt
      121 tggagaatgt aattccgcac cgtccgctgg cacgattctt ttatcctcta caggaggtaa
      181 gtaccgtaat tgccggaata taatatgtat tcgtcatggg cgccgcaatg gatcacaaaa
      241 aggcgcatgt atcatacctt ttggcgggtt ataacgggta acgacgacgg cgtgagccgt
      301 caggacaagg cggggcccgg acgcaagtga ttattatccg ggcagctgtc gtcattggac
      361 agatcgcccc gatgctcttc cagatagcgc gtgacatcgg tcaccggcat gggacgcccc
      421 agatgataac cctgggcaac gtcgcaccca tggcgcgcca gcaaatccag aatctcctga
      481 ctttccacgc cctccgcgac cacgcccagg ccgagattgt gggccagctc gactgtcgag
      541 cggacaatca tgatgtcgtt ctcctccctg gccattccga tgacgaacga cttgtcgatc
      601 ttgagcgaat ggaccgggag tttcttaaga taagccagtg aagaaaagcc ggtgccgaag
      661 tcatccaccg ccaagttgat gcccatgttg ttgagctccg tcagaacctc gagcgcgtgg
      721 tccggatcgg ccatcatggc gctttcggtg atttcgagct cgagacacga aggcggaata
      781 ttccaggtgg cgaagatggc cttgagcgca tccaccagtg tcgggtcctg aaggttccag
      841 acagacaggt tgacggcaac cttcaggtga aggccctgcc ggcgccaggc ctcgcactga
      901 tgcatcgcgg tgttcagtac ccacatggtc agcggcttga tcaggccggt ttgttcggcg
      961 atcggaatca tttcgtcggg cgggatcatc ccgcgctgcg gatgtttcca gcgcagcaac
     1021 gcctccgccc ctgttacgcg cttggtgcgc agacagagca gcggctggta atacaattcc
     1081 agcgcgttgt tgtcgatggc gtcatgcaga tctttttcca gcgccaggcg cgtgacgctg
     1141 tagcggtcct gacgcggatc gtaaaacgcg cagccgctgt tggaacgctt ggcgagatac
     1201 atcgccacgt ccgcgtgctg gatcagcgtt tcagcgtcct ggccgtgctc cgggaacacg
     1261 gcgacgccga gacttccgcc cacgtaaaga ctgtggcgac cgagctcgaa taccttcttc
     1321 aacgctagaa tgattttctg cgccacgtga ttggcatagg tggcatccgt gaccggaagc
     1381 aggacggcga attcatcacc gcccaggcgc gccaccgtgt ccgaggggcg cagaatctgt
     1441 tgcagccttg ctcccacctg ctgaagcacc agatcgccga catgatgacc gagcgtgtca
     1501 ttgatgtcct tgaagcggtc aaggtccatg accagaagcg agagataggt gttctgtcgc
     1561 gtggccaccc gaatggcatg ctgcaggcgg tcgcgcagca acgcgcggtt ggggagaccg
     1621 gtcagcatgt cgtgcaacgc ctgatgctcg atcgtcgctt cctgcgcctt gtgctcggtg
     1681 atgtcagtga gcgagcccga aacgcgcacg gcctgaccca tgggaccgcg tatggccttg
     1741 ccgcgcatca tgagccagcg ggtgtcgttg ccggccgcca tgcgaaactc ggcttggaac
     1801 aggtcgtcac cgttgccttt ctgcaaatag gattcgagct tgcccagcaa ggaaagctga
     1861 tcgttctgaa ctaccctgtc ggcaatgact tgcttggggt tggtgaaaaa attatccgct
     1921 tcatcgatag acagcatttg tttcaggcgc ggtgataaat aggccttgtc ggcaagcacg
     1981 tcccagtccc agataccgtc attggtgccg gccaccgcta actcaaatcg ttcctcgctg
     2041 cgccgccgga ttttctcaag cttgcgcatg cgtaccagca gcaggctggc gagcacggtc
     2101 agcgccacga acaccagcac cagccccgac atcagcttca gttcctggtt ctttcgctcc
     2161 agctccacgc gatcccgacg cgccttcagg ccttcgtcaa ggctggccgc gggcgtgaac
     2221 agaatcgtcc cgaatctttc ctcaccgaaa cgacggatgc gatcctgggc ctcgtcggaa
     2281 accagaaagt ccaggaaacg ctcagccagt ttctgattgg cctgcgggtg acgttcctga
     2341 ttgacgacaa tggctgagta gtaattacgc agcagggcgt catcgcgata caggggaatc
     2401 acgccgcccg cgatgaggtc acggttgacc aggtaggttc caagatcgct gaaggtgtag
     2461 tgtccgaaca gcgcggcatc acgcaatgtc gccgccgaac tggcgttact gatttcgtat
     2521 ccgacccagt tgggctcgat acccgccatg agccacagct cgttcagttt gtttaatgtc
     2581 cccgagcgct tgccgggaac aaggaaggat acctgctcgc gggccatgtg ttgcaaaacc
     2641 gtggccagat ccctctcacg ggacagtttc agcggatcat ccggcggccc catgatcgcg
     2701 aattcgttgt acatgatcgt ggtccgcagc aggccataac cctcttcgat aaacagcttc
     2761 tcgccttccg gggcgtgggt aatgaccaga tcagccagtc cgcggcgtgc cagatcgagc
     2821 gtggcaagcg cgcccgcggg ctggatatcg atgctgacat ccgggtaccg cgcgcggaag
     2881 gcatccgcca gcgaaccgat caatccggta tccgcggcgg tttgcgccgc cacgatgcgc
     2941 aggcgttcgg cggccgatgc atgtccggaa ttgaccgtcg tcagcacgca gaccaacagg
     3001 acagcgatca gataccgaaa acgggaaatg tcagttccgt atttcatgtc gtccttcacg
     3061 ccagcggcag gaaacggcca ttcaggctgc gtgaagttat cactcccttt atccctttat
     3121 aaaaatagac cattttcgat ccagtttccg atgaaatcct tttcaaatag cgggcttaag
     3181 gcgattcttt cagtcgctgg ccttcttcac gtgaatcaac cggtttcgcc caagccgtga
     3241 tgtggtcagc gatgcgttcg agtgacacca cgctggatac tgccccgagg cgaattgcct
     3301 gctcgggcat gccaaatacc acgcagcttt tttcatcctg ggcgacggta tgcgcgccgg
     3361 ccattttcat ggcgtgcaag ccgcgcgcgc cgtcactgcc catgcccgtc aaaatgatgc
     3421 ccagggcatc ggcgccggca atgcgggcgg cggaatcgaa cagcacatcg acggaaggac
     3481 ggtgccggtt caccggctca tcctcataaa cccggacagc atatcctcca ccggcgcgca
     3541 tgattgccat gtgatagccg cccggggcga tcagcgccaa gcccgacttc agccggtcac
     3601 cgcttttggc ctcgcgaacg tcgatgttgg acagttgatg caggcgttcg gcaaacgagg
     3661 ccgtaaactt tggcggcatg tgttgcacga tggcgatgcc tggtgtcgag cgcggcagtt
     3721 tcgccatgat ttccgaaatg gcctgtgtgc cgccggcgga ggcgccgatg acgatgatgc
     3781 catcgcgaca gcccaacgac gcgacagaag attgcggaac acggagtttg gcggctggag
     3841 ccggcggcgc ggggcgcaca tgcgcgccag ccgccgcgcg taccttggcg cggatttctt
     3901 ccatcaagtc catgctcagg ccctcgcgca caccgccctg cggcttggca acgaaatcca
     3961 cggccccgag atcgagggcg cgcagcgtgg ccatggcgcc tttttccgtg tgcgaggaca
     4021 ccatgaccac cggcagcggg tgcaagcgca tcagcttttc caggaaggtc aggccgtcca
     4081 tgccgggcat ctcgatatcc agcgtcagca cgtcgggttt gagtgtcttg attttctcgc
     4141 gcgcgataag cggattggcc gccgcgccga cgacgtggat ctccggatcc tgattcagga
     4201 cggtcgtcaa cacctgacgc atcaccgccg agtcgtcgat gatgagggcc tttatttttc
     4261 gttcctcgtg catgctggta tccgacggct tcaggggtat acgttgtttg tcaaaaaagc
     4321 gtaacttcgc catcgagctt ctgcttcttc aggctgatca ggtaaaccac ttcttcctga
     4381 gcggtgcgtt cggcctcgtt gttgcccaaa tacttgagaa aaaccttgcc ggtgtgcgtc
     4441 tgaaacagca cccgccgcgg ctgaaagccg cccagatcct cgcgcacgac gggaatctgc
     4501 tccgcttcca ggaaccgtct tacgaaatcg atgttgcgct gcggcacgcc atcgaggctt
     4561 tcgcggatat tgagcacgtg gccaccaccg aaaaccttgg cctggaaacg ccgacgatcg
     4621 gcgccgagtt tcatgagatc gctgatgagc aattccatgg cgttgacgcc gtaacgcgtg
     4681 ctggtggggt ctccgggatc ggagccctcc ggcagcatga agtgattcat gcctcccatg
     4741 cccaaaaccg ggtcgtacag acaggcggcg atgcacgaac ccagcaccgt gtccagcaac
     4801 aacggcttgc ggctggcatg caggccacca atatggatcg tcatgcgcgt cagatcggcg
     4861 tcgatattcg acggcagtcg tgttccgttg gtgcgtctgg gttggatgga tttcatgtta
     4921 tttattgccc cgacactgat aaatgctgtg accgacgggc ttgaagagat cgttcaagcc
     4981 atgcatggcc tccgaatggc cgagcatcag atagccgtcc ttcttgagcg cgccggccat
     5041 gcgctgaaac aatttttgct gcgtgggctt gtcaaaataa ataagcacat tgcggcaaaa
     5101 aatgacgtca aactttgcct gcatgggcca gggatcttcc tgcaaattga gccaatgaaa
     5161 atgcaccagc gctttgagga ggggcttggc catcacttct ccctgatgtg cgccttgccc
     5221 tttcaggaaa tagcgatgca atagcgcctc gggaatgtgc gacgcctgga cctctgtgta
     5281 gacgccgctg gcggcgcgtg acaatacatc ggtatcgata tcggtcgcga ggatacggat
     5341 atcctgctcg ttgaaggacg gcatggcctc cagcgcggtc atggcaagcg aataggcctc
     5401 ttccccggtc gaggaggcgg cgctccacat ccgcaacggc cgctcgcgat tgggattctg
     5461 cttgtaggcc gggaacacgt gttcggacag aaagtgaaaa tgatgcggct cgcggaagaa
     5521 atcggtcttg ttggtggtga tgcaattgat catggccatc aattccattc cttccgggtc
     5581 gttctcgacc agcagcacat aatagtcgga atagtgttgc aggtcgtgat ggcgcagccg
     5641 tttggacagg cgcgagcaga ccagcgcctt tttgtgttcg gacagcgcaa tgccggtgtg
     5701 cgcgtgaatg aattcgcgca actgctggaa ttccgcgtca gaaatagtca gccggctgtc
     5761 gtccagtatc gagctgatct ttccggtaac ggtatggcgg gacaccattt gcttcatgcc
     5821 gcttcaatag tccttagccg ctgttgatgt tatcccggtg cgcgtcgcga gcttccgtcg
     5881 cgcccggggc tgttgaagaa cgggcctgat cagaattcgg tccagtcgcc ccccgtggca
     5941 ctggccttct gcgacacgac gcgcggcttc aggtactgaa cattccggga ttttcctgca
     6001 ttctccagca caccagtttc aacggcggaa gccgaagctt ccggccgcgt ggacgacgcg
     6061 gacaccgttt tttcggtggc gccagcgcca aaacgcttca tcgcctcggg tctgaaggtg
     6121 aaatgggata ccagcgccct caactgctcc gcctgcgagg ccagcgactg gctggtcccg
     6181 gacagctcct cgacctgcgc cgcgttcgtc tgggtgacgc cgtccatctg catgatggcg
     6241 cggttgacct gctcgatgcc ggaggcctgt tcctggctgg aggcgctgat ctcggcgatg
     6301 atctcggcgg tgttcttgac gctctccacg atctcctcga gtgtcttgct cgaggtcccg
     6361 acgagctgcg tgccgtcctg caccttctcc acgctgtcct ggatcaatgt cttgatctcc
     6421 ttggcagcgg aggcgctgcg ctgcgccagg ttacgcacct cggacgccac cacggcaaaa
     6481 ccgcgcccct gctcgccggc gcgcgcggct tcgaccgccg cattcagtgc cagcaggttg
     6541 gtctg
//
LOCUS       DBNGZE010000462        10697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_758835, whole genome shotgun sequence.
ACCESSION   DBNGZE010000462 DBNGZE010000000
VERSION     DBNGZE010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_758835"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="AC16FV_11995"
     CDS             <1..185
                     /locus_tag="AC16FV_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850806.1"
                     /translation="RETPALTAHLDSLAIAGGDILLLHEDYAHTVESLPRILQSLKDR
                     SLKFSRISDLGRHSET"
     gene            193..1797
                     /locus_tag="AC16FV_12000"
     CDS             193..1797
                     /locus_tag="AC16FV_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850807.1"
                     /translation="MYRSLLFVAALLGLVCTLPALSQDVTTTGTTYYISPAGKNWNNG
                     KSASQPFKTFAHAFDKMSAGDELVLLDGAYSEPLGTGYISYTDPSRGIGGAHSGQIPA
                     GVDADRVTRVRALNPGKVTVIGGLVIGRSFRKDSYIKIQGVTFEGGGSLYNTSHVTIK
                     DCGFHGPFSIGTNDHKEGNDFNLIEDVWIWASGERIIAINYRSQFNVWRRVLVRGDGC
                     NKPECRGDSNPNVGITVYDSSNVSLQNVMVVDRILADKDEPYGDFAVAQHTRGAHFFG
                     QSEWLGTISLRSPDTGYYMEPDHDGTIDPTIRISNAIAWDAAQLGFNLARAGTNNELE
                     NLTVRARGGDGVRIAPSLTSGTLRNVISVDALRKGINSRYPFSHVNVYNARQGAYQQN
                     SCDAGCYSSDPRADGRIPSLKYLVRIESGSKLKGSGYEGADIGANVLYRYGVDGSRYG
                     DLGFNALTSAPLWPWPNEERIKTEMCEKAGVTRGFCRARSLTHYIWEYLGNPMPADPY
                     APSAMPLKTQQSSNNARGGLIRRWFQ"
     gene            complement(1791..2816)
                     /locus_tag="AC16FV_12005"
     CDS             complement(1791..2816)
                     /locus_tag="AC16FV_12005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2850808.1"
                     /translation="MRKKEYLARGMAITGLHGLSSFLRGLRAGEIPILAYHRVFDIGQ
                     EQAFSFDPDLVSASSPAFAWQMLYLKKRYHPITFQTLLNAMDGKEQLPSRPVIVSFDD
                     GYDDNYLNAFPVLRSLGMPATIFVSTGYVGSGKPFWFDLATHILYHLPNGNLTVKDLG
                     ITLPLDTDVNSRRIAAGRLVGALKRVGNAQRLGILDWLEREHGDAMRPADFRLSRPLS
                     WDQIREMSAAGIEFGSHTVNHPILSRLDDEELRRELTDSRLRLEQELGKPAPVLAYPV
                     GGPEEFNDRVVRAATAAGYRLGVSYMPGVNRLGDMDRFRLRRQHVERYVSNAYFAGLL
                     SLPEIFH"
     gene            complement(2859..4112)
                     /locus_tag="AC16FV_12010"
     CDS             complement(2859..4112)
                     /locus_tag="AC16FV_12010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR2850809.1"
                     /translation="MPIIFIYLTALFIAPQLWVEPFVGLRVDFFVYPLWLLAAFLARE
                     KNPLTFTSLDKFFLLMLLWIILSMAVNGFQDNSMDIIVNYTKWFMLFKLVSMTVTSAG
                     RLRAVALMLVFFGLVLAMEGIEHKTSESGLGWAGQALGWIDPEAAAAGEPGRTRWINI
                     FDGPGVFCVIYTIALPFVMQYFVAPFSAGTRLLGAGLLGMLLVAIYFTGSRGGFLTTL
                     TIFALFVAVRYKVSLIKIAFVGGVISAAFMLAPSYITTLDDPSKSTKHRIDMWIEGVE
                     MVQYNPLFGIGKGNFRNYTGKLIAHNSSIEIMGETGLPGLFFWIGLLYMALKNVVYYV
                     RECENERDRAYATALGLSVVGYIVSSMFVTLEYETLYFLLGLCAALGFRLRQPNLFTE
                     RDFWVIAMTTVGWAILVKVFVMLYV"
     gene            complement(4139..5614)
                     /locus_tag="AC16FV_12015"
     CDS             complement(4139..5614)
                     /locus_tag="AC16FV_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR2850810.1"
                     /translation="MASENASLGRFFKHSTIYAFGNVLNRIAALLLLPVYTNYLTVTE
                     YGALELFYVVAAVVTGFLSVGIAHATLRFYFEHDAETDRHSVVTTSLTVSLLITSIGV
                     LLVGQWHEALARYVFGGPEYSRGILIILATLVLELSSQVSLAYLRAREYSMLFVGIMF
                     FKLVIQLAANTYFVVFRNMGVEGVLAGNFLAVAFGWGCLTIFVLKKCGYRFEWSKAVP
                     ALKYSFPFLLSTITALVSTYTDRFLVNSLLSLQILGIYALALKFSELLESLIGEPFNR
                     SYGAFRFSIMKEGNAAEIQARIVRYLLIGLCAVGLGIVYFANDLLRAMSDAAYWPAAG
                     ILPVLMVAGLLKVLNYPFQTGILYEKKTRHIFHIGLIAAVTSVTSNFLMIKWLGLVGA
                     CLAQVLTAAVVLVVTNRISQAYFRVSYDYRRLAMIAAVTGIFYLLSILLEGQQIFVAF
                     FAKILLYVTFIVALFFSGALHATETAWLRSALVRLIQRRDI"
     gene            complement(5660..6502)
                     /locus_tag="AC16FV_12020"
     CDS             complement(5660..6502)
                     /locus_tag="AC16FV_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HIR2850811.1"
                     /translation="MKNAFTIDVEDYYQVEAFAGVVNKNTWGSYESRVADNTRRLLDL
                     LDEYSVKGTFFVLGWVAQHQRALIREIADRGHEIASHGMSHTLVYTQTPDVFRDETRR
                     SKALLEDICQRPVIGYRAATYSITDKSLWALDILHEEGFRYDSSIFPMRHDRYGIPDA
                     QPYPNRLRTPAGYTLTEFPISVFRSGAFKLPVAGGGYFRLFPYPLTRWGLAQINRSGH
                     EFVFYLHPWEVDPGQPRIREAGAMSRLRHYLNLARTRPRLDRLLKDFSFTTMEDVLRS
                     QALL"
     gene            complement(6603..7817)
                     /locus_tag="AC16FV_12025"
     CDS             complement(6603..7817)
                     /locus_tag="AC16FV_12025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2850812.1"
                     /translation="MGKDITLSVVDSSAGLMNLRDEWNHLLDASSARSICLRWEWIHT
                     WWDIYHDCGVKPCVLLVRDDARLVGLAPFYLQIEKKPVLGSIRTLRFLGTGEPEQEEV
                     ASEYLDIVALTGYEDQVAKAVWAHLRDQEFWDQLVFNDVLEGSLVLTTLRHVLTAERI
                     PVALEKVGIRYSVALPPTWQEYIAMLDDGAAKRLPYKRRKFERAGRVEYKSVTEAEEL
                     DQAFDELIRLHTKRWKARGREGVFASSRFADYHRRLAKILLPQGMLNLGFLSLDGVNI
                     AAHYNLRHAGTEYFYQGGADVEIGSKYSPGVIGHVYAIEAAILGGIKRYDFMKGGTNS
                     YKTEFGCGESPMHDMRVFARTFRGRALAMENWARGHWRSFRRGREDVTDADEIPGSTS
                     SRATPGNGPEQM"
     gene            complement(7963..9036)
                     /locus_tag="AC16FV_12030"
     CDS             complement(7963..9036)
                     /locus_tag="AC16FV_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR2850813.1"
                     /translation="MYLEFFKLKELPFRLTPDSEFLYMSGAHSRAKAYMDYTVWNREG
                     FVVITGEIGSGKTTLIQKLLSELDENVLVAKVFQTQLDEVEFLQAVLVEFGLNPFNAK
                     KVELIDMLNTFLVDNFLQLKQLVLIVDDAHNLSLKVLEEIRMLSGLETRKEKVLHVIL
                     VGQPALSDMLDSPEMEQLVQRVRLRYHIRSLTENDMRDYIHHRLRIAGAEEPEELFTP
                     DTFPIIYKYTGGLPRLINTLCDTAMTCAFADNVRTVSMNVLNTAIEELQWLPYARRIN
                     KRRTEISATGGSEAQGLIRDNTKALANISQQLGRLDNIAPALSSLAGKMASIETLLKR
                     VADSLENAKPDVEGGVSKRARER"
     gene            complement(9044..10423)
                     /locus_tag="AC16FV_12035"
     CDS             complement(9044..10423)
                     /locus_tag="AC16FV_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850814.1"
                     /translation="MQALKVREVMGKYLAFSVLIFSFASPLQAGEFAYGLGYTVSHSD
                     NITRVPSNERSEVTDSYLAGFAYVERTVDLIANVLAQGEFRNYRDDVFGDETIYNVNS
                     SAIWIISPQRFTWTVQDRFADEQIDATAVDTPANRANVNVFSTGPDFYVRFTPVNTLA
                     LGARAGNVSTGSANADNDRFSGSARWLYQATSISTYSLNFQAMDVNYDDSTINTDFVR
                     HDIFLRADYRPSRSRYIVDLGSTKINPDRGQDIDGSLARLSWIRQLTQESAFGISASG
                     EYSDTGTDILTTASELNTASSSAAGIPTISEFPGTTVSGLTSDAVTSDVYYAKRGEIF
                     YNRRGGSLGVDFIASARDLDFETTPQDRKEIDGRLELAFFYSGTTTVTLFTEQLKTDF
                     QNFVREDTERDSGLRFNYRLSRTISAGLEGRRTERTSTVPTEEYEENRVFLRILYSSS
                     PLFSPISSR"
     gene            complement(10426..>10697)
                     /locus_tag="AC16FV_12040"
     CDS             complement(10426..>10697)
                     /locus_tag="AC16FV_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850815.1"
                     /translation="APPMTESADTQILAELCDYAVLVVPYGRVTDAQIDGCIKALGDK
                     KLVGIVFNDEPTLPEFHWWDMLKNPLAAVRAVLSHAGDGRHRRAR"
BASE COUNT         2480 a         3117 c         2969 g         2131 t
ORIGIN      
        1 tccgggaaac accggcactg acggcgcatc tcgattccct tgccatcgca gggggagata
       61 ttttgctgtt acatgaggat tatgcgcata ccgtcgagtc cctgccccgg atactgcaat
      121 cgctgaaaga tcgctccctg aaattttccc ggatcagcga tctcggcagg cacagtgaaa
      181 cctgatcggc cgatgtaccg atcactgttg tttgtcgccg ccctgctggg cctggtctgt
      241 acactccctg ccttgtcgca agatgtcact accaccggca cgacctatta tatttccccg
      301 gccggcaaga actggaacaa cggcaaatcg gccagccagc cattcaaaac ctttgcgcac
      361 gcattcgaca aaatgtccgc cggcgatgag ctggtattac tggacggcgc ctattcggag
      421 ccactcggta cgggttacat cagttatacc gaccccagtc gcggaatcgg aggggcgcac
      481 tcggggcaga ttcccgccgg cgtggatgcc gaccgcgtga cgcgtgtgcg tgccctgaac
      541 cccggaaaag tcaccgtcat cggcggactg gtcatcggcc gctcctttcg caaagacagc
      601 tatataaaaa tccagggcgt caccttcgaa ggcggcggca gcctttacaa taccagccac
      661 gtcacaatca aggactgcgg cttccatggg cccttcagca tcggcaccaa cgatcacaag
      721 gaaggcaacg atttcaatct gatcgaggat gtctggatct gggcctccgg cgaacgcatc
      781 atcgccatca attaccgctc acaattcaac gtctggcggc gtgtgctggt gcgcggcgac
      841 ggctgcaaca agcccgaatg ccgcggcgat tccaatccga atgtcggcat caccgtatac
      901 gattcaagca acgtttccct ccagaatgtg atggtggtgg accgcattct ggcggacaag
      961 gacgaacctt acggggactt tgccgtggcg caacatacgc gcggcgccca tttctttgga
     1021 caaagcgaat ggcttgggac gatctcgctg cgctcgcccg acacgggcta ctacatggaa
     1081 cctgatcacg acggtaccat cgatcccaca atccgcatct ccaacgccat cgcctgggac
     1141 gccgcccagc tgggattcaa cctggcccgc gccggaacca ataacgagct ggagaatctc
     1201 acggtgcgcg cgcgtggcgg cgatggcgtg cgcatcgccc caagcctgac ttccggcacc
     1261 ctgcgcaatg tcatttccgt ggatgcgcta cgcaaaggca tcaattcgcg ctatcctttc
     1321 agtcatgtca atgtttacaa cgcgagacag ggtgcttatc agcagaatag ctgcgatgcc
     1381 ggctgctatt cgagcgatcc acgcgcggac ggcaggattc cttccctgaa atatctggtt
     1441 cgcattgagt ctggctcaaa actcaaaggg agcggatatg aaggagcgga catcggtgcc
     1501 aacgtgctct accgctacgg cgttgatggc tcgcgttacg gtgacctggg cttcaacgca
     1561 cttacgtctg cgccgctctg gccatggccc aacgaggaac gaatcaaaac cgaaatgtgc
     1621 gagaaggccg gagtgacgcg cggcttctgc cgggccagat cgttgacaca ttacatctgg
     1681 gaatatctgg gaaatcccat gccagccgat ccttacgcac catcggccat gccgctgaaa
     1741 acgcaacagt catctaataa cgcccgcggc ggtctgataa gacggtggtt tcagtgaaat
     1801 atttcaggta ggcttaacaa cccggcaaaa taggcgttcg agacatagcg ttcgacatgc
     1861 tgccggcgca ggcgaaaccg gtccatgtcg ccgagcctgt tcacccccgg catgtaggac
     1921 acccccagac gataacccgc ggccgtcgcc gcccgcacca ctctgtcgtt gaactcctcg
     1981 ggcccgccca ccggataggc tagcaccggc gccggcttgc ccagttcctg ctcgagacgc
     2041 agtcttgaat ccgtcagttc ccgccgaagc tcttcgtcat cgaggcgcga cagaatgggg
     2101 tggtttacgg tgtgcgagcc gaattcaatg ccggccgcgc tcatttcacg gatttgatcc
     2161 cagctcagcg ggcggctcag gcgaaaatcg gcgggccgca tggcatcgcc atgctcgcgc
     2221 tcaagccagt ccagaatgcc gagacgttga gcgttgccga cgcgcttgag cgcgccgacc
     2281 aggcggcccg ccgcgatgcg ccttgagttc acatcggtat ccagtggcaa cgtaataccc
     2341 agatctttca ccgtcaggtt tccgttcggg aggtgataaa ggatatgagt cgccagatcg
     2401 aaccagaagg gcttgccact gccgacatag ccggtggaaa cgaatatggt cgcgggcatt
     2461 ccgagcgacc ggagcacggg aaaggcattc agataattat cgtcgtagcc gtcatcaaac
     2521 gacacgatca cggggcgcga aggcagttgc tctttgccgt ccatggcgtt caacagtgtc
     2581 tgaaaggtga tcggatgata ccgtttcttc agatatagca tctgccaggc aaacgccggg
     2641 gaagaagcgc tgacaagatc ggggtcgaat gaaaatgcct gttcctgccc gatgtcgaag
     2701 acgcgatgat aggcaagaat gggtatctca ccggccctca agccacgcag aaaggaagac
     2761 aggccgtgca atccggttat ggccatgccc cgcgccagat attctttttt tctcatgtca
     2821 gataatgcgg cgccgacaaa gcggcgcctg tctccccgtc agacataaag catgacaaac
     2881 accttgacaa gtatggccca accgacggtc gtcatggcga tgacccagaa atcccgctcc
     2941 gtgaacagat tcggctgcct caacctgaaa cccagggcgg cacagaggcc cagcaggaag
     3001 tagagtgttt catactccag cgtgacaaac atggaactga cgatgtaccc caccacgctc
     3061 agtcccagcg cggtggcgta ggccctgtcc ctttcgttct cgcattcacg cacgtaatac
     3121 acgacgtttt tcagcgccat gtagagcagg ccgatccaga aaaacagccc gggaaggccg
     3181 gtttcaccca tgatctcgat gctggaatta tgggcgatga gttttcctgt gtaattgcga
     3241 aaattgccct tgccgatgcc aaacagggga ttgtactgga ccatctccac gccttcgatc
     3301 cacatatcga tgcggtgttt ggtcgacttg ctcggatcgt ccagcgtcgt gatgtagctg
     3361 ggcgccagca tgaaggccgc cgaaatcacg ccaccgacaa aagcgatctt gatcagcgat
     3421 accttgtaac gcacagcaac aaagagcgcg aatatcgtga gtgttgtcag gaaaccgccg
     3481 cgcgaacccg tgaaatagat cgctaccagc aacatgccga gcaatcccgc acccaatagc
     3541 ctggttccag cgctaaacgg cgccacaaaa tattgcatca cgaaaggcaa ggcaatcgta
     3601 tagataacgc agaaaacgcc gggcccgtca aaaatattga tccagcgcgt gcggcccggc
     3661 tcgccggccg cggcggcttc gggatcaatc cagcccagcg cttgtcccgc ccaccccaga
     3721 cccgactcac tggtcttgtg ttcgatgcct tccatcgcaa gcacgagacc gaaaaaaaca
     3781 agcatcagcg ccaccgccct caaacgcccg gcggaggtca ctgtcatgga gaccagtttg
     3841 aacagcatga accatttggt gtaattgaca atgatgtcca tgctgttatc ttgaaatccg
     3901 tttaccgcca tggacaggat tatccacagc agcatcaaca ggaaaaactt gtcgagggac
     3961 gtgaaggtaa gcggattctt ttccctggcc agaaaggccg ccaacaacca cagggggtaa
     4021 acgaagaagt ccacgcgcag gcccacgaag ggttcgaccc agagttgggg cgcgataaaa
     4081 agcgccgtca gatatatgaa gataatcggc acaacgacgg tcctgtgatg gttacacctt
     4141 aaatatccct gcgctgaatc agccgcacca gcgccgaccg cagccaggct gtttccgtgg
     4201 cgtgcaaggc gccggagaaa aacaacgcga ctataaacgt gacatagagc aggatttttg
     4261 cgaaaaaggc cacaaaaatc tgctgacctt caagcaatat ggacagcagg tagaaaattc
     4321 cggtaacggc ggcaatcatc gccagccggc gataatcgta gctaaccctg aaataagcct
     4381 gggaaatacg gttggtgacg accagcacca ccgccgcggt cagaacctgc gccagacagg
     4441 cgccgaccag gcctagccac ttgatcatca gaaaattcga tgtgacgctc gtcaccgccg
     4501 cgatcaggcc gatatggaaa atatggcgtg ttttcttttc atagagaata ccggtttgaa
     4561 acgggtaatt cagcaccttg agcagaccgg cgaccatcag gacaggcagg ataccggcgg
     4621 ccggccaata ggcggcatcg gacatggccc tcagcagatc gttcgcgaaa tagacaatgc
     4681 cgagaccgac ggcgcacagt cctatcaaca ggtaacggac gatgcgcgcc tgaatctcgg
     4741 ccgcattgcc ctccttcatg atggaaaacc ggaaagcccc gtaagaccgg ttaaaaggtt
     4801 cgccaatcag actctccagc agttcggaga acttgagggc gagcgcgtat atgccgagta
     4861 tctgcaggct caggaggctg ttgacgagaa accggtccgt gtacgtggag accagcgcgg
     4921 tgatggtgct cagcaaaaac gggaaactgt atttcagggc cgggaccgcc ttgctccact
     4981 cgaagcggta cccgcacttt ttcagaacga aaatcgtgag acaaccccag ccaaaggcga
     5041 cggccagaaa gttcccggcg agcacgcctt cgacgcccat gttacgaaaa accacaaagt
     5101 aggtgttggc ggcaagctgg atgacaagct tgaaaaacat gatgccaaca aaaagcattg
     5161 aatattccct ggcgcgcaga taggccaggg acacctgcga ggaaagctca agcaccaggg
     5221 tggcgagaat gatcaatatt ccgcggctgt attcgggacc tccaaacacg taccgtgcca
     5281 gggcttcatg ccactggcca accagcagaa ccccgatgga ggtgatcaac agggaaaccg
     5341 tcaggctggt cgtcaccacg gaatgccggt cggtctcggc gtcatgttca aagtagaacc
     5401 gcagtgtcgc gtgcgcgatc cccactgaga gaaaaccggt gaccacggcg gcaacgacgt
     5461 agaagagttc gagcgcgccg tattcagtga ccgtgaggta attcgtgtaa accggcagga
     5521 gcagcaatgc cgcgatgcgg ttcagcacgt tgccaaacgc gtaaatcgtc gagtgcttga
     5581 agaaccgccc taatgacgcg ttttcggatg ccaagatctg ttccgtcgaa aaatgattac
     5641 gtggtgctca agatagcgcc tacagcaagg cctgactccg aagcacgtcc tccatggtcg
     5701 tgaacgagaa atccttcagc aggcgatcga gtcgggggcg ggtccgggcc agattgagat
     5761 aatgccgcaa ccgcgacatc gcgccggcct cgcggatgcg gggttgcccg ggatcgactt
     5821 cccagggatg aagataaaat acgaattcat gtccggagcg gttgatttgc gcgagacccc
     5881 agcgcgtaag aggatacggg aacagacgga aatagccacc acccgccaca ggcagcttga
     5941 acgcccccga acgaaacacg gagatgggaa attccgtcag cgtgtagccc gcgggcgtgc
     6001 gcagccggtt cggatatggt tgcgcgtcgg ggattccgta gcggtcgtgg cgcatcggga
     6061 aaatgcttga atcgtaccgg aatccctctt cgtgcaggat atccagcgcc cacagggatt
     6121 tgtcggtgat cgaataagtc gcggcacggt atccgatgac cgggcgctgg cagatatcct
     6181 cgagcaacgc cttgctacgg cgcgtctcgt cgcgaaatac gtccggcgtc tgggtgtaca
     6241 ccagggtgtg gctcataccg tgcgaagcaa tctcgtggcc acgatcggcg atttcacgga
     6301 tgagagcgcg ctgatgttgc gccacccagc ccagcacgaa aaacgttcct ttgaccgaat
     6361 actcgtcgag cagatcgagc aaccggcggg tgttgtccgc tacccgcgat tcgtaacttc
     6421 cccaggtatt cttgttcacc acgccggcga acgcctccac ctgataataa tcttccacgt
     6481 cgattgtaaa tgcgtttttc atgcgaacag gttcgatgtc ctgatggggc tattgccggc
     6541 gaataccgat tgttcttgat ttaggattgc ccgttcttga taagtatttg caattcagca
     6601 tatcacattt gttccgggcc atttcctggc gtcgctcgag aactcgtact tcccggaatt
     6661 tcatcggcat cggtcacatc ttcgcgtccg cgcctgaatg aacgccaatg accacgcgcc
     6721 cagttctcca tggccagagc tctcccgcga aacgtcctcg caaacacgcg catgtcgtgc
     6781 atcggactct ccccgcaacc gaattcggtt ttgtatgaat tggtcccgcc cttcatgaaa
     6841 tcataccgtt tgatcccccc aagaatcgcg gcctcgatgg catagacatg cccgattacg
     6901 ccgggtgagt atttggaccc aatctcgaca tcggccccgc cctgataaaa atactccgtg
     6961 cccgcgtgac gcaggttgta atgggcggcg atgttcacgc cgtccagcga caggaagccg
     7021 agattgagca ttccctgcgg gagcaggatt ttcgccagac gccggtgata gtcggcgaag
     7081 cgcgaactgg caaacacacc ctccctgcct cgcgccttcc agcgcttggt gtgcagccga
     7141 atcagctcat cgaaggcctg atccagctcc tccgcttcgg taacactctt gtactccaca
     7201 cgacccgcgc gctcgaactt gcgccgtttg taggggagcc gcttggcagc cccgtcatcc
     7261 agcatggcga tatattcctg ccacgtcggt ggcagcgcaa cgctgtaacg gattcccacc
     7321 ttctccaggg ccacgggaat tctttcggca gtcaggacgt gtcgcagtgt tgtcaaaacc
     7381 agcgaaccct ccagcacgtc attgaataca agctggtccc agaactcctg gtcgcgtaaa
     7441 tgcgcccaca ctgcttttgc cacttggtcc tcgtatccgg tcaacgcaac gatatccaga
     7501 tattcagagg caacttcctc ctgctccggt tctccggttc cgagaaaacg cagcgtgcga
     7561 atcgaaccca ggacgggttt cttttcgatc tggagataaa aaggcgcgag ccccaccagt
     7621 cgggcgtcat cccgcaccaa caatacgcag ggttttacgc cgcaatcatg atagatatcc
     7681 caccaggtat ggatccattc ccaacgcagg caaatactgc gtgcactgga ggcatcaagc
     7741 agatgattcc actcatcccg caggttcatg agacccgcgg acgaatcaac cacggataga
     7801 gtgatatctt ttcccatgcc taggatgctg atcgggagac agaaagcaac gcaagggggt
     7861 cgtcaaaaat gaatgatccg gtgactggaa cgtcggggag aacaacctgc gcccgccaaa
     7921 tacgcggcct ggacaacgca tgggcagacg cgagcggtag aatcagcgct ccctggcgcg
     7981 ctttgacaca ccgccctcga cgtccggttt cgcattctca agcgaatcgg caacgcgttt
     8041 aagcaatgtt tcaatgctgg ccatcttgcc ggcaagggag gacagcgccg gagcaatatt
     8101 atccagtcgg cccagttgtt gactgatgtt ggccagggcc ttggtattat cgcgtatcaa
     8161 gccctgtgct tcgctgccac cagtggcgct gatttccgtg cggcgcttgt tgatccgtct
     8221 ggcatagggc aaccattgca actcctcaat ggccgtgttc agaacgttca tcgacacggt
     8281 gcgcacgttg tcggcgaagg cgcaggtcat ggcggtatcg cacagggtat tgataagccg
     8341 cggcaacccg ccggtgtatt tatagattat ggggaacgta tcgggcgtga aaagctcttc
     8401 gggctcctcg gcgccggcga tgcgcaggcg atgatggata tagtcgcgca tatcgttttc
     8461 cgtcagcgat ctgatgtgat accgcagacg cacgcgctga accagctgct ccatttccgg
     8521 agagtccagc atgtcgctca gggccggctg ccccacgagg atgacgtgca acactttctc
     8581 cttgcgcgtt tccagccccg acagcatgcg gatttcttcc agcaccttga ggctgaggtt
     8641 gtgcgcgtca tcgacgatca gcaccagctg cttgagctga agaaagttgt cgaccagaaa
     8701 ggtattgagc atgtcgatca gctcgacctt tttggcattg aagggattga ggccgaactc
     8761 aaccagcacc gcctgcagga attcgacctc gtcgagttgc gtctggaata ctttcgccac
     8821 cagcacgttc tcgtccagtt cggacagcag cttctggatc aaagtggtct tgccgctgcc
     8881 gatttcgccg gtaatcacga caaacccttc gcggttccac acggtgtaat ccatgtaggc
     8941 cttggcgcgg gaatgcgccc cgctcatgta caggaattcc gagtccggcg tcagccggaa
     9001 cggcagttct ttcagcttga aaaactcgag atacatcgtc gtgtcacctc gacgagatgg
     9061 ggctgaacaa cggactgctg ctatagagga ttctcaaaaa cacccggttt tcctcgtatt
     9121 cttccgtcgg cacagtgctg gtgcgttcgg tacggcggcc ttccagtccc gcgctgatgg
     9181 tccggctcag gcgataattg aaacgaaggc cggaatcgcg ttcggtgtct tcccgaacaa
     9241 aattctgaaa atccgtcttg agctgttccg tgaacagcgt caccgtggtc gttccggaat
     9301 aaaaaaaagc gagctccagg cgcccgtcta tttccttgcg atcctgcggt gttgtctcaa
     9361 aatcaagatc gcgcgcgctc gcgatgaagt ccacgcctaa actgccgccg cgccggttat
     9421 agaagatttc tccgcgcttg gcgtaataca catcgctggt gacggcatcg gaagtaaggc
     9481 cgctgaccgt cgtgccggga aattcggaaa tggtgggaat gccggccgcc gaagacgacg
     9541 cggtattgag ctcggacgcc gtcgtcagaa tatcggtgcc ggtgtctgaa tactccccgc
     9601 tggcggaaat accgaaggcg gactcctgcg tcagttggcg gatccaggac aatctggcca
     9661 gggatccatc gatatcctga ccgcggtcgg gattgatctt ggtagagccg agatcaacga
     9721 tgtatcgcga acgcgaggga cggtaatcgg cgcggagaaa aatgtcatgg cggacaaaat
     9781 cggtattgat cgtggagtcg tcataattga cgtccatggc ctggaaattg agcgagtacg
     9841 tggagatgct cgtcgcctgg tacagccagc gggcggatcc gctgaaacgg tcattatcgg
     9901 cattggcact tcccgtggat acattgccgg cccgggcgcc aagcgccagg gtgttcaccg
     9961 gcgtgaaacg cacataaaaa tccgggccgg tgctgaaaac attgacgttg gcgcgattgg
    10021 ccggcgtatc caccgccgtg gcatcaatct gttcatctgc aaacctgtcc tgcacagtcc
    10081 aggtgaaccg ctgtggggaa ataatccaga tcgcggaaga attcacgtta taaatcgtct
    10141 catcgccgaa aacatcatcc cgatagttgc gaaattcccc ttgggccagc acgttcgcaa
    10201 tcaggtcgac cgtgcgctcg acataagcga agcccgccag ataggagtcg gtcacctcac
    10261 tacgctcatt cgacggtacg cgcgtgatgt tatcgctgtg cgagaccgtg tatcccaacc
    10321 catacgcgaa ttcgcccgcc tggagtggcg aagcgaaact gaagataaga acagaaaacg
    10381 ccagatactt tcccataacc tccctgacct ttaacgcctg catttttatc tcgctcttct
    10441 gtgcctgccg tcgcctgcgt gggaaaggac agcccggacg gcggcaagcg ggtttttcag
    10501 catgtcccac cagtggaatt ccggcagcgt gggctcgtca ttaaatacaa tgccaaccag
    10561 cttcttgtct ccgagcgcct tgatgcagcc gtcaatctgg gcgtcggtca cacggccgta
    10621 aggcaccacc agcacggcgt agtcgcacag ctccgcgaga atctgtgtat cggccgattc
    10681 cgtcatgggc ggtgcat
//
LOCUS       DBNGZE010000463         1766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_133451, whole genome shotgun sequence.
ACCESSION   DBNGZE010000463 DBNGZE010000000
VERSION     DBNGZE010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_133451"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16FV_12045"
     CDS             <1..375
                     /locus_tag="AC16FV_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR2850816.1"
                     /translation="HNARVLINGDEALARRSGADGVHLTSMQLMQRKERPATPFFGAS
                     CHDAAELTQAARLSADFVVLSPVLPTPTHPEAGGMGWDKFASLVSDYPVPVYALGGMK
                     PELLDTAMKHGAHGMSLLSGIW"
     gene            383..634
                     /locus_tag="AC16FV_12050"
     CDS             383..634
                     /locus_tag="AC16FV_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HIR2850817.1"
                     /translation="METNTLGLIAAALTTAAFIPQVVKIWRSRHTKDISLGMFAIFSV
                     GVFLWLLYGIRIDALPVILSNGITLVLSLTILFFKLKYK"
     gene            707..1612
                     /locus_tag="AC16FV_12055"
     CDS             707..1612
                     /locus_tag="AC16FV_12055"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR2850818.1"
                     /translation="MPEPLFQSDLKSLRLRHRGKVRDIYDIDQSHMLIVTTDRLSAFD
                     VVLPDPIPGKGALLTAMSNFWFNRTRHLISNQLSDMPIEKALKDPAERKQVEGHATVV
                     RKLKALPVEAIVRGYLVGSGWKEYQKNGTVCGIKLPAGLKEADKLPEPIFTPSTKAAV
                     GDHDENISLEKAAEILGKDIAEKVKHAAIAIYKECAQYALTRGIIIADTKFEFGLDEK
                     GTLTLIDEVLTPDSSRFWPADSYKPGANPPSYDKQYVRDYLESIGWNKKPPAPKLPPE
                     VIAKTAEKYREALTRLTGSEIAARC"
BASE COUNT          385 a          498 c          525 g          358 t
ORIGIN      
        1 cacaacgcca gggtacttat taatggcgat gaagccctgg cccggcgcag cggtgccgac
       61 ggcgtgcacc tgaccagcat gcaactgatg caaagaaagg agcgaccggc aacgccgttc
      121 tttggcgctt cctgccacga tgccgccgag ctgacgcagg cggctcggct ttcggccgat
      181 tttgtcgtgc tgtcaccggt attgccgacg ccgacccacc ctgaagccgg gggcatgggt
      241 tgggacaagt ttgcgagcct cgtgagcgat tatccggtgc cggtgtatgc gctcggcggc
      301 atgaagccgg agttgctcga caccgcgatg aagcacggcg cccatggcat gagcctgctg
      361 agtggcatct ggtgaggaaa tcatggaaac caatacactg ggtttgatcg ccgcggcgct
      421 gaccaccgcc gcgttcattc cgcaggtggt gaaaatttgg cggtcccggc acacgaagga
      481 tatatccctg gggatgttcg cgatcttcag cgtgggcgtg tttttgtggc tgctgtacgg
      541 catccggatc gatgccctgc cggtcattct gtccaacggc atcacgctgg tattgtcgct
      601 gaccattctt tttttcaagc tcaagtacaa ataatctgcc ggggttgaga ctgttttcca
      661 gagccgatag catcgaacca ccattgctgc atcgtcggag cgatacatgc cagaaccatt
      721 atttcaatcg gacttgaaaa gcctgcgcct gcgccatcgc gggaaggtgc gcgatattta
      781 cgatatcgac cagagccaca tgcttatcgt taccaccgac cggctgtcgg ccttcgacgt
      841 ggtgctgccg gatcccattc ccggcaaggg cgcattgttg accgctatgt cgaatttctg
      901 gttcaaccgg acgcgccatc tcatttccaa tcaactgtcc gacatgccga tcgagaaagc
      961 gctcaaggac ccggccgagc gcaaacaggt ggaaggccac gccacggtgg tgcgcaagct
     1021 caaggcgttg ccggtggagg ccatcgtgcg cggctatctc gtcggcagcg gctggaagga
     1081 gtaccagaag aacggcacgg tctgcggcat caaactcccc gcgggtctga aggaggccga
     1141 caagttgccc gagccgatct tcaccccgtc caccaaagcc gcggttggcg atcatgatga
     1201 aaatatcagt ctcgaaaagg ccgcggaaat tctgggcaag gacatcgccg agaaagtgaa
     1261 acacgcggcc atcgccatat ataaggagtg tgcgcagtat gccctgacgc gcgggatcat
     1321 catcgctgac accaaattcg agttcggtct ggatgaaaag ggcacgctca cgctgattga
     1381 cgaggtgctg acgccggatt cctcgcgttt ctggccggcg gattcataca aacccggtgc
     1441 caacccaccg agttacgaca agcaatacgt gcgtgattat ctggaaagca tcggctggaa
     1501 caagaagccg ccggcgccca agctgccacc ggaagtcatc gccaaaacgg cggagaaata
     1561 ccgcgaggcg ctgacgcgcc tgaccggcag cgagatcgcc gcgcgctgtt aggtagttct
     1621 tgatcttggg aaagcgacca gcggcacgac atttcgtctt ttttaatgat ggagcttgat
     1681 tcagctatat ccggttagac atttttcagg gcagacaatg gctgattgtt gcaacgacaa
     1741 agcttgcgag atcgaggcac ttcgtg
//
LOCUS       DBNGZE010000464         4134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1313829, whole genome shotgun sequence.
ACCESSION   DBNGZE010000464 DBNGZE010000000
VERSION     DBNGZE010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1313829"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="AC16FV_12060"
     CDS             <1..1581
                     /locus_tag="AC16FV_12060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850819.1"
                     /translation="SPNSYLLLASVVALLWVMHPIQLTSVLYVVQRMTELSGLFTLMA
                     LICYLTGRLRMVTSKPGGIRLILVGSVFFGTLGIFCKETAVLLPAYMLVFELTLFSTE
                     HPWSHWQKLPVKMKGTLLLSVAIAGIVMLTWAIHYSLPKYAIRDFSMVERLLTESRIL
                     FFYLSLILVPRLDQFSLFHDDIQLSTSVLSPWTTLPSLVGIVFLLILAYQARRHQPLL
                     SLGIFWFFAGHLLESTILPLELAHEHRNYLPSLGPILVIVHIVNATCIKLQRRWIWGI
                     LPLLVVVVAGVSYIRATQWSSDYSLFSYETSHNPKSASIVSGYALRLIEVGKYQEGMQ
                     AFRRAAHLEPEEASYLIYMNMFAANAGEKFDQAGAEETLKRLATGHITATTGQALQYL
                     GDCVLSWCHSLGPSMELWMKTLLNRENQIGVDRSYFNYLLARSLFAQGKVNEAIKIYN
                     LAHETDKNYLHPLFDLVYLYARKGDGDKAMETIRRLKDANRTARHQRTDQIEETEKDI
                     EKLLNRPPESPGKAKQKR"
     gene            1578..3581
                     /locus_tag="AC16FV_12065"
     CDS             1578..3581
                     /locus_tag="AC16FV_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850820.1"
                     /translation="MNVSWRQASSKKLLAVLALVVAVIVVYFPGLYGPFVFDDEIHVL
                     SDPSIRISSLNFQSLTNAVISEDGSLFQRPLARITLAINYYLSGGASSALGYKLTNLA
                     IHIVNTGLVYWFSLLLLRQQASRPKAIGKKALEWAPLLVAALWALHPLHLTTVLYVVQ
                     RMTSLAAFFVLTGLIVFIHGRQRVHEQRAHGYLLMAMGAIGGSTLGLACKENAILLLP
                     LMLVIEYIFFSASSEAPSDRRKLLLFYGLTVLMPALLLGLWLITHPDIILDAYRAREF
                     TLVERLLTESRVIWFYIGLLFLPDTRKLALYHDDIPISTSLFDPWTTSIAILSIFLVV
                     IFAIMARRRYPIFSFAVLWYLVGHGMESGVIGLEIAHEHRNYLPDIGILFAVAYGLFV
                     AALRFQRYMRYMILLAPVITLGFVTNTLAYTWASDESLIKALARYHPDSARGQYMLAE
                     LYAEKEHDLLAALVHYRRAAELAPNETGYLVKSAITAARMNPGQRHDAGNTPGRSKDS
                     ESIDRQLKEQPLTSSTLYILEQLASCTDQTSESCKLLYPEIKGWYLAVVQNPRISGKN
                     RGNFVVYLFNLGAAHPDLDLSLQAARWGKKFEPSNPDYSLMEANVYLLRGDLETAENL
                     LQKMMLENSAIGLTSETRDNIAVLLGEIESRKSRVRSNLNSRK"
     gene            3695..>4134
                     /locus_tag="AC16FV_12070"
     CDS             3695..>4134
                     /locus_tag="AC16FV_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850821.1"
                     /translation="MILLIYFPGLSGPYVLDEGENSANNEALAVEEINPANLWRAMQS
                     NDSGLFRRPLATLTFAFNHYLAGGFDNTFPFKLTNLVIHAANGILLLFLTLTILRGSD
                     AGRKLSSTEQLGVAALTAVLWSAHPIQLTNVLYVVQRMNSLSAL"
BASE COUNT          936 a         1148 c         1024 g         1026 t
ORIGIN      
        1 agcccaaaca gctacctgct gcttgcctcg gttgttgcgt tactgtgggt gatgcatccc
       61 atccagctaa ccagcgtgct ttatgtcgtg caacgcatga cggaactttc aggactattc
      121 accctcatgg ccctgatctg ctatctcacc ggccgactca ggatggtgac gagcaaaccg
      181 gggggcatcc gattgatttt ggttggttcc gtcttcttcg gaacactcgg aatattctgc
      241 aaggaaaccg cagttttgct tccggcatac atgcttgttt ttgagttgac cctattcagc
      301 accgaacacc cctggagcca ctggcagaaa ctgccggtaa aaatgaaagg cacactgctg
      361 ctgtctgtcg ccatcgccgg tatcgtcatg ctcacgtggg caatacatta ctcccttccc
      421 aaatacgcca tccgtgattt cagcatggtc gaaaggctgc tgacagaatc acggatactt
      481 tttttctatc tctctcttat tctggttccc cgtctggatc agttcagcct gtttcacgac
      541 gacattcagc tttccacgtc tgttctgtcg ccctggacga ccttaccttc tctggtcggc
      601 atagtattcc tgctgatcct ggcttatcag gcacgccgcc accaaccgct actgagcctc
      661 gggatattct ggttcttcgc cgggcacctg ctcgaatcga cgattctgcc gctggaactg
      721 gcgcacgaac acagaaatta ccttcccagc ctcggcccca ttcttgtcat cgttcacatc
      781 gtcaatgcca cctgcataaa attgcagcgt cgctggattt ggggcattct gcctttgctc
      841 gtggtggttg tggcgggagt cagttatata cgcgccacac aatggagcag cgactattcc
      901 ttgttctcct acgaaacctc gcataacccc aagtcagcca gtattgtatc cggatatgcc
      961 ctcaggctga tcgaggtagg caaatatcaa gaaggcatgc aagctttccg gcgcgctgcc
     1021 catctggaac cggaggaagc ttcctatctt atatacatga acatgtttgc cgccaacgcc
     1081 ggagagaaat ttgatcaggc cggcgccgag gaaacactca agcgtctggc aactggccat
     1141 atcacagcca cgacaggaca ggcgttacaa tatttaggag actgcgtcct gtcatggtgc
     1201 cattccttgg ggccctctat ggagctatgg atgaaaacgt tgctcaatcg ggaaaatcaa
     1261 atcggagttg acaggtctta tttcaattac ctgctggcac gctcgctttt cgctcaggga
     1321 aaggtcaatg aggcgataaa gatctacaat ctggcgcatg aaacagacaa gaattatctg
     1381 catccgttat tcgatcttgt ttatctctac gcacggaaag gcgatggcga caaggctatg
     1441 gagacaattc gcaggctgaa agatgccaat cgtaccgccc ggcaccagcg caccgatcaa
     1501 atcgaggaaa cggaaaagga tatagaaaag ctgttaaatc gtccgccgga aagtccagga
     1561 aaagcgaaac aaaaacgatg aatgtctcct ggagacaggc ctcatccaag aagctcctcg
     1621 ctgttctcgc acttgtcgtt gcagttatcg ttgtctactt tccggggtta tatgggcctt
     1681 tcgtcttcga cgacgaaatc catgtcctgt ccgatccctc tatcaggatt tcttctctta
     1741 atttccagag cctgacaaac gccgtcatat cggaagacgg ctcgttgttc caacgccctc
     1801 tcgccagaat caccttggca atcaattatt acctgtcggg aggcgcgtca agcgccttgg
     1861 gatacaaact caccaaccta gccatacata tcgtaaacac cggcctggtg tactggttct
     1921 cgttattatt gctccggcaa caggcctcgc gcccgaaagc tatcggcaaa aaagcactcg
     1981 aatgggcacc gctgctggta gccgcgctct gggcgctcca cccgctgcat ctgacgaccg
     2041 tgctttatgt cgtgcaacgc atgaccagct tggccgcatt tttcgtgctg acgggcttga
     2101 tcgtttttat tcacgggcga cagcgcgttc acgaacaacg cgctcatggt tatctgctga
     2161 tggccatggg cgcgatcggg ggatcaacgc tggggctggc ctgcaaggaa aacgcaatct
     2221 tgctccttcc actcatgctg gtcattgaat atatattttt cagcgcgtcg tcagaggccc
     2281 cgtcagacag gcggaaactc ttgctgtttt atggtctgac cgtattaatg ccagcgctgc
     2341 ttctgggtct gtggttgata acccatcccg acattattct ggatgcctat cgggcgcgcg
     2401 aatttaccct ggtcgagcgg ctgctgaccg aatcgcgcgt tatctggttc tacatcggcc
     2461 tgttatttct tcccgacacg aggaaacttg ccctctatca tgacgatatc ccgatcagca
     2521 ccagcctgtt cgatccctgg actacgtcca tcgccatatt gtcgattttc cttgttgtca
     2581 tcttcgccat catggcaagg cgaagatatc ccatcttcag ctttgcggtt ttatggtatt
     2641 tggtaggcca cggcatggag tccggcgtca taggtcttga aattgcgcac gagcaccgta
     2701 attatcttcc agacataggc atattattcg cggtggctta tggcctgttc gtggctgctc
     2761 tgaggtttca gcgatacatg cgctacatga tattgctggc gcctgtcatt acgctcggtt
     2821 ttgtaaccaa cacactggca tatacctggg cgagcgatga aagccttatc aaggctctcg
     2881 cccgctatca tcccgactct gccaggggtc aatacatgct ggccgaactc tacgcggaaa
     2941 aagaacacga tctcctcgcc gcgcttgtgc attaccggag ggccgctgaa cttgcgccga
     3001 atgaaacggg ttatcttgtc aaatcggcga ttacggccgc cagaatgaat cctggccagc
     3061 gtcacgatgc cggcaataca ccaggtcgtt cgaaggattc cgagagcatt gatcgtcaac
     3121 tgaaagaaca accgcttacc tcgtccacct tatacattct tgagcaattg gcgagctgta
     3181 ccgatcagac ttcggaatct tgcaagctgt tatatccgga gataaagggc tggtatctcg
     3241 cggtggtgca gaacccccgt atcagcggca agaatcgcgg caatttcgtc gtttatcttt
     3301 tcaatctcgg agcggcccac cccgatctgg atttgtcctt gcaggcagcc agatggggaa
     3361 agaaattcga gccatcgaat ccggactata gtctcatgga ggccaatgtt tacctgcttc
     3421 gcggcgatct ggaaacagcc gagaacctgt tacaaaaaat gatgctggaa aattccgcca
     3481 ttgggctgac gagcgaaacc agagacaaca tcgcggtttt gctcggggag atcgagtcgc
     3541 gcaaaagcag ggttcggtca aacctgaata gtcgcaaatg acgtcgcgcc gaatcaatcc
     3601 gatggaagca atggacgcgc atcagattaa aaatcacccg cagcggctga attaccgcct
     3661 gtcaatcagt cttgtcctga cggctctcgt cgccatgatc ctgctgattt attttcctgg
     3721 tttgtccggc ccatacgttc tggatgaggg ggaaaacagt gccaacaacg aggcgctggc
     3781 tgtcgaggag ataaacccgg caaatctctg gcgcgcgatg caaagcaacg acagcggcct
     3841 cttccggcgt ccattggcca ctctcacttt tgccttcaat cattatcttg ccggcggttt
     3901 cgataataca tttcccttca aattaacgaa cctcgttatc cacgccgcca acgggatcct
     3961 gctgctgttt ctgactctga caatactgcg cggctccgat gcgggccgga aactgtcatc
     4021 cacggaacag ctcggcgtgg ccgcgttgac agcggtgcta tggtccgcgc atcccataca
     4081 gctcacgaat gtcctctatg tggtccagag aatgaacagc ctgtcggcat tgtt
//
LOCUS       DBNGZE010000465         4728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_828036, whole genome shotgun sequence.
ACCESSION   DBNGZE010000465 DBNGZE010000000
VERSION     DBNGZE010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_828036"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="AC16FV_12075"
     CDS             complement(<1..1392)
                     /locus_tag="AC16FV_12075"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR2850822.1"
                     /translation="MEDWLAAIASGRPEPEQKILRQAVALASRAHAAQQRASGEPYVQ
                     HSLAVARILADLGLDHETLAAAILHDVVEDTGIALDDLKNEFGASIAGLVDGVTKMKV
                     IQEFQSEPAGSRREHAQAESLRKMLLAMAEDVRVVFIKLADRLHNMRTLSALPEEKQK
                     RIARETMDIFAPLANRLGIWQMKWELEDLAFRYLEPETYRQTADFIAERREDREAYIA
                     RFVETLSRELSDSGIKAEVTGRPKHIYSIWRKMQNKAKHFEQIYDVRAVRVLVDSIRD
                     CYAALGVVHTLWQHIPGEFDDYIATPKENHYRSIHTAVIGPHGKTVEVQIRTHEMHQQ
                     SELGVAAHWRYKEGVKRAGGKDHAFDEKIAWLRQMLAWRDEIVDASDWVDQSKRAALD
                     DDVYVLTPQGRVIDLPQGATPVDFAYALHTDIGHRCRGAKVNGAIVPLDYRLKNAERV
                     EIITAKSGGPSRDW"
     gene            1588..2199
                     /locus_tag="AC16FV_12080"
     CDS             1588..2199
                     /locus_tag="AC16FV_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR2850823.1"
                     /translation="MKSCKTTLTLFAAMLIAGFFLTGVAQASRTTPMVETTPEAIDPE
                     LRALVKQAALETDSFHDRFDGEVWLADMSQRLERRVPDPAFRVELLKNVHYEARRAEL
                     PPELVLAVIEVESNFSQYAISVAGARGLMQVMPFWLKEIGGPGDSLFRIQTNLRFGCT
                     ILKYYLQKERGNLHAALKRYNGTRESKYSFKVDKALKTRWFPQ"
     gene            complement(2202..2474)
                     /locus_tag="AC16FV_12085"
     CDS             complement(2202..2474)
                     /locus_tag="AC16FV_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR2850824.1"
                     /translation="MNRCRHFLVSGQVQGVFFRASTESTARRLGLTGWVRNLRDGRVE
                     LVACGEEAPLKELERWLWQGPPRARVTQVEVKETVAPSFTGFSVRG"
     gene            complement(2643..3146)
                     /locus_tag="AC16FV_12090"
     CDS             complement(2643..3146)
                     /locus_tag="AC16FV_12090"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR2850825.1"
                     /translation="MFQRIRWVAVVLMMVPVLALAAGPDIRLKDIEGKEHNVNEYIGQ
                     GKWTVVVAWSADCPICRRDIYHMTFFHDEHRKKNATVLGLSVDGFDNRAKARGFIEDQ
                     SLNFPNLIGEPEDASRLSDAMFIGTPTYYFFSPEGKFMAQRIGPVTQQQAEQIIRDLE
                     KQKTSKR"
     gene            3250..4656
                     /locus_tag="AC16FV_12095"
     CDS             3250..4656
                     /locus_tag="AC16FV_12095"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR2850826.1"
                     /translation="MNKKFRTEKDTLGEYPVPHDAWYGIQTARAVENFPISGRQPDPD
                     FIIAHVRIKRAAAAVNESAGALEPRLAKAIVEAADAIIEGKYHEEFVVDRFQAGAGTS
                     HNMNTNEVIANLANVALGGKKGEYKPVHPNDHVNMGQSTNDTIPTAIRLAALAKLPRL
                     MAAVNHMADEYETIARREANTVKSGRTHLQDAVPTTLGREFGAYAWILRRCTHQLRAT
                     IPQLCETGLGGSAAGTGLNTVPGYAVHIAEELARLTGEPVRAADDLAAQMQSMHDLQQ
                     LSGAIRALTLELTRIANDMRLLASGPRTGFAEIELPAVQPGSSIMPGKINPVMFEMLN
                     QVCYQVLGQDHAIALMTQAGQLELNVMMPALGSALFDAMDWLTHAVNAATEKGLRGLK
                     VDRDRCRELAHTSVGLATLLNTSIGYSAAAEVAKESERSGRPVRDIVAEKGLMSTAEF
                     DRLVERAAIDGNISPPRR"
BASE COUNT          938 a         1505 c         1377 g          908 t
ORIGIN      
        1 ccagtcccgg ctgggcccgc cagacttcgc cgtgatgatc tcgacgcgct cggcgttctt
       61 cagccggtag tcgagcggca cgatcgcacc atttacctta gcgccgcggc agcggtggcc
      121 gatgtcggta tgcagagcgt aggcgaaatc caccggcgtc gcgccttgcg ggagatcaat
      181 cactcgccct tgaggcgtaa ggacgtacac atcatcgtcg agtgccgccc gtttcgactg
      241 gtcgacccag tccgaggcgt ccacgatctc gtcgcgccag gcgagcatct gccgcagcca
      301 agcgattttc tcgtcgaacg catggtcctt gccgccggcg cgcttcacgc cttccttgta
      361 gcgccagtgc gcggccacgc ccagctccga ctgctggtgc atttcgtgtg tgcgaatctg
      421 cacttccacc gtcttgccgt gcgggccgat cacggcggtg tgaatcgagc ggtaatgatt
      481 ttccttgggc gtggcaatgt aatcgtcgaa ctcgccgggg atgtgctgcc acagcgtatg
      541 caccacgccg agcgcggcgt agcagtcacg gatgctgtcg accagcacac gcaccgcacg
      601 cacgtcatag atctgctcga agtgcttggc cttgttttgc atcttgcgcc atatgctgta
      661 gatatgtttc ggccggcccg tgacctcggc cttgatcccg gagtcggaca gctcgcgtga
      721 caaggtttcg acgaaacggg cgatataggc ctcgcgatcc tcgcggcgct cggcgatgaa
      781 atcggcggtc tgccgatagg tctcgggttc gaggtaacgg aacgccaggt cctccagctc
      841 ccacttcatc tgccagatac cgagacggtt ggcgagcggc gcgaagatat ccatggtttc
      901 gcgcgcgatg cgcttctgtt tctcttccgg caacgcggac agcgtgcgca tgttgtgcaa
      961 ccggtcggca agcttgatga acaccacgcg cacgtcctcg gccatggcca gcagcatctt
     1021 gcgcagcgat tcggcttggg catgctcgcg gcgagagccg gcgggctcgc tctggaattc
     1081 ctggatcacc ttcatcttgg taacgccatc gacgagaccg gcgatggacg cgccgaattc
     1141 gtttttcagg tcgtcgagcg cgataccggt gtcttccacc acgtcgtgga gaatggccgc
     1201 cgcgagtgtc tcgtgatcga gcccgagatc agcgagtatc ctggccaccg ccagtgaatg
     1261 ctggacatag ggttcacccg aggcacgctg ctgggccgca tgcgcccgtg acgcgagcgc
     1321 cacagcctgg cgcagaattt tctgttccgg ctcagggcga ccggaggcaa tggcggcaag
     1381 ccagtcctcc acgccgttcg tccggggcgt gtcagcggtt gtcgttaggg gtgtgggcct
     1441 gaccatgatg atcgtctgtc gtgtgttgat tttttgaatg gggtcgcgac gatgagattt
     1501 aaacgcctat aattccatta cagacagcat gatcaccttc gccggcggga ttgtccattc
     1561 ttcggcgcag cccgttaaac cattagcatg aaatcctgta aaacaacctt aacattattt
     1621 gccgccatgc tcatcgccgg tttctttctc accggcgttg ctcaggcctc gcgaaccact
     1681 ccgatggttg aaaccacgcc ggaggcgatc gatccggagc tgcgcgcgct ggtaaaacag
     1741 gcggcgctcg aaaccgacag ctttcacgac cggttcgacg gcgaagtatg gctcgccgac
     1801 atgtcgcaac gtctggagag gcgcgtgccg gaccccgcgt ttcgcgtcga actgctcaag
     1861 aacgtgcact acgaagcacg ccgcgccgaa ctgccgccgg aactggtgct ggcggtgatc
     1921 gaggtggaga gcaatttcag tcaatacgcg atttcggtgg ccggcgcgcg tgggctgatg
     1981 caggtcatgc cgttctggct gaaggagatc ggcgggcccg gcgacagcct gttccgcatc
     2041 cagactaatc tgcgcttcgg ttgcacgatc ctgaaatatt atttgcagaa agaaagaggc
     2101 aacctgcacg cggcgctcaa acgctacaac ggcacgcgcg agagtaaata ctcgttcaag
     2161 gtcgacaagg ccctcaagac ccgctggttc ccgcaataat ttcaaccacg cacggaaaag
     2221 ccggtaaacg acggtgcaac agtttcctta acctccacct gcgtcacgcg cgcccgcggt
     2281 ggaccctgcc ataaccaccg ctccagttcc ttgagcggcg cttcctcgcc gcaggcaacc
     2341 agctcgacac gtccgtcacg caggttacgc acccagccgg tcaggccgag ccggcgcgcg
     2401 gtggattccg tggaagcgcg aaagaacacg ccttgcacct gcccggagac cagaaagtgc
     2461 cgacaacgat tcattgttaa tattctaaac cgccaaggcg ccagttccag ccatccttgg
     2521 cgcaacaccg cggacttcaa tattccccag cccgcacatc cgtgtgcggg cgctcgccgg
     2581 gacgaagaca cgttaagtgt cttcgtctat tccggctcgc cctggcgtct tggcggtaaa
     2641 aattatcttt tagaggtttt ctgtttttcg aggtcgcgaa tgatctgctc cgcctgttgt
     2701 tgcgtgacgg gcccgatgcg ctgggccatg aattttcctt ccggcgagaa gaaataatac
     2761 gtcggggtgc cgatgaacat ggcgtcactg aggcggctgg cgtcttccgg ctcgccgatg
     2821 aggttcggaa aattcaggct ctggtcctcg atgaatcccc tggccttggc ccggttgtcg
     2881 aaaccgtcca cggacaagcc cagcaccgtg gcgttctttt tccggtgttc gtcatggaag
     2941 aaagtcatat gatagatatc gcgccggcag atcgggcagt cggcagacca tgccaccacg
     3001 acggtccatt tcccctgacc gatgtattca ttgacgttgt gctccttgcc ttcgatatcc
     3061 ttgagccgaa tgtccggacc tgccgccagc gccaggaccg gcaccatcat caataccacg
     3121 gccacccacc tgattcgttg aaacacgcta gcctcctgtt gctgcaccat caaatataaa
     3181 catggatcat gtccggatgc attcccgtag caccgactct ataatccgtc cacacgattg
     3241 gagtccgcca tgaacaaaaa attccgcacc gaaaaagaca cgctgggcga atacccggtc
     3301 ccgcacgacg cctggtatgg catacagacg gcgcgggcgg tggaaaactt cccgatttcc
     3361 ggccgccagc ctgatcccga cttcattatc gcgcatgtcc gtatcaagcg cgcggcagcg
     3421 gcggtcaacg aatccgccgg cgcgctggag ccacgtctgg ccaaggccat cgtcgaggcc
     3481 gccgatgcaa tcatcgaagg taaatatcac gaggaattcg tcgtggaccg gtttcaggcc
     3541 ggcgccggca cctcgcacaa catgaacacc aacgaggtca tcgccaatct cgcgaatgtc
     3601 gcgctcggcg gcaaaaaggg cgaatacaag ccggtgcatc ccaacgatca tgtgaacatg
     3661 ggccaaagca ccaacgacac catcccgacc gcgattcgcc tcgcggcgct cgccaagttg
     3721 ccgcgcctca tggcggccgt caaccatatg gccgatgaat acgaaacgat cgcgcggcgc
     3781 gaagccaaca ccgtcaagag cgggcgcacg catctgcagg atgcagtgcc aaccacactc
     3841 gggcgtgaat tcggtgccta cgcctggatt ctgcgccgtt gcacacatca attgcgtgcg
     3901 accattcctc agctgtgcga gaccggactc ggcggttccg ccgccggaac cggactcaac
     3961 accgttcccg gctacgctgt gcacattgcc gaagaactgg cacgcctcac cggcgaaccg
     4021 gtccgcgccg ccgacgatct cgcggcgcag atgcagtcca tgcatgacct gcaacagctc
     4081 tccggcgcca ttcgcgcatt gaccctcgaa ctgacccgca tcgccaacga catgcgcctg
     4141 ctcgcctccg gcccgcgcac cggctttgcc gagatcgaac tacctgcggt gcaaccgggt
     4201 tcgtcgatca tgccgggcaa gatcaatccg gtgatgttcg aaatgctgaa ccaggtgtgt
     4261 tatcaggtgc tcggacagga tcacgccatc gcgctgatga cgcaggcggg acaactggaa
     4321 ctgaacgtca tgatgccggc gctgggttcg gcgctgttcg acgccatgga ctggctcacc
     4381 cacgccgtga acgccgcgac tgaaaaaggc ctgcgcggcc tcaaggtgga ccgcgaccgt
     4441 tgccgcgaac tggcgcacac cagtgtcggt ctcgccaccc tgctcaatac ctccatcggc
     4501 tacagcgctg ccgccgaggt cgccaaggaa tccgaacgca gcgggcggcc ggtgcgcgac
     4561 atcgtcgcgg aaaagggcct gatgagcacc gcggagttcg accgcctggt cgagcgcgcc
     4621 gcgatagacg gaaacataag tccacctcgc cgttaaaagc cggggatatt ccgggccgtg
     4681 caccacggga gataccggcc ccgcctgatc ttgcgtcaag accaacga
//
LOCUS       DBNGZE010000466         2995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1797970, whole genome shotgun sequence.
ACCESSION   DBNGZE010000466 DBNGZE010000000
VERSION     DBNGZE010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1797970"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /gene="dnaE"
                     /locus_tag="AC16FV_12100"
     CDS             complement(<1..1493)
                     /gene="dnaE"
                     /locus_tag="AC16FV_12100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR2850827.1"
                     /translation="MSRPFVHLRVHTEFSLADGIVRTDDLVKTAVRHGMPAVAVTDLA
                     NLFGVVKFYQAACAGGVKPIVGADVWLENPADPHKPHRLVLLCQNTEGYRRLCRLLTR
                     AYVDGQHSGKPCLSKSWFETPMAGLIALSGAQEGEIGQMLLSGNATQAEELARNYQAW
                     FPGGFYLELQRTGQPFQEDYNHAAVDLAQRLSLPVVATNHVVFLQPDDFDAHEARVCI
                     QDGRVLTDTRRPRRYTTQQYFRSSEEMADLFQDIPEAIDNTVEIARRCNLRLEFGKYY
                     LPAYPVPEGMTIDELLRQQSQQGLDARLQLMPEDKRESSRAAYSARLDLELSTITKMG
                     FPGYFLIVADFIQWAKQNGSPVGPGRGSGAGSLVAWCLGITELDPIKYDLLFERFLNP
                     ERVSLPDFDVDFCIEGRDRVIEYVNRKYGQDKVAQIITHGTMAAKAVVRDVGRVMDHP
                     YGYVDKLAKLVPFELGMTLDKALEVEPQLAERYQKEEDVRARLDTARS"
     gene            1642..2514
                     /locus_tag="AC16FV_12105"
     CDS             1642..2514
                     /locus_tag="AC16FV_12105"
                     /inference="COORDINATES: protein motif:HMM:NF022800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR2850828.1"
                     /translation="MSISITEVVTNPRVRQWLPGILNGGMLLLLTASLAQWTWLLIKP
                     PLPPLVVAPPAPAAPVNAFSLQPLLAAHLFGQVSQELTGGRLDNLPISSLNLVLTGVI
                     ASDAGGYALISVNGQPQEPFTVGQTITGGAVLQAVYSDRVVIGRNGALESLLLEGADN
                     SQPPQEMVTPAVNRAAGVSADIVRATGANQYMVARDQLAAQIRTPDFLKQATLAPSSG
                     GGFLVRQIQPNSLYEKLGLRAGDVIKSVNGQPINSAEDAIRLYQQMSSISSVQMEIIR
                     GGKSEALYYQFAQN"
     gene            2559..>2995
                     /locus_tag="AC16FV_12110"
     CDS             2559..>2995
                     /locus_tag="AC16FV_12110"
                     /inference="COORDINATES: protein motif:HMM:NF045340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850829.1"
                     /translation="MVIFRNFMTANLGGILLGALLAMPLVLQAQDQAEEANPDEPSVT
                     PVPPPAPPGSQQPGAPLTPMGVAPSPRPAAPMPPSRPSVAMPATKAPAGPALRPGEMM
                     FNFQEADIQAVVKTVSQITGKNFLLDPRVKGKLTIISTQPVSK"
BASE COUNT          627 a          908 c          876 g          584 t
ORIGIN      
        1 gagcgcgcgg tgtcgagccg cgcgcgcacg tcctcttcct tctggtagcg ctccgccagc
       61 tgcggttcga cttcgagcgc cttgtcgagc gtcatgccga gctcgaacgg gaccagcttg
      121 gcgagcttgt cgacgtaacc gtagggatga tccatgacgc gcccgacgtc gcgcaccacc
      181 gccttggccg ccatggtgcc gtgggtgatg atctgcgcca ccttgtcctg gccgtacttg
      241 cggttgacgt actcgatcac gcggtcgcgg ccttcaatgc aaaagtccac gtcgaagtcc
      301 ggcagcgaca cgcgctcggg gttgaggaag cgctcgaaca gcaggtcgta tttgatcgga
      361 tcgagttcgg tgatgccgag acaccaggcc accagcgacc cggcgccgct gccgcgcccg
      421 ggacccaccg gactgccatt ctgcttggcc cactggatga aatcggccac gatcaggaag
      481 tagccgggaa agcccatctt ggtgatggtg gacagctcca gatccagacg cgcggaataa
      541 gcggcgcggc tggattcgcg tttatcttcc ggcatcaatt gcaatctggc atccagacct
      601 tgctgcgatt gctgccgcag caattcgtcg atcgtcatgc cctccggcac cggataggcc
      661 ggcagataat attttccaaa ctccaggcgc agattgcagc gccgtgcgat ctcaaccgtg
      721 ttgtcgatcg cctcggggat atcctgaaac agatcggcca tctcctcgct gctgcgaaaa
      781 tattgctgag tcgtgtaacg gcgtggccgg cgagtgtcgg tcagtacgcg accgtcctga
      841 atacagaccc gagcctcgtg cgcatcgaag tcatccggct gcagaaacac gacatggttc
      901 gtcgccacca ctggcaacga caggcgctgg gcgagatcca cggcggcgtg attgtaatct
      961 tcctgaaacg gctggccggt acgttgcagt tccagataaa aaccgccggg aaaccacgct
     1021 tgataattac gcgccagctc ttccgcctgc gtcgcgttgc cggacagcag catctggccg
     1081 atttcgccct cctgcgcgcc cgacagggca atcagccccg ccatcggcgt ctcaaaccag
     1141 gatttgctca ggcaaggctt gccggagtgc tgaccgtcca cgtaggcgcg cgtcaacaaa
     1201 cgacagagcc ggcgatagcc ttcggtattc tggcagagca gcaccaagcg atgcggcttg
     1261 tgcgggtcag cgggattttc caaccacaca tccgcaccaa cgatgggttt tacgcccccg
     1321 gcgcatgcgg cctggtaaaa cttgaccact ccgaaaaggt tggccagatc tgtaacggct
     1381 acggccggca tcccgtgacg gacggcggtt ttcaccagat cgtccgtgcg gacgatgccg
     1441 tccgccaacg aaaattcggt atggactctg agatgaacga aaggccggct cacggccggt
     1501 atttgaccgt aaattcaggg gtcagggaag cggtataaat caacgctgcc aacgacttat
     1561 tacttaggct ttactgcatg tccagatata atgttaatta ggtacatcca gagcgctgcg
     1621 caagcaactg aaactgaata aatgagtatc agcattacag aggtcgtcac caatccccgc
     1681 gtcagacaat ggctcccggg aattctcaat ggggggatgt tgttgctgct cacggccagt
     1741 ctcgctcaat ggacatggtt gttgatcaaa ccacccctgc caccgctggt ggttgcaccg
     1801 cctgcacctg cggcgcctgt caatgctttt tcgttgcagc ctttgctggc agcgcatctg
     1861 tttggtcaag tcagccagga gctgacgggt gggcgtctgg acaatctgcc catcagcagc
     1921 ctgaatctcg ttctgaccgg ggtgatcgct tcggacgctg gcggttatgc gctgatctcg
     1981 gtcaacggcc agccgcagga gccgttcacg gtcggtcaaa caatcacggg tggcgcggta
     2041 ttgcaggcgg tgtattcgga ccgggtcgtg atcgggcgta acggcgcgct cgaaagcctg
     2101 ctgctcgagg gcgcggataa ttcgcaaccg cctcaagaga tggtcacgcc cgccgtcaac
     2161 cgcgcagctg gtgtttccgc tgacatagtg cgcgcaactg gggccaacca atatatggtg
     2221 gcgcgcgacc agctggcggc ccaaatacgc acgccggatt tcctgaaaca ggccacattg
     2281 gcaccctcca gcggtggcgg attcctggta aggcaaatcc agccgaacag tctctatgag
     2341 aagctgggtc tgcgggcggg tgacgtcatc aagtccgtca atgggcaacc gatcaactcg
     2401 gcggaagacg caatcaggct ctatcagcaa atgtccagca tcagttccgt acaaatggaa
     2461 atcatacgcg gcggaaaaag cgaagcgctt tattatcagt ttgcgcaaaa ctagtacgcc
     2521 cacacattta tcacgtcaat gaaggtattg gatcgcgcat ggtaattttc cgcaatttca
     2581 tgacagcaaa cctcggcggt attttgctgg gcgcattact ggccatgccg cttgtgttgc
     2641 aggcacagga tcaggctgag gaggccaacc cggacgaacc gtcggtgacg cccgtcccgc
     2701 cccctgctcc tcccggaagt cagcaaccgg gggcgccgct gacgccgatg ggggttgctc
     2761 cttcgccgcg accggctgca cccatgccgc catccagacc atcggtggca atgccggcaa
     2821 ccaaggcgcc cgccggaccg gcgctgcgcc ccggcgagat gatgttcaac ttccaggaag
     2881 cggacatcca ggcagtggtc aaaaccgtat cgcaaatcac cggcaagaat ttcctgctcg
     2941 acccgcgcgt caagggaaaa ctgaccatca tctcgacgca accggtttcc aagaa
//
LOCUS       DBNGZE010000467         2644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_133933, whole genome shotgun sequence.
ACCESSION   DBNGZE010000467 DBNGZE010000000
VERSION     DBNGZE010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_133933"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC16FV_12115"
     CDS             <1..822
                     /locus_tag="AC16FV_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2850830.1"
                     /translation="LATDWIRFTGGPPMNEALISGNLDFASGGVGPMVTIWARTKANL
                     KVKGICAINSMPLWLNSINPDVKTIKDFTEKDRIALPAVRVSMQAVILQMAAEKVFGK
                     GQEHKLDAWTVSLSHPDGLAQMMSGKSEITGHFTSAPFMYTELEDKRVHRVLNSYDVF
                     GGPHTFNVVWATSKLYEGEPKIVQAFLSALRIAMKEINDDPAGAAALWVKADKSKLTP
                     AQAEKIIRDKDNTWTLTPQKVMDVASYMGRVGMVPATPASWKDMFVDGIHDLPGS"
     gene            846..1643
                     /locus_tag="AC16FV_12120"
     CDS             846..1643
                     /locus_tag="AC16FV_12120"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2850831.1"
                     /translation="MPAALLEVSGVTLQYKTPRHLVTATYQVDFSIHQGDRYVILGPS
                     GCGKSTLLKAVGGFMAPVEGTIRIRGHEVDAPGPDRMMVFQEFEQLMPWKTVYQNVLF
                     PMKVTRKFARADAAARAEAAIQKVKLTKFRDVYPHMLSGGMKMRVAIARAMALEPDIL
                     LMDEPFAALDALTRRSMQQDLLALWDQLHFTVLFVTHSIEEAILVGSRILVLSPHPGR
                     VRAELDASKLDFKNIGSLEFGAMSKRIHNLLFGDESHRAGTHEEATA"
     gene            1640..2530
                     /locus_tag="AC16FV_12125"
     CDS             1640..2530
                     /locus_tag="AC16FV_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2850832.1"
                     /translation="MSAGLKDNPGAGSHRPEFEREPTDAISIGDVERPLSFVERVVNN
                     EGMQRMAILLVLVAVWELYARWLDNSLLFPTFTETLRTFWGDISNGVLIDRTLTSLRT
                     LALGYGLGLALAALLTTFAVASSIGTRILSTLTAMFNPLPAIALLPLALIWFGLGTPS
                     LVFVIVHSVLWAVALNTLSGFRSVPETLRMSGRNIGLKGARYVGLILIPAAFPSIVAG
                     LKIGWAFAWRTLVAAELVFGVSSRSGGIGWYIFEARNDLDTATVFSGLLTVILIGLFV
                     ESVIFRSLEARTVNLWGMQQ"
BASE COUNT          478 a          818 c          832 g          516 t
ORIGIN      
        1 ctcgccaccg actggatccg ctttaccggc ggcccgccga tgaatgaggc gctgatttcc
       61 ggcaatctcg atttcgcctc gggcggcgtc gggccgatgg tcaccatctg ggcgcgcacc
      121 aaggccaatc tcaaggtcaa gggcatctgc gcgatcaact cgatgccgct gtggctcaac
      181 agcatcaatc ccgacgtcaa gacgatcaag gatttcaccg agaaggaccg catcgcgttg
      241 ccggcggtgc gcgtgtcgat gcaggcggtg atcttgcaga tggctgcgga aaaagtcttc
      301 ggcaagggcc aggagcacaa gctcgatgcc tggaccgtgt cgctcagcca tcccgacggc
      361 ctggcgcaaa tgatgagcgg caagtcggaa atcaccgggc atttcacttc tgcgccgttc
      421 atgtatacgg agctcgaaga caagcgcgtg caccgcgtac tcaacagcta tgacgttttc
      481 ggcgggccgc acaccttcaa cgtcgtgtgg gcaacttcca agctctacga aggcgagccc
      541 aagatcgtgc aggctttcct gtcggcgctg cgcatcgcta tgaaggagat caacgacgat
      601 ccggccggcg ccgcggcttt atgggtgaag gccgacaagt ccaagctgac accggcgcaa
      661 gctgagaaga tcatccgcga caaggacaac acctggactc tgacgccgca gaaggtgatg
      721 gacgtcgctt cctatatggg gcgtgtcgga atggtcccgg cgacgccggc gagctggaag
      781 gacatgttcg ttgatggcat ccacgatctg ccgggcagct gacggtggac gcgagttccc
      841 gatccatgcc tgcggctcta ctcgaggtga gcggagtaac gctccagtac aaaacgccgc
      901 gccatctggt aacggcgacg taccaggttg atttctccat tcatcaaggc gaccgctatg
      961 tgatcctggg gccttcaggc tgtggcaagt ccacattgct gaaggccgtg ggcggcttca
     1021 tggcgccagt cgagggcacc atccgcatcc gcgggcacga ggtcgatgcg cccgggcccg
     1081 accgcatgat ggtgttccag gaattcgaac aattgatgcc gtggaagacc gtctatcaga
     1141 acgtgctgtt tccgatgaaa gtgacgcgga aatttgcgcg ggcggacgcg gcggcacgcg
     1201 ccgaggcggc gatccaaaaa gtgaaactca ccaagttccg cgacgtctat ccgcacatgc
     1261 tgtccggcgg catgaagatg cgcgtcgcca tcgcgcgggc gatggcgctc gagcccgaca
     1321 tattgcttat ggacgagccg ttcgccgcgc tcgatgccct cacacggcgt tcgatgcagc
     1381 aggacctgct cgcactgtgg gaccagttgc acttcacggt gctgttcgtg acccattcga
     1441 tcgaggaagc catcctcgtc ggctcgcgca tcctggtgct gtcgccgcat cccggtcgcg
     1501 tgcgggccga gctcgatgca agcaagctcg acttcaagaa tatcggcagc cttgagttcg
     1561 gcgcgatgtc gaagcggata cacaacctgc tgttcggcga cgagagccat cgtgccggca
     1621 cacacgagga agccacggcg tgagcgcggg cctgaaagac aatcccggcg cgggctccca
     1681 ccggccggag ttcgagcgcg agccgaccga tgccatttcc atcggcgacg tcgagcgtcc
     1741 gctctcgttc gtcgagcgcg tcgtcaataa tgagggaatg cagcggatgg ctatcttgct
     1801 ggttctggtc gcggtttggg agctctatgc gcgctggttg gacaactcgc tgctgttccc
     1861 gaccttcacc gagacgctgc gaacattctg gggggacatc agcaatggcg tactgataga
     1921 ccgcacctta acctcgctgc gcacgctcgc gctcggctat gggctcggtc tggcgctggc
     1981 ggcgctgctg accactttcg ccgtcgccag cagcatcggc acccgcatcc tttccacgct
     2041 gaccgctatg ttcaatccgc tgccggctat cgcgctgctg ccgctggcgt tgatctggtt
     2101 cggactcggc acgccatcac tggtgttcgt gatcgtccat tcggtgctgt gggcggtggc
     2161 gctcaacacc ctgagcggct ttcgtagcgt gccggaaact ttacgcatga gcggccgcaa
     2221 tatcggcctc aaaggcgcgc gctatgtcgg gctcattctg atcccggcgg cgttcccttc
     2281 gatcgttgcc ggcctgaaaa tcggctgggc cttcgcttgg cgcacactgg ttgccgccga
     2341 gctggtgttc ggcgtctcgt cgcgttccgg cggaatcggc tggtacatct ttgaagcgcg
     2401 caacgatctc gatactgcga cggtattctc cggcctgctc acggtcatcc tgatcggcct
     2461 gttcgtcgaa tcggtaatct tccgctccct cgaagcgcgc acggtgaatc tgtggggcat
     2521 gcagcaatga cggacggcgt gctgggagtc gatgtctggc gcggcgccga ggccggccac
     2581 ttccagccgt tcgccattcc gcgccgggcg caccagacgg tgctcgacgt ggtgaccgaa
     2641 atcc
//
LOCUS       DBNGZE010000468        10278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1454637, whole genome shotgun sequence.
ACCESSION   DBNGZE010000468 DBNGZE010000000
VERSION     DBNGZE010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1454637"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1860)
                     /locus_tag="AC16FV_12130"
     CDS             complement(37..1860)
                     /locus_tag="AC16FV_12130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR2850833.1"
                     /translation="MNNHITDDFAKPWPIRFRERLTRFGNLSLALLLAFLAARLVELP
                     GIVLTTEVPADIWTVVGRALWLDMLLFLKLLAFILPLFLLARLIFRGKRADLFGYGIV
                     GSIVLVAYLLLVNYFFVARVPLGADLFGYSLTDILGIARAGYRISLLSAASLVIPVVV
                     FWAALVYFNSHTLLPWRIVFAALLCGIFLVAFGASTLPPRTAFKTEFAYGMALNKGAF
                     FVADSYAYFTPRDSGMAVTRPVSPQFRYLDPEYPFLRDEDTPDVLGEYFRIDPATPPN
                     IVFLAIEGLGRAFSGPDAYLGSFTPFLDELAGKSLYWENFLASQGRTFASLPSILGSL
                     PFSGKGFNDLGSRMPRHLTLLSILKRNGYRTRFFCGNSLDFDNQRAFLERQNIDVMIG
                     MDDYDGSYYRLPGAYWGYADREVLQKALKTERNDPRTPYVDYVQTVSMHTPYVVPGQQ
                     EYIRLFEERMNHLGLTEASKEKYRQYRNIYSTILYTDAALRYYFEEQAKLPTYRNTIF
                     LVTGDHRLPEIPMSTKIDRYHVPLIVYSPLLKRTARIKSISSHLDIAPSLLAFLKRNY
                     RIKTPDRVAWVGSELDMEPSFRNIHRYPMKHTINNLIDYIS"
     gene            complement(1897..3258)
                     /locus_tag="AC16FV_12135"
     CDS             complement(1897..3258)
                     /locus_tag="AC16FV_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HIR2850834.1"
                     /translation="MKERTRLLVAVWVLLVGIVAGNPAWAADAATAMAAGNAARDRGD
                     YAAAIEHYRAAVAAAPKSYEAKFQLARTLSYTNQRQEAIRLYTELLVTRPNNSDLLLA
                     RGRTYAWEGRWSEAEADLTAVTTRLPGYGDAWSALGDMYLWSDRPDFAVKAYGRWIAA
                     QPTEPRAYLARARAYRAVGDLDAARADLGAARAHGAAVAEVDRLLAALERRAEPEAAT
                     PEGFLWAASLNYSFSHFSPDRDKWNEYTASVRHYWQHASLAVEYLKANRFALTDDAVA
                     LDAYIDLWSRAYANVRYQYSPDAALYPEDAYRAEVFQGVGQGWELSGSYDRLNFTNTN
                     VNMYGVGLGKYTGNWYLRWRTLFIPSTAKSGTSHRAIARYYFSGDADDYVEVNGGFSR
                     GGDFRGQSQIIDVTSGWTVGAAVRKYFSSRWGVRLAADYSDEQTANPFVERSVSASLL
                     FRW"
     gene            complement(3364..4869)
                     /locus_tag="AC16FV_12140"
     CDS             complement(3364..4869)
                     /locus_tag="AC16FV_12140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2850835.1"
                     /translation="MTEYFPIVESFILLYFIGLNGVYLMLNVLSLSGIARYMQSRDVA
                     GLPHLFSGFAPPVTIVVPAYNEEAHILDTLRSLFQHNYPEYEIVVVNDGSKDRTLDIM
                     KKEYSLAPFPEAYRARIRTKPVRAVYHSTVDPRLRVVDKENGGRGDAVNAGINIARYP
                     WVCRVDADSILQKDSLMLIVQPLLEDPTVVACGGTVRVANGCKSKDGIFIEADLSRKP
                     LVLFQIVEYLRAFLYGRMGWSPMNAHLIISGAFGIYHKETLVSVGGYRHDTIGEDMEL
                     IVRLHRILSEQRKRYRITFVPEPICWAEVPDDLKRLKKQRIRWQIGLVESLMMNRGLL
                     FHDKGTFAGWLAFPFALFFEWFGPFVEILGYIIVIAGFIYGFISIQAFITFLTLAVGF
                     GVLLSTSALLLEEMSFHLYKKPRHILALFAASLVENFGYRQLNTVWRIIAVYYYLRDK
                     IREKLAARKQKGRPRPRLKTPETQMNADNIKEPLIYSPVHPEPVEGRMKSD"
     gene            complement(4872..5930)
                     /locus_tag="AC16FV_12145"
     CDS             complement(4872..5930)
                     /locus_tag="AC16FV_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2850836.1"
                     /translation="MRSTLVLTVAYWVSVAVVALVMFLMTYIFILRTLLLLKERRYRD
                     LSATWRRILLSSEEANRDRLPCIKYVDRTSFLILWNMLREEKDGDAKTRDWMNQVAVA
                     MGMDRVARHLLTKRNIRKKLLAVVTLGQLRDKSQWDKLRQIATSDHALLSLAATQAIA
                     RIDPVAAVPIVIPLAVRRTGWPAAKVASILKEIGPDLISKPLTDAVLEAPDEEMQRLI
                     PFLETCHDTAALSTAHRILREPHDDKVIGPCLYVLGKFRDKKDLKLIRQYLTYPRWHV
                     RAHAAACLGRIGTKEDEAKLAALLADPEWWVRYRAAQAIVNLPFSNTARVHKLKETLS
                     DRYARDILTQAIAEKGLA"
     gene            complement(6178..7455)
                     /gene="serS"
                     /locus_tag="AC16FV_12150"
     CDS             complement(6178..7455)
                     /gene="serS"
                     /locus_tag="AC16FV_12150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR2850837.1"
                     /translation="MLDPKLLRSNLDEVARQLGRRGYRLDTQALTTLEEKRKKVQVIA
                     QQFQSERNAKSKAIGVAKSKGEDTSRLMQEVANIGAALKQSETELEQIQNDINEIALG
                     IPNIPHASVPDGKDESDNQEIRRQGEPRRFDFTPKDHVDLGAALSMMDFDAATKIAGA
                     RFVVMSGALARMHRALIQFMLDLHTREHGYTETYVPYMVNADSLRGTGQLPKFEQDLF
                     ALGGEQRYYLIPTAEVPVTNIVRDVIVDAGDMPRKYVCHTPCFRSEAGSYGKDTRGMI
                     RQHQFEKVEMVQVVRPQDSYEALEELTGHAETVLQRLELPYRVVTLCTADMGFSAAKT
                     YDIEVWLPGQQKYREISSCSNFEDFQARRMQARWRNPETGKPELVHTLNGSGLAVGRT
                     LVAVMENYQDSHGNIDIPTVLRPYMQGETRICR"
     gene            complement(7497..7805)
                     /locus_tag="AC16FV_12155"
     CDS             complement(7497..7805)
                     /locus_tag="AC16FV_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HIR2850838.1"
                     /translation="MTVSEITMVRIYLTEAGELQKTLLAKLHDEEKVRGVTVFRGVSG
                     FGRSGKVHSSTLLDLSLDLPVVIEFFDTPEKVKRILTHLRKILPPGHVVSWSAHVNEG
                     "
     gene            complement(7802..8176)
                     /gene="crcB"
                     /locus_tag="AC16FV_12160"
     CDS             complement(7802..8176)
                     /gene="crcB"
                     /locus_tag="AC16FV_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR2850839.1"
                     /translation="MRQVLAIAAGGAVGSLLRFWMSNWVHSFADRSFPYGTLVVNVLG
                     CLIMGFLFVLFIDRLSDNLVLRAGILIGVLGGFTTFSSFSIETFNLIEQGAWVRAVAN
                     MSGSLVLCVGATWIGVILGRQI"
     gene            complement(8217..8723)
                     /locus_tag="AC16FV_12165"
     CDS             complement(8217..8723)
                     /locus_tag="AC16FV_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2850840.1"
                     /translation="MNRTNLIIAGVVLVLLSLPVAADKPAPVAVDRFQSGASAFDAGQ
                     YAKARELWLPLAENGDVRAQHALGRMYEKGKGVERDFAQAFIWYRKAAEKDNADSQYR
                     LAVGYAYGLGVKRDEATALSWLRKAANNGHKRAQKTLARAYEEGRFGLTADQGQAKYW
                     YDKANSGS"
     gene            complement(8746..10125)
                     /locus_tag="AC16FV_12170"
     CDS             complement(8746..10125)
                     /locus_tag="AC16FV_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR2850841.1"
                     /translation="MATQDLFPETQSMPWQPLADRMRPQRLEDFFGQSHLLTPGKPLR
                     QAIEAGILHSMIFWGPPGTGKTTLARLIARRTQAHFIAISAVFAGVKEIHAAVEEAKQ
                     ARAEGHATVLFVDEVHRFNKAQQDGFLPYVEDGTLTFIGATTENPSFELNNALLSRAR
                     VYVLKALTADEIRLAIDRALTDEVAGLGAQHLEMPPEARELLAQASDGDARRALNLLE
                     MASDLATKLHDKPTITTDLIKEVVTGGVRRFDKQGEAFYDQISALHKSVRGSDPDASL
                     YWFTRMLDGGCDPLYIARRVVRMASEDIGNADPRALQLALNAWDVQERLGSPEGELAI
                     AQAVVYMACAPKSNAVYTAMGAAMDDAKKRGSLEVPLHLRNAPTKLMKELGYAKGYRY
                     AHDETEAYAAGESYFPEELGKQQYYFPTEQGLEGKIAERLAHFRELDKKAREAGARQE
                     KRKPGTNAK"
BASE COUNT         2300 a         2985 c         2834 g         2159 t
ORIGIN      
        1 gatggctaac aggttgctgt catttaagaa atacattcaa gatatgtaat ctatcagatt
       61 attgatcgtg tgcttcatgg ggtagcgatg gatgttccgg aacgagggct ccatgtccag
      121 ctccgagccg acccaagcga ccctatcggg ggtcttgatc cggtagttgc gcttcaggaa
      181 tgccagcagc gagggggcaa tgtccagatg cgaggagatg gacttgatcc gcgcggtgcg
      241 tttcagcagc ggcgaataga cgatcagggg cacgtgatag cggtcgatct tcgtcgacat
      301 gggaatctcc ggcagccgat gatcgccggt gacaaggaaa atggtgttcc gataagttgg
      361 cagcttcgct tgttcttcga agtaatagcg cagcgccgca tcagtataga ggatggtcga
      421 atatatattc cggtactggc gatatttttc cttggaggct tccgtcaaac caagatgatt
      481 catacgttct tcaaaaagcc gaatatattc ctgctgtccg ggaacgacat acggggtatg
      541 catgctgact gtctgcacat aatccacata cggtgtccgc ggatcatttc gttctgtttt
      601 caaggctttt tgcaacactt ccctgtcggc atagccccaa taggcaccag gaagccggta
      661 ataactgccg tcatagtcat ccatgccaat catcacatcg atattctggc gctcaaggaa
      721 ggcacgctgg ttatcaaaat ccagactgtt cccgcagaag aaacgagttc gataaccgtt
      781 gcgcttcagg atgctcagca atgtcagatg tcttggcatc cgtgaaccca agtcgttgaa
      841 acccttgcca ctgaacggca gcgatcccag gatcgagggc aacgaagcga aggtccgtcc
      901 ctgggaggca aggaaatttt cccagtacag gcttttgccg gccagttcgt cgagaaacgg
      961 ggtgaaactg cccagatagg catctggacc gctgaaggcc cgccctagcc cctcgatcgc
     1021 gagaaacaca atgtttggcg gcgtcgccgg atcgatcctg aaatattcgc ccaagacgtc
     1081 aggcgtgtcc tcgtcccgca gaaacggata ctccggatcg agataacgaa actgcgggct
     1141 gaccggccgg gtgacggcca tgcccgaatc gcgtggcgtg aaataggcat aggagtcagc
     1201 aacaaaaaag gcccctttgt tcaaggccat gccataggca aactcagtct tgaatgccgt
     1261 ccggggaggc aaggtggagg caccaaaagc caccaggaaa atcccgcaca gcagcgcggc
     1321 aaaaacaatt cgccagggga gtagcgtgtg actgttgaaa tataccagcg ccgcccaaaa
     1381 aacaacaact ggaatgacca gactggccgc ggacaaaagg gaaatccggt aaccggcacg
     1441 tgcgatgccg aggatatcgg tgagagaata gccgaacaaa tcggccccca gcggaactcg
     1501 tgccacaaag aagtaattaa caagaagcag ataggcaacg agaactatcg agccgacgat
     1561 accatagccg aatagatcgg cgcgtttccc gcgaaaaatc aggcgggcca acagaaacaa
     1621 aggcaggatg aacgcaagca gtttcagaaa gagcaacatg tccagccaca acgccctgcc
     1681 aaccacagtc cagatatcag caggaacctc tgtggtcaga acaatgccag gcaattccac
     1741 caatcgcgcc gccagaaagg cgagcaataa tgccagcgac aggttaccga atctggtcaa
     1801 tctttcacga aaccgtatcg gccagggttt ggcaaagtca tctgtgatgt gattgttcat
     1861 tccttacatg aacagcgaaa ccgccatgcc ccattatcac cagcggaata gcaagctggc
     1921 cgaaacgctt cgctcgacaa agggattggc agtttgttca tcgctgtagt cagctgcgag
     1981 cctgacgccc cagcgtgaag agaaatactt tcggaccgcc gcgccaaccg tccagccgct
     2041 ggtgacgtcg ataatctggc tttgtccgcg aaagtccccg ccgcggctga atccgccgtt
     2101 cacctcgaca taatcatccg catctccgga aaaataataa cgggcaatcg cgcgatgcga
     2161 ggtgcctgac ttggcggtcg atggaatgaa aagcgtgcgc cagcgcaaat accagttacc
     2221 ggtgtattta ccgagcccga ccccgtacat gttgacattg gtattggtga aattcaggcg
     2281 gtcataactg cctgagagct cccatccctg ccctaccccc tggaagacct ccgcacgata
     2341 cgcgtcctcg ggataaagcg cggcatcagg cgagtactga tagcgcacat tggcatacgc
     2401 ccgggaccag agatcaatgt aggcgtcgag cgcgacggca tcgtcggtca gcgcaaaacg
     2461 attcgccttg agatactcga ccgcgagcga ggcgtgctgc caatagtggc gcacggaggc
     2521 cgtgtactcg ttccatttgt cccggtccgg cgagaaatgg ctgaaactgt aattcaggct
     2581 tgcggcccac aggaaacctt ccggtgtggc cgcctccggc tccgctcgcc gctccaatgc
     2641 cgccagaagc cggtcgacct ccgcgactgc ggcgccgtgt gcgcgtgccg cccccagatc
     2701 tgctcgtgcg gcatcgaggt cgcccaccgc gcggtaagcc ctggctcggg ccaggtacgc
     2761 cctgggctcg gtgggttgcg ccgcaatcca tcggccgtag gctttgacgg cgaaatccgg
     2821 gcggtcgctc cagagataca tgtctccgag cgccgaccag gcatcgccat agcctggcaa
     2881 gcgggtggta actgcggtca ggtcggcttc ggcctcggac cagcgccctt cccaggcata
     2941 agtgcgcccc ctggcgagca gcagatcgga attattcggc cgcgtgacca gcaactcggt
     3001 atagagccgt atcgcctcct gacgctggtt cgtatacgag agcgtgcgcg ccagctgaaa
     3061 ctttgcctcg tacgatttcg gcgccgccgc cacggccgcg cgatagtgtt cgatcgccgc
     3121 tgcataatcg ccccgatccc gcgccgcgtt accggctgcc attgccgtcg ccgcgtccgc
     3181 cgcccacgcg gggttacctg ccacgatccc tacgagcaga acccacaccg ctacgagcag
     3241 ccgcgttctt tctttcacta tttttatccc aagaataatc cgcgtttatc tctttaggaa
     3301 cttctgatta attccgttct tggtgagctt gtcgaaccac gaacggacag aaacgtaatc
     3361 cgttcaatcg cttttcattc gcccttcgac aggctcaggg tgaacgggcg aataaatcag
     3421 aggttccttt atattatctg cgttcatctg cgtttcaggt gtttttaacc gaggtcgcgg
     3481 ccggcccttt tgtttccgtg cggccagctt ctcgcgtatc ttgtcacgca ggtagtagta
     3541 aaccgcgatt atcctccata cggtgttcag ctgccggtat ccaaaatttt cgaccaagga
     3601 cgctgcaaac agcgcaagga tatgcctggg ttttttatac agatgaaacg acatttcctc
     3661 cagaagcagc gccgaggtgg acagcagcac gccgaatccc accgccaagg tcaaaaaggt
     3721 tatgaatgcc tgaattgaaa taaatccata gataaaaccg gcgatgacga taatgtaacc
     3781 gagaatctcg acaaacgggc cgaaccattc aaagaaaagc gcgaagggga aagcgagcca
     3841 tccggcaaac gtccccttgt catgaaacaa caggccacga ttcatcatga gcgactcgac
     3901 caggccgatt tgccagcgga tacgctgttt tttcagccgt ttaaggtcat cgggcacctc
     3961 cgcccagcag atgggttcag gcacgaatgt gatgcgatag cgtttccgct gctcggacaa
     4021 gatgcgatga agccggacaa tcagctccat gtcttcgcca atggtgtcat gccggtagcc
     4081 gcccaccgac accagcgttt ccttgtgata gatgccgaag gcgccggaaa tgatcagatg
     4141 ggcgttcatg ggcgaccacc ccattcgccc gtacagaaag gcccgcaaat attccacgat
     4201 ctggaacaat acgagcggtt tcctggaaag atcggcctcg ataaaaatcc cgtccttcga
     4261 tttgcacccg ttggcgacgc gaacggtccc gccgcaggcc acgacggtcg ggtcctcgag
     4321 aagcggttgc acgatcagca tcaggctgtc cttctgcagg atggaatcgg catccacgcg
     4381 acaaacccac gggtaacgcg cgatattgat gccggcgtta acggcgtcac ccctgccgcc
     4441 gttttcctta tcgacgacgc gcagacgcgg gtcaacggtg gagtgataga cggcgcgcac
     4501 gggttttgta cgaatgcggg cgcgatatgc ctccggaaac ggggccaggg agtattcttt
     4561 cttcatgata tcgagggtgc ggtccttcga tccgtcattc accacaacta tctcgtattc
     4621 agggtagttg tgctggaaca gcgaacgcaa cgtatccaga atatgggcct cttcgttata
     4681 agcaggaacg acgatcgtaa cgggcggagc gaacccggaa aataaatgcg gcaggccggc
     4741 gacatcgcgg ctttgcatgt agcgcgcgat gccgctgagc gagagaacat tcagcatcag
     4801 atagaccccg ttgagcccga taaaatagag caggataaac gactcaacga tggggaaata
     4861 ttcggtcatc gtcaggccaa ccccttttcg gcgatggcct gcgtcagaat atccctggca
     4921 taacgatcgc tcagcgtttc cttgagctta tggactcgcg cggtattgga aaaaggcaga
     4981 ttcacgatgg cctgcgcggc gcggtatcgc acccaccact ctgggtcggc aagcaatgcc
     5041 gccagttttg cttcatcttc tttcgtccca atccttccga ggcaggcggc ggcatgggcg
     5101 cgcacgtgcc agcgaggata cgtcagatac tggcgtataa gtttcaaatc cttcttatca
     5161 cggaattttc caagcacgta caaacaaggg ccgatcacct tgtcgtcgtg aggctccctt
     5221 agaatccggt gcgcggtgga cagagcagcg gtgtcatggc aggtttcaag aaatggaatc
     5281 agcctttgca tttcttcatc cggcgcttcc aggacggcgt ccgtcagagg tttcgatata
     5341 agatcgggac cgatctcctt gaggatactg gcgaccttcg ccgccggcca tcctgtacgg
     5401 cgcacggcca gcggaataac gatcgggacc gctgccacgg ggtcgatgcg cgctatcgcc
     5461 tgtgtcgcgg ccaacgacaa gagcgcatgg tcggaagtcg ctatctggcg caatttgtcc
     5521 cactggcttt tatcgcgcag ttgcccaagc gtcacgaccg caagaagttt tttacgaata
     5581 ttgcgttttg tcagcaaatg tcttgcaacg cggtccatcc ccatcgcgac ggcgacctga
     5641 ttcatccaat cccgggtttt cgcatcgccg tccttttcct cgcgcaacat gttccacagg
     5701 atcaggaacg aagttctgtc cacatattta atgcatggca ggcggtcgcg attggcctcc
     5761 tcgctactca gcagaatccg gcgccacgtc gctgaaagat cgcggtatcg ccgttccttc
     5821 agaagcaaca aggtgcgcag tatgaaaatg taggtcatca gaaacatcac cagcgccaca
     5881 actgcgacgg agacccagta ggctacggta agaaccagag tggaacgcat gtcaggaacc
     5941 gacgcttacc ctgttccctg tgcggttggc gcgcggcaac cggcggcgtc gcgtttttca
     6001 accccggcag cgaaggaaat gacaatgaga aagcctgtca taaaaaaaca gacaacttgg
     6061 ttgttgtgct catgaacccc accagcatga tccaccgctc agttcagcgt ctcggcaccc
     6121 gctccggcgc acactgcatt atttttccaa catattatca gcaaatacta tgccagatca
     6181 gcggcaaata cgtgtttctc cctgcatata gggacgcaga acagtgggga tgtcgatatt
     6241 tccgtgggaa tcctgataat tttccatgac cgctacgagc gtgcggccca cggcaaggcc
     6301 cgagccgttg agcgtgtgca ccagttccgg cttgccggtc tctgggttgc gccagcgcgc
     6361 ttgcatgcgc cgcgcctgga aatcctcgaa gttgctgcac gaggatatct cgcggtattt
     6421 ctgctggccc ggcaaccata cctcgatgtc gtaggtcttg gcggctgaaa aacccatgtc
     6481 ggcggtgcac agcgtgacga cccgatacgg cagttcgaga cgctgcaaga cggtttccgc
     6541 gtgaccggtc agttcttcca acgcttcata tgaatcctgc ggccgcacta cctgaaccat
     6601 ttccaccttt tcgaactgat gctggcgaat catgccacgt gtgtctttcc cgtatgaacc
     6661 tgcctcactg cgaaaacacg gcgtgtgaca gacgtatttc cggggcatgt caccggcatc
     6721 aacgatcacg tcacggacga tattggtcac ggggacttcc gccgtgggga tcaggtaata
     6781 gcgctgttcg ccgcccagtg cgaacaggtc ctgctcgaac ttcggcaatt ggccggtgcc
     6841 gcgcaggctg tcggcattga ccatataagg aacataggtc tcggtgtaac cgtgttcgcg
     6901 cgtatgcagg tcgagcatga actggatgag cgcgcgatgc atgcgtgcca gagcgccgct
     6961 catgacgaca aaccgtgcac cggcgatctt ggtcgccgca tcaaaatcca tcatgctcag
     7021 ggccgcgccc agatcgacgt gatccttcgg cgtgaaatcg aatctgcgcg gttccccctg
     7081 gcgccggatt tcctggttgt cgctttcgtc tttaccgtcc ggcacgctgg cgtggggaat
     7141 attggggatg ccgagcgcga tttcgttgat gtcgttctga atctgctcaa gttcggtttc
     7201 cgactgcttg agcgcggcgc cgatattggc cacctcctgc atgagccgag aagtatcttc
     7261 gcccttcgac ttggccacgc cgatcgcctt ggatttcgcg ttgcgctcgc tctggaactg
     7321 ctgggcgatc acctgcacct ttttgcgctt ttcttcaagc gtggtcagcg cctgcgtgtc
     7381 cagccggtaa ccgcggcggc caagctggcg cgcgacttcg tcgaggttgc tacggagcaa
     7441 tttggggtcg agcatgtttg tatttattta ttaaggtcta tgccttggat cgcgaatcag
     7501 ccttcgttga cgtgtgcgct ccaagaaacc acgtgtccgg gcggcagtat ttttctgaga
     7561 tgtgtcagaa tgcgcttcac cttttccggt gtgtcgaaaa attcgataac cacgggcaga
     7621 tcaagcgaga gatccagcaa agtcgaggaa tgtaccttgc cagagcgtcc gaaaccggaa
     7681 acgccgcgga acaccgtcac gccgcgcacc ttttcctcgt cgtgcagttt ggccagcagg
     7741 gttttctgca gctcgccggc ttccgtcaga tagatacgca ccatggtgat ttcgctgact
     7801 gtcatatctg tctccctagt atcacaccga tccaggtggc gccgacgcac agcaccaggc
     7861 ttccactcat attcgccacc gccctgaccc aagcgccctg ctcgatcaga ttgaaggttt
     7921 cgatggaaaa cgacgagaag gtggtgaagc cgcccagaac acctatgagg attccggcgc
     7981 gaagcaccag gttgtcgctc agtcggtcga tgaaaagcac gaacagaaaa cccatgatca
     8041 ggcatcccag cacgttcacc accagggtgc cgtagggaaa cgagcggtcc gcgaacgaat
     8101 gcacccaatt ggacatccag aatcgcaaca ggctacccac ggctccaccc gcggctatcg
     8161 ccagcacctg cctcatgggg atgtgcgcct cactggtgga atgatctaga tttttctcat
     8221 gaaccggaat tggccttatc gtaccaatac ttcgcctgtc cctggtcagc cgtcagacca
     8281 aaacggccct cctcataggc gcgcgccagc gttttctgcg cgcgcttgtg gccattattc
     8341 gccgccttgc gcagccagga tagcgccgtg gcttcatcac gtttcacgcc caaaccataa
     8401 gcgtaaccga cagcaaggcg gtactggctg tcggcattat ccttttccgc cgcctttcga
     8461 taccagatga atgcctgcgc aaaatcgcgc tccacgccct tccctttttc gtacatccgg
     8521 ccaagcgcgt gttgcgcgcg gacgtcgccg ttttcagcga gaggcagcca caattcacgc
     8581 gccttggcat actggccggc atcaaacgca gacgcaccgc tctggaacct gtcaaccgca
     8641 acaggagcgg gtttatccgc cgccaccggc agcgagagca gaacgaggac gacgccggcg
     8701 ataatcagat ttgtgcggtt cattgtttct cgctcgtgaa aagatttact tggcattcgt
     8761 cccgggtttt cgtttttcct gtctggcacc ggcctcccgc gcttttttat cgagctcacg
     8821 aaaatgggcc agacgctcgg caattttacc ctccaatccc tgctcggtgg gaaaataata
     8881 ctgctgcttt cccagctcct ctggaaaata gctttcgccg gcagcgtagg cctcggtctc
     8941 gtcatgcgca tagcgataac ccttggcgta acccagttcc ttcatgagct tggtcggggc
     9001 attacgtaaa tgcagcggca cctccagcga accgcgcttt ttggcgtcgt ccatggcggc
     9061 gcccatggct gtgtacacgg cattgctttt tggcgcgcag gccatgtaaa cgaccgcctg
     9121 ggcaatggca agttcacctt cgggactgcc caagcgctcc tgcacgtccc aggcattcaa
     9181 ggccaattgc aaggcacgcg gatcggcatt gccgatgtcc tccgaagcca tgcgcaccac
     9241 acggcgggcg atgtatagag gatcacagcc gccatcgagc atgcgcgtga accaatataa
     9301 agaagcgtcc ggatcggagc cacgcaccga tttatgcaac gcggatatct gatcgtagaa
     9361 cgcctcgccc tgcttgtcga aacggcgcac cccacccgtg actacttcct ttatgagatc
     9421 agttgtgatg gtcggcttgt catgtaactt cgtggcgaga tcggacgcca tctcgagcag
     9481 gttcaatgcc cggcgcgcgt cgccatcgga cgcttgcgcc agcagctcgc gcgcctcggg
     9541 cggcatttcc agatgctgcg cgcccagacc ggcgacttca tccgtgagcg cgcgatcgat
     9601 ggccagacgg atctcgtctg cggtcagagc cttgagcaca tagacacgtg cgcgcgacaa
     9661 caaggcgttg ttcagttcaa atgaggggtt ttccgtggtg gcgccgatga aggtcaacgt
     9721 gccgtcctcc acgtaaggca ggaatccgtc ttgttgcgct ttattgaagc gatgcacctc
     9781 gtccacgaac aggacggtgg catgaccctc cgcgcgagct tgtttggctt cttccacggc
     9841 cgcatggatt tccttgacgc cggcaaaaac cgcggagatg gcgatgaaat gcgcctgagt
     9901 gcgccgcgcg atcagacgcg ccagcgtggt cttgccggtg ccgggcggtc cccagaaaat
     9961 catggagtgc aggatgccgg cctcgatggc ctgccgcagc ggcttgcccg gcgtgagcag
    10021 atgcgattga ccgaaaaaat cctccagccg ctgtggacgc atgcgatccg ccagcggttg
    10081 ccacggcatg gattgggttt ccggaaacag gtcctgcgtc gccatacgat gaatatatga
    10141 tttatatgat catctgtcgc aaagacgcga agacgcaaag aaaagatatt gagaataaaa
    10201 cccggaaaac ctttgcgcct ttgcgtcaaa aactcataaa tcaggggttt cttgagatcg
    10261 gaacgctcat tcgccgac
//
LOCUS       DBNGZE010000469         6533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_64815, whole genome shotgun sequence.
ACCESSION   DBNGZE010000469 DBNGZE010000000
VERSION     DBNGZE010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_64815"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC16FV_12175"
     CDS             <1..261
                     /locus_tag="AC16FV_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HIR2850842.1"
                     /translation="SGQYGFVATEMSWPITHMVAPKDDALECRQCHSAASRLHKIEGI
                     YMPGRDSNKYLDMLGGGAAFFALLGVIGHGALRIFSNRKGGK"
     gene            263..868
                     /locus_tag="AC16FV_12180"
     CDS             263..868
                     /locus_tag="AC16FV_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR2850843.1"
                     /translation="MAVERIYIFKRFERFWHWAQAATIIFLMLTGFEVHGVYKFFGFA
                     KAVNLHTIAAWTLITLWVFAVFWHFTTGEWRQYIPTLEKVDAMFKYYLTGIFTHAPHP
                     FRQTTLRKHNPLQRLAYLFFWLVISPVIWFSGWFYLFYGQWNDWGVDRFLSLQWVAFF
                     HVLGAYMVLVFFIAHIYLATAGHTPTSHIKAMITGWEEVED"
     gene            complement(1028..1933)
                     /locus_tag="AC16FV_12185"
     CDS             complement(1028..1933)
                     /locus_tag="AC16FV_12185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2850844.1"
                     /translation="MKWVNFLRSLITIESRRMRILSMVLVMILSVASAPTPATERDAT
                     DANAVPHLNDRGRREYREYLKAEMHRAFVIAPGGAWAWKAGMDTPEEALENALAECNT
                     EAEQTCIPYALNDNVVLDEKKWLTLWGPYLTEEQAATVPEGTHRGELFPDLVLTDPKG
                     RPFKVSELRGRIAVLHFWGTWCPSCVHELPQFVKLRQALSNTPDVVFVFSQVREPLAV
                     ARQWLIQQNLNLPLYDSGSHGASEDRLRLSNGRLIADRALAPAFPTTYVLDRYGTVVF
                     SMRGSATDWSQYAAFLRDAAARSGK"
     gene            2073..2690
                     /locus_tag="AC16FV_12190"
     CDS             2073..2690
                     /locus_tag="AC16FV_12190"
                     /inference="COORDINATES: protein motif:HMM:NF020254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HIR2850845.1"
                     /translation="MTTFFAIGIVLNVVLTGLALYWLWCQRIPKQGKDETGESRNDHG
                     QNRHERSSAPDASNNYLAEYVRLLTGSYRHWTGRDLVDPGLSAVEQARALYEAPFVVA
                     SHDAGNDPVFNYANCAALALFETGWRDFTSMPSRLSAEPMEREQRAQLLERVGRDGFI
                     NDYSGIRISTTGRRFRIRNATVWNLVDAYGVPMGQAVMFRDWEYL"
     gene            2756..3388
                     /locus_tag="AC16FV_12195"
     CDS             2756..3388
                     /locus_tag="AC16FV_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR2850846.1"
                     /translation="MTTQRILWYFADPMCSWCWGFAPVIGAIRETYSERIKIVLMLGG
                     LRPGTTEPMTPKAREEILQHWRDVHRMTGQPFVFEGAMPEDFVYDTEPPSRAVIAVAD
                     IKPEAVFPYFKSVQEAFYAHGRNVTQPDTLADLAEQHNIGRAMFLDRFDSDDIRKKTQ
                     THFQIARQSGVRGFPTVILQSGTTGTLLTHGFRPFTELQPDIDQWLARME"
     gene            3880..5016
                     /locus_tag="AC16FV_12200"
     CDS             3880..5016
                     /locus_tag="AC16FV_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2850847.1"
                     /translation="MKIIAVANQKGGCGKTTTAINLAACLGKREQRVLLLDLDPQGHS
                     SLGFGVFNEDARDLYDVFTGEIRLEEIILPNVFTGVDVVPATKTLAAAEHLPVRSDER
                     DKQLEKHLASIRDRYDYLVIDCPPSMGLLCLNGLAVSDLVLIPIEMSLFGMHGIDRMY
                     EVIRELRERHGREIPVRVLPTLVDSRTRLCRQFLHEIGERFADDVLSVMVQFTVRLKE
                     AVRQGAPIIAYDPTSTVAVQYDRLANEMMSSLQENVATDSEGEVKTRLNAMKRAFEEA
                     RSRLAGHAGRQKVVLRFYDFAGRDVKLAGSFNDWRPDQGVVTRTEKGIVEKIVMLMPG
                     TYQYRLIVDGIWQEDPSNPEQVSNYSGGFNSVLRVEEEHEAEHA"
     gene            5165..6109
                     /locus_tag="AC16FV_12205"
     CDS             5165..6109
                     /locus_tag="AC16FV_12205"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HIR2850848.1"
                     /translation="MTDLSALSVRFGLSGQISFKEIPGGLTALEVTNTHGSATIALQG
                     GHVMRWQPKSEAEPVVWLSRFAKFAPGKSIRGGVPLCWPWFGPHATDPKLPAHGFART
                     VPWEVTATRELNDGATEIALALVETDQTRAMWPHRTELSINITVGPTLKIALTTDNPG
                     NEDVVIGEALHTYFQIGDIADIRIEGLDGCDYLDKVGGGSVRRHQDGPVVFSAETDRI
                     YLNTEAKCVIEDARLKRRIHVAKSGSRSTVVWNPWTEKADKMGDFGPEGWRRMVCIES
                     ANAAENVVAVRAGGRHTLAVEYWAEKFPGQSQRGPYSE"
     gene            6196..6489
                     /locus_tag="AC16FV_12210"
     CDS             6196..6489
                     /locus_tag="AC16FV_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR2850849.1"
                     /translation="MEIPQRDGDGYLIDRDKWTPEIARAMAEVDGMALDEVKWSHIIK
                     ARDYYEEFGVVPPIRKFAKFIEEDQKDLFKLWMTGPMKPITKYGGLPKPTGCV"
BASE COUNT         1368 a         1840 c         1905 g         1420 t
ORIGIN      
        1 agcggccagt acggtttcgt cgcgaccgag atgtcctggc cgatcacgca catggttgcg
       61 cccaaggacg atgcgctcga gtgtcgccag tgccacagcg ccgcgagccg cctgcacaag
      121 atcgagggca tctacatgcc gggacgcgat tccaacaagt acctcgacat gctcggcggc
      181 ggcgcggcgt tcttcgcgct gctcggggtc atcggtcatg gcgccttgcg tatcttcagc
      241 aaccggaagg gagggaaata acatggccgt cgaaagaatc tacatcttca aacgcttcga
      301 gcggttctgg cactgggcgc aggcggcaac gatcatattc cttatgctga ccggcttcga
      361 ggtgcacggc gtgtacaaat tcttcggctt cgccaaggcg gtgaacctgc ataccatcgc
      421 cgcctggacg ctgatcacgc tgtgggtgtt cgccgtgttc tggcatttca ccaccggcga
      481 atggcgtcag tacatcccga ccctggagaa ggtcgatgcc atgttcaagt attacctcac
      541 cggcatcttc acccacgcgc cgcatccgtt ccgccagacc acgctgcgca agcacaaccc
      601 gttgcagcgt ttggcctatt tgtttttctg gctggtaatc tcgccggtga tctggttctc
      661 cggctggttc tatctgttct acggccagtg gaacgactgg ggcgtggacc ggttcctgtc
      721 attgcagtgg gtggcgttct tccacgtgct gggcgcgtac atggttctgg tgttcttcat
      781 cgcccacatt tatctcgcca ccgccggtca cacgccaacc tcccacatca aggccatgat
      841 caccggctgg gaggaagtgg aagactagaa tctatctgat gaacccctaa caaaaagaaa
      901 tttaaccgcc aagacgccaa gaacgccaag tttttactgt taaaaaaatg gtctttcttg
      961 gcgtcttggc gacttggcgg ttcaaagtca tatttatttt tcccccgtgt tccccgtggt
     1021 ttagttttta ttttccggag cgcgccgcgg cgtcgcgcag gaacgcggcg tactgtgacc
     1081 agtccgtcgc cgaaccgcgc atcgagaaca ccacggttcc gtaacgatcc agtacataag
     1141 tggtagggaa agccggggcg agcgcacgat ccgcgatcag acgaccgttg cttagccgta
     1201 gccggtcctc gctggcgcca tgcgagccgg aatcatacag cggcagattc aaattttgct
     1261 gtatcagcca ttggcgcgcg acggccagcg gctcgcgcac ctgactgaac acgaacacga
     1321 catccggcgt attgctcagc gcctggcgca gcttcacgaa ctggggcaat tcatggacgc
     1381 aggaagggca ccacgttccc cagaaatgca gcacggcaat tctgccccgc agttcggaaa
     1441 ccttgaacgg ccggcccttt gggtcggtca gcaccagatc cggaaaaagc tcgccccggt
     1501 gcgtgccctc tggcacggtc gcggcctgtt cttcagtcaa atacggaccc cacagggtca
     1561 gccatttctt ctcatcgagc actacattgt cgttaagtgc gtagggtatg caggtttgct
     1621 cggcttcggt attgcattcc gccagcgcgt tctcgagcgc ctcctcaggc gtatccatgc
     1681 cagccttcca ggcccaggcc ccgccggggg caatgacaaa cgcccggtgc atctccgcct
     1741 tcaggtattc gcggtactcg cgacggccgc gatcgttgag atgcggaacc gcgttcgcgt
     1801 ccgtagcatc ccgttccgtt gccggggttg gagcactggc aacagacagg atcatgacaa
     1861 ggaccatcga cagtatccgc atccggcggg attcgatggt gatgagcgat cgcaaaaaat
     1921 tcacccattt catgggacgc gattgtacct gatgttggca ggctcgtcgg gtgccgacag
     1981 tcgttcgccc aaatctttgt gctaggattc cgcgactaac ttaagccatc gcagcggtgg
     2041 ggtaatcagg acagcgcgcc gaatcacaat ccatgaccac cttcttcgca atcggaattg
     2101 tcctgaatgt cgtgctcacc ggcttggcgc tgtactggct gtggtgccag cgtatcccga
     2161 agcagggcaa ggatgaaacc ggagaatcgc gaaacgatca cggacagaat cgtcacgaac
     2221 gatccagcgc gcccgatgca tccaacaatt atctggcaga gtatgtgcgt ctgctgacag
     2281 gcagttaccg ccattggacc ggccgcgatc tggttgatcc cggcttgtca gcggtggagc
     2341 aggcgcgggc actttacgag gcgccattcg tggtggcttc gcacgatgcc gggaatgatc
     2401 cggttttcaa ttacgccaat tgcgcggcgc tggcgttgtt cgaaaccggc tggcgggatt
     2461 tcacctccat gccctcacgc ctgtcggcgg agccgatgga gcgtgagcag cgtgctcaat
     2521 tactggagcg cgtaggccgc gacggtttca tcaacgatta cagcggtatt cgtatctcca
     2581 ccactggccg acgtttccgg atccggaacg ccacggtgtg gaatcttgtg gatgcgtatg
     2641 gcgtgccgat ggggcaggcc gtgatgttcc gcgactggga atatctttag agtgtgatga
     2701 atggaccgat gcgtatcatg aacgatcagc acgcgagcga aaggaattca tcgccatgac
     2761 gacacagcgg attctgtggt acttcgccga tcccatgtgc tcgtggtgtt ggggtttcgc
     2821 tcctgtgatc ggcgccatta gggagacata ttccgaacgc atcaagatcg tcctgatgct
     2881 gggtggtctg cgcccgggta cgaccgaacc catgacgcca aaagcgcgcg aggagattct
     2941 gcaacactgg cgcgatgtgc accgcatgac cggccaacct ttcgttttcg aaggtgcgat
     3001 gcccgaggat tttgtctacg acaccgaacc gcccagccgc gccgtgatcg ctgtggccga
     3061 catcaagccc gaggctgtct ttccctattt caaatcggtg caggaggcgt tttacgcgca
     3121 cggacggaat gtcacccagc cggatacgct ggcggatctg gcggagcagc ataatatagg
     3181 gagagccatg ttcctcgatc gattcgattc ggatgacatc agaaaaaaga cacagacgca
     3241 ttttcagatc gcccgccaat cgggtgtacg cggtttcccg accgtgattc tgcagagcgg
     3301 aaccaccggc acgctcctga cgcatggctt ccgcccgttt actgagctgc aacctgacat
     3361 cgaccagtgg ctggcgcgga tggaatgacg acgcgaatta tccgcgccgt gcttggaaaa
     3421 tttccccgtg atgggtcacg cggtctgtga cggcgcatct gaacgccctg ctattccttg
     3481 acttttggca gtgagtccat aaacttcctt gcaacctgcc cccttggagg ttcaacggat
     3541 tgaccgactc gggtttgcca gcagcaccgc ccgtacaccg cgcacagcgg ggaggtggcg
     3601 tattcaccgc cacgtaactc aaccgattac cccattgcgc catgttcctg ggcgatattc
     3661 cttccgtttt tttcgtctcg caatcttttc aatagtccct gcttcatgtc atcgcgtcag
     3721 gaattttgac gcgagttggt cattgcgtcc gatgtcacgt catgccacac cgcccagtgt
     3781 catgcctcgg cggcgaaagc cgggaagaga acgggctgaa aacagagggg cgaatcatcg
     3841 cccctttttt attgaccgaa agccggaggt gcctttcata tgaaaatcat tgccgttgcc
     3901 aatcagaagg gcgggtgcgg gaaaacgact actgccatca atctcgctgc ctgtcttgga
     3961 aaaagagaac agcgggtgtt attgctcgac ctggatcccc agggccactc ctcgctcgga
     4021 ttcggagttt tcaacgagga tgcgcgcgat ctgtatgatg tttttaccgg cgagatcagg
     4081 cttgaggaaa ttattcttcc caacgttttc accggcgttg atgtcgtgcc ggcgaccaag
     4141 acgctcgccg cggcggagca cctgccggtg cgcagcgacg agcgcgataa acagctcgaa
     4201 aaacatctgg cgtcaatccg tgaccggtat gactacctcg tcatcgattg cccgccctcg
     4261 atgggactgc tttgcttgaa tggtttggcg gtatccgacc tggtgctgat cccgatcgag
     4321 atgagcctgt tcggaatgca cggcatcgac cggatgtatg aagtcatacg cgagctgcgc
     4381 gaacgccatg gacgtgaaat cccggtccgt gtactgccca cattggtcga cagccgcacg
     4441 cgcctgtgcc ggcagttcct gcacgagatc ggcgagcgct tcgccgacga tgtattgtcc
     4501 gtcatggtgc aattcacggt gcggttgaag gaagccgtgc gtcagggcgc acctatcatt
     4561 gcttacgatc cgacttcgac cgttgccgtg caatacgatc gcctggcgaa tgagatgatg
     4621 tcctcgctgc aggaaaatgt cgccactgac agcgaaggtg aggtcaagac gcgtcttaat
     4681 gccatgaaac gcgctttcga agaggcgcgc tcgcgcctgg ctggccacgc cggcaggcag
     4741 aaggtggtgc tgcgctttta cgactttgcc ggcagggatg tgaaactcgc cggcagcttc
     4801 aatgactggc gaccggatca aggggttgtc acgcgtaccg aaaagggcat cgtggagaaa
     4861 atcgtgatgc tgatgccggg aacttatcaa tatcgtttga tcgtcgatgg tatctggcag
     4921 gaggatccct ccaatcccga acaggtgtcg aattattcag gcggattcaa ttcggtgctc
     4981 cgggttgagg aggagcacga ggcggagcac gcctagcggc ggacgaagca aaagaaaatc
     5041 ccacacagtt tcttcctggc ggggaggcca cggcttccgg acagcagcgt tgacgcttta
     5101 caatgctcgc ctgtgattca tccattcccc gactttttct accgattcca caggaaccct
     5161 cggcatgact gatttatctg ccttaagcgt acgttttggc ctgtccggcc agataagctt
     5221 caaggaaatt cccggcggtt tgaccgcctt ggaagtgact aatacccatg gcagcgccac
     5281 gattgccctg caaggcgggc atgtcatgcg ctggcaaccg aaatccgagg ccgagccggt
     5341 ggtgtggtta tcacgcttcg ccaagtttgc ccccggcaaa tccatacgcg gcggcgtgcc
     5401 gctgtgctgg ccatggttcg gaccgcacgc gactgatcca aagctgccgg cgcatggatt
     5461 cgcccgtacc gtgccgtggg aggtgacagc cacgcgcgaa cttaatgatg gcgcgaccga
     5521 aatagcgctt gcgctggtgg aaacggacca gacgcgcgcc atgtggccgc atcgcacaga
     5581 gctctccatc aacatcaccg tgggacccac actcaagatt gcccttacta ccgacaatcc
     5641 cggtaacgag gatgtggtta ttggcgaggc gctgcatacc tatttccaga ttggtgatat
     5701 tgccgatatc cgtatcgagg gtctggatgg ctgtgactac ctcgacaagg taggtggcgg
     5761 cagcgtgcgc cggcatcagg acgggccggt ggttttttcc gcggagaccg accggattta
     5821 tctgaatacg gaagccaaat gcgtgattga ggatgcgcgc ctgaaacggc gcattcatgt
     5881 agcgaaatct ggcagtcgct caactgtggt gtggaatcct tggaccgaga aggccgacaa
     5941 gatgggagac ttcggcccgg agggctggcg caggatggta tgtattgaaa gtgccaacgc
     6001 ggcggagaat gtcgtggccg tcagggcggg tggccgtcat acgcttgccg tggaatattg
     6061 ggcggaaaaa ttcccagggc aatcccaacg cggcccttac tctgaatagt tgattctttt
     6121 gctatgttat tgcatcatga taaaccagtt ttaattccaa agatgtttga attcgttttt
     6181 taagagaggt ctgtaatgga aatcccgcaa cgtgacggcg atggttattt gatcgatcgc
     6241 gacaagtgga cgccggaaat tgcccgcgcc atggccgagg tcgacggtat ggcgctcgac
     6301 gaggtgaagt ggtcccacat cataaaggcg cgtgattatt acgaagaatt tggcgtggtg
     6361 ccgccgattc gcaaattcgc caagttcatc gaagaggacc agaaagacct gttcaaactc
     6421 tggatgaccg gcccgatgaa accgattacc aagtatggcg ggctgcccaa acccacaggt
     6481 tgtgtttgag aggttcagtc acggattgtc ataaaacaaa aaccccgccg agt
//
LOCUS       DBNGZE010000470         3198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_275104, whole genome shotgun sequence.
ACCESSION   DBNGZE010000470 DBNGZE010000000
VERSION     DBNGZE010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_275104"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..583
                     /locus_tag="AC16FV_12215"
     CDS             119..583
                     /locus_tag="AC16FV_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850850.1"
                     /translation="MNKGVSMHVMKSTKVFLIILITSLLAACATTARFEQMMNGYLGA
                     PIDDLQRRFGYNYIERQLPDGHRAYTWVWKEQGVFPGYETPTTIQTFSSDKGQHIIVI
                     PGTYFPSTSYETSCEFSFITDKSDKTVSWRAHGNGCAYYVGPGKILAPAAKP"
     gene            complement(794..878)
                     /locus_tag="AC16FV_12220"
     tRNA            complement(794..878)
                     /locus_tag="AC16FV_12220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Leu,seq:cag)
     gene            980..>3198
                     /gene="rnr"
                     /locus_tag="AC16FV_12225"
     CDS             980..>3198
                     /gene="rnr"
                     /locus_tag="AC16FV_12225"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR2850851.1"
                     /translation="MAEREQQKYEHPVPSREAILALLTERNEPLPFRELADVLGVHGE
                     RDEDAFSRRLRAMERDGQILKNRRGLYGLAHKMDMVSGRVSGHPDGFGFLIPDEEGED
                     LFLSPAEMRQLMHGDRVMARVTGVDQRGRREGAVVEVLERAHPTVVGRYVMQHGMGFV
                     VPSDKRITQDIAISRGDEGGARENQLVVAEIIMPPTRHRPPAGRVAEVLGDHMAPGME
                     IEVAIRAHSIPHVWPGDVLDEIRGLAPEVPAHASDGRFDLRGIPLVTIDGEDARDFDD
                     AVFAEHDGRQWRLIVAIADVSYYVKPVTALDREAYQRGNSVYFPQHVIPMLPEVLSNG
                     LCSINPKVDRLCMACEMLIDANGNISRYRFHEAVMRSRARLTYTKVAAMLVEKNAALR
                     QEYAEVAPHLEALYGLYKILHTARMKRGAVDFELPETRIVFDEQRKIREIVPLQRNDA
                     HRLIEECMLAANVCAAELLKKHKVPAPYRIHEGPTLEKLNNLREFLSEIGLSLGGGDK
                     PQAQHYSRLIKQVEKRSDSRLIQTVLLRSLSQAVYSPDNIGHFALGYPNYTHFTSPIR
                     RYPDLLVHRAIKNILVRRPAEITPELAKQQGEHCSLTERRADEATREVTRWLKAEFMM
                     DKIGEEFDGYISGVTNFGIFVELVRFYVDGLVHITALGNDYYHFDAAHHRLMGERTRQ
                     IYRLGDKVRVRVVRVDLDETKIDFELVGVSGAKTGKAPKHRRPEEKRHGKSSKKRSR"
BASE COUNT          666 a          984 c          960 g          588 t
ORIGIN      
        1 gcccagtcac ggcggttcgt tgtttgctgt cgcggttttc ccgccccttg tactactgct
       61 tgccgacccc tcataaactg aattcgggca acggttcact gtctaacttg gcgaaagcat
      121 gaacaagggt gtttccatgc acgtcatgaa atccaccaaa gtttttctga ttattctgat
      181 tacgtccctg ctggcggcat gcgctaccac tgctcgcttc gagcagatga tgaacgggta
      241 tctcggtgcg ccgatcgacg acctgcaacg tcgtttcggg tacaactata tcgagcgcca
      301 gctccccgac ggccaccgcg cctacacctg ggtctggaag gagcagggtg ttttccccgg
      361 ttacgagacg cccaccacca ttcaaacctt ttcctcggac aaggggcaac acatcatcgt
      421 catccccggc acttatttcc catcgacctc gtacgaaacc agctgcgagt tcagcttcat
      481 taccgacaag tcggataaaa ccgtgtcatg gcgcgctcat ggcaatggct gcgcctacta
      541 tgtcgggccc ggcaagatcc tggcgccagc cgcaaaacca taagccggaa atcatgccgg
      601 atcggcgtct cccccggcgc aaaatccgcc cgcggttttt gttttttgga tgggaacgag
      661 agggagaagc cggcgcgcgg gcatggtaca ctgcgcgccg ttccacttgc tcaatttaag
      721 gaaaatgctt cgcgggaagc ctgagggata cgtcttcggc ccgctaaaac gagagccgct
      781 tacagtaaaa atgtggtacc gaggagagga cttgaacctc cacgagtttc cccactagaa
      841 cctgaatcta gcgcgtctac caattccgcc acctcggcat gcagtccgtg aattatacgg
      901 gacccggcag gacggcgcac tctaccgata gataaatttc atgtcaacgc gaaaacccaa
      961 aaaaccgttt cgagacccca tggcggaacg agagcaacaa aaatacgaac atcccgttcc
     1021 cagccgcgag gccattctcg cgctgctgac ggagcgcaac gagcccctgc cgtttcgcga
     1081 actcgccgac gtgctgggtg tgcacggcga gcgcgacgag gatgccttca gtcgccgcct
     1141 gcgcgcgatg gagcgtgacg gccagatact gaaaaaccgc cgcggcctgt acggactcgc
     1201 gcacaaaatg gacatggtgt ccgggcgcgt cagcggccac ccggacggtt tcggttttct
     1261 gattcccgac gaagagggcg aagacctgtt tctttccccc gcggaaatgc gtcagctgat
     1321 gcacggcgac cgcgtgatgg cgcgcgtcac cggcgtcgat cagcgcggcc ggcgcgaggg
     1381 cgcggtagtg gaagtacttg agcgcgcgca cccgaccgtg gtcggccgtt acgtcatgca
     1441 acacggcatg ggcttcgtgg tgccgtcaga caagcgcatc actcaggata tcgcgatctc
     1501 gcgcggcgac gagggcggcg ccagggaaaa ccagctcgtg gtagcggaaa tcatcatgcc
     1561 gccgacgcgc caccgcccgc ccgcgggccg cgtggcggaa gtgctcggcg atcacatggc
     1621 gccgggcatg gagatcgagg tcgccattcg cgcgcacagc attccccatg tgtggccggg
     1681 cgatgtcctg gacgaaatca ggggactggc gcccgaggtg ccggcgcacg ccagtgacgg
     1741 gcgcttcgat ctgcgcggca ttccgctggt cacgatcgac ggcgaggacg cgcgcgattt
     1801 cgacgacgcc gtgttcgccg aacacgacgg tcgccagtgg cggctgatcg tggcgatcgc
     1861 cgatgtctcc tattacgtca agcccgtcac ggcgctggac cgagaggcct atcagcgcgg
     1921 caactcggtt tactttccgc agcacgtcat cccgatgctg ccggaggtgt tgtcgaacgg
     1981 tttgtgctcg atcaatccga aggtggaccg cctgtgcatg gcctgcgaga tgctgatcga
     2041 tgccaacggc aacatcagcc gttaccgctt tcacgaggcg gtcatgcgct cgcgtgcgcg
     2101 cctgacctac accaaggtcg cggccatgct cgtggagaaa aatgccgcat tgcgacagga
     2161 atatgccgag gtggcgccac atctcgaggc actgtatggc ctctacaaaa ttctgcacac
     2221 ggcgcgcatg aaacgcggcg cggtggattt cgaactgccg gagacgcgca tcgtgttcga
     2281 cgagcagcgc aagatccgcg agatcgtgcc gttgcaacgc aacgatgccc atcgcctgat
     2341 cgaggaatgc atgctggcgg ccaacgtctg cgccgcggaa ctcctgaaaa aacacaaggt
     2401 gcccgcgccg taccgcatcc atgaaggccc gacgctggag aaattgaata atctgcgcga
     2461 gtttctttcc gagatcggcc tttcactcgg cggcggtgac aagccgcagg cgcaacatta
     2521 ttccaggctc atcaagcagg tggagaagcg ctcggattca cgcctgatcc agaccgtgct
     2581 gctgcgttcg ctgtcgcagg cggtatacag cccagacaat atcggccact tcgcgctcgg
     2641 ttatccgaat tacacccatt tcacctcgcc gatacgccgt tatccggacc tgctggtgca
     2701 tcgcgccatc aagaacattc tggtcaggcg cccggcggag atcacgccgg agctggccaa
     2761 acagcagggc gagcactgct ccctgaccga acgccgcgcc gacgaggcca cacgcgaagt
     2821 gacccgctgg cttaaggccg agttcatgat ggacaagatc ggcgaggagt tcgacggcta
     2881 tatcagcggc gtcaccaatt tcggcatctt cgtcgagctg gtgcggttct acgtggacgg
     2941 cttggtgcac atcacggcgc tgggcaatga ttattatcac ttcgacgcgg cgcatcaccg
     3001 gctgatgggc gagcgcacgc gccagatcta ccgtctcggc gacaaggtgc gcgtgcgcgt
     3061 ggtgcgcgtg gacctggacg aaaccaagat cgatttcgaa ctggtcgggg tgagcggcgc
     3121 caaaaccggg aaggcgccca aacaccggcg tccggaagag aaacgtcacg gcaagtcttc
     3181 caaaaagcgt tcgaggaa
//
LOCUS       DBNGZE010000471         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_65457, whole genome shotgun sequence.
ACCESSION   DBNGZE010000471 DBNGZE010000000
VERSION     DBNGZE010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_65457"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..449
                     /locus_tag="AC16FV_12230"
     CDS             6..449
                     /locus_tag="AC16FV_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR2850852.1"
                     /translation="MPGLRRAVNTVRFPRPIVNQLLHLAQKSPDEEICGLISRDSRGF
                     RNCYPVPNQAGDKKRFFALDPKAQIDAMRAMRERGEELAAIYHSHPDSPPLPSLADVE
                     QHEYPAVLYLIISLGTKGVLEMRGFHIRGHAIEEIAIGLQEEPTR"
     gene            515..1090
                     /locus_tag="AC16FV_12235"
     CDS             515..1090
                     /locus_tag="AC16FV_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR2850853.1"
                     /translation="MSIATFVFLGIFGALFLYAVTMYNNFVRLKNNVAQAWSNIDVLM
                     KQRHDELPKLVEACKQYMKHERGVLEKVTQARAAVSQAREAGDVAAVGAAESQLRAGL
                     MNLFAVAENYPELRANENFQHLQNRISQLEAQITDRREFYNDSVNANNIRIETFPDNV
                     IATLFRFEPKLLLKFKENELKDVNVKALFNT"
     gene            1237..>2211
                     /locus_tag="AC16FV_12240"
     CDS             1237..>2211
                     /locus_tag="AC16FV_12240"
                     /inference="COORDINATES: protein motif:HMM:NF023899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HIR2850854.1"
                     /translation="MASSGPLAQWLSELARNINQSDPAAVGIAVALLAGACAFCSWRT
                     WRNVGRIRLIEDTPTAKIRSAPQGYVELEGMGKLMDGPPIIAKLSGLPCVWFRYKIEE
                     QVKTQHKGHTQTRWQVIDKGESTETFWLQDDTGGVVIDPEGADVTPRHKDSWRSSSML
                     GGMARLPAGVSSFFTSNTGSGTYRLTEERINSGERLYALGLLKSVSSYTSQPTVDEDV
                     REMLKDWKKDQLTLLQRFDLNKDGKIDETEWRLVRAQARRETMKNRQEQVIHSADGLS
                     VLGPTRDGSRPYILSAFTQAELIKRYKLWAALYAVGCFFLGLAAVWVFN"
BASE COUNT          481 a          723 c          615 g          392 t
ORIGIN      
        1 ccccgctgcc cggtttgcgg cgcgccgtga acacggtccg cttcccccgc cccatcgtca
       61 accagctgct gcatctggcg cagaaatcgc cggacgagga aatctgcggc ctgatctcgc
      121 gcgacagtcg cggcttccgg aattgttacc ccgtgcccaa tcaggccgga gataaaaagc
      181 gctttttcgc gctcgatccc aaggcgcaga tcgacgccat gcgcgccatg cgcgagcgcg
      241 gcgaagagct ggccgccatc tatcactccc acccagactc cccgcccctg ccctcgctgg
      301 ctgacgtcga gcagcatgaa taccccgccg tgctttacct gattatttcg ctgggcacca
      361 agggcgtgct ggaaatgcgc ggttttcaca tccgcgggca cgccatcgag gaaatcgcca
      421 tcggcctgca ggaagaaccc acccgctagc ggcgccacca acgccgtcta tactccttta
      481 caaaagccgc tgtcggcttt ctcaaggaga aaatatgtcc atcgcgacct tcgtctttct
      541 cggcatcttc ggcgcgctgt ttctctatgc cgtgaccatg tacaacaact tcgtgcggct
      601 caagaataac gtcgcacagg cctggtcgaa catcgatgtg ctgatgaagc agcgccatga
      661 cgagctgccc aagctggtcg aggcctgcaa gcagtacatg aagcacgagc gcggcgtgct
      721 ggaaaaggtc acgcaagcgc gcgcggcggt aagccaggcg cgcgaagccg gcgacgtggc
      781 tgccgtcggc gcggccgaat cccaactacg cgccggattg atgaacctgt tcgccgtcgc
      841 cgagaattac cccgagctgc gcgcgaacga gaattttcag cacctgcaaa accgcatctc
      901 gcagctcgag gcgcagatca ccgaccgccg cgagttctac aatgactcgg tcaacgccaa
      961 caacatccgc atcgagacct tcccggacaa cgtcatcgcc acgctgttcc gcttcgagcc
     1021 gaagttgctg ctcaaattca aggaaaacga gctcaaggac gtcaacgtca aggcgctctt
     1081 taatacctga caagatgacg aaaaaatcat ttttccttct ccctccggga gaaggctagg
     1141 atgaggggga cattaaaaaa tgttaaataa aatgagatcg ccctcacccc caccctctcc
     1201 cgtagggaga gggagttctt cagcaacctg ctaggcatgg cgtcctccgg tccgctggcg
     1261 cagtggctgt cggagctcgc ccgcaacatc aaccaaagcg atcccgccgc ggtcggcatt
     1321 gccgtcgcac tgctcgccgg cgcttgcgcc ttctgctcgt ggcgcacctg gcgtaacgtc
     1381 ggccgcatac gcctcattga ggacacccct accgccaaga tccgctcggc accgcagggt
     1441 tacgtggagc ttgaaggcat gggcaaactc atggacggcc cacccatcat cgccaaactc
     1501 tccggcctgc cctgtgtctg gtttcgctac aagatcgaag agcaagtcaa aacccagcac
     1561 aaaggccaca cgcagacccg ctggcaagta atcgacaaag gtgaaagcac cgaaaccttc
     1621 tggttgcagg acgacaccgg gggcgtcgtc atcgatcccg agggcgcgga cgtcacgcca
     1681 cggcacaagg acagttggcg ctccagctcc atgctgggcg gcatggcccg tctgccggcc
     1741 ggcgtctcca gctttttcac ttcgaacacc ggcagtggta cctatcgcct gaccgaggaa
     1801 cgcatcaact ccggcgagcg gctctatgcg ctcgggcttt tgaaaagcgt gagcagttac
     1861 accagccagc cgaccgtgga cgaagacgtg cgcgaaatgc tcaaggactg gaagaaagac
     1921 cagctcacgc tgttgcagcg attcgatctc aacaaggacg gaaaaatcga cgagaccgaa
     1981 tggcgcctcg tccgcgcgca ggcccggcgc gaaaccatga agaaccgcca ggaacaggtc
     2041 atccactccg ccgacggcct cagcgtcctc ggccccacgc gcgacggctc acggccgtac
     2101 atcctgtcgg ccttcacaca ggcggaattg atcaagcgtt ataaactgtg ggcagcgtta
     2161 tatgcagtgg gatgtttctt tcttggcttg gcggccgtgt gggtgttcaa c
//
LOCUS       DBNGZE010000472         2765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_622626, whole genome shotgun sequence.
ACCESSION   DBNGZE010000472 DBNGZE010000000
VERSION     DBNGZE010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_622626"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="AC16FV_12245"
     CDS             <1..825
                     /locus_tag="AC16FV_12245"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR2850855.1"
                     /translation="APVQVTWLGYPNTTGLREMDYRLTDAWADPPGASDELHTEKLIR
                     LPNGFLCYQPLSDCPEVSALPASETGHITFGCFNNSAKVNSSVLDIWSRILTDLPDAR
                     MLLKSRQLHGPGLKQRFQEEFARRGVDPGRVEMLGRVNSTFDHLALYGRVDIALDTFP
                     YNGTTTTCEALWMGVPVIALAGDRHAGRVGASLLHNVGHAELIAGSLDDYRRIANELS
                     GDRARLAAYRSSLRTHMRNAPLTNAAAFARDVETAYREMWRRWCERAASGGRTASG"
     gene            complement(842..1285)
                     /locus_tag="AC16FV_12250"
     CDS             complement(842..1285)
                     /locus_tag="AC16FV_12250"
                     /inference="COORDINATES: protein motif:HMM:NF025024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR2850856.1"
                     /translation="MNPARRSHGVVLDQESDAALFQTIFSALQRSFAAYVTKYGELRR
                     HPSVFEAFGIYRYQNETEGYDWHSDGMDPGLRYRFVSAVLYLNTVTQGGETEFLYQKR
                     KIAAQEGSVVLFPSGWTHIHRGCPPRSNPKYVLVTWTRYGDSPML"
     gene            complement(1428..2627)
                     /locus_tag="AC16FV_12255"
     CDS             complement(1428..2627)
                     /locus_tag="AC16FV_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850857.1"
                     /translation="MADHASYQEWERRAGPGMRARYADFAPRQPIFDVRPQDIPATLA
                     GYRIQTSAPDQLIVAREGTTITINGLSVSDLASALSRLDGKTTVLELAGTGAPRWPLP
                     AWRQLLQALLGTAVDLPDTFSALSAVIGRTEIVRFPVQPAHAIWRSYWENSAAVRRHL
                     PSLYRSLDDSQKFLEALARLHVLATLGENGQSYYGGYGLIPTVPGGYREIAVQTTIPE
                     TLVHTLDHWSSLLEAGPIRREGETTTPTGQTMNFVHVAGTTVVHPATGDALFSLLDEA
                     RLALKTAREALLSRRKSEAFCHLAVFHQIFVNAHPFANINNSIAMNIVNGCLREGDWA
                     WIPHLFLDYLAQRLLPERYITAFSGAMVAHGLPGPDSAAQGPPLRNSATLYSRYRADR
                     DGAASST"
BASE COUNT          535 a          819 c          894 g          517 t
ORIGIN      
        1 gcgccggtgc aggtaacgtg gctcggctat ccgaatacga ctggcctgcg cgaaatggac
       61 taccgcttga cggacgcgtg ggcggacccg ccgggcgcca gcgacgaact gcatacggag
      121 aaattgatcc gcttgccgaa cggcttcctt tgttaccagc cgttgagcga ttgtccggaa
      181 gtcagcgcgc tgcccgcaag cgaaacgggc catatcacgt tcggttgctt caacaacagc
      241 gcgaaggtca acagctccgt tctcgacatc tggagccgga ttctgaccga ccttcccgat
      301 gcgcgaatgc tgctgaaatc gcgacaactg cacgggccgg gcctgaaaca gcgctttcag
      361 gaagagtttg cccgtcgtgg cgtggatccg ggccgcgtgg agatgctggg acgcgtcaac
      421 agtactttcg accatctcgc gctttacggg cgcgtggaca ttgcgctcga tacctttcct
      481 tacaacggca ccacgacgac ctgcgaggct ttatggatgg gcgtgccggt cattgccctg
      541 gccggcgacc gccatgccgg ccgcgtcggc gcgagcctgt tgcacaacgt cgggcacgcc
      601 gagctgatcg ccggctcgct cgacgattat cggcgaatcg cgaatgagct gtcgggcgac
      661 cgcgcgcgcc tggccgcgta tcgttcgagc ctgcgaacac acatgcgtaa cgcgccgctc
      721 acgaacgccg cggctttcgc gcgcgacgtc gagacggcgt atcgcgagat gtggcgaagg
      781 tggtgcgaac gggccgcttc cggcggccga accgcttcgg gctgatatcc gcggccggat
      841 gtcacagcat cgggctgtcg ccgtaccggg tccatgtgac cagcacgtat ttcggattcg
      901 agcgaggcgg gcatccgcgg tgaatatgtg tccagccgct cgggaagagc accacgcttc
      961 cctcctgtgc cgcgattttc cgcttctggt aaaggaattc cgtttctcca ccctgtgtga
     1021 ccgtattgag atagagcacg gcgctgacga aacgataacg caggcccggg tccatcccgt
     1081 cggaatgcca gtcgtagccc tcggtttcgt tctgatagcg gtaaatcccg aacgcctcga
     1141 aaaccgacgg atgacgccgc agttccccgt atttcgtgac ataggccgcg aagctgcgct
     1201 gcaacgcgga aaaaatcgtc tggaagagcg cggcatcgct ctcctggtcc aggacaaccc
     1261 catggcttcg gcgggcgggg ttcatgacgc cggcgatgcc ggtactgctt ggagtcttgg
     1321 caggcgaccg ttcgaagtag cttatgaccc ggcggcaggt ttctgcgtcc aggacgcccg
     1381 gataaacttc aataaagtcg ctcattgttg actaaacagt tcctgattca ggtcgacgaa
     1441 gccgcgccgt cgcgatccgc gcggtagcgc gagtatagcg tggccgaatt acggagtggg
     1501 ggaccttgtg cggcggaatc cggtcccggc aagccatgag caaccattgc tccggagaat
     1561 gcagtgatgt aacgctcggg cagcagtcgc tgcgccagat aatcgaggaa gagatgcggt
     1621 atccaggccc aatcgccttc gcgcaaacag ccattcacga tgttcattgc tattgaattg
     1681 ttgatattgg cgaaaggatg ggcgttgacg aaaatctggt gaaaaacagc cagatgacaa
     1741 aacgcctcgg actttcgccg ggacagcagc gcctcgcggg cggtcttgag agcgagcctt
     1801 gcctcgtcga gcagtgaaaa aagcgcatcg ccggtcgccg ggtgtacaac ggttgttccg
     1861 gccacatgaa cgaaattcat cgtttgaccc gttggcgttg ttgtttctcc ttctctgcgg
     1921 atcggtcctg cttccaaaag cgacgaccag tggtcgagag tatggaccag cgtttccgga
     1981 atagtggtct gcaccgcgat ctcccggtaa ccgccgggga cggtgggaat cagcccgtag
     2041 ccgccataat acgactggcc gttctcacct agggtggcaa ggacgtgcaa acgggcgagc
     2101 gcctccagga atttctgcga gtcatccagc gagcggtaaa gtgacggcaa atggcggcgt
     2161 acggccgcgc tgttttccca gtaactgcgc cagatcgcat gcgccggctg cacggggaat
     2221 cgcacgattt cggtgcgccc gataaccgcc gaaagcgcgg aaaaggtgtc gggaagatcg
     2281 acggccgtgc ccagcagggc ctgtaacagc tggcgccacg ccggcagggg ccaacgcggg
     2341 gcgcctgtgc cggcgagttc aagcaccgtg gtcttgccgt cgaggcgcga cagggccgag
     2401 gcaagatcgg acaccgaaag tccgtttatc gtgatcgtcg ttccttcacg cgccactatg
     2461 agctggtcgg gggcggaggt ctgtatccgg taaccggcga gggttgccgg gatgtcctgc
     2521 ggcctgacat cgaagatcgg ctggcgcggc gcgaaatcgg cgtaacgcgc gcgcatgccg
     2581 ggaccggcac gccgttccca ttcctggtac gaggcatgat ccgccatggc gtccgttaat
     2641 gaagacatgc cggccggaaa ggcgaatgcc gccgggaccc gtaatcggtc ccggcaggaa
     2701 tgacccgccc tgtcgggcgg gcgctgacgg agcgcgtgtt catggattca cggtgatatg
     2761 ttccg
//
LOCUS       DBNGZE010000473         2234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1039288, whole genome shotgun sequence.
ACCESSION   DBNGZE010000473 DBNGZE010000000
VERSION     DBNGZE010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1039288"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC16FV_12260"
     CDS             <1..677
                     /locus_tag="AC16FV_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR2850858.1"
                     /translation="IAPQTRWAKLPDMARRGDDLLALYQMAYALFLPAFQRQLLGETA
                     RDALVDGLPEAVHSRLRLEMQSRPSLSAISVLEQRLFLGERLLRDTDAVSMAASIEVR
                     LPLVDRTLLEAVDCLPVAERYYPLRTKSLLRRIGLRGLDPALFERPKSGFELPYDRWL
                     RSGLGQAIDGTLRDADAIRATGLNPAAVQQLWQAFLDGAPGLYWSRIWALYIFIRWCH
                     RHGVFL"
     gene            1140..>2234
                     /locus_tag="AC16FV_12265"
     CDS             1140..>2234
                     /locus_tag="AC16FV_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR2850859.1"
                     /translation="MQIAIVGCGFVADLYLKTLSLHPELEITGVYDRDQDRARRFAAY
                     HHLRAYATLEELLGDPRVRIVANLTNPCSHYEVSKACLEAGKHVYSEKPLAMSLDEAK
                     HLVAVAEQRRLEISSAPCSLLSESAQTLWKCLRNDMVGKVRAVYAEMDDGLIHLMPYR
                     KWISDSGNPWPFRDEFETGCTLEHAGYNLTWLAAFFGPATDVSAFASVQIPDKAGDVP
                     AQHMAPDFSVACIRFASGVVARLTCSLIGSHDHHLRIFGDKGILWTEDTWHYRSPVYV
                     RKFITIRRRMMLNPIKRRVPLLGTHQPRPRYRGASNMDFWRGIAEMAEAVQQKRANRL
                     SGRFSLHVNELALAIHNAQGGQTYAMTTSFD"
BASE COUNT          398 a          689 c          688 g          459 t
ORIGIN      
        1 cgatcgcgcc gcaaacgcgc tgggccaagc ttcccgacat ggcacggcgc ggcgatgact
       61 tgctggcgct ctaccagatg gcgtacgcct tgttcctgcc ggcgtttcaa cggcagctgc
      121 tcggcgagac tgcccgtgac gcgctggttg acggcttgcc cgaagcggtg cattcccgtc
      181 tgcggctgga gatgcaatcg cgcccgtcat tgtcggcgat cagcgtgctg gagcagcgtc
      241 tcttcctcgg cgaacggctg ctgcgcgaca ccgacgcggt cagcatggcg gcctcgatcg
      301 aagtgcggct gccactggtg gaccggacgc tgctggaagc cgttgactgt cttcccgtgg
      361 ccgaacgcta ctacccgttg cggaccaaat ccctgctcag gcgaatcggg ctgcgcggac
      421 tcgacccggc gctgttcgag cggcccaagt ccggcttcga gctaccttac gaccgctggc
      481 tgcgcagcgg tctcggccag gcgattgacg gcaccttgcg cgatgccgat gcgatccgcg
      541 ccacggggct caatcccgcc gccgtgcagc aattgtggca agcctttctg gacggtgcgc
      601 ccggcctgta ctggtcgcgt atctgggccc tgtatatctt tatccgctgg tgccatcgtc
      661 atggcgtgtt cctgtagaat tggaaccaac tcccacgcaa tctttaaaat atggtagcct
      721 ctgctgaccg gtggcagggg gtgaggcaag agagcagagc aagagttccg gtgatcagtc
      781 caaggccatg gcggcgtgaa gccatccggg aactcaagat gatgaaggag gcgcaccgga
      841 aaccgccgtc atccgacgca ggcgggctgt gcgagaaaga gctgcaagat cggacggcac
      901 gtgcatttat taagtcaacg ttgaacgccc aatccaatcg tttccccagg gatgtggatg
      961 tttctcgacc cttcacgtga ggccgcggtg cgcgttcgca ccgaccgctg cttgaggtgg
     1021 tggatgaatt attgttctta tgtaacgggg agggcgcttt ttggagccac ttcagtctgg
     1081 cgtccctgcg gaagcgcggt atcctaaaca tccggtagtc cggtatttgg agggtgtgaa
     1141 tgcaaatcgc gatcgtaggt tgcggctttg tagccgatct ttatctgaag accctgtctt
     1201 tgcatccgga gcttgagatc acgggtgtct acgaccgcga tcaggatcgg gcccgccgtt
     1261 ttgccgccta tcaccatctc cgggcctatg cgacgctcga ggagctgctt ggagatcccc
     1321 gcgttcggat cgtcgcgaat ctaaccaatc cgtgctccca ttacgaagtt tccaaggcct
     1381 gcctcgaggc cggcaagcat gtctactccg agaagcctct ggcgatgtcg ctggacgagg
     1441 ccaagcacct ggttgccgtc gccgagcagc gcaggctgga gatatcgtcc gccccatgtt
     1501 cgcttctgag cgaatccgca cagaccctgt ggaagtgcct gcgcaacgac atggtcggca
     1561 aagtccgcgc ggtgtacgcg gaaatggacg acggcctgat tcatctcatg ccctaccgta
     1621 aatggattag cgattccggc aacccctggc cgttccggga tgaattcgag actggctgca
     1681 cgctcgagca tgccggctac aatctgactt ggctggcggc ctttttcgga ccggctaccg
     1741 acgtgtcggc gtttgcctcc gtgcaaatcc ccgacaaggc tggcgacgtg ccggcgcaac
     1801 acatggcgcc cgacttttcc gttgcctgca tccgtttcgc cagcggcgtc gtcgcgcgtc
     1861 tgacgtgcag tctcatcggg tcccacgatc accatttgcg gatcttcggc gacaagggca
     1921 tcctctggac cgaagacacc tggcactatc gctcgccggt ttacgtgcgc aagttcatca
     1981 ctatccgccg acggatgatg ctcaatccga tcaagcgccg ggtgccgttg ctgggcacgc
     2041 atcagccgcg cccgagatac cggggcgcgt cgaacatgga tttctggcgc gggatcgccg
     2101 agatggccga agcggtgcag cagaaacgcg ccaaccggct ttccggccgc ttttcgcttc
     2161 acgtcaacga gctggccctg gctattcaca acgcgcaggg cgggcaaacg tatgccatga
     2221 ccacgtcttt cgac
//
LOCUS       DBNGZE010000474         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_623067, whole genome shotgun sequence.
ACCESSION   DBNGZE010000474 DBNGZE010000000
VERSION     DBNGZE010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_623067"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16FV_12270"
     CDS             complement(<1..464)
                     /locus_tag="AC16FV_12270"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR2850860.1"
                     /translation="MAKDPVNNLNVLSQDLLPTPQQVKNALPLTEKAQATVLQGRETV
                     RRILDREDPRLFVVIGPCSIQDIKAAHEYAKRLKRLAEEVGDTLYLIMRVYFEKPRTT
                     VGWKGLINDPFMDDSFHIEKGLHLARELLLHLAEMGLPTATEALDPITPLYLS"
     gene            complement(639..1643)
                     /locus_tag="AC16FV_12275"
     CDS             complement(639..1643)
                     /locus_tag="AC16FV_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR2850861.1"
                     /translation="MSRDTLSDLLRSVRVRGAVFYYVSCRDQWSAEAPPAKEIAEAVL
                     PGCEHVMEYHMIAKGNGWAAISGQAPVRLATGDIVMFPQGDRHVLSSAPNVEPLRMTA
                     DWVFARRNDPKPMPISYHHGVVEAGAPMPVENADMVAVCGFLGCDLRPFNPLVAALPR
                     VLHLPASHAGGWVSRVIEQAVVESNSPRPGGDAVLERLAEMMFVDAARRYLDSLPEDA
                     TGWLAGLRDRFVGKALALMHERPDHPWSLDDLSHEVGLSRSALHERFVEFVGHPPMQY
                     LASWRIQLGARLLRESNRTVATIALDVGYDSEAAFSRAFKRMVGVPPAAWRKAQARAE
                     "
     gene            1888..2121
                     /locus_tag="AC16FV_12280"
     CDS             1888..2121
                     /locus_tag="AC16FV_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850862.1"
                     /translation="MQATQANVVVDVKQVLNRQSGAELVSDLGGMQGVSRASVSRRAP
                     RLVLVDYDPKVTDSRRILGTVVRHGFDARLIGM"
     gene            2191..3060
                     /locus_tag="AC16FV_12285"
     CDS             2191..3060
                     /locus_tag="AC16FV_12285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850863.1"
                     /translation="MRAELKVCTPATSAKASTAANANVDFNAIKQRQQATWASGDYTI
                     IGTTLQIVGESLAEAADVRADERVVDVAAGNGNATLAAARRFARVTSTDYVPHLLEKG
                     AARAKAEGLDIRFQVADAEALPFEDGSFDVALSTFGAMFTPEHGKAAREMTRVVRSGG
                     RIGMANWTPEGFIGQLFRVSGAHVPPPAGVQSPTLWGNEPRIVELFGSEATDIRCERR
                     HFTFRYRSAAHWVEVFRSFYGPTHKAYAALDPARQARLTEDITALLERLNVGGPDSLV
                     VPSEYLEVVITKK"
BASE COUNT          629 a         1020 c          998 g          548 t
ORIGIN      
        1 gagaggtata gcggcgtgat cgggtcgagc gcctcggtcg cggtcggcag gcccatctcc
       61 gccagatgca gcaacagctc gcgcgccagg tgcaggccct tctcgatatg gaaggaatcg
      121 tccatgaacg gatcgttgat cagccccttc cagccgacgg tggtgcgcgg cttctcgaaa
      181 tacacgcgca tgatgagata gagcgtgtcg cccacctcct ccgccagccg cttcaggcgc
      241 ttggcatatt catgggccgc cttgatgtct tggatcgaac acggcccgat gaccacgaac
      301 aggcgcgggt cctcacggtc caggatgcgc cgcaccgtct cgcgcccctg cagcaccgtc
      361 gcctgcgcct tctccgtcag cggcagcgcg ttcttcacct gctgcggcgt cggcagcaga
      421 tcctgcgaga ggacgtttag attgttaacc gggtcttttg ccatgggtaa tgcctgagaa
      481 agccggcaag tttaacgtgt tgcgcgtcct tcccaaaatc gcccgaccgg atcagcggac
      541 ggcgacggga gcttgcatga aggccctgga aaaggccttt gacgagacaa aacaaggggg
      601 acggacagtg tttgagattg aattcccgct atcggccatt actccgcacg cgcctgtgcc
      661 ttccgccacg cggccggcgg cacgccgacc atccgcttga acgcacgcga gaacgctgcc
      721 tcggaatcgt agccgacatc tagtgcaatc gtcgcgacgg tgcgattcga ttcgcgcaac
      781 agccgcgccc cgagctggat gcgccaactg gcaagatatt gcatcggcgg atgaccgacg
      841 aactccacga agcgttcatg gagcgcggaa cgcgacaagc cgacctcgtg cgaaagatcg
      901 tcgagactcc acgggtgatc aggacgttcg tgcatgagcg cgagcgcctt gccgacgaaa
      961 cggtcgcgca gccccgcgag ccagccggtc gcatcctcgg gcaggctgtc gagatagcgg
     1021 cgggcggcat caacgaacat catctccgca agtcgttcga gcacggcgtc accaccgggt
     1081 cgtgggctgt tcgattcgac taccgcctgt tcaatcacgc gagacaccca accgcccgca
     1141 tgcgacgccg gcagatgcag gacccgcggc agcgccgcga ccagcgggtt aaacggccgc
     1201 agatcacagc cgaggaatcc gcacacggcg accatatcgg cgttttcgac gggcatcggc
     1261 gcgcccgcct ccaccacgcc atggtgatag gagatcggca tcggcttcgg gtcgttccgc
     1321 cgggcaaaca cccaatcggc ggtcatgcgc agcggctcca cgttcggcgc gctcgacagt
     1381 acgtgacgat cgccttgcgg aaacatcacg atgtcaccgg tggcaagtct cacgggcgcc
     1441 tggcccgaga tggctgccca gccgttgccc ttcgcgatca tgtggtactc catcacatgc
     1501 tcgcagccgg gcaacacggc ttcggcgatt tccttcgccg gcggcgcctc ggcagaccac
     1561 tggtcgcgac agctcacgta atagaacacc gcgccgcgca cgcgcacgct gcgtaacagg
     1621 tcggaaagcg tgtctcggct catcaagatt gaccggtggg tggagaaata atcaagaaac
     1681 gtggacacac gatcaagtta ttcatcaatc gcggacgcct gatcaagttt gcccgacgcc
     1741 cagtgaagcg tcaacgaaat ccttccgcaa caattgcctc actgagaatc atcgctggta
     1801 cgtgcaagga cgattcaagg cgggaaggtt gatccgcaaa tggacgaccg gcccaaatcc
     1861 aatcatcgtc aaaaggagac aagtgtcatg caagcaactc aggccaacgt agtagtggac
     1921 gtgaagcagg tgctcaaccg ccaatccggc gccgagctcg taagcgatct cggcgggatg
     1981 caaggcgtga gccgggcgtc ggtgagtcgg cgggcgcctc gactggtgct cgtggactac
     2041 gaccccaagg tgaccgactc gcggaggatc ctcggcaccg tcgtgcgcca cggattcgat
     2101 gcgcggctta tcggcatgta atcaggggcc cggaccgtcg gccatcgtga tgggtgggct
     2161 taattttcaa ccattatttg gagaatccac atgagagcag aactgaaagt ttgtactccg
     2221 gccacatcgg ccaaggcgtc gaccgcggcc aatgccaatg tcgatttcaa cgccatcaag
     2281 caacgtcagc aggccacgtg ggccagtggc gactacacga tcatcggcac cacgctccag
     2341 attgtcggcg agtcgctggc ggaggcggct gacgtgcgcg ccgatgagcg cgtcgtggac
     2401 gtggccgccg gcaacggcaa cgccacactg gcggccgccc gccgcttcgc gcgcgtgacc
     2461 tcgaccgact atgtgccgca cctgctcgag aaaggggcgg cgcgggcaaa ggccgaaggg
     2521 ctggacatcc ggtttcaggt ggcggatgcc gaggcattgc catttgaaga tggatcgttc
     2581 gacgtggcgc tgtccacctt cggcgcgatg ttcacgcccg agcacggcaa ggcggcgcgc
     2641 gagatgacgc gcgtggtgcg cagcggcggc cgcatcggca tggcgaactg gacgcccgaa
     2701 ggtttcatcg gccagctctt tcgcgtgagc ggcgcccacg tcccgccgcc ggcgggcgtg
     2761 caatccccga cgctgtgggg caacgagccg cggatcgtcg aattgttcgg ttcggaagcg
     2821 accgacattc gctgtgagcg acgtcacttc acctttcgct atcgctccgc cgcccactgg
     2881 gtcgaggtgt tccgcagctt ctacggaccg acgcacaagg cctacgcggc gcttgatccc
     2941 gctcgccagg cgcgcttgac cgaagacatt accgctctcc tcgagcgcct gaatgtaggc
     3001 gggccggact cgctcgtcgt gcccagcgag tacctcgaag tggtcatcac gaagaagtaa
     3061 cgccgggggt caggtcacat agtcacatgt attagcggga accgtgatct gaccccaatt
     3121 gtttgcaaat tgatctgtcc cggattaccc gttcggaccg ctgcgcgcct ggcgccgcac
     3181 gtcgcccggt ccgac
//
LOCUS       DBNGZE010000475         7777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_689578, whole genome shotgun sequence.
ACCESSION   DBNGZE010000475 DBNGZE010000000
VERSION     DBNGZE010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_689578"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC16FV_12290"
     CDS             complement(<1..247)
                     /locus_tag="AC16FV_12290"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR2850864.1"
                     /translation="MNERRDSQRVPVSLDAVLNYQAQVMICTIRDISLNGAFIEGTPE
                     GLPYFNAPVELGLTLTSGGETKQHRIPAKIRRITDNGV"
     gene            complement(461..1297)
                     /locus_tag="AC16FV_12295"
     CDS             complement(461..1297)
                     /locus_tag="AC16FV_12295"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HIR2850865.1"
                     /translation="MNYRHIFHAGNFADVFKHIVLSLLLKSLHGKDKPFCYLDTHAGA
                     GRYDLSSAAAQKTGEYRDGIKRLLNGQTMPVLADYLAAVRALNDAGSLRYYPGSPRIA
                     RFFLRPQDRAILLELQPEECTQLKAEFAGDRQVIVQGQDGYAGLKAFLPPKERRGLVL
                     IDPPYESDKEFERVIEGLRLAYARWDSGIYALWYPIKSRPPVERFHRMLVATGIRKIL
                     LAEFSPWPEDTVFRLNGCGMVVINPPWQLDETLHSVLPALLEVLRQHPAGRCAVTWLV
                     PE"
     gene            complement(1332..2345)
                     /gene="hemB"
                     /locus_tag="AC16FV_12300"
     CDS             complement(1332..2345)
                     /gene="hemB"
                     /locus_tag="AC16FV_12300"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005493052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR2850866.1"
                     /translation="MATGTGKFPRVRMRRMRRDDFSRRLMRETRLTTDDLIQPVFVME
                     GKNRAEAVPSMPGISRVTTDLLLKQAEKLLKLDVPAIALFPVVPTSKKDNKAKEAFNP
                     NGLVPQTVHALKKNFPELGVITDIALDPYTSHGQDGLIDGTGYVLNDETVAVLVKQAL
                     SHAAAGADVVAPSDMMDGRIGAIRDALEKAGHIHTRILAYSAKYASNFYGPFRDAVGS
                     AANLDKSNKDNYQMDPANSDEALREVALDIDEGADMVMVKPGLPYLDIVRRVKDKFGV
                     PTFVYQVSGEYAMLMAAAKNGWLDERTCVMESLLCIKRAGADAILTYFADRAAGWLKE
                     GSH"
     gene            complement(2762..3403)
                     /locus_tag="AC16FV_12305"
                     /pseudo
     CDS             complement(2762..3403)
                     /locus_tag="AC16FV_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
     gene            3623..4042
                     /locus_tag="AC16FV_12310"
     CDS             3623..4042
                     /locus_tag="AC16FV_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850867.1"
                     /translation="MTHNKIPDFTETELWAVRSTLKERYGKEVEPQLADTEVMLGGAD
                     GMVWCPALFWTAKGANFAIVKIGAKRFRAYFYYHPEHQLGTGIDSYDEIGDCVISVLQ
                     VESDHIRKQKISTDQNPAQTDKDSPEKPDLTPNFWGD"
     gene            4048..5319
                     /locus_tag="AC16FV_12315"
     CDS             4048..5319
                     /locus_tag="AC16FV_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR2850868.1"
                     /translation="MAKSKPKNAFYAQSGGVTAVINASACGVLETARKYPKKIGKVYA
                     GRNGIIGALTEDLINTSKESAAAIKALRHTPSGAFGSCRYKLKSLEQNKREYDRLIEV
                     FKAHDIGYFFYNGGGDSADTCLKISQLSETMGYPIQAIHVPKTVDNDLPITDNCPGFG
                     SVAKYIAVSTREASFDVASMAKTSTKVFILEVMGRHAGWIAAAGGLASTKDTEIPIVI
                     LFPEVSFDKAKFLATVDANVKKFGYCTVVVSEGVKSPDGKFLADQGLKDAFGHAQLGG
                     VAPVVASIIKEGLGLKYHWGVADYLQRAARHIASKSDVDQACAMGKAAVELAVKGKNS
                     VMPTVERVSSKPYKWKVGVAPLNKVANVEKMMPKDFITDDGFGITAKCRTYLEPLIQG
                     EDYPPYKNGMPQYVRLKNVPVKKKLPGEFKP"
     gene            5335..5547
                     /locus_tag="AC16FV_12320"
     CDS             5335..5547
                     /locus_tag="AC16FV_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850869.1"
                     /translation="MTLDKATELLKVQAGFGGFYNANSAKLILAEVSREHGQAAVDRL
                     IRELNLDKVFNFEPGMRFEGGLATKK"
     gene            5669..7690
                     /locus_tag="AC16FV_12325"
     CDS             5669..7690
                     /locus_tag="AC16FV_12325"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR2850870.1"
                     /translation="MSDSLIFALACAIAAIVYGVMSIKWVLNKPTGSDRMREIAAAIQ
                     EGAKAYLNRQYSTIAIVGVILFLVIYFALDPRTAIGFAIGSLLSAATGYIGMNISVRA
                     NVRTTEAAKDGLNPALQVAFRAGAITGLFVVGLGLLGVAGYYAILAHLYPDSSSSNLI
                     APLVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTIIATMLLGSLLLPAFGNAVLYPLVLGGVSILASIVGTF
                     FVKTSGKGKIMNALYRAVIVSGVLAAIAFYFVTNKMMAGNGAYATMNIYGAALIGLAL
                     TTAMVVITEYYTATEYSPVRKIAQASTTGHGTNIIAGLGLSMKATALPVLAVCASIWG
                     AYHLAGLYGIAIAATSMLSMTGVIVALDAYGPVTDNGGGIAEMAGLPKKVRDVTDPLD
                     AVGNTTKAVTKGYAIGSAGLAALVLFADYTDALKHAGKAVEFSLSNPNVIIGLFIGGL
                     IPYLFGALAMEAVGRAAGSVVVEVRRQFKTIKGIMEGKAKPDYSKAVDMLTKAAIKEM
                     IIPSLLPVLVPIVVGFTLGKEALGAVLLGTIVTGIFVAISMTTGGGAWDNAKKYIEDG
                     KHGGKGSDAHKAAVTGDTVGDPYKDTAGPAINPMIKIINIVALLIVPLL"
BASE COUNT         1544 a         2410 c         2264 g         1559 t
ORIGIN      
        1 cgacgccatt gtcggtaatg cggcggatct tggcgggaat gcgatgttgc ttggtctcac
       61 cgccggaagt cagcgtcaga cccagttcca cgggggcgtt gaaatacggc agtccttccg
      121 gggtaccctc gataaaagcg ccattcaggc tgatatcccg gatggtgcag atcatgacct
      181 gcgcttgata attcagcacg gcatcaagcg aaaccggcac gcgctgactg tcgcgtcgtt
      241 cgttcatggc gttgctgttc cccccaatag ccggcaagcc tttcaccact ggaacctcca
      301 cgccgaaaac ccggcaaaag ctgttttatt attgaaaaac ctgtacttaa tggtgaataa
      361 aatacccggt tagacgcgcg ctgtcgacgc ctgtttgtaa caggtggttg ccgttgcact
      421 tttaccgctg gtcagcattg ccatcggcgc tattgcgagc ttattccggc accagccagg
      481 ttaccgcgca acgtccagcc ggatgttgcc gcagcacctc cagcagcgcc ggcaacactg
      541 aatggagcgt ttcatccagc tgccagggag gattgatcac gaccatgccg caaccgttaa
      601 ggcggaagac cgtatcctcg ggccaggggc tgaattccgc cagcaggatt ttacgaattc
      661 cggtggcgac cagcatgcgg tgaaaccgct ccaccggcgg gcggctcttg atggggtacc
      721 acaaggcgta tatcccggaa tcccagcgcg cataggccag gcgcaaacct tctataaccc
      781 gctcaaactc cttgtccgat tcgtagggcg gatcgatcag caccagaccg cgtctttcct
      841 ttggcggcaa gaatgccttc aggccggcat agccatcctg accctgaaca atcacctgcc
      901 gatccccggc aaattcggct ttgagctgcg tgcattcctc cggctgaagc tcgagcagta
      961 tcgcgcggtc ctgcggtctc agaaaaaaac gcgcgatgcg tggcgatccg gggtaatacc
     1021 gcaagctgcc cgcgtcattc aacgcgcgca ctgcggccag atagtccgcc agcacgggca
     1081 tggtttgacc attcaacaga cgtttgatgc cgtcacggta ctcgccggtt ttttgcgccg
     1141 ccgcgctgct gagatcgtag cggcccgcac cggcgtgggt atcgagatag caaaacggct
     1201 tgtctttgcc gtgcagagac ttgagcaaca acgaaagcac gatgtgtttg aagacatcgg
     1261 caaaattgcc ggcatggaag atatggcggt aattcatgag gacagcgtgc cgcgcgtcgc
     1321 gggcggccgg atcagtgcga gccttctttc agccacccgg cggcacgatc ggcgaaatag
     1381 gtgagaatcg catccgcgcc ggcgcgctta atgcaaagca gcgattccat gacgcaggtg
     1441 cgttcgtcga gccagccgtt tttcgccgcc gccatgagca tggcgtattc gccgctgacc
     1501 tggtaaacga aagtcggcac gccgaatttg tccttcaccc ggcgcacgat gtcgagatac
     1561 ggcagtccgg gcttgaccat caccatgtcc gcgccctcat cgatgtcgag tgccacttcg
     1621 cgcagtgcct catccgaatt ggccgggtcc atctggtagt tgtccttgtt gctcttgtcg
     1681 agattggcgg ccgaacccac ggcgtcgcgg aaagggccgt aaaaattcga ggcatatttc
     1741 gcggaataag ccaggatgcg ggtatgaata tgccccgcct tctctaatgc gtcgcgaatg
     1801 gcgccaatgc gtccgtccat catgtccgag ggcgccacga catcggcccc ggcggcggca
     1861 tgcgacagcg cctgcttcac cagcaccgcc acggtctcgt cgttcagcac gtaacccgtg
     1921 ccgtcgatca gaccgtcttg gccatgcgag gtataaggat cgagcgcgat gtcggtaatc
     1981 acgcccagtt ccgggaaatt tttcttgagc gcatgcaccg tctgcggtac aagcccattg
     2041 ggattgaagg cttccttggc cttgttgtct tttttcgacg tcggcaccac cgggaacagg
     2101 gcaatagctg gcacatccag cttcaggagt ttttccgcct gcttgagcag caggtcggtc
     2161 gtcacacggc tgatgcccgg catgcttggc accgcttccg cacggttctt gccttccatg
     2221 acgaacaccg gctggatgag atcgtccgtt gtgagccggg tctcgcgcat taaccgacgc
     2281 gaaaaatcat cgcgccgcat gcgccgcatg cgtactcgcg ggaattttcc ggtgccggtt
     2341 gccatgaggt ccgagaaagg gtggtgattt ttccttatgc tagcttagcc gtctgccggg
     2401 tgaaatgccg acacaagctt ttcaaaacag cagggcccgg tctcccgggc cctgctgatg
     2461 aatccaatac ttacttgacc tttttggcct tggcgacggg tttggccgct tttttgggtt
     2521 tggccttggt cttggacggc ggggcctttt tcggagcgac ctttttcgca accggcttgg
     2581 ccttgggcgg cggcaccggc ttggccttgg gtttgacgac aggcgccggt ttggccgcct
     2641 tggcggcctt cgccgctttt gccgcggcct tggccttggc ctcttccgcc ttggccttgg
     2701 cggcttggcg cgccgccgcc gactcgatct tgttcatcac gtccgccaag ggccggattt
     2761 gcgactcgat gatatcggta gcgcttttga agatggcgct caccgtgccc acgccgcgca
     2821 cggtgcggag cttgccttgc gccttgtaat agttggcgag cggcgcggtc tgttgctgat
     2881 acacgtcgag gcggttacgc accgtctgct cgttatcgtc ggaacggtgc gtcagaccgc
     2941 cgccgcactt gtcgcatttg ttggaggttt tcggggccga gaaatgaatg ttgtaaatcg
     3001 cgccacagga accgcaggtg cgccgtccgg tcaggcgctt catcaataca tcggtgtcga
     3061 catccaccag cagcgcgagc tgcaagggtt gactgagacg cgccagcatg gcgtcgagcg
     3121 cctgcgcctg tggaatattg cgcggaaaac cgtcgaggat aaaaccgctc ttggcgtcag
     3181 gcttggcaag ccgcgcctgg atcatgccga gcacaatgtc atcggtaacc agctggccgg
     3241 catccatcgc ggccttggcc tttttcccca gatcggttcc ggccgccact tccgcgcgca
     3301 acaggtcgcc tgtggacatc tgcggaattt tgtacttctc gaccaataac ttagcttgcg
     3361 ttcccttgcc ggaccccggc gctcccaaaa gcacgattct catggcattc tcgcttttaa
     3421 gttcttggtg gtgcgtattc tgaaggattt ttccctggtt ctatgtcagg ggcgcgtaaa
     3481 ctacaatttg cccaccccgg cgtcaatggt ccgcgcccta taaaagggcc ctattgccgg
     3541 attcatcttt ccgcgtgttc caaggaggaa aacttggtta agctcgtttt cgcaggcggg
     3601 gtatcgccct gaattccatc cgatgactca taacaaaatt ccggatttta ccgagaccga
     3661 gctctgggcc gtgcgcagca ccctgaagga acgttacggc aaggaagtcg aaccgcaact
     3721 ggccgacacc gaggtcatgc tcggcggcgc cgacggcatg gtctggtgcc cggcgctgtt
     3781 ctggactgcc aagggtgcga acttcgccat tgtcaaaatc ggcgccaagc gctttcgggc
     3841 ctacttctat tatcatccgg agcatcagct cggcaccggc atcgacagtt acgatgaaat
     3901 cggcgactgc gtgatcagcg tcctgcaggt cgagtccgat cacattcgca agcagaaaat
     3961 cagcaccgat cagaacccgg cgcagactga caaggactcg ccggagaaac cggatttaac
     4021 accaaacttc tggggagatt gaaaccaatg gcaaaaagca aacccaaaaa cgcgttctat
     4081 gcccagtcgg gcggcgtcac cgcggtcatc aacgcctcgg cctgcggcgt gctcgaaacc
     4141 gcgcgcaagt acccgaagaa aatcggcaag gtctatgccg gccgcaacgg catcatcggc
     4201 gcgctcaccg aggacctgat caacaccagc aaggaatcgg cggccgccat caaggcgctg
     4261 cgccacacgc cctcgggcgc cttcggctcc tgccgctaca agttgaaaag cctggaacag
     4321 aacaagcgtg agtacgaccg cctgatcgag gtattcaagg cgcacgacat cggttacttc
     4381 ttttacaacg gcggtggcga ttcggccgac acctgcctca agatctcgca actttccgaa
     4441 accatgggtt atccgatcca ggccattcac gtgcccaaaa ccgtggacaa cgacctgccc
     4501 atcaccgaca actgcccggg cttcggctcg gtcgccaaat acatcgctgt atcgacgcgc
     4561 gaggcctcgt tcgacgtggc ctcgatggcc aagacctcaa ccaaggtgtt catcctcgaa
     4621 gtcatgggcc ggcacgccgg ctggatcgcc gccgcgggcg gactggcctc gaccaaggac
     4681 accgaaatcc cgatcgtgat cctgttcccg gaagtgagtt tcgacaaggc aaaattcctc
     4741 gccaccgttg atgccaacgt caaaaaattc ggctactgca cggtagtagt atccgaaggc
     4801 gtgaaaagcc cggacggcaa attccttgcc gatcagggcc tcaaggacgc ctttggccac
     4861 gcccagctgg gcggagtcgc gccggtggtc gcgtccatta tcaaggaagg gctcggcctc
     4921 aagtatcact ggggtgtcgc cgattatctt cagcgcgccg cgcgtcacat cgcttccaag
     4981 agcgacgtgg accaggcttg cgccatgggc aaggcggcgg tggagctggc cgtcaagggc
     5041 aagaactccg tgatgccaac cgtggagcgc gtgtcgagca aaccttataa atggaaggtc
     5101 ggcgttgcgc ctcttaataa agtggcgaac gtggaaaaaa tgatgcccaa ggatttcatc
     5161 accgacgacg gcttcggcat caccgccaag tgtcgcacct atctggagcc gctgatccag
     5221 ggcgaagact atccgccata taagaacggc atgccgcagt acgtgcgtct caagaacgta
     5281 ccggtgaaga agaaactccc gggcgaattc aaaccctgaa acaccggcac ccgcatgaca
     5341 ctcgacaagg caacggaatt gcttaaagtg caggcgggtt tcggcgggtt ctacaacgcc
     5401 aattcagcca agctgattct cgcggaagtc agtcgcgagc acggccaggc ggcggtggac
     5461 cggctgatcc gtgaattgaa cctggacaag gtattcaatt tcgagccggg catgcgcttc
     5521 gagggcggac tggctacgaa aaaatgaaca tccggcggca attgattttt atcaacgatg
     5581 cggccttttt ttatcggtta tttttatcac cggccgaaaa ccaatgcgcc cacgggcgcg
     5641 gcaataccaa cttgcgggag gaaaacacat gtctgacagc ctgatcttcg ccctggcgtg
     5701 cgccattgcg gccatcgttt atggtgtgat gtccatcaaa tgggttctga acaaacccac
     5761 cggcagcgac cgcatgcgcg agatcgcggc cgcgatccag gaaggcgcca aggcctacct
     5821 gaaccgccag tacagcacca tcgccattgt cggcgtaatc ctgttcctcg tgatttactt
     5881 cgcactcgat ccgcgcaccg ccatcggctt cgccatcggc tcgctgctct ccgccgccac
     5941 cggttacatc ggcatgaaca tctcggtgcg tgccaacgtg cgcactactg aagcggccaa
     6001 ggacgggctc aacccggcat tgcaggtcgc gttccgcgcg ggcgccatta ccggtctgtt
     6061 cgtggtcggc ctcgggctgc tgggcgtcgc cggctactac gccattctgg cgcatctcta
     6121 tcctgattcc tcgtcatcga atctgatcgc gcctctggtc gggctcgcct tcggcggctc
     6181 gctcatttcg atcttcgcgc gcctcggcgg cggcatcttc accaagggcg cggacgtggg
     6241 cgccgacctc gtcggcaagg tcgaggccgg cataccggag gacgacccgc gcaacccggc
     6301 ggtgattgcc gacaacgtgg gcgacaacgt cggcgactgt gccggcatgg ctgccgacct
     6361 gttcgagacc tatgcggtga ccatcatcgc caccatgctg ctcggcagcc tgctgctgcc
     6421 ggcattcggc aatgcggtgc tgtacccgct ggtactgggc ggcgtgtcca tcctcgcctc
     6481 catcgtcggc accttcttcg tgaaaacctc cggcaagggc aaaatcatga acgccctcta
     6541 tcgcgccgtg atcgtctccg gcgtgctcgc ggccatcgcg ttctacttcg tcaccaacaa
     6601 gatgatggcg ggcaacggcg cctacgccac aatgaacatc tacggcgcgg cgctcatcgg
     6661 gctggcgctc accaccgcca tggttgtcat caccgagtac tacaccgcga ccgagtacag
     6721 tccggtacgc aagattgcgc aggcctcgac cacaggccac ggcaccaaca tcatcgccgg
     6781 actgggttta tccatgaagg ccacggcgct gccggtgctc gcggtgtgcg ctagcatctg
     6841 gggcgcgtat cacctcgccg gcctatacgg catcgccatc gccgccacct cgatgctgtc
     6901 catgaccggc gtcatcgtgg cgctcgacgc ctacggtccg gtcaccgaca acggcggcgg
     6961 catcgccgag atggcgggct tgccgaaaaa ggtgcgtgat gtgaccgatc cgctcgacgc
     7021 cgtaggcaat accaccaagg ccgtgaccaa gggctatgcc atcggctcgg ccggcctcgc
     7081 ggcgctggtg ctgttcgcgg actacaccga cgcgctcaag cacgccggca aggcggtgga
     7141 attcagcctg tccaatccca acgtgattat cggcctgttc atcggtgggc tcatccccta
     7201 cctcttcggc gcgcttgcca tggaggcggt cggtcgcgcc gccggcagcg tcgtggtcga
     7261 ggtgcggcgc cagttcaaga ccatcaaggg catcatggaa ggcaaggcca agccggatta
     7321 ttcgaaggcg gtggacatgc tcaccaaggc cgcgatcaag gagatgatca ttccgtcgct
     7381 gcttccggtg ctggtgccga tcgtcgtcgg cttcacgctc ggcaaggagg cgctgggcgc
     7441 ggtgctgctc ggcaccatcg tcaccggcat tttcgtcgcc atctccatga ccaccggcgg
     7501 cggcgcctgg gacaacgcca agaaatacat cgaggacggc aagcacggcg gcaaaggctc
     7561 ggacgcgcac aaggccgccg tcaccggcga caccgtgggc gatccctaca aggacaccgc
     7621 cggcccggcg atcaacccca tgatcaagat catcaacatc gtggcattgc tgatcgtacc
     7681 gttactttaa aaacaaaaga cggtcgccgg cgataccgtg gacgcgatca gccccatgat
     7741 aaatatcctc atcatcgtgg cgcagctgat cgtgcca
//
LOCUS       DBNGZE010000476         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1456355, whole genome shotgun sequence.
ACCESSION   DBNGZE010000476 DBNGZE010000000
VERSION     DBNGZE010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1456355"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="AC16FV_12330"
     CDS             <1..1204
                     /locus_tag="AC16FV_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR2850871.1"
                     /translation="IPLLWRQQDIVEALRRVDAKAIVTLPRIGTTAYAELAMQAAVEL
                     FPIRHVCSFGANLPDGIVSLDEAFASGSVDVAAPYIRPGPAAAHVAAITFGLDAKGLV
                     ELVAAGLETFLEAGAEADTSLLSTIPIGSFAGLALTLVYWLLSGGALHLHHGFDPDAF
                     DAQSGSLTGGTVVLPASALASVAEAGLLREAHRTIVALWRAPERLTSAKPWHGKPTLV
                     DVASFGEIGLVAARRGENRLPTAIRHGVVNSSHRAPGAPVVIETARSDVGTLVLRGRM
                     VSVHAFAPGAKSAYPPRLSSDSAGYVDTGFACRLDRNAQTLTITAPPSGTIAVGGYCF
                     RANLIDALIADAELDATLVALPDSDLGQRFAGAAADRATLRAKLQAQGINPLISGAFV
                     LRGAPEAA"
     gene            1284..2177
                     /locus_tag="AC16FV_12335"
     CDS             1284..2177
                     /locus_tag="AC16FV_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850872.1"
                     /translation="MSLQGPLIVVAEQPAAELVEALSAAGAFPIVETTWADAPAAFLT
                     VKPSAVVIAEPGSPANDASVRMFCLQIATVNGPIVPVIARVRGEEDAAVPIGLPADAA
                     LPVERLLVRLQSAMRVRALHATMLRRIELFASHGGRLPMLPVGDALDDATVLIAGRGP
                     LYPMLSVAMGERVRVAGALSVEAAARHLNARDIDGIVVGDGFSSRMVEAFLTVLAQEP
                     RFRDIPVALIGEAPPDFAEALPNIDQVAVDPERLVTRMVPLVRMRAFEARLSACSSRS
                     TLRVCSTPRPVCSRASHSGAI"
     gene            2123..2548
                     /locus_tag="AC16FV_12340"
     CDS             2123..2548
                     /locus_tag="AC16FV_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850873.1"
                     /translation="MFDPETGLLTRESFRRDLSEVMVGASDRSMPLSVARFSFDGATD
                     ECTGLDAARLMTRLIRNIDFATRDEDGAILVVFTQTDLRSTHVIVRRIAGTLKDTMLT
                     PRRTAEPLTANVTLATLKAGDTLDTLMMRVMGSRMVAAQ"
     gene            2585..>3118
                     /locus_tag="AC16FV_12345"
     CDS             2585..>3118
                     /locus_tag="AC16FV_12345"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR2850874.1"
                     /translation="MSSQQPVRIDVVSDVVCPWCFIGKRRLEKAIALIPDTPVEVHWR
                     PYFLNDWIPREGISRQQYLTTKFGSAERYKAIAQRVSAAAAAEGLTYAMDKISRQPNT
                     LDAHRLIRWAGVIGKAGEMKQRLMDLYFTEGADLTNHAVLVQAAADVGLDAEDIREAL
                     ASDKDVAEVEQEAISAKD"
BASE COUNT          530 a          976 c          956 g          656 t
ORIGIN      
        1 gatccctcta ctgtggcgcc agcaggacat tgtagaggca ctccgccgtg tcgacgcaaa
       61 agccatcgta acccttccgc gtatcggaac gaccgcgtat gccgagttgg cgatgcaagc
      121 ggcggtcgag ttgtttccga tccgccatgt ctgcagcttc ggcgctaatt tacctgacgg
      181 tatcgtgtct ctcgatgagg cgttcgcctc cggtagcgtc gatgtcgcgg caccctacat
      241 caggccaggt ccggctgctg cgcatgttgc ggccattact tttggcctgg atgccaaagg
      301 attggtagaa ctcgtcgccg ccggcctcga gacgtttctg gaggccggcg cagaagctga
      361 cacgtcgctg ctctcgacga ttccaatcgg ttcttttgca ggcctcgccc tcaccctggt
      421 gtattggctg ctgtcgggcg gcgcgctgca tctgcatcac ggtttcgatc cagatgcctt
      481 cgacgcgcaa tctggctctt taacgggcgg gacagtcgtg ttgcctgcgt ccgcgcttgc
      541 atcggtcgct gaggcaggcc tgctccggga ggcgcaccgc acgatcgtcg cgctctggcg
      601 tgcgcctgaa cggctgacgt cagccaaacc atggcatggc aaaccgacgc tcgtggacgt
      661 ggcgagcttt ggcgagatcg gtctcgtcgc cgcgcgtcgt ggtgaaaatc gattgccgac
      721 cgctatccgg cacggcgttg tcaattcatc acaccgcgct ccaggcgcgc ctgtcgtgat
      781 cgagaccgcc cgcagcgacg ttggcacgct ggtgttgcgg gggcgcatgg tttcggttca
      841 tgccttcgct ccgggtgcca agagtgctta tcccccacgc ctgtcgtctg acagcgcggg
      901 atatgttgat accggatttg cttgccgcct cgacagaaac gctcaaacgc tgaccatcac
      961 tgcgccgccg tccggcacca tagctgtcgg cggctactgt ttccgtgcaa acctaatcga
     1021 tgccttgatc gctgatgccg aactggatgc gacgcttgtc gcattgcccg atagcgatct
     1081 tggccagcgc tttgctgggg ccgcggctga ccgcgcgacc cttcgcgcca aattgcaggc
     1141 gcaaggtatc aatccgctga tatcgggggc atttgtgctg cgtggcgctc ctgaagctgc
     1201 gtaatttttg gcttgtcgtt gacgtggtat taacagcgct catctagggt cacgcatatt
     1261 ctgtaaacct cgcgtgcgct ctgatgtcgc ttcaaggtcc gctgatcgtg gtcgccgagc
     1321 aaccggcggc cgagctggtc gaagcgctga gcgcggctgg cgcctttccg atcgtcgaaa
     1381 caacatgggc tgatgcaccg gccgcgttcc tgacggtcaa gccgtccgcc gttgtcattg
     1441 ccgagcctgg ctcgcctgca aacgatgcgt cggtgcgcat gttttgcctg caaatcgcaa
     1501 ccgtcaatgg gccgatcgtt ccggtcatag cgcgcgtgcg cggtgaagaa gatgccgcgg
     1561 tcccgattgg cctgcccgcc gacgctgcgc tgcctgttga gcggctgctc gtgcgcctcc
     1621 aatcagcaat gcgggtgcgc gcgctccacg ccactatgct gcgccgcatt gagctgttcg
     1681 cttctcacgg tggccgattg ccaatgttgc cggttggcga cgcactcgac gatgcaactg
     1741 tactgattgc cggccgtggc ccgctttatc ctatgctcag cgtcgccatg ggggagcgcg
     1801 tcagggttgc cggtgcgctc agcgttgaag cagcagccag gcatctcaat gcccgcgata
     1861 ttgacggcat tgtcgtcggc gatggtttct cttcacgtat ggtcgaggca ttcctcaccg
     1921 tgctggcgca agaacctcgc ttccgtgaca ttcccgttgc gctgattggc gaggctccgc
     1981 cggactttgc cgaagctctg cccaacatcg atcaggtcgc cgttgatcca gagcggctag
     2041 tcacgaggat ggtacctctg gtgcgcatgc gcgcgttcga ggcccgactc agcgcatgct
     2101 caagtcgctc gacactgagg gtatgttcga ccccgagacc ggtttgctca cgcgcgagtc
     2161 attccggcgc gatttgagcg aggtgatggt aggagcttcc gaccgcagca tgcctctgtc
     2221 cgtcgcgcgc ttttccttcg acggcgcaac tgacgagtgc acaggcctcg atgctgcgcg
     2281 gctcatgacg cgcctaatcc gcaatattga cttcgcgacc cgcgatgagg acggcgcgat
     2341 cctggtcgtc ttcacccaaa cggatttgcg cagtacccac gtgattgtcc gccgcatcgc
     2401 gggcactctc aaggacacca tgctgacgcc ccggcggact gccgaaccgc tcaccgccaa
     2461 cgtgacgctc gcaacgctga aggccggcga cacgctcgat acgctcatga tgcgggtgat
     2521 gggcagccgc atggttgcgg cgcagtagac ggcgttttag gctggccccg aagaggagcc
     2581 agccatgtcc agtcaacaac ccgtccgcat cgatgtcgtt tccgatgtcg tttgtccgtg
     2641 gtgcttcatc ggcaagcgcc ggctggaaaa agcgatcgca ctcattccgg acaccccagt
     2701 cgaggtgcac tggcgtccct atttcctcaa cgactggatt ccgcgcgagg ggatttcgcg
     2761 ccaacaatac ctgaccacca aattcggttc ggctgagcgc tataaggcca tagcacaacg
     2821 cgtgtcggcc gcagctgcgg ccgaaggcct cacgtatgcc atggacaaga tcagccgaca
     2881 gcccaacacg ctcgacgcgc atcggttaat ccgctgggcg ggagtcatcg gcaaggcggg
     2941 tgagatgaaa cagcggctga tggatctcta tttcaccgag ggcgccgacc tgaccaacca
     3001 tgccgtgttg gtgcaggctg ctgccgatgt tggtctggac gctgaggaca tccgcgaagc
     3061 cctcgccagt gacaaggatg tcgccgaggt cgagcaggaa gcgatttccg ccaaagac
//
LOCUS       DBNGZE010000477         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_969678, whole genome shotgun sequence.
ACCESSION   DBNGZE010000477 DBNGZE010000000
VERSION     DBNGZE010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_969678"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..>1588)
                     /locus_tag="AC16FV_12350"
     CDS             complement(52..>1588)
                     /locus_tag="AC16FV_12350"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR2850875.1"
                     /translation="LFPELALTGYPPEDLLLRPDLSVRVELALGEMLRSISGIDVIVG
                     YPKRQYGKLFNAASLLRGGRVTATYFKHCLPNYGVFDEKRYFAEGTDPCVLDIKGARI
                     GITVCEDVWERGPVEQAVEAGAKLMFNLNASPFHKNKGKERFEVVARRARNNGVPVVY
                     LNLVGGQDELVFDGDSFVVDARGEVTQRAPAFVEGLYLAEIDTDSQAPVSAQIMPELS
                     EEESVYSALVLGVRDYVGKNDFAGVVIGLSGGIDSALTLCVAVDAIGASRVEAVMMPS
                     PFTAPMSLEDARVQALTLGVKYSFIPIDRLFEAFREALKEEFQGRKPDTTEENIQARI
                     RGMLLMAISNKTGKMVLTTGNKSEMAVGYATLYGDMAGGFAAIKDVPKTLVYRLAEYR
                     NGIKPVIPRRVFERPPSAELAPDQKDTDSLPPYPMLDPILERYVELDQSAEDIIAAGF
                     DEATVRRVLRMVDRNEYKRRQAAPGVRITPRAFGRDRRYPVTSGYGRGPDKPGGNGPA
                     SGI"
BASE COUNT          308 a          514 c          474 g          292 t
ORIGIN      
        1 gcgaccctgg cgtcctggca gttcaaaatc atatttgttc cgttggagaa tctaaatacc
       61 actcgccggg ccgttgccac cgggcttgtc cgggccgcgg ccgtagccgg aagtaacggg
      121 gtagcgccga tcgcgcccga aggcgcgcgg cgtgatgcgc acgccgggcg ccgcctgccg
      181 gcgcttgtat tcgttgcggt ccaccatgcg cagcacccgg cgtaccgtag cttcgtcgaa
      241 accggcggcg ataatgtcct ccgccgactg atccagttcg acatagcgtt ccaggatcgg
      301 atcgagcatt gggtacggcg gcagcgaatc cgtgtccttc tggtccggag cgagctccgc
      361 cgagggtggg cgctcgaaca cgcgccgggg aatgaccggc ttgatcccgt tacggtactc
      421 cgcaagacga taaaccagtg tcttgggcac gtccttgatg gcggcaaagc cgccggccat
      481 gtcgccgtag agcgtggcat agcccaccgc catctcgctc ttgttgccgg tggtcagcac
      541 cattttgccg gtcttgttcg agatcgccat gagcaacatg ccgcggatgc gtgcctggat
      601 gttttcctcg gtggtatccg gtttcctgcc ctggaattct tccttgagcg cttcacgaaa
      661 cgcctcgaac aggcgatcga tcgggatgaa gctgtatttc acgcccagcg tcagggcctg
      721 tacccgcgcg tcctcgagac tcatcggcgc cgtgaaggga gaaggcatca tcaccgcctc
      781 gactcgcgac gcgccaatgg catcgaccgc gacacagagg gtaagcgccg aatcgatgcc
      841 gccggaaagg ccgataacca caccggcaaa atcgttcttg ccgacgtaat cgcgcacacc
      901 cagcaccagc gcggaatata cgctttcctc ctcggaaagc tccggcatga tctgagcgga
      961 aacgggcgcc tggctgtcgg tgtcgatttc agccagatac aacccttcaa cgaacgccgg
     1021 ggcacgctgg gtcacttcgc cacgcgcgtc caccacgaac gaatcgccgt cgaacaccag
     1081 ctcgtcctgc ccgccgacca gattcagata gacaacgggc acgccattat tgcgcgcgcg
     1141 gcgggcgacg acctcgaacc gctctttccc tttgttcttg tgaaatggcg aggcgttaag
     1201 attgaacatg agcttcgcgc ctgcctcgac tgcctgctcc accggtccgc gctcccacac
     1261 gtcctcgcag acggtgatgc cgatgcgcgc gcccttgata tccagcacgc agggatcggt
     1321 gccctccgcg aaataacgct tctcgtcgaa cacgccgtaa ttgggaagac agtgcttgaa
     1381 ataggtcgcg gtcacccggc cgccgcgcaa cagcgaggca gcgttgaaca gtttcccgta
     1441 ttgccgcttg ggatagccga caatcacatc aatgccggaa atgctccgca gcatctcgcc
     1501 cagcgcgagc tcgacgcgca cgctgaggtc aggacgcaac agcagatcct cgggcgggta
     1561 accggtgagc gcgagttcgg gaaagaga
//
LOCUS       DBNGZE010000478         3045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_900684, whole genome shotgun sequence.
ACCESSION   DBNGZE010000478 DBNGZE010000000
VERSION     DBNGZE010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_900684"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..885
                     /locus_tag="AC16FV_12355"
     CDS             142..885
                     /locus_tag="AC16FV_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850876.1"
                     /translation="MNVGLESFRWEESSGGQRLLRETGPRLTAGITLDHLMHGDLSRP
                     YAMEVRGYAGFIDYDGQTQAGVSAKTDVDYFGVSGEIMGGMRLAGSNRVDLLGGLGVD
                     TWTRDLQDGVAANGSAALGYQEDYFILYGKLGPGFLFQNGATRSYLQFGIKYPFYTYE
                     RVYLSTIGFDSDVDLRPGKKISGFAKWRMSWGGGEPGKPQLGMSFYYDSFRFSESDSK
                     LVTGGGVLYSVHQPESHMDVLGARLEYYF"
     gene            complement(1092..1331)
                     /locus_tag="AC16FV_12360"
     CDS             complement(1092..1331)
                     /locus_tag="AC16FV_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850877.1"
                     /translation="MNTHRITAMAMAVALAGLAHTLAAEENISTTTVNENFQQTAAEQ
                     KSDTENTVSEMDMPAQTVSQTSDNLLSELKVYPSF"
     gene            1682..2875
                     /locus_tag="AC16FV_12365"
     CDS             1682..2875
                     /locus_tag="AC16FV_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR2850878.1"
                     /translation="MNISVIVLLGVFVLIAVRRVGNFHFGLWQIMLLGAVAVLVTGQI
                     GLAQALRAIDLDVMLFLFGVFVVGQALEESGYLFHLSYKLFSRARTADRLILLLLFGV
                     GMASAFLMNDTLAIISTPLVLLLAKQHRMSPRVLLLALAFAVTLGSVMSPIGNPQNLL
                     ISLHGRMDNPFVEFFGYLALPTAINLLAAFFILKFFYHESFHGEALVHTRQEMRDPRL
                     ARLARLSLILMILLVFLKIALVFTLPGFDFRLTWIALIAAAPILLFSAKRFSILRHSD
                     WRTLVFFAAMFVLMESVWNSGLFQSLMARWNPDISTTGVILPVSVLLSQLISNVPLVA
                     LYLPMLMHTGAATPELLALAASNVIIIQNAERKTGHTLGFVEFARVGIPLTLVNALVY
                     WIFLT"
BASE COUNT          554 a          863 c          909 g          719 t
ORIGIN      
        1 cgatctcttt cctagcttac tcacgttaag tgaattgatc agacccacat gcgcaaatat
       61 ttcgagacag tcctgatatt ttgcgtgatt tgcgcacccc ttgtgcatgc tgacgaggtg
      121 atcccaaagc catcgctcac cgtgaatgtg ggcctggaaa gcttccgctg ggaggaatcc
      181 agcggcggtc agcgtctgct gcgtgaaacc ggtccacgac tgacagcggg catcacgctg
      241 gatcatctga tgcacggcga tctcagcaga ccttatgcga tggaagtccg cggctatgcg
      301 ggattcattg attatgacgg gcagacccag gcaggcgttt cggccaaaac cgacgtcgat
      361 tatttcggcg tcagcggcga gatcatgggc ggcatgcgtc tggcaggaag caatcgcgtg
      421 gatctgctcg gtggactggg agtggatacc tggacccgtg atcttcagga tggtgtcgcg
      481 gccaatggat cggccgcgct gggttaccag gaggattatt tcattctcta cggcaaactg
      541 ggaccgggat ttctttttca gaacggtgca acgcgatctt atttacagtt cgggatcaaa
      601 tatccgtttt acacttatga acgggtatac ctctcgacca tcggtttcga cagcgatgta
      661 gacttgagac ccggcaagaa gatttcaggc tttgcaaagt ggcggatgag ctggggcggc
      721 ggcgagccgg gcaaaccgca acttggcatg agtttctatt acgacagctt ccgtttcagt
      781 gagtcagact caaagctggt cacgggagga ggcgtcctgt acagcgtgca tcagcctgaa
      841 agccacatgg acgtgctggg cgcgaggctc gaatattact tctagaagct gtctcaaaaa
      901 ctgctgcgtg ctgcgctcct ttcccgctgc gctatccggc tccgcgggaa agtccagggc
      961 cgccatcctt ggcggcccgt tcggcgacac gaacgtccac cggacgttcg tgaaatctcc
     1021 gccttaccct cacccggctg acggccgctc gcgacgtttt tgagatagct tctgtctgac
     1081 ttgagatcga atcagaacga cgggtaaacc ttcagctcgc taagcagatt gtcggatgtc
     1141 tgtgaaacgg tctgtgctgg catgtccatc tccgacacgg tattttccgt atccgatttc
     1201 tgctcggcgg cagtctgctg aaagttttcg ttgacggtgg ttgtggagat attttcttcc
     1261 gcggctagtg tatgcgccaa accggcgagc gccacagcca ttgccatggc ggtaatgcga
     1321 tgcgtgttca tgattccttt ccgtccctgc aatcgaaaaa gtcccgcgtc acagtgtaaa
     1381 accataatgc ctgcaagccc cgcggaacag gggtggcatg acgaaagcga tggtattact
     1441 gaccggcggc cggtctcgga tcgtctggct cgggggagtt cagccggtcc ttggctcgaa
     1501 gcatgccgct gttgttattt ttagcatccg tcagtggttt tgtccaaaaa aagtgggcgg
     1561 gcttgccgca ggagcgaggg ccgtgggtcg cttcccggcc agcggttgtc caccgggtgt
     1621 ttttcaatat gatgggcgcc ggctacccca cccaaagaac ccggccattg cggcacaagg
     1681 catgaacatt tccgtcatcg tcctgctcgg cgttttcgtg ctgatcgccg tgcgccgcgt
     1741 tggtaatttc cacttcgggc tgtggcagat catgctgctc ggcgccgtgg cggtactggt
     1801 cacgggccag atcggcctcg cgcaggcgct gcgcgccatc gatctggatg tcatgctgtt
     1861 cctcttcgga gtgttcgtcg tcgggcaggc gctggaggag agcggctacc tgtttcatct
     1921 ttcttataaa ctgtttagcc gcgcgcggac ggcggatcgc ctgattctgc tgctcctgtt
     1981 cggtgtcggt atggcctcgg ccttcctgat gaacgacacg cttgcgatca tcagcacgcc
     2041 gctggtgctg ttgctggcca aacaacaccg catgtcgccc agggtcctgc tgctggcgct
     2101 ggccttcgcc gtgacgctgg gcagtgtcat gagcccgatc ggcaacccgc aaaacctgct
     2161 gatatccctg cacggccgga tggacaaccc cttcgtcgag tttttcgggt atctggcgct
     2221 gccgacggcc attaacctgc tggcggcttt ttttattctc aagtttttct accacgagag
     2281 cttccacggc gaggcgctgg ttcacacacg acaggagatg cgagatccga gactggcgcg
     2341 gctggcacgc ttgtcgctga tcctgatgat cctgctcgtt tttctcaaga tagcgctggt
     2401 attcacattg ccggggttcg atttccggct gacctggatc gccctgatcg ccgccgcgcc
     2461 gatcctgctg ttcagcgcga aacggttttc aatcctgcgt catagcgact ggcgcacgct
     2521 ggtttttttc gcggccatgt tcgtgctgat ggagagcgtc tggaattccg gtttgttcca
     2581 gtcgctgatg gcgcgatgga atccggatat ttccacgacg ggcgtcattc tcccggtcag
     2641 cgtgttgttg agccaactga tttccaatgt tccgctggtg gcgctttatt tgccgatgct
     2701 catgcacacc ggcgccgcga cgccggaatt gctcgcgctc gcggccagca acgtcattat
     2761 catccagaat gccgagcgca aaaccggcca caccctgggt tttgtcgaat tcgcgcgtgt
     2821 agggattccg ctaacgctgg tgaacgcact ggtctactgg atttttctga cgtaatgcag
     2881 gctggtggcc cgggatcagg ccgggccgga ggagcgcagg cgccggcgag tgagcgccag
     2941 catgtcgaag atcagctcgg ccagcgcgtt gaaccgcgcc acccgcaata tcagtcctgc
     3001 cgcggtaagg cggcgcacct cgcgcacctc gcgcaaggcc ggcgc
//
LOCUS       DBNGZE010000479         2403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1386684, whole genome shotgun sequence.
ACCESSION   DBNGZE010000479 DBNGZE010000000
VERSION     DBNGZE010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1386684"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..>2403)
                     /locus_tag="AC16FV_12370"
     CDS             complement(216..>2403)
                     /locus_tag="AC16FV_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2850879.1"
                     /translation="AAVDVQGGSVREILVMPDQRRLAGYGLSFEDLLQAIRKNPEVDS
                     RVSQLPLKKRRTRREPMLSGSLAAVAAIPVALPDGESIRLSEVAGLTLNQEANPVSSR
                     ADGAKVIKVTVHKQAQAALSDVVAQVRSQVDWMRANGLIPDGIEVQLVSGRFDEARQP
                     LRKMAYAFLIGFILVLSAAQLLWGRRRRTLILGVIVAATLQGVFIAMALSGMALDIMT
                     LGGLVLGTGLFGGSVMLMFEKTAQPVPAPVSRVSPVMAAAMLLVAALTPAWFAGGELG
                     ALYREFIAVFVGAWLLAALLAWWLVPMFDTRLREKAPWNAAVSHALARMRQSYDGLLH
                     RLLPRAGLALLVAAVTLAGLAAVVFTKLGNTSMPDIPSGQEINLRIEGSNGADLLALA
                     NDITQRLRSLTELRQVSHSAQVSREEWVLHMDEARAQELGVDIAMAGKALAIALNGIP
                     AGGFRDADHHYNVRMRLPSEDAGSVATGKILLLGELEQRPAVYLRDVATLERVVVPTQ
                     IRRHNGKPMIELTARMADESLPAQTMRKVQATVANIKLPSGCQLLFGRSERTAPEGRG
                     LMALAISVVLIVITATWLFRSLRPALLIALTAGATLIGTGVVLLLSGMTLSAPVWLGA
                     LLLQGISAGHAGAWVAQCEAQQPEVTLPQRLRQTARHLPGTLFAMTLTAILGMLSLMW
                     VNGGASVLHTLIIVLAIGLLVSLLINLLLTPLLYSLISRKEQTPMS"
BASE COUNT          536 a          794 c          669 g          404 t
ORIGIN      
        1 cgtcgagctt cttggcgctg aaggcgtcct tgggccattc ggcggccagg gcccagccgg
       61 ggcggaacag gctggcgccg acaccgacca gcacggcggt ggcgagcgtg tgcttgagga
      121 agagacgacg gggaaggtgg gtcacggggt cctccggtaa atcaaaaaac actgatatga
      181 aatgttaaca gacatcggca tgtctcccat agacgctaag acatgggagt ttgttccttg
      241 cgcgagatca gcgaatacaa caaaggagtc aacaatagat taatcaacag cgaaaccagc
      301 aagccgatgg ccagaacaat aataagtgta tgcaggacag atgcgccgcc attgacccac
      361 atcaatgaca gcatgcccag aatggccgtt agtgtcatgg caaacaaggt ccccggaaga
      421 tgccgggcag tttgtctcaa ccgctgcggc aaagtcactt ccggttgttg tgcttcgcat
      481 tgcgccaccc atgcgccggc atgtcccgcc gagataccct gcaacaacag cgcacccaac
      541 cataccggtg ccgacaaggt catgccggat aacagcagca caacgcccgt cccgatcaac
      601 gttgcgcctg ccgtcagcgc tatcaacagc gccggacgca gcgaacgaaa aagccacgtc
      661 gccgtgataa cgatcagcac aaccgatatc gccaatgcca tcaacccccg gccctccggc
      721 gcggttcttt cactgcgtcc gaataaaagt tggcaaccgg atggcaattt tatattggcg
      781 accgtcgcct gtacttttct catcgtctga gccggcaagg actcatccgc catccgggcg
      841 gtaagctcga tcatgggttt cccgttgtga cgacgaatct gcgtcggcac gacaacccgc
      901 tccagcgtgg caacatcgcg caaatacacc gccgggcgct gctcgagttc gccaagcagc
      961 agaatcttgc ccgtggcgac gctgcccgcg tcttccgacg gcagccgcat gcgcacgtta
     1021 tagtgatgat ccgcatcgcg aaaacctccc gccgggattc cattcagcgc aatggccaac
     1081 gccttgccgg ccatggcgat gtccacgcca agctcctgtg cgcgcgcctc gtccatgtgc
     1141 agcacccatt cctcacggga gacttgcgcc gaatggctca cttggcgcag ctccgtcagc
     1201 gaacgcaagc gttgcgtgat gtcattggca agcgccaata aatcagcgcc gttcgatccc
     1261 tcgatacgca ggttgatttc ttgcccggac ggtatatccg gcatggaagt attcccgagt
     1321 ttcgtgaaaa caacggccgc caatcccgcc agcgtcaccg cggccacaag cagcgccaat
     1381 ccggccctcg gcaacagacg gtgcaacaaa ccatcatacg actggcgcat gcgcgccaag
     1441 gcatgactga cggcggcatt ccacggtgct ttttcccgga gacgcgtatc gaacatcggc
     1501 accagccacc atgccagcag cgcggccagc aaccaggcgc cgacaaatac ggcgatgaac
     1561 tcgcggtaca gcgcacccag ttcgccgccg gcgaaccacg ccggggtcag cgccgccacc
     1621 aggagcatgg cagcggccat aacaggactg acacggctga ctggcgccgg cacaggctgc
     1681 gcggtttttt caaacatcag catgacactg ccgccgaaaa gacccgtgcc cagcaccaga
     1741 ccgcccagcg tcatgatatc gagcgccatg ccggacaagg ccatcgcgat gaaaacgccc
     1801 tgcagcgtgg ccgcgacaat cactccaagt atcagcgtgc gccgcctgcg gccccatagc
     1861 agttgcgccg cggacagcac cagaataaaa ccgatgagaa acgcgtaagc catcttccgc
     1921 agaggctgcc gtgcctcgtc gaaacgcccc gaaaccaact gaacctcgat gccatcgggt
     1981 atcagcccgt tggcgcgcat ccagtccacc tgcgagcgga cttgcgcgac cacgtccgac
     2041 aaggcagctt gtgcctgttt gtgcaccgtc actttgatga ccttcgcgcc gtcggcgcgc
     2101 gacgagaccg gattcgcttc ctggttcagc gtcagcccgg ccacttccga gaggcgaatg
     2161 ctttcgccgt caggcaaggc cacgggaatc gccgccacgg cggccaggct gcccgacagc
     2221 atgggttcac gtcgtgttcg cctcttcttc aggggcaact ggctgacgcg ggaatccacc
     2281 tcgggatttt tccggattgc ctgcaacaga tcctcgaacg aaagaccata tccggccagc
     2341 cgtcgctgat ccggcatgac caggatttcg cgcacgctgc cgccctgaac atccaccgcg
     2401 gcg
//
LOCUS       DBNGZE010000480         3907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_901055, whole genome shotgun sequence.
ACCESSION   DBNGZE010000480 DBNGZE010000000
VERSION     DBNGZE010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_901055"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="AC16FV_12375"
     CDS             <1..1046
                     /locus_tag="AC16FV_12375"
                     /inference="COORDINATES: protein motif:HMM:NF027219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HIR2850880.1"
                     /translation="KPRRKKASRRLVTMSWTRKSDENLLDTRLCDLGLTLNGSKIQKE
                     VELLWHDLANRGFAFRPHCWISDCWFCPDGVPGFAIPFYLVHPRLMRLEKAHMLEFDG
                     NTFESRMKLLRHETAHALANAYRLHLKQSWRRRFGRASKAYPESYLPRPYSRNHVLHL
                     DNWYAQSHPAEDWAETFAVWLDPGSRWREKYRDWPALKKLNYVDTLMKEIVGHTPPVR
                     SKKQVDSINTLRITLREYFAEKQTRLQQDLPRFHEEQLRRIFPASANLKNERAAHFIR
                     RLRREAIATVAHWTSESRYRINLTIEDMIHRCEDMNLHASRDKEETKIALISSLVMLY
                     MGSLRSGMSRLAI"
     gene            1043..2062
                     /locus_tag="AC16FV_12380"
     CDS             1043..2062
                     /locus_tag="AC16FV_12380"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR2850881.1"
                     /translation="MKKLRVMMVVHYTLVPPDDLHDKNDPRMEKFRTEYDVKTALLNI
                     GHEVQVVGVYDDLAPIRKVIEEWKPHIAFNLLEDFAGNSAFDYYVVSYFKMMRIPHTG
                     CNPRGLLLARDKALTKMILTYHRISVPDFDVFPLRKKISQRRKFTYPLIVKSLTEEGS
                     VGIAKASYVENEAQLRERVALIHEKLNGDAIAEQYIEGRELYVTVLGNTKLEVLPIRE
                     LVFGNSEPGTPKMATYKVKWDDKYRERWGIEYQFARNLPNGMEERIGHLCKRVYHLLD
                     MDGYGRIDLRMAADGELYVLEANPNPGIARDEDCTQSAIKAGMSYEDFIQRILHLGLT
                     ARSIE"
     gene            2257..2712
                     /locus_tag="AC16FV_12385"
     CDS             2257..2712
                     /locus_tag="AC16FV_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding protein SH3"
                     /protein_id="HIR2850882.1"
                     /translation="MAELINGFPSDGVVTINRVLLKPRYTVDDLQERVAVLCENVKTY
                     HSDTGFIGGFVALNSGAISNEGSTIGQAVKSPLKDKEALIVTFWRSFEEHEQSHRSDT
                     FQPLFKQVLELCENGNEEIAYTMLWSGRAYSPEEAKAARKAKAKYGKAA"
     gene            2836..>3907
                     /locus_tag="AC16FV_12390"
     CDS             2836..>3907
                     /locus_tag="AC16FV_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR2850883.1"
                     /translation="MSQRPYVVPDSARRERLSRLESLLQERILILDGAMGTMIQRYKL
                     GEKEYRGERFASWAHELKGNNDLLTLTQPKIIRDIHAAYLDAGADILETNTFNSQSIS
                     LADYRMEELAYELNREGARLARTVADEFEKKNPHKPRFVAGVLGPTNRTASISPDVNN
                     PGFRNVTFDQLVAAYTDSLHGLIDGGSDIILVETIFDTLNAKAALFAIDQYFENHGLR
                     LPIMISGTITDASGRTLSGQTTEAFWNSLSHANPLSIGLNCALGVTQLRQYVEEMASV
                     ANVHVSSHPNAGLPNEFGGYDESPEFMAKHVREWAESSFLNIVGGCCGTTPPHIAAIA
                     KAVGDVRPRSLPKIEPACRLSGL"
BASE COUNT          848 a         1259 c         1114 g          686 t
ORIGIN      
        1 gcaagccgcg ccggaaaaaa gcatcgcgcc ggctggtaac gatgtcctgg acgcgcaagt
       61 ccgacgagaa tctgctcgac acgcgcttgt gcgacttggg actcacgctc aacggcagca
      121 aaatccagaa agaggtcgag ctgttgtggc acgacctcgc caatcgcggc tttgccttcc
      181 gcccgcattg ctggatatcg gattgctggt tctgccccga cggcgtaccc ggcttcgcga
      241 ttccgttcta tctcgtgcac ccgcggctca tgcgcctgga aaaggcgcac atgctcgaat
      301 tcgacggcaa caccttcgag tcgcgcatga aactgttgcg gcacgagacc gcgcacgcgc
      361 tggccaacgc ctaccgcctg cacctgaaac agagctggcg ccgccgcttc gggcgcgcca
      421 gcaaggccta tcccgaaagc tatctgccgc ggccctacag ccgcaaccat gtgctgcacc
      481 tcgacaactg gtatgcgcag agccacccgg ccgaggactg ggccgaaacc ttcgctgtgt
      541 ggctcgaccc gggctcgcgc tggcgcgaga aataccgcga ctggccggcg ctgaagaaac
      601 tcaattacgt ggatacgctg atgaaggaga tcgtcggcca cacgccgccg gtacgcagca
      661 agaaacaggt ggattcgatc aacacgctgc gcatcaccct gcgcgaatac ttcgcggaaa
      721 aacagacgcg cctgcaacag gacctgccgc gcttccacga agagcaactg cgccggatct
      781 ttcccgcctc ggcgaatctc aagaacgaac gcgcggcgca tttcatccgg cggctgcgtc
      841 gcgaagccat cgccaccgtg gcccactgga cctccgagtc gcgctatcgc atcaacctca
      901 ccatcgaaga catgatccac cgctgtgagg acatgaatct tcacgcctcg cgcgacaagg
      961 aagaaaccaa gatcgcgctc atctcctcgc tcgtcatgct ctatatgggt tcgctgcgca
     1021 gcgggatgtc gcgcctcgcg atatgaaaaa gctgcgcgtc atgatggtgg tgcactacac
     1081 gctggtgcca ccggacgacc tgcacgacaa gaacgacccg cggatggaga aattccgcac
     1141 cgagtacgac gtcaagaccg cgctgctgaa catcggccat gaagtgcagg tcgtgggcgt
     1201 gtacgacgac ctggcgccga tccgcaaggt catcgaggaa tggaaaccgc acattgcctt
     1261 caatctgttg gaggacttcg ccggcaacag cgccttcgac tattacgtcg tcagctattt
     1321 caagatgatg cgcatccctc acaccggctg taatccgcgc ggcctgctgc tggcgcgcga
     1381 caaggcgctg accaagatga tcctcaccta ccaccgcatc agcgtgccgg acttcgacgt
     1441 ctttccgctg cggaagaaaa tcagccagcg acgcaagttc acctacccgt tgatcgtcaa
     1501 atcgctgacg gaggaaggct cggtcggcat cgccaaggcc tcgtacgtgg agaatgaggc
     1561 ccagctgcgc gagcgcgtcg cgctgattca cgaaaagctc aatggcgatg ccatcgccga
     1621 acagtacatc gaagggcgcg agctttacgt caccgtcctc ggcaatacca agctggaagt
     1681 cctgcccatt cgcgagctgg tgttcggcaa cagcgaaccg ggcacgccga agatggcgac
     1741 ctacaaggtg aagtgggacg acaaataccg cgagcgttgg ggcatcgaat atcagttcgc
     1801 gcgcaacctg ccgaacggga tggaggaacg catcggccac ctgtgcaagc gcgtctacca
     1861 cctgctcgac atggacggct atggccgcat cgacctgcgc atggcggcgg acggcgagct
     1921 ttacgtgctg gaggcgaatc ccaaccccgg catcgcgcgc gacgaggact gcacccagtc
     1981 cgccatcaag gccgggatga gctacgaaga cttcattcag cgcattctcc atctgggact
     2041 gacagcccgc tccatcgagt aaccccgacg gggtattccg atccccaagt cgggatatgt
     2101 cccgcataaa tggcctgcct cttgcactct tgaaaccctg atttttgtcc ctatattgtt
     2161 gactgtttga gtaaggaatt ggtaaaaggg gatttggtta atctgaaaca aaactcggcc
     2221 gaaaggccgt gactttgtga aacggaggta attgttatgg cagaactcat caacggcttt
     2281 ccttccgacg gcgtggtcac gatcaaccgc gtactgctca aaccccggta caccgtggac
     2341 gacctgcagg aacgcgtcgc ggtattgtgc gagaacgtga agacctatca ctccgacacc
     2401 ggcttcatcg gcggcttcgt ggcgctgaac agcggcgcca tttccaacga aggctcgacc
     2461 atcggccagg cggtgaaaag cccgctcaag gacaaggaag cactgatcgt cacgttctgg
     2521 cgttccttcg aggaacatga acagtcgcat cgcagcgata ccttccagcc gctgttcaaa
     2581 caggtgctgg agctgtgcga gaacggcaac gaggaaatcg cctacaccat gctgtggtct
     2641 ggccgtgcct attcccccga ggaggccaag gccgcacgca aggccaaagc caagtacggc
     2701 aaggccgcgt aaagccgtcc tgttttcttg ccgttgcaac accccgccag gcgcggggtg
     2761 ttgtgttttt aggcattcgc cacggctttc cggtaaactc cctcagctct taacagagcc
     2821 agaagctgaa gaagaatgtc ccaacgtccc tacgtcgttc ccgacagcgc ccggcgcgaa
     2881 cgcctgtccc ggcttgagag cctcctccaa gagcgaatcc tgatcctgga cggtgccatg
     2941 ggcaccatga tccagcgcta caagctgggc gaaaaagaat atcgcggcga gcgcttcgcg
     3001 agctgggcac atgagctgaa gggcaacaac gacctgctaa cgctgacgca gccgaagatc
     3061 atccgcgaca tccacgccgc ctatctcgac gccggcgccg atatcctcga gaccaacacc
     3121 ttcaactcgc agtccatctc cctggcggat tacaggatgg aggagctggc gtatgagctg
     3181 aatcgcgaag gcgcgcgtct ggcacgcacc gtggccgacg aattcgagaa aaagaatccg
     3241 cacaagccgc gcttcgtcgc cggcgtgctg gggccgacca accgcaccgc ctcgatctcg
     3301 ccggacgtga acaacccggg ctttcgtaac gtcacgttcg atcagctggt cgcggcctat
     3361 accgactcgc tgcacgggct gatcgacggt ggctccgaca tcatcctggt cgaaaccatt
     3421 ttcgacaccc tgaacgccaa ggccgcgctc ttcgccatcg atcaatactt cgagaaccat
     3481 ggcctgcgtc tgccgatcat gatctccggc acgatcacgg atgcctcggg ccgcaccctc
     3541 tccggccaga cgaccgaagc attctggaat tcgctgtccc acgccaatcc gctcagtatc
     3601 ggtctcaatt gcgcgctggg agtgacccaa ttgcgccagt acgtggaaga gatggcgagc
     3661 gtcgccaacg tgcacgtgtc gagccatccc aacgccggcc tgccgaacga attcggcggt
     3721 tacgacgaat cgcccgaatt catggccaaa cacgtccgcg aatgggcgga gtcgagtttc
     3781 ctcaacatcg tcggcggctg ctgcggcacc acgccgccgc acatcgccgc catcgccaag
     3841 gccgtgggcg acgtccggcc gcgctcgctg ccgaagatcg agccagcctg ccggctgtcc
     3901 ggcctgg
//
LOCUS       DBNGZE010000481         3277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1387054, whole genome shotgun sequence.
ACCESSION   DBNGZE010000481 DBNGZE010000000
VERSION     DBNGZE010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1387054"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(701..1297)
                     /locus_tag="AC16FV_12395"
     CDS             complement(701..1297)
                     /locus_tag="AC16FV_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR2850884.1"
                     /translation="MKQITRTFFTGLAVTLPVVATLYLLYWAALAIERTLDGLLRFVL
                     PKVAYFPGMGVALGVVLVFLVGLLMRTWVARKIFSWTEKLMYRVPLVKTVYGALRDFT
                     EFLSRPKTQGAQQVVLVRLGDTDMRVIGFVTRDDLAGLPPGMGEPGTIMVYLPMSYQV
                     GGYTALVPRASVQPVDMSFEEAMRFTLTAGLSVPAVKG"
     gene            complement(1359..1829)
                     /locus_tag="AC16FV_12400"
     CDS             complement(1359..1829)
                     /locus_tag="AC16FV_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR2850885.1"
                     /translation="MQEQDMEYAGFWVRLGATVIDSLLLIAITLPLVLSFYGWAYFDP
                     EKTGFVAGPADFLINWVLPAVAVILFWKLKQATPGKMILSLRIVDAATGNNPSAGQCV
                     GRYFAYIVSMIPLFLGFIWIAFDKRKQGWHDKLAGTVVVRSKKRGPEPVRFGKT"
     gene            complement(1904..2323)
                     /locus_tag="AC16FV_12405"
     CDS             complement(1904..2323)
                     /locus_tag="AC16FV_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850886.1"
                     /translation="MFMLEYKNIGWWYWLVTAGLLTYGVTVGPAGFLLAIGLTVFQLI
                     HFVIREHGIKAFPVQVRFWYLMLLLVALPEPLQLIYWIPTIGTWAQIIFGYCTMARLV
                     SLLPWNRTEPFTSDLVIKTFFSRPVRGNIKQGFAPAE"
     gene            2597..2824
                     /locus_tag="AC16FV_12410"
     CDS             2597..2824
                     /locus_tag="AC16FV_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR2850887.1"
                     /translation="MRVTSKGQVTIPIEVRQKAGLLPNTEVEFIVRGNTIIVKKAEKT
                     PRRGRRLLTIMRGKATKRLSTDEIMALTRGR"
     gene            2839..3243
                     /locus_tag="AC16FV_12415"
     CDS             2839..3243
                     /locus_tag="AC16FV_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR2850888.1"
                     /translation="MADILVDSNVILDVVTEDPKWYGWSSSQIEKLAESHTLVINPII
                     YREVSIGFDRIEDLDQALPTDFFRRDPLPWDAGFLAGKCFITYRRSGGKRRSPLPDFY
                     IGAHAAVSGIVLLTRDMNRYKTYFPKLALISP"
BASE COUNT          784 a          933 c          882 g          678 t
ORIGIN      
        1 tgccggaaaa tcgcctattt cccgttcgct accgagcgac gcgacgacaa agacgcccgt
       61 tcgccgatcg cgcacacctt ccctacatga aaaccggggt cagatcacag ttttcgctaa
      121 tattaaccag aattgtgatc tgaccccaat tcttcctgct ggatcagggc gataccggat
      181 tggcccagac tgtcgagata gctctccgac aacgaggtac tcgacgaaaa aggaatcggg
      241 gtcagagaac aatggcctga acgcatcccc cgaagattca atgaattaga ggaatacaga
      301 cctgcctttt gaaccgttgg tttgacgcga caagcggact tgcttgatgt tcagccgcca
      361 aaaggcgcaa gatgaccgga caggacaaga atattgggaa ggataaggta aacagaaaag
      421 atgttggtag ggggagagtc atgtatcgac tcgcttgtat ggttgtaggc tggctggcgc
      481 tattgctgtt atcgattctt ttacttcggt gcatctgctt gtcggtccgt gccctttggc
      541 taacttccga gttcgaatca gacgcccggc gcgaccggga tcatgccggt gacggcgttg
      601 gggcaaaacc gttcacgaca ggattggtca cccgccgtat ggccgatgag gagaaggcag
      661 gcggatgatt ccctttccct gagaaaatcg gggtcagatt ctatccctta acggcaggaa
      721 cgctaagccc cgcggtcagg gtaaagcgca tggcttcctc gaacgacatg tccaccggct
      781 gcaccgacgc gcgcgggacc agcgcggtgt agccgcccac ctgatagctc atgggcaggt
      841 acaccatgat cgtaccgggc tcgcccatgc ccggcggcag gccggcgaga tcgtcgcgcg
      901 tgacgaaacc gatgacgcgc atatcggtat cgccaaggcg caccagcacc acctgctgcg
      961 caccctgtgt cttcggccgc gacaggaatt cggtgaagtc gcgcagcgcc ccgtagaccg
     1021 tcttgaccag cggcacgcgg tacatcagtt tctcggtcca ggaaaatatc ttgcgcgcga
     1081 cccaggtgcg catcagcagg cccacgagga acaccagcac cacgcccagc gctaccccca
     1141 tgccggggaa ataggccacc ttcggcaaca cgaagcgcag caaaccgtcc agcgtgcgct
     1201 cgatcgcgag cgccgcccaa tacaacagat agagagtcgc caccaccggc agcgtcacgg
     1261 ccagaccggt gaagaacgtg cgagtgattt gcttcatgga atgtcctccg ctgacggtac
     1321 cgcaggcact tgttcctgca attcgccggg acgatgtgtc aggttttccc gaatcgaact
     1381 ggctcggggc cacgcttctt cgatcttacg acaaccgtcc cggcaagctt gtcgtgccac
     1441 ccctgcttcc ttttgtcgaa ggcgatccag atgaatccga gaaacagcgg aatcatcgaa
     1501 acgatatagg cgaaatacct cccgacacac tgaccggcgg acggattgtt tccggtggct
     1561 gcgtccacga tacgaaggga caagatcatt tttccgggag tggcctgctt cagcttccag
     1621 aacaggatga cggcgaccgc cggcaatacc caattaatca gaaaatccgc cggccccgcg
     1681 acgaatccgg ttttttccgg atcgaagtaa gcccagccgt aaaaggaaag taccagggga
     1741 agcgtgatcg ctatcagcag cagggagtcg ataacggttg cgcccaggcg cacccagaaa
     1801 cccgcgtatt ccatatcctg ttcttgcatc gggaatcctc catgtcgaaa ggctcgataa
     1861 gccgggttgg ctcagaatta tttttacact ccgcttccct ttgttattca gctggggcga
     1921 aaccctgttt gatattcccc ctgaccggac gtgaaaagaa ggttttgatc accagatcgg
     1981 acgtgaacgg ctcggtgcga ttccagggca acaaggatac cagtcgcgcc atcgtgcaat
     2041 atccgaatat tatctgtgcc caggttccta tcgtcggaat ccaataaatc aattgaagcg
     2101 gctccggcag cgccaccaga agcagcatca aataccagaa gcgaacctga accgggaaag
     2161 ccttgatgcc atgttcgcgg atcacaaagt ggatcaattg aaataccgtc aggcctatgg
     2221 ctaacaggaa acctgccggg ccgactgtta cgccataggt aagcagtcct gctgtgacca
     2281 gccagtacca ccagccgatg tttttatatt cgagcatgaa catatcagtt acctccggtt
     2341 tcagttttgg tggttttatg atgcccggta aaccgcttta gatgacgacc atggccaata
     2401 ttagcgaaaa ccatgatctg accccaattc ttggaatact ccgctgacgg taccgccggc
     2461 actttaacag agcgcgccgg ccgcgcccaa gcctggcgcc ggccgagaca ggaaacagtc
     2521 aactgttgtg ttccttgacc cggcttttat aaagtattac tctgtaaagt aagaattcat
     2581 cgtcgaggat gacatcatgc gtgttaccag caagggccaa gtcaccattc ccatcgaagt
     2641 ccggcaaaaa gcagggctac tgccgaatac cgaagttgag ttcatcgtca ggggtaacac
     2701 gatcattgta aagaaagcgg agaaaacacc ccgtcgcggc cgacggctgc tgacgatcat
     2761 gcgcggcaag gccaccaagc gcctcagcac cgatgaaatc atggctctga cccgcggccg
     2821 ctgagaaccg cggcgcccat ggccgatatc ctggtggaca gcaacgtcat tcttgacgtt
     2881 gttaccgaag atccaaaatg gtacggatgg tcttcctcac agatcgagaa gctggctgaa
     2941 agtcatacgc tcgttatcaa cccgattatc taccgcgaag tctccatcgg ttttgaccgg
     3001 atcgaagacc ttgatcaggc cttgccaacg gattttttcc gccgtgatcc gttgccgtgg
     3061 gatgccgggt ttctggcggg gaagtgtttc atcacgtacc gccgttcagg cggcaagcgg
     3121 cgctcgccct tgccggattt ttacattggc gcgcatgccg cggtcagcgg cattgtcttg
     3181 ctgacgcgcg acatgaatcg ctataaaacg tactttccaa aacttgcgct tatatctccc
     3241 tgatgtactt gttgtgagca agaaaaccaa actggcc
//
LOCUS       DBNGZE010000482         4555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_277400, whole genome shotgun sequence.
ACCESSION   DBNGZE010000482 DBNGZE010000000
VERSION     DBNGZE010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_277400"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="AC16FV_12420"
     CDS             complement(<1..702)
                     /locus_tag="AC16FV_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR2850889.1"
                     /translation="MDVSHLMDDLNDVQREAVGAPPGPLLVLAGAGSDKTRVLTHRVA
                     WLVGVEGVSPHSILAVTFTNKAAHEMRGRIESMLHTPVGGMWIGTFHGLSHRLLRSHW
                     KEANLPQTFTILDSEDQLRVITRLMKGMNMDEAYWPPKQAMWFINGHKEEGRRPKDIG
                     ESNDPTQRELRRVYAAYEETCQRSGLVDFAELLLRSYELLRDNESMRAHYQARFRHVL
                     VDEFQDTNRIQYAWLR"
     gene            852..1463
                     /locus_tag="AC16FV_12425"
     CDS             852..1463
                     /locus_tag="AC16FV_12425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2850890.1"
                     /translation="MSLKTKLGEMLAPVFSKREDVLLCKELQEAPGEAPSGRLEVVPV
                     RPEHLKALVQFIHEHHGDVARSLRMLDDCFRNRYEGRVAFLNGQVIGYRWWVTHTMSH
                     PQLKLYHLTLRENEVFAFGLYIARAFRAQGYAGEFLAITQKQLLDLGYKRLYNAVGAS
                     NVPARRLYESFGSKELGLHVSLSFFSLVACCGGRWLRYDPVWM"
     gene            complement(1487..2761)
                     /locus_tag="AC16FV_12430"
     CDS             complement(1487..2761)
                     /locus_tag="AC16FV_12430"
                     /inference="COORDINATES: protein motif:HMM:NF019619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JmjC domain-containing protein"
                     /protein_id="HIR2850891.1"
                     /translation="MYGANNPVLAALLSPKAADEFLTQHWPKRPFVAQANPARWPAVL
                     RCEELASVQNLAKRYRGSLRFTHGRKSDQMIQIDRVDPAILFEMGLTLNFEDIGPYVP
                     GAPEFLRALESELGLNEGSLVMSAFASPYQDGLGCHYDSQDVISIQLLGTKRFHYAPV
                     QEVAMPYGTQYIPGGKPYDEMYAQVQDGFPDNKNVKFETAEMKPGTVLFLPRGTWHYT
                     EAGEPSLSLSITISPPTLLDCAMEQLRWLLLQDSAWREPLYGATATGTMNENLRAHAQ
                     RLLERLPQLVSSLSPEDLIQAPIPVAKRLDQVSAKSRFQRIPDAVLEVEPDKSPKGFT
                     MVNIRLGHSESSTRISACLEIPAEVLAVFRWVEGQHKAFAGSDVQTAFPNLSFEELKK
                     ILATLTRAQFIKMFWFAELAPPARVGTVSRKL"
     gene            complement(2804..>4555)
                     /gene="glmS"
                     /locus_tag="AC16FV_12435"
     CDS             complement(2804..>4555)
                     /gene="glmS"
                     /locus_tag="AC16FV_12435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR2850892.1"
                     /translation="YDSAGIAVLGNGGAERLDRVRAVGKVKALEDTIQQLKTEGQTGI
                     AHTRWATHGVPSEDNAHPHVSHGAIAVVHNGIIENHEKLRKKLQELGYEFSSQTDTEV
                     VAHLVHHHLKKQKDLCKAAMAAIGELEGAYALGIISRDEPHRLIAARKGSPLVVGIGI
                     EEHFIGSDVLALAPVTRNVIYLEDGDIADLRRTEHTIYDASGKKVERPVRVSEASADS
                     VERGQYRNFTEKEIFEQPKAVADTLEGRLGKHRILEEAFGVTAGQIFDNTKAVQIIAC
                     GTSFHAGSVARYWIESLAGLPCNVEIASEYRYRKHVVLPDTLVVTISQSGETADTLAA
                     LQQAKKLGYGHSLAICNVPESSLVRESELTLLTRAGPEIGVASTKAFVTQLTALLLLT
                     IALARRHGLTAEKEKQLVEELEQLPGRIQKVLELSEEIRALSERFVDKQHALFLGRGV
                     HYPIAMEGALKLKEISYIHAESYAAGELKHGPLALVDANMPVIAVAPNDELLEKLKSN
                     LQEVRARGGELYVFSVKGTGVRASNTTKIRMPEVGETTSPILYTVPLQLLAYYVALAK
                     GTDVDQPRNLAKSVTVE"
BASE COUNT          825 a         1370 c         1423 g          937 t
ORIGIN      
        1 ccgcagccag gcgtactgga tgcgattggt gtcctggaat tcgtccacca gcacatggcg
       61 gaagcgcgcc tggtaatgcg cgcgcatgga ttcattgtcg cgcagcaact cgtagctgcg
      121 cagcaacagc tcggcgaaat cgaccagccc ggaacgttgg caggtctcct cgtaggcggc
      181 gtacacccgg cgcagctcgc gctgggtcgg gtcgttggac tcgccgatgt ccttgggtcg
      241 ccgaccctct tccttgtgac cattgatgaa ccacatggcc tgcttgggcg gccagtaggc
      301 ctcgtccatg ttcatgcctt tcatgagtcg cgtgatgact ctgagttggt cttcactatc
      361 aagaatcgtg aatgtttgag gcaggtttgc ttccttccag tgcgaacgca acagccggtg
      421 cgacagcccg tgaaaggtgc cgatccacat gccgcccaca ggcgtgtgca gcatggactc
      481 gatgcgcccg cgcatctcat gggcggcttt gttggtgaag gtcaccgcca gaatggaatg
      541 cggagatacg ccctccaccc ccacgagcca ggcgacgcga tgggtgagca cgcgggtctt
      601 gtcgctgccg gcgccggcca gcaccagcaa tggccccggg ggagcgccca cggcctcgcg
      661 ttgcacgtcg ttgagatcgt ccatcaggtg ggaaacatcc atggcaggat tttaccagag
      721 acacagtgga acccgcggga aatcagcggc gttgccgggt gccgccgggc ctgattagga
      781 atggtcagaa tttggcttta cactgataac cattaataaa acgagcctgt cagctttcga
      841 ggcaaaagct catgtcacta aaaacaaagc tgggagagat gttggcgcct gtcttcagca
      901 agcgcgaaga cgtgctgctg tgcaaggaac tgcaggaggc gcccggcgag gcgccgtctg
      961 gacgcctcga agtagttccg gtgcgccccg aacacctgaa ggcgctggtg caattcatcc
     1021 atgagcacca tggcgatgtg gcgcgttcgc tgcgtatgct cgatgactgc tttcgcaacc
     1081 gttacgaggg tcgcgtggcg tttctgaacg gccaggtcat cggctatcgc tggtgggtca
     1141 cccacaccat gagccatccc cagctaaagc tctatcacct gacgctgcgc gagaacgagg
     1201 tattcgcctt cgggctttac atcgcgcgcg ccttccgcgc ccaaggttat gccggggaat
     1261 tcctggcgat cacccagaaa cagttgctgg acctcggtta caaacgtctc tacaacgccg
     1321 tgggcgccag caatgtcccg gcacggcgcc tgtatgaatc cttcggctcg aaggaactgg
     1381 ggctacatgt ttcgttgagt ttcttttcgc tcgtggcttg ttgtggcggc cgctggctgc
     1441 gttatgaccc ggtgtggatg tgagctgacg agggcgtccc gcgccttcat aacttgcggc
     1501 tcaccgtgcc cacgcgcgcc ggtggcgcca gctcggcaaa ccagaacatt tttatgaatt
     1561 gagcccgagt cagggtggcg aggatttttt tcagttcttc gaaagacaag ttcggaaacg
     1621 ccgtttgcac atctgaaccg gcaaaggctt tgtgctgccc ctcgacccag cggaacaccg
     1681 ccagcacctc ggcagggatt tccagacacg cggagattcg ggtgctcgat tcggaatggc
     1741 ccaatcgaat gttgaccatg gtaaaacctt tcggtgattt gtcgggctcc acttcaagca
     1801 cagcatcagg gattctctgg aagcgggatt ttgcgctgac ttggtcaaga cgttttgcga
     1861 ccgggattgg cgcctgaatc aaatcctccg gcgacaaact ggacacaagc tggggcaagc
     1921 gttcaagcag gcgctgggcg tgggcgcgca aattttcatt catcgtcccg gtggccgtag
     1981 cgccgtacaa gggttcgcgc catgcgctgt cctgcaacaa taaccagcgc aattgctcca
     2041 tggcgcagtc gagcagcgtc ggcgggctga tggtgatgct aagcgagagc gagggctcgc
     2101 cggcctcggt gtagtgccaa gtgccgcgcg gcaggaacag caccgtgccg ggtttcattt
     2161 cggcggtttc gaacttgacg tttttgttgt cgggaaatcc atcttgcact tgcgcataca
     2221 tttcatcgta gggcttgcca cccgggatgt actgcgtgcc ataaggcatg gcaacttcct
     2281 gcaccggagc gtaatgaaag cgcttggtgc cgagcaactg aatggaaatc acatcctgcg
     2341 aatcgtaatg acagccgaga ccgtcctggt acggcgaggc gaaggcgctc atgaccagcg
     2401 agccctcgtt cagtccgagc tcggattcga gtgcgcgcag gaattctggc gcgcccggca
     2461 catacggccc gatgtcctcg aaattcagcg tcagccccat ctcgaacagg atcgccggat
     2521 cgactcggtc gatctggatc atctgatcgc tcttgcgccc gtgggtgaaa cgcagactgc
     2581 cacggtaacg cttcgccagg ttctgcacgc tcgccaactc ttcgcaccgc aacaccgccg
     2641 gccagcgcgc cgggttggcc tgcgccacga acggccgctt gggccagtgc tgggtcagga
     2701 attcatcagc ggctttaggc gacaacagcg ccgcgagaac gggattgttt gctccgtaca
     2761 tctcctcgcc tccaaggggg cgaggataca gagaaaaact ggcttactct acggtcaccg
     2821 atttcgccag attgcgcggc tggtccacgt ccgtcccttt cgccagcgcg acgtaatagg
     2881 ccagcaattg caacggcacg gtgtagagaa tcggcgaggt ggtttcgccc acctcgggca
     2941 tgcggatctt ggtggtgttg ctggcgcgca cgccggtgcc cttgaccgag aacacgtaga
     3001 gctcgccgcc acgggcgcgg acttcctgca agttggattt cagcttttcc agcaactcgt
     3061 cgttgggcgc aaccgcgatc accggcatat tggcgtccac cagcgccagc gggccgtgct
     3121 tcagttcgcc ggccgcatag gactcggcat ggatgtagga gatttccttg agcttgagcg
     3181 ccccctccat ggcgatgggg tagtgcacgc cgcggccaag gaacagcgcg tgctgcttat
     3241 ccacgaagcg ctcggacagg gcgcggattt cctcgctcaa ttccagcact ttctggatgc
     3301 ggcccggcaa ctgctccagt tcctccacca gctgcttttc cttctcggcc gtcagcccat
     3361 gccggcgcgc cagcgcgatg gtgagcagca gcagggcggt gagctgggtg acgaaggcct
     3421 tggtcgaggc gacaccgatc tctgggccgg cgcgggtaag aagggtgagc tccgattcgc
     3481 gcaccagcga cgactccggc acgttgcaga tggcgagcga atggccgtag ccgagcttct
     3541 tggcctgctg taaagccgcg agcgtgtcgg cggtttctcc cgactgtgaa atggtcacca
     3601 ccaacgtgtc cggcaggacc acgtgtttgc ggtagcggta ttcgctggcg atctcgacgt
     3661 tgcacggcag gccagcgagg ctttcgatcc agtaacgcgc caccgatccg gcgtggaagc
     3721 tggtgccgca ggcgatgatc tgcacggcct tggtgttgtc gaaaatctgc ccagcggtga
     3781 cgccaaaggc ctcttccaga atgcgatgtt tgccgagacg cccttcgagc gtgtcggcga
     3841 cggctttcgg ctgctcgaaa atttccttct cggtgaagtt gcggtactgg ccacgctcca
     3901 cggaatcggc cgaggcctcg gacacgcgca ccgggcgctc gaccttcttg ccgctggcgt
     3961 cgtaaatggt gtgctcggtg cggcgcagat cggcgatgtc gccgtcttcg agatagatca
     4021 cgttgcgcgt caccggcgcc agcgccagca cgtccgagcc gatgaaatgc tcctcgatgc
     4081 cgatgccgac caccagcggg ctgcccttgc gcgcggcgat caggcgatgc ggttcatcgc
     4141 gactgatgat gccgagggca tacgcgcctt cgagctcgcc aatcgcggcc atggccgcct
     4201 tgcacaaatc cttctgcttt ttcagatggt ggtgcaccag atgcgccacc acctcggtat
     4261 cggtctggga gctgaattcg taaccgagtt cctgcagctt tttccgcagt ttttcgtggt
     4321 tttcgatgat gccgttgtgc accacggcga tggcgccgtg gctcacgtgc gggtgggcgt
     4381 tgtcctcgct cggcacgccg tgcgtggccc agcgggtatg ggcgatgccg gtctggccct
     4441 cggttttgag ctgctggatg gtgtcctcga gtgccttgac cttgccgacc gcgcgcacgc
     4501 ggtcgagacg ttccgcgccg ccgttgccga gcacggcgat cccggccgag tcata
//
LOCUS       DBNGZE010000483         8717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_831695, whole genome shotgun sequence.
ACCESSION   DBNGZE010000483 DBNGZE010000000
VERSION     DBNGZE010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_831695"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16FV_12440"
     CDS             complement(<1..451)
                     /locus_tag="AC16FV_12440"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR2850893.1"
                     /translation="MTDTNRRPPLYTSHRRALTVLYADLESYALNQREVFVGTAGSVI
                     ARTNASGFRFYAHQFYDGEGKKRERYLAGPMGDPKADAAAAEQRERIKELKEIVPSLR
                     LLGREGFNLVDAKTYATLASLHNHGVFAAGGMLIGSHAYGVLLNRLGV"
     gene            783..1073
                     /locus_tag="AC16FV_12445"
     CDS             783..1073
                     /locus_tag="AC16FV_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR2850894.1"
                     /translation="MKIRPLHDRVNVRRLEEERKSPGGIVIPDTAKEKPIQGEVIAVG
                     HGKILEDGKVRPLDVKVGDKILFGKYSGTEVKVGNEELLVMREEDIMGVVEK"
     gene            1106..2758
                     /gene="groL"
                     /locus_tag="AC16FV_12450"
     CDS             1106..2758
                     /gene="groL"
                     /locus_tag="AC16FV_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648310.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR2850895.1"
                     /translation="MAAKDVLFGDPARTRMLRGVNILADAVKVTLGPKGRNVVLEKSF
                     GAPTVTKDGVSVAKEIELKDKYENMGAQMVKEVASKTSDVAGDGTTTATVLAQAMLRE
                     GLKSVAAGMNPMDLKRGIDKTVVAAVEALKKISKPCSDPKEIAQVGTISANADESIGK
                     IIADAMEKVGKEGVITVEEGSGLENQLDTVEGMQFDRGYLSPYFINKQETMSAELENP
                     SILIYDKKISNIREMLPILEGVAKQGRPLLIISEDVEGEALATLVVNNIRGILKVCAV
                     KAPGFGDRRKAMLQDVAILTAGTVISEELGMSLEKADASMLGSAKRVTVDKENTTIID
                     GAGKKQDIEARIKQIKAQIEEATSDYDKEKLQERMAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALVRAVTAIKGIKGDNHDQDVGIQIAMRAMQEP
                     LRQIVENAGIEGSVVYHKVADGKEVNFGYNAATGEYGDMFKMGILDPTKVERIALQNA
                     ASVAGLMITTEAMVAEMPKEESPAGGGMGGMGGMGGMGGMDM"
     gene            complement(2823..3512)
                     /locus_tag="AC16FV_12455"
     CDS             complement(2823..3512)
                     /locus_tag="AC16FV_12455"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR2850896.1"
                     /translation="MTPSPAALAAEPERPPVKAIIPEKIEAYCLRHTTVGDKLLRELE
                     KYTQRHCADPQMLIGPFEGALLVLLVRLCGARRVLEIGTFTGYSALCMAEALPKNGQL
                     ITCEIRPKHAEIAQSFFDCSPHGRKIQIRLGPALETLASLPRSTSFDFVFIDADKENY
                     VNYYEAVLPRLRPGGLIAADNVLWSGRVLAPKKKTDRAVVAFNKHVRRDKRVECVMLP
                     VRDGVSLIRKR"
     gene            complement(3481..4542)
                     /locus_tag="AC16FV_12460"
     CDS             complement(3481..4542)
                     /locus_tag="AC16FV_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR2850897.1"
                     /translation="MKSETQIFENIRDYYGRVLKTNRDLKTGACCAGDSFAPSIREIL
                     KQIHPNVVAKFYGCGSPIPPELTGRTVLDLGSGSGRDCFILSKLVGPGGRVIGVDMTP
                     EQLDTARRHVGYHMEKFGFAKPNVEFLSGYIEDLESLGIASDSVDVVVSNCVINLSPD
                     KERVFAEIFRVLKPGGELYFSDVFADRRIPEHLAEDPVLLGECLGGALYIEDFRRLLA
                     RVGCRDYRIVNNSRLALTDPAIERKAGMIGFHSITVRAFKLDLEDRCEDYGQVAYYLG
                     TVPEHPHRFMLDDHHVFETGRPHPVCGNTAAMLSQTRYAPHFKIVGNTRVHYGLFDCG
                     PKSATTGSENDTKPSGSCC"
     gene            complement(4545..5663)
                     /locus_tag="AC16FV_12465"
     CDS             complement(4545..5663)
                     /locus_tag="AC16FV_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR2850898.1"
                     /translation="MELLTAVFFGVFLAYANGANDNFKGVATLFGSGTTDYRVALRWA
                     TAMTLVGALVALALASELLHTFSGRGLVPESVLQTRQFPMAVAFAAAFTVWLATRLGF
                     PVSTTHAIVGAFVGAGLAASPAQINFLGLTSNFFLPLVLGPLLALGGVMLLCPVFQWI
                     RTRLGVRKEICVCVGTEVMARFPVGTTPPQAIAAMAMPSVRIAEESRCREQYVGTFVG
                     VNAKTVLDAAHFASAGAVCFARAVNDTPKIAAILLASQALSSNLVIATVALAMVFGGL
                     VSSRKVAETMAHRVTAMNAGQGFTANLVTSALVLLASKFGLAVSTTHVSCGALFGTGV
                     VNGQAHWKTIAGIIAAWIVTLPVAAASAGLGFLILGGG"
     gene            complement(5809..6636)
                     /locus_tag="AC16FV_12470"
     CDS             complement(5809..6636)
                     /locus_tag="AC16FV_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR2850899.1"
                     /translation="MRGLCSLLLILLPALALAGEKPPAGKFTNAECVSCHASRDAKLV
                     AAWRASGHARTAPRADCVTCHGNAHLGSNQRARRDSACVGCHERLNRAVVHIYATSKH
                     ATILKLEENRWDWSQSLARGNYRAPGCAYCHLHAGEHDTRLSVATWQPFQPEESSVRE
                     PIAARWQAICGECHAPRYVAEQTHTGARMQEIARMKLREAREVMTRYQDVLSAEDRRE
                     MDTLFTVMQTHGHNVYLGVGHQSPDYQWWHGQPALDGDLLRIKGVIAERERRRAGQP"
     gene            complement(6636..7991)
                     /locus_tag="AC16FV_12475"
     CDS             complement(6636..7991)
                     /locus_tag="AC16FV_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR2850900.1"
                     /translation="MRRYIWVTLALTLLAVPAGYAYVYWAKSGPGAGLYSREWKPEPL
                     HAYWDPDKFYQSPQTLQGEFKGEECIACHSAVTPGIVNDWRASRHAHPGQGKQTVYCS
                     ACHGSNHQALHMPTPKVCAQCHENRVAQFQDEKRYGFPSHALAMERAMDAKHFADKPK
                     AEVAACLQCHSVANKCDSCHTRHRFSPAEARRPEACVTCHSGPPHPDDDSYFASAHGK
                     KYLADVAKQDWSKPLAKGNYATPTCAYCHMHEGQHQVADKTIWKFGLLEVNPNTSGNE
                     VRRAAWVKVCSDCHEAGWSAQQLKGLDRERKQAWGKLYKAEYLLKDLRSDNLLHPGPR
                     ERPPYPMDALETLFPKAHIGYYDGQASAFYNVSPIERDYFEMWYFASLGAYKSAAHGD
                     GLSVGRWHAVMDKSLSVIETNAQALRVQGGNEKKSLGRRADPGRLWLEGEYTEHNREH
                     N"
     gene            complement(7995..8246)
                     /locus_tag="AC16FV_12480"
     CDS             complement(7995..8246)
                     /locus_tag="AC16FV_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850901.1"
                     /translation="MRRALFIILLFILALPVHAHHTKEHMLGAPSPPPAVVTPVAPDD
                     AGSSRLFWALGPFFFLVAVGVLRWGYRHRRDGKDKNMRG"
     gene            complement(8249..>8717)
                     /locus_tag="AC16FV_12485"
     CDS             complement(8249..>8717)
                     /locus_tag="AC16FV_12485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR2850902.1"
                     /translation="AEEKLRASPREAKAAREEGVEFRFRHTPVEIIGGEAAQSVRFQT
                     PDGEERSEACDLVILAFGFVAQPPEWLASFGIATDAQRHIRVDAHGRTTHPKIYAGGD
                     MTRGPDLVVTAAADGRRAAEGMLESFRPLARTRMVLRNLTAKATPRPVTAMEG"
BASE COUNT         1740 a         2645 c         2731 g         1601 t
ORIGIN      
        1 ggacgcccag gcgattcagc agcaccccgt aggcgtgcga gccaatgagc atcccgccgg
       61 ccgcaaatac cccatggtta tgcagactgg cgagggtggc ataggtcttg gcatcgacca
      121 gattaaaccc ctctcgtcct agcaaacgca gcgagggcac gatctccttc agttccttga
      181 tccgttcgcg ttgctcggcc gcggcggcat cggccttcgg gtctcccatg ggtccggcca
      241 agtagcgctc gcgcttcttg ccctcgccgt catagaactg gtgggcgtag aaacgaaaac
      301 ccgaggcatt cgtgcgcgcg atcaccgagc cggcggtccc cacgaagacc tcgcgctgat
      361 tgagagcgta gctttccaag tcggcgtaca acaccgtcag cgcgcggcgg tgcgacgtat
      421 acaggggcgg ccgtcggttt gtatccgtca tactaaaatg ctagtggata ctatagtatc
      481 cgtcaacagt caataatgac ggatacatgc gggtggtgtg gctccttctg gccggtaaat
      541 cctccatttt ttggcgccgt gggcgattgg accggactat cagctttaaa tggcgcctga
      601 caaccgcccc ttcccccgtt gacaacctga catcccccat tatcattagc actcacttga
      661 accgagtgct aacaatcctc tggcacccgg ttatcagtgc tttaacccaa catacggaaa
      721 aagtgcgaaa tttttaagga ggaaatatga gtgcacaacc ccttaccaaa ggagaacccg
      781 ctgtgaaaat ccgtccgctt cacgaccgcg tgaacgtgcg ccgcttggaa gaagagcgta
      841 aatcacccgg tggcatcgtc atccccgaca ccgccaagga aaagccgatc cagggcgaag
      901 tcatcgccgt gggtcacggc aagattctcg aagacggcaa ggtccgtccg ctggacgtca
      961 aggtcggaga caagattctc ttcggcaagt actccggcac cgaggtcaag gtcggcaacg
     1021 aagagctgct cgtgatgcgt gaagaagaca tcatgggcgt ggtcgagaag taatcaaatc
     1081 agactgaatt tcaggagagt aaatcatggc tgctaaagac gtactgtttg gcgatcccgc
     1141 ccgcacgcgc atgctgcgcg gcgtcaacat cctggccgac gcggtcaagg tcacgctcgg
     1201 tcccaagggc cgcaacgtgg tgctggaaaa atccttcggc gccccgacgg tgaccaagga
     1261 cggcgtcagc gtcgccaagg aaatcgaact caaagacaaa tacgaaaaca tgggcgcgca
     1321 gatggtgaag gaggtcgcga gcaagacctc cgacgtcgcc ggcgacggca ccaccacggc
     1381 gaccgtgctg gcgcaggcaa tgctgcgcga gggactgaag tccgtcgccg ccggcatgaa
     1441 cccgatggac ctgaagcgcg gcatcgacaa aaccgtcgtg gcggcggtcg aggcgctgaa
     1501 gaaaatctcc aagccctgtt ccgacccgaa ggaaatcgcc caagtcggca ccatctccgc
     1561 caacgccgat gaatccatcg gcaagatcat cgccgatgcc atggagaagg tcggcaagga
     1621 aggcgtgatc acggtcgagg aaggctcggg tctcgagaac cagctggata ccgtggaagg
     1681 catgcagttc gaccgcggct atctgtcgcc gtacttcatc aacaagcagg agacgatgag
     1741 cgccgagctc gaaaatccgt cgatcctgat ctacgacaag aagatctcca acatccgcga
     1801 gatgctgccg atcctggaag gcgtggccaa gcagggccgt ccgctgctga tcatttccga
     1861 ggacgtcgag ggcgaggcgc tcgcgacctt ggtggtgaac aacatccgcg gcatcctcaa
     1921 ggtctgcgcc gtgaaggccc cgggcttcgg cgaccgccgc aaagccatgc tgcaggatgt
     1981 cgcgatcctg accgcaggca cggtgatctc cgaggagctc ggcatgagtc tcgagaaggc
     2041 cgatgccagc atgctcggct ccgccaagcg tgtcacggtg gacaaggaaa acaccaccat
     2101 tatcgatggc gccggcaaga agcaggacat cgaggcgcgc atcaagcaga tcaaggccca
     2161 gatcgaggaa gcgaccagcg actacgacaa ggaaaagctg caggagcgca tggccaagct
     2221 ggccggcggc gtggccgtca tcaaggttgg cgccgcgacc gaggtcgaga tgaaggaaaa
     2281 gaaggcacgc gtggaagacg cgctgcacgc gacccgcgcg gccgtggaag aaggcatcgt
     2341 ccccggcggc ggcgtggcgc tggtgcgcgc cgtcacggcg atcaagggta tcaagggtga
     2401 taaccacgac caggacgtcg gcatccagat cgccatgcgt gcgatgcagg agccgctgcg
     2461 ccaaatcgtg gagaacgccg gcatcgaagg ctcggtggtg taccacaagg tcgccgatgg
     2521 caaggaagtc aacttcggct acaacgccgc gaccggcgag tacggggaca tgttcaagat
     2581 gggcatcctc gacccgacca aggtggagcg catcgcgctg caaaacgcgg cctccgtcgc
     2641 cggcctcatg atcacgaccg aagccatggt cgccgaaatg ccgaaagagg aatctccggc
     2701 tggcggtggc atgggcggga tgggtggaat gggtggcatg ggcgggatgg atatgtaagg
     2761 ttgtcctctc ttgcttgagt agaactgaaa gccccgcgat gcggggcttt cttttttggt
     2821 tctcaacgtt ttcggatcaa actcacgcca tcgcgcacgg ggagcatcac gcattcgaca
     2881 cgcttgtcgc ggcggacgtg tttgttgaag gcgacgacgg cgcggtcggt ttttttcttc
     2941 ggcgcgagca cacggcccga ccacagcacg ttgtcggccg cgatcagtcc gccgggccgc
     3001 aggcgcggca gcaccgcttc gtagtagttc acgtaattct ctttatccgc atcaatgaaa
     3061 acgaaatcga agctcgtcga gcgtggcagg ctggcgagcg tttcgagcgc cgggccgagg
     3121 cgtatctgaa tcttgcgtcc gtgcgggctg cagtcaaaaa acgactgcgc gatctcggcg
     3181 tgtttcggcc tgatctcgca ggtgatgagc tggccgttct tgggcaatgc ttcggccatg
     3241 cacagcgcgg agtagccggt gaaggtgccg atctcaagca cgcgccgcgc accgcacagg
     3301 cgcaccagca ggaccagtag cgcgccctcg aacggaccga tcagcatctg aggatcggca
     3361 cagtgtcgct gggtatattt ttccagttcg cgcaacagtt tgtcgcccac cgtggtatga
     3421 cgcaggcaat atgcttcgat tttttcaggg attatcgctt tcacgggtgg cctttccggc
     3481 tcagcagcaa gagccgctgg gcttggtgtc attctctgaa ccggtggtag cggacttcgg
     3541 tccgcaatcg aacaggccgt aatggacgcg ggtgttaccg acgatcttga aatgcggcgc
     3601 gtagcgcgtt tggctcagca tcgcggcggt attgccgcag accggatgcg gccggccggt
     3661 ctcgaacaca tgatgatcgt ccagcatgaa acgatgcggg tgctctggaa cagtgccgag
     3721 gtaatacgcc acctgcccgt aatcttcgca acgatcttcg aggtcgagct tgaaggcgcg
     3781 caccgttatg gaatgaaaac cgatcatgcc ggccttgcgt tcgatggcgg gatcggtgag
     3841 cgcgagtctt gaattgttca cgatccggta atcgcggcac ccgacccggg ccagcagccg
     3901 ccggaaatcc tcgatataaa gcgcgccgcc cagacactcg cccagcaata ccggatcctc
     3961 ggccaggtgc tccgggatgc gacggtcggc gaatacgtcg gaaaaataca gctccccgcc
     4021 cggtttgagc acgcggaaga tttccgcgaa tacgcgctcc ttgtccggcg agagattgat
     4081 aacgcaattg gagaccacga cgtcgaccga gtcgctcgcg ataccgaggc tttccaggtc
     4141 ctcgatgtat cccgaaagga actcgacatt gggttttgcg aaaccgaatt tctccatgtg
     4201 atagccgacg tgacggcgtg cggtgtcgag ctgttccggt gtcatgtcca cgccgatcac
     4261 gcgcccaccg ggaccgacga gcttcgagag aatgaaacag tccctgcccg aacccgaccc
     4321 cagatcgagc accgtccggc ccgtcaattc gggcggaatc ggcgagccgc agccgtagaa
     4381 tttggcgacg acgttggggt gaatctgttt gagaatttcc cggattgagg gagcgaacga
     4441 atcgccggcg caacaggcgc cggttttgag gtcgcggttg gtcttgagca cgcgcccgta
     4501 atagtctctg atgttctcaa atatctgtgt ctcgcttttc atctttaacc cccgcctaaa
     4561 atgagaaaac ccaacccggc cgacgcggcg gcgacgggca aagtgacgat ccacgcagcg
     4621 ataataccag cgatggtttt ccagtgtgcc tgtccattga ccacgccggt tccgaagagt
     4681 gccccgcagg atacgtgggt tgtggaaacc gcaagcccga acttcgacgc cagcagcacc
     4741 agggcgctgg tcacgagatt cgccgtgaat ccctggccgg cattcatcgc cgtcacgcga
     4801 tgcgccatgg tttcggccac cttgcgggag gagaccaggc caccgaacac catcgcgagc
     4861 gccacggtgg cgatgacgag gttcgatgac agcgcctgac tggccagcag tatcgccgca
     4921 atcttgggcg tgtcgttcac tgcgcgggcg aaacagaccg cgccggcgct ggcgaagtga
     4981 gcggcgtcca gaacggtttt ggcattcacg cccacgaagg tgccgacata ttgttcgcgg
     5041 caacggcttt cctctgcgat cctgacgctg ggcatggcca tcgccgcgat cgcttgcggc
     5101 ggtgtggtgc ccacggggaa ccgcgccatc acctcggttc ccacgcatac gcagatttcc
     5161 ttccgtaccc caagccgggt acgtatccat tggaagaccg ggcacaacaa catcacgccg
     5221 ccgagcgcga gcagagggcc gagcaccaat ggcagaaaga agttcgatgt cagtccgaga
     5281 aaatttatct gcgcaggcga agccgccaga cctgcgccca cgaacgctcc cacaatcgcg
     5341 tgggtggtgg agacaggaaa cccgagccgg gtcgccagcc acaccgtgaa cgcagcggcg
     5401 aaagcaaccg ccatggggaa ttgtctggtc tgcaacaccg attccgggac caggcctctg
     5461 ccggagaagg tatgcagcaa ctccgaggca agcgccaggg ccacgagcgc accgaccaac
     5521 gtcatcgccg tcgcccagcg cagcgccacg cgataatccg tggttccgct tccgaagagc
     5581 gtggcgaccc ctttgaagtt gtcgttggcg ccgttggcgt aggcgagaaa caccccgaag
     5641 aaaacggcgg tgagcagctc cattacgtga tttccctcac ttgatctccg cgcggaacgg
     5701 aatatatccg ctggtccggc ttgcgcaaga gtaccgcaag gaagccgccc caaagcaaca
     5761 cgccggggag catcgcttcg gccacgcgcg ctaccgtttc gcgcgggttc atggctggcc
     5821 ggcgcgccgg cgctcccgtt ccgcgatcac ccccttgatg cgcaagaggt cgccgtcgag
     5881 tgccggttga ccgtgccacc attggtaatc cggtgactga tgtccgacgc caagatacac
     5941 gttatgtcca tgcgtttgca tgacggtgaa caaggtatcc atctcgcgcc ggtcttcagc
     6001 agacagcacg tcttggtaac gtgtcatgac ctcgcgcgcc tcgcgtaatt tcatgcgtgc
     6061 aatttcctgc atgcgtgcgc cggtgtgtgt ttgctcggcg acgtagcgcg gcgcgtggca
     6121 ttcgccgcat atggcctgcc accgggctgc gatgggttcc cggacggatg attcttccgg
     6181 ctggaatggc tgccacgtcg ctaccgaaag gcgcgtgtca tgttcgccag catgcagatg
     6241 gcagtaggcg cagccgggcg cgcggtaatt tccgcgcgcg agcgattgcg accagtccca
     6301 ccggttttct tcgagtttca agatcgtggc gtgcttggag gtcgcgtaaa tgtgcacgac
     6361 cgcgcgattc aatctttcat gacaacccac acaggcgcta tcgcgccgcg cgcgttggtt
     6421 gcttcccaaa tgtgcgttgc cgtggcaggt aacgcagtcg gccctaggcg cggtgcgcgc
     6481 atgcccgctc gcgcgccagg cggcgacgag tttcgcatcg cggctggcgt gacagcttac
     6541 gcactccgca ttcgtgaatt ttccggccgg cggcttttcg cccgccaagg cgagcgccgg
     6601 cagcaaaatg agcaaaaggg aacagagccc gcgcatcaat tgtgttcccg gttgtgttcg
     6661 gtgtactcgc cttcgagcca gagccggccc gggtcggcgc gccggccaag cgatttcttt
     6721 tcgttccctc cctgtacgcg caacgcctgg gcgttggttt cgatgacgct gagggacttg
     6781 tccatcaccg cgtgccagcg cccgaccgaa agaccgtcgc catgcgcggc gcttttgtac
     6841 gcgccgagac tggcgaaata ccacatctcg aaataatcgc gctcgatggg cgagacgttg
     6901 tagaaggccg aggcctgacc gtcgtaataa ccgatatgcg ccttcgggaa gagcgtttcc
     6961 aacgcgtcca tgggataggg cggacgctca cgcggtcccg gatgcagcag gttgtcgctg
     7021 cgtaaatcct tgagcaggta ctcggccttg taaagcttgc cccaggcctg cttgcgctcg
     7081 cggtcgagcc ccttcagttg ttgcgccgac cagccggcct cgtggcaatc gctgcatacc
     7141 ttgacccagg cggcgcgacg cacctcgttg cccgaggtgt tcggatttac ttcgagtagc
     7201 ccgaacttcc atatggtctt gtccgcaacc tgatgctgac cctcgtgcat gtggcaatag
     7261 gcgcaggtcg gcgtcgcgta gttgcccttg gcgagcggtt tgctccagtc ctgtttcgcg
     7321 acatccgcca gatatttttt tccatgcgcg gaggcaaaat aactgtcgtc gtccggatgc
     7381 ggcgggccgg aatggcaggt gacgcaggcc tcgggccggc gcgcctcggc ggggctgaag
     7441 cggtggcgcg tatggcagga gtcgcatttg ttggcgaccg aatggcattg caggcaggct
     7501 gcgacctcgg ccttgggttt gtcggcgaag tgcttggcgt ccatagcgcg ttccatcgcg
     7561 agcgcgtggc tcgggaagcc gtagcgcttt tcgtcctgga actgggcgac gcggttctcg
     7621 tgacactgcg cgcagacctt cggtgtcggc atgtgcagcg cctggtggtt cgatccgtga
     7681 caggcggaac agtaaaccgt ctgtttgccc tgtccgggat gggcgtggcg gctcgcgcgc
     7741 caatcattga cgatgcccgg cgtgacggcg ctgtggcagg caatgcactc ctcgcccttg
     7801 aactcgccct gcaaggtctg cggcgactgg taaaacttgt ccgggtccca gtaggcgtgc
     7861 agcggttccg gtttccattc gcgcgaatac agacccgcgc ccggcccgct cttggcccag
     7921 tagacatagg cgtaacccgc gggaaccgcg agcagcgtga gcgcgagcgt gacccagata
     7981 tagcggcgca tggttcagcc ccgcatgttt ttgtctttgc catcccggcg atgacggtat
     8041 ccccagcgca acacgcccac ggcaaccagg aagaaaaacg gaccgagcgc ccaaaacagg
     8101 cggctgctgc cagcgtcatc cggcgccacc ggagtgacaa cggccggtgg tggagacggc
     8161 gcgccgagca tatgttcctt ggtgtgatgg gcgtgcacgg gcagcgcgag gataaaaagc
     8221 aaaatgataa aaagtgctcg ccgcacgttc agccctccat cgccgtcacc ggacgtggcg
     8281 ttgccttggc cgtcaggttg cgcaatacca tgcgcgtgcg cgccaacggg cggaaacttt
     8341 cgagcatgcc ctcggccgcg cgtctcccgt ccgccgccgc ggttaccacc agatcaggcc
     8401 cgcgggtcat gtcgccaccg gcgtagattt tgggatgggt cgtgcggccg tgtgcgtcca
     8461 cgcggatgtg tctctgcgca tcggttgcga tgccgaagga cgcgagccac tcgggcggct
     8521 gcgcgacgaa gccgaaggcg agaatcacca gatcgcaggc ttcgctgcgt tcttcgccat
     8581 ccggcgtctg gaagcgcacc gactgcgccg cctctccgcc gatgatttca accggcgtgt
     8641 gacggaagcg gaattccacg ccttcctcgc gcgcggcctt ggcctcgcgc ggcgaggcgc
     8701 gcaacttctc ttcggcg
//
LOCUS       DBNGZE010000484         7607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_415527, whole genome shotgun sequence.
ACCESSION   DBNGZE010000484 DBNGZE010000000
VERSION     DBNGZE010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_415527"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC16FV_12490"
     CDS             <1..332
                     /locus_tag="AC16FV_12490"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR2850903.1"
                     /translation="QRIAIVDVSSKAGIHHHGRLVLINPEVFEKKGETAGREGCLSVP
                     DYTGNVARAERIRLQAQDIDGARLSYDCEGYEARAIQHEIDHLDGFLFLDRLVSRRDL
                     FRRKNYK"
     gene            complement(323..817)
                     /locus_tag="AC16FV_12495"
     CDS             complement(323..817)
                     /locus_tag="AC16FV_12495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR2850904.1"
                     /translation="MYRKHVVILVLIAVIAAIVYLLWPRASSDTARPDRGAPVTSLTT
                     DEGRYLFNVTLHTPEEIAGLLARAEDLAKTRNADSRHTGIALVLHGPEIEIFAKKNYA
                     RFRNTVDQAARLDAGRIIEVKMCRTEMKHRGIREEDIPAFIELVPYGPDEEMRLRRNG
                     YVYL"
     gene            complement(847..1689)
                     /gene="nadC"
                     /locus_tag="AC16FV_12500"
     CDS             complement(847..1689)
                     /gene="nadC"
                     /locus_tag="AC16FV_12500"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR2850905.1"
                     /translation="MTTLPNDIPETVQRALAEDVGQGDLTAALIPADTQVEAQVITRV
                     TAVLCGTAWFDEVFRQVDKRVSIDWTAHDGDSISSGQTLCILRGPARALLTGERTALN
                     FLQLLSGTATLARKYVDAVRGTRAVILDTRKTVPGLRRAQKYAVACGGGQNHRMGLYD
                     AILIKENHIAAAGSIVAALRAARASAPSGVSVEIEVENLEQLRKALESGAVRILLDNF
                     RIEELKSAVREASGRAKLEASGGIDLSNIRAFAETGVDFISIGDMTKNVRALDLSMRF
                     VHSS"
     gene            complement(1695..3137)
                     /gene="nhaD"
                     /locus_tag="AC16FV_12505"
     CDS             complement(1695..3137)
                     /gene="nhaD"
                     /locus_tag="AC16FV_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HIR2850906.1"
                     /translation="MKNHHFSLAMPAVLTALLPVTACAMESGTLAVPDLTTGMLGILA
                     VITFVVAYGLVISEEFSLLRKSKPMMVAAGVIWVLVAIAYAQRGDTETAARALRANFL
                     EYAELMVFLLSAMTYINTMEERSVFRALRSWMVSSGFSLRTIYWLAGLFAFFISPFAD
                     NLTTALLMAAVVLAVAGDNKRFVAVSCISIVVGANAGGAFSPFGDITTLMVWQKGVVR
                     FEEFFDLFIPSLVNWLVPAAIMAFSVPRGKPAAATERIRVKHGGYVVIALFLLTIALT
                     VSLHQFLHLPPFLGMMTGLGLLKVYGYYIRRHEIRRTSSAEPEIEAFQVPEGFKPAVK
                     PFDIFISMKRVEWDTLMFFYGVVLCVGGLGALGYLAALSHSLYQGLGATQANILIGLA
                     SAVIDNIPIMYAVLSMNPDMSHGQWLLVTMTAGVGGSLLSIGSAAGVGLMGQARGIYT
                     FFSHLKWTWAVALGYAASIWVHFLINRSLF"
     gene            3333..3677
                     /locus_tag="AC16FV_12510"
     CDS             3333..3677
                     /locus_tag="AC16FV_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2850907.1"
                     /translation="MKPEYANSLPDGWDRFSRFFFALGDTTRQQILLLFEPGEEICVN
                     DIARLFKISRPAISHHLKILRNAELLVCEKRGKEVYYRVNHDHCAEVMRVVREFIVTQ
                     APPSTLTASAFR"
     gene            3867..5177
                     /locus_tag="AC16FV_12515"
     CDS             3867..5177
                     /locus_tag="AC16FV_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2850908.1"
                     /translation="MQVSSISIPPTASRREIFAWAMYDFANSGYTTVVLTAVFNAYFV
                     GVVAGDGADKSGAATLLWTVAMAITNILVLASAPVLGAIADHGAHKKRFLALTTVGCV
                     LFTALLGFVGRGDVVLGMTLVILASLMFASGENVIAAFLPEIASQKDMGRISGYGWSL
                     GYLGGLLVLGLCLAYIQWAQSRGEIAEQFVPVTMWITAVMFAHAALPTFVWLRERAVP
                     QPLAPGENYIRIGFARVRHTLTHARHYRDLFRFLVTLAVYYCGINTVVVLAAIYAQET
                     MGFTMQENIILILVVNVTAAAGALTFGLVQDRLGSVRTLAITLTVWVVALVLAYFIES
                     RAGFWVVANLVGLALGSSQSAGRALVGQFSPPERAAEFFGLWGLAGKLAAVIGPLVYG
                     LVTYLTHGNHRQALLATTTFFIVGLLLLMTVNERRGRAAAVHPD"
     gene            5194..5958
                     /locus_tag="AC16FV_12520"
     CDS             5194..5958
                     /locus_tag="AC16FV_12520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR2850909.1"
                     /translation="MTVIAFLALAAGVVLLVWLWLRLLRACEAANGADWGGRWLNRLD
                     GLNRIFCRRFHSLVYEPLNLPAAGGALVASNHVSGLDPLLLIAASPRPLRFLIAREEY
                     DRWWLRWLFRRIGCIPVERTRNTRSAFEAARKALKRGEVVALFPHGRIHLDHHEPIPL
                     KRGIALLSSLTGVPIHPVRIEGVRAQGMTVTAVFVPSRACLLSFAPLHCAGLEDEHCL
                     EELWDRLALSGEERRHDNPHRQRRSGKSPDDGETAQ"
     gene            6032..>7607
                     /locus_tag="AC16FV_12525"
     CDS             6032..>7607
                     /locus_tag="AC16FV_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR2850910.1"
                     /translation="MRMDKLTTKFQQALADAQSLALGRDHQFIEPAHLMTALLDQEGG
                     TTRHLLTKSDVNVNALRTKLSQILDRMPTVEGAGGEVHVSNNLANLLNLTDKYAQKRG
                     DQYISSELFLLAALEDKGEFGRLLKEAGVSKNGIEKAIETMRGGQKVEDQNAEEGRQA
                     LEKYTIDLTERAEQGKLDPVIGRDDEIRRCIQILQRRTKNNPVLIGEPGVGKTAIAEG
                     LAQRIINSEVPEGLKGKRLLALDMGALIAGAKFRGEFEERLKAVLKDLSKQEGRIILF
                     IDELHTMVGAGKAEGAMDVGNMLKPALARGELHCIGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPTVEDTIAILRGLKEKYEVHHGVDITDPAIVAAAMLSHRYITDRQLPDKAID
                     LVDEAASRIRMEIDSKPESMDRLDRRLIQLKIEREALKKETDEASKQRLATLEGEIKK
                     LEKEYSDLEEVWKSEKAAVHGAQHIKEALDRARLDFETARRAGDLARMSELQYGRIPE
                     LEKQLNAAQAAEGKERK"
BASE COUNT         1504 a         2308 c         2363 g         1432 t
ORIGIN      
        1 tccagcgtat tgctattgtg gatgtctctt ccaaggccgg tattcatcat catgggcgcc
       61 tggtacttat aaatcccgag gtgtttgaaa aaaaaggcga gaccgccggg cgcgaaggtt
      121 gcctgtccgt accggactac accggcaatg tcgcgcgcgc cgagcgcatt cgtttacagg
      181 cgcaggatat cgacggcgcg agactgagct acgactgcga agggtatgag gcacgcgcga
      241 tacagcacga aatcgaccac ctcgacggat ttctctttct ggatcggctg gtcagccggc
      301 gcgatctgtt ccggcgcaag aattacaaat aaacgtaacc gttgcggcgc aggcgcatct
      361 cttcatccgg tccgtacggc accagctcga tgaaggccgg gatgtcttct tcccgaatgc
      421 cgcggtgttt catttcggtg cggcacattt tcacctcgat aatgcgcccg gcatcgagtc
      481 gcgcggcttg gtccacggtg ttccggaacc gcgcatagtt tttcttcgcg aatatttcga
      541 tctcgggccc atgcaatacc agcgcgatgc cggtgtggcg gctgtccgca ttcctggttt
      601 ttgccagatc ctcggcgcgc gccaacaggc cggcgatttc ttccggcgtg tgcaacgtga
      661 cgttgaacag gtagcgtcct tcatccgtgg tgaggctcgt gaccggcgcg ccacgatccg
      721 gacgcgcggt gtcggaggaa gcgcgcggcc agagcagata aacaatggcc gcgatgacgg
      781 cgatcaggac caggatgaca acatgtttgc ggtacatgcc ggactcccta tttgtctgac
      841 gcggaatcag gatgaatgaa caaagcgcat ggacaagtcc agcgcccgga cgttcttggt
      901 catgtccccg atcgaaatga agtccacgcc ggtttcggca aaagcacgga tgttggagag
      961 atcgatcccg cccgaggcct cgagtttggc gcgcccactt gcctcacgaa cggcggattt
     1021 caattcttca atcctgaaat tgtcgagcag aatacgcacg gcgccggatt caagcgcctt
     1081 gcgcagttgt tcgagattct ccacctcgat ttccacggac acgcccgaag gcgcggaggc
     1141 cctagccgcg cgcagcgcgg cgacgatcga gcccgccgca gcgatgtgat tttccttgat
     1201 caggatggcg tcgtacaggc ccatgcggtg attttgtccg ccgccgcagg ccacggcgta
     1261 tttctgggcg cggcgcagtc ccggtacggt cttgcgcgtg tccaggatga ccgcgcgcgt
     1321 tccgcgcacg gcgtccacgt atttccgcgc cagcgtcgcg gtcccggaga gcagttgcaa
     1381 gaagttgagt gccgtgcgct ccccggtgag caacgcgcgc gccggtccgc gcaggatgca
     1441 tagtgtttgt ccagaagata tggaatcacc gtcgtgagca gtccagtcga tgctgacacg
     1501 tttatccact tgccggaaaa cctcatcgaa ccaggccgtg ccgcacagca ccgctgttac
     1561 gcgcgtgata acctgcgcct caacctgcgt gtcggcggga atgagcgcgg cggtgagatc
     1621 gccctggccg acgtcctccg ccagagcacg ctgcactgtt tccggaatat cgttgggcag
     1681 ggttgtcatc aagatcagaa caggctgcga ttgatcagga agtgcaccca gatgctcgcg
     1741 gcgtaaccga gcgcgacggc ccaggtccat ttgaggtgcg agaagaaggt gtatatgccg
     1801 cgcgcctgac ccatgaggcc gacacccgcg gccgaaccga tggacagcag cgaaccgccg
     1861 acgccggcag tcatcgtcac cagcaaccac tgaccgtgcg acatgtcggg gttcatgctg
     1921 agcacggcgt acatgatggg gatgttgtcg atgacggcag aggcgagacc gatcagaatg
     1981 tttgcctgcg tcgcgcccag accctgatac agggaatgcg atagcgccgc gagatagccc
     2041 aatgcaccga gaccgccgac gcacagcacc acgccgtaga agaacatcag cgtgtcccat
     2101 tccacgcgct tcatgctgat gaaaatgtcg aacggcttga cggcgggctt gaatccctcc
     2161 ggcacctgaa acgcctcgat ttccggttcg gcgctggacg tccgccggat ttcgtgacgg
     2221 cggatgtagt aaccgtagac cttgagcagg ccgagccccg tcatcatgcc gagaaaggga
     2281 ggcagatgca ggaattgatg caacgagacg gtcagcgcga tagtcaacag gaacagcgcg
     2341 atgacgacat agccgccgtg cttgacgcgg atacgctcgg tcgccgccgc gggtttgccg
     2401 cgcggcacgg agaaagccat gatcgccgcg ggcacgagcc aattcaccag ggacgggatg
     2461 aagaggtcga agaactcctc gaagcggacg acgccttttt gccagaccat gagtgtggtg
     2521 atatcgccga aaggactgaa ggccccgccg gcattggcac cgacgacgat gctgatgcag
     2581 gagaccgcga cgaagcgctt gttgtcgccg gccacggcta gcaccacggc ggccatgagc
     2641 agtgccgtgg tcaggttgtc cgcgaacggc gaaataaaaa aggcgaacag tccggcgagc
     2701 cagtaaatcg tgcgcagcga aaaaccggat gacaccatcc aggaccgcag cgcgcgaaaa
     2761 acgctgcgct cttccatggt attgatgtaa gtcatcgcgg aaagcaggaa taccatcagt
     2821 tccgcgtatt cgaggaaatt ggcgcgcagg gcgcgcgcgg cggtttcggt gtcgccgcgc
     2881 tgcgcatagg caatcgccac cagcacccag atgacgccgg cagccaccat catcggcttg
     2941 gacttgcgca ggagcgagaa ttcctcgctg ataacgaggc cgtaggccac cacaaaggtg
     3001 atcacggcca ggataccgag catacccgtc gtcagatcgg ggacggcgag cgtcccgctt
     3061 tccatggcgc atgcggttac aggcaacaag gccgtcaaca ccgccggcat ggcaaggctg
     3121 aagtgatgat ttttcatggt gacggttggt ggggcgctct gtttttccga ttattttgtc
     3181 gtgtctcccg ggagtctagc ggggcgcacc agactggaat agtgcgaata tcaacaaaac
     3241 attaagtttt aatatattga catatcaagt ccacggaggc attctggccg ccgttcgcaa
     3301 ctcggagcgt gatcggtcct ctctgttttt gcatgaaacc ggagtatgcc aacagcctgc
     3361 ctgacggctg ggatcgtttt tcccggttct ttttcgcgct cggcgacacc acccgccagc
     3421 aaatcctgct tttgttcgag cccggcgagg aaatctgcgt caacgacatc gcgcgcctgt
     3481 tcaagatcag ccgtccggcg atctcgcatc acctgaagat cctgcgcaac gccgaattgc
     3541 tggtgtgtga gaaacgcggc aaggaggttt actaccgcgt caatcacgat cactgcgccg
     3601 aggtcatgcg cgtggtgcgc gaattcatcg tgacccaggc gcctccctcc accctgactg
     3661 cttccgcctt ccgctgatcc agttcgtttt acgttccggg ccaatttcgg attgcaaatg
     3721 agccatgctc gctaggctca tccgtcttac cgtaaacatg tagccctgct cgctccgctc
     3781 cctcgtcttg acgtattatt acgacccagg tgacccaagc ccgagccgag aagtgcgcca
     3841 gggatggcgc gattacagag ccgaggatgc aagtatcctc tatatccatt cccccgacag
     3901 cgtcccggcg cgagattttc gcatgggcga tgtacgactt cgccaactcc ggttacacca
     3961 cggtggtgct cacggcggtg ttcaacgcct atttcgtcgg cgtcgtggcc ggtgacggcg
     4021 ccgacaagag cggcgcggcg acgctgctct ggaccgtggc catggccatc accaacatcc
     4081 tggtgctcgc gagcgcgccg gtgctgggcg ccatcgccga tcacggcgcg cacaagaaac
     4141 gctttcttgc gctgaccacc gttggctgcg tcctgttcac cgccttgctc ggattcgtcg
     4201 gccgcggcga cgtggtgctg ggcatgacct tggtgatact ggcgagtctc atgttcgcca
     4261 gcggcgaaaa tgtcatcgcc gcgtttctcc ccgagatcgc gagccagaag gacatgggcc
     4321 gcatatccgg ttacggctgg tcgcttggct atctcggcgg cctcctggtg ctgggcctgt
     4381 gcctcgcgta catccagtgg gcgcagtcgc gtggggagat cgcggaacag ttcgtgccgg
     4441 tgaccatgtg gatcacggcc gtcatgttcg cgcacgcggc gctgccgacg ttcgtctggc
     4501 tacgcgagcg cgccgtcccg caaccgctgg cgccgggcga gaactacatt cgcatcggct
     4561 tcgcgcgcgt gcggcacacg ttgacgcacg cgcgtcacta tcgcgacctg ttccgttttc
     4621 tcgtcacgct cgcggtgtat tactgcggca tcaacaccgt ggtggtgctg gccgcgatct
     4681 acgcgcagga gacgatgggc ttcaccatgc aggagaacat catcctgatt ctggtggtca
     4741 acgtgaccgc cgcggcgggt gcgctcacgt tcggtctggt gcaggatcgt ctcggctcgg
     4801 tgcgcacctt ggcaatcacg ctgacggtgt gggttgtggc gttggtgctc gcgtatttca
     4861 tcgaaagccg cgccggcttc tgggtggtgg cgaatctcgt cggactcgcg ctcggctcca
     4921 gccaatcggc cggccgcgcg ctggtgggac agttctcgcc gcccgaacgc gcggcggaat
     4981 tctttggcct gtggggactg gccggcaagc tcgccgccgt catcgggccg ttggtgtatg
     5041 gtctggtgac ttacctgacg cacggcaatc accgccaggc gctgctcgcc accacgacgt
     5101 ttttcatcgt cggcctgctg ctgttgatga ccgtcaacga acgccgcggg cgtgccgccg
     5161 cggttcatcc ggattgagtt tctcatgccg cccatgactg tcatcgcatt cctggcgctg
     5221 gcggccggcg ttgttctgct tgtatggctg tggctccgtt tgctgcgcgc ctgcgaggcc
     5281 gccaacggtg ccgactgggg tggccgctgg ctcaaccggc ttgacggatt gaaccgcatt
     5341 ttctgccgtc gttttcatag tctggtgtat gaaccgctga atctgcccgc cgctggtggt
     5401 gcgctcgtgg catccaacca cgtttccggt ctcgacccgc tgctgctcat tgccgccagc
     5461 ccgcggccgc tgcgttttct gatcgcgcgc gaggagtacg accgctggtg gctgcgctgg
     5521 ctgtttcgac gcatcggctg cattccggtg gagcgcacgc gcaatacccg cagcgcgttc
     5581 gaggcggcgc gcaaggcgct caagcgcggc gaggtggtgg cgctattccc gcacgggcgc
     5641 atccatctcg atcatcatga gccgatcccc ctgaagcgcg ggatcgcctt gctgtcgtcc
     5701 ctgacgggcg tgccgattca tccggtgcgc atcgagggag tgcgcgcgca gggcatgacg
     5761 gtcacggccg tattcgttcc cagccgcgcc tgtctcctga gctttgcgcc gctgcactgt
     5821 gccgggctgg aggacgaaca ttgtctggaa gaactgtggg accggctggc cctgtcaggc
     5881 gaggaacgga gacatgacaa cccgcaccgc cagcgccgca gcgggaaatc gccggacgat
     5941 ggcgaaaccg cgcagtaaca cttgaaaaag ggagactgtc gcccccattt aacagccaga
     6001 atgaaattcc tgaagggagg atgattactc catgcgcatg gacaaactga ccaccaaatt
     6061 ccagcaggca ctggccgacg cgcaaagcct ggcgctcgga cgcgaccacc agttcatcga
     6121 acccgcgcac ctgatgacgg ccttgctcga tcaggaaggc ggcaccacgc ggcatctcct
     6181 caccaaatcc gacgtcaacg tcaatgccct gcgcacgaag ctttcgcaga tactcgaccg
     6241 catgcccacg gtggaagggg cgggcggcga ggtgcacgtc tcgaacaatc tggcgaattt
     6301 gctcaatctc accgacaagt acgcgcaaaa acgcggcgat caatatattt cgagcgagtt
     6361 gttcctgctg gccgcgctgg aagacaaggg cgagttcggg cgcttgctga aggaggccgg
     6421 tgtttccaag aacggcatcg aaaaggccat tgagaccatg cgtggcgggc agaaggtgga
     6481 agaccagaat gccgaagaag gccgccaggc actggaaaaa tataccatcg atctcaccga
     6541 gcgcgccgag cagggcaagc tcgatccggt gatcgggcgc gacgacgaaa tccgccgctg
     6601 catccagatc ctgcaacgcc gcaccaagaa caatccggtg ctgatcggcg agcccggtgt
     6661 cggcaagacc gccatcgcgg aaggcctggc gcaacgcatc atcaacagcg aggtgccgga
     6721 agggctgaag ggcaagcgtc tgttggcgct cgacatgggc gcgctcatcg ccggcgccaa
     6781 gtttcgcggc gagttcgagg aacggttgaa ggccgtgctc aaggacctgt ccaagcagga
     6841 aggccgcatc attctgttta tcgatgagtt gcacaccatg gtcggcgccg gcaaggctga
     6901 aggcgccatg gatgtcggca acatgctgaa gcccgcgctg gcgcgcggcg aactgcattg
     6961 catcggcgcg accacactgg atgaataccg caaatacgtg gagaaagacg ctgcactcga
     7021 gcgtcgcttc cagaaggtgc tggtagacga accgacggtc gaggacacca tcgccatcct
     7081 gcgtggtctg aaagagaaat acgaagttca tcacggcgtg gacatcaccg acccggccat
     7141 tgtcgccgcg gccatgctgt cgcatcgcta catcaccgac cgccagttgc cggacaaggc
     7201 catcgatctg gtggacgagg cggcctcgcg catccgcatg gagatcgact ccaagcccga
     7261 gtccatggat cgtctcgatc gtcggctcat ccagctcaag atcgagcgtg aggcgctcaa
     7321 gaaagaaact gacgaggcct cgaagcagcg cttggccacg cttgaaggcg agatcaaaaa
     7381 actggaaaag gaatattccg acctggaaga ggtctggaaa tccgaaaagg ccgcggtgca
     7441 tggcgcgcaa catataaagg aggcgctcga ccgcgcccgg ctcgattttg aaaccgcgcg
     7501 tcgcgccggc gatctggcgc gcatgtcgga attgcaatac ggccgcatcc ccgaactgga
     7561 aaaacagctc aacgccgcgc aggccgccga aggcaaggaa cgcaagc
//
LOCUS       DBNGZE010000485         3828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1801886, whole genome shotgun sequence.
ACCESSION   DBNGZE010000485 DBNGZE010000000
VERSION     DBNGZE010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1801886"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="AC16FV_12530"
     CDS             <1..286
                     /locus_tag="AC16FV_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR2850911.1"
                     /translation="FVVRNGRRLGGRAVQARLQQWARRQGMEVPVHPHMLRHSFASHV
                     LESSGDLRAVQEMLGHANISTTQIYTHLDFQHLAGVYDKAHPRARKKTPA"
     gene            283..1140
                     /locus_tag="AC16FV_12535"
     CDS             283..1140
                     /locus_tag="AC16FV_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850912.1"
                     /translation="MSAPRYYSMRRLSPYQGTVQIVELPDFRAVTSDGIAWRVQFLNQ
                     RSRVSGHAVWRADGSGNLIETPYTSELIQALQNHPSFPFPSQDNLELWLLDAREQKPL
                     AIRASTLGRIRPPRSVDTRWHATLAEVDSFVAPSLAPENGAVSTSHIPHSQVLSRCVQ
                     KAAGPLERAQWFRREDDGSGTGLHGCRLDAAQVGRKLGRDAFPELLLREQWEVEQEQK
                     LVSDYHDWQAPNLLTHSRLAHATRDRLERAACRQAEKLYRVRHFLPEIINRDLLNVAF
                     VEAVIRRST"
     gene            1227..1859
                     /locus_tag="AC16FV_12540"
     CDS             1227..1859
                     /locus_tag="AC16FV_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR2850913.1"
                     /translation="MAAQSTRDKIIDTALDLAGRRSWESLRLHDVAAELRLDLNDVRA
                     HFREKEDIVDAWFDRADAAMLHAGTALGFVDLSARERLHRLIMAWLAALAPRRRVTRQ
                     MIYGKFEPGHVHYQFAGLLRVSRTVQWLREAAHRDAVLPWRAFEETGLTAIYLATFFC
                     WMRDVSENAARTSAFLDRLLGRAEHFARSWPGFSGSRAKAGAPPASTPKV"
     gene            1890..2261
                     /locus_tag="AC16FV_12545"
     CDS             1890..2261
                     /locus_tag="AC16FV_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR2850914.1"
                     /translation="MADTFKNIPIKADIGNQEHGEIRSCADAEPYALRVIGDSMEPEF
                     LDGHILIVDPAMPPQHGAYVIIDYQGETTFRQFILEDGKKYLKALNSAYPAVELVENY
                     SVRGVVVQRASRRRKDHKHYY"
     gene            complement(2295..3590)
                     /locus_tag="AC16FV_12550"
     CDS             complement(2295..3590)
                     /locus_tag="AC16FV_12550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2850915.1"
                     /translation="MEKDVHKSRLTSLNIPTLTSGRVETGLFRASSRVSVLDRWMARK
                     LLDLLGDPPLTFVLWNGEKIPSADKASVARVHFHDSEVLQKLLVNPALHFGDLYSAGR
                     IEVEGDLVQFLVLAYCAAASSPKYQKLKEAQTRFFNRPRANGLADSRDNIHHHYDIGN
                     DFYELWLDREAMQYTCAYFPSPDLTIEEAQRAKMDHVCRKLQLKPGDRVVEAGCGWGG
                     LARHMAQHYGVKVRSYNISHQQILYARERAQAAGLEESVEYVEDDYRNITGEYDVFVS
                     VGMLEHVGRDHYRELGDVIHRCLAPAGRGLIHSIGRNKPELMNAWIEKRIFPGAYPPT
                     LREMMDIFEEHEFSVLDVENLRLHYAKTLEHWLGRFNRHAEEIEKSYDSAFVRAWRLY
                     LAGSIAGFTAGTLQLFQIVFTHGTNNDLPWSRAYLYREL"
BASE COUNT          728 a         1151 c         1185 g          764 t
ORIGIN      
        1 gttcgtggtg cgcaacggca ggcgcctggg cgggcgcgcg gtgcaggcac ggctgcaaca
       61 gtgggcccgg cgccaaggca tggaagtgcc ggtgcatccg cacatgttgc gccattcatt
      121 cgccagccac gtactggaat cgagtggcga cctgcgcgcg gtgcaggaaa tgctgggtca
      181 cgccaatatc tcgaccaccc agatctacac ccatctcgat tttcagcacc tcgcaggcgt
      241 ctacgacaag gcccatccgc gggcgcgcaa gaaaactccg gcatgagcgc gccgcgttat
      301 tacagcatgc gccgtctttc gccctatcag ggcacggtgc agatcgtgga attaccggat
      361 ttccgggccg tgacctccga tggcatcgcc tggcgcgtgc agttcctcaa tcagcgttcg
      421 cgggtttccg gtcacgccgt gtggcgcgcc gacggcagcg gaaatctgat tgaaaccccg
      481 tacaccagcg agctgatcca ggccctgcag aatcatccgt cgtttccttt tccgtcgcag
      541 gataatcttg agttgtggct gctggatgcc cgggaacaga aaccgctggc gatccgggcc
      601 agcacgctcg gccgcatccg gccgccgcgt tccgtcgata cccgttggca cgccaccctg
      661 gcggaggtcg acagtttcgt cgcgccgagt ctggcgccgg aaaacggtgc agtctcaacc
      721 tcgcacatcc cgcacagcca ggtattgagc cggtgcgtgc agaaggcagc gggcccgctg
      781 gagcgggcgc aatggttccg ccgcgaggac gacggcagcg gcaccggact gcacggctgt
      841 cgtctcgatg ccgcgcaggt agggcgcaag ctcgggcgcg atgcctttcc tgaactgctg
      901 ctgcgtgagc aatgggaagt ggaacaggag caaaaactcg tcagcgatta tcacgactgg
      961 caggcgccca acctgctgac ccactcacgg cttgcgcacg ccacacgcga ccggctcgaa
     1021 cgcgccgcct gccgccaggc ggagaagctc tacagggttc ggcacttcct gccggagatc
     1081 atcaatcgcg acctgctgaa tgtggccttc gtcgaggccg tgatccggcg ttccacctag
     1141 accgagtagg tgaattcctc acgccgctta tcgtcatgcg gggaatgact tagaatgctt
     1201 catctgttca tgtggaggct ggcggcatgg cggcccaatc gacccgagac aaaatcatcg
     1261 ataccgcgct cgatctcgcc ggacgccgat cctgggaaag tctgcgcctg catgacgtgg
     1321 cggcggagct gcggctggat ctgaatgacg tgcgcgcgca tttccgcgaa aaggaagaca
     1381 ttgtcgacgc ctggttcgat cgcgcggacg cggccatgct gcatgcgggc accgcgcttg
     1441 gattcgtcga tctgtcggca cgtgagcgtc tgcaccggtt gatcatggcg tggctcgcgg
     1501 cgctggcgcc gcgccggcgc gtgacgcgcc agatgattta cggaaaattc gagccgggac
     1561 acgtgcatta ccagttcgcg ggactgttgc gcgtcagtcg cacggtgcaa tggctgcgcg
     1621 aggcggcgca ccgtgacgcc gtgttgccgt ggcgcgcctt cgaggagacc ggcctcacgg
     1681 cgatttatct cgcgacattt ttctgctgga tgcgcgacgt gtcggaaaat gccgcgcgca
     1741 cttcggcgtt tctcgacaga ttgctgggac gggcggaaca tttcgcgcga tcctggcccg
     1801 ggttttcggg gtcacgcgcc aaggccggcg cgccgccagc ttccacgccg aaggtttgag
     1861 cacgtttttt ttgatcggag gtccggccca tggccgatac attcaagaat attcccatca
     1921 aagccgatat cggcaatcag gaacacggtg agatcaggag ttgcgccgac gccgagcctt
     1981 atgccttgcg ggtcatcggc gacagcatgg aaccggaatt cctcgatgga cacatcctga
     2041 tcgtggaccc ggccatgccg ccacaacatg gcgcctacgt gatcatcgac tatcagggcg
     2101 agaccacgtt tcgccagttc attctcgaag acggaaaaaa atacctgaag gcgctgaaca
     2161 gtgcctatcc ggcagtggag ctggtggaga attactcggt gcgcggggtg gtggtgcagc
     2221 gcgccagccg ccggcgcaag gaccacaagc actattactg attatgagtc catgagtcgt
     2281 gaagcgagcc ttttttacaa ttcacgatac agataggcgc gcgaccacgg caggtcgttg
     2341 ttggtgccgt gcgtgaaaac gatctgaaag agttgcagcg tgccggcggt gaaaccggcg
     2401 attgaaccgg cgagatacag ccgccacgcg cgcacgaaag cgctgtcgta ggatttttcg
     2461 atttcttccg cgtgccggtt gaaacgcccc agccagtgct ccagcgtttt ggcgtagtgc
     2521 aggcgcaggt tctcgacatc gagcacggaa aactcgtgtt cctcgaagat gtccatcatc
     2581 tcgcgcagcg tgggtggata ggcgccgggg aaaatgcgtt tttcgatcca ggcattcatc
     2641 agctcgggtt tgttgcggcc gatggaatga atcagtccgc gtcccgcggg tgccagacag
     2701 cgatgaatga cgtcgcccag ctcgcggtaa tgatcgcgcc cgacgtgttc cagcatgccg
     2761 acagaaacga acacgtcgta ttcgcccgtg atattccggt agtcgtcttc cacgtattcc
     2821 acgctttcct caaggccggc ggcttgggcc cgctctctcg catacagaat ctgctggtgt
     2881 gagatgttgt aggacctgac tttgacaccg taatgctgcg ccatgtggcg cgcgagcccg
     2941 ccccacccac aaccggcttc caccacccgg tctcccggct tcagttgaag cttgcggcac
     3001 acgtgatcca tcttggcacg ctgcgcctcc tcgatcgtca ggtccgggga cgggaaatag
     3061 gcgcaggtgt actgcatggc ctcgcggtcc agccacagtt cgtagaaatc gttgccgatg
     3121 tcgtagtgat gatgaatatt gtcgcgcgaa tccgcgagcc cgttggcgcg cgggcggttg
     3181 aaaaaccgcg tctgtgcctc cttgagtttc tggtattttg gggacgaggc cgcggcgcaa
     3241 taggcaagca cgaggaactg cacgagatcg ccttccacct cgatacgccc ggcgctgtag
     3301 aggtcgccga agtgcaacgc gggattcacc agcagcttct gcagtacttc gctgtcatgg
     3361 aaatgcactc gggcgacaga ggctttgtcc gcggagggga ttttttcgcc attccacagc
     3421 acgaaggtca gcggcggatc gccaagcagg tcgagcagtt tgcgcgccat ccagcgatcc
     3481 agcaccgaaa ctcgcgatga ggcacggaac aggccggtct cgacccttcc gctcgtcaac
     3541 gtcggaatat tgagcgaagt cagcctggat ttgtgcacgt ctttttccat gggcccgttg
     3601 ctccttcagg acggctttta tcctacctcg cctccatctt cctttacaaa tgttgcacgc
     3661 tgcgcgcgct ttcaaacgcc ggctttactt ggcatattca cgctatgtgc cgcgcaggag
     3721 tatacagcga gcgcgatttg ggcttcggcg cgacggcacg tgcaaggcgg gataatgtgt
     3781 aaaatggcgg catgaaatac cggcggtatc catccatctt ctgtattc
//
LOCUS       DBNGZE010000486         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1526923, whole genome shotgun sequence.
ACCESSION   DBNGZE010000486 DBNGZE010000000
VERSION     DBNGZE010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1526923"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /gene="prmC"
                     /locus_tag="AC16FV_12555"
     CDS             <1..752
                     /gene="prmC"
                     /locus_tag="AC16FV_12555"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR2850916.1"
                     /translation="LMHVCGFDRSNLITRHETILTGEQQSKLESLLARRKRGEPVAYI
                     TGTREFWSMEFNVTPATLIPRPETELLVEKALEHIPRDTEWTIADLGTGSGAIALAIA
                     KERPRCRVVAIDNSSTALEVARSNAEKFGLTNIEFREGDWFTSLADATFDMIVSNPPY
                     IRAGDPHLTQGDVRFEPATALVSGNDGLDAIRHIALHAREFLRPGGWLLFEHGWDQAV
                     IVGELLRQHGYRDMVCHRDLAGHTRVAACRR"
     gene            773..1510
                     /locus_tag="AC16FV_12560"
     CDS             773..1510
                     /locus_tag="AC16FV_12560"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR2850917.1"
                     /translation="MDDKQLLRYSRHILLPQVDVRGQEKLLAARVLIIGLGGLGSPAA
                     MYLAASGVGHLVLVDHDKVDLTNLQRQIVHATERVGQDKVASAARGLDALNPGVRITT
                     INQKLEGGALDEQVRLADAVVDATDNFASRFIINAACVRAKKPLISGAVVRFEGQVAV
                     FTPGGPCYRCLYAEGGGPDEACATLGVLAPAAGIIGSVEAVETIKVLLGIGQTLAGRL
                     LLFDALAMEWREMKLRRDPACPVCGTP"
     gene            1507..>1695
                     /locus_tag="AC16FV_12565"
     CDS             1507..>1695
                     /locus_tag="AC16FV_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR2850918.1"
                     /translation="MNAVHLPRPIVNQLLHLAQKSPEEEICGLISRDSRGFRKCYPVP
                     NQAGDKKRFFALEPKAQID"
BASE COUNT          323 a          528 c          529 g          315 t
ORIGIN      
        1 tgctgatgca tgtgtgcgga tttgatcgca gcaacctgat cacacgccat gaaaccatct
       61 tgaccggcga acagcaaagc aaactggaaa gcctgctcgc ccggcgcaaa cgtggcgaac
      121 ctgtcgccta catcaccggt acgcgtgaat tctggtccat ggaatttaat gtcacgccgg
      181 cgacgctgat accacgcccg gaaacggaac tattggtgga aaaggcgctg gagcatattc
      241 cgcgcgacac ggaatggacc atcgccgatc tcggcaccgg cagcggcgcg attgcgctgg
      301 ccattgcaaa agagcgtcca cgctgccgtg tggttgcaat agacaattca tccaccgcgc
      361 tcgaagtcgc ccggtccaat gccgaaaaat tcggcctgac gaatattgaa tttcgggaag
      421 gcgactggtt tacctcgctc gccgacgcaa cgttcgacat gatcgtgagc aatccgccct
      481 acatccgcgc cggcgacccg catctgacac agggcgacgt gcgtttcgag ccggcaaccg
      541 cgctcgtctc cggcaacgac gggcttgatg ccatccggca tatcgcgctg catgcgcggg
      601 aatttctcag gcccggcggc tggctgttgt tcgagcacgg ctgggatcag gccgtgatcg
      661 tcggagagct tctgcgccaa catggttatc gtgatatggt ttgtcatcgg gatttggccg
      721 gacacacccg tgtcgcggcg tgccgccggt aagctgattc ggttctaccg cgatggacga
      781 caaacaactg ctgcgctaca gccggcacat cctgttgccg caggtcgatg tccgcggtca
      841 ggaaaaactg ctcgccgcgc gtgtcctgat catcggcctt ggcggcctcg ggtcgccggc
      901 ggcgatgtat ctcgccgcga gcggtgtggg tcatctcgtt ctggtggacc atgacaaagt
      961 agacctcacc aatctccagc gccagatcgt gcacgccacc gagcgcgtcg ggcaggacaa
     1021 ggtggcttcg gccgcgcgcg ggctcgatgc actcaacccc ggcgtgcgca tcacgacgat
     1081 caaccaaaag ctcgaaggcg gcgcgctcga tgaacaggtg cgtctggccg acgccgtggt
     1141 ggatgccacc gacaatttcg ccagccgctt tatcatcaac gccgcctgcg tgcgtgcgaa
     1201 gaaaccactg atctccggcg ccgtggttcg cttcgagggt caagtcgcgg tatttacccc
     1261 gggaggaccc tgctatcgtt gtctgtacgc cgaaggcggc ggtccggatg aggcctgcgc
     1321 cacgctcggc gtgctcgcgc cggccgccgg catcatcggc tcggtggagg cggttgaaac
     1381 catcaaggtg ctgctcggca tcggccagac gctggccggc cggctgctgc tgttcgatgc
     1441 gctcgccatg gaatggcgcg agatgaaatt gcgcagggac ccggcctgtc cggtgtgcgg
     1501 cacgccatga acgccgtgca cctgccgcgc ccgatcgtga atcagctgct gcatctggcg
     1561 cagaaatcgc cggaggagga aatttgcggc ctgatctcgc gcgatagtcg cggcttccgg
     1621 aagtgctacc ctgtacccaa tcaggccggg gataaaaagc gctttttcgc actcgagccc
     1681 aaggcgcaga tcgac
//
LOCUS       DBNGZE010000487         4626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1041585, whole genome shotgun sequence.
ACCESSION   DBNGZE010000487 DBNGZE010000000
VERSION     DBNGZE010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1041585"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /gene="dapD"
                     /locus_tag="AC16FV_12570"
     CDS             <1..167
                     /gene="dapD"
                     /locus_tag="AC16FV_12570"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290621.1"
                     /note="catalyzes the formation of
                     N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and
                     tetrahydrodipicolinate in the lysine biosynthetic pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR2850919.1"
                     /translation="GEVTYGRVPKGAVVVSGNLPAADGKYSLYCAVIVKQVDEKTRSK
                     VGINELLRDI"
     gene            449..1810
                     /gene="radA"
                     /locus_tag="AC16FV_12575"
     CDS             449..1810
                     /gene="radA"
                     /locus_tag="AC16FV_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR2850920.1"
                     /translation="MAKVKIKSVYTCNECGASSPKWVGQCPSCGAWNTLYETTIAPAA
                     HARAGRQYGAPAEVHNLAEVSPEKVYRHPTGISELDRVLGGGLVAGSVVLIGGDPGIG
                     KSTLLTQAMATIGQTAKVLYVSGEESSDQIALRAQRLGLRGDRLRLLTENQVETILAS
                     AQSEQPQVIVVDSIQTMFTEILQSAPGSVAQVRESAAQLVRYAKQTGTSLFLVGHVTK
                     EGTLAGPRVLEHMVDTVLYFEGDTGSAFRIIRAIKNRFGAVNEIGVFAMTETGLREVG
                     NPSSMFLSRQGDAVPGSIVLATQEGTRPLLVEVQALVDQSHLSNPRRVCVGLDTNRLA
                     MLLAVLHRHAGIATYDQDVFVNVVGGVRVLETAADVAILAATVSSLRNRPLGKDLVAF
                     GEVGLAGEVRPVQRGQERIREAAKLGFKRALVPAANMPKKGDAGIELLPVRRLTEALE
                     ILG"
     gene            complement(1920..2627)
                     /locus_tag="AC16FV_12580"
     CDS             complement(1920..2627)
                     /locus_tag="AC16FV_12580"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR2850921.1"
                     /translation="MAKIIIKFNNDVVDHIDLRQGDMKIGRKPGCEIHIDNLSVSGEH
                     ANIFTVGEDSFIQDLGSTNGTFINNKKITKHHLKNGDTVVIGKHSLIYFTDSVRASQP
                     PDEFAKTVIINPSRTVEPKPAAPAPTEAAAITLETKPKDAPKVEVDRRRVGAIFVLSG
                     LNSGKRIDLTRKITNLGSAGKRAGAITQTTEGFMLAPGADEVPKLNGRPIPSEGSKLK
                     NGDVIEVAGTRLQFYLK"
     gene            complement(2690..3514)
                     /locus_tag="AC16FV_12585"
     CDS             complement(2690..3514)
                     /locus_tag="AC16FV_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR2850922.1"
                     /translation="MAVLGASNAMSNLLITGLTDPGRMREQNEDNIAMAPEAGLVVVA
                     DGMGGHRAGEVASRLAVEAITRHVIDTLAEAGTTGNGSIEVALVRDAIQQANQAIYSH
                     ARANPEYAGMGSTVVVALFYDDKLCVGHVGDSRLYRFRDTILEQVTEDHSVVQELVSR
                     GLVTAEEARLSISKNLVTRALGIDPEVEADISEHDVYDDDIYMLCSDGVNDVLADGDI
                     EMMLTEHGHNLETIARQMVDTANDRGGPDNISVILVRARRGFMRDPEVLQKLREQV"
     gene            complement(3791..>4626)
                     /gene="dapE"
                     /locus_tag="AC16FV_12590"
     CDS             complement(3791..>4626)
                     /gene="dapE"
                     /locus_tag="AC16FV_12590"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR2850923.1"
                     /translation="DMKSSLAAFVTAIEDFLAKHPQHTGTIGLLITSDEEGPAVHGTV
                     KVMEWLGKQGIRIDHCIVGEPSCVKTLGDTIKIGRRGSLTGRLFVHGKQGHVAYPHLA
                     NNPIHKLAPALAELAATEWDQGNADFPPTSFQVSNIHGGTGAENVIPGRLEVVFNFRF
                     STAVTDAELRRRVEEILKRHGVEHQLQWILSGHPFLTRGTALIGAVRRAVKDELGIEA
                     EASTSGGTSDGRFIAPTGTEVVELGPLNRTIHQIDECIAVEDLARLARVYRRILENLL
                     V"
BASE COUNT          830 a         1363 c         1462 g          971 t
ORIGIN      
        1 ccggcgaggt gacctacggc cgggtgccca agggcgcggt ggtggtctcg ggcaacctgc
       61 ccgccgccga tggcaagtac agcctctatt gcgccgtgat cgtgaaacag gtggacgaaa
      121 agacgcgctc caaagtcggc attaacgaat tgctgcgcga tatttaaaaa aaaatttcac
      181 aggatttaca gggcgaagcg gggtagtcgc cgatttactt aacgtacatc ggcgccgctg
      241 agcgcccgca cagggaggtg cgggccgggg tttatcggag tttctgtcgc gacgccaggg
      301 agggcatgct cgccgtccat ggcgcagcca tggaggcttc gatacactcg gccccgccat
      361 ccgtggcggg gcgctcgccg ggacgaagac acgttaagtg tcttcgtctg tttccggctt
      421 gccctggtta atcctgtcta ttcgatttat ggccaaggtc aaaataaaat cggtctacac
      481 ctgcaacgag tgcggcgcga gttcgcccaa gtgggtcggg cagtgcccgt cctgcggcgc
      541 gtggaacacg ctttacgaaa ccaccatcgc ccccgccgcg cacgcacgcg ccgggcgcca
      601 gtacggcgcg cccgcggaag tgcataacct ggccgaagtc tcgccggaaa aggtttaccg
      661 gcatccaacc ggcatcagcg aactcgaccg cgtgttgggc ggcggtctcg tggccggatc
      721 ggtggtgctg atcggcggcg atccgggcat cggcaaatcc acgttgctga cgcaggccat
      781 ggccaccatc ggccagaccg caaaggtgct ctatgtcagc ggcgaggaat cctccgacca
      841 gatcgcgctg cgcgcgcaac gcctggggct ccggggcgat cgcctgcgcc tgctgacgga
      901 gaatcaggtc gagaccattc tcgcgagcgc tcagagcgag cagccgcaag tcatcgtggt
      961 ggattcgatc cagaccatgt tcaccgagat tcttcagtcg gcgcccggca gcgtggcgca
     1021 ggtgcgcgag tcggcggccc agctggtgcg ttacgccaag caaaccggca cctcgttgtt
     1081 tctcgtgggc cacgtgacca aggagggcac gctcgccggc ccgcgcgtgc tggagcacat
     1141 ggtcgatacg gtgctgtatt tcgagggcga tacaggcagc gcctttcgca tcattcgcgc
     1201 catcaagaac cgcttcggcg cggtcaacga gatcggcgtg ttcgccatga ccgagaccgg
     1261 cctgcgcgag gtgggcaatc cgtcctcgat gttcctgagc cggcagggcg atgcggtgcc
     1321 gggcagcatc gtgctggcga cgcaggaggg cacgcgtccg ctgttggtgg aagtgcaggc
     1381 gctggtggat cagagccatc tgagcaatcc gcgacgcgtg tgcgtggggc tggacaccaa
     1441 ccggctggcc atgctgctgg cggtattgca ccgtcacgcc ggcatagcga cctacgatca
     1501 ggacgtgttc gtcaacgtgg tcggtggcgt gcgcgtgctg gaaaccgcgg cggacgtggc
     1561 gattctggcc gccacggtgt cgagtctgcg taatcgcccc ttgggcaaag acctggtggc
     1621 cttcggcgaa gtggggctgg cgggggaagt gcgaccggtg cagcgcgggc aggagcgcat
     1681 ccgcgaagcg gccaagctcg gtttcaagcg cgcgctggtc ccggcggcca acatgcctaa
     1741 aaagggcgat gccggtattg agctgttacc ggttcgtcgt cttacagagg ctttggaaat
     1801 acttggataa aaaattcaca ggattaacag gatttttcag gattaatttc tggaatttta
     1861 gctttgttgg atttttatcc tgttaatcct gcttttaatc ccgtaaatcc tgtgaaattt
     1921 tattttaagt aaaactgaag ccgagtgccc gcgacctcga tcacgtcgcc gttcttgagt
     1981 ttcgagcctt ccgagggaat cggtcgcccg ttcaatttcg ggacttcatc cgcgcccggc
     2041 gccagcatga agccttccgt ggtttgtgtg atcgcgcccg cgcgcttgcc ggcactgcca
     2101 agattggtga ttttcctggt caggtcgatg cgcttgccgc tgttcaatcc gctcaacacg
     2161 aaaatcgcgc cgacacggcg ccggtccacc tcgaccttcg gcgcgtcctt cggtttcgtt
     2221 tccagagtaa tggcggctgc ttctgtcggt gcgggagccg cgggcttggg ttccaccgtg
     2281 cgcgatggat tgatgatgac ggtcttggcg aattcgtccg gcggctgact ggcgcgcacc
     2341 gaatcggtga agtagatcag cgagtgcttg ccgatgacga ccgtatcgcc attcttcaga
     2401 tgatgcttgg tgattttctt gttgttgatg aacgttccat tggtgctgcc caggtcctgg
     2461 atgaacgaat cctcgcccac ggtgaaaata ttggcatgct cgccgctcac ggaaagattg
     2521 tcgatatgaa tctcgcatcc cggcttgcgg ccgatcttca tgtcgccctg tctcaggtcg
     2581 atatgatcga ccacgtcgtt gttgaacttg ataatgatct ttgccatttc cgtaccccag
     2641 tccctaaatt caacagcaaa aaatgcgccc gaccgatgga caattcattc taaacctgtt
     2701 cccgcaattt ctgcaggact tccggatcgc gcatgaagcc gcgacgcgcg cgcaccagaa
     2761 tgacagaaat attgtccgga ccgccgcggt cgttggcggt atccaccatc tggcgcgcaa
     2821 tggtttcgag attgtgcccg tgctcggtaa gcatcatctc gatgtcgccg tccgccagca
     2881 cgtcgttcac gccgtcggaa caaagcatat agatgtcgtc gtcgtacacg tcgtgttcgc
     2941 tgatatcggc ctcgacctcg gggtcgatgc cgagcgcgcg cgtgaccagg ttcttgctga
     3001 tcgacaggcg tgcctcttcc gccgttacca ggccacggct gaccagttcc tgcaccaccg
     3061 agtgatcctc ggtgacctgt tccagaatgg tgtcgcggaa acggtagagg cgtgaatcgc
     3121 cgacgtggcc gacgcacaac ttgtcgtcgt agaagagggc gacgacgacg gtcgaaccca
     3181 tgccggcgta ttccgggttc gcgcgtgcgt gcgagtagat cgcctggttg gcctgctgga
     3241 tggcgtcgcg caccaacgcc acctcgatgc tgccgttgcc ggttgttcct gcctcggcca
     3301 gcgtgtcgat gacatgccgc gtgatggcct cgacggcgag ccggctggcg acctcgccgg
     3361 cacggtgacc gcccatgcca tcggcgacca cgaccagtcc ggcctcgggc gccatggcga
     3421 tgttgtcctc gttctgctcg cgcatccggc ccgggtcggt caggccggtg atcaggagat
     3481 tgctcatggc gttgctggcc ccgagaacgg ccattggcgc gccgggaggg ggcggtaaag
     3541 cactaggatt gccgtgcgtg gcaggtgggt gatcatgatc ggaggtccag cagcactatc
     3601 ccttaatgta tccatttttc cggttcggca tccgcatttt gtgcagtgcc gtgttattcc
     3661 aaaaagtata ggtgcttcct gcgccgcagc cggcacgttt ttaacacatc gaaatgctca
     3721 gattatatct agaatataca ccatgaatat ttaatatttg tatgattttt atggtaaaaa
     3781 ttttcacgat tcagaccagc agattttcca ggatacggcg atacacccgc gccagccggg
     3841 ccagatcctc caccgcgatg cactcgtcga tctgatggat cgtccggttg agtggcccga
     3901 gttcaaccac ttccgtgccg gtcggggcga tgaagcggcc gtccgaggtg ccgccgctgg
     3961 tggaggcctc ggcctcgatg cccaactcgt ccttcacggc ccggcgcacg gctccgatca
     4021 gggcggtgcc gcgcgtcagg aaggggtggc ccgacagaat ccactggagc tgatgttcca
     4081 ccccgtggcg cttgaggatt tcttccacgc gccggcgcag ctcggcgtcg gtgacggcgg
     4141 tggagaagcg gaaattgaac accacctcga gccggccggg gatgacgttt tccgccccgg
     4201 tcccgccgtg aatgttcgag acttggaagc tggtcggcgg aaaatcggcg ttgccctggt
     4261 cccattcggt ggcggccagc tccgccagcg ccggggccag cttgtggatg gggttgttgg
     4321 ccagatgcgg gtaggccacg tgcccctgct tgccgtgcac aaataggcgg ccggtcagcg
     4381 agccgcgccg gccgatcttg atggtgtcgc cgagggtttt gacgcaggag ggctcaccga
     4441 cgatgcagtg gtcgattcgg atgccctgtt tgccaagcca ctccatgacc ttcacggtgc
     4501 catgcaccgc cgggccttcc tcgtccgagg tgatcagcag gccgatagtg cccgtgtgtt
     4561 gcgggtgctt ggccaaaaaa tcctcgatcg ccgttacgaa cgccgccagc gaggatttca
     4621 tgtcgg
//
LOCUS       DBNGZE010000488         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_138191, whole genome shotgun sequence.
ACCESSION   DBNGZE010000488 DBNGZE010000000
VERSION     DBNGZE010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_138191"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="AC16FV_12595"
     CDS             <1..1354
                     /locus_tag="AC16FV_12595"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR2850924.1"
                     /translation="QYVQTYVEGFDQFAEHVKEFTPEEMEPICGIPADTLREVARSFA
                     RAENAIIFWGMGVSQHTHGTDNARCLIALSLICGQVGRPGTGLHPLRGQNNVQGASDA
                     GLIPMFFPDYKSVESPEMIAKYEKAWGKKLDPKRGKTVVEIMDAVHADEIKGMYIMGE
                     NPAMSDPDLNHARGALSHLEHLVVQDIFLTETCSYADVILPASAWPEKDGTVTNTNRQ
                     VQMGRKALPLPGDARQDLWIIQDLAQRMGEKWNYKHVSEVYTEMANLMPALDNLSWER
                     VERENHVTYPSDAPDKPGRDVVFDKGFPRPNGMGKLVAAKLTPPDEMPDHDYPFILTT
                     GRQLEHWHTGAMTRRATVLDALEPSACASVSRGTLEKLGLKPGGMLRVTTRRGEVELA
                     ARQDDAIPDGVIFIPFAFVEAAANILTNPALDPFGKIPEFKFCAAKVEPAELRQQAAE
                     "
BASE COUNT          320 a          496 c          481 g          281 t
ORIGIN      
        1 gcaatatgtg cagacctatg tcgaaggctt cgatcagttc gccgagcacg tgaaggagtt
       61 tacgccggag gagatggagc cgatctgcgg catccctgcc gacacgctgc gcgaagtggc
      121 gcgcagcttt gcgcgcgcgg aaaacgccat catcttctgg ggcatgggtg tgtcgcagca
      181 cactcacggc accgataacg cgcgctgcct gatcgcgctg tcgctgatct gcggccaggt
      241 cgggcgcccc ggcactggtc tgcatccgct gcgcgggcag aacaacgtgc agggcgcctc
      301 tgacgccggt ctcattccga tgttcttccc cgactacaag tcagtggaga gcccggagat
      361 gatcgccaaa tacgaaaagg cctggggcaa gaaactcgat cccaagcgcg gcaagaccgt
      421 ggtcgagatc atggacgctg tccacgccga cgagatcaag ggcatgtaca tcatgggcga
      481 aaacccagcc atgtccgatc ccgacttgaa ccacgcgcgc ggcgcgctct cccatctcga
      541 acatctggtg gtgcaggaca ttttcctcac cgagacctgc tcctatgccg acgtaatcct
      601 gccggcctcg gcctggcccg agaaagatgg caccgtcacc aacactaatc gccaggtgca
      661 gatgggtcgc aaagcgctgc cgctgccggg cgacgcgcga caggacttgt ggattatcca
      721 ggatctcgct cagcgcatgg gcgagaaatg gaattacaag cacgtcagcg aagtctacac
      781 cgagatggcg aacttaatgc ccgcgctcga caatctctcg tgggaacgcg tcgagcgcga
      841 gaatcacgtc acttatccgt cggacgcgcc cgacaaaccg ggccgcgacg tggtgttcga
      901 caagggcttc ccgcgcccga acggcatggg taagctggtg gctgccaagc tcacgccgcc
      961 ggacgagatg ccggaccacg actatccgtt catcctcacc accggccgcc agctcgagca
     1021 ttggcacacc ggcgcaatga cgcgccgcgc gaccgtgctc gacgcgctgg agccgagtgc
     1081 gtgcgcttcg gtgtcccgcg gcacacttga gaaactaggg ctcaagcccg gcggcatgct
     1141 gcgcgtcacc acgcgccgcg gcgaagtcga actggcagcg cgccaggacg atgccatccc
     1201 tgacggcgtg atattcattc cgttcgcctt cgtggaagcg gcggcgaaca ttctgaccaa
     1261 tcccgcgctc gatcccttcg gcaagattcc tgaattcaaa ttctgcgcgg cgaaagtgga
     1321 gccagcggag ttgaggcagc aagcagcaga gtagaggctt cacgatagct tgaaataatc
     1381 atggccgggc tcatcctggc catcgatatt gctgcgatca ttttgcggga ggttcccgtg
     1441 ggccacatct gcttataatc tgaagcgccc ctgcagcatt ccggtcatcg agtcgtgtcg
     1501 ccagagaacg tcgtccgttg cggcgttggc ataccgccgc cggggcctgt tgagctattc
     1561 ggattagact gcacgcgt
//
LOCUS       DBNGZE010000489         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1458401, whole genome shotgun sequence.
ACCESSION   DBNGZE010000489 DBNGZE010000000
VERSION     DBNGZE010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1458401"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC16FV_12600"
     CDS             <1..443
                     /locus_tag="AC16FV_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR2850925.1"
                     /translation="VHWFDLERFYQEARRVLKPDGMLAVWCYGLSKINPEIDKVVQHY
                     YANIVGSYWPPERHYIDEKYETIPFPFVELPTPEFHMKAEWDLNEFIGYLHTWSATQR
                     FEKKNEQNPLNMVHRLLVKTWGDPQTRQTVRWPLYLRIGRVSSL"
     gene            471..1052
                     /locus_tag="AC16FV_12605"
     CDS             471..1052
                     /locus_tag="AC16FV_12605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR2850926.1"
                     /translation="MSKKILVILGQPQRHSYGGALMLAYIEGARAAGAEVRELYLGDL
                     KFDPLATTSLAHLAELEPDLAKAQEAIKWADHLVFVYPIWWGTIPALLKGFIERVFLP
                     GFAVHFRENSPLWDKLLTGRSARLIVTLNTPSWIYRWYFGRPGHNTMKKTILEFCGIR
                     PVRIMEVGPMKNSTEARRKQWLEQVRALGASMA"
     gene            complement(1280..2347)
                     /locus_tag="AC16FV_12610"
     CDS             complement(1280..2347)
                     /locus_tag="AC16FV_12610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR2850927.1"
                     /translation="MHFDLFYELAVPDFAGRDELGVFHETLDELALAETCGFRTAWLV
                     EHHFMRGYSHSSAPDLVLAAASQRTQTLRLGHAIVPLPYHHPLQVAERLATLDVLSNG
                     RVEFGFGRGFSPQEYATFGVDMSHSRGRVEESLAIIRQAFTGMPVQYSGKHFKIENAE
                     VLPKHVQQPHPPLWMAAVSPESFELAARLGVGVLVGPFKPWFMVQSDIEHYRTAWKKH
                     QGDSEESPRVGMTLGLLCLEDGQRARELAKTHVTWFYRELLKQTAPVLERLHAGYEYY
                     KKMGTLKFLAEKTLNLAVLESLGMVVVGDPAHCRKKLEAYRDAGVDHLLCAISAGGLP
                     TEAAQASMRTLADHVIPHFAN"
     gene            2492..>2830
                     /locus_tag="AC16FV_12615"
     CDS             2492..>2830
                     /locus_tag="AC16FV_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HIR2850928.1"
                     /translation="MSNEKVYEIPADAARRAHIDSNKYKEMYERSVKDPDGFWAEQAK
                     AFVTWSKPWSKVSDWSYDAGNLHLKWFEGGKLNVSWNCLDRHLEKRGNQVAILWEGDD
                     PREDRKITYRE"
BASE COUNT          597 a          811 c          839 g          583 t
ORIGIN      
        1 cggtgcactg gttcgatttg gaacggtttt atcaggaggc gcggcgcgtg ctgaagcccg
       61 acggcatgct cgccgtctgg tgttacggct tgagcaagat caatcccgag atcgacaagg
      121 tcgtgcaaca ttattatgcg aacattgtcg ggtcttactg gccacccgag cgccattaca
      181 tcgacgagaa gtacgagact attccattcc cgttcgtcga gctgccgacg ccggagttcc
      241 atatgaaggc cgagtgggat ttgaacgagt tcatcggtta tcttcacact tggtcggcca
      301 cgcagcggtt cgagaaaaag aacgaacaga atccgctcaa tatggtgcat cggttgctgg
      361 tgaaaacctg gggcgacccg cagacgcgac agaccgtgcg ctggccgctt tatctgcgga
      421 ttggtagagt ctcttccctg taacagtcgg ggttggacgg aaaaggaatt atgtcgaaaa
      481 aaattctggt gatactcggc cagccgcagc gccacagtta cggcggcgcc ctgatgctgg
      541 cctatattga aggcgcgcgc gctgccggcg cggaagtcag ggaactgtat ctcggcgatc
      601 tcaagttcga tccactggcg accacaagtc tggcgcacct ggccgagctc gaacccgatc
      661 ttgccaaggc gcaggaggcg atcaaatggg ccgatcacct ggtgttcgtt taccccatct
      721 ggtggggcac gattccggcg ctactcaagg gattcatcga gcgtgtattt ctcccgggct
      781 ttgccgtgca tttccgcgag aattccccgc tgtgggacaa actgctcacc ggccgcagtg
      841 cgcgcctgat cgtcacgctc aacacgccgt catggattta ccgctggtat ttcggccggc
      901 ccggccataa caccatgaag aaaacgatcc tcgaattttg tggcatccgg ccggtgcgga
      961 tcatggaagt ggggccgatg aaaaactcca ccgaggcccg gcgcaagcaa tggttggagc
     1021 aggtgcgcgc gttgggtgcc agcatggcgt gaaatgattg tctcgcgcca tcaggaaaaa
     1081 tctagagaag ttttgtctgt tccttcccac agcgctcaca gcgcagaagt gtgaccagtt
     1141 ttccacgttt gacgtcgaat ttcttttcgg tcacgacctt ccacttatga aagccgcttt
     1201 tacacaaggt cttaccctcg gcctttttct gtaacgacgg tttcctgaat tttatgattt
     1261 cacccatgtt gtcagcgcgt cagttggcga aatggggaat cacgtggtcg gcgagggtgc
     1321 gcatcgaggc ttgcgccgcc tcggtcggaa gcccgccggc gctgatggcg cacagcaggt
     1381 gatccacgcc ggcgtcgcga taggcctcaa gctttttgcg acagtgggcg ggatcgccga
     1441 cgacgaccat gcccagcgat tccaacacgg ccagattcag ggttttttcc gccagaaatt
     1501 tcaatgtgcc catcttctta tagtattcgt agccggcatg cagccgctcc agcaccggcg
     1561 ccgtctgttt cagcagctca cggtaaaacc aggtgacatg tgttttggcc agttcacgcg
     1621 cgcgctgtcc gtcttccagg cacagcagtc ccagcgtcat gccgacccgc ggtgattctt
     1681 ccgaatcgcc ctgatgcttt ttccaggcgg tgcggtagtg ctcgatgtcc gactgcacca
     1741 tgaaccaggg tttgaacggc ccgaccagca cgccaacgcc aagtcgcgcc gccagttcga
     1801 aactctcggg gcttaccgcc gccatccaca gaggtgggtg cggttgctgc acatgcttcg
     1861 gcagcacttc cgcgttttct atcttgaaat gtttgccgct gtactggacc ggcatccccg
     1921 tgaaagcctg ccggataatg gccagcgatt cctccacacg tccgcggctg tggctcatgt
     1981 cgacaccgaa ggtggcgtac tcctgcggcg aaaagccgcg gccgaatccg aactcgacgc
     2041 gaccgtttga gagcacatcg agggtcgcca gccgctcggc cacctgaagc ggatggtgat
     2101 agggcagggg gacgatcgcg tgacccagtc gcagggtttg ggtccgttgt gaagcggcgg
     2161 ccagcacgag atcgggcgcg ctggaatgag aataaccgcg catgaagtgg tgttcgacca
     2221 accaggcggt gcggaaaccg caggtctcgg cgagtgccag ttcgtccagg gtttcatgaa
     2281 ataccccaag ttcgtcgcgc ccggcgaagt caggcaccgc caattcgtaa aaaagatcga
     2341 aatgcatacg tgttagctta ccgcacacgg attccatgcg cccaaggcga tttcaggtcc
     2401 ggatatccac cggcggccct gccagcttga gcaacggtcc ggatatgctg caaaattgcg
     2461 tggcagcagt tccccaggag gagaaagaac aatgtcaaac gagaaggtct acgaaatccc
     2521 ggccgacgcc gccaggcgcg cgcacatcga cagcaacaaa tacaaggaga tgtacgagcg
     2581 ttcggtcaag gaccccgatg gtttctgggc cgagcaggcc aaggccttcg ttacctggtc
     2641 caagccctgg agcaaggtca gcgactggag ctatgacgcc ggaaacctgc atctcaaatg
     2701 gttcgagggc ggcaagctta acgtttcctg gaattgcctc gaccgccatc ttgagaagcg
     2761 cggcaatcag gtggcgattc tgtgggaagg cgatgacccg cgggaagacc gcaagatcac
     2821 ttaccgcgag
//
LOCUS       DBNGZE010000490         2040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_138434, whole genome shotgun sequence.
ACCESSION   DBNGZE010000490 DBNGZE010000000
VERSION     DBNGZE010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_138434"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="AC16FV_12620"
     CDS             <1..173
                     /locus_tag="AC16FV_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR2850929.1"
                     /translation="DLAGRLHAGELVNKVASGVGGKGGGRPDMAQAGGTNPAGLQAAL
                     DGVPAWVEQNLK"
     gene            266..1498
                     /locus_tag="AC16FV_12625"
     CDS             266..1498
                     /locus_tag="AC16FV_12625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR2850930.1"
                     /translation="MALIVQKYGGTSVGSPERINAVAEKVARMRKNGDDLVVVVSAMS
                     GETDRLLKLAKSINADPPPRELDVLLATGEQTTIALLAMALENIGCPARSYTGHQVHI
                     RTDSVYGKARILDIDESRVRDDLNKGRVVVVAGFQGVDEEGNITTLGRGGSDTTGVAM
                     AAALKADECQIYTDVDGVYTTDPRIVPEAQRLDRITFEEMLELASLGSKVLQIRSVEF
                     AGKYRVPLRVLSSFAEGPGTLITDEENNMEAPVISGIAFNRDEAKLTVRGVPDKPGIA
                     AHILNEIGKANINVDMIVQNVGADGTTDFTFTVHRGEYKKAMEILKRVSQELKAREVS
                     GDDKIVKISVVGVGMRSHAGIASQMFKTLADEGINIQMISTSEIKISVVVDEKYLELG
                     VRALHKAFGLEKSNKSPV"
     gene            1807..2028
                     /gene="csrA"
                     /locus_tag="AC16FV_12630"
     CDS             1807..2028
                     /gene="csrA"
                     /locus_tag="AC16FV_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR2850931.1"
                     /translation="MLILTRRIGETLNIGDDVQVTVLGIKGNQVRIGVNAPKEIPVHR
                     EEIYERIKKEKESGQVHPMPGLAHGGGKG"
BASE COUNT          463 a          565 c          645 g          367 t
ORIGIN      
        1 aggaccttgc gggccggctg catgctggcg agctcgtcaa caaggtcgct tcgggtgtgg
       61 gcggcaaggg cggtggccgg ccggacatgg cgcaggcggg tggcaccaac ccggctggcc
      121 tccaggcggc actcgacggt gtaccggcgt gggtcgagca gaatcttaag tagttccggc
      181 gttttcttgc tcctttggac tgtttactcc cgctgaaaaa gcgggtttaa tttgcgccct
      241 ttgctgtttc cctgaaccgg aaatcatggc cctcattgtc cagaaatacg gcggcacctc
      301 cgtcggcagc cccgagcgca tcaacgccgt ggccgagaag gtcgcgcgca tgcgcaagaa
      361 cggggacgat ctcgtcgtgg tggtttcggc catgagcggc gagacagacc ggttgctgaa
      421 gctggccaag tccatcaacg ccgatccgcc gccacgcgag ctcgacgtgt tgctcgcgac
      481 cggtgagcag accacgatcg ccttgctggc catggcattg gagaacatcg gctgcccggc
      541 gcgttcctac accggccatc aggtgcatat ccgcaccgac agtgtctacg gcaaggcgcg
      601 cattctcgac atcgacgaat cgcgcgtgcg tgacgatctg aacaaaggac gtgtcgtggt
      661 ggtggccggt ttccagggtg tggatgaaga gggcaatatc accacgctgg ggcgcggcgg
      721 ttcggatacc acgggcgtag ccatggccgc ggcgctcaag gcggatgaat gccagatcta
      781 taccgacgtg gacggcgtgt acacgaccga tccgcgcatc gtacccgagg cccagcgact
      841 cgaccgcatc acgttcgagg aaatgctcga attggcgagt ctgggttcca aagtactcca
      901 aatacgttca gtcgagttcg ccggcaagta tcgggttccg ttgcgcgtgc tgtcctcctt
      961 cgcggaaggg ccgggcacgc tcattaccga tgaggaaaac aatatggaag cgccagtcat
     1021 ctcgggcatc gcgttcaacc gcgacgaggc caagcttacg gtccgcggcg tgccggacaa
     1081 gcccggcatc gccgcccaca tcctgaacga aattggcaag gccaatatca acgtcgacat
     1141 gatcgtgcag aacgtgggcg cggacggcac caccgacttc actttcacgg ttcaccgcgg
     1201 tgaatacaag aaggccatgg aaatcctgaa gcgtgtatcg caggaactga aggcgcgcga
     1261 ggtcagcggc gacgacaaga tcgtgaaaat ctccgtggtc ggcgtaggca tgcgctcgca
     1321 cgccggtatc gccagccaga tgttcaagac cctggcggat gaaggcatca acatccagat
     1381 gatctctacc tcggagatca agatttccgt ggtggtcgat gaaaagtacc tggaattggg
     1441 ggtacgcgcc ctgcacaagg ccttcggtct cgaaaaaagc aataaatccc cggtttgacc
     1501 ccctccaaag ctggcacaga agctgcatag atataggcag gtaggcgaat acagacggat
     1561 agacaggatt ctcctgtgat atccgcgagt tagcaatcgg tcgggtgggc aggaaggccc
     1621 caggcaggtt gcgggagacg ctgaaaatgc cgtagcgcct gccgccatgg gaaagtttgc
     1681 caaaaatttg tcactgaaga tgttcggcaa cacatgttga ggagccggtc aggcccaggg
     1741 gtgggtgacc gggcaaaggg aagggaacgt tacggagtca cgcaatactg aaaggagtgt
     1801 agaacaatgc tgatacttac gagacgcatt ggcgaaaccc tcaacattgg cgacgatgtt
     1861 caggtcaccg tgcttggtat caaaggcaac caggtacgaa tcggcgtaaa cgcgcccaaa
     1921 gagatccccg tgcatcgcga ggaaatctac gagcgcatca aaaaagagaa agaatcgggt
     1981 caagtccatc ccatgccggg tctggcgcat ggcggcggta aaggctgagc ccgcggggtt
//
LOCUS       DBNGZE010000491         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1596779, whole genome shotgun sequence.
ACCESSION   DBNGZE010000491 DBNGZE010000000
VERSION     DBNGZE010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1596779"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1152)
                     /locus_tag="AC16FV_12635"
     CDS             complement(121..1152)
                     /locus_tag="AC16FV_12635"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR2850932.1"
                     /translation="MNIYMIGTQRSGSNLLRLMLNELPRIAAPHPPHILQRLMSLVPN
                     YGDLEKRENFAQLVDDVCRLVELNPVPWEGVTLNRLDIQLRCRRHTLMAVYEGVYDAM
                     AKAWEARSWCCKSMANIQYLPQIEEHFRSPRYIYLYRDGRDVACSFRKAVVGEKHFYH
                     IAREWAAAQQLALGMRERIGPSRFISISYESLTREPELTMRLLCKFLGVRFNQKMLEF
                     YKSDEAKRAAESSDLWGNVAKPIMADNTRKFLRDANQEDIRIFESVAGDVLSALGYER
                     MYVPQGVKLVFSEEEIRHFDAENRRLKDEVLQRASADDLKRRDRQAGLIQEIRDRGQM
                     APPSMRAIA"
     gene            complement(1216..1291)
                     /locus_tag="AC16FV_12640"
     ncRNA           complement(1216..1291)
                     /ncRNA_class="other"
                     /locus_tag="AC16FV_12640"
                     /product="sX9 sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02228"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02228"
     gene            complement(1357..>1850)
                     /locus_tag="AC16FV_12645"
     CDS             complement(1357..>1850)
                     /locus_tag="AC16FV_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015193031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR2850933.1"
                     /translation="VFAGMSVMLFGGGYVFIPLIQEIVVTNQKWVTSQEFVDGIALGQ
                     ITPGPILVSAAFIGLKVAGLAGATVATAAIFAPPAVLMVVASNAFGRLQRASAIQAAL
                     RGVRPAVIGMIFAAAVVVGRTAVPVWYSIAIFAIALYALIRFRVEAVWIIPAAGVAGW
                     WLY"
BASE COUNT          372 a          577 c          555 g          346 t
ORIGIN      
        1 aacgcagatt tattatctag atgaacgcgg ataaaaaaag aaattctatt taagtaatct
       61 gcgtttatcc aaattatcat ctgcgtccat ctgcggatac cttgttttta agattttgat
      121 tcaggcgatg gcgcgcatgg acggcggcgc catctggccg cggtcgcgga tctcctgaat
      181 cagtccggcc tggcggtcac ggcgcttgag gtcgtccgcg ctggcgcgct gcaatacctc
      241 gtccttgagc ctccggttct ccgcgtcgaa atggcggatt tcctcttcgc tgaaaaccag
      301 tttgacaccc tgcggcacat acatgcgctc gtaaccgagg gcgctcagca cgtcgcccgc
      361 cacggactcg aagatgcgta tgtcctcctg gttcgcgtcg cgcaggaact tgcgggtgtt
      421 gtcggccatg atcggcttgg ccacgttgcc ccacaggtcg ctcgattcgg ccgcgcgctt
      481 ggcctcgtcc gacttgtaga actccagcat cttctggttg aaacgcacgc ccaggaactt
      541 gcacagcagg cgcatggtga gctccggctc gcgggtcagc gattcgtaac tgatgctgat
      601 gaaacggctg ggcccgatgc gttcgcgcat gccgagcgcg agttgctggg ccgcggccca
      661 ttccctggcg atatggtaga aatgcttctc gcccaccacc gccttgcgga acgagcaagc
      721 cacgtcgcgt ccgtcgcgat acagatagat gtaacgcggg ctgcggaagt gttcctcgat
      781 ctgcggcagg tactggatgt tggccatgct cttgcagcac cagctgcgcg cctcccaggc
      841 cttggccatc gcgtcataca ctccctcgta cacggccatg agcgtgtgcc ggcggcagcg
      901 caactggatg tcgaggcgat tgagcgtcac gccctcccag ggcaccggat tgagctcgac
      961 caggcggcac acgtcgtcga ccagttgcgc gaaattctcg cgtttctcga ggtcaccgta
     1021 gttcggcacc agcgacatca gacgctgcag gatatgcggc ggatgcggcg cggcgatgcg
     1081 gggcaactcg ttcaacatca ggcgcagcag attcgagccc gagcgttggg ttccgatcat
     1141 gtagatgttc atggcaatca ctcctgaatc actgtttaaa caatatgcgc caagtggcac
     1201 agttccctag aagctgtctc aaaaacgtcg caagcggccg tcagccgggt gaggacggag
     1261 cgcaggaacc ggagtggaca cggcggtcca tgaggattcc gagcaccgcc cgagcccgga
     1321 tcacggacgc gcagcagttt ttgagatagc ttctactcaa tacagccacc aacccgccac
     1381 acccgcggcc gggatgatcc acaccgcctc cacgcgaaaa cgtatcagcg catacaacgc
     1441 gatcgcgaaa atcgcgatcg aataccacac cgggactgcg gtccgtccga cgaccacggc
     1501 ggcggcgaag atcatgccga tcaccgctgg acgcacgccg cgcagcgccg cctgaatcgc
     1561 gctggcgcgc tgcaggcgcc cgaaggcgtt ggaggccacg accatgagca cggccggggg
     1621 cgcgaagatg gcggcggtgg caactgtcgc gccggccaat cccgcgacct tgagtccgat
     1681 gaatgccgcg ctcaccagga tcgggccggg cgtgatctgc ccgagcgcga tgccatcgac
     1741 gaactcctgc gaagtgaccc atttctggtt ggtcaccacg atttcctgaa tcaggggaat
     1801 gaacacgtaa ccgccgccga agagcatgac actcatgccg gcgaacacgg
//
LOCUS       DBNGZE010000492         2427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_138571, whole genome shotgun sequence.
ACCESSION   DBNGZE010000492 DBNGZE010000000
VERSION     DBNGZE010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_138571"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16FV_12650"
     CDS             complement(<1..473)
                     /locus_tag="AC16FV_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR2850934.1"
                     /translation="MKAIVMTAPGGPEVLELRDLPEPQLTRDTDMLVRVKAAGVNPVD
                     TKMRAKPAAYAAPLPPVLGCDGAGIVQAVGNKAVTGFKPGDEVYYSQAPHHDRLGSYA
                     QYALVDHRLAARKPKRMSFTDAAAVPLVLITAWEALHDRAGIKQWHKVLIHAGAGG"
     gene            666..1085
                     /locus_tag="AC16FV_12655"
     CDS             666..1085
                     /locus_tag="AC16FV_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR2850935.1"
                     /translation="MATISEKVERQVVLLDENKTAWDAAVLMCDKHIGSVVVMRKSQV
                     IGLFTERDLIHHVILERCNPDTVVIKNVMRTKVPMVAPDETGEKCIELMKQHKCRHLL
                     VYDVDAFVGIISLRDLLVLLLEEKEELIRQLTKYVTS"
     gene            complement(1093..1839)
                     /locus_tag="AC16FV_12660"
     CDS             complement(1093..1839)
                     /locus_tag="AC16FV_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HIR2850936.1"
                     /translation="MNSTQRAILIVFPAVAALAAVLLLPPIAQDPAYHDFADRRGLLG
                     IPAFLNVVTNLAFLAVGVAGIIFCAARKGLSARYAWMTCFIGVALVSLGSGYYHLAPD
                     NGTLVWDRLPMSIGFMALSVAVLADYVNPRLEKILLAPAIILGLGSVIYWHYTDDLRL
                     YVLVQFLPLLMIPPVMLLFGATRKDRGYLLSAVATYMVSKLAEYYDRAVFERTGEIVS
                     GHSLKHLLAALALFAVYWMLRRRAPRSRPV"
     gene            1952..2227
                     /locus_tag="AC16FV_12665"
     CDS             1952..2227
                     /locus_tag="AC16FV_12665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR2850937.1"
                     /translation="MERRVQERQEVSLTVEVLDPPHLGKLKLRTRDLSKQGAFLLLKK
                     ELCLPVGRVVSLRMPGILWGEETSTISARVVRVTDEGMGLQFLDFDF"
BASE COUNT          490 a          729 c          743 g          465 t
ORIGIN      
        1 ccgcccgcgc cggcatgaat cagcaccttg tgccactgct tgatgccggc gcggtcgtgc
       61 agcgcctccc acgcggtgat tagcaccagc ggcaccgcgg cggcgtcggt gaagctcatg
      121 cgtttcggtt ttcgcgccgc caggcggtga tccaccagcg cgtattgcgc gtaactgccg
      181 agccggtcgt gatgcggcgc ctggctgtaa tacacctcgt cgccgggttt gaagccggtg
      241 acggccttgt tgcccacggc ctgcacgatg ccggcgccat cgcagcccag caccggcggc
      301 agcggcgctg catacgccgc gggtttggcg cgcatcttgg tgtccaccgg gttcacgccc
      361 gcggccttca cgcgcaccag catgtcggtg tcgcgcgtga gttgcggttc gggcaggtcc
      421 cgaagctcca atacttccgg cccgccaggc gcggtcatga cgatggcttt catagtcggg
      481 ttctccgtct caaaaatggc ttcaagccgc tgataatgcg ccattcccgc cgggcatcaa
      541 gacggtctcg ccgtcccggc ggagcttgtg cctataatct acgcctgagg cggctgtttt
      601 gggccggtgc ggcttcaagg ggccacacgt ccatccacgc tgttccgcga aaggagggct
      661 tcaccatggc cacaatcagc gaaaaggtgg aacgccaggt ggtgttgctg gatgaaaaca
      721 agaccgcttg ggatgccgcg gtgctcatgt gcgacaagca tatcggctcg gtagtggtga
      781 tgcgcaagtc ccaggtcatc ggcctcttca ccgagcgcga cctgatacac catgtcatcc
      841 tcgaacgctg caacccggac acggttgtga tcaagaacgt catgcgcacg aaggtgccga
      901 tggtagcgcc ggatgaaacc ggtgaaaaat gcatcgaact gatgaagcag cacaagtgcc
      961 ggcatctcct ggtgtacgac gttgatgcct tcgtcgggat catttcgctg cgcgacctgc
     1021 tggtgctgtt gctggaggaa aaagaggagc tgatccgcca gctcacgaaa tacgtcacca
     1081 gctgacggcg gattacaccg gccgggagcg cggggcgcga cgccgcaaca tccaatagac
     1141 cgcaaaaagc gcgagcgccg cgagcaggtg cttgagcgaa tgcccgctga cgatttctcc
     1201 agttctctcg aataccgccc ggtcgtaata ctcggccagc tttgaaacca tgtaggtcgc
     1261 caccgccgag agaagataac cgcggtcttt gcgcgtggca ccgaacagaa gcattaccgg
     1321 tggaatcatg agcaggggca ggaactgcac cagcacatag agccgcaaat cgtcggtgta
     1381 atgccagtag attacgctgc caagcccgag aatgattgca ggtgcgagca ggattttttc
     1441 cagccgtgga ttcacatagt cggccagcac cgcgaccgac agcgccatga aaccgatgct
     1501 catcggtagc cggtcccaca ccagcgtgcc gttgtccggc gccaggtgat agtacccgga
     1561 gcccagcgac accaacgcca cgccgatgaa gcaggtcatc catgcgtacc gcgcactgag
     1621 gcctttccgc gctgcacaaa aaatgattcc cgcgacgcct acggcgagga acgcgaggtt
     1681 ggtgacgaca ttgagaaatg ccgggatgcc gagcagaccg cggcggtcgg caaagtcgtg
     1741 gtacgctggg tcctgcgcga tcggcggcag cagcaggaca gccgccagcg cggcgaccgc
     1801 cggaaaaaca atcaggatcg cccgctgagt ggaattaatg acttagcctc catttataaa
     1861 attcttcccg ataatcaagg ccgtttaact agaaattccc ctgaagcctg ttaaggttaa
     1921 acgatacgtc gacaataaga aggggggcgt tttggaaagg cgtgttcagg agcgtcaaga
     1981 ggtatcgctt accgttgaag tgctcgatcc accgcatctc ggcaagctta agctccgcac
     2041 ccgcgacctc agcaaacagg gcgcgtttct gcttctgaaa aaagaactgt gcctgcccgt
     2101 tggaagggtg gtgtccttgc gcatgcccgg cattttatgg ggcgaggaga cgtccaccat
     2161 ctcggcccgg gtggtgcgcg tcaccgacga aggcatgggt ttgcagtttc tcgacttcga
     2221 tttctaagga agcttactga ctcggagctt ccccggggct gtccgacggc gttagccttt
     2281 cttgccccgg agttgtttgg acgaatcgct caatctcagt tgagaacctt gtagcccctg
     2341 ttgcgcaggc agtttttgat cacgagttgt cgctcctgcc gggtggcggt ggcgccgcgc
     2401 gccacgcctg tcacggcccc gacgccc
//
LOCUS       DBNGZE010000493         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_68910, whole genome shotgun sequence.
ACCESSION   DBNGZE010000493 DBNGZE010000000
VERSION     DBNGZE010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_68910"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC16FV_12670"
     CDS             <1..455
                     /locus_tag="AC16FV_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR2850938.1"
                     /translation="ATLHTNDAVGAVTRLVDMGVEPFLVASSLLGVLAQRLVRKLCPV
                     CRREHTPDGAELKMLGNGHDASGAKSIYIPGSCPACANTGYQGRTGIYELLSVDDSMR
                     TLIHDGASEGKLRDYARARGMRTRRDDGLRWVRAGATSLEEVLRVTRE"
     gene            458..1678
                     /gene="gspF"
                     /locus_tag="AC16FV_12675"
     CDS             458..1678
                     /gene="gspF"
                     /locus_tag="AC16FV_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR2850939.1"
                     /translation="MAAFEYQALDAMGHAVKGVMEGDAERQVRAALREKGLTPLQVEV
                     IDQRAESRRGGWHFSLRRGIPGSELALITRQFATLIHAGLTIEECLNALIEQTESARS
                     RAVLAGVRTRLLEGQSLARSLAQFPDSFPDIFRSMVDAGEQSGRLSEVLERLADYTEN
                     RQSLQQKVLLAFIYPALITLVASAVVIGLVVYVVPQVTRVFENTGQKLPIITEVLITI
                     SNFLRAGGWWWLIAAVAAFLAFRVALREPHLRLRWHHLLLHMPLVGRLLRGTNAARLA
                     STLGILTASGIPLLNAMQSAVQTVTNLPMRAAVEDALRQVREGGSLSRALGKTKLFPP
                     MVIHLIASGEATGKLDQMLERAATAQTRELENWVRALTALLEPVLILVMGAIVLFIVL
                     AILLPIFEMNQLIK"
     gene            1708..2136
                     /gene="gspG"
                     /locus_tag="AC16FV_12680"
     CDS             1708..2136
                     /gene="gspG"
                     /locus_tag="AC16FV_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR2850940.1"
                     /translation="MNTVRGFTLIEVLVVVVILSILAALIVPRIMDRPDQARIIAAKS
                     DIQAIANALKLYRLDNGVYPTTEQGLQALNKKPETGEIPRNWKSSGYLDRLPKDPWKN
                     DYQYLNPGLHGEIDVYSLGADGQPGGDGINADIGTWNIDY"
     gene            2146..2643
                     /locus_tag="AC16FV_12685"
     CDS             2146..2643
                     /locus_tag="AC16FV_12685"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR2850941.1"
                     /translation="MNSPRAQQGFTLIEVAVVMLVIVIVLGIVSVNLEPDRETPVRDE
                     ARRLALLLQTAQQEAILQGKVLAVTIERQGYYFLMLNDKSEFKPMDDDEVLHARPLPA
                     GIVISAVDIEGLPENEKPRLLLLPTGELPAFTVTFSQGDIRWQVEGTLTGEVTAQVAP
                     LPGKA"
     gene            2640..3026
                     /gene="gspI"
                     /locus_tag="AC16FV_12690"
     CDS             2640..3026
                     /gene="gspI"
                     /locus_tag="AC16FV_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HIR2850942.1"
                     /translation="MTHRGFTLVEVLIALAVLAIALTAVLRTMGQAIDSTNTLRERST
                     ALWVAQNRLAEHQMRRDWPAPDTTDGEAEIGGVKWFWREQVSTTPESRIRRIEITVRR
                     IAGSPDTQARLVGYLRNTIEAKKTGT"
BASE COUNT          614 a          920 c          933 g          592 t
ORIGIN      
        1 tcgctaccct gcacaccaac gacgccgtgg gtgccgtgac acgtcttgtg gacatgggtg
       61 tggagccgtt tctggtggca tcgagcctgc tcggcgtcct ggcccagcgt ttggtacgca
      121 agctctgtcc ggtgtgtcgc cgcgaacaca cgcccgatgg ggcggaactc aaaatgctcg
      181 gtaatggtca cgacgcatcc ggggcaaaat ccatatacat acctggaagc tgcccagcct
      241 gcgccaacac cggataccag ggccggaccg gcatttatga actgttgagc gttgacgaca
      301 gcatgcgcac tctcattcat gacggcgctt ccgagggcaa attgcgcgac tatgcccgtg
      361 cgcgtggcat gcgcacccgt cgcgatgacg ggctgcgctg ggtgcgcgcg ggcgcgacca
      421 gccttgaaga agtactgcgc gtgacgcgcg aataatcatg gcggcgtttg aataccaggc
      481 actggatgcc atgggtcacg ccgtcaaggg cgtgatggaa ggtgatgcgg agcggcaggt
      541 gcgcgctgcg ctacgcgaaa aagggctgac gccgctgcag gtcgaggtta tcgaccagcg
      601 cgccgagtcc aggcgcggtg gctggcactt ttccctgcgg cgtggcattc ccggttccga
      661 actggcattg atcacgcgcc agtttgccac cctgattcat gcgggcctga ccatcgagga
      721 atgcctcaac gccctcatcg aacagaccga atctgcgcgt tcccgcgccg tgttggccgg
      781 ggtgcgcacg cgcctgctgg aaggccagtc gctggcgcgc agcctggcgc agtttcccga
      841 ctccttcccg gacattttcc gcagcatggt ggacgccggc gagcaatcgg gccggctcag
      901 cgaagtgctc gagcgcttag cggattacac tgaaaaccgg caatcactcc agcagaaggt
      961 tctgctggcg ttcatctacc cggcgctgat taccctggtc gccagcgcgg ttgtcatcgg
     1021 tctggtcgtc tatgtcgtgc cacaagtcac gcgcgttttc gaaaacaccg gacaaaagct
     1081 gccgatcatc acggaagtgc tgattaccat cagcaatttt ctgcgcgctg gcgggtggtg
     1141 gtggctgatc gccgccgtgg ccgcattcct cgccttccgt gtcgccttgc gtgaaccaca
     1201 tctgcgtctg cgctggcatc atctgctgtt gcacatgccg ctggtggggc gcctgttgcg
     1261 cggcaccaac gcggcgcgct tggccagtac gctcggtatt ctgacggcaa gcggcatacc
     1321 actgctcaac gccatgcagt cggcggtgca gaccgtcacc aatctcccca tgcgcgccgc
     1381 cgtggaagat gcgttgcgtc aggtacgtga aggcggaagc ctttcgcgcg cactcggcaa
     1441 aaccaagctc tttccaccca tggtgataca tctgatcgcc agcggcgaag ccaccggcaa
     1501 actcgaccag atgctggaac gcgccgccac ggcgcagaca cgggagctgg aaaactgggt
     1561 gcgggcgctg acagccctgc tcgagccggt gctcattctg gtgatgggcg cgatcgtgtt
     1621 attcatcgtg ctcgccattc tgttgccaat tttcgaaatg aatcagctca tcaaataaag
     1681 aggatgttta tgaagcatac ggtccgtatg aatacagtca ggggcttcac gctcatcgaa
     1741 gttctggttg tcgtggttat cctcagcatc ctcgcggcat tgattgttcc tcgcatcatg
     1801 gaccgcccgg atcaggcacg catcatcgcc gccaaaagcg acattcaggc catcgccaat
     1861 gcgctcaagc tgtatcgcct ggacaacggg gtttatccca cgactgagca gggtttacag
     1921 gcgctcaaca aaaagccgga aaccggcgag attccgcgca actggaagtc cagcggctat
     1981 ctggatcgtc tgcccaagga tccgtggaaa aacgattacc agtatctgaa tccgggactg
     2041 catggcgaga ttgacgtata cagtctgggt gccgacggtc agcccggcgg cgatggcatc
     2101 aacgccgaca tcggaacgtg gaatatcgat tactgagtcc ggacaatgaa ttccccgcgc
     2161 gcgcaacagg gctttacgct gatcgaggtt gcggtggtga tgctggtaat cgtcatcgtt
     2221 ctcggcatcg tcagcgtcaa cctcgaaccc gaccgggaaa caccggtgcg cgacgaggcc
     2281 cggcgcctgg cattgttgct gcaaaccgcg cagcaggaag ccatcctgca gggaaaggtt
     2341 ctggcggtga ccatcgagcg tcagggttat tatttcctga tgttgaacga taaaagtgaa
     2401 ttcaagccga tggacgacga tgaggtgctg cacgcccgtc cactgccagc cggtatcgtc
     2461 atttcagccg tcgatatcga aggtctgccg gaaaatgaaa aaccgcgttt gcttctgctg
     2521 cctaccggtg agctgcctgc gttcaccgtt accttctcgc agggtgatat ccgctggcag
     2581 gtggagggaa ccctgacggg ggaagtcacg gcacaagtcg ctccgcttcc gggaaaggca
     2641 tgacgcaccg cggcttcacg ctggtggaag tgctgatcgc gctcgccgtg ctggcgatcg
     2701 cgctgaccgc cgtcctgcgc accatgggtc aggccatcga ctccacgaac acgctgcgcg
     2761 aacgcagcac cgcgctctgg gtggcgcaaa accggctggc cgaacaccag atgcgtcgcg
     2821 actggccggc tcccgatacg actgatggcg aagccgagat cggcggagtg aaatggttct
     2881 ggcgcgagca agtgtccacc acgccggaat cgagaatccg gcgcattgaa atcaccgtgc
     2941 gacgcatcgc cggcagcccg gacacgcaag ccaggctggt gggttatctg cgcaatacca
     3001 ttgaggcaaa aaaaaccggt acatgaaata caagcgcggt ttcacccttc tcgaactcc
//
LOCUS       DBNGZE010000494         3760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_207993, whole genome shotgun sequence.
ACCESSION   DBNGZE010000494 DBNGZE010000000
VERSION     DBNGZE010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_207993"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="AC16FV_12695"
     CDS             complement(<1..614)
                     /locus_tag="AC16FV_12695"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR2850943.1"
                     /translation="MRQTIKTLIVVPENNTTMEPEISALCPALAPIPVARVKRPARTL
                     LLEDLPTYAEATLDAIEPFAAEQFDLVIYGCTAAGFLGGPSGNARMVDRLRERTCATV
                     VSTAGAMVEALRSAGVSNTAVVTPYLKPVNDGLRDYLESSGIHVETLNSFFCKTTAEL
                     GQITEDQVLELARRTVTARSKSLFVACSQLPTLNVVAQLRAELQ"
     gene            complement(637..1392)
                     /locus_tag="AC16FV_12700"
     CDS             complement(637..1392)
                     /locus_tag="AC16FV_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2850944.1"
                     /translation="MKINDYDLDIVVQGFPGKSVCHGGLGWSSVVLLRAHDRIVLIDT
                     GGFSMRTMLIKRFAERGLKPADITDLLLTHSHHDHSVNWTMFPKARIVIGANELEWSL
                     KAPWGIGPVPELYVRELNTWPTVHKAADGEEVFPGITAHLAPGHTPGCLVFVLHAGER
                     DVIFAGDAAKNRAEIVSGTTDMTYDAAVSKASIEAIWKLWRKRPGNILVPGHDVPMML
                     EDGKPEYIDKREAAIRVWYGDDIETTTLFKLTV"
     gene            complement(1395..2507)
                     /locus_tag="AC16FV_12705"
     CDS             complement(1395..2507)
                     /locus_tag="AC16FV_12705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR2850945.1"
                     /translation="MSTVSEATKPETMQGPFYAAVEAAEAFGRRLLVAHGLSEQDAAI
                     MAGCLVRADLRGVDTHGLQFLPQYLDRVRRGLVNQKPNLVVECVTPMIGSLDGQNGYG
                     FVVATKAMAAAIEMAKEFGVGIVSARRSTHFGMAACYTLQALEAGLISFVFTNASRAM
                     PPWGGRDAFLGTSPFAAAAPAGKEVPFDLDMSPAVAARGKIRRAARRGQKIPIGYALN
                     KNGQPTTDPNAALDGGVVLPIGGPKGSGISMLMDVMSGVISGAAYGGDVRNQFLDYDQ
                     PQNVGHFFMAMKPDLFISKDEYLERMDTLVQRVHAVPTAESFDEVIMPGETERRLEAS
                     RRRTGLPYHAKEIAALQKEAATAGLPALPVSNAPLV"
     gene            complement(2519..3520)
                     /locus_tag="AC16FV_12710"
     CDS             complement(2519..3520)
                     /locus_tag="AC16FV_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2850946.1"
                     /translation="MWRKLGAASLCAAALGVAAHAEVNEVRMSKQFGLPYLPMIVIEA
                     QHLIEKNAKAAGLGDVKTSWTQRVGPAAVLDALLAGQADFIGPGATTLATIWEKTVGT
                     PQEVRALIAMQSMPFVLVTRNPDVKTIADFTDNDKIALPAVKLTGHALILEMEAAKIW
                     GADHYDKLDSITITRSHADAAAAVMGGGSEINSHFASSPFYYYELATPGVHQVLKSYD
                     VVGGKHTNGTLVATKKFHDANPKICAAVVAAFNDANAFIKAHPREAAQTYLTATKDKN
                     PLDALEKMVADPDVEYTTTPVNVMKFVDFMYKVGRLKKKPASWKDMFFPEAHGLNGS"
     gene            complement(3548..>3760)
                     /locus_tag="AC16FV_12715"
     CDS             complement(3548..>3760)
                     /locus_tag="AC16FV_12715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR2850947.1"
                     /translation="ARAMIAANPDRIVWGTDWPHPGAAKRDPAVIEPFRPEDDGAALN
                     RLAGWTKTRTELQKILVDNPAGLYQF"
BASE COUNT          670 a         1169 c         1153 g          768 t
ORIGIN      
        1 atctggagct cggcgcgcaa ttgagcgacc acgttcagcg tcggcagttg cgaacaggcg
       61 acgaacaggc tcttgctccg cgccgtcacc gttcgtcgcg ccagttcaag aacttgatct
      121 tcggtgatct gaccaagctc tgcggtggtt ttgcaaaaga agctgttcaa tgtctcgacg
      181 tggatgccgg acgattcgag gtaatcgcgc aggccgtcat tgaccggctt taggtagggc
      241 gtcaccaccg ctgtgtttga aacgccggcg ctgcgcaatg cctccaccat cgcgccggcc
      301 gtgctgacca cggtggcgca cgtacgctcg cgcaacctgt ccaccatgcg ggcattgccg
      361 ctgggaccgc cgaggaatcc cgccgccgtg cagccataga tcacaagatc gaattgctcc
      421 gccgcaaatg gttcaatggc gtccaaggtc gcttcggcat aggtgggcaa gtcttctagc
      481 agcagcgtgc gtgccgggcg cttgacgcgc gccaccggta tcggcgccag cgcgggacac
      541 agcgcggaaa tttccggctc catcgtggtg ttgttctccg gcaccacgat cagtgtcttg
      601 atggtttgcc gcatgaaccc cccgccttct gcgtgttcat accgtcagct tgaacagcgt
      661 cgtggtttct atgtcgtcgc cataccagac gcggatggcg gcttcgcgct tgtcgatata
      721 ttccggcttg ccatcttcca gcatcatcgg cacatcgtgg cccggcacca gaatattgcc
      781 cggccgcttc cgccataatt tccagatcgc ctcaatcgac gccttgctga ccgcggcgtc
      841 gtaagtcatg tcggtcgtgc ccgagacaat ctcggcgcgg ttcttggcgg cgtcgccggc
      901 gaagatcacg tcgcgctcac cagcgtgtaa gacaaacacc aggcagccgg gcgtgtggcc
      961 gggtgcgaga tgcgcggtga taccgggaaa cacttcctcg ccatcggcgg ccttgtgcac
     1021 cgtcggccaa gtgttgagct cacgcacata aagctcgggc accggcccaa tgccccacgg
     1081 cgccttcaat gaccattcaa gctcgttcgc gccgatcacg atgcgggcct tgggaaacat
     1141 cgtccagttc acggaatgat cgtggtgcga gtgcgtgagc aaaagatcgg tgatatcggc
     1201 cggcttcaat ccacgctcgg cgaagcgctt gatcagcata gtgcgcatgc tgaagccgcc
     1261 ggtatcgatc agcacgatgc ggtcatgtgc gcgcagcaag accaccgagc tccagcccag
     1321 cccgccatgg cacaccgatt tacccggaaa gccctgaacg acgatgtcga gatcgtagtc
     1381 gttgatcttc attgctagac cagcggcgcg ttcgagaccg gcagcgccgg cagtccggcc
     1441 gtggcggcct ccttctgcaa ggcggcgatt tccttcgcgt gatacggcag gccggtgcgg
     1501 cggcgcgacg cttcgagccg gcgttcggtc tcgcccggca ttatgacttc atcgaagctt
     1561 tccgcggtcg gcacggcgtg gacgcgctgc accagcgtat ccatgcgctc gagatactcg
     1621 tcctttgaga tgaaaaggtc aggcttcatc gccatgaaaa agtgtccgac attctgcggc
     1681 tggtcgtaat cgaggaactg gttgcgcacg tcgccgccgt aggccgcgcc cgagatgacg
     1741 ccgctcatga catccatcag catcgaaatc cccgaaccct tcggcccgcc gatcggcaac
     1801 acgacgccgc cgtccagtgc cgcattcgga tcggtcgtcg gctggccgtt tttattgagc
     1861 gcgtagccga tcggaatttt ctggccgcgt cgcgccgcgc gcctgatttt gccgcgtgcg
     1921 gccacggccg gcgacatatc gagatcgaac ggcacttcct tgccggcggg tgcggcggcg
     1981 gcaaacggac tagtgccgag gaaagcgtcg cgtccgcccc agggcggcat ggcgcgcgag
     2041 gcgttggtga aaacgaacga gatcaatccg gcctcgagcg cctgtagcgt atagcaggcc
     2101 gccatgccga aatgcgtgct gcgccgcgcc gagacgatac cgacgccgaa ctccttcgcc
     2161 atctcgatgg ccgcggccat ggccttggtc gcgaccacga agccgtagcc attctggccg
     2221 tcaagcgagc cgatcatcgg cgtgacgcac tcgaccacca gattgggctt ctggttgacc
     2281 aatccgcgcc gcacccggtc gagatattgc ggcaggaatt gcagtccgtg ggtatcgacg
     2341 ccgcgcaagt cggcgcgcac cagacaaccg gccataatgg ctgcgtcttg ttcagacagg
     2401 ccatgcgcga ccagtaggcg gcggccgaag gcttccgccg cctcgaccgc ggcatagaac
     2461 ggcccctgca tcgtttcggg tttggtagct tcgctgactg tgctcatcgc gttcctgctc
     2521 aactgccatt cagaccatgc gcctcgggga agaacatgtc cttccacgag gccggcttct
     2581 tcttgaggcg gccaactttg tacatgaagt cgacgaactt catcacattg accggtgtgg
     2641 tcgtgtattc gacatccgga tcggccacca ttttttccag tgcatcgagc gggttcttgt
     2701 ccttggtcgc cgtcagatag gtttgcgcgg cctcgcgcgg atgtgccttg atgaaggcgt
     2761 tggcgtcgtt gaacgccgca acgaccgccg cgcatatttt cggattggca tcatggaatt
     2821 ttttcgtcgc caccagtgta ccattggtgt gcttgccgcc gacgacatca taggacttga
     2881 gcacctgatg gacgcccggc gtcgccagtt cgtaataata gaacggagaa ctggcgaaat
     2941 gtgaattgat ctcggaccca ccgcccatca ccgccgcggc ggcatccgca tgcgaacggg
     3001 tgatggtgat cgagtcgagc ttgtcgtaat gatcggcccc ccagatcttg gcggcttcca
     3061 tttccagtat cagtgcgtgg ccggtgagct tgactgcggg cagcgcgatc ttgtcattgt
     3121 cggtgaagtc tgcgatcgtt tttacatccg gattgcgcgt caccagcaca aacggcatcg
     3181 attgcatggc aatgagggcg cgcacttcct gcggcgtgcc gacggtcttt tcccaaatgg
     3241 tcgcaagcgt ggtagcgccc ggcccgatga agtcggcctg gccggccagt agcgcatcaa
     3301 gcacggccgc cgggccgacg cgttgcgtcc atgaggtttt gacatcgccg agaccagcgg
     3361 ccttggcgtt tttctcgatc aaatgttgcg cctcgatgac gatcatcggc aggtaaggca
     3421 ggccgaactg cttcgacatt cgtacttcgt tgacttcagc gtgggcggcg acgccgagtg
     3481 cggcggcgca taggctggcg gccccgagct ttctccacat aggttcctcc gtgccttctt
     3541 atgttcgtta gaattgataa agccctgccg gattgtcgac gagaatcttt tgcaactcgg
     3601 tgcgggtctt ggtccatccg gcgaggcggt tgagcgccgc accgtcgtct tccggtcgga
     3661 acggctcgat caccgcggga tcgcgcttgg cagcaccggg atgcggccaa tcggtgcccc
     3721 agacgatgcg atcggggttg gcggcgatca tcgcgcgagc
//
LOCUS       DBNGZE010000495         3595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_69318, whole genome shotgun sequence.
ACCESSION   DBNGZE010000495 DBNGZE010000000
VERSION     DBNGZE010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_69318"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC16FV_12720"
     CDS             complement(<1..650)
                     /locus_tag="AC16FV_12720"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR2850948.1"
                     /translation="MNAPDPKPETAMTLEVHVGFASEVGKRPANDDYVAACTGDAGRH
                     RRQDTLAVIADGMGGGPGGRLAAETSVRGFIDAYYSLPETLGVDRLAARSLASVNRWI
                     HAQGRQDPELKSMATTFSALILRGRSAHVAHVGDTRIYRLREHVLQRLTRDHVHAHPD
                     MRHVLYRAVGLEASVRADYAVHDVKAHDRFLLCSDGVHGVLSDERLRAILDARASP"
     gene            complement(663..1943)
                     /locus_tag="AC16FV_12725"
     CDS             complement(663..1943)
                     /locus_tag="AC16FV_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2850949.1"
                     /translation="MNWREFRQAGHWPTLLSAFLYFDVSFMAWVVLGPLALYLSQELG
                     LSIEEKFGIVAIPILAGALLRIPLGMIADHVGPKRTGQFAQLVVIVGLAYAWLSGLHS
                     KLEVEIMGLILGVAGASFAVALPQASRWYPPKYQGVVMGIAGAGNMGVVLDSMLVPWL
                     AETFGWQAVFGFLLAPLVIVFVFYSILAKDAPDRRAPVTFANYRTVLQDADTWWFMFF
                     YSITFGGFVGLGSALPLYFTSWYHVSGVAAGMMVAAVVFAGSMFRPVGGWLADRFGGL
                     RTLQILFAVVAVCYASIAYLPAGPAPAIESVSAAKVGGWTLSELPPAAWGAVAIFFVG
                     AMALGMGNGSVFQLVPLRFRNEIGVMTGLVGAAGGVGGFFLAKTLGWSKGMTGGFAAG
                     FWLFAALAAVGLFGLLMVKTRWRTTWGAASGARV"
     gene            complement(2270..3202)
                     /gene="secF"
                     /locus_tag="AC16FV_12730"
     CDS             complement(2270..3202)
                     /gene="secF"
                     /locus_tag="AC16FV_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR2850950.1"
                     /translation="MEFFKIKKDIPFMRHALAFNVISAVTFLLAVFFLITKGLNFGVD
                     FTGGTVMEISYSQPAEVPKIRDSLAKLGFQDVAVQNFGTARDVLIRLPLKEGVSSAKL
                     SETVMQGLRQAEPAVELRRVEFVGPQVGRELVENGALALLFVSIGIVLYLWFRFEWKF
                     GVATIIANLHDVVIILGFFAFFQWDFSLTVLAAVLAIFGYSVNESVVVFDRVRENFRK
                     LRKHTVPQIIDNAITRTISRTIITHGSTQMAVLAMLFFGGETLHYFALALTIGILFGI
                     YSSALVASPIVMWLGVSREDLMPVKKETLEDGRP"
     gene            complement(3212..>3595)
                     /locus_tag="AC16FV_12735"
     CDS             complement(3212..>3595)
                     /locus_tag="AC16FV_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR2850951.1"
                     /translation="TLPGMAGIALTVGMAIDAYVLINERIREELRNGNTPQASIYAGY
                     ERAFGTILDSNVTNLIAGLALFMFGTGPIKGFAVVLCLGILTSMFSSVMGSRALVNLI
                     YGGRRKLEKIPIGNTAWKETAPARG"
BASE COUNT          751 a         1146 c         1092 g          606 t
ORIGIN      
        1 tgcggcgagg cgcgcgcgtc cagaatggcg cgcaggcgct cgtcagacag cacgccgtgc
       61 accccgtcgc tgcacagcag gaaccggtcg tgcgctttca catcatggac ggcgtagtcc
      121 gcgcgcacgc tggcctcgag acccacggcg cggtaaagca cgtgccgcat gtcgggatgg
      181 gcatggacgt gatcacgcgt caggcgctgc aggacatgct cgcgcaggcg atagatgcgc
      241 gtgtcgccca cgtgcgccac gtgcgcgctt ctccctctca ggatgagcgc gctgaaggtc
      301 gtcgccatgc ttttcagctc cgggtcctgg cgcccctgcg cgtgaatcca gcgattgacg
      361 gaagccagcg accgcgccgc gaggcgatcc acgccaagtg tttccggcag gctgtagtag
      421 gcgtcgatga atccgcgcac gctcgtttcc gccgccaggc gtccgcccgg tccgccgccc
      481 atgccgtcgg cgatgacggc cagcgtatcc tgccgtcgat ggcgcccagc gtcgccggtg
      541 caggccgcca cgtaatcgtc gttcgccggc cgtttgccga cttcgctggc aaaaccgacg
      601 tgtacttcca gcgtcatggc ggtttccggt ttcggatcgg gcgcgttcat gatactgata
      661 atttatacgc gcgcgcccga ggccgcaccc caggtcgtgc gccaacgtgt tttcaccatc
      721 agcagaccga acaggccgac ggccgccagc gccgcgaaca gccagaaacc cgccgcaaat
      781 ccgccggtca tgcccttgga ccagccgagc gtcttggcga ggaaaaagcc gcccacgccg
      841 cccgcggcgc ctacgagtcc ggtcatgacg ccgatttcat tgcggaaacg caacggcacc
      901 agctgaaaca ccgagccgtt gcccatgccc agcgccatcg cgccgacgaa gaatatggcc
      961 actgcgcccc aggcagccgg cggcagttct gataatgtcc agccgccgac cttggcggcg
     1021 ctgacgctct ctatcgccgg cgccggaccc gcgggcagat aagcgatgct cgcgtaacac
     1081 acggccacca cggcaaacag aatctggagg gttcttagac cgccgaaacg atcggcaagc
     1141 cagccgccga cggggcggaa catcgaaccg gcgaacacca ccgcggccac catcatgccg
     1201 gcggcgacgc cggagacgtg ataccagctg gtgaagtaga gcggcaacgc gctgcccaat
     1261 cccacgaagc cgccgaaggt gatggagtag aaaaacatga accaccaggt atccgcatcc
     1321 tgcaacaccg tccggtaatt cgcgaacgtc accggcgcgc gccggtccgg cgcgtccttg
     1381 gccaggatgc tgtaaaacac gaacacgatc accagcggcg ccagcaggaa accgaacacc
     1441 gcctgccagc cgaaggtttc ggccagccac ggcacgagca tcgaatcgag cacgacgccc
     1501 atgttgccgg cgccggcgat gcccatcacc acgccctgat atttcggcgg ataccagcgc
     1561 gaggcctgcg gcagcgccac ggcgaagctg gcgcctgcca cacccaggat cagccccatg
     1621 atttctacct ccagcttgga gtgcagcccg gaaagccagg cataggcgag gcccacgatc
     1681 accaccagct gcgcgaactg gccggtgcgt ttgggaccga cgtggtccgc gatcatgccc
     1741 agcgggatgc gcagcagcgc gcccgccagg atcggaatgg cgacgatgcc gaatttttcc
     1801 tcgatggata atcccagctc ctggctgaga taaagcgcga gcggtcccag caccacccac
     1861 gccatgaaac tgacatcaaa atacaaaaac gccgacaaca gcgtcggcca gtggcccgcc
     1921 tgccggaact cgcgccaatt catgattcgc ctcctgtggc cgacggtgcg gcaccattgc
     1981 ccaccgccgt tcgggttttt ctaaggcaat cggcgtgcca tgacggcaaa gcaagctaac
     2041 tgcttgattc agcgggaaac aaacgtatta cgacgattca ggtgaatgcg cagatgcaca
     2101 gcgatggtga atacgaggga gaaatgcccg cgtttggtgc ggcgcaggta ggaaaaatac
     2161 ctgaaatatg attttgaacc gccaaggcgc caaggacgcc aagtttctcc tcaaaaaaca
     2221 gttttctggc ggcttggcat cttggcggta gatttgcttt ttacaagttt tagggacggc
     2281 cgtcttcgag cgtttctttc ttgaccggca tcaggtcttc acgcgagacg ccgagccaca
     2341 taacgatggg gctcgccacc agcgccgagg aataaatgcc gaaaaggatg ccaatggtga
     2401 gcgcgagggc gaaataatgc agtgtctcgc cgccgaagaa caacatcgcc agcaccgcca
     2461 tctgggtgct gccgtgggtg ataatggtgc gagaaatcgt gcgcgtgatg gcgttgtcga
     2521 tgatttgcgg caccgtgtgc ttgcgcagct tgcggaaatt ttcccgcacg cggtcgaaca
     2581 ccaccacgga ttcgttcacc gagtagccga agatggcgag taccgccgcc agcaccgtga
     2641 gcgagaaatc ccactgaaaa aaggcgaaga aaccgaggat gatcacgacg tcgtgcaggt
     2701 tcgcgatgat cgtcgccacg ccgaacttcc attcaaagcg gaaccacagg tagagcacga
     2761 tgccgatgga cacgaacagc agcgccagcg cgccgttttc cactaattcc ctacccacct
     2821 ggggtccgac gaattccacg cgccgcaatt cgaccgcggg ctcggcctgc cgcaggcctt
     2881 gcatgaccgt ttcggacagt ttggcgctgg acacgccttc cttcagcggc agtcggatca
     2941 gcacgtcgcg cgcggtgccg aaattctgca ctgccacgtc ctggaaaccc agcttggcga
     3001 gcgagtcgcg gattttcggg acctcggctg gctggctgta gctgatttcc atcaccgtgc
     3061 caccggtgaa gtcgacgccg aaattcaggc ctttcgtgat caggaaaaac accgcgagga
     3121 gaaacgtgac ggccgaaatc acgttgaacg ccagcgcgtg gcgcatgaag ggaatgtctt
     3181 tcttgatctt gaagaattcc atgatgcgtt cctatccccg cgccggagcg gtctctttcc
     3241 aggcggtatt gccgatgggt attttttcca gcttgcgccg accgccgtat atcaggttga
     3301 ccagggcgcg cgaccccatc accgaactga acatcgaggt caggatgccg aggcacaaca
     3361 cgacggcaaa gcccttgatg gggccggtgc cgaacatgaa gagcgccagt cccgcgatca
     3421 ggttggtcac gttggaatcg aggatggtgc cgaaggcgcg ctcgtagccg gcgtagatgc
     3481 tggcttgcgg cgtgttgccg ttgcgcaatt cctcgcgaat gcgctcgttg atgagcacgt
     3541 aggcgtcaat cgccatgccg acggtgagcg cgataccggc catgccgggt agcgt
//
LOCUS       DBNGZE010000496         5055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_692771, whole genome shotgun sequence.
ACCESSION   DBNGZE010000496 DBNGZE010000000
VERSION     DBNGZE010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_692771"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="AC16FV_12740"
     CDS             complement(<1..991)
                     /locus_tag="AC16FV_12740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR2850952.1"
                     /translation="MSRVFIVDGVRTPFLKARTEPGPFSASDLAVSCARPLLARQPFP
                     ASQLDEVIVGCVIPAADEANIARLIALRLGCGKRVPAHTVQRNCASGLQAIASAAERI
                     QLGRAHLVLAGGAEAMSRAPIQWNTAMSGWLGKMLRARGPLGRLQALARFRPSLLKPV
                     YSLLLGLTDPLVKLSMGQTAEVLAHRFGISRESQDVYALNSHKRLAAAFDAGHMNAEV
                     ETLYDVNERFYTEDTGLRRDTDLDQLAQLKPVFDRKYGLVTSGNSSQVTDGAAMLVIA
                     SEAAVKQHGLSVLGEWVDHEWAGVDPSQMGLGPVHAVAQLLARQKMKMRDLQQL"
     gene            complement(1026..3446)
                     /locus_tag="AC16FV_12745"
     CDS             complement(1026..3446)
                     /locus_tag="AC16FV_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR2850953.1"
                     /translation="MNTIFWILIAVVAVWGLAAQRVPGLVWATLLAAAIALWDILTQP
                     PTSLWIAVWSLFLVIVLPLAFTPLRRALISAPLLRVFKRIMPAMSDTEREALEAGTVW
                     WEAELFGGDPDWEQLFSLPQPSLSEEEQAFLDGPVEQLCTMLDDWDITHERRDLPPKV
                     WRFMKDKGFFGMIIPKQYGGLEFSAQAHSAVVTKVASRSITAAVTVMVPNSLGPAELL
                     LHYGTDKQKKHYLPRLARGKEVPCFALTGPEAGSDAGSMPDRGVVCRDTFKGKKNVLG
                     IRLNWEKRYITLGPVATVLGLAFRLFDPEHLLGEQEDLGITLALIPTDTPGVKIGTRH
                     NPLDIPFQNGPNWGENVFIPLDWIIGGRARVGQGWRMLMDSLAAGRSISLPALSCGAG
                     KVACRATGAYARIRRQFKLPIGKFEAVEEWLGRMAGYTYMMDAARTLTARAVDLGEKP
                     SVASAIVKYQLTEHMRRVVNDAMDIQGGSGICLGPRNFMGRAYQALPISITVEGANLL
                     TRALIIYGQGAVRCHPYVLNEMRAVAEPNQKRAVKDFDAAIFGHVRYTISNVARAFWL
                     GLTSARLVRSPVDGPAQRYVQKLTRLSAAFALISDLAMLTLGGALKRKEKISGRFADA
                     LSFLYLASAVIKRFEDEGRPVEDIPLMRYACRYCLHEAEKTLVVILKNFPNPFLAWLL
                     GGLVFPAGRRNHVPDDRMNHDCAALLLEPSAARDRLTRGIYLSPTTRDALGRLEDALP
                     KAIAAELLERRLAKLVEEQPLLHGDFEAQLKAALEQNVLSNAEAKLVRTAYAVRRGVI
                     AVDDFEKI"
     gene            complement(3553..3723)
                     /locus_tag="AC16FV_12750"
     CDS             complement(3553..3723)
                     /locus_tag="AC16FV_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR2850954.1"
                     /translation="MGMKSYMCVICGFVYEEERGRPEDGIPPGTRWEDVPENWKCPDC
                     GAAKSDFEIIQI"
     gene            3814..4788
                     /gene="hemH"
                     /locus_tag="AC16FV_12755"
     CDS             3814..4788
                     /gene="hemH"
                     /locus_tag="AC16FV_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR2850955.1"
                     /translation="MSDRVAVVLCNLGGPDSLEAVRPFLFNLFSDPDIFRLPLGWLTQ
                     RPFATLIAWRRTHEAAKGYAAIGGKSPILDYTRAQAGALQQALARSGQFDVLPCMRYW
                     HPLTDDVVAMLKQKNYARVILLPLYPQYSITTTGSAYNEFRRQCQRQHYQPSVTLVRQ
                     WYDQPDYQAAIVETLRAELKKFPDPDPARIELLFSAHGLPQKVVDGGDPYERQIRATF
                     DNVRAQLGWPHTTLCYQSRVGPLEWLRPYTEDVIREKAATGTKQMLVYPIAFVSDHVE
                     TLYELGITYAELARKHGMTHYRVVPALNSHPRFIAALKSLVLNAVSAP"
     gene            4785..>5055
                     /locus_tag="AC16FV_12760"
     CDS             4785..>5055
                     /locus_tag="AC16FV_12760"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR2850956.1"
                     /translation="MSDTTPPVVLVFAGSDPTGGAGITADVLTLASLGCHPAVVVTAV
                     TAQDTTGIKQFTCTETELVIAQARAVLEDMPVAAFKIGMLGNSAVA"
BASE COUNT          908 a         1647 c         1595 g          905 t
ORIGIN      
        1 ccagttgttg caggtcgcgc attttcattt tctggcgcgc cagcaactgg gcgacggcat
       61 gcaccggccc gagacccatc tggctcgggt cgacaccggc ccattcgtgg tccacccatt
      121 cgcccagcac ggacagcccg tgctgcttca ccgccgcttc gctggcgatc accagcatgg
      181 cggcgccgtc ggtgacctga gagctgttgc cggaggtcac cagaccgtac ttgcggtcga
      241 acaccggttt aagctgcgcc agctgatcga gatcggtgtc gcgccgcaga ccggtgtctt
      301 cggtgtagaa acgttcgttt acgtcataga gcgtctcgac ctcggcgttc atgtgtccgg
      361 catcgaaggc cgccgccagg cgcttgtggc tgttgagggc atagacatcc tggctctcgc
      421 gcgagatgcc gaagcggtgt gccagcacct ccgcggtctg ccccatggac agtttcacca
      481 gtggatcggt aagaccgagc agcagggaat acaccggctt gagcagcgat ggccggaaac
      541 gcgccagcgc ctgaagccgg ccaagcggac cgcgcgcgcg cagcatcttg ccgagccagc
      601 cgctcatggc ggtgttccac tggatgggcg cgcggctcat ggcctcggcg ccgcccgcga
      661 gcaccagatg cgcccggccg agctggatgc gctcggccgc gctcgcgatc gcctgcaaac
      721 ccgaggcgca gttgcgttgc actgtgtgcg ccggcacgcg cttgccacaa ccaagacgta
      781 acgcgatcag gcgcgcgata ttggcctcgt ccgccgccgg gatgacgcag cccacgatga
      841 cctcgtcgag ttgcgacgcc ggaaacggct ggcgcgccag cagcgggcgg gcgcaagaca
      901 cggcgagatc ggaggccgaa aacggccccg gctcggtacg tgccttcaga aacggcgtgc
      961 gtaccccatc gacgataaag actcggctca tgtaactacc ttctaaatcc tgttaaccct
     1021 gtcaattata ttttctcgaa atcatccacc gcgatcacgc cacgccgcac ggcataggca
     1081 gtgcgcacca gcttggcctc ggcgttgctc aatacattct gctccagcgc cgccttcagc
     1141 tgcgcttcga aatcgccatg cagcagcggc tgctcttcca ccagtttggc tagccggcgt
     1201 tccagcagtt ccgcagcgat ggccttgggc aaggcgtcct cgaggcgacc gagcgcgtcg
     1261 cgcgtcgtcg gcgacagata aatgccgcgc gtcaaccggt cacgcgcggc cgagggctca
     1321 agcaacagcg ccgcgcagtc atgattcatc ctgtcatccg gcacatgatt gcgccggccg
     1381 gccgggaaca ccagcccgcc cagcagccag gccaggaagg ggttggggaa attcttgagg
     1441 attaccacca gcgtcttctc ggcctcatgc aggcagtacc ggcaggcgta gcgcatcagt
     1501 ggaatatcct ccaccgggcg gccctcgtcc tcgaagcgct tgatcacggc cgaggccagg
     1561 tacagaaagc tcagcgcgtc ggcgaagcgg ccggagatct tttccttgcg cttgagcgcg
     1621 ccgccgagcg tcagcatggc gaggtcggag attagtgcaa acgccgctga caggcgcgtg
     1681 agtttctgca cgtagcgctg cgccggtccg tccacgggcg agcgcacgag acgcgcggag
     1741 gtcaagccca gccaaaaggc gcgcgccacg ttgctgatgg tgtagcgcac gtggccgaag
     1801 atggccgcgt cgaaatcctt cacggcgcgt ttttgattcg gctccgccac cgcgcgcatt
     1861 tcattcagca cataaggatg gcaacgcacc gcgccctggc cgtaaatgat gagcgcgcgc
     1921 gtgagcaggt tggcgccctc gaccgtgata ctgatgggca gtgcctggta cgcgcgcccc
     1981 atgaaattgc gcgggccgag gcagatgccg gagccgccct ggatgtccat ggcgtcgttc
     2041 accacgcggc gcatgtgttc ggtgagctgg tatttgacga tggccgaggc caccgacggt
     2101 ttctcgccca ggtccacggc gcgcgcggtg agcgtgcgcg ccgcgtccat catgtaggtg
     2161 tagccggcca tgcgcccgag ccattcttcc accgcctcga atttgccgat gggcagtttg
     2221 aactggcggc ggatgcgcgc ataggcgccg gttgcgcggc aggcgacctt gcctgcgccg
     2281 caggacaacg ccggcagcga gatcgaacgc ccggcggcga gcgagtccat cagcatgcgc
     2341 caaccctggc cgacgcgcgc gcgtccgccg atgatccagt cgagcgggat gaacacattc
     2401 tcgccccagt tgggcccgtt ctggaacgga atgtcgagcg ggttgtggcg cgtgccgatt
     2461 ttgacaccgg gggtatcggt ggggatgagc gcgagcgtga tgccgaggtc ttcttgctcg
     2521 cccagcagat gctccggatc gaacagacgg aacgcgagcc cgagcaccgt ggccaccggc
     2581 ccgagcgtga tgtagcgctt ctcccagttg aggcggatgc cgagtacgtt cttctttccc
     2641 ttgaacgtgt cgcggcagac cacgccgcga tcgggcatgc tgccggcgtc cgacccggcc
     2701 tcggggccgg tgagggcgaa gcacggtact tccttgccgc gcgcgagacg cgggagataa
     2761 tgttttttct gtttatcggt gccgtagtgc agcagcagtt cggccggacc gagcgagttc
     2821 ggcaccatca ctgtcaccgc cgcggtgatg gagcggctcg cgaccttggt caccaccgcc
     2881 gaatgcgcct gcgcggagaa ttccaggccc ccgtattgct tcgggatgat catcccgaag
     2941 aagcccttgt ccttcatgaa gcgccacacc ttcggcggca ggtcacggcg ctcgtgcgtg
     3001 atgtcccagt cgtcgagcat ggtgcacagt tgttctaccg gtccgtcgag aaaggcctgt
     3061 tcttcctcgg acagtgacgg ctgcggcaga ctgaatagct gctcccagtc gggatcgccg
     3121 ccgaacagtt ccgcctccca ccacacggtg ccggcctcga gcgcctcgcg ctcggtgtcc
     3181 gacatggccg gcatgatgcg cttgaacacg cgcagcagcg gagcgctgat caaggcgcga
     3241 cgcagcggcg taaacgcgag cggcaagacg atgacgagaa acagcgacca gacggcgatc
     3301 cacagcgaag tcgggggctg ggtcaggatg tcccacaggg cgatggcggc ggcgagcagc
     3361 gtggcccaca cgaggcccgg cacgcgctgg gcggccagtc cccagaccgc caccaccgca
     3421 atgagaatcc agaaaatggt gttcatggtc cctgccttgg gtgccctttt ccttgttatt
     3481 cggggtagtt aaagatgccc tcccattgta gtccacggac cggcccggct ccgggaacgg
     3541 ggtgaaaccg ggtcaaatct ggataatttc aaaatcggac ttggccgcgc cgcaatcggg
     3601 gcatttccag ttttccggca catcctccca gcgggttccc gggggaatgc cgtcttccgg
     3661 ccggcccctc tcttcctcgt aaacgaaacc gcagatcacg cacatgtacg acttcattcc
     3721 caccccttgc tgccggttaa tgcaatgtaa agcggaataa tcgcattttc atcttcgtgg
     3781 tgaaaccacg gggccggcgg taaaattccc gcgatgtcag accgtgtcgc ggtggtgctg
     3841 tgcaacctgg gcgggccgga ttcgctggaa gcggtgcgcc ccttcctgtt taaccttttt
     3901 tccgatccgg atatttttcg cctcccgctc ggctggctta cccaacgccc gtttgcgacc
     3961 ctgatcgcgt ggcggcgcac gcacgaagcc gccaagggtt acgccgccat cggcggcaaa
     4021 tcccccatcc tcgattacac acgcgcacag gccggcgcct tgcaacaggc actcgcgcgc
     4081 tccggtcaat tcgacgtgct accgtgcatg cgttactggc atccgctcac cgacgatgtg
     4141 gtggcgatgc tcaaacagaa aaattacgcg cgcgtgatac tgctgccgct gtatccgcag
     4201 tattccatca ccaccaccgg tagtgcctat aacgagttcc ggcgccagtg ccagcggcaa
     4261 cactatcagc catcggtcac gctggtgcga caatggtacg accagcccga ttaccaggcc
     4321 gcgatcgtgg agacgctgcg cgccgagctg aaaaaatttc ccgatcccga tccggcgcgc
     4381 atcgaattat tattctcggc tcacgggttg ccgcagaagg tcgtggatgg cggcgatccc
     4441 tacgagcgcc agatccgcgc gaccttcgat aacgtgcgtg ctcagctggg ctggccgcac
     4501 accacgctgt gctatcaaag ccgggtcggg ccgctcgaat ggctgcggcc ctataccgag
     4561 gacgtgattc gcgagaaggc cgcgaccgga acgaaacaga tgctggtgta tccgattgcg
     4621 ttcgtgtcgg accatgtcga gacgttgtat gaactcggta tcacttacgc cgaactggca
     4681 cgcaagcatg gcatgacgca ctatcgtgtc gtgccggcgc ttaacagcca tccgcgtttt
     4741 atcgcggcgc tgaaatcgct ggtgctcaat gcggtgtcag cgccatgagc gacaccaccc
     4801 cgcctgtggt tctggtattc gccggcagcg accccaccgg cggcgccggc atcactgccg
     4861 acgtgctgac gctggcgagc ctcggctgcc acccggccgt ggttgtcacc gccgtgaccg
     4921 cgcaggacac caccggcatc aagcagttca cctgcaccga gaccgagctc gtgatcgcgc
     4981 aggcgcgcgc ggtgctggaa gacatgccgg tggcggcctt caagatcggc atgctcggca
     5041 acagcgcggt ggcca
//
LOCUS       DBNGZE010000497         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1106764, whole genome shotgun sequence.
ACCESSION   DBNGZE010000497 DBNGZE010000000
VERSION     DBNGZE010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1106764"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16FV_12765"
     CDS             <1..633
                     /locus_tag="AC16FV_12765"
                     /inference="COORDINATES: protein motif:HMM:NF027400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR2850957.1"
                     /translation="KANTINGLNEGDIIMISDCANADIMQITQLNPTDPHIVHNSGDN
                     DPLPGNATQVLSKSYSPSTSPDGTRIMKFRTTRYFIGTGASGNPALFRQDNIAAAVEL
                     VDGVENLQVLYGKDTDSDGIPNVYLKAGDAGLQSAADWSSVRSVRIGILARTVNDKDT
                     DVDSGSYDIDGDCPTGVPVTDACYEFTVQAPGDHNKRRLFQAAVQLRNLQ"
     gene            630..1124
                     /locus_tag="AC16FV_12770"
     CDS             630..1124
                     /locus_tag="AC16FV_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR2850958.1"
                     /translation="MNGLTPMFQKKSPARTGCRDSYQSTGGRQQRGTALVMSMVILLI
                     LTILGIAAMGTASLEEKMSGNTQEGTRAFEAAESGLTEALNTPGTLMLSGTPTPLNFN
                     YNTMNAQANVSTTFIQYSSPKRGSGYSNKNFQSANFDQSSTGTTGVGAKAVVHQGIAQ
                     IVPQ"
     gene            1187..>2993
                     /locus_tag="AC16FV_12775"
     CDS             1187..>2993
                     /locus_tag="AC16FV_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850959.1"
                     /translation="MAALITLSATGPVGAKDTDVYLMSPTVARDDSPNVMIILDNSGS
                     MSTVISNTRPAYDPSIDYCTGDLDTLTGISGANAGKPSNCSSISGRIFWSFSSSPPST
                     SSSNWFASSKNMCFDSTTSLNTSGSYGSTKIVGYVSGTGWRSLSGRTNSDFTSVDCQA
                     DGTTSGQAVGDNTFPRRSNSAYTSISSQEFNWSNFTSNDKPILYSSNYVNYWRNSALL
                     TSKTRLSVAKDAVKSIIDANKSVRYGLTVFNDDGPSPDGGRVLMRIDTMSDARRTTMK
                     NIVDSLTASTYTPLAETMWEAYRYFAGLSATYGNPSPLQSPHQDSCAQNAGSGYCANG
                     GLYRATNGEYSNSSGAILSGGTVDASYNDGVYVSPFKYGCQTAYIIYVTDGDPTNDSA
                     ANSNVASLIGASCDGSSCLDDLAGWMHDQDVYSGLPGKQTVKTYTIGFGGGISASGLA
                     LLQQTASKGGGTYYTADDADQLASSLQGAITDILQTTASFTAPSLSINAFNKLYNRDD
                     VYFALFKPSSSVAWDGNIKRYRLCNTSDVTAYNCAYGEVIDLNNTAAIDAQTSKIKTT
                     AVSYWGSIADGPEVTLGGAGAQISQDSKVPRALYTY"
BASE COUNT          699 a          922 c          795 g          577 t
ORIGIN      
        1 aaggccaata ccatcaacgg tctgaacgaa ggcgacatca tcatgatctc ggactgcgcc
       61 aacgccgata tcatgcaaat tacccagcta aatcccactg atccgcatat cgtgcataac
      121 agtggcgaca acgaccccct ccccggtaat gccacccagg tactgagcaa atcctatagt
      181 ccttccacgt ccccggatgg aacaagaatc atgaaattcc ggacgacccg atatttcatc
      241 ggcactggcg ccagtggaaa tccggcgttg ttccggcagg ataatatcgc ggcggcggtt
      301 gagctggtgg atggcgtcga aaatctccag gtcctatacg gcaaggatac cgacagcgac
      361 gggataccca acgtttatct caaagccggc gatgcagggt tacagtcagc tgccgactgg
      421 tcctcggtca gaagcgtgcg catcggcatt ctcgcgcgaa ccgtgaatga caaggatacc
      481 gatgtcgaca gtggatccta tgatatcgat ggcgactgtc ccacgggtgt gcctgttact
      541 gatgcctgtt acgagttcac tgtgcaggca ccgggcgacc ataacaaacg tcgcctgttc
      601 caagcggcag tgcagctgag aaatctgcaa tgaacggcct gacgcccatg tttcagaaaa
      661 aaagccccgc ccggaccggc tgcagggaca gctatcaatc caccgggggc cggcagcagc
      721 gcggcaccgc gcttgtcatg agcatggtga ttctgctgat cctgaccatc ctcgggattg
      781 ccgccatggg caccgcctcg ctggaagaga agatgtccgg caatacacag gaaggcacgc
      841 gcgccttcga ggcggcggaa tccgggctga cggaagccct caataccccc ggcactctca
      901 tgctcagcgg gactccgacc ccccttaact tcaattacaa caccatgaat gcccaggcga
      961 atgtcagcac gacattcatc cagtattcgt cacccaaacg cggctcggga tacagcaaca
     1021 agaatttcca gtcggccaat tttgaccagt cgagtaccgg cacaaccggt gtcggcgcca
     1081 aggctgtcgt tcatcagggc attgcccaaa tcgtacctca ataggcaggt gaatcatcat
     1141 gcgtaactat cagagcctac ttaatcgaat ctcatccctt ttaggcatgg cggcgttgat
     1201 cacgctttct gccacgggac ccgtcggcgc caaagacacc gacgtctatc tcatgtcgcc
     1261 gacggtggca cgggacgatt cgccgaacgt aatgatcatt ctggacaact cgggcagcat
     1321 gagcaccgtc atcagcaaca cgcgcccggc ctatgacccg tcgattgact attgcaccgg
     1381 ggatctggac accctcaccg gcatctccgg cgccaatgcc ggcaaaccca gcaattgctc
     1441 gtccatcagc ggacgcattt tctggtcgtt cagcagcagc ccgccatcca cctcctccag
     1501 caattggttc gcttcgagca agaatatgtg cttcgactcg acgacgtcgc tgaacacgag
     1561 cggttcatat ggttcgacga aaatcgtggg ctatgtcagc ggcacgggct ggagatcgct
     1621 cagcggccgc acaaactccg attttacctc cgtggattgt caggccgacg gcactaccag
     1681 cggccaggcc gtgggtgata ataccttccc gcgtcgctca aatagtgcct ataccagcat
     1741 ctcaagtcag gaattcaact ggagcaattt cacatccaac gacaagccca tcctttacag
     1801 ttcgaattac gtcaactact ggagaaactc agccctgctg acatcgaaaa cccgcctcag
     1861 cgtcgccaag gatgcggtca agagcatcat cgatgcaaac aagtcagtcc gttacgggtt
     1921 gaccgtgttc aacgatgacg gtccatcgcc ggatggcggg cgcgtgctga tgcggatcga
     1981 caccatgagc gatgcgcgcc gcactacgat gaaaaatatc gtggacagtc tgacggccag
     2041 cacctatacg ccgttggcgg aaaccatgtg ggaagcctat cgttactttg ccggtttgag
     2101 cgccacttac ggaaatccct cgcccctcca atcaccgcat caggattcct gcgcccagaa
     2161 cgcggggagc ggctattgcg ccaacggcgg cctttaccgg gcgaccaatg gcgaatacag
     2221 caacagcagc ggggccattc tgagcggtgg aacggttgac gcttcctaca acgacggggt
     2281 ctatgtttct ccgttcaagt atggctgcca gacggcttat atcatctatg tcacggacgg
     2341 cgaccctacc aatgacagtg ccgccaacag caatgtcgcg agcctgatcg gcgccagttg
     2401 cgacggcagc agctgcctgg acgacctcgc cggctggatg catgaccaag atgtttactc
     2461 cggcctgccg ggaaaacaga cggtcaagac ttataccatc ggttttggcg gcggcatttc
     2521 ggcatccggc cttgccctgt tgcaacaaac cgcctcgaaa ggcggcggca cctactacac
     2581 cgccgacgac gccgaccagc tggcgtcgtc gctgcaaggg gccattaccg acatcttgca
     2641 gaccacggcg tcgtttaccg cgcccagctt gtccatcaac gcgttcaaca aactgtataa
     2701 ccgggacgac gtctacttcg cgctgttcaa accctcctca tctgtggcct gggatggcaa
     2761 catcaagcgc tacaggcttt gcaatacctc ggatgtgacc gcttacaatt gcgcctatgg
     2821 ggaagtcatc gatcttaata acaccgctgc catcgatgcc cagaccagca agatcaaaac
     2881 cacggcggta agctactggg gctcaatcgc cgacggtccg gaagtcacgc tgggcggcgc
     2941 gggtgcgcag atcagccagg acagcaaggt gccgcgggca ctttatacct acc
//
LOCUS       DBNGZE010000498         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1803664, whole genome shotgun sequence.
ACCESSION   DBNGZE010000498 DBNGZE010000000
VERSION     DBNGZE010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1803664"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="AC16FV_12780"
     CDS             complement(<1..306)
                     /locus_tag="AC16FV_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850960.1"
                     /translation="MPASQKPESATDKLQRRNRELSILNTIAQALNREVDLARALQAA
                     LAHVAELLDLKTGWVWLLRDETGESYLAAAQNLPPGLAHYPQRMEGTCYCLDTYRAGD
                     "
     gene            408..1052
                     /locus_tag="AC16FV_12785"
     CDS             408..1052
                     /locus_tag="AC16FV_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850961.1"
                     /translation="MPLAHLRRISIRDDYFNPQAVLPYGLLRGEIRATLKDAHKFIHS
                     LNTMLYQQTGQRVEDILMAATFSGMLSEFIVRQLSKHSTALTRNLYHNGHPDLVPVNR
                     YPGNAVQYGHEGVEIKTSRYASGWQGHNPERIWIMVFRYDNDPYKPEKDPRSLEERQP
                     FQIVEVLAAELDKSDWSSQGRGSGSRRTPTASIIASGTVKLRANWIYRNPDFAT"
     gene            complement(1055..1945)
                     /locus_tag="AC16FV_12790"
     CDS             complement(1055..1945)
                     /locus_tag="AC16FV_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR2850962.1"
                     /translation="MTYEILHADCMEWLAACPPNSFHACITDPPYGLLEYQSEHLTKR
                     RNGRGGVWRIPPSFDGSNRQPLPRFTVLTEEDRKNLSEFFSEWARRLLPVLVPGAHVF
                     IASNPLVSHLVGASITEGGFEKRGEIIRLVRTFRGGDRPKGAEEEFREVSAMPRSAFE
                     PWGLFRRPFEGTLAENLRRWGTGGLRRPNSEAPFTDVIPSGRTPQEERAIAPHPSLKP
                     QKFLRQLVWAAMPLGKGKILDPFAGSGSTLAAAESLGYDSVGIEVDEEYYGLAAEAIP
                     LLARIPSQFNFLEPSSLDSE"
     gene            complement(1987..2178)
                     /locus_tag="AC16FV_12795"
     CDS             complement(1987..2178)
                     /locus_tag="AC16FV_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850963.1"
                     /translation="MGTYGLDGVLTAWKTGKLTTEQAVGQILQLLQELEERLAKVETV
                     INPPRPPTPHRRRRHAEHE"
BASE COUNT          477 a          672 c          670 g          523 t
ORIGIN      
        1 gtcgcccgcg cggtaagtgt cgaggcaata gcacgtgcct tccatgcgct gaggatagtg
       61 cgccagccct ggcggaaggt tctgcgcggc ggccagatac gattcgccgg tttcatcgcg
      121 caggagccaa acccagcctg tcttcaggtc gagcagctcg gccacgtgcg cgagcgcggc
      181 ctgcagtgcg cgcgcgaggt ccacctcgcg gttcagcgcc tgcgcgatgg tgttgaggat
      241 cgagagctcg cggttgcgcc gctggagctt atcggtggcg gattcgggct tctgggacgc
      301 aggcatcagg ccatccctcg ttgacttcgc cgtgcattgt agcgcggttg cgagatttgg
      361 ggcaaaagga ggtctgtgcc ataatcgcgc agagtctgag acaaaccatg ccactcgccc
      421 atctccgccg catttcaatt cgtgacgatt acttcaaccc gcaagccgtc ttgccctatg
      481 ggctactgcg tggcgagata agggcaacgc taaaagatgc ccacaagttt attcacagcc
      541 tgaataccat gctttaccag caaaccggtc agcgcgttga agacatttta atggcagcca
      601 ctttcagcgg aatgttgagc gaatttattg ttcgccagtt atccaagcac agcacagctc
      661 tgacgcgcaa tctgtatcac aatggccatc ctgatcttgt gccggtcaac cgctatcctg
      721 gcaacgctgt tcaatatggg cacgaggggg tagagatcaa gacttcccga tatgcaagtg
      781 gttggcaagg ccataacccg gaaagaatct ggatcatggt ctttcgatat gacaacgatc
      841 cttacaagcc ggaaaaagat ccgcgctctc tggaagagcg acaacctttt caaattgttg
      901 aagttttggc agcggagctc gacaagagcg attggagttc gcagggaaga gggtcaggca
      961 gccgccggac gccgacggcg tcaatcattg cttcgggcac tgttaaattg cgcgctaact
     1021 ggatttatcg aaatccggac ttcgcgacct gaactcactc agaatcgagg gatgacggtt
     1081 cgagaaaatt gaactgactt ggaatcctcg ccaatagtgg aatggcctct gccgctagcc
     1141 cataatattc ctcatccact tcaattccca cactgtcata ccctaacgac tctgctgccg
     1201 caagcgttga gccgctcccg gcaaacgggt caagaatctt gcctttgccg agtggcattg
     1261 cggcccatac caattggcgg aggaacttct gcggcttgag gctgggatga ggcgcaattg
     1321 cccgttcttc ttgcggcgtg cgcccgctgg gaatcacgtc ggtaaagggg gcctcggagt
     1381 tcggtcgccg caggccacct gtcccccatc ggcggaggtt ttcggccagt gttccttcaa
     1441 acggcctacg gaataatccc cacggctcga aggctgaacg cggcatcgcg ctgacttccc
     1501 ggaattcctc ttccgcacct ttgggtcggt ctccgcctcg gaatgttcgt accaagcgga
     1561 tgatctctcc gcgcttttca aaaccgccct ctgttatgct ggccccaacc aaatgggaga
     1621 ccagcggatt gctggcgata aacacgtgcg caccggggac aagaaccggc aggagtcgtc
     1681 tggcccactc tgaaaagaac tccgacaggt ttttccgatc ttcttcggtg agtacggtaa
     1741 agcggggcag gggctgtcgg ttgctgccgt cgaacgaggg tgggatacgc cacacgccgc
     1801 cacgcccatt tcggcgcttg gtcagatgct cagactgata ttcaagcagc ccgtacggcg
     1861 ggtcagtgat gcaggcatgg aatgaattgg gcgggcaggc cgccagccat tccatacagt
     1921 ctgcgtgaag gatctcgtaa gtcatcgctc caaccttaga ggacagccct ccccattata
     1981 tgcgaattat tcatgctccg cgtgcctccg ccgccggtgc ggggtcggag ggcgaggtgg
     2041 gttgatcacc gtctctactt tcgccagccg ctcttccagc tcttgcagca gttggaggat
     2101 ttgccccacc gcctgctcgg tggtgagttt gcccgttttc catgccgtca gcacgccgtc
     2161 cagcccgtag gtgcccatag cgcctccttg tacacgtttc cgtgtaagtc gtcgcaattt
     2221 tttcgggagc gatggctccc gaattatctg tcagcatttt acacggaaac gtgtatcctg
     2281 tcaatgggca atttcacgcc ttgccggtct tgtatgagaa gttggcgcgt tcggcgacta
     2341 tg
//
LOCUS       DBNGZE010000499         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1107840, whole genome shotgun sequence.
ACCESSION   DBNGZE010000499 DBNGZE010000000
VERSION     DBNGZE010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1107840"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16FV_12800"
     CDS             complement(<1..296)
                     /locus_tag="AC16FV_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR2850964.1"
                     /translation="METADHEVLRIACDWLADGETVYLVTVAKTFGSSPRPPGSLLAL
                     AASGRVAGSVSGGCIEYDLAARLRDHRMPEQLPGNVTYGVTREEAQRFGLPCGG"
     gene            complement(299..2458)
                     /locus_tag="AC16FV_12805"
     CDS             complement(299..2458)
                     /locus_tag="AC16FV_12805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR2850965.1"
                     /translation="MNTSVNLNRREFLKVSAAAAGGLLIGLYLPGRLEAAAGPHTEAE
                     FMPNAFLRIAPDNTVTVIVKHLEMGQGVYTGLPTIVADELDADWSQIRVESAPADVKR
                     YANLKWGGQAQGTGGSTSVNNSWEQMRRVGATARAMLVGAAAQKWNVKPGTLRTDNGY
                     VIDAKHKRRASYGELATLAATLTPPADVPLKNPKQWKLIGKHASRTDSADKSSGKAIY
                     ASDIRLPGMLTALVARPPLFGAKAKGFNAEAVKAMPGVKAVVAIPQGVAVVATDFWSA
                     RQARDALRVEWDDSAAEKRSSDELLAQYRELAQKPGAIAHRAGDVARGLNDATKKIEA
                     TFEFPYLAHAPMEPLACVVRLSDNACEVWAGSQLQTFDQATAAQIAGLKPEQVTIHTL
                     LAGGSFGRHANPTADYVSEGVEVAKATRALNAPIRLMWTREDDIHGGYYRPMYVHTIA
                     AGLDQRGRPVAWQHRIVGQSILTGTPFAGMIKDGIDVTSVEGASNLPYAIPNMLVDLH
                     TTQVGVPVLWWRSVGSTHTAFSTEVMINELAVAAKQDPVAFRRALLKNHPRHRGVLDL
                     AAQKAGWGKPQPKGRWRGVAVHESFNSYVAEVAEISLADGKPKVERVVCAVDCGVAVN
                     PDVVRAQMESGIIFGLSAALYGEIALKDGQVVQSNFHDYPILRINETPKIEVHIVPSH
                     AKPTGVGEPGVPPIAPAVANAMFAATGKPVRRLPIRV"
     gene            complement(2455..2925)
                     /locus_tag="AC16FV_12810"
     CDS             complement(2455..2925)
                     /locus_tag="AC16FV_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR2850966.1"
                     /translation="MINLLVNGKQQEVDVAADTPLLWVLRDHVGLTGTKYGCGIAACG
                     ACTVLIDGQPVRSCITPASSVSGKKITTIEGLSPEHAHPVQRAWIAEDVPQCGYCQSG
                     QILSAVALLSAKPKPTDQDIDEAMSGVLCRCGTYVRIRNAIKHAARLMAKGDVA"
     gene            3117..3320
                     /locus_tag="AC16FV_12815"
     CDS             3117..3320
                     /locus_tag="AC16FV_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HIR2850967.1"
                     /translation="MNERLTELEVRVAFQDKTIQDLNEVVTLQQRQIDRLTQELEAVK
                     SRLSALAPSMVIPQEDEKPPPHY"
     gene            3496..>3860
                     /locus_tag="AC16FV_12820"
     CDS             3496..>3860
                     /locus_tag="AC16FV_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2850968.1"
                     /translation="MSFSSLGLSAELLRAVSEKGYTQATPIQLQAIPVILEGRDILAG
                     AQTGTGKTAGFTLPLLQRLTAGANTSASPARHPVRALIVVPTRELAAQVQESVRTYGR
                     HLKVRSTVIFGGVGFNPQA"
BASE COUNT          655 a         1248 c         1221 g          736 t
ORIGIN      
        1 ttgccgccgc acggcaggcc aaagcgttgt gcctcctcac gcgtcacgcc gtaggtaacg
       61 tttcctggga gttgttccgg catgcggtgg tcacgcaggc gcgcggcgag atcgtactcg
      121 atgcagcctc ctgacaccga gccggcgacg cggccggagg ccgcgagcgc cagcagcgag
      181 cccggcggac gcggcgagga gccgaaggtc ttcgccactg tgacgagata caccgtctcg
      241 ccgtcggcga gccagtcgca ggcgatgcgc aagacttcgt gatcggcggt ttccatggct
      301 agacccgtat cggcagacgc cgtaccggct tgccggtggc ggcgaacatg gcgttggcca
      361 cggccggcgc aatcggcggc acgcccggct cgccgacgcc ggtcggcttg gcgtggctcg
      421 gcacgatgtg cacctcgatc tttggcgtct cgttgatgcg caggatcgga tagtcgtgaa
      481 agttggactg aaccacctgg ccatccttga gcgcgatctc gccgtacagc gccgccgaga
      541 gtccgaagat gatccccgat tccatttgcg cgcgcaccac gtccgggttg acggccacgc
      601 cgcagtcgac ggcgcacacc acgcgttcga ccttcggctt gccatcggcg agactgatct
      661 ccgccacctc ggcgacgtac gagttgaacg actcatgcac cgccacgccg cgccagcgtc
      721 ctttcggttg cggtttgccc cagccggcct tctgcgccgc gagatcgagc acgccgcgat
      781 ggcgcggatg gttcttgagc agtgcacgcc gaaacgccac cggatcttgc ttggccgcga
      841 cggcaagttc attgatcatg acttcggtcg agaacgcggt gtgcgtgctg cccacgctgc
      901 gccaccacaa caccggcacg ccgacttggg tggtgtgcag gtccaccagc atattcggta
      961 tcgcgtaggg gaggttggag gcgccttcga ccgaggtgac gtcgatgccg tccttgatca
     1021 tgcccgcgaa cggcgtgccg gtaaggatgg actgcccgac aatgcggtgt tgccaggcca
     1081 cgggccggcc gcgctggtca agaccggcgg cgatcgtgtg cacatacatc ggccggtagt
     1141 agccgccgtg gatatcgtcc tcgcgcgtcc acatcagtcg gatcggcgcg ttcagggcgc
     1201 gcgtggcctt ggcgacttca acaccctcac tgacataatc cgcggtcggg ttcgcgtgcc
     1261 gtccgaaact gccgccggcg agcagcgtgt ggatcgtgac ctgctcgggt ttcagtcccg
     1321 cgatttgcgc ggcggtggcc tggtcgaacg tctgtaactg cgagccggcc cagacctcgc
     1381 aggcgttgtc actcagtcgc acgacgcagg ccagcggctc catcggcgcg tgcgccaagt
     1441 agggaaattc gaacgtggcc tcgatttttt tcgtggcgtc gttcagtcct cgcgcgacgt
     1501 cacccgcgcg gtgcgcgatg gcaccgggtt tttgggccaa ttcccgatat tgcgccagca
     1561 gctcgtcgct cgaacgcttt tcggcggcgc tgtcgtccca ctccacgcgc aatgcgtcgc
     1621 gcgcctgcct ggccgaccag aaatcggtgg caaccaccgc cacaccctgg gggatcgcga
     1681 ccacagcctt cacgcccggc atcgccttca ccgcctcggc attgaagccc ttggccttgg
     1741 cgccgaacag cggcgggcgc gccaccagtg cggtgagcat gccgggtagg cggatgtcgc
     1801 tggcgtaaat cgccttaccg ctggatttat cggcgctgtc ggtgcgcgac gcgtgcttgc
     1861 cgatgagttt ccattgcttg gggttcttga gtggcacgtc cgccggtggt gtcagcgtcg
     1921 cggcgagcgt ggccagttcg ccgtagctcg cgcggcgctt gtgtttcgcg tcgatgacgt
     1981 aaccattgtc ggtgcgcagc gtgccgggtt tcacgttcca cttctgcgct gcggcgccga
     2041 ccagcatggc gcgcgccgtg gcaccgacgc ggcgcatctg ttcccaggaa ttgttcacgc
     2101 tggtgctgcc gccggtgccc tgcgcctggc caccccactt gagattggcg tagcgcttga
     2161 cgtcagccgg cgcggattcg acgcggatct ggctccagtc tgcgtccagt tcgtccgcca
     2221 cgatggtcgg caagccggta taaacgcctt gtcccatttc cagatgtttg acgatcaccg
     2281 tcaccgtgtt atcgggcgcg atgcgcagga acgcattcgg catgaattca gcttcggtat
     2341 gcggaccggc ggccgcttcc agccggccgg gcagatacaa accgatcaac agcccgccgg
     2401 cggcggcggc cgacaccttg agaaactcgc ggcgattgag gttcacggat gtgttcatgc
     2461 cacgtccccc ttcgccatca gccgcgccgc gtgtttgatc gcgttacgga tgcgcacgta
     2521 ggtaccgcag cggcacaaca cgccgctcat ggcctcgtcg atgtcctgat cggtcggttt
     2581 gggtttggcg ctgagcagcg ccaccgccga gagaatctgg cccgactggc aatagccgca
     2641 ctgcggaacg tcttcggcga tccaggcccg ttgcacgggg tgagcgtgtt ccggcgacag
     2701 gccttcgatg gtagtaattt ttttgccgct tacagacgag gcaggggtga tacagctgcg
     2761 caccggttgt ccgtcgatga ggacggtaca cgcaccgcac gcggcgatgc cgcaaccgta
     2821 tttcgtgccg gtgagaccga catgatcgcg caacacccag agcaggggtg tgtcggcggc
     2881 aacgtccacc tcctgctgtt ttccgttgac aagaaggttg atcatggctg cctcctatcc
     2941 ttggtgggta ccgggccaga cattcatggt agaacggccg cgcggccgcg acaactggct
     3001 ggcgggaaac caagagtcgg cgcaggttaa aggggtcggg ctaaagctgt ctgttactgc
     3061 gaacctataa taaagcgata attgccacca tacatttgag cgagaaggat ctgtgcatga
     3121 acgaacgact gaccgaactg gaagtgcgcg tggcgtttca ggacaagacc atccaggacc
     3181 tgaacgaggt ggtgacgctt cagcaacgcc agattgaccg gctgacgcag gaactggaag
     3241 cagtaaaatc ccggctgtct gcgctcgcgc cctcgatggt catcccacag gaagatgaga
     3301 aaccgcctcc gcattactga agatttgccc gtttctccgg gattcctgct acaatgcctg
     3361 catcggcgca caccggtctg caccgatccc ttcgcggtca tccatctcgg ttcacctcga
     3421 acaactcaac atatttaggt agcgcctaga ccggcccagt tttttgggta ggccatccag
     3481 gagcaaccat tcgttatgtc attttcatct ctcggcctgt cggccgaatt actccgtgct
     3541 gtgtccgaaa aaggctatac ccaggccacc ccgattcaat tgcaggccat ccccgtcatt
     3601 ctcgagggac gcgatatcct cgccggcgcg cagaccggca ccggcaagac cgccggcttc
     3661 accctgccgc tgctgcaacg gctcacggcg ggcgccaata caagcgcctc gcctgcgcgc
     3721 catcccgtta gagcgctaat cgtggtgccg acccgcgagc tcgcggcgca ggtgcaggaa
     3781 agcgtccgca cctacggcag gcacctcaag gtgcgctcga ccgtgatctt cggcggcgtc
     3841 ggcttcaacc cgcaggcgat
//
LOCUS       DBNGZE010000500         6061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1804617, whole genome shotgun sequence.
ACCESSION   DBNGZE010000500 DBNGZE010000000
VERSION     DBNGZE010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1804617"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC16FV_12825"
     CDS             <1..583
                     /locus_tag="AC16FV_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2850969.1"
                     /translation="LTAPGATPDFRQLVEQTPDLLQTRAQERSAVAGVTLARAGFFPS
                     VDLTGSVAREGQEWTPDKDRRTVGLNLTLPLFSGGKDYYSTKSASASLEAASSNKDNV
                     ERQLLVRLKQAYASYVESVEKLKVDQAFLEAAVTRAEIARSRYNNGLMSFEDWDRIEN
                     DLIQRQKTFLQSQRDRVTAEAAWEQTQGKGVIP"
     gene            586..1551
                     /locus_tag="AC16FV_12830"
     CDS             586..1551
                     /locus_tag="AC16FV_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2850970.1"
                     /translation="MTETARKYRWLIGVAILIAFGTGIYFYWYAGGEAKPAYREVNVT
                     RGDLEVTILSTGVVQPRNRLEIKPPIAGRAEQVLVQEGQSVTKGQIMAWMSSTERAAL
                     IDAARARGPEELKRWEELYRATPVLAPISGTVILRSVEPGQTFTGSDAVFVMSDRLTV
                     KAQVDETDIAQIRLRQPAQLVLDAYPDRPFAGRVDQIAYDAKTVNNVTTYEVDLVPDQ
                     TPAFMRSGMTANVSFVMDTRRNVLLVPNAAVKSRNGDSYVLLASAKPNGTPVEKRIKT
                     GLSDGKHTEVLEGVTENEKLLAPQLQLGGRGETASSPLMPFGRRR"
     gene            1749..3695
                     /locus_tag="AC16FV_12835"
     CDS             1749..3695
                     /locus_tag="AC16FV_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2850971.1"
                     /translation="MIELKDIHKTYRMGQTQVQALRGVSLTIEPGEFVAITGPSGSGK
                     STLMHVIGLLDVPESGSYRLLGKETAHLPEDELAVLRREAIGFVFQQFNLLPRVSASE
                     NVALPLLYSRHELDLTRARTLLAQVGLKDRVNHKPNELSGGQQQRVAIARALVNEPRI
                     ILADEPTGNLDTASQHEIMAILRELNRQGITVVIVTHEEEVAREARRRIRMRDGVIQS
                     DDRIQATPQREAPATVTRTTDRWRLKEIGEHLHQGLRALAANKVRTALSMLGILIGVA
                     AVVAMLALGKGAQAAIKSSLSSLGSNLLVLRQGALRIGGVAQEAGATTRLTIDDVAAI
                     RENVTGVKDVSPAVSGRARVSFGNKNWNTQVLGTGPEYEHMRAAQPGIGRFFTEAENQ
                     KRARVALVGLTVVRELFGNQNPVGEYIKLNKINFQVIGVLPEKGATGWRDQDDIIVIP
                     VSTAMYRLLGKNYVDYIDIEAASSDDLEPVSEAVTQHMLSRHKIPPSQREDAFDIRNL
                     ADIQAALSESSRTMSFLLAAIAGISLLVGGIGIMNIMLVSVTERTREIGLRKAVGARR
                     RDILSQFLVETLVVSLIGGLAGIGLGWIMTVMMSSFAGWSATVSPDAVLLAFLFSAGI
                     GVVFGIYPARKAAGLNPIDALRYE"
     gene            3881..5044
                     /gene="sucC"
                     /locus_tag="AC16FV_12840"
     CDS             3881..5044
                     /gene="sucC"
                     /locus_tag="AC16FV_12840"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR2850972.1"
                     /translation="MNLHEYQAKTLLAEYGVPVPPGRPAFSVSEALEAADELGGDNWV
                     VKAQIHGGGRGKAGGVKKVIGRDALEKVANGLLGSRLVTHQTGPAGQPVHRLLIEKPS
                     VVKRELYLACLLDRALERVVFIASSAGGMDIEEIAARHPEKIMKVVADPVAGLQGYQC
                     REIGFTLGLKDNQITALSRLMLSLYHLYCARDLNLVEINPLAIGGDDELIALDAKIQV
                     DDNALYRQKALEEWRDPSQDDPKEHRAHEFGLNYIALEGNIGCMVNGAGLAMATMDLI
                     KLHGGMPANFLDVGGGTTADKVAEAFKLILSDKNVKAILVNIFGGIVRCDLIAEGIIK
                     AVQEVGIEIPVVVRLEGTNVEKGRALLKHSGLKIISADSLTSAAKQAVAAAKK"
     gene            5041..5913
                     /gene="sucD"
                     /locus_tag="AC16FV_12845"
     CDS             5041..5913
                     /gene="sucD"
                     /locus_tag="AC16FV_12845"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014914674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR2850973.1"
                     /translation="MSILVNKNTKVICQGFTGKQGTFHSEQAIAYGTKMVGGVTPGKG
                     GQTHLGLPVFNTVREAVHVTGATATMIYVPAPLAADSILEAAAVGIKVIACITEGIPV
                     RDMRKVKVALHQYSGVALIGPNCPGIITPGECKIGIMPGFIHKPGSVGIISRSGTLTY
                     EAVYQTTLEELGQSTCVGIGGDPIQGLNFVDCLRLFEADPATRGIVMVGEIGGQAEED
                     AAAYIKNHVTKPVVAYIAGVTAPKGKRMGHAGAVIAGGRGTAEEKYRALEAAGVHTVR
                     SPADIGARMREVLA"
BASE COUNT         1236 a         1818 c         1962 g         1045 t
ORIGIN      
        1 gctgaccgca ccgggcgcga cgccggattt ccggcagctg gtggaacaaa cacccgatct
       61 gttgcagacg cgagcgcagg aaagatcggc ggtagcgggc gtgacgctcg cgcgtgcagg
      121 gttcttcccc agcgtcgatc tcaccggtag cgtcgcccgc gaaggccaag agtggactcc
      181 cgacaaggac cggcgcacgg tgggattgaa tctgaccctg ccgctcttca gcggcggcaa
      241 ggattactac tcgacgaaaa gcgcgtccgc ctcgctcgag gccgcttcgt caaacaagga
      301 caacgtggaa cgtcagttgc tggtacggct caagcaggcc tatgcgagct atgtcgaatc
      361 ggtggaaaag ctcaaggtgg atcaggcgtt cctcgaggcc gccgtcacgc gcgccgagat
      421 cgcgcgctcg cgctacaaca acggtctcat gtcgttcgag gactgggatc gcatcgagaa
      481 cgatctgatt cagcgccaga aaacttttct gcagagccag cgtgaccgcg tcacggccga
      541 ggcagcctgg gagcagacgc aaggcaaagg ggtgattccg tgagcatgac tgaaaccgcg
      601 cgcaagtacc ggtggctgat tggggtggcg atactcattg cgttcggtac cggcatatat
      661 ttttattggt acgccggcgg tgaagcgaaa cccgcttatc gcgaggtcaa tgtgacccgc
      721 ggcgacctcg aggtgaccat actcagcact ggcgtggtgc agccccgcaa tcgccttgaa
      781 ataaaaccgc cgatcgcggg acgcgccgag caggtattgg tgcaggaggg tcagagcgtg
      841 accaagggcc agatcatggc gtggatgagc tcgaccgagc gcgcggcgct gatcgacgcg
      901 gcccgcgccc ggggaccgga agagctcaag cgttgggagg aactgtatcg cgccacgccg
      961 gtgctggcgc cgatcagcgg cacggtgatt ctgcgcagcg tggagcccgg acagaccttc
     1021 accggcagcg atgccgtgtt cgtcatgtcc gaccggctca cggtcaaggc gcaggtggac
     1081 gagaccgaca tcgcgcagat tcgcctgcgc cagccggcgc aactggtgct cgacgcctat
     1141 ccggaccggc cctttgccgg acgcgtggac cagatcgcct atgacgccaa gaccgtgaac
     1201 aacgtcacta cctatgaagt cgatctggtc ccggatcaga caccggcctt catgcgcagc
     1261 ggcatgaccg ccaacgtaag tttcgtgatg gatacgcgcc gcaacgtgct gctggttcct
     1321 aacgcggcgg tgaaatcacg caacggagat tcctatgtcc tgcttgcatc cgcgaagcca
     1381 aatggcacgc cggtcgagaa acggatcaag accggcctga gcgacggcaa gcataccgag
     1441 gtgttggaag gcgtaaccga gaacgagaag ctgctggccc cgcagttgca gttgggtggg
     1501 cgcggggaga cggcatcgag tccgctgatg ccgtttgggc ggagacggtg aatattgtgt
     1561 cgcctccggc gacgttaaaa gctaattgcg ggtgtctccg gtctatacac acgagcaaag
     1621 aaaaggaacc tgccgtgggt cagccaccca caagtacacg tcgccgaagg cgacacaaaa
     1681 aacagtgcag gggcggagcc acccgcaatt agcttttgaa tcgtgcgcgc agcgcacaca
     1741 atagatgaat gatcgaactt aaagatatcc acaaaaccta ccgcatgggc caaacccagg
     1801 tacaggccct ccgcggcgtg tcgctcacga tcgagcccgg cgaattcgtg gctatcactg
     1861 gcccatccgg ttcgggcaag tccaccctca tgcacgtgat cggcctgcta gatgtgcccg
     1921 agtcgggttc ctaccgcctg ctgggcaagg agacggcaca tctcccggaa gacgagctgg
     1981 ctgtgttgcg ccgcgaggca atcggtttcg tgttccagca attcaatttg ttgccgcgcg
     2041 tcagcgccag cgagaacgtg gcgctgccgc tgttgtactc gcggcacgag ctggacctga
     2101 cgcgggcccg gacattgctc gcacaggtgg gactcaagga tcgcgtgaac cataaaccga
     2161 acgaactctc cggcggccag cagcagcgcg tcgccattgc gcgtgccctc gtcaacgagc
     2221 cacgcatcat tctggccgat gaaccgaccg gaaatctgga taccgccagc cagcacgaga
     2281 tcatggcgat tctgcgtgaa ctcaaccggc agggcatcac tgtcgtgata gttacgcacg
     2341 aagaggaagt cgcgcgcgag gcgcgccggc gcatccgcat gcgcgatggc gtgatccagt
     2401 ccgacgatcg catacaggca acgccgcaac gcgaggcgcc agcgacggtg actaggacca
     2461 cggatcgctg gcgcctcaag gagattggcg agcatttgca tcagggcctg cgcgccctcg
     2521 cggccaacaa ggtgcgcacc gcgctgtcga tgctgggtat cctgatcggc gtggcggcgg
     2581 tggtggcgat gctggcactc ggcaagggcg cccaggcggc catcaaaagc agtctctctt
     2641 cgctgggatc gaacctgctt gtgctgcgcc agggcgcgtt gcgcatcggc ggcgtggcgc
     2701 aggaagcggg cgccacgacg cggcttacca tcgacgatgt ggcggcgatc cgggaaaacg
     2761 tcacgggcgt gaaagacgtg tcgcccgcgg tctcgggacg cgcgcgcgtc agcttcggta
     2821 acaaaaactg gaacacccag gtgctgggca ccggccctga atacgagcac atgcgcgcgg
     2881 cacaacccgg tatcgggcgt ttcttcacgg aggcggaaaa ccagaaacgc gcgcgcgtgg
     2941 cgcttgtcgg actcacggtt gtgcgcgagt tgttcggcaa ccagaatccg gtgggtgaat
     3001 acatcaagct caacaagatc aattttcagg tcatcggcgt gctgcccgag aagggcgcaa
     3061 ccggctggcg cgatcaggac gacatcatcg tgatacccgt gagtacggcc atgtaccggc
     3121 tcctcggcaa gaactacgtg gattacatcg acatcgaggc ggcaagtagc gacgatctcg
     3181 aaccggtcag cgaggcggtg acgcaacata tgttgtcgcg tcacaagatc ccgccgtcgc
     3241 agcgcgagga tgccttcgat atccgcaatc tcgccgacat ccaggcagcg ctctcggaaa
     3301 gcagccgcac catgtcgttt ctgctcgccg ccatcgccgg tatttccctg ctggtgggcg
     3361 gcatcggcat catgaacatc atgctggtgt cggtgaccga gcgcacgcgc gagatcggcc
     3421 tgcgcaaggc cgtgggtgcg cgccgccgcg atatcctttc gcagttcctg gtggaaaccc
     3481 tggtggtgag cctgatcggc gggctcgccg gcattggcct cggctggatc atgacggtga
     3541 tgatgtcgtc attcgccggc tggtcggcga cggtctcgcc ggacgccgtg ctgctggcgt
     3601 tcctgttctc cgccggcatc ggcgttgtct tcggcattta tcccgcgcgc aaggcggccg
     3661 gcctcaaccc catcgacgcg ctgcgctacg aataggccag tctgcgcatg atgtgctaaa
     3721 ttgcgtgtca tattttaata tttggaaccg cagataaacg cggatgaacg cagataaaat
     3781 gcaagaaaat aaacggttgg ttttgtatta atccgcgtta atctgcgttc atctgcggtt
     3841 tcgacttatt tttaataatc ataaagaact ggcgtaaccc atgaacctgc acgaatacca
     3901 ggccaagacc ctgctcgccg agtacggcgt gccggttccc ccaggccggc ccgccttctc
     3961 ggtgagcgag gcgctggagg cggcggacga actgggcggc gacaactggg tggtgaaggc
     4021 gcagattcac ggcggcggtc gcggcaaggc cggcggcgtg aaaaaggtca tcggccgtga
     4081 cgcgctggag aaggttgcca acggtttgct cggctcgcgc ctcgtgacgc accagaccgg
     4141 tcccgccggg caaccggtgc accgcctgtt gatcgaaaaa ccgagcgtgg tgaagcgtga
     4201 gctgtatctg gcctgcctgc tggatcgcgc gcttgaacga gtcgtcttta tcgcctccag
     4261 tgccggcggc atggacatcg aggagattgc cgccaggcac ccggaaaaga tcatgaaagt
     4321 ggtggccgat cccgtggccg gcctgcaagg ctaccagtgc cgcgaaatcg gtttcacgct
     4381 gggactgaag gacaatcaga tcacggcgct gtcgcgtctg atgctgagcc tctatcacct
     4441 ctactgcgcg cgcgacctga atctggtgga gatcaatccg ctcgcaatcg gcggcgacga
     4501 cgagctgatc gcgctcgacg ccaaaatcca ggtggacgac aacgcgctct accgccagaa
     4561 ggcgctggaa gagtggcgcg atccctcgca ggacgatccc aaggagcacc gcgcgcacga
     4621 attcggcctc aattacattg cgctcgaggg caacatcggc tgcatggtga acggcgccgg
     4681 actggctatg gcgaccatgg acctgatcaa gctgcatggt ggcatgccgg cgaacttcct
     4741 cgacgtcggc ggcggcacca cggccgacaa ggtggccgag gccttcaagc tgattctttc
     4801 ggacaaaaat gtaaaagcga tcctggtcaa catcttcggc ggcattgtgc gttgcgacct
     4861 gatcgccgag ggcatcatca aggcggtgca ggaagttggc atcgagattc cggtggtggt
     4921 gcgtttggaa ggcaccaacg tcgagaaagg gcgtgcgctc ctgaagcaca gtggtctgaa
     4981 aatcatttcg gcggacagcc tgaccagcgc cgccaaacag gcagtcgcgg cggcaaagaa
     5041 atgagcatac tggttaacaa gaatacaaag gtgatttgcc agggcttcac cggcaagcag
     5101 ggcacgttcc attccgagca ggcgatcgcc tacggcacca aaatggtcgg cggcgtgacg
     5161 ccgggcaagg gcggacaaac gcatcttgga ttgccggtgt tcaacaccgt gcgcgaggcg
     5221 gtgcatgtca ccggcgcgac cgcgaccatg atttacgtgc cagcgccgtt agccgccgat
     5281 tccatcctgg aagcggccgc ggtcggcatc aaggtgatcg cgtgcatcac cgagggcata
     5341 ccggtgcgcg acatgcgcaa ggtcaaggtt gcgctgcatc agtattccgg cgtggcgctc
     5401 atcggaccga actgccccgg catcatcacg ccgggcgagt gcaagattgg catcatgccc
     5461 ggcttcattc acaagcccgg gagcgtcggc atcatttcgc gctccggcac gctgacctac
     5521 gaggccgtgt atcaaacgac gctggaagag ctggggcaga gcacctgcgt cggcatcggc
     5581 ggcgatccga tccaggggct gaatttcgtg gactgcctgc gtctgttcga ggccgatccc
     5641 gccacccgcg gcatcgtcat ggtgggcgaa atcggcggac aggcggagga agacgccgct
     5701 gcctatataa agaatcacgt gaccaagccg gtggtggcgt acatcgccgg ggtgaccgcg
     5761 cccaagggca agcgcatggg tcacgccggc gccgtgattg ccggtggcag gggtacggcc
     5821 gaggaaaaat accgcgcact cgaggccgcc ggtgtgcaca ccgtgcgttc tcccgcggac
     5881 atcggcgcgc gcatgcgcga agtgctggcc tgacatgggc gcgcgcctgc gcatcgccct
     5941 cggccagctc aacctgctgg tgggcgatgt cgccggcaat gccgcgaaga tgatcgccgc
     6001 ggcacagcgc gcgcgcgacg aactcaaggc cgatgtcatt ctctttcccg aactcgcgct
     6061 c
//
LOCUS       DBNGZE010000501         1762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1804740, whole genome shotgun sequence.
ACCESSION   DBNGZE010000501 DBNGZE010000000
VERSION     DBNGZE010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1804740"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16FV_12850"
     CDS             complement(<1..180)
                     /locus_tag="AC16FV_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2850974.1"
                     /translation="MAGDFVKDKKSLPKRAPYLNRSLLSVHITTWGRDYSHRQTGSFR
                     DHASEEIHERQGPIYF"
     gene            226..1683
                     /locus_tag="AC16FV_12855"
     CDS             226..1683
                     /locus_tag="AC16FV_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin-like domain-containing protein"
                     /protein_id="HIR2850975.1"
                     /translation="MKGGVGFYIVGAGLLLALAGCGRSFMSGERAAWRHQAEVECLKS
                     GSVKIGIGVVQIEPIEGPGMCGADFPLKVSALGESAPAIGYADDPRPPGSIPNSSAQM
                     PRWPISEPQDVPPAPIQAAPMPGQSTQAPRMRWVPGAPPAERSNSVAPGGQPISLSAP
                     GAEPGVTPSAAPLPNDIPDDAVLPPGRASNTYSPQRVYGAPVYQLPQRDYGGPVYQSP
                     QRASPPLGPTRGRQTVANITPATLTPPATLACPIVSALDHWVGEGVQPAAMRWFGTPV
                     TEIKQISAYSCRGMVGAGGSGISEHAFGNAIDIAAFTFADGRRVTVQEGWHGTPEEQG
                     FLRDVHLAACDNFTTVLAPGYNAAHYNHIHVDLMRRSSGERPCRPEAISGELAAAKAR
                     AVYAGRQRGPAFTGAVQKNVQKSFSPAAVPGADGYLLDADDVLLGLKPAVQRHGAATD
                     IELNGDDITGTVVPTASPRATSKKIKSTDDRLTAH"
BASE COUNT          333 a          542 c          546 g          341 t
ORIGIN      
        1 aaaatatatc gggccttgcc tttcatgaat ctcctcagat gcatgatccc gaaaagaacc
       61 ggtttgccga tgagaataat cgcgtcccca cgtagtgatg tgaaccgaaa gcagcgagcg
      121 gttcaagtag ggggcgcgtt ttggcaagga tttcttatcc ttcacgaaat cgccggccat
      181 aggcgttgaa gcctcgttaa agaaactgct cgcatttttg cgggtatgaa gggtggcgtg
      241 gggttttata tcgtcggcgc aggtctgttg ctggcgcttg ccggctgcgg gcgcagcttc
      301 atgtctggcg aacgggcggc ctggcggcac caagccgagg ttgaatgttt gaagtcgggt
      361 tccgtcaaga tcggcatcgg ggtcgttcag atcgagccaa tcgaaggccc gggcatgtgt
      421 ggtgccgatt ttccgctcaa ggtctcggcc ctgggcgaga gcgcgccggc catcggttat
      481 gcagatgatc ctcgtccgcc cggatcgatt cccaacagtt ccgcacagat gccgcgctgg
      541 ccaatcagtg agccgcaaga cgtgccgcct gcacccatac aagccgcgcc gatgccaggg
      601 cagtccacgc aagcgccacg aatgcgctgg gtgccgggag cgcccccagc cgaacgatcc
      661 aattccgtcg cgccgggcgg tcagccgatt tcactgagcg cgccgggcgc cgagcccggc
      721 gttacgccga gcgcagcgcc attacccaat gatattcccg acgatgccgt gctgccgcca
      781 ggccgagcgt ccaacacgta ttcgccgcag cgcgtctatg gtgcgccggt ctatcagctg
      841 ccgcagcgcg actatggcgg cccggtttat cagtcgccac agcgtgcgtc gccaccgctc
      901 gggcccacac gcggccggca aacggtggct aacatcacgc ccgcgactct cacgccgccc
      961 gcgacactgg cttgtccgat cgtctccgca ctcgaccact gggtgggcga gggcgtgcaa
     1021 cctgcggcga tgcgctggtt tggcacgccg gtcaccgaga ttaagcaaat ctccgcctat
     1081 tcatgccgtg gcatggttgg cgccggtggt tcgggaatat ccgaacatgc gttcggcaat
     1141 gcgatcgaca ttgctgcctt cacgtttgcc gatggccgcc gggttacggt gcaggagggc
     1201 tggcatggca cgccggaaga gcagggcttc ctgcgcgacg ttcatcttgc ggcctgcgat
     1261 aattttacga cagtgctggc ccctggctac aacgccgccc actacaatca catccatgtc
     1321 gacctgatgc gccgttcgag cggagagcgt ccttgtcggc cggaggcgat ttccggcgaa
     1381 ctggcggcag caaaagcccg ggcggtctat gccggcaggc agcgcggtcc cgcatttaca
     1441 ggtgcagtcc agaagaacgt ccagaagagc ttttcgccgg cggcagtacc tggcgcggac
     1501 ggttatcttc tggatgcaga cgatgtgttg cttggtttga agcctgctgt ccaacgccat
     1561 ggcgccgcta ccgacatcga attgaacggc gacgacatta ccggcacggt tgtgcctacg
     1621 gcgtcaccgc gcgcaacttc caagaaaatc aagtccactg atgaccgctt gaccgcccac
     1681 tagaccgccg cgtcgatcag catcgcgccg aacagaataa ggccggcatc gcgattggat
     1741 ttgaacagca ttaggcagtg tt
//
LOCUS       DBNGZE010000502        10172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_694123, whole genome shotgun sequence.
ACCESSION   DBNGZE010000502 DBNGZE010000000
VERSION     DBNGZE010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_694123"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..610
                     /locus_tag="AC16FV_12860"
                     /pseudo
     CDS             1..610
                     /locus_tag="AC16FV_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639714.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
     gene            complement(623..1141)
                     /locus_tag="AC16FV_12865"
     CDS             complement(623..1141)
                     /locus_tag="AC16FV_12865"
                     /inference="COORDINATES: protein motif:HMM:NF020965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HIR2850976.1"
                     /translation="MSIPRVPCIRFKPILLVAGMLLYPCSAGADMLAVAVGHGNMSSQ
                     GSTAVFFSYQKDAPILFNQKSSYELTIAYWSGRNYNTALTAARTLRWNLSPENYFAGT
                     LGIGMVDRTTDHLGTTGQFVARLAFGRKLGKYDLSIGETHYSNGKTALRLGWHGPNIG
                     EDFLTLMLAREF"
     gene            1308..1880
                     /locus_tag="AC16FV_12870"
     CDS             1308..1880
                     /locus_tag="AC16FV_12870"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase family protein"
                     /protein_id="HIR2850977.1"
                     /translation="MPIVSPVAPQCTEPLWRPAHRLSRAGIPEKYLPWLLDPASLTER
                     IIAHCRENFRVRLLDQRRMRPLRNETEALGMRAGTRAIVRQVQLVCGDTPWVYARTII
                     PPRTFARKSHRFTTLGARSLGAMLFADPSMKRGEVEVARLTPSDRLYHLATRELRDKP
                     GTIWGRRSLFHLGGKPLLVCEFFLPDIAEF"
     gene            1883..2746
                     /gene="ubiA"
                     /locus_tag="AC16FV_12875"
     CDS             1883..2746
                     /gene="ubiA"
                     /locus_tag="AC16FV_12875"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR2850978.1"
                     /translation="MIVAARLMDYARLMRLHRPIGILLLLWPTLWALWIAGHGRPDSH
                     VFIVFVLGVVLMRSAGCVINDYADRDFDPHVERTRDRPIAAGRVSPREALVLFSVLCL
                     IAFALVLTLSRLTILLSFAGAFLAATYPFLKRYTHLPQFYLGMAFGWGIPMAFAAQTG
                     GVPALAWVLFAANICWSVAYDTAYAMVDREDDRKVGVKSTAILFGRYDRAMVFLFHIM
                     TIALLVFVGALAGLGLGYYAGLAAALGFALYEQWLMRDRDRDGCFRAFLNNNWLGAAV
                     FAGLLWHYYGR"
     gene            2751..3365
                     /locus_tag="AC16FV_12880"
     CDS             2751..3365
                     /locus_tag="AC16FV_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR2850979.1"
                     /translation="MKLYGSLTSPYVRKARILIREKDLSCEFIVADAWAADSPIPALN
                     PLGKVPVLALDNGEVLFDSPVIVEYLDALKAPALLAASGEARWNILRWEALADGVLDA
                     VVTRLLESRRPIAQQSADNIRRQEEKVARSLEYTARRLGNGPWLASDRFTLADLVMAV
                     ALEYTDFRYPHDWRGRYPRLGQWLAGVSARPSFVETRPPGTEKK"
     gene            3383..4396
                     /locus_tag="AC16FV_12885"
     CDS             3383..4396
                     /locus_tag="AC16FV_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR2850980.1"
                     /translation="MTIGLLFVALLVILIGAETFTNALEHLGERLKISEGVTGSIFAA
                     VGTALPETMVPVVAVLSTASTQQVREEVGVGAILGAPLMLSTLTLFLMALFAIHKRGW
                     SDELHPERTGLRRDLSWFLLAFGLSTVAIFIPHHSTGMRALIAISLVLVYFIYLMLTI
                     RASAKLVADGHGTAASEPLFLVRLFGRAGMPENMFTILLQLATGLTLIIFGAHGFVQG
                     VEQLSVWLGISALVLALLIVPIATELPEKMNSILWIRKRKDTLAFGNVTGAMVFQGSL
                     LPALGILLTPWEPRPEVLAGVILTLVASAYLLFMARRGHLRPVHLFFNGLCYVTYLMT
                     VLL"
     gene            4460..5308
                     /gene="aroE"
                     /locus_tag="AC16FV_12890"
     CDS             4460..5308
                     /gene="aroE"
                     /locus_tag="AC16FV_12890"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR2850981.1"
                     /translation="MATQAEKDATPARYAVMGNPVAHSKSPVIHKQFAHQFGHNIEYT
                     ALWVDTDGFADAVEQFRAEGGKGLNVTVPFKLEAFSLADSLSDRARLAGAVNTIRFET
                     DGKIFGDNTDGTGLVHDLTKNLNVHLRGKKILMLGAGGAVRGALGPLLKQNPAVLVIA
                     NRTVSKAKELAKSFAQFGKVEAVGYDELVGKRFYIVINGTSASLKGEMPPLPVNLFAS
                     NAVAYDMMYGDKPTPFLEWAMLHGAETAADGLGMLVEQAAESYLLWRGVRPETKHVIA
                     VLRKGG"
     gene            complement(5325..5489)
                     /locus_tag="AC16FV_12895"
     CDS             complement(5325..5489)
                     /locus_tag="AC16FV_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2850982.1"
                     /translation="MAKSFRKLDPLKVRKLKEQGLTNAQIAQRLGVTQGAIYHVLRKT
                     TSSLGKALAI"
     gene            complement(5547..5819)
                     /locus_tag="AC16FV_12900"
     CDS             complement(5547..5819)
                     /locus_tag="AC16FV_12900"
                     /inference="COORDINATES: protein motif:HMM:NF025073.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2850983.1"
                     /translation="MDYLDLKFALARKGYTLTRVAEELGLFGPQSVQQVLTRKYISAR
                     VERRVAEIIGIPLEKLFPDRYQSAKRRRRNNNANLHEKTRDTGCTS"
     gene            5892..6356
                     /locus_tag="AC16FV_12905"
     CDS             5892..6356
                     /locus_tag="AC16FV_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR2850984.1"
                     /translation="MVNKITPVNTGGAINSPPARLKAERHRLGLSQVALGKALGVTKW
                     TIINYERTSGRGTPIPADLLSGCSRLGMDVQYIITGVSSSNLNRVAEEAGSYRAEPKR
                     PSALSKEEQQLLEKYRRLKPSQRTHAQTIVEALISAGGKAVKKPASRPRRKR"
     gene            complement(6369..6896)
                     /locus_tag="AC16FV_12910"
     CDS             complement(6369..6896)
                     /locus_tag="AC16FV_12910"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR2850985.1"
                     /translation="MAIRPYKGILPTVGPRVYIDEAASIIGDVVVGEDTSIWPMCSVR
                     GDVNYIRIGARTNIQDGSILHGTHDYEGVPGGWAVVVGDDVTIGHQCIIHGCTVESRC
                     LIGMGSAILDGAVLRGGVFLGAGSLVTEGKELEGGYLWMGRPVKRVRELTAEEKTWFD
                     YSARHYVKLKNDYLK"
     gene            complement(6926..7921)
                     /locus_tag="AC16FV_12915"
     CDS             complement(6926..7921)
                     /locus_tag="AC16FV_12915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR2850986.1"
                     /translation="MTAEAPHPYDSLTPEIILEAVELYGVRCTGGLLALNSYENRVYR
                     VDTEDGPPCVAKFYRPGRWDEAAILEEHTFAQQLAEHEIPAVAPLRRHDGNTLSYHGG
                     FRFAVFPWQPGRAPELNSREDRALLGRYLGRLHRLGAAEPFRHRARLGIEEFGHRSVR
                     YLKEHDIVPEELRASFFAIMEYLFPPIEAAFRAVDDAVWIRLHGDCHLGNILWTGTGP
                     HLVDFDDCLTGPAVQDLWMLLSGNREDMELQLADVLSGYTEFMEFNSAELRLMEPLRT
                     LRMLRYMAWLAERWDDPAFPRNFPWFNTRRYWEEQVLALKQQAAALDELPLSWMA"
     gene            7946..10003
                     /gene="prlC"
                     /locus_tag="AC16FV_12920"
     CDS             7946..10003
                     /gene="prlC"
                     /locus_tag="AC16FV_12920"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HIR2850987.1"
                     /translation="MENTSLTSNPLLDLAQPPRFGAILPEHAEPALDHVLAENRAALE
                     RLLASGGPYTWDNFAQPIEDMRERLARLWSPVSHLHAVMDSEALRAAYNAGLPKLTAY
                     FTELAQDERLYAGYKSIAAGPEFPKLTQAQRKIIENTLRDFRLAGAELPPKEKARLKA
                     VQQELAALASKFSENVLDATQAWDLRISDEKDLAGLPESARAMARQDAQEKNLEGWHF
                     TLEGPSYIAFMTYSDDRERRRQMYEAFVTRASDQGPTAGKWDNSELILKLLRLRREAA
                     RLLGFGNFAEYALQTRMAKKVPEVMDFLNDLARRAKPAAQRDFDELKQFAREAHGVER
                     LEAWDIAYYSEKLQQAKYRISQEDLRPYFPETKVVPGMFEVVERLYGLKVSAVKDVEV
                     WHPDVRFYEIRDGAGEVRGRFYMDLYARANKRGGAWMDECINRKRTTGGVQVPVAYLV
                     CNFTPPVAGRPALFTHDEVITLFHEFGHGLHHMLTRIDYVGVAGINGVAWDAVELPSQ
                     FMENWCWEREALNLIARHHESGAKIPDELYAKMIAAKNFQSGMQFVRQLEFSLFDMRL
                     HGAYDPDGAGTVQQVLDEVRAEVAVVIPPAFNRFQNGFSHIFAGGYAAGYYSYKWAEV
                     LSADAFSKFEENGVFDRATGLQFLQNILEQGGSREPMELFMKFRGRAPKIDALLRHSG
                     LAA"
BASE COUNT         1871 a         3087 c         3226 g         1988 t
ORIGIN      
        1 cagggatggc gcgcgtcaga ccttagagct tgagacggca acggatacgg cgcaggcatt
       61 gcaggaggcg ttgcccgaac tgcggatcgc gctgatacac gggcgcatga agccgccgga
      121 aaaggaaaag atcatggcag cgttcaagcg cggtgacgtg catttactgg tcgccaccac
      181 cgtcatcgag gttggtgtgg acgtgcccaa cgcctcgctc atggtgatcg agaacgccga
      241 gcggctgggt ttgtagcaac tacatcagtt gcgcgggcgc gtcggccgcg gcgcggcgga
      301 gagccattgt gtgctgctgt atcagccgcc gttgtcggaa atggcgcggg cacggctggc
      361 ggcgctgcgc gagaccagca acggcttcga gatcgcgcgg cgcgatctcg agatgcgcgg
      421 gccaggcgag atgctgggca cgcgccaagc cggcatgccg cagtttcaca ttgccgatct
      481 gctgcgcgat cgcgcattgc tgccgcgcgt gcaacaggtg gcggagctgt tgctcaagga
      541 acatccgcaa tgcgtggacc cgatcgtgcg gcgctggatc agcggcgccg agagctatgg
      601 caacgtttga agcacccgcg cgctagaatt cgcgcgccag catcagcgtc aggaaatcct
      661 ccccgatgtt cggcccatgc cagccgagcc gcaaggcggt tttgccgttc gagtaatggg
      721 tttcgccgat ggacagatcg tattttccaa gcttgcgccc gaacgcgagg cgcgccacga
      781 attgtccggt agtgcccaga tgatccgtgg tccggtcaac catgccgata ccgagcgtgc
      841 ccgcgaaata attctccgga gaaagattcc agcgcagcgt gcgcgcggcg gtgagcgcgg
      901 tgttgtaatt ccgaccgctc cagtaggcga tggtcaattc gtaggaactt ttttgattga
      961 ataatattgg cgcgtctttc tggtacgaaa aaaacacggc ggttgagcct tgcgaggaca
     1021 tgtttccatg ccccaccgcg acagccagca tgtcggcgcc ggcggaacat ggatataaca
     1081 gcatgccggc aaccagcaga atcggtttga atcgaataca tgggacgcgt ggaatgctca
     1141 tttgattctc cccgaaccgg ataccgtcct gagcattgat cggcgacatt gaggtctttc
     1201 ccgtatgatt cccggaggac gatgtcatgc atagtgtcgc tgcttccgct gaccgcccag
     1261 gacgtttctg ctacgattcg gcacccataa cccattgata acgttgcatg cctattgtga
     1321 gcccggtagc accgcaatgc acggaaccgt tgtggcggcc ggcgcaccgg ctgagccgcg
     1381 ccggcattcc ggaaaaatat ctgccatggt tgctcgaccc ggcgtcgctc acggaacgca
     1441 tcatcgccca ttgccgcgaa aattttcgcg tgcggttgct ggaccagcga cgcatgcgtc
     1501 cgttgcgcaa cgagaccgaa gcgctcggca tgcgcgccgg tacccgtgcg atcgtgcgcc
     1561 aggtgcagct cgtgtgcggc gacacaccct gggtatatgc gcgcaccatc atcccgccgc
     1621 ggacgtttgc cagaaaatcc caccgcttca cgacactggg tgcacgttcc ctcggggcca
     1681 tgctgttcgc cgatccgtcc atgaagcgcg gcgaggtgga ggtcgcgcgt ctcacgccct
     1741 cggatcgtct ctatcatctg gcgacacgcg aactgcgcga caaacccggg acgatctggg
     1801 gacgacgctc gctgttccat ctcggcggca agccgctgct ggtgtgtgaa ttctttctgc
     1861 ctgatatcgc cgaattttaa acatgatcgt ggcggcgcgg ctgatggatt atgcgcggct
     1921 gatgcggctg caccggccga tcggcatctt gctgttgctc tggccaacgc tgtgggcgtt
     1981 gtggatcgcc gggcacggcc ggcccgactc gcatgtcttc attgtgtttg tgcttggcgt
     2041 ggtgctgatg cgttccgccg gttgcgtcat caacgattac gccgaccggg atttcgaccc
     2101 gcatgtggag cgcacgcgtg accggccgat cgccgccgga cgcgtgagcc cgcgcgaggc
     2161 gctcgtgctt ttctctgtcc tgtgcctgat cgcattcgcg ctggtgctca cgctcagtcg
     2221 cctgaccatt ctgctttcct tcgccggcgc gtttctggcc gcgacctatc ctttcctcaa
     2281 gcgttacacc catctgccgc agttttatct cggcatggcc ttcggctggg gcatcccgat
     2341 ggcgttcgcc gcccagaccg gaggtgtgcc ggcgctcgcc tgggttttgt tcgccgccaa
     2401 catatgctgg tccgtggcct acgacaccgc ctatgccatg gtggaccggg aggatgatcg
     2461 caaggttgga gtgaaatcca ccgccatcct gttcggtcgc tacgaccgtg ccatggtgtt
     2521 tctttttcac atcatgacaa tcgccctgct ggtatttgtc ggcgcgctcg ccggtctcgg
     2581 tttggggtat tatgctggcc tcgcggcggc gctggggttc gcgctttatg aacagtggct
     2641 catgcgtgac cgtgatcgcg acggctgctt ccgcgcgttc ctgaacaaca actggctcgg
     2701 cgcggcggtg ttcgccggac tgctgtggca ttactacgga agatgatgcg atgaaactct
     2761 acggctcatt gacgtcgccc tacgtgcgca aggcccgcat actgatccgg gaaaaggatt
     2821 tgtcgtgtga gtttatcgtg gccgacgcgt gggccgcgga cagcccgatt cccgcgctca
     2881 acccgcttgg caaggtgccg gtgctggcgc tcgacaacgg cgaagtgctg ttcgattcac
     2941 cggtgatcgt cgagtatctc gatgcgctca aggcgccggc gttgctggcc gcctcgggtg
     3001 aagcgcgctg gaacatactg cgctgggagg cgctggccga tggtgtgctg gatgcggtgg
     3061 tgacgcgttt gctcgaatcg cgccgaccca tagcgcagca atccgcggac aatatccggc
     3121 gccaggaaga aaaggtcgcg cgttcgctgg aatacaccgc gcggcgtctg ggcaacggcc
     3181 cctggctcgc gtcggaccgt ttcacgctcg ccgatctcgt catggcggtt gcgctggaat
     3241 acaccgactt ccgttacccg cacgactggc gcggccgtta cccgcggctc ggacagtggc
     3301 tggccggtgt cagcgcgcgt ccgtcattcg tcgaaacccg tcctcccggc acggaaaaga
     3361 aataaatcaa ggaatcccgc gcatgactat cggcctcctt ttcgtcgcgc tgctcgtaat
     3421 cctgatcggc gcggagacct tcaccaacgc cttggagcat ctcggcgaac gtctcaaaat
     3481 ctccgagggc gtgaccggtt ccatcttcgc ggcggtcggc accgccctgc ccgagaccat
     3541 ggtgccggtg gtggccgtcc tctcgaccgc gagcacgcag caggtgcgcg aggaggtggg
     3601 cgtgggcgcg attctcggcg cgccactcat gctttcgacg ctgacgttgt ttctgatggc
     3661 gttgttcgcc attcacaagc gcggctggtc ggacgagctg caccccgaac gcaccggtct
     3721 gcggcgtgac ctgtcgtggt tcctgctggc gtttggcctg agcacggtcg ccatattcat
     3781 accgcatcat tcgacgggaa tgcgcgcgct catcgccatt tcgctggtgc tggtttattt
     3841 catttatctc atgctcacca tccgcgcctc ggccaagctc gtcgcggacg ggcatggcac
     3901 cgccgccagc gagccgctgt ttctggtgcg gctgttcggt cgcgccggca tgccggagaa
     3961 catgttcacg atactgctgc agctcgccac gggattgacg ttgatcatct ttggcgcgca
     4021 cggcttcgtg cagggggtcg agcagctgtc cgtctggctc ggcatctcgg cgctggtgct
     4081 ggcgctgctg atcgtcccga tcgccaccga actgccggaa aagatgaaca gcatcctctg
     4141 gatccgcaag cgcaaggata cgctcgcctt cggcaacgtc accggcgcca tggtgtttca
     4201 gggctcgctg ctgccggcgc tcggcatcct gctcacgccg tgggagccgc gcccggaggt
     4261 gctcgccggc gtgatcctga cgctggtggc gagcgcctac ctgctgttca tggcgcgccg
     4321 tggccacctc cggccggtgc atcttttttt caacggcctg tgttacgtca cctacctgat
     4381 gacggtcctg ctctagcccg cgtgccgcgg cgcgggcaat agcctagaat aggcccgcat
     4441 gcatgttcag gatcgacacg tggccaccca agcggaaaaa gacgcaaccc cggcgcgtta
     4501 cgcggtgatg ggcaatcccg tcgcccacag caaatcgccc gtgattcaca aacagttcgc
     4561 ccaccagttc ggccacaata tcgagtacac cgcgttatgg gtcgacaccg acggattcgc
     4621 cgatgcggtc gagcagttcc gcgccgaggg cggcaagggg ctcaatgtca ccgtgccgtt
     4681 caagctcgag gccttcagtc tggcggacag cctgagcgac cgcgcccggc tcgcgggcgc
     4741 ggtcaacacc attcgtttcg agaccgatgg gaaaattttc ggcgacaaca ccgacggcac
     4801 cggcctggtg cacgacctca ccaagaacct gaacgtgcac ctgcggggca agaaaatcct
     4861 gatgctgggc gcgggcggcg ccgtgcgcgg cgcgctcggg ccgctgctca agcagaatcc
     4921 ggccgtgctc gtcatcgcca accgcacggt gtccaaggcc aaggagcttg ccaagtcctt
     4981 cgcacagttc ggcaaggtcg aggccgtcgg ctacgacgag ctcgtcggca agcgtttcta
     5041 catcgtgatc aacggcacca gcgcgagcct gaagggtgaa atgccgccgc tgccggtgaa
     5101 cctcttcgcc agcaacgcgg tggcctacga tatgatgtat ggcgacaagc ccacgccgtt
     5161 cctcgaatgg gccatgctgc acggcgccga aaccgccgcc gacggcctcg gcatgctggt
     5221 ggagcaggcg gcggagtcat acctgctctg gcgcggcgtg cggccggaaa cgaagcacgt
     5281 aattgcggtg ttgcggaagg gcggctgaaa aaacttatgc aggtctatat cgccaaagct
     5341 ttccccagac tcgacgtcgt cttgcgcaag acgtggtaaa tcgcgccctg cgtaacgccc
     5401 agccgttgcg cgatctgggc attcgttaat ccctgttcct tcagtttccg taccttcaac
     5461 gggtcaagct tgcggaatga cttggccaag atttctcccc atcgcctgca tgacgacctc
     5521 gcgcgcggtt tcttcggcgt gacgcttcac gaggtgcagc cggtatcgcg cgttttttcg
     5581 tgtagattcg cgttattgtt gcgtctccgg cgtttcgccg actgataacg gtccggaaag
     5641 agtttctcca gcgggatgcc gatgatctcc gcgacgcgcc gttcgacgcg ggcgctgatg
     5701 tatttgcgtg tcagcacctg ctgcaccgac tgcggtccga acagccccaa ctcctcggcg
     5761 acgcgcgtga gggtgtagcc cttgcgggcg agcgcaaact tcaggtccag ataatccatt
     5821 cttgttattc cgtgtgttag gattcagctg aagatcatca gctttaactt tcctttttct
     5881 ttgagcagag tatggtaaac aaaattacac ctgtcaacac gggcggtgca ataaattcac
     5941 ctcccgcccg gctgaaggcg gagcgccatc ggctcggcct gagccaggtg gcgctgggca
     6001 aggcgctggg ggtgaccaag tggaccatca tcaattacga aaggaccagc gggcgtggca
     6061 cgccgatccc ggcggacctg ctttccggct gttcccggct cgggatggac gtgcagtaca
     6121 tcattaccgg tgtttcctcg agcaacctga accgggtggc ggaggaggcc ggcagttacc
     6181 gggcggagcc gaagcggccg tccgcgctgt cgaaggaaga acagcaactg ctggaaaaat
     6241 accgccgtct gaagccatca caacgcacac atgcgcaaac gattgtggag gccctgatat
     6301 cggcgggagg caaagcagta aaaaaaccgg cctcgcggcc gcgacgaaag cgctgagcgc
     6361 gccccggttt acttcagata atcgtttttg agtttgacgt aatgcctggc tgagtaatcg
     6421 aaccaggtct tttcctcggc cgtcagttcg cgcacccgct tcaccggccg gcccatccac
     6481 agatacccgc cttccagttc cttgccctcc gtgaccagac tgccggcgcc gaggaacacg
     6541 ccgccgcgca gtaccgcgcc gtcgagaatg gccgagccca tgccgatgag gcagcgcgat
     6601 tcgacggtgc agccgtggat gatgcactga tggccgatgg tgacgtcgtc gccgaccacc
     6661 acggcccaac cgcccggcac gccttcataa tcatgcgtgc cgtggagaat gctgccgtcc
     6721 tggatattgg tgcgtgcacc gatgcggatg taattgacat cgccgcgcac cgagcacatg
     6781 ggccagatgg aggtatcctc gccgacgacc acgtcgccga taatgctcgc ggcctcgtcg
     6841 atgtaaaccc ggggcccgac ggtgggcagg atgcctttgt agggacgtat cgccatggcc
     6901 gcgattatag ggagaaaaag ccggcctacg ccatccacga caacggcagt tcatccagcg
     6961 ccgcggcctg ttgcttgagc gcgagcacct gttcctccca gtagcgccgc gtgttgaacc
     7021 aggggaagtt gcgcggaaag gcagggtcgt cccagcgctc ggcgagccag gccatgtagc
     7081 gcagcatacg cagtgtgcgc agcggttcca tcaggcgcag ctcggcagaa ttgaattcca
     7141 tgaattcggt gtagccggac agcacgtcgg caagctggag ctccatgtcc tcacggttgc
     7201 ccgaaagcag catccacaaa tcttgcaccg ccgggccggt gaggcagtcg tcgaaatcca
     7261 ccagatgcgg gccggtgccg gtccagagga tattgccgag gtgacagtcg ccatgcaggc
     7321 gtatccacac ggcatcatcg acggcccgga aagcggcctc gataggcgga aacagatatt
     7381 ccatgatggc gaagaacgac gcgcgcagtt cctccggcac gatgtcgtgt tccttcaggt
     7441 agcgcaccga tcgatgtccg aactcctcga tgcccaggcg cgcgcggtga cggaacggtt
     7501 cggccgcgcc gagccggtgc agacggccga gataacggcc gagcagggcg cggtcctcgc
     7561 gcgagttcag ttctggcgcg cgccccggct gccacgggaa caccgcgaag cggaaaccgc
     7621 cgtggtaact gagcgtgttg ccgtcatgac gtctcaacgg cgcaacggcg gggatctcgt
     7681 gttcggccag ttgctgcgcg aaggtatgct cttccaggat ggcggcctcg tcccagcggc
     7741 cggggcggta aaacttggcc acgcacggcg gaccatcctc ggtgtccacg cgatagacgc
     7801 ggttttcgta gctgttgagc gcgagcaatc cgccggtgca gcgcacgccg tagagctcca
     7861 ccgcctcgag aatgatctcg ggtgtgagtg aatcgtaagg atgcggggct tcggcggtca
     7921 cggcggcacg ttataatagt tcgatatgga aaacacctct ctcaccagca acccactact
     7981 cgatcttgcg caaccgcccc gtttcggcgc cattcttccg gagcatgctg aaccggcgct
     8041 ggatcatgtt ctcgcggaaa atcgcgccgc cttggagcgc ctgctcgcgt cgggtggtcc
     8101 ttatacctgg gacaattttg cccagccgat cgaggacatg cgcgagcgcc tggcgcgcct
     8161 gtggtcgccg gtgtcgcacc tgcatgcggt catggacagc gaagcgctgc gcgccgccta
     8221 caatgccggc ctgcccaagc tcaccgccta tttcacggaa ctggcgcagg atgagcggct
     8281 ttacgccggt tataaatcca ttgccgcggg cccggaattc ccgaagctga cccaggcgca
     8341 aagaaagatc atcgagaata cgctgcggga ttttcgtctc gccggcgcgg agctgccgcc
     8401 gaaggaaaag gcgcgcctca aggcggtgca gcaggaactg gcggcgctcg ccagcaagtt
     8461 ctcggaaaac gtgctcgatg ccacccaggc atgggatctg cgcatcagcg acgaaaagga
     8521 tctggccggc ctgcccgagt cggcgcgcgc catggcgcga caggatgcac aggaaaaaaa
     8581 tctggagggc tggcatttta cgctcgaggg accgtcgtat atcgcgttca tgacctactc
     8641 ggatgatcgc gaacggcgcc ggcagatgta tgaggccttc gtcacgcgcg ccagcgatca
     8701 gggtccgacg gcgggcaaat gggacaacag cgagctgatc ctgaagctgt tacggttgcg
     8761 gcgcgaggcg gcgcggctgc tcggatttgg caatttcgcc gaatacgcac tgcaaacgcg
     8821 catggctaag aaggttccgg aggtaatgga ctttctgaac gatctggcgc gtcgcgcgaa
     8881 gcctgcggcg caaagagact tcgatgaact gaaacagttt gcccgcgagg cacacggcgt
     8941 cgaacgtctc gaagcctggg acatcgccta ctactccgag aagttgcagc aggcgaaata
     9001 ccgtatatcg caggaagacc tgcgtcccta ttttccggaa accaaagtgg tgcccggcat
     9061 gttcgaggtg gtcgagcggc tgtatggcct gaaagtcagc gcagtcaaag atgtcgaggt
     9121 gtggcatccg gacgtgcgct tttacgagat ccgcgatggt gcgggtgaag tgcgcgggcg
     9181 gttttatatg gacctgtacg cgcgcgccaa caagcgtggc ggcgcctgga tggacgaatg
     9241 catcaaccgc aagcgcacta cgggcggcgt gcaggtgccg gtggcgtatc tggtgtgcaa
     9301 cttcacgccg cccgtggccg gccggccggc gctgttcacg cacgatgagg tcatcaccct
     9361 gttccacgaa ttcggtcacg gcctgcacca catgttgacc aggatagatt acgtgggcgt
     9421 ggcgggcatc aatggcgtgg cttgggatgc ggtcgagctg ccgagccagt tcatggagaa
     9481 ctggtgctgg gaacgcgaag cactcaatct tatcgccagg catcacgaga gcggcgcaaa
     9541 aattccggac gagctttacg ccaaaatgat cgccgcgaag aacttccagt ccggcatgca
     9601 gttcgtgcgc cagttggagt tctcgctgtt cgacatgcgc ctgcacggcg cctacgaccc
     9661 ggatggtgcc gggactgtgc aacaggtcct ggacgaggtg cgcgcggagg tggcggtggt
     9721 gattccgccg gcatttaacc gcttccagaa cggcttttcg catatcttcg ccggtggtta
     9781 tgccgccggc tactatagtt acaagtgggc cgaggtgctg tcggcggatg ccttcagcaa
     9841 gttcgaggag aacggcgtgt tcgatcgtgc caccggactg cagtttttgc agaatatcct
     9901 cgaacagggt ggttcgcgcg aaccgatgga gctcttcatg aagttccggg ggcgcgcgcc
     9961 caagatcgat gcactgttgc ggcactccgg actggccgct taagggataa acacaatgac
    10021 aggaagtctg cgtgttatag cgattctggt gttgctggga atctgttccg ctgcccaggc
    10081 ggaaacggtt tacatcgccg aacgtgtccg catcgggttg cgcgccgaca tggatgaaac
    10141 cagtgcagtg gtgaaaacgg ttgagactgg cg
//
LOCUS       DBNGZE010000503         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Sulfuricaulis sp. isolate MAG05496
            SRR21617315_k127_1108489, whole genome shotgun sequence.
ACCESSION   DBNGZE010000503 DBNGZE010000000
VERSION     DBNGZE010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414904
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sulfuricaulis sp. (soil metagenome)
  ORGANISM  Sulfuricaulis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Acidiferrobacterales; Acidiferrobacteraceae; Sulfuricaulis.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,587
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,515
            CDSs (with protein)               :: 2,515
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 4 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 12 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Sulfuricaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1108489"
                     /isolate="MAG05496"
                     /isolation_source="soil"
                     /db_xref="taxon:2003553"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /gene="miaA"
                     /locus_tag="AC16FV_12925"
     CDS             complement(<1..146)
                     /gene="miaA"
                     /locus_tag="AC16FV_12925"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221110.1"
                     /note="IPP transferase; isopentenyltransferase; involved
                     in tRNA modification; in Escherichia coli this enzyme
                     catalyzes the addition of a delta2-isopentenyl group from
                     dimethylallyl diphosphate to the N6-nitrogen of adenosine
                     adjacent to the anticodon of tRNA species that read codons
                     starting with uracil; further tRNA modifications may
                     occur; mutations in miaA result in defects in translation
                     efficiency and fidelity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR2850988.1"
                     /translation="MSELPPAIFLMGPTAAGKTALALELHDMFPVEIISVDSSQVYRG
                     MDIGT"
     gene            complement(263..1762)
                     /gene="lysS"
                     /locus_tag="AC16FV_12930"
     CDS             complement(263..1762)
                     /gene="lysS"
                     /locus_tag="AC16FV_12930"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR2850989.1"
                     /translation="MSEEKQSLDENEQIAQRRSKLAELRQQGVPFPNDFRRNVIAGEL
                     HAEYGEKDPAEIDTRNVRVKVAGRMMTRRVMGKASFAHLQDMSGRIQIYVTRDAVGEP
                     AYEDFKKWDIGDIVGADGTLFKTKTGELSVKVDAVRLLAKALRPLPEKFHGLTDTETR
                     YRQRYLDLIMNEVARKTFRTRTAIIRYIREFLDGRGFLEVETPMMQVIPGGAAARPFV
                     THHHALDMGLYLRVAPELYLKRLIVGGFERVYEINRNFRNEGLSTRHNPEFTMLEFYQ
                     AYADYHDLMNLTEEMLRGLAREVLATETITYQGEQYDFSKRFQRMTLKQSILQSNSDI
                     KEQDLDSVDALRQIATRLGIQVKPNYGTGKLQLEIFEKTVEDKLKDPTFITAYPTEVS
                     PLARRNDTDPTITDRFEFFVGGREIANGFSELNDAEDQAERFRKQVAEKEAGDEEAMH
                     FDEDYIRALEHGMPPTAGEGIGIDRLVMLFTDSPSIRDVLLFPHMRPEK"
     gene            complement(1803..2916)
                     /gene="prfB"
                     /locus_tag="AC16FV_12935"
     CDS             complement(join(1803..2840,2842..2916))
                     /gene="prfB"
                     /locus_tag="AC16FV_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006084.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR2850990.1"
                     /translation="MEDLNPLFNKLRDLHDRSQVLRGYLDFDAKQHRLIEVQRELEQP
                     ELWNNPQRAQELGRERAKLESEVGTLTRLHTELTHSRELLELAREEGDAEVASSVAGD
                     LEKLEKELAGLEFRRMFSGEMDANNAFMDIQSGSGGTEAQDWANMLLRMYLMWGDRRG
                     FKTEILEISEGEVAGIKSATVKFTGPYAYGYLRTETGVHRLVRKSPFDSGNRRHTSFA
                     SVFAYPEADENIEVAINPADLRVDTYRASGAGGQHVNRTDSAVRITHIPTGIVVQCQS
                     DRSQHRNRASAMAMLKSRLFERELQARNKEKQKLEESKSDIEWGHQIRSYVLDQSRVK
                     DLRTGVESGNPEAVLNGDLDKFIDASLKSGLTETNT"
BASE COUNT          585 a          865 c          897 g          712 t
ORIGIN      
        1 gtgccgatgt ccatgccgcg atacacctgc gacgaatcca cgctgatgat ttccactgga
       61 aacatatcgt gcaattcgag cgcaagcgcg gtcttccccg ccgccgtcgg gcccatcaaa
      121 aaaatagcgg gagggagctc ggacatttag aaaaaatgga caggatttac aggattaaaa
      181 actggattaa caggatttaa aacataattt aaatatgcat taatcctgtc aatcctgaaa
      241 aatcctgtta atcctgtgaa atttattttt cagggcgcat gtgtgggaac aacagcacgt
      301 cgcggatcga aggcgaatcg gtaaacaaca tcaccaggcg gtcgatgccg ataccctcgc
      361 ccgccgttgg cggcatgccg tgttcgagcg cgcggatgta atcctcgtcg aagtgcatcg
      421 cttcctcgtc gccggcttcc ttctccgcca cctgtttgcg aaaacgctcg gcctggtcct
      481 cggcgtcgtt cagttcggaa aaaccgttgg cgatctcgcg tccgccgacg aaaaattcaa
      541 aacggtcggt gatcgtcggg tcggtgtcgt tgcgccgcgc cagcggcgag acttccgtgg
      601 gataggcggt gatgaaggtc ggatccttga gcttgtcctc caccgtcttc tcgaaaattt
      661 ccagttgcag cttgccggta ccatagtttg gcttcacctg aatccccagc cgcgtcgcga
      721 tctggcgcag ggcgtccacc gaatccaggt cctgttcctt gatatcggaa ttggattgca
      781 ggatggattg cttcagcgtc atgcgctgga agcgcttgct gaaatcatat tgctcgcctt
      841 ggtaagtaat ggtctcggtc gccagtactt cgcgcgccag gccgcgcaac atttcctcgg
      901 tcagattcat cagatcgtga tagtccgcat aggcctgata gaactcgagc atggtgaatt
      961 ccgggttgtg gcgcgtggac aggccctcgt tgcggaaatt gcggttgatt tcgtaaaccc
     1021 tctcgaagcc gccaacgatc aggcgcttga gatacagctc tggagccacg cgcaggtaca
     1081 gccccatgtc gagcgcgtga tgatgggtca cgaacggccg cgccgcggcg ccgcccggga
     1141 tcacctgcat catcggagtt tcgacctcga ggaagccgcg gccgtcgagg aactcgcgga
     1201 tatagcggat tatcgcggta cgcgtgcgga aggtcttgcg cgccacctcg ttcataatca
     1261 ggtcgagata gcgctgccgg taacgtgttt cagtatccgt caggccgtgg aatttctccg
     1321 gcagcggacg cagcgccttg gccagcagcc gcaccgcgtc caccttgacc gacagttcgc
     1381 cggttttggt cttgaacagc gtgccgtcgg cgccgacgat gtcgccgatg tcccatttct
     1441 tgaagtcctc gtaggcgggt tcgcctacgg cgtcgcgcgt gacgtaaatc tggatgcggc
     1501 cggacatgtc ctgcaggtgg gcaaagctgg ccttgcccat cacccggcgg gtcatcatgc
     1561 gtccggccac cttgacccgc acgttgcggg tgtcgatctc ggccgggtcc ttctcgccat
     1621 actcggcgtg caactcgcca gcgatgacgt tgcgccggaa atcgttgggg aatggaacgc
     1681 cctgctgacg cagctcggcg agcttggaac gccgctgggc gatctgctcg ttttcgtcca
     1741 ggctttgttt ttcctctgac atttatattg ttaattaaat atttaaactg attaagttga
     1801 tgttatgtat tagtttctgt aagcccgctt ttgaggctgg cgtcgataaa cttgtcgaga
     1861 tcgccgttca gcaccgcctc gggattgcca ctctccaccc cggtgcgcag atctttcacg
     1921 cgcgactggt cgagcacata cgagcgaatc tggtggcccc actcgatatc cgatttgctc
     1981 tcttccagct tctgcttttc cttgttgcgg gcctgcaatt cgcgctcgaa gagcctggac
     2041 ttcaacatcg ccatggccga tgcgcggttg cggtgctggg agcggtcaga ctggcattga
     2101 accacaatcc cggtcggaat gtgcgtgatg cgcaccgcag agtcggtgcg gttgacgtgc
     2161 tgcccgccgg cgccactggc gcggtaggtg tccacccgca gatcggccgg attgatggcc
     2221 acttcgatgt tttcgtcggc ctcgggatag gcaaaaaccg aggcaaacga ggtatggcgg
     2281 cggttgcccg aatcgaaggg cgacttgcgc accagacgat gcacgccggt ttcggtgcgc
     2341 aggtagccgt aggcgtatgg cccggtgaat ttgaccgtgg cgctcttgat gccggccacc
     2401 tcaccctcgg agatttccag gatttcggtc ttgaagccgc gccggtcgcc ccacatcagg
     2461 tacatgcgca gcagcatgtt ggcccaatcc tgggcctcgg tgccgccgga accggactgg
     2521 atatccatga acgcgttgtt ggcgtccatt tcgcccgaga acatgcgccg gaactcaagg
     2581 ccggcgagct ctttttccag tttctcgaga tcgccggcga cggaggaagc gacctcggcg
     2641 tcgccttcct cgcgcgccag ctccaacaat tcgcgtgagt gggtgagttc ggtatgtagc
     2701 cgcgtgagcg tgccgacctc ggactcgagt ttggcgcgtt cgcggccgag ttcctgcgcg
     2761 cgctgtgggt tgttccagag ttcgggttgt tcgagttcgc gctggacttc gatcaggcgg
     2821 tgctgcttgg cgtcgaagtc aaagataccc ccgtaagacc tgagaccggt cgtgcagatc
     2881 gcgcagcttg ttgaacaggg ggtttaggtc ttccattgga ttttcgtacc tgaataaacc
     2941 ttataacttt atttcttgaa atcagtctct tatagattct tgctaagata ctttttaaat
     3001 acaaaaatgt ccataaagct gtctcaaaaa cgtcgcgagc ggccgtcagc cgggtgagg
//
